LOCUS       DAETQL010000001        95533 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.001, whole genome shotgun sequence.
ACCESSION   DAETQL010000001 DAETQL010000000
VERSION     DAETQL010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.001"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(90..1340)
                     /gene="icd"
                     /locus_tag="K0W65_000001"
     CDS             complement(90..1340)
                     /gene="icd"
                     /locus_tag="K0W65_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBI3093448.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1512..2165
                     /gene="rluE"
                     /locus_tag="K0W65_000002"
     CDS             1512..2165
                     /gene="rluE"
                     /locus_tag="K0W65_000002"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBI3093449.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2175..2636
                     /gene="nudJ"
                     /locus_tag="K0W65_000003"
     CDS             2175..2636
                     /gene="nudJ"
                     /locus_tag="K0W65_000003"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBI3093450.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2690..3796
                     /gene="mnmA"
                     /locus_tag="K0W65_000004"
     CDS             2690..3796
                     /gene="mnmA"
                     /locus_tag="K0W65_000004"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBI3093451.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3832..4473
                     /gene="hflD"
                     /locus_tag="K0W65_000005"
     CDS             3832..4473
                     /gene="hflD"
                     /locus_tag="K0W65_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBI3093452.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4477..5847
                     /gene="purB"
                     /locus_tag="K0W65_000006"
     CDS             4477..5847
                     /gene="purB"
                     /locus_tag="K0W65_000006"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBI3093453.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6016..6687
                     /gene="phoP"
                     /locus_tag="K0W65_000007"
     CDS             6016..6687
                     /gene="phoP"
                     /locus_tag="K0W65_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBI3093454.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6687..8147
                     /gene="phoQ"
                     /locus_tag="K0W65_000008"
     CDS             6687..8147
                     /gene="phoQ"
                     /locus_tag="K0W65_000008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBI3093455.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8944..9225)
                     /locus_tag="K0W65_000009"
     CDS             complement(8944..9225)
                     /locus_tag="K0W65_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093456.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9239..10900)
                     /locus_tag="K0W65_000010"
     CDS             complement(9239..10900)
                     /locus_tag="K0W65_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBI3093457.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10884..11210)
                     /locus_tag="K0W65_000011"
     CDS             complement(10884..11210)
                     /locus_tag="K0W65_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBI3093458.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11266..11448)
                     /locus_tag="K0W65_000012"
     CDS             complement(11266..11448)
                     /locus_tag="K0W65_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093459.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11432..11872)
                     /locus_tag="K0W65_000013"
     CDS             complement(11432..11872)
                     /locus_tag="K0W65_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBI3093460.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11872..12168)
                     /locus_tag="K0W65_000014"
     CDS             complement(11872..12168)
                     /locus_tag="K0W65_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HBI3093461.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12165..12503)
                     /locus_tag="K0W65_000015"
     CDS             complement(12165..12503)
                     /locus_tag="K0W65_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HBI3093462.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12500..13711)
                     /locus_tag="K0W65_000016"
     CDS             complement(12500..13711)
                     /locus_tag="K0W65_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBI3093463.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13713..14285)
                     /locus_tag="K0W65_000017"
     CDS             complement(13713..14285)
                     /locus_tag="K0W65_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBI3093464.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14325..15482)
                     /locus_tag="K0W65_000018"
                     /pseudo
     CDS             complement(14325..15482)
                     /locus_tag="K0W65_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15783..16007)
                     /locus_tag="K0W65_000019"
     CDS             complement(15783..16007)
                     /locus_tag="K0W65_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093465.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16149..16310)
                     /locus_tag="K0W65_000020"
     CDS             complement(16149..16310)
                     /locus_tag="K0W65_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HBI3093466.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16320..16625)
                     /locus_tag="K0W65_000021"
     CDS             complement(16320..16625)
                     /locus_tag="K0W65_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093467.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16622..16900)
                     /locus_tag="K0W65_000022"
     CDS             complement(16622..16900)
                     /locus_tag="K0W65_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093468.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17189..18937)
                     /locus_tag="K0W65_000023"
     CDS             complement(17189..18937)
                     /locus_tag="K0W65_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBI3093469.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18934..19233)
                     /locus_tag="K0W65_000024"
     CDS             complement(18934..19233)
                     /locus_tag="K0W65_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBI3093470.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19239..19472)
                     /locus_tag="K0W65_000025"
     CDS             complement(19239..19472)
                     /locus_tag="K0W65_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093471.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19474..19694)
                     /locus_tag="K0W65_000026"
                     /pseudo
     CDS             complement(19474..19694)
                     /locus_tag="K0W65_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19687..19908)
                     /locus_tag="K0W65_000027"
     CDS             complement(19687..19908)
                     /locus_tag="K0W65_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093472.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19898..20098)
                     /locus_tag="K0W65_000028"
     CDS             complement(19898..20098)
                     /locus_tag="K0W65_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HBI3093473.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20228..20407
                     /locus_tag="K0W65_000029"
     CDS             20228..20407
                     /locus_tag="K0W65_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093474.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20532..20861
                     /locus_tag="K0W65_000030"
     CDS             20532..20861
                     /locus_tag="K0W65_000030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093475.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20911..21099)
                     /locus_tag="K0W65_000031"
     CDS             complement(20911..21099)
                     /locus_tag="K0W65_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBI3093476.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21474..22703)
                     /locus_tag="K0W65_000032"
     CDS             complement(21474..22703)
                     /locus_tag="K0W65_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBI3093477.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22952..24073
                     /gene="roxA"
                     /locus_tag="K0W65_000033"
                     /gene_synonym="ycfD"
     CDS             22952..24073
                     /gene="roxA"
                     /locus_tag="K0W65_000033"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HBI3093478.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24122..25348)
                     /gene="pepT"
                     /locus_tag="K0W65_000034"
     CDS             complement(24122..25348)
                     /gene="pepT"
                     /locus_tag="K0W65_000034"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBI3093479.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25598..26734
                     /gene="potA"
                     /locus_tag="K0W65_000035"
     CDS             25598..26734
                     /gene="potA"
                     /locus_tag="K0W65_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBI3093480.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26718..27575
                     /gene="potB"
                     /locus_tag="K0W65_000036"
     CDS             26718..27575
                     /gene="potB"
                     /locus_tag="K0W65_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBI3093481.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27572..28366
                     /gene="potC"
                     /locus_tag="K0W65_000037"
     CDS             27572..28366
                     /gene="potC"
                     /locus_tag="K0W65_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBI3093482.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28363..29409
                     /gene="potD"
                     /locus_tag="K0W65_000038"
     CDS             28363..29409
                     /gene="potD"
                     /locus_tag="K0W65_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBI3093483.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29565..30386)
                     /gene="cobB"
                     /locus_tag="K0W65_000039"
     CDS             complement(29565..30386)
                     /gene="cobB"
                     /locus_tag="K0W65_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBI3093484.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30402..31313)
                     /gene="nagK"
                     /locus_tag="K0W65_000040"
     CDS             complement(30402..31313)
                     /gene="nagK"
                     /locus_tag="K0W65_000040"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBI3093485.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31342..32586)
                     /gene="lolE"
                     /locus_tag="K0W65_000041"
     CDS             complement(31342..32586)
                     /gene="lolE"
                     /locus_tag="K0W65_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBI3093486.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32586..33287)
                     /gene="lolD"
                     /locus_tag="K0W65_000042"
     CDS             complement(32586..33287)
                     /gene="lolD"
                     /locus_tag="K0W65_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBI3093487.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33280..34479)
                     /gene="lolC"
                     /locus_tag="K0W65_000043"
     CDS             complement(33280..34479)
                     /gene="lolC"
                     /locus_tag="K0W65_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBI3093488.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34741..35814
                     /locus_tag="K0W65_000044"
     CDS             34741..35814
                     /locus_tag="K0W65_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBI3093489.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35942..39388
                     /gene="mfd"
                     /locus_tag="K0W65_000045"
     CDS             35942..39388
                     /gene="mfd"
                     /locus_tag="K0W65_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBI3093490.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39531..40493
                     /gene="ldtC"
                     /locus_tag="K0W65_000046"
     CDS             39531..40493
                     /gene="ldtC"
                     /locus_tag="K0W65_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HBI3093491.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40576..40788)
                     /locus_tag="K0W65_000047"
     CDS             complement(40576..40788)
                     /locus_tag="K0W65_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBI3093492.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            41073..41705
                     /gene="comR"
                     /locus_tag="K0W65_000048"
     CDS             41073..41705
                     /gene="comR"
                     /locus_tag="K0W65_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBI3093493.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41767..42306)
                     /locus_tag="K0W65_000049"
     CDS             complement(41767..42306)
                     /locus_tag="K0W65_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBI3093494.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42533..43837)
                     /gene="ndh"
                     /locus_tag="K0W65_000050"
     CDS             complement(42533..43837)
                     /gene="ndh"
                     /locus_tag="K0W65_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HBI3093495.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44245..44787)
                     /locus_tag="K0W65_000051"
     CDS             complement(44245..44787)
                     /locus_tag="K0W65_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI3093496.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44810..45835)
                     /gene="nagZ"
                     /locus_tag="K0W65_000052"
     CDS             complement(44810..45835)
                     /gene="nagZ"
                     /locus_tag="K0W65_000052"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBI3093497.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45846..46670)
                     /gene="thiK"
                     /locus_tag="K0W65_000053"
     CDS             complement(45846..46670)
                     /gene="thiK"
                     /locus_tag="K0W65_000053"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBI3093498.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46651..47292)
                     /gene="lpoB"
                     /locus_tag="K0W65_000054"
     CDS             complement(46651..47292)
                     /gene="lpoB"
                     /locus_tag="K0W65_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBI3093499.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47307..47684)
                     /locus_tag="K0W65_000055"
     CDS             complement(47307..47684)
                     /locus_tag="K0W65_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBI3093500.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47687..48046)
                     /gene="hinT"
                     /locus_tag="K0W65_000056"
     CDS             complement(47687..48046)
                     /gene="hinT"
                     /locus_tag="K0W65_000056"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBI3093501.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48385..50570
                     /gene="fhuE"
                     /locus_tag="K0W65_000057"
                     /pseudo
     CDS             48385..50570
                     /gene="fhuE"
                     /locus_tag="K0W65_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50630..52063)
                     /gene="ptsG"
                     /locus_tag="K0W65_000058"
     CDS             complement(50630..52063)
                     /gene="ptsG"
                     /locus_tag="K0W65_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBI3093502.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52358..53155)
                     /locus_tag="K0W65_000059"
     CDS             complement(52358..53155)
                     /locus_tag="K0W65_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBI3093503.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(53166..54170)
                     /gene="holB"
                     /locus_tag="K0W65_000060"
     CDS             complement(53166..54170)
                     /gene="holB"
                     /locus_tag="K0W65_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBI3093504.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(54167..54808)
                     /locus_tag="K0W65_000061"
     CDS             complement(54167..54808)
                     /locus_tag="K0W65_000061"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBI3093505.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54798..55820)
                     /gene="yceG"
                     /locus_tag="K0W65_000062"
     CDS             complement(54798..55820)
                     /gene="yceG"
                     /locus_tag="K0W65_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBI3093506.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55823..56632)
                     /gene="pabC"
                     /locus_tag="K0W65_000063"
     CDS             complement(55823..56632)
                     /gene="pabC"
                     /locus_tag="K0W65_000063"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBI3093507.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56752..57993)
                     /gene="fabF"
                     /locus_tag="K0W65_000064"
     CDS             complement(56752..57993)
                     /gene="fabF"
                     /locus_tag="K0W65_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBI3093508.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(58080..58316)
                     /gene="acpP"
                     /locus_tag="K0W65_000065"
     CDS             complement(58080..58316)
                     /gene="acpP"
                     /locus_tag="K0W65_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBI3093509.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58527..59261)
                     /gene="fabG"
                     /locus_tag="K0W65_000066"
     CDS             complement(58527..59261)
                     /gene="fabG"
                     /locus_tag="K0W65_000066"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBI3093510.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59274..60203)
                     /gene="fabD"
                     /locus_tag="K0W65_000067"
     CDS             complement(59274..60203)
                     /gene="fabD"
                     /locus_tag="K0W65_000067"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBI3093511.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60219..61172)
                     /gene="fabH"
                     /locus_tag="K0W65_000068"
     CDS             complement(60219..61172)
                     /gene="fabH"
                     /locus_tag="K0W65_000068"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HBI3093512.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61240..62310)
                     /gene="plsX"
                     /locus_tag="K0W65_000069"
     CDS             complement(61240..62310)
                     /gene="plsX"
                     /locus_tag="K0W65_000069"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBI3093513.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62391..62564)
                     /gene="rpmF"
                     /locus_tag="K0W65_000070"
     CDS             complement(62391..62564)
                     /gene="rpmF"
                     /locus_tag="K0W65_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBI3093514.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62616..63137)
                     /gene="yceD"
                     /locus_tag="K0W65_000071"
     CDS             complement(62616..63137)
                     /gene="yceD"
                     /locus_tag="K0W65_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBI3093515.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63336..63920
                     /gene="yceF"
                     /locus_tag="K0W65_000072"
     CDS             63336..63920
                     /gene="yceF"
                     /locus_tag="K0W65_000072"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBI3093516.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(64032..64991)
                     /gene="rluC"
                     /locus_tag="K0W65_000073"
     CDS             complement(64032..64991)
                     /gene="rluC"
                     /locus_tag="K0W65_000073"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBI3093517.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65564..68755
                     /gene="rne"
                     /locus_tag="K0W65_000074"
     CDS             65564..68755
                     /gene="rne"
                     /locus_tag="K0W65_000074"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBI3093518.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68952..69894)
                     /gene="flgL"
                     /locus_tag="K0W65_000075"
                     /pseudo
     CDS             complement(68952..69894)
                     /gene="flgL"
                     /locus_tag="K0W65_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69906..71548)
                     /gene="flgK"
                     /locus_tag="K0W65_000076"
                     /pseudo
     CDS             complement(69906..71548)
                     /gene="flgK"
                     /locus_tag="K0W65_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71614..72555)
                     /gene="flgJ"
                     /locus_tag="K0W65_000077"
     CDS             complement(71614..72555)
                     /gene="flgJ"
                     /locus_tag="K0W65_000077"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBI3093519.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72555..73652)
                     /gene="flgI"
                     /locus_tag="K0W65_000078"
     CDS             complement(72555..73652)
                     /gene="flgI"
                     /locus_tag="K0W65_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBI3093520.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTVSLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73664..74362)
                     /gene="flgH"
                     /locus_tag="K0W65_000079"
     CDS             complement(73664..74362)
                     /gene="flgH"
                     /locus_tag="K0W65_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBI3093521.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74415..75197)
                     /gene="flgG"
                     /locus_tag="K0W65_000080"
     CDS             complement(74415..75197)
                     /gene="flgG"
                     /locus_tag="K0W65_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HBI3093522.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75335..76086)
                     /gene="flgF"
                     /locus_tag="K0W65_000081"
                     /pseudo
     CDS             complement(75335..76086)
                     /gene="flgF"
                     /locus_tag="K0W65_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(76106..77314)
                     /gene="flgE"
                     /locus_tag="K0W65_000082"
     CDS             complement(76106..77314)
                     /gene="flgE"
                     /locus_tag="K0W65_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HBI3093523.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(77339..78034)
                     /gene="flgD"
                     /locus_tag="K0W65_000083"
     CDS             complement(77339..78034)
                     /gene="flgD"
                     /locus_tag="K0W65_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HBI3093524.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(78046..78449)
                     /gene="flgC"
                     /locus_tag="K0W65_000084"
                     /pseudo
     CDS             complement(78046..78449)
                     /gene="flgC"
                     /locus_tag="K0W65_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78453..78869)
                     /gene="flgB"
                     /locus_tag="K0W65_000085"
     CDS             complement(78453..78869)
                     /gene="flgB"
                     /locus_tag="K0W65_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBI3093525.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            79024..79683
                     /gene="flgA"
                     /locus_tag="K0W65_000086"
     CDS             79024..79683
                     /gene="flgA"
                     /locus_tag="K0W65_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBI3093526.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79759..80052
                     /gene="flgM"
                     /locus_tag="K0W65_000087"
     CDS             79759..80052
                     /gene="flgM"
                     /locus_tag="K0W65_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBI3093527.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            80057..80473
                     /gene="flgN"
                     /locus_tag="K0W65_000088"
     CDS             80057..80473
                     /gene="flgN"
                     /locus_tag="K0W65_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBI3093528.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80513..82048)
                     /gene="murJ"
                     /locus_tag="K0W65_000089"
     CDS             complement(80513..82048)
                     /gene="murJ"
                     /locus_tag="K0W65_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBI3093529.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82248..83081)
                     /locus_tag="K0W65_000090"
     CDS             complement(82248..83081)
                     /locus_tag="K0W65_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBI3093530.1"
                     /translation="MKKLRIGVVGLGGIVQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(83083..83730)
                     /locus_tag="K0W65_000091"
     CDS             complement(83083..83730)
                     /locus_tag="K0W65_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HBI3093531.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83741..84325)
                     /gene="rimJ"
                     /locus_tag="K0W65_000092"
     CDS             complement(83741..84325)
                     /gene="rimJ"
                     /locus_tag="K0W65_000092"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBI3093532.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84561..85769
                     /gene="mdtH"
                     /locus_tag="K0W65_000093"
     CDS             84561..85769
                     /gene="mdtH"
                     /locus_tag="K0W65_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBI3093533.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85833..86480
                     /gene="grxB"
                     /locus_tag="K0W65_000094"
     CDS             85833..86480
                     /gene="grxB"
                     /locus_tag="K0W65_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBI3093534.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86614..87174
                     /locus_tag="K0W65_000095"
     CDS             86614..87174
                     /locus_tag="K0W65_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI3093535.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87280..88326
                     /gene="pyrC"
                     /locus_tag="K0W65_000096"
     CDS             87280..88326
                     /gene="pyrC"
                     /locus_tag="K0W65_000096"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBI3093536.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88400..88645
                     /gene="dinI"
                     /locus_tag="K0W65_000097"
     CDS             88400..88645
                     /gene="dinI"
                     /locus_tag="K0W65_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBI3093537.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88935..89189
                     /gene="bssS"
                     /locus_tag="K0W65_000098"
     CDS             88935..89189
                     /gene="bssS"
                     /locus_tag="K0W65_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBI3093538.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            89304..90422
                     /gene="solA"
                     /locus_tag="K0W65_000099"
     CDS             89304..90422
                     /gene="solA"
                     /locus_tag="K0W65_000099"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBI3093539.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90470..90583
                     /locus_tag="K0W65_000100"
     CDS             90470..90583
                     /locus_tag="K0W65_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HBI3093540.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90844..91410
                     /locus_tag="K0W65_000101"
     CDS             90844..91410
                     /locus_tag="K0W65_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBI3093541.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            91414..91989
                     /locus_tag="K0W65_000102"
     CDS             91414..91989
                     /locus_tag="K0W65_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBI3093542.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(92031..93083)
                     /locus_tag="K0W65_000103"
     CDS             complement(92031..93083)
                     /locus_tag="K0W65_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBI3093543.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            93308..94228
                     /gene="lpxL"
                     /locus_tag="K0W65_000104"
     CDS             93308..94228
                     /gene="lpxL"
                     /locus_tag="K0W65_000104"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HBI3093544.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            94400..94597
                     /locus_tag="K0W65_000105"
     CDS             94400..94597
                     /locus_tag="K0W65_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBI3093545.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94621..>95533
                     /locus_tag="K0W65_000106"
     CDS             94621..>95533
                     /locus_tag="K0W65_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI3093546.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIK"
BASE COUNT        22991 a        25314 c        23569 g        23659 t
ORIGIN      
        1 aatatgctcg atatgctatt aataatatcc acataaataa aacaacgggc gtgttatacg
       61 cccgtttaaa tatttaacac atgtagtgat tacatattct tgatgatcgc atcaccaaac
      121 tctgaacatt tcagcagttt agcgccttcc atcagacgct cgaagtcata ggttacggtc
      181 ttggcgttga tcgcgccttc cataccttta acaatcaggt ctgcggcttc agtccagccc
      241 atatggcgta acatcatctc agcggagaga ataatagagc cagggtttac tttgtcctga
      301 ccggcgtatt tcggcgcagt accgtgggtg gcttcaaaca gggcgcattc gtcaccgatg
      361 tttgctccag gggcgatacc gataccgcca acctgcgctg ccagagcgtc agaaatgtag
      421 tcaccgttca ggttcataca ggcgataaca tcatattcag ccggacgcag caggatctgt
      481 tgcaggaatg catcagcaat cacgtcttta atgacgatct ctttgccggt attcgggttt
      541 ttaactttca gccacgggcc gccgtcgatc agttcaccgc caaattcttc acgcgccagc
      601 tggtagcccc agtctttaaa cgcgccttcg gtgaacttca tgatgttgcc tttgtgcacc
      661 agggtcacag agtcacgatc gttagcaatt gcgtattcga tcgctgcacg aaccagacgt
      721 ttggtgcctt cttcagaaca cggcttaata ccgataccgc aatgttccgg gaagcgaatt
      781 ttcttcacgc ccatctcttc acgcaggaat ttaatcactt tctcggcgtc ggcagagtca
      841 gctttccatt cgatacccgc ataaatgtct tccgagtttt cacggaagat aaccatatcg
      901 gtcagttcag ggtgtttaac cgggcttgga gtgccctgat agtaacgtac cggacgcagg
      961 cagatgtaga gatccagttc ctgacgcagg gcaacgttga gagaacgaat accgccacca
     1021 accggagtgg tcagcgggcc tttaatggca acgcgatatt cacgaatcag atcaagggtt
     1081 tcagcaggca gccagacatc ctgaccataa acctgtgtgg atttttcacc ggtgtaaatt
     1141 tccatccagg agattttacg ctcgccttta taggctttct cgactgcagc gtcgaccact
     1201 ttcagcatgg ctggggttac atctacaccg attccatcac cttcaatgta agggataatc
     1261 ggattttcag gaacgttgag tttgccgttt tgcagggtga tcttcttgcc ttgtgccgga
     1321 acaactactt tactttccat tcacctctcc ttcgagcgct actggtttgc tcgtctgcca
     1381 ccacgttgca tatgcgtttg cgtcctgcga tacggatgct ttagagcaat tttttgttaa
     1441 tgatttgtaa ttgacttgtc aatactaccc tattgtttgc cgtcatgaaa gatgcgcgtt
     1501 attaggctat aatgcggcaa ttcataatct ctgaaaatac catgcaaaaa acttctttta
     1561 gaaatcacca ggttaagcga ttcagctcgc aacgttctac caggcgtaaa catgaaaacc
     1621 agcccacgcg tgtgatcctc ttcaataaac cctacgatgt tcttccgcag ttcaccgatg
     1681 aaaccggacg caaaacatta aaagaattca tcccggttca gggcgtttat gcagcaggtc
     1741 gccttgaccg cgatagcgaa gggttgctgg tgctgaccaa taacggcgcg ttgcaggcgc
     1801 gtttaaccca gccgggtaaa cgcaccggaa aaatctatta tgtgcaggtg gaaggtattc
     1861 ccacacaaga cgcacttgaa gccttgcgca atggcgtaac cttaaatgat ggccctaccc
     1921 tgcccgcagg cgcggagctg gttgacgaac ccgcgtggtt atggccgcgg aatccaccaa
     1981 ttcgtgaacg caaaagtatt cccaccagct ggctgaagat caccttatat gaaggacgta
     2041 atcgccaggt gcgccgcatg accgcccatg ttggtttccc cacgctgcga ctgattcgct
     2101 atgcgatggg agattactct ttggataatc ttgccaatgg cgaatggcga gaagtgacag
     2161 attaaggatg caaaatgttt aaaccgcacg ttaccgttgc ttgcgtggtg cacgcagaag
     2221 gcaaattttt agtcgttgaa gagacgatta atggtaaagc gttatggaac caacctgccg
     2281 ggcatctgga agccgatgaa accttagtgg aagccgccgc ccgtgaactg tgggaagaaa
     2341 ccggcatcag cgcgcagccg caacacttta ttcgtatgca tcagtggatt gcgccagata
     2401 aaacgccgtt tttgcgtttc ctctttgcca ttgagcttga gcaaatatgc ccgacccagc
     2461 ctcatgacag cgatatcgac tgctgccgtt gggtcagcgc cgaagaaatt ttacaggcgt
     2521 caaatcttcg ttcgccgctg gtggcggaaa gtattcgttg ttatcaaagc gggcaacgtt
     2581 atccgctgga gatgattggc gattttaact ggccttttac aaagggtgtc atctaaaagg
     2641 ctgcgtgata gaatacgccg ccttgaagtt caatgtcgtg agtgatccaa tgtctgaaac
     2701 cgcaaaaaaa gtaatcgtcg gcatgtccgg cggtgtcgat tcctccgttt ctgcctggct
     2761 gttgcaacaa cagggatatc aggtcgaagg cctgtttatg aagaactggg aagaagacga
     2821 cggtgaggaa tattgcacag cagcagcgga tctggctgat gcccaggcgg tctgcgacaa
     2881 gctcggcatt gaactgcaca ccgttaactt tgctgccgag tactgggaca acgtcttcga
     2941 actgttcctt gccgaatata aagccggtcg cacgccgaat ccggatattc tgtgcaacaa
     3001 ggagatcaaa tttaaagcct tcctcgaatt tgccgccgaa gatttaggtg ccgattatat
     3061 cgctaccggt cattacgtac gtcgggccga tgtcgatggc aagagccgcc tgctgcgtgg
     3121 tctggacagc aataaagacc agagctactt cctttatacg ctcagccatg agcagattgc
     3181 gcaaagcctg ttcccggtag gcgaactgga aaaaccgcag gtgcgtaaga ttgctgaaga
     3241 tcttggtctg gtcaccgcga agaaaaaaga ctctaccggc atttgcttca ttggcgaacg
     3301 taaattccgc gagttcctgg gccgttatct cccggcgcaa ccgggcaaaa tcattaccgt
     3361 cgatggcgat gaaattggtg agcaccaggg gctgatgtat cacactctcg gtcagcgtaa
     3421 aggtctgggt atcggtggca ccaaagaagg taccgaagaa ccgtggtatg tggtggacaa
     3481 agacgtcgaa aacaacattc tggttgtcgc tcagggccat gaacacccgc ggctgatgtc
     3541 tgtcgggttg attgcccagc agctgcactg ggtcgatcgc gaaccattca ccggcactat
     3601 gcgttgcacg gtaaaaaccc gctatcgcca gaccgacatt ccgtgcaccg tcaaggcgct
     3661 ggacgacgat cgcattaaag tgattttcga tgagccggtt gccgccgtga cgccgggcca
     3721 gtctgccgtc ttctataacg gtgaagtgtg cctcggtggc ggtattattg agcagcgtct
     3781 gccgctgccg gtctgattat tatctttact taaaacaagg aagcagtgaa cgtggcaaag
     3841 aattactatg acatcaccct cgccctggcc ggtatttgtc agtcggcacg cctggtgcaa
     3901 caactcgctc accaggggca ttgtgatgcc gatgcgctac acgtctcact caacagtatt
     3961 attgatatga accccagctc gacgctggcg gtttttggcg gtagcgaagc caacctgcgc
     4021 gtcgggctgg aaaccctgct cggcgtgctc aatgccagca gttgccaggg cttaaacgcc
     4081 gaattaaccc gctacacact cagcttgatg gtgcttgagc gcaaactctc ctcagcgaaa
     4141 ggcgcgctcg acactctggg caaccggatc aacggcctgc aacgccagct cgaacacttc
     4201 gatttacagt ccgaaacgct gatgagcgcg atggctgcta tctatgttga tgtgatcagc
     4261 ccgcttggcc cgcgcattca ggtcaccggt tcccctgctg tactgcaaag cccacaagtg
     4321 caggcgaaag ttcgcgcaac cctgctggca ggcattcgcg ccgccgtgct ctggcaccag
     4381 gtcggcggcg gacgtctaca actgatgttt tctcgtaatc gcctgaccac tcaggcaaaa
     4441 caaattcttg ctcatttaac cccggagttg tgatctatgg aattatcctc actgaccgcc
     4501 gtttcccctg tcgatggacg ctacggcgat aaagtcagcg cgctgcgcgg gattttcagc
     4561 gaatatggtt tgctgaaatt ccgtgtacaa gttgaagtac gttggctgca aaaactggcc
     4621 gcgcacgcag cgatcaagga agttcctgct tttgctgccg acgcaatcgg ttaccttgat
     4681 gcaattgtcg ccaatttcag cgaagaagat gccgcacgca tcaaaaccat cgagcgtacc
     4741 actaaccacg acgttaaagc ggttgagtat ttcctgaaag aaaaagtggc ggagatcccg
     4801 gaactgcacg cggtttctga attcatccac tttgcctgta cttcggaaga tatcaataac
     4861 ctctcccacg cattaatgct gaaaaccgcg cgtgatgaag tgatcctgcc gtactggcgt
     4921 caactgattg atggcattaa agatctcgcc gttcagtacc gcgatatccc gctgctgtct
     4981 cgtacccacg gtcagccagc cacgccgtca accatcggta aagagatggc aaacgtcgcc
     5041 taccgtatgg agcgccagca ccgccagctt aaccaggtgg agatcctcgg caaaatcaac
     5101 ggcgcggtcg gtaactataa cgcccacatc gccgcttacc cggaagttga ctggcatcag
     5161 ttcagcgaag agttcgtcac ctcgctgggt attcaatgga acccgtacac tacccagatt
     5221 gaaccgcacg actacattgc cgaactgttt gattgcgttg cgcgcttcaa caccattctg
     5281 atcgactttg accgtgacgt ctggggttat atcgccctta accacttcaa acagaaaacc
     5341 attgctggtg agattggttc ttccaccatg ccgcataaag ttaacccgat cgacttcgaa
     5401 aactccgaag ggaatctggg cctttccaac gcggtattgc agcatctggc aagcaaactg
     5461 ccggtttccc gctggcagcg tgacctgacc gactccaccg tgctgcgtaa cctcggcgtg
     5521 ggtatcggtt atgcgctgat tgcgtatcaa tccaccctga aaggcgtgag caaactggaa
     5581 gtgaaccgtg accatctgct ggatgagctg gatcacaact gggaagtgct ggcagaacca
     5641 atccagacag ttatgcgtcg ctatggcatc gaaaaaccgt acgagaagct gaaagagctt
     5701 actcgcggta agcgcgttga cgccgaaggc atgaagcagt ttatcgatgg tctggcgttg
     5761 ccagaagaag agaaagcccg cctgaaagcg atgacgccgg ctaactatat tggtcgagct
     5821 atcacgatgg ttgatgagct gaaataagcc tcaaatcagt gccggatggc gatgctgtcc
     5881 ggcctgctta ttaagattat ccactttttc ttttttcact ttacctcccc tccccgctgg
     5941 tttatttaat gtttaccccc ataaacacat aatcgcgtta cactatttta ataattaaga
     6001 cagggagaaa taaaaatgcg cgtactggtt gttgaagaca atgcgttgtt acgtcaccac
     6061 cttaaagttc agattcagga tgctggtcat caggtcgatg acgcagaaga tgccaaagaa
     6121 gccgattatt atctcaatga acatttaccg gatattgcga ttgtcgatct cggattgcct
     6181 gacgaggacg gtctgtcact gattcgccgc tggcgtagca acgatgtttc actgccgatt
     6241 ctggtattaa ccgcccgtga aagctggcag gacaaagtcg aagtattaag tgccggtgct
     6301 gatgattacg tgactaaacc gtttcatatt gaagaggtga tggcgcgaat gcaggcatta
     6361 atgcggcgta atagcggtct ggcttcacag gtcatttcgc tccccccgtt tcaggttgat
     6421 ctctctcgcc gtgaattatc tattaatgac gaagtgatca aactgaccgc gttcgaatac
     6481 accattatgg aaacgttgat acgcaataat ggcaaagtgg tcagcaaaga ttcgttaatg
     6541 ctacaactct atccggatgc ggagctgcgg gaaagccata ccattgatgt actgatggga
     6601 cgtctgcgca aaaaaattca ggcacaatat ccgcaagaag tcattaccac cgttcgcggc
     6661 cagggctacc tgttcgaatt gcgctgatga aaaaattact gcgtcttttt ttcccgctct
     6721 cgctgcgggt acgttttctg ttggcaacgg cagcggtagt actggtgctt tcgcttgcct
     6781 acggaatggt cgcgctgatc ggttatagcg tcagtttcga taaaactacg tttcggctgt
     6841 tacgtggcga gagcaatctg ttctataccc ttgcgcagtg ggaaaacaat aagttgcatg
     6901 tcgagttacc cgaaaatatc gacaagcaaa gccccaccat gacgctaatt tatgatgaga
     6961 acgggcagct tttatgggcg caacgtgacg tgccctggct gatgaaaatg atccagcctg
     7021 actggctgaa atcgaatggt tttcatgaaa ttgaagcgga tgttaacgat accagcctct
     7081 tgctgagtgg agatcattcg atacagcaac agttgcagga agtgcgggaa gatgatgacg
     7141 acgcggagat gacccactcg gtggcggtaa acgtctaccc ggcaacatcg cggatgccaa
     7201 aattaaccat tgtggtggtg gataccattc cggtggagct aaaaagttcc tatatggtct
     7261 ggagctggtt tatctatgtg ctctcagcca atctgctgtt agtgatcccg ctgctgtggg
     7321 tcgccgcctg gtggagttta cgccccatcg aagccctggc aaaagaagtc cgcgaactgg
     7381 aagaacataa ccgcgaattg ctcaatccag ccacaacgcg agaactgacc agtctggtac
     7441 gaaacctgaa ccgattgtta aaaagtgaac gcgaacgtta cgacaaatac cgtacgacgc
     7501 tcaccgacct gacccatagt ctgaaaacgc cactggcggt gctgcaaagt acgctgcgtt
     7561 ctctgcgtag tgaaaagatg agcgtcagtg atgctgagcc ggtaatgctg gagcaaatca
     7621 gccgcatttc acagcaaatt ggctactacc tgcatcgtgc cagtatgcgc ggcgggacat
     7681 tgctcagccg cgagctgcat ccggtcgccc cactgctgga caatctcacc tcagcgctga
     7741 acaaagtgta tcaacgcaaa ggggtcaata tctctctcga tatttcgcca gagatcagct
     7801 ttgtcggtga gcagaacgat tttgtcgagg tgatgggcaa cgtgctggat aatgcctgta
     7861 aatattgcct cgagtttgtc gaaatttctg caaggcaaac cgacgagcat ctctatattg
     7921 tggtcgagga tgatggcccc ggtattccat taagcaagcg agaggtcatt ttcgaccgtg
     7981 gtcaacgggt tgatacttta cgccctgggc aaggtgtagg gctggcggta gcccgcgaaa
     8041 tcaccgagca atatgagggt aaaatcgtcg ccggagagag catgctgggc ggtgcgcgga
     8101 tggaggtgat ttttggtcgc cagcattctg cgccgaaaga tgaataaata tgtcctttta
     8161 ccactacatc aagtcattga ttcgatcaaa caattttcat tgaaaaagtg ccaaaaatcg
     8221 caaattgact acaccattaa ctacaccgtt cggtgcactg tatgaaacaa cgtggaacaa
     8281 atagacacaa gaaatataca ggcgggtaat ctttccaggg ggaagcgcac caattcatga
     8341 ggggcgttaa tgtcgatatg gggatcccca taacggggct accggatttt ttttccggtt
     8401 aacctttaat caggctggtg ggttttacat acccttgatc accgtaatga tgatccagat
     8461 gtgcaccttt tccaccaatg gcggaaaagc tccaaccgta acgatgatcg ctatggttgc
     8521 ctcgatggtc tgcgtggtca ttatgaccgt tcagccagtg accttaatca gaatttttcg
     8581 ctaaaaaatg acgttggcca cgtcatccgg aaagcacaaa atcacgtcga tttttaatga
     8641 acggttaaac cgtctttttg tcacggtgtg tcactggttg tcactctccg tcacgattga
     8701 ggtggtggtc attctgcccg accgcgcatc tttgcgctat cgggaatatc aaacagttac
     8761 ctcgccgact tcctcaaatt gaggtttccg aaaatatcag agcgggttag cgattaagct
     8821 gacgagtaat taaactgcga aaatttcgta gtttctgaat atattccggc ctgctggtta
     8881 acagtcctca gatgtgaggg atgtagccag cgcaattttg cgctttgctt taatctcagt
     8941 gagttaccgc cgtaaccgct ccggcttctt ccagtggtac gtgattttct ccttctcccg
     9001 atacagttca acgcggcgat tgtaggccag catttccaga acgcggatcc gtatgtcgcg
     9061 catatccgca tcattaagct ggataccatc acggcgcatc acctcagcaa caacacgcgc
     9121 ataattttcg gctgtcacgc tgtccggctg cgtggtctgt tcgtcagcct gctggctgat
     9181 tccagagacg cggcggatta atcgcagtat ttcggcttct gtcatgctgc tgacctcatt
     9241 acaccccgct aaattcttcc agtttctggc ggtggctgtc gcttatatca aaagcaaaat
     9301 cctcatgctc tgcctggaat gtaccaaacg ccatcagcgc cgctacgctc gggtctatct
     9361 tgttcggtga ttttttcttg ttcggcttga tattggcgtt cgcgtcactc tgcatcacga
     9421 cgttactcat cgaccaggac aacaccggat cgccacgatg cacaatcacc ctgcggttaa
     9481 caaaaacttc gaacgatttc gccgccggac tgaatctgag gtaggtttgc gggaacggct
     9541 ccacctcaaa accagccccc tgtaattgcg tcctgagatg cgtggcgttc cacgtatcaa
     9601 agcccaccag cctgatatta aatttctccg catcctgcat gatgtcatct ctgatccggt
     9661 cgtaatcaat gcagtcacct ggcgttgtgc gtatccagcc cgctttagcc cactggcgat
     9721 agacagcgcg gtttttattg gcggggttct gtagctggaa ctccggcaga taatgacggg
     9781 aaatcagcat aatggtttta ccgaccggaa aggcatagca tacgctggaa atatcgctgg
     9841 ttgatgataa gtccagcccc gcgtaacact cctgaccgtg taaatcttcc tccgtgaacg
     9901 ttccggcaca ctcagcccat gcaccattac ccatccacgg ggtagtcccc tgacaccaga
     9961 tattaaatcg ctttgtcatc atctccaccc actgcgacgg aataccccgc gctttctgga
    10021 tggttgaggc cagtttttca cgatccacgg aaacatcaat attagggttc gcctttatcc
    10081 acatcgccgg atcatcaacc tcgttttcgt cgtccagttc gtaaatcagt acaaaaattg
    10141 aatcgttaac ctcttcgccg tccaggatct ggcagcaata atcatagtgc tgtttacagg
    10201 ctgaaacgac gttactgccc gatgtggtaa tggcaaataa cagcccttcg ggacgtgcgc
    10261 ccatccccag ctcaagcgcg gaataaacgc cgttatcggg gtgtaggtgg tattcgtcca
    10321 cgatggaaag actggggttt gtcccctcga tggttgccgc ttttgctgcc agcggcttta
    10381 acaggctgtt gcttttcggg tgtatcacct tatgcgcctg aatattcacc cgcctgcgta
    10441 acggtcggga taaaaggcac atctgacgcg cattatcaaa cacgatccgc gcctggtcac
    10501 ggctcaccgc tgcggtgtag atatcctgct gaccgttctc cataatcaga aaccagttag
    10561 ccagaatcgc ggcggtcgtg gatttcgcat ttttgcgcgg cacttcgata aaggcgctcg
    10621 tgtatttgcg ccgtccggtg gccttaacct taaagccgag gatgcacgca aaggcgaact
    10681 gctgccacgg ctccagctca atgggtctgc cacgcatcgg tccttttacg tgcgggcaca
    10741 ccctggaaaa ggcaataaac cgctccacaa cctcacgatc gaacgtgtaa agggggcttt
    10801 taaggtccga aaagtaccgt ttaacagcct gttttaaccg tttacaggcc ggaattttgc
    10861 ccgtttttac gtcttctgcg tacttattcc aggcggtcaa gctcgtcctc ttcttctgtt
    10921 tccggtggat ttttacggcg gcttatcggg tcaaaaccga gcaaggaggc catttttatc
    10981 atcactcttt cagcgtcgga ttttgcgctt aatgcggggt ttctgctctc gccgccctga
    11041 ctgttaacaa tgctgaaccc gcgcgccgca aggtctgcaa cggctttccg gtaaatggag
    11101 tagttgacgc aatacagttc cagattgctc cagtcggcgg gggtcaggtc tccccgttcc
    11161 gcaagctgcc gcgatttttc ccgccactgc ttcacggcga tatcatccag gtaggcgggg
    11221 tcgggtttgt cctgaaaacg ccccccaccc cgtcatacag cctcatcagc gattgcggaa
    11281 acattccata acctcgcggt cacggtcggt taatcgcttc gctgtgatgc gttctgcgcg
    11341 tcctgacgct ttatctttat gccctgtttc ctgtgtcttc catgcgtcac gctgccttat
    11401 aagtccacgg ataaggcggt tttgttcccg ctcattcatc agcgtcatac atccagttat
    11461 tgcggttagc agcccgttct tcctcttcac gaaatccacc tgcggcacgc ttgctttttg
    11521 ttgccggatc aagccatttc gttttctggt tatgacacgc ctggcacaat ggttgatggt
    11581 tccattcggg ccagaagaga acatcatcac cgccattaat cgggataatg tgatccacca
    11641 ccacggcggg cgtatatatc cccttctcaa ggcatcgcac gcataacggg tttttactca
    11701 gatacatggc gcggtatttg tcccactgtc tggaatatcc acgcgcgcgg cggtgtcctc
    11761 gtctggcatc ctctgcacgc catgccgccc gcctgtgctc ctcacacttg ccggatttaa
    11821 cgcgcctgtt acagcctggt tctgtgcatc gtcttaatgg ttgccacggc atcagtaccc
    11881 ccccacatca cggtaagccg tccagagtgc gcctatcgtc atgggtacgc gtgtttttgc
    11941 gttatccgcg acaatctggc gattttcata caggtgagcg ataaacatca tgcagccaat
    12001 ctttatggct ggcgtgaacg ccagcccgtc atcaaagcgc ctgcctatgt gtatctggca
    12061 cgcctcaagc gatgcggcaa tgtatccgct gattaactgg tcttcatcgt cgccatcgat
    12121 gcggcaatgg agtttcactt cttccagggt gataagttct tctgtcattt ttccgcgccc
    12181 tcacgacaaa gaatttcaag gcgtgtcctg gcggcatccg gcagcggctg cccgatgata
    12241 ttcagtacac gccccgccag cggccccgta ctgaccttta tccggctggt ggcgttgatg
    12301 tccttccggt accgtatcca gatccttacg gtaccaatcg ccagctctgc gcccgatgaa
    12361 atggcctcct tgctgctgac catgttcacg cttgcccaga gtgtgtgacc gtcctcccac
    12421 gtttcgagta tttcgccggt tatatccctg gtctgtttca gggtctgaat cgttatcctg
    12481 tcacgcagtc gccctatgtt cattccggtt tttctccctc gctgattttt acttcctgtt
    12541 tccatgcctg gctgaactca tcaccaccat cacgcggtga cagcccttca cgttcgcggg
    12601 cttcgttcgg acacataacg tcggatttta tgccgcgctc ataggtggca aagcgttcgc
    12661 caggtgtggc ccgtaacagg tccgcgctgt caaactccac ctgataccgg ataccaggta
    12721 caggcgaggc caccagcaag gcgtttttta tctgctgctc aaagttcgcc agccacgggc
    12781 gcatcgtcat ggtgagaaat gcgcggcttg cctcgctgaa gttgctgtag gtgctgttgc
    12841 tgtattcctg caaaaaaatc ggcgagacgt tgaacattcg ggcgatgtct tcaatggtga
    12901 agcgacggga ggccagccat tcggcatcct gattactcat gcccagctgc tgatagctca
    12961 tccccccttc aaggatgggc gtttttccgg cgtttctggc ccctttgtag cgttccagtg
    13021 cggctaatgc ctgtttgccc ttcacgccgt ccagccattc gcctgacgtg ataacccctg
    13081 ccgccatcat gccatcacgc atcacgctcg cgccgtggcg ttgttgggcc agcccaagcc
    13141 ccagcgattc gcggcagatg gttacaggtg agcgccccat aaaaccgtca tccgtggagt
    13201 aacgaaggtg gagaacctcc cacggtaaat agttgcggat gtttccggtg taggcgtcag
    13261 tgatgcagta gcgccagtta tgatcacctg tctgctccac gttcaccgac tgcggcgggt
    13321 aaggatgtaa agccgccgga aaaccatcac gcccccactg aataaccgca taagcattac
    13381 cacttaacag gcagtagcgg atcatcattc gcttaaactg gtagggtgtc tgccacgcgt
    13441 tcgggcgctc gttgaggatg tgatcgaccg gatgagaatc aagccattcg cgggcctcct
    13501 tccccttctc attgcgtacc aggtacaggt aacacggcat ggtagccacc gcgtcagaga
    13561 tgaccgtgac ggcgttcatg acggcgggga gtgattccgc tgtcccagat gatacgtact
    13621 cacccgcccc cgtgttcgat gtgccggcca gcgccataaa ttcatcaagc gtcatgctgc
    13681 gctgctcttt ttttcgtctg aaaggccaca tatcacaccc ccgctaaatc cgcccaccag
    13741 cggcgattat ccgcacgcgg cattttttcg gggtgctgct catacaggga acggcgggcc
    13801 agctccacgc cggaatcggg gtaagccggt accgatgtaa cggtaatttc gtacagttcc
    13861 gccaccagca cggtgcgcac gcatggatct gttgtggtat cccatacatc cttacgggag
    13921 cgaaagccaa agctcatccc cgagatgtcc ccacgcttaa ccagttcgat aacgtcgcgc
    13981 cctgtactgg tatccggtgg ggtcagttca aagcgtaacc ctgtttcgtc ctcttccagt
    14041 ttcagcgtgc cggaacgggt gcgccccagt aacatgctgt ggtcatgctc atacaggccg
    14101 cgaacgtcat tacccgccgc aagccattca gtaaacgccc cccgctggaa tttttcgtaa
    14161 aactcacccc ataacagttc tgaaagctta tcccagcgaa caacgtagcc cgtcagcgta
    14221 ccggctccgc tggtggtgat ttccgatgac cggatttcca tactcttcat aattttttca
    14281 cccataaaac actaaagggg ctttttagcc ccttctgtat gctgttaatc gtcgtcttcc
    14341 ggcagtgcca gaatcttgat cgcgttcgaa tccaccacgc caccgcctaa atatttttgc
    14401 gtgaaaattt tgatgaatcc tggttctgtc aggttgtccg gtctggtgcg aacacctgtt
    14461 tcgtgatcaa caatgtagta accgcgtttg aagtcaccca gggcaataac gttatcaggc
    14521 ataaactcca gatattcgac cggaaggccc agcaacgtat caggatcacc cgcctgtaaa
    14581 cggtcgcgcc agatataatc accgttcgcg ttcttcacct tctgaagttt tgccgccgtc
    14641 gtggagttaa ccactcagac cgcgtttttg cggtattttt tacgtaatgc aaatttcagg
    14701 tcgatcagcg ggtccgcaga tgtccacgcc agagattcgg aaggtttaat tacctgcaac
    14761 gtaccaaaat cacgctcttt gtcgttcttc tctgcacggg gtacggataa aaaacctttt
    14821 gcttttttgt caccgtcgcc cacaaccaga tcgctttctt cggtttccgt gaaggtgtcg
    14881 ccaatctcac ccgtcagcca tgaaaggatg tccacatcgg aaaaatccac gatttcctgt
    14941 gtggtgcgcg ggtacgcata gaccggatac agcttaatgc tcacctcgtt aatctgcggg
    15001 gtgctggtct gttcgcgtgc cttaccctct tcaccgtggt taacggtcgc accgccagcg
    15061 gaaacaagct gcttaaactc gttgctgctg attttcttca cggtacagat gcgacgcatg
    15121 gtggattcat ccgccagcat tcgcatgatt tcggtgttca gttcagggat aacggtataa
    15181 ccaccatcag cgggaacgcc tgtacttaat gcgcgggttt caccggtcag aatgtagtta
    15241 cgtagttctg cggggtcagt ggtctggctg cttttacctg gcttgctgcg ctcttcgtct
    15301 gcaatggctt caaggcggga aatgtcttta tcgagggatt cagctttagc gcgtaattcg
    15361 tcaaattttg cgccctcagc atcgttaaga ctgcggtttt ctttttcagc gttctccagc
    15421 atgtcgcgca tctgattttt aatggcggtt ttctgctggc gtaattcgat tattctctgc
    15481 ataaaaaaag tcctggggtt aagtaaggaa ctccaggacg cggcaaacac tcaaccgttt
    15541 ttcataagga aatcagtaat cgcaccgatc gttttcccgc ctggtaatga atattggcga
    15601 gcacattaac aggcgggaaa gtggccccag cgtcctggca ccacgggcga gaataatcat
    15661 gattcagttc gggtaaaata tgccgatccg ctcagtgaac aacgtggaac aaccacgaac
    15721 aaataattta caaaaaataa caaaaagccg gattgctccg gctgtatgtg ctgtatgggt
    15781 gctcagtctc tgatcccttt cagaaattct atcaatgcat ctgtctgttc aggatttact
    15841 gccagcattt caccggatag cgcgcaacga acaaaaccat gatgatcctt ttcaatcagc
    15901 gcccccgttt ccagaaatgc gcggtaatca gtgatattca tcgtctccat gttgtcagca
    15961 tggtatttat cacgctgttt aagtatctca tcaaatttca tcggcattgc gttttcctct
    16021 gttgtgtctg tttgtttcag atagtaacta tgccggaccg tgacgaaaag ccagtagtgc
    16081 tccataccgt ttcaaatgac gcaaaaaaag ccggattgct ccggctgcgt ggtgtggttc
    16141 tgatattcct actctgctaa tttgctcagc cccatgtctg cgtaagtgcg atttactgca
    16201 tttctcaggt ctgcgtagtt ctccgttggc ggctccggtg tgcccttgct gctctgtttt
    16261 accagcgggt cgcccaccag ctgcccgtaa gctgcaattt gtgctgtcat attgccacct
    16321 cagtgaagcg atccggcatt gttctttttc atctcttcca tcattttttc agtgagcatt
    16381 acgcactgaa accccatgtt tttgatgtct acgggtttca tgcctttgtc agtggatatc
    16441 acaggcagaa tatcctgata cacatcgcgg tcagggtaac taagcacaca aagctcccat
    16501 gatgccacgg gtgaggatcc ttcgtctgtt tcaacccacc agcctggtgt agctgcgctt
    16561 atttgcagaa tcgtgatccg tccgttgtgc ttgtaaaatt tttcaccaaa ttttggttcg
    16621 ctcatgattc cacctctccg gttttaacgt tgattgttgt tacctgttcc gcttcggcaa
    16681 tctcccgctc tgtcagcgtg gcaaaatttg ccgccgtcgt ggtcatgaat gcgcttatca
    16741 gttcgggatg tgctttcgcg tatccttccc ccgcgtggcg gtctatcgtt ctgattgcca
    16801 cctttaaagc gtgctctgtc atgtctaacg cgcgatattt cggttctgtt ctgtctctgc
    16861 gttttttgaa tgatttatta aatttccctg aagtgtgcat atttattttt accccctcgt
    16921 ttaaaaagtt ttgagttgtg cctccccttg tctaccttat ctaccttagt ggccctcatg
    16981 ccagtaatgg cgctgctttc agcggggtag agtgctttta tccactatct accccgtgtc
    17041 tacctccctg tctgattcag gtaaaatcag gtagagaggg tagatagtgg gtagacagta
    17101 aaaaaaggct atctacctaa cttaatgcac tgaattaaat gtatttttct ttactcaggt
    17161 agacagggta gacagcaatt acaaaaaatt ataaaaacgc gtcgcactcg tctgttgtta
    17221 ttgcgttagt ctgcgttact cccttaactt tccgcgtaat atattcatgt ccgtaaactt
    17281 tcgcggctgg cttcatagcc ttgccaaagt catttacgtt tagcggtttg ctcctgcctg
    17341 cgtacgccat aaacgccaga tagacgcggt aaaggctgtt tctggtcgtg tacttcactg
    17401 aatcgccacc gccgcccatc atcaggccgc gtgcttcctc cagaaaattc aggaactggc
    17461 aaaactcaat aaccggatcc gtctgttgct ttattgccag agcttcatca ccgtcacgct
    17521 gttccagtag taaagcccgt gctttctcag agtcggtaaa gttcgccagc aatcggcgga
    17581 taatgacagg gatttcagcc gcaatctttt ccggtagctc cctgtctttt tcggcctcac
    17641 taacgatatt gtcgaaacgg aaaatcacgc gacgacgtgc cacacctccg gcccgttcgg
    17701 tgaatatcat cgggttattg ttggtcgcca gcaccaccgc cctgattaca gccgtgaaac
    17761 gcttttcgta tttcgggtta atttccacgg ggtcaccgcc cgtgattttc ttgatgcccg
    17821 ttccttcgcc tgtatatttc ggctggtcag ccaggacgat aagacgactc ccgacaacct
    17881 gcgcacgtcc accagcatca tcaagcgatg tcatttcagc gcttaccgtg ttctgtttcc
    17941 ctgccagaag gctggctatg tgtgtgaatg tacttttacc gctcccgccg tctccggtgg
    18001 cctcaataaa catctgccag tcgtaccggt tcgccataat catgtacagc gcggcacata
    18061 tacgcatcat cttgcgctgg tcttttccgg ctgcgtgctc aagccattta tgaaagttta
    18121 gcgcgttatc gcggatgttc tcccctggtg ctggtggcgt gtactcaatg ccgttgtgcg
    18181 tggtgatcca gttctccggc gtgtgcgggg aaaattcccc cgttttcagg tcaagcgcac
    18241 cattggcgaa cggcagcaaa tcgccggacg gctcgcccat tggttcggca ataactttta
    18301 acgcttccac ggcgttattg attacgcgct tgctgaaagt ggccctgtgc tctgaataga
    18361 tcaccaccat ttcgcggcta agttccattg tgctgaccgg acaccatacc ccgccgcgcc
    18421 atacgtgaac gatttcactt tcctgatgca cacaaacgcc atcaaagcgc tcggcaagca
    18481 gctgcgcgcg ctcactgtct gccatttgtg ccagttgcgt atttttctgc tccggcatgg
    18541 taagccctgc ggcaatattt tcacgctcag cattcagata gcgccgccag ttttcacact
    18601 tctggacgtg cattccttcg gggtaaaaat ttgcgtcctg aatatctgcc gccgccagct
    18661 tctgaccaat ctttttggtc tccaccaaat ccagttctcc ggcctggtac agccttacgc
    18721 gtttttttcc atccggaaca attttcagtg catcaagttc ggcaagctga tttggcccaa
    18781 gccacacagg cggcacatta tcgccggatg cggggccatc ctgctcctgc cattgtttcg
    18841 cgtgcgacca ggcatcacta cccgcaaaga taattacctc tgtgtctttg tgttttattc
    18901 cgcgtggctg tttttttacg ttcggtgcca gtttcatttt ttacccctga atacgttaag
    18961 catctttttt atttcctgaa tattggcgcg tgctttctcc ctgctggttg gtttactgcg
    19021 gggcgctgcc tgtaccagcg tgaaatcacg ccggaattga taaacaggca tcacgcaatc
    19081 atattcgtaa ccttcacggc ggtaggtgat gcgccgttct gccacgcctt taatcgttac
    19141 catgccgccg tactggtcgc ggtaaatatc accgcgcgta aatttagggt gagtgttgcc
    19201 actggcagtt aagccagaat atttaagttt cattattttt attctccggt gtgttgttct
    19261 ttatatctgt cgtgcaatag atctatttct tgcagttcca ttattacagg ctcaagaagc
    19321 gttattaatg ccgtggcaat tcttgatttt tgtttgtcgc gttcattgtc gccaagtgtt
    19381 tcaagccata tgcgcaatat ttccagcatg ttttcactgt gagaaagtgc aagaaatgcg
    19441 cggtctattg tttcgtggta aatatcacgc atattaatcc ccgtccgtcg tttttcttaa
    19501 aataacctct gtcacgaaat cagcataatc ggcggcgata ttcagtacat caagcccgtt
    19561 gatttatatt ctctcgtgga aagtaagaaa aaagccgctc taataagttc tggcattgac
    19621 gaaagcgcat cagccgcatc atcaggaacg ccggaaaatt cctgtttcag ggaattaaaa
    19681 cgatcatcac gcataacccc ccccattttc acaatcggca acaagaatat ttttagcgtc
    19741 attcagtgac cgcgttgcga tgtatcggat acattccagg gcgaactgtg tccggtcttt
    19801 ctgttcttct ccctgcgcaa gctctgccgt gcattcaata tcaataagcg cgtgcatcag
    19861 cgtagtgagt gcggcggctg ctgcgttcgg tgtggtttta ttgtgcatag gtttccccct
    19921 gacgaatacg ggcggcgaat acagcaacac aaccggacgg gcaacggcta cgcgcttcgc
    19981 gttccgtcca agcggttacg tggatgattt gggattctcc ggcactcagt gccagaaaac
    20041 gccacacaaa ggccgtttgt gtgtgtacaa ggtgtggtat atgatttacg gcaaccataa
    20101 cggctcctag tttacgttgt tggttagaag ccccgttact gctcctacag tgcggggctt
    20161 tgctttttgt gttgattgtg tgtaatgtgt cactacacat aaatacatta cctgaggtgt
    20221 aattaacgtg tcaacacaca aaaacgaaag gcgtggtaat cctccgttcc agttccgttt
    20281 agatcctgac ttacgggaaa agatggaaaa ggcacaacaa caggacggcg atgattctct
    20341 tgctgcctgg ataaagcgaa taatccgcaa agaattacag caacgcggca tagaacccaa
    20401 aggataaaaa ctatcagcgc cgtggtgtga ggtactacgg cgcattgctt tacaggcgca
    20461 cacaatgacc aacgaagaat caaccgaaaa aacatcacct gcacgaaaaa gacgacgcag
    20521 aaagaaagag catgaatcag aacgattcgc gccgtgttct tttgcccttg agaagtacct
    20581 taaagaatac tcatcaaagg agagaacttc gcagttatgg caacgcactg aaccagactg
    20641 attgcattgc ccaccagcct gatagcggct atcatccccg tgcttatgtt tgggatcaca
    20701 cacacaaacg gcgcagcggg ttatctgttc agaaaggcgg ctccatttcg gggccgcttt
    20761 ttttatgcct gaaaaacccc aattttgtgg ttttccagtt tcaccagggc gaacgaatcc
    20821 ccgcccacgt tctggcgtat attcaatctt catggttata gctctgtgtt cagatgattg
    20881 atgtgtggcg gctgcgtgcc gccagcgtga ttaatgaact gccttgcagc tatccttcca
    20941 ggccagaacc tcggataaag accagccaac ggaacgaccg ccaagtttac gacgtgatgg
    21001 gaattgtccg gccttttcca ggcggtagcg gcatgagcgg ctaaggcctg ttagcttttc
    21061 gcattctttt tcacgtataa accgatcagt gcttaacact attgccccct ttcgtttctt
    21121 aaagagttat ttcgtgttct attgcgttgg gatgtgtctg attgtgtcag gatgattcag
    21181 agttggcaaa tgttgaggcc gtatggttta cagaaagagg aataatcagg ataaaatcct
    21241 ttaaattcat gttaatacaa aggcataaaa tattgtttca tgcctttttt ctcgctattt
    21301 aaagagtgat tcgctagtgt ataaaaaatc agtaacacat taaaaatcag ctacttataa
    21361 atctgtacac tttttcacct cttgttcgta gttgttccgc attgttgctc attgttgcac
    21421 gttgtatctg ttcgcatatc cagtatgcgc atactgaaaa aacacgaaaa aaattatttt
    21481 cttctggcta ctggtagcgt ggttacgttt tcatgtgttc ccgccagtat ccctaaccgc
    21541 tccgtccaca tatccagcgc attgcgttta gcatccagat aacgggaatg attataaact
    21601 cgctgcattc ctggcatctg gtggcctgta agctgctcca cgacgtgagg atccacgcct
    21661 aaatcgttca gcatcgttgt aaaggtgcgc cggatgtcat gtagtgacca gtgagggtga
    21721 tttagcctcc tgtgcgctaa tctgccgtac tgcgacacgc ttgtttcctg tttcacttcc
    21781 cccagcaata agcccgtgtg cctgttctgc tccaccagct gcgtgacgaa cggcagtatt
    21841 gcttccggta tgggccggaa tattgccacc ttcgttttgc tgtgttcttt cggcacggtc
    21901 cagagcattt cggtaaaatc ccactcgctg atctccgata acctcagttc taccgtccgg
    21961 catccgaaca caatcaggag gcggattaac gcgatgtagt agggggagaa tatttttttg
    22021 tccagtgcct gcaataattc gcccagttct ttggtgctta agacacgctc gcttatatcc
    22081 ggttttttcc caacgtccgc cacactcata tcatcaagaa cgttgctgat tgcatagcgc
    22141 cgcctccggc agaacttaag cgcctgtttg cacgtctgta gcaagaatcc ggcagtaaca
    22201 ggcgttcgct ttgccacctg gtcaaaacag gccagccagt gccgtagctc gcatttatcc
    22261 agcggcatag caccaatgtg ctgtattacg tgattattaa ggcgcttttt cagggcggca
    22321 taatccacgc ggttttcctt tgcgtacgac tcaagccagt aggtgagcgc atcgccaacc
    22381 gttaccggct ttaacgcttc ctgtacggtg taattaagct catgacgtgg atttttcccc
    22441 tctgccagcc atgcgcgaca ctgggcggct ttttccctgg ctgatttcag gctcagatca
    22501 ggataatttc ccagcttaat gcgttccggt ggtgcccccc ttcccgttcc ggccctgtaa
    22561 gtgaaatacc aggttaaaag gccactggtt gaatgcctga cgctcaggtt tccaccgtca
    22621 ttaagaaagg ctgttttttg ggcgggtgtg ccgttgattt tcctcagctg tgtatcgctc
    22681 agtttgttaa gtgctctgct cataattttg attccggtca tcacaattaa ctacaccatt
    22741 aactacaccg atcgttgcac aaagggctac aacgtgaaac aagctggaac aaggaaaatc
    22801 acaaatctgt ttataatcaa aaacataatg aacaatctga aacattatga aacggcaaaa
    22861 aacactaatt gataaaatat gtccatactt cacgcattac gttaagcatc cgttataatc
    22921 ggttgcagat accagcctgt ggatgcttaa catggaatac caactcactc ttaactggcc
    22981 cgattttctt gaacgtcact ggcagaaacg cccggtggtg ttaaaacgcg gctttaataa
    23041 ttttattgac ccgatctctc cagacgagtt ggcgggtctg gcgatggaaa gcgaagttga
    23101 cagtcgactg gtcagtcacc aggatggcaa atggcaggtc agccacggcc cgttcgaaag
    23161 ctacgatcat ctcggtgaaa ccaactggtc attactggta caggcagtaa accactggca
    23221 tgagccgacc gccgcgctga tgcgaccgtt ccgtgaacta ccggactggc gtattgatga
    23281 tctgatgatt tctttttctg tacctggcgg cggcgtcggc ccgcatctcg atcagtacga
    23341 cgtgtttatc attcagggta ccggacgtcg tcgctggcga gtgggcgaaa agctgcaaat
    23401 gaaacagcac tgcccacatc cggatctgtt acaggtcgat ccgttcgaag ccatcatcga
    23461 tgaagagctg gagcctggtg atattcttta tattccgcca ggattcccgc atgaaggcta
    23521 cgcgctggaa aatgcgatga actattccgt gggttttcgc gcgccaaata cgcgggaatt
    23581 aattagcgga tttgccgatt atgtgctgca acgtgaactg ggcggcaact actacagcga
    23641 tccggatgtt ccacctcgtg ctcatcctgc ggatgttctg ccgcaagaga tggataaact
    23701 gcgtgagatg atgctcgaat tgatcaacca gccggaacac tttaagcaat ggtttggcga
    23761 gtttatatcc cagtcacgtc atgaactgga tatcgcgccg ccggaaccgc cttatcagcc
    23821 agatgaaatc tacgatgcgc tgaaacaagg tgatgtgctg gtgcgcctgg gtggtctgcg
    23881 cgtattgcgc attggcgacg acgtgtatgc caatggtgag aagatcgatt ccccgcaccg
    23941 tccggcactg gatgcactcg ccagcaacat tgcgctgact gcggagaatt ttggcgatgc
    24001 gctggaagat ccgtcattcc tcgcgatgct cgcggcgctg gtcaatagcg ggtattggtt
    24061 cttcgaaggg taagtttgga tttaagccgg atgcagcatc acatgccgca tcccttttcg
    24121 cttacttccg ttgcgccgtt aactcggcaa tacggacgat cacctgcacc gctttttcca
    24181 taccttccag aatcacaaac tcatgcttac catgatagtt gtaaccgcca gtgaacaggt
    24241 tcgggcaagg taatcccata aacgacaact gcgcgccgtc ggtaccgccg cggatcggtt
    24301 tcagttccgg ttcaatatcg cagtcacgca tcgcctgctg ggcgatatcg agaatatgcg
    24361 gatgctcaac cactttctcg cgcatattgt agtaactgtc ttcaatcacc aattcaatgt
    24421 agcaatcagg atgtaaccct ttgcccactt ttttggcgat ctccatcatt ttacgtttac
    24481 gcgcttcaaa ctgtttacgg tcgaaatcac ggatgatgta gtgcatatcg gcccgttcaa
    24541 cggtgccttt catgctcgcc aggtgataga aaccttcata gccttctgtc atttccgggc
    24601 tttcatccgc cggaacttcc gcatgaatac gtgccgccag cgacagcgca tttaccatca
    24661 ctcctttcgc cgtgcccgga tgaacattgt taccgacaat ttttatattg accgatgcgg
    24721 cgttgaagtt ttcaaactcc agttcgccta cgccaccacc gtcaacagtg taagcccagc
    24781 gggcatcgaa ggcatcaaca tcaaaatgtt tcgccccttt gcccacttct tcatccgggg
    24841 taaaggcgac gcgaatatca ccatgcggaa tgtttttctg ttgcaatacc gccagcgcgg
    24901 tcatgatttc tgcaatacct gctttgtcat cagcacctaa caaggtttta ccatcggtgg
    24961 taatcaacgt ctgacccagt agctgatgca gcaccgggaa cataaccggt gataaaactt
    25021 catcgccgat acccagcgca atatcgccac cgcgatagtt ttcaacaatt tgcggattaa
    25081 catttttgcc gctgcaatcc ggtgaggtat ccacatgaga aataaagcca atcgccggga
    25141 tatcgccagg gacgttagcc ggtaacgtcg ccatcaaagt gcccttctca cttaaggtca
    25201 cattgataag ccccatttct tcgagctgct ctttcagcag atgcaataac ttccattggc
    25261 cttccgtgct gggaacctgt ctcacccctg cttttgattg ggtatccaga gacacgtagt
    25321 tcaaaaatcg ctcaagtagt ttatccatgt agtcaccctc actttttgtg acaacattat
    25381 taagaagcaa gaaaagacaa atattgcgtc aggtcacttt tacccctgca agcgggaata
    25441 tttatcagca ttaccttaat gaatataaag ctaagccagt aattcacaac aaagattggc
    25501 gattcaagcg gtttgccgta gaattaccgc cctcattagg agtcaccaag gtggttaacc
    25561 acaaaccccg catcggtaag ccatccgttg cgtttacatg ggacagagta aaaaattgaa
    25621 taaacaaccg agttcgcttt caccgctggt gcaattggcg ggaattcgca aatgctttga
    25681 tggtaaagag gtcattcccc agctggatct gactatcaac aatggcgagt tcctcacgct
    25741 gcttggccct tctggctgcg gtaaaacaac cgttcttcgc ctgattgcag gtctggaaac
    25801 tgttgattcc ggacgcatca tgctggataa cgaggacatc acccacgttc cggcggaaaa
    25861 ccgctatgtg aacactgttt tccaaagcta tgcacttttc ccccacatga ccgtgttcga
    25921 aaatgtggcc tttgggttgc gcatgcaaaa aacccccgct gctgaaatta cgccccgcgt
    25981 gatggaagcc ctgcggatgg tgcagctgga aaccttcgct caacgtaaac cgcatcagct
    26041 ctctggtggt caacagcaac gcgtcgccat tgctcgagcg gtggttaaca agcctcgtct
    26101 gttgttgctg gatgagtcgc tctcagcgct ggattacaaa ctgcgtaagc aaatgcagaa
    26161 cgagctgaaa gcgttacagc gtaagcttgg cattacattc gttttcgtga ctcacgacca
    26221 ggaagaagca ctcaccatgt cggacaggat tgtggtgatg cgcgatggtc gcattgagca
    26281 agacggcacg ccgcgtgaaa tctacgaaga gccgaaaaac ctgtttgttg ccgggttcat
    26341 tggcgaaatt aatatgttta acgccacggt gatcgagcga ctcgacgagc agcgcgtacg
    26401 cgccaacgtt gaaggccgcg aatgtaatat ctacgttaac ttcgccgttg aaccggggca
    26461 aaaactgcat gttctgctgc gcccggaaga cttacgtgtt gaagagatta acgacgacaa
    26521 ccacgctgaa gggctgattg gttacgttcg tgagcgtaac tacaaaggca tgacgctgga
    26581 gtcggttgtt gaactggaaa atggcaagat ggtgatggtc agcgaattct tcaatgaaga
    26641 cgatcctgac tttgaccact ctctcgacca aaaaatggcc attaattggg tagaaagctg
    26701 ggaggtcgta ctggctgatg aagaacacaa gtaagttcca gaatgtagtg attgtcacta
    26761 ttgtcggttg gcttgtgttg tttgtctttc tgcccaacct gatgatcatt ggcaccagct
    26821 ttttgacccg cgacgacgcc agtttcgtca aaatggtctt tacactggat aactacacgc
    26881 gtctgctcga tccgctctat tttgaagtgc tattgcactc gctgaatatg gcgctgatcg
    26941 ccaccctcgc ctgcctggtg ctgggctatc catttgcctg gtttctggcg aagctgccac
    27001 acaaggtgcg tccgctgctg ctgtttctgc tgattgttcc gttctggacc aactcattga
    27061 ttcgtatcta cgggctgaaa attttcctca gcaccaaagg ctatctcaac gagtttttgc
    27121 tctggctggg cgttatcgac acaccaatcc gtatcatgtt cacgcccagt gcggtgatta
    27181 tcggtctggt ttacattctg ctgccgttta tggtgatgcc gctgtactcc agtatcgaaa
    27241 aactggataa gccgttgctt gaggcggcgc gcgatctcgg tgccagcaag ttacagactt
    27301 ttatccgcat cattattcca ctgacgatgc cggggattat tgccggatgt ctgctggtga
    27361 tgctgccagc gatgggcctg ttctatgtgt ccgacctgat gggcggtgcg aaaaacctgc
    27421 tgatcggtaa cgtcatcaag gtacagttcc tcaatattcg tgactggccg tttggtgcag
    27481 ccaccagcat tacgctgact atcgtaatgg gcctgatgtt gctggtttac tggcgcgctt
    27541 ctcgtttgct gaataagaag gtggaactcg aatgatcggt cgactgcttc gcggcggttt
    27601 tatgaccgct atctacgcgt acctgtatat cccaatcatt attttgattg tgaactcctt
    27661 taacagctcg cgctttggca tcaactggca gggttttacc accaaatggt atagcctgct
    27721 gatgaacaac gacagcctgt tacaggcagc gcagcattca ctaacaatgg cggtgttttc
    27781 ggcgacgttt gctacgctta tcggttcact gacggcagtt gcgctgtacc gttatcgctt
    27841 tcgcggtaag ccgttcgtta gcggaatgct gtttgtggta atgatgtcac cagatatcgt
    27901 gatggcaatt tcgctgctgg tgctgtttat gctgctgggt attcagcttg gcttctggtc
    27961 gctgctgttc tcgcatatca ccttctgcct gccatttgtg gtggtgacgg tgtattcgcg
    28021 cctgaaaggt tttgacgtgc ggatgctgga agcggcgaaa gatctcggtg ccagcgaatt
    28081 taccattctg cgaaaaatca ttctgccact ggcaatgcca gcggtggcgg cgggctgggt
    28141 gttaagcttt accctgtcga tggacgatgt ggtagtttct tcattcgtca ccggaccaag
    28201 ttatgaaatt ctgccgttaa aaatttattc gatggtcaaa gtcggcgtat cgccggaagt
    28261 taacgcgctg gcaaccatat tactggtgct gtcgctggtg atggttattg ccagccagct
    28321 tattgctcgt gataaaacga aaggtaacac aggggacgtt aaatgaaaaa atggtcacgc
    28381 cacctgctcg cggcgggtgc tctggcactg ggcatgagcg ccgctcacgc cgatgacaac
    28441 aacacgctgt atttctacaa ctggaccgag tacgtgccgc caggactgct tgaacagttc
    28501 accaaagaaa ccggtattaa ggttatctat tcgacttacg agtcgaacga aaccatgtac
    28561 gccaagctga aaacgtacaa agacggtgcc tatgacctgg tggttccttc aacctattac
    28621 gtcgataaaa tgcgtaaaga ggggatgatc cagaagatcg acaagtcgaa gttaacgaac
    28681 ttcagcaatc tcgatccaga catgctcaac aagccttttg acccgaataa cgactactcc
    28741 attccgtata tctggggtgc gacggcgatt ggtgttaacg gtgatgcggt ggatccgaaa
    28801 tctgtcacca gctgggccga tctgtggaag ccagagtaca aaggcagcct gctgttgacc
    28861 gacgatgccc gtgaagtgtt ccagatggcg ctgcgtaagc tgggctactc cggtaacacc
    28921 accgatccga aagagattga agctgcatat aacgagctga aaaaactgat gccaaacgtc
    28981 gcagcgttta actccgataa cccggctaac ccgtacatgg aaggcgaagt taacctcggc
    29041 atgatctgga acggttctgc tttcgttgca cgccaggcgg gtacgcctat tgacgtggtg
    29101 tggccgaaag aaggcggcat attctggatg gacagcctgg cgatcccggc aaatgccaaa
    29161 aacaaagaag gcgcgctgaa attgatcaac ttcctgctgc gcccggatgt ggcaaaacag
    29221 gttgctgaaa ctatcggtta tccaacgcca aaccttgcgg cgcgtaagct gttaagtcca
    29281 gaagtggcaa acgataagac gctctacccg gatgctgaaa ccattaaaaa tggcgaatgg
    29341 cagaatgacg ttggcgcagc cagcagcatt tatgaagagt attatcagaa gctgaaagca
    29401 ggacgttaat tcagcaagcg ggcggtggta ttaccgcccg ttttttagtt agcaaggatg
    29461 tggctaatga gtcaggacag taaagtttta tcccgctctt tcgtcggtat tgtggtccgc
    29521 ttgtaggcct gataagcgta gcgcatcagg caatgcttcc tgctttataa tccctccagc
    29581 aacttttcaa caaattctgg caccacctgg cttgccgggc cgtaatattt ctcggcaaat
    29641 tcattaccaa cctgactcgg ttcaagattc agttcaacgg tgtgagcacc atgtagtttg
    29701 gcttcgtgta caaacccagc cgccggatag acatgcccgg aagtaccaat ggcaataaag
    29761 acatccgcca tcgacaacgc catataaatt tcatccatgc cgagtggcat ttcgccaaac
    29821 caaacgacgt gcgggcgcaa gggtgccggg aactggcaac aatggcattt atcttctggc
    29881 gtaacgtctc cggtccagtc gagaacctga ccactttgcg aacaacgaac tttcagcaat
    29941 tccccatgca tatgaatgac attggtatta cctgcgcgtt catgcaggtt gtctatattc
    30001 tgcgttacca gcaaaaagcg atcgccgaga gcgtcttgca gtttagccag cgcaagatgc
    30061 gcggcgttag gctgaatttc tggctgctgc agctgtcgac gacgggcgtt ataaaacgct
    30121 tgcaccagtt caggatcgcg atcgaaacct tccggcgttg ccacatcttc aacccgatgt
    30181 tcttcccaca ggccatctgc ggcgcgaaag gtacgaatac ctgattccgc agaaattcct
    30241 gcccctgtca gtacgagtac tcttggtttt tccattgctt ccggcaccac tttatctctg
    30301 aaaaaaatac gctgacgcaa acgctcgcgc aggcgacgtt tatttttacg aaaacgactt
    30361 aaccgatgac cccgacgcga cagcatagca acctctgttg tttaatcggt tagatgtagg
    30421 aaggccgcac cacgcattcc tcccgcatca ccgtggcgcg cgcgttcaat gcgcggaaca
    30481 cgagctacag gtaagagatg acaaggcaac ctgtccgcca gttgcgttgt gattgccggg
    30541 aaattcgata agccaccacc aatgacgacc aggtcagggt caacaatggt caggatattt
    30601 cccagagaaa ccgctaataa atccagataa cgctcaacgt gcgcccttgc ctgctcatcg
    30661 ccttgatcat aaagcgcaat aatttcggga gcctgcaacg gttgatgata atagtgttga
    30721 tacagccacg caaaaccgcg accagacaga taattttcaa tgcagccatg ctgaccacag
    30781 ccgcagcggc gtaacgggaa atccaacccc atcatggtta acgcatcaac cggcagacgc
    30841 atatggccaa actcgccggt aatgtagctt ttaccagtaa tcggtttgcc gttgaaaacc
    30901 agcccgccgc caacgccggt gccgagaatt aaccccatca ccagtggata ttgcgtaaat
    30961 tcatcatccc aggcttctga aagggcaaaa cagttggcat cgttatcaag gcgtacatcg
    31021 cgatcaagac gtgcgctcag gtcggcacgc agcggtttgc cgctggcagc ggggacatta
    31081 gcggcataca gcttaccatc ttctgtttcc ggcattcccg gaataccgat gccgacagaa
    31141 cctttacagc caaaacgttg gtcagcttca gctaccagct cacacactgc atctaaaaat
    31201 gcgtcatagc tgtcacgcgg tgtcggcacc cgcttttccc actgcaactg ccgaccgcta
    31261 tcaaacacgc caagcgcaat ttttgttcca ccaatatcaa acccgtaata cattgccgct
    31321 ccttgtttta atgtactgcc tttactggcc gctaaggact cgcgcagggt caatattact
    31381 ggcgcgccgc gccggatacc aacttgccaa aagactcagc aacaatgctg tgaccagtac
    31441 gtagaagacg tccagccagt gcaattccga tggcaggaag tcaataaaat aaatatcgct
    31501 ggagaggaac tgatgaccga tcagcttttc aatccactca ataatcgggg taagttgcag
    31561 tgaaacaact acgccgataa ccaccccaca caggctgccg aacagccctg ccagcaatcc
    31621 ataccagaca aagatggcgc gaattaaacc atctttcgcc cccagcgttc ttaatactgc
    31681 gatatcgcca ctcttgtctt tcaccgccat cactaaggtg gagacgatgt tgaaacaggc
    31741 cacgccaatc accagtacca tcgccagata cataatggcg cggatcatct ggatatcgcg
    31801 atacatatag ccgtaagtac caatccaact tttaatataa acatagctgt tggtcacttc
    31861 acccgcatcg cgtaccagct tattggcgtt gaaaacatcc gtcattttaa gggcaatacc
    31921 tgacacgctg gaacccatat caagatattg ttgggcatcc gccagcggga tcatggcaaa
    31981 actgtgatcg agttgaccac tcaactgcaa aataccggca atgtgcaaac gcacacgttt
    32041 tggctgcatc agtttatgct cgggattcga gttggggatc atgatcgaca cccaatcgcc
    32101 ctgcttcact ttcagcgcat ccgccacgcc tttgccgatg ataatttgct gttcgcccgc
    32161 tttaaaattg cgccacgcat ccccctgaac aaacgagggt aatgcgctca gacgctgttc
    32221 ctgttgtggg ttaacgccct tcacctggat tgcgcgcaga ttcgctccac tttccaccag
    32281 accggtgaaa ttgatatacg gcgcagcggc ggcaataccc ggcactttct gcacgttatc
    32341 cagtgcttcc tgccagttag tccatggctg atttaccgct tcgatttcgc catgcggcac
    32401 caccgccaga atgcggttat tcagttcgcg ttcaaagccg ttcatcgcgc ttaagccgac
    32461 gatcaatacc gccacgccga gggcaatgcc aatggtagaa atgacagaga ttagcgacac
    32521 catgccgccg cgtcgccgtc cgcgactaaa acgcaggcca atcaataacg ataaaggcat
    32581 cgccattact ccgcccccat caggctcagt tccgccgtca gacgtccatc acgcatttcc
    32641 agttggcggc tcatacgttt cgccagttgc aggtcgtgag tgaccaccag gaaagcggtg
    32701 ccctgcaagc gattcaactc cccaagcaac tggaagatgc tgtcggcgtt acgcgcatcg
    32761 aggttaccgg taggttcatc cgccagtacc agacgcggat tattgaccag cgcacgggca
    32821 atcgctacac gctggcgttc gccgccagaa agttcagatg ggcggtgatt cgcacgatgc
    32881 tccagcccca ccgcttttaa catctccagt gcacagctgt tgatttcagc gggctttttc
    32941 ttgccaatca gcagtggcat agccacgttt tccagggcag taaaatccgg cagcaggtgg
    33001 tgaaactgat aaataaagcc cagcttctgg ttgcgcagtt ctgctttcgc cgctgaagac
    33061 agtttgctca tcggctgacc gttaaagatc acatcgccgg aggttggcgt atccagcccg
    33121 cctaacaggt gcagcaaggt acttttaccg gaaccagagc taccgacaat tgccatcatt
    33181 tcaccttcgc ccacgctgaa actgacattg agcagcacat cggtttgcac actgccttcc
    33241 tgatagcgtt tgcacaggtt gtcgcattgc aacaggatct tattcataac gtaaagcctc
    33301 agcgggttgt gtggcggcag cgcgccatga agggtaaagc gtagacagca gcgcgatagc
    33361 catcgccacc agcgcaataa caatgacctg taaaggttcg atagccaccg gcagcgccgc
    33421 gccatcaagc aggacgccga ttatcggcat cagattattt aactggctgg caagcagtgc
    33481 gccaagcgcc gctccgagga ttgcaccgat aatcccggcg ctagcccctt gcaccataaa
    33541 gaccatcatg atttgtcgcg gagttaagcc ttgcgtttgc aggatcgcga cttcgccctg
    33601 cttctccatc accatcagcc ccagcgaggt aataatgtta aacgccgcaa cagcgacaat
    33661 caggctcagc agtaagccca tcatattttt ttccatgcgt acggcctgga acagctcgcc
    33721 tttacggtca cgccagtcct gccatttgct gccttcaggc agtttttgct gacttaaaga
    33781 gtcaactctc agcggctcat ccagccacaa acgccagccg gtaatattgg ctgccggata
    33841 acgcatcaga cgcgaggcat cctcaatatt caccagcatt tcatagccat cgacttcact
    33901 gttggcggcg aaagtgccaa tcacattgaa caggcgctga cttggaatac gccccatcgg
    33961 cgtgaactgg ctggcagatg gcaccatcac gcggatttga tcaccgcgat tgacgcccaa
    34021 ctgtgaggca agttgttcgc cgaggatgac attatatttc cccggctcga ggtcagtttg
    34081 tttcacattg accagatacg gcgttagtgg atctttttgc gccgggtcga taccgagcat
    34141 caccccgacc gccacgctgc gcgcgctttg cagtaccaca tcaccggtag taataggtgc
    34201 gacgcgatta acgccgtcca gtttgactgc cgtttccggg agttgctgcg ggttaagaga
    34261 gccatgctca gaagagagaa ttgcctgtgg catcaggcca aggatgttgt tttgcagctc
    34321 gcgctcaaag ccgttcatca ctgacaatac tgtgaccagc gccatcaccc cgagggtaat
    34381 gccgatggta gaaagccagg agacgaaacg accgaagcga tccactgcac gcccacgcat
    34441 gtaacgcagg ccaataaata gagcgacagg ttggtacatg aaatccgtct ggttgctgta
    34501 gcaaagacac gagtatataa gcgaaagcaa gatgggtaaa cgtctgaact gtaagtgatc
    34561 gtcagggcta atctgaaaag cccccgtaaa tcaatcggct aaattgccat attttatcat
    34621 caatcttgcc gcctgatctt aatctgaaaa atcatccaat acagacagat acccattaca
    34681 cctcgggtca taaccagcag gatcaaccgc atgtaaatta aaaaagagcg tgttgttctg
    34741 atgaaacaaa aagagctatg gattaaccag atcaaagggt tatgtatttg tctggtggtg
    34801 atttatcact cggtcattac cttttatccg catatgacca cttttcagca tccgttatcg
    34861 gaagtcctga gcaaatgctg gatctatttc aatctttacc ttgccccctt tcgtatgccg
    34921 gtttttttct ttatatccgg ctatttgatt cgccgctata tcgacagcgt accgtgggga
    34981 aattgtctcg ataaacggat ctggaacatc ttctgggtgc tggcgctttg gggcgtggtg
    35041 cagtggctgg cgttaagtgc gctgaaccag tggctggcac ctgagcgcga tttaagcaat
    35101 gcctccaatg ccgcttatgc cgattccacc ggtgagttcc tgcacgggat gatcaccgcc
    35161 agcaccagct tgtggtatct gtatgcgtta attgtctatt tcgtggtatg taaaattttt
    35221 agccgtctgg cgctgccact attcgccttg tttgtactgc tgagtgtggc ggttaatttc
    35281 gttcccacgc cgtggtgggg aatgaacagt gtgatacgca atttgcctta ttacagcctt
    35341 ggcgcatggt ttggcgcaac aataatgacc tgtgttaaag aggtgccgtt gcgccgccat
    35401 ctgctgatgg cttctttgct taccgttctg gcggtcggtg cctggttgtt tactatctcg
    35461 ctgctgttgt cgctggtatc gattgtggtg atcatgaagc tgttttatca gtacgagcaa
    35521 cgattcggta tgcgctccac cagcctgctg aatgtgattg gctccaacac cattgctatc
    35581 tacaccaccc atcgcattct ggttgaaata ttcagcttaa ctctgcttgc gcaaatgaac
    35641 gcagcacgct ggtcgccgca agtcgaactg acactcctgc tggtttatcc ctttgtcagt
    35701 ttgttcatct gtactgttgc gggcttgctg gtaagaaaac tttcacagcg tgcattcagc
    35761 gatctgttgt tctccccgcc ttcactgccc gctgccgtca gttactctcg ttaaaccatc
    35821 accccagccg gggcgattca atttgctaaa ccatgtcgat tacggataat gaacagcatt
    35881 gcttatcaga gatatgcccc catatgttga ggcatatcct aacgagaatc tgacaaccgt
    35941 tatgcctgaa caatatcgtt atacgctgcc cgtcaaagcg ggtgagcagc gtctgctggg
    36001 cgagttaacc ggcgcagcct gtgcaacgct ggtagcggaa attgccgaac gtcacgccgg
    36061 tccggtggta ctcattgcac cagatatgca aaatgctctg cgtttgcatg atgaaatcag
    36121 ccagttcacc gatcagatgg tgatgaatct ggcggactgg gaaactcttc cctacgacag
    36181 tttttcgcct catcaggaca ttatctcctc gcgcctttcc accctttacc agctaccgac
    36241 gatgcagcgt ggcatactga ttgttccggt gaatacgctt atgcagcgcg tttgcccaca
    36301 cagttttctc cacggtcatg cgctggtgat gaaaaaaggt cagcgcctgt cacgagatgc
    36361 attacgaacc caactggaca gcgccggtta tcgccatgtt gaccaggtga tggagcacgg
    36421 cgaatacgcc acgcgcggcg cgttgctgga tctcttcccg atggggagtg agctgcctta
    36481 tcgtcttgat ttctttgatg atgaaatcga cagcctgcgg gtgtttgacg tcgacagcca
    36541 gcgcacgctg gaggaagtag aagcgatcaa tctgctgccc gcgcacgaat ttccgaccga
    36601 taaagcggca attgaactgt tccgcagcca gtggcgcgat accttcgaag tgaagcgcga
    36661 tccggaacat atttaccagc aagtgagtaa aggcacatta cctgctggga tcgagtactg
    36721 gcagccattg ttcttcagcg aaccactgcc accgctgttc agttatttcc ctgccaatac
    36781 cttgctcgtg aatactggcg atctggaaac cagtgccgaa cgtttccagg ctgacacgct
    36841 ggcgcgtttt gagaatcgcg gcgtcgatcc gatgcgcccg ctgttgccac cacaatcgct
    36901 ctggctgcgg gtggacgagc tcttctcaga gctgaaaaac tggccccgag tgcagctaaa
    36961 aactgaacat ttaccgacaa aagccgcgaa tgccaattta ggtttccaga aactgccaga
    37021 cctggccgtt caggcgcaac aaaaagcgcc gctggatgcg ctgcgtaagt tcctcgagac
    37081 tttcgacggt ccggtggtgt tctcggtaga aagtgaaggt cgccgtgaag cgctgggtga
    37141 actgctcgca cgaattaaaa ttgctccgca acgcattatg cgtcttgatg aagccagcga
    37201 ccgtgggcgt tatctgatga ttggcgctgc cgaacatggt tttgtcgata aggcgcgtaa
    37261 tctggcgctg atctgcgaaa gcgatctgct cggtgaacgt gttgcccgcc gtcgtcagga
    37321 ttctcgccgc accatcaacc ccgatacact gatccgtaac cttgcggagc tgcatattgg
    37381 tcagccggtg gtccatctgg agcacggcgt cggtcgctat gccggaatga ccacgctgga
    37441 agctggcggc attactggcg agtacctgat gctcacctat gccaacgacg ccaaactgta
    37501 tgttccggtg tcgtcactgc atctgattag ccgttacgca ggtggcgcgg aagaaaacgc
    37561 cccgctgcat aaacttggcg gcgatgcgtg gtcacgcgcg cggcagaaag cggcggaaaa
    37621 agtgcgtgat gtggcggcgg aattgctgga tatctacgcg caacgcgccg ccaaagaggg
    37681 cttcgcgttt aaacacgatc gtgagcagta tcagttattc tgcgacagct tcccgtttga
    37741 aaccacgccg gatcaggcgc aggccattaa tgcggtactt agcgacatgt gtcagccgct
    37801 ggcaatggat cgtctggtgt gcggcgatgt tggctttggt aaaacagaag tggcgatgcg
    37861 cgccgctttc ctggcagtag ataaccacaa gcaggtagcg gtgttggtgc ctaccaccct
    37921 tcttgcgcag cagcattacg acaacttccg cgaccgtttc gccaactggc cggtacgtat
    37981 cgaaatgatc tcccgtttcc gcagcgccaa agagcagacg caaatccttg cggaagtggc
    38041 ggaagggaaa atcgatattc tgatcggtac gcacaaactg ctgcaaagtg acgtcaagtt
    38101 taaagattta ggcctgctga ttgtcgatga agaacaccgc ttcggggtgc gtcataaaga
    38161 gcgcattaaa gcgatgcgcg cgaacgtgga tattctgacg cttactgcaa cgccgatccc
    38221 acgtacgctg aatatggcaa tgagcggaat gcgtgacctg tcgattatcg ccacgccgcc
    38281 cgcccgtcgt ctggcagtta aaacctttgt ccgtgagtat gacagcctgg tggtccggga
    38341 ggcgatcctg cgtgaaattt tgcgcggagg acaggtttat tatctctaca atgatgtgga
    38401 aaacattcag aaagccgccg aacggctggc agaactggtg cctgaagcac ggattgccat
    38461 cggtcacggg cagatgcgcg agcgcgaact ggaacgggtg atgaatgatt tccatcatca
    38521 acgtttcaac gtgctggttt gtaccaccat tatcgaaacc gggatcgaca tcccgacagc
    38581 caacaccatt atcattgaac gtgcggatca cttcggtctg gcgcagctgc accagttacg
    38641 cggtcgcgtc ggacgttcac atcatcaggc atatgcatgg ctgctgacgc cgcatccaaa
    38701 agcgatgact accgatgcac aaaaacgtct tgaagcgatt gcctcgctgg aagatctcgg
    38761 ggcaggtttt gcgctggcaa cgcacgatct ggagattcgc ggcgcgggtg aactgcttgg
    38821 cgaagaacaa agcggctcaa tggaaaccat cggtttctcg ctgtatatgg agttgctgga
    38881 aaacgccgtc gatgcactga aagccggacg cgagccgtcg ctggaagatc tcaccagcca
    38941 gcaaacagaa gtcgagctgc ggatgccgtc gctattgcca gatgatttca tccctgacgt
    39001 gaacacgcgt ctgtcgttct acaaacgtat tgccagcgcc aaaacggaaa acgaactgga
    39061 agagatcaaa gtcgagctta tcgatcgctt cggcctgctg ccagatccgg cgcgtaccct
    39121 gctggatatt gcccgtctgc gccagcaagc gcagaaactg gggatcagga agctggaagg
    39181 taatgagaaa ggcggggtga tcgaatttgc cgagaagaat cacgttaatc cggcctggtt
    39241 gattggtttg ctgcaaaaac agccgcagca ttaccgcctt gatggtccga cgcgcctgaa
    39301 atttattcag gatttgagtg agcggaaaac gcgtatcgaa tgggtacgcc agtttatgcg
    39361 tgaactggaa gagaacgcga tcgcttaatg taaatcgggg gcgtttctgc gcccccatct
    39421 gcaacattta caaattcttt gcactttcct gcactatccg acactgtcac catccataat
    39481 tcaggcttat ctgtttatta caataacctt atatttatta tgtatttttg gtgatgatca
    39541 aaacgcgttt ttctcgctgg ctaacgtttt ttacgttcgc cgctgccgtg gcgctggcgc
    39601 taccggcaaa agccaacacc tggccgctgc cgccagcgag cagtcgtctg gttggcgaaa
    39661 acaaatttca tgtggtggaa aatgacggtg gttctctgga agccatcgcc aaaaaatata
    39721 acgtcggctt tctcgctctg ttacaagcta accccggcgt tgatccttac gtaccgcgcg
    39781 cgggcagcgt gttaacgatc ccgttgcaaa ccctacttcc agacgcgccg cgcgaaggca
    39841 ttgtgatcaa cattgcggag ctgcgtctct attactaccc gccgggtaaa aattcggtaa
    39901 ccgtgtatcc aataggtatt ggtcagttag gtggtgacac gctgacaccg acaatggtga
    39961 ccaccgtttc agacaaacgt gcaaacccaa cctggacgcc aacggcaaac atccgcgccc
    40021 gttataaagc acagggaatt gagttgcctg cggtagtgcc ggctggaccg gataacccaa
    40081 tgggccatca tgcgattcgt ctggcggcct atggcggcgt ttatttgctt cacggtacga
    40141 acgccgattt cggcattggc atgcgggtaa gttctggctg tattcgtctg cgggatgacg
    40201 atatcaaaac actctttagc caggtcaccc caggcaccaa agtgaatatc atcaacactc
    40261 cgataaaagt ctctgccgaa ccaaacggtg cgcgtctggt tgaagtacat cagccgctgt
    40321 cagagaagat tgatgacgat ccgcagctgc tgccaattac gctgaatagc gcaatgcaat
    40381 catttaaaga tgcagcacaa actgacgctg aagtgatgca acatgtgatg gatgtccgtt
    40441 ccgggatgcc ggtggatgtc cgccgtcatc aagtgagccc acaaactctg taaatcctgg
    40501 ataaaaaaaa tccccgcgac acggcatgtc gcggggaaaa aatcagcagt agcaggcatt
    40561 aatgagggtt aatgcttatt tataaattac tgctgttcca tggagggtat tcggaccggt
    40621 tacagaagta atacggaaag attttgcgcc catctcatcc gctttttgcg ccagctgctc
    40681 ttccagcgat cccagatttg tccccgcgtt agcactgata gtaccgactt tttgttggcc
    40741 ttctggcgtt gactgaactt cgacagccgc aaagttggca aatgacatgg agcttaaaat
    40801 cgccgcagcg atgaggtttt tacgtttttc ataatagtgg ccttatgcag atgaatgacg
    40861 tcgcaagaaa ttacttaacg atcgataact aaatgataga tgtgatctgg atcacataca
    40921 agatattttt tataacaatc attaattaat ttaaaaatct ttaaatttca gtcaaataga
    40981 aagcaattat ttttatggga tctggactgg tgaacggagg tgattatttt tataataacc
    41041 tttcattcac agtcaccagg tacaacggca tcatggcaac tgactcaaca caatgtgtaa
    41101 aaaaaagccg tggccgccca aaagtgttcg acagggatgc cgcgcttgat aaggccatga
    41161 aattgttctg gcaacacggt tatgaagcga cttctcttgc ggacctcgtc gaagcaaccg
    41221 gagccaaagc gcccacgcta tacgcggaat ttaccaacaa agaggggtta tttcgtgccg
    41281 ttctcgaccg ctatatcgat cgttttgccg ctaagcatga agcacagctg ttttgtgaag
    41341 agaaaagcgt ggagtctgcg ctggctgact attttgctgc catcgccaac tgctttacca
    41401 gcaaagacac cccggcaggc tgcttcatga tcaacaactg caccaccctc tccccagatt
    41461 caggagatat agccaatacg ttgaaatcac gccatgcgat gcaagagcgc actttgcagc
    41521 agtttttatg tcaacgacaa gcgcgcgggg aaatcccgac ccactgtgac gtgacacatc
    41581 tggcagaatt ccttaattgt attattcagg ggatgtcgat cagcgcacgc gaaggtgcat
    41641 cgctggaaaa actgatgcag attgccggaa cgactttgcg tttatggccc gaactggtga
    41701 aataagtcgc aaaaaaggcc cctcagcctg agcgaatgag gggccaaatt gcagagtaca
    41761 gccttgttat actttgttat tcaaaatcag ttgcccgtta ctatctagcg ggatctgcgt
    41821 acccggatcg cggtccatgc ggattttgcc ctgctgatcg ccaatcttat aggtcacatc
    41881 ataaccgagc attttttctg acttgtcata caccgtttta caacgctgtt gcgtagtcgt
    41941 gtaagtatcg ctttcctgga gagagccctg gatctggtta ccggcatatc caccacccag
    42001 cgcccccaca acagtggcga catctttacc gcgaccacca ccaaactgat gccctatcac
    42061 gccaccagca acagcgccga gcaccgaccc ggtaatgcga ttttcatcct gcaccggtcg
    42121 acgatgggtc actgtgacgt tgcgacactc ctgacgcggt gttttaaccg tttccttgat
    42181 tggggttgca gaaacaacct gagcgtattg cgggccccgt tcaaacacgt tcagactggc
    42241 cactgccgct acgcccaacg cagctgcgac accaatcccg atacccgcca acattgattt
    42301 attcacggga acacctcctt ctttgcagtt atgccaatca tgcaagcgaa ggagaaaagc
    42361 agctatcaga cagaagatga aaagcgcggg aatgactgga aatacttaag gattcggagg
    42421 agtctgaagg aatgcctgat gcgacgctta acgcgtctta tcaggcctac cgctcgtacc
    42481 atttgtccag ggcggataag gcgtttatac cacatccgcc agtgtacgtc gattaatgca
    42541 acttcaaacg cggacggata acgcggttaa tactccccac cagcatcatt aatccggttt
    42601 taaagtaacc atgcagcgca atctgatgca ttcggtatag cgagatatat acaaagcgcg
    42661 caattcgtcc ttcaatcatc attgagccgc gcgtcaggtt acccatcagg ctaccaacgg
    42721 tggagaagtt cgacagcgat accagcgaac catgatcttt atactgataa tttttcagcg
    42781 gcttaccgtt catctgcgcc agaatgttgt tcattgcgca agtcgccatc tggtgtgcag
    42841 cctgagcacg cggcggaaca aagccccctt ccggacgcgg gcatgacgcg cagtcgccaa
    42901 tagcgtaaat gtctggatcg cgagtggttt gcagcgtcgg ttccaccacc agctggttga
    42961 tacggttagt ttcaagacca ccgatatctt tcaggaagtc tggcgctttg atcccggctg
    43021 cccataccat cagatcagcc tcaatatatt cgccatcttt agtgtgcagg ccgccttcat
    43081 cagcactggt gaccatggtt tgcgtcagca cgcgaacgcc aagtttcgtt agctcgttgt
    43141 gggccgcagc agagatacgt ggcggtaatg caggcaaaat acgttctccc gcttctacca
    43201 gcgttacgtt cagggcttcg ttggtcaggc ctttgtaacc gtagctgtgc agttgcttga
    43261 tcgcgttgtg caattcagcg gagagttcta cacccgtcgc gccgccgccg acaatcgcaa
    43321 tgttcacttt gccatttgcg cccaggttgg cagagtattt caggaacaga ttcagcatct
    43381 cctggtggaa gcgacgcgcc tggtgcgggt tatcgaggaa aatgcagttc tctttgacgc
    43441 ctggcgtatt gaaatcgtta gaggtgctac ccagcgccat gaccagggtg tcataggcga
    43501 ttttacgttc cggaaccaac agttcacctt tctcgtcgcg tagttctgca atagtgattg
    43561 ttttcgcttc acgatcaata tcaatgacgg aacccaactg gaactggaaa ccatgattgc
    43621 gcgcatgggc cagatagctc aacgcatcga cgccttcatc aagcgagcca gtcgccactt
    43681 cgtgcagcag tggtttccac aggtggctgt ggttacgatc gaccagcgta attttggctt
    43741 ttttcttacg tcccagctta tgccccagct gtgttgccat ttccagccca ccagcaccgc
    43801 cgccgacaat cacaatcttt ttcaatggcg tagtcaacgt gcccccctta aatttattaa
    43861 ccaattgtta attaatggtt attaacatag ctcttaatta acaacaggtt acagcaatga
    43921 aaatgttctt cgctattgag aataacatga atggtgcatt ggtcatacca aaattgatgt
    43981 taatcaagtt ttgttgctta aaagataggc aactttaccg ggggagatca aaaaatagag
    44041 tttattgttt attatttgag caaattcgga agggtaatat agggtgaagc gtatatcgag
    44101 ggcgtttggt cgtcctgata agatgcgcag catcgcatca ggcattgatg ctcgactgtc
    44161 ggatgcggcg tggacgcctt atccgactta cagaattcgg agcgcatcag gtaccggcaa
    44221 ggttacctga tgcgacgagg gcatttaccc gagggttttg aacgctttaa tgcgctgtaa
    44281 atgcggggag atattcttga atttgtgcgt ctgctcttcg tcccagacaa tctcgtaata
    44341 atgatgcaac tcttcagatg tccgctggct gttaagtgct tcatcattac gcgacaaaat
    44401 aaccagacaa cgatcgcgat tcttctcgcg gaagttggtc acacacttag tggcaatatc
    44461 ggcatactct tccgggcgat caatcttgcc ttccatgttc tcataaggga acaaattagg
    44521 gttgaagatc acctggcgga tatcgcaaag aaaaccaatc cgttccgccc agtatccgcc
    44581 caggccaacg ccgcaaatta gcggacgctc gtcaacgttc agttgcaaca ttttgtccac
    44641 ttctttaagc agatgctgca tatcatgttt cggatgccgc gtactgtagc ttatcaagcg
    44701 tacatccggg tcaataaact gcaattgtaa gactttctcg tggttacccg gactgttaga
    44761 gtcaaaaccg tgtaaataga taatcatcgc ttcctcacat aagccagggt tagtgaccta
    44821 ctttctcttc ctgccagcgt tcatgtaact gattcagacg ggtgctgatc gctttccagc
    44881 gagccgagtc catcagttcc tgtcgcgaaa atgaaccttt atgatacaaa cgtgtaacac
    44941 gttctgcctt gatcggtgac agattatcta acacgctgac ggccccttta cgattattgc
    45001 agaccaggat catatcgcaa cccgcatcca gtgatgcctg cccgcgttcg gcataactgc
    45061 ccataatcgc ggcaccttcc atcgataaat cgtcagagaa aatcacgccg tcaaacccca
    45121 gttcctgacg caaaacggtt ttcagccagt agggagaacc gctcgccgga cgcggatcaa
    45181 catcactgta gatcacatgc gcaggcataa tggcgtcgag tttattttcg cggattaagg
    45241 aactgaagac cgacatatct ttagcgcgaa tctccgcttg tggacgtgga tcgcacggtg
    45301 tttctttgtg tgagtctgcc gttactgcac cgtgtcctgg gaagtgtttc ccggtcgttt
    45361 tcattccggc ttcatgcata ccatcaataa accggctggc aattgccagg gctttttgtg
    45421 gatcggcatg ataagaacgc tcgccaatcg ccgcgctgat atgcccgaca tccagcactg
    45481 gcgcaaagct gatatcaata tccatagcga tcatttcgct ggccatcagc caacccgcct
    45541 cttgcgccag tttgccgccc tcttccattc ccaacagcgc agcaaaggat tgtgctgccg
    45601 gtaagcgggt aaaaccttcg cgaaaacgct gcacgcgtcc accttcctga tctaccgcca
    45661 ccaccagatg attgcgcgat gctgcgcgga tctggcgcac cagttcacgt aactgggcag
    45721 gatcatgata gttacgcgta aagagaatca gccctcccac cagcggatgc gccagtattt
    45781 cacgctcttc cgcgtccagt tcgtaacctt cgacatccaa cattactgga cccacactgc
    45841 tctccttatt gttttattaa tagctgccgc caggtgtcat cggccagcct gataaattgt
    45901 tgatcgccgg tttgtcgcca gcggtactca aaccaccctc ctttgagcat cagcagccag
    45961 ggaaaccatc gcctgacctg acgccataat tgcgccggat aaatcttcgc gcgagtggca
    46021 tagtcattga ccaattgccg gtgctgttca gtattttcca cccacaccgc cgccagttcc
    46081 agcgcgatat caccatctcc agcatactcc cagtcgatga gttttaaccc tgatgcgcta
    46141 tgcactaaat ttccggcgtg gacatccata tgcaatggac ttaagcgtaa aggtcgtggt
    46201 tcccgcgctt tgcgcagacg ttttaacatt cgcagccaac ccacagtccg ccgcgccgga
    46261 tcgctttgct gccagtacag ttccagtaag ggcaacagcg ttattcgcca gccaaaacgt
    46321 ggttgttgat gtagataata cagcaagcct gccagttcgt tggtatccgg caaatacgtt
    46381 tttaccgcac cgggcagata gtcgactacc atccagtcac ggagatataa atgcggcttc
    46441 ggtgcaatgc ttgcgggtag ttgtgataaa gcccgatact ggcgcaagaa cgcggactgc
    46501 ggcgcatcag gatcgtgcgg ctgacgcaca acaaaacgct ggccctgatg ttcaatgaga
    46561 aaactaccgc cactaagccc actattaaac gtcgtgacgg gatgaaactg cgggaaaaag
    46621 cgcgacagca attcgtcgcg cgtgatggga ttattgctgc gaaacggcac ctttacctga
    46681 ccagataatt tcgcccgtct gcaccagcat cagctgcatt tgtagggtcg gagcgttaac
    46741 gttgccagag gcgcaggagt acagcacgta atgagcgccg acattgcggg caatgcctat
    46801 ggctttacta cgggtgccta aactgtcctg cggcgacaaa cctaactgtt gcttagccat
    46861 cgacagttgc tgggcggaaa ccagggtaaa tttcccgtta ttagccagcg catttcgcag
    46921 cgtttcggtc gcttctgcgg cattcagcga accgttagta cggttgttaa cgctatcaac
    46981 cagcaggacg ctacccgcag tcaccccgtc agccccaagc atcttactga ccatcggctg
    47041 cattgcgcca ttccagtcat aatggcgaat atgcggcgca ggcggtgccg ttcgatcttc
    47101 gtgctcaatt gggcctggct gctgcgggat cgtcggcacc gagggcactg tggggacagg
    47161 ctgttgtggc tcggctggtt gttccggcgc tggtttcact tcttctaccg gtgcaggttc
    47221 acgttgcccc acacacccgg cgagaaacat cgccagcgcg gtaatcaagg cgtagcgact
    47281 catttttgtc atcaagattc acccctttac aaatatagat aaagtctgac tttgtgcgcc
    47341 cccagaaaat tggcgctgcc gtacagcgtt accgccgaat gtgcgggaat ggtaacgctg
    47401 cgtggccttt ccagaggatg catctccagc cctctggcgt cataccagta aaaacgataa
    47461 tgaacggtaa cgggttcttg cctttcgtta taaatcgttg aggatgctga aggttgaata
    47521 tcagacgtcg aaaggacggg cttttctgca ctgatgcccg cagccaataa agatgactcc
    47581 atcaccagcg attgctcatc attcaccgga atttccggat gtgaacgaca gcccaccagc
    47641 agtaacaaca ccagagacac cagcccaaag catccttttc tcatcgttac agacctttat
    47701 gcgccagcat tggtcccagc ggacggccac ccaacaagtg catatggatg tggtaaacct
    47761 cttgtccgcc atggcggttg gtattcatga tcagacgata gccatcttcg gcaatacctt
    47821 cttgctcagc aatttttgcc gctacggtga tcatgcgtcc cagcgcctgc tcatgctcag
    47881 ctgacacgtc gttcacagtc ggaatgagaa tattcggaat gatcagaata tgcgttggcg
    47941 cttgcggcga aatatcgcga aacgccgtta ccagatcatc ctggtagacg atatcggagg
    48001 ggatctcacg acgaataatt ttgctgaata tagtttcttc tgccacgaca ttttcctttt
    48061 tcaaatagcc atgcgttgcg ccatgctacg ccgcgccaca ctgcgagtat agagtatggt
    48121 cgagttaaac ctgctctttc aatcttaact cacgatttct taagcaaaaa atcgttttgc
    48181 tgccgtatat atcgccatta ttcccatttc tgcctgcgaa tgcgctgaaa agacattctc
    48241 tgtttggttt acacgacaaa tatgaatgcg tatatttctc atttgcattt gcatttgcag
    48301 caaaattatt cgcacactaa atataaccgc tcatcaccct cctccggcat gagcctgacg
    48361 acaacataaa ccaagagatt tcaaatgctt tcaacacaat ttaacaggga taatcaatat
    48421 caagtcatca ccaaaccgtc actacttgcc ggttgcatag cactggcact attaccttct
    48481 gccgcttttg ctgcaccagc cactgaagaa acggtgattg ttgagggttc agccacagct
    48541 ccagatgatg gcgaaaatga ttacagcgta acgtctacct ctgcgggtac caaaatgcag
    48601 atgactcaac atgatattcc tcagtcggtc actattgtta gccagcagcg gatggaagat
    48661 cagcagttac aaacgctggg cgaagtgatg gaaaacacgc tggggatcag caaaagtcag
    48721 gcggattccg atcgtgctct ttattattcc cgcggattcc agatcgataa ctatatggtt
    48781 gatggtatcc ccacctattt tgaatcgcgc tggaatctgg gcgacgcact ttctgatatg
    48841 gcactgtttg aacgcgtaga agtagtgcgt ggcgcgacag gactcatgac cgggacgggt
    48901 aatccatctg cggcaattaa tatggttcga aaacacgcga cctgtcgtga atttaaaggc
    48961 gatgtctcgg cggaatacgg tagctggaac aaagaacggt atgtggcgga tttacaaagc
    49021 ccactcaccg aagacggtaa aatccgcgcg cgaattgtcg gcggctacca gaataacgac
    49081 tcatggctgg accgctacaa tagtgaaaag accttcttct cgggcattgt cgatgctgat
    49141 ttaggcgatc ttacgacgct ttcagccggt tacgaatatc agcgcattga tgttaatagc
    49201 ccaacctggg gcggtttacc gcgctggaat actgatggca gcagcaacag ttacgatcgc
    49261 gcacgcagta ccgcacctga ctgggcgtac aacgataaag agatcaacaa ggtctttatg
    49321 accctgaagc agcggtttgc tgatacctgg caagcgacac tgaatgccac ccactctgaa
    49381 gtcgaatttg acagcaaaat gatgtatgtc gatgcctatg taaacaaagc ggatggtatg
    49441 ctggttgggc catacagtaa ttatggacct ggctttgatc atgtcggcgg caccggttgg
    49501 aacagtggca aacgtaaagt tgatgcgctg gatttgttcg ctgacggtag ttatgaattg
    49561 tttggtcgtc agcacaatct aatgtttggt ggcagttaca gcaaacaaaa caatcgttca
    49621 gttcatgggc caacatcttc ccggatgaaa ttggcagttt ctacaacttt aatggcaatt
    49681 tcccacaagc cgactggtca tcacagagcc tggcgcagga cgataccaca catatgaaat
    49741 cgttatatgc tgccactcgt gtcacccttg ccgatccgct gcatctgatc ctcggcgcac
    49801 gttataccaa ctggcgggtt gatacgctga cttacagcat ggagaaaaac cacaccacgc
    49861 cttacgctgg tctggtgttt gacatcaatg acaactggtc gacctacgcc agctatacct
    49921 ctattttcca gccgcaaaat gatcgtgaca gttcaggcaa atatctggct ccaatcaccg
    49981 gtaacaacta cgagctgggt ctgaaatcgg actggatgaa tagccgtctg accaccacgt
    50041 tagccatctt ccgtattgag caggataatg tcgctcagtc caccggtaca cctatccccg
    50101 gcagcaacgg cgaaaccgcc tataaagcgg tggatgggac agtcagtaaa ggggtggaat
    50161 ttgaactcaa cggcgcaatt accgacaact ggcagctgac atttggcgca acgcgctatc
    50221 ttgcagagga taacgaagga aacgccgtta atcctaatct gccacgcacc acggttaaaa
    50281 tgttcaccag ctatcggttg cctgtcatgc cagagttgac agtcggcggt ggtgttaact
    50341 ggcaaaatcg cgtgtatacc gacaccgtga caccgtatgg caccttccgc gccgagcaag
    50401 gtagctacgc gctggtggat ctcttcaccc gctaccaggt gacgaaaaac ttctcgttac
    50461 aggggaacgt caataacctg ttcgacaaaa cctacgatac caacatggaa ggttctatcg
    50521 tctacggcgc accgcgtaat ttcagcatta ccggcacgta tcaattctga ataacacctg
    50581 taaaaaaggc agccatctgg ctgccttagt ctccccaacg tcttacggat tagtggttac
    50641 ggatgtactc atccatctcg gttttcaggt tatcggattt agtaccgaaa atagcctgaa
    50701 caccagaacc agcaaccact acgcccgctg cgcccagttt cttcaggcca gcctgatcca
    50761 ctttagacac atcagcaacg ctgacgcgca gacgggtaat acatgcgtcg aggttagtaa
    50821 tgttttcttt accaccaaat gcagcaacca gagccggtgc catttcgctg gtacctgttg
    50881 cttttgcatc ttcagtcgcg tcttcacgac ccggcgtttt cagatccagt gctttaatca
    50941 gcacgcggaa gatggtgtag taaacaatcg cataaccgat accgacgatc ggtaacagcc
    51001 acagtttgct gctgttacca gacagaacga tgaagtcgat cagaccgtgc gagaacgacg
    51061 taccgtcacg catccccaga agaatacaga ttgggaatgc caggcctgcc agaatcgcgt
    51121 ggatgatgta caggatcggc gcaacgaaca tgaagaagaa ctcgatcggc tcggtgatac
    51181 cggtcaggaa cgaggtcagc gccgcggaga tcataatacc gcccactttc gcgcggtttt
    51241 ctggtttagc agagtgccag atagcaattg cggcagctgg cagaccgtac attttgaaca
    51301 ggaagccacc agacagttta cccgcagtcg ggtcacccgc catataacgc ggaatgtcgc
    51361 cgtggaaaac ctgacctgct gcgttggtgt attcaccaat ctgcatctgg aaaggtacgt
    51421 tccagatgtg gtgcagacca aacggtacca ggcaacgttc gatgaaaccg taaatgccaa
    51481 acgcaactac cgggttctgg taagcagccc actgagagaa ggtctggatt gcagaaccaa
    51541 tcggcggcca aatgaaggac agcacaacgc cagtaaagat ggcagccagg ccagaaatga
    51601 tcggcacaaa gcgtttaccg gcaaagaagc caagatactc aggcagctta atacggtaga
    51661 aacggttaaa catgtacgct gcaatcgcac cggagataat ccctccgagt acgccagtat
    51721 ccgccaagtg tttagaggcg atttcttcag caggtaaatg cagtaccagt ggcgcaacca
    51781 cggccatggt tttaaccatg atgccatagg caacaactgc ggccagcgcg gatacgccat
    51841 cgttattggt aaagccgagg gcgacaccga tcgcaaaaat cagtggcatg tttgcaaaga
    51901 cggaaccgcc tgcttctgcc ataacatgcg atacaacggc gggcagccag ctgaaattcg
    51961 cggaaccgac gcccagcaga atacctgcga taggtagtac ggataccggc agcatcagcg
    52021 atttaccgac cttttgcagg ttagcaaatg cattcttaaa cataattgag agtgctcctg
    52081 agtatgggtg ctttttttac gttctcacgc gtggcaaggg gggagagcct cgccgtgtac
    52141 agggcatcta agcgcccttt atttattaca cagagtaaaa taattcagtg ccaatttgtt
    52201 tgacggctat cacgtttcaa cttcagatca ttggagaagt tgcacatttt gacaaaacat
    52261 atgtcacaat atttcaaacg cggttattca ccgcccaatt ttcggcggtg aactttactc
    52321 gttttagcca ttaattacga gctttaaaaa aactcattca acggatggat tgaaggcggg
    52381 aagcgtcgat gtgaaacaga cgggcgaagt tatcggtggt tacctgcgcc agttcttcaa
    52441 cggcaacacc tttcaacaca gccatgtatt ctgcaacgtc acgaaccatc gcaggttgat
    52501 tctcttttcc tcgatgcggt accggcgcaa ggtaaggtga gtcagtttcc accagtaatc
    52561 gatccagggg gacataacgc gcagcatcgc gcagttgctc cgcattacgg aaggtcacaa
    52621 tgccggaaaa ggagatgtaa aatccgagat ccagtaattt acccgccgtt tctctgtcct
    52681 ctgtaaaaca gtgtagtacg ccaccgcaat ccgtcacttt ttcttcgcgc agaattgcca
    52741 gcgtatcggc acgggcgtca cgggtatgga cgataaccgg cttgttcagt tcacgaccaa
    52801 tctggatatg atggatgaag gactcttgct gacgtacttt agtttccggt gtgtaataat
    52861 aatccagccc ggtttcaccc agcgctacaa caccctcttc tgccgccaga cggcgtaaat
    52921 cttctacatc gtagggatca ttctggttga gtggatgcac gccacaagaa aatacgacgt
    52981 tgtcacgttc gcctaccaga tcccgcatat gtaaataacc cggtaatgtt gtggcgactg
    53041 ccagacaaaa tttcacatcg cgtgcggcgg ctttcgccag aacgtcatcc acgtccttat
    53101 gcaaagattc ataatccaga ccatcgagat ggcagtgtga gtcgactaaa aacatgatgt
    53161 ctctcttaaa gatgaggaac cggtaacaca acgcccggtt gcaggtaatg ctcaatacgc
    53221 agtaaaagat cggtgatgag aagctcgcgg ttgatgcctg tgacagacat taactgttca
    53281 cgaatgtggc aaacatcccc cagtatagcc tgcaggcacg agggagaaag atggtttgcc
    53341 agttcggcga ccaggcccgg cacatcaaca ttggtcacct gcgcagcgcc atgatggcgt
    53401 tttagcgcat ccatcagcaa cgttgccagc cagtgtaaac gcgccggagc ttgttcatga
    53461 ttaagggccg ccagcagcga ataccagtcg cccgatggca cgctatatgc caacgcctga
    53521 cacaatgttt cacgagcctg ccagttatct ccctgaaaca acgccagtgc cgcgccaggc
    53581 gaaccggcgc ttaagcgcaa tgcggcaagt aatgcatcct gtgacattgt cacttcgcgt
    53641 gaaagccaag tcacggcgta ctgttctgga ggcggcgcaa ggtaatgtaa ccgacaacga
    53701 ctacgtaatg ttgccagtaa acgttcaggc tcgcgggtag ccaggaaaaa ccaggtttct
    53761 gctggtggct cttcaagcgt tttcagcaat gcgttagccg cggcgtcggt cagtaaggca
    53821 gcatcggtta cccagacaac tttcgcacca cctaggcgtg cgtgctcatt cagcttttcg
    53881 gtgacctcac gtaccgcatc aatgcccagc gtatttttcc ctttttcggg agccagggtg
    53941 tagtaatcgg gatgcgtgcc agcctgcatc aactgacatc cacgacagtg accgcaactt
    54001 ttgtggccct gcggttgttg gcagagtaaa taacggctca gggcgtagat taaagcatca
    54061 tcgcccatgc ccggtaacgc ctgaatgagt agcgcatggt gacctcttcc ggcctgatag
    54121 ctggctacca gtttttcgaa atcaggtcgt aaccatggat accatctcat gcgtccaact
    54181 ccttcaccca gtgggtcacg gtagtgcgga ttgcatccat cacggcctcc agcggctggg
    54241 tggcatcaat ggtatgaata cttttatctt gtgcagccag ttccagatag cgggcgcggg
    54301 tgcgattaaa gaaatcgaaa gactcttgct caatacgatc cagctcgccg cgcgcacgcg
    54361 cgcgttttaa gccaacttcc ggggtaacat cgagatagag cgttaagtct gggcgaaaat
    54421 ccccgagaac agcatcacgc agtgttgcca gcatatgttg gtcaatacca cgtcctccgc
    54481 cctgatacgc ctgagtggaa agatcgtggc gatcgccaat cacccaggtg ccgttagcca
    54541 gcgcaggttt gatgaccgtt tctaccagtt gaacgcgcgc ggcataaaac atcagaactt
    54601 cggctttatc ggtaatgact tcatcgccta ccgatttgat atccagcacc aggcttctta
    54661 acttttcggc aagttgcgta ccgccaggtt cccgagtgaa aaccatgtcg cggataccca
    54721 gttgctcgag cgtctcaacc accacattac gcgcggtagt tttgcctgcg ccttccagcc
    54781 cctcaatgac gatatactta ctgcgcattt ttttccttaa gcactttcag ataatcctgc
    54841 acagacttgt tatgactggc aagattggta ttaaacgtgt gaccaccttt accatcggcc
    54901 acaaaataga gatacggcgt ttttgccgga tgcgcagcag ccttcagcga atccgccccc
    54961 ggcgtagcta tcgcacccgg cggcaaaccg gtaatggtat aggtgttata cgctgtcggc
    55021 gtttccaggt ctgcacgaga aagtttgcca ttataacgct ctcccatccc gtaaatcacg
    55081 gttgggtcgg tctgcaggcg cataccaatg cgtaaacggt tgataaatac cgaggcaacc
    55141 tgatcgcgtt cactggcaac ggcggtttct ttttcgataa ttgatgccat cgtcaccagc
    55201 tgatttttat ctttataagg cagaccgtcc gcacgccctt cccaggcgct atcgaccgct
    55261 ttcaccattt tcttgtgaac tcgcttgagt aacgcgacat cggtggtatt ggcggtatac
    55321 atcaaggtgt ctggccagaa ccaaccttca atccactccg ggttttccag ttcaagtgcc
    55381 tgcgctacgg tggcgtactt atcgtcactc agcgtgtgct tgatatacgg agcctcacgc
    55441 aattgcttga ggtagtcgct cagacgcatc ccttctacca gtcgcagcgg gaactgtgct
    55501 tctttaccgc tttccaacaa tttcagcatc tcgcgcacgg tcatctgcgg tgtaaagcgg
    55561 taagtcccgg ctttaaagtg agaaagatcc ggttcgatac gcagcagcca ttgaaaaacc
    55621 cgtgggcgat tgatgatctt atcagcataa agctgttcac cgagcgccag acgtccggtc
    55681 cctggcttca gggtaaatat cgtctcttct ttgataagca atttgctgtc ggcaagatgg
    55741 cgaaccttcc agacgcccac accagcggcg atacccagta ccaccagcaa taacaagata
    55801 atcaataaca cttttttcat gactaattcg ggcgctcaca aagtggggct aaatattcat
    55861 ataacgttgc tgacgaaaag gtgacatcgc cacaggcacg tacgggcatc actggcatta
    55921 acgcattaca aataaccatc tcatctgcct gcaacgcctc ctccagcgag gcttgcactt
    55981 cgacaagctg ataagaggat tgtgccagta aacggataca gaattgtcgc ataatgccgt
    56041 taacacctgc ctgatccagt cgcggcgtat aaactacatt gcccttccgc cagaacaaat
    56101 tagccgcaca gcattccgta acccaccctt cgctgtcaag gaccagcgcc tcatcagcgt
    56161 ttgtctgctc aagatgagag cgaatcaata cttgctcaag ccgattaaga tgtttaatac
    56221 ctgcaagatg aggattgcgc cccagccgca ccgggcttag cgccaacgtc atcccttcgt
    56281 tacgcaaacg gtcgtaatgt gcaggataag ccgtaacgga gagaatccgc gttgctggtc
    56341 cgctgttcaa tgtgctgtac cctcgcccgc cactaccgcg actgatcacg actttcagca
    56401 caccattttg ctgttctgct gccagcgttt tcatctcttg ttcaagctga ggccagaagt
    56461 cacaggaaat catcaaccgc tgacaagcat cctgtagtcg caggatatgc gccgataaca
    56521 aactgacttt accgtcgata actctggcgg tggtaaaaca accatcacca aactgcgttg
    56581 cccgatcgct tactgccagc gattcctgct tataaccgtt aattaagaac ataccggctc
    56641 cttatcagag aattgttagt gtggcagcat gttcactacg gaacaagtcg gaataaaagc
    56701 taagaaaaaa ggcccgcaag cggacctttt atatgggtgg aaaatgacaa cttagatctt
    56761 tttaaagatc aaagaaccat tagtgccacc gaagccgaag gagttacaca gcgtgtattc
    56821 cattccgctg acctgacgtg cttcatgcgg tacgaaatcc agatcgcaac cttcatccgg
    56881 gttatccagg ttgatggttg gcggaacagc ctgatcgcgc agcgccagga tggagtagat
    56941 agattctact gcacccgccg cacctaacag gtgaccggtc atagatttcg tggagcttac
    57001 caacacacgg cttgcagctt caccgaagat ggttttcacc gcctgcgctt cagctttatc
    57061 gccagccggc gtagaagtac cgtgcgcgtt aacgtagcca atctgactcg cttcaatgcc
    57121 tgcatcacgc agagcatttg ccattgccag agctgcgcct gcgccatttt ctggcggtga
    57181 cgtcatatga taagcatcgc tgctcatacc aaagccgacg agttcagcgt aaattttcgc
    57241 accgcgtttt ttcgcgtgtt cgtactcttc aagtaccagc ataccggcac catcgcccag
    57301 tacgaagcca tcacgctctt tatcccacgg gcggctcgcc gcttgcgggt tatcattgcg
    57361 ggtagataat gcacgtgccg cgccaaaacc accaacgccc agcggcgtac tggctttctc
    57421 tgcgccacct gcaaccatca cgtcagcatc gccatacgcg ataatacgcg cagcatggcc
    57481 aatgttgtgc acgccggaag tacaggcggt cgcgatagag atgcttgggc cacgcaggcc
    57541 atacatgata gtcagatgac ctgccaccat gttcacaatc gttgacggaa cgaagaatgg
    57601 gctgatctta cgtggaccac cgttcatcag agatgtgtgg ttttcttcga tcagtccgag
    57661 gccgccaatc ccggagccaa ttgcggcacc aatgcgggtt gcgttctctt ccgttatttc
    57721 aaggccagaa tcctgcatgg cctgaacgcc agcgacaatt ccatattgaa tgaaggcatc
    57781 catcttgcgc tgttctttgc gcgagataat gtcctcacag ttaaaatcct ttactaagcc
    57841 agcaaatttc gttgcatagg cgctagtatc gaaatggtcg attaggctga tgccactctg
    57901 accggcaagc agagctttcc aggtagactc tacggtattg ccgacaggag acaacatgcc
    57961 cagtccggtc acaactacac gacgcttaga cacgtttgtc ctccaaggag ggaaaaatga
    58021 ttctagtggg acaaaaagat aaaactcagg cggtcgaacg accgcctgga gatgttcact
    58081 tacgcctggt ggccgttgat gtaatcaatg gcagcctgaa cggtggtgat tttctcagct
    58141 tcttcgtccg gaatctcagt atcaaactct tcttccagag ccattaccag ctcaacggtg
    58201 tcaagagaat ccgcgcccag gtcttcaacg aaagaagcat tgttggtaac ttcttcctgc
    58261 ttaacgccca gctgttcgcc gataattttc ttaacgcgtt cttcgatagt gctcatactc
    58321 ttaaatttcc tatcaaaact cgctttcgcg atggttttcg tagtgtataa aatgttgaaa
    58381 aatttgcaac taaatcccgg caggtcttac cacgatttta cgttattttg aggccttacc
    58441 ccccaataac gcaaatcatt ttgcactaat tattactcat aaccacgcag actgcgcgca
    58501 acatgagcat tttgtgcaaa tcgcggtcag accatgtaca tcccgccgtt cacatgcaaa
    58561 gtttcacccg tgatgtaagc tgcttcgtcg gatgccagga atgcaaccgc gttggcgatt
    58621 tcctgtgcgc cgccgaggcg acccgcagga acctgcgcca ggatacccgc acgctggtca
    58681 tcgctcagcg cacgtgtcat gtccgtttca ataaagcccg gagcaacaac gtttacagta
    58741 ataccgcgtg acgcaacttc gcgcgccagt gatttactga agccgatcaa gcccgctttc
    58801 gccgcagcgt agttggcctg accgccattt cccatggtac caaccacaga accgatagtg
    58861 ataatacgac catgacgctt tttcatcata gcgcgcatta ccgcttttga cagacggaaa
    58921 acagatgaaa ggttagtttc gataatatcg ttccactctt catctttcat tcgcattaac
    58981 aggttatcac gagtgatacc ggcattattg accaggatat ccacttcacc aaattctgcg
    59041 cgaatttttt ccagaacaga ttcgatagat gccgggtcgg tcacattcaa catcagacct
    59101 ttgccgttgg cacctaaata atcactgatc gcctgagcgc cattttcact ggtcgcagtg
    59161 ccaataactt tcgcgccacg ggctgcgagc gtttcagcaa ttgcgcggcc aattccgcgg
    59221 cttgcaccgg ttaccagtgc gatttttcct tcaaaattca tgattttcct cttttaaagc
    59281 tcgagcgccg ctgccatcgc tgaaggttcg ttcagcgccg aggcggtcag ggtgtcgaca
    59341 atgcgtttcg tcaggccagt aagcaccttg cccgggccga cttcatagag atgttctacg
    59401 ccttgcgctg ccatgtactc aacagacttc gtccactgaa ccgggttata caactgacgt
    59461 accagtgcgt cacggatggc atcaccattg gtttcgcatt tcacatcaac gttattcaca
    59521 acagaaactg ttggtgcgtt aaaggtgatt ttcgctaatt ctactgccag tttgtcggct
    59581 gctggtttca tcagcgcaca gtgagacggt acgctcactg gtaacggcag cgcgcgtttt
    59641 gcgccagcag ctttacaggc agcgccagca cgctcaaccg cttctttatg accggcaata
    59701 accacctgtc ccggagagtt aaagtttacc ggagaaacga cctgaccttc tgcagcttct
    59761 tcacacgctt tcgcaatagg cgcatcatcc agaccgatga ttgccgccat agcgcccgtg
    59821 ccttccggta cggcttcttg catgaactta ccgcgcatct caaccagacg caccgcatca
    59881 gcgaaatcaa tcacgccagc gcaaaccagc gcggagtatt cccccaggct gtgaccggcc
    59941 atcattgccg gtgctttacc gccctgctgc tgccatacgc gatacagcgc aacagatgca
    60001 gtcaacagcg ctggctgggt ttgccaggtt ttattcagtt cttcagctga cccctgctgg
    60061 gtcagcgccc acaggtcgta gcccagcgcc gcagaagctt cagcaaacgt ttcttcgaca
    60121 attggatagc tcgccgccat atcagccagc attccaacgg tttgagaacc ctgtccaggg
    60181 aacacaaatg caaattgcgt catgttttaa tccttatcct agaaacgaac cagcgcagag
    60241 ccccaagtga atccaccgcc aaaggcttca agcagaacca actgccccgg cttaatgcgc
    60301 ccgtcgcgta cagcttcatc cagcgcgcac gggacagagg ccgcagaggt attaccgtgg
    60361 cgatccagcg tcaccacgac attgtccatc gacataccga gttttttcgc cgttgcactg
    60421 ataatacgca ggttagcctg atgcggaacc agccagtcca gttgagaacg gtcaagatta
    60481 ttcgccgcca gcgtctcatc aacgatgtgc gccagttccg ttaccgcaac cttgaagact
    60541 tcgttgcccg ccatcgtcag atgaattgaa ttctctggat tcacgcggtc ggcgtttggc
    60601 agcgtcagca attcaccata actaccgtcg gcatgcagat gggtggaaat gattcccggc
    60661 tcttcagagg cagccagcac cgcagcgccc gcgccatcgc caaaaataat aatagtccca
    60721 cgatctgttg gatcgcaggt gcgcgccagt acatcggaac cgacgaccag agcatacttc
    60781 accgccccag atttcacgta ttgatcggct acgcttaatg cataggtgaa acctgcacag
    60841 gctgctgcaa cgtcaaatgc cgggcaacct ttaatgtcca acatgctttg aatctgacaa
    60901 gctgcgctcg ggaaagcgtg cgtagcagaa gtcgttgcca caacgatcag gccaatctgg
    60961 tctttctcaa tgcccgccat ctcaattgcg cgtgtcgccg cttcaaagcc catggttgaa
    61021 acggtttcgt ttggcgcggc aatgtggcgt tcgcggatac cggtacgagt gacaatccac
    61081 tcgtcagagg tgtccaccat tttttccaaa tcggcgtttg tccgcacttg ttcgggcaga
    61141 tagctgccag taccaataat cttcgtatac atgtacgctc agtcactttt cggttatata
    61201 ccgtcacttg caaactgcga gttcgctggc agcgtcctgc taccgcagag ttccgctttt
    61261 gccaccgtcc agcagctcaa aaccagctgg gtatacagat tccaggcgag cggcaattcg
    61321 ctgaggaact tgtcgctgca ccgcctgcac tgcctgttca atcgcgaccg caaaagctcg
    61381 ctgattggct gcaccatgac tttttatcac cgtgccgcgc aatcctaaca gacaggcgcc
    61441 gttatactgg tcggggttga ggtgactgaa tcgcctcgtc aggctctttt gtagccaacg
    61501 ctttaataac agtagccacc acgaccgttt tttcccttca ccctgagatt tcagcagaga
    61561 aaggaacatc ctgacaacac cttccatcgt ctttaacgtg acatttcctg taaagccgtc
    61621 acaaaccagc acatctgtct tgccagttaa caactcattg gcttcaagat agccgatata
    61681 attgatagaa gggattgttt taagcaccgc tgaggcatcc cgaatactgt caagaccctt
    61741 tacttcttct tcaccaatat tgagcaacgc cacgcgagga ttgggaattt ccaccacctc
    61801 ttcagccaga actgagccca taatggcaaa ttgcaccaac attgtgctgt cacaatcgac
    61861 gttggcccct aaatcgagga ccaccgtttt gcccttttgc tgatgtggta ataccgtcac
    61921 cagcgccgga cgctcaatcc cctccagggg cttgagtaat aattttgcca gccccatcag
    61981 tgccccggta ttaccggcac tgacacaggc ttgcgctcga ccttctttca ccagctccag
    62041 ggccatacgc attgaactcc cacggctggc gcggatagct tgcgaaggcc gggcatcact
    62101 ggcgataact gactgcgcag gaataatctg cagacgcgaa cgttgttcaa agtcagcttt
    62161 agcaagtaat ggcgtgatgg cgtcgggatt gccgactaaa agaagagtga gttgcgaatt
    62221 agaattcagt gcctgcaatg ctgcaggcac tgtcacggaa gggccaaaat cccctcccat
    62281 gacatctaac gccagggtta gacgtgtcaa ggtatcgtcg ccgcccggtt tggtctttcc
    62341 ccagttgcct ggggaaaatc ctcactaagc ttcatcacgc agacgcgtga ttacttagcg
    62401 atgaccttgc ggccgcggta gtaaccgtcg gcagtgatgt ggtgacgcag gtgtttttca
    62461 ccagaagttt tgtctacaga caggctggtg actgcggtca gcgcgtcatg ggaacgacgc
    62521 atgccacgtt tggaacgggt tggtttattc tgttgtacgg ccatggacct tactcctcaa
    62581 ttacggttta tccccgatcc aacggggagc accaattact tacgctttaa gctggctaat
    62641 acggcaaatg ggtttggctt ttgcacttct tcaggcagtt caccaaagac catgtccgct
    62701 tcggacactt cacagtgttc agaatcatgc accggaacta ccggcaaggc gaggatgatt
    62761 tcatcttcaa ccattgcaag cagatcgatt tcaccgaatt cgttaacctc aatcggttca
    62821 tacgcttccg gcagtgcttc agcctgttcg tctgaacgca caggactaaa acaatacgtt
    62881 gtgtagacct gatgagtaaa cggcttcccg caacgctgac actcgagcgt taccgtcacc
    62941 ttcgcatcgc cgtttaacac tgcgagacgt tggttatcga tagcgaacga catggagcat
    63001 tccacatcac tgtccacact gactacggat tcggcgacgc gctcaacctg atcaggggta
    63061 tagataccct ggtaatcaag gcgtttttga gccgtacgaa ccggatcgag agtcaggggt
    63121 aattttacct tttgcatagg gcgcgcatat taactttgta acgtcataga gtcaaagaaa
    63181 aaggcagcct gtggttgcct tttgccaata attcgcacac attgcgggtt actgctttat
    63241 tttcgttcag tgctgataac gaacatcaac ctgcaaaacg aagcgcatag tttaaaatgg
    63301 ctatcatcaa tacgctatat ctgatggaaa aaaatatgcc taaacttatt ttagcctcca
    63361 cctcgccatg gcgtcgcgct cttctggaaa aactgcaaat ctcttttgaa tgtgcagcac
    63421 ctgaggtcga cgaaacccca cgcagcgacg aatcaccgcg acagttggtg cttcgactgg
    63481 cacaagaaaa atcgcaatct ctggcgtcac gttatccgga tcatttaatt attggttcag
    63541 accaggtatg tgttcttgat ggcgaaatca ccggtaaacc gttaacggaa gaaaatgccc
    63601 gtctgcaatt acgcaaagcc agcggcaata tcgtcacctt ctatactgga ctggcgttgt
    63661 ttaattcggc gaatgggcat ctacaaacag aagtggagcc ttttgacgtc catttccgtc
    63721 acctaagcga ggcggagatt gataattacg tgcgtaagga gcatcccctg cactgcgcgg
    63781 gtagctttaa gagtgaagga tttggcatta cgctgtttga gcgtttagag ggacgtgacc
    63841 ctaacacgct ggttggttta ccgctaatcg cgctgtgtca gatgttacgt cgggaaggga
    63901 aaaacccgct gatgggatag ttgtttgcgc cggatgcggt tacgtcgcat ccggcaatcg
    63961 gtgcaggcgg cctgatgcgg cgtaaacgcc ttatcaggcc aaccgcccat tatcaggctt
    64021 atatgcttgt attagcgcgc gttacgcagc ttttgcaaac aacgcttcaa accttcatcc
    64081 atcggcgctt cgatacgcat cacctcaccg gtccccggat gggtaaactt caacgctgcg
    64141 gcgtgcagga acagacgatt taatcccgtg cctgcttcag tgagctgtct gtcaaattca
    64201 cggtcaccgt agcgatcgtc aaaggcaatc ggatgacccg catactgtgt atgcacacgg
    64261 atctgatgag tacgtccggt taccgggcta caacgcacca gggtggcaaa tgcatagcgt
    64321 tcttccactt taaagcgtgt ttccgacggt ttgccttcct gactgacacg cacgatacgt
    64381 tcgccgcttt gcagaatatt tttcaataac ggcgcttgaa cgctcttcac atgcgactgc
    64441 cactgaccgc gcaccagcgc caggtaatct ttttgcattc ctttttcacg taattgctca
    64501 tgcagagaac gcaacgccga gcgttttttc gctaccagca acacacctga agtgtcccgg
    64561 tcgagacgat gaaccagttc aaggaaccgc gcttccgggc gcaacgcccg caaaccttcg
    64621 ataacgccga agcttaaacc actgccgcca tgtaccgccg taccggaagg tttattcagc
    64681 accaggatat gatcatcttc atataagatg acgtccgcca gcgccgccac cttttgcaga
    64741 tgtggcgaaa ccgcctcttc ttcccgctca gcaacgcgaa ccggtgggat acgcacctca
    64801 tcacccgctt cgagtttata ttcaggctta atacgttttt tgttcacccg cacttcacct
    64861 ttacgcaaaa tacggtaaat catacttttt ggtacgcctt tcaattgggt acgcaaaaag
    64921 ttatcgatac gttgccccgc ttcgtcagcg gtgatagcaa caatttttac ggatggagtc
    64981 tctgttttca tggtgggcga ttctaattag ccaacaggat tcgcgccact catttttcta
    65041 tgcttatatt tagttttgca ccttattact tcactacgtg atcactttat tgatggttat
    65101 taaaccaatc accagcaaga agtgaaaaaa ctgtgagtaa gcgggtgata aatggtaaaa
    65161 gtcatcttgc tataacaagg cttgcagtgg aataatgagg ccgtttccgt gtccatcctt
    65221 gttaaaacaa gaaattttac ggaataaccc attttgcccg accgatcatc cacgcagcaa
    65281 tggcgtaaga cgtattgatc tttcaggcag ttagcgggct gcgggttgca gtccttaccg
    65341 gtagatggaa atatttctgg agagtaatac ccagtctgtt tctttgataa ttgcgctgtt
    65401 tttccgcatg aaaaacgggc aaccgacact ctgcgcctct ttgagctgac gataaccgtg
    65461 aggttggcga cgcgactaga cacgaggcca tcggttcaca cccggaaagg cgttactttg
    65521 cccgcagctt agtcgtcaat gtaagaataa tgagtaagtt acgatgaaaa gaatgttaat
    65581 caacgcaact cagcaggaag agttgcgcgt tgcccttgta gatgggcagc gtctgtatga
    65641 cctggatatc gaaagtccag ggcacgagca gaaaaaggca aacatctaca aaggtaaaat
    65701 cacccgcatt gaaccgagtc tggaagctgc ttttgttgat tacggcgctg aacgtcacgg
    65761 tttcctccca ctaaaagaaa ttgcccgcga atatttccct gctaactaca gtgctcatgg
    65821 tcgtcccaac attaaagatg tgttgcgtga aggtcaggaa gtcattgttc agatcgataa
    65881 agaagagcgc ggcaacaaag gcgcggcatt aaccaccttt atcagtctgg cgggtagcta
    65941 tctggttctg atgccaaata acccgcgcgc aggtggcatt tctcgccgta tcgaaggcga
    66001 tgaccgtacc gaattaaaag aagcactggc aagccttgaa ctgccggaag gcatggggct
    66061 tatcgtgcgc accgctggcg taggcaaatc tgctgaggcg ctgcaatggg atttaagctt
    66121 ccgcctgaaa cactgggaag ccatcaaaaa agccgctgaa agccgcccgg ctccgttcct
    66181 gattcatcag gagagcaacg taatcgttcg cgccttccgc gattatttac gtcaggatat
    66241 cggcgaaatc cttatcgata acccgaaagt cctcgaactg gcacgtcagc atatcgctgc
    66301 attaggtcgc ccggatttca gcagcaaaat caaactgtac accggcgaga tcccgctgtt
    66361 cagccactac cagatcgagt cgcagatcga gtccgccttc cagcgtgaag tccgtctgcc
    66421 atccggtggt tccattgtta tcgacagcac cgaagcgtta acagccatcg acatcaactc
    66481 cgcacgcgcg acccgcggcg gcgatatcga agaaactgcg tttaacacca acctcgaagc
    66541 cgccgatgag attgctcgtc agctgcgcct gcgtgacctc ggcggcctga ttgttatcga
    66601 cttcatcgac atgacgccag tacgccacca gcgtgcggta gaaaaccgtc tgcgtgaagc
    66661 ggtgcgtcag gaccgtgcgc gtattcaaat cagccatatt tctcgctttg gcctgctgga
    66721 aatgtcccgc cagcgcctga gcccatcact gggtgaatcc agccatcacg tctgcccgcg
    66781 ctgctccggt accggtaccg tgcgtgacaa cgaatcgctg tcgctctcta ttctgcgttt
    66841 gatcgaagaa gaagcgctga aagagaacac ccaggaagtt cacgccattg ttcctgtgcc
    66901 aatcgcttct tatctgctga atgaaaaacg ttctgcggtg aatgcgattg aaacgcgtca
    66961 ggacggtgtt cgctgcgtga ttgtgccaaa cgatcagatg gaaaccccgc actaccacgt
    67021 gttgcgcgtg cgcaaagggg aagaaacgcc gaccttaagc tacatgctgc cgaagctgca
    67081 tgaagaagcg atggcgctgc cgtctgaaga agagttcgct gaacgtaagc gtcctgaaca
    67141 acctgcactg gcaacctttg ccatgccgga tgtaccgcca gcgccaaccc cagctgaacc
    67201 tgccgcgact gtcgtagccc cagcaccgaa agctgcaacg gcaacaccag cagctcctgc
    67261 acaacctggg ctgttaagcc gcttcttcgg cgcactgaaa gcgctgttca gcggtggtga
    67321 agaagccaaa ccgaccgagc aaccaacacc gaaagcagaa gcgaaaccgg aacgtcaaca
    67381 ggatcgtcgc aaacctcgtc agagcaaccg ccgtgaccgt aatgagcgcc gcgacacccg
    67441 tagtgaacgt actgaaggca gcgataatcg cgaagaaaac cgtcgtaatc gtcgccaggc
    67501 acagcagcaa actgccgaga cgcgtgagag ccgtcagcag gctgaggtaa cggaaaaagc
    67561 acgtaccacc gacgagcagc aagcgccgcg tcgtgaacgt agccgccgcc gtaacgatga
    67621 taaacgtcag gcgcaacaag aagcgaaggc gctgaatgtt gaagagcaat ctgttcagga
    67681 aaccgaacag gaagaacgtg tacgtccggt tcagccgcgt cgtaaacagc gtcagctcaa
    67741 tcagaaagtg cgttacgagc aaagcgtagc cgaagaagcg gtagtcgcac cggtggttga
    67801 agaaactgcc gctgccgaac caattgttca ggaagctcca gctccacgca cagaactggt
    67861 gaaagtcccg ctgccagtcg tagcgcaaac tgcaccagaa cagcaagaag agaacaatgc
    67921 tgataaccgt gacaacggtg gcatgccgcg tcgttctcgc cgctcgcctc gtcacctgcg
    67981 cgtgagtggt cagcgtcgtc gtcgctatcg tgacgagcgt tatccaaccc agtcgccaat
    68041 gccgttgacc gtagcgtgcg cgtctccgga actggcctct ggcaaagtct ggatccgcta
    68101 tccaattgta cgtccgcaag atgtacaagt tgaagagcag cgcgaacagg aagaagtaca
    68161 agtgcagccg atggtgactg aggtccctgt cgccgccgct gtcgaacctg ttgttagcgc
    68221 gccagttgtt gaagaaatgg ctgaagtcgt agaagccccc gtaccggttg ccgaaccgca
    68281 accggaagtg gttgaaacga cgcatcctga agtgattgcc gccgcggtaa ctgaacagcc
    68341 gcaggtgatt accgagtctg atgttgccgt agcccaggaa gtcgcagaac acgcagaacc
    68401 gatggttgaa ccgcaggaag agacggcaga tgcagatatt gaagaagttg ccgaaactgc
    68461 tgaggttgtg gttgctgaac ctgaagttgt tgctcaacct gccgcgccag tcgtcgctga
    68521 agtcgcagca gaagttgaaa cggtggccgc ggttgaacca gagatcaccg ttgagcataa
    68581 ccacgctacc gcgccaatga cgcgcgctcc ggcaccggaa tatgttccgg aggcaccgcg
    68641 tcacagtgac tggcagcgcc ctacttttgc cttcgaaggt aaaggtgccg caggtggtca
    68701 tacggcaacg catcatgcct ctgccgctcc tgcgcgtccg caacctgttg agtaataatt
    68761 agctcaaagt aatcaagccc tggtaactgc cagggctttt ttatttcatg tttgaatcaa
    68821 catattaaaa ttcatcaaat ggcatccttg ctaaccaaca atgcaaaata ggcaaaaaaa
    68881 atgcccgccc cggcgatccc agagcgggca gacaaaacat acccagtttc atgatatgtt
    68941 ttaaagcgag cttatttgtt gagctggaag agcgacaatc cctgcatatc ggtaaatgct
    69001 ttatacgatg cctgcaatgc cgtttgctgc atgatgtaag atgaaatagt tgcattccag
    69061 tccacatcaa ccagatcgct catctgctgc gtttgcccta aagcgcgatc gctacctaag
    69121 gaatccagcg actccagttt gttcagctgc gtgcctaatt ccgcgcgaac ggtaaggaca
    69181 ttattcagcg agtttttcag tccacggttg gttttatcca atgccgcagc ggcgatttct
    69241 ttatccgctt cgctatccgc gaccggcgtt ttcagggctg cgatggcgct atccagcatg
    69301 gcaaaaagat tggtttcaga agcgctaccg tctggttccg ctaccgcgtt actggtaata
    69361 ctgtcgaaaa ttttgtcgcc cgtatgtcct atcaccatcg aacgcgaagc atcgacctgt
    69421 tgcttaatac tttctgttcc gccaacataa tcaccgttag cctcactaaa cggcgcagtc
    69481 tctgttttat atgtagcgcc cattaccgtc agtggtgttc gccagattca gcaactggtc
    69541 acgaagcccc tgaatatccg tagccagcga ggcccggtca tcgtcactca aggtgccatt
    69601 gctggcgtag acaatttttt cctgagcatt ctggatagca gtggtgacct ggctaagtac
    69661 actctcttcc agtgacactt tttgagtggc gaaagtacgt gccagcgtgt actggctgtt
    69721 ttgcgcctgt gcctgggaga gaacaacagc ttgtgatgca gcaatgggat cgtctgaagg
    69781 gttaacgact cgcttacccg tcgacatctg ctcgccgtac ttcatccatt ctgcctgaga
    69841 attggtgatg ccgcgcatgt tttgctggta catcatctgg gtactgaaac gcattttatc
    69901 tccccttagc gaatgttaat cagcgcatca aaaatcgcgt ttgccgtctg cagaacctgc
    69961 gcattcgcca ggtaatactg ctgaaaacgt tgcagatttc cgtactcctc atcgagattg
    70021 acaccggaaa tcgactgctg ctgattggaa agctgcgtca ccacattacc ttgcgtggtg
    70081 ctactggttt tcaacgtcgc ggttttatta ccgatatcac tcactaacga ggcataagcg
    70141 tcgttaaagg atttcgcacc gcccaccgtt ttactgttgc tttgcagatc cagcagggcc
    70201 tgaccgttgc ggttgtcgct atcacccgca tcttcttcgc tcgccatcgc gattttcgct
    70261 tcgtcggtga ttaatacatc catgttgacg atggcgtcac ttactggttt cagcgtgaag
    70321 ctgtcgttaa cggcaggcgt tcctgtaaac gtcaactcca gaccatcaaa tgccacttta
    70381 ccgttggcat ctggtgtcgc cgtaaaagtg gtattgctgg caaggcgggt gacctgccac
    70441 tgattattat cgaacgagat tttgtaatct gtcgccagta ccacggaggc atcagttacc
    70501 gtggcaccga tcgcaacgtc acctttgttt ttcgtgtttt gcagaaccgc gggcttaccg
    70561 atagtaaaga aatcttcacc agcatcgccg ttagcatcaa atccggcttt gtgttggctg
    70621 ttgaaagcct cggcaaatgc cagcgccagt tgtccaagcg tattacgcgt ctggtccaga
    70681 tcctgagaac ggaatgtcag aatgccgccc agcgacccgg tattcagtaa tttctccggg
    70741 atctcaatat tgcctgccgt cccatcaacg taagcgacag tcgtacgaga agggtcggcg
    70801 ctggaaggaa ctgccgccag ttgccgcgcc gtacttccct gaaccagtga gtaaccattg
    70861 gcatcgtgat gttataagta ccgccatcct gaacgctgac ttctacgcca acaatctggt
    70921 ttaattcgct caccagttga tcgcgttgat ccagcagatt gttaggtgac gcccctgccc
    70981 ccacgcctgt caggcgcgag atttgatcgt tcaggctggc aatttgttta gcgtaattgt
    71041 tgatctgatc aacgctggca cctatcgcga tattgacctg tttgtcctgg tcgcgcagat
    71101 attgatcggt ggttttaaac tgattcacca acccttctga tttcccaatc agcgcctggc
    71161 gcgccgccgg gtcttccgcg ttactcacca gcgtttgcag gctggtgaag aaatcctgca
    71221 tctgtgttgc cagcgaagag gtactggtgg agagcatatt gtcgattttc gacatctgct
    71281 catagcgggc ggtcaggccg ctactttgcg tctgcgcagc acgtaactgg ttggtaataa
    71341 acgtatcata ctcacgctgc acaccagaaa cgtagacgcc attgccaacc cagccgccag
    71401 cgcccaacgt gctattggcc tgcgccataa tagtggtttg gcgggtatat ccggcaacgt
    71461 tatagctgga gatattatta cttgccgtat ttaacgccgc ctgggccgcg ttcagtccgc
    71521 tcatggcgtt attgatcaag ctggacatgg aggttcctta taagccttca attacagagt
    71581 attatcggca gcgacccgcc ggacttgagt tattcagaac agattatcaa tgttcatgct
    71641 gtaggttttg ctcaccttgt cgcttatcga tttcatctgc tggatcatgt tggtgagttt
    71701 acgggcatag tgaggatcgg tagcatagcc cgcgtcctgt aggacctgcg ccccctgttc
    71761 cgcactcgcg gcggtcgtca cggcggcgta gcgcgggtta cgtgttaaca gcccaacgta
    71821 atccgacaat gcttccagat acgagctgta gacccgaaac ttcgctttta ctttcttcgc
    71881 ttcgccgttt tcatattcag tcgtggtgat ttcagtgact tgccctttcc agttgccaga
    71941 ggctttgaca ccaaacaggt tatagcttgg ctcgccgttt tcgcggcgga tttgccgttg
    72001 cccccaacca gattcaagcg cagcctgagc gaggatcaaa tgatgtggca caccgctttg
    72061 ctggcttgcc agttgggcgg gcagcgagag ttgcgcgagg aatgctctac tgtcacccgg
    72121 cagcgaatca tcgtagttac gtggtacggc cttttgcacc agctgcgaaa gcgtctggtt
    72181 ttgataacgc accacggttt cgagcgggaa tttcatcggt gctgctggcg tggactcctc
    72241 tggcaatggt tgttctggcg tcatctgttt aaccatcatc tctgcaagcc ccagaccttt
    72301 gcccgccgtc atctgttggg caatttgctg gtcatacata ctggtataca gacgagtgtg
    72361 ctcgctgctg aacaggccat cttttggtaa agcgtcgcgc atgcttttca acatcatctg
    72421 cacgaacatc ccttccacct gacgggccac cggacggata tttgccgccg gatcttcgct
    72481 cgctttcgcc tttagttcgt tgagtgattg cgcatcccag gccgcacttg ccagtagttt
    72541 gctgtcgctg atcatcagat gatttccagt tttgcccgca gacatcccgc actttgcatt
    72601 gattgcaaaa tagacatcag atccatcggc gtagcgccca gcgcattgag cgcacgcacc
    72661 acgttattga ggctggcgct ggaacgtacg ctttgcagcg aaccgccgct ctggcgtaaa
    72721 tcgatctgcg tttgtggagt aacaacagtc tgtccaccac caaacggtgt atctggctgg
    72781 ctgacattgg cctgacgatt aactgttact gagagatttc cctgcgctac cgcgcagctg
    72841 tcgagggtca cttcgcgatt catcaccacc gaaccggtgc gcgagttaat cactacttta
    72901 gcgtcctgcg gggtgacatt aacctgcata ttctggatat cggcaaggaa acggacctgg
    72961 gaactgttgc cacttggtac gcgcacctga atagtccgcg catctaacgc ggtggcgctg
    73021 ccatatccac gcacgcggtt gatggtgtca gcgatttgct gcgccatgct gaaatcttcg
    73081 tcgttaagtt gcaaattaag ggtattcccg acgccaaact ggctgggcaa ttcacgttca
    73141 ataaccgcac cattggtgat ccgtccaccg ttcagttggt taacctgaac actgctaccg
    73201 cctgcggagg ctcctgcgcc gccaaccaga atattgccct gcgccagcgc atacacctga
    73261 ctgtcaacgc ccttaagcgg agtcatcaac aacgtacctc cacgcaagct tttggcatta
    73321 cccatggaag aaaccaccac atcgatggtt tgcccctgac gtccaaacgg aggaagtgac
    73381 actgtcacca ttaccgcagc gacgtttttt agctgcatat tggtgcccgt cggaacggta
    73441 attcccagct gtgagagcat gttattaagc gtttgtgtgg taaacggcgt ctgggttgtc
    73501 tggtcaccag tgccatccag ccccactacc aagccatagc caatcagtga gttttgcctt
    73561 accccctgaa cactggtgag atcgcgaata cgctcagcct gagccgccgt cgtaaccagt
    73621 agaagaatta atgcagagag aaatttaatc accacagcct cacttacatt ggcgacaggt
    73681 taaggaagaa acgctgcaac cagcccatat tttgcgcttc gttaatgtag ccattgccta
    73741 cgtattcaat acgcgcatcc gccacctgag tagacggtac ggtattgctg ccgctgatag
    73801 tgcgtggatt aaccacgccc gagaagcgaa taaattcggt accctgatta atggcaatct
    73861 gtttttcacc caccacatgc aggttgccgt tgaccagtac ctggtcaacc gtcaccgtca
    73921 acgtgccgct aaaggtattg ctggcattgg ccccgccctt tccgttgaac gtgttaccac
    73981 cggaggcttc gacatcggca cgagcgttac caaacagccc ctgcaaatag cgcggcacag
    74041 tatcaaagcc aaaattagtt ttaccgtcac ggctggcatt cgcagaggag cttttgctgg
    74101 cgctgacgtt ctcctgcaac acgatggtca gcgtatcgcc aatattgcgt ggtcgacgat
    74161 cttcaaacag cggttgatag ccatagttaa tcggctgagc agactggaaa atagaaccgt
    74221 tggcgacggg cgtcggaccg ggaaccggtt gtgcactggt cgccccctgc accagcggcg
    74281 tggagggtat ccaggcgcag ccggttagtg aaagcaccaa caagctggaa atggcataag
    74341 tatgcgcagc gtttttttgc atggctatca tcttcaaaaa tcagtaaacc ggtgaacctg
    74401 ccaccggtta tgccttagag ttgcgtcagt ttttgcagca tctgatcggt ggtggacacc
    74461 gctttactgt tgatttcgta agcgcgttgc acctgaatca tattgaccag ttcttccgcc
    74521 acgttgacgt tagacgtttc aacataccct tgatacagca gtcccgcgcc gttcaggccc
    74581 ggcgtgcttt cgttcggtgc accggaggat tgcgtttcgg tgtagaggtt ttcgccaatg
    74641 ctctccagtc cggtatcatt cataaaggtg gtgagattga gctgcccaac ctgaaccgga
    74701 gctgcctggc cttgttgggt tacgctgacc acgccatcac gaccgatggt gatacttaac
    74761 gcattcgccg gaatggtgat cgctggctgc acctgaaaac caccagccgt caccagctgc
    74821 ccgttctgat ccacctggaa agagccgtca cggatatagg ctgatgagcc atctggcaac
    74881 attacctgga aaaagccctg ccctttaatc gcgacatctt tgctgttatt ggtctgcgac
    74941 agattgccct ggctgtgtaa gcgttcagtg gcgaccgggc gtacccccgt gccgatttgt
    75001 aatccggagg gtaaggtggt ttgttcggaa gactgtgccc ccggctggcg aatggtttga
    75061 taaagcagat cttcaaacac cgcgcgctga cgcttaaaac cgttagtact gacgtttgcc
    75121 aggttgttgg caatgacgtc catattggtt tgctgggcgt caaggcccgt tttggcgatc
    75181 cataatgaac tgatcatata atgtcctgta gtggattgct gacaagcaga gcagaagcac
    75241 gcgacagtgt gaatttcccc tcaccctaac cctctcccca aaggggcgag ggaatagtcc
    75301 gcgcgctttt attgcaatgt catcaatcgt tcaattaact catcgacagc agttggttgg
    75361 cacggcctgc gttatcatcg acgctgctga tcaccttcat ctgcatttca aaacgccgcg
    75421 cgctggcaat catgtcgctc attgccgcaa cggcattgac gttactgcct tccagaaccc
    75481 ccgacatcac acgcaaggtt ggatctgcct gcagtaccgg ccctcgcgtg gcctgggttt
    75541 ctgcgcttaa acgaaaaatg ccgtcgtcac cgcgctgcac ttcgctgccc gtggctttca
    75601 ccagtttcag acgccctact ggcgcaactg tatttgccgg atcgcccgga ttgagcgccg
    75661 agattgtgcc atcggcagca atcgtgattt ccgccccttc cggcacagca attggccccg
    75721 cctcgcctat caccggatgc ccctgaattg tcagttgccc ggtgggatca acctgaatgc
    75781 tgccattacg cgtataccct tcgctgccgt cagcggtctg cacggccagc catcctgctg
    75841 caacgcaacg tccagcgggc gagaggtgta atccatcttg ccgggcgtca tgtctgcacc
    75901 cggtgttgac gccgttacca gcgtgcgcgt gggcagtgaa agcccttcca ccggcactgc
    75961 gcgcaaggcg tttaactgcg cgcgaaaacc gggcgtagag gcattggcca gattactggc
    76021 ggttaccgcc tgttgattca gcgtctggct ggctgctccc atcgcagtat aaattgcgtg
    76081 atccattgag ctatcccgtc agcgattagc gtaagttaac ccgcgtgttg aggatctggt
    76141 cctgggtttt gatggtctgg gcgttagact tatagttacg ctgggcaacg atcatattga
    76201 ccagttcttt actgagatcg acgttggacg cttccagcgc accgttggtc agagtgccaa
    76261 agtttcccgt cccggctgtc cccaacagcg ccacgccaga agattgcgtc gcagaccaga
    76321 cgttgtcgcc ttcggatgcc agaccttcgt tgttggcaaa gttcgccagt acaatctgcc
    76381 ccagcagttg ggtttgttcg ttggaattgt tgccgacaac cgtaccgtca tcattgattt
    76441 gataactcac cagatcgccc ggtttgtagc cattctgggt ggttgccaca atgttgttag
    76501 cgccggtatt ttgctgcatg gagttgagga agctcagact aaacgtggcg ggttctgcac
    76561 cgttaattgc gccggttgcg atattattcg ccatcgcacc atccactaat gtgccattag
    76621 cattaaattc cagtgttgtc gctgtcttcg caatgctgtt tggatcactg ctatcctggg
    76681 tgtagacctg ccagttatta tccccggtct tcacaaaata gacgctcatg tcatgagcat
    76741 taccctgact gtcgaaaaca gtcaccgaac cttttttgtt atagctatcc gcattgctgg
    76801 cgctgaatgg cgtaacagta ggaagcggat cactggaatt caggttgatc tgcatcgacg
    76861 ccgtggtggt agttttcgct gccatcaggg tattcgggat cgaaatattg gtcggattcg
    76921 ccccttgctg aatagtcggc ggcgtaccgg ttaccgggta acccgtcagc tgtaaacctt
    76981 gcgtattcaa caggttacgg ttttcatcca gcttaaattg tccgttacgg ctgtagaaca
    77041 ccgaaccgtt gctgtctacc agacggaaaa aaccgttctg gctgatagca acgtccagac
    77101 ctcgcccggt gttggtggtc gtgccatcgg taaagtcctg agtgataccg gcaactttta
    77161 cccccagtcc cactttcgaa ccggcaaaca tatcggcaaa agaggccgtg cctgatttaa
    77221 agccgtaggt ggcggagttg gcgatattgt tgccaataac atcgaggttg gtggcggcag
    77281 cgtttaatcc gctaaccgct tgagaaaagg ccatgactga ctcctgaaat gtgaaggctt
    77341 aaattatctg ccgtacttcg tcgagggtgg tggtgccgta agtgccgaga tccagcgtat
    77401 taccgctgtt gccgcggatc acaccctgca ccagagcaaa ctgcagcggc tgggcaacca
    77461 gttgtgtacc accgttactg gcgctaatcg ctacattgta agaaccgttc ggcgcagtgc
    77521 tgccatcagt caacgaaccg tcccaggtga aactgtgaac tccggcggtc agttcaccaa
    77581 tatcaatggt gcgcacaacc gcgccatttt tatcggtgat ggtggccgta actttgtctg
    77641 ccgcctgttg cagctcaaca ccaaacggcg tggtcgtggt cacagcccct tcttcactgc
    77701 cggttcccgc aagaacagtt gtgccgggga tcatcacgcc gtgaccgatc aggttactgg
    77761 cctgtaacga ctggctgtta tcaatctgtc cggaaataga tccgagcgtg gtattgagtt
    77821 tttcaatccc gctgaccgtg ctgatttgtg ccaattgcga cgtcagctcg ttgttttcca
    77881 ttggattggt cgggtcctgg tttttcagct gcgccaccag caaagtcaga aaactgcttt
    77941 gtaaatctgc ggcgttgctg cccgtgagcg aactactgct ggtggtactg acgccggtat
    78001 ttgtcggatc ggtggtggtt accgcaatgg acatagcttt ctcctttatt gaccgagcgt
    78061 aagggttttc agcatcatgc ttttcaccgt gttgagcact tcaacattgg cctgatagct
    78121 gcgtgacgcc gacatggtgt taaccatctc cccgacgaca tcaacgttcg gcatttttat
    78181 gtagcccttt gcatctgcca gcggattacc tggttcataa accagtttgt ccggggcctg
    78241 actttcttaa catcggcaac ctttacgccg cctgtcgcag cacctggtgc agcgttaacc
    78301 tggaatacca cctgttttgc ccgatatggc tgtccatcag gaccggtcac gctatcagca
    78361 tttgccagat tactggccgc cacgttcagg cgctgggact gggcagttaa cgccgacccg
    78421 gcgatatcaa aaatattcag cagtgccatc cgttaatttc cgctctgtaa aacgttcatc
    78481 atgcctttga tttgcccgct caacgcgcta aggctcatct ggtattgcag gctgttatcg
    78541 gcaaactggg tgcgttcgcg atccatatcg acggtattac cgtcaagcga aggctggtcc
    78601 ggaatacggt attgcagttc tgcggtagga ggcgtcagcg cctgcgccgg gatgtgctgc
    78661 gttgaggtca tcgtcagtgc aaccacactg gttgcatccc gtccacgttg catgactttt
    78721 ttaagttcac tggcaaaatc gatatcgcgc gcctgataac caggggtatc ggcattggcg
    78781 atgtttgctg ccagcacttc ctgacgctgg gcgcgcagat tgagcgcctc ttgttgaaaa
    78841 cgtaaggcgg cgtcgagctt atcgagcata tctcctccgc aggtatcaaa attctgccat
    78901 cagcttaaat gcctttacgc gcgcgttatc ggcggaataa acgcaaaatg ggtcgctatt
    78961 tatgccgttg atggtcattg cggacaggta caattcacgt tgtagaaatg gctgggggcg
    79021 aaaatgctga caataaaacg tagcgtggcg atcatcgcga tactgttcag tccgttaagt
    79081 acggcgagca atctcacatc gcaattgcac aactttttta gcgcccaact cgcgggggta
    79141 agtgatgagg ttcgtgtttc tattcgtaca gcgcccaatc tactaccgcc atgcgagcag
    79201 ccattgcttt cgatgagcaa taattcccgc ctgtggggca atgtgaatgt gttggcacgc
    79261 tgcggtaacg acaaacgata tttacaggtt aatgtgcagg ccacaggaaa ttatgtggtt
    79321 gccgcgatgc ctattgtgcg gggaggaaag ctggaagctg gcaatgtcaa actgaaacgt
    79381 ggacgcctgg ataccctgcc accgcgtacg gtgctggata tcaatcaact tgttgatgcc
    79441 gttagcctgc gcgatctatc acccgatcaa cctatccagt taacccagtt tcgccaggca
    79501 tggcgggtaa aagcgggaca acgcgtcaat gtgatcgcca gcggtgatgg gtttagcgcc
    79561 aacgcagaag gtcaggcgct gaacaatgca gccgtcgcac agaatgcgcg ggtgcgcatg
    79621 gtatcggaac aggtagtcag cggcgttgtt gatgcagatg ggaatattct tataaacctg
    79681 taagctgtta aagattaccc gtcccttgcc gataaataag caacacatga taaaagcgcc
    79741 ctcaatgagg aataaaccat gagtattgat cgcacttcgc ctctgaagcc tgtaagcacc
    79801 gttcaaccgc gcgaaaccac cgacgcgccg gtaacgaaca gccgggcggc aaaaacaacc
    79861 gcctccacca gcaccagtgt gacgttaagc gacgcgcaag caaaactgat gcaacccggc
    79921 agcagtgata tcaatcttga acgtgtcgaa gcgttaaaac tggcgattcg taacggtgaa
    79981 ctaaaaatgg acaccggcaa aattgccgat gcgctgatca acgaagcgca gcaagacttg
    80041 cagagtaact gatcgtatga cacgtcttgc agagatcctc gaccagatgt ccgctgtgct
    80101 taacgatctc aaaacggtaa tggatcaaga gcagcaacat ctctctatgg ggcagatcaa
    80161 cggcagccag ttgcaatgga ttacagaaca aaaaagctca ctgctggcga cgctggatta
    80221 cctcgaacag ttacgcaaga aagaacccaa tacagcaaat agcgttgata ttagtcaacg
    80281 ctggcaggaa attactgtga aaacgcagca actacgccaa atgaatcaac ataacggctg
    80341 gttactggaa ggacagattg agcgcaatca acaggcgctg gaaatgttga aaccgcatca
    80401 ggaaccgacg ctatatgggg cgaacggtca gacctcaaca ccccatcgcg gcagtaaaaa
    80461 gatttcgatc tgaatattat ctccggcctg cactgcaggc cggaatgcat tgttacaccg
    80521 tccggcgggc aaattcttta actttgaagc ccagtaccgc cagtgcagcg aagtacgcgg
    80581 caatccccgc cagcacgacc gccattaaac gcagtaagcg ccagggcatg gtacccaatg
    80641 accactccgg catgatatgt aacataccta aaagcacgcc agacatcacc agtaccgcca
    80701 ccaccagacg caacagaaac gccatccagc cgggttgcgg agtaaagatt ttctgcttac
    80761 gcaactgcca gtaaagcagc gaagcattca gacacgccgc cagaccaata gaaagtgaca
    80821 gcccggcatg tttcaacgga ccaataaacg ccaggttcat caattgcgtt aaaatcagcg
    80881 taacgatggc aattttcact ggcgttttaa tgtcctggcg ggaataaaag ccaggagcca
    80941 acactttcac tacaatcagg ccaatcaaac ccaccgagta ggcaattaac gcccgctggg
    81001 tcatcagcgc atcaaacgcg gtaaatttac cgtactggaa cagcgaaacg gttaacggac
    81061 ctgaaagaat gcccaacgca accgcactcg gcagtgccaa caggaaacaa agacgcaacc
    81121 cccagtccat caaacggttg tattcatcat gattgccact ggcaaaactt ttcgacagcg
    81181 acggcagcaa aatggtgcca agcgccacgc ccagcacacc ggagggaaac tccattaagc
    81241 ggtcggcgta atacatccaa gacaccgaac cggaagcaag aaacgaggca aaaatggtgt
    81301 tgatgattaa ggagatctgg ctcacagaga cgccaaggat cgccggtccc atctgtttca
    81361 ccacgcgcat tgctccagca tcgtggaagt taatgcgcgg caggaccagc atgccgatct
    81421 tcttcaggtg cggtagctga tacaccagct gcagaatgcc gccgaccgta acagcccacg
    81481 ccagcgccag caccggtggg ttaaagtacg gtgcggcaaa cagcgcgaaa ccaatcatgc
    81541 tgatgttaag cagtgttggc gcaaacgccg gaatcgagaa gcggttccac gtattcagaa
    81601 tcgctcccac cagcgacgcc agggagatca gcaagatata gggaaaggta atctttagta
    81661 gctggctggt cagggcaaat ttgtcagctg tgtcagcgaa gcctggcgcg gtcaccatga
    81721 tcacccacgg tgcggcgagc atgccagcga ccgtcacaac cgccagcgca agtgtcagca
    81781 ggccagaaac ataagagaca aagacccgcg tggcgtcttc accctgctta cttttatatt
    81841 ccgccagaat cggtacaaat gcctgggaaa atgccccttc ggcaaagata cggcgtaaca
    81901 agttaggaag tttaaaagcg acgaaaaagg cgtcggttgc catccctgcg ccaaagattc
    81961 tggcgacaat tgcgtctcgt gcgaagccaa gcacacgcga aaacatggtc atcgagctga
    82021 cggcggccag cgattttaat aaattcatcg gtgttctaat ccggacccac gggcaaaacg
    82081 cctgcaaggc aggcgctgaa agaggcgaat agtctaccta gattatggtg aattgctacc
    82141 gccagatgtt acagggttta ttcactcatc gcatcgcgcc agatcttgtc aacgatacgt
    82201 tgcgccagca ctgcctattc gccggcggtt tgcggaactg tctgatttta cacgcattca
    82261 atgaagtgcc gcgcacagcc gacaaatcca cgttgctcaa gagtgctctg ccaaccagga
    82321 atcggtttat tcactacacc ctgcccgcgc tcctcacgcc attcgcgcat atccgtaatg
    82381 tcgatgagcg caccgtcagt cacggcctgc acggtttcac gctgacttcc ggcacggcga
    82441 tgcatacaag tggtgatctg caaaggaccg gccgaaaaat ggtgctcggc aaacagcatt
    82501 tcgccagcgt cgttagtcag tagcgtaccg ccatccagag aggctttgcc gcccgacaac
    82561 cacagcgcgg tatccaccac atgcagataa tcatccagca acgtgaaata aagatcgtgt
    82621 ggcccgacgc tattgctacg atgtttatcc attcttagcg aggctgcggt ggcgagttgc
    82681 gtttttaact caccgtagag tggtgcgaaa cgacggttaa aaccgaccat caacgtcagt
    82741 tttttccgcg ccgccagttc caccagcctt tcagcatcgc gcagattttc tgccagcggt
    82801 ttatcgacac agacatgtac ccccgcattg agtaacgtac tgaccacgtc aaagtggctg
    82861 gcggtgctgg agtgcacaaa aaccgcatca caactggcgg caaggctgga taacgaatcg
    82921 gcataaggaa tgcgccagct gtcacaaatt ggcagggctt tggcgcgcgt aggcgaccag
    82981 gctccttgta acgtccagtc agacgctgcc gccagcaccg gtaaccacgc tttttgcaca
    83041 atgccgccta atcccactac gccgatacgt aattttttca ctttaatctc ccagatgggc
    83101 cagcaacgaa tcaagacgtt gtttcagttc tgccacttcg atttccaggg cttcgacgcg
    83161 ggtctgtaaa tcaccgtcaa ccatgttcga catagccgtt accgccggct gatcttcaac
    83221 ctcaccactg aaaagatgca tgtagcggct ttcgcgttta cccggttcgc gggccagacg
    83281 caccacaaaa ggaccatctt cgcgattcgc cagttgttcc agcgtcgact ccacttccgc
    83341 catatcgctg aattcataca ttcgcgcggc gcggctgcgc agttcacccg gcgtctgggc
    83401 accacgcaat aacaacgtgg tgattaacgc cacttctgcg gcgctcagtt tcagatcgcc
    83461 aaattctgaa ttacaaaaac gttgctcata tttggtgacc cgattaccaa aaccgctcac
    83521 tgtgcgtaga taatgacgtt tgaccagatt atccagctgt tcctgcactt cggattcact
    83581 cagattcatg accggttcac ggttcgtttt ctgattacag gccgtgacta caccattgac
    83641 tgagagcggg tattgctccg gcgtggtcac ctgcttttcc agcaagcaac caattacgcg
    83701 agcttccagg gcagtaagtt gatatttcat gagattctcc ttagcggccg ggcgtccagt
    83761 ctggggtagt taatgccgtc agtacgtgat cgcgccattg tccatcaatc aacagatagt
    83821 ctttcgcata gccttctttt tcaaaaccca gtcgcgccag taaatcaccg ctgcgtttat
    83881 tgtgcggcat ataattagcc ataatgcgat gaatatgttg ggtgcgctgc atataacgaa
    83941 tggctgcggt cagggcttca aacatgagtc ctttgccctg ccatttttgc ccaatcgaat
    84001 aaccgagata gcaggcatga aaagagccgc gaacaacatt ggaaaaattg gcaacgccaa
    84061 taatctcttt ttcgtccggg tcgaataagc caaagtagaa agctgaacct tgtttatgaa
    84121 attcgttaat catccctagc ctggcctgcc agcctgatgg ataacagtgg ctttcgtcgc
    84181 gcactggctc ccagggcttg aggaaatggc gattctctgc gtaataatcc gccagacgcc
    84241 aggcatcacg atcatgcacc agacgcacga ccagtcggtc tgtggttaag cgcacttttg
    84301 gcacgttact gcgatagcca aacattctat acctactcct tcccgtaacg tctttaatac
    84361 gctgtaagta ttactatacc tgcgcaacca gaccctgtga aaagtccaaa atgccatttt
    84421 ctgcattatt cacatatttg ataaaaactc tctatcaaag cgacgttttg ataaaaaaat
    84481 actgtccccg aagtgatggg aaaagtctta gcggcaatgc agaatagaaa gtgctcatct
    84541 tttatttccc gggaggggaa atgtcccgcg tatcgcaggc gaggaacctg ggtaaatatt
    84601 tcctgctcat cgataatatg ctggtcgtgc tggggttctt tgttgtcttc ccgctgatct
    84661 ctatccgctt cgttgatcaa atgggctggg ccgccgtcat ggtcggtatt gctctcggtc
    84721 tacgccagtt tattcagcaa ggtctgggta ttttcggcgg cgcaattgcc gaccgctttg
    84781 gtgccaaacc gatgattgtt accggtatgc tgatgcgcgc cgccggattc gccacaatgg
    84841 gtatcgccca cgaaccgtgg ctattgtggt tttcatgcct gctctcggga ctcggcggca
    84901 cgttgtttga tccgccgcgt tcggcgctgg tggtgaaact aatccgtcca caacaacgtg
    84961 gtcgtttttt ctcgctgttg atgatgcagg acagtgccag tgcggtcatt ggcgcattgt
    85021 tggggagctg gctgttgcaa tacgactttc gcctggtctg cgccacaggg gccgttttgt
    85081 ttgtgctatg tgcggcgttc aatgcgtggt tgttaccggc atggaaactc tccaccgtac
    85141 gcacgcccgt tcgcgaaggc atgacccgcg tgatgcgtga caagcgtttt gtcacctatg
    85201 tcctgacgct ggcgggttac tacatgctgg ctgtacaagt gatgctgatg ctgccaatta
    85261 tggtcaacga cgtggctggc gcgccctctg ccgttaaatg gatgtatgcc attgaagcgt
    85321 gtctgtcgtt aacgttgctc taccctatcg cccgctggag tgaaaagcat tttcgtctgg
    85381 aacaccggtt gatggctggg ctgttgataa tgtcattaag catgatgccg gtgggcatgg
    85441 tcagcggcct acaacaactt ttcaccctga tttgtctgtt ttatatcggg tcgatcattg
    85501 ccgagcctgc gcgtgaaacc ttaagtgctt cgctggcgga cgcaagagct cgcggcagct
    85561 atatggggtt tagccgtctg ggtctggcga ttggcggcgc tattggttat atcggtggtg
    85621 gctggctgtt tgacctgggc aaatcggcgc accagccaga gcttccgtgg atgatgctgg
    85681 gaattattgg catcttcact ttccttgcgc tgggttggca gtttagtcag aaacgcgccg
    85741 cgcgtcgttt gctggaacgc gacgcctgat ttgatactcc cccctgactc ctccatactg
    85801 aaagtgaact gacagccaaa tggaggagtc acgtgaagct atacatttac gatcactgcc
    85861 cttactgcct caaagcccgc atgattttcg gcctgaaaaa tatccccgtc gaattacatg
    85921 ttctgctcaa cgacgacgca gaaacaccca cccggatggt cggtcaaaaa caggttccca
    85981 ttctgcaaaa agatgacagc cgctatatgc cagaaagcat ggatatcgtt cactatgtcg
    86041 ataaactcga cggcaaaccg ttactgaccg gcaaacgttc ccctgccatt gaagagtggc
    86101 tgcgcaaggt caatggctac gtcaacaaac tgctgttgcc gcgttttgcc aaatcggcat
    86161 ttgatgagtt ttctactccc gccgcgcgca aatatttcgt cgacaagaaa gaggccagcg
    86221 cgggtaattt tgccgacctg ctggcccact ctgacggtct gattaagaat atcagcgatg
    86281 atttgcgtgc gctggacaaa ctgatcgtca aaccgaacgc cgtgaatggc gaactttcgg
    86341 aagatgatat tcagctattt ccgctactgc gtaatctgac gctggtagcc ggaatcaact
    86401 ggccaagccg cgttgctaat taccgcgata atatggcgaa acagacacaa atcaatttgt
    86461 tatcatcaat ggcgatttaa tcctgacccg ccgcggcagc ctgtattcgc gggttgccgc
    86521 cgttttcagt tttttctgct ggcgagttta tcgctggtga cacatgctgc gttgtgtcag
    86581 gatattgacg cagatttcat gtcgaggact accatgaaca agtttttatt tgccgctgca
    86641 ttgatcgtca gtggcctgct cgttggctgt aatcaactca cccaatacac catcaccgaa
    86701 caagaaatta accagtcgct tgcgaaacat aataatttct caaaagatat cggtttaccc
    86761 ggcgtggctg acgcccatat tgttctgaca aacttgacca gccaaattgg tcgcgaagag
    86821 ccgaataagg ttaccctaac cggagacgcc aatctggaca tgaactccct gttcggtagt
    86881 cagaaagcga ccatgaaact gaagctgaaa gcgctgcccg tgtttgataa agaaaaaggt
    86941 gcgatcttcc tgaaagagat ggaagtggtc gatgcgacgg tacaaccgga aaaaatgcaa
    87001 acggtgatgc aaacgttgct tccctatttg aaccaggcat tacgcaatta ctttaaccag
    87061 caacctgctt acgtcctgcg cgaagatggc agtcagggcg aagcaatggc gaaaaaactg
    87121 gcgaaaggca ttgaagtgaa gccaggcgaa attgtcattc cgtttacgga ttaatgacga
    87181 gggcgcattc gcgcccttta tttttcgtgc aaaggaaaac gtttccgctt atcctttgtg
    87241 tccggcaaaa acatcccttc agccggagca tagagattaa tgactgcacc atcccaggta
    87301 ttaaagatcc gccgcccaga cgactggcac cttcacctcc gcgatggcga catgttaaaa
    87361 actgtcgtgc cgtataccag cgaaatttat ggacgggcta tcgtaatgcc caatctggct
    87421 ccgcccgtga ccacagttga ggctgccgtg gcgtatcgcc aacgtattct tgacgccgta
    87481 cctgccgggc acgatttcac cccattgatg acctgttatt taacagattc gctggatcct
    87541 aatgagctgg agcgcggatt taatgaagcc gtgttcaccg ctgcaaaact ttacccggca
    87601 aacgcaacca ctaactccag ccacggcgtg acgtcaattg acgcaatcat gccggtactt
    87661 gagcgcatgg aaaaaatcgg tatgccgcta ctggtgcatg gtgaagtgac acatgcagat
    87721 atcgacattt ttgatcgtga agcgcgcttt atagaaagcg tgatggaacc tctgcgccag
    87781 cgcctgactg cgctgaaagt cgtttttgag cacatcacca ccaaagatgc tgccgactat
    87841 gtccgtgacg gaaatgaacg gctggctgcc accatcactc cgcagcatct gatgtttaac
    87901 cgcaaccata tgctggttgg tggcgtgcgt ccgcacctgt attgtttacc catcctcaaa
    87961 cgcaatattc accaacaggc attgcgtgaa ctggtcgcca gcggttttaa tcgagtattc
    88021 ctcggtacgg attctgcgcc acatgcacgt catcgcaaag agagcagttg cggctgcgcg
    88081 ggctgcttca acgccccaac cgcgctgggc agttacgcta ccgtctttga agagatgaat
    88141 gctctgcagc actttgaagc attctgttct gtaaacggcc cgcagttcta tggcttgccg
    88201 gtcaacgaca cattcatcga actggtacgt gaagagcaac aggttgctga aagcatcgca
    88261 ctgactgatg acaccctggt gccattcctc gccggggaaa cggtacgctg gtccgttaaa
    88321 caataaaaat cgtagcttcc tgttgtcatt aggttatttt acctgtataa ataaccagta
    88381 tattcaacag ggggctatta tgcgaattga agtcaccata gcgaaaactt ctccattgcc
    88441 agctggggct attgacgccc tggctggcga actttcccgc cgtattcagt atgcgtttcc
    88501 tgataatgaa ggccacgtat cggtacgtta tgccgcagcg aataatttat cggttattgg
    88561 cgcaacaaaa gaagataaac agcgcattag cgaaattctc caggaaacgt gggaaagcgc
    88621 cgatgactgg tttgtcagcg aataatatgc agtgattttt gtttttgccg gatcgccccg
    88681 gcttttttat tttacttacg aattttgagt acaaaatttc tacacttcct caaaataatt
    88741 gcacaatatg ctaaaaaaat atgaatgaca tgctgtttta ttgttcatat aaatcttaaa
    88801 agcagaaatt tatctgttcc cccctttaaa aattcggtga atacccttac ttattggtat
    88861 actgaagttg cttcagataa aaacgcattg aacctcgaat aacgttgtct agtaacacga
    88921 attagggggc catgatggaa aaaaataatg aagtcattca gactcatccg ctcgtagggt
    88981 gggacatcag caccgttgat agctatgatg cgctgatgtt gcgtttgcac taccagaccc
    89041 caaataagtc cgagcaggaa gggactgaag ttggtcagac gctctggtta accactgatg
    89101 ttgccagaca atttatttcg atattagaag caggaatcgc caaaattgaa tccggtgatt
    89161 tcccggtaaa cgagtatcgg cgtcattaac atgacatgac catccaccca aaaggcacct
    89221 caccggtgcc tttaccattt ctgggttgta tatctgcatt cagttaatta ctctgcttaa
    89281 accatgagag cagagagaaa aaaatgaaat acgatctcat cattattggc agcggttccg
    89341 taggcgctgc cgccgggtat tatgcaaccc gcgccggttt aaacgtgcta atgaccgacg
    89401 cccatatgcc accgcatcaa cacggcagcc accacggcga tacgcgatta attcgccatg
    89461 cttatggtga aggcgaaaag tatgtcccgt tggtcctccg cgcgcaaacg ctgtgggatg
    89521 aactttcccg ccacaacgaa gatgatccca tttttgtacg ctctggtgtc attaacctcg
    89581 gcccggctga ctccgcattt ctcgccaacg tcgcccacag cgccgaacag tggcaactca
    89641 acgttgaaaa gctcgatgcg caagggatta tggcccgctg gccagaaata cgcgtcccgg
    89701 acaactacat cggcttattt gagactgatt ccggtttttt gcgcagcgaa ctggcgatta
    89761 aaacctggat ccaactggcg aaggaagcgg gctgtgcgca actgttcaac tgcccggtca
    89821 ccgcaattcg tcatgacgat gatggcgtaa ctattgaaac ggttgacggt gagtatcagg
    89881 cgaaaaaagc gattgtctgc gcgggaacat gggtaaaaga cctgctcccg gagctgcctg
    89941 tccagcccgt acgtaaagta tttgcctggt atcaggccga tggccgctat agcgtgaaga
    90001 ataaattccc ggcgtttacc ggtgaactgc ccaatggcga tcaatattat ggttttccgg
    90061 cagaaaacga cgcgttgaag attggcaaac ataacggagg ccaggttatc cattcagcgg
    90121 atgaacgtgt tccgtttgcg gaagtggcca gtgatgggtc agaagccttc ccgttcttgc
    90181 gcaatgtatt gccgggtatc ggttgctgcc tgtacggcgc tgcctgcacc tatgataatt
    90241 cgcctgacga agattttatt atcgataccc tacccggcca cgataataca ctgctcatta
    90301 ccggcctgag tgggcacggt tttaaatttg cgtcagtttt aggggaaata gctgccgatt
    90361 ttgcgcaaga ccaaaaaagc gattttgatt tgacgccatt caggctttcc cgcttccaat
    90421 aatcatcatt acggcctcca gaatgaggcc gtttttacag gagtatttaa tgcgtcggct
    90481 gttgcactat ctcatcaata atattcgcga gcatctgatg ctttatcttt ttctctgggg
    90541 attgttggca attatggatc tgatctatgt gttttatttt tagttttccg cactgacata
    90601 aactgacaaa tatttaatct ccctttcatt tagcgtgaaa aattgaatga gattattcct
    90661 gcttctacag cactcaccag acatctctga tgctgaaccc actaaactta tgaagattct
    90721 ttcattatta attcaaaatt attgaaataa aaataaatat cattcaattt gttatttttg
    90781 tgttgcaatt cattctccga cacgccattt tctttctctc gcaaattcgt attggatgca
    90841 cttatgtcat tcacaaatac ccctgaacgc tatggtgtta tatctgcggc cttccattgg
    90901 ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc tgtggatggt cacgctcagt
    90961 tattacgatg gctggtatca caaagcaccc gaactgcata aaagcatcgg tattttgtta
    91021 atgatggggc tagttattcg cgttctgtgg cgtgtcatat ctccaccgcc gggtccgctg
    91081 ccgagttatt cgcccatgac tcgtcttgcc gcaaaagctg gacatctcgc attgtgccta
    91141 ttgctgttcg ccatcggcat cagcggctat ctgatctcaa ctgccgatgg taaaccgatc
    91201 agcgtttttg gctggtttga cgtccccgcg acccttgccg acgctggcgc acaggcagac
    91261 tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg tagtactgtc tgttatgcac
    91321 ggatttatgg ccctgaagca tcatttcatc gataaagacg acactctgaa gcgcatgctg
    91381 ggaaagtcgt catctgacta tggagtataa aaaatgaaaa aaagcctgct tggtttaacc
    91441 ttcgcgtccc tgatgttctc tgccggttca gcggttgccg ccgattacaa aattgacaaa
    91501 gaaggtcagc acgcctttgt taacttccgc atacagcatc ttggctatag ctggttatat
    91561 ggcaccttta aagatttcga cggtactttt acctttgatg aaaaaaatcc ggctgccgat
    91621 aaagtgaatg tgacaattaa caccaccagc gtcgatacta accacgccga acgcgataaa
    91681 catcttcgca gtgcggattt tctcaatacc acaaaatatc cacaggcaac attcacctcc
    91741 accagcgtga agaaagacgg tgacgaactg gatattaccg gcgatctgac gctgaatggc
    91801 gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc agggcgacga cccatggggc
    91861 ggtaaacgtg caggctttga ggccgaaggc aaaattaagc tcaaagactt caatatcaag
    91921 acagatttag gtccagcttc tcaggaagtg gatctgatta tttcagtgga aggcgtacag
    91981 cagaagtaag agtgaaatgt tgccggatgg cacgcatccg gcaatgatat ttattctgtt
    92041 ggatcaggaa tacccagtgt tgtattcaga cgtccacgag acttattaaa gatcttattg
    92101 ccattttcac gtcctgcccg acggcgtcgc tgttcttctg gcggtaacgc gctttcttcg
    92161 cagcaaatct cactacaaca acctttgtat ttttccgcgc atactggaca ctgaataaac
    92221 agcaggtggc agccatcatt tttacagttg gtatggctgt cgcacggcgc gccgcactgg
    92281 tggcaatgcg cgataatctc atccgatata cgttcgccca tccgctcgtc aaaaacaaaa
    92341 tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg ccttacgggc gtattcaata
    92401 attccgccct cgatatgcca gactttgttg aatccgttat gtttcatcca ggcactggcc
    92461 ttttcacaac gaatgccgcc ggtgcagtac atgacgattt ttttatcttt atgtgcctgc
    92521 atcatctcga ctgcttttgg cagctgctca cggaaggtat ctgccggaat ttcgagcgcg
    92581 ttttcaaagt gccccacttc atactcatag tggttacgca tgtcgataaa tagtgcatcg
    92641 ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat actcaccaac attgctggca
    92701 tcaaagtgag gatcgtcaat accgtcggca acaatgcgat cgcgtacctt catgcgcagt
    92761 acccagaagg atttcccgtc atcatccaac gcgatattca ggcgtaaacc ctctaaagcc
    92821 gggtcgaagg catagagctg cgcgcgaaat gtttcaacat tgctcgcagg tacgctgatt
    92881 tgcgcgttaa tgccctcatg cgccagatac actcgcccaa aaacattcag cgcggtaaac
    92941 agctgatata aagcgtcacg ggtcgcctta ggatcggcga tgtggaaata cttgtaaaac
    93001 gaaatggtgg ttcgcggttc gctctcagcc aacattttgg cttttaacgc gtcgttggaa
    93061 atgcggttgt gtaacactgg catggtgtac ggttcctgcg agatgggaaa gtaaaaatcc
    93121 gcggcatgat atagcaatta tcgataatta acatccacat attttacgct acatttgcgc
    93181 attaaaaatt atttgttatt tacaagcgcg gcaatttcgc ccagtcttca gccacaaatt
    93241 ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc ccgtataggt tgaaaaacag
    93301 gattgatatg acgaatctac ccaagttctc caccgcactg cttcatccgc gttattggtt
    93361 aacctggttg ggtattggcg tactttggtt ggtcgtgcaa ttgccctacc cgattatcta
    93421 ccgcctcggt tgtggattag gaaaactggc gttacgtttt atgaaacgac gcgcaaaaat
    93481 tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc gaacaagaac gccgtaaaat
    93541 ggtggtgaag aatttcgaat ccgttggcat gggcctgatg gaaaccggta tggcgtggtt
    93601 ctggccggac cgccgaatcg cccgctggac ggaagtgatc ggcatggaac acattcgtga
    93661 cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc cattttctga cactggagct
    93721 gggcgcgcgg cagtttggta tgcaggaacc ggggattggc gtttaccgcc cgaacgataa
    93781 tccactgatt gactggctac aaacctgggg ccgtttgcgc tcaaataaat cgatgctcga
    93841 ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa ggcgaagtgg tctggtacgc
    93901 accagatcat gattacggcc cgcgctcaag cgttttcgtc ccgttatttg ccgttgagca
    93961 ggctgcgacc acgaccggaa cctggatgct ggcacggatg tccggcgcat gtctggtgcc
    94021 cttcgttcca cgccgtaagc cagatggcaa agggtatcaa ttgattatgc tgccaccaga
    94081 gtgttctccg ccactggatg atgccgaaac taccgccgcg tggatgaaca aagtggtcga
    94141 aaaatgcatc atgatggcac cagagcagta tatgtggtta caccgtcgct ttaaaacacg
    94201 cccggaaggc gttccttcac gctattaaat ctcccatgcc ggatgcttca gaatggcatc
    94261 cggcattacc acagcaaatc cccctgattt agcgataaaa gctctctgga ttgcgccccc
    94321 tggaagtcgg gcgcataatt agtgtgttta tcttttcttc ttatgttcac cgcgcctggc
    94381 gcaccaacag cggattgcta tgtcaccctg tgaaaatgac acccctataa actggaaacg
    94441 aaacctgatc gtcgcctggc taggctgttt tcttaccggg gccgccttca gtctggtaat
    94501 gcccttctta cccctctacg ttgagcagct tggtgttacc ggccactccg ccctgaatat
    94561 gtggtccggt attgtcttca gtataatggg ttcctaaaca catcatttta ggtattgaat
    94621 atgagacggg caatcagcta catcagattt tcaagtgaac gacaactgaa aggggactct
    94681 gtacgccgac aatctaagct ggttactgac tggcttgata agaaccctga gttttacttg
    94741 gattcatcct tgagctttaa agatttgggg aaaagtgctt ttagtggtaa gcacctgaaa
    94801 ggtggtctag gtgacttttt aaccgctatc gagaaagggc ttgttaaagc tggtgatacg
    94861 ctactaatcg agagtttaga ccgtctgtca cgtcaggata ttgatattgc ttctgagcta
    94921 ttacgccgta tcttaagggc tggtgtagat gtagtcacac tgagcgatgg agaacattac
    94981 acacgagaaa gtttaaaaga cccgttagcc ctgattaaaa gcattctgat aatgcagcgg
    95041 gcgcatgagg aaagtttgag aaagtctgag cgtgtccaag ctgcatggaa ccgtaaaaaa
    95101 gaactcatct ccgaaggtat caaggtatct aggcgttgtc ctgcatggtt aaggctgaat
    95161 gatgaccgtc gaacattcac aatcattcct gataaagtgg aagttgttaa acgagcattt
    95221 gacctgagat tacagggttt aagcttttgg gctatcacca gaactttaaa cgatgaaggt
    95281 cacttgtcat tgaatcaata cacacccaaa caaaaggggt ggtctgatac tgctgtaaag
    95341 aaactgctca gaaatcgtgc tgtgattgga tgtttcacac ctgctggacg tgaagaagtt
    95401 cagggttact accccgcaat aatcagtgag tcactattct atcgagtaca gcaactgaac
    95461 acagggcaat atggtagggc ttctgtatca agtaatccgc tatcagtcaa cttgttcagg
    95521 ggtatcatca aat
//
LOCUS       DAETQL010000002        94501 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.002, whole genome shotgun sequence.
ACCESSION   DAETQL010000002 DAETQL010000000
VERSION     DAETQL010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 94501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 94501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..94501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.002"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="K0W65_000107"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="K0W65_000107"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBI3093547.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="K0W65_000108"
     CDS             complement(1672..2730)
                     /locus_tag="K0W65_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI3093548.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="K0W65_000109"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="K0W65_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="K0W65_000110"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="K0W65_000110"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBI3093549.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="K0W65_000111"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="K0W65_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBI3093550.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="K0W65_000112"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="K0W65_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBI3093551.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="K0W65_000113"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="K0W65_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HBI3093552.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="K0W65_000114"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="K0W65_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBI3093553.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="K0W65_000115"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="K0W65_000115"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HBI3093554.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="K0W65_000116"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="K0W65_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBI3093555.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="K0W65_000117"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="K0W65_000117"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBI3093556.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="K0W65_000118"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="K0W65_000118"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBI3093557.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="K0W65_000119"
     CDS             complement(17699..18322)
                     /locus_tag="K0W65_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBI3093558.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="K0W65_000120"
     CDS             18619..18894
                     /locus_tag="K0W65_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093559.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="K0W65_000121"
     CDS             complement(18971..19213)
                     /locus_tag="K0W65_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBI3093560.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="K0W65_000122"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="K0W65_000122"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBI3093561.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="K0W65_000123"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="K0W65_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBI3093562.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="K0W65_000124"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="K0W65_000124"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBI3093563.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="K0W65_000125"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="K0W65_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24093..24827
                     /gene="ypdB"
                     /locus_tag="K0W65_000126"
     CDS             24093..24827
                     /gene="ypdB"
                     /locus_tag="K0W65_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HBI3093564.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="K0W65_000127"
                     /pseudo
     CDS             24840..25697
                     /locus_tag="K0W65_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="K0W65_000128"
     CDS             complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="K0W65_000128"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBI3093565.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="K0W65_000129"
     CDS             complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="K0W65_000129"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBI3093566.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="K0W65_000130"
                     /pseudo
     CDS             complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="K0W65_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30355..31602)
                     /locus_tag="K0W65_000131"
     CDS             complement(30355..31602)
                     /locus_tag="K0W65_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBI3093567.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31624..31950)
                     /locus_tag="K0W65_000132"
     CDS             complement(31624..31950)
                     /locus_tag="K0W65_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBI3093568.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32169..33134)
                     /gene="glk"
                     /locus_tag="K0W65_000133"
     CDS             complement(32169..33134)
                     /gene="glk"
                     /locus_tag="K0W65_000133"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBI3093569.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33338..34594
                     /locus_tag="K0W65_000134"
     CDS             33338..34594
                     /locus_tag="K0W65_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBI3093570.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34709..35035
                     /locus_tag="K0W65_000135"
     CDS             34709..35035
                     /locus_tag="K0W65_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBI3093571.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35176..36414)
                     /locus_tag="K0W65_000136"
     CDS             complement(35176..36414)
                     /locus_tag="K0W65_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBI3093572.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36750..37952
                     /gene="nupC"
                     /locus_tag="K0W65_000137"
     CDS             36750..37952
                     /gene="nupC"
                     /locus_tag="K0W65_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBI3093573.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38002..40114)
                     /locus_tag="K0W65_000138"
                     /pseudo
     CDS             complement(38002..40114)
                     /locus_tag="K0W65_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40322..40397)
                     /locus_tag="K0W65_000139"
     tRNA            complement(40322..40397)
                     /locus_tag="K0W65_000139"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40362..40364),aa:Ala,seq:ggc)
     gene            complement(40437..40512)
                     /locus_tag="K0W65_000140"
     tRNA            complement(40437..40512)
                     /locus_tag="K0W65_000140"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40477..40479),aa:Ala,seq:ggc)
     gene            40733..41092
                     /locus_tag="K0W65_000141"
     CDS             40733..41092
                     /locus_tag="K0W65_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBI3093574.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41094..41486
                     /locus_tag="K0W65_000142"
     CDS             41094..41486
                     /locus_tag="K0W65_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBI3093575.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41538..42953)
                     /gene="gltX"
                     /locus_tag="K0W65_000143"
     CDS             complement(41538..42953)
                     /gene="gltX"
                     /locus_tag="K0W65_000143"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBI3093576.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43212..43287
                     /locus_tag="K0W65_000144"
     tRNA            43212..43287
                     /locus_tag="K0W65_000144"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43245..43247,aa:Val,seq:tac)
     gene            43332..43407
                     /locus_tag="K0W65_000145"
     tRNA            43332..43407
                     /locus_tag="K0W65_000145"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43365..43367,aa:Val,seq:tac)
     gene            43412..43487
                     /locus_tag="K0W65_000146"
     tRNA            43412..43487
                     /locus_tag="K0W65_000146"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43445..43447,aa:Lys,seq:ttt)
     gene            43518..43643
                     /locus_tag="K0W65_000147"
     ncRNA           43518..43643
                     /ncRNA_class="other"
                     /locus_tag="K0W65_000147"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43763..44104
                     /locus_tag="K0W65_000148"
     CDS             43763..44104
                     /locus_tag="K0W65_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HBI3093577.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44095..45021)
                     /locus_tag="K0W65_000149"
     CDS             complement(44095..45021)
                     /locus_tag="K0W65_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3093578.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45111..46109
                     /locus_tag="K0W65_000150"
     CDS             45111..46109
                     /locus_tag="K0W65_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBI3093579.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46106..46324)
                     /locus_tag="K0W65_000151"
     CDS             complement(46106..46324)
                     /locus_tag="K0W65_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HBI3093580.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46326..48341)
                     /gene="ligA"
                     /locus_tag="K0W65_000152"
     CDS             complement(46326..48341)
                     /gene="ligA"
                     /locus_tag="K0W65_000152"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBI3093581.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48412..49398)
                     /gene="zipA"
                     /locus_tag="K0W65_000153"
     CDS             complement(48412..49398)
                     /gene="zipA"
                     /locus_tag="K0W65_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBI3093582.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49628..50389
                     /gene="cysZ"
                     /locus_tag="K0W65_000154"
     CDS             49628..50389
                     /gene="cysZ"
                     /locus_tag="K0W65_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBI3093583.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50574..51545
                     /gene="cysK"
                     /locus_tag="K0W65_000155"
     CDS             50574..51545
                     /gene="cysK"
                     /locus_tag="K0W65_000155"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBI3093584.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51929..52186
                     /gene="ptsH"
                     /locus_tag="K0W65_000156"
     CDS             51929..52186
                     /gene="ptsH"
                     /locus_tag="K0W65_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBI3093585.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52231..53958
                     /gene="ptsI"
                     /locus_tag="K0W65_000157"
     CDS             52231..53958
                     /gene="ptsI"
                     /locus_tag="K0W65_000157"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBI3093586.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53999..54508
                     /gene="crr"
                     /locus_tag="K0W65_000158"
     CDS             53999..54508
                     /gene="crr"
                     /locus_tag="K0W65_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBI3093587.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54550..55401)
                     /gene="pdxK"
                     /locus_tag="K0W65_000159"
                     /gene_synonym="thiJ"
     CDS             complement(54550..55401)
                     /gene="pdxK"
                     /locus_tag="K0W65_000159"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBI3093588.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55506..55880
                     /locus_tag="K0W65_000160"
     CDS             55506..55880
                     /locus_tag="K0W65_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBI3093589.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55913..56636
                     /locus_tag="K0W65_000161"
                     /pseudo
     CDS             55913..56636
                     /locus_tag="K0W65_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56840..57751)
                     /gene="cysM"
                     /locus_tag="K0W65_000162"
     CDS             complement(56840..57751)
                     /gene="cysM"
                     /locus_tag="K0W65_000162"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBI3093590.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57885..58982)
                     /gene="cysA"
                     /locus_tag="K0W65_000163"
     CDS             complement(57885..58982)
                     /gene="cysA"
                     /locus_tag="K0W65_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBI3093591.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58972..59847)
                     /gene="cysW"
                     /locus_tag="K0W65_000164"
     CDS             complement(58972..59847)
                     /gene="cysW"
                     /locus_tag="K0W65_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBI3093592.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59847..60680)
                     /gene="cysT"
                     /locus_tag="K0W65_000165"
     CDS             complement(59847..60680)
                     /gene="cysT"
                     /locus_tag="K0W65_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBI3093593.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60680..61696)
                     /gene="cysP"
                     /locus_tag="K0W65_000166"
     CDS             complement(60680..61696)
                     /gene="cysP"
                     /locus_tag="K0W65_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HBI3093594.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(62000..62791)
                     /gene="ucpA"
                     /locus_tag="K0W65_000167"
     CDS             complement(62000..62791)
                     /gene="ucpA"
                     /locus_tag="K0W65_000167"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBI3093595.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62978..63778)
                     /gene="murR"
                     /locus_tag="K0W65_000168"
     CDS             complement(62978..63778)
                     /gene="murR"
                     /locus_tag="K0W65_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HBI3093596.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63942..64838
                     /gene="murQ"
                     /locus_tag="K0W65_000169"
     CDS             63942..64838
                     /gene="murQ"
                     /locus_tag="K0W65_000169"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBI3093597.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64842..66266
                     /gene="murP"
                     /locus_tag="K0W65_000170"
     CDS             64842..66266
                     /gene="murP"
                     /locus_tag="K0W65_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HBI3093598.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66446..67345)
                     /gene="yfeX"
                     /locus_tag="K0W65_000171"
     CDS             complement(66446..67345)
                     /gene="yfeX"
                     /locus_tag="K0W65_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBI3093599.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67441..68016)
                     /locus_tag="K0W65_000172"
     CDS             complement(67441..68016)
                     /locus_tag="K0W65_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBI3093600.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68077..68526)
                     /locus_tag="K0W65_000173"
     CDS             complement(68077..68526)
                     /locus_tag="K0W65_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBI3093601.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68513..68938)
                     /locus_tag="K0W65_000174"
     CDS             complement(68513..68938)
                     /locus_tag="K0W65_000174"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBI3093602.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69152..70021
                     /gene="amiA"
                     /locus_tag="K0W65_000175"
     CDS             69152..70021
                     /gene="amiA"
                     /locus_tag="K0W65_000175"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBI3093603.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            70025..70924
                     /gene="hemF"
                     /locus_tag="K0W65_000176"
     CDS             70025..70924
                     /gene="hemF"
                     /locus_tag="K0W65_000176"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBI3093604.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70930..71982)
                     /gene="eutR"
                     /locus_tag="K0W65_000177"
                     /pseudo
     CDS             complement(70930..71982)
                     /gene="eutR"
                     /locus_tag="K0W65_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(72028..72528)
                     /gene="eutK"
                     /locus_tag="K0W65_000178"
     CDS             complement(72028..72528)
                     /gene="eutK"
                     /locus_tag="K0W65_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBI3093605.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDIQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72541..73200)
                     /gene="eutL"
                     /locus_tag="K0W65_000179"
     CDS             complement(72541..73200)
                     /gene="eutL"
                     /locus_tag="K0W65_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBI3093606.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74382..75173
                     /locus_tag="K0W65_000180"
     CDS             74382..75173
                     /locus_tag="K0W65_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093607.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75893..76288
                     /locus_tag="K0W65_000181"
     CDS             75893..76288
                     /locus_tag="K0W65_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBI3093608.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76306..76569
                     /locus_tag="K0W65_000182"
     CDS             76306..76569
                     /locus_tag="K0W65_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093609.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76592..77134
                     /locus_tag="K0W65_000183"
     CDS             76592..77134
                     /locus_tag="K0W65_000183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093610.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77273..77560
                     /locus_tag="K0W65_000184"
     CDS             77273..77560
                     /locus_tag="K0W65_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093611.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77599..77805
                     /locus_tag="K0W65_000185"
     CDS             77599..77805
                     /locus_tag="K0W65_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093612.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78117..78602
                     /locus_tag="K0W65_000186"
     CDS             78117..78602
                     /locus_tag="K0W65_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBI3093613.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78583..78861
                     /locus_tag="K0W65_000187"
     CDS             78583..78861
                     /locus_tag="K0W65_000187"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093614.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78854..80314
                     /locus_tag="K0W65_000188"
     CDS             78854..80314
                     /locus_tag="K0W65_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI3093615.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80348..81157
                     /gene="murI"
                     /locus_tag="K0W65_000189"
     CDS             80348..81157
                     /gene="murI"
                     /locus_tag="K0W65_000189"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBI3093616.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81276..81970)
                     /locus_tag="K0W65_000190"
     CDS             complement(join(81276..81724,81724..81970))
                     /locus_tag="K0W65_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3093617.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82489..83862)
                     /gene="eutA"
                     /locus_tag="K0W65_000191"
     CDS             complement(82489..83862)
                     /gene="eutA"
                     /locus_tag="K0W65_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBI3093618.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83837..84316)
                     /gene="eutP"
                     /locus_tag="K0W65_000192"
     CDS             complement(83837..84316)
                     /gene="eutP"
                     /locus_tag="K0W65_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBI3093619.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(84329..84664)
                     /gene="eutS"
                     /locus_tag="K0W65_000193"
     CDS             complement(84329..84664)
                     /gene="eutS"
                     /locus_tag="K0W65_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HBI3093620.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(84957..87236)
                     /gene="maeB"
                     /locus_tag="K0W65_000194"
                     /pseudo
     CDS             complement(84957..87236)
                     /gene="maeB"
                     /locus_tag="K0W65_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
     gene            87525..88475
                     /gene="tal"
                     /locus_tag="K0W65_000195"
     CDS             87525..88475
                     /gene="tal"
                     /locus_tag="K0W65_000195"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBI3093621.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            88495..90498
                     /gene="tkt"
                     /locus_tag="K0W65_000196"
     CDS             88495..90498
                     /gene="tkt"
                     /locus_tag="K0W65_000196"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBI3093622.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(90601..91644)
                     /locus_tag="K0W65_000197"
     CDS             complement(90601..91644)
                     /locus_tag="K0W65_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBI3093623.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(91770..92345)
                     /gene="nudK"
                     /locus_tag="K0W65_000198"
     CDS             complement(91770..92345)
                     /gene="nudK"
                     /locus_tag="K0W65_000198"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBI3093624.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(92413..94392)
                     /gene="aegA"
                     /locus_tag="K0W65_000199"
     CDS             complement(92413..94392)
                     /gene="aegA"
                     /locus_tag="K0W65_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HBI3093625.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
BASE COUNT        24339 a        24089 c        22578 g        23495 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcat aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga caaaaagaag aatatgataa atattaattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttt aatccccttt
    22501 taaatttatg gtttttcctc aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 attcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttt aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
    25861 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
    25921 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
    25981 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
    26041 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
    26101 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
    26161 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
    26221 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
    26281 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
    26341 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
    26401 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
    26461 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
    26521 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
    26581 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
    26641 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
    26701 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
    26761 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
    26821 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
    26881 taatcggctt gtcgcccgcc gccagtagca cctgctggta ggcttcaaat tgctcctgct
    26941 cgtccggcgc gctgtcgcga tccatataga gcatttcggt acggaacagc ccgacacctt
    27001 ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt gccgatattc gccgcaatat
    27061 caatacgctt gttatcacgg gaataggcca gctgcgcggc agcctgcgcc tgttgttttt
    27121 ggcgtttatc cgccagcgtc tgcgcgacct gataataacc gctcaccgcg tcattcgggt
    27181 taatcgccag cacgccgcac tgggcgtcaa gcactgcagg ttgcccggca taacgggcaa
    27241 tcgcatccag cggcaagcca ctcagtaccg ggatcgccga ggcacgggcc agaatcagtg
    27301 tatgcgaggt gcgcccggtt ttctccagaa tcatacccgc aagatttttc aaatcaaggc
    27361 tcaaaaactg gcttggggtt aaatcttcag ccaccagaat ggtcggtttt tccagcacca
    27421 gattgttgcg cggcttcagt tccggccagg tgatatgcag caactgttcg ctgatatcgc
    27481 gaatgtcgct gacccgctcg cgcagataat cgctggcaga agcagatagt ttggcgcaaa
    27541 cctgctccat attgctgatg atcgccgccc ccagtccctg atgctgttct gtcatcaggc
    27601 gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgagcg ctgaggatag
    27661 ttttgctttc gccgtcacgc tcacgcaatt gctgattcag ttgctcggca agcgttgcca
    27721 ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gattgcccga taactgtcga
    27781 ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac gccgcttgcc agcacattgc
    27841 cgtacagtaa atccgggtta aggcggctta atgaacgcgg cagcggatgc gccgtcagtt
    27901 ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg caccaggatg tactcttcca
    27961 gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa ctgtcattaa
    28021 ataaggtgcc agttccaatc aacgccagcg agcttttagc atcagccttt gcgttctggc
    28081 gatggttgat aaaggtgatt tcgctttgcc actggctgca ctgttcttta agttcccacg
    28141 ccggacgagc atgtagaccg ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg
    28201 ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc tcacgcgtaa
    28261 gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg caatcggcaa
    28321 tcgatgccgc gcaatgtccg tggcgggtgg caggccccat taccaatgtg ggtacgccag
    28381 taccggttaa gtgcaccgct ccaccgtccg tgccgccgtt actgaacata tccgcctgca
    28441 acggaacgcc aatttctgcc gccacggttt caatccacgc ggtaagtttt ggcggcgcaa
    28501 tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga tggttagccg
    28561 cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg acgtccggcg
    28621 acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac ctcttcgctg gaacttgcca
    28681 ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgtagtaac gtcaccagca
    28741 gatagcaact gaggcgatca tcaaaggctt tgcccatcac ccgctggtga gggaggacct
    28801 gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat tcccgcctgc atcacttcgt
    28861 cataggtgcg cgcgccaatg tccacgcgca tggcgctaac gtcattcccc tgccggtcgc
    28921 cgtcaagcag gcctggaatt ttgcactctt cacgggtggt gatgcgcacc ggctgcagct
    28981 ggcgggcagc catgcgcacg ttgccaaccg gcagcacgtc aatagcgcct tcgcgggaga
    29041 tgctgcgcac cataaatccc acttcgtcca tatgggcgca gatcatcacc ttcggacctg
    29101 tcgattcatt gaggcggatc agcaccgatc ccagaccatc aaatcgcact tctttttgca
    29161 ggcgagccgc ttcttccagc aggatctgcc gcacttcctg ttccgaggag gcgatcgcat
    29221 ctgcctcgct caacgctttt aatagcgata aatccattat gcctctcccg tgagcaacac
    29281 tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtaaccagca caacatcttc
    29341 gatgcgcacg cccccttgcc ctagcaaata aatccccggc tccacggtca gcaacatgcc
    29401 tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga tcttcatgga cttcaatgcc
    29461 gatagcgtga ccagtgttat gaccgaaata gtcgccaaat ccagcctcgg taatgacccg
    29521 gcgcgcggcg tcgtcaatct gctggcagcg cacgccgggg cgaattgcag agattgccgc
    29581 gagctgtgcc tgcaggacaa tctgatagac gtcaaacagc gggtgagatt cggcgctcac
    29641 cccttcgcca ttcaccagca aggtgcgcgt catatcagca gtagccctga tacagcgcac
    29701 caaaatcgag agtgacaaac tcgcccgctg caacaatctt gtcgctggct ttgccgtgcg
    29761 gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa agaggctttt tctgcgccct
    29821 gctggcgcat aaaccactcc agttcagcgg ctatctcgcg ctcgctcatc cccgcctgaa
    29881 taaagcggcg aatatgctcc gctccgcgat cggcaatccc acaggcgagg cggattatct
    29941 ccacctcctc tggcgttttg atttgccgca gcacatccgg cgtggcgcta accagtttcg
    30001 cattgagttc agacttccag cggtgcgcgg tttcccaact cacctgctgg ccttcaaaac
    30061 cgagcgtttg caactgctca tcggcaatga tttgattgac gatagtggta agcgtgttcg
    30121 ttgcgtcaag caaatgcagc tggtagcctt gcgcacgggc ttccacctca acgaaatagc
    30181 gcgaatctac cagaatgtgc gcactttcac ggctaataac cacatagcct gatccggtgg
    30241 agatccccag atgcggctgt ttgttctgcc gtgaggagag aagcaccgca tccagttgtt
    30301 gcaccttaag ccagttgcgc agcgaagcga gtaatgtcat ctggattttc cttcttacag
    30361 gctatcgatt aacaatttgc ctttgcggaa catcatcaga cgcaggaaca ccaccatcag
    30421 cgcagtaatg attgccccca gcgcgatccc cgccatataa acgcccaagt tagtgaccag
    30481 cggccatgcc cagatagcgg attccgggaa ccattgcact gcgcccagcc agacagctgc
    30541 ggttgagccg acaatcgcgc cgaccatata tgacggaata gcggtgatgg ggctttccag
    30601 cgcaaacgga atcgcaccct cactaatccc cataaaggcc aggaacatcg cggtcttacc
    30661 ctgcggataa agttgagcgt tgaacaggcg tttgccggtt aaacgacggt caataatggt
    30721 cgccagaccc agaccaatcg gcggaataac gatagcgata gaacgagcgg tgaccggtaa
    30781 tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg gctttgttga tcgggccacc
    30841 aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc ccttctcacc
    30901 tgcggcagtc agcacagtac ggataccgcc gttgatccag ccaccaaagg gagtgatgac
    30961 gtagtacatc gccagcatga caaaaatggc ggaaagaatc ggtagcaaaa atgtggtttt
    31021 gaacgctaac aggaaatccg gtaactggat tttctggttc atccatttca caaggtaacc
    31081 ggcaacgata gaaataatca gcgcaccaat gaaggtggac ggcaccggcg aagaggtcgc
    31141 ccattgcata gtgctgggat cgaagttcag cagttgcgtc ggctgggtag acatcaaccc
    31201 gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca caaatgcagc
    31261 aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca gccaggcaaa
    31321 tttaagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat ccatgatgcc
    31381 gatctcagca ggaattttaa gccagctata agcaatcagc tggctgaaag cgaggatcac
    31441 accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat gctgcgggag
    31501 ttcaccccag aagctgcttt tagaggcctg cggattctta actgccgctg ccgcaccgga
    31561 tgcgccaggc acaacggttg cactgcgttt tttaatggcc ataacaatta tccctgtcga
    31621 atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgatgc ccgcagcgtt
    31681 tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac gttcgttatc
    31741 ttccggggta actgccacgg aatggatgat gatggtcgct tcggcgatat cctgcgccgt
    31801 caggcgattc tggatgccgt ccgcgccctg ggtttcaatt ttcacttcat aaccggcttc
    31861 taccgccgct tcttccagca cctgagcggc cataaaagtg tgagccaggc ccattgggca
    31921 ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg atagttgact
    31981 agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag acaaataatg catttactgg
    32041 agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg gacacaaaga
    32101 aaaattgaat taactgtatg atttaaaaga ttatcgggag agttacctcc cgatataaca
    32161 ggaaggattt acagaatgtg acctaaggtc tggcgtaaat gtgcaccgga accgagaaga
    32221 cccggatagt catggacgat gagatacacc ggaatatcat ggacatattc tttaaagcgc
    32281 cctttatctt caaatgcggc acggaaaccg gaggctttga agaactcaag gaagcgcggc
    32341 acgataccgc ccgcaataaa cacgccgcca aatgtcccga gattgagcgc cagattgccg
    32401 ccaaaacggc ccataatgac gcaaaacagc gacaatgcgc ggcggcaatc ggtgcagctg
    32461 tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag gcggttgtca
    32521 gctttcacaa ttgcgcgata caaattcacc agcccagggc cagaaagcac gcgctccgcc
    32581 gaaacatgac caatttccgc acgcaatatt tcgaggataa tggcctcttc ttcactattc
    32641 ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta cccaacgctt atcgacatgg
    32701 accagatgcg caaccccaag ccccgttccg gcaccgtaaa ccgcaatagg cttaccttcg
    32761 accggttctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg gatcgccatc
    32821 gatacagcgg taaaatcgtt aataatttcc agatggctaa aaccgagatt ctttttcatt
    32881 tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc ggtaattggg
    32941 caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag ataaacgcga
    33001 atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg cgagatttca
    33061 ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc accgactaat
    33121 gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt ctcacactgt
    33181 aaatacctgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa tatcgatctg
    33241 ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt tgcaagctgg
    33301 gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc gagccagaac
    33361 catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc ttattctgat
    33421 tgtggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac tgccgggaac
    33481 tctggggata gcccaggatt cacccctctg gatcatcggt gtattaacgc taacgggtat
    33541 tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagacc ccgcctgtga
    33601 accgctgatc ggcgcaccgg ttccgccctc tgcgctacct agacttatcg tagcattaat
    33661 tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcatga ccgtcaatat
    33721 cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc gtcaaccgaa tggagtggac
    33781 tattttagcc tctgccggaa ccatcggcgc gctgtttggt acacctgttg cggcggcgtt
    33841 gatattttcg caaaccttaa atggcagtag tgaagttccg ctatgggatc gtctttttgc
    33901 gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc atccccattt
    33961 ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca gcggtgcaat
    34021 tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac cacggttgca
    34081 cgcgatgatg aatcaaatga aaaatccggt gctcgtgctg ggtattggcg gatttattct
    34141 cggaattctg ggggttattg gtggaccagt ttcgctgttt aaagggctgg atgagatgca
    34201 gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg cggtaattaa
    34261 acttgccgcc ctggtcgttg ctgccgccag tggctttcgc ggtgggcgaa tcttcccggc
    34321 agtgtttgtc ggcgtggcat tagggttgat gctgcatgag cacgttcccg ccgttccagc
    34381 ggcaataacc gtttcctgcg ctattctcgg catcgtgctg gtggtaacac gcgatggctg
    34441 gttaagtctt tttatggcgg cagtcgttgt acccaatacc acattgctac cgctgctctg
    34501 tatcgtcatg cttccggcat ggctgttatt agcaggtaag ccgatgatga tggtcaatcg
    34561 tccgaagcaa cagccacccc acgataatgt ttagcaaaaa atgctctcct ttatgattta
    34621 agagttatgg cgatgatacg gggctttacc tccccgtaat attgcgttaa caggccgctg
    34681 acggcttcga tcgtgaagga gaataacaat gttcaggtca ctgtttctgg cggccgccct
    34741 gatggcattt accccgcttg cagcaaatgc aggtgaaatc accctactgc catcgatcaa
    34801 attacaaatt ggcgatcgcg atcattacgg taattactgg gacggtggtc actggcgaga
    34861 tcgtgattac tggcatcgca attatgaatg gcgcaaaaac cgctggtggc gtcatgataa
    34921 tggctatcat cgtggctggg ataagcgtaa agcatatgag cgtggctatc gtgaaggctg
    34981 gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat gggcaccgcc attaaacgcg
    35041 tcctccaatg gagcacaatg cctgatgcga cgctagcgcg tcttatcagg cctacaaacc
    35101 ggctctttaa cgcgtagggc ggataaggcg tttacgccgc atccgccaat agtgccagat
    35161 gcgacgctca ttcaactaca atcccagcgc cgtccccacc aacaaccaga tattcagcgc
    35221 cacgaccagc actacaatca cccagcctgt ctgttttacg cgtttgctgt tcaccagatc
    35281 gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga accagcgcca gggcgatacc
    35341 aaaacttaac agcacctgac tcataaccag aatccgtgtc ggatctaatc ccatcagaat
    35401 gacaataaat gacggcaaca tggtgactgt acgacgcacc cacagcggga tatggaagcg
    35461 aatgaatccc tgcatcacca cctgccccgc cagtgtcccc accaccgttg aggacagtcc
    35521 ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca gcggttgcag
    35581 cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt ggaacgccgc
    35641 cgcagctgta gccatcatca ccagattgac aaaaccggca atcgtcatgg cgatagccac
    35701 atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat gctgagtgag
    35761 cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc ctgctgccag
    35821 gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt cctttaccca gctgcgccag
    35881 gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca acagtaaccc
    35941 gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa tcaggaaagt
    36001 cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga gtttaaaacc
    36061 gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt ctgcctgaac
    36121 ccaatagaac cacactacgg gacgcggata gtgatcacga atctgctccg ccagattttt
    36181 accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca tcaggttggc
    36241 ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa tattggtcgc
    36301 aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca ttaatgcgag
    36361 cctcatctta cgcgccgccc gtccgctgct actctcaacg cgatagttcg tcatcttgtg
    36421 cctctaaaac atagcctttg ctatgtttca tgctatgcta aacgagaatg attatcaaat
    36481 tcatttaaat ggattgtggt gatttctctg atagaccaag attatgatta cgaaaagatt
    36541 gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact ttcagcttcc
    36601 atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt cacaggacgt
    36661 cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat gtgcacggaa
    36721 atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt gtactggcac
    36781 ttgccgttgt tgtgattctc gcactgctgg taagcagcga ccgcaaaaaa attcgtatcc
    36841 gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc ctgaactccg
    36901 acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg ctcggatttg
    36961 ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg gcattcttct
    37021 tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt ctccagcaca
    37081 ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa gtcaacggta
    37141 tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag tcagaaaact
    37201 ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac accatggctg
    37261 ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc atgctggaac
    37321 cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc gtgctgtcgc
    37381 tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc aacctgcacg
    37441 aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc aaagttgcca
    37501 ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcgctg aacgcactgt
    37561 ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc ctgggctaca
    37621 tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg caagtgggca
    37681 gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat ctgcagaaaa
    37741 ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttccta gtttccttcg
    37801 ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat gaagagcaag
    37861 gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg gtgagtgtgc
    37921 tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc cggggatcat
    37981 tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa tcaaataccc
    38041 ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa
    38101 ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc tcacaatcat
    38161 cgcatccagc gtattcgtga caatatcttt cacaaagacg ccatcaattt tgatgatatc
    38221 agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc
    38281 aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt ctgcgttaga
    38341 aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc ggggagatgt gataacgctt
    38401 aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg gcattaaatt
    38461 gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact tcaacaagga
    38521 ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa
    38581 cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct catcataacc
    38641 ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct gatctaacgc
    38701 cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa ccatctcctc
    38761 tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca gccagcttaa
    38821 ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc aggcagcacc
    38881 atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca ggatgttaac
    38941 catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca gttcactacc
    39001 cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac ggcaaattga
    39061 gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact caagattatc
    39121 aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc ggcgcttgtt ccaacgcacg
    39181 aaagttgggt aggagcgtta acggatctgt tagcgcctgc aaatgccagc ggcgattaag
    39241 ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa aggaaatcag
    39301 caccgcgaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa agttctgatt
    39361 gtaattcaga aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag
    39421 attaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaggtaac cggcaataaa
    39481 gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg ccagccagct
    39541 taaggtaaat gcgagtgaga tagtagaaaa gcatgttgta aatcagcact gcggtgaaaa
    39601 ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag gtggatatct
    39661 ttagcgggaa atcaaagaag ctccccacaa gatacatgct gcatttgatg ccgatcggcg
    39721 tcaccaaacc aagccagaac agacgttgcc agacatatcg gctggtcaat ccataacgcc
    39781 aacgcgtccc cagctgccag cgtaatattg cgcaagcaca caacacgaca aacgtctggc
    39841 tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa gttccacaga ttggtgcaaa
    39901 acatcccgca taaaacggga acgacaccgc gccagccaaa gataaacaag attgacagca
    39961 ttacgcacag tggcatccac gccagaaaga tataactgga atggataatg gcaagtggcg
    40021 aaataaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt gtgaatatct
    40081 tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca ctgatcgggg
    40141 ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg gccagcctgt
    40201 tgccgatttt tcatccaatg atgctttcac caggcggcgc taacccggac gtagactgta
    40261 ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct ttgcagcgag ggttggaaat
    40321 ttggtggagc taagcgggat cgaaccgctg acctcttgca tgccatgcaa gcgctctccc
    40381 agctgagcta tagccccacg atgcgtttac gtaccaaatt tgctgggtgc aaaatttggt
    40441 ggagctaagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg
    40501 agctatagcc ccgtcacgta aagcttgtcg agttgacggg cggcatcata tgaattccgc
    40561 ccgaatgtgt caacggcaaa ttgcaacgtg tagtttcaat cgctgaaaaa tcaggcaaat
    40621 gaacaatttt ggaaaaccgc tcgcattcag tagttattca tgtcacggtt tcctgtaaag
    40681 tggtgttata aaatgaacta ctaatagacc cacatacatt cagggaattg ttatgttcaa
    40741 ggagaggatg acaccagatg aacttgccag actgaccggc tatagccgcc agaccattaa
    40801 taaatgggta cgcaaggaag gctggacaac gtcaccaaaa cctggcgtcc agggtggcaa
    40861 agcccgactg gttcacgtca atgaacaggt tcgtgaatat attcgcaatg ctgaacgtcc
    40921 agaaggtcag ggagaagcgc ctgccctttc tggtgatgcg ccgcttgaag tattgctggt
    40981 gacactggcg aaagagatga cgccagttga acaaaaacag tttacatcct tgcttctgcg
    41041 ggaagggatt atcggattgt tacaacgctt agggattcgc gatagcaaat aatatgaaaa
    41101 gattacgcaa taaaatgacc accgaagaac tggctgaatg cctcggtgtg gctaaacaaa
    41161 ctgttaaccg ttggaccaga gaaaaaggct ggaaaacgga aaaatttccc ggcgtgaaag
    41221 gcggtcgtgc cagacttatt ctggtcgata cgcaagtttg cgagtttatt cagaacacgc
    41281 cagccttcca taacacacca atgttgatgg aagctgaaga gagaattgct gaatacgctc
    41341 ctggagcacg cgccccggcg tatcgacaaa tcatcaacgc gatagataat atgacagaca
    41401 ttgagcagga aaaagtcgcg caatttttgt cacgtgaagg aattcgcaat ttcctcgccc
    41461 gtctcgatat tgacgaatca gcataaataa aaaacggcag gatattatct cctgccgttt
    41521 atctttttac acgctaatta ctgctgattt tcgcgttcag caataaaatc cagcgctttg
    41581 ttgatacgct cgatactgcg ggtcttacca attgcgtgaa cggtaacatc cagtgctgga
    41641 gactgccccg caccggttac ggcgacacgc agcggcatac caactttacc catacccact
    41701 tccagctcat ccgccgtcgc ctgaatagcg tgatgaacgt tttcagcggt ccagtcagta
    41761 atcgcggcca gtttgtcacg aaccacttcc agcggctgac gcgctaccgg acgcagatgt
    41821 tttttcgccg catcggcatc gaactcagca aaatcttcgt agaaataacg gcagctctgt
    41881 gccatctctt tcagcgtctt gcagcgttcg cccagcagtt tcaccagatc agccagctgc
    41941 gggccgttac gggtatcgat attttcctgc tcaatgtgcc actgtaagtg agtagcaaca
    42001 tactccggcg gcagcgcgtt aatgtagtga tggttcagcc acagcagctt gtcggtgttg
    42061 aacgcactgg cagatttgct gacggcattc aaagtgaagt atttgatcat ctcttcacga
    42121 gtgaagattt cctgatcgcc gtgggaccag cccagacgca ccagatagtt cagcagtgct
    42181 tctggcaaat aaccgtcatc acgatactgc attacgctga ctgccccgtg acgtttggac
    42241 agttttttac cgtcatcgcc attgatcata gaaacgtgcg cgtaaaccgg caccggcgct
    42301 ttcagggcct taagaatgtt gatctggcgc ggcgtgttgt tgatatggtc ttcgccacgg
    42361 ataacgtggg tgatttccat atcccagtca tcgacaacca cacagaagtt ataggttggg
    42421 gaaccatcgg tacggcggat aataagatcg tccagttcct ggttgctgaa ctcgatcgga
    42481 ccacggatct gatcgtcaaa aacaacagaa ccttcctgcg ggttagcaaa acgtacaaca
    42541 cacggttcat catcagcatg atgctcatgg ctgtggcggc agcgaccgtc ataacgcggc
    42601 ttctcacctt tcgccatttg ctcttcgcgc agcgcttcca ggcgctcttt agagcaatag
    42661 catttataag cagtgccctc ttccagcatc tgatcgatca ccgcgttgta gcgatcaaaa
    42721 cgtttggtct ggtagtacgg accttcatcc cactccaggc tcagccagtt catgccatcc
    42781 ataatggctt cgatagcttc cggcgtggaa cgctcaagat cggtgtcttc aatacgcagc
    42841 acgaactcac cgccgtggtt acgtgcaaaa agccaggagt aaagagcagt acgcgcgccg
    42901 ccaacgtgca gatagcctgt tgggcttggc gcgaagcgag ttttgatttt catgaaatgg
    42961 ccttacgttt agaaagatgc cgacaaccgg caaatcctgg aaaaattgag tgggcgatat
    43021 tctatcactc ccgcctgatt cctcaatgta gttcgggttt ctaccttacg cattattgtt
    43081 ttcgttgaca aattgcgcaa cacggtctgt tttgcgatca tcttgcttaa atttacgacg
    43141 aacgaacaat ttctttagaa aatgcgttga ctcattttga actctcccta taatgcgact
    43201 ccacacagcg ggggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    43261 gcggttcgat cccgtcatca cccaccaact actttatgta gtctccgccg tgtagcaaga
    43321 aattgagaag tgggtgatta gctcagctgg gagagcacct cccttacaag gagggggtcg
    43381 gcggttcgat cccgtcatca cccaccactt cgggtcgtta gctcagttgg tagagcagtt
    43441 gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccaatg taaaaaagcg
    43501 ccctaaaggc gcttttttgc tatctgcgat actcaaagat tcgaacctgc agcaggtttg
    43561 agttgagcgc agcgaaacaa cggagccgct cgcggcgacg gcccgaaggg cgagcgaagc
    43621 gagtcatcct gcacgaccca ccaatgcaaa aaagcgccct aaaggcgctt ttttgctatc
    43681 tgcgatttgt aagattgatt tttcttctca agttccttgt tcttctaccg atattacctc
    43741 gtacagtgac gaggactgaa caatggccac tatctcttca acttcaattc cttccataca
    43801 aacacagagt agtaacaggg catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat
    43861 aagtcagcaa atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag
    43921 tgctgaagac aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac
    43981 acaactggcg cagttgcaga aacagcaggc agaaaaggcg caggagaaag agcagcgttt
    44041 gtctctgaat gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat
    44101 ctgatacaac ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac
    44161 gtcccataac gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
    44221 ctggcgttca accaccggcg taatgcgctt aaccaccagt gggctacctt caggcaatgg
    44281 tagcgccagt gcaggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
    44341 cgccggatgt ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc
    44401 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
    44461 tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
    44521 gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac
    44581 gatgccaaag tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
    44641 atgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
    44701 aagatgcgcg gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc
    44761 catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa
    44821 agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
    44881 aacaccagta tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc
    44941 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
    45001 ttgttttaaa gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat
    45061 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
    45121 ttcgtatcct cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct
    45181 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc
    45241 tgctgttctt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc
    45301 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg
    45361 tactgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
    45421 acttgtgcat tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
    45481 gtaacgtcgc ggcggcggtt tgttctgcgt cagcatccag cctgctgggg attttccttt
    45541 caccattgct ggttggtctg gtgatgaatg ttcacggtgc aaggggcagc cttgagcagg
    45601 tcggtaaaat tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt
    45661 ggattggtga ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca
    45721 ttctgttggt ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag
    45781 ttggctgggg atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga
    45841 ttgtagttaa cgtctttatg gcacgccgac tgagcttcaa taaggcagat gaaattacta
    45901 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
    45961 tccccacatc ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat
    46021 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc
    46081 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
    46141 tcagcccctc ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg
    46201 ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtca attaagcgac
    46261 gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
    46321 ccatctcagc tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt
    46381 tcctgcgcct tcgccagttt agatcctgca gcttcacccg ctatcaccag atcggttttc
    46441 ttcgacacgc tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca
    46501 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca
    46561 atctcttccg cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg
    46621 ctgatgacat tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca
    46681 atgccaacat caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc
    46741 gtgccgaaat atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt
    46801 gcatagagga agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
    46861 tgtgccaatt ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
    46921 agatctgccg gagtatggac atattctttt tcaaccagct gatcgatgat tttgtcgccc
    46981 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc
    47041 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg
    47101 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
    47161 tcagaaagca cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact
    47221 ttatcgccaa tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta
    47281 ctcaccagca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
    47341 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat
    47401 ttaaacgcaa ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc
    47461 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct
    47521 tccactttgt gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg
    47581 ctgaccggca ccccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
    47641 tcgccacctt ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta
    47701 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact
    47761 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
    47821 acttcaccac gcacttccag acgcgccggg atattctctc cgtgcagctt cagcggaatg
    47881 gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc
    47941 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc
    48001 agctcacagc accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg
    48061 aaagcaagaa agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg
    48121 cgtatctggc tgaaagctgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc
    48181 gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
    48241 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcaaagc
    48301 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
    48361 aaacccccga caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcaggcgtt
    48421 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
    48481 acggcgctga tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg
    48541 cagcatcagc ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt
    48601 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
    48661 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt
    48721 catatcgcca aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag
    48781 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt
    48841 atccataact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
    48901 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc
    48961 gacctgtggc gaaactggct gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg
    49021 ctgcaccggc tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg
    49081 ctggaccggt tgacgcggct gcgcagacgc ataaggcggt aggtactggt gttgcggcga
    49141 cggacgagca gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg
    49201 aacctcacca acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt
    49261 tgacttcatt cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt
    49321 ccagaaacca tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag
    49381 acgcaaatcc tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca
    49441 tttactcact aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca
    49501 gcacaaagat agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca
    49561 ttgcgcaatt acccggtaaa atacgcagaa ttttcctggg attggtcaaa aggagctcat
    49621 cctgattatg gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca
    49681 aggctggaag ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt
    49741 caatattttg ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat
    49801 cccgactttc atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc
    49861 tctggctgtc atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg
    49921 gattgccgct ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc
    49981 tacaccgcca gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg
    50041 cgaatggcaa aaatttgtct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt
    50101 catccctggt attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat
    50161 gttagccatc cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat
    50221 gcgcaccgcc ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct
    50281 gtttacgatg atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcgac
    50341 ggcgatgtgg gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg
    50401 ttattttgta aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt
    50461 gcatgtcatt atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc
    50521 tgagcgggta tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta
    50581 agatttttga agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca
    50641 tcggtaacgg acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt
    50701 gccgtatcgg tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg
    50761 ttgaactggt tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg
    50821 ctcgcggtta caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc
    50881 tgctgaaagc gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg
    50941 caatccaaaa agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac
    51001 aattcagcaa tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg
    51061 aagataccga cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga
    51121 ctggcgtcag ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg
    51181 ttgagccaac cgattctcca gttatcgccc aggtgctggc aggtgaagag attaaacctg
    51241 gcccgcataa aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc
    51301 tggtcgataa agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa
    51361 tggaagaaga aggtattctt gcaggtatct cttctggagc agctgttgcc gcagcgttga
    51421 aactacaaga agatgaaagc tttaccaaca agaatattgt ggttattcta ccatcatcgg
    51481 gtgagcgtta tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac
    51541 agtaatgcca gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc
    51601 ctgcttcaaa ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc
    51661 agactaatta ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat
    51721 tttatgattt ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc
    51781 agcggtcaac acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctgttaa
    51841 aaactggcgc taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa
    51901 cactaaacct ataagttggg gaaatacaat gttccagcaa gaagttacca ttaccgctcc
    51961 gaacggtctg cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc
    52021 tgaaattact gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca
    52081 gactctgggc ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca
    52141 gaaagcggtt gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt
    52201 ttaaaaatca gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt
    52261 atcgctttcg gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa
    52321 atttctgccg accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca
    52381 tcagcccagc tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa
    52441 gccatctttg aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata
    52501 gccctgatta aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt
    52561 caggcttctg ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta
    52621 cgtgatatcg gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc
    52681 gccattcagg atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag
    52741 ctgaacctga agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc
    52801 tctatcatgg cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct
    52861 caggtgaaaa atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat
    52921 ccaaccaacg aagttattga taaaatgcgc gctgttcagg agcaagtggc ttctgaaaaa
    52981 gcagagcttg ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa
    53041 gtatgcgcta acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa
    53101 ggcgttggtc tgtatcgtac tgagttcctg ttcatggacc gcgacgcgct gcccactgaa
    53161 gaagaacagt ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc
    53221 gttcgtacca tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa
    53281 gagaacccgt tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg
    53341 cgcgatcagc tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc
    53401 ccgatgatca tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa
    53461 caggaactgc gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg
    53521 gaaacaccgg ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt
    53581 atcggcacca atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt
    53641 tcacaccttt accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat
    53701 gcttctcatg ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt
    53761 gctacacttc tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc
    53821 ccgcgcatta agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag
    53881 caggctcttg ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa
    53941 gaaaaaacaa tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat
    54001 gggtttgttc gataaactga aatctctggt ttccgacgac aaaaaggata ccggaactat
    54061 tgagatcatt gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt
    54121 ttttgcggaa aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt
    54181 cgcgcctgta gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga
    54241 atctgatagc ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg
    54301 cgaaggcttc aagcgtattg ctgaagaagg tcagcgcgtg aaagttggcg atactgtcat
    54361 tgaatttgat ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat
    54421 ctccaacatg gacgaaatca aagaactgat caaactgtcc ggtagcgtaa ccgtgggtga
    54481 aaccccggtt atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgcccatcgg
    54541 cgccattttt tatgcttccg ccagcggcgg caaaatcaat tcatcgctct catgctgctg
    54601 ggtgtagcgc atcacttcca gtacgcgcaa ccccgcacgg tgcaccgcat cgtttaacgc
    54661 cttccctttc agcaaaccac tgataagctg agcacaaaac aggtcgccag tccctttcag
    54721 gtcggttttt acccgtgaat gggaaatgac attcacgctg tcggcactga ccaccaccac
    54781 ctgcatctcc tgattttctt cattaccgga ggcgctggta atcaccaccc attttaatgt
    54841 gtctgaaagc agactttttg cggcagcaat ggcactgtcg agatcgcggc aatttttacc
    54901 ggtcaggatt tccagctcaa agatattggg ggtaattccc tgcgccagcg gcagtaaata
    54961 ttgtcgatac gcctcgggaa ggtcaggttt gacataaatt ccgctatcaa tatcgccaat
    55021 caccggatcg accatgatca ataggtcagg atggtctttg cgtagcgcag tcagccactc
    55081 ggcaaggatt ttgatttgcg atgccgttcc catatagccc gtggttacag cacgaagttg
    55141 gcgcagcgca tcacgctcct gaagcgcacg caaatagccg ctaaaccatt cgtccggaat
    55201 cgcaccaccg tagaaagtgt cataatgcgg cgtattgctc agcaataccg tcggcacggc
    55261 aaagacattc aagccgttct gttttatagc aggcaccgca atgctgttgc ctacgcttcc
    55321 gtacactacc tgcgactgca cggcgacgat atccgcctgc agcgccctac tcttatcgtt
    55381 aaacaacaac aaactactca tttaattttt tctccttgcc gatgatcctc atcgtattcc
    55441 aaccgaaact ttacctgatt ctggcagtca aatcggctat tacaaaacaa agataaggta
    55501 attcagtgaa gaaaatcatt tgtctggcca ttacgctgct aatgacactc cccgcttacg
    55561 cgaagttaac tgttcatgaa gaagcccgca tcaacgccat gctggaggga ttagcacaga
    55621 aaaaggattt gatatttgtg cgcaacggtg atgaacatac ctgcgatgaa gcggtttccc
    55681 acctgcgtct gaagctcggc aatacccgta accgcattga cactgccgag cagtttattg
    55741 ataaggtagc ttcgtcgtca tcgattaccg ggaagccgta tatcgtgaag atccccggta
    55801 agagcgatga gaacgcacag ccttttttac atgcgttaat tgcgcagacg gataaaacgg
    55861 tgcctgcgga aggaaattaa tccgctttgg taaggcattt acaggaggta acatgaaaaa
    55921 atgctttatt tatcacgatg aaaaatcgaa taaattttgg tggatagatt acgaagggga
    55981 tagtttagct gtcaactatg gcaaggtagg tagtattggt aaattccata caaaagaatt
    56041 cgataatgaa gaacagtgtc tgaaagaagc cagtaaattg attgccgcaa aaatgaaaaa
    56101 aggatatcaa gaaactccag actttaactt catggatctc tactattttg atgatgaaga
    56161 gattgggtta catcttaaaa cgtcacaccc aaacttccag tgccatttta ctgatccact
    56221 ttatatgtgt tgctgggatg aagaatctcc ttttggtagc gatgaaggtg ctgatgctct
    56281 aaacgaactt gaaaatagcc tccgttctgg actgtgctga tttccctcaa atgttaattg
    56341 aaactatgtg gggtatgaaa tacatcgcta tggacagtat tcttgaagag gatgtgcgtg
    56401 cgcaattact agccgatgaa atgagctcta tccagagcaa tatgattacc tacgcgactg
    56461 cattcggtca gatcaaagtc atgggtaaaa tctcccataa acttaaaaag atgggactca
    56521 atgcactagc gcgtcatcag cttaccgcaa aaattcttca atggggtgac ggtcaggact
    56581 caccaatact tcaaaaaatg attgatgacc ttactgcttt tccacacgaa aattaaaaac
    56641 tgcatttgtc ggcagcaaca actgccgaca aatcagatta ttgacaaagt gcactttgtt
    56701 tatgccggat gcggcgtaaa cgcctgatcc ggcataaaaa tattgctaat tcaatatatt
    56761 gcagaaacat tgtaggcctg ataagcggag cgcatcaggc agttttgcgt ttgtcatcag
    56821 tctccaatgc tattaatcct taaatccctg ccccctgact aaaatgctct tccccaaaca
    56881 ctccggtgga gaggtagcga tcgccacgat cgcagatgat cgctaccacc accgcaccag
    56941 ggttagcttt tgccacccgc agtgctccag caaccgcgcc gcctgagctg acgccacaga
    57001 atattccttc ccgcaccgcc agttcgcgca tggtgttttc cgcatcgcgc tgatgaatat
    57061 ccagcacctc atccaccagc gcagcgttga aaatccccgg cagatattcc gcaggccagc
    57121 ggcgaatgcc cggaatactg ctgccttctt ccggttgcag gccaacaatg gtcaccggtt
    57181 tggattgttc gcgcataaag catgaaacgc cggtgatagt gccggtcgtc cccatgctgg
    57241 agacaaaatg agtgatgcgc ccgccggttt gctgccagat ttccggcccg gtggtggtgt
    57301 aatgcgcata agggttatcg ggattattga actgatcgag cagctttcct tcgccacgat
    57361 tcgccatctc cagcgccaga tcgcgcgcac cttccatgcc ctgctctttg gtgacaagaa
    57421 tcagttccgc accataagca cgcatcgccg cacggcgttc ctggctcatg ttgtcgggca
    57481 tcagcaattt catgcgatag cctttcagcg cggcaatcat tgccagcgca atgccggtgt
    57541 taccactggt ggcttcgatt aagacatcac ccggtttaat ttccccgcgc ttttccgcct
    57601 cgacgatcat cgaaagtgcc gcacgatctt tcaccgaacc tgccgggtta ttgccttcca
    57661 gttttaacca cacttcactg ccgttatccg gccccattcg ctgcaacttc accagaggcg
    57721 tattgcctat tgtttgttct aatgtactca caatctctat ccatacgtgg tgttgcctga
    57781 tgcgacgctt gcgcgtctta tcaggcctac aggttacaaa ccttgccatt aaaaagcccg
    57841 gatcgcggga agcgcctccg ggcgtttaac attcactcaa cctatcaggc gctttgtgcg
    57901 agagcaagtt cctcatcgcg ggtttcaata cgctcgtcgc cgttatacag ccgcgcatgt
    57961 tgcagaccaa cgaataaacg ctcgccatgc tgcggggcat cgtcgccatg catcacgacc
    58021 gtcagcggtt cgttgtacca ccccagcggc tgcaccacta attgggtgta gtgacctttc
    58081 gggctggctt ccagtacctg taccggcagc ggcgaatcga gactggtacg gcggctgata
    58141 tccacttccc acgggcgcag gaagagatcc accggcccct gatacgcagg tgtgtagccc
    58201 agcggccagc gatgcgcgcc aacatggaac tgcccgccgc gaatggttcc ctgcaggcgg
    58261 ttcacttcgc ccataaattc gagtacaaaa cgggtcgctg gttcgcgcca tacctgattc
    58321 ggcgcatcag cctgttcaat attcccctga ctcatcacca ctacacgatc ggctacttcg
    58381 gtcgcctctt cctggtcgtg ggtcacaaac acgctggtga atttcagctc ttcatgcagt
    58441 tgacgcagcc agcgacgcag ctctttacgc acctgcgcat ccagcgcgcc gaacggttca
    58501 tcaagcagca gaatttgcgg ttctacagcc agcgcgcgcg ccagcgccac acgctgtttc
    58561 tggccgccgg aaagctgcgc cggataacga tccgccagat gcgcaagctg gaccatttcc
    58621 agcaattttg tcactttcgc tttgatggct gcggcattcg ggcgctcgcg acgcggcagc
    58681 accgtcaggc caaaagtgat attgtcgaat accgtcatat ggcggaacag cgcgtaatgc
    58741 tggaacacga aaccgacttt acgatcgcgc gcgtgcaggc gactcacgtc ggtgccgtgg
    58801 aagcgaatat gcccgctggt ttgatgctcc agcccggcga taatccgcag cagcgtggtt
    58861 ttcccggaac cggacggccc cagcaacgcg accatctgac cagaaggaat atccagtgag
    58921 atatcgttca gcacctgggt gcgaccaaaa gacttcttga tattggcaat ctcaatgctc
    58981 atgatgctcc tcctgctgcg cgcgtttttc ctgattctcc aggcgccact gcaacatact
    59041 ctttaaaaac agggtgataa tcgccatcaa cgtcaacagc gcggcggcgg taaaggagcc
    59101 gacggtgttg tagtcctgct ccaacaattc aatctgtaac ggcagcgaaa gtgtttcgcc
    59161 gcgaatcgag ccggaaacca ccgacactgc accaaactcg ccaatcgcgc gggcgttggt
    59221 cagcaccacg ccgtaaagca gcgcccagcg aatattcggc aatgtgacgc gacggaacat
    59281 ctgccagccg gacgcgccaa gcaaaatcgc cgcttcgtct tcctggctgc cctggcttaa
    59341 catcaccggc accagctcgc gcaccacaaa cggacacgtc acaaagatgg tgaccagcac
    59401 cattccgggc caggagaaca taatttgcag attatgttca tccagccagc cgccgagcgg
    59461 gccgttggag ccgtagaaca gcagatacac cagaccggca accaccggcg atacagcaaa
    59521 cggaatgtcc agtagcgtca gcagtaactg gcgtcccggg aagttaaagc gcgtcaccag
    59581 ccaggccagc agaatgccga acaccaggtt taccggtacg gcaatcagcg cgatcatcac
    59641 cgtcagccag atggcgtgca gcatgtccgg atcggccaga ttctgtaaaa ccggcatcag
    59701 ccccttgctg aatgcctgca cgaagatgta aatcatcggc accagcagga tgaacgccga
    59761 aaccagcatc ccgatgccaa tcagaaacca tttgccccag ttaatcgggc gcgcgtcata
    59821 acgcttcaat tgggtaactt ccgccattaa tgacctacca cacgccgacc aaagcgactt
    59881 tgcagagtgt taattgagaa cagcagcagc agagatgccg cgaggatcac cgaagcaatc
    59941 gcgctcgctg ccgggtaatc aaactcctgt aagcgcacaa aaatcatcag cgacgtcact
    60001 tccgtcttcc acgcgatatt tccggcgata aaaatcaccg cgccaaattc accaagacta
    60061 cgggtaaacg acagcgccac gcccgccacc agcgccggag aaagctccgg cagcaccact
    60121 ttgcagaaac tctgccagcg cgttgcacca agcgtttccg ccgcttcttc atattccggg
    60181 cctaactctt ccagcaccgg ctgcacggta cgcaccacaa acggaatgct ggtaaaggcc
    60241 atcgccaccg caataccaag ccaggtatag gtgactttga tatcaaactt cgccagccat
    60301 tcaccgtaaa aaccgtttac ggaaaagagc gaggccagcg ttaaaccggc gactgccgtt
    60361 ggcagcgcaa agggtaaatc catcagcgca tcaagcagcg tgcggcccgg aaagcgataa
    60421 cgtgttaaaa tccacgccat cagcagaccg aaaacgccgt taaaaatcga tgccacaaac
    60481 gccgacagca gcgtcacttt gtaggccgcg accacctgcg ggttggtgat cacctcccag
    60541 tactgcgccc agctcatctc agaaagctgc atcaccagcg cagagagcgg cagcagcaat
    60601 atcaggcaca caaacagcag actggtgcca aggcttaaag taaagcccgg cagcacgcgt
    60661 cttgaagaaa ctgcaaacat tacttacgcc ccgccgctaa cagcttgtct aactcgccgc
    60721 cgctggtgaa gtgggttttc atcacttccg gccatgagcc aaatttgtct tccacgcgga
    60781 acagttcggt ctgcgggaat ttatctttca gtttgtccat gacttccggg ttattcacac
    60841 ggtagtaata gtcggtgatg atggtctgcg cctgtgggct gtagagccag ttcaggtagg
    60901 cttttgcggc tttttccgta ccgttggcct gcacattttt atcgacccac gccaccggga
    60961 attccgccag aatattggtt ttcgggatca ccacttcaaa gccctgtgct tcatactgtt
    61021 tacggatgtt gttcacttcc gattcaaagc taatcagcac atcgcccaag ccacgctcgg
    61081 caaaagtggt ggttgcgcca cgaccgccgg tatcgaacac ttcaacgttt ttcaggaact
    61141 gggtcataaa ctgttcggtt ttggctttat cgccaccgtc agctttatcc gctgcgcccc
    61201 atgccgccag ataggtataa cgcgcgttac ccgacgtttt cgggttcggg aaaatcagct
    61261 tcacgtcgga gcgcaccagg tcgttccagt cgtggatatt cttcgggttg cccttacgca
    61321 ccaggaagcc catggtggag tagaacggcg agctattatt cggcaggcgc gactgccagt
    61381 cagccgggat cagcttgcct ttgtcgtgca ggatttgtac gtcggtcacc tggttataag
    61441 tgacaacgtc tgcttttaag ccctgcaaaa tcgccagcgc ctgttttgat gacccggcat
    61501 gagattgttt tatcgtcagt ttgtcgccgc cgttatcttt tgcccattgc tgttcaaacg
    61561 gcggattcag ggcggcaaac agctcgcggg agacgtcata agaactgttc agcagttccg
    61621 ttgcctgtac atggcccgcc agcagcagag aagcgaccag cgcgagtgag ttctttttca
    61681 gtaagttaac ggccattgcg cacccttata aatttaatga ctttctaata gccatcatat
    61741 ttataacgga cgtgaaagga gtaacggttt tatataccgt ttggtgattt ggaagttaaa
    61801 aagggaataa gaacttggtt caacaggcgt aattgttgca ggcagtttgg acacggacag
    61861 cgcggagaaa ccggagcgta cacatagtac gtgaggattt cgagcactgc ccggggccaa
    61921 aatgacaaat gaaatagcct ggtgaactta gttcaagacg aaatcctccc cacacatgca
    61981 gggaggaaac agaggtgaat cagataccga cgctaaccgt ctccggcagt gtgctgccgc
    62041 catcaatcac attctgtgta ccggttaaat agctggattc atccgatgcg aggaaggccg
    62101 ccagttcgcc gacttccagc ggatcggcga ggcgacacag cgggattgct tttgccattt
    62161 cagtcagcac cgattctgga tcttccgggt tcgactggcg ggcaatgctt tccgccattg
    62221 gcgtgcggac gtatcccggg cagatggcgt taacgcgaat accggattgc gcgtactcca
    62281 ccgccagcga tttagtcagg ccaacaatcg ccgctttcgt taaggcatac gccgtttcgc
    62341 caggatcggc caccatatca ccagtgactg aagacatcat cacaatgcga ccatctttgc
    62401 gcgcaatcat ctccggcagc accgccttcg tgacgttcca tacgccttta atattgatat
    62461 caatatggaa atcgcgatct tcatcgctca tatcgaggaa actgcccaga cgacaaacgc
    62521 ctgcgttatt caccaggata tcaatgcgcc cttctttttc cttcgcgcgt ttgatagctg
    62581 cggctaccga cgccgggtca cgcacatcgg cgacaaccgc cgtacagcga tgaccacgac
    62641 cacacagttc gtccgctagc ttttcgatct caggggagat atccagcaag attaggttcg
    62701 cgccatgacg tgcaaaagtt ctggcaattc cttcgccaat tccctgcaat gcgcccgtaa
    62761 tcagtgctgt cttgcccgtg agtttaccca ttttaatgct ctccttgtta atacagagcg
    62821 ttacactcca ttccctctga aaatacagga taaacaaaat attgctgcgg atcagctcac
    62881 taattcagga aaatctcaat ccagtcgctg ctttatgcat catttcaggc gttgtgttag
    62941 ctcactgctg cgctgaatca ttttcaatga cgccacatca ttgatgttgc acaagaccaa
    63001 caaacagtaa atcggtaaca gaattttgcg ccgtgcgggt agacatcgac gagctacgcc
    63061 attcggtttc accggaaacg gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt
    63121 cagccagaga agtaatggca atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca
    63181 agacaatctc ttttttactg ccgctgtaag agatagcgat ttgtacatcg ccttttttca
    63241 acgcctggga aacggtggct tgtacgtgcg tgtcggcttc gcaggccacg cgatagccaa
    63301 ttttcatcag tttgaacgac agatcgcgcc ccaccagcgc cgatccgccc aggcccgtta
    63361 tctggataaa cggtgcttta ctgatgacct caatgatttt ttgcagccgc gcgtagtcga
    63421 acaacgcaca ggtttgctcc agcgccagct ctttttcgcg attcagtttg cgggcaatca
    63481 cttccagcga atcatcactg gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt
    63541 tctcccggct ggcactgtat tcgccaatta acgccatacg caattcagta aatccttgcg
    63601 cgccgagctt ctgggcaaat ttcacaatgc tcgactggct gataccgagc tgtttcgcca
    63661 tctgccgtga agagacggat ttcagctcgc tcactctggc ccgcagaaag tcagcaattt
    63721 tttgctcgtt cccggtaaat tcactttccg cattgcgtat tttcgtcagg tacaacatct
    63781 tccactctcc tgggctggca gcaaaaattc cgcttaagtt cttatgacgc tcttcacact
    63841 ctgcgagtgt agactcaatg attcctttaa gtctgtcaat tcgggatata tgattccata
    63901 catctgcaat tatttgatcg taaatagtaa ggtcaccacc gatgcaacta gaaaagatga
    63961 ttactgaagg ctcgaacacc gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt
    64021 gccggattat caacgatgaa gataaaaccg taccgcctgc cgttgatcgc gtcctgccgg
    64081 atatcgccgc ggcgatcgat gttatccacg cccaggtcag cggcggcggt cgtctgattt
    64141 atctcggtgc gggaacatcc ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca
    64201 cctacggcgt gaaaccgggt ctggtggttg gtttgattgc tggaggcgaa tatgccattc
    64261 agcacgcggt ggaaggagct gaagatagcc gggaaggtgg tgttaacgat ctgaaaaata
    64321 ttaatttaac ggcacaggat gtggtggttg gcattgctgc cagcggtcgc acgccgtatg
    64381 tgattgccgg actggaatac gcacgccagc tcggctgccg cacagtggga atttcctgta
    64441 atccggggag cgccgtttca accaccgctg agtttgccat tacaccgatt gtaggtgccg
    64501 aagttgttac cggttcttcg cggatgaaag caggtacagc gcagaaactg gtgctcaata
    64561 tgctttccac cgggctgatg attaaatccg gcaaagtgtt cggcaacctg atggtcgatg
    64621 tggtcgccac caacgaaaaa ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg
    64681 gatgtaatgc cgaacaagcg gaagcggcgt taattgcctg cgagcgcaac tgtaaaacgg
    64741 ccattgtgat ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac
    64801 acggcggctt tattcgtcag gttttagaca aggaataacc catggccaaa gagatcagca
    64861 gtgaacttct gaacaccatt cttacccgtg tcggcggacc gggaaatatc gccagttgtg
    64921 gtaactgtat gacgcgcctg cgtctgggtg tacatgacag ttcactggtt gatcccaata
    64981 tcaaaacgct ggaaggcgtg aagggcgtca ttttgaccag cgaccaggtg caggtcgttt
    65041 ttgggcctgg taaagcgcat cgtgccgcaa aagcgatgag cgagctgctg ggcgatgcac
    65101 cggtacagga tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa
    65161 cctccggcgt gcaacaattt ctcgccaaat tcgccaccat cttcacgccg ctgatccccg
    65221 gttttattgc cgccggtctg ctgctgggga tagcgacgtt aattgccacg gtgatgcacg
    65281 ttccggcaga tgctcagggc acgctacccg acgcgctgaa ttttatgaag gtgttcagca
    65341 aaggtttgtt caccttccta gtgattctgg tgggctataa cgccgcccag gcatttggcg
    65401 gcacgggcgt caatggcgca attattgccg cactcttttt gctcggttat aaccccgctg
    65461 caaccaccgg ttactacgcc ggttttcacg atttctttgg tctgcccatc gatccgcgcg
    65521 gcaatattat cggcgtgctg attgccgcgt gggcctgcgc ccgcattgaa ggcatggtgc
    65581 gccgctttat gccggacgat ctcgacatgc tgctgacctc gttaatcacc ctgctgatca
    65641 ccgctacgct tgcgtacctg atcattatgc cgctgggtgg ctggttattc gaaggtatgt
    65701 catggctgtt tatgcacctg aacagtaatc cgttcggttg tgcggtttta gccgggctgt
    65761 tcctgatcgc cgtggtattt ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa
    65821 tggacagcca gggattcaac agcttatttc ccatcctttc aatggcaggc gcgggccagg
    65881 tgggcgcggc gctggcactc tactggcggg cgcaaccgca cagtgcgcta cgcagtcagg
    65941 tacgcggggc gattattccc ggcctgctgg gcgttggtga accgctgatt tacggtgtca
    66001 ccctgccccg catgaagccg tttgttaccg cctgtttagg cggcgcggcg ggcggtttgt
    66061 ttatcggatt gatagcctgg tggggtctgc cgatgggctt aaacagcgcc tttgggccgt
    66121 ctggtctggt ggcgctgccg ctgatgactt ccgcacaagg catccttccg gcaatggcgg
    66181 tttatgctgg cgggattctg gtggcatggg tttgcgggtt tattttcacc acgctctttg
    66241 gctgccgtaa cgtcaatctg gactgatatt atgaaacgga caatgctcta tctttctctg
    66301 ctggctgtta gttgtagcgt cagcgccgca aaataccctg ttctgacaga aggtagttga
    66361 tcaagtatat gcggcgttaa agcagtaata aaaaggccag tcagtaatgt tactgcctgg
    66421 cctttaatca atgaatcaga aacgcttaca gcgccatcaa cttgtccagc gacggtgcga
    66481 aataatagcc gccggttacc ggtttggtga aacgcaacat cgcatcacgt ttgccatcgg
    66541 tatcgccaaa catgctcagc agttgctgct caatgttatg cagacgcgcg cagtaggcgc
    66601 agaagtacag accgtgagtg ccactggcgg tgccgtacgg caggctctgg cgaacaatct
    66661 tcagcccttt gccatcttct ttcagatcaa cgcgggtgag gtgagaggtt tccggacgtt
    66721 cgtcgccgtc aatctcttcg ttggcctctt tggtgcgccc gatcatcatc tcctgatcgt
    66781 gaacgctcat ccggttgagc tgcttcaggt tgtgctccca acgctgcaca aacacatagc
    66841 tgccgccgga atccacgccg tctttgataa ccgccacttc gcggcgcgtc tcttcacccg
    66901 ccgggttttc tgtaccgtca acaaagccgc tcaggtcacg ctcttccacc caacggaagc
    66961 cgtggatctc ttctttcact tcaatgcagt caccaaaggc ttccatcgcc gcctgagcga
    67021 cagagaagtt tacgtcgtga cgcagagaaa gaatgtggat caacacatcg aactgggtcg
    67081 tcggcgcaag gcctttaccg tagcccggaa aatctttcag ctcttctgcc ccaacgccgc
    67141 cgctcagagc gcgccaggtg ttgttaccaa aggcaaccac cgcaccaaga tgcgcgtccg
    67201 ggaatttcgc ttcaaaagtt gccagtttgt cggcaaatgt tttactggcc gcacgcaggg
    67261 cgtcaacttc ccctttcacg ttggcttcga tccaaatcgc cgcgcggcaa tgttctggca
    67321 aaatgccact ctgaacctga gacattgttc ctcctgaaaa taagaatgcc acgcaatccg
    67381 tggcattgtt gagcgctatt ttacgtgttt tttcgcgacg gcggtttgtt cagacgcaaa
    67441 ttaacgccgc cagataattt tactgacttt ccagtttttc agggtatcgt ccgaaggcat
    67501 taacccttca ggaccacgcc attccccgct gaactggtag ctaatatgtt gactgccttc
    67561 ggctttacat tcgacggcac gattatcatc accatcagct ttttggcaat tgccaaatgc
    67621 tttgctgtaa aggtcgctaa acggtgtacc gattttaaca ccggtgtcag caggaatatc
    67681 gctatccagc acgtcaatgc gactgatcgt gccttgatcg ccgttaatca ccatcgccac
    67741 gttgtcgcct ttcatcactt caaaaaagcg caccacgttg ccgttcgtgg ttttcattcc
    67801 gctgcgcagg cgataatcgc catcaagcgc atcagcaatg gcttgttctt gcagtggtgt
    67861 ggacgccgtt aattcaccca cgccctgctc gctcactttg gtggacgatc caaaccagtt
    67921 ccacgggtta gcggcagacc agttaactga cgacatcgtg gaacagccgg tcagcatcaa
    67981 cggcatagcg cataacatta aacgcagcga tttcatgtca cttcctttgg ttattcaata
    68041 acgttgcttg gagtgcaaat tcaccaaaaa gtgccgttat tctttttctt cattaaaaca
    68101 agcgcgtaaa cgtcgattgg tcagcagcca gattaacgcc acaatatccg ccactaccag
    68161 cgccagaccg ataccgctaa cggattcacc gttcagccac agccacggtt gccagcaaag
    68221 taaaaccacc tgcgccaaca gcaacagaaa atagagcaca tgccaggtgc gaggaaacga
    68281 agcccgccga ccgctcaaca aaaacgccag caccgccgga ataccgggaa tcagccccag
    68341 ccagaaatta tcgtgatcgg gataaaacag atttagcagc gcagtaccct gctcacgcga
    68401 cgcaccggca atgacaaaca gcacccaggt tcgcgcctga agcaatagca caagccagaa
    68461 gagcaagggt aaacgcaggc gtccgtgcgc atcataatgg acaggatgga actcagtact
    68521 cttcatcttc aatcaaacgc ttacccagac tcagcacgtc ggcgtgttca tatcccaggc
    68581 gttcatacat tccgagcacc atgtcgttat cttccggcac gttgatctga attttcgggc
    68641 agccgcgagc aatcagcttt ttctccagcc ggttaagcaa cgcattggca attccacgcc
    68701 cacgaaactc tggatgcacg ccaagataat aagcagaccc gcgatgcccg tcataaccgc
    68761 ccatcaccgt tccgaccact tcaccgttta cctcagcgac caaaaacaaa ctgacgtcat
    68821 ggttcatctt acgctcgatg tccatttccg gatcgttcca cggacgcagc aagtcgcaac
    68881 gctcccaaag ggtgatgacc tcttcgaaat cttcctggcg aaatacgcgt atctccatgg
    68941 tattcgttac ctttttgcgg gttaaaaggc tgattatggc gtgaacggtc gaattagcca
    69001 atatctgacg aaaatcggtt gaaaaagtgg cataatgggg agttgtcaac tattgaaatg
    69061 aaaagtaaaa caattctcaa cagcaaaccg tcgtaacgga ttacgcgata cgatataaca
    69121 tctggaactt tattattaca actcaggccg tatgagcact tttaaaccac taaaaacact
    69181 cacttcgcgc cgccaggtgc tgaaagccgg tttagctgcc ctgacgttgt caggaatgtc
    69241 gcaagccatt gccaaagacg aacctttaaa aaccagcaac ggacacagca agccgaaagc
    69301 caaaaaatct ggcggcaaac gtgtcgttgt tctcgatcca ggccacggcg gaattgatac
    69361 cggagcgatc ggacgcaacg gttcgaaaga aaaacatgtg gtgctggcga ttgctaaaaa
    69421 cgtccgttcc attttgcgta atcatgggat tgatgcgcgt ttaacgcgtt ctggcgatac
    69481 gtttatccca ctttacgatc gcgttgaaat cgcccataaa catggcgcag atctgtttat
    69541 gtcaattcat gccgatggct ttaccaaccc gaaagctgcc ggtgcttcgg tatttgccct
    69601 ctctaaccgt ggggcaagta gcgcaatggc gaaatacctg tctgaacgcg aaaaccgcgc
    69661 cgatgaagtt gccggtaaaa aggcgactga caaggatcac ctattgcagc aagtactgtt
    69721 tgatctggtg caaaccgata ctattaaaaa cagcctgacg cttggctcgc atattctgaa
    69781 aaagattaag ccggtgcata aactgcacag ccgcaacacc gaacaagccg cgtttgtggt
    69841 gttgaaatcg ccgtcggttc cttcggtgct ggtggaaacc tcgtttatca ccaacccgga
    69901 agaagagcgg ctgttaggca cggcggcgtt tcgtcagaaa atcgccacag cgattgctga
    69961 aggcgtgatc agttatttcc actggttcga caaccagaaa gcacattcga aaaagcgata
    70021 agttatgaaa cccgacgcac accaggttaa acagtttctg ctcaaccttc aggatacgat
    70081 ttgtcagcag ctgaccgccg tcgatggcgc agaatttgtc gaagatagtt ggcagcgcga
    70141 agctggcggc ggcgggcgca gtcgggtgtt gcgtaatggt ggtgttttcg aacaggcggg
    70201 cgtcaacttt tctcacgtcc acggtgaggc gatgcctgct tccgccaccg ctcatcgccc
    70261 ggaacttgcc gggcgcagtt tcgaggcgat gggcgtttca ctggtagtgc atccgcataa
    70321 cccgtatgtt cccaccagcc acgcgaatgt gcgttttttt attgccgaaa aaccgggtgc
    70381 agagcccgtt tggtggtttg gcggcggctt cgatttaacc cctttctatg gttttgaaga
    70441 agacgccatt cactggcacc gcaccgcccg tgacctgtgc cagccatttg gtgaagacgt
    70501 ttatccccgt tacaaaaagt ggtgcgacga ttacttctac ctcaaacatc gcaacgaaca
    70561 gcgcggcatt ggcgggctgt tctttgatga tctgaacacg ccagatttcg accactgttt
    70621 tgcctttatg caggcggtag gcaaaggcta caccgacgct tatttaccaa ttgtagagcg
    70681 acgtaaagcg atggcctacg gcgagcgcga gcgcaatttt cagctctacc gtcgcggtcg
    70741 ttatgtcgag ttcaatctgg tctgggatcg cggcacgctg tttggcctgc aaactggcgg
    70801 gcgcaccgag tcgatcctga tgtcaatgcc gccactggta cgctgggagt atgattatca
    70861 gccaaaagat ggcagcccag aagcggcgtt aagtgagttt attaaggtca gggattgggt
    70921 gtaactccct cacccccatt cccgcatccg ctgatgcaac gtcaacgacg gcttctcggc
    70981 aaacagctgc tggtaatccg tggcaaattg ccccagatgc cagaatcccc actgcatggc
    71041 ggcgtctttt accgttgtgc tttgcgacca cgggcttatc agttcgcggc gtacgacgtt
    71101 caggcgaatg cgtttcagcc acgcgtttgg gccaatgcct aaaatagcgt gaaacgcgtt
    71161 ttgtagcgtg cggcgactga catgcagttg attacacaaa tccagcaccg tcaccggttc
    71221 ggacatgttt tccagcacat attcacgggc gcgggaaagc aaccgacggt aactctgatg
    71281 actgatgctt tccgccgtca ccattggttg cgcttcttcc agcatcgccc ccatcgccat
    71341 tagcaaatta tcccccagca cttttcgcac tgcaggctga tggagatttt ccgggttctc
    71401 gcaaaacgtc gccagcgcct gttggacaaa gccccacagc gcggctttat gctgctcttt
    71461 cacttccagc gccgactggc tacgcagcat atgtaatacc cgatccggat tgtgcaaaaa
    71521 gttagcctga caggtgatga catcttcaga cagcactacg cccaggatcg tataatcgtc
    71581 cggcgtactt aattcaaact cggttccacc ggggcgcgtc gctatttctg cgcttcccag
    71641 acattgcgaa ccgataaatc cctgctcacc gcgcgtcgcc ggaatgccaa accagaacga
    71701 gttcggccag accaggcacg actgacgcag cgccagaccg gtgtattcac gaaaaacctg
    71761 aatatcatcg agtagaattt ccgtaaattc accatgaaac ttgcccggat gcagctgatc
    71821 gtaaatctgc tgctaggcgg taatcgtcaa agcatgttca tagacatccg ttgtctgtcg
    71881 ttgatgaaca ttatccactt cgaccttcgg cgtgagcttc aggttttcgg gtaagggttc
    71941 atgataaaga tggtgcaaat tggctgtacg ggtctttttc atgatgttaa tgccgggtgt
    72001 tgtaggacac ccggcacctc cgacaggtta atggggcttg agacgataac gactactgcg
    72061 tttacgtaac gtcccggcag aaaagagctg ttcgagcgca tttctggctt tttccagcgg
    72121 ccagccaaag tgggcggcca cttctcctgc cgtcattccc tgacatactg atgttaacag
    72181 cgccaacaac tcgtcagcag attccgcgac gataactggc gcgtcgggtt cctttacggg
    72241 ttgcttcggc tggcggttaa agccagtaac cagccactgg atgtcatcgt ctggccgacc
    72301 gatctcctta cggctgatga cacgcccggt acgcatcgcg gcagcacaac ctgcgtccag
    72361 cgccgcacga cacgccgcca gatcgccttc caccaccagc gttaagcgac cagggtcaag
    72421 cacttcgtga ctgagcagac gaacgttagc tgctttgagc atggcatccg ctgcatctat
    72481 cgcggcgacc atgccgtcca cttccagcaa tcccagtgca ttgatcatcg gcaacctccg
    72541 ttacgcacgc tggattggat tacgcgcgat ttccagcact gcatcggtaa aggcgttaca
    72601 ggctgcttta cacgcggcct ggctaccggt taaaaatgct gccgagtagt tggtttcaga
    72661 cggtggcggg acgtaggtca ccagctgtac gtcggcagat ttcaacgcgg catcaatgcc
    72721 gtaggtcgct tccagcggcg gtgccaccag atacgccatc ggatcgccaa gcgtgatccc
    72781 ggcggttgat gaaagataag aaccggtacg cgaaaccaca tgcgccagga aggcggtgtt
    72841 ttctgcgtca ttggcccact ggaaagctgc gccattttca atatgtgcaa ccatcgcatc
    72901 cagaccggca cgcacttccg ccgggtttgg cccaccaagc ataatcagca cttcaccggc
    72961 agtcggtgac gggccgtgtg ccgctcccgc atacagcgag cgaccatata ccacttcgac
    73021 catcgcttgt ttggtcgctt cgtcagccgc aatgtaggtt acgtcgtcag aatcggcaga
    73081 aatcagcccg aggctacgaa tatgcggcgg caattttagt tcacgcgcaa attcagcgtt
    73141 aacggaggca atcacccgca tggcggttac cgacggtcga atcaaatcta aagctggcat
    73201 gatgcctcct tatcgggtca tgttgatgcc ggatgctttc tgctccagca tacgtttcgc
    73261 caaatccaca atgacggcac agcagcacga aagagaagca aaagtcagct acctggtaca
    73321 ctaggcagcg ggtcagcaaa ggggggcaaa tgcccctttt ttcatgtcag ataatcagat
    73381 cattacctca ataaactaat aatcacatcg cggttacaac cagtttgata gtgatagctc
    73441 aaacccagac gtggcgctgg ctgcaagcaa aataagccgc aaaaatgcga ccccatgttt
    73501 actagccgca aaaatgcggt cctatttgcc agaacctttt attattcaat tggttagtgc
    73561 aagaaaaagc gtgattgctg ttcaagaagt atccagaaag gtcatgattt tgacgctgaa
    73621 aaaaaatagg tgggcatttt tgcccactca ctagccgcaa atttgcccac tcactagccg
    73681 catttttgcg gcccaaagta ataaatacag agaaataata aatacagatc agcgccaaga
    73741 tttttcgtct tttcggcgga atcatgagac tagtgcttac gtcctgataa cctccagagc
    73801 agaccttaac ttctgtgcag caggactacg ctcattgccg ctcggctagg tcaaatcctc
    73861 acgataaagc tgttcggatt gtgaccataa tgttaaagga ctctttaaat cattccttga
    73921 cgaattaact agctggctta agcggaatag catgcaggat aaaaacaaaa ccagcttagc
    73981 agtggggaga gcttcgcttc gctcgtctcc tggggccaat gctcaatgaa acgacgtaag
    74041 gcacggagcg ctcaccatag gtagacacat aaacatctga gagcgcacct tctatcgcgc
    74101 tacgcgctca ataaaaacca acgacaccgc cacactgcaa gaccaatggt ttttatcgcg
    74161 tatacagacc agacaggaaa cagcagcaaa gcagcgtata gccaggtgca gacagcacac
    74221 tcacacgacg cacagcaggc ttaagtgatc actcaggaaa ctttatcccc gccgccgatc
    74281 accgcgaact atcatgaccg cgccaaattc gaaaggaatg gcccatagaa cgcgatgaac
    74341 acacataagc gctacactta cttaacgtcg ccacagagcg attgagagcg cttaaaatcg
    74401 atgcgatttc attaaatttt tctcactcgc ctgaatggtg gcgatcagga ctgatcgcaa
    74461 cattgccccg cctcgatatt gagcgcataa tagatacata cattaagcgg aaaagagata
    74521 agtttattgt gaaaatcagg gggttacata tcatgcgcat atcagaacaa caacggcaga
    74581 tcctcgacta tctggcaaaa aacgaatcaa tcaccgcacg acaagccgca gatctcgttt
    74641 atggtggcaa tgttacccgc gtccaggttg acgccgcccg ccgctcactc atgacgatgg
    74701 ttagtaatgg gctaatcagg aaacaaggcc gcgctttcgt agctgaaaga catatcaatc
    74761 acgcttatag ccaggacgtt gaagaattgc gccgccagat cctttgggta ttcagcaaac
    74821 agggtcagga gtatttcctt tctgaacacc caaatttcgc cgccaaagag ttaacagtaa
    74881 gcggattgtc gtgcgcactg gattatcttg tcggtgaagg ttgcgttact tacaagcagc
    74941 tatggcacgc cctgaatacg cttgtagaag atggtttact cattgccacg cgtgagaaca
    75001 tcgaatactg gataatgccg cgttcaggtc gccgcaatca tgcacggtat atttcaggca
    75061 acagatacag aacgccggac tttaatccga cacaggaaga tcgggaccgt gccgaaatga
    75121 tgtgcaggga acaggagaaa agaattatgg ataagttatt ttcgatgcag tgaacacaag
    75181 cgggcgcaat gcccgttttt attgccttat tccacgcctg acgaatcaat ttgatcacac
    75241 ctgagaaaat gcatagccag aagttaaggc cgttatattg cgattgggag cgttttaaac
    75301 ggtatcaatt taattaattc tgatgtggtg atttgcaggc aaaaaaaacc cgccggagaa
    75361 gcgggaaata gagacatatc gttgtagtta ttaacagatc actttgaggt cgagggcggc
    75421 taaccctcgc taatattatc ccgctattac cttgcgggtg caaataatct tttattgctt
    75481 tgatgattta ataaatagca ctttttgcta acaaaaaccg ctgtcaatat atggccaaaa
    75541 atgtcaacag aaaaaataag gtttataata ttaagtatca catgggtaat gggttcaggg
    75601 aatcggaatc caccgcctca cggaaattca aaatttgctt cttctttcct ccgtgggcgg
    75661 cttctttttc gtatcgaggt atttatgaaa gcaataacat tagaacagtt cgcagcaatg
    75721 gcgaatgtat cgaccagaac gctgaggcgc tggctaaaaa aaccatcaac cggagtccct
    75781 ttgccattta ccccacgcgg tactcgccaa ttattctggg agcacgaatg caaagaatgg
    75841 ttgtacagaa cagaacgata ataatcacgc atcacatttt gaggtaaaca tcatgaccaa
    75901 tatcgaaaga ttagaacgct gtgccgatat tatgcgacgc agatggattt atgaccctga
    75961 agcgggttta ttgtttagcc gtgaaacaaa agacgttatc aaaggcagca tgaccaataa
    76021 agggcatttg gttgtaacgg tacatgaagg taaattcaaa gtgacactca cctatcagaa
    76081 agcctgctat gtatacacat acggcgctta tgacgagaca ttatatgaag tgcaccacgt
    76141 taatttgaac aaacaggata accgcatcaa gaatattcgc ctgatgccga aagaaaagca
    76201 ccgccggata cataaaaacg tccgcaaact tattcggctg ggccacacac aatggcttca
    76261 accgcaatta attgaacaat cactataaac acaggagtaa ataccatgca ttcattagac
    76321 agttattttc agcgcacgac agcccctaaa tcagccgccc aggaacgccg tgaggaattc
    76381 catgagaaag tgatgcgttc tgctgattat attgcggaca agttcgtgga aacggttcgt
    76441 ccacttgttg atgaagtagc tgataaactt cagtctgaaa tgccggaaga tatggaaggc
    76501 actgcaaagc ggcgactcat ttgcgaatta agtcgtcgtt ttggcgtgtc aataagcgct
    76561 tttaaataat cgaaaaaagg ggggaaagat aatggacgca tttgaggtac tggcaaataa
    76621 aggcgctgaa ctggtagcac agcgtgataa aaccgccaac gaaggggaac gcgcactgct
    76681 aaataaacag attaaagcga tccgtatggc ccagtttaaa ttaatcagta atgaagttat
    76741 cgaaacaatt aaaccgcaga tcccacaaat cgttgctgac gctattaaag ccgccggact
    76801 ggagaaacgc attgccgggc taaaaacgaa cgcagagaaa atggagatgt acaaacagag
    76861 cggtgctccg aatcccgatg agtacttctc accggatgtt gagtttatgg cgcaggttga
    76921 ggaacgtctg ggtagctgtc tcaccgagga acagcgtcgc tattttgatg gagtggatag
    76981 ttcggcaggt attgacctga actcgtattt cggtcgtgaa attgagcatt ttgatgccgc
    77041 cccgtctatg ccggagccag aaccagaaat ggccaccgat cctgaagctg agcaacggaa
    77101 acgcgtattc aatgcgatag ggattcggta ctgatcggat aaaagaaagc cgggaattac
    77161 cccggctgac ttaacaatgt gaccaatgaa caaatcggac aagacaaaca ggataagcag
    77221 tctcgaaaca atgttcggga actatattag cacaagtccg gagaaaatca tcatgttggc
    77281 actacgcatc atgcagggca tcgctaagac cctcgcagaa catgtcttag acctgaaaca
    77341 ttcgccactg agcaaacagg ccatgaagcg ccaaacgctt cgtctgtggg ctgaatattc
    77401 gcttggaact atcaataaaa tcatcgacat gaaatcgggg ccgagcaacc agagcgcgga
    77461 ggaaatggag ttcatcaggc gtttaattct cattcgcaga gacattcata gccagttaca
    77521 cagcgtggga atcgatatta atgatgggac gggcgattaa ttttcggaca agtactatgc
    77581 gcgcgcgagg agctgaacat gaagcaagat attgcagaca ggctggagat actcgaaggc
    77641 cagcgggcag aagcgaaaca actacgtaag caggccagaa gagcgcacag gaacaacgaa
    77701 gcagagttac taaccaaata catcagtttc accaattact gcatctacga gtgttacaag
    77761 gaagatgcag aggactggct ggattcattg ccagaacagt actgagcaga ataacaccac
    77821 gtcatgttgt tctgaggtga gcatatcacc tctgttcagg tggccaaatt tagtgtgcca
    77881 cttcagcatc accgttgaaa tcacaataac gatttttttg taaatatgct ctcacatatg
    77941 taggaataga ccaggttggc attgaaacac atctaccatg accataataa agtaattttt
    78001 tgatatgttt atggttttat attaattaaa atatatctaa atttgatagt tttaaaattg
    78061 caaaatccag attgtgtttg taatgtttaa tctgattgta ttgagtggcg tttattatga
    78121 aaaataaatc aggtaattct tgttatagag aagcagtcga tattcatagc tcacttgagc
    78181 tttcatttat attgcataaa tatcatttgc gtgtagatga gaagaaatct cgaaatgtat
    78241 tcagcgccat acgtgatgag ctcatccttg atggggattc acgacaaaac ttagctactt
    78301 tctgtcaaac atggcttgat gatgaaatcc gtgagttgct taatttagct attgataaaa
    78361 acatgattta taaagatgaa tatccacaaa tggcggaacc tgaaggtcgc tgtgtgcgta
    78421 tgctttccga ttcgtggagt ttccccgatt cgcgacacac cttaggttgc tcaaccattg
    78481 gttcatctga agctgctatg ctcggccgct tggcgttaaa atggcagtgg tgtaaaaaac
    78541 gcgaagtaca aggtaaatca accgaacctg atttgcgatc ctgtgcaaat atgctggcat
    78601 aaattctgcc gttactgtga tgtgaaattg tgtgaaattc cacttgatga tcatcacctg
    78661 ataatgaatg ctaaagaggt tttgaagcat gtcgatgaaa acactattgg tgtcgttacg
    78721 actttaggta ttacttattt ttgtaaagca gattgcgtta gtgtttttaa gataagtgtt
    78781 aaagttaata ttagttttta tatttacatc gtaattatat gtttctattt taaaaatgtt
    78841 cgagagtata aaaatgtcta atgctaatat cgcacccaaa agtaagaaaa tgacagtggg
    78901 gacactcgct atcatgaata taactgctgt agtaagtcta cgatttatac cttctgaagc
    78961 agaatacggc ttaggtgcca ttttttacta tacctttgct gcaattatgt ttttagtacc
    79021 tgttgccttt gttgcggctg agctagctac aacatatcca caaaaagggg gggcatttcg
    79081 atggatagga gaagcatttg gggctcgctg gggttttgta ggaatgctta tgacctggct
    79141 acaagtcatc ccctactttc caacagtact gacatttggt gcagtctcgg ttgcatttat
    79201 tgatgttgat atgggggtgg cagaatcaat tgccagtaat aaatggtata ttttattttt
    79261 cgtactattt gtttactggg gagctatttg tgttgcatta cgtggcgttg gtattttctc
    79321 gcaggtatct aagtggtgcg gtattatagg gacaattatt ccagctatta tccttgttat
    79381 tcttggtttc tcctatctgt ggttcagcgg taaaccagcg gtgatcccat taggttgggg
    79441 tgatcttttc cctaatatag ctaacttcca gaatattgtt cttgccgcca gcatatttct
    79501 tgcttatgca gggatggaaa tgaatgcggt ccatgttaac gatttagata atccaactaa
    79561 aaaataccca attgcaatta ccatcgcttc tataggtact attgcaatat tcctgctcag
    79621 tactttaggg gtcgccttta ttattcctac agataaaatt agtctaactc aaagtttgtt
    79681 ggttgcctac gatatgctgt tcgaatgggc tggagtgcca tggcttggct ctgtgatggc
    79741 atttatgcta gcaattggcg tactgggtgg ggtggttact tggattgcag ggcctaatac
    79801 aggtgtatta gcgatcgcga aagctggtta tttacctaag tttttccaaa aaaccaatcg
    79861 tcatggaatg gggcaccatc taatgtttgt acaaggtata attgtctcag ttctttctgt
    79921 tacttttgtg ataatgcctt cagttcaagc agcttttcag attttatcac agttaacagt
    79981 tatgctctat ttagttatgt atatgctcat gttcgctagt gctatctatc tacgacattc
    80041 tcaacctgat acaaaacgcc catatcgagc ccctgcttta tttttctggg caacacttgg
    80101 attcttaggg tcttttctgg cttttgtatt aagttttatt ccgcccgagc aaatcccaac
    80161 tggtagtcca accagctatg ttctttattt gttagtatta gttgccctat ttttattaat
    80221 tccaatatta atttacgcct tccgaaaatc tgactggaaa gatccaaact ctgatttcga
    80281 accattttct tgggaaaaaa gaactagtaa ttaaattttc cataagctac aaaagatgag
    80341 gttaaatatg tttagtgctg aatctccaat tggtgtaata gactcaggtg tcggtggatt
    80401 gacatcggta aaggaaataa ttaatttgct tcctggtgaa gatattatct attgtggaga
    80461 taatatgaat gcaccttatg gcaaccgttc tgccgatgat atcatcagtt tgactaaaaa
    80521 aatgctgact tttttacagt caaggaatgt caaactagtt gctgtcgcat gtaatactat
    80581 atcctcaact ttggaatccg aagaatacag cggatatgct aagagctttc cgtttcctat
    80641 cttatccatc attgaacctg cagtagaaga tgtgattcgc cagcaatata aaaatgttgg
    80701 tattatcgct accgagttta ctattaaaac ggggtgtcac aaagagctaa taaaaaaatt
    80761 gaattcaaca ataaatgtat ttggagaacc aagcaaaaat cttgcaatgt taattgagga
    80821 aggtaatctt aatgctcctg caatcttgaa tgacatcaaa aaacatgtta accatttaat
    80881 ttccctccat ccagtaaatg aaattattct cggctgtact cactatccaa ttgttcagaa
    80941 tttttttgaa gcagttgcac cggatataaa atttattaat cctgcacatg atcaggctat
    81001 ctctattaaa aatcacttgg gacagctaaa tttattaaac tcctcaaata taggaacttt
    81061 gcaaataaat actagtggaa gtatggagat ctacaaaacg gtactagggg aattgagcat
    81121 tacgaaacct catacattca gtatcaggca gttttaaatt tatccggtac accgtaaaat
    81181 cccgtccttc tgaactgcac cccaaaagtt ggacaccaac tgagtaaggt gcattttatg
    81241 gacaaaccca gttacatact gggtagtgca tccaattagt agaacatgtg tttttcgata
    81301 aacgctccga tcactttttc gtggatctca actgagcgtg agaagcagat tgttttacga
    81361 cccaaccgct taatgcgggt gcgtagcgtc agattattac gctcaatgcg ttgggtgaat
    81421 attttgccgg tcagatgctt attcttcggc acctcccggc catagctgcc ccagtcatcg
    81481 ctggtcagca tgccgatgtt gaagggtgta agcagtgcca gtagctcccg gcacgtttga
    81541 tcggttcggg gaccaaaagt gtaggccagt acaccgcctg ttttggtgtt gtacgcgtac
    81601 ccgagccaat gttgccgggc tttactgcca acgtagctcc attgctcatc aagctcgcag
    81661 ataaacgcca catcagcatg ggcaacaggc gaagaagtta ttcgctttgg cgcgagtttt
    81721 ttaaagtccg gatgacggtg ttactaccaa ttttcagtgt cctggcggta tcgcgaaccc
    81781 cggcaccatt aaaggccatt tcagttatca gctctttcat acccggctta cgtgcttgat
    81841 aagtataagt gaactgaaac acacggtggc agtcacggca gcgaagtctg tcacggcctt
    81901 tagggttctg accatggcgg taaacctgag ctgactgaca acggggacaa tgaatattaa
    81961 cgctggtcat gagataacct caaaagcccg tattatacat cagattcaac taattagagg
    82021 catcacccat ccagcattat aaaacatact aacaataact aaattataat aatcacaact
    82081 tacaatcaaa taataaatgc ataagattaa caatgatttt ttcgggttat atttatctaa
    82141 aaaagcagaa tattatgtaa cttattattt tctaagcgaa aatttattgt gcggtcagac
    82201 taacaaactt gcgtaccacc cgaagaacat gcgaagattt aagtattctt tcttttttgg
    82261 tttttatttt tcattttctc ttttcttctc tttccggttc tgttctggtt ttggtttttt
    82321 cctttttatt tttattcttt cttcagggtt atgctaaaac accaaaacat ttttcaccac
    82381 aaaagcatcc aatcatcttg ccagaccaat caccttaacc ggatacactg ccggatataa
    82441 tcccactaac acccagacac caagcaccat acctgataac taccgtattc aggaaggaaa
    82501 tgcgagtgat ttcaccgtca ccggcacaac cgatccgcca aaaagaggcg taccaatgtc
    82561 gatatagtcc cccgcgcgga caatcacttc gtcaatgact gccaacggga gttgttgtag
    82621 ctgtgggcgc aacaacatgc ccagagcttt accaaagtcc tgcccggcca ccaccagcag
    82681 gggatgcgga ttcggaaaac gcgcgacgaa atcgaccagc gcgttgatga ccgtcagaac
    82741 cgcggcgtaa cgcacaggca gcgaggcggg aagcgccagc acgtacacgt cagttttggg
    82801 atcaagatcc agctgaagca gcgcctgttg ccaggcactc accagatccg tttcatcaat
    82861 cgggatcgcc accggcaaat tgcgcagcgg cagttgtacg ccctccagcc agattgtgct
    82921 gccagagagc gaaagggtat gtgcacccgc gccaatcacc gtggagcgta cggtttgcgc
    82981 cggaaactgc acattcatct cacgcaggcg cggatggtca tgcagcgccg ttgccagaag
    83041 tgggccaata tcggcaaaac agaacgggtt ggcgggctgg tggcgataac attcgcccac
    83101 gcctccagaa agcgtaatga tttcgggcgt aacacctgcg ggcagcaaac cggtttgcat
    83161 caatgcctgc gcgagcggcg aaagcgttcc gtcaatcact tcgacaatca gcgccgccat
    83221 ccgccgggta acctgcacca gctgcgcgcc ggtcagcgaa cgggcgtcag tgcctgcacc
    83281 gaagcactca tccacaatca tctgccccgg tttatgagcg taaaccacgc gcccctggct
    83341 gtcggtttcc agcaggcgac caccgacgtt gagacaggca gtgccgctga tttttccggc
    83401 atcgaacagg gcgtagttcg cggtgccacc gccgatatcg atattcagta cccgacacag
    83461 ccgttgttca gaaagggttt gcgccccggc tccgtgaccg gcgatcacgg attcgaggtg
    83521 cggcccggcg ctggcgacga caaaatcgcc cagcgactgt ttttccttca tcactttttc
    83581 catcagctgt gcgaccaggc tttcggtaaa ctggccttcc ggcagctggg caatgatggt
    83641 ttcgcggatg cgctggcttt cggttttgtc ggctggcacg ctggcgggca cggacgccgt
    83701 taccgggatc gattcatcac attcggtgat ggtaaaaccc agtagatccg ccacttcacg
    83761 ggcttccggg gtaataatgc tggcgcgcag aaccacggac attgcctgtt cgccgcgcgc
    83821 gtgtgcttca cgaatatcat tcgctgtgat gagttttttc acctgcttcc tcctgtttgg
    83881 tgagagatgc cagataatcc accagttgct gcacgctttg cgggtcgtgg ctgtttagct
    83941 cgaaaatcgg ctcttcgaaa cccgtctcaa gcaataactt tcgcgttgcg gcgacgtcag
    84001 catctggcat gtccgtttta ctgatgacgg cgatttgccg tttgctgacg ccaatatcca
    84061 gcaacccggc aggcaaccga ctttccgggt catttgcgcc atggacatag atcagcatgt
    84121 ctacatcctg tagcgtggta attaaggcgt gataccagcg gggatggcta aaatattcac
    84181 ccggcgtatc aatatcgccc ttatcattaa attccacggc cagtgttttt ctggcgaggg
    84241 tataattccc ctgcaacgca ttaaataacg ttgtttttcc tgccccgacg ctacccacaa
    84301 acgcaattcg tttcatgaat gcctcgtatt aacttttggt catttcacaa agcgtataat
    84361 ttaataagcg acctaaaccg ctgacggttt gtgataacgc ttcttctact gcgcccaccg
    84421 agccataaat caccaatgca ccgctgaagc ggtcgagaaa accgatatgc acatcagcgg
    84481 ctttcagcgc taaatcaccg gcgatcatcg ccgtttcgcc gggagttagc gtcataatgc
    84541 caattgcgcc cgcatccgga acgccgattt tcttcgccag ttcctcaccg ggatgcgcga
    84601 tgagatgcgc cagtgtgacc tgtttgccgg gcacaaattc ctgaatgatg cgttctttat
    84661 ccatgagtcg ccgcctgtga aaaactttcg tgtatcgtga caatttttcg tcagagtaaa
    84721 taacaaaatt cggcaagcgc gtttaaaagg tgaggtagat caataagaat ggagagatat
    84781 ttactgttat gaataaactt tcatatgcga cgcatgaata taaataaaat aatatcgaga
    84841 tattttatat actgattttt ttattattcg caaactcagt aataagcttt gctaatatat
    84901 ttcaggaaaa ccgcttaccc tgaatattca gggtaagcgt gagagttaag aatgaattac
    84961 agcggttggg tttgcgcttc aaccacggcc agcgccacca tattgacgat acgacgcacc
    85021 gatgcgatcg gcgttaacac gtgaaccggt ttcgccacgc ccatcagcac cggaccgaca
    85081 gtcacacctt ccgagctgga aacgcgcagt aagttgtaac taatgcgggc agcttccatg
    85141 ttcggcatca ccagaatatt ggcagaacct ttcaaagggc tgtccggcat acggtcgttg
    85201 cgaatcgctt ccaccagcgc tgcatcgccg tgcatttcac catcaatcat cagatctggc
    85261 gcacgatccc tgaccagttc cagcgcctga cgcattttgc tcgacgacgg gcagtcagaa
    85321 gaaccaaagt tggagtgcga caacagagca acgcgcggct caataccaaa acgacggaca
    85381 gtttctgccg ccatcaaggt gatctccgcc agctcttctg cgtctggttc gtcattgacg
    85441 taggtatcgg caataaaggt gttaccactc ggcagcagca gcgcattcat tgcacctgcg
    85501 gtgtgaacgc catcgcgata accaaagaca tttttcacca cactaaaatg ttcatgataa
    85561 tcgcccaccg taccgcagat cattgcatcg gcttccccac gctgaaccat gatcgcgccg
    85621 atcactgtcg ggttactgat cagcgcacgc tgcgcctgtt cctgagtgac accgcgacgc
    85681 ttcatgatct ggaagtattc ggtccagtac tctttaaagc gcggatcgga ttcgttattg
    85741 acgatttcaa aatcaacgcc cgctttgatc tgcaagccca gtttctgaat gcgcatttcg
    85801 atcacgttcg gacgaccgat aaggatcggt ttcgccagtc ccagcgttac cagttcctga
    85861 gtcgcatgca gaacgcgcgc ctcttcccct tccggcagaa ctacgcgctt cggcgctttg
    85921 cgagcctggg agaaaatcgg cttcataaac aggttggttt tgtaaacgaa ctcagtcagc
    85981 ttgtcgatgt agacgtcgaa atcagcaatc ggacgcgtcg ccacgcccga ctccatcgcc
    86041 gcttttgcga ccgcaggagc gatcttaacg atcaagcgcg gatcaaacgg ttttggaatg
    86101 atgtattccg gaccaaagct cagatcctga acgccatacg ctgaagccac cacttcactc
    86161 tgttctgcat gggccagttc tgcaatcgcg cgtaccgccg ccagtttcat ctcttcgttg
    86221 atggcggttg cgccaacgtc cagcgcgcca cggaagatga acgggaagca aagaacgttg
    86281 ttcacctggt tcgggtagtc ggaacggccg gtacagatga tggcatccgg acgcacttct
    86341 ttcgccagcg gcggcagaat ttccggttcc gggttcgcca gcgccaggat cattggtgcc
    86401 cgagccattt tcttcaccat ttcctgggtc agcactttcg ggccggaaca gcccaggaaa
    86461 atatccgcac cttcaatcac atcatcgagg gtacgtttgc cgtcatccac caccgcatat
    86521 gcggctttgg tttccgccat atttggctca cggccctgat agataacgcc ttttgaatcg
    86581 caaaccacga tgttatgttt ttgcagcccc agcgctacca gcaggttcat acaggcgatt
    86641 gctgcggcac ccgcgccgga aaccaccatc cgcacgtcgg agatgttttt ctccaccacg
    86701 cgcaagccgt tgaggatggc ggcagtgctg atgattgccg tgccgtgctg atcgtcgtgg
    86761 aataccggaa tattcatccg ctcgcgcagt ttctgttcaa tatagaaaca ttctggcgct
    86821 ttaatgtctt cgaggttgat gccgccgaag gtcggttcga gcgcggcgac gacttcaata
    86881 aatttgtccg ggtcgagttc atcaacttca atgtcaaata catcaatccc agcgaatttc
    86941 ttaaatagaa cgcccttgcc ttccatcacc ggtttgcctg ccagcgcgcc aatgttgcct
    87001 aaccccagca ccgccgtacc gttagagatc accgcaacca ggttaccgcg cgcggtatat
    87061 ttgtaggctt ttagcgggtc tttttcgatt tcaagacaag gtgcggcaac gcctggtgag
    87121 taggccagcg ccagatcgcg ctgcgttgcc agaggcttgg ttggagaaac ctagattttc
    87181 cctggaactg gaaattcatg gaaatcaagt gcactttgtt ttaactggtc atccatttgg
    87241 ttgttccttt cacgtaacgt tcacaaataa agtgtgtggg caacagcccc tgcccacaac
    87301 gtggcgcaca ttattaccct gccggagtct acagactttg agcaagtcca aactctcacc
    87361 attaatataa tgttttggta ataatcctat aacactgatg ttacctgctt aatccagcaa
    87421 taccatgcct gtctgctatg cttttttgat gcgtttagcg aaatttctca gaagtgtgaa
    87481 ttaacgcact catctaacac tttacttttc aaggagtatt tcctatgaac gagttagacg
    87541 gcatcaaaca gttcaccact gtcgtggcag acagctgcga tattgagtcc attcgccatt
    87601 atcatcccca ggatgccacc actaatcctt cgctgttact caaggctgcc ggattatcac
    87661 aatatgagca tttaatagac gatgctatcg cctggggtaa aaaaaatggc aagacccagg
    87721 aacaacaggt ggtcgcagcg tgtgacaaac tggcggtcaa tttcggtgct gaaattctca
    87781 aaatcgtacc cggttgcgtg tcaacagaag ttgatgcacg cctctctttt gataaagaaa
    87841 agagtattga gaaggcgcgc catctggtgg acttgtatca gcaacaaggc gttgagaaat
    87901 cacgcatttt gatcaaactg gcttcgacct gggaaggaat tcgcgcggcc gaagagctgg
    87961 aaaaagaagg tattaactgc aacctgacgc tgctgttttc ttttacccag gcgcgggcct
    88021 gtgcggaagc aggcgttttt ctgatttcgc cgtttgtagg gcgtatttat gactggtatc
    88081 aggcacgcaa gccgatggac ccgtatgtgg tggaagaaga tccgggcgtt aaatcggtgc
    88141 gcaatatcta cgactactat aagcaacacc actatgaaac cattgtgatg ggcgcgagct
    88201 tccgtcgcac cgaacaaatc ctcgccttaa ccggctgcga tcgactgact atcgcaccga
    88261 atttgctgaa ggagctgcag gaaaaagttt ctccagtggt acgtaaatta atcccgcctt
    88321 ctcagacgtt cccacgccca gcacccatga gcgaagcgga gttccgttgg gagcacaatc
    88381 aggatgcgat ggcggtagaa aaactgtctg aaggcattcg tctgttcgct gttgatcaac
    88441 gcaaactgga agatcttctt gccgccaaac tataaaccag ccacggagtg ttatatgtcc
    88501 cgaaaagacc ttgccaatgc gattcgcgca ctcagtatgg atgctgtaca aaaagccaat
    88561 tctggtcatc ccggcgcgcc gatgggcatg gctgatattg ccgaagtgct gtggaacgat
    88621 tttcttaaac ataatcctac cgacccaacc tggtatgatc gcgaccgctt tattctttcc
    88681 aacggtcacg cgtcgatgct gctctacagt ttgctacatc tgaccggtta cgacctgccg
    88741 ctggaagaac tgaagaactt ccgtcagttg cattcgaaaa ccccaggcca cccggagatt
    88801 ggctatacgc caggcgttga aaccaccacc ggcccgcttg gacaaggttt ggcgaacgcc
    88861 gtcgggctgg cgatagcaga gcgtacactg gcggcgcagt ttaaccagcc agaccatgag
    88921 atcgtcgatc acttcaccta tgtgtttatg ggcgacggct gcctgatgga aggtatttcc
    88981 cacgaagtct gttcgctggc aggcacgctg ggactaggca agctgattgg tttttacgat
    89041 cacaacggta tttccatcga cggtgaaaca gaaggctggt ttaccgacga tacggcaaaa
    89101 cgttttgaag cctatcactg gcatgtgatc catgaaatcg acggtcacga tccgcaggcg
    89161 gtgaaggaag cgatccttga agcgcaaagc gtgaaagata agccgtcgct gattatctgc
    89221 cgtacggtga ttggctttgg ttcgccgaat aaagcaggta aggaagaggc gcacggcgca
    89281 ccactggggg aagaagaagt ggcgctggca cggcaaaaac tgggctggca ccatccgcca
    89341 tttgagatcc ctaaagagat ttatcacgcc tgggatgccc gtgaaaaagg cgaaaaagcg
    89401 cagcagagct ggaatgagaa gtttgccgcc tataaaaagg ctcatccgca actggcagaa
    89461 gagtttcccc gacggatgag cggtggttta ccgaaggact gggagaaaac gactcagaaa
    89521 tatatcaatg agttacaggc aaatccggcg aaaatcgcta cccgtaaggc ttcgcaaaat
    89581 acgcttaacg cttacgggcc gatgctgcct gagttgctcg gcggttcggc ggatctggct
    89641 cccagcaacc tgaccatctg gaaaggttct gtttcgctga aggaagatcc ggcgggcaac
    89701 tacattcact acggggtgcg tgaatttggc atgaccgcta tcgccaacgg catcgcgcac
    89761 cacggcggct ttgtgccgta taccgcgacg ttcctgatgt ttgttgaata cgcccgtaac
    89821 gccgcgcgga tggcggcact gatgaaagcg cggcagatta tggtttatac ccacgactca
    89881 attggcctgg gcgaagatgg tccgacgcac caggctgttg agcaactggc cagcctgcgc
    89941 ttaacgccaa atttcagcac ctggcgaccg tgcgatcaga tggaagcggc ggtgggctgg
    90001 aagctggcgg ttgagcgcca caacggaccg acggcactga tcctctcaag gcagaatctg
    90061 gcccaggtgg aacgtacgcc ggatcaggtt aaagagattg ctcgtggcgg ttatgtgctg
    90121 aaagacagcg gcggtaagcc agatattatt ctgattgcca ccggttcaga gatggaaatt
    90181 accctgcaag cggcagagaa attagcagga gaaggtcgca atgtacgcgt agtttccctg
    90241 ccctcgaccg atattttcga cgcccaggat gaggaatatc gggagtcggt gttgccttct
    90301 aacgttgcgg ctcgcgtggc ggtggaagca ggtattgccg attactggta caagtatgtt
    90361 ggtctgaaag gggcaattgt cgggatgacg ggttacgggg aatctgctcc ggcggataag
    90421 ctgttcccgt tctttggctt taccgccgag aatattgtgg caaaagcgca taaggtgctg
    90481 ggagtgaaag gtgcctgatg gtgatcgccg gatgctgtat gttcccctca ccctaaccct
    90541 ctccccagaa gggcgagggg acggtccgtg cgctttttag ggccgcatct gcgattttgt
    90601 ttaacgggtg atccacaacg tgggcctggc gtctggccca tgccagttat cgcaggtagg
    90661 ttctgcggcg taacgcacca ggcgaaaacg ctgaccgtca aagcgccagc gcgcctgaat
    90721 gccacaatcg cttaatccgc gccctttcgc taaggtcacc agttcacgcg atttctcatc
    90781 aaatgttgcg ttcatcagtt ccagttcatt cgtctcctgg ccgttgttga acggcaaacg
    90841 caaccgaacc gggcgcgagg ctagcggctt tttacgcgac acaatccatg ccaaatcaat
    90901 ggtgttatag gcccctgcct cacagctaat catcatcagt gctttatcat cggtcagcgc
    90961 agtgacattc acctcacgac gtaacggatc aagcgagcag cgcagaccat tcatccgcca
    91021 gtttccataa tccagcaaat cgttacgttc ttcgagtgag agtggcgtcg gcgttgggtt
    91081 aaccaccgcg acctctttca gcgcaggcgc gggcggtacg ctgagaggcg gttcgtcccc
    91141 tttcttgatc cacgcggttt cactgccaac gcgcttttgc tgagcatcaa taaacaacaa
    91201 cgccgctttt aagccgctca gagaaatggt ctgattgccg tcgcgtaagg tgatcgcctt
    91261 cccttcctga attatctgca aaaacgcggt gatggttgcc gtatcatcgg ttactaataa
    91321 ccatggtgaa atccgccact tgtcaccact taatgccaac ggctcaccat ctaatagcag
    91381 ccgtggcgct atctcccctt cagacgcatc cggcgacttc aacccaccgc gctcaatacg
    91441 taaaacggca tcggtatgcg ccccagcgct gcggctcagg gtcatcacca gtccattatg
    91501 atcgcccgta ttgcgcgcca cacaaaaatt ttgattatta caggtgacct gccagtcgga
    91561 aaacgcccgt tgcgctggtg ccgcccacac caaagacgtc ggcaacaaag cgaaaaaaaa
    91621 gagaaggaaa atgcgatagc gcatggacgg tacgacccca gaagttcaaa caaacagcac
    91681 aagtcgtatc ttcgttgcgc aggtagcgcg gctcaatcgg atttgtcgga ttgagccaat
    91741 aataaacaac gaaatattat tgctattttt cagtccatta aatgtgacat ttgcaaatag
    91801 ttaagcaata acaccgtctt accgtcacgt atctcgccgg ttttgatcat ctccagcgcc
    91861 tggctgaacg gcagctcgag cacttcaata tcttcatctt caacaccgcc accggcgtta
    91921 gcgcgctgat tgtcactgta ttcggcgata aaaaagtgga ttagctcagt cacaccgcca
    91981 ggcgacatat acagttcaaa taatttgcgc acttcgccca cttcatagcc cgtctcttca
    92041 atcgcttctt tacgaatgca cacttccggt tcgtcgttat ccagcagccc ggcgcaggtt
    92101 tcaatcagct gcccgctttc attgccatta acccaggtag cgacacggaa ctgacgaatc
    92161 agaaccacgc tctttttctt cgcgttgtac aggaggatcg tcgcaccatt accgcgatcg
    92221 taaacttcac gtttatggcg gataacttcg ccatctttgc gcgtgagatc gtaagtaatg
    92281 ttgtgcaggg tgaaatagtt atcggagaga attttgtctt taatgagggt gatttgttgc
    92341 gtcataccga ctccacagcg cgaaatgaac aattatatta cgctgtgaag tcgggtttgt
    92401 ctgcgcaggc tatcagtgag atttgactga ttttaccccc agccagtcaa taatcccctg
    92461 tgccgcatga cgtccttctg ccattgcggt aaccaccaga tccgcaccac gcacggcgtc
    92521 gccaccggcg aagattttcg gattggtggt ctggtaacgg tactggcttt ccacatccgc
    92581 gatgatgcgg ccccatttgt ctaccgttac accgtgcgac tccagccacg gcatcccgtg
    92641 tggattgaag ccaaacgcca taatcaccgc atcggctggc atgacaaatt cactgccctc
    92701 aatcggtaca ggtcgccgac gcccctgggc atccggctcg ccaagacgcg tgcgcaggaa
    92761 acgaattccg caaacgtgac cttgctcatt cagctcaagc gccaccggct ggacgttaaa
    92821 ttcgaagttg gccccctctt cgcgggcgtt cttcacctct ttcttcgagc ctggcatgtt
    92881 agcttcatca cgacgataag cgcaggtgac gttactcgcg ccgtggcgca gcgcagtacg
    92941 cacacagtcc atcgcggtgt cgccgccgcc cagtaccacg acgttaagtc cggcggtatt
    93001 gataaacggc tcttccggta gctcttcgag ccccatcacc tgtttagtgt tggcaatgag
    93061 gaacggcagc gcgtcataaa cgcccggcgc atcttcattg ggtaaacccg ctttcatgga
    93121 acggtaagtg cctacgccaa cgaagaccgc gtcgtattgt tccaaaagca aatccaaaga
    93181 gacatcttta cccacttcac aattgagttc gaagtgaatc cccatcgcgc tgaagatttc
    93241 ccgacggcgt gccagcaggg atttatccag tttgaaagaa ggaatgccga aagtgagcaa
    93301 gccaccgatt tctggatggc gatcgtacac cgtcaccgcc acgccattgc gggtcagaac
    93361 atccgcacag gccagccctg ccggacctgc cccgataatc gccacccgct tgtccacttt
    93421 ggtgacatga cttaagtcag gacgccaacc tttcgccaac gcctgatctg aaatatagcg
    93481 ttcaatgttg ccgatagtta ccgcgccgtg ctcatcgcga atagtacagg caccttcaca
    93541 caaacggtct tgcggacaaa cgcgtccggt aatttccggc agggtgttgg tctggtgaga
    93601 aagctcgacg gcggcgtcga tgtttccggc tttcaccagt tcaatccact gcggaatatg
    93661 gttatgcagc gggcaggtcc attcacaaac gctgtgctcg ccgaacttaa ggcagcgcga
    93721 ggcttcccgt tgtgcctggt cggcgcgaaa tggcagataa atttcatcaa aaccggtttt
    93781 gcgcgcttca atcgccagtt tatccggctc gccacgcgcg ggcgttgcct gcatttgttc
    93841 gactttactc attaccggca tttcttgtgc agcggtactg gcatgccacg gttgatgctc
    93901 ctgacgtgcg gtgcgcaagc ggcgggattt cgccatgccg gagagtgcga cgtcagtgac
    93961 cagttgcagc gcgtccgccg ggcaattctc gacacaggca ggaccgtttt cgcgccctgc
    94021 acaaaggtcg catttatgcg ccgtggcttt tacttttcct gccgcgacgg gtgtcaggac
    94081 gatttgcatc gtaccaaacg gacaggccac cacgcaggat ttacagccaa tacacttttg
    94141 ctgattgacc tgaatgctgt catcaacgtg gctgattgcg ccattagggc agctacgggc
    94201 gcagggcgca tcttcacaat ggtgacaggt cactgcacta cgttgctgct gatgtttgat
    94261 aaccgtaatt cggggatgaa aatggtgttg gctcaggaca tgttgctcat cattgtgagc
    94321 catgacacag gcgatttcac aagcatgaca acccagacat tgctgactgt tggccataat
    94381 aaaacgattc ataacgacct tcttttttgg ttgtaaaaac cttattcttt atatgagtgt
    94441 tgttattacc cgacttacag gggaaccggc aatgttcatg tgcccagaat aagtaactat
    94501 t
//
LOCUS       DAETQL010000003        75634 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.003, whole genome shotgun sequence.
ACCESSION   DAETQL010000003 DAETQL010000000
VERSION     DAETQL010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.003"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(435..641)
                     /locus_tag="K0W65_000200"
     CDS             complement(435..641)
                     /locus_tag="K0W65_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBI3093626.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="K0W65_000201"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="K0W65_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBI3093627.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="K0W65_000202"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="K0W65_000202"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBI3093628.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="K0W65_000203"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="K0W65_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="K0W65_000204"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="K0W65_000204"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBI3093629.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="K0W65_000205"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="K0W65_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8722
                     /gene="kdpE"
                     /locus_tag="K0W65_000206"
                     /pseudo
     CDS             8037..8722
                     /gene="kdpE"
                     /locus_tag="K0W65_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8997..9101
                     /locus_tag="K0W65_000207"
     CDS             8997..9101
                     /locus_tag="K0W65_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBI3093630.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9412..11609
                     /gene="speF"
                     /locus_tag="K0W65_000208"
                     /pseudo
     CDS             9412..11609
                     /gene="speF"
                     /locus_tag="K0W65_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11606..12925
                     /gene="potE"
                     /locus_tag="K0W65_000209"
     CDS             11606..12925
                     /gene="potE"
                     /locus_tag="K0W65_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBI3093631.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMAPAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="K0W65_000210"
     CDS             complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="K0W65_000210"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBI3093632.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLTDI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="K0W65_000211"
     CDS             complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="K0W65_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBI3093633.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15377..16141
                     /gene="ybfF"
                     /locus_tag="K0W65_000212"
     CDS             15377..16141
                     /gene="ybfF"
                     /locus_tag="K0W65_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBI3093634.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16281..16574
                     /gene="ybfE"
                     /locus_tag="K0W65_000213"
     CDS             16281..16574
                     /gene="ybfE"
                     /locus_tag="K0W65_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBI3093635.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16714..17244
                     /gene="fldA"
                     /locus_tag="K0W65_000214"
     CDS             16714..17244
                     /gene="fldA"
                     /locus_tag="K0W65_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBI3093636.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17454..17540
                     /gene="uof"
                     /locus_tag="K0W65_000215"
     CDS             17454..17540
                     /gene="uof"
                     /locus_tag="K0W65_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBI3093637.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17533..17979
                     /gene="fur"
                     /locus_tag="K0W65_000216"
     CDS             17533..17979
                     /gene="fur"
                     /locus_tag="K0W65_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBI3093638.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18063..18389)
                     /locus_tag="K0W65_000217"
     CDS             complement(18063..18389)
                     /locus_tag="K0W65_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093639.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="K0W65_000218"
     CDS             complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="K0W65_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HBI3093640.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20411..22075)
                     /gene="glnS"
                     /locus_tag="K0W65_000219"
     CDS             complement(20411..22075)
                     /gene="glnS"
                     /locus_tag="K0W65_000219"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBI3093641.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22278..24224)
                     /gene="nagE"
                     /locus_tag="K0W65_000220"
     CDS             complement(22278..24224)
                     /gene="nagE"
                     /locus_tag="K0W65_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBI3093642.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24557..25357
                     /gene="nagB"
                     /locus_tag="K0W65_000221"
     CDS             24557..25357
                     /gene="nagB"
                     /locus_tag="K0W65_000221"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBI3093643.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25417..26565
                     /gene="nagA"
                     /locus_tag="K0W65_000222"
     CDS             25417..26565
                     /gene="nagA"
                     /locus_tag="K0W65_000222"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBI3093644.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26574..27794
                     /gene="nagC"
                     /locus_tag="K0W65_000223"
                     /gene_synonym="nagR"
     CDS             26574..27794
                     /gene="nagC"
                     /locus_tag="K0W65_000223"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBI3093645.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27842..28594
                     /gene="nagD"
                     /locus_tag="K0W65_000224"
                     /pseudo
     CDS             27842..28594
                     /gene="nagD"
                     /locus_tag="K0W65_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            28991..30655
                     /gene="asnB"
                     /locus_tag="K0W65_000225"
     CDS             28991..30655
                     /gene="asnB"
                     /locus_tag="K0W65_000225"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBI3093646.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31034..31110
                     /locus_tag="K0W65_000226"
     tRNA            31034..31110
                     /locus_tag="K0W65_000226"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31068..31070,aa:Met,seq:cat)
     gene            31119..31203
                     /locus_tag="K0W65_000227"
     tRNA            31119..31203
                     /locus_tag="K0W65_000227"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31153..31155,aa:Leu,seq:tag)
     gene            31227..31301
                     /locus_tag="K0W65_000228"
     tRNA            31227..31301
                     /locus_tag="K0W65_000228"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31259..31261,aa:Gln,seq:ttg)
     gene            31336..31410
                     /locus_tag="K0W65_000229"
     tRNA            31336..31410
                     /locus_tag="K0W65_000229"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31368..31370,aa:Gln,seq:ttg)
     gene            31426..31502
                     /locus_tag="K0W65_000230"
     tRNA            31426..31502
                     /locus_tag="K0W65_000230"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31460..31462,aa:Met,seq:cat)
     gene            31551..31625
                     /locus_tag="K0W65_000231"
     tRNA            31551..31625
                     /locus_tag="K0W65_000231"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31583..31585,aa:Gln,seq:ctg)
     gene            31663..31737
                     /locus_tag="K0W65_000232"
     tRNA            31663..31737
                     /locus_tag="K0W65_000232"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31695..31697,aa:Gln,seq:ctg)
     gene            complement(31891..33066)
                     /gene="ubiF"
                     /locus_tag="K0W65_000233"
     CDS             complement(31891..33066)
                     /gene="ubiF"
                     /locus_tag="K0W65_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBI3093647.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33212..34636
                     /gene="miaB"
                     /locus_tag="K0W65_000234"
     CDS             33212..34636
                     /gene="miaB"
                     /locus_tag="K0W65_000234"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBI3093648.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDASHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34789..35829
                     /locus_tag="K0W65_000235"
     CDS             34789..35829
                     /locus_tag="K0W65_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBI3093649.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35826..36293
                     /gene="ybeY"
                     /locus_tag="K0W65_000236"
     CDS             35826..36293
                     /gene="ybeY"
                     /locus_tag="K0W65_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBI3093650.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36383..37261
                     /gene="corC"
                     /locus_tag="K0W65_000237"
     CDS             36383..37261
                     /gene="corC"
                     /locus_tag="K0W65_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBI3093651.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37286..38824
                     /gene="lnt"
                     /locus_tag="K0W65_000238"
     CDS             37286..38824
                     /gene="lnt"
                     /locus_tag="K0W65_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBI3093652.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39221..40129
                     /gene="gltI"
                     /locus_tag="K0W65_000239"
     CDS             39221..40129
                     /gene="gltI"
                     /locus_tag="K0W65_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HBI3093653.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40299..41039
                     /gene="gltJ"
                     /locus_tag="K0W65_000240"
     CDS             40299..41039
                     /gene="gltJ"
                     /locus_tag="K0W65_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBI3093654.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            41039..41713
                     /gene="gltK"
                     /locus_tag="K0W65_000241"
     CDS             41039..41713
                     /gene="gltK"
                     /locus_tag="K0W65_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBI3093655.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41713..42438
                     /gene="gltL"
                     /locus_tag="K0W65_000242"
     CDS             41713..42438
                     /gene="gltL"
                     /locus_tag="K0W65_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBI3093656.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42556..43491
                     /gene="rihA"
                     /locus_tag="K0W65_000243"
     CDS             42556..43491
                     /gene="rihA"
                     /locus_tag="K0W65_000243"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBI3093657.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43575..45245
                     /gene="hscC"
                     /locus_tag="K0W65_000244"
                     /pseudo
     CDS             43575..45245
                     /gene="hscC"
                     /locus_tag="K0W65_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45305..46756)
                     /gene="djlC"
                     /locus_tag="K0W65_000245"
     CDS             complement(45305..46756)
                     /gene="djlC"
                     /locus_tag="K0W65_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HBI3093658.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46753..47460)
                     /locus_tag="K0W65_000246"
     CDS             complement(46753..47460)
                     /locus_tag="K0W65_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBI3093659.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47562..48116
                     /locus_tag="K0W65_000247"
     CDS             47562..48116
                     /locus_tag="K0W65_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBI3093660.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48126..49553)
                     /locus_tag="K0W65_000248"
     CDS             complement(48126..49553)
                     /locus_tag="K0W65_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HBI3093661.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49550..50257)
                     /locus_tag="K0W65_000249"
     CDS             complement(49550..50257)
                     /locus_tag="K0W65_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBI3093662.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50421..51398
                     /locus_tag="K0W65_000250"
     CDS             50421..51398
                     /locus_tag="K0W65_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBI3093663.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51468..51950)
                     /locus_tag="K0W65_000251"
     CDS             complement(51468..51950)
                     /locus_tag="K0W65_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBI3093664.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52185..54767
                     /gene="leuS"
                     /locus_tag="K0W65_000252"
     CDS             52185..54767
                     /gene="leuS"
                     /locus_tag="K0W65_000252"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBI3093665.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54782..55363
                     /gene="lptE"
                     /locus_tag="K0W65_000253"
     CDS             54782..55363
                     /gene="lptE"
                     /locus_tag="K0W65_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBI3093666.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55363..56394
                     /gene="holA"
                     /locus_tag="K0W65_000254"
     CDS             55363..56394
                     /gene="holA"
                     /locus_tag="K0W65_000254"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBI3093667.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56396..57037
                     /gene="nadD"
                     /locus_tag="K0W65_000255"
     CDS             56396..57037
                     /gene="nadD"
                     /locus_tag="K0W65_000255"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBI3093668.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57061..57672
                     /locus_tag="K0W65_000256"
     CDS             57061..57672
                     /locus_tag="K0W65_000256"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HBI3093669.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57932..58249
                     /gene="rsfS"
                     /locus_tag="K0W65_000257"
                     /gene_synonym="rsfA"
     CDS             57932..58249
                     /gene="rsfS"
                     /locus_tag="K0W65_000257"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBI3093670.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58253..58720
                     /gene="rlmH"
                     /locus_tag="K0W65_000258"
     CDS             58253..58720
                     /gene="rlmH"
                     /locus_tag="K0W65_000258"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBI3093671.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58751..60652
                     /gene="mrdA"
                     /locus_tag="K0W65_000259"
     CDS             58751..60652
                     /gene="mrdA"
                     /locus_tag="K0W65_000259"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBI3093672.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60655..61767
                     /gene="mrdB"
                     /locus_tag="K0W65_000260"
                     /gene_synonym="rodA"
     CDS             60655..61767
                     /gene="mrdB"
                     /locus_tag="K0W65_000260"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBI3093673.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61778..62866
                     /gene="rlpA"
                     /locus_tag="K0W65_000261"
     CDS             61778..62866
                     /gene="rlpA"
                     /locus_tag="K0W65_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBI3093674.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            63005..64216
                     /gene="dacA"
                     /locus_tag="K0W65_000262"
     CDS             63005..64216
                     /gene="dacA"
                     /locus_tag="K0W65_000262"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBI3093675.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64327..64590
                     /locus_tag="K0W65_000263"
     CDS             64327..64590
                     /locus_tag="K0W65_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBI3093676.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64691..65332
                     /gene="lipB"
                     /locus_tag="K0W65_000264"
     CDS             64691..65332
                     /gene="lipB"
                     /locus_tag="K0W65_000264"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBI3093677.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65591..66544
                     /locus_tag="K0W65_000265"
     CDS             65591..66544
                     /locus_tag="K0W65_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBI3093678.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66753..67718
                     /gene="lipA"
                     /locus_tag="K0W65_000266"
     CDS             66753..67718
                     /gene="lipA"
                     /locus_tag="K0W65_000266"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBI3093679.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67819..68022)
                     /gene="tatE"
                     /locus_tag="K0W65_000267"
     CDS             complement(67819..68022)
                     /gene="tatE"
                     /locus_tag="K0W65_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBI3093680.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68151..68939)
                     /locus_tag="K0W65_000268"
     CDS             complement(68151..68939)
                     /locus_tag="K0W65_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBI3093681.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69032..69415
                     /gene="crcB"
                     /locus_tag="K0W65_000269"
     CDS             69032..69415
                     /gene="crcB"
                     /locus_tag="K0W65_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBI3093682.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69469..69678)
                     /gene="cspE"
                     /locus_tag="K0W65_000270"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69469..69678)
                     /gene="cspE"
                     /locus_tag="K0W65_000270"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBI3093683.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69853..70413)
                     /gene="pagP"
                     /locus_tag="K0W65_000271"
     CDS             complement(69853..70413)
                     /gene="pagP"
                     /locus_tag="K0W65_000271"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HBI3093684.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            71002..72387
                     /gene="dcuC"
                     /locus_tag="K0W65_000272"
     CDS             71002..72387
                     /gene="dcuC"
                     /locus_tag="K0W65_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBI3093685.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(72428..72769)
                     /locus_tag="K0W65_000273"
                     /pseudo
     CDS             complement(72428..72769)
                     /locus_tag="K0W65_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73107..73616)
                     /locus_tag="K0W65_000274"
     CDS             complement(73107..73616)
                     /locus_tag="K0W65_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBI3093686.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73779..74024
                     /locus_tag="K0W65_000275"
     CDS             73779..74024
                     /locus_tag="K0W65_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3093687.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            73999..75630
                     /locus_tag="K0W65_000276"
                     /pseudo
     CDS             73999..75630
                     /locus_tag="K0W65_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
BASE COUNT        18617 a        18994 c        19208 g        18815 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggctatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggaattacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttattgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ctccgccacc gccgcgcccg atccgctggt gaaattttcc ggtgttaccg tcgatttagc
     8461 cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct
     8521 gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca
     8581 ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct
     8641 gcgacaaaaa ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat
     8701 tgggtatcgg tttatgcttt gaattgttat tcaataaatt cgcttaaata gtgattaatt
     8761 tattattaaa cctgcgccga atattattat ttggcaggtt taatttctta acgcaaatat
     8821 aaaacacgaa attacaacat ttaagtaaca aaacagcatc taatatggcg atattcataa
     8881 ttaatgattt attatctggg gtatgatcgt tttttgttga tcttcttcac agattatagc
     8941 catttcatgg atagaataac tctaccttca actgacacag caagcggtaa aggtaaatgg
     9001 aaaataataa ccgcttaatg cctcatataa ggcggacaac ccatatcatg aagtttgccc
     9061 atcgtaatag ttttgacttt catttcttta atgcccgcta gtcttctgac taaagggcac
     9121 cccaacgtac aggtctccct gactttaagc attacaggtt aatacctgta ttcctcggtg
     9181 ctcatatact gctaaccctt ttaaactcta aataattcga gtcgcagcac ttgcaacttg
     9241 aggtatgacg agtatagcca gttaccgggc tggtctgggt tattgcatct gcaaaaagca
     9301 aactactgat ttatttatca gcggtggagc tttgcttttt ttccgacgtg atcgatttct
     9361 cctttgagaa attgaggacc tgctattacc tgaaataaag agatgaataa aatgtcagaa
     9421 ttaaaaattg ccgttagccg ttcttgcccg gattgttttt ccactcatcg tgcatgcgtg
     9481 aatattgacg aaagtaatta tattgacgtt gccgccatta ttttatcagt caatgatgtt
     9541 gaacgtggaa aactcgataa aatagacgct actggctatg gcattcctgt ttttattgca
     9601 acggaaaatg aagaacgtgt accagcagaa tatctgccac gtatttctgg tgtcttcgag
     9661 cattgcgaat cacggaaaga gttttatgga cgtcagttag aaaccgctgc tagccattat
     9721 gaaacgcaac tgcgcccgcc cttcttccgc gcactggtcg attatgtcaa tcagggcaat
     9781 agcgcttttg actgcccggg tcatcaggga ggtgagtttt tccgtcgtca tccggcaggg
     9841 aatcagtccg tggagtactt cggagaaacg ctgttccgtt ccgacttatg caacgcagac
     9901 gtagcaatgg gcgatctgct gattcacgaa ggcgcgccat gcattgcaca gcaacatgcg
     9961 gcaaaagtgt ttaatgccga taaaacatac ttcgttctaa atggcacttc atcttctaac
    10021 aaagtggttt taaacgccct gctaacaccg ggtgatctgg tgctgtttga tcgcaataac
    10081 cacaaatcta accaccacgg agcgttgcta caggctggtg caacaccggt ttatctggaa
    10141 acggcacgta acccgtatgg ctttatcggt ggcattgatg cgcactgttt tgaagaaagt
    10201 tacctgcgtg agctgatcgc ggaagtcgca ccgcagcggg caaaagaggc tcgtcctttc
    10261 cgcctcgctg tgattcagtt aggcacctac gacggtacga tttataacgc ccgccaggtg
    10321 gtggataaaa ttggtcatct gtgtgactac attctgtttg actccgcatg ggttggctat
    10381 gagcaattta tcccgatgat ggctgactgt tcgccgctgt tgctggatct taatgagaac
    10441 gatccgggta ttctagttac gcaatccgta cataaacagc aggctggttt ttctcaaact
    10501 tcacaaatcc ataaaaaaga cagccacatc aaagggcaac agcgttatgt accgcacaaa
    10561 cgcatgaaca acgcctttat gatgcacgcc tccaccagcc cgttctatcc gctgtttgcc
    10621 gcactggata tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat gtggatggat
    10681 tgtgtggtaa atggcattaa tgcccgcaaa ctgatcctcg ataactgtca gcatattcgt
    10741 ccgttcgtac ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt
    10801 gcggttgatc tgcgcttctt ccagtttgta ccaggggaac actggcattc ttttgaaggc
    10861 tatgcagaga atcaatactt tgtcgatcct tgcaaactgt tgctgacaac cccaggtatt
    10921 gatgcacgta acggcgaata tgaagcgttc ggtgtacccg cgacgattct tgctaacttc
    10981 ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta acgccatcct cttcctgctg
    11041 actccggcag aagatatggc caaacttcag caacttgtgc cctgctggta cgcttcgaaa
    11101 aactgcttga gtccgacgcg ccattagcag aagtgctgcc ttccatctac aaacagcatg
    11161 aagagcgcta cgccggttat accctgcgtc agttgtgcca ggaaatgcat gatttgtatg
    11221 cccgccacaa cgtgaaacaa ctgcaaaaag agatgttccg caaagcgcac ttcccacacg
    11281 tcagcatgaa tccgcaagaa gccaactacg cctatttacg cggtgaagtg gagctggttc
    11341 gtctaccgga tgccgaaggt cgtatcgctg ccgaaggggc gcttccttat cctccgggtg
    11401 tgctgtgtgt tgttccgggt gaaatctggg gtggtgctgt tttgcgttac ttcagcgctc
    11461 tggaagaagg gatcaacctg ctgccaggtt ttgcaccgga gctgcagggt gtctatatcg
    11521 aagaacatga tggtcgtaag caagtttggt gctatgtcat caagcctcgt gatgcgcaaa
    11581 gcaccctgtt gaaaggggaa aaattatgag tcaggctaaa tcgaacaaaa tgggcgtcgt
    11641 tcagttaacc atactgacga tggtcaacat gatgggctcc ggtatcatca tgctgccgac
    11701 aaagcttgcc gaagttggga caatttcaat tatctcctgg ctggtgacag ccgtaggctc
    11761 aatggcaccg gcatgggcat tcgcaaaatg cggtatgttc agccgtaaat caggtggtat
    11821 gggcggttat gccgagtatg ctttcggtaa atccggtaac tttattgcga actataccta
    11881 cggcgtctca ctgctgattg ctaacgtcgc gattgctatt tcggcggttg gttacggcac
    11941 cgaactgctc ggcgcgagtt tgtcgccagt gcagattggt cttgcgacca tcggggtgct
    12001 gtggatttgt accgtggcta actttggtgg tgcgcgcatt accgggcaaa tcagtagcat
    12061 taccgtgtgg gggggcatta ttccggtggt aggcctgtgc attatcggct ggttctggtt
    12121 tagcccgacg ctttacgttg attcctggaa tccgcatcat gcaccgttct tcagtgcagt
    12181 aggttcttcc atcgctatga cgctgtgggc ttttcttggt ctggagtctg cgtgtgcgaa
    12241 tactgatgta gtggaaaacc cggaacgtaa tgtgccaatc gcggtactcg gcggtacgtt
    12301 aggtgcggcg gtgatttata tcgtctccac caacgtgatt gcagggattg tgcctaatat
    12361 ggagctggca aattcaacgg caccatttgg cctggccttc gcacagatgt ttacaccaga
    12421 agtgggtaaa gtcattatgg cgctgatggt gatgtcctgc tgcggttcgc tgcttggctg
    12481 gcagttcacc attgcccagg tgtttaaatc ttcatctgat gaaggctact tccctaaaat
    12541 tttctcccgt gtaaccaaag tggatgcacc ggtgcaggga atgttgacca ttgtgattat
    12601 ccagagcggg ttggcgctga tgacgattag cccgtcgcta aacagtcagt tcaacgtgct
    12661 ggttaacctg gccgtggtga ccaatatcat tccgtatatt ctgtcaatgg cggcattagt
    12721 cattattcag aaggtcgcta atgtgccccc ctcaaaagcg aaagttgcaa actttgttgc
    12781 ttttgttggc gcgatgtata gcttttatgc gctgtactca tccggggaag aagccatgct
    12841 gtacggttcc atcgtgacct tccttggttg gacactgtat ggtctggtat caccacgctt
    12901 tgaactgaaa aataaacacg gttaaaaaag caaagtaaaa aagggcgatc ttgcgaccgc
    12961 tcttttttta ttaaatgtgt ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct
    13021 tctttctcaa tctgcttgcg atgttcttca ccgaggaagc tttcgcagta gatcttatat
    13081 gcgtcttccg tgcctgacgg acgcgcggcg aaccagccgt tgtcagtcat cactttcaga
    13141 ccgccaatag aagcaccgtt gcccggagca gcagtcaggc gcgcggtgat cgggtcacct
    13201 gccagggtgc tggcgctcac catttccgga gacagcttag acagcgccgc tttttgtgcg
    13261 gaagtcgcag ctgcctgcaa acggttgtag ctcggcgcac caaagcgttt tgccagttcg
    13321 ttgtagtgtt cctgcgggtt cttaccggtg acagcggtga tttccgccgc cagcagacac
    13381 ataatgatgc cgtctttgtc ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc
    13441 cctgcgctct cttcgccgcc aaagccgaag ctgccgtcga acagaccatc aacaaaccat
    13501 ttgaaaccta ccggtacttc taccagctta cggcccaagt cattgaccac acggtcgatc
    13561 atcgcagaag aaaccagcgt tttaccgaca gcaacatctt tgccccactg cggacgatgc
    13621 tggaacaggt agttgatcgc caccgccagg tagtggttcg gattcatcaa acctgccgga
    13681 gtgacgatac cgtgacggtc ataatccggg tcgttagcaa acgccagatc gaacttatca
    13741 cgcagtgcca gcagacccgc catcgcacac tcggaggagc agtccatacg gatcgcaccg
    13801 tctttatcga ggtgcataaa gcggaaggtt tgatcgacct gatcgttaac gatggtcagg
    13861 ttgaggttgt aatactcgcc aatgcgcttc cagtattcga taccggaacc gcccaacgga
    13921 tcaacgccca gcgtcaggcc cgctttctga atcgccgcca tatcaacgat atcggtcagc
    13981 ccttccacga acggctgcac cagatcctgc tctttcacat agccggatgc catcgcttcg
    14041 tcgagggaga tacgcttcac gcctttcagg ccatcggcca tcagtgcgtt ggccttgtct
    14101 tccaccactt tggtgacgtt agtatcagcc gggccaccat ttggcggatt gtacttgata
    14161 ccaccatctt ccggcgggtt atgggacggt gtaatcacga taccgtctgc cagcgggcca
    14221 ccttttttat tgtgaaccag gatggcattg gaaacggcag gcgtcggagt gaagccattg
    14281 ttttcctgca caatgacatc aacgccgttc gctgccagca cttccagtac ggaaatgaac
    14341 gcaggctcgg acagggcgtg agtatcttta cccacatagc aagggccagt gatgccgttt
    14401 ttcgcacgtt cttcagcaat tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg
    14461 tggcgcgctg cactgccacg gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc
    14521 cctgcttctg gtttcagtac ataatattgc gccgtcagtt gggcgacgtt aatcaaatca
    14581 ctctgttgtg caggttggcc tgcacgattg tggattgcca ttgctttgtc ctttgtctgc
    14641 aacgttttag atagttccgc aaaccttctc aatcaattcc gccgggaatt gcatcgactg
    14701 catgatgtgt tcgatcatgc tgcatttacg gccggtgttg gtgttggtga tcacccaata
    14761 cggcgtgcct ggcacatgtt tcggcttggt ctgattacca tttttcagca gcgtttgttc
    14821 atctgccgca aagtacacgc gtgtacgacc gtgcaacgat tccgttgctt cggcaaacgc
    14881 ctgggcgtca agagaatata gtgtagacaa cagcagcata aagcgattga ccgctcgctt
    14941 ttgctctgcg tattcgtccg aaagcagaag ttcacgcatt gcgcgaacct tgtctttaat
    15001 cgttttgacc ggcttcgctt cgacgatagc aggtgacgca acgcgaacct ctttcgtcac
    15061 cggagcagca ggctgtgatg cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc
    15121 gctctcgccg atatgcttag tgtggctggc aatatagctg tagagttcat catcaacttc
    15181 aatcgttttc atcttaatcc agtgcagtgt cttagctgaa tacaagtcgc caagagtata
    15241 tgaataaatc ccggcagcgg atagcgtcaa gcctggcttg cagaaaaact cggaataaac
    15301 cttatttgtt gcagccctgt ggcacaatgg tcaacataat accctaaccc gacaacgcga
    15361 aaaaaagaac tttgccatga aattaaatat ccgcgcgcaa actgcacaaa accagcacaa
    15421 taattctccc atcgttcttg tccatggtct gtttggtagc ctcgacaacc ttggcgtact
    15481 ggctcgcgat ctggtaaacg atcacaatat catccaggtt gatatgcgta accacggtct
    15541 ttcaccgaga gagccggtaa tgaattaccc ggcgatggcg caggatcttg ttgataccct
    15601 ggatgcactg cagatcgaca aagcaacatt tatcggtcac tccatgggcg gtaaagcggt
    15661 aatggcactt actgcactgg cccccgatcg catcgataaa ctggtggcga tcgatatcgc
    15721 accggtcgac tatcacgtac gccgtcatga tgagattttc gcagctatca acgcggtcag
    15781 tgaatcggac gcacaaactc gccagcaagc agcagcaata atgcgccagc atcttaatga
    15841 agaaggggtg attcagtttc tgttgaaatc ttttgttgac ggggagtggc gctttaacgt
    15901 gccggtattg tgggatcagt atccgcatat tgtgggttgg gagaaaatcc cggcatggga
    15961 tcaccctgcc ctgtttatcc ctggcggcaa ttctccgtat gttagcgagc agtaccgtga
    16021 tgatttactg gcacaatttc cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg
    16081 ggtccatgct gaaaaaccgg atgcggtatt acgcgctatc cgtcgctatc tcaatgatta
    16141 acgcaactga ttaaaaaccc agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac
    16201 tcatcgcgct gatgtattat ggcgcgctat ccatccgggc agaggcctgg ctgattgttt
    16261 cccccgaagt caccaagatc atggccaaag aacaaacgga ccgtacgaca ttagatctgt
    16321 tcgcgcacga gcgtcgaccg ggacgaccga aaactaatcc gctttcgcgc gatgaacagc
    16381 tgcgtattaa taaacgcaac cagctaaaac gcgacaaagt acgtggcctt aagcgtgtcg
    16441 aactgaagct gaacgcggaa gctgtcgagg cgctgaacga gctggcggaa tcgcgtaata
    16501 tgagccgtag cgaactgatc gaagagatgt taatgcagca actggcggcg ctgcgtagtc
    16561 agggcatcgt ttaaatttcc actttcatgt agcacagtgt gcagtcctgc tcgtttgctg
    16621 attccgcaag actgcctgtt ctgctatgat tgcctttatc cgtgggcaat tttccacccc
    16681 catttcaata agtttcaaga ggttatttca ctcatggcta tcactggcat ctttttcggc
    16741 agcgacaccg gtaataccga aaatatcgca aaaatgattc aaaaacagct tggtaaagac
    16801 gttgccgatg tccatgacat tgcaaaaagc agcaaagaag atctggaagc ttacgacatt
    16861 ctgctgctgg gcatcccaac ctggtattac ggcgaagcgc agtgtgactg ggatgacttc
    16921 ttcccgactc tcgaagagat tgatttcaat ggtaaactgg ttgcgctgtt tggttgtggt
    16981 gaccaggaag attacgccga atatttctgc gacgcattgg gcaccatccg cgacatcatt
    17041 gaaccgcgcg gtgcaaccat cgttggtcac tggccaactg cgggctatca tttcgaagca
    17101 tcaaaaggtc tggcagatga cgaccacttt gtcggtctgg ctatcgacga agaccgtcag
    17161 ccggaactga ccgctgaacg tgtagaaaaa tgggttaaac agatttctga agagttgcat
    17221 ctcgacgaaa ttcttaatgc ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta
    17281 atagataatg ccaatcaaaa taattgctac aaatttgtaa cttttgctgt tgtacctata
    17341 caatgtccca gtgttcaagt ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat
    17401 taacaatatt tgccagggac ttgtggtttt catttaggcg tggcaattct ataatgatac
    17461 gcattatctc aagagcaaat tctgtcactt cttctaatga agtgaaccgc ttagtaacag
    17521 gacagattcc gcatgactga taacaatacc gccctaaaga aagctggcct gaaagtaacg
    17581 cttcctcgtt taaaaatcct ggaagttctt caggagccgg acaaccatca cgtcagtgcg
    17641 gaagatttat acaaacgtct gatcgatatg ggtgaagaaa ttggtctggc tacggtatat
    17701 cgcgtactga accagtttga cgacgctggt atcgtcaccc gccacaattt tgaaggcggt
    17761 aaatccgtat ttgaactgac acagcaacat caccacgatc acctgatttg cctcgactgc
    17821 ggcaaggtta tcgaatttag tgatgattcc atcgaagcgc gtcagcgtga aattgccgca
    17881 aaacatggca ttcgcctgac taaccacagt ctctatcttt acggtcactg tgccgaaggc
    17941 gattgccgcg aagatgagca cgcgcacgaa ggcaaataag ccagcctgaa cgagaaaagc
    18001 caacctgcgg gttggctttt ttatacaagg gaaataagaa aagcgagagt tacagctctc
    18061 acttatttgt tattactgcg tatttcctgc cagaccttat cacaatcggc tttcaccgcc
    18121 tgatcatttc cggtttgcgt tgcacgcaga cactgctgat aatccagtac gcgtacactt
    18181 tcctggtcgg caaactgctg atgttgtttc tctttcttca gcacgtttaa cacgctctgg
    18241 caggcttcaa ttttttctgg cgaaccttgc gcggtgttga tacaggcgct gtatgcctct
    18301 ttcagacggc tgtcttcctg tggtgcggtt gattgcgcac aagctaccag ccccgatgcc
    18361 atcatggcga tgagaatcag ttttttcatt gaatgcggca ggcacaaaac ctgccgcgtc
    18421 gggcatcaga agatggtgaa tggtgcgatt accataaatt tcacgtcacg ctcatcctgg
    18481 aagatgttac cgtaaccgcc gccccagctt gggatgtcgg agtggttgtc gtattcggtg
    18541 aagtgcagtt tgaacatcgt gcctttggcg cgaccgtcct gaatggtgta gaccgcatcc
    18601 aggctgtagg cagactcttc aatagtccgg tttttgtcgt agtacgcatc cggattgctc
    18661 tgccaggtcg caggtttagc atcccatgcg taaacgtagg aagcgccgat ggcgaagcct
    18721 ggaagattcc agtttttcag gtcatacatc gcactgaaga agaccgcttt ttcgccgtta
    18781 gcgttgaagt cggaacggtt atcccaccag atatccaggc ggccgttgga ggaagcgtag
    18841 gttggggtca tacgttgcag gaagtatccc tgctgactgt cagccttaat ccaggtgcct
    18901 tcgaggcgca aatccactac gtcagccgcc cggtaaccaa aggtcaacgc ttgcagccag
    18961 gcggtgccgt cataaagatc gttgacgctg cgatcgtcaa ctttatcgcg ggtaccatag
    19021 aactggtagc tggtggttaa cgggctaccg gcgatatcaa atttgtagct ggctttggca
    19081 aaatattgat cgatataccc ttccgcctga ccaaatgccg cttccagtac gaagttattt
    19141 ttgaagtcgt atttcgcacc gatggagtgc agataatcaa ctttggtggt tttatcgttc
    19201 tgataaaact catccatttc cagatgccac ggcgctttgt attcgttggt ccacatgtag
    19261 gagaaactca aggcaccagc atcgccgtaa tcaaaattcg ccccggcttc cgccccctga
    19321 taagtacccg gcataaagct ccagtgcggc gctaacagcg tttgaccggt tggctgaatg
    19381 taacctgccc tcgcccaaac cggaccgtat ttaaatttgg ccgcagcttt atacaggctt
    19441 ataccgcttt tgtcgccgga ccagtcttcg tcataggctt tattactttt tgaaaacgcg
    19501 atttcgttcg ggtgggagct gtcgccgttt tccgccattt caatcgccgt aaacgcagca
    19561 atatcaaggc cgaacatatc agcagcataa ccggactgaa aatcgaggtt ggcattccag
    19621 gtggagtgag aaaggttggt tttgtatttg tcgccgtcgg taacatcttt acggtcgcgt
    19681 tcacgctgcc agtaatagat accgccggtt aaggttgaat cgtcgatgaa tccttcggcg
    19741 cgagcctccg gcattgccat aaagcccgac attgctgtaa caccggcaat agccagcgcc
    19801 agcgtactac gtttgccact aaacgtacgc atgggttaat cctctttgac gtataaattg
    19861 ctgcaccaaa ggtgaacagc gaaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt
    19921 ctcgctgact acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa
    19981 ccagaatgta agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa
    20041 gtttgcggtt gaaaggcttg cagaagaata aaaacgtgaa attccagcaa gatgaaagcg
    20101 gttacatttt atttaattat gttttgtgat tttaaattta ttttatggat aaattgcgaa
    20161 gcggaattaa ttcgcgaaga aaagcagaaa aaacgccgca gagcgcggcg tttgggattg
    20221 tttgctattt cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcacgcaaa
    20281 ttcaatatat tgcagagatc atgtaggcct gataagcgaa gcgcatcagg caatttagcg
    20341 tttgaaattt attgtctatt tattgccgga tgcgacgtaa atgcctcacc cggcttggta
    20401 gaacttaaaa ttactcgcct actttcgccc aggtatcacg cagcccaacg gtgcggttaa
    20461 ataccggttt ttcggccgtg gaatggcggc tgtcgaggca gaagtaacct tcacgctcaa
    20521 actggaatgc tttacccgca accgcatctt tcagcgacgg ttcagcaaag ccctgtttga
    20581 tcaccagcga ttccgggtta atcaccgaca ggaaatcatc cgcagcacct gggttaggca
    20641 cgctgaacag acgatcatac aaacggattt caaccggcag cgcatgtgcc gcgctcaccc
    20701 agtgaataac acctttgact ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt
    20761 cataagtaca gaagatggtg gtgatattac cttcggcatc tttctccacg cgttcagcct
    20821 taatgacgta agcattacgc agacgcactt ctttacccag caccagacgt ttgtactgct
    20881 tgttagcttc ttcgcggaaa tcggcgcgat cgatccaaat ttcaccgcta aacggcacct
    20941 gacggctccc catttccggt ttgttcggat ggttcggcat ggtgaccatt tcgccttcgc
    21001 cctgatagtt ttcgataacc agtttcaccg gatcgataac cgccattgcg cgcggcgcat
    21061 tttcgttgag atcttcacgg atgcaggatt ccagcgacgc catctcaatg gtgttgtcct
    21121 gcttggtcac gccgatgcgt ttgcagaact cacgaataga agccgcagtg taaccacgac
    21181 gacgcagacc ggaaatggtc ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg
    21241 tcaccagcag gttcagctta cgcttggaca tcacagtgta ttccagattc aggcgcgaga
    21301 attcgtactg gcgcggatga acaggaatgg tgatgttatc cagtacccag tcgtacagac
    21361 gacggttgtc ctggaactca agcgtacaca gagagtgcgt aataccttcc agcgcatcgc
    21421 tgatgcaatg ggtgaagtcg tacatcgggt agatgcacca cttgttgcca gtctggtggt
    21481 gttcagcaaa tttaatgcgg tacagcaccg gatcgcgcat tacgataaat ggcgacgcca
    21541 tgtcgatttt cgcacgcagg caggctttac cttcttcaaa accaccggca cgcatttttt
    21601 cgaacagcgc caagttctct tcaacgctgc ggtcgcggta cgggctgttt ttacccggct
    21661 gcgtcagggt accgcggtat tcgcggatct gttccggtgt cagttcatca acgtacgcca
    21721 ggcctttatt gatcagttcg atcgcatagg cgtggagctg atcaaaataa tcggaggagt
    21781 aacggacgtt accagaccag tgaaaaccta accactctac gtcgtttttg atcgaatcga
    21841 catactcgat atcttctttt accggattag tgtcgtcgaa acgcaggttg cactggcctt
    21901 tatagtcctg ggcgatcccg aagttcaggc agatagattt cgcatggcca atatgcagat
    21961 agccattcgg ctccggcggg aaacgggtgt gtactgtgat gtgcttacca ctggccagat
    22021 cttcatcgat gatctgacgg ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg
    22081 attcctcaaa gcgtaaacaa cgtataacgg cgtatgatct tataagcggg acaggctgac
    22141 aactgttagt tttttaaaat tgataatcta acaaggatta tggggatgat tgatgaaaaa
    22201 aaaagcggaa gagaagatct ccaccgcttg tcattgttgg atgcgacgct gccgtgtcgc
    22261 atcaggcata aagcagatta ctttttgatt tcatacagcg gtgtttgacc cgccacaaca
    22321 tggccctgag ctttaatgag caagccactg aaatcgtcga tattgctgca aaccaccggg
    22381 ctaatcatcg agcgggcgtt agcgttcagg taatccagat ccatttccag aatcggttgc
    22441 cctgcgctta cctgcgcacc ctcttccacc agacgtttaa agcctttacc ttccagcgct
    22501 acggtgtcga tacccatatg gacgacgatc tccgcgcctt tttcggtttc caggcagaac
    22561 gcgtggttgg tgttgaagat tttcacgatt gtccctgcgg ctggtgatac gacgatttta
    22621 tctgtcggtt tcaccgccac accgtcaccc accgctttgc tggcgaatgc ttcgtcagga
    22681 acctgatcca gtgccacgac atcaccggta atcggcgata ccagctccgc gatagatacc
    22741 gcgtttggta cagcctgcgg ttttgctaca ggcgcggcag ttgccggagt tgcttcagct
    22801 gacgcagcgg ctaccggacc acgggcaacg actttcttca tcgcatcgcc gatggattct
    22861 gctttcgcgc caacaatcac ctgaatagtc tgtttgttca gtttcactac accagaagca
    22921 cccagacgtt tacacatcgt atcgttgacg cgggcagagt cagccacggt caggcgcaga
    22981 cgggtaatac aggcatcaat cgctttcagg ttgtcagtac cgccaaccgc agcaatatag
    23041 ttggttgcca gttgagtcag accttcttca gtattgctgt tggcttcttc agtaacgatc
    23101 tcgtcttctt tatcttcacg acccggcgtt ttcaggttga acatgcggat aaccaaactg
    23161 aacaccacga agtagatagc gaagaagata acgcccatca ccagcagcat ccagacgttc
    23221 tggctggcgg caggcaggtt atacatcaac gcgtagtcga tagcacccgc agagaaagag
    23281 aagccggcat ggatacccag cagcgttgcc acaaacaggc tgataccggt cagcagtgcg
    23341 tgcaggaggt acagcagcgg cgcaaggaac atgaacagga attccagcgg ctcagtcaca
    23401 ccggtcagga acgcagtaac agcaacagaa agcagcatcc cgccaaccat cggacgacgc
    23461 tctttcggtg ctgcgaagta catcgccagc gccgcacccg gcagaccgaa catcatgatc
    23521 gggaagaagc cggacatgaa catccccgcg gtgccgtcac cagcgtagaa acggttgatg
    23581 tcgccgtgga aaaccgtacc cgccgcgttg gtgaattcac caatctggaa ccaggcgatg
    23641 gtgttcagca cctgatgcag accggttggg atcagcagac ggttgatgaa accaaagata
    23701 ccggaaccca gcgcgcccgc agaaacgatc cactcgccgc ctgcatggat agcgtgctgt
    23761 accggcggcc agacgtaacc aaaaatggcc gccagcacca gacagaagaa gccggtggcg
    23821 atcggcacaa agcgtttgcc gccgaagaag ctcaggaagt ccggcagttt aatatcggac
    23881 caacggttat aggctgcgcc accaaccaga ccggtaatga tacccgccag tacacccatg
    23941 ttaatttctg ggttgatggt caccatcgct ttggttaaca caaagtaacc taccgcaccc
    24001 gccagcgccg ccgcacctgc gctgtctttc gaccagctgg atgccacacc gatggcgaag
    24061 attaatgcga ggttatcaaa aattgcaccg cccgcctggg caataaacgc aacgttaagt
    24121 aaatctggct gaccgaatcg cagcaacagc gccgccaccg gcagcaccgc gatagggagc
    24181 tgtaacgccc taccgagtcg ctggaaaaaa cctaaaatat tcatcttatt ccccctacga
    24241 gaaccctatt tggctcgttt aaagccgtat ttttattttg ctgcaaattt tactgccgat
    24301 gttctgtaat cagattgtta gatcatctgc tacagagtgt gtgaaaattt aattcgcatc
    24361 gcaaattaaa cgcgtgtctt ttgtgagttt tgtcaccaaa tatcgttatc atcactccct
    24421 tttactggct aaaccagaaa acttatttta tcattcaaaa aatcaagtcg gattgacgcc
    24481 tgtctgcgca aatccaggtt acgcttaaag atgtctaatc cgccaacggc ttacatttta
    24541 cttattgagg tgaataatga gactgatccc cctgactacc gctgaacagg tcggcaaatg
    24601 ggctgctcgc catatcgtca atcgtatcaa tgcgttcaaa ccaactgccg atcgtccgtt
    24661 tgtactgggc ctgccgactg gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat
    24721 gcataaagca ggccaggtca gctttaagca cgttgtcacc ttcaacatgg acgaatatgt
    24781 cggtctgccg aaagagcatc cggaaagcta ctacagcttt atgcaccgta atttcttcga
    24841 tcacgttgat attccagcag aaaacatcaa ccttctcaac ggcaatgccc cggatatcga
    24901 tgccgagtgc cgccagtatg aagaaaaaat ccgttcttac ggaaaaattc atctgtttat
    24961 gggcggtgta ggtaacgacg gtcatattgc atttaacgaa ccggcgtctt ctctggcttc
    25021 tcgtactcgt atcaaaaccc tgactcatga cactcgcgtc gcgaactctc gtttctttga
    25081 taacgatgtc aatcaggtgc caaaatatgc cctgactgtc ggtgttggta cactgctgga
    25141 tgccgaagaa gtgatgatta tggtgctggg tagccagaaa gcactggcgc tgcaggccgc
    25201 cgttgaaggt tgcgtgaacc atatgtggac catcagctgt ctgcaactgc atccgaaagc
    25261 gatcatggtg tgcgatgaac cttccaccat ggagctgaaa gttaagactt taagatattt
    25321 caatgaatta gaagcagaaa atatcaaagg tctgtaattg ttatccctgc cctggttcct
    25381 tgctcagggc aatatttttt aaaatcgggg ggcagaatgt atgcattaac ccagggccgg
    25441 atctttaccg gccacgaatt tcttgatgac cacgcggttg ttatcgctga tggcctgatt
    25501 aaaagcgtct gtccggtagc ggaactgccg ccagagatcg aacaacgttc actgaacggg
    25561 gccattctct cccccggttt tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt
    25621 aacgacaccg ctgaagcggt cagcgtggaa acgctggaaa tcatgcagaa agccaatgag
    25681 aaatcaggct gtactaacta tctgccgacg cttatcacca ccagcgatga gctgatgaaa
    25741 cagggcgtgc gcgttatgcg tgagtacctg gcaaaacatc cgaatcaggc gttaggtctg
    25801 catctggaag gtccgtggct gaatctggta aaaaaaggca cccataatcc gaattttgtg
    25861 cgtaagcctg atgccgcgct ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa
    25921 gtgaccctgg caccggaaat ggttcctgcg gaagtcatca gcaaactggc aaatgccggg
    25981 attgtggttt ctgccggtca ctccaacgcg acgttgaaag aagcaaaagc cggtttccgc
    26041 gcggggatta cctttgccac ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa
    26101 ccgggcctgg cgggcgcgat cctcgacgaa gctgacattt attgcggtat tatcgctgat
    26161 ggcctgcatg ttgattacgc caacattcgt aacgctaaac gccttaaagg cgacaaactg
    26221 tgtctggtta ccgacgccac cgcgccagca ggtgccaaca ttgaacagtt catttttgcg
    26281 ggtaaaacaa tatactaccg taacggactt tgtgtggatg agaacggtac gttaagcggt
    26341 tcatccttaa ccatgattga aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg
    26401 gatgaagtgc tgcgtatggc gacgctctat ccggcgcgtg cgattgacgt tgaaaaacgt
    26461 ctcggcacgc tcgccgcagg taaagtagcc aacctgaccg cattcacacc tgattttaaa
    26521 atcaccaaga ccatcgttaa cggtaacgag gtcgtaactc aataagagaa agtatgacac
    26581 caggcggaca agctcagata ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg
    26641 tttatcgcct gattgaccag tacgggccaa tctcgcggat tcagattgcc gagcaaagcc
    26701 agcttgcccc cgccagcgta accaaaatta cgcgtcagct tatcgaacgc gggctgatca
    26761 aagaagttga tcagcaggcc tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa
    26821 cccgcaattt ccacgcaatc ggcgtacggc ttggtcgtca tgacgccacc atcactctgt
    26881 ttgatctcag cagcaaagtg ctggcagaag aacattaccc gctgccggaa cgtacccagc
    26941 agacgctgga acatgccctg ctgaatgcca ttgctcagtt tattgatagc taccagcgca
    27001 agctgcgcga gctaatcgcc atttcggtga tcctgccagg gcttgttgac ccggacagcg
    27061 gcaaaattca ttacatgccg catattcagg tagaaaactg ggggctggta gaagcactgg
    27121 aagaacgttt taaagtgacc tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg
    27181 agcactactt cggtgcaagt caggattgcg aagactccat tctggtgcgt gtccatcgcg
    27241 gaaccggggc cgggattatc tctaacgggc gcatttttat tggccgcaac ggtaacgtcg
    27301 gtgaaattgg ccatattcag gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg
    27361 gctgcctgga aactatcgcc gccaacgctg ccattgaaca acgggtgttg aatctgttaa
    27421 agcagggcta ccagagccgt gtgccactgg acgactgcac catcaaaact atctgcaaag
    27481 ccgcgaacaa aggcgatagc ctggcctcgg aagtgattga gtatgtcggt cgtcatctgg
    27541 gtaaaaccat cgccattgct atcaacttat ttaatccgca aaaaattgtt attgctggtg
    27601 aaatcaccga agccgataaa gtgctgctcc ctgctattga aagctgcatt aatacccaag
    27661 cgctgaaggc gtttcgtact aatctgccgg tggtacgttc tgagctggat caccgctcgg
    27721 caatcggcgc ttttgcgctg gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt
    27781 tgctggaaaa ttaatgtgct tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc
    27841 catgaccatt aaaaatgtaa tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc
    27901 cgtaccgggt gcagcggaat ttttgcacgg gattatggat aaaggcctgc cgctggtgtt
    27961 gctgaccaac tatccttcgc agactgggca agatctggcg aaccgctttg ccactgccgg
    28021 tgtcgatgtg ccggatagcg tgttttatac ctctgcgatg gcgaccgccg atttcctgcg
    28081 tcgctaggaa ggcaagaaag cgtatgtggt gggcgaaggc gcactgattc atgaactgta
    28141 caaagccggt ttcactatta ccgatgtgaa ccctgatttc gtgattgttg gcgaaacacg
    28201 ttcctacaac tgggacatga ttcataaagc agcctatttc gtcgctaacg gtgcacgctt
    28261 tatcgccacc aacccggaca cccacgggcg cggtttttat cccgcttgtg gcgcgttgtg
    28321 tgcagggatt gagaaaatct ccgggcgcaa accgttctat gttggtaagc ccagcccgtg
    28381 gatcatccgc gcggcattaa acaaaatgca ggcgcattcg gaagaaacag tgattgtcgg
    28441 cgataacctg cgtaccgata ttctggcggg cttccaggcc ggtctggaga cgattctggt
    28501 gctttctggt gtttcatcgc tcgacgatat cgacagtatg ccgttccgcc caagctggat
    28561 ttacccgtca gtcgctgaaa tcgacgttat ctgaatacca cccacgtctg cggacgtggt
    28621 tggctctgat gacaaacgcc aaactgcctg atgcgctacg cttatcaggc ctacgcagct
    28681 cctgcaatat attgaatttt catgcttttg taggccggat aaggcgttca cgccgcatcc
    28741 ggcatgaaca aatcgcactt tatcagcaat ttggcccccg taaaatataa ccattcaata
    28801 aaacccgcta aaaattatcg attcatcaat aaatacatcc tcacaccaga ccctttttca
    28861 ccaatcgaca atcaccatta cgttttttat ttttgcactg ccaaatagct tgcgcacagt
    28921 gctgctttgc ggcatttttt taaacaagca aacacaacaa gcaacaaata ccaggttaac
    28981 ggagaaggtt atgtgttcaa tttttggcgt attcgatatc aaaacagacg cagttgagct
    29041 gcgtaagaaa gcactcgagc tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg
    29101 tatttatgcc agcgataacg ccattctcgc tcacgaacgt ctgtcaattg ttgacgttaa
    29161 cgcgggggcg caacctctct acaaccaaca aaaaaaccac gtactggcgg taaacggtga
    29221 aatctacaac caccaggcat tgcgcgctga atatggcgat cgttatcagt tccagacagg
    29281 gtctgactgt gaagtgatcc tcgcgctgta tcaggaaaaa gggccggaat ttctcgacga
    29341 cttgcagggc atgtttgcct ttgccctgta cgacagcgaa aaagatgcct acctgattgg
    29401 tcgcgaccat ctggggatca tcccactgta tatggggtat gacgaacacg gtcagctgta
    29461 tgtggcctca gaaatgaaat cgctggtgcc agtttgccgc acgattaaag agttcccggc
    29521 ggggagctat ttgtggagtc aggacggcga aatccgttct tattatcatc gcgactggtt
    29581 cgactttgat gcagtgaaag ataacgtgac cgacaaaaac gagctgcgtc aggcactgga
    29641 agattccgtt aaaagccatc tgatgtctga tgtgccttac ggtgtgctac tttctggtgg
    29701 tctggattcc tcaattattt ccgctatcac caagaaatac gcagcccgtc gcgtggaaga
    29761 tcaggaacgc tctgaagcct ggtggccgca gttacactcc tttgctgtag gtctgccggg
    29821 ttcaccggat cttaaagcag cccaggaagt ggcaaaccat ctgggcacgg tgcatcacga
    29881 aattcacttc actgtacagg aaggtctgga tgccatccgc gacgtgattt accacatcga
    29941 aacttatgat gtgaccacta ttcgcgcttc aacaccgatg tatttaatgt cgcgtaagat
    30001 caaggcgatg ggcattaaaa tggtgctgtc cggtgaaggt tctgatgaag tgtttggcgg
    30061 ttatctttac ttccataaag cgcccaacgt taaagaactg catgaagaga cggtgcgtaa
    30121 actgctggcc ttgcatatgt atgactgcgc gcgcgccaac aaagcgatgt cagcctgggg
    30181 cgtggaagca cgcgttccgt tcctcgacaa aaaattcctt gatgtggcga tgcgcattaa
    30241 cccgcaggat aaaatgtgcg gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt
    30301 tgagtcatac ctgcccgcaa gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt
    30361 cggttacagt tggatcgaca ccctgaaaga agtggcggcg cagcaggttt ctgatcagca
    30421 actggaaact gcccgcttcc gcttcccgta caacacgccg acctcaaaag aagcgtatct
    30481 gtaccgcgag atctttgaag aactgttccc acttccgagt gccgctgagt gcgtgccggg
    30541 cggtccttcc gtcgcgtgtt cttcagctaa agcgatcgaa tgggatgaag cgttcaaaaa
    30601 aatggacgat ccgtctggtc gtgcggttgg cgttcaccag tcggcgtata agtaagaaat
    30661 gcggtactgc cccggagaaa tccggggctg ttttagttat tgcgctgctt gttgattctg
    30721 atatgcagta aggaaatgtt ccgggtttgc cacatcactg tttgacaact catcactgta
    30781 ggccagtatt tcatagatta atttcccacc actattgttg aattccgaat ttcatcctgt
    30841 acgacaaaag aatggctgta gggtggggaa aaacacaaag taaacaataa ttgacgaata
    30901 tagcgccacg ctgttcgcaa cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa
    30961 agaggttgac gctgcaaggc tctatacgca taatgcgccc cgcaacgccg ataaggtatc
    31021 gcgaaaaaaa gatggctacg tagctcagtt ggttagagca catcactcat aatgatgggg
    31081 tcacaggttc gaatcccgtc gtagccacca tcttttttgc gggagtggcg aaattggtag
    31141 acgcaccaga tttaggttct ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca
    31201 ccattcacca gaaagcgttg tacggatggg gtatcgccaa gcggtaaggc accggttttt
    31261 gataccggca ttccctggtt cgaatccagg taccccagcc atcttcttcg agtaagcggt
    31321 tcaccgcccg gttattgggg tatcgccaag cggtaaggca ccggtttttg ataccggcat
    31381 tccctggttc gaatccaggt accccagcca tcgaagaaat aatctggcta cgtagctcag
    31441 ttggttagag cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac
    31501 caaattctga atgtgtcgaa tacgttcggc aaattcaaaa accaatttgt tggggtatcg
    31561 ccaagcggta aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc
    31621 agccaattta ttcaagacgc ttacatcgta agtgcaccca gttggggtat cgccaagcgg
    31681 taaggcaccg gattctgatt ccggcattcc gaggttcgaa tcctcgtacc ccagccacat
    31741 taaaaaagct cgcttcggcg agctttttgc ttttctgcgt tcattcaatg tcgaatgcga
    31801 tgttgacacg tcttatcctt caatgtcgga tgcgacgctg ccgcgtctta tccgacctac
    31861 ggctggcacg catccggcaa tgttgtaagg ctacaaccct aacgcatatt tcagcgcctg
    31921 acgtttcaac acgccagcac gctctgccgc cattaacccg agattacgca caaaacgcag
    31981 cggcggcaga ttattactga atccggcata aaacagatcc ataccacttt gcataatgaa
    32041 gttatccgcc atgcgccgca tctggtaacg cttgaggaca ggataactgg cccacgcttc
    32101 accgtagctg cgggcattca ccagaacatc aatcagggca tcgacatcac gataaccaag
    32161 attcaccccc tgccccgcca gcggatggat ggtatgcgcg gcatcgccca ccagcgcaag
    32221 ccccggctgc acatactgca acgcatgtcg gcgcgtcagc ggaaacgcac cagcggcaag
    32281 cggtgtaacg taacccagac gcgacgggaa atgcttcgcg atttccgcct ggagttgcgc
    32341 catattcata ttctgcaact ggcgaatacg cgtcggagag tcataccaca ccagcgatgc
    32401 ccagttatca aacaacggca gaaacgcacg cggtccgtcc ggggtaaatt gctgccaggt
    32461 gctgtcgccg ggatcgttct cacactgtac gctaatcaac atgcacgact gcgcatactg
    32521 ccaggcatga acgccaattc ccgccatctg ccgcacctga gaatttgcgc cgtcggcacc
    32581 aatcaccagt ttcgcgcgga tcgtttcacc gcccttcagt tccagctcct gaagatcatt
    32641 atctcgatgt aatgcaatca gcgaggttgg cacacgtaac gttactttcg gatgcgcttc
    32701 cagcgcctgc cacaacgcct gttgcaggac tgtgttttcc accatgtagc caagcaacgg
    32761 caactttaat tcggctgcgt caaataccac atgtgccgtt tcccactccc aggtttccag
    32821 tctgcggtaa ggatggcaac gcatagcctg tactgcatcc cagaccccta accctttaag
    32881 caatgatacc gaagccgcgc tgatcgccga gatccgcacg tccggttggc tgtcagcgac
    32941 aaacggtgca ggttctgcgt gctcgatcac cgttaccgaa aatccgtgct gtgccagccc
    33001 cagcgccagt gcgccgccga ccattcctcc gccgacaatg gcaatttccg ttggttgatt
    33061 tgtcatggtc aatcatcctg ttagcaatat gctttaagtt tacaggattt ttacccccaa
    33121 gggctgataa cgctcatact ggtcacaacg gcagcaaagc attacactat gcgccctgca
    33181 ttcctggcta ctatttcgca agagcaagtc gatgaccaaa aaactccata ttaaaacctg
    33241 gggctgtcag atgaacgagt acgattcatc gaagatggcc gatctgctgg atgcctccca
    33301 cggctatcaa ctgaccgacg tggcggaaga agcggatgtg ctactgctga acacctgctc
    33361 aatccgcgag aaggctcagg aaaaagtctt ccatcagttg ggtcgctgga aactgttaaa
    33421 agagaagaat ccagacctga ttatcggcgt cggtggctgc gtggcatcgc aagaaggcga
    33481 gcacattcgc cagcgcgccc actatgtcga tattattttt gggccgcaaa cgctgcaccg
    33541 tctgccggag atgatcaact ccgtgcgcgg cgaccgcagc ccggttgtag atatcagctt
    33601 cccggaaatc gagaagtttg accgtctgcc ggaaccgcgc gccgaagggc cgaccgcgtt
    33661 tgtctccatc atggaaggct gcaataaata ttgcacctac tgcgtggtgc cttacacccg
    33721 tggtgaagag gtaagccgtc cgtctgacga tattctgttt gagattgccc agcttgcggc
    33781 tcagggcgtg cgtgaagtca acctgctcgg tcagaatgtg aatgcctggc gtggtgagaa
    33841 ctacgacggc accaccggat cgtttgccga tctgttgcgt ctggttgctg cgatcgacgg
    33901 gatcgatcgt attcgcttta ccaccagcca tccgatcgaa ttcaccgacg atatcatcga
    33961 agtgtaccgc gacacgcctg agctggtgag cttcctgcat ctgccggtac agagcggttc
    34021 cgatcgcatt ctgaacctga tgggacgtac ccatacggcg ctggagtaca aagcgatcat
    34081 ccgtaaactg cgtgcggcgc gtccggatat tcagatcagt tctgacttca ttgttggctt
    34141 ccctggcgaa accaccgaag acttcgagaa aacgatgaag ctgattgccg acgtcaattt
    34201 cgacatgagc tacagcttta tcttctctgc acgtccagga acgcctgccg ccgatatggt
    34261 tgatgatgtt ccggaagaag agaagaagca gcgtctgtat attctgcaag aacgtatcaa
    34321 tcagcaagcg atggcatgga gccgtcggat gctcggcacc acccagcgta ttctggtaga
    34381 agggacttcg cgtaagagca tcatggagct ttccgggcgt accgaaaata accgcgtggt
    34441 caacttcgag ggcacaccgg atatgatcgg taaattcgtc gatgtagaaa ttaccgacgt
    34501 ctacccgaac tctctgcgcg gtaaagtggt gcgaactgaa gatgaaatgg gtctgcgcat
    34561 ggcagaaaca ccggagtcgg tgattgcccg cacccgcaaa gaaaacgacc ttggcgtggg
    34621 ttattatcag ccgtaattct caggcctgcc gttccggtgg gccttgtttt ttctctcttt
    34681 atccccatct ttatcgcaat gcttgcgcct ttttcctgtg gcgtgaataa tttccttata
    34741 ggccggtgaa tgttcagctt ccggcggcat acagataaga ggaacggttt gaacatagac
    34801 actcgcgaaa tcaccctgga gccagcagac aatgcgcgtc tgttgagcct gtgcggcccg
    34861 tttgatgaca acatcaagca gctcgaacgc cgtctcggca tcgagatcaa tcgccgcgat
    34921 aaccacttta aactgaccgg ccgtccgatt tgcgtcaccg ctgcggcaga cattctgcgt
    34981 agcctgtatg tcgatactgc cccgatgcgc ggtcagattc aggatatcga accggaacag
    35041 atccaccttg cgattaaaga agcgcgggta ctggagcaaa gcgcggagag cgtgccggag
    35101 tacggcaaag cggtcaatat caaaactaaa cgcggcgtaa ttaagccgcg cacgccaaac
    35161 caggcgcagt acatcgccaa tattctcgac catgacatta ccttcggcgt tggcccggcg
    35221 ggtacgggta aaacctacct ggcagtggct gcggcagttg atgccctgga gcgtcaggaa
    35281 attcgccgta ttctgctgac tcgtccggcg gtcgaagccg gtgagaaact gggcttcctg
    35341 cctggcgatt taagccagaa agtagacccg tatctgcgcc cactgtacga cgcgctgttt
    35401 gaaatgctgg gctttgagaa agtcgagaaa ctgattgagc gcaacgttat tgaagtcgcg
    35461 ccgctggcct atatgcgtgg tcgtacgctg aacgacgcgt ttatcattct cgatgagagc
    35521 cagaacacta ccatcgaaca gatgaagatg ttcctgaccc gtatcggttt taactcaaaa
    35581 gcggttatca ccggcgacgt cacacagatc gacctgccgc gtaatactaa atcaggctta
    35641 cgtcatgcca ttgaagtgct ggccgatgtc gaagagatca gctttaactt cttccacagc
    35701 gaagacgtgg ttcgtcaccc ggtggtggcg cgtatcgtta acgcttatga agcctgggaa
    35761 gaagcagaac aaaaacgtaa agcggcgctg gcagcagaac gcaagcgcga agaacaggaa
    35821 caaaaatgag tcaggtgatc ctcgatttac aactggcatg tgaagataat tccgggttac
    35881 cggaagagag ccagtttcaa acatggctga atgcggtgat cccgcagttt caggaagagt
    35941 cggaagtgac tattcgcgtg gtcgataccg ccgaaagcca cagtctgaat ctgacctatc
    36001 gcggtaagga taagccgacc aacgtgctct ccttcccgtt tgaagtgccg cctggcatgg
    36061 agatgtcgct actgggcgat ctggttatct gccgtcaggt ggttgagaag gaagctcagg
    36121 agcaaggcaa accactggag gcgcactggg cgcatatggt ggtgcacggc agtctgcatt
    36181 tgttaggtta cgatcacatt gaagatgacg aagcagaaga aatggaagcc ctcgaaacag
    36241 agattatgct tgctctgggc tatgaggatc cgtacattgc cgagaaagaa taatttgcca
    36301 ggcatttgac tggcagacgt ccccgccgtt ggtgattaac gacggcgttt tgattaacta
    36361 acatgagaac ccatacgacg ccatgagcga cgacaattca cacagtagtg acacgataag
    36421 caacaagaag ggatttttct ccctgttact cagccaactt ttccacggtg aaccgaaaaa
    36481 ccgtgacgaa ctgctggcgc tgatccgtga ttccgggcag aacgacctta tcgacgaaga
    36541 tacgcgcgat atgctcgaag gggtgatgga catcgcagac caacgcgttc gcgacatcat
    36601 gatcccccgc tcccagatga ttaccctgaa acgcaaccag acgctggacg aatgccttga
    36661 tgtcatcatc gagtccgccc actcacgttt cccggtgatt agcgaagaca aagatcacat
    36721 tgaagggatt ctgatggcga aagacttgct gccgtttatg cgcagcgatg ctgaagcctt
    36781 cagcatggac aaagtgttac gtcaggcggt tgtcgttcct gaaagtaagc gcgtagaccg
    36841 gatgctgaaa gagtttcgct ctcagcgtta ccacatggcg atcgttattg acgaattcgg
    36901 tggggtttcc ggtctggtga ccattgaaga catcctggaa ctgattgttg gtgagattga
    36961 agacgagtat gacgaagaag atgatatcga cttccgtcag ctgagtcgtc atacctggac
    37021 cgtgcgcgca ctggcttcca ttgaagactt caacgaagcg ttcggcaccc actttagcga
    37081 tgaagaagtc gacactatcg gtggtctggt gatgcaggcg tttgggcatc ttccggcgcg
    37141 tggcgaaact atcgacatcg acggttacca gttcaaagtg gcgatggccg atagtcggcg
    37201 tattattcag gttcatgtca aaatcccgga tgactcaccc cagccaaagc tggatgaata
    37261 aaaccgaaac tggatagata actacatgga ttttgcctca ttaattgaac gccagcgcat
    37321 tcgcctgctg ctggcgttat tattcggtgc ctgcggaacg ctggccttct ctccttacga
    37381 cgtctggcct gcggcgatta tttcgctgat ggggcttcag gcgttgacct ttaaccgtcg
    37441 cccactccag tctgccgcta ttggcttttg ctggggattt ggcctctttg gcagcggtat
    37501 taactgggtc tatgtcagca tcgcgacctt tggcgggatg cctggcccgg ttaacatctt
    37561 cctggtggtg ctgctggcgg cgtatttgtc gctgtatacc ggactgtttg ctggcgtgct
    37621 gtcgcgtctg tggccgaaaa ccacctggct gcgcgtggcg attgccaccc ctgctctctg
    37681 gcaagtgacc gagtttctgc gcggttgggt gctgactggc ttcccgtggt tacagttcgg
    37741 ctatagccag attgatggcc cgttaaaagg gctggcaccg ctaatgggcg tggaagccat
    37801 taacttcctg ctgatgatgg ttagtggcct gctggcactg gcgttggtca aacgcaactg
    37861 gcgtccgctg gtggtggccg tcgtgctgtt tgccctaccc ttcccgctgc gttacatcca
    37921 atggtttacc ccgcaaccgg agaaaaccat tcaggtttcg atggttcagg gcgatattcc
    37981 gcaatcgctg aaatgggacg aaggccagct tcttaatacg ctaaagattt actacaacgc
    38041 aacggcaccg ctgatgggca aatcatcgtt gattatctgg ccggagtcgg cgataaccga
    38101 tctggaaatt aatcagcaac cgttcctcaa agcactggac ggtgagttgc gtgataaagg
    38161 tagctcgctg gtaaccggga ttgtcgacgc gcgtctcaat aagcagaacc gctacgatac
    38221 ctacaacacc atcatcacgc tgggtaaagg tgcaccgtac agctacgaat cagccgatcg
    38281 ctataacaaa aaccatctgg tgccgtttgg cgagtttgtc ccgctggagt cgattctgcg
    38341 tccgttagca ccgttctttg atctgccgat gtcgtcgttc agccgtgggc catatatcca
    38401 gccgccgctg tcggcaaatg gtattgagct tactgcggct atttgctacg agattattct
    38461 cgccgagcaa gtgcgcgata acttccgccc ggataccgac tacctgctga ctatctccaa
    38521 cgatgcgtgg tttggtaaat ctattggtcc atggcaacac ttccagatgg cgcgaatgcg
    38581 tgcgctggag ctggcgcgcc cactgttgcg cagcaccaac aacggcatta cggcggtgat
    38641 tggcccgcag ggtgagattc aggcgatgat cccgcagttc gcccgcgagg tgttaaccac
    38701 taacgtgacg ccgaccaccg gactcacacc atacgcacgt accggcaact ggccgctgtg
    38761 ggtgctgacg gcactgtttg gttttgctgc tgtgttgatg agtctgcgtc agcgacgtaa
    38821 ataatccctt cttatcgtgc cggaatacat cccatatttc ggcacgctca ccggatgcgg
    38881 catcagtgca ttatccggat aacaatattc tcctatccaa ctttggcaca tctattgctt
    38941 tgttatacaa ggcaaagctt gaaccagcat cagtacaacc cggttgcacc acagcaatcc
    39001 accggtagca ccaaaagagt gcattttaag aattttgctt cagaatggtg cggtgcacct
    39061 cgcaaaaaca aacaataaaa catcaatatc tttacattta gccaaactaa atgttaccta
    39121 acaggcacaa cactgcacaa taaagttgca gacgataaca acacaaacac tcacaacggg
    39181 tatccatgcg ttcttaacgc agaagataaa ggtgttggat atgcaattac gtaaacctgc
    39241 cacagcaatc ctcgccctgg cgctttccgc aggactggca caggcagatg acgccgcccc
    39301 ggcagcaggc agtacgctgg acaaaatcgc caaaaacggt gtgattgtcg tcggtcaccg
    39361 tgaatcttca gtacctttct cttattacga caatcagcaa aaagtggtgg gttactcgca
    39421 ggattactcc aacgccattg ttgaagcagt gaaaaagaaa ctcaacaaac cggacttgca
    39481 ggtaaaactg attccgatta cctcacaaaa tcgtattcca ctgctgcaaa acggcacttt
    39541 cgattttgaa tgtggttcta ccaccaacaa cgtcgaacgc caaaaacagg cggctttctc
    39601 tgacactatt ttcgtggtcg gtacgcgcct gttgaccaaa aagggcggcg atatcaaaga
    39661 ttttgccgac ctgaaaggca aagccgtagt cgtcacttcc ggcactacct ctgaagtttt
    39721 gctcaacaaa ctgaatgaag agcaaaaaat gaatatgcgc atcatcagcg ccaaagatca
    39781 cggtgactct ttccgcaccc tggaaagcgg tcgtgccgtt gcctttatga tggatgacgc
    39841 tctgctggcc ggtgaacgtg cgaaagcgaa gaaaccagac aactgggata tcgtcggcaa
    39901 gccgcagtct caggaagcct acggttgcat gttgcgtaaa gacgatccgc agttcaaaaa
    39961 gctgatggat gacactatcg ctcaggtgca gacctccggt gaagcggaaa aatggtttga
    40021 taaatggttc aaaaatccaa ttccgccgaa aaacctgaac atgaatttcg aactgtcaga
    40081 cgaaatgaaa gcactgttca aagaaccgaa tgacaaggca ctgaactaat tacaagaacc
    40141 aggggcggaa attccagccc tctcgattgt tacgtagcac ggacagacta tacgcctgat
    40201 ggtcgttccc catcgggcct gaaaaccgca atacgctggg taacaatctt cgagggtagc
    40261 agttaacgct gctaccctct tttttctgga gtagatttat gtctatagac tggaactggg
    40321 gtattttttt acaacaagcc ccgttcggca ataccaccta tcttggttgg gtctggagtg
    40381 gttttcaggt gacgatcgct ttgtcgatct gcgcctggat tatcgctttc ctcgtcggtt
    40441 catttttcgg cattttacgt accgttccaa accgttttct ctccggtctg ggtacgttgt
    40501 atgtcgaact gttccgcaac gtgccgttga ttgtgcaatt ctttacctgg tatctggtga
    40561 tcccggagct gctgccggag aaaatcggca tgtggtttaa ggccgagctg gatcccaata
    40621 ttcagttttt cctttcatcc atgctctgcc tggggctgtt taccgccgcc cgtgtttgcg
    40681 aacaggtacg cgccgctatt cagtcgctac cgcgtgggca gaagaatgcc gcgctggcga
    40741 tgggcctgac gctaccgcag acttatcgat acgtactgct gcctaatgct tatcgcgtta
    40801 tcgtcccgcc gatgacttcc gagatgatga acctggtgaa aaactccgcc atcgcctcga
    40861 ccatcggtct ggtggatatg gcggcgcagg cgggtaaatt gctcgactac tcggctcacg
    40921 cctgggagtc gtttaccgcc atcacgcttg cgtatgtgtt gattaacgct tttatcatgc
    40981 tggtgatgac gctggttgaa cgtaaagttc gcctgcccgg caacatgggg ggcaaataat
    41041 gtacgagttt gactggagtt ccattgttcc ttccctgcca tatctgctcg acgggctggt
    41101 gatcaccctg aaaatcaccg tcacggcagt cgtgattggt attttgtggg gtaccatgct
    41161 ggcggtgatg cgcttgtcca gcttcgcgcc tgtttcgtgg tttgccaaag cctacgttaa
    41221 cgtattccgc tcgattcctt tagtcatggt tttgctgtgg ttttacttga ttgtgccggg
    41281 atttctgcaa aatgtgctgg gattatcgcc aaaaaatgat attcgtttaa tttcggcgat
    41341 ggtggcgttt tcgatgtttg aagcggccta ctattcagag attatccgtg ccggtattca
    41401 aagtatttcc cgtggtcagt cgagcgccgc gctggcgttg gggatgactc actggcagtc
    41461 gatgaaactg attattctgc cgcaggcttt ccgcgcgatg gtgccgctgc tgctcactca
    41521 gggcatcgta ctgttccagg atacctcgct ggtgtatgtg ttatctctgg ccgatttctt
    41581 ccgtaccgcc tcaaccattg gtgagcgtga tggtacgcag gtcgagatga tcctgtttgc
    41641 cggatttgtt tatttcgtta ttagccttag cgcgtcgttg ttggtcagct acttgaaaag
    41701 aaggacagca taatgattac cctgaaaaat gtttcaaaat ggtatggtca ctttcaggtg
    41761 ctgaccgact gctcaaccga agtgaaaaaa ggcgaagtgg tggtggtttg cggcccgtct
    41821 ggttccggca aatcaacgct gattaaaacc gtcaacggcc tcgaaccagt gcagcaaggt
    41881 gaaatcaccg tcgatggtat cgtggttaac gacaagaaaa ccgatctggc aaagctgcgc
    41941 tcccgcgtcg ggatggtatt ccagcatttc gagctgttcc ctcatctgtc gattatcgaa
    42001 aacctgaccc tggcgcaggt gaaagtgctt aaacgcgata aagcgcccgc tcgtgaaaaa
    42061 gcattgaaat tgctggagcg cgtgggactt tccgcacatg ccaataaatt tccggcgcag
    42121 ctttccggcg gtcagcagca gcgcgtggca atcgctcgcg cattgtgtat ggctcctatt
    42181 gcgatgctgt tcgacgaacc gacatcggcg ctggatccgg agatgatcaa cgaagtgctg
    42241 gacgtgatgg tggaactggc gaacgaagga atgaccatga tggtggtgac ccacgaaatg
    42301 ggctttgccc gcaaagtggc gaatcgggtg atctttatgg acgagggtaa aattgtcgaa
    42361 gactcgccga aagacgcttt cttcgatgat ccgaaatcgg accgcgcaaa agacttcctc
    42421 gcgaaaatcc tgcattaatc actctggcgc acatacccaa ttgcgcgcca tctcacgctt
    42481 tatcgtttcg cccctcgtat ctgttacatc ctggcgttac ccttgtcgca aagaagcaca
    42541 caacaaggag caacaatggc actgccaatt ctgttagatt gcgacccagg tcatgatgac
    42601 gctatcgcaa tagttctcgc actcgcctca ccagagcttg atgtcaaagc aattacgtct
    42661 tccgccggaa accagacacc agaaaaaacc ttacgcaatg ttctgcgtat gctgaccttg
    42721 cttaatcgca ccgatattcc ggtggcaggc ggcgcggtaa aaccgttaat gcgtgagttg
    42781 attatcgcgg acaatgtgca cggcgaaagc ggtctcgacg gcccggcatt accggaaccg
    42841 acgttcgcac cgcaaaactg tacggcggtg gagctgatgg cgaaaacgct gcgcgaaagt
    42901 gcggaacctg tcacgattgt ttctaccgga ccgcaaacta acgttgcctt gctgctcaat
    42961 agccatccgg aactgcatag caaaattgcc cgtatcgtga ttatgggcgg cgcaatgggg
    43021 cttggtaact ggacgcctgc ggctgaattt aatatttacg ttgacccgga agcggcagaa
    43081 attgtcttcc agtcagggat cccggtggtg atggccggtc tggatgttac tcataaagcg
    43141 caaatccacg ttgaagacac cgagcgtttc cgcgcgattg gcaaccctgt ttcaaccatt
    43201 gttgccgaac tgctggattt cttcctcgaa tatcataaag acgaaaaatg gggctttgtc
    43261 ggcgcaccgc tgcatgaccc atgcaccatc gcctggctgt tgaaacccga gttgtttacc
    43321 actgttgagc gctgggttgg cgtggaaaca caggggaaat atacccaggg tatgacggtt
    43381 gttgattatt attatctgac tggcaataaa ccgaatgcca ctgtaattgt cgatgttgat
    43441 cgtcagggct ttgttgattt actggctgat cgtctgaaat tttacgctta acactcgtca
    43501 ttgattaaga tcaccacgcg ttacctgggt ggtcttaatc tatttatctc tttttctttc
    43561 aagatataaa tgcaatggat aatgcagaac tcgccatagg tatcgatctc ggcactacca
    43621 atagtttaat tgccgtctgg aaagacggtg ccgcgcaatt aattccaaat aagttcggtg
    43681 aatatttaac accatccata attagcatgg atgaaaataa tcatatttta gtcggaaaac
    43741 cggctgtatc acggcgtact tcgcatccgg ataaaacggc agcgttattt aaacgtgcaa
    43801 tgggcagtaa taccaactgg cggttaggca gcgacacatt taacgcgcca gaactgtcct
    43861 ctttggtatt acgctcatta aaagaagatg ccgaagaatt tctgcaacgt ccgattaaag
    43921 atgtggtgat ctccgttctg gcttatttca gcgatgaaca acgcaagcat acccgtttag
    43981 cagcggactt agccgggtta aatgcggtac gcttaattaa tgaacccaca gcagctgcga
    44041 tggcgtatgg cctgcatacc caacaaaata cccgttcgct ggtgtttgat ctcggtggcg
    44101 gcacgtttga cgttacggtg cttgagtacg ccacgccggt gattgaagtt cacgcctccg
    44161 ctggcgacaa cttcctcggt ggcgaagatt ttacccatat gttggtcgat gacgttttaa
    44221 agcgcgcgga tgtcacaagg accacgctta acgagagtga actggcagcc ttgtacgcct
    44281 gtgtggaagc ggcaaaatgt agcaatcaat cgccaatgca cattcgctgg tagtaccagg
    44341 atgaaacgcg ggaatgcgaa ttttacgaga acgaactgga agatttgtgg ttgccgctgc
    44401 tcaatcgctt gcgagtgccg attgaacagg cgttgcgcga tgcgcgtctg aagccgagtc
    44461 aaatcgacag tctggtgctg gttggcggcg cgtcacaaat gccgctggtg cagcgaatcg
    44521 ccgtgcgtct gtttggcaaa ttaccgtatc aaagttacga tccgagcacc attgtcgcgc
    44581 tgggcgcagc aatccaggcc gcctgccgct tacgcagtga agatattgaa gaagtgatcc
    44641 tcactgatat ttgcccttac tcgttgggcg ttgaggttaa ccgccagggc gtttccggca
    44701 ttttctcgcc gattattgaa cgaaacacca ctgtgcccgt gtcgcgtgta gaaacttatt
    44761 caaccatgca cccggaacag gattcaatta cggttaacgt ctatcaggga gaaaaccaca
    44821 aagttaaaaa caacattctg gtggaatcct tcgatgtgcc gttgaagaaa accggggctt
    44881 atcagtcgat tgatattcgc tttagttatg atatcaacgg gttgcttgaa gttgacgtgc
    44941 ttctggaaga cggcagcgtt aagtccagag tgattaacca cagcccggta acattgagcg
    45001 cgcagcagat tgaagagagt cggacgcggt tatccgcatt gaaaatttat ccgcgcgata
    45061 tgctcatcaa tcgcaccttt aaagccaaac tggaagagtt gtgggcgcgg gcgctgggtg
    45121 acgagcgaga agagattggc cgggtgatca ccgattttga tgcggcgctg cagtcaaacg
    45181 atatggcccg cgtcgatgag gtcaggcggc gggcgagcgt ttatttagcc attgagacct
    45241 cataatcacg gctgccggat gcggcgtaaa tgctgcatcc ggcatcggcg caaaaaacac
    45301 atcgtcaact aaacaacatc gcgattttgc cgagagcata acctataacc agcaccgtta
    45361 aactgatttt gataatgctg gtaatgctct gtggtttttc accctcatca tttttttctt
    45421 catggttttc aggtgttggc tcatcgacat cttcatcctc cggctcgagt tttactttcg
    45481 tcaacagcat ccgggtgata atggcctcta atggatgagc atctccgcct tccagccttg
    45541 tattaagacg ggccagcatc tcttgtttcg gaaacgcttc aagtactacc tgttccgcta
    45601 cccgatacaa atccagtaac ggtgaatctt cttctgtctc gctaccgact ctcgatttcc
    45661 accacgccca tgctttgcgt ttgaagaata acgactcaat aaacgattga cctaacggcg
    45721 ataattttcc gctatgtaaa atttccgccc ataacagccg cgtccgggcg ctctcaccag
    45781 gaacatattt cagcggttga ccttgcgcat ctaccatgct ccgcgcttcg ataaccatca
    45841 ctaataacgc gaaataatcc gggcaatgac gcttgctcca ctgcaacaac aaattatcag
    45901 cttgtgtaga tgggtaactt tcccagtacg cgccgagatc agcaagcagg ctttccccct
    45961 cgccgcagta caaacgttgt gcacactggt aataccgggc atcttcgctt tctggttctt
    46021 ccgcttgcca ctgtttggct acaggaatga gttccaccat acctgtttgc accgagctgt
    46081 accagcgcaa cagtttgcga tgtaactttg catcatcagg aatgaaccac ggtccttcca
    46141 tcgccaaaaa cgcagtgaca tagtcaggat ggtaacggca gcaacgttcc agcgcgaagt
    46201 aatattcaac agtctggttc tgtaacgcga cgggatattc cagaaggcta aggaaatcga
    46261 aacaatcacc agcctgaatg gcctcaagaa aagcctccct ttcttcctca tccattcctt
    46321 cgctgtcatt aaacgaatgc cagttcaaac gctctgccaa caaagaagca caatttaatg
    46381 acagatatcg cgcttctata gcgattgcac acagcggcca gcgtaattga tcgacatcct
    46441 ccatgttcca ggtatttaat tgctgaataa atttctgcca ggcggagggc tgaaaacgat
    46501 cactttctga atccagtaat gtcctgaatg cacgtagtat ttcatgttca gcggtggcat
    46561 ctttttcttc atcatctgct tcctcgacag gatttaccgc cagtcgcagc gcctcttcat
    46621 aggcctggcg taatgcttta aacccctgcg gatcggtttc gggatgacac aacggtaagc
    46681 gagcaagata agcctggcgg ataatgtcta tttgcgtcgt gctttcaatt tcgaggattt
    46741 gccaacatgt tttcataggt cgctcacatc cttaagcgtt accgggcatt caggctggac
    46801 agttaatggc tgccagggaa gcgtggcata gaaacggtcg cttcccgctg ccagctcttc
    46861 gaacatccga ctcccggcgc tatcgtattt gtacagggtt agcgcctcgc gtagcgaagc
    46921 gttgtcttcg cactgagaag attgccagta gagacgccct agtgaatagg cagaaaaata
    46981 gtgcatccag ctgtgatagt aatggtgagc tcgtgcatag acgcgcgatt gcagccataa
    47041 actctcttct tccgtcagcc agttgttgag cacaccggcc cgtgacagaa atcccattcg
    47101 aacataatcc caggctttga ttcccccttc tccacagcac atagcggttt gggcaacgaa
    47161 ttgggtataa atcaaccctc tctccgagcc accagcaatt aatgctttcc actcttcagg
    47221 ggaagagcgt tgccatgtca gatataagcc agctaaatca ttggcatgac catcatcagt
    47281 catctgataa atagtctgaa ttaaatcatc acgattattt attccccagg atgatgagag
    47341 atcgaattct tttatcccgg tatacatttt tggcgagtca tgcgcgggtt tgcaactctg
    47401 attaagtatt tccatgggcg cagaaagcgc aaaaagaaga tattgttctt ccttattcat
    47461 tttttaataa tcccttaagt gcattaactg actacttttt tattattgat gagtataaac
    47521 ttgacgcttt caaaataaaa agaaaatcga agcattcaca catgaataaa aaattaatgt
    47581 atatattcgc aatttttata gttgcagcaa ttacctgtat tagccaaccc aagaaaacga
    47641 tgttgcgtga taaagccatg gtgaattatg cctttgatta tttaagatca ccaggcagtc
    47701 taccgttaac cacggcagcc acggagcttt ccgcgattca tggtcactca acatcgcaat
    47761 atcgccttgg cgaattttat cttcatggta gcgacggtaa accactggat tatacacagg
    47821 cgagatactg gtatgagcaa tcagcagaac aggaaaatcc gcgcgcacaa agcaaactgg
    47881 ggtggatcta cctgaaaggc ctgggagtca aacccgatac ccgtaaagca attctctggt
    47941 ataaggaagc agctgaacaa gggtatgctc atgctcaata tactttgggt ttgatctaca
    48001 gaaatggcac tggtattaat gttaaccatt atgaatctca aaaatggtta aaactggccg
    48061 ccaaacaaca ttacaaaaat gcggaaagat tacttgccgg acttcccgca cattaacgtc
    48121 gactgtcacc cgaagagatg gagtattttc cctatcaaac cggcaaagat aaaaatgtag
    48181 aaaataattt tgatgacgcc cagacagccc agcccgccat catcaacggg cattggctgc
    48241 ggttgcggct ccggctcgac ggtttcctga ccggtaaaat tcgccgcctg aattaataat
    48301 cgacgaataa ttaatgcttc cagtggacaa cgatcttctg catcaaggcg gctcacaatc
    48361 ggcattaatg attcttctgt cggttggctg gcctgcacta attgtttggc aactaaatat
    48421 aatggccata agggatgttc tgtatcacgc gtgagtgaga tccgcggctt acgtcctttg
    48481 tgcaaaacca tctcgaccag tgaacgcccc agcgcagaga gtttttcatc gctgagtatt
    48541 tcgtataaat gaaaacgcgt acgggcgctg tcgccgggga cataaagtag cggttttccc
    48601 tgatcgttaa ccagatcacg cgcttcaatc atcatcacca ctaatgggta ataatagacc
    48661 cgatgctgac ggcaccattg caacattaaa gcatccgcct gggtggaggg atactcgcgc
    48721 cagtagtaac aaagttctgc cagcaggctt tcgccttctc cacaatagac gcgttgtgcg
    48781 tattcataat aacgcgcatc ttcattatca ggttcagctt cccgccacga ccgggcgtaa
    48841 tccaggagtt cggggattcc caggcggctt gcgctatacc agcggagtaa atttctgtgc
    48901 agttttgcat catcggggat aatcactgcg cgatgttgcg ctaaatacaa tgtcagccat
    48961 tctggatgcg atgaccaaat ccgagcgaac atttggtaaa aatcgatggt gtcattttgc
    49021 acggcaactg gcagatgcag aatggtctgg aagttaaaaa cattcccctt cgcaatggca
    49081 tataaaaagg attccagttc ttcttcgtct atttccccga cgttttcctc ctgcaaccac
    49141 cgcaatcttt ctgctaataa caccacgcac tcgaaggata aatgggcagt gttcatggcg
    49201 attgtgcaca gcgaccagcg taattcatca atctcttcca tcgagcaata atttaattgc
    49261 tgaatgaatc gctgccaggc ggagggcaga aaacgttcgc tatcagaggc aagtaacgcg
    49321 cgaaaggcga gcagaatttc atgttctgct acctcatatt cttctggttg ccaaacagat
    49381 ttagcaggcg actgcgcaat ccgtagcgcc tcctcatacg cctgacgaag ttgtttaaaa
    49441 ccctgcggat cggtttctgg atgaaaggac ggtaacagcg ccagataagc gcggcggata
    49501 atatcgacgt cagtcgtttc ctctatatcg aggatcttcc agcaattttt cataagtcgc
    49561 tcatatcctt aagcgtttcc gggcatgtgg ggtagtcagc cagtggttgc caggacaagg
    49621 tagggtaata cgcatcatca ctggcaaata attgataaaa catatcattg ccggaatcgt
    49681 cgtattcttt acgcgctaag aattcgcgta ataaaggcag atgatcatct tcgggagact
    49741 gccaatattg ccgaccaaag gtataaccgg caaagtattg ccgccagtta ctataataac
    49801 gtagagcccg tagatgaata cgcgactgga tccatagact ttcttcttca ctcaaccagt
    49861 tattaagtac gcccattcga cttaaaaaac ccatccggac ataatcccat gctttaattc
    49921 ctccctcacc acagcattcg gctgtgctgg cgacaaattg cgcataagcc tgaccttgct
    49981 catttaattc agcaagatag tcacgccatt cacatggaga ataacgaaac cagcggtgat
    50041 aaaaccctgc caaccgagcg gcatgaccgt tctcgatcat tcggtgaatt atgcgcagca
    50101 ggtcatcacg gtcatcaatt ccccatgagg aagaaaggtc aaaaaagcat gtacctaaat
    50161 acattttggg ggatgaatgc gaaggtaaac agcattcatt gcgtatttcc atcggagtgg
    50221 aaagtgcaaa caatattttt tgcgacccca tgtccatata atttatcctt aaacattcat
    50281 ttaatgaatg taaactacat ttcgaggctc agttatttat acttttctga cgcaacataa
    50341 tacgcataaa ataaggctta cgcaaaaatg gaacacataa aaatataata tattttatct
    50401 acaaccataa ggaaatgata atgattttta cgtcaagttg ctgcgataat ttatcaatag
    50461 atgagattat cgaacgtgct gaaaaaggcg attgcgaggc tcaatatatt gttgggtttt
    50521 attataatcg cgatagcgca attgactctc cggacgacga aaaggccttt tactggctga
    50581 agctggccgc tgagcaaggt cattgtgaag cacagtattc cttagggcaa aagtataccg
    50641 aggataaaag ccgtcataaa gataatgagc aagccatctt ctggctgaaa aaagctgccc
    50701 tacaaggcca tactttcgct tccaacgccc ttggctggat actggatcgt ggagaagacc
    50761 ccaactataa agaagcggtt acctggtatc agatagccgc ggagagcgga atgtcttatg
    50821 cgcaaaataa tcttggatgg atgtacagaa atggcaacgg agtcgcacaa gactatacgc
    50881 tggcattttt ctggtacaaa caagctgcat tacaaggcca tagttacgca caagacaatc
    50941 tggccgatct ttataaagac ggagaaggcg ttgctcaaaa caagacactc gccgcattct
    51001 ggtatttgaa aagcgcacag cagggtaatc ggcacgccca gtttcaaatt gcatgggatt
    51061 ataacgctgg cgaaggggtg gaccaggact ataagcaagc gatgtactgg tatctgaagg
    51121 ctgccgctca ggaaagcgtc ggcgcttacg tcaacatcgg ttatatgtat aaacacggac
    51181 aaggcgttga gaaagattat caggctgcct ttgaatggtt tacgaaagcc gctgaatgca
    51241 atgacgccac tgcctggtat aacctggcca ttatgtatca ctacggagaa ggaagacctg
    51301 tcgatctccg acaggctctc gacctgtatc gtaaagttca gtcatccgga accagagatg
    51361 tcagtcagga aattcgtgag actgaagctt tactgtagaa ataaaaacaa gggccgggga
    51421 atttcattct gcccatattc tctttattgc tgtgcgaaat cagactatta cggctcaaac
    51481 gggcgtctct ggaactggtc ataaccacat ttcgggcata gcgtcagcac ttccggtgtg
    51541 tagatcggga gatggaagtg acatttctcg cagaccagat ttcccagccc gaccacttct
    51601 ccgctgtgat aaaccccatg atgattgagg tcctggaaaa tttcgcgcca ttcaagctgc
    51661 gttttatcgg tgatgtctgc cagctcctgc cacaagcttt ctttaatcac ccgcataaag
    51721 acgctgtcag attcttcttt caggctctct tcatagctca tggcgaactc ttccaggtca
    51781 cgtctgacag ctcgcgtcag ctcatcgatc tcggttcgcg ttaactcccc tgtttttatt
    51841 acgcgctcgc gcgcctgttc caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt
    51901 tcgttcagtg acgcgaccag ttcacggtaa tattgagcaa ccttgttcat cgtttcatct
    51961 cccgggtcgt taactgacta taataataga cgttctttgt tccttgcttt gctaatacgc
    52021 ccacaaactc tgtaaattaa tgtacgaata atccgggcat catttcaacg tcgagtgcgc
    52081 gaaaggctgt tttgacgcgg acgtttgggc tatgctatgc ggatctgaaa aaccacctca
    52141 acgctacatt tgtagccgta ttgaaaacag gaccactggc tgccatgcaa gagcaatacc
    52201 gcccggaaga gatagaatcc aaagtacagc ttcattggga tgagaagcgc acatttgaag
    52261 taaccgaaga cgagagcaaa gagaagtatt actgcctgtc tatgcttccc tatccttctg
    52321 gtcgactaca catgggccac gtacgtaact acaccatcgg tgacgtgatc gcccgttacc
    52381 agcgcatgct gggcaaaaac gttctgcagc cgatcggctg ggacgcgttt ggtctgcctg
    52441 cggaaggcgc ggcggtgaaa aacaacaccg ctccggcacc gtggacgtac gacaacatcg
    52501 cgtatatgaa aaaccagctc aaaatgctgg gctttggtta tgactggagc cgcgagctgg
    52561 caacctgtac gccggaatac taccgttggg aacagaaatt cttcaccgag ctgtataaaa
    52621 aaggcctggt atataaaaag acttctgcgg tcaactggtg cccgaacgac cagaccgtac
    52681 tggcgaacga acaagttatc gacggctgct gctggcgctg cgataccaaa gttgaacgta
    52741 aagagatccc gcagtggttt atcaaaatca ctgcttacgc tgacgagctg ctcaacgatc
    52801 tggataaact ggatcactgg ccagacaccg ttaaaaccat gcagcgtaac tggatcggtc
    52861 gttccgaagg cgtggagatc accttcaacg ttaacgacta tgacaacacg ctgaccgttt
    52921 acactacccg cccggacacc tttatgggtt gtacctacct ggcggtagct gcgggtcatc
    52981 cgctggcgca gaaagcggcg gaaaataatc ctgaactggc ggcctttatt gacgaatgcc
    53041 gtaacaccaa agttgccgaa gctgaaatgg cgacgatgga gaaaaaaggc gtcgatactg
    53101 gctttaaagc tgttcaccca ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg
    53161 tattgatgga gtacggcacg ggcgcagtta tggcggttcc gggtcacgac cagcgcgact
    53221 acgagtttgc ctctaaatac ggcctgaaca tcaaaccggt tatcctggcg gctgacggct
    53281 ctgagccaga tctttctcag caagccctga ctgaaaaagg cgtgctgttc aactctggcg
    53341 agttcaacgg tcttgaccat gaagcggcct tcaacgccat cgccgataaa ctgactgcga
    53401 tgggcgttgg cgagcgtaaa gtgaactacc gcctgcgcga ctggggtgtt tctcgccagc
    53461 gttactgggg cgcgccgatt ccgatggtga cgctggaaga cggtaccgta atgccaaccc
    53521 cggacgacca gctgccggtg atcctgccgg aagatgtggt gatggacggc attaccagcc
    53581 cgattaaagc agatccggag tgggcaaaaa ctaccgttaa cggtatgcca gcgctgcgtg
    53641 aaaccgacac tttcgacacc tttatggagt cctcctggta ctatgcgcgc tacacttgcc
    53701 cagagtacaa agaaggtatg ctggattccg aagcggctaa ctactggctg ccggtggata
    53761 tctacattgg tggtatcgaa cacgccatta tgcacctgct ctacttccgc ttcttccaca
    53821 aactgatgcg tgatgcaggc atggtgaact ctgacgaacc agcgaaacag ttgctgtgtc
    53881 agggtatggt gctggcagat gccttctact atgttggcga aaacggcgaa cgtaactggg
    53941 tttccccggt tgatgctatc gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag
    54001 atgcggcagg ccatgaactg gtttataccg gcatgagcaa aatgtccaag tcgaagaaca
    54061 acggtatcga cccgcaggtg atggttgaac gttacggcgc ggacaccgtt cgtctgttta
    54121 tgatgtttgc ttctccggct gatatgactc tcgaatggca ggaatccggt gtggaagggg
    54181 ctaaccgctt cctgaaacgt gtctggaaac tggtgtacga gcacacagca aaaggtgatg
    54241 ttgcggcact gaacgttgat gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc
    54301 ataaaactat cgctaaagtg accgatgata tcggccgtcg tcagaccttc aacaccgcaa
    54361 ttgcggcgat tatggagctg atgaacaaac tggcgaaagc accgaccgat ggcgagcagg
    54421 atcgcgctct gatgcaggaa gcactgcagg ccgttgtccg tatgctcaac ccgttcaccc
    54481 cgcacatctg cttcacgctg tggcaggaac tcaaaggcga aggcgatatc gacaacgcgc
    54541 cgtggccggt tgctgacgaa aaagcgatgg tggaagactc cacgctggtc gtggtgcagg
    54601 ttaacggtaa agtccgtgcc aaaatcaccg tcccggtgga cgcaacggaa gaacaggttc
    54661 gcgaacgtgc tggccaggaa catctggtag caaaatatct tgatggcgtt actgtacgta
    54721 aagtgattta cgtaccaggt aaactcctca atctggtcgt tggctaagcg cgggaggaag
    54781 cgtgcgatat ctggcaacac tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg
    54841 ctggcatctg cgtgacacca cgcaggttcc ttccactatg aaggtcatga tcctggactc
    54901 aggcgatccg aacgggccat taagccgtgc ggtgcgtaac cagttacgtc tgaatggtgt
    54961 cgagttgctt gataaagaaa ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt
    55021 gagcatcgcg aaagataccg catcggtatt ccgtaacggt caaacagcag agtatcagat
    55081 gatcatgacg gtgaatgcga ccgtgttgat ccccggccgt gatatctacc cgattagcgc
    55141 caaagtcttc cgttcattct tcgataaccc gcaaatggcg ttagcgaaag ataacgaaca
    55201 agacatgatc gtaaaagaga tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc
    55261 aagcatccgt gctgcggata ttcgttccga cgaagaacag acgtcgacca caacggatac
    55321 tccggcaacg cctgcacgcg tctccaccat gctgggtaac tgatgattcg gttgtacccg
    55381 gaacaactcc gcgcgcagct caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac
    55441 gatcctctgt tattgcagga aagccaggac gctgttcgcc aggtagctgc ggcacaagga
    55501 ttcgaagaac accacacttt ttccattgat cccaacactg actggaatgc gatcttttcg
    55561 ttatgccagg ctatgagtct gtttgccagt cgacaaacgc tattgctgtt gttaccagaa
    55621 aacggaccga atgcggcgat caatgaacaa cttctcacac tcaccggact tctgcatgac
    55681 gacctgctat tgatcgtccg cggtaataaa ttaagcaaag cgcaagaaaa tgccgcctgg
    55741 tttactgctc ttgcgaatcg cagcgtgcag gtgacctgtc agacaccgga gcaggctcag
    55801 cttccccgct gggttgctgt gcgcgcaaaa cagctcaact tagaactgga tgacgcggcg
    55861 aatcaggtgc tctgctactg ttatgaaggt aacctgctgg cgctggctca ggcactggag
    55921 cgtttatcgc tgctctggcc agacggcaaa ctgacattac cgcgcgttga acaggcggtg
    55981 aatgatgccg cgcatttcac cccttttcat tgggttgatg ctttgttgat gggaaaaagt
    56041 aagcgcgcgt tgcatattct tcagcaactg cgtctggaag gcagcgagcc ggttattttg
    56101 ttgcgcacat tacaacgtga actgttgtta ctggttaacc tgaaacgcca gtctgcccat
    56161 acgccactgc gtgcgttgtt tgataagcat cgggtatggc agaaccgccg gggcatgatg
    56221 ggcgaggcgt taaatcgctt aagtcagccg cagttacgtc aggccgtgca actcctgaca
    56281 cgaacggaac tcaccctcaa acaagattac ggtcagtcag tgtgggcaga gctggaaggg
    56341 ttatctcttc tgttgtgcca taaacccctg gcggacgtat ttatcgacgg ttgatatgaa
    56401 atctttacag gctctgtttg gcggcacctt tgatccggtg cactatggtc atctaaaacc
    56461 cgtggaaacg ctggcgaatt tgattggtct gacgcgggtc acaatcatcc ctaataatgt
    56521 tcctccgcat cgtccccagc cggaagcgaa cagcgtgcag cgtaaacaca tgcttgaact
    56581 ggcgattgcc gacaagccat tatttactct tgatgaacgc gagctaaagc gcaatgcccc
    56641 ctcttacact gcgcaaacac tgaaagagtg gcggcaggaa caaggaccgg acgtgccgct
    56701 ggcgtttatt attggtcagg attcattgct gacctttccg acctggtacg aatacgaaac
    56761 gatactcgac aatgcacatt tgatcgtctg tcggcgtcca ggttacccac ttgaaatggc
    56821 gcaaccgcaa taccagcaat ggctggaaga tcatttgaca cataacccgg aagatcttca
    56881 ccttcagcct gccggtaaaa tttatctggc tgaaacgccg tggtttaaca tctcggcgac
    56941 catcatccgc gaacgtttgc aaaacggtga atcgtgtgag gatttattgc cggaaccggt
    57001 attgacttac attaaccaac aaggcttgta tcgctgatac ctgtcgttac ggagggcatg
    57061 atgcgactgt ggttaattcg tcatggcgaa acacaagcga atgtcgatgg tctgtacagc
    57121 ggtcatgcgc ccacacccct gaccgcacgc ggtattgagc aagcgcaaaa tctgcataca
    57181 ctgctagatg atgtttcctt tgacttggtt ttatgcagtg aactggaacg ggcacagcat
    57241 accgcgcgac tggttctcag tgaccgccag ctccccgttc atatcatccc tgaactcaac
    57301 gaaatgtttt ttggcgactg ggagatgcga catcatcgcg acctcatgca agaagatgcc
    57361 gaaaactata gcgcgtggtg caatgactgg cagcatgcta tccccacgaa cggtgaagga
    57421 tttcaggcat tttcgcaacg tgtggaacgc tttatcgcaa ggcttagtga atatcagcac
    57481 tatcagaata ttttaatcgt cagccatcag ggcgtactga gtctgttaat tgctcgttta
    57541 attggcatgc ctgccgagtc tatgtggcat tttcgcgttg accaggggtg ttggagcgcc
    57601 attgatatca accaaaaatt cgcgacgcta cgcgtcctca atagccgtgc catcggggtc
    57661 gagaacgcat gacttttctg tttttttacg ggtaagccgc aacgaccatt gacagccccg
    57721 ggcaggctga tattctccgc agccagactt tttccgccag acacgacttt gtagaaattg
    57781 ttttacaaaa atggcgatgc aatctgcggc gcggggtggg atgatagccc actttcgaaa
    57841 gccgattcgg cgacaattgt cccgaaatcg cctctggttc aggtatactg acagaccatt
    57901 tttatctatt tgattcaccc agggggaaaa cttgcagggt aaagcactcc aggattttgt
    57961 tatcgacaaa attgatgacc tcaaaggtca ggacatcatc gccttagacg ttcaaggcaa
    58021 atccagcatc accgactgca tgatcatctg tacgggtacg tccagccgtc atgttatgtc
    58081 cattgctgac cacgttgtgc aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga
    58141 aggtgaaaac agcgccgact ggattgtcgt ggatttgggc gatgtgattg tccatgtcat
    58201 gcaggaagag agccgtcgcc tgtatgaact ggaaaaactc tggagttaat gcgtgaagct
    58261 gcaacttgtc gccgtgggaa cgaaaatgcc ggactgggta caaaccggtt ttaccgagta
    58321 cctgcgtcgt tttccgaaag atatgccctt cgagctgatt gaaattccgg ccggcaaacg
    58381 cggcaagaat gcggacatca agcgcatact cgacaaagag ggtgagcaga tgttggcggc
    58441 cgcaggcaaa aaccgcattg tcaccctcga tattccaggc aagccctggg atacgccgca
    58501 gttagccgct gagctggaac gctggaagct ggatggtcgc gacgtaagtc tactgattgg
    58561 cgggcctgaa gggttgtcgc ctgcctgtaa agcggcggct gagcagagct ggtcgctgtc
    58621 ggcgcttacc ctcccccatc cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc
    58681 gtggagcatc accaccaacc atccttatca ccgtgagtga taagggagct ttgagtagaa
    58741 aacgcagcgg atgaaactac agaactcttt tcgcgactat acggcagagt ccgcgctgtt
    58801 tgtgcgccgg gcgctggtcg cctttttggg gattttgctg ctgaccggcg tgcttatcgc
    58861 caacctgtat aatctgcaaa ttgttcgctt taccgactac cagacccgct ctaatgaaaa
    58921 ccgcattaag ctggtgccta tcgcgcccag ccgcggcatt atctacgacc gtaacggtat
    58981 ccctctggcc ctcaaccgca ctatctacca gatagaaatg atgccggaga aagtcgataa
    59041 cgtgcagcaa acgctggacg ctttgcgcag cgtggtagat ctgaccgatg acgatattgc
    59101 tgcattccga aaagagcgcg cacgttcaca ccgtttcacc tctattccgg tgaaaactaa
    59161 cctaaccgaa gtacaagtag ctcgctttgc cgtcaatcag taccgttttc cgggtgtcga
    59221 agttaaaggc tataaacgtc gttactatcc ttacggttcg gcgttgaccc acgtcatcgg
    59281 ctatgtgtcg aaaatcaacg ataaagacgt cgaacgcctg aataatgacg gcaaactggc
    59341 caactatgcg gcaacgcatg atattggtaa gctgggcatt gagcgttact atgaagacgt
    59401 gctgcacggt cagaccggtt atgaagaggt tgaagttaac aaccgtgggc gtgttatccg
    59461 ccagttaaaa gaagtaccac cgcaagccgg acacgatatt tacctgacgc tggatctcaa
    59521 actccagcaa tatattgaaa cgctgctggc gggtagccgc gcagctgtgg ttgttaccga
    59581 accgcgtaca ggtggggtgc tggcgctggt ttccacgcct agttatgacc caaacctgtt
    59641 tgttgacggt atctccagca aagattattc cgccttgctg aacgacccga atacaccgct
    59701 ggtgaaccgc gccacacagg gggtttatcc tcccgcgtct acagttaaac cctatgtggc
    59761 ggtttcggca ttaagcgccg gggtgatcac gcgcaatacg acgctgtttg acccaggctg
    59821 gtggcaacta ccagggtcgg aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg
    59881 tctgaatgtc acaagatcgc tggaagaatc tgcggatacc ttcttctatc aggtggccta
    59941 cgatatgggg atcgatcgcc tctccgaatg gatgggtaaa ttcggttatg gtcattacac
    60001 cggtatcgac ctggcggaag aacgttccgg caacatgcct acccgcgaat ggaaacagaa
    60061 acgctttaaa aaaccgtggt atcagggtga caccattccg gttggtatcg gccagggtta
    60121 ctggacagcg accccaatcc agatgagtaa ggcactgatg atcctgatta atgacggtat
    60181 cgtgaaggtt ccgcatttgc tgatgagcac cgccgaagac ggcaaacagg tgccatgggt
    60241 acaaccgcat gaaccgcccg tcggcgatat tcattccggt tactgggagc tggcgaaaga
    60301 cggtatgtac ggtgttgcta accgccctaa tggtacggcg aataaatact ttgccagcgc
    60361 accgtacaaa attgcggcga aatctggtac cgctcaggtc ttcggtctga aagcgaacga
    60421 aacctataat gcgcacaaaa ttgccgagcg tttacgtgac cacaaactga tgaccgcctt
    60481 tgcgccatac aacaatccgc aagtggctgt cgccatgatt ctggagaacg gcggcgcggg
    60541 tccggcggtt ggtacgctga tgcgccagat cctcgaccac attatgctgg gtgataacaa
    60601 caccgatctg cctgcggaaa atccagcggt tgccgcagcg gaggaccatt aatcatgacg
    60661 gataatccga ataaaaaaac attctgggat aaagtccatc tcgatcccac aatgctgctg
    60721 attttactgg cattgctggt ttacagcgcc ctggttatct ggagcgccag cggtcaggat
    60781 attggcatga tggagcgtaa aatcggccaa atcgcgatgg gtctggtcat catggtggtg
    60841 atggcgcaaa ttcctccacg cgtttatgaa ggctgggccc cctatctcta tatcatctgt
    60901 attattttgc tggtggcggt agatgctttc ggagccatct ctaaaggcgc tcaacgctgg
    60961 ctggacctcg gtattgttcg ttttcagccg tcggaaattg ccaaaatagc cgtaccactg
    61021 atggttgcgc gctttatcaa ccgcgacgtt tgcccgccat cgttgaagaa tactggcatc
    61081 gcgctggtgc tgatatttat gcccacgctg ctggtggctg cacagcctga cctgggaaca
    61141 tcaatcctcg ttgcgctttc cggtctgttt gtactgttcc tctctggcct tagctggcgt
    61201 ctgattggcg tcgcagtagt gctggtagcg gcgttcattc cgattctgtg gttcttcctg
    61261 atgcatgatt accagcgcca gcgcgtaatg atgctcctgg acccggaatc agacccactc
    61321 ggcgcgggct atcacattat tcagtctaaa attgctattg gctccggcgg attacgcggc
    61381 aaaggctggc tgcacggcac tcagtcacag cttgaatttc tccccgaacg ccatactgac
    61441 tttatcttcg cggtactggc ggaagagctg ggattagtgg gcattctgat tctgctcgct
    61501 ctctacattc tgctgatcat gcgcgggctg tggatagccg ccagagcgca aaccaccttt
    61561 ggtcgcgtca tggctggtgg cttaatgctg atattattcg tttatgtctt cgtaaatatt
    61621 ggtatggtaa gcggtattct gccggttgta ggggttccgc tcccactggt cagttatgga
    61681 ggatcggcgc taattgtgct gatggctggg ttcgggattg taatgtcaat ccacacccac
    61741 aggaaaatgt tgtcgaaaag cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat
    61801 ctgcatcgcg gcaggaatgc tcgcggcatg tacaagcgat gatggtcagc aacagacggt
    61861 aagtgtaccg cagcctgcgg tatgtaacgg ccccatagtt gaaattagcg gggcggaccc
    61921 gcgtttcgaa ccactgaacg cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta
    61981 caaaatcgtg caggatccgt ctcgatttag ccaggcggga ctggcggcaa tctatgatgc
    62041 cgaaccaggc agtaacctga cggcctctgg cgaagctttc gatccgacaa agctgacggc
    62101 ggcccatcca acgcttccga tccccagcta cgccagaatc actaacctgg ctaacgggcg
    62161 aatgatcgtg gtgcgcatta atgatcgcgg cccttacggc aacgaccgcg ttatttcgct
    62221 ttctcgcgcg gcagctgacc gtcttaacac gtcaaacaac accaaagttc gtatcgatcc
    62281 gattattgtc gcccaggatg gttcgctttc tggtcctggt atggcttgta ccacagtcgc
    62341 caaacagact tacgccctgc ctgcacctcc cgatttaagc ggtggcgcgg gaacaagttc
    62401 agtgtctggc ccgcagggtg acattcttcc ggtcagtaat tcgacgctaa aaagcgaaga
    62461 tccgaccggc gcgccggtaa ccagtagcgg tttcctcggc gctccaacga ccttagcgcc
    62521 tggtgtactg gaaggcagcg aaccgacgcc tgcgccacag cccgttgtta cagcttcgtc
    62581 gacaacgcct gcaacctcgc ctgcaatggt gacaccgcaa gccgcctcgc aaagcgccag
    62641 cggcaacttt atggtgcaag tcggggccgt aagcgatcag gctcgtgcgc aacagtacca
    62701 acagcaactg ggacagaagt tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg
    62761 gcggatccag cttggcccat tcgccagcaa agccgaagcc agtaccttgc agcaacgttt
    62821 gcaaaccgaa gcccaattac agtcatttat tactaccgcg cagtagcgta aagcaggcat
    62881 ctgatgtgtc aatttctgta aatgacgtta acaaagtcac gcggaaagtc agatgcctgc
    62941 cggtagtgca tttgctatag tagggcactt ttttaattcc atcacggatg tcgtagttca
    63001 gaccatgaat accatttttt ccgctcgtat catgaagcgc ctggcgctca ccacggctct
    63061 ttgcacagcc tttatctctg ctgcacatgc cgatgacctg aatatcaaaa ctatgatccc
    63121 gggtgtaccg cagatcgatg cggagtccta catcctgatt gactataact ccggcaaagt
    63181 gctcgccgaa cagaacgcag atgtccgccg cgatcctgcc agcctgacca aaatgatgac
    63241 cagttacgtt atcggccagg caatgaaagc cggtaaattt aaagaaactg atttagtcac
    63301 tatcggcaac gacgcatggg ccaccggtaa cccggtgttt aaaggttctt cgctgatgtt
    63361 cctcaaaccg ggcatgcagg ttccggtttc tcagctgatc cgcggtatta acctgcaatc
    63421 gggtaacgat gcttgtgtcg ccatggccga ttttgccgct ggtagccagg acgcttttgt
    63481 tggcttgatg aacagctacg ttaacgcact gggcctgaaa aatacccact tccagacggt
    63541 acatggtctg gatgctgatg gtcagtacag ctccgcgcga gatatggcgc tgatcggcca
    63601 ggcattgatc cgtgacgtac cgaatgaata ctcgatctat aaagaaaaag aatttacgtt
    63661 taacggtatt cgccagctga accgtaacgg cctgttatgg gataacagcc tgaatgtcga
    63721 cggcatcaaa accggacaca ctgacaaagc aggttacaat cttgttgctt ctgcgactga
    63781 aggccagatg cgcttgattt ctgcggtgat gggtggacgt acttttaaag gccgtgaagc
    63841 cgaaagtaaa aaactgctaa cctggggctt ccgtttcttc gaaaccgtta acccactgaa
    63901 agtaggtaaa gagttcgcct ctgaaccggt ttggtttagt gattctgatc gcgcttcgtt
    63961 aggggttgat aaagacgtgt acctgaccat tccgcgtggt cgcatgaaag atctgaaagc
    64021 cagctatgtg ctgaacagca gtgaattgca tgcgccgctg caaaagaatc aggtcgtcgg
    64081 aactatcaac ttccagcttg atggcaaaac gatcgagcaa cgcccgctgg ttgtgttgca
    64141 agaaatcccg gaaggtaact tcttcggcaa aatcattgat tacattaaat taatgttcca
    64201 tcactggttt ggttaaaaat tgaacacttg aaagtgtaat ttccgtcccc atatactaag
    64261 catcagtaaa aaaactcccg ccttctggcg ggagttgcta tttaattacg ttacgccgga
    64321 gctgacatga aaaccaaact taacgaactg cttgaattcc ctactccttt tacttacaaa
    64381 gttatggggc aggcgttacc tgagctggtt gatcaggtgg ttgaagtggt acagcgccat
    64441 gcgccaggtg actacacccc aacggtaaaa ccaagcagca aaggcaacta ccactcggta
    64501 tctatcacta tcaacgccac tcatatcgag caggttgaaa cactgtatga agaactgggc
    64561 aaaatcgata ttgtccgcat ggttctgtaa ctcgcttctc cgttacccgg ctcctggtcg
    64621 ggtaactccc ctctcctcgc tgtgatatac ttttccccca cttttactca ttctccacgg
    64681 agatgccgtt ttgtatcagg ataaaattct tgtccgccag ctcggtcttc agccttacga
    64741 gccaatctcc cgggctatgc atgaattcac cgatacccgc gataatagta cccttgatga
    64801 aatctggctg gtcgagcact atccggtatt cacccaaggt caggcaggaa aagcggagca
    64861 cattttaatg ccgggtgata ttccggtgat ccagagcgat cgcggtgggc aggtgactta
    64921 tcacgggccg ggacaacagg tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg
    64981 tgtgcgtgaa ctggtgacct tgcttgagca aacagtggtg aataccctgg ctgaactggg
    65041 tatagaagcg catcctcggg ctgacgcgcc tggtgtctat gtcggggaaa agaaaatttg
    65101 ctcactgggt ttacgaattc gacgcggttg ttcattccac ggtctggcat taaacgtcaa
    65161 tatggatctt tcaccatttt tacgtattaa tccttgtggg tatgccggaa tggaaatggc
    65221 taaaatatca caatggaaac ccgaagcgac gactaataat attgctccac gtttactgga
    65281 aaatatttta gcgctactaa acaatccgga cttcgaatat attaccgctt aattccatac
    65341 atcaatggcc caatttacac tgggtcatta ctcataatac ctgctaatgc tttacatctc
    65401 cttcgcgttt gtatattttc tttatcactt gcagaaatgc ggtgccacgc cttgctccgt
    65461 aactcacccc attgattaac ctattgaaat agttgattat ggtgagcaaa agcgttccgt
    65521 tccaattaga cataagatta ttgtaatttt ttaaatatta tttttccatg aataaaattg
    65581 gagtaagtgc gtggatagta ataatcaaat tgaaccctgt ttaagcagaa agtcgtcaga
    65641 aggtaaacca caaatattta caaccttacg aaatattgat cttaaccttc tgactatttt
    65701 tgaagctgta tatgtacata aagggatcgt taatgcagcg aaagtgctta atctgacccc
    65761 ctcggcaatc agtcagtcta ttcagaaact gcgcgttata ttccctgacc cattgtttat
    65821 tcgcaaaggc cagggtgtca ctcctaccgc atttgcgatg catctacatg agtatatcag
    65881 tcagggcctt gagtccattc ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg
    65941 aacgataact attgcgacca ctccctcggt cggagccctg gtccttcctg tcatctatcg
    66001 ggcgattaaa actcactatc cgcagctttt actgcgcaac ccgcccatca gcgacgcgga
    66061 aaaccaactc agtcagtttc aaaccgatct catcatcgat aacatgtttt gcaccaatcg
    66121 tacggtgcaa catcatgttc tgttcaccga caatatggtg ttaatttgcc gcaagggaaa
    66181 tccactactc tctttagaag atgacagaga gactatcgac aacgctgcgc atgtacttct
    66241 gttaccggaa gggcaaaatt tcagcggtct gcggcagaga gttcaagaga tgtttccgga
    66301 ccggcaaatc aatttcacca gctacaacat tttgacaatc gctgcactgg ttgccaacag
    66361 tgacatgtta gcgattattc ccagccgttt ttataacctg tttagtcgtt gctggccgct
    66421 ggaaaaactg ccttttccgt ccttaaatga ggagcaaata gatttctcta tccactacaa
    66481 caaattcatc ctgcgtgatc ccatcctgca cggagtaata gatgttatcc gtaatgcatt
    66541 ttgaaaaagt attgttacag ccagatcaca gcgcagaacg ataaaatggc acaatccaac
    66601 aacaatttta cattttcgcg accgctttgg ctgctttcag gtccgtttca atgatatact
    66661 gccagtcgtt aattcaaaaa tagttgataa ttacaacaat ctattgaatt gaaacgcttt
    66721 ccttcgtaat tcgcaactgg aacacgtacg ctatgagtaa acccattgtg atggaacgcg
    66781 gtgttaaata ccgcgatgcc gataagatgg cccttatccc ggttaaaaac gtggcaacag
    66841 agcgcgaagc cctgctgcgc aagccggaat ggatgaaaat caagcttcca gcggactcta
    66901 cacgtatcca gggcatcaaa gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg
    66961 aagcctcctg ccctaacctg gcggaatgct tcaaccacgg cacagcaacg tttatgatcc
    67021 tcggcgctat ttgtacccgc cgttgtccgt tctgtgatgt tgcccacggt cgcccggtag
    67081 ctcctgatgc caatgaacca gtgaaactgg cgcagaccat tgccgatatg gcgctgcgtt
    67141 atgtggttat cacctccgtt gaccgtgatg acctgcgcga tggcggtgcc caacactttg
    67201 cggattgtat tactgccatt cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc
    67261 cggatttccg cggtcgaatg gatcgtgctc tggatattct gactgcaacg cccccagatg
    67321 tgttcaacca taacctggaa aacgtaccgc gtatttaccg gcaggtacgg cctggcgcag
    67381 attacaactg gtcgctgaag ctgctggaac gctttaaaga agcgcatccg gaaatcccga
    67441 ccaagtctgg tctgatggtg ggtctgggtg aaaccaatga agaaattatt gaggtaatgc
    67501 gcgacctgcg ccgtcatggt gtgacgatgt taacgctggg gcaatatttg cagccaagcc
    67561 gccatcacct gccggttcaa cgttacgtta gcccggatga gttcgacgaa atgaaagccg
    67621 aagcgctggc gatgggcttt acccatgctg catgcggtcc gtttgtccgc tcttcttacc
    67681 acgccgattt gcaggcgaaa ggaatggaag ttaagtaatc ctgtaataat tacttacatc
    67741 tgtctgataa aaaacccgct ttttgaagcg ggttttttgt atcgaacaag atattgaggg
    67801 agcgtcctgc tcgccacgtc actctttatg agagagcttt tcagcctgaa gatcaacgtc
    67861 tgcgcctttt ttcgccgcag catcgtcatc attcatcgcc ttcttgaacc ctttaatggc
    67921 cgctccaagg tctccgccca gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag
    67981 cgccgcaact accagcagtt tggtaatact aatctcaccc atagatacct tcttgacata
    68041 taaccggcaa cagaacgcgc cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc
    68101 acacaatagc aatctgtaac aaggtgaatc aagcgccgag taaaaaaaca tcataataat
    68161 tgcggcggcg caaagcgacg gttgtttaag atgggcagtt gcgcgcgcac ctgacgcaca
    68221 cgttcgggcg tcacttccgc cataatgagt gcaggcattt ctgacgctgc cgcaatggtg
    68281 acgccaaagg gatctataat ccggctttgc ccgatatttt tgttcccgca ctcccccgcc
    68341 gccaccatat aacaggtggt atccagcgca cgagcggcaa gcaacgttga ccagtgatgc
    68401 tctttgagcg gcccacgaac ccaggcggca ggaagtacca ggatttcagc tccctgtaaa
    68461 gcctgtgcca gcgccagctc tggaaagcgt aagtcataac aggtcatcag accgaccttc
    68521 atcccttcca cctccagtaa cggagctatt tcattaccag catcaacatg gcgtgattcc
    68581 tgaatggcaa atgcatcata gagatgcagt ttggcataac gggcgacgat gtttcctgcc
    68641 tgaagtgcca ccagcatatt ccatgcgcgc cccggcgttg aaggaagatg aatcgtcaga
    68701 attgtcgtca tcatgttacg tttactttct cgccgtaaac gtccgaggaa ttcgccttcc
    68761 agcagctgtg ctgatttaac cgatagatct gcatcatgat catcgcgcgc cagcaatgct
    68821 tccggcagga caaacagcga tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa
    68881 atctcagcgt tcttttccca cacagatgta acagcaaact gtcctgcagc aaccagcatt
    68941 gccacctcct gtcagagtcg taaaattcgc caacgacgat tattcggcgt tacacttatc
    69001 actcatacaa atcaaatagc aggattttgc agtgttacaa cttcttttag cagtttttat
    69061 tggcggtggt acgggaagcg tagcgagatg gctgttaagt atgcgattta acccgctgca
    69121 tcaggcgatt ccgttgggga cgctggcagc aaacctgatt ggggcattca tcataggaat
    69181 gggattcgcc tggttcagca ggatgacgaa cattgatcca gtgtggaaag tattaatcac
    69241 caccggattt tgtggcggtc taacaacctt ctcaacattt tcggcagaag tggtgttttt
    69301 gttacaagag ggccgctttg gctgggcatt actgaacgtt ttcgtcaacc ttctggggtc
    69361 ttttgccatg accgcactgg cattctggct gttttcggcc tcaaccgtac actaaagacc
    69421 aaaaaaaacc cgctgattaa gcgggttttg aattcttgct gacgtatctt acagagcgat
    69481 tacgtttgca gcagaagggc ctttggcacc gttagtgatt tcgaactcta cgcgctgacc
    69541 ttcagcaaga gttttaaaac cattagtctg gattgcagag aagtgtacga acacgtcttt
    69601 gctgccgtct tccggagtaa tgaaaccgaa tcctttggac tcattaaacc acttaacgtt
    69661 acctttaatc ttagacatca aaattacctt tacatgaaaa atagacacaa atgctgtgtc
    69721 ggttatcagt acaccaattg tggtacgctt tgtccagtgc aactttacta aaaagtgata
    69781 aaagtcgcgt tatttttgat gttgcttatt ttagttattg attttaaaga agttactaaa
    69841 acttcatttg tctcaaaact gaaagcgcat ccaggcaaag tacacattgc cattgttgta
    69901 ggtacccgga atgtaggtca tctgaaaagt cactgggcca taacccacgg aggccaatgg
    69961 cagtagaacc gggagaggga tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa
    70021 tcccagacct aaatgaaaat tttcatccgc cagcggtcgc caggtacttt cccatccgta
    70081 tccggcaatc ggttcccatt tgttccacga gtccttaaat gccatggcat acaggccatg
    70141 ccagtttcct ttttcatccc aacgcgacag gccaaaaccg ccaccccacg gtcgctcgtt
    70201 atagcgatcg gttttttctt tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat
    70261 atataaatca taatgttcag gctgttgcca ggtttgtgca atattttctc taaacgttgt
    70321 catcctctca tctgcgttag caaaaacttt accaacgctg attaactgaa taaaaacaaa
    70381 ggaaaagata gcgacatatt tactcacgtt catttgtgac cataaaacat ttatcaaaaa
    70441 tctactacta gcatagcaaa gctacaatta acataacctt aatagaccca acataaagaa
    70501 taatctgaat agctgctcta tttatttccg cttgttcaag ttaaatattt ccgcgcatcg
    70561 attaaagatg atgcgtcgtt ttctgcgatg ggaatagtca aaaaagaaaa accaagtctt
    70621 ttttgatgac aaaatgcaat caaggaaaaa ttattttatt tttttaacga gatacaacaa
    70681 tcatcttaac gaagtatata atatatatcg tcaacccatg ctcatattag attatttttt
    70741 gagcgcacag ccgcagctaa caccattgca attaacaaat ttgcatcaat ccaccatcaa
    70801 tttacacaca ttattatgtg ataattgcca accgctaaat atgcgttttg ttatctatgt
    70861 ataaaaacag caacttcaat gtcttaatgg cagtttttct tgattttaat cagcattcat
    70921 cgccaattta ttgggcatat tttttcctta agctttagga agtttttatt atttactttg
    70981 ggcctggaga caggaaaaat tatgctgaca ttcattgaac tccttattgg ggttgtggtt
    71041 attgtgggtg tggctcgcta catcattaaa gggtattccg ccactggtgt gttatttgtc
    71101 ggtggcctgt tattgctgat tatcagtgcc attatggggc acaaagtgtt accgtccagc
    71161 caggcttcaa caggctacag cgccacggat atcgttgaat acgttaaaat attactaatg
    71221 agccgcggcg gcgacctcgg catgatgatt atgatgctgt gtggctttgc cgcttacatg
    71281 acccatatcg gcgcgaatga tatggtggtc aagctggcgt caaaaccatt gcagtatatt
    71341 aactcccctt acctgctgat gattgccgcc tattttgttg cttgtctgat gtcactggcc
    71401 gtctcttccg caaccggtct gggggttttg ctgatggcaa ccctgtttcc ggtgatggta
    71461 aacgttggta tcagtcgtgg cgcagcagct gccatttgtg cctccccggc ggcgattatt
    71521 ctcgcaccga cttcagggga tgtggtgctg gcggcgcagg cttccgaaat gtcgctgatt
    71581 gacttcgcct tcaaaacgac gctgcctatc tcaattgctg caattatcgg catggcgatc
    71641 gcccacttct tctggcaacg ttatctggat aaaaaagagc acatctctca tgaaatgtta
    71701 gatgtcagtg aaatcaccac cactgccccc gcgttttatg ccattttgcc gttcacgccg
    71761 atcatcggtg tactgatttt tgacggtaaa tggggtccgc aattacacat catcactatt
    71821 ctggtgattt gtatgctgat tgcctccatt ctggagttcc tccgcagctt taatacccag
    71881 aaagttttct ctggtctgga agtggcttat cgcgggatgg cagatgcgtt tgctaacgtg
    71941 gtgatgctgc tggttgccgc tggggtattc gctcaggggc ttagcaccat cggctttatt
    72001 caaagtctga tttctatcgc tacctcgttt ggttcggcga gtatcatcct gatgctggta
    72061 ttggtgattc tgaccatgct ggcggcagtc acgaccggtt caggcaatgc gccgttttat
    72121 gcgtttgttg agatgatccc gaaactggcg cactcttccg gcattaaccc ggcgtatttg
    72181 actatcccga tgctgcaggc gtcaaacctg ggtcgtaccc tatcacccgt ttctggcgta
    72241 gtcgttgcgg ttgccgggat ggcgaaaatc tcaccatttg aagtcgtaaa acgcacctcg
    72301 gtgccggtgc ttgttgggct agtgattgtt atcgttgcta cagagctgat ggtgccagga
    72361 acggcagcag cggtcacagg caagtaaata gtaatgccgg aggcaagttt cctccggctt
    72421 tatcatgtca cccactgtgg tatatgcgtt gtggtctgcc aactttgccg tgaacaattt
    72481 cagcaataat cagatggcgg ctggcgcaat attcaagata acgcctggca gtggtgcggc
    72541 tgatggtcag tgcctgcgcc accgtttctg ccgtatgttg cacaccaggc tctttaaaca
    72601 gttttcgcac cgcgtttagc gtcaaggggt caatgccggt cggcagttcg tctttgggtt
    72661 caccgcgagc ataggcatta aacatctcat caatttgctt ctggctggcg ctatcaatac
    72721 tttccagcat atgtttacgc tggcggaaac gggttagcgt ttgccccagt gtattgattc
    72781 acttgaagta cgaaaaaacc gggagaacat tggattattc gggatctgat gggattagat
    72841 ttggtggggc ttgcaagcct gtagtacaaa ttttaggcgt taaccaatga aacgcgaaag
    72901 atagtaaaaa attgcttttg tttcattgaa aaaacgaaaa acaaaaacac tataaatcat
    72961 ttcaatcata gcttcaaaaa acgttcaaaa ccgataacaa ccaggctgtc accaaatgac
    73021 tcatatcaca aatcagctca tgccgtttag gtatgttaca tgtgtgatta tgtgaggtga
    73081 agtatgtttt agctggctca tggttgttat acggcttttt tcgcatcttg tggttccaac
    73141 aaaggagcta caggctcttc ctgtttatga acaccataca ttgacaaaat ctcttggcga
    73201 gtttctggag gtaatttatg cagcgccaaa gctgtttgta gtgtttggcg atcaataccc
    73261 agcgttgcca atagggtgtc aacggagatg gaggggcgtt cttgcattaa tccggaaagc
    73321 ccatatttag ccatgtttag ctctatctct cggacttcat tttctcctag tttattgatt
    73381 aacagggaga acattttcaa tgtatcagca ttcatttctt cgatagcaat tttttgcata
    73441 gccggtattg ccgggatata ttcaacccca ggcccgcgac gcccttcttg tgaacgcttc
    73501 ggccatcctt ctttctttgc tcgtttatgc accccctgta ttgtcccagg cattccatcc
    73561 agttcagtaa tttgtttggg tgtcatccac tggtttatct catttgcgct tttcataaac
    73621 taagtttacc agatttatct atataaaaat caatggatta caaaataaac cacattaact
    73681 gatataaacc ggtttggttt atttggtaat atataaaaca gataatcaag acgaattatt
    73741 tgttttggta accctttatc atacgcaata tggttggtgt gcgaaagatg agtaacgatt
    73801 ggcatcgggc cgacgtgatt gcggccctca aaaaaacagg aacccctctg gcaaaattgt
    73861 ccagacgttc aggttattca accgggacgc ttgcaaatgc attagtcagg ccatggccta
    73921 aaggtgaacg cattattgct gacgccttaa acatgaaacc tgaggaaatt tggcctagta
    73981 gatatctaag ggatcacaat gagagagtgg atatcagcaa ttgaatgcat gggtttatct
    74041 ggtttacctg gttcattaat gggggttcac aaaagagctc agagagaagg atggctcaag
    74101 agaagaagag aaggaattag aggggttgct tacgaatacc atatatcttc tcttcctaac
    74161 attgctcgag ctgaagtttt gctccaacaa ggaaaattta aaaccagtca gggatgtttt
    74221 gaaatcgccc gccccacgct ggaagctcat gattatgatc gtgaggcact atggagcaaa
    74281 tgggataaag ccagcgattc ccagcgcagc ctggctgaaa aatggttgcc ctcaattcag
    74341 gccacagacg aaatgctgaa ccaggggatt tcaacgaaaa cggcttttgc gaccgttgca
    74401 gggcattacc aggtcagcgc atccactttg cgggacaagt attaccaggt acagaagttt
    74461 gcgaagcctg actgggcggc tgcacttgtt gatggacgtg gagcatcccg tcgcaatgtt
    74521 cacaaaagtg aatttgacga ggatgcctgg cagtttctga ttgcagatta tctgcgaccg
    74581 gaaaaacccg ccttccgcaa atgttatgag cgtctggaac tggcagcccg cgagcatggc
    74641 tggagtattc cctcccgtgc cacggccttt cgccggattc agcaactgga cgaggcaatg
    74701 gttgttgcct gtcgtgaagg tgaacatgca ctgatgcatc tgataccggc acagcagcga
    74761 actgtggaac acctggacgc catgcagtgg atcaacggcg acggttatct gcataacgtc
    74821 tttgtacgct ggtttaacgg tgatgtgatc cgtccgaaaa catggttctg gcaggatgtg
    74881 aaaacccgaa aaattctggg ctggcgctgc gatgtgagcg agaacattga ttcaattcgc
    74941 ctctcgttca tggatgttgt gactcgctac agtatcccgg aggattttca catcaccatt
    75001 gataacaccc gtggtgctgc gaataaatgg ctgacgggag gcgcgcccaa tcgctaccgc
    75061 tttaaggtaa aagaggacga tccaaaagga ctgtttttac tgatgggtgc gaaaatgcac
    75121 tggacaagcg ttgttgccgg taaaggctgg ggccaggcaa aacctgttga acgtgctttc
    75181 ggtgttggtg ggcttgagga atacgttgat aagcatccgg cactggctgg cgcatatacg
    75241 gggccaaatc cgcaggcaaa acctgataac tatggcgacc acgctgttga tgcagagctg
    75301 tttctgaaaa cctttgccga aggtgtggcg atgttcaatg ccagaacagg ccgtgaaaca
    75361 gaaatgtgcg ggggcaaact ctcgtttgat gatgttttcg agcgtgaata cgccagaacg
    75421 attgtgcgta agccaaccga agaacaaaaa cggatgctgt tactgcctgc cgaggcggtg
    75481 aacgtttcac gcaaaggcga gtttacgctt aaagttggcg gctcccttaa aggcgcgaaa
    75541 aacgtttatt acaacatggc attaatgaat gccggagtga aaaaagttgt ggtcagattt
    75601 gatccacagc agttacacag cacggtttat tgag
//
LOCUS       DAETQL010000004        72977 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.004, whole genome shotgun sequence.
ACCESSION   DAETQL010000004 DAETQL010000000
VERSION     DAETQL010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.004"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="K0W65_000277"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="K0W65_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBI3093688.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="K0W65_000278"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="K0W65_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBI3093689.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="K0W65_000279"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="K0W65_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBI3093690.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="K0W65_000280"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="K0W65_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBI3093691.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="K0W65_000281"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="K0W65_000281"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBI3093692.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="K0W65_000282"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="K0W65_000282"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBI3093693.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="K0W65_000283"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="K0W65_000283"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBI3093694.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="K0W65_000284"
     CDS             complement(4598..5320)
                     /locus_tag="K0W65_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3093695.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="K0W65_000285"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="K0W65_000285"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBI3093696.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="K0W65_000286"
     CDS             6520..6738
                     /locus_tag="K0W65_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBI3093697.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="K0W65_000287"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="K0W65_000287"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBI3093698.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="K0W65_000288"
     CDS             complement(7472..7672)
                     /locus_tag="K0W65_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBI3093699.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="K0W65_000289"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="K0W65_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="K0W65_000290"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="K0W65_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBI3093700.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="K0W65_000291"
     CDS             10101..12014
                     /locus_tag="K0W65_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3093701.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="K0W65_000292"
     CDS             12014..13036
                     /locus_tag="K0W65_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBI3093702.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="K0W65_000293"
     CDS             13030..13248
                     /locus_tag="K0W65_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBI3093703.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="K0W65_000294"
     CDS             13302..14171
                     /locus_tag="K0W65_000294"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBI3093704.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="K0W65_000295"
     CDS             complement(14226..14630)
                     /locus_tag="K0W65_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBI3093705.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="K0W65_000296"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="K0W65_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBI3093706.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="K0W65_000297"
     CDS             15615..17705
                     /locus_tag="K0W65_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBI3093707.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="K0W65_000298"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="K0W65_000298"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBI3093708.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="K0W65_000299"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="K0W65_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBI3093709.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="K0W65_000300"
     CDS             complement(19673..20275)
                     /locus_tag="K0W65_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBI3093710.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="K0W65_000301"
     CDS             complement(20265..20432)
                     /locus_tag="K0W65_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBI3093711.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="K0W65_000302"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="K0W65_000302"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBI3093712.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="K0W65_000303"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="K0W65_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBI3093713.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="K0W65_000304"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="K0W65_000304"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HBI3093714.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="K0W65_000305"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="K0W65_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBI3093715.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="K0W65_000306"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="K0W65_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBI3093716.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="K0W65_000307"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="K0W65_000307"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBI3093717.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="K0W65_000308"
     CDS             28269..28436
                     /locus_tag="K0W65_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HBI3093718.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="K0W65_000309"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="K0W65_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="K0W65_000310"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="K0W65_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HBI3093719.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="K0W65_000311"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="K0W65_000311"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HBI3093720.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="K0W65_000312"
                     /pseudo
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="K0W65_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="K0W65_000313"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="K0W65_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HBI3093721.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="K0W65_000314"
     CDS             complement(33764..34849)
                     /locus_tag="K0W65_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBI3093722.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="K0W65_000315"
     CDS             complement(34861..36165)
                     /locus_tag="K0W65_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HBI3093723.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="K0W65_000316"
     CDS             complement(36177..36530)
                     /locus_tag="K0W65_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBI3093724.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="K0W65_000317"
     CDS             complement(36541..37419)
                     /locus_tag="K0W65_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBI3093725.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="K0W65_000318"
     CDS             complement(37416..38642)
                     /locus_tag="K0W65_000318"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBI3093726.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="K0W65_000319"
     CDS             complement(38643..39806)
                     /locus_tag="K0W65_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HBI3093727.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="K0W65_000320"
     CDS             complement(39890..40252)
                     /locus_tag="K0W65_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBI3093728.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /locus_tag="K0W65_000321"
     CDS             complement(40269..41174)
                     /locus_tag="K0W65_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093729.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="K0W65_000322"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="K0W65_000322"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBI3093730.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="K0W65_000323"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="K0W65_000323"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBI3093731.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="K0W65_000324"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="K0W65_000324"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBI3093732.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="K0W65_000325"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="K0W65_000325"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBI3093733.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46122)
                     /gene="damX"
                     /locus_tag="K0W65_000326"
     CDS             complement(44830..46122)
                     /gene="damX"
                     /locus_tag="K0W65_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBI3093734.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="K0W65_000327"
     CDS             complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="K0W65_000327"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBI3093735.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="K0W65_000328"
     CDS             complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="K0W65_000328"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBI3093736.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="K0W65_000329"
     CDS             complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="K0W65_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBI3093737.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="K0W65_000330"
                     /pseudo
     CDS             complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="K0W65_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="K0W65_000331"
     CDS             complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="K0W65_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBI3093738.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="K0W65_000332"
     CDS             complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="K0W65_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HBI3093739.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="K0W65_000333"
     CDS             complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="K0W65_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBI3093740.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51689..54241
                     /gene="mrcA"
                     /locus_tag="K0W65_000334"
     CDS             51689..54241
                     /gene="mrcA"
                     /locus_tag="K0W65_000334"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBI3093741.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="K0W65_000335"
     CDS             complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="K0W65_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBI3093742.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55286..57421
                     /gene="igaA"
                     /locus_tag="K0W65_000336"
     CDS             55286..57421
                     /gene="igaA"
                     /locus_tag="K0W65_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBI3093743.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57486..58154
                     /gene="yrfG"
                     /locus_tag="K0W65_000337"
     CDS             57486..58154
                     /gene="yrfG"
                     /locus_tag="K0W65_000337"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBI3093744.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58165..58566
                     /gene="hslR"
                     /locus_tag="K0W65_000338"
     CDS             58165..58566
                     /gene="hslR"
                     /locus_tag="K0W65_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBI3093745.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58591..59460
                     /gene="hslO"
                     /locus_tag="K0W65_000339"
     CDS             58591..59460
                     /gene="hslO"
                     /locus_tag="K0W65_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBI3093746.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59604..61355)
                     /locus_tag="K0W65_000340"
     CDS             complement(59604..61355)
                     /locus_tag="K0W65_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBI3093747.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            61734..63356
                     /gene="pckA"
                     /locus_tag="K0W65_000341"
     CDS             61734..63356
                     /gene="pckA"
                     /locus_tag="K0W65_000341"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBI3093748.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63432..64784)
                     /gene="envZ"
                     /locus_tag="K0W65_000342"
     CDS             complement(63432..64784)
                     /gene="envZ"
                     /locus_tag="K0W65_000342"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBI3093749.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64781..65500)
                     /gene="ompR"
                     /locus_tag="K0W65_000343"
     CDS             complement(64781..65500)
                     /gene="ompR"
                     /locus_tag="K0W65_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBI3093750.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65728..66204
                     /gene="greB"
                     /locus_tag="K0W65_000344"
     CDS             65728..66204
                     /gene="greB"
                     /locus_tag="K0W65_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBI3093751.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66301..68622
                     /locus_tag="K0W65_000345"
     CDS             66301..68622
                     /locus_tag="K0W65_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBI3093752.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69060..69287
                     /gene="feoA"
                     /locus_tag="K0W65_000346"
     CDS             69060..69287
                     /gene="feoA"
                     /locus_tag="K0W65_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBI3093753.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69304..71625
                     /gene="feoB"
                     /locus_tag="K0W65_000347"
     CDS             69304..71625
                     /gene="feoB"
                     /locus_tag="K0W65_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBI3093754.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71625..71861
                     /gene="feoC"
                     /locus_tag="K0W65_000348"
     CDS             71625..71861
                     /gene="feoC"
                     /locus_tag="K0W65_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBI3093755.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72064..72951
                     /gene="rpnA"
                     /locus_tag="K0W65_000349"
     CDS             72064..72951
                     /gene="rpnA"
                     /locus_tag="K0W65_000349"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HBI3093756.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT        17590 a        19773 c        18456 g        17158 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacgatctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa agggcgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggtttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactccggc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcacgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 atcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 atttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacatcacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tggctgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aggaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgtgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gccatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggagcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact ctacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctag gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgccctcc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgcgccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaatgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc
    45301 cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac gaactgggcg
    45361 cgtagtggac ggacgttcag cggtttgcgt tttcgccgtg tcacgcgatg cattgccatt
    45421 gcgaacaggc gcaaccgttg cgggttcagt cggcaacgtg gaattgaccg ccacattgtt
    45481 cagctgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct gcacttcaac
    45541 acgttgttga ccatccgttg ccaccggggt ttgcccttga gtcggcgtag aagagatcgg
    45601 tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg ctggctgcac
    45661 gccattcgcc tgatcggtcg cattgccagc aagatcaata ctcttctcgc cagacgcggt
    45721 ttgatcgctg gaagtggtcg agggggcttt tagcgcagaa ccgataccga tgatcaacag
    45781 cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg gtttactggc
    45841 tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg tttcatcttc
    45901 ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt gcgcacgagt
    45961 cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct gcggttcgcc
    46021 acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac gacgatcgct
    46081 gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc ccactaaaag
    46141 gttaatgctt accacgttgc aattaacctg aagctaatag accgcttgat aagcggcctg
    46201 acctttcttg ttgttacgct gattgacaat cggcaatggc gttaagaaca agctcgtgcg
    46261 aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag cgcatctctc
    46321 ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc
    46381 gcggcccatt gaccggtaac ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg
    46441 cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata cccgcagcga
    46501 ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca tgaccaaagg
    46561 tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt
    46621 ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg tccagacgca
    46681 acaacgcatc cagattctct tccagccagt taaaaaacgc accgtcaaga ataatgccgt
    46741 atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt ttcagacagt
    46801 cgagatccac caccactgaa gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg
    46861 gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg
    46921 ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc
    46981 ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggc ttttgtaaca
    47041 acgccgtaaa gacggtatcg agtacagcta ggcttttata ctgctcgccg tcagggagga
    47101 taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta tcgagataca
    47161 gaggagccag ggtttcgctg gtgaccaaca tgacctgctc gcccgatttc agcggtaaga
    47221 atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta cgttccccga
    47281 gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac ccgcagacga
    47341 gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt ttgcaaccac
    47401 tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat acagcggatt
    47461 gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg
    47521 gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atggtcgttt caagataaac
    47581 gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag agccgccgcc
    47641 agtagccagc acaataccct gtttctcggt caactcattg atgacctttt cttcgcgatc
    47701 gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc cggttcgttt
    47761 ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta actggcgccc
    47821 aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt tctctgccat
    47881 tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca gagagcgccg
    47941 caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa ttatctcaat
    48001 actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct ggtcgctgca
    48061 agcgcgctgc cttgctacca ccgctctggc gataaatcac cgggtaagat tagcgtaaaa
    48121 aagacagcaa aatgccgcct gaatgataaa tcatcatcat ggaacgcagg cagcgaagag
    48181 caccaactct caaatcggta ctccttgtat gctaaatacc tgcgcgcgtc aaatagatga
    48241 aacacgttca gcataaaaac attaccgctt ttacggctgt ttactcactg gaaaccagtc
    48301 gtggcgtgat aaacaccact aactcgcgtc gttcatcttc ttttccgtca tgacgaaata
    48361 attgcccgaa ccagggaatg tcgccaagca acggtacgct atcctgaccc gatttatttt
    48421 tacgggtaaa aatgccgccc agcgccaacg tttctccgct ttttacctcg acctgcgttt
    48481 cgatctcctg cttatcaatc gccagcactt cgccatcggc ctgctgtagc acctgccccg
    48541 gaacgttctg gctgatgtgt aatttcagcc ggatgcgacc tttttgtaac accgtgggcg
    48601 tgacctccat ccccaggacg gcctctttaa attccaccga cgtcgcgcca ctttccccgc
    48661 tggaaacctg atatggaatt tcgctctcct gtttaatgct ggcaggctga agatgtgagg
    48721 ccagcagacg cggactggcg ataatatcca gctgctgttt ttgttcgagc gcggaaagct
    48781 caagatccag caagcgtccg ttgatgcgcc caatgttaaa accgacatgc gttgtcgccg
    48841 tcgctacgga gaggtcgcta ccaagcgtgg tgacttgccc aacgccacca gcgtgttgcg
    48901 catcggccag cgtccatttc acgcctaact cacgcaaact tttttcatta atggtgacaa
    48961 tatgcgccga cagctcaacc tgcccgaccg gcagatccat ttgcgctacc cactgttcaa
    49021 gcgcgcttaa cgccgttttg ttatctcgta gcaaaaggcg attggtgcgt ttatcgacgg
    49081 tcatactccc tttggcactc agtagcttct cccccgcttt cgccagttct cccgcgtcgg
    49141 cgtattgcag ggttatactg cgattttcca gcggcagatt tgcctgcgcc cgcgcctgct
    49201 ccgcctcctg gcgggcgata ttgtcattct gccaggcaat ggaatgcact gagagaatgt
    49261 tgccttcctg ccgcgttatc agtccggcgc ttttcactac agtttgtagt gcctgcttcc
    49321 agggaacatc tgttagatgt aacgacaccg taccgctgac gtctggcgac acgaccaggt
    49381 tcaacttctc ctgttcagcc agcgcctgca acacctgagc taccggaacg tcatccacca
    49441 tcagcgtcac tttttgcggc tttgccgcct gtacgccggg tatcagcatc aacagtagtg
    49501 cggctatcca ttgcttcatt tgtatctcct tgccgttgcc acaaccattg tggcggttcg
    49561 cagtttgtcc cggtacccag cgttagtacg tctggcgtca gctgtaaaat tgtccagccg
    49621 ttttccagca catcgttttg ctgcacccgt cgccatttct tttgcccgtc ttttattaca
    49681 ccgatgatgc gctcgcctcg ccctaccatc ccctgatagc gccactggct aagttcgcta
    49741 atccggcata tagatcttcc ggcgatttaa aagggtcacg cataccggtt aaaaggcaca
    49801 atgcaatacc tgccaacaac cagcgtttaa ccctcatgcg gcatctccag ttgtagcgtg
    49861 aacaaaagat catcaccttc cacgcttaac gaaaaacggc tcacgctgac gttgcgctct
    49921 gccagccgtg taaatgccga cggcactgct tcccacagcg ttttcaacgc caactcacct
    49981 ccctgcgcgg atggatgcca ggaaaccagt tgcgcgccgg ataactgaaa atccagtggc
    50041 gaaaagggca gcgttttttc ctcgctgaag ggagtggtgt ctaccaggcg atacagtgcg
    50101 gcccactgct ggtgatgact tgcccgtagt cgaattaatg cgtcgcgctc ttcatggtgt
    50161 gtcgatgata gaaaaatgag cgtaactaac atcagcaacc agaatgccca gcaaaactgg
    50221 cggaggcggg gtgatgtggc gaaccaccag tcaaagaaca tgttcatcgc taaccttcct
    50281 tgttaactga tactcaaatt gccagcgtcc ctgcgcatcc tgctgcgtgg ctccccgctg
    50341 attgagatga aaagacgcat cctggcggag tgacgtttct agtgcgttta acgcggtaat
    50401 gcttgttgtc agccccttga tctccagcgt tccctgctgc cagcttatcg ttgtcagcca
    50461 ggcgtgctcg ggtaaaagcg ccgccagtgc ttccagcgca gattgccagt cgcgggtaaa
    50521 ttgtcgctgg cgctgccgtt gcgaacgctg ttcgcgtaac tgctgttgct ccagcaaacg
    50581 tggcttcgtt atctgtaagc tgcgggcgag ttgttgttcc gcctgaagca aaactgcgtc
    50641 tatgcgcgct tcggcgctgc ctgtcagacg cagtattagc gttatcccga cggccagcag
    50701 cagaggcgca acgaacatca gcaaccagaa acgcagaaaa gcggtccggc gttgctgtcg
    50761 ccagggcaaa aaattaattg gcggattcat cagtacgcct tcccaagcgc cagccccagc
    50821 gcgatggcaa agtctccacc aggcggcggt agcggcggct gacgaacaga aacggcctcc
    50881 caggggtcaa atccgccttc gccacatatc gcgacgctgt cgggatcaac ggataacgct
    50941 gccgccagct ccttcgcgct agtcatcccc accgccagtt tgcgccccca gctatagcgc
    51001 gtcgcccaca gccactgttc gttatcacgc caggccagac attgctgatg agaaggtaaa
    51061 aaaggcagga atcgctgtaa tgcactggca tccggggtga tcgcactaac atgaacacgc
    51121 aacctttctg ccagcgtaag cagcgttgcc agctctttgc tttgcgcggc agtcacgtta
    51181 taagcggggc tgagtgagtc ttcgctgtaa tcgaagcgca gggagtccgg atccatatcc
    51241 agctcgcggg ccatcgtccc tgacagccag gccgtttgct cccgctcacc aagggacatc
    51301 gacgggcgcg gaaatgaccg ctgtaatgtg cgactggcgg gaaacgccaa cataatgtga
    51361 tgacgctgcg gcagttcgcg actccacggt aacaacgttt tagccagctg ctgcgcatca
    51421 acaatccgcc catctttgat aatgtcgtgc gccagcggca accgccacca gcgttgcaaa
    51481 aagcattctt ttgcgccccg cacgatcgca accgctgccg cttcttgctg ttgtaaatgc
    51541 aaaccaattt gccagatctt aaatgtcatt gtgatgatct ccttatcacc cgtcactctg
    51601 acgggtatat caatgcgtct ggcttgcctt tatactaccg cgcgtttgtt tataaactgc
    51661 ccaaatgaaa ctaaatggga aatttccagt gaagttcgta aagtattttt tgatccttgc
    51721 agtctgttgc attctgctgg gagcaggctc gatttatggc ctataccgct acatcgagcc
    51781 acaactgccg gatgtggtga cattaaaaga tgttcgcctg caaattccga tgcagattta
    51841 cagcgccgat ggcgagctga ttgctcaata cggtgagaaa cgtcgtattc cggttacgtt
    51901 ggatcaaatc ccaccggaga tggtgaaagc ctttatcgcg acggaagaca gccgcttcta
    51961 cgagcatcac ggcgttgacc cggtggggat cttccgtgcg gcaagtgtgg cgctgttctc
    52021 cggccacgcg tcacaagggg cgagtaccat tacccagcag ctggcgagaa atttcttcct
    52081 cagtccagaa cgcacgatga tgcgtaagat taaggaagtc ttcctcgcga ttcgcattga
    52141 acagttgctg acgaaagacg aaatcctcga gctttatctg aacaagattt accttggtta
    52201 ccgcgcctat ggtgtcggtg ctgcggcaca agtctatttc ggtaaaacgg tcgaccaact
    52261 gacgctgaac gaaatggcgg tgatagccgg gttgccgaaa gcgccttcca cctttaatcc
    52321 gctctactca atggatcgtg ccgtcgcgcg gcgtaacgtc gtactgtcgc ggatgctgga
    52381 tgaagggtat atcacccagc aacagttcga tcagacacgc actgaggcga ttaacgctaa
    52441 ttatcacgcg ccggagattg ctttctctgc gccgtacctg agcgaaatgg tgcgccagga
    52501 gatgtataac cgttatggcg aaagtgccta tgaagacggt tatcgcattt acaccaccat
    52561 cacccgcaaa gtgcagcagg ccgcgcagca ggcggtacgt aataacgtgc tggactacga
    52621 catgcgccac ggctatcgcg gcccggcaaa tgtgctgtgg aaagtgggcg agtcggcgtg
    52681 ggataacaac aagattaccg atacgctgaa ggcgctgccg acctatggtc cgctgctgcc
    52741 tgccgcagtc accagcgcca atcctcaggg agcgacggcg atgctggcgg acgggtcgac
    52801 cgtcgcattg agtatggaag gcgttcgctg ggcgcgtcct taccgttcgg atactcagca
    52861 gggaccgacg ccgcgtaaag tgaccgatgt tctgcaaacg ggtcagcaaa tctgggttcg
    52921 tcaggttggc gatgcatggt ggctggcaca agtgccggaa gtgaactcgg cgctggtgtc
    52981 gatcaatccg caaaatggtg ccgttatggc gctggtcggt ggctttgatt tcaatcagag
    53041 caagtttaac cgcgccaccc aggcactgcg tcaggtgggt tccaacatca aaccgttcct
    53101 ctacaccgcg gcgatggata aaggtctgac gctggcaagt atgttgaacg atgtgccaat
    53161 ttctcgctgg gatgcaggtg ccggttctga ctggcagccg aagaactcac caccgcagta
    53221 tgctggtcca attcgcttac gtcaggggct gggtcagtcg aaaaacgtgg tgatggtacg
    53281 tgcaatgcgg gcgatgggcg tcgactacgc tgcagaatat ctgcaacgct tcggcttccc
    53341 ggcgcaaaac attgtccaca ccgaatcgct ggcgctgggt tcagcgtcct tcaccccaat
    53401 gcaggtggcg cgcggctacg cggtcatggc gaacggcggc ttcctagtgg acccgtggtt
    53461 tatcagcaaa attgaaaacg atcagggcgg cgtgattttc gaagcgaaac cgaaagtagc
    53521 ctgcccggaa tgcgatattc cagtgattta cggtgatacg cagaaatcga acgtgctgga
    53581 aaataacgat gttgaagatg tcgctatctc ccgcgagcag cagaatgttt ctgtaccaat
    53641 gccgcagctg gagcaggcaa atcaggcgtt agtggcgaag actggcgcgc aggagtacgc
    53701 accgcacgtc atcaacactc cgctggcatt cctgattaag agtgctttga acaccaatat
    53761 ctttggtgaa ccaggctggc agggtaccgg ctggcgtgca ggtcgtgatt tgcagcgtcg
    53821 cgatatcggc gggaaaaccg gtaccactaa cagttcgaaa gatgcgtggt tctcgggtta
    53881 cggtccgggc gttgtgacct cggtctggat tggctttgat gatcaccgtc gtaatctcgg
    53941 tcatacaacg gcttccggag cgattaaaga tcagatctca ggttacgaag gcggtgccaa
    54001 gagtgcccag cctgcatggg acgcttatat gaaagccgtt cttgaaggtg tgccggagca
    54061 gccgctgacg ccgcctccgg gtattgtgac ggtgaatatc gatcgcagca ccgggcagtt
    54121 agctaatggt ggcaacagcc gcgaagagta tttcatcgaa ggtacgcagc cgacacaaca
    54181 ggcggtgcac gaggtgggaa cgaccattat cgataatggt gaggcacagg aattgttctg
    54241 attaaaaagg cgcttcggcg ccttttcagt tggctgacaa agtgcatttg tttatgccgg
    54301 atgcgggtga acgcgttatc cggctaacaa aatcgtgaaa aatcaatata ttgcaggaac
    54361 cccataagcc tgataagcgt tgtgcatcag gcaaacttca cgcatttaca ctcgcccctg
    54421 ccctttcaac cattcgcgca cgaggaacag cgcgctgaca ttacgcgctt cgttgaagtc
    54481 agggtcttcc agcaaatcca tcatatgcgc cagcggccag cgcacctgtg gtagcggctc
    54541 tggctcatcg ccttccagcg attccgggta gagatcttgc gcgaccacga tattcatttt
    54601 gctggaaaag taagacggtg ccatgctgag cttcttcaaa aaagtcagat cgttcgcacc
    54661 aaatccaacc tcttctttta gctcgcggtt agcggcttcg tagacgcttt cacccggatc
    54721 aattaatcct ttcgaaaaac ctaattcgta ggattcagtt cccactgcgt attcgcggat
    54781 caggatcagg tgatcgtcca caatcggcac aatcatcact gcttcccggt tggttggacg
    54841 cattcgttca taaacacgcc gcacgccatt gctgaactcc agatccacgc tctcgacggt
    54901 aaacagtcgg gaacgggcta cagtttcaac attcagaatg gtgggttttt gtaatgattt
    54961 gttcatcgtg ggatctatgc tgtgaaatca gcggttattg tgcgatatcg gacacgcttt
    55021 cggcaatgtg aattgcatgt tatttacatt tatgtaactt aataaataat cgtcctcaaa
    55081 tcaaattaaa agtcaatagg ttgaaataac tccaggaatt tgctgatatt ccgccttcag
    55141 agggtttgct atgatcagcg gttactgtga tgtgcttaat gatgctcaag ttaagctcca
    55201 cgcttgccga tagccaaccg cagaatcatg tattgtgtcc ggtgcgactg accacgcctg
    55261 acagactaag taagatgggg aaagcatgag caccattgtg atttttttag ctgctttgct
    55321 ggcctgctca ctacttgcgg gatggctgat aaaagtgcga tccagacggc gtcagctgcc
    55381 ctggaccaac gccttcgcgg atgcgcaaac gcgtaaactc acacctgaag aacgtagcgc
    55441 cgttgaaaat tatcttgaga gcctgacgca ggtattacag gtgcctggcc caacggaagc
    55501 tagcgcggca ccgatctctc tggcgctgaa tgccgaaagc aacaacgtca tgatgctgac
    55561 acacgctatc acacgttacg gcatctctac cgacgatccg aataaatggc gttactacct
    55621 cgattcggta gaagtccacc tgcccccctt ctgggaacag tacatcaacg atgagaatac
    55681 cgttgaactg attcataccg attcgctgcc gttggttatt tcactcaacg gtcatacgct
    55741 gcaggagtac atgcaggaaa cgcgcggcta tgccttgcaa cctgttccgt ccacgcaggc
    55801 gtcgattcgc ggggaagaga gtgagcaaat cgagctactc aatattcgca aagaaacgca
    55861 tgaagaatat gcgctgagtc gtccgcgcgg gctgcgtgaa gcgttgctga tcgtcgcctc
    55921 cttcctgatg ttctttttct gcctgattac cccggatgta tttgttccgt ggctggcagg
    55981 cggcgcatta ctgctgctgg gcgcaggtct gtgggggcta ttcgcgcccc cggcaaaatc
    56041 ctccctgcga gaaattcatt gtctgcgcgg tacaccccgt cgttggggat tgtttggcga
    56101 aaacgatcag gaacagatca acaatatttc actcggtatt atcgacctgg tctatcccgc
    56161 acactggcag ccatacattg ctcaggatct cggtcaacaa accgatattg atatctatct
    56221 cgaccgccac gtagtgcgtc aggggcgata tctttcgctg catgatgaag taaaaaactt
    56281 tccgttacag cactggctgc gcagtacgat tatcgctgcg ggctcgctgc tagtgctgtt
    56341 tatgctgtta ttctggatcc cgctggatat gccgctgaaa ttcactctct catggatgaa
    56401 aggcgcgcag accattgaag ccaccagcgt aaaacaactg gctgacgctg gcgtgcgggt
    56461 aggcgataca ttgcgtatta gcggtacggg aatgtgtaat attcgcactt ccggaacctg
    56521 gagcgcgaaa accaattcac cttttttacc gtttgactgc tcgcagatca tctggaatga
    56581 cgcccgctca ttaccattac cagaatctga actggtcaac aaagcgacgg cattgactga
    56641 agcagttaat cgccagctgc accctaaacc ggaagatgaa tctcgcgtca gtgcctcatt
    56701 acgttcagcg attcaaaaat ccggcatggt attgcttgat gattttggcg acattgtact
    56761 gaagacagcg gatttatgtt ctgccaaaga tgactgtgtg cgactgaaaa atgcgctggt
    56821 caatctcggc aacagtaaag actgggacgc gctggtaaaa cgcgccaacg ccgggaagct
    56881 cgatggcgtg aatgtgttat tacgcccggt gagtgctgaa tcgctggata acctggtggc
    56941 aacctccacc gcgccgttta tcacgcatga aacggcgcga gcggcacaat cactaaacag
    57001 tccggccccc ggcggattcc tgattgtcag cgacgaaggc agcgattttg ttgatcagcc
    57061 ctggccttcg gcatcacttt acgactaccc gccgcaagaa cagtggaacg ctttccagaa
    57121 actggcacaa atgctgatgc atacgccgtt taacgccgaa ggtatcgtca caaaaatctt
    57181 cactgacgcc aatggtacgc agcatattgg ccttcatccg atcccggatc gttccggcct
    57241 gtggcgctat ctcagcacca cattgctgct actgacgatg ctgggtagcg ccatttacaa
    57301 tggcgtacag gcctggcgtc gttaccagcg tcatcgcact cgcatgatgg agattcaggc
    57361 ctattatgaa agctgcctga acccgcaact gatcacccct tcagaaagcc ttatcgaata
    57421 acacgtttgc gcggcaggtt atgctaccct gtcgcgcaaa ttgcttcact ctggagattt
    57481 ccctcatgca tatcaacatt gcctggcagg acgtagatac cgttctgctg gatatggacg
    57541 gcacgttgct cgacctcgcc ttcgataact atttctggca aaagctggtg cctgaaacat
    57601 ggggcgcgaa aaacggggtt acgccacagg aagcgatgga atatatgcgc cagcaatatc
    57661 acgacgtaca gcatacgcta aactggtact gtcttgatta ctggagtgag caactgggtc
    57721 tggatatctg tgcgatgacc accgagatgg gaccgcgcgc cgtactgcgt gaagatacca
    57781 ttccgtttct tgaggcactg aaagccagcg gtaagcagcg aattttgctc accaatgctc
    57841 atccgcacaa cctggcggta aaacttgagc atacgggtct ggacgcacac cttgatttat
    57901 tactttccac ccacacattt ggttatccga aagaggatca gcggttatgg catgcggtgg
    57961 ccgaagctac gggtctgaaa gctgaaagaa cgctgtttat tgatgacagc gaagcgattc
    58021 tcgatgctgc cgcgcaattt ggtattcgtt actgcctcgg cgtgactaat cctgattccg
    58081 ggattgccga gaaacagtat caacgccatc cgtcactgaa tgactaccgc cgcctgatcc
    58141 cctcgctaat gtgaaggaga cgccatgaaa gagaaacctg ctgttgaggt tcgactggat
    58201 aaatggctat gggctgcccg tttttataaa acccgcgcgc tggcccgtga aatgattgaa
    58261 ggcggtaagg tgcattacaa cgggcagcgc agcaagccga gcaaaatcgt cgagctgaat
    58321 gccacgctca ctctgcgcca gggaaatgac gaacgcacgg tgattgtaaa ggcgattact
    58381 gaacagcgtc gccccgccag cgaggcagcc ttgctgtatg aagagactgc ggaaagtgta
    58441 gagaaacgcg aaaaaatggc gatggcacgt aaacttaatg ccttaaccat gccgcacccg
    58501 gaccgacgcc cggacaaaaa agagcgccgc gacctgttac gatttaaaca cggcgacagt
    58561 gaataactgt cacctgcaag agagatgatt atgccgcaac atgaccaatt acatcgctat
    58621 ctgtttgaaa attttgccgt tcgcggcgaa ctggtaaccg tttcggaaac cctgcaacag
    58681 atccttgaga accacgatta tccgcagccc gttaaaaacg tgctggcaga actgctggtt
    58741 gcgaccagcc tgttaaccgc tacgctgaag tttgatggtg atatcaccgt acagctgcag
    58801 ggcgacggtc cgatgaatct ggcggttatt aacggtaaca ataaccagca aatgcgcggt
    58861 gtggcgcgcg tgcagggcga aattccagaa aatgccgacc tgaaaacgct ggtcggcaat
    58921 ggttacgtgg tgatcaccat taccccgagc gaaggcgaac gctatcaggg cgtagttggt
    58981 ctggaaggtg ataccctggc ggcctgccta gaagattact ttatgcgttc tgaacagctg
    59041 ccgacgcgcc tgtttattcg caccggcgac gtagacggca aaccggctgc aggcggtatg
    59101 ttgttgcagg taatgcctgc gcaaaatacc caacaggacg actttgacca cctggcgacg
    59161 ctaaccgaaa ccatcaaaac cgaagaactg ctgaccttac cggcaaacga agtgttgtgg
    59221 cgtttgtatc acgaagaaga ggtgacggtt tacgatccgc aggatgtgga gttcaaatgc
    59281 acctgctcgc gtgaacgttg cgccgatgcg ctgaaaacgc tgcctgatga agaagttgat
    59341 agcatcctgg cggaagatgg cgaaattgac atgcattgtg attactgcgg taaccactat
    59401 ctgttcaatg cgatggatat tgctgaaatc cgcaacaacg cgtctccggc agatccgtaa
    59461 gttcattaat gagtatgtcc ggcagagagg gctctgccgg attttacgac ttaatggcct
    59521 gatgtgctgc gcttaccagc gcctatgaaa taaatcgcaa attattttgg tcagatacag
    59581 cgtcgtatcc gataaaagcg ttgtcactta cttaagttta tttccagcgt ttcaccatct
    59641 accgtcagat cgcgccacgc tttcggcctc gtccccagtt ttccatcctt cgcagctgtg
    59701 agtaattttt ctttcgttat ttcgttcgga agtaaactca tatcaagcgc gtcccattct
    59761 tttctgtctg agtcaaagcc atagacaatg actctgtcat cattaaaagc aaacaggatc
    59821 cgctccgcct gaccatcact attcagatct tgctcgacca gtacgcaggc gtctttttca
    59881 atacaggtca tcacgttata gcgatcctgg attaaggccg accagaatgt cgcatccggt
    59941 ttaacagaac caggggcaat taacacattt tcggcgaata ctttttccga tacctgttcc
    60001 tgaagatgtt gctctccatc taacgccatc agcagatccc gcgcgcgttt cgggtctttc
    60061 ataaacccgg catcgctttt cagcgactca agcgccgcac gtccatagcg accgctctgt
    60121 tcgagcatgt agatagttac ctggtctggc gtgtttttgc cgctctgata acgcgccata
    60181 tgactgttca cgctaatacg catactgtcc agcaccggcg aattaagaag caccagtatc
    60241 accaacacca ataacgaaac cgcaaggttc actttgccct gaagaacaag gggattttgc
    60301 ccattacgcc agacgatact gacaaaatac cccagcgacc acaccagtaa caccagctcc
    60361 gccagcgcgc cctgcaagcg gtcgacagtc cagccgtact gagcgacccg cagccataac
    60421 ccccaggtgg cgacgaacac atacagcgga gcaaccagca aagcggtttt aatcaggcaa
    60481 cgcaacggcc ccgtccaggg aagtgacgct ttttgcggat cgcggacaat agccattaag
    60541 atcaattgca aaaaggccag cgttaacagc aacccggcgg cggagatgtg gcgagaaatc
    60601 gcgctcaggc ccgtaaacgg cagggtgatg ataaacatca gggttagcaa tgatactaac
    60661 ggcagcaacc ccgtggcgat tagcgtgaac aacttttgaa tagagtcgat taaacgtgac
    60721 tgtgtacgcg cgaggatcac cgccagtgcg gtaaccagac caaatgtaag atacataaac
    60781 cagtcggttg caaaaaagag cgtattgaaa aacgtgatgc caacaagttt aaacaactca
    60841 ccccacagta aaagcaccag ccacgtcagg ccattggaga gaaaaatcac cagtaatata
    60901 agtacgttat gccatactga ctggtaaaag tagctatagc ggctactgcc attgcggata
    60961 cgaaggcttt gttgtatcca cggtagcaac aacattgcca tcaacagcag atagcagcca
    61021 aaatcccaaa tcgccttttc agctctccag gggttcatgc catcagtttg ccacttcagc
    61081 caaccgctca tccctgtcgt ggcaataaac accagcgcca gccatcccca aaggcgcttc
    61141 tgtttaaaag agatcacgga gaagagtaaa acggatgaaa acgcgacggt cgcaggcacg
    61201 ccataaacaa tccaactgtg attttttccg gcaagccagg cgataagcag gtagcaaacc
    61261 agcccctgaa gtaatccggt agcaatcatc ccctgaagta atccggtagc aatcatcccc
    61321 cagcgtgttg ccggtgaaag ttcaacgtta tccatgttgg ttatccagaa tcaaaaggtg
    61381 ggttaattat cgcatccggg cagtagtatt ttgctttttt cagaaaataa tcaaaaaaag
    61441 ttagcgtggt gaatcgatac tttaccggtt gaatttgcat caatttcatt caggaatgcg
    61501 attccactca caatattccc gccatataaa ccaagattta accttttgag aacattttcc
    61561 acacctaaaa tgctatttct gcgataatag caaccgtttc gtgacaggaa tcacggagtt
    61621 ttttgtcaaa tatgaatttc tccagatacg taaatctatg agccttgtcg cggttaacac
    61681 ccccaaaaag actttactat tcaggcaata catattggct aaggagcagt gaaatgcgcg
    61741 ttaacaatgg tttgcccccg caagaactcg aggcttatgg tatcagtgac gtacatgata
    61801 tcgtttacaa cccaagctac gacctgctgt atcaggaaga gctcgatccg agcctgacag
    61861 gttatgagcg cggggtgtta actaatctgg gtgccgttgc cgtcgatacc gggatcttca
    61921 ccggtcgttc accaaaagat aagtatatcg tccgtgacga taccactcgc gatactttct
    61981 ggtgggcaga caaaggcaaa ggtaagaacg acaacaaacc tctctctccg gaaacctggc
    62041 agcatctgaa aggcctggtg accaggcagc tttccggcaa acgtctgttc gttgtcgacg
    62101 ctttctgtgg tgcgaacccg gatactcgtc tttccgtccg tttcatcacc gaagtggcct
    62161 ggcaggcgca ttttgtcaaa aacatgttta ttcgcccgag cgatgaagag ctggcaggtt
    62221 tcaaaccaga ctttatcgtt atgaacggcg cgaagtgcac taacccgcag tggaaagaac
    62281 agggtctgaa ctccgaaaac ttcgtggcgt ttaacctgac cgagcgcatg cagctgattg
    62341 gcggcacctg gtacggcggc gaaatgaaga aagggatgtt ctcgatgatg aactacctgc
    62401 tgccgctgaa aggtatcgct tctatgcact gctccgccaa cgttggcgag aaaggcgatg
    62461 ttgcggtgtt cttcggcctt tccggcaccg gtaaaaccac cctttccacc gacccgaaac
    62521 gccgcctgat tggcgatgac gaacacggct gggacgatga cggcgtgttt aatttcgaag
    62581 gcggctgcta cgcaaaaact atcaagctgt cgaaagaagc ggaacctgaa atctacaacg
    62641 ctatccgtcg tgatgcgctg ctggaaaacg tcaccgtgcg tgaagatggc actatcgact
    62701 ttgatgatgg ttcaaaaacc gagaacaccc gcgtttctta tccaatctat cacatcgata
    62761 acatcgttaa gccggtttcc aaagcgggcc acgcgactaa ggttatcttc ctgactgctg
    62821 atgctttcgg cgtgttgccg ccggtttctc gcctgactgc cgatcaaacc cagtatcact
    62881 tcctctctgg cttcaccgcc aaactggccg gtactgagcg tggcatcacc gaaccgacgc
    62941 caaccttctc cgcttgcttc ggcgcggcgt tcctgtcgct gcacccgact cagtacgcag
    63001 aagtgctggt gaaacgtatg caggcggcgg gcgcgcaggc ttatctggtt aacactggct
    63061 ggaacggcac tggcaaacgt atctcgatta aagatacccg cgccattatc gacgccatcc
    63121 tcaacggttc gctggataat gcagaaacct tcactctgcc gatgtttaac ctggcgatcc
    63181 caaccgaact gccgggcgta gacacgaaga ttctcgatcc gcgtaacacc tacgcttctc
    63241 cggaacagtg gcaggaaaaa gccgaaaccc tggcgaaact gtttatcgac aacttcgata
    63301 aatacaccga cacccctgcg ggtgccgcgc tggtagcggc tggtccgaaa ctgtaatgat
    63361 ttgaagctgg agaatatcta tccagtatct tatagaaagc aaaacgggag gcaccttcgc
    63421 ctcccgttta tttacccttc ttttgtcgtg ccctgcgccc gcgttaccgg cactggcagc
    63481 caggcgcgaa tggaaagccc gccccgctcg ctggtgccaa gctccagcat cccgttatgg
    63541 ttatccacga tacgctgcac aattgccagc cctaatcccg tgccgctaat ggtgcgcgca
    63601 ctgtcgccgc ggacaaacgg ctggaacagg tgcttacgtt gttccggcgc aattcccgga
    63661 ccgtcatctt ccacctggaa ccaggcgcga ttcggctccg ttccgctgct gactttgatc
    63721 cagccattgc cataacgggc ggcgttgacc accatattcg ccaccgcgcg tttgatcgac
    63781 agcgggtgca ttttcacttc aatgctgccg gggtaaagcg cggtttcaat ttcccgctca
    63841 tagccacttt cggcagcaat cacctcaccg agcactgcat taagatccgc catttccatc
    63901 ggcatctcct gcccggtgcg caggtagtcg ataaactgct caatgatggc gttgcactct
    63961 tcgatatctt tattgatcga ttctgccaga tagccatcct gctcgctcat catctcagtc
    64021 gccaggcgaa tacgcgtcag cggcgtgcgc aagtcgtgac ttacccccgc catcagcagc
    64081 gtgcggtcat ccgccagttg cttaacacca gccgccatat ggttaaaggc acgggtaacg
    64141 gaacgcacct ccgaagcgcc atattcacgc agcggcggcg gaataatccc tttaccaacc
    64201 tgcaaggctg cgtgttcgag atcgaccaac ggtcggttct ggatacgaat aaacagccac
    64261 gccccgccta tcgccaatag cataatcgcc agcgtatagc ggaacagcgg agagaaatcg
    64321 ccctgatgaa tttcggtcag cggcacgcgt acccagatat tgggcgacag ccaggttttc
    64381 agccagacga caggcgaact tttgttgacc tcaacgcgca cttccgtcgg gccgcccagt
    64441 tgctgtgcca tctgatggct taagaattca tagtgttgcg cccaacgcag acctgcctct
    64501 tcggcagcct cgttggagta gagagagatc cccagctcac ggtagatctc ccgacggaaa
    64561 gcgggaggca caaccaactg cgtgccgtcc tccagttgca gtttgtcggt catcaacata
    64621 cgcacttcgt acgcgaggac tttattaaac tgctggaggc tcggcaaaat cgcgaagttc
    64681 agcaccacca gataagtcgt caccaggctg gcgaacagca aggtgacgat gagcaataac
    64741 gtacgggcaa atgaacttcg tggcgagaag cgcaatcgcc tcatgcttta gagccgtccg
    64801 gtacaaagac gtagcccagg ccccaaacgg tctgaatgta acgcggatgc gctggatctt
    64861 cttctaccat gcggcgcaga cgcgaaatct gcacgtcgat ggagcgttcc attgcggaat
    64921 attcacgacc acgggcaagg ttcatcagct tatcgcggga gagcggctca cgcggatggc
    64981 tgaccagtgc cttcagtacc gcaaactcac cgctggtgag cggcatcggc tcatcttcgc
    65041 ggaacatttc gcgcgtaccg aggttaagtt tgaacttacc gaaagcaatt accgcctctt
    65101 cctgtgacgg tgcgcctggc agttcgttcg cctgacgacg cagcaccgca cgaatacggg
    65161 ccagcagttc acgcgggtta aacggttttg gaatgtagtc gtcagcgcca atctccaggc
    65221 ctacgatacg gtccacttct tcccctttcg ccgtcaccat aatgatcggc atcgggttgc
    65281 tctgactacg aagacgtcgg caaatcgaca agccatcttc accaggtaac attaaatcca
    65341 gtaccataag atggaaagat tcacgagtca gcaggcgatc catctgttct gcattagcga
    65401 cgcttcgaac ttggaagcct tgttcggtga gataacgttc cagcagcgca cgcaggcgca
    65461 tgtcgtcatc gaccaccaga atcttgtagt tctcttgcat tgtttgtact cccaaaggtt
    65521 cgcaacaatt tgtaagcgtg tattcttaaa aaagctcacg ttcgtcacca gctaaatctg
    65581 gtatgaattt cagcctaaat tgttacaaag catattaaac agcagcttaa gtatacaatt
    65641 tattcggcga aacattattg attctgttga tatgatcacg ttatacccaa tgtgcgcatt
    65701 atcaaacaga caaagggaat caacgagatg aaaacgcccc tggttacccg ggaagggtat
    65761 gaaaaactca aacaagagct taattatctc tggcgtgaag aacgcccgga ggtcacaaaa
    65821 aaggtgacct gggccgcaag tctgggcgac cgcagcgaaa atgctgacta tcagtataat
    65881 aaaaagcgtc tgcgtgaaat cgaccgtcgc gtgcgctatc tcactaaatg cctggaaaat
    65941 ctcaaaatcg tcgattactc ccctcagcag gaaggcaaag tcttttttgg cgcgtgggtg
    66001 gagattgaaa acgacgatgg cgtgactcac cgtttccgta ttgtcggcta cgatgaaatt
    66061 tttggccgta aagattacat ctctatcgat tccccgatgg cccgcgcatt gctgaaaaaa
    66121 gaagtcggcg atctggcggt ggtgaatacc cctgccgggg aagcgaactg gtatgttaat
    66181 gctatcgagt acgtgaaacc gtaaggaaga gtcttaacct cctgccgatg gctggcattt
    66241 ttgccagcca gtccgtataa ctatcccctg atttttgatc cgaaaagatg aactcaaacc
    66301 atgatgaatg attcgttctg ccgcattatt gcgggtgaaa ttcaggcgcg cccggaacag
    66361 gttgacgctg ccgttcgcct gcttgacgaa gggaataccg tgccgtttat cgcacgttat
    66421 cgtaaggaaa tcaccggcgg tctggatgac acgcagctgc gtaatctgga aacgcgtctg
    66481 agctatctgc gcgagctgga agagagacgt caggcgatcc tcaaatccat ttccgagcaa
    66541 ggcaaactca ccgatgatct ggcgaaggcc atcaacgcca ccctaagcaa aaccgaactc
    66601 gaagacctct acctgcccta caaacctaaa cgccgcaccc gcgggcaaat cgccattgaa
    66661 gcagggcttg agccgttggc tgacctgctg tggagcgatc cgtcacacac gccagaagtc
    66721 gccgctgcac aatatgttga tgccgataaa ggcgtggcag ataccaaagc cgcgctggat
    66781 ggcgcgcgct atatcctgat ggaacggttt gccgaagatg ccgcgctgct ggcgaaagtg
    66841 cgtgattatc tgtggaagaa cgcgcatttg gtttctacgg tggtgagcgg taaagaagag
    66901 gaaggggcga aattccgcga ctatttcgat catcacgaac cgttgtccac ggtgccttct
    66961 caccgcgcgc tggcgatgtt ccgtgggcgt aacgaaggcg tactccagct ttcgctgaat
    67021 gccgatccgc agttcgatga gccgcccaaa gagagctatt gcgagcaaat catcatggat
    67081 caccttggcc tgcgcctgaa caatgccccg gcggatagct ggcgcaaaag cgtagtgagc
    67141 tggacctggc gcatcaaggt gctgatgcat ctggaaaccg aactgatggg taccgtgcgc
    67201 gaacgcgcgg aagatgaagc aatcaacgtc tttgcccgta acctgcacga tctgctgatg
    67261 gcggcccctg ccggactgcg tgcaacgatg ggcctcgatc cgggtctgcg taccggggta
    67321 aaagtggcgg tggtcgatgc cactggcaaa ctggtggcga ccgataccat ttacccgcac
    67381 accggacaag ccgcaaaagc agcgatgacc gttgctgcct tgtgtgaaaa acataacgtt
    67441 gaactggtgg cgatcggcaa cggtacagct tcccgcgaaa ctgaacgttt ctatctcgac
    67501 gtgcagaagc agttcccgaa agtgaccgca cagaaagtga tcgtcagcga agctggcgcg
    67561 tcggtttact cagcttccga gctggcagcg caggagttcc cggatctcga cgtttcgctg
    67621 cgtggtgcgg tatctatcgc ccgccgtttg caggatccgc tggcggagct ggtgaaaatc
    67681 gatccgaaat ctatcggcgt aggtcagtat cagcatgacg tcagccagac gcaactggcc
    67741 cgcaaactgg atgcagtagt agaagactgc gtaaacgccg ttggcgtcga tctcaacacc
    67801 gcttctgttc cgctattaac ccgcgtggcg ggcctgacgc gcatgatggc gcaaaacatc
    67861 gtggcctggc gcgatgagaa cggccagttc cagaaccgtc agcaactgct gaaagtcagc
    67921 cgtctggggc cgaaagcctt cgagcagtgc gcgggcttcc tgcgcattaa ccacggtgat
    67981 aacccgctgg acgcctctac cgttcatccg gaagcctatc cggtggtgga acgcattctg
    68041 gcagcaacac agcaggcact gaaagatctg atgggtaaca gcagcgaact gcgtaacctg
    68101 aaagcgtctg actttactga tgaaaaattc ggtgtgccga cagtaactga catcatcaaa
    68161 gagctggaaa aaccgggtcg cgatccgcgt ccggaattta aaaccgctca gtttgccgat
    68221 ggcgtcgaga caatgaacga cctgcaaccg ggtatgatcc tcgaaggcgc agtgaccaac
    68281 gtcaccaact ttggcgcgtt tgtcgatatt ggcgtgcatc aggacggcct ggttcacatc
    68341 tcttcattgt cgaacaagtt tgtggaagat ccgcataccg tggtgaaagc gggcgacatt
    68401 gtgaaggtga aagtgctgga agtggatctt cagcgtaaac gtatcgccct gactatgcgt
    68461 ctggacgagc agcctggcga aaccaactct cgtcgcggcg gcggtaatga acgcccgcaa
    68521 aacaaccgcc cggcagccaa accacgcggt cgtgaagcgc agcctgccgg taatagcgcg
    68581 atgatggatg cgctggcggc agcgatgggt aaaaaacgtt aaacgcccgg actggcccct
    68641 aaacctgcgc gataaggcgt tcacgccgca tctggcaacg aacgctttgc ctgacataaa
    68701 agtgccggag aatatctccg gcacttttat tccacagcca aactcacaat atactccgtt
    68761 aatatttatc acttcattaa caactgaaac cttaattaaa cattagccag tccgggtaac
    68821 tcactattcg aattatattt tcgctgcgat ataaccttga gccacatcaa cattgagtca
    68881 gattattatt caaaccaaca ttcgcacaca ttttaagtat tgctgataga aaccattctc
    68941 attatcattg tgttgttgat tatttaatct ctcctttgtt ggcaaatcat ctggtctcat
    69001 gtcgcggtca aacgccccat gaggtagtta tccagttaat gagaaacaag taggcaccta
    69061 tgcaatacac tccagatact gcgtggaaaa tcactggctt ttcccgtgaa atcagcccgg
    69121 catatcgcca aaaactgctt tctctaggca tgttacctgg ctcctctttt gatgtggtgc
    69181 gcgtcgctcc actcggcgac cccattcata tcgaaacccg tcgtgtgagc ctggtattac
    69241 gcaaaaaaga tctggcctta ttagaagtgg aagcggtttc ctgttaatac ggtgataaca
    69301 acaatgaaaa aattaaccat tggcttaatt ggtaatccaa attctggcaa gacaacctta
    69361 tttaaccagc tcactggcgc acgtcagcgt gtaggtaact gggctggcgt taccgtcgaa
    69421 cgtaaagaag ggcaattctc caccaccgat catcaggtca cgctggtgga cctgcccggc
    69481 acctattctc tgaccaccat ctcatcgcag acctcgctcg atgagcaaat cgcctgtcac
    69541 tacattttga gtggcgacgc cgacctgctg attaacgtgg tggatgcgtc taaccttgag
    69601 cgtaacctgt acctgacgct gcaactgctg gaactcggca ttccctgcat cgtggcgctg
    69661 aacatgctcg acattgccga gaagcaaaat attcgtattg aaattgatgc tctatcggca
    69721 cgtcttggct gcccggtgat cccgctggtt tcaacccgtg gtcgcggtat tgaagcgctc
    69781 aagctggcga ttgatcgcta taaagctaac gagaatgtgg aactggtgca ttacgcacag
    69841 ccgctactca acgaagcaga ttcactggca aaagtgatgc cttccgacat cccgctgaaa
    69901 caacgtcgct ggctgggcct gcaaatgctg gaaggcgata tctacagccg cgcctacgcc
    69961 ggtgaagcgt cgcagcatct ggatgccacc ctcgcccgtc tgcgtaatga aatggatgat
    70021 ccggcgctgc acattgccga tgcgcgttac cagtgcattg ctgccatctg tgatgtggta
    70081 agcaacaccc tgacggcaga acccagccgt ttcaccactg cggtagataa aatcgtgctc
    70141 aaccgtttcc tcggtctgcc gattttcctc tttgtgatgt acctgatgtt cctgctggct
    70201 atcaacatcg gcggggcgtt acagccgctg tttaacgtcg gctccgtggc gctatttgtg
    70261 catggtattc aatggattgg ctacacgctc cacttcccgg actggctgac tatcttcctc
    70321 gcccagggcc tgggtggcgg tattaacacc gtgctgccac tggtgccgca gatcggcatg
    70381 atgtacctgt tcctctcctt ccttgaggac tccggctata tggcgcgtgc agcgtttgtg
    70441 atggaccgcc tgatgcaggc gctgggcttg ccggggaaat cctttgtgcc gctgatcgtc
    70501 ggtttcggtt gtaacgtacc gtcggtaatg ggcgcacgta cgcttgatgc accgcgtgaa
    70561 cgtctgatga ccatcatgat ggcaccgttt atgtcctgcg gcgcgcgtct ggctatcttc
    70621 gcagtattcg cggctgcctt cttcgggcag aacggtgccc tggcggtttt ctcgctgtat
    70681 atgctcggta tcgtgatggc ggtgctgact ggcctgatgc tcaagtacac catcatgcgc
    70741 ggtgaagcga cgccgtttgt catggagctg ccggtctacc atgtaccaca cgttaaaagc
    70801 ctgattatcc agacctggca gcgtctgaag ggcttcgttc tgcgtgctgg taaagtgatt
    70861 atcatcgtca gcattttcct gagcgctttc aacagcttct cgttgagcgg gaaaatcgtc
    70921 gataacatca acgactcggc gctggcttcc gtcagccggg tgataacccc tgtcttcaag
    70981 ccgattggcg tacatgaaga taactggcag gcaacggttg gcctgtttac gggcgcaatg
    71041 gcaaaagaag tggtggtggg tacgctcaac accctctaca ccgcagaaaa tattcaggac
    71101 gaagagttca atccggcgga attcaacctc ggtgaagagc tgttcagtgc ggtagatgaa
    71161 acctggcaga gcctgaaaga caccttcagc cttagcgtac tgatgaaccc cattgaagct
    71221 agcaaaggca acggtgaaat gggtaccggg gcgatgggcg tgatggatca gaaattcggt
    71281 agcgcagctg ccgcatacag ctacctgatt ttcgtcctgc tgtatgtacc atgtatctcg
    71341 gtgatgggag ccatcgcccg tgaatcaagc cgtggctgga tgggcttctc catcctgtgg
    71401 gggctgaata tcgcttactc actggcaaca ttgttctatc aggtcgccag ctacagtcag
    71461 cacccaactt acagcctggt gtgcattctg gcggtaatcc tgtttaacat cgtagctatc
    71521 ggtctgctgc gccgcgcgcg tagccgggtg gatatcgaac tgctggcaac ccgcaagtcg
    71581 gtaagcagtt gctgcgcagc cagcaccacc ggtgattgcc attaatggct tcacttattc
    71641 aggtacgtga tttgctggcg ttacggggcc gtatggaagc gacccagata agccagacat
    71701 tgaacactcc gcagccaatg attaacgcca tgctgcaaca gctggaaagc atgggcaaag
    71761 ccgtgcggat tcaggaagaa cctgacggct gcctctctgg cagttgtaaa agctgcccgg
    71821 aaggcaaagc ctgtctgcgc gagtggtggg cgctgcgtta accatactcc atcgcctgtt
    71881 ttgaaaagcc cggtatgcgt ttgcatccgg gcttttttgc gtgcggcttt ccataaaaat
    71941 gcaattcttg cagcgcaacg taagttcctc tgaaagcgtc tcgcagggat gaaaactcgc
    72001 taatgcacag atgtggagtg gcgcgtagag tcgcggcatt caaacaacag gtgaaggaac
    72061 gccatgagta aaaagcagag ttccacccca catgatgcgc tgttcaaact ctttttacgc
    72121 caaccggaga cggcacgcga ttttcttgcg tttcatttac cggcacccat tcacgcgctc
    72181 tgtgatatga aaaccctcaa gctggagtcg aggagcttta ttgatgacga tctgcgtgaa
    72241 agctattccg atgtgctgtg gtcggtgaaa acggaacaag gaccaggata catctattgt
    72301 ctgattgaac atcaaagcac ctcaaacaaa ctgatcgcat ttcgcatgat gcgttacgct
    72361 attgccgcaa tgcaaaatca ccttgatgct ggatacaaaa cgttgccgat ggtggtgcca
    72421 ttgttgtttt accacggtat tgaaagcccc tatccctatt cgctgtgttg gctggattgt
    72481 ttcgccgatc ccaaactggc aaggcagctt tatgcctccg catttccgct gattgatgtc
    72541 accgtcatgc ctgatgatga aatcatgcag caccgacgta tggcgctgct ggagttaatt
    72601 caaaaacata ttcgtcaacg cgatctgatg gggctggtag agcaaatggc ctgcttatta
    72661 agtagtggat acgctaatga cagacaaatc aaagggctgt ttaattacat actgcaaact
    72721 ggcgacgctg tacgttttaa cgattttatc gacggcgttg ccgaacgttc accgaaacac
    72781 aaggagagtt taatgactat tgcggaaaga ttgcggcagg agggggaaca atccaaagcc
    72841 ctgcatatag ccaaaataat gcttgaatcc ggagtccctc ttgcagacat catgcgattt
    72901 accgggctgt cagaagaaga gttggctgcg gcgagtagac tggccccctg aatctccaga
    72961 caaccagtat cacttaa
//
LOCUS       DAETQL010000005        72058 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.005, whole genome shotgun sequence.
ACCESSION   DAETQL010000005 DAETQL010000000
VERSION     DAETQL010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.005"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1333..2919)
                     /locus_tag="K0W65_000350"
     CDS             complement(1333..2919)
                     /locus_tag="K0W65_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HBI3093757.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(3274..5445)
                     /gene="glcB"
                     /locus_tag="K0W65_000351"
     CDS             complement(3274..5445)
                     /gene="glcB"
                     /locus_tag="K0W65_000351"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HBI3093758.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5467..5871)
                     /locus_tag="K0W65_000352"
     CDS             complement(5467..5871)
                     /locus_tag="K0W65_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HBI3093759.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5876..7095)
                     /gene="glcF"
                     /locus_tag="K0W65_000353"
                     /pseudo
     CDS             complement(5876..7095)
                     /gene="glcF"
                     /locus_tag="K0W65_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(7106..8158)
                     /gene="glcE"
                     /locus_tag="K0W65_000354"
     CDS             complement(7106..8158)
                     /gene="glcE"
                     /locus_tag="K0W65_000354"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HBI3093760.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8158..9657)
                     /gene="glcD"
                     /locus_tag="K0W65_000355"
                     /pseudo
     CDS             complement(8158..9657)
                     /gene="glcD"
                     /locus_tag="K0W65_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9908..10678
                     /gene="glcC"
                     /locus_tag="K0W65_000356"
     CDS             9908..10678
                     /gene="glcC"
                     /locus_tag="K0W65_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HBI3093761.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10802..11943)
                     /locus_tag="K0W65_000357"
                     /pseudo
     CDS             complement(10802..11943)
                     /locus_tag="K0W65_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12305..14035
                     /locus_tag="K0W65_000358"
     CDS             12305..14035
                     /locus_tag="K0W65_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HBI3093762.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAAQSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            14032..14946
                     /locus_tag="K0W65_000359"
     CDS             14032..14946
                     /locus_tag="K0W65_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HBI3093763.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14979..15227
                     /locus_tag="K0W65_000360"
     CDS             14979..15227
                     /locus_tag="K0W65_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBI3093764.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            15227..16398
                     /locus_tag="K0W65_000361"
                     /pseudo
     CDS             15227..16398
                     /locus_tag="K0W65_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(16434..17513)
                     /gene="lptG"
                     /locus_tag="K0W65_000362"
     CDS             complement(16434..17513)
                     /gene="lptG"
                     /locus_tag="K0W65_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBI3093765.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17510..18580)
                     /gene="lptF"
                     /locus_tag="K0W65_000363"
     CDS             complement(17510..18580)
                     /gene="lptF"
                     /locus_tag="K0W65_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBI3093766.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18611..19171)
                     /locus_tag="K0W65_000364"
     CDS             complement(18611..19171)
                     /locus_tag="K0W65_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBI3093767.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19183..20007)
                     /locus_tag="K0W65_000365"
     CDS             complement(19183..20007)
                     /locus_tag="K0W65_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093768.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20007..21356)
                     /locus_tag="K0W65_000366"
     CDS             complement(20007..21356)
                     /locus_tag="K0W65_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBI3093769.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21402..22160)
                     /locus_tag="K0W65_000367"
     CDS             complement(21402..22160)
                     /locus_tag="K0W65_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093770.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22192..22905)
                     /locus_tag="K0W65_000368"
     CDS             complement(22192..22905)
                     /locus_tag="K0W65_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBI3093771.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23079..23771
                     /locus_tag="K0W65_000369"
     CDS             23079..23771
                     /locus_tag="K0W65_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBI3093772.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23820..25319)
                     /gene="pitB"
                     /locus_tag="K0W65_000370"
     CDS             complement(23820..25319)
                     /gene="pitB"
                     /locus_tag="K0W65_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBI3093773.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25611..27470)
                     /gene="gss"
                     /locus_tag="K0W65_000371"
     CDS             complement(25611..27470)
                     /gene="gss"
                     /locus_tag="K0W65_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBI3093774.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27675..28541
                     /gene="yghU"
                     /locus_tag="K0W65_000372"
     CDS             27675..28541
                     /gene="yghU"
                     /locus_tag="K0W65_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBI3093775.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28664..28912)
                     /gene="hybG"
                     /locus_tag="K0W65_000373"
     CDS             complement(28664..28912)
                     /gene="hybG"
                     /locus_tag="K0W65_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBI3093776.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29046..29387)
                     /gene="hypA"
                     /locus_tag="K0W65_000374"
     CDS             complement(29046..29387)
                     /gene="hypA"
                     /locus_tag="K0W65_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBI3093777.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29380..29868)
                     /gene="hybE"
                     /locus_tag="K0W65_000375"
     CDS             complement(29380..29868)
                     /gene="hybE"
                     /locus_tag="K0W65_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBI3093778.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29861..30355)
                     /locus_tag="K0W65_000376"
     CDS             complement(29861..30355)
                     /locus_tag="K0W65_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBI3093779.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30355..32058)
                     /gene="hybC"
                     /locus_tag="K0W65_000377"
     CDS             complement(30355..32058)
                     /gene="hybC"
                     /locus_tag="K0W65_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBI3093780.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32055..33233)
                     /gene="hybB"
                     /locus_tag="K0W65_000378"
     CDS             complement(32055..33233)
                     /gene="hybB"
                     /locus_tag="K0W65_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBI3093781.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33223..34209)
                     /gene="hybA"
                     /locus_tag="K0W65_000379"
     CDS             complement(33223..34209)
                     /gene="hybA"
                     /locus_tag="K0W65_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBI3093782.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34212..35330)
                     /gene="hybO"
                     /locus_tag="K0W65_000380"
     CDS             complement(34212..35330)
                     /gene="hybO"
                     /locus_tag="K0W65_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBI3093783.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35519..35806)
                     /locus_tag="K0W65_000381"
     CDS             complement(35519..35806)
                     /locus_tag="K0W65_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBI3093784.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35925..36812)
                     /locus_tag="K0W65_000382"
     CDS             complement(35925..36812)
                     /locus_tag="K0W65_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBI3093785.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36969..38009
                     /gene="gpr"
                     /locus_tag="K0W65_000383"
     CDS             36969..38009
                     /gene="gpr"
                     /locus_tag="K0W65_000383"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBI3093786.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38049..38543)
                     /locus_tag="K0W65_000384"
     CDS             complement(38049..38543)
                     /locus_tag="K0W65_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBI3093787.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38734..39618
                     /gene="yghA"
                     /locus_tag="K0W65_000385"
     CDS             38734..39618
                     /gene="yghA"
                     /locus_tag="K0W65_000385"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBI3093788.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPSIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39890..40315)
                     /gene="exbD"
                     /locus_tag="K0W65_000386"
     CDS             complement(39890..40315)
                     /gene="exbD"
                     /locus_tag="K0W65_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBI3093789.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDLVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40322..41056)
                     /gene="exbB"
                     /locus_tag="K0W65_000387"
     CDS             complement(40322..41056)
                     /gene="exbB"
                     /locus_tag="K0W65_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBI3093790.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41308..42495
                     /gene="metC"
                     /locus_tag="K0W65_000388"
     CDS             41308..42495
                     /gene="metC"
                     /locus_tag="K0W65_000388"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBI3093791.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42635..43294
                     /locus_tag="K0W65_000389"
     CDS             42635..43294
                     /locus_tag="K0W65_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI3093792.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43334..44233)
                     /gene="yqhC"
                     /locus_tag="K0W65_000390"
     CDS             complement(43334..44233)
                     /gene="yqhC"
                     /locus_tag="K0W65_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HBI3093793.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            44427..45590
                     /gene="yqhD"
                     /locus_tag="K0W65_000391"
     CDS             44427..45590
                     /gene="yqhD"
                     /locus_tag="K0W65_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBI3093794.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45695..46522
                     /gene="dkgA"
                     /locus_tag="K0W65_000392"
     CDS             45695..46522
                     /gene="dkgA"
                     /locus_tag="K0W65_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBI3093795.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46722..47648
                     /locus_tag="K0W65_000393"
     CDS             46722..47648
                     /locus_tag="K0W65_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBI3093796.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47699..47956
                     /gene="yqhH"
                     /locus_tag="K0W65_000394"
     CDS             47699..47956
                     /gene="yqhH"
                     /locus_tag="K0W65_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBI3093797.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47999..50218)
                     /locus_tag="K0W65_000395"
     CDS             complement(47999..50218)
                     /locus_tag="K0W65_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBI3093798.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(50329..51741)
                     /gene="ftsP"
                     /locus_tag="K0W65_000396"
     CDS             complement(50329..51741)
                     /gene="ftsP"
                     /locus_tag="K0W65_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBI3093799.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51816..52553)
                     /gene="plsC"
                     /locus_tag="K0W65_000397"
                     /gene_synonym="parF"
     CDS             complement(51816..52553)
                     /gene="plsC"
                     /locus_tag="K0W65_000397"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBI3093800.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52787..55045)
                     /gene="parC"
                     /locus_tag="K0W65_000398"
     CDS             complement(52787..55045)
                     /gene="parC"
                     /locus_tag="K0W65_000398"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBI3093801.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55591..56073)
                     /locus_tag="K0W65_000399"
     CDS             complement(55591..56073)
                     /locus_tag="K0W65_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBI3093802.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSELIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(56126..56518)
                     /gene="ygiW"
                     /locus_tag="K0W65_000400"
     CDS             complement(56126..56518)
                     /gene="ygiW"
                     /locus_tag="K0W65_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBI3093803.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56670..57329
                     /gene="qseB"
                     /locus_tag="K0W65_000401"
     CDS             56670..57329
                     /gene="qseB"
                     /locus_tag="K0W65_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBI3093804.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            57326..58600
                     /gene="qseC"
                     /locus_tag="K0W65_000402"
     CDS             57326..58600
                     /gene="qseC"
                     /locus_tag="K0W65_000402"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBI3093805.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58646..58978)
                     /locus_tag="K0W65_000403"
     CDS             complement(58646..58978)
                     /locus_tag="K0W65_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HBI3093806.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            59297..59878
                     /gene="mdaB"
                     /locus_tag="K0W65_000404"
     CDS             59297..59878
                     /gene="mdaB"
                     /locus_tag="K0W65_000404"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBI3093807.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59909..60223
                     /locus_tag="K0W65_000405"
     CDS             59909..60223
                     /locus_tag="K0W65_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBI3093808.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(60271..62163)
                     /gene="parE"
                     /locus_tag="K0W65_000406"
     CDS             complement(60271..62163)
                     /gene="parE"
                     /locus_tag="K0W65_000406"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBI3093809.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(62192..62773)
                     /gene="yqiA"
                     /locus_tag="K0W65_000407"
     CDS             complement(62192..62773)
                     /gene="yqiA"
                     /locus_tag="K0W65_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBI3093810.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62773..63600)
                     /gene="cpdA"
                     /locus_tag="K0W65_000408"
     CDS             complement(62773..63600)
                     /gene="cpdA"
                     /locus_tag="K0W65_000408"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBI3093811.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63625..64047)
                     /locus_tag="K0W65_000409"
     CDS             complement(63625..64047)
                     /locus_tag="K0W65_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBI3093812.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(64048..64677)
                     /gene="nudF"
                     /locus_tag="K0W65_000410"
     CDS             complement(64048..64677)
                     /gene="nudF"
                     /locus_tag="K0W65_000410"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBI3093813.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64882..66363
                     /gene="tolC"
                     /locus_tag="K0W65_000411"
     CDS             64882..66363
                     /gene="tolC"
                     /locus_tag="K0W65_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBI3093814.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            66511..67182
                     /locus_tag="K0W65_000412"
     CDS             66511..67182
                     /locus_tag="K0W65_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBI3093815.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            67188..68348
                     /locus_tag="K0W65_000413"
     CDS             67188..68348
                     /locus_tag="K0W65_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBI3093816.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(68386..69174)
                     /gene="ygiD"
                     /locus_tag="K0W65_000414"
     CDS             complement(68386..69174)
                     /gene="ygiD"
                     /locus_tag="K0W65_000414"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBI3093817.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     LNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            69317..70090
                     /gene="zupT"
                     /locus_tag="K0W65_000415"
     CDS             69317..70090
                     /gene="zupT"
                     /locus_tag="K0W65_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBI3093818.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(70148..70318)
                     /locus_tag="K0W65_000416"
     CDS             complement(70148..70318)
                     /locus_tag="K0W65_000416"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093819.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70579..71232)
                     /gene="ribB"
                     /locus_tag="K0W65_000417"
     CDS             complement(70579..71232)
                     /gene="ribB"
                     /locus_tag="K0W65_000417"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBI3093820.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(71336..71484)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71606..71896
                     /gene="ubiK"
                     /locus_tag="K0W65_000418"
     CDS             71606..71896
                     /gene="ubiK"
                     /locus_tag="K0W65_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HBI3093821.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        18029 a        19162 c        17733 g        17134 t
ORIGIN      
        1 acgtaccgtt taaacaaaga caatacgtct ctttatttct cataaaaccc caatccctta
       61 cagggcttat tgaaaaattt aaaaatcaca aatttctcaa tactcgaaat gagatcacat
      121 aagaaatcaa aaagccttct catctatttt aagttttccc aacggaaaat aagagctttc
      181 attaatactg aaccacttta ctgttctatt tgttcaggta ttgcattacg gtgtgctgag
      241 atccatcggt gcatccctgt atcccgcgat gagactaaaa tagtcgtgat gtaagctcgt
      301 atcagattct tagtgaatac cacatttgtt ataattaagt gaaaagatac attgctgatt
      361 tacattattt ggtttacaaa atgtcatcac ttgcagagtt atcgctgaca gataccttta
      421 atctcttact actttcgtgt tattgcaata caaccatccg taaagcaggg ctatttttag
      481 caggaaatac tcaggtgtca acattcgaac tccaactctg aaatattcat ataagttaat
      541 gattttaatg attttaatga ttttaatgat tttaatgatt ttttcattta ttcacaccta
      601 ataaaagtat tcaaattcag atgatttttc tgctcaacag acacaaacag gcaataatac
      661 tctccaaagc gaacaatatt tggcattacg atttaatgct tcataatcac caaattattt
      721 aaccataaat tttcttattt ctaacaaaat gattggtgtg ttgttggagt taaaatacat
      781 acataatatt aacatctgcc aacatttcac tttccgcaag acggatgatt tcgaatcctc
      841 atccacgtta ccctatccca aatatcttta ttaataggca gtaacgaaca ctgttatagc
      901 aagattaact tattaaccta acggcatctt ttatgtaaca agtggagata aattgcttaa
      961 aattgatcct cgacaaatct taaaagttct cattattctg caaaatcagc tttgcgaata
     1021 atcttatgtt tttgggagtt ttgggaattt tgatctactc attgttctag tgaaaaatat
     1081 aaataagaga aaaaacgcca ttcgtcatga aagcaacaga tgaagcaatg taataacata
     1141 ctaaaattga tctcatgaaa gtgggtactt tataaccagc acagaagcca agttgtataa
     1201 atattaaaca attcgcatcg taaaacggag actacgtata aagaagccaa tcgtaaaaac
     1261 ggttccctgg cgagataaac gacagacaaa tggaaagccg ggtgatattt accttcatcc
     1321 ggctttattg atttacgaga ctaacatccc ggtaaacaca tacgcctgca gcagggtgat
     1381 aacgctggca aaaatcagac tgtgcttcac ggtgtagcgg aacagttcag attctcggcc
     1441 caccatgccc gtcgcggcgc aggccacggc gatagattgc ggggagatca tcttgccagt
     1501 tacgccgccg ctggtatttg ctgccaccag cagagtgtca gagacgttga tttgctgcgc
     1561 cgtggtcgat tgcagtgaac caaacagggc gtttgaagaa gtgtccgacc cggtaaggaa
     1621 tacgcccagc cagccgagaa acggtgagaa aaacgggaac atcacgcctg tacctgccag
     1681 taccagcgcc agcgtggtgg acatgccgga atagttggtg acgaaggcga atgccagcac
     1741 catgccaatc gacaatatcg gccacttcag actaattagc atttcggcaa agacgccaat
     1801 ccctttcttg atgcccacgc cgaggatgaa gatagagata atcgccgcaa taaaaatagc
     1861 ggtgccgcca gccgagagag ggtcgaattt aaacaccgca tccattggcg ttggttgggc
     1921 gacaatgggt gccgctttca acacttgttg atgcaaatga gggatctgga aattaatcac
     1981 cagtgaataa aacgcgccgc ccggagcaaa taacgcttta aacggcttca tggtccagat
     2041 ggtgaccagc accgttaaga ttaaaaacgg tgaccacgca cgaatgattt gccccagact
     2101 atattctgaa ggcacgggac cgccagaaga tggcttattt accaccatcg cacctgcgga
     2161 ttgccccatg ctgattgccg tttcggtatt tttcggccgc cagactttaa ggaataaagc
     2221 gagtgagacg atactcacca gcgccgaagt aatatccggc agttccggac caatatagtt
     2281 agaggtaaag aactgagtga cagcgaagct tcccccagca accagcgccg ctggccacgt
     2341 ctctttcacc cctttccagc cgtccatcat tgctaccagc cagaacggca caagaaccga
     2401 caggaacggt aactgacgtc ccgccattgc gccaatgtgg aacggatcga ttcccgttac
     2461 ctgaccggcg accagaatcg gcacgcccaa cgcaccaaac gccaccgtcg caccaaagcc
     2521 agcagctcct tccagcaacg caccaaagga gaaaccaatc agtaacacct gcaaacgctg
     2581 atcgtcggtg atggagataa ccgagctgcg gataatatcg aactgcccgc tggcaacggt
     2641 taatttatac aggaacaccg ccgcgacaat aatccacgca attggccata atccataaat
     2701 aaagccatag cccgcagcag caaatgccat atcaatcggc attttaaagg cgaatatcgc
     2761 aatcaggata gataatataa gggttattgc tccagcaaca tgtcctttca gacgtaatac
     2821 cgcgagtgca acgaagaaga atattatcgg gatcagggcg accagagcgg atagccccag
     2881 tcctcccatc ggcatataca tttgggtcca ggtaaccata ttgttcagtc tcttattatc
     2941 tttatatgct tgatatactt aaggttgtaa taagcaaaag aggactgaac tgtaaaatat
     3001 aggcgttata ctttacagca acagtacgcc gctaacgcaa ttgctacctc tggcataaca
     3061 agtatatcgg gtaagggttt ctgttccgca cacgcaggcg cagagtatcg ttaagatgtc
     3121 catattgttg ttttaggccc gctagtaatg cgctacgggt atttaatatt gttaaaccct
     3181 gataatcgct ccggttattt ccgggataaa tgtactaccg cagttactat catagccccg
     3241 acaataaaac ttgccggggc ttttttgacg ctattaatga ctttcttttt cgcgtaaacg
     3301 ccaggcgtgt aataacggtt cggtataacc gtttggctgt ttcacgccga ggaagattaa
     3361 atcgctggca gctttaaaag cacacgagtt agcgaaattc cccgccatcg gacgataagc
     3421 cggatcgcca gcattttgct gatcaaccac tttcgccata ttctccagcg acgcctgcac
     3481 ctgttctttg gtcagaatac cgtgacgtaa ccagttggcg atatgctggc tggagatacg
     3541 cagcgttgcg cggtcttcca tcagcgccac gttgtgaata tccggcactt ttgaacaacc
     3601 aatcccctgc tccacccaac gcaccacgta ccccagaatc ccctgcacgt tgttatccag
     3661 ctcttgttgg atctcttgcg ccgaccagtt agcgttttca gccaccggaa tagtcagcag
     3721 atcgtccagc agcggttcaa attcagcact gaactcggtc tgggcaatgt tggcttgcac
     3781 gctctgtacg ttggtatggt ggtagtgcag cgcatggagc gtagcagcgg ttggtgacgg
     3841 aacccaggct gtgtttgccc cggcacgcag ttggtcgccc ttctggctgt acatgtctgc
     3901 catcaggtcc ggcattgccc acatgccttt accgatttgc gctttaccgc gcagcccaca
     3961 gaacagaccg gaaagcacgt tattacgttc gtaggctttg atccacggcg tcgatttcat
     4021 ctggttttta cgcagcatcg ggccagcttc catcaccgaa tgcatttcat cgccggtacg
     4081 gtcgaggaaa ccggtattga tgaacgccac gcggttgcgc gcctgagcga tacagctacg
     4141 caagttcagc gaggtccggc gttcttcatc cataatgccc attttcaggg tattcggtgc
     4201 cataccgagc attgtctcaa tgcgggtaaa cagtttgttg gcgaacgcca cttcctgcgg
     4261 accgtgcatt ttcggtttca caatatagac gctgccagtg cgcgagtttt tctgcacttt
     4321 taaatcatag agggcaatcg cgccagtcat gacgccatca agaatacctt ccgggatttc
     4381 attgccttcg ctgtcccaaa tcacaggaat ggtcatcaaa tgacccacgt tgcggataaa
     4441 cagcaacgag cgtccgtgca gagaaatttc agagccatcg gcggcggtgt actgacgatc
     4501 gtcattcagt ttacgtacga tttgccgacc gtttttctcc attttctctt gcagagtccc
     4561 ctgcatcagg cccagcagat tgcggtacag caggatttta tcttccgcat caaccgccgc
     4621 gaccgaatct tcgcagtcga gaatggtact gatagcagct tcgacgataa catcgttgat
     4681 gtgcgccgga tcgtctttgc caatccgccc gttggcatca atttgcagtt caatatgcag
     4741 gccgttattt ttcagcaaaa tgcaggtcgg cgcagcggca tcgccacggt aaccgacaaa
     4801 ctgtgctgga gtacgtaacg tggtttcttt accatttttc aactggatgc gtaattgttt
     4861 atcgaccacc ttaaacgcca ccacatcctg atagctgccg ttttccagcg gtagtgattc
     4921 atcgaggaaa cgtcgaaccc aggcgataac ctgctcaccg cgctgcggat cgtagccact
     4981 gaccatcgcc ccttcctgcg ggatgatgtc gctgccgtat aacgcatcgt acagtgagcc
     5041 ccagcgagcg ttcgccgcgt tcagcgcgta gcgggcgttc attgccggaa ccaccagctg
     5101 tggccccgcc tgactggtga tttcgctgtc aatgcccgtg gtttccaccg tcacgcgctc
     5161 cggttgcggc accaggtagc ccagttcacg aaggaaagat ttataggccg ctttatcttt
     5221 taccggcccc ggattgctgc gatgccactc atcaagcgcc gcctgaatgc gatcgcgttc
     5281 tgccagcaac tgacgatttt ctggtgccag atcgtgaacg atctcatcaa cattgtgcca
     5341 gaatgcagca gcgtccagtt ccacgcccgg caaaacttct ccatccacaa aacgtttaaa
     5401 attggcgtca atgcgtaaac ggccctgggt tatggtttga ctcattgttt ctctcctcgt
     5461 tttcgcttat ttcgccaaca ccgctgctgc cactttcgcg acctgcgcat cctgtgctcc
     5521 ggttaaacca gaaacgccca cggcaccaat aatttgccca tcaacaacaa ccggtacgcc
     5581 gccttccagc gacgtcagta acggcgcagt cacgaacgcg gtacgtccgt tgttcaccat
     5641 ctcttcatag cccttagttt cacgacgccc cagcgcggcg gtacgcgctt tctcctggga
     5701 gatgtaagcc gcaatcggcg cgcaatcgtc catgcgactt aacgccagca gatgaccgcc
     5761 gtcatcggca acagcaatgg aaacagacca gttatttttc tgcgcctctt cctgacctgc
     5821 ggcaataatt gcactcgcca tttgctggct aagaatgact ttagttttca tttttttatt
     5881 ccttttcaag ggcttgttct acaatttcaa tccagtgacg cacagaggta cgaccggcgc
     5941 tcgccagatg cgtctggcaa ccaatgttgg cggtgacgat catttccggt ttgccgcttt
     6001 ccagcgcatt cattttgtta tcccgcagct ggcgtgccag atcgggatgc gttaacgcat
     6061 atgttcccgc tgaaccgcag cacagatggc tgtcgggaac gtccgttaag gtaaatccaa
     6121 gacgaagtaa cactttttcc acttcgccgt tcagcttttg cgcatgttgt agggtacacg
     6181 ggcagtggaa ggccagtttt ttatcgccgc gaattgccag tttttccagc ggttcctcgc
     6241 gcagaagttc gactaaatcg accgccagtt cactgacctg acgcgcttta tcggcatata
     6301 acgcatcgtt tttcagcatc tgcccatact ctttgacgaa cgcgccgcaa ccgctggcgg
     6361 tttgcaaaat tgcctcggca cctgcttcaa tcgcgggcca ccaggcatca atattattgc
     6421 gcgcccgtgc caaccctttc tcctgcgcat taagatgata gtccaccgcg ccacaacagc
     6481 ctgcttcgtt agctgacatg acgctgatcc ccagacgatc cagcactcgc gcagttgccg
     6541 cgttggtgtt gggcgaaagc gtaggctggg cgcagccttc cagcattaaa acccgacgct
     6601 tatggcgcag cggcggacgc ggtttagctt tcaccgtttc agcaggcagt tttgctctga
     6661 cctgttccgg taaaaacggt cgcagcacca gccctacctg cgtcagcgca cggaagaccg
     6721 ccggacgcgg cactacctgg cgcaatcctt cgcgcagcat tcgctccggc agtgggcgtt
     6781 tcactttctg ctcgacaata tcacgcccga tatccagcaa attgtgatag cgcacaccag
     6841 aaggacaggt ggtttcacaa ttgcggcaag tgaggcagcg atcgagatgc tcctgtgttt
     6901 taagcgtgac ttcgttgcct tccagcacct gtttaatcag atagatgcgc ccgcgcggcc
     6961 cgtccagttc atcgcccaga agctgatagg ttgggcaggt tgcggtacaa aatccgcagt
     7021 gaacacaggc gcacaggatg ctgtcggctt ccagcgcgcg ttctgccgca tctcttcggt
     7081 taatcgggtt tgcatagcct gctcctcaaa gttccgcgta catgcgaccg gggttaaaca
     7141 cgccgcaagg gtcgagctgc tgtttaagct gctggtgata gcggaataaa ggagccgata
     7201 gcggggcaaa gccaccatct ccggcactaa agcgggtcgc atgaccgcca gcgttgcggg
     7261 cgatgcgatg gatttgattg tcatcggctg tcgatttcag ccagcgtaac gccccgcccc
     7321 agtcgatcag ttgctcgccg ggtaaatcca tcatcggcgc atcgctgggt aatgaaatgc
     7381 gccataaggt acctggtaac gagaagaacg gcagttgttg ttcacgcaat tgctgccaga
     7441 actgacccac aacctcttcg ccacccagca gttcacgcgc tgcttttacc gatccttcgc
     7501 cgccctcaag gcggatccac aacgcattgt cgaagtaaca taagccacta atgggtaatg
     7561 gctggagttg ccactcggcg atttcactca tggcttcttg caggctgatt tcccgacgca
     7621 ggctcagaga ggcacgcggt cgcggtaaca ctttcattga gatttcagtg agcacgccaa
     7681 gacaaccgta gcttccggcc attaaccgtg agagatcgta tccggcaacg tttttcatca
     7741 cttcgccacc aaaacgcaga tgttttccag tgccggtaat gatgcgcgtg ccgaggacaa
     7801 aatcgcggac cgaaccgctc cacgggcgac gcggccccgc cagcccgcag gcgaccatcc
     7861 cgccccaggt ggcttcttca ccataatgcg gcggctcgca gggaagcatt tgcccggcgc
     7921 tctccagcgc cgcttcaatc gccaccagcg gcgttccggc acgagcggtt atcaccagct
     7981 cggtcgggtc gtaattaaca atgccgcgat gacaacgcac atccagcgtt tgcccggtga
     8041 ctgggcgacc taaaaaggct ttgctattgc tgccctgaat caccagcggc gttttatcgc
     8101 taattgcctg attcacctgc tccagcagcg cctgactgta atcacactcg cgtagcatca
     8161 aaagcgctcc agttcaggaa aaggtaaatg accgtgatgc acatgcatgg caccaaattc
     8221 agcacagcgg tgtagcgtgg gaatgttttt cccagggttc agcaaaccat cggggtcaaa
     8281 cgccgccttg accgcatgga aggtcgtgat ttcatcgctg ttgaactggg cgcacatttg
     8341 attgattttt tctcgcccga tgccatgttc cccactgatg ctgccgccaa cttcaacgca
     8401 gagttcgagg atcttcccgc ccagctcttc cgcgcgggca aattcaccag gttcgttggc
     8461 atcgaaaagg attaacgggt gcatgttgcc atctccggca tgaaagacgt tggcaacacg
     8521 taaatcatat tgctgcgata aacgggcaat gccttccagt acgccaggca gggcgcgacg
     8581 cgggatggtg ccatccatgc agtagtaatc cggggagata cgtcctaccg ccgggaacgc
     8641 atttttgcga ccggcccaga aacgtacgcg ctctgcttcg tcctgtgcca aacggacgtc
     8701 agtcgcgccc gctttcaaca agatgtagtt aacccgctcg cagtcttcct gtacgtcaga
     8761 ctccacgccg tccagttcgc ataacaaaat cgcttcggcg tcgacgggat aaccggcatg
     8821 aataaaatct tccgccgcgc ggatcgacag gttatccatc atctccagcc cgccggggat
     8881 aatgccattg gcgatgatgt caccaaccgc aagtccggct ttttctaccg agtcaaagct
     8941 ggctaacaga acccgcgcca cgggcggctt cggcagcagt tttaccgtca cttcggtggt
     9001 cacgccgagc ataccttccg atccggtgaa cagcgccagc aggtcaaaac caggtgaatc
     9061 cagcgcgtcc gatcccagcg tcagcgcctc gccgtccagc gtttgcactt caattttcag
     9121 caggttatgt acggtcagac catatttcag gcagtggacg ccaccggcat tttccgccac
     9181 attgccgcca attgaacagg cgatttgtga ggaaggatcc ggcgcgtaat agagattatg
     9241 cggcgcaacg gcctaggaga tcgccaggtt acatacgccc ggctgcacgc gcgcgcggcg
     9301 accaacgggg ttaatgtcga ggctctcttt aaagcgcgcc atcaccaaca acacaccttt
     9361 ttccagcggc agcgcgccac cagaaagccc ggtgcctgca ccacgggtca ccaccggtac
     9421 acgcaggcga tggcagacag ccagaatcgc tgtcacctgt tccatttgct taggcagaac
     9481 aaccagtaat ggacgcgtgc gatacgcgct caacccgtca cactcgtaag gaatgatctc
     9541 ctcatcggta tgcaggatct caagtccagg gacatgctca cgcagtgcca tcagtaccga
     9601 tgtgcggtcg acatcgggta aagcgccatc aagacgctct tcgtacaaga tgctcatgag
     9661 taggcttcgc tttgttgtgt tgtgtggcag ctgatttttg cgcgctgctt ctgtgaacag
     9721 ttattaagcg ggcttttcgt tttcgtctat ctctttagct accggtcaga ccattttttt
     9781 tccagctctg tgaccttgtc ttggttaact taatgttaaa ttgatgtaac ataatcactt
     9841 gcgtgatgtg cgtgttttgc gagttaagaa cagaaaaatt ggtcctacct gtgcacgagg
     9901 tccgggaatg aaagatgaac gtcgccctat ttgcgaagtg gttgcagaga gtatcgaacg
     9961 gttaattatc gacggcgtac tgaaggtcgg tcagccgctt ccctcggaac gtcgactgtg
    10021 tgaaaagctc ggcttctcac gctccgcact gcgtgaaggg ctgaccgtgc tgcgcgggcg
    10081 cgggattatt gaaacggcgc agggtcgcga ttctcatgtc gcacggctta atcgggagca
    10141 ggacaccagc ccgctgatcc atctgttcag tacgcagccg cgaacgctgt acgatctgct
    10201 cgacgttcgc gcattactgg agggcgaatc ggcaaggctg gcggcaacgc tgggaacgca
    10261 ggctgatttt gttgtgataa cccgctgtta tgaaaaaatg ctcgccgcca gtgagaacca
    10321 caaagagatt tcgctgatcg aacatgcgca gttggatcac gctttccatc tcgccatttg
    10381 tcaggcttct cacaatcagg tgctggtgtt tacgctgcaa tcattgaccg atctgatgtt
    10441 taattcagtg tttgccagcg taaataatct ctaccatcga ccacagcaaa aaaagcagat
    10501 cgatcgccag catgcgcgga tctacaacgc ggtgttgcag cggctgccgc acgtcgctca
    10561 gcgcgcagcg cgcgatcatg tacggaccgt gaaaaagaat ctccacgata tcgagctgga
    10621 aggccaccat ttgattcgct cggcggtgcc gctggagatg aacaaggtgg gaatgtgagc
    10681 agagttttac cctatcccca aggcgagggg accgcccctg cacttaacag gatctggtgt
    10741 taaatctgtt catctatacc ctctcccgaa tgaccgcccc tgcgctaaac aggatctggt
    10801 attaaatttg cttttcatac aatcggtaac gcttgtacgg ctccgcccca atgcgttcca
    10861 gcatgttatt catgcctgta ttggtttcga ggatccatga catctccagc gcatcgatct
    10921 tccggcgggc aaacggatcg cgtaaggctt caatcaataa cagcgcaatc accgggccga
    10981 tgcggctgaa ctgatactcg tcgcgcacgc ccatcagcgg tactcgcgca gttcgcacac
    11041 cgctgacttt caaacgccac agcaattttg cccagccgaa gggaaagagc gatccgttca
    11101 gatcggcaat cgcctcgttg atgttaggca agccacaata aacgcgcagg gtgcagaatc
    11161 aatctcagcg atgtagatca tatcgtccgg caccagatat ttaagttggt cgcccatggt
    11221 cgcgaattca tgttcggtaa acggcacaaa tccccagttg tgctgccagc cagagttgaa
    11281 aatctcacgc aggatctgca tctcttcggc aaaccgctga cgattgatgc agcgaatggt
    11341 cacctttttg cgcacctgat ccatcagttt ttttagcgcc ggagagaaag tgagatcggt
    11401 tcgctgcatc caccacgcca gtaaatcaat gcctttgtga taacccagtt gttcaatatg
    11461 cgcggcatac cacggtttgc cgtgtggcat catcgcacag ggtggtgtgt caaaaccttc
    11521 aatcagtaat ccgctttcct gattgatatt caggctgaaa ggcccgctga tcttacttgc
    11581 accttgtgac ttcaaccacg cttccgctgc gccaaacaac gcggcaaaat cctgcggatc
    11641 atcaatggcg tcaatcatgc cgaaatgacc ggtatcttca ccgtaacgct cacggtgcaa
    11701 ggtatctatt tgcgcggtaa tacgcccaac tatctgcccc gctttttttg ccacccacgc
    11761 ctgccagatg atatggtccg tccccggatt tttcgcagac aaatgctcgt tgcgttcaat
    11821 gaataaaggg ggtatccagt ttggatcgtc gggataaagt gatgacggaa aagcgataaa
    11881 tgccttaagg tcatttttat taattacttt ttcaatttta atcatcagca gatcgcattc
    11941 catatctctg tggataacaa ataattaatg gtgaaaaaca ggttgcaaca gcaacgagcg
    12001 gcatcttaat aatcaactca atgaatacaa acgacgaata aatagtatta attaatgatt
    12061 atgataaggt cagaggctac acagaaacgc ctcaatgcct tttgtcaatt taaggcgacc
    12121 ggacaaggct tcttcttaat atattcataa attaaatatc attcataata aaaacaaaaa
    12181 acttaaactc attatttaaa tcaatgttca ttttcacacc gatgaaaata ctatctcatt
    12241 tatcttttat gcctatataa tccaccgcat tattgtcatt ttatttttga tatccaattt
    12301 tttcgtggtg tatatgtcta ataaaatctt tacgcattcc ctacctatgc gctatgccga
    12361 ttttccaacg ctggttgatg ctttggacta cgccgctcag agtagagccg gaatgaattt
    12421 ttatgacaga cgttgccaac ttgaagatca actggaatat cagacattaa aaacgcgtgc
    12481 cgaagctggt gcgaagcggt tgttatcgct gaacctgaaa aaaggcgaac gcgtggcgct
    12541 gattgccgaa acgagtagcg ggtttgtaga ggcttttttt gcctgtcagt atgctggctt
    12601 agtcgccgtc ccgttggcga ttccaatggg cgtcggtcag cgggattcct ggagcgccaa
    12661 actccagggt ttactggcaa gttgccagcc cgcagccatt atcactggtg atgagtggtt
    12721 gccactggtc aatgccgcga cgcataacaa ccccgaatta catgttttaa gccacgcttg
    12781 gtttaaggca ttaccggaag ccgatgttgc gctccagcgt ccagttccga acgatatcgc
    12841 ctacctccag tacacctccg gcagcacccg ttttccccgt ggcgtcatta tcacccatcg
    12901 cgaagtgatg gctaatctac gtgctataag ccacgatggc attaaattac gccctagcga
    12961 ccgctgcgtc tcctggcagc ctttctacca tgatatggga ctggtcggct ttctcctgcc
    13021 ccccgtcgcc acgcaacttt cagtagatta tttgcgcact caggattttg ccatgcgtcc
    13081 tttgcaatgg cttaaattga tcagtaaaaa tcgcggcacc gtttccgttg cgccgccgtt
    13141 tggctatgaa ttgtgccaac gccgcgtgaa tgaaaaagat ctcgctgaac tggatctttc
    13201 ctgctggcgc gtcgctggta ttggtgctga gccgatctcc gcagaacaac tccatcaatt
    13261 cgctgaatgt ttccgtcagg ttaactttga caataaaact ttcatgccgt gctacggact
    13321 ggcagaaaat gcgctggctg tcagcttctc tgatgaagcc tccggggttg tggttaacga
    13381 agtggatcgc gacatcctcg aatatcaggg taaagccgtc gcgccgggtg cagagacacg
    13441 cgccgtatcg actttcgtca actgcggcaa agcgttgccg gaacatggta ttgaaatccg
    13501 caatgaagca ggtatgccgg tcgcggaacg tgtggtaggc catatttgca tctccggtcc
    13561 cagtctgatg agcggttact ttggcgacca ggtttcgcaa gacgagattg ccgcgacggg
    13621 ctggttagac accggcgacc tcggttatct gctggacggt tatctgtatg tcaccggacg
    13681 cattaaagat ctgattatta ttcgtggccg taatatctgg ccgcaggata ttgaatatat
    13741 agcggaacag gaaccggaaa ttcattctgg cgatgcgatt gcttttgtta ccgcccagga
    13801 aaaaatcatt ttgcagatcc agtgtcggat cagcgacgaa gaacgtcgcg ggcagcttat
    13861 ccacgcgctg gcggcacgga tccaaagcga atttggcgtg accgcggcta tcgatctgtt
    13921 gccgccccac agtattcccc gaacgtcctc cggcaagcct gcccgtgcgg aagcgaaaaa
    13981 acgttatcag aaggcttatg ctgccagtct taatgtgcag gaatccctgg cataaatcaa
    14041 actgtcgcgg tgacgggcgc taccgggttt atcggtaaat atattatcga taacctgctc
    14101 gcccgcggct ttcacgttcg cgcattgacg cgtactgccc gcgctcacgt caatgataat
    14161 ctcacctggg tgcgcggttc gctggaagat actcattcac ttagcgagct ggttgccgga
    14221 gccagcgtag tggtccattg cgccgggcaa gtgcgcgggc acaaagaaga gattttcacc
    14281 cgctgtaacg ttgacggcag cctgcgcctg atgcaagcag caaaagagag cggcttttgc
    14341 caacgttttc tgtttatctc ttcgctggcg gcgcgccatc ccgagctctc ctggtatgca
    14401 aaatccaaat acgtcgccga gcaacgactg gcagcaatgg cggacgaaat tacgctgggc
    14461 gtttttcgcc cgacagccgt gtatggtccc ggcgataaag agttaaaacc gctgtttgac
    14521 tggatgctac gcggcctgct gccacgactt ggtgcgccag atacacaact ctcttttctt
    14581 cacgtcaccg attttgcgca agcagtgggt cagtggttaa gcgccgaaac tgtacagacg
    14641 caaacctatg aattatgcga tggcgtcgcc ggtggctatg actggcaacg cgtacgacag
    14701 cttgccgcca aagcccgttg tggttccgtg cgaatggttg acattcctct gccggtactc
    14761 acaggccttg cggatatcag taccgcgttg agtcgcctgg caggtaaaga acctatgctg
    14821 acccgctcga aaattcgtga attaacccac gccgactggt cggcaagtaa taaccgtatt
    14881 tctgaagata tcaattggtt tcccgggatt agcctggaac acgcattacg caacgggcta
    14941 ttttgattgt cttgttttaa aggtgtgtta acgtttctat ggtaaatcgt gaaatagtaa
    15001 tggattacat cctttcctgt ttacaggatt tggtcgaaaa cggtgtggaa attaagcccg
    15061 acagcgatct cgtcaacgat cttggcctgg agtcgatcaa agtgatggat ctcttaatga
    15121 tgctggaaga cagattcgat atttccattc ctattaatat tctgctggat gtcaaaaccc
    15181 ctgcgcaatt aatggaaact ctacttccat ggctggagaa taaataatgg ggctatacga
    15241 taaatatgcg cgccttgctg gcgagcgtct gcaatttagt gacaacgggt taacaccgtt
    15301 tggcacctgt attgatgaag tgtattccgc caccgaaggc cggatcggca ataaaaaagt
    15361 gattctggcc gggaccaata attatctcgg tttaaccttt aatcatgatg ccatcagcga
    15421 aggtcaggcc gcgttagcgg cccaaggcac cggcaccacc ggttctcgta tggcaaacgg
    15481 cagttatgcg ccgcacctgg cgttagagaa agagattgcc gagtttttca atcgccctac
    15541 cgctatcgtt tttcaaccgg ttataccgct aatttaggcg tcatcagcgc acttgccgac
    15601 cataacgccg tggtgttact ggatgccgac agccacgcca gtatttatga cgtctgttct
    15661 ctcggtggcg cggaaattat tcgtttccgc cataacgatg ctaaagatct ggaacggcgc
    15721 atggttcgcc tcggtgaacg ggcgaaagag gcgattatca tcgttgaggg catctacagc
    15781 atgctcggtg acgttgcgcc gctggcggaa attgttgata tcaaacgtcg tctcggtggt
    15841 tatttgattg tcgatgaagc gcattcgttt ggcgtgttag gcgcaacggg gcgcggactg
    15901 gctgaagctg tcggcgttga agatgatgtg gatattatcg ttggtacttt cagtaaaagt
    15961 ctggcctcga ttggcggttt tgctgtcgtt tcggaagcga tggaagtgct gcgctacggc
    16021 agccgcccgt atatttttac cgcctctcct tctccgtcct gcattgcgac cgtgcgctcg
    16081 tcgttaagaa ctattgccac acaaccagag ctgcggcaaa agctgatgga taacgccaac
    16141 catctgtatg acggtctgca aaaactgggt tatgagttaa gttcacatat tagcccggta
    16201 gtgccagtaa taatcggttc gaaagaggaa ggtttacgca tctggcgcga gcttatctcg
    16261 ccgggcgtgt atgtgaatct tatcctgccg cctgccgcgc ctgcaggcat cacgctgctg
    16321 cgctgtagcg taaatgctgc ccacagtcgt gagcaaatcg atgccatcat tcaggcattt
    16381 gctactctga agcagtaatg tgagcccgcc gtggtgtttg cgccacggcg ggtttaatgt
    16441 tgtctgtcat accaatacac caggctcaac gccaccaaca caaacgctac cggtaaaccg
    16501 agcgcggtaa caggtgcgct taacgcaaat agcaatccca gattaaccat gatttgatag
    16561 ctgatccagg tgagtaagcc cacgatgacg cctacagcga gacggctacc catcccaggc
    16621 gaacgcgggg cactaaaggt aaacggcaca gccagcaaaa tcatcgccag ggtcaggatg
    16681 ggttgcccca gtttttccca cagtgcaagg tggtattcgc tgctgggttg cccggtttcc
    16741 tgcaaataat ggatgtaatg attaagctga cggacagaaa aagtattccc cggcattgat
    16801 aactcttcaa gattcatact ggtgaaggcc gattgccagg caagattatc taatgtttcc
    16861 agcgtttctt tgccattcag ccatttttta tgattcacac catgcaggac ccacgttttg
    16921 tcgtcttcaa tggtggcact gcgtgcataa atgtaggatt caagcgagag atcgtcgcga
    16981 taatgaaata tctccacgcc caccggctgg ccctgctcgt tcagcgattt caccgtcaca
    17041 aattcattac cacgcctggc ccacagcata ttgccggtaa tgtcatcgtc ctcccccaac
    17101 gcggtggcgg tcgatttgat ttgcaacgcc tgttgctgca atggcgacgc cacccactca
    17161 tctatcgcgc ctaacgaaac agtccacaat atcccggcaa ccagcgccac cagtgcaata
    17221 cggaagatag aaaaccccgt gctgcgaatg gcggtaagct cactgttttt cgataactgc
    17281 cccagtccaa caataccacc caataacgcg ataaacggcg aaagttcgac cagtgtgcgc
    17341 ggtaaggtca ttagcaccac cagcaccgcc tgagtccagc gataaccgcc cgggctgaca
    17401 ccatccagtt cgttaatcag gttgaaggtg gtaaaaagcg gcagcaatag ccctgcagcg
    17461 gcggcaaaac cgagaaagag atgacggatt aaatagcgac tgaaaacatt catcgggaaa
    17521 atttctgcag caaagagaag tcacgtgcga ccagtatcag caggcccatt agcatgagtc
    17581 cgggcactaa ccacaaacca ggaatgaggg gaatcgcgcc gttagccacc agtgtacggc
    17641 agacgtcgcc gccataaaaa atggcaataa acagcaacgt cagtggcaat aacgttgaaa
    17701 atcgcccttg ccgcggtttt acccgactta atgaaatggc cagcagcgcc atcaacaatg
    17761 cggtcaggcc acggctttgt cgccattgca gttcggcatg atcggcagga aaggcggagc
    17821 gcgccagctc cgtgaccgat ttcgctttgc gtttgacgtt cgggctttga tccagcggat
    17881 tcagatgtaa ttgcagatta cggtagatct gttcgttgtc gtcacgcccc tgatgatcga
    17941 gaagataggc agtgccgttg tgcaacataa cggtcggttt ctcgggtgat gggtcaacca
    18001 catcaaccga accggcgcgg aaaatacggg ttcgattggc agtagaagta taaatcagcg
    18061 cgtcagtcag gcgattatta tcctgatcaa ccgtctgcga aaggatcatt cgtccgttat
    18121 cgttagtgtt aaatttcttt gcccgcaact ggcgaacatc cagctccgac tgcgactgct
    18181 gctccagttg ataaatctgc gcataagccc acggacgacc atacatcgac agaagggtga
    18241 caaaaatact tagcggtatc gccagataaa gaacggcttt gtacaagcgt cccggactgc
    18301 cccccgccgc agagatagcg gtaatttccg agtcggtata catctgccct agcgtcacgc
    18361 caactgacac atacagacca acaggtaaca acatctccag tgcaatcagt actttgtaga
    18421 aaacgatatc cagcacaaca tcaagagcta atgtcccatt tgccgcttcg gtcagataac
    18481 gctgtgcgga gtagctggcg aaaataaaga tcagaaaacc gacgataatc agcaccagac
    18541 ggcgcgtacc acgcatgatg tagtgttcaa ccagcttcat ctcactaacg ccctcaatcc
    18601 tttgagtttt ttattcattg atcagtttct gcgccaacgc aatgacatga tctacctctt
    18661 ccggcgtcag gctgccgtcc tttgcccact gaatttgccc ggctttattc agcaccagaa
    18721 tggttgaact ctgctccggc aagcgccagg caacgcgtcc ctgtccattg ccgtcaataa
    18781 cgaattgcgc ccacggatag cggcgtttat ttttttcgat tttgccaaca acaaaatagc
    18841 cagtaccaaa gatggcatca tcggtgttca caatggtggt gggctgaaaa cgatcctgcg
    18901 gaaagtttgc cgcttcaacc gctttaatta acagtgagtt ctttttcttc gccgacttac
    18961 gcccggcgat atactgaata atgcggactt ttcccgctaa ttgtgtgctg ttccactttt
    19021 ggtagtgaat ttcgtcgtcg ctattcagaa gcagctcgcc gccttcctgg atataaacag
    19081 gggtaaccgt tttgccagtg acgaaattgt gtgcctgcac acccggggct aagagcatga
    19141 taatcaacgc aataatcaaa cgagatgaca tcattgctcc ggctatagcg gaaaacgcga
    19201 aatcagcagc tctaagcgct gacaaatatc gctatccagc gccaacggtg cataatacga
    19261 ctggcggttt tccatatcat ccgccaccat gtcgtcgtgt ttatctccca tcaggccatt
    19321 aatggcggcg aggaaacggg aaatctgtaa cgcctcttta ttttcaggat caagcgatct
    19381 taaggtgtcg taataagaaa cctgctggct gatgattttt cgcacatcat gaaattccct
    19441 gccggttagt cgcggtttgg caataatctc tttcagggta tgcatttgct tttgccgata
    19501 ggcaataaaa ctgcttacgc tttctaactg gctatggcgt aatgaatagt tcaccaacga
    19561 ccagaccgga gagcttaaat aaatccgtaa caggttgccg taatacgaca caatattttt
    19621 cttaccgtta cgaaagccgt cctgaaaacg ccccagaaaa acggtcgttt tgccgaataa
    19681 aaatccggcc tgatgcttct ttaaatacag ccgttgcgca aaacgcaggt gcttgtagcg
    19741 cgctcgcatg tatttaaatt gtttgcgctc atcagcagtc agctcatcgc ctttggcttg
    19801 tttgcgaact aaacgcagta attcctgacg atcaacgcca ttgagccaga attgcaggca
    19861 taacgcgtaa ttatcatgaa tgatttcggt ggtacaactt agtgcaatag tcggcggcaa
    19921 actaacctgt gcatcaacga catcatcttc ttcaatcgca acaaatagac gctcgctcgc
    19981 ctcaagcggg aaatattttc tcatcatcag gatttctctc cggctgcgtt ataaagcaag
    20041 gaatggcgct gaaaaaatgc ctttaaggtt ggaattaacg agagcgccac gtccagacat
    20101 tgcccaccga agtaagcaaa cgctgctccc agcacaccaa acagatgaca gaacatgaac
    20161 agcaatacga tgtagccgat tgaggcgatg gtttgcgcca cgagaaaagc gcgctgtttt
    20221 cccgccatca atagcaatga ctcctgcggg aagcccagca tcgaaatcac aattgcgccc
    20281 agcatcacct gaatcaggtc atacgcttcg agatacttaa cgccaaacac cagcgaaatg
    20341 agcggcttgc cgacgatcaa caccgcgagc gccaccagaa taccgattcc gcccgccagt
    20401 aaaccggact tcacacctaa caaccacggt ctggtggtgc gcggatctaa acgcatcacc
    20461 tctgggtaaa aacttttacc cagcaaaccc gctggcgttc cggcggcgtc gaagaatgtc
    20521 atggcgattt taaataaccc ggcggcagcg ggtcctaaca cgatccccac caacactgtg
    20581 ctgcacgagt tacgcgccga ccagatggag tgggcaatgt tggttgacca gacaaaactc
    20641 cacgcgcctt taatgtgtcg ggcagactca aacagattca atttgaaggc gttatggata
    20701 tttctacggc gtaattcgcg cgcggcaaac caccagtaca tggtgccgcc aaccagattc
    20761 gacacgtacc aggcaataac aaaacccaca aaaccaaagt caaaatacca ggctgcgacg
    20821 ctccccgctg cgcgcagaaa aggtttcgtc gcctgctgta cagcaatcaa atcaaagcga
    20881 tctaccgcac gcagaatgcc ggtcggcgtg gaggaagcca ttgaaggaat gagcgtgcaa
    20941 tacagcgccg ccagccaaaa gctttggtca tccagaccta atgaatggga aaggaatggc
    21001 agtaaggcaa tgccgccgac aatcgccacc gcgccgctga cgatatccag cgagaaggaa
    21061 aatgagacga cattgcgaaa ttgctgcgga ttattgttgg tcaaggctgg cgttccgtac
    21121 tgaaccacca gctgccatgt ctgaaactta ataaaatcgc tgatcgactt ggcgtacgat
    21181 tgcacaatca ccagtacacc aaacatggcg ggcgtcatcc ctttaccggc acacgagagc
    21241 gccaacagcc ccagcaaggc gctctcgaca ttactggagc ctaaccaggc gctattgcga
    21301 ataatggtgc gaaacgcgcc atctgcaaac caatgtttga tgttaaaacc cgccaattca
    21361 gcctgacctt atcgttgata gtaaaaaata tcccgccagc cttaagttaa acttcggcgg
    21421 tcagaaacga tggcaaccag agaaaccgcc ttctgtacct gttccagcac ttcgctgtag
    21481 ggcgctctgg aatcaatctc aagaattttt gtgccgttat agccaatcgt cgacatgacg
    21541 ccgattttgt cctgcagctc ggcatactca tggtcaggct tgcgggagat ggcagtatca
    21601 atatcaatcc ccaaacgaat gattaattca ggacggtatt gcgccatttt ttggtataaa
    21661 ctccgttcgc gctgcgccag aaacatgctg attttcccgg tcgcacgttc gacgccgatc
    21721 cccggtccgt cataataaaa gcccgaaatt tcagcctgcg ggaagcgatc gctgaccacc
    21781 agaacgccac tttgcgccag tcgctgaacc ttgcgtagat tcgccattct tcgcagcgag
    21841 aagcagtaca taatcaccgc cgcccatagc gccggagatt tggttttcat gctttgggtt
    21901 ttcgaggatt tggccgccaa tcgccgttcc agccagacgc caaccaacgg caatcgtttg
    21961 attttgtcgc cgtcttcgcc ggagagcagc cccagatagc ggcgctcggt ttgccagtgt
    22021 tgttgcagcg atttcaccag gtcggtggtc agcgtggatt taccggtgcc atcacacccg
    22081 accaccgcaa tcagccccgg aatgtagttg ggctgcggcg cggttgtact attaacagtt
    22141 tgtgtttgta gtgcatccat tcgttaaatc atcccctatc cattttggtg atcaggaaag
    22201 cgcggcgtga attgcgcgta gtgacgcttg cataatttca tcggctggtt gccgaccatc
    22261 cagttcaagg atcttggcgc cattgaatgt cagttgcggc gtaacggcga ttttttcctg
    22321 taacgctgcc agttggtggt caggtttacg cgcaaacgcg gtttgttcat caatgccaag
    22381 acgaatcaac aatacgggta aataagacgc catccattgg tacagcttca actcgcgctg
    22441 ccttaacatt tttatccaac cgttaccgcc cgtggttttt gccaattgcg ggccatcaaa
    22501 gcgaaaaccc ggcacttcaa cctgcgggta gcggtcggtg atgagcagat agccttgctg
    22561 gcttttacac aacattttgc gaaacttgta cgcccgccag caggaaagca gatagataac
    22621 cagcgcagta atattgccag gcggagttaa gggcttttcg tgtacatgtg ccgctttact
    22681 tcgcagataa cacccacaag gtgcgccaat aacagggagc tgtgaaatcc attcgccaat
    22741 tcgcccggac gattgcccga gataaatgtg ttctgttggc attcttgctg ccagttcatt
    22801 taccaggctt gccgtgaggg tcgatttacc tgaaccatca catcctacaa tggcaataac
    22861 acgcactggc gtggaattaa ttattgacat attacgttga ttcacgaaaa acccggcagt
    22921 aaataatgta ttgaatatta cgtggtccgt gctcagaata tccgttcaac cttattgtga
    22981 caatgactaa ttgtgacatt gacctgaatt acgctttcat aaaaacatat taaccaaata
    23041 aatattttta atggatattt aaattaaagg atatattcat gcagtcaata acacctccat
    23101 taattgccgt tattggtagc gatgcttcag gcaagtcaac ggtgtgtgaa catcttatta
    23161 ccgttgtcga aaaatatggt gctgccgaaa gagttcattt aggaaaacag gccggaaatg
    23221 tcggtcgtgc agtgacaaaa ttacctttga tgggaaaatc cttacataaa acaattgaac
    23281 gaaatcaggt gaaaacagca aaaaaattgc ctggaccagt tccggcgctg gtaattacag
    23341 cgtttgtcgc ccgtcgctta ctgcgctttc gtcatatgct tgcctgtcgt cgtcgcgggt
    23401 taattgttct aaccgaccgt tatcctcagg accaaattcc tggcgcttac gatggtacgg
    23461 tgttcccacc taacgttgaa ggtggtcgtt ttgtctcatg gctggcaagc caggaacgta
    23521 aagcgtttca ctggatggcg agccataagc ctgatctggt catcaaactc aatgttgacc
    23581 ttgaagttgc ctgtgcacgt aaacccgacc ataaacggga atcgctggcg aggaagattg
    23641 ccataacgcc acagttaacc tttggtggtg cacaactggt tgatatcgat gccaatcagc
    23701 cactggaaca ggtgttggtt gatgcagaaa aagcgattac ggattttatg accgcgcgtg
    23761 gttatcacta gtcaaaaatg gaaatgcccg atcgccagga ccgggcattt tcaggaaggt
    23821 taaatcaact gcaatgctat ccagtacagc ccaccagaaa gaaaaattgc cgccggtaaa
    23881 gtaaacaccc acgccatcag gatgctggtt accgttttac gctgtaaccc accgccgtcc
    23941 accaccatcg tccctgctac cgcagacgag aggacgtgtg ttgtggagac aggcatccca
    24001 atataactgg caaggccgat agacactgcc gccgtcattt gtgccgccat gccttgcgca
    24061 tacgtcatgc cgcgcttacc aatcttctca ccgatggtca tcgctacacg acgccagcca
    24121 atcatggtgc caatgccgag cgccagtgct accgccatga tgatccacac cggagcgtac
    24181 tcaatggtgc ttaacatatc gctgcgcagt tttttcagca ggttctggtc ttctttactg
    24241 acgcctggca gtttcgccag cttcgcggag gtatcggaga tgcacagcat aatgcggcgc
    24301 agctggctgc gctgactcac gcttaacggc tcgtaacttt ccatattgcc tggcagcatc
    24361 gttttaacgc gcgcaatcgc atcaaaggta tttgccggat gacagtgaaa ctctgttact
    24421 tgcgtgccat cagtcgatgc tgcaggcaat ggaggttcca tcgcaatcaa cttctgcggc
    24481 agttcaggat gctgttgcag gtagtgttcg aagttggtaa cggcatcgcg ggtacgggta
    24541 atttcatagc cggacgcatt catattgacg acgaagccag caggggcaat ccccaccagt
    24601 accagcatta ccaggccgat ccctttctgc ccgtcgttcg cgccgtgcga aaacgccacg
    24661 cccgcagcgg aaacaatcag cgcaatacgc gtccagaatg gcggtttacg tttgcccttt
    24721 ttctttttgc gatcttccgg aatgcggtga atacggtcac gctttttcgt cccactccag
    24781 aaacggcgca gcaggaaaat cagaccaccc gcaatgacca ggccgacgat aggggaaaca
    24841 atcagcgagg agaaaatttt ggttacttca cgcaggttta acgcatccat caccgatgag
    24901 ccggttaaca gcgcgttggt taaaccgatg ccgataatcg caccaatcaa ggtgtgcgaa
    24961 ctggaggccg gtaaaccaaa gaaccacgtt cccaggttcc agataatcgc cgccagcagc
    25021 atggaaaaga ccatcgccaa accgtgagtt gaccccatat tcagcaacaa atcgattggt
    25081 aacatatgga caatggcata ggcaacgcta agtccgccca ataacacgcc aaaaaagtta
    25141 aaaaatgccg ccatcaccac agcaagttgt ggctgcatgg cacgagtata aataacggtt
    25201 gccaccgcat tcgccgtgtc atgaaaacca ttgattgctt cgtagaacaa cacaaatgcc
    25261 agagcaagta ataacaaaag ccccgtatat atatcaaggc caacaaataa atttagcata
    25321 tattagatta cgccattttg aatttacgaa cggacgcatt atcagtgact ttaacggcat
    25381 gggcaaagtg aaatatcatt ttttaacgat aaaaagagtg ttatttcttg tgtatcagac
    25441 aataagcatt cattcaaaac ctttagaaat aatcaaaaca caggtattcc actggttaat
    25501 atattcacat atgaaatgaa taaatattgg aatatataaa tattgaatat tttgattaat
    25561 ccccgtacta attattcttc ataatcagta cggggttgct acaacatgta ttactttttc
    25621 accacaatta acggttcaat atcactctct tttttgatga ccagtgattc atcaccgcgc
    25681 aaacacgtcc caccgtagtt gccgccaacg gtgaaggtac atacctgaat gtatttaccg
    25741 tccactttcg gcaaacacca cagttgctga tagatgtttt tctgctcggc aaatttaccg
    25801 ctggttttgt ccagcacctc ttcatgatgg ctgaccaggt cgatattgct accacagcga
    25861 ccggcgatcg gtttcactgc gtaacctgtt ttcaccaatt catcattaac agtgaaatcg
    25921 gtatccagca ggtaacgatg gtgcgggaac agcgaccaga ggatcggcag aatcgctttg
    25981 ttgccgggga tcaccgtcca cagcggctca aagaccagca cttccgggcg cagcaatacg
    26041 tcgataagac gcacttcgtt ttgcggatga ccggtacgga ttggcaccgc agcaaactca
    26101 cggtcgctaa cttcacgaat ctgatcaaac gcggtttccc acgcccaggt tttccacacg
    26161 cagttaacca gtcgcccttc cccatcaatc agttgcccgg cagcatccca gcccagttca
    26221 tccagcccgc gcaagatacg cgtttcaaag cccgcctggt gcagcgcctg ctccataaac
    26281 tgcgcgtgat agttttcctc gatatctttg tcctgcatga tatggacaaa cggacgtgca
    26341 cgactgtgtt tccaggcacc agccagttcg ttaatcagcc cttccgccgg attgaagccg
    26401 ttgcctttat agccctgctc cgcccaacgt tcgaggatca agcccgcttc ggtatgacag
    26461 gaggcggagt cggcgttgta ttcgtaaacc ttcaggccac gctcatccat gcagaaatcc
    26521 atacgaccag tgatcatatg gtgacggcga cgctgccagg agagacgcaa acgtggccag
    26581 aggattttcg gaatgtcgaa cagcgccagc aggttgtcat ctttcagcac cttgtcggtt
    26641 gcgtgaagat acatcagatg cagctcgttg gtggctttaa ttagctcctg ctcggcactc
    26701 tcggtaatgg tgtagtaatg atacggatcc tgattgatca cctgaccgtt ggcctgcaca
    26761 taggcgtttt gtagcggatc tttttcatcc agccatttac cgtcaaactg gcctttgttt
    26821 tccagacgcg ctccactgat tttcagcagc tcgcctgcaa tttccggctg cggtaagctg
    26881 tattcagtat cttccgtctg gatcatccag cccagaatgg tggtgtcatc aaaagtgtct
    26941 ttaagggtat agcagccgtt ttcgaccacc atctccagct cgcgcgtcca ctgttgccct
    27001 tgcggcaacg gggaatgaat cacgttctgt tccgcaatac ggactttgtt gccatgcaat
    27061 tgggtaatga tggcgacatg gccagtgtct ttaaattcac cgcctttatc ccagataaga
    27121 agcgcacccg cgaccggcgc acgcggcgag ccgttaggaa atgcctgcaa tggcaggatg
    27181 ttgtcattaa ccacttctcg caggaagcgc agcgagaaaa tctcccacgc catacccacg
    27241 tcagtaaaga ccacaccgta attcagaaag agaaaacggc gagcaaattc aacgcattgc
    27301 cacttgtggc ccatatattc gtcgtcgata tagctacgga atacggcgtc atcttcgtat
    27361 tcctgcggat cgagagaact gtaatctgaa gagtagattg ctaccccacc tggggcgtag
    27421 cccaataatg tcccgaacgg ggcatcctgg ctggtcgttc ctttgctcat cactttacct
    27481 ttaacaatac aacctaagca gttggcgtaa ttgttgtgct ctgattacct gctcagcgac
    27541 attaaccgga cagaggtcag actaatcata cacctcatcg cagcggctgg cgcaaagggt
    27601 taaaaaattc acattctgta cagcaagtga cctgctacac tgcttcaaca ctaccactca
    27661 gaaggcaact cactatgaca gacaatactt atcagcccgc gaaagtctgg acgtgggata
    27721 aatccgctgg cggcgcgttc gccaatatca atcgcccgtt ttctggtccg acgcatgaaa
    27781 aaacgctgcc cgttggcaaa catccattgc aactttattc gctgggaacg ccgaacggtc
    27841 agaaagtaac gattatgctt gaggagctac tggcgctggg cgttactggt gcagagtacg
    27901 acgcctggct gattcgtatt ggcgatggcg atcaattctc cagcggcttt gtcgaagtga
    27961 acccaaactc gaagatcccg gcgctgcgcg atcatacgca taatccgccg atccgcgtgt
    28021 ttgaatctgg ttcgatcctg ctttatctgg cggagaaatt tggctacttc ctgccgcagg
    28081 atctggcaaa acggactgaa acgttgaact ggctgttctg gttacagggt gccgcgccgt
    28141 tcctcggcgg tggttttggt cacttttacc attacgcgcc ggtgaaaatt gagtacgcca
    28201 tcaaccgctt taccatggaa gccaagcgcc tgctcgacgt gctggataag caactggcgc
    28261 agcataagtt tgttgcgggc gatgagtaca ccattgcgga tatggcgatt tggccgtggt
    28321 ttggcaacgt ggtgctcggc ggcgtgtatg atgccgcaga gtttctggat gcgggaagtt
    28381 ataaacatgt gcaacgctgg gcgaaagaag tgggcgaacg tccggcagtg aaacgtgggc
    28441 gtattgttaa ccgcaccaac gggccgctga acgagcagtt gcatgagcgc catgacgcca
    28501 gtgattttga gacaaataca gaagataagc gtcaggggta agggttggtg ttcgtcgcag
    28561 caagccatcc aggccggata aggcgttcac gccgcatccg gcaatcgtgc acaatgcctg
    28621 atgcgacgct gccgcgtctt atcaggccta caaaagctaa tcatcacgcg ctggtaatgt
    28681 cgtaatccat ttggcgcagt gcgtctaatg tggctttggc ttcatcttcg tcgatgatgc
    28741 tcatggcaaa tccgacgtgt accagcaccc actggcccag cagatcggca gggttacctt
    28801 cgcaaatcag ggcgatattc acgtcgcgct tgataccaca tacttcaacc tgcgcaagct
    28861 ggtgaatatc ttcaccgaca gccagcacct ggcctggaac gccaatacac attgttaact
    28921 ccggttattt cgattgccgg acaaacgcga aaaagaacct ttagcgggtg ttccctctca
    28981 ccctaaccct ctccccggag gggcgagggg accgtccgtg cgctttttag gcgttgtcct
    29041 cagtcttatt caacttcaat acttttgacg atcagcgaat cgccggtatc gacacgcaac
    29101 cgctcgccgt gacagagcgg acactgcgca tcgtgctgat gaatctccac cacctggctg
    29161 caatcccagc accaggcctg ggcgggttta tagacgatat gtaaatcgca cccttgcgcc
    29221 accgttccgt ggcagacaat ttcaaaacta aaacggacgg cgctctcctc aacgcaggag
    29281 agcgcgccaa tttccagcca cacggcggtg acgcgcttaa catcgtgctg ctccgcctgc
    29341 cgttggataa tttcaacggc gctctggcaa agcgacaact catgcatttt cgcccctcct
    29401 gcggccaaac agcagggcgc gacgccctgc gtgtggtaca tccggattgg tgactggcaa
    29461 cgaaaggatc attcgtgcgc agtcatccgt caggcgttgg ccttcttcaa tcgacatgct
    29521 gtgcgaaagc ggcgacatca gcgaacagga gaggtattgc gaaacaccgt ccagttcgcc
    29581 aacggtaaag gtcatggtgc catacggcag ttgcagacca attttttcac tcactttgcg
    29641 cagcggccag agttgatccg ggccggggaa gataactgca ctcagcatcc acggggtgat
    29701 cacacacccc gtccactgac cttcgaacag cgtaaaatca gaaacataca ccggcattga
    29761 aggatgcaga aaagagagat cgtgcatcga acgccgggca atttcttcaa acgctgcctg
    29821 tacttgcgcc ttcggggagg tctggaaacc tgctatctct tcagtcatga atcgcctccc
    29881 gtgggatggc ttccacgcct gattcgcgca gcgcagccag aacctgctca agcgcaggtt
    29941 caatcattgc ttcaaccgtc ggcgttaagc cgatgtgtgg ctccagcgat tccgggatca
    30001 cgccgaccag ggtcagcttt ttcggaaact caccggtgaa gcgcagggcc gacaatacgt
    30061 cggccaggcc aagctgatgc ggagagattt tgttggtaaa caacgccgga acttcttcat
    30121 cccgcaggat catcatcgtt cccggagtgt tctttttcga cacaatggca tccgcaataa
    30181 tcagatgatc gcgatttgcc atgtcgccaa gcagctccat tcccgccgtg ccgccatcga
    30241 ggatctcaac ataatccggc agaatgtatc gttgctctaa cgcttcgaca atccgcacgc
    30301 cgatggcttc atcggtcagc aaaatattgc cgacccctaa gactaaaata cgcattacag
    30361 aaccttcact gaaaccactt cgttgccgtc ggcatccact acgtgtaccg cacaggccat
    30421 gcacgggtcg aaggagtgaa tggtacgcac cacttccagc ggtttattcg gatcggcaac
    30481 cggtgtaccc accagcgact gctcgtaagg accgacgtca tcattgaagt tacgcggacc
    30541 agagttccag gttgatggaa caaccgcctg gtagttgctg atgataccgt ctttaataac
    30601 catccagtga gagagcatac cgcgcggcgc ttcgaggaag ccaacacctt tgaactcacc
    30661 cgttgccgga atgttcggct tcacaaaggt ggtgtgatcg cctttgccga tattggtgat
    30721 cagtgcactg tattggtttt gcaggatatc ctgcaattca cagcagtgaa cggtacgacc
    30781 aataatacgg cccagcgtgg agtgcagctg tgccacttcc agcgtgttgc cagtcagttt
    30841 ctgataaatc gcaacgattt cattcagttt gttctgggta gattcgcgac ctgccgccag
    30901 tttcaccagc atattagcca gcggacccac ttctaccgtt ttgccgtaga aagtcggtga
    30961 tttcacccag gaatatttcc cgtcgtcaga ccaaccatca taagccggaa tggtggtacc
    31021 ttcccacggc gcctgtggcg cttcgtcttt ataccaggag tgcttcgcgc tttcctgaat
    31081 gcctttgatc aggtattcat cggaatgaga agtgatcgga cgatacgagg acagatccgc
    31141 attctcaatg tagcctcccg ggaacaggaa gctgccgttt ttgctgtcgg tcgggaattc
    31201 cggcacgctc aggtagttca ccgcaccttt accgcgcgtc agccattccg ggtagaacgc
    31261 ggcgataacc gcggtatcaa ccttataaac ctgctcaaca aagtcgctca gtttgtcgat
    31321 gaaagacttg atgtacatca ggcgctcaag gttcaacacg cccaacccgt cgaggttgat
    31381 tgggttcgcg acaccaccca cagccaggtt ctgaatgtgc ggcgttttac cgcccagcag
    31441 cgccacgacg cggttagcgt cacgctggca ctccagcgcc tgcaggtagt gcgctaccgc
    31501 aatcaggttc acttccggcg gcagtttcat cgccgggtga ccccagtagc cattggcgaa
    31561 aatacccaac tgaccgctgg caaccagatc tttgatcttg ttctgaactt tagtgaactc
    31621 ttccggactg ttcagatgcc aggtcgaaac gcctttcagc atttcggagg ctttggttgg
    31681 gtcagcttgc agtgcagaag tgatgtccac ccagtccagc gccgaaagct gatagaaatg
    31741 aacgatatgg tcatgcgtgg tgtgcgcagc cagaatgatg ttacggatgt attgcgcgtt
    31801 aaccggaacg tcgatattca gcgcactttc tgccgcacga acggaagaca gcgcgtgagt
    31861 ggtagtacat acgccacaga tacgttgcac aatcatccat gcatcgcgcg gatcgcggtt
    31921 tttcacgatc tcttccatgc cgcgccacat ggtaccggaa gcccatgctt tcgaaacgac
    31981 gccattttcg atttcgcaat cgatgcgtaa atgcccctca atacgggtta ccggatcaat
    32041 agtaattctc tggctcatgc tttgctcgcc tcatgacgat tatgatcgtt ttgttttaaa
    32101 ggaggaagta tcggcagtag acgaatgagt acgatgtaag cgcaaatctc aatagccaca
    32161 aaaccaatag aaatcaacag ttcttcccag gtcgggaagt aggcgtaacc gccgcccggg
    32221 ttgaatgcca ccaacgaata ggtcagacgc caggttgcac aacctaacag tgcgctcagt
    32281 gctgacagga acagcatacg ggaatcatta cgcagcttcg ccacacgcag aacgaccagc
    32341 gggaagagca tcagcaggac ttcaatccag aacatcacgg agtagaagtc accggcaaac
    32401 gctaacgaca gcttgtcgcg atagataagc tcgccaaagc gcagcccgat gaaaatcgcc
    32461 agcaacacac tgatggtgtt ggtcagcttg acgaacaggc tcttttcatc cggaccgttg
    32521 ccacgcagac ccgcctgcac cagtgaacct tcaaagatga caatcgagaa gcccatgatg
    32581 aacgccgtca gcagcgagaa cagcggcaac atttcatagc tctgccagag cggatgcacc
    32641 ttgtagcccg ccgagatcat cagcgacccc attgaagact ggtgcatggt cggcagcagc
    32701 gcaccgagcg cgatgatgaa gaacatcacc ttgttcagac gttgtagcga caccttccag
    32761 cccagacgtt caaacagtgc cggagcaaac tccagagcca tcacgccgat gtagatggtc
    32821 atacagaccg ccgtctcgaa cagtaccgag ttcacgttga agtgacccgg aatgtagaag
    32881 tacggcaggt tccagtagcg acccacgtcg atagtgatcg acaagccacc cagtgagtaa
    32941 ccaaacagac tcgccaacag cgccggacgc accagcggat ggtattgccc acggttaaag
    33001 acgtataccg cccacgccag cgcccagccg ccacaggcaa agccggtgcc aatcagcagg
    33061 tcaaacgcga tccacacgcc ccacgggaag ccgccgttca ggtcagagac agagcccaga
    33121 ccgaacacca gacgcttcac aatcaggagc atacagatga cgattaacgg tccaaaaatc
    33181 atgaccggtt tactgatgat tttgccgccc agcggttgtg gatcatgact catgatcgtc
    33241 tcctccgtcg tgatggtcgt ttttggtgtt gcgacgaacc agcacggtta agcccgccag
    33301 cacagccagt ggtagcatca tgcctttata cagggtgtgt tgaatatttt cggaacgcgc
    33361 accggtagaa agatcgtcca gtttcggcag gtcgagattt tcataaggca cacccgtcag
    33421 taccagaacc tgagtaccgc cgccctcttt ctcgccgtac agatgcggat aatatttcgg
    33481 caccgtatgc aggtaagtgt cgccagattt cagcgtctga cgcggatagt ggtattcgct
    33541 gccaggcttc agcgccagac gttttttcgc ctccgccatc agctcttcac gcgtaccgaa
    33601 aatcaccgcg cccgccgggc acacttctac gcagccaggc agaccgcctt tatcgagacg
    33661 ttccacacct ttctggttgc acagctcgca cttatgcagc gcaccaaacg ggttgttgta
    33721 gtcgtacttc ggcacgttgt acggacaggc gaccatgcag taacggcaac cggtgcacac
    33781 gtctttgtcg taatggacaa tgccggtttt cggatctttt ttcagtgcag agaccgggca
    33841 cacagagaca cagttcggat cgacgcagtg catacactgt ttcttaatgt acgcgtagcc
    33901 gttctcctcc tggtctttgt tgacccctgt gccgctggtc cacacctgaa tgatgttatt
    33961 ggtatacggc gacagtttgt cgttgttcga ccaggtctgt tccccttgcg ggttacgttc
    34021 agggaagttg atatcctgac acttggtgac gcaagcctgg cagcctacgc acaaggtcga
    34081 gtcgtacaac atccccagcg atcccggaat tggcgggcgg ttttcagcag ccgcatgact
    34141 gacagacggc agcgcgcccg tcagcaatgc cccgcaggag gctgctttaa taaaattacg
    34201 tctgttcacg gttattctcc ccgtgagtca gcgttatctt tcttttgctg acgacccagt
    34261 tcacgcaccg ccatcacgct gacaccggca accagcccaa ccacaccgcc gagcaaacca
    34321 atagcgcctg cagagacgtt gccgccctct ttagcgttaa catccggttt ctgtgaacgc
    34381 ggagtctgat tttcgacgtt ggcaagctga tggatgcctt tatggaagcc gatacctttt
    34441 tcgttacagc catagcacgg gtgaccaatc gccaccggcc acacaccgcc aacatcgcag
    34501 aattgcagcg ttgagcagtt gccgtaagtt tctggccctt tacagccgag gtggtacaag
    34561 caccagcctt cgcggtggcc ttcatcaccg aactctttgg caaaacgacc agcatcgaag
    34621 tgcgggcgac gttcgcagtg ttcgtgaatc agacggccat aggcgaaggt cggacggttt
    34681 ttgtcatcca gtttcggcgg tttgccgtaa gtgatgatgt gcgcaacggt cgcgaggaag
    34741 ttgtgcgggt tcggcgggca gcccggaata ttgataacgg ttttgcctgg cagaacttct
    34801 tgcaagctga ctgcgccagt tgggttaact ccagctgcgg caacaccgcc ccacgcggag
    34861 caggaaccaa tagcgataat ggctgctgag ccttccgccg ctttgcggat gtgatccaca
    34921 atcggctcac cggcaaccat gcaataaata ccgttatctt ttaatgggat ggaaccatcc
    34981 accactaaca catactgccc tttgtacttc tcgagagcgt tatgtttgtt ctcttcgacc
    35041 tgatgaccga aggcggcgga aagcacttcg tgatattcca gagagatagt ctccagcacg
    35101 aggttttcta ccgttggatg cgttgcacga agcagagatt ccgtacaacc ggtgcactcc
    35161 tgcgcgccaa tccagataac tggcggacgc tgcgggttag taaccgattc ggccatctct
    35221 gcagcggctt tgctacttaa ccccatggtg gcggctaatg ctgcacaaag cttcatgaaa
    35281 tcacgacggt taatgccgtg agaatggatg agggtgttat ctccagtcat ttatagttat
    35341 tccgttgcga gaacctggca tatattttgc ctcaatcgca aaatcaataa tgcgatcgat
    35401 gcgccattta ccacacattt attatggtta tcgatatcat gatactgcgg cggggcgcta
    35461 aaacgaaggg aagaagcatt agtgtaatta attaaagcaa gataatacgt atgtttgatc
    35521 aattttcgag gcgataaccg gccataaaaa agcgcaacgt agaacaggaa ttattctcac
    35581 gaaagaaatc cattaccatc tctttatcca gtccatagcg gcgcgtcaga atacccgctt
    35641 cccaggcgct aagctggcga tctccggtct tttcgtacat cctgtgtgag tagcctaata
    35701 caaaaccgcg tttataatcg gcacagaatt ttgtcacatt aaccgcactg tcggcatgcg
    35761 ttgcttttaa tcccgccatt aagcctttac caaaatggtt attcatcgtt cacctcattc
    35821 attatatatc gccatatata acgatatttt aggcaaagaa cgacttttgc gcacgctatt
    35881 ttttcatact tgtttaagac gtaatcccgt aagagataaa cctatcagga gagatattta
    35941 tcgaaccatt tcagtgtcct ttgccaggca agatcggcgg cagatttgtc ataacggggc
    36001 gtggaatcat tatggaatcc gtgattaacc cccggataga tataagcctc ataaacctta
    36061 ttattgtctt tcaacgccgc ctcgtaagga ggccagccct cgttgattcg ggtgtccagt
    36121 tccgcgaagt ggagtagtaa aggcgcttca atcttcgcca catcggcagt gggtgcctga
    36181 cgaccataaa acggcaccgc gcaggccagt tccggatacg cgacagccgc cgcgttcgat
    36241 acgccaccgc catagcaaaa tccggtaata cccactttac ctgtcgcttg cggatagcgt
    36301 tgcataaact caattgcggc aaagaaatca ttcatcagtt tggttggatc aacctgctgt
    36361 tgcagctcac gacctttatc atcatttccc ggataacctc caacggaact taatccgtca
    36421 ggtgccaggg cgatataccc cgccttcgcc actcgccgtg ccacatcttc gatatacgga
    36481 ttcagtccac gattctcatg caccaccccc acggctggcg ttttgccgct catttttgcg
    36541 ggtttcacca gataaccccg tacctcgccg tgaccatttg gcgaaggata cgtgatgtac
    36601 tcagcaacaa tctccgggtc ggtaaactct acctgagtcg ccagcgcata atttggcttg
    36661 agcaaatcaa acaacgctaa tgccgtcatc ccgcccaccg catacttcgc cgcaaggttg
    36721 aggaactcac gtttcgaaat tttcccgtga acgtaatagt cgtagtaatc caacaactct
    36781 tgtgggaaat ctttggcggt taaacgcggc atcgttgcat tcctcaactg gtgttttttt
    36841 aagcaaagca taagcgcgtg tttttgccca gtttttcgtc actctgtgag ccagactacg
    36901 ggatacgcgc tggcgaatcg ctaaactaga aacattgttt cgaaattgaa cggtggaaag
    36961 gagaggtcat ggtctggtta gcgaatcccg aacgttacgg gcagatgcag taccgctatt
    37021 gcggaaaaag tggtttacgc ctgcccgcat tatcgctcgg tttatggcac aatttcggtc
    37081 acgttaacgc gctggaatca cagcgtgcaa tcctgcgtaa agcgtttgat ttaggcatta
    37141 cgcactttga tttagccaac aattacgggc cgcctccagg aagcgcagaa gagaactttg
    37201 gtcgcctgct gcgcgaggat tttgctgctt atcgcgatga actgattatc tctaccaagg
    37261 ctggctacga tatgtggccc ggcccgtacg gctctggcgg ttcacgtaaa tacctgctcg
    37321 ccagcctcga ccaaagcctg aagcgtatgg ggcttgagta tgtcgatatc ttttactctc
    37381 atcgcgtcga tgaaaatacg ccgatggaag aaaccgcctc tgcgctggct catgcggtac
    37441 aaagcggtaa ggcgctgtat gtcgggatct cctcttactc gccagagcgg acgcaaaaaa
    37501 tggtcgagtt gctgcacgag tggaaaattc cgcttttaat tcatcaacct tcgtacaatt
    37561 tactgaaccg ctgggtggat aaaagcggcc tgctggatac cctgcaaaat aacggcgtgg
    37621 gctgtattgc ctttactcct ctggctcagg gattgctgac cggaaaatat ctcaacggca
    37681 ttccgcaaga ttcacggatg catcgtgaag ggaataaagt tcgtggtctg acgccgaaaa
    37741 tgcttaccga agccaacctc aacagcctgc gcttattgaa tgaaatggca cagcagcgtg
    37801 gacaatcaat ggcgcaaatg gcgttaagct ggttgctgaa agatgatcgc gtgacgtcgg
    37861 tattgattgg tgccagccgc gcggagcaac tagaggagaa cgtgcaggcg ctgaataatc
    37921 tgacatttag caccgaggag ctggcgcaga ttgatcagca tatcgccgat ggcgagctga
    37981 atctgtggca ggcgtcgtcc gataaatgac ctgttaataa cgggcgcgcg aaccgcgccc
    38041 gaatcagatc agtgattatg acgagtcagt cggtcaagat agcccatcac aaatgcagaa
    38101 agcacaaacg tcagatggat aatgacgtac cacatcagtt tgttatccgg gacatttttc
    38161 gcatccataa agacgcgcag taagtgaatg gaagaaattg ccacaatcga cgctgctact
    38221 ttgtttttca gcgacgttgc gtccattttc cccagccact tcagcttctc tttgttctcg
    38281 gagatatcca gctgtgagac gaaattctca taaccggaaa acatcaccat caccagcaaa
    38341 ccgccaacca gcgtcatatc caccagcgac agcaacacga ggatcaaatc tgattccgcc
    38401 atcgagaaga tattcggcag tacgtgaata atctcctgga agaacttcag cgccagggca
    38461 actaacgcca gcgaaaggcc aaagtacacg ggggcaagca gccagcgaga agcatacatt
    38521 gcattttcaa gaaaacgttc catacattcc tgtcgtgtag ttaaaacggg caacagtata
    38581 tcgcaattga gtaaacagca cgcaacagca aatcaacata tacgttaaac ctttgtcgca
    38641 taattctgag gtttagggat cttcctggtt cccttatcaa cgcatcgaga gcgtctaccc
    38701 ttaaaggacg ttgaacctga agggagaaaa acgatgtctc atttaaaaga cccgaccacg
    38761 cagtattaca ctggtgaata tcccaaacag aaacaaccga cgccaagcat ccaggcgaag
    38821 atgacaccga taccggattg cggcgagaaa acctatgttg gtagcggtcg cctgaaagat
    38881 cgtaaagcac tggtgacagg gggcgattcc ggaataggtc gcgctgccgc catcgcttac
    38941 gcgcgtgaag gggctgacgt ggcgatcagt tatcttcccg tggaagaaga agacgctcag
    39001 gatgtgaaaa agatcattga agaatgcgga cgcaaagccg ttctgctgcc aggcgattta
    39061 agcgatgaga aattcgcccg ttcgctggtt cacgaagcgc acaaggcgtt aggcgggctg
    39121 gatattatgg cgctggtcgc cgggaaacag gttgccattc cggatattgc agacctcacc
    39181 agcgaacagt ttcaaaagac ctttgccatt aacgttttcg cgctgttctg gctaacccag
    39241 gaagcgatcc ccctgctacc gaaaggtgca agtgtcatca ccacttcgtc aatccaggca
    39301 taccagccaa gtccgcattt actggactat gcggctacga aggcggcgat tctgaactac
    39361 agccgtggcc tggcaaaaca ggtcgcggag aaaggtattc gggtgaatat tgtcgcgcca
    39421 ggccctatct ggacggcact gcaaatttcc ggcggacaaa cgcaggataa gatcccgcag
    39481 tttggtcaga aaacgccgat gaagcgtgcg gggcaaccgg cggaactggc ccctgtatat
    39541 gtttatctgg caagtcagga gtcgagctac gtcaccgcag aagtgcacgg cgtgtgcggc
    39601 ggcgagcatt taggttaaaa aaatgcccgg ttgtgaaaag caaccgggca tcattgtgaa
    39661 ttcactctta cctgatgtgg cgaaaacgcc ttatccgcct acaaacgcat gaagattcaa
    39721 catgttgcaa tagattttgt aggcctgata agcgtagcgc atcaggcatt gagcacctaa
    39781 tgccggatgc gtcgtgaacg ccttatccgg cctacaaacg cacgaagatt caacatgttg
    39841 caatagattt tgtaggcctg atgagcgtag cgcatcaggc aattctactt tacttcgctt
    39901 tggcggtttc ttcgccgacc agacctatct tcaggtaacc cgcctgatgc agcgtatcca
    39961 ttaccttcat caacgtctcg taatcgacgg ttttgtccgc tcggaagaag atggtggtgt
    40021 ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat tgtttcatcg gtgaccagat
    40081 cgttaccgat aaacatcgag ttgtctgcct tcaccgacag ataaaccggt ttttccgggc
    40141 gcggctgcgg cgtgctggta gaagcaggca agttcacctt cacatctacc gtcgctaacg
    40201 gtgccgccac cataaagata atcagcagaa ccaacatcac gtcgataaac ggcgtcacgt
    40261 tgatatcatg catttcgccg ttatcgtcga ggttttcgtt aagatgcatt gccatcggac
    40321 attatcctgc gcgtaatttt tgtgcgacac gaaccggatg cgcagcggcg ctggcttcca
    40381 gatccaggtc acggctttgc agcaacaata cctgcgctgc aacatcaccc agcatcgctt
    40441 taaagccgcc aatctggcgt gcaaatacgt tatagataac gaccgcagga atcgctgcca
    40501 cgaggccgat tgccgttgct aacagagctt ctgcgatacc cggcgcaacg actgccaggt
    40561 tagtggtctg cgtttgcgcg ataccaataa agctgttcat gatgccccag accgtaccaa
    40621 acagaccaac gaacggagaa atcgcgccga tggttgccag atagccgtta ccgcggccca
    40681 tttgacgacc cactgcggcg acccgacgct ccagacggaa gctggtacgt tctttaatac
    40741 cttcgttatc gtcgctgcct tctgacagtt ccagctcatt ctgcgcttca ttgagcaaat
    40801 gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc gttggcctgg tttaaggaac
    40861 gtgcttcagc cagcagttgc tgctcgcgct taaggcgacg cttctgattg aagaactcta
    40921 cgctcttact gaagaagatt gcccaggtga ctacagaggc caaaataagc ccaatcatca
    40981 cgcacttaac gacaatatcg gcgtactgat acatacccca gacggaaagg tccgtctgca
    41041 ttaaattatt acccactgtg tatctccagg acgcaagtca caaaatctgc gcataataat
    41101 atcaaaacga cgtcgaattg atagtcgttc tcattactat ttgcatactg ccgtaccttt
    41161 gctttctttt ccttgcgttt acgcagtaaa aaagtcacca gcacgccatt tgcgaaaatt
    41221 ttctgcttta tgccaattct tcaggatgcg cccgcgaata ttcatgctag tttagacatc
    41281 cagacgtata aaaacaggaa tcccgacatg gcggacaaaa agcttgatac tcaactggtg
    41341 aatgcaggac gcagcaaaaa atacactctt ggcgcggtaa atagcgtgat tcagcgcgct
    41401 tcttcgctgg tatttgagag tatggaagcc aaaaagcacg cgacgcgcaa tcgcgccaat
    41461 ggcgagttgt tctatggacg gcgcggaacg ttaacccatt tttccttaca acaagcgatg
    41521 tgtgaactgg aaggtggcgc aggctgcgcg ctatttccct gcggggcggc agcggttgct
    41581 aattcgattc ttgcttttgt cgaacagggc gatcatgtgt tgatgaccaa caccgcctat
    41641 gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac tgggcgtaac gacatcgtgg
    41701 tttgatccgc tgattggtgc cgatatcgtt aagcatctgc agccaaacac taaaatcgtg
    41761 tttctggaat cgccgggttc catcactatg gaagtccacg acgttccggc gattgttgcc
    41821 gccgtgcgca gtgtggtgcc ggatgccatc attatgatcg acaacacctg ggcagccggt
    41881 gtgctgttta aggcgctgga ttttggcatc gatgtttcta ttcaggccgc caccaaatat
    41941 ctggttgggc attcagatgc gatgattggc actgccgtgt gcaatgcccg ttgctgggag
    42001 cagctacggg aaaatgccta tctgatgggc cagatggtcg atgccgatac cgcctatata
    42061 accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc aacatcatga aagcagtctg
    42121 aaagtggccg aatggctggc agaacatccg caagttgcgc gagttaacca ccctgccctg
    42181 cctggcagta aaggacacga attctggaaa cgagacttta caggcagcag cgggctattt
    42241 tcctttgtgc ttaagaaaaa actcaatgat gaagagctgg cgaactatct ggataacttc
    42301 agtttattca gcatggccta ctcgtggggc gggtatgaat cgttgatcct ggcaaatcaa
    42361 ccagaacata tcgccgccat tcgcccacaa ggcgagatcg attttagcgg gaccttgatt
    42421 cgcctgcata ttggtctgga agatgtcgac gatctgattg ccgatctgga cgccggtttt
    42481 gcacggattg tataacattg ccacttttgg acaattttgc agacatttta ttgtgaaaag
    42541 tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga ttcaggagta caataggcag
    42601 ataaaggctt aaacgctgtt ccacaggaaa gtccatggct gttattcaag atatcatcgc
    42661 tgcgctctgg caacacgact ttgccgcgct ggcggatcct catattgtta gcgttgttta
    42721 ctttgtcatg tttgccacgc tgtttttaga aaacggcctg ctgcccgcct catttttgcc
    42781 aggcgacagc ttgttgatac tggcaggcgc attgattgcc caaggggtta tggattttct
    42841 gcctacgatt gcgattctga ccgccgcagc aagtctgggc tgctggctaa gttatattca
    42901 ggggagctgg ttagggaata ccaaaacggt gaaaggctgg ctggcacagc ttcctgctaa
    42961 atatcaccag cgcgccacct gcatgtttga ccgccacggt ctgctggcgc tgctggctgg
    43021 acgttttctt gcatttgtcc gtacgctgct gccaaccatg gcgggaattt ccggtctgcc
    43081 aaaccgccgc ttccagtttt tcaactggtt aagtggattg ctgtgggtca gcgtggtaac
    43141 cagttttggc tatgccttaa gtatgattcc gttcgttaaa cgccatgaag atcaggtaat
    43201 gacgttcctg atgatcctgc caattgcctt gttaaccgct ggcttgttag gcacgctgtt
    43261 tgtggtgatt aaaaaaaaat actgtaacgc ctgacgattt tccccgttcc cggctgctgt
    43321 accgggaacg tatttaattc ccctgcatcg cccgcattct tgccgcatct tcccccggcg
    43381 tcacaccgaa gtaacgttta aactcacggc taaattgcga tgcgctttca tagccgacgc
    43441 gcatcgctgc tgcgctggcc ttcatgccgt catggatgat catcatccgc gccttatgca
    43501 gacggtaatt cttcaaatac tgcaacggcg aggtgctggt gacagactta aaattatggt
    43561 ggaacgccga tacgctcatg ttggcttctg ccgccagttg ctcgacgctc aggttttcgg
    43621 tgtatttatt ctcaattcgt ttcagcacgc ggctaatcag actgaagtga gtctggcgac
    43681 tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag cacgtagtac agaatttcgc
    43741 ggatgatctg tttgccgaga atacgcgcat ccagtggtcg ctccatcacg tcgagtaacc
    43801 gctccgccgc gcataaaatc tcttctgata acgtggcgga gttaatcccg ctggctgcca
    43861 tcgacggctg gaaatgctca tcttcgccaa tgtccatcaa cagttcctgt aactgcaaaa
    43921 tatcgacatt gagacgcaac cctgccagcg gcacctctga cgtggcatag gtttcgcact
    43981 caaacggcaa cggcaccgtc agcagcaggt attcattggc atcataacga aacacgcgtt
    44041 cattgatata accgatttta tgcccggaaa agagaattat gatgccaggc tcgtacatca
    44101 ccggtgtacg tgcgaaaggc gtctcgccat acaacaaacg cacatcgggc aacagttctg
    44161 acaaactatt ttctttattt ttcagtttat taactttatc cgccagcaag cgacaaatct
    44221 cttcacgttt catatcgcgt aatttcttag gaataatgcg gcaatttgat tgtgcgcaat
    44281 tttgtagcat ttctccagca ctctggagaa ataggcaaga cattggcaga aatgagcatt
    44341 gagagccagg gcgctggcga tcacaatgaa aaacatcagg cagatcgttc tctgccctca
    44401 tattggccca gcaaagggag caagtaatga acaactttaa tctgcacacc ccaacccgca
    44461 ttctgtttgg taaaggcgca atcgctggtt tacgcgaaca aattcctcac gatgctcgcg
    44521 tattgattac ctacggtggc ggcagcgtga aaaaaaccgg cgttctcgat caagttctgg
    44581 atgccctgaa aggcatggac gtgctggaat ttggcggtat tgagccaaac ccggcttatg
    44641 aaacgctgat gaacgccgtg aaactggttc gcgaacagaa agtgactttc ctgctggcgg
    44701 ttggcggcgg ttctgtactg gacggcacca aatttatcgc cgcagcggct aactatccgg
    44761 aaaatatcga tccgtggcac attctgcaaa cgggcggtaa agagattaaa agcgccatcc
    44821 cgatgggctg tgtgctgacg ctgccagcaa ccggttcaga atccaacgca ggcgcggtga
    44881 tctcccgtaa aaccacaggc gacaagcagg cgttccattc tgcacatgtt cagccggtat
    44941 ttgccgtgct cgatccggtt tatacctaca ccctgccgcc gcgtcaggtg gctaacggcg
    45001 tagtggacgc ctttgtacac accgtggaac agtatgttac caaaccggtt gatgccaaaa
    45061 ttcaggaccg tttcgcagaa ggcattttgc tgacgctaat cgaagatggt ccgaaagccc
    45121 tgaaagagcc agaaaactac gatgtgcgcg ccaacgtcat gtgggcggcg actcaggcgc
    45181 tgaacggttt gattggcact ggcgtaccgc aggactgggc aacgcatatg ctgggccacg
    45241 aactgactgc gatgcacggt ctggatcacg cgcaaacact ggctatcgtc ctgcctgcac
    45301 tgtggaatga aaaacgcgag accaagcgcg ctaagctgct gcaatatgct gaacgcgtct
    45361 ggaacatcac tgaaggttcc gacgatgagc gtattgacgc cgcgattgcc gcaacccgca
    45421 atttctttga gcaattaggc gtgccgaccc acctctccga ctacggtctg gacggcagct
    45481 ccatcccggc tttgctgaaa aaactggaag agcacggcat gacccaactg ggcgaaaatc
    45541 atgacattac gttggatgtc agccgccgta tatacgaagc cgcccgctaa gctttttacg
    45601 cctcaaactt tcgttttcgg gcatttcgtc cagacttaag ttcacaacac ctcaccggag
    45661 cctgctccgg tgagttcata taaaggagga acgtatggct aatccaaccg ttattaagct
    45721 acaggatggc aatgtcatgc cccagctggg actgggcgtc tggcaagcaa gtaatgagga
    45781 agtaatcacc gccattcaaa aagcgttaga agtgggttat cgctcgattg ataccgccgc
    45841 ggcctacaag aacgaagaag gtgtcggcaa agccctgaaa aatgcctcag tcaacagaga
    45901 agaactgttc atcaccacta agctgtggaa cgacgaccac aagcgccccc gcgaagccct
    45961 gctcgacagc ctgaaaaaac tccagcttga ttatatcgac ctctacttaa tgcactggcc
    46021 cgttcccgct atcgaccatt atgtcgaagc atggaaaggc atgatcgaat tgcaaaaaga
    46081 gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc catcacctgc aacgcctgat
    46141 tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa cttcatccgc tgatgcaaca
    46201 acgccagcta cacgcttgga acgcgacaca caaaatccag accgaatcct ggagcccatt
    46261 agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt cgcgatctgg cagataaata
    46321 cggcaaaacc ccggcgcaga ttgttatccg ctggcatctg gatagcggcc tggtggtgat
    46381 cccgaaatcg gtcacacctt cacgtattgc cgaaaacttt gatgtctggg atttccgtct
    46441 cgacaaagac gaactcggca aaattgcaaa actcgatcag ggcaagcgtc tcggtcccga
    46501 tcctgaccag ttcggcggct aacgtgcaaa ttctcccggt ggcggtaatg ttccgctacc
    46561 ggacttttca gaaatcattt attcccctcg cgtcccgccc gttgttactc ttccttgttc
    46621 aggaatgcca aatataagga catcatcatg cagagccgga agctcttaaa agaacaactc
    46681 atctatatcc gggataaacg caacggagag gtgaaaaaca gatgaaaata atacttctgt
    46741 ttttagcagc cctggcaagt tttaccgtac acgcgcagcc tccttcacag accgtagaac
    46801 aaacagtccg gcagatttat cagaactata aatcagatgc cagtacgcct tatttcggtg
    46861 aaaccggaga gcgggcgata acttcagcgc gtattcaaca ggcgcttacc ctgaacgaca
    46921 atcttacact accgggcaat attggctggc tggattatga tccggtttgt gattgtcagg
    46981 attttggcga tctggtgtta gaaagcgtag cgataaccca aactgacgtc gatcatgccg
    47041 atgccgttgt gcgctttcgt atctttaaag atgataaaga aaagaccaca cagacactga
    47101 aaatggtggc ggaaaatggt cgttgggtca ttgatgatat tgtcagcaat catggcagcg
    47161 tcttacaagc agttaatagc gagaatgaaa aaacgctggc cgctttagct tcgttgcaga
    47221 aagaacagcc agaatccttt gttgctgaac tctttgaaca tattgctgat tatagctggc
    47281 cgtggacgtg ggtggtttcc gactcttacc gccaggcggt taatgccttc tataaaacca
    47341 ccttcaagac ggccaataat cccgatgaag atatgcaaat agaacggcaa tttatttacg
    47401 acaatccgat ctgttttggc gaagagtcgc tattttcacg cgttgatgaa attcgagtcc
    47461 tggagaaaac cgccgattcc gcccgcattc atgttcgttt tacgctgacc aatggcaaca
    47521 atgaagagca agagctggtt ttacagcggc gcgaaggtaa gtgggaaatc gctgatttta
    47581 tccgcccgaa cagcggcagc ctacttaagc agattgaggc aaaaaccgcc gccagattaa
    47641 agcaatgagc tgaattaaat aacaattagc cggaacaata aataaaaggg aacactatat
    47701 gaaaacgatt ttcaccgtgg gagctgttgt tctggcaacc tgcttgctca gtggctgcgt
    47761 caatgagcaa aaggtcaatc aactggcgag caatgtacaa acattaaatg ccaaaatcgc
    47821 ccggcttgag caggatatga aagcactacg cccacaaatc tatgctgcca aatccgaagc
    47881 taacagagcc aatacgcgtc ttgatgcaca ggactatttt gattgcctgc gctgcttgcg
    47941 tatgtacgca gaatgataaa aaaatccccg gcagcatgtc agttgccggg gatttttttt
    48001 aacgtccaac cgccgcttta gggcgtttct tcgcaccagc attcaccgga cgagattgcg
    48061 tagacgacgc tttttttgcc gtagcaggcg tctgacgctg agtcgccatc ggcgtatgtt
    48121 tagtcaacgc cggacgggta ttgcggttct ggcgacgagc ttcacgcatc tcttcaatgg
    48181 ttggcgcagg cactaagcaa tcgcgacggc tgccaatcag atgctttttg cccatcgctt
    48241 ccagcgcctg gcggattaac ggccagtttg ccggatcgtg gtaacgcaac aacgctttat
    48301 gcaaacgacg ctgtttgtcg cccttcggta cgaagacgcc ttcgctctta taaccaatct
    48361 tcgccagcgg ttttttcccg gtgtaataca tggtggttga gttcgccagc ggcgatgggt
    48421 agaagttctg tacctggtcg agacggaaac gatgcttttt cagccacagc gccaaattca
    48481 ccatatcttc atcacgcgta ccggggtgcg cagagatgaa atacgggatc agatactgct
    48541 ctttacctgc ctgtttcgag taggtatcga acagctcttt aaagcggtca tagctgccca
    48601 tgcccggctt catcatcttc gacaacggcc cttcttcggt atgttccgga gcaatcttca
    48661 gataaccgcc gacgtgatgg gtcgccaact ctttgatata gcgcggatct tccacggcga
    48721 tgtcataacg tacgcctgag gcgataagga tttttttaat gcctttcaga tcacgcgcac
    48781 ggcgatagag gttgatcgtc ggttcgtggt tagtgtccat gtgcggacaa atatccggat
    48841 aaacgcacga caaacggcga caagtttgtt cagcgcgtgg cgatttgcag cgcaacatat
    48901 acatgttggc agttggccca ccgagatcgg aaatcacgcc cgtaaaacct ggaacggtgt
    48961 cgcggatcgc ttcgatctca ttaatgatcg aatcttccga acggctctga ataatgcgcc
    49021 cttcgtgctc ggtaatagaa cagaaagaac agccgccaaa gcagccgcgc ataatgttga
    49081 ccgaaaaacg gatcatttcg taagccggaa tacgggcatt gccataggcc ggatgtggca
    49141 cgcgcttgta cggcagcgca aaaacgctgt ccatctcttc ggtagaaagc gggatagcag
    49201 gcgggttgat ccacacatag cggtcaccgt gtttttgcat caatgcgcgg gcgcagcctg
    49261 ggttggtttc gtggtgcaga atgcgcgaag catgggcgta cagcacttta tcgcccttca
    49321 ctttctcgaa agaaggcagc aatacgtagg ttttttccca cggtttctgg cgcggtggct
    49381 gcacgattac agctttcgct tcctgctttt tcggcgcaac aggtttgtta tccgcacacg
    49441 gcaaatcttc gccatacgga tgcgggattg ggtcgatttt ccctagggta tcaagacggg
    49501 tggaatccac accgctccag ccaggcagcg cctcttttac gataattgcg gtattacgca
    49561 catcgcggat ttcactaatc ggctcgccca tcgccagacg atgcgctact tccaccagcg
    49621 gacgttcacc gttaccaaac atcagcatgt cggctttcga atctaccaga acggaacggc
    49681 gcacggtatc ggaccagtaa tcataatgcg cggtacggcg caggctagcc tcaataccgc
    49741 cgaggatcac cggcacatct ttccacgcct ctttacaacg ctgggtataa accagcgtgg
    49801 cgcgatccgg tcgcttaccc gcgacattat ctggcgtgta ggcatcatca tgacgtaaac
    49861 ggcgatcggc ggtatagcgg ttgatcatcg aatccatgtt gccagcagta acaccaaaaa
    49921 acagattcgg tttacccaga cgcataaagt cgtctttgct gctccagtcc ggctgggcga
    49981 tgatcccgac gcgaaagccc tgcgcttcca gcatacgacc gcaaatcgcc atcccgaagc
    50041 ttgggtgatc gacatacgcg tcgccagtaa ccaaaatgat gtcgcagcta tcccagccaa
    50101 gttgatccat ctcttcacga gacatcggca aaaacggtgc cggtccaaaa caggcggccc
    50161 agtactgcgg ccaggagaac aggtcgcgat ccggttggat cagggagata gagctcattt
    50221 tgcttccaga aatgataaaa aaataatcaa aggccgggga ttataagccg gaacgaaaga
    50281 gaaatcgaaa ggtattccat actcgccctc ctcgggcgag tatgaagatt acggtaccgg
    50341 attgaccaac agttgcccaa tcgagccacg gtccgccatt tccagcgtct gactgttgaa
    50401 gtagaacggg aagtgcgccc aggaaggctg accgaaataa acaagcagct ccacttgtcc
    50461 gtcaacccaa acggtatctt tccagcctct gtcttccggg aacggcatcg cgccattcac
    50521 gttacggatc tggaacatta cgccttcaat atggaacgct tgcggctcgt ccgcgcgtac
    50581 cgtccagcgt tcccacgttc cttgctgcgc ggtgacatca atacggttga cgtcccacag
    50641 ctgtccatta atacccgggt catcacccag actgatatcg cgactgcgaa ttggcgaacc
    50701 agccatgatt tcagttggca gcaagcgcat cggaagactg tctgtgacca gcggcagaag
    50761 gccggttggg cgcagcgtta gcaccaaggt agaaaccaga atactggatg gctcaaagaa
    50821 gccgcgaata cgatcaacaa tgctcgccgc ttcaccacag gtgatcgaca cttcatcgcc
    50881 gttgctcata tccaccagaa tctcgcggcg ctcgcccggt gccagcgaaa gttgcttcac
    50941 cgatacagga gcaggcagga atccctgatc gccagaaatc acatgtaacg ggcgaccatc
    51001 gctcatctgt agttgatagc gacgagagtt cgacgcgttc agcagtcgca agcgcaccca
    51061 gccacgcgag acttcaacgt acgggctttg tacaccgtta accagcagcg tatcaccaac
    51121 aaagccgccg cttcccggtt cgttgtattc tggcgtacca aagttatcca gccgtttatc
    51181 ctggataatg accggaaaat catccacacc ataatggttg gggataggca gcgacttgct
    51241 gacttcatct tccaccagcc acattccggc aaggccgtta tagacctgct gagccgtgcg
    51301 gttgggagta ttggcgtgat accacagagt agctgcgttc tggcgaatag gcagtacggg
    51361 tgcccagtca gcgtttggcg acatcatccg tgccggaccg cccatcagcg ggcctggtac
    51421 ctgtagcccg gccaccgtca ttgagacatt ttctgtcagg cggttgctgt aaataagctt
    51481 aacatcgtcg cccttccaga cgcggatagt cggccccagg taacgaccat tgattcccca
    51541 gaccgacgcg cgtgtccctg gcgtaaatga ccagtgcgca cgttgtacag tcataaacag
    51601 cggttgccca cggcgagatt caagtagcgg cggaacgggt agcggttgct gttgcccggc
    51661 tgcgctggcc ttcaggggaa cagcgcctgc acaaagtgca atccccgatg cctgaatgaa
    51721 ctgacgccga ctgagtgaca tatttgctcc atgtaaaact ggctaaagta ataccgtgca
    51781 ggaaagtgtt ccccgcaaaa atacagattg ccgacttaaa cttttccggc ggcttcgcgt
    51841 tctgcgactt ctttatcgag ctcggcgatt ttttgttcca ttatcgaacg acaatgggca
    51901 gccagctcac gaacctgatc tttgccatac tgactgacgt caattggcgg cagcatttcg
    51961 acaatcacca gaccgttgtg cagtcgatta agattaatct tattcgaagt tgtagagacg
    52021 cacacgggaa taatcgggac gcccgccgca attgccgcgt gaaatgctcc agtcttgaac
    52081 ggtagcaggc cgcgaccacg gctgcgggtt ccttccggga acatccagat ggaaatacgg
    52141 cgttttttga aatgattcac gacttccgca atggtgccgt gagctttagt gcgattgttt
    52201 ctgtcgatca ataagttgcc ggttaaccag tacaactgcc cgaagaaggg gatccacagc
    52261 aagctctttt tacctaccgt caccgtcggc ggttgcacaa tgttcgatgc tgtcaccatg
    52321 tcatagttgt tctggtggtt agcgatatag atagcattgc cgtagctttc agcatctgca
    52381 ggtttgcggc actcaacttt caggccgaac agtggcgcaa gacggccaaa catatgccca
    52441 aaggtggcca catgtttcgg gttacgcggg ctgaaaaggc agtaaatgga gccgaataca
    52501 cagactaaga tgctgtaaat cacggtaata ataagacgaa agatatatag cataacaccc
    52561 tctgaaggtc tgagagactg gcattgtacg tcacctgaaa tcaggttagg gcattgttaa
    52621 aagccgctct ctggaaaagc gtattgttaa tcgcaacgca tgaataaaca acggtttcaa
    52681 gggaattttt atcgtttccg ggggttgaat acgctgccgg atgacgactt aacgtttcat
    52741 ccggcgttcc ttgctagcgg gaggaaacag cgccctcccc ggcatattac tcttcgctat
    52801 caccgctgct ggcacggcga ggagagtcga tctcaacacg atcgatacgc tgcaaaccgc
    52861 gcatcaacgt accgcggcgt ccacgttcgc cagtgacttt ctgtagctct tccggacgca
    52921 gtttaatttt gcgtttccca acatgaatgg tcagcgtgct ttgcggcggc agaacgtaca
    52981 gttgcgccag accatcctcg ccacgcgcgg cttctgccga tggaatgttg ataatcttgt
    53041 tgcctttgcc cttcgacagc tgcggcagat cgcttaccgg gaacatcaac atacggcctg
    53101 cctgagtgat tgccagcagc atatcggaag catcttcaat caccaccggc ggcataacat
    53161 gggcattttc cggtaaggtg atcaaagcct tacccgcacg gttacgcgct accagatcgt
    53221 taaaggtgca gacgaaaccg taacccgcat cggaagccat cagcagtttc tgatcgtcgc
    53281 tttccatcag catatggtca acggtcgccc caggcggcaa cgttaatttg ccggtgagtg
    53341 gttcgccctg accacgcgcc gacggcagcg taatcgggtc gatggcatag ctacgaccgg
    53401 tggaatcaac aaacactacc ggttggttac tcttaccttt caccgccgct ttgaagctat
    53461 cacccgcttt ataattcagg cccggcgcgt cgatatcatg gcctttagcg ctgcgtaccc
    53521 agcccatctg cgacagcaca atggtgacag gttcagacgg cagcatgtcg tgctcgctca
    53581 tcgctttcgc ttcttcgcgt tcctgcaacg gcgaacgacg atcgtcaccg taggcttgcg
    53641 cgtctgcctg cagttctttc ttcagcaggt tattcatttt acgctcggaa gccaaaatgc
    53701 cctgcaactg gtcgcgctct ttttccagct cactctgctc accgcgaatc ttcatctctt
    53761 ccagtttggc aagatgacgc agtttcagtt cgaggatcgc ttccgcctgg gtttccgtaa
    53821 ggccaaaccg cgacatcagc gccggtttcg gttcatcttc gttacgaatg atctcaatca
    53881 cttcgtcgat gttgagaaac gccaccagca aaccttccag gatatgcagg cgcttgagga
    53941 ctttctccag acgatagttc agtcggcggc gcacggtatc gcgacggaac accagccatt
    54001 cggagaggat ttccagcagg tttttcaccg ccggacgacc atccagaccg atcatattga
    54061 ggttaatgcg atagctcttt tccagatcgg tggtagcgaa gaggtggttc atcacctgat
    54121 ccatatccac gcggttggaa cgcggcacaa tcaccagacg ggtcgggttc tcgtggtcag
    54181 attcatcgcg cagatcgtca accatcggca gctttttgtt gcgcatttgc gcagcaattt
    54241 gctccagtac gcgcgcacct gaaacctgat gcggtaatgc gctgataacc accgcgccat
    54301 cttctttctt ccacaccgcg cgcatacgca ctgaaccacg tccgttctcg tagattttac
    54361 gaatctcggc gcgcgaagtg ataatttccg cttcagtcgg ataatccggc ccctgcacga
    54421 tatccagcag ctgatcgagc gtagttttcg gctggtcgat taatgcgatt gccgcctgag
    54481 ctacttcacg caggttatgc ggtggaatat cggtcgccat gccgacggca ataccggtgg
    54541 tgccgttaag caaaatgttt ggcagacggg caggtagcat tttcggctcc tgcaaagtgc
    54601 cgtcgaagtt tggcacccag tcagccgtcc cctgccccag ctcgctcaat agcagctcgg
    54661 aatatttcga caaccgggat tcggtgtaac gcattgccgc gaacgatttc ggatcgtccg
    54721 gcgcgcccca gttcccctga ccatcaacca gcggataacg gtaagagaac ggctgcgcca
    54781 tcaggaccat cgcttcataa caggcgctat cgccgtgcgg atggtattta cccagtacgt
    54841 caccgacggt acgggccgat tttttaaatt tggcgctggc attcaggccc agttcagaca
    54901 tcgcatacac aatgcggcgc tgaacaggtt tcagaccatc accaataaac ggcaacgcac
    54961 ggtccatgat cacgtacatg gagtagttta agtaggcgtt ttccgtaaat tcatgtagcg
    55021 caaggcgctc tgccatatcg ctcattaatt ctgattcctc aacttattcg ccagccttca
    55081 aaggggcaat attgccggag atactacatt atctgccgcg tcgagtcaca ggagatcagc
    55141 gtgaatagcg ccgccggacg tgctccgacg gcgataaagg catcaatgtg ccttgatata
    55201 caactctttg ctgtagactt gatcggcata gagggcagca caaggttgcg cccatgccac
    55261 accactcggc atgggggtcc gctccgggcg attcgagaag ttgaggttat gcgagtccag
    55321 ggaagcctaa agcatcaaag tagtcgactg taagtatacg gttcagccca gtttagctgt
    55381 gtctccacca gtgccccgcg cctcctgcca taagtgcggc ttgctcatgg atatccccga
    55441 gacggcttac ggcgcaccgg ataataaatt tcgcgtatac atggtattcc ttaccggaga
    55501 aattataagc ccaacaacaa tacgcctgtg acattgttcg ctcaaactta tcgcgagtga
    55561 tttggctcac actccggtaa agattcccca tcacgccaac ggcacataaa catccgtttc
    55621 cagtcgctgc tctgtcaccc cttcggcaag attggtgtag tgaaacagaa tcggtgcttt
    55681 acgcattttc tcgccgcttg caggcagcca gtggcgaata atgccccata tcgtgtgtga
    55741 aatatcgtcc agctcgccaa cgtggcgggc cacggcataa cgtccaccgg taagttcacc
    55801 attgctcaca ccataacgat tatcgggaat cagttcgcta acgctgccgc agatatcaaa
    55861 gcgaaacgct tccggtgcgg tggtggcagg ggcgtcccag gcgacgccaa aagtctggct
    55921 ttggttaaca ggagaaagcc ccgtctcttt acgccacatg ataaatttcg ccacactgta
    55981 gttgagcaac tcagggctac agcggtgcgg caacatcgcc acagggattg atgggaaatc
    56041 gataatgttt acatccagtg tcaggtttgt cattttgcct ccctgcgaaa ttattcccgg
    56101 gaacgcgtcc cgggagcggt aacaattacg gatttacttt gcggatctgt ttgacgtcaa
    56161 tttcaacaga attccagtct ttatcgactt caccctgaat ctcaaccgta tctttcggcg
    56221 tcaccgtcac accgttccag cgtttgtgat cgatatcaac attgatagta ccgctggcat
    56281 ctttaaacac gtagagatcg tcagagatgc gttcaacgat attgccgcgc agggttaccc
    56341 aggtgtcgtc acgcagggat tttgcgcttt ctacagtcgt tacgctgccg ttcggcccct
    56401 gaaatcctcc ggcctgactt tgcgttaccg atgggccaga aaaaccgccc tgctctgctg
    56461 ccatcaccgg tgcactgcac agggccatta ctgcgattac tgctgcgaat tttttcatgt
    56521 ttattactcc ctttaatgtc tgtttccgag catttaacaa gatagtcctt aacaacttct
    56581 taagggaaaa aaataaaatt taatgctgta cagagcgcgt tacaacacgg tttactggca
    56641 gcaaatacgg ttatcgcagg gatgaaaaaa tgcgaatttt actgatagaa gatgacatgc
    56701 tgattggtga cggcatcaaa actggcctta gtaaaatggg ttttagtgtc gactggttta
    56761 cacaaggtcg tcagggaaaa gaggcgcttt atagcgcacc ttatgatgcg gtgatcctgg
    56821 atttaacctt accaggcatg gatggtcgcg atattttgcg cgaatggcga gagaaaggtc
    56881 agcgtgagcc ggtactgaac ctgaccgcgc gtgatgcgtt agcggaacgt gtagaagggc
    56941 tgcgtctggg agctgacgat tatctgtgta aaccttttgc gttgatagaa gtcgccgcca
    57001 ggctggaggc tctgatgcgc cgaaccaacg gccaggccag caacgagctg cgccacggta
    57061 acgtcatgct cgaccccggc aaacgtatcg ccacgctggc tggcgaaccc ttaacactga
    57121 aaccaaaaga atttgccctg ctggaattac tgatgcgtaa cgctggtcgg gtactgccgc
    57181 gcaaactgat tgaagagaaa ctgtatacct gggacgaaga ggtcaccagt aatgccgttg
    57241 aagtgcatgt gcatcatctg cgacgcaaac tcggtagtga ttttattcgt accgtgcatg
    57301 gtattggcta cacattaggt gagaaatgaa atttacccaa cgtcttagtc tgcgcgtcag
    57361 gctgacgcta atctttttaa ttctggcctc ggtgacctgg ctgctttcca gctttgtcgc
    57421 ctggaaacaa acaacggata acgtcgatga attgttcgac acccaactga tgctgtttgc
    57481 caaacggtta agtacgctcg atctcaacga aatcaacgcg gcggatcgca tggcacagac
    57541 gccaaataaa ttaaaacacg gtcatgttga tgacgatgcg ctgacctttg ccatctttac
    57601 ccacgacggc agaatggtcc ttaatgatgg cgataacgga gaagatattc cctatagcta
    57661 tcaacgggaa ggttttgctg acgggcaact ggtcggtgaa gacgatcctt ggcgttttgt
    57721 ctggatgacc tcacctgatg gcaaatatcg catcgttgtt ggccaggaat gggaataccg
    57781 tgaagacatg gcgctggcga ttgttgccgg gcaattgatc ccgtggctgg tcgcactgcc
    57841 gattatgtta atcatcatga tggtactact gggtcgtgaa ctcgcgccgc tgaacaaact
    57901 ggcgctggca ctacgtatgc gtgaccctga ctcggaaaaa ccactaaacg cgactggcgt
    57961 acccagcgaa gtgcggccac tggttgagtc gctaaatcaa ctgttcgccc gcacacatgc
    58021 gatgatggtt cgagaacgac gctttacctc cgatgcagct cacgaactac gtagcccgtt
    58081 aacagcgctg aaagtgcaaa ccgaagttgc gcagctctct gacgatgatc cgcaggcgcg
    58141 gaaaaaagca ctgctccaat tacattccgg gatcgatcgc gctactcgtc tggttgatca
    58201 actgctcacg ctatcgcggc tggactcact ggataacctt caggacgtcg cggagatccc
    58261 gcttgaagat ctcctacaat cgtcggtgat ggatatttac cacacggcgc agcaggcgaa
    58321 aattgacgtg cgactgacac tcaatgccca cggcatcaaa cgcaccggac aaccgctatt
    58381 gctaagtttg ttggtgcgaa atttgctgga taacggcccc ggtgtgacac cagaggcact
    58441 ggcgcgaatt ggcgaacgct tctatcgccc acccggacaa accgctaccg gcagtggact
    58501 tgggctatcg attgtccagc gaatcgccaa gctgcatgac atgaatgttg agtttgggaa
    58561 tgcggaacaa ggtggatttg aggcgaaggt aagctggtaa cttctctgtc tatgagagcc
    58621 gttaaaacga ctctcataga ttttactaat agcaaaatat aaaccgtccc caaaaaagcc
    58681 accaaccaca aaccagacaa ataacgagcg agaatacata tagccaaaac tcgaacattt
    58741 cccgcataaa gagttttctt aagataagaa taataagtgg cgtaagaaga aaaaatgctg
    58801 caatcattgc ttgcaatata cgatcttcgc ctgaaacgga aggtatactg caaatattgc
    58861 ctcccgactc aagcatccag tcatacttat tgaaagcaaa aaaagaatct attaaccaat
    58921 aagtaagcat tatatatagc aatgcttcaa atattgtttt aattttctgc tttaacatat
    58981 atcagtacgc tcataaaaat ccatttataa ttatcaacat gacacgttga tattttcatc
    59041 ttttacttat tcaaagccat attttcaatc cccaaaaaaa ccgaaccaaa cgtgtaaatt
    59101 attccggtgc gagtaagaat tatgaggaat ggctatcagt attgtcattt tcagaaaata
    59161 tttatcctgc atcggtgagt cagagctaga tcagactttt gctaaattcg caaaagactt
    59221 tgcacatttt gctaatttca cagtaccgct ctgtgacgta ctatagtcga caaacgtctc
    59281 accttgagat taaaaaatga gcaacatcct gattatcaac ggcgcgaaaa aattcgccca
    59341 ctccaatggt caactgaacg acaccctgac cgaagtcgcg gatggcacac tgcgcgacct
    59401 cgggcatgat gtccgcatcg ttcgcgccga cagcgactac aatgtcaaag cggaagtaca
    59461 aaactttctc tgggctgatg tggtgatctg gcagatgcca ggctggtgga tgggcgcgcc
    59521 gtggacagtg aaaaaatata ttgatgatgt attcaccgaa ggtcacggga ctctgtatgc
    59581 cagcgatggt cgtacccgca aagatccgtc gaaaaaatac ggttccggca gcctggtaca
    59641 ggacaaaaaa tatatgcttt ctctaacctg gaacgcacca atggaagcct tcaccgaaaa
    59701 agatcagttc ttccacggcg ttggcgttga cggtgtgtat ctgccgttcc ataaagcaaa
    59761 ccaattcctc ggtatggaac cgctgccgac atttatcgct aatgacgtga taaaaatgcc
    59821 tgatgttccc cgctatactg aagaatatcg caagcatctt gtggaaattt ttggttaact
    59881 agagctcagg ctttagaagg agttaaccat gcttaccgta atcgcagaaa tccgtactcg
    59941 tcctggtcaa catcaccgtc aggcggtatt ggatcagttt gctaaaatcg ttccaaccgt
    60001 actgaaagaa gaaggttgcc acggctatgc gccaatggtg gattgcgctg ctggcgtgag
    60061 tttccagtct atggcaccgg attctatcgt gatgattgag cagtgggaaa gcatcgcgca
    60121 ccttgaagcg catctgcaaa ccccgcacat gaaggcgtac agcgaagccg taaaaggcga
    60181 cgtgctggag atgaatatcc gtattctgca gccagggatt taatcctgcc ttgtttgccc
    60241 ggccatcctg accgggcaat gttctttcct ttaaacctca atctccgcca tgtcgccttt
    60301 ctcttgcaac cagttgcggc gatcttccga gcgtttcttc gccagcagca tatccatcat
    60361 cgcgtcagta cgctgatcgt cttcatcatc gatagtcaac tgcaccagac ggcgagtgtt
    60421 cggatcaagc gtggtttcac gcaattgcat cgggttcatt tcccccagac ctttaaaacg
    60481 ctggacgttc ggcttgcctt tcttgcgttt taattgctca agtacgccct ctttctcttc
    60541 ttccgtcagc gcgtaataaa cctctttccc gagatcaata cggtagagcg gtggcagtgc
    60601 gacgtaaacg tgaccgtgtt tcaccaacgc gcggaagtgt tttacgaaca aagcgcagag
    60661 cagcgtggca atgtgcagac catcagagtc cgcatccgcg aggatacaga ttttgccata
    60721 acgaagctgg ctcagatcgt cgctgtcagg atcgataccg atcgctaccg aaatatcatg
    60781 cacttcctgc gaggccagca cttcgtcgga agaaacttcc caggtgttaa ggatcttacc
    60841 tttcagcggc atgatcgcct gatattcacg atcgcgcgcc tgcttggcag atccgcctgc
    60901 ggagtcacct tccacgagga acagttcggt acggttaagg ccctgcgcgg tacaatcagc
    60961 cagtttgcca ggcagcgccg ggccgctggt cagcttttta cgcaccactt ttttagccgc
    61021 acgcatacga cgctgggcgc tggaaatcgc catctctgcc agcaactccg ccgcctgaac
    61081 gttctggttc agccacagga tgaaggcatc tttcaccacg cccgaaacga atgccgcaca
    61141 ctgacgcgaa gagagacgct ctttggtctg cccggcaaac tgcggatcct gcattttaac
    61201 cgacagaaca taggcgcagc gatcccagat atcttccgcc gacagcttca cgccgcgcgg
    61261 cagaatgttg cggtattcac agaactcacg catcgcgtcc aacaggccct gacgcagacc
    61321 attaacatgg gtaccgccct gcatcgttgg gataaggttg acgtagcttt cggtcagcag
    61381 ttcaccgcct tccggcagcc acagtagcgc ccagtccaca gcttcagtat cgccagcgaa
    61441 attaccgata aacggttttt ctggcagcgt cggtaaaccg tttaccgctt ccgccaggta
    61501 atcattcaga ccgtcctgat agcaccagcg ctgttcggtg ttgttgatct catctttaaa
    61561 ggtaatctca acgccggggc acagtactgc tttggccttc agcacatgcg tcaggcgtga
    61621 aacagaaaaa cgcgggctgt caaagaaggt ttcatccggc cagaagtgca cgctggtacc
    61681 ggtattgcgt ttaccgcaag tgccgacaac ctgtaaatcc tgcacctttt cgccattttc
    61741 aaaggcgatg ttataaacct gaccatcgcg gcgcacgtta acttctacgc gcttcgacaa
    61801 ggcgttaact accgaaatcc ccacgccatg caggccgcca gagaactggt aatttttgtt
    61861 agagaattta ccgcctgcat gcagacggca aagaatcagt tcaaccgccg gtactccttc
    61921 ttccgggtga atatccaccg gcatcccgcg cccatcgtca atcacttcta acgactggtc
    61981 agcatgtaag attacatcca cgcgttttgc gtgacccgcc agtgcttcat cgacactgtt
    62041 atcaataact tcttgcccca aatggttagg gcgagtggta tcggtataca tccccggacg
    62101 gcggcgaacc ggctcaagcc cggtgagtac ctcaatggca tcagcgttat aagtttgcgt
    62161 catggtttaa gttagcaatt cgagttgatc gtcagagatg gtgcagacca agaaaatcga
    62221 cgatcgggtt gaaataatct tcgaagcccg tgaatgcgtg gttgccgcct tctatgacag
    62281 tctggcggca ggaagcatag tacgccaccg cctggcggta atccagcact tcatctcccg
    62341 tctgttgcag cagccagatc aaatccggcg cttccagcgg gtcaatctgc atgactttga
    62401 gatcgtaaat atggcgtgac tctagcacat attgctgccc ggtgtagggg ttctcgttct
    62461 gaccgagata gtccgtcagc aattcaaacg ggcgtaccgc cgggtttacc accactgcgg
    62521 gcagcataaa acattgcgac aaccaggtgg cgtaatatcc ccccagtgac gaaccgacaa
    62581 tacccagcga atcgccgcca tgttccagga caatggattc cagcagttct gccgcgtcgg
    62641 aaggatacgg cggcaactgc ggaatgatca tctcaacgtc agggtgatgt tccgccagcc
    62701 agttttttaa caagctcgct tttgcagagc gcggcgagct gttgaaaccg tgtaaataaa
    62761 gaagcgtaga catcagtagc cttctgaagc ggtatcaggt tggaaacgtg tgtccgccag
    62821 gcgatgcacc tcggtggtca gcgtgccatc agcatgtaac tcgagagtac gccagccggg
    62881 cgcgatagta tccagcgtaa agttggaaca gtgcggctta aactgcacac aggtcgacgg
    62941 cgttgccagc aggcggcgac cgttccagtc gagatccagc tcctgatgaa tatgaccgca
    63001 cagcaagtat ttgacgtgcg gaaacttcgc cagcacggta tccagttcgc ccgcgttacg
    63061 cagactgtgt tgatcgagcc aactacaacc cgcaggtagc ggatgatgat gcagcagcag
    63121 caacgtatgg cgttctggcg catcggccag tttacgttcc agccactcaa gctgaaactc
    63181 gctcagctca ccgtgcggca cgccaaacac ctggctatcc agcaacagga tttgccattg
    63241 ctcaccaata aacacccgct tcgccgggga gatacccgca tcctgtaacg cgctgtacat
    63301 cgcgggctgg aaatcgtggt tgcccggcag ccagacgcag ggcgcacgaa aacttgcgat
    63361 gccttcagcg aaatgctgat aggccgcaga ggattgatcc tgcgctaaat cacctgtcgc
    63421 gacaatcagg tcgaattcgt gctggtgtgg acgaatcgcc tccagcaccg cctggtagct
    63481 ttcccaggtg tttaccccta acagggcttc gtgcttttgt gcaaacaggt gagtatcggt
    63541 aatttgtaaa atccttcctc tggcctcacc agccagagga agggttaaca ggctttccaa
    63601 atggtgtcct taggtttcac gacgctagta aaccggaatc gccatcgctc catgtgctaa
    63661 acagtatcgc aaccagtccg ctaaaaactg gttaatttga tgcttttcgt cgcgttgatg
    63721 caacttttta ttaggataat cataccgcgc tttgaagcga aaaatctgct ggcttgaaca
    63781 cacttcagcc accatcgcgt catgatacag acgcaccgtc attgacggaa ggctccagta
    63841 actgatcgcg ggcgcagtct gttctattgt caccagggta gtgtatcggg tcgattccac
    63901 aatcgtcagc cgatattgtg cgtttgccac ctgatagctt acagtttcgc cgggtgcgtc
    63961 attgcgcggt aacaaacggc gcaattgtga aaagttcatc tcgcacaggc gcatcatttc
    64021 aggaaagtca ggtgtgtaac gcttcattta tgcccactca ttttttaacg cttgatgatg
    64081 cagctgcagc cattgcaaag cgatgaccga cgctgcgttg tcgattttcc cctcttctac
    64141 ccactggtat gcctgttccc ggcttaccac atgaacgcga atatcttcgt tttcatcagc
    64201 cagaccatga ataccgcttg cggtcgtggc gtccacttcg cccaccataa ttgacgaacg
    64261 ctcactggtg ccccccgggc ttgccaggaa acttaacacc ggtttggtcc gtttgactat
    64321 cagtcccgcc tcttcaatcg cttcgcgacg ggcaacatct tccacacttt caccctcttc
    64381 aatcatcccg gcaaccatct ccagtagcca gggggtttcg ctggtgtcgt acgcggcaat
    64441 ccgaatctgc tcaatcagca caacttcatc acgcactggg tcaaagggta gcaagactgc
    64501 ggcgtgaccg cgctcaaaaa tttcccgccg tacctcatga ctcatttgcc cgttgaatag
    64561 acgatgacga aatctataaa gatctaatga aaaaaagccg cgataaagtg tttctcgtgc
    64621 aataatttct acatcgtttt tgccaaatgt aacgggcagg ttgtctggct taagcattgt
    64681 taatgtccta gcactaatag tgaattaaat gtgaatttca gcgacgtttg actgccgttt
    64741 gagcagtcat atgttaaatt gaggcacatt aacgccctat ggcacgtaac gccaaccttt
    64801 tgcggtagcg gcttctgcta gaatccgcaa taattttaca gtttgatcgc gctaaatact
    64861 gcttcaccac aaggaatgca aatgaagaaa ttgctcccca ttcttatcgg cctgagcctt
    64921 tctgggttca gttcgttgag ccaggccgag aacctgatgc aagtttatca gcaagcacgc
    64981 cttagtaacc cggaattgcg taagtctgcc gccgatcgtg atgctgcctt tgaaaaaatt
    65041 aatgaagcgc gcagtccatt actgccacag ctaggtttag gtgcagatta cacctatagc
    65101 aacggctacc gcgacgcgaa cggcatcaac tcgaacgcga ccagtgcgtc cctgcagtta
    65161 actcaatcca tttttgatat gtcgaaatgg cgtgcgttaa cgctgcagga aaaagcagca
    65221 gggattcagg acgtcacata tcagaccgat cagcaaacct tgatcctcaa caccgcgacc
    65281 gcttatttca acgtgttgaa tgctattgac gttctttcct atacacaggc acaaaaagaa
    65341 gcgatctacc gtcaattaga tcaaaccacc caacgtttta acgtgggcct ggtagcgatc
    65401 accgacgtgc agaacgcccg cgcgcagtac gataccgtgc tggcgaacga agtgaccgca
    65461 cgtaataacc ttgataacgc ggtagagcag ctgcgccaga tcaccggtaa ctactatccg
    65521 gaactggcgg cgctgaatgt cgaaaacttt aaaaccgaca aaccacagcc ggttaacgcg
    65581 ctgctgaaag aagccgaaaa acgcaacctg tcgctgttac aggcacgctt gagccaggac
    65641 ctggagcgcg agcaaattcg ccaggcgcag gatggtcact taccgactct ggatttaacg
    65701 gcttctaccg ggatttctga cacctcttat agcggttcga aaacccgtgg tgccgctggt
    65761 acccagtatg acgatagcaa tatgggccag aacaaagttg gcctgagctt ctcgctgccg
    65821 atttatcagg gcggaatggt taactcgcag gtgaaacagg cacagtacaa ctttgttggt
    65881 gccagtgagc aactggaaag cgcacatcgt agcgtcgtgc aaaccgtacg ttcctccttc
    65941 aacaacatta atgcttctat cagtagtatt aacgcctaca aacaagccgt agtttccgcg
    66001 caaagctcat tagacgcgat ggaagcgggc tactcggtcg gtacgcgtac cattgttgat
    66061 gtgttggatg cgaccaccac gctgtacaac gccaagcaag agctggcgaa tgcgcgttat
    66121 aactacctga ttaatcagct gaatattaag tcagccctgg gtacgttgaa cgagcaggat
    66181 ttgctggcac tgaacaatgc gctgagcaaa ccggtttcca ctaatccgga aaacgttgcc
    66241 ccgcaaacgc cggaacagaa tgctattgct gatggttatg cgcctgatag cccggcaccc
    66301 gtcgttcagc aaacatccgc acgcactacc accagtaacg gtcataaccc tttccgtaac
    66361 tgatgacgac gacggggctt cggccccgtc tgaacgtaag gcaacgtaaa gatacgggtt
    66421 atctgccgca ttcttccccc ttctcgcttc aatttcgacc agccatcctc tattctgatg
    66481 ggtatttacc actggtcccg gaagacaaaa atgaaacgga caaaatccat acgccacgca
    66541 tcgttccgca aaaactggag cgcacgccat ctgacaccag tcgctctcgc ggttgccact
    66601 gtttttatgc tggctggctg tgaaaagagt gatgaaacag tgtctctcta tcaaaatgct
    66661 gacgactgtt cagctgcaaa cccaggcaaa agcgccgaat gtaccaccgc gtacaacaat
    66721 gcgctgaaag aagccgaacg tactgcgccg aaatacgcca cccgtgaaga ctgtgttgct
    66781 gaatttggtg aaggtcagtg tcagcaagca ccagcccagg ctggcatggc accagaaaac
    66841 caggcgcagg ctcagcaatc cagcgggagt ttctggatgc cgctgatggc cggttacatg
    66901 atggggcgtc tgatgggcgg cggcgcggga tttgcacagc agccgctgtt ctcctcgaaa
    66961 aacccggcca gtccggctta cggtaaatat accgacgcga cgggtaaaaa ctatggcgca
    67021 gcccagccag gccgcaccat gaccgtaccg aagacggcaa tggcaccaaa accggcgacc
    67081 accactaccg ttacccgtgg cggttttggt gaatctgttg ccaaacaaag cactatgcag
    67141 cgtagcgcaa ccggcacctc ttctcgttca atgggtggct gataccgatg gaaagagtca
    67201 gtattaccga gcgcccggac tggcgtgaga aagcccacga atacggtttc aattttcaca
    67261 ccatgtacgg cgagccgtac tggtgtgaag atgcttacta caagttgacc ctcgcccagg
    67321 ttgaaaagct ggaagaagtc accgccgaac tgcaccagat gtgcctgaaa gtggtggaaa
    67381 aagtgatcgc cagcgatgag ctgatgacca aattccgcat accaaaacac acctggagtt
    67441 ttgtgcgtca atcatggctg actcaccagc catcgcttta ttcgcgtctt gatctggcgt
    67501 gggatggcac tggcgaacct aaacttctgg aaaataacgc cgatacgcca acgtcactat
    67561 acgaggcggc gttctttcag tggatctggc tggaagatca gcttaacgcc ggtaacttgc
    67621 cggagggcag cgaccagttt aacagtctgc aagaaaaact gatcgatcgc ttcgttgagc
    67681 tgcgtgaaca gtatggcttc cagttgctgc atctcacctg ctgtcgcgac acggtggaag
    67741 atcgcggaac cattcagtat ttgcaggact gcgcaaccga agctgaaatc gccaccgagt
    67801 tcctctacat cgacgagata ggtttaggcg aaaaaggcca gttcacggat ttacaggatc
    67861 aggtgatttc taacctgttc aagctttatc cgtgggaatt tatgctgcgc gaaatgttct
    67921 ccaccaagct ggaagatgct ggcgtacgct ggctggaacc ggcgtggaag agcattatct
    67981 ccaacaaggc acttctaccg ctactgtggg agatgttccc gaatcacccg aacctgctgc
    68041 ccgcttattt tgcggaagat gatcatccgc aaatggaaaa atatgtggtt aaaccgatct
    68101 tctcccgtga aggcgcaaac gtgtcgatca ttgagaacgg caaaaccatt gaagcagcgg
    68161 aaggtccgta tggcgaagaa gggatgattg ttcagcaatt ccacccgtta ccgaaattcg
    68221 gcgacagcta tatgctgatt ggtagctggc tggtgaacga tcaacctgcc ggaattggca
    68281 ttcgtgaaga ccgtgcgttg atcacccagg atatgtctcg gttttatcca catatttttg
    68341 ttgaataagc cacgataccg gatggcactc gccatccggt aattgttagc ctatctgcac
    68401 cgacagcata cttaaactgc ccatttctat accatcgacc ggaatggtaa ttggctcctg
    68461 cccatcccac acacctaaca catacaacaa cggcagatag tgctctggcg ttgggttcaa
    68521 taacgcgcca ccttcatggt cgagataatt caccagagga tgctgctcta ccggcccttg
    68581 ccacgtcaga ttcgctttca catactcatt aaacgacgtc gcccacggat acggtgaact
    68641 atcaccgtgc cacttcactg tgcgcaggtt atgcaccacg ttaccgctgg cgaccaacat
    68701 tattccttca tcacgcagcg ccgcgagttt gcgccccatt tcgaaatgcc aggcggcagg
    68761 tttgctactg tcgatactca actgcaccat agggatatcg gcgtcaggat acatcttaat
    68821 cagcacgccc cacgagccgt ggtcaaagcc ccaggcttct ttatccagcg tcaccgggat
    68881 cggcgctaac agctcaacca gacgctgtgc cagcgcaggc gaacccggag caggataatg
    68941 cgtatcgtac agcgcctgcg ggaagccacc aaagtcatga atcgtgggcg gcgtctccat
    69001 cgcggtcact cctgttccac gggtaaacca gtgagccgaa accaccacaa tcgcttgcgg
    69061 gcgtggcaat gtcatcccca acttctgcca gctgcgggta tacaaattat cttccagcac
    69121 gttcatcgga ctaccgtgac ctaaaaacaa tgctggcata cgtgttgaag acatgatgat
    69181 atccttaact aaaggtgtca ttttgatatc ctcacaatac gcttgttcgg cggagtaaga
    69241 acccggataa caatgatgat gatcatcagt tattttgacg atctgcctga aggtgaagat
    69301 ttataaggag ttgtcgatgt cagtacctct cattctgacc atactggcgg gggcagccac
    69361 gtttattggc gcgtttctcg gcgttctcgg acaaaaaccc tcgaaccgct tactggcgtt
    69421 ttcgctaggt tttgcggcgg ggatcatgtt gctcatctca ttaatggaaa tgcttcctgc
    69481 cgcactagca gctgaaggaa tgtcgccggt gttgggttat ggaatgttta tcttcggtct
    69541 gcttggctac tttggcctgg accgcatgtt gccgcatgcg catccgcagg atttaatgca
    69601 aaaatcggtg cagctgttgc caaaatcgat caagcgcaca gccattctgc tcactctcgg
    69661 catcagtctg cataacttcc cggaagggat tgccaccttt gtcacggcga gcagcaacct
    69721 ggagctggga tttggcatcg cactggccgt cgcgttgcac aatatccctg aaggtctggc
    69781 agtggcaggc ccggtttatg cggcaacggg gtctaaacgc accgcgattc tgtgggcggg
    69841 gatttctgga ctagcagaaa ttcttggtgg tgtgctggcg tggttaatcc tcggtagcat
    69901 gatttccccg gtggtcatgg cggcaatcat ggcggcggtt gcaggaatta tggtggcgct
    69961 ctcggttgat gaattaatgc cgctcgccaa agagattgac cctaataata accccagcta
    70021 tggcgtacta tgtggaatgt cagtgatggg attcagttta gtgctgctac aaacggcggg
    70081 aattggttaa aaacaacatc agaagttgtc cagcacatgg acaacttctg ccagcttcac
    70141 gaaagcctta ttctaccttt tcggctgtct cttctctcgt actgtttaag tatttgttcc
    70201 gcgcttcaag cagtgcgtct ctgtcctggc gaaacgcccg acaataacgt ttcaaatgca
    70261 ggaaataacc caccacaacc agagcaatca atacaatcca gtaccaggcg ataaacatcc
    70321 ttttaccctt aatatttaac aatactaaac atttagcgca taaatttcac atatcctttt
    70381 tcggatatat tcatatggtc gcataataac ggaacaatta tatgattttg atcataatga
    70441 aaaagaatgc ctgatattaa tgaaattttc tcatagaaag agaagcggaa ttaacatctt
    70501 gcatagcagg gcaaaataaa aaccgaagcc agctacaact tcggtttctt gattaaggca
    70561 gtaattaagc agcggttttc agctggcttt acgctcatgt gcctgacggt atgccaccag
    70621 gtcttcaata gtcacgagcg ccatattgtg tttattggca aactcaatgc actctggtgc
    70681 acgcgccatc gtgccatcgt cattagtcag ctcacacagc acaccagccg gtttaaagcc
    70741 tgccagcgtc atcaaatcaa tagtcgcttc agtatgaccg ccacgcgtca gtacaccacc
    70801 tgcctgcgcg cgaagtggga aaacgtggcc cgggcgattc agatcagaag gttttgcgcc
    70861 atcggcaatc gctgcgcgaa cggtcgtaat acgatcagcg gcagaaacac cggtagtcac
    70921 accttcagcc gcttcaatgg tcacggtaaa accggtgcca taggcgctgg tgttattttc
    70981 taccatcatt ggcagatcaa gttgtttacg gcgatcttca gtgatgcaca ggcaaacaat
    71041 accgctaccg tggcgaatgg tcagcgccat ctgctcaacg gtcatggttt ctgccgggaa
    71101 gatcatatcg ccttcgtttt cacggtcttc atcatcaagc accattacac cgcgtccttc
    71161 acgcagcgca gccagtgcat tttcaacacg ttcgaaaggc gtaccaaaag aggaaagtag
    71221 cgtctgattc atggtaaaaa aacctcacta aaattatggt taccagaatc agggcagtct
    71281 taggagtggc ggcatatagc caaaataacg tgagcgggtc catgcccgac agaatcgtta
    71341 ctctctccca tccggactct aaccgtcggc cccggaatta caccggatct gctgtccttt
    71401 gagttcgcac ccaaagcgct cgcgggcttt caactgagtt gatttaccgc cggtggggaa
    71461 tttcgccccg ccctgagaat aagcggattc actataacgc taatgattag cggcagcaac
    71521 gcatagcttc acataattct ggtttatgac ttacccttat cgcactacaa tggtactcaa
    71581 cacctatcag tacagggaaa ctacaatgat tgacccgaaa aaaattgagc aaatcgctcg
    71641 ccaggttcac gaatcaatgc ctaaaggaat cagggagttc ggggaagatg tggagaaaaa
    71701 aatccgccaa accctacaag ctcagctgac gcgcctcgac cttgtaagcc gtgaagagtt
    71761 cgacgtccaa acgcaagtcc tgttacggac tcgtgagaaa ctggcgctgc ttgaacaaag
    71821 aatcagcgaa ctggaaaatc gcagtactga gatcaaaaag cagccagatc cagagactct
    71881 ccccccaacg ctgtaaacat ttgtctttaa tgcactgtaa attatgaatt aatcctctaa
    71941 tttacagtgt tttctattac attatcgctt aatcccattc agagtatcat cacaaaaatg
    72001 aatgattgca attgaaaata taattgttat gtattcttta agttttactt aatcgata
//
LOCUS       DAETQL010000006        70995 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.006, whole genome shotgun sequence.
ACCESSION   DAETQL010000006 DAETQL010000000
VERSION     DAETQL010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70995
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.006"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..795
                     /locus_tag="K0W65_000419"
     CDS             <1..795
                     /locus_tag="K0W65_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI3093822.1"
                     /translation="WLPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLD
                     DDNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAF
                     ALIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQ
                     FVVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILY
                     LSGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            806..1897
                     /locus_tag="K0W65_000420"
     CDS             806..1897
                     /locus_tag="K0W65_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3093823.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="K0W65_000421"
     CDS             complement(1940..2800)
                     /gene="rsmI"
                     /locus_tag="K0W65_000421"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBI3093824.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2865..4901
                     /gene="lpoA"
                     /locus_tag="K0W65_000422"
     CDS             2865..4901
                     /gene="lpoA"
                     /locus_tag="K0W65_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HBI3093825.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4859..5254
                     /locus_tag="K0W65_000423"
     CDS             4859..5254
                     /locus_tag="K0W65_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBI3093826.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5274..5864
                     /gene="diaA"
                     /locus_tag="K0W65_000424"
     CDS             5274..5864
                     /gene="diaA"
                     /locus_tag="K0W65_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBI3093827.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5874..6449
                     /gene="yraP"
                     /locus_tag="K0W65_000425"
     CDS             5874..6449
                     /gene="yraP"
                     /locus_tag="K0W65_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBI3093828.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6563..7603)
                     /locus_tag="K0W65_000426"
     CDS             complement(6563..7603)
                     /locus_tag="K0W65_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBI3093829.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7676..8311)
                     /locus_tag="K0W65_000427"
     CDS             complement(7676..8311)
                     /locus_tag="K0W65_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBI3093830.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8439..8957
                     /gene="yhbO"
                     /locus_tag="K0W65_000428"
     CDS             8439..8957
                     /gene="yhbO"
                     /locus_tag="K0W65_000428"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBI3093831.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8937..9380)
                     /locus_tag="K0W65_000429"
     CDS             complement(8937..9380)
                     /locus_tag="K0W65_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBI3093832.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9431..9733
                     /gene="yhbQ"
                     /locus_tag="K0W65_000430"
     CDS             9431..9733
                     /gene="yhbQ"
                     /locus_tag="K0W65_000430"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBI3093833.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9720..10223)
                     /locus_tag="K0W65_000431"
     CDS             complement(9720..10223)
                     /locus_tag="K0W65_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBI3093834.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10217..10741)
                     /locus_tag="K0W65_000432"
     CDS             complement(10217..10741)
                     /locus_tag="K0W65_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBI3093835.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10950..11945
                     /locus_tag="K0W65_000433"
     CDS             10950..11945
                     /locus_tag="K0W65_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBI3093836.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11954..12832
                     /locus_tag="K0W65_000434"
     CDS             11954..12832
                     /locus_tag="K0W65_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBI3093837.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12913..13920
                     /locus_tag="K0W65_000435"
     CDS             12913..13920
                     /locus_tag="K0W65_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBI3093838.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="K0W65_000436"
     CDS             complement(14038..15282)
                     /gene="mtr"
                     /locus_tag="K0W65_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBI3093839.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="K0W65_000437"
     CDS             complement(15436..17325)
                     /gene="deaD"
                     /locus_tag="K0W65_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HBI3093840.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="K0W65_000438"
     CDS             complement(17318..17398)
                     /gene="yrbN"
                     /locus_tag="K0W65_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBI3093841.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="K0W65_000439"
     CDS             complement(17505..18389)
                     /gene="nlpI"
                     /locus_tag="K0W65_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBI3093842.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="K0W65_000440"
     CDS             complement(18498..20633)
                     /gene="pnp"
                     /locus_tag="K0W65_000440"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBI3093843.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="K0W65_000441"
     CDS             complement(20880..21149)
                     /gene="rpsO"
                     /locus_tag="K0W65_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBI3093844.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21298..22242)
                     /gene="truB"
                     /locus_tag="K0W65_000442"
     CDS             complement(21298..22242)
                     /gene="truB"
                     /locus_tag="K0W65_000442"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBI3093845.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="K0W65_000443"
     CDS             complement(22242..22643)
                     /gene="rbfA"
                     /locus_tag="K0W65_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBI3093846.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22807..25455)
                     /gene="infB"
                     /locus_tag="K0W65_000444"
     CDS             complement(22807..25455)
                     /gene="infB"
                     /locus_tag="K0W65_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBI3093847.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="K0W65_000445"
     CDS             complement(25480..26967)
                     /gene="nusA"
                     /locus_tag="K0W65_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBI3093848.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="K0W65_000446"
     CDS             complement(26995..27447)
                     /gene="rimP"
                     /locus_tag="K0W65_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBI3093849.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27654..27730)
                     /locus_tag="K0W65_000447"
     tRNA            complement(27654..27730)
                     /locus_tag="K0W65_000447"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27694..27696),aa:Met,seq:cat)
     gene            28078..29421
                     /gene="argG"
                     /locus_tag="K0W65_000448"
     CDS             28078..29421
                     /gene="argG"
                     /locus_tag="K0W65_000448"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBI3093850.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29429..31036)
                     /locus_tag="K0W65_000449"
                     /pseudo
     CDS             complement(29429..31036)
                     /locus_tag="K0W65_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31514..31600)
                     /locus_tag="K0W65_000450"
     tRNA            complement(31514..31600)
                     /locus_tag="K0W65_000450"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31564..31566),aa:Leu,seq:gag)
     gene            complement(31615..31947)
                     /gene="secG"
                     /locus_tag="K0W65_000451"
     CDS             complement(31615..31947)
                     /gene="secG"
                     /locus_tag="K0W65_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBI3093851.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="K0W65_000452"
     CDS             complement(32175..33512)
                     /gene="glmM"
                     /locus_tag="K0W65_000452"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBI3093852.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33505..34353)
                     /gene="folP"
                     /locus_tag="K0W65_000453"
     CDS             complement(33505..34353)
                     /gene="folP"
                     /locus_tag="K0W65_000453"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBI3093853.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="K0W65_000454"
     CDS             complement(34443..36377)
                     /gene="ftsH"
                     /locus_tag="K0W65_000454"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBI3093854.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="K0W65_000455"
     CDS             complement(36477..37106)
                     /gene="rlmE"
                     /locus_tag="K0W65_000455"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBI3093855.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37232..37525
                     /gene="yhbY"
                     /locus_tag="K0W65_000456"
     CDS             37232..37525
                     /gene="yhbY"
                     /locus_tag="K0W65_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBI3093856.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37681..38157)
                     /gene="greA"
                     /locus_tag="K0W65_000457"
     CDS             complement(37681..38157)
                     /gene="greA"
                     /locus_tag="K0W65_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBI3093857.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38405..39838
                     /gene="dacB"
                     /locus_tag="K0W65_000458"
     CDS             38405..39838
                     /gene="dacB"
                     /locus_tag="K0W65_000458"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBI3093858.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="K0W65_000459"
     CDS             complement(40024..41196)
                     /gene="cgtA"
                     /locus_tag="K0W65_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBI3093859.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41212..42177)
                     /locus_tag="K0W65_000460"
     CDS             complement(41212..42177)
                     /locus_tag="K0W65_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI3093860.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="K0W65_000461"
     CDS             complement(42304..42561)
                     /gene="rpmA"
                     /locus_tag="K0W65_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBI3093861.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="K0W65_000462"
     CDS             complement(42582..42893)
                     /gene="rplU"
                     /locus_tag="K0W65_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBI3093862.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43151..44122
                     /gene="ispB"
                     /locus_tag="K0W65_000463"
     CDS             43151..44122
                     /gene="ispB"
                     /locus_tag="K0W65_000463"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBI3093863.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44349..44627
                     /gene="sfsB"
                     /locus_tag="K0W65_000464"
     CDS             44349..44627
                     /gene="sfsB"
                     /locus_tag="K0W65_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBI3093864.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44675..45934)
                     /gene="murA"
                     /locus_tag="K0W65_000465"
     CDS             complement(44675..45934)
                     /gene="murA"
                     /locus_tag="K0W65_000465"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBI3093865.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="K0W65_000466"
     CDS             complement(45989..46243)
                     /gene="ibaG"
                     /locus_tag="K0W65_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBI3093866.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="K0W65_000467"
     CDS             complement(46403..46696)
                     /gene="mlaB"
                     /locus_tag="K0W65_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBI3093867.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="K0W65_000468"
     CDS             complement(46696..47331)
                     /gene="mlaC"
                     /locus_tag="K0W65_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBI3093868.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="K0W65_000469"
     CDS             complement(47350..47901)
                     /gene="mlaD"
                     /locus_tag="K0W65_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBI3093869.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="K0W65_000470"
     CDS             complement(47906..48688)
                     /gene="mlaE"
                     /locus_tag="K0W65_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBI3093870.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="K0W65_000471"
     CDS             complement(48696..49505)
                     /gene="mlaF"
                     /locus_tag="K0W65_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBI3093871.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49715..50680
                     /locus_tag="K0W65_000472"
     CDS             49715..50680
                     /locus_tag="K0W65_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBI3093872.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50694..51680
                     /gene="kdsD"
                     /locus_tag="K0W65_000473"
     CDS             50694..51680
                     /gene="kdsD"
                     /locus_tag="K0W65_000473"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBI3093873.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51701..52267
                     /gene="kdsC"
                     /locus_tag="K0W65_000474"
     CDS             51701..52267
                     /gene="kdsC"
                     /locus_tag="K0W65_000474"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBI3093874.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52264..52839
                     /gene="lptC"
                     /locus_tag="K0W65_000475"
     CDS             52264..52839
                     /gene="lptC"
                     /locus_tag="K0W65_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBI3093875.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52808..53365
                     /gene="lptA"
                     /locus_tag="K0W65_000476"
     CDS             52808..53365
                     /gene="lptA"
                     /locus_tag="K0W65_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBI3093876.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53372..54097
                     /gene="lptB"
                     /locus_tag="K0W65_000477"
     CDS             53372..54097
                     /gene="lptB"
                     /locus_tag="K0W65_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBI3093877.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54145..55578
                     /gene="rpoN"
                     /locus_tag="K0W65_000478"
     CDS             54145..55578
                     /gene="rpoN"
                     /locus_tag="K0W65_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBI3093878.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55601..55888
                     /gene="hpf"
                     /locus_tag="K0W65_000479"
     CDS             55601..55888
                     /gene="hpf"
                     /locus_tag="K0W65_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBI3093879.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56006..56497
                     /gene="ptsN"
                     /locus_tag="K0W65_000480"
     CDS             56006..56497
                     /gene="ptsN"
                     /locus_tag="K0W65_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBI3093880.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56543..57397
                     /gene="rapZ"
                     /locus_tag="K0W65_000481"
     CDS             56543..57397
                     /gene="rapZ"
                     /locus_tag="K0W65_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBI3093881.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57394..57666
                     /gene="npr"
                     /locus_tag="K0W65_000482"
     CDS             57394..57666
                     /gene="npr"
                     /locus_tag="K0W65_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBI3093882.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57879..58511
                     /gene="yrbL"
                     /locus_tag="K0W65_000483"
     CDS             57879..58511
                     /gene="yrbL"
                     /locus_tag="K0W65_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBI3093883.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="K0W65_000484"
                     /pseudo
     CDS             complement(58508..59235)
                     /gene="mtgA"
                     /locus_tag="K0W65_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="K0W65_000485"
     CDS             complement(59232..59885)
                     /gene="elbB"
                     /locus_tag="K0W65_000485"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBI3093884.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="K0W65_000486"
     CDS             complement(60115..62451)
                     /gene="arcB"
                     /locus_tag="K0W65_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBI3093885.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62547..63476)
                     /locus_tag="K0W65_000487"
     CDS             complement(62547..63476)
                     /locus_tag="K0W65_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBI3093886.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64151..68611
                     /gene="gltB"
                     /locus_tag="K0W65_000488"
     CDS             64151..68611
                     /gene="gltB"
                     /locus_tag="K0W65_000488"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBI3093887.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68624..70042
                     /gene="gltD"
                     /locus_tag="K0W65_000489"
     CDS             68624..70042
                     /gene="gltD"
                     /locus_tag="K0W65_000489"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HBI3093888.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70226..70705
                     /locus_tag="K0W65_000490"
     CDS             70226..70705
                     /locus_tag="K0W65_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBI3093889.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            70780..70995
                     /locus_tag="K0W65_000491"
                     /pseudo
     CDS             70780..70995
                     /locus_tag="K0W65_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT        17403 a        19234 c        17627 g        16731 t
ORIGIN      
        1 tggttaccgc gtagcctggt ttcctatcag atgaccagcc agaaagatcg cccaacccaa
       61 catgaaatgc gtcttgatgg ctcactgctg gatgatggtc gcctgagcta tagcctggaa
      121 caaagtctgg atgacgataa caaccataac agtagcctga acgccagtta ccgttcacct
      181 tatggaacct tcagtgccgg atacagttac ggtaatgaca gcagccaata caattacggc
      241 gttaccggcg gcgtggttat ccatcctcat ggcgtgacgc tctcgcaata tctgggcaac
      301 gcttttgcgc ttatcgatgc taacggggct tctggcgtga ggatacaaaa ctatccgggg
      361 attgctactg atccctttgg ctatgcagtg gttccttatc tcacgactta tcaggaaaac
      421 cgtctctcgg tagatactac gcagctgccc gataacgtcg atcttgaaca aacaacacag
      481 tttgtggtgc ccaacagagg tgcaatggta gcggcgcgtt tcaacgccaa tatcggttat
      541 cgcgtacttg ttacagtcag cgatcgcaac ggtaaaccgt tgccctttgg cgctcttgcc
      601 agcaacgatg atacggggca acaaagtatc gtcgatgagg gcggcatact atatctctct
      661 gggatatcga gtaaatcaca aagctggact gtacgctggg gaaatcaggc agatcaacaa
      721 tgtcagtttg cttttagtac accggattca gaaccaacaa cctctgtatt acaaggcaca
      781 gcgcagtgcc attaaggata aaaaaatgaa aagagcgcct cttataacag gacttttgtt
      841 gatatccaca tcctgcgctt atgcctcctc aggagggtgt ggagccgaca gcactagcgg
      901 tgcgacaaat tacagcagtg tggttgatga tgttacggtg aaccagacag ataacgtgac
      961 aggacgggag tttacctctg caacgctaag tagcactaac tggcaatacg cctgttcctg
     1021 ctctgcgggt aaggcagtta aacttgtcta tatggtcagc cccgtactta ccaccactgg
     1081 acatcagaca ggatattaca aactcaatga cagcctggat attaaaacca cattacaggc
     1141 aaacgacatt ccaggactca caaccgacca ggttgtctct gttaacaccc gattcacaca
     1201 gataaaaagc agcaccgtat attctgctgc aacccaaacg ggtgtttgcc agggtgacac
     1261 gtctcgttat ggacccgtta atattggtgc aaataccacc tttaccctgt atgtcaccaa
     1321 gccatttctc ggctcgatga ccattccgaa aacggatatt gccgtcatta aaggcgcgtg
     1381 ggtcgatgga atgggaagcc cgtctacagg tgacttccat gatttagtca agttatcgat
     1441 tcagggaaat ctcaccgccc cacagtcgtg caaaattaat cagggcgatg ttattaaagt
     1501 taattttgga ttcatcaatg gtcagaagtt taccacccgc aatgccatgc cagacggttt
     1561 tactccagta gactttgata tcacttatga ctgtggtgat acttcaaaga ttaaaaactc
     1621 gttgcaaatg cgcatcgacg gtacaactgg ggtagtagac cagtacaacc tggtcgccag
     1681 gcgaagaagt tcagacaatg tgcccgatgt cggtattcgt attgaaaatc tcggcggcgg
     1741 agttgcaaat attccttttc agaacggtat ccttcccgtt gatccttccg ggcatggcac
     1801 cgtcaatatg cgcgcctggc cagttaatct ggtcggtggt gagctggaaa caggaaaatt
     1861 tcagggcaca gccaccatta ccgtcatggt gcggtaaaag aagacgttgc gttttgatta
     1921 acgcaacgct tcggcactgt tacccctgct gctccagcgc atacttatac agcgcatttt
     1981 tcttcacgcc atgaatttct gcggccagcg ccgccgcttt tttcagcggc agttctgcct
     2041 gtagcagcgc cagcgtgcgc agggcatcgg cgggtaagtc ttcttcctgt gctttatgac
     2101 cttcgacaat cagcaccatt tcgcctttgc gacggttttc atcttccttt acccacgcca
     2161 gcagctcgcc aacgggcgcg ccgtgaatgg tttcccaggt tttggtcagc tcacgcgcca
     2221 gaaccacgta gcgggattcg cctaataccg caacgatatc ttccaggcta tctaacagac
     2281 ggtgggtaga ttcataaaaa atcagcgtgc gcggctccgc ttcaatggct tttagcgcat
     2341 cacggcggcc ttttgatttg gcaggtaaaa agccttcgta acagaaacgg tcagatggta
     2401 aacccgcggc gcttaacgca gtgatagcag cacacggtcc gggtagcggc accacacgaa
     2461 tccccgcttc acggcaggta cgcaccagat ggtagccagg atcgttaatt agcggcgttc
     2521 cggcatcgga aaccagcgca atgttttgcc cctcttgcag ctttgccagc agcgtttcgg
     2581 ctttttgttg ttcgttatgg tcgtgcagcg caaacaaccg ggcattaatc ccaaaatgtt
     2641 gcagcaataa accggtgtga cgagtatcct cggcggcaat cagatcaacg gcctgtaata
     2701 cctctaacgt acgctgggtg atatccgcca gattgccgat tggcgtcggt acaatgtaaa
     2761 gctggccctg agaattatcc gccgattggt gttgtttcat tgtgtcgtcc gtattgccga
     2821 tttaatattg agcattgcgt aaaaaaaata tcactggata cattatggta ccctcaacat
     2881 tttctcgttt gaaagccgcg cgttgtctgc ctgttgttct ggcagccctg attttcgccg
     2941 gttgtggcac ccatactccc gatcagtcca ctgcttatat gcagggcacg gcgcaggctg
     3001 attctgcctt ttatcttcag cagatgcagc aaagctctga tgataccagg atcaactggc
     3061 aattactcgc cattcgtgca ctggtgaaag aaggtaaaac cgggcaggcg gttgagttgt
     3121 ttaaccaact accgcaagaa ctgaacgatt ctcagcgtcg cgagaaaaca ctgctggcgg
     3181 cagagattaa actggcgcag aaagattttg ctggcgcgca aaacttgctg gcgaaaatca
     3241 cacctgccga tttagaacaa aaccagcaag cgcgttactg gcaggcaaaa atcgatgcca
     3301 gccaggggcg tccttccatt gatttactgc gcgcgttaat tgctcaggaa ccgctgcttg
     3361 gcgcgaaaga aaaacagcag aatattgatg ccacctggca ggcgctctcc tccatgactc
     3421 aggaacaggc gaatacgctg gtgatcaacg ccgacgaaaa tattctgcaa ggctggctgg
     3481 atctgcagcg cgtctggttt gataaccgta acgatcccga catgatgaaa gccgggatcg
     3541 ccgactggca gaaacgttat ccgaacaatc cgggcgcgaa aatgctgcca acgcagttgg
     3601 ttaacgtaaa agcgtttaaa ccagcctcga ccaacaaaat cgccctgctg ttgccgctga
     3661 atggccaggc agcggtattt ggtcgcacta ttcagcaagg ctttgaagcc gcgaaaaata
     3721 tcggcactca gccagtggcg gctcaggtag ctgccgcacc tgccgcagac gtagcagaac
     3781 aacctcagcc gcaaactgcg gatagcgttg ccagcccggc acaagcctcg gttagcgatc
     3841 tgaccggtga tcagcctgcg gcccagccgg tgcctgtaag cgccccggcg acaagcaccg
     3901 cagcggtaag cgcacccgca aatccatccg cagagctgaa aatctacgat acctcatcac
     3961 aaccacttag ccagatctta agccaggttc agcaggatgg cgcgagtatt gtggttggtc
     4021 cgttgctgaa aaataacgtt gaagagttgc tgaagagcaa caccccgctg aacgtactgg
     4081 cactgaacca gccggagaat atcgaaaacc gcgtcaatat ttgttacttc gcgctttcac
     4141 cggaagacga agcgcgcgat gcagcgcgtc atattcgtga ccagggtaaa caagcgccgc
     4201 tggtgctgat cccacgcagt gcattgggcg atcgcgtagc caatgcgttt gcgcaagagt
     4261 ggcagaaact gggcggcggc accgttctgc aacaaaaatt tggttccacc agcgaattac
     4321 gcgcgggtgt taacggcggt tctggtattg ctttaacggg tagcccgatt actcccagag
     4381 cgacaaccga ctccggcatg acgaccaaca atccaacgct gcaaaccacg ccaaccgatg
     4441 accagttcac caataatggc ggtcgtgtcg atgcggtcta cattgtggca acgccgggtg
     4501 aaatcgcttt tatcaaaccg atgatcgcca tgcgtaacgg tagccagagc ggtgcaacgc
     4561 tgtacgccag ttcccgcagc gcacaaggca ccgcaggccc ggatttccga ctggagatgg
     4621 aaggcttgca gtacagcgaa atcccgatgc tggcaggcgg taatctgccg ttaatgcagc
     4681 aggcgctcag cgcggtgaac aacgattatt cgctggctcg catgtatgcg atgggcgttg
     4741 atgcctggtc gttggcaaat catttctcac aaatgcgcca ggttcagggt tttgaaatca
     4801 acggtaatac cggaagtctg acggctaacc cggattgcgt gattaacagg aagttatcat
     4861 ggctacagta ccaacaaggt caggtagtcc ccgccagtta accaccaaac agaccggcga
     4921 tgcgtgggaa gcacaagcgc gtcgctggct ggaaggcaaa ggactgcggt ttatcgccgc
     4981 taacgtgaac gagcgtggcg gcgagatcga tctgataatg cgtgaaggcc tgaccaccgt
     5041 ttttgtcgag gtacgctatc gccgctctgc gctttatggc ggcgcggcag ccagtgtgac
     5101 ccgcagcaaa caacacaaat tattacagac tgcccgcttg tggctcgcgc gtcataatgg
     5161 gagttttgat actgtggatt gccggttcga tgtggtagcc ttcaccggga atgaggttga
     5221 gtggattaag gatgccttta atgaccactc ataattaagg tttaaggatt agcgtgcaag
     5281 aaagaattaa agcttgcttc actgaaagca ttcaaactca aattgcggcg gcagaggcgc
     5341 ttccggatgc catctcccgt gcagccatga cgctggttca gtctctgctc aatggcaaca
     5401 aaatcctctg ttgtggtaat ggaacttccg ctgccaatgc acagcatttt gctgccagca
     5461 tgatcaaccg tttcgaaacg gagcggccca gcttacctgc cattgcacta aatactgata
     5521 atgttgtctt aacggcgatt gccaacgatc gcttacatga tgaagtgtat gcaaaacagg
     5581 tgcgggcgct gggtcatgcg ggagatgtat tgttagccat ttccacccgt ggcaacagcc
     5641 gcgatattgt taaagcagtt gaagccgccg ttacgcgtga tatgaccatt gtggcattga
     5701 ccggctatga cggcggcgaa cttgcaggtt tgttagggcc acaggatgtg gagatccgca
     5761 ttccttcgca tcgtagtgct cgcattcagg aaatgcatat gctgacggta aattgcctgt
     5821 gcgatctgat cgataacacg cttttccctc accaggatga ttaaggagaa tacatgaagg
     5881 cattatcgcc aatcgcagtc cttatttccg cgctgctgtt gcaaggttgt gttgccgctg
     5941 ccgtagtggg taccgctgct gtgggtacca aagccgcaac tgacccacgc agtgtcggca
     6001 cccaggtgga cgatggtacc ctggaagtgc gcgtgaacag cgcattgtcg aaagacgaac
     6061 agattaagaa agaaacccgt attaatgtca cggcctatca gggcaaagtg ctgctggttg
     6121 ggcagtcacc aaatgctgaa ctttcggctc gcgccaaaca gattgctatg ggcgtagacg
     6181 gtgccaacga agtgtataac gagattcgtc agggccagcc gattggtctg ggcgaagcat
     6241 ctaacgatac gtggatcacc accaaagtgc gttcgcagct cttaaccagc gacctggtga
     6301 aatcgtccaa cgtgaaagtg accaccgaaa acggtgaagt gttcctgatg gggctggtga
     6361 ctgaacgtga agcgaaagcg gcggcagata ttgccagccg ggtgagcggc gtaaagcggg
     6421 ttaccacagc gtttacgttt attaaatagc agccccttgt aatgcctgat gcgacgcttg
     6481 ccgcgtctta tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc
     6541 atccgacatg ttttccctca aatcagaaca acagcgccag cccgccgaca atcacaccag
     6601 acactgccac catcgcccct gtcagccata aggctttcgc cgggaacgct ttgcgcagca
     6661 taatcagtga cggcaaactc actgccggga gcgtcatcaa caatgccagc gccggagcgg
     6721 ttcccatacc tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga
     6781 caaacaagca tcctgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac
     6841 catcggcatg ggggaataac cagacgcgtg cggcacccaa caccagtact gcaaggatgt
     6901 aaaccgggat cgtactccag aaaagtgtcc atagcgccct gccccagcgg ctaaaaaaac
     6961 cgctctgtgc ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca
     7021 cccatttttg caccagcgtc gcaatcagca acaccatcac cagcccggcc accagacgaa
     7081 tcgccgcaaa accccagccg aggacaaacc ccataaacac cagcgtcgcc gggtttaaca
     7141 ccggattgcc catccagaat gccagcgcac cgcccatcga tacctgttgg cgacgcattc
     7201 ccgccgcgac cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc
     7261 ccagcagcgt gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga
     7321 tcagcacctg aatcaacgaa ccgaggatca cccccagcac cgccgctttc catactgcga
     7381 ggaaatagat catcgcgtaa tccaacgccg cttgccacgg attagcatcc gcctgcgcaa
     7441 ggatagattt accgatactg tgggtttcgg ctgcagtaaa cgctttgccg tagtaaggtt
     7501 gccatttcac ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc
     7561 actgaatggg cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata
     7621 agttatgata attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt
     7681 cagctctttt gcgtaattct gaagagctta aaatcgtcac gccttcatgc tcaggccgca
     7741 tcgcctctgc cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttacctg
     7801 gtaacaaacg gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc
     7861 ccagtaacat cgacgggcga gcaatggtca atttcggcca gttctgggca attaatgctt
     7921 cttccatctc ccctttgacg cggttataga aaaacggcga gtgggcattg gcacccatag
     7981 cactgaccac cagcatatgc tgcgcgccca gtcgccgccc ggttaatgcg gtatccacta
     8041 ccagcgtgta atcggcatga ataaacgcct ctttgctccc cgcttctcgc cgcgtggtgc
     8101 cgagacaaca aaacacaatg tcgatgggtt cggtgacctg cgccaacgcg tcactcagtt
     8161 gcggatcatg gggattaaac accccgggca tatcgcccaa cggacgtcgc gtcggcgcag
     8221 caatggcgtt aactttcggt tcattaatca acatccgcag caggtgaccg cccaccagcc
     8281 ccgttgcgcc tgtaatcagt acctgactca tcttcgttcc tttacagaat tgtctgcctt
     8341 gcgctccccg gctcatgcac cagtcttaat agaccatgag gtaattatcc ccgattgtgg
     8401 aaaattcgcc tcatccaatg caacaacgcg gaggaagcat gagtaagaaa attgccgttt
     8461 taatcactga tgaatttgaa gattcagaat ttacttcacc cgcagacgag ttccgtaaag
     8521 ccggacacga agtgattact attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag
     8581 gagaagccag cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg
     8641 ccctgttgct accgggtggt cattcaccgg attatctgcg tggcgacaac cgttttgtca
     8701 cctttacccg tgattttgtg aatagcggca aacctgtttt cgccatctgt cacggtccgc
     8761 agctgctgat cagcgccgat gtgattcggg ggcgcaaact gaccgcagtt aaaccgatca
     8821 ttattgatgt taaaaatgcg ggcgtggaat tttacgatca ggaagtcgtg gttgataaag
     8881 atcagctggt taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac
     8941 gcctgctcgg tgcctgagtc gcgtagccaa atcatttttt taccaaagcc cagcgtgttg
     9001 tcggtgaatt tgatttcatc gagccggatt tcccacaccg gggccgacag cattctggca
     9061 accggaaagc gacgattgta cgccttgcgc gcgaggtcgc tttcctcacc ttccagcctg
     9121 cggatctcac ctttaaactg cacaccgcga attaacgcta ccgttttcgg ctgaccgttt
     9181 accgttccgg caaccgcagc ctgcggccca ctcatctgcg cgtggcgcgt tttttcttcc
     9241 gtcaaaatgt agaaggcgac tttctgcgca tcaaaaagat aaaaggcatt agcgcaccag
     9301 agttcccctt cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg
     9361 atggcgatga gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc
     9421 gatgcagacg atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta
     9481 taccgggatc accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc
     9541 gaaagcactg cgcgggaaag gagaactgac gctggcgttt tctgcgccag tgggcgaccg
     9601 ttcgctggca ctacgggcgg aatatcgcgt taagcaactg accaaacggc aaaaagagcg
     9661 tctggtagcg gaaggcgcag ggtttgcaga actgttgagc agcctgcaaa ccccggagat
     9721 taaaagcgat tgaaatgctc gtgatactca accagaccgg ttacgccatt cagcgcgtca
     9781 tccgctaaac gatgtaccag gaaggcgctt tcagtgcccg gccagcggca acgcagatcg
     9841 tgatgcgccg ccagttcaaa gccgaaacga ctgtacagcg ccggatcgcc cagcgtcacc
     9901 actgcggcat agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc
     9961 agcccctgtc cacggtattt ttcatcaacc gccaacggtg ccatgccgac ccattgcagg
    10021 tcttcgccct gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca
    10081 tctgtcgcca ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc
    10141 gcttccgcat cactttcgaa tgagcgacgc agcagggcat caatacccgg cgcatcaatg
    10201 ggaatttcta ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg
    10261 ttttcattcc cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg
    10321 gcatttgctc cagttcaatg gcgtccatca ggtttttcac atacaccccc agctccgtat
    10381 cgccttcaat caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg
    10441 caatcatcag cagatcgctg gcgtcggcac taaaactcac atcagcttgc gtgttctggc
    10501 tgaccaccag tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat
    10561 gaatacttaa ccagcggcct tcaagaaact ccagctcgcc atcatccagc gcctgacgga
    10621 attgccagct taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta
    10681 ccggtacact caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca
    10741 cgagttttac tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag
    10801 cggtataaat caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc
    10861 ttaaatcaaa aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg
    10921 ttttgatagc gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc
    10981 cggcgcttaa ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg
    11041 ataccaatgc ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg
    11101 tgagttttgt ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc
    11161 cggacggtta cgcccgttgg cagcgtgccg tggatatggc ggcgcagctg ggtgccgacg
    11221 cgctgatcct cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg
    11281 aacgtcatgt gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc
    11341 gccattttga cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac
    11401 agctggcacg ggttactcct gtcccgctgg aagtgtttgc tttcggcagc ctgtgtatta
    11461 tgtcggaagg ccgttgctat ctgtcgtcgt atctgacggg tgagtcgccc aacactgtgg
    11521 gtgcgtgttc tccggcccgt ttcgtgcgct ggcaacaaac gccgcagggg ctggaatccc
    11581 gcctgaacga agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc
    11641 tgtgtaaagg gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaaccaacca
    11701 gtctcaatac cctggaactg ctgccggagt taatggctgc gaatattgct tcggtgaaaa
    11761 ttgaaggccg ccagcgtagc ccggcgtatg tcagccaggc ggcgaaagtc tggcgtcagg
    11821 ctatcgaccg ttgtaaggcc gatccgcaaa acttcgtacc gcaaagcgcg tggatggaga
    11881 cgctcgggtc gatgtccgaa ggcacgcaaa ccacccttgg cgcgtatcac cgtaaatggc
    11941 agtgagaaga gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac
    12001 gctggaagat ttttatcagc aagccgccgc cagcagcgcc gacgtgattt atcttggtga
    12061 agcggtatgc agcaagcgcc gggcaaccaa agttggcgac tgtctggaga tggcaaaatc
    12121 gctcgccgga agtggcaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc
    12181 tgaactgggc gaactgaaac gctatgttga gaacggtgaa tttctgattg aagccagcga
    12241 tctcggcgtg gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg ggcacgcgct
    12301 gaactgctac aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg
    12361 tatgccggtg gagctttccc gcgactggct ggtgaatctg cttaatcagt gcgatgagct
    12421 gggcattcgc aaccagtttg aagtggaagt cctgagctac ggtcatctac cgctggccta
    12481 ctccgcccgc tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg
    12541 ctgcattaag tatccgaacg ggcgcaacgt gctgtcgcag gaaaaccaac aagtgtttgt
    12601 actcaatggc attcagacca tgagcggcta cgtttacaac ctcggtaacg agctggcatc
    12661 catgcaggga ttggttgatg tagtccgcct gtcaccgcag ggcactgaca ctttcgcgat
    12721 gctcgacgcc ttccgcgcta atgaaaatgg cgctgtgcca ctgccgttga cggcaaacag
    12781 cgactgtaac ggctactggc ggcggctggc aggactggag ctgcaagcct aagtaaatag
    12841 ctcactttgt taacaacttt aactattctt taatgcagta ttaaagatta atcggtaaca
    12901 aagtgagctg ttatgactga taaaaccatt gcgttttcgc tactcgatct ggcccccata
    12961 cccgaaggct cttcagcgcg ggaagcgttc tcccactctc tcgatctcgc ccgtctggct
    13021 gaaaagcgcg gctatcatcg ctactggctg gcagaacacc acaatatgac cggcattgcc
    13081 agtgctgcaa catcggtatt gattggctat ctggcggcga ataccaccac gctgcatctg
    13141 gggtctggcg gcgtgatgtt gcctaaccac ttaccgttgg tcattgccga acagttcggc
    13201 acactcaata cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt
    13261 gaccaacgga caatgatggc gctacgtcgt catatgagcg gcgatattga taatttcccc
    13321 cgcgatgtcg cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc
    13381 ccggtaccag gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagt
    13441 gcacaactgg cggcgcagct tggtctgcca tttgcgtttg cctcacactt cgcgccggat
    13501 atgttgttcc aggcgctgca tctttatcgc agtaacttca aaccgtcggc tcggctggaa
    13561 aaaccatacg cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa
    13621 tttctgttta cctcaatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa
    13681 ctgccgccgc cgattcaaaa tatggatcag ttctggtcac cgtctgagca gtatgacgtg
    13741 cagcaggcgc tgagtatgtc gttggtaggc gataaagcga aagtgcgtca tggtttgcag
    13801 tcgatcctgc gcgaaaccga cgctgatgag attatggtta acgggcagat tttcgaccac
    13861 caggcgcgcc tgcattcgtt tgagctggcg atggatgtta aggaagagtt gttgggatag
    13921 tgtgtcttaa cgcgggaagc cttatccgag ctggcaacgc tgtcctacgt aggcctgata
    13981 agcgaagcgc atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta
    14041 ctgatacacc ggcagtaaat taaagctcga taaaatatgc accagtgcgt tgccgacgcc
    14101 aaacaccaga atcagcgcaa tcatcggctt gccaccccag acgcggaatt tcgggctgcc
    14161 aaagcgttta cgcgatgcgc gggccagcag tgccggaaca attgccgccc agatggtagc
    14221 cgctaaacca gcataaccaa tggcatacag gaatccgttc gggaacagca gaccacccac
    14281 aacaggcggg gcaaaggtca gcaatgccgt tttcaagcgg cccacagccg agtcgtcgaa
    14341 accaaacaga tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc
    14401 taccgcaaag tttgagaaca cgaccagcag aaggtccagg ctacggctgt tcagcacgcc
    14461 gcttaatgct tgtaccaaca catcaatatt accgcccttc tcggcgatgc cgataaactc
    14521 cggacgaggg atgttgccca ttgtcgccag caaccagatg gtatacagcg ccagtgccat
    14581 cagcgtaccg tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat
    14641 caggcttggc acgttaccgt gataaccaaa cgacgccagg cagaacggca gggtcatcag
    14701 caaatacggc gcataagatg cattgctttc ggcaacgttg aacaatgtcg caggctgcac
    14761 atgccccagc aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat
    14821 cgccgtcatg cggctgacgg ctttagtact caaccacacc acaaacgcta ccagcagtgc
    14881 aaaaccaaaa cccgccgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg
    14941 cagaatcgaa ccactggcag aaatataggc ataggtcagg atatagagca caaaggcaat
    15001 ggaaatgccg ttgaccacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa
    15061 actcgaaccg attcggtaat tcaggttagc ttccagaatc atcaagccgg aatgcagcat
    15121 acagaaccag gtaaagatca gcgctgccat tgaccagaaa aaccacgccc cggacatgac
    15181 cactggcaga gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc
    15241 aagcagcgac ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg
    15301 aaaaatagtg cgactgcgtt gttatgcatt gcactgtacc agtacatgag tacaaaagac
    15361 agaaaaaaag ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc
    15421 gctgttcagc gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg
    15481 acgcggtgcg cggccttcac gacgttcgcc gctaaaacga cgaccatcac cacgaccacc
    15541 ttcacggcgt tcaccgctga agttacgacc gccttcacga cgctcgccac cgaaaccacg
    15601 accaccgcca cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt
    15661 catcggcttg ttgagaatgc gagtgcgcgt aaagtgttgc agcacttcac ccggcatacc
    15721 tttcggcagt tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct
    15781 gctgatgtcg ccttcgttag caatcgcgcc aacgatatga cgaacttcaa caccatcatc
    15841 gcggcccact tcaatgcggt acagctgcat atcgccaaca tcacgacgtt cacgacgcgg
    15901 acgatcttca cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc
    15961 acggtcacgg aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg
    16021 ttcaccctgt gccattttca gcagtgccgc agccagagtt tcgagatcca gctcttcacc
    16081 ttctgcagtc ggctgaattt tgctcagcag tgcgcggtat tgatccagat cgctgctttc
    16141 cagctgctgc tgtactttag cggcgaattt ttccagacgg cgtttgccca gcagttctgc
    16201 gttcggcagt tctacttccg gaatagtcag cttcatagta cgttcaatgt tgcgcagcag
    16261 acgacgctcg cggttctcaa cgaacagcag cgcgcggcca gcacgacccg cacgaccggt
    16321 acgaccgata cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag
    16381 gctgatacgc tcaacgtcca ggccacgggc cgcaacgtcg gtcgcaatca ggatgtccag
    16441 acgaccgtct ttaaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt
    16501 cagcgcggcg ctattgtagc cgttacgctc aagagcttca gccacttcca gagtcgcgtt
    16561 tttggtacga acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag
    16621 tgcttcgttt ttacgcatac cccacacagt ccagtagctc tggctgatgt caggacgggt
    16681 agtcacgctg gactgaatac gcacttcctg cggctctttc ataaagcggc gggtaatgcg
    16741 acgaatcgct tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg
    16801 cgccataatg gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc
    16861 cagaaccaga ccgctcagtt tagagaggtc cagagtgcca cgtttcaggt ggtccagcag
    16921 acggcccgga gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc
    16981 ataacgctgg ccgccgtaca gggcaaccac atttacgccg cgcatgtgtt tagagaaatc
    17041 cgtcattgct tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg
    17101 tggtgctttc agctcaggat caagattctg cagcagaggt aaagagaatg ctgcagtttt
    17161 tccgctcccc gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca
    17221 ctctgcctga attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg
    17281 agccttcagg cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct
    17341 caaaattaat ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg
    17401 aaaagtgtga accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa
    17461 ggtgatggca atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat
    17521 tctgccaggt catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc
    17581 tcaacaaagt tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc
    17641 aaatccccca gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca
    17701 gcgagcgagg tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta
    17761 atgttgccca ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg
    17821 aagtgctgtt tcaatacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa
    17881 agccacagac tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct
    17941 tgcgctaact tgtcacgacc gccgtaatat aatgcaatcc cgcgattcaa gtgcgcgtag
    18001 ttgtaagttg gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg
    18061 cctgcctgcg ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt
    18121 gccagcgctt gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac
    18181 actccgcgct catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg
    18241 atttgttcca tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg
    18301 aggacttcac ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca
    18361 acgaaacacc agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa
    18421 cgtcctgttc ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg
    18481 gagggcaaat ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca
    18541 ggttgagact gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact
    18601 tccagaactt tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg
    18661 cgtttgtcag cgatttgaga gatgtgtacc agaccttctt taccgccgcc gatggcaaca
    18721 aatgcgccaa agtcaacgat acgggtcact ttaccagtgt agacgcggcc cacttcgatt
    18781 tctgcagtga tctcttcgat acgacgaata gcatgtttcg ctttctcgcc gtcggtcgct
    18841 gcaatcttca cagtaccgtc atcttcgatt tcgatggtgg tgccagtttc ttcggtcagg
    18901 gcacggataa cagagccgcc tttaccgata acgtctttga tcttgtccgg gttgatcttg
    18961 atggtatgga tacgtggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt
    19021 tccattacgc ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg
    19081 atctctttgg tgataccttc aattttgata tccatctgca atgcagagat accctcgcgg
    19141 gaacccgcaa ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac
    19201 agtacaacgt agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct
    19261 ttgatcggca cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa
    19321 gaggaaccgt tggattcagt gatttcagac acaacacgta cggtgtacgg gaatttgtcc
    19381 atatccggca tgactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga
    19441 cgcttcggag aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg
    19501 aacaggaagg tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca
    19561 gtacccagcg ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg
    19621 tgagtacgcg gcagcacgcc agtacgcaca tccagaccac ggatcatatc tttttcacga
    19681 ccgtcgatac gcggttcgcc tgccagtacg cggctacgaa caacgttttt ctcgatcgcg
    19741 tgcagaattt cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagcgtcgcg
    19801 atggtttcag atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg
    19861 taagcatcgc tcaggcgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt
    19921 accggctccg gctgccagtc ccaacgtggt ttaccggctt ctttcaccag ttcattgatg
    19981 ttctgaataa caacctgctg ttgttcatga ccgaacacta ctgcgcccag catctggtct
    20041 tcgctcagca gttcagcttc agattcaacc atcagtacag cggcttcagt accggcaaca
    20101 accagatcca gtttgctctc tttcaactcg tcctgagtcg ggttcagtac gtactggtca
    20161 ttgatgtaac ctacgcgagc agcaccaatc ggaccattga acggaatacc agacagagac
    20221 agtgctgcgg aagcaccaat catcgcgacg atatccgggt taacttgcgg gttaacagaa
    20281 accacggtgg cgataacctg aacttcgttg acgaagcctt ccgggaacag cggccgaatc
    20341 gggcggtcaa tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg
    20401 aagaagctac ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagt
    20461 gggaagaagt cctgacctgg tttggctttt ttctggccaa caacggtaac aaataccgcg
    20521 gtgtcatcca tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc
    20581 agagtcacgg tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata
    20641 tcctttctct ttcttagaca gtaccttacg gcactggtgt taatacccga tcttctgcgc
    20701 atcctcgcga ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc
    20761 gcgcgaacct ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt
    20821 gctgccgtca gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat
    20881 tagcgacgca gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc
    20941 aggtagtcga gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg
    21001 tgatcttttt tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt
    21061 gctacctgaa cttcagtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt
    21121 ttagctgttg cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg
    21181 acgccgatct ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg
    21241 cgacgttaag cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta
    21301 cgccgggtat tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc
    21361 cataccgata aatttgccgt tctcaccttc tgtaacgcga accagtcctt ccagcggcgc
    21421 accggatgta cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt
    21481 caccaccggg tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc
    21541 cgcagccgga atatcctgct gttcggcttg ttcgaccagt tcacgcagat gttccagggt
    21601 cacaatccgt tcaaccggat atttacttac cgccagacga cgcaggtaaa taacatgcgc
    21661 gccacaaccg agtttttcac ccaggtcatc aatgatggtg cggatataag tgccttttga
    21721 gcagtgaatt tccagctcca gctcattgcc ttcgtggcga ataaacagca attcataaac
    21781 ggtaatcggg cgcgcttcac gcggtacttc aatgccctga cgcgcatatt cgtacagttt
    21841 tttaccctga tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa
    21901 agtatccagc gccgccgcca gttgctctgc gctaaaggtt accggacgtt cttcaacgat
    21961 ttgtccgtcg gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg
    22021 tttgtcggag tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa
    22081 catgccagtt gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat
    22141 ccgtttcact ttttgcagcg catcattgct ggacataccc tgcggtttat ccagcaacaa
    22201 aacgccgtta atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg
    22261 tccgggttaa cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg
    22321 cgcatccctt caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg
    22381 cgcatcgctt tccccagcag gctgcggatg aaaccagaag cttcttgcaa cgctttgatg
    22441 cccgctttaa ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac
    22501 gccaggtcgc gagacatttc gacaccggaa acggtggtca tcatgcccag gcgaggatct
    22561 ttaatttcac gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc
    22621 gggcgaccaa attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc
    22681 tttttaaaat taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc
    22741 gtcacatcag gcaatccatg ccggatgcgg cgtaaacgcc ttatcccgca tggaacccta
    22801 aaaaccttaa gcgatggtac gttggatctc gatgatttcg aatacttcga tcacatcgcc
    22861 agtgcggacg tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc
    22921 gttaacgtca tctttgaagc ggcgcaggga ctccagctcg ccttcgtaga taaccacgtt
    22981 gtcacgcaga acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacagcc
    23041 tgcgatggca ccaaatttcg gcgatttgaa cacgtcacga acttccgcca gaccgataat
    23101 ctgctgtttc agttccggag acagcatacc gctcatcgcc gctttcactt cgtcaatcag
    23161 gttatagatg acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc
    23221 agaggcatca gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt
    23281 ggcgtcggtt tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc
    23341 agtagacagt ttcagcaagg agtcggagat cgcttccaca gaaccctgta cgtctgcctt
    23401 caggacgatg ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag
    23461 tttagatttc tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatacagtgc
    23521 aacttcacgc gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac
    23581 gccggacagg ccgaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc
    23641 cagttcgtta cgcatcgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc
    23701 cttgtgcaga gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag
    23761 gaaggattcg ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac
    23821 ttccgcctgc agcaggatag cgtccagcag ttcatcgata ccagtacccg ctttcgcaga
    23881 cacatgtacg aactggcttt caccgcccca ctcttccggc aggatgccgt actgggagag
    23941 ttcgttctta acgcgatccg gatcggcttc tggtttatcg atcttgttca ctgcaacgac
    24001 caccggaacc tgcgccgctt tcgcgtgctg gattgcttcg atcgtctgcg gcattacgcc
    24061 gtcgtcggca gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gagcacgcat
    24121 tgaagtaaac gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc
    24181 aacgtggtat gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt
    24241 tgaacgaatg tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac
    24301 aaccggcgcg cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc
    24361 cagctcgttt tcacgacgca ggataacttt atggcccatc tcttcagcaa ccagctgtgc
    24421 ggtttcctga tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt
    24481 gatgacctga gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatagtttc
    24541 gccgatcaca acgtcacggt taacggcctg agcaggcttc tggaagcctt gctgcagcga
    24601 agaaccttta cgttttccgc ctttaccgcc acgtactgct gcgcgtgctt cttcacgatc
    24661 agcttttgat tcagcgtgtt tgttgccttt cttcggacgc gctgctttcg cgttacgacc
    24721 acggccacgg ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg
    24781 agaagtagtg acgtgataat cgctggaatc ttcagtcggt tccgcgttat cagtccattt
    24841 gttttcttcc gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt
    24901 acgacgcgct tcttcttcag ctttacgctt gagctctgca gcttcctgct cacggcgggc
    24961 tttttcagcc tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc
    25021 cgcagcttca cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg
    25081 ttcagctttt tgttgcgcct cgcgtttagc cgattcttct gcctcacgac gggcttgctc
    25141 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    25201 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    25261 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    25321 caaagtctgt ttctcttgtg cagacacaga gtcgtcagca gacttccgga tacctgcatc
    25381 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    25441 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    25501 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca acccttcgat
    25561 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    25621 ggccagtttg aatgccaaat cacgatctac cccttcaagg ttcagcagat cgtcagccgg
    25681 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    25741 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    25801 catcggcaca taggccagtt cttccagcgt cgagaaacct tcttctacca gaacagtcgc
    25861 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    25921 ctgatgctta gcctgcaggt cgtcaacggt cattacgttg agttcccaac cgctcagctg
    25981 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccagcttc
    26041 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    26101 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    26161 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacgcaagc
    26221 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    26281 gtcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttccggca cttcaatacg
    26341 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    26401 ttccgggcga acggaataaa gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    26461 cggcagcata tcttcgcgca ggatcacggc ttcagcgttg ttgcccagat ccagagagat
    26521 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    26581 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    26641 cgtctgggtg gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    26701 caagttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    26761 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    26821 gatctgtaca cggacgtcga tctcttgctc atattttttc tttgttgctg tcgccagcgc
    26881 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    26941 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    27001 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    27061 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    27121 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    27181 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    27241 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    27301 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgcgaccgcg
    27361 aataaattcg atgcctacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    27421 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    27481 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    27541 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    27601 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    27661 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    27721 ccaccccgcg cctgaaacgt ggcaaattct actcgttttg ggtaaaaaat gcaaatactg
    27781 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    27841 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    27901 tgaatacttt tgccttcccc tgctctccct taagcgcatt attttacaaa aaacacacta
    27961 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    28021 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    28081 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttccggcggt
    28141 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    28201 actgcaaacc tgggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    28261 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    28321 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    28381 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    28441 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    28501 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    28561 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    28621 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    28681 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    28741 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    28801 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcgacgacgt agaaatgatg
    28861 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    28921 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    28981 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    29041 gcgcatggtc gtcagttggg ccgtctgctg taccaggggc gttggtttga ctcccaggcg
    29101 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    29161 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    29221 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    29281 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    29341 tatgccaaaa ctggcctgct ttcctcctct gccacttcag gcctgccgca ggtggagaat
    29401 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcaggggtg gggttacctt
    29461 gcttatcctc tgtaaactcc tttctgagca tcacataatc gaaaacatca tggtttgcat
    29521 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    29581 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    29641 tattgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    29701 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    29761 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    29821 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    29881 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    29941 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    30001 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    30061 ccatggcgcg catggcgata ctggtaactg ctgtaccatt ctgccgaaaa gcggattgcg
    30121 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    30181 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    30241 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    30301 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    30361 actcccccac aatcaacacg taggtatcaa tacttgtatc cctgactgat aattgaaaat
    30421 acggcaccgt gtttgcaatt gagagtaatc tttgatgcgc tttcgctgct aaagcaaaat
    30481 agttgagatt gaaaaacggc gtataggtag caaatcacga cgctagtata tatggactga
    30541 acgctttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    30601 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    30661 ttattattac tgccaaaaaa agaagggata aaaaagcaaa agcacataga taagggatat
    30721 acatccccag cattttcaca acttcatccg gatcactctg cagcacacta atcgcaaatc
    30781 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    30841 cagtcagtaa cacaaatggt gcggcaacaa gcaggcgaag cggaagggat gatatacgtt
    30901 taacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gctccggaag
    30961 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    31021 taaaatcttc tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat
    31081 ataaacaatt atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat
    31141 atcagaccga aacactgtta gttttttctt tttaaatgat gcgaccattg cgttctcagt
    31201 aattaaataa cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt
    31261 atactgacag gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac
    31321 tggagttgac acaataaagt gccaattatg tcagcagtag ggaaaagttt ataactaagg
    31381 cgtaccggca ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag
    31441 atacctgaca accattcatc agaccaggac caaaacgaaa agagacgctt ttcagcgtct
    31501 cttttctgga atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac
    31561 cacctcaagg tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt
    31621 cgggatatcg ctggtcggct tagccggagc agctggctga gtttgttcgg ttttcgccgg
    31681 tgcactcaga ttttcccatt cgctacctct attggttttg ttgctattga tgttacccag
    31741 caccagactg atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt
    31801 accagaacca cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat
    31861 atcagcgcct ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag
    31921 gaaaactact aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac
    31981 caatgcttca accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata
    32041 ctaaccaaaa cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa
    32101 aaaacagcaa aaccgatgtg ttcgccgaga aaaaaggcga caatcgccgc ctttttagcc
    32161 agttatctaa cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc
    32221 acctgcgctt cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta
    32281 cgcagcaaca cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta
    32341 actgactcat gctcaagcgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc
    32401 tgcgggaaca ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc
    32461 gccgccagca cctgcaagcc agcaacgatg ccgtcaccgg tagtggtttt atccagcagg
    32521 atcacatgac cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt
    32581 acgtagcggt cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt
    32641 tcaagcccca tgttgctcat caatgtaccc acagcaccac cacgcagctg gccctgacga
    32701 agaccttcac gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg
    32761 tcaaccataa tcacgcgatc gccgtcgccg tcgaaggcaa taccgagatc cgctttttca
    32821 gccagcacac gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt
    32881 acaccgtttg gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc
    32941 ggcgcgatgt gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg
    33001 ctaagttcgt tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg
    33061 atacggctgg ctttacccag ttcagccgaa tcaacgcagc tgatctcctt ttccatttcc
    33121 gcttcgatgg cctcttctac cgcatccggc agtttagtgc cgtcgataga gaagaattta
    33181 atcccattat cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg
    33241 aaggtacgcg tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa
    33301 aggcccgctg ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc
    33361 gtgtctttac caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc
    33421 caacccagct taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc
    33481 ccatcggtac cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt
    33541 ggcttccacc acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg
    33601 cgcgccttgc attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg
    33661 ccccacgttc agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag
    33721 gttgaaatgg tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt
    33781 accgaaaccg aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca
    33841 acgtgctatt tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg
    33901 agcttcctgc atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt
    33961 ttctgcagcc gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg
    34021 agcgccaact ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca
    34081 gacttcgaag cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc
    34141 aacgctaact tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc
    34201 gccagcattg atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc
    34261 accatccgaa aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag
    34321 gtccagtgaa gtaccctggg caaagagttt cattatgtta tccctggtat gaattgataa
    34381 gaaaaaaccc cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga
    34441 acttacttgt cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca
    34501 tcaaccggac gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt
    34561 tcttcccagc ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt
    34621 gcgtcgatag tctcatattt catgagagca tctttcatcg catgcagaat atccatattg
    34681 tcggtcagaa gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg
    34741 tcgatgatac gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg
    34801 aatacttcac cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac
    34861 tgagtcacca tgttacgtgc caggttggtc gcaactttaa tatcgttgga cgcaccggta
    34921 gaaacatgtt ccggcccgta gatgatctct tctgccagac gaccaccgta cagcgtagaa
    34981 atctggcttt ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa
    35041 gtcacaccca gcgcacgacc gcgtgggata atcgtcactt tatgcaccgg atcgtgttcc
    35101 ggcaccaggc gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc
    35161 gcttccgtca tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc
    35221 tcgaactcaa ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg
    35281 ttcaccaggt tcgccaggtc agcaccggag aaaccaggag taccacgagc aatgattgcc
    35341 gcgtcgatat cgggtgccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga
    35401 ccgcgaacat ctggcaagcc gaccacaacc tggcggtcga aacggccagg acgcagcagg
    35461 gccgggtcga gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct
    35521 tcgaagccat ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca
    35581 ccgcccagac cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg
    35641 cacggtgccg ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg
    35701 acgaacattt ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct
    35761 gcaatcgctt tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct
    35821 ttcgggatct taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt
    35881 tcagcaactt cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc
    35941 tgatcttccg tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg
    36001 ccgccctgca tttgacgcat gaagaagatc cagacaccaa tcagcaacag catcgggaac
    36061 caggagatga agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg
    36121 acgttcttgg tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta
    36181 taacggttac tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga
    36241 acctggtcgt tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac
    36301 tcgctgggcc caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt
    36361 attaggtttt tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac
    36421 agcgtcagga tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag
    36481 ggtttacgcc cggtcgctac aatatacact tctcgcgaac gtgcacgaga agagtccggc
    36541 ttacgaactt tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct
    36601 tcgccctgga acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt
    36661 tctagcgcca gttccaccag atacatggct cgggggatat ccaccgccgg tgttccgctc
    36721 atgtttggtg ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc
    36781 agtgctttca tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata
    36841 ggatccatag gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc
    36901 acatattgtg accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta
    36961 aagagtttgt cactttgctg tatttcatca agtttaaacc aggcacggga acgtaatccc
    37021 tttttctgtg cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag
    37081 ctggcagaac gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca
    37141 attgcgtaaa tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc
    37201 gttttcaatc ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca
    37261 cctgaaaggt ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac
    37321 cgaaggggtg ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa
    37381 aatcgccacc gaagatcgcg aaacaaaaac cttgatcgtg gaagctatcg tgcgcgaaac
    37441 cggcgcctgt aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga
    37501 acgtaaaatc tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa
    37561 acgaggggtt ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc
    37621 cgcagagcgg cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc
    37681 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    37741 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    37801 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    37861 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    37921 tttggtgaca tcaatcacct gcgcgtttga cagcttggct tcgatgtctt tgatacggcc
    37981 ttcgcagaag ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    38041 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    38101 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    38161 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    38221 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    38281 aggtcagcta caattcacat tttgatggtc attttaccct gaagttcccg aagggtcatc
    38341 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    38401 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    38461 ggccgcgaat gttgatgagt acgttactca actccccgca ggtgccaacc ttgccctgat
    38521 ggtgcaaaaa gtcggtgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    38581 gcccgccagt acccagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    38641 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    38701 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    38761 ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    38821 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    38881 ctttagcgct ccgcctgctg ccgccatagt tgaccgcaac tgtttctccg tctcgctcta
    38941 cagtgcccca aagcctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    39001 gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat actgcgaact
    39061 ggatgtggtg ccaagcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    39121 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gtgcaattct
    39181 gaaagatgag ttaaaacagg cgggtatcac ctggagcgga acactgctgc gccagactca
    39241 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc acgatctgct
    39301 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatcgg
    39361 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggctggg tcggacgccg tgcgtcagat
    39421 cctgcgccag caagccggtg tcgatattgg taacaccatt attgccgatg gttcagggct
    39481 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat acattgccca
    39541 acacgacaat gaacttaact ttatctccat gctgccgctt gcgggctatg acggctcttt
    39601 gcagtaccgt gcaggtctgc atcaggcggg cgtggatggc aaagtctcag cgaaaaccgg
    39661 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    39721 ggcatttgta caatatcttt ctggctatgc agtaaaaccc gcggatcagc gtaatcgccg
    39781 tattccgtta gtgcgttttg aaagccgttt gtacaaagat atttatcaga acaattagtc
    39841 aaaaagaaac cccggcgcat ggccggggtt tcagattatt gacaaagtgc gctttgttta
    39901 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat cgtgcaaatt caatatattg
    39961 caatcatctt gtaggcctga taagtgtagc gcatcaggct tatttggcat tggtcattag
    40021 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcgtcatc
    40081 ccagtcttca tcatcctctt cagcaatctc ttcaagctgc tgacggtgat aatcatccca
    40141 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    40201 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccgctcg ccgcagagat
    40261 cagataatat ttatcttccc agcccagcgc ttcagcgatc gctttcgctt tctcttcggc
    40321 ttctgcctta tccagcagat cgatcttgtt gaagactaac caacgcggtt tcgccgccag
    40381 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    40441 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    40501 caggaagcga atgcccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    40561 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    40621 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcacgaa taaaggtcga
    40681 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    40741 cagctcacgc ttatcgcccg gcgtaccgtt agttttctgc cgtggtgtac ggttaactga
    40801 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    40861 accgtgttta gtcatatcac ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    40921 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    40981 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    41041 gttctcgtcg gcttccatcc atacgtcacc accatcaccg ccgtcgccgc catccgggcc
    41101 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    41161 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    41221 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    41281 aatggcggaa tacatcgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    41341 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    41401 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    41461 cgcttccgcc aaggcgccat atcctaccaa ggtattcagt ccgcaaaaaa ttaaacatgc
    41521 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcgat
    41581 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    41641 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    41701 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    41761 cagaccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    41821 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    41881 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    41941 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    42001 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    42061 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    42121 ccagcaaatt gctgtggtaa gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    42181 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    42241 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    42301 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    42361 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    42421 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    42481 accgaaacgc ttaacgccca agcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    42541 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    42601 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    42661 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    42721 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    42781 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    42841 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    42901 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    42961 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    43021 aaacacaact tgtacggtaa cgtttatctg tgccattttt tcagtacaat taccctatat
    43081 tcctaaccat aaaccctaag ttgcctttgt tcacagtaag gtaatcgggg cgaaaagccc
    43141 ggcttttgcg atgaatttag aaaaaatcaa tgagttaacc gcgcaagata tggcgggtgt
    43201 taatgcggca atccttgagc agcttaattc cgacgtccaa ctgatcaatc agttaggcta
    43261 ttacatcgtc agcggcggcg gtaaacgtat tcgtccgatg attgctgtac tggctgcacg
    43321 agctgttggc tatgagggaa atgcgcatgt caccattgct gccctgatcg agtttatcca
    43381 cacggcgact ctgctacacg acgacgttgt ggatgaatca gatatgcgca ggggtaaagc
    43441 taccgccaac gccgcatttg gcaatgccgc cagcgtgctg gtaggcgatt ttatttatac
    43501 ccgcgcgttc cagatgatga ccagcctcgg ttcactcaaa gtgctggaag tcatgtcaga
    43561 agctgtaaac gtcatcgcag aaggtgaagt tctgcaactg atgaacgtta acgatccgga
    43621 catcactgaa gaaaactaca tgcgcgttat ctatagcaaa accgcgcgtc tgtttgaggc
    43681 tgccgcgcag tgttccggga ttctggctgg ctgtacgccg gaggaggaga aaggcctgca
    43741 ggattatggg cgctatctcg gcactgcttt ccagttgatc gacgatttac tcgattacaa
    43801 tgccgatggc gaacagttag gtaaaaacgt cggcgacgat ctgaacgaag gtaaaccgac
    43861 gctgccgctg ctgcatgcga tgcatcatgg cacaccagaa caggcacaga tgatccgtac
    43921 cgccatcgaa caaggtaacg gtcgccatct tctggaaccg gttctggaag caatgaacgc
    43981 ttgtggatct cttgaatgga cgcgtcagcg cgccgaggaa gaagcagaca aagccatcgc
    44041 agcgttacag gtgctcccgg acaccccttg gcgagaagca ctcatcggcc tcgcgcacat
    44101 cgctgttcaa cgcgatcgtt aatcccctcc cttcatcccg cgcagtgcgc gggatgattc
    44161 cagtaaattc cataaaaaca ctaattcagc gctataacac ctgaaaaaag ggcgatgaat
    44221 ctttatatat tctgaatatt cacactcttt acaggaactt tttagcgcaa tagaccatca
    44281 ggagtatagt gatgcccgac agaagaagtg ttctgaatga aagcgactaa cttaaggagt
    44341 gaggaaagat ggaaagtaat ttcattgact ggcatcccgc tgacatcatt gcaggtttgc
    44401 gcaagaaagg aacgtcaatg gcggcggaat ctcgcagaaa tggtttgagt tcctcaacgc
    44461 tggcaaacgc attatcgcgc ccatggccga aaggagagat gattattgcg aaagccctgg
    44521 gaactgaccc ctgggttatc tggccatcac gctaccatga tccgcagacc catgagttta
    44581 tcgacagaac gcagttgatg cgtagctaca ctaaaccgaa aaaatgagtg gtctggcggt
    44641 agccccgcga acggggctgc cagctctcag acgattactc gcctttcaca cgctcaatat
    44701 ttgcacctaa agcgcgcagt ttgtcttcaa tgcgttcgta gccacgatcg atgtgataaa
    44761 tacgatcaac caccgtcgtc ccttccgcaa tacagccagc cagcaccagg cttgctgatg
    44821 cacgcagatc ggttgccata acctgtgcac cagaaagttt ttcaacacca tgacaaataa
    44881 cggtattgct ttcgatttcg gcgtgcgcgc ccatacggct cagctctggc acatgcataa
    44941 agcggttttc aaagaccgtt tcggtgatga acccggtccc ttcagccacc aggttcaaca
    45001 gcgtgaactg ggcctgcata tcggtcggga atgccggatg cggcgcggta cgtacgttaa
    45061 cagccttcgg acgtttgcca tgcatatcca ggctaatcca gtcttcgccg acttcgatgt
    45121 ccgctccagc atcacgcagt ttcgccagca cggcgtccag agtatctggc tgcgcgttac
    45181 ggcagataat tttgccacga gagatcgccg ccgccaccag gaaagtaccg gtttcgatac
    45241 gatccggcag cacgcgatag acaccgccgc ctaaacgttc cacaccttcg atgacgatac
    45301 gatcggtgcc ctgaccgcta attttcgcac ccagcgtaat caggaagttc gcggtatcga
    45361 cgatttccgg ttcacgcgct gcgttttcaa taatcgtggt gccttccgcc agggttgcag
    45421 cacacatgat ggtcaccgtt gcgccaacgc tgactttatc catcacgata tgtgcgcctt
    45481 tcagacgacc atcgacggaa gctttaacgt aaccttcttc cagtttgatg gtcgcgccta
    45541 attgttcgag gccagaaatg tgtagatcaa ccggacgcgc accgatcgta caaccgccag
    45601 gtagtgaaac ttgcccctga ccaaagcgcg ctaccagcgg ccccagcgcc cagatagaag
    45661 cacgcatggt tttaaccaga tcgtaaggtg cgcagaatac attaacgtcg cgggcatcaa
    45721 tatgcacaga accattacgt tctactttcg cacccagctg gcttagcagt ttcattgatg
    45781 tatcgacgtc tttcagtttc gggacgttct ggatctctac cggttcttcc gccagtagtg
    45841 cggcaaaaag aataggcaga gcagcatttt tagcgccgga aattgtgact tcgccctgga
    45901 gcttcgttgg cccctgaaca cgaaatttat ccatttagtt tgttctcagt taacaattca
    45961 tatccgctac cggcgaatcg cccatagctc aaaagccgtt cagtttgcga tcgcgcgccc
    46021 actccgcagg ggtatacgct ttgatcgaca cagcatgaat gcggttatcc gcaatatatt
    46081 ccatcagcgg gccatagacc gtctgctgtt ttttaacccg actcataccg tcaaacaact
    46141 cacccacggc aataacctga aagtggctgc catcgccgga aacgtggact tcctggaggg
    46201 agagagcgtt catcaacacg ctctgaattt cattattttc catgggatct tcaatcatca
    46261 gttaataaac cagcgaaaca tcttagagca aagttgcgct ggcataaata agcaaaaagc
    46321 ctcgctgata aatcagacaa ggctcgactt gcaggcaggt ttgccggaca ggcggttaac
    46381 gccatatccg gcctgaaaaa atttaacgag gcagaacatc agcaggcaaa ttatacaatt
    46441 tcgcgagggt atacactttg tcgtttaccc cctgaagcgt cacgttgttg ccctgctttt
    46501 tcgccagatc gataagatgg agcagcagtg ccagtccccc cgtatccacg cgggacacac
    46561 ggctaagatc gatgcaggta atccccttca ccgcctcctc acgcatttcc caaagcggta
    46621 gcagaacgtc ctgatccagc tctccggata acgccagcgt gtcacccgtc tgcatccagc
    46681 tcagtgactc gctcattatt ttttctcttc cagagtgatt ttctgttgag aaatcgattt
    46741 cagttgcgca gtcaggccgt cgataccttt ggtacgcagc agcgttcccc actcgttttg
    46801 tttggtggtg atcatactga cgccttcagc aatcatgtcg taagcttgcc agtttcccgt
    46861 ctgggagttt ttacgccact ggaagtccag acgcaccggc ggacggccat tcgggtcaat
    46921 aatggtaacg cgaataggca caatggtttt atcgcccagc ggctgttctg gcgcaatctg
    46981 ataggtttga ccgtgataca tcgccagcgc ctgaccgtaa gcctgcttca ggtactcacg
    47041 gaaagcggca aagtaggctt cacgttgagc aggggtcgca ctcttgtaat actggcccag
    47101 caccagcgca ccggcgtatt tcacctgtac gtatggcagc agttcctgat caacaatggt
    47161 acgcagataa tccgggttgg cccgaatttg cggttgctca ttcttcaggc gatcgaacgt
    47221 tttctgcgcc gcctcgtcca tcagcttata aggattggtc tggtctgccg cggttgccgc
    47281 actcagaggt gcaatcacca gcaaagcgac catcattaaa cgtttaaaca tgcgtcggtt
    47341 ctcctgaaat tatttcgttg tacccacagg ttcagtggtt tcattattac ctggcgcagc
    47401 agctggcgca tcgccactat tcttattgtc atcgccttta ctaccgtaaa ggaactgacc
    47461 aatgagatct tccagcacca tcgcagactt ggtgtcctga attgtatcgc catccttcag
    47521 gatagcagtc cccagttccg ggtcttcaaa accgacgttt aatgccagat attgttctcc
    47581 cagcaggccg gaagtacgaa tgctcagcga actggtatca ggaatgtggt tataacgttg
    47641 ttcaatttcc agcgttacgc gcggcagata ggttttcggg tccagcgtaa tatccgccac
    47701 ccggcccaca acaacaccac caatgctgac cggagagcgc gctttcaggc cgccaatgtt
    47761 atcgaacgtc gcataaagcg tgtaggtcga ttcagtacgt atggacgtca cgttcgccgc
    47821 cttcaggcaa acaaacagcg ccgccagcag tgctgctaat aaaaagatac ccacccaaat
    47881 ttcatttttt ttcgtttgca tgaactcaat tcccaaacat caatgcggtc agcacaaaat
    47941 ccagccccag aacagccaga gacgagtgga caacggtgcg agtggttgcc cggctaatcc
    48001 cggcagacgt cgggatggcg tcgtagccgt taaacaacga aatccacgtc accgtgatgg
    48061 cgaacaccac gctcttaatc agacagttga ccagatccat acgccagtcg acggcatttt
    48121 gcattgccga ccagaagaac ccgctatcaa tgcctttcca actgacgccg accagcgatc
    48181 cgccccagat ccccacggcg acgaagataa ccgtcaacag tggtaatgaa ataaccccag
    48241 cccagaaacg gggagaaata acccgacgca gcggatccac cgccatcatc tccatactgg
    48301 agagttgctc tgtagcgcgc atcaggccga tttctgcggt taacgccgaa ccagcacgcc
    48361 cggcaaacaa cagcgcggca accaccggcc ccagttcacg cagtagcgat aacgccacca
    48421 gcatacccag actggtttcc gcgctataag tggtcagaac cagataacct tgcagcccca
    48481 acaccattcc gatgaacacg ccagaaacta caataatcag catcgacagg acgccgacat
    48541 tatagagctg gcgcaccagc agcggcgcat gtttgcgaaa ttccggtttg ccgaccagcg
    48601 cattgaataa cattaacccg gcccgcccga acgttctcag ggttttaatc cctttatgtc
    48661 cgagcgacgc cagcgcattt aacagcatga gtggcttaac tccctggtaa aagatcagcg
    48721 tgataatcgc cggcaggata gcggaacgga acaggcccgt cagctatccc gtccagaaac
    48781 tgacgtacgc gcggatcagg attcgtctgc aacgcctggg cactgccatg agcgacaatt
    48841 tttttgtccg ccaggatcca ggcgtgatcc gcaatactta acacttccgg cacatcgtga
    48901 gaaaccacca cacaagtcac gcccagcgcg ctgttcagct cagaaatcag cttcaccagt
    48961 acgcccatgg tgatgggatc ttgcccaaca aaaggttcat caaacatgat gagatccggc
    49021 tccagcgcaa tcgcacgcgc cagcgctgca cgccgcgcca tcccaccgga aagttcagaa
    49081 ggcattagtt tagccgctcc acgcagcccc acggcctcca gcttcatcat caccgtacta
    49141 tgcaacaatg gcgcgggaag ttgggtatgt tcgcgcagtg gataggcgac gttgtcaaat
    49201 acgttcatat cagtgaacaa cgccccggac tgaaataaca tgctcatccg tttacgcact
    49261 gtatacaggc gcgaacgaga catcgccgga atattctcac catcgaaaag gatctcacca
    49321 tgatctggtg cgatttgccc gccaatcaga cggagtagcg tcgttttacc gatgcccgat
    49381 ggccccatga tcgccgtgat cttccctcgc ggcacggtca gggaaatatt atcgaagatg
    49441 cagcgattgc cacgcgtaaa actgacatcg cgcatatcga ctaaattcgc cacagactgc
    49501 tccataattc acccttcgtc ttgcgttgat tttctaagca tggcgctcaa tttaaccttg
    49561 aacccaacat atttacagaa tattacccgc cgtggttagc gaaagctggc atttgtttta
    49621 ctttttagcc gcataaagtc aaaattaaga atccgttacg gctttctgaa aatcttcagc
    49681 ggaccggcga gtatacctga agaaaggacg ttagatgctt ttagctacgg cactgttaat
    49741 tgttggttta cttttggtcg tttacagtgc cgaccgcctg gtttttgccg cgtctattct
    49801 ttgccgaacc tttggcatcc cgccgctgat catcggcatg acggtggtca gtattggtac
    49861 atcgttacca gaaatcatcg tctcgcttgc cgcgtctctg cacgaacaac gcgatttagc
    49921 cgttggtaca gccctcggct caaacattat caatatattg ctgatcctcg gtctggctgc
    49981 gctggttcgt ccttttaccg tccattccga tgttctgcgc cgtgaattac ccttaatgtt
    50041 gttggtcagc gtggtggccg gttccgtact ctatgacgga caacttagtc gaagcgatgg
    50101 tatctttctc ctctttctgg ctgtgctatg gctgctgttc attgttaaac ttgcacgtca
    50161 ggggactgac agcctgacca gagagcagct tgcagagctg ccgcgtgacg gcggattgcc
    50221 cgtcgcgttt ttatggctcg gcattgcgct tatcatcatg ccagtggcca cgcggatggt
    50281 ggttgataac gccacggtgc tggcgaatta ctttgccatc agcgagttga cgatggctct
    50341 gacggcaatt gctatcggaa ccagtctgcc ggaactggca accgcaatag cgggggttcg
    50401 caaaggtgaa aacgacattg ctgtcggaaa tatcattggc gcaaacattt ttaatattgt
    50461 catcgtgttg ggtttacccg cgctgataac gccaggagag attgatccac tggcgtacag
    50521 tcgtgactac agcgtgatgt tgctggtgag cattattttt gcgttgctgt gctggcggcg
    50581 ctccccgcaa ccgggccgtg gtgtaggggt attattaact ggcggattta tcgtatggct
    50641 ggcgatgttg tactggttat cgccaatact cgttgaataa ctggaaacgc attatgtcgc
    50701 acgtagagtt acaaccgggt tttgactttc agcaagcagg taaagaagtc ctggcgattg
    50761 aacgtgaatg cctggcggag cttgatcaat acatcaatca gaatttcacg cttgcctgtg
    50821 aaaagatgtt ctggtgtaaa gggaaagttg tcgtcatggg gatggggaaa tcggggcaca
    50881 tcgggcgcaa aatggccgca acgtttgcca gcaccggtac accttcattt ttcgtccatc
    50941 ctagtgaagc cgcgcatggt gatttaggca tggtcacccc acaggatgtg gtgattgcta
    51001 tctctaactc aggtgaatcc agcgaaatca cggccttaat tccagtgctt aagcgtcttc
    51061 acgtaccgtt aatctgcatc accggtcgcc cggagagcag catggcgcgc gccgcagatg
    51121 tgcatctgtg tgttaaagta gcgaaagaag cctgtccgtt agggctggca ccgaccagca
    51181 gcaccaccgc cacgctggtt atgggcgatg ccctcgctgt cgcgctgtta aaagcacgcg
    51241 gctttactgc tgaagatttt gcgctctcac acccaggcgg cgcactgggt cgtaaacttc
    51301 tgctgcgcgt aaacgatatt atgcatacgg gcgatgagat cccgcatgtt aagaaaacgg
    51361 ccagtctgcg tgacgcattg ctggaagtta cccgcaaaaa tcttggtatg actgtcattt
    51421 gcgatgacaa tatgatgatt gaaggcatct ttaccgacgg tgatttacgc cgtgtcttcg
    51481 atatgggcgt ggatgttcgt cagttaagta ttgccgatgt gatgacgccg gggggaatac
    51541 gtgtgcgccc tggcattctg gccgttgagg cactgaactt aatgcagtcc cgccatatca
    51601 cctccgtgat ggttgccgat ggcgaccatt tactcggtgt gttacatatg catgatttac
    51661 tgcgtgcagg cgtagtgtaa agattcaagg ataaacaaca atgagcaaag caggtgcgtc
    51721 gcttgcgacc tgttacggcc ctgtcagcgc cgacgttatg gcaaaagcag agaacattcg
    51781 tctgctgatc ctcgacgttg atggcgtact gtcagatggc ctgatttata tgggcaataa
    51841 cggcgaagag ctgaaagcgt tcaatgttcg tgacggttat ggcattcgtt gtgcgctcac
    51901 ctctgatatt gaagtcgcta tcattaccgg gcgaaaggct aaactggtag aagatcgttg
    51961 tgccacattg gggatcactc acttgtatca ggggcagtca aacaaactga tcgcctttag
    52021 cgatttgctg gaaaaattgg cgattgcccc ggaaaatgtg gcttatgttg gcgatgatct
    52081 catcgactgg ccggtaatgg aaaaagtggg tttaagcgtc gccgtggccg atgcgcatcc
    52141 actgttgatc ccgcgcgccg attacgtgac gcgcattgct ggcggtcgtg gcgcagtgcg
    52201 cgaagtttgc gacttattac tcctggcgca gggcaaactg gatgaagcca aagggcaatc
    52261 gatatgagta aagccagacg ttgggttatc attgtgctat cactggcggt tctggtgatg
    52321 atcggcatta atatggccga aaaagacgat accgcccagg tggtcgtcaa caacaatgat
    52381 cccacctata aaagcgagca tacggacacg ctcgtctata acccagaagg ggcactaagc
    52441 tatcgattga ttgctcaaca cgttgaatat tattccgatc aggccgtttc gtggtttacg
    52501 cagccggtac ttaccacgtt tgataaggat aaaatcccga catggtccgt aaaagcagat
    52561 aaagccaagc tgaccaatga ccggatgctc tatttatatg gacacgttga agtcaacgca
    52621 cttgtgccag actctcaact tcgcagaatc acgacggata acgcgcagat caatctggtg
    52681 acgcaggatg ttacctctga agacctcgtc acgttatacg gaacaacatt taactccagc
    52741 ggtctgaaaa tgcgcggcaa cttacgcagc aagaacgccg agctgattga aaaggttaga
    52801 acatcctatg aaattcaaaa caaacaaact cagccttaat cttgtgcttg ccagctcact
    52861 tctggccgcc agcattccgg catttgccgt aaccggagac actgatcagc cgatccacat
    52921 tgaatcggac cagcaatctc ttgatatgca aggcaacgtg gttaccttta ccggtaatgt
    52981 catcgtcacc cagggcacca tcaaaattaa tgccgacaaa gtggtcgtta cccgtccggg
    53041 cggcgaacaa ggtaaagaag tgattgacgg atacggtaaa ccggcaacgt tctaccagat
    53101 gcaggacaac ggtaaacccg ttgaaggtca cgcttcccag atgcactacg aactggcaaa
    53161 agattttgtc gttctgacgg gtaatgctta tctgcagcag gtcgatagca acattaaggg
    53221 cgataagatc acttacctgg tgaaagagca gaaaatgcag gctttcagcg acaaaggcaa
    53281 gcgcgtaacc accgttctgg tgccgtcgca gctgcaggac aaaaacaaca aaggccagac
    53341 cccggcacag aagaagggta attaattcgt tatggcaaca ttaactgcaa agaaccttgc
    53401 aaaagcctat aaaggccgtc gcgtggtaga agacgtcagc ctgaccgtca actccgggga
    53461 aattgtcggt ctgctggggc caaacggtgc cggtaagacc accactttct acatggttgt
    53521 aggcattgtg ccgcgcgatg cgggcaacat cattattgat gatgacgata tcagtctgct
    53581 gcctctgcat gcacgcgcgc gccgcggtat cggctatctg ccacaggaag cctccatttt
    53641 ccgtcgcctc agcgtttacg ataacctgat ggcggtactg caaattcgtg acgacttgtc
    53701 tgctgaacaa cgtgaagacc gcgcgaacga gctgatggaa gagtttcaca ttgagcacct
    53761 gcgtgacagc atggggcagt cactctccgg gggtgaacgt cgccgtgtag aaattgcccg
    53821 cgcactggct gcgaatccga aatttattct gctcgacgaa ccgtttgccg gggttgaccc
    53881 gatctcggtt atcgacatta aacgcatcat tgaacacctg cgcgacagcg gcctgggcgt
    53941 gctgatcact gaccacaacg tgcgtgaaac actggcggtt tgtgaacgcg cttatatcgt
    54001 cagtcagggg catttgatcg cccacggcac gcctacagaa atcttacaag acgaacacgt
    54061 taagcgtgta taccttgggg aagacttcag actctgatag ggtagaagtt tgcgacgttt
    54121 tagcaggaga gtacgattct gaacatgaag caaggtttgc aactcaggct tagccaacaa
    54181 ctggcgatga cgccacagct ccaacaggca attcgtctgt tgcagttgtc gacgctggaa
    54241 cttcagcagg agctacagca ggcgctggag agtaatccgc tgcttgagca aatcgacact
    54301 catgaagaaa tcgacacccg cgaaacgcaa gacagtgaaa cgctggacac cgccgacgcg
    54361 ctcgaacaaa aagagatgcc ggaagagctg ccgctcgatg ccagttggga caccatttat
    54421 accgctggta caccatccgg caccagcggt gactacattg acgacgagct gccggtctac
    54481 cagggcgaaa cgacgcagac cttgcaggat tacctgatgt ggcaggtcga gctgacaccg
    54541 ttttccgaca ctgaccgcgc tattgctacc tctatcgtcg atgccgttga tgaaaccggt
    54601 tatctgactg tcccgctgga agatattctc gaaagtatgg gcgatgaaga gatcgacatc
    54661 gacgaggttg aagccgtcct taagcggatc caacggtttg acccggtcgg tgtagcggca
    54721 aaagatctgc gtgactgcct gctaatccaa ctctcccaat tcgataagac cacgccgtgg
    54781 ctggaagagg ccagattgat cattagcgat catctcgatc tgttagccaa tcacgacttc
    54841 cgcactttaa tgcgcgtcac gcgtctgaaa gaagatgtgc tgaaagaagc cgtcaatctg
    54901 atccagtcgc tcgatccgcg ccccgggcag tcgatccaga ctggcgaacc tgagtatgtc
    54961 attccagatg tgctggtgcg taagcataac ggtcactgga cggtagaact caacagtgac
    55021 agcattccgc gtctgcaaat caaccagcac tacgcctcga tgtgcaataa cgcgcgcaac
    55081 gatggtgaca gccagtttat ccgcagcaat ctgcaggatg ccaaatggtt gatcaagagt
    55141 ctggaaagcc gtaacgatac gctactgcgc gtgagtcgct gtatcgttga acagcagcaa
    55201 gccttctttg agcaaggtga agaatatatg aaaccgatgg tactggccga tatcgcccag
    55261 gctgtcgaaa tgcatgaatc gacgatatct cgcgtgacca cgcaaaaata cctgcatagt
    55321 ccacgaggca tttttgaact gaagtatttc ttttccagtc acgtcaatac cgagggcggc
    55381 ggcgaagctt cctccacggc gattcgtgca ctggtgaaga aattaatcgc ggcggaaaac
    55441 ccagcgaaac cgttgagcga cagcaagtta acctctttgc tgtcggaaca aggtatcatg
    55501 gtggcacgcc gcactgttgc gaagtaccga gagtctttat ccattccgcc gtcaaaccag
    55561 cgtaaacagc tcgtttgacc caaccgataa ggaagacact atgcagctca acattaccgg
    55621 aaataacgtc gagatcaccg aggcactgcg cgaatttgtt acagctaaat ttgccaaact
    55681 tgagcaatat tttgaccgaa tcaaccaggt ctatgttgtt ctgaaagtgg agaaagtcac
    55741 ccacacctca gatgcaacac tgcatgtaaa cggtggtgaa attcatgcca gcgcggaagg
    55801 tcaggacatg tacgctgcca ttgatggctt aattgataag ctggcacgcc agttgaccaa
    55861 acataaagat aaactgaaac aacactaatt gtccgggcaa ttagcatgtg catggcggcc
    55921 tgttgtgcgg cacaacgggc catttgtacg gttaatgctc cgagcctgtt ccactgtttg
    55981 agtgggcagg ttcttaggtg aaattatgac aaataatgat acaactctac agcttagcag
    56041 tgttcttaac agggaatgta cgcgaagccg cgtccactgt cagagcaaaa aacgcgccct
    56101 ggaaatcatc agcgagctgg cggcgaaaca acttagcctg ccgcctcagg tggtttttga
    56161 agctatcctg acgcgggaaa aaatgggcag taccggtatc ggcaatggta ttgccattcc
    56221 gcatggcaaa cttgaagaag atactctgcg tgccgttggc gttttcgttc agctcgaaac
    56281 gcctatagct ttcgatgcca tcgacaacca accggtggac ttactttttg ccctgctggt
    56341 gccggcagac caaactaaaa cgcacctgca tactctgtcg ctggtggcga aacgtctggc
    56401 ggacaaaacc atctgccgcc gtttacgcgc agcccagagc gatgaagagc tgtatcaaat
    56461 cattacggat accgaaggta ctccggatga agcgtagtta ttcggtaatg tctcttttag
    56521 acgttgtgag gagaaacagt acatggtact gatgatcgtc agcggacgtt caggttcagg
    56581 taaatctgtc gccctgcgtg cgctggaaga tatgggtttt tactgcgtgg ataaccttcc
    56641 cgtagtgttg ttacccgatc tggctcgaac tctggccgat cgcgagattt ctgccgccgt
    56701 cagcattgat gttcgtaata tgccggagtc accagaaata ttcgaacagg cgatgagtaa
    56761 cctgcctgac gctttctcac cgcaactact gttcctggat gccgaccgta ataccttaat
    56821 tcgtcgttac agtgacacgc gccgactgca tccgctttcc agcaaaaacc tgtcgctgga
    56881 aagtgctatc gacaaagaaa gcgatttgct ggagcctctg cgttcgcgag cggatctgat
    56941 tgtcgatacc tcagaaatgt ccgttcacga gctggctgaa atgctgcgta cccgtctgct
    57001 gggtaaacgt gaacgcgaac tgaccatggt ctttgagtct ttcggcttca aacacggtat
    57061 ccctatcgat gcagattacg tctttgacgt gcgcttcttg ccgaacccgc actgggatcc
    57121 gaaactgcgt ccaatgacag gtcttgataa acctgtcgcc gcgttcctcg accgccacac
    57181 agaagtacac aattttatct accagacgcg aagctatctt gagctatggt tacctatgct
    57241 ggaaaccaac aaccgtagct acctgacggt cgccattggt tgtaccggcg ggaagcaccg
    57301 ttcggtgtat attgcagagc aactggcaga ctacttccgc tcgcgcggta aaaacgtcca
    57361 gtcacgccat cgtacgctgg aaaaacgtaa accatgaccg tcaagcaaac tgttgaaatc
    57421 acaaacaagc tgggcatgca tgcccggcct gcaatgaagc tgtttgaatt aatgcagggt
    57481 tttgacgctg aagtgctgtt acgtaatgac gaaggcaccg aggctgaagc caacagcgtt
    57541 attgcgctgc tgatgttgga ttctgccaaa ggacggcaga ttgaagttga agcgaccggt
    57601 ccacaggaag aggaagcact ggccgccgtt atcgccctct ttaattctgg ttttgatgaa
    57661 gattaatctt catcactttg acatacaact cccttcaagc tcccccgaca ataagaaaat
    57721 cacgtactga aatcgttctc aatcaacgtc atttgtacat tttgtgcgct tttcactttc
    57781 agaagaacct taagaaaacc ttaagaggca ttgtttaggt tttgtttaag ttaatcgacc
    57841 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    57901 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    57961 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    58021 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    58081 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc gattttgacg
    58141 gcaagccttc cattacgctg accgaatttg ctgaacaatg ccgttacgaa gaagacattg
    58201 cacaactgcg ccagctactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    58261 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttatcc
    58321 cggtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    58381 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    58441 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    58501 gcgaagctta atccagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    58561 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgaggag actttgaaac
    58621 gaagtggatt aggtaataca gctgccagta atgcagcttc cgaccgggta agtttgctcg
    58681 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    58741 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    58801 gcgttaatcc agcttccagc ccttttcgta cccagctacg cccatcccat aaaaagagat
    58861 tttggccgtc tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt
    58921 gtgcgccagg gctttctcaa tggaagcgac atcaaagccc cagtgctcag gaaatttctg
    58981 atcttctgcg gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca
    59041 gtcagaatgt gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac
    59101 cattaccgct gagaagggaa caggcgcaac gctaaacaac gcgatgcccc cgccccagaa
    59161 aacggcgagg acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa
    59221 gcggctttta ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc
    59281 gccgcttctg caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct
    59341 tcatcaacca cgatatcatc gacaggacac ggcacatgct ccgcgcccat ctcttccagc
    59401 acttctgcgg tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc
    59461 ggcagcatcg ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct
    59521 tgtgccagcg cctttaattc acggtcaacg gtgcattcgc taccaagact ggcaaaattg
    59581 cttaaattct tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca
    59641 tcggcctggg ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcaca
    59701 ttgcgcgttt ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta
    59761 tccggtgcaa agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc
    59821 gcttcatgaa tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc
    59881 ttcattgtga tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg
    59941 tacgcatcac acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact
    60001 gcgtgcggcc tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca
    60061 gccaagattt ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt
    60121 tttagtggct tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt
    60181 catctcttca atccattcac cgacgttatc ttcccaggcg ggaaggtcag gagactgaat
    60241 ttgctgaccc agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt
    60301 atgtccttcc tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct
    60361 gacatagccc ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc
    60421 tacaagttcg agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt
    60481 ctcttctgtc gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat
    60541 cgcggttagc gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag
    60601 gtactcttgt ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc
    60661 gcgcggataa cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg
    60721 aatatccagc aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc
    60781 ggtcatggcg acatcaacgc tgttacctaa tttttccagc acagaacgcg cgacaatcac
    60841 gttcagttca atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc
    60901 atcaaacgca tcatcgacct cttctgccac cgacggtgcg tggatcgtca acgtaaaggt
    60961 tgaacctttg ccctgttcac tggtaaccgt aatatcgccg cccatatttt tcgccagacg
    61021 acgagaaacg gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt
    61081 cacctggtaa tacatggcga agattttatc cagctcatcc tgcggaatgc cgataccaga
    61141 atcttccact tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg
    61201 gccttgctgg gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg
    61261 cgtcccgtcg gtaatgacct gatgcggtaa tggcagcgta ggctccaggt taaagcgcaa
    61321 tcctttttgt tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa
    61381 atcaaccggc tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat
    61441 atcgttaaag atattcccca gcgtgacggc cgaaacatgg atagttttga gatatttttc
    61501 ctgctcggcg gtgagttcgg tatccagcag aatgcggctc aggccgacga taccgttcag
    61561 cggtgtacgc aattcgtgac tgatggtgga aataaacgtc gttttgtcgc ggctggcgcg
    61621 ttcaagcgca tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc
    61681 gtgacgttta cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg
    61741 cccgtccggg taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt
    61801 ttcatcggtt tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag
    61861 gtgaaccagt tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga
    61921 aaactcttta tcttcgttac gataaaaaac caagtcgggt gaagcatcaa ggaaggaacg
    61981 taagaatgag gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat
    62041 tttcagttgg ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc
    62101 ctgatttagc tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg
    62161 catctcctcc agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc
    62221 gacgaccacc gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga
    62281 acgaataaca tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac
    62341 cacaatggca agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa
    62401 cttcatcatc aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt
    62461 ccttcacgac aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg
    62521 agaaatgcgg aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc
    62581 gccgatccct gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat
    62641 cgattttcgc accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg
    62701 tggtagatca cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtataatcc
    62761 tccagttcaa taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc
    62821 acaatatgca gcggatgcag ctttatgccg tccacgctgg tttcaaccac tcgctccagc
    62881 gtttgcaaac attcagcctg cccttcgcca ggcaggccga caattaagtg ggaacacacc
    62941 ttcagcccgc gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg
    63001 cggttgatgc gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact
    63061 tcgtaaccct ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc
    63121 gggcgggtac caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata
    63181 gaacgcagca cctgaacttc cgcaaaggtg ctggtatacg cctgaaagta ggccagatag
    63241 cgtttagcgc ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc
    63301 tgctgcgctt catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg
    63361 gtaccgtcac ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc
    63421 ccataacgac gggtgagatc accaccaaac atattgacta atttctgtaa ctgcataatc
    63481 tgatagaccg cgccttgaaa agaggccaaa gcctgccatt tttagctcaa ttcggcgatg
    63541 acctggatca atcgtcccgg cctgctttta tcaactgcat aatcaatcaa aattaccgaa
    63601 atttcatgca caatcacata aatcactttt gcttatcttg tgtcagattt ttttatctcc
    63661 ttatggattt taggcaaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa
    63721 atgctggttt tctcgtcagt tcaaggcagg ataagggtta acacgccttt atgacagtca
    63781 ggaattgact gtttctctaa cgactctcct tttagcctta aagataaaat ctattttaat
    63841 ttcagctatt taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca
    63901 tttgacctgt atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc
    63961 agcctgctca tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc
    64021 gaaaaggaca acaagggggc gaatgcgagg cgcgcgtatg acacgcaaac cccgtcgcca
    64081 cgctctttct gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga gcctggggga
    64141 ggttcacgat atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat
    64201 cgcccacata gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc
    64261 ccgcatgcag caccgtggcg cgattctcgc cgatggtaaa accggcgacg gttgcggctt
    64321 gctgttacaa aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt
    64381 agcaaaaaac tacgctgtcg ggatgctctt cctgaataaa gatcctgaac tcgccgctgc
    64441 cgcacgccgc atcgttgaag aagaactgca acgcgaaacc ttgtcgattg tgggctggcg
    64501 tgatgtcccc actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc tgccacgcat
    64561 tgagcaaatt tttgtgaacg ccccggcagg ctggcgtcca cgcgatatgg agcgccgtct
    64621 gtttatcgcc cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg
    64681 tagcctgtcg aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg
    64741 cttttatctg gatcttgcgg acctgcgtct ggaatcggcc atttgcctgt tccaccagcg
    64801 cttctccact aacaccgtac cgcgctggcc gctggcgcaa ccgttccgct atctggcgca
    64861 taacggtgaa atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa
    64921 attccagacg ccgctgatcc ctgacctgca cgacgccgcg ccattcgtca acgaaaccgg
    64981 ttctgactcc agttccatgg ataacatgct ggaactgctg ctggcaggcg ggatggatat
    65041 catccgcgcc atgcgcctgt tagtgccacc cgcctggcag aacaacccgg atatggaccc
    65101 ggaactgcgt gccttctttg actttaactc catgcatatg gagccgtggg atggtccggc
    65161 gggcatcgtg atgtccgatg gtcgcttcgc tgcctgtaac ctcgaccgta acggtctgcg
    65221 tccggcacgc tacgtcatta ccaaagacaa gctcatcacc tgcgcctctg aagtcggtat
    65281 ctgggattac cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat
    65341 ggtgatcgac actcgcagtg ggcgtattct gcactcggca gaaaccgatg acgatctgaa
    65401 aagccgccac ccttataaag agtggatgga gaaaaacgtc cgccgactgg taccgtttga
    65461 agatctgccc gatgaagaag tgggtagccg cgaactggac gacgacacgc ttgccagcta
    65521 ccagaaacag tttaactaca gcgcggaaga gctggactcc gtaattcgcg tactgggcga
    65581 aaacggtcag gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag
    65641 tcagccgcgc attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc
    65701 aatcgacccg ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat
    65761 gaacgtcttt tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct
    65821 gctctactcc gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac
    65881 gctggatatc acctttgacg tcactaaaac cacgctcgaa gcgacagtca aagagctatg
    65941 cgacaaagcc gaaaaaatgg tacgtagcgg caccgtgttg ctggtgctct ccgaccggaa
    66001 tatcgctaaa gatcgcctgc cggttccggc tccgatggcg gttggcgcga tccagacccg
    66061 tctggtcgat caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg
    66121 cgatccgcac cacttcgccg tgttgctggg tttcggcgcg acggctattt atccgtacct
    66181 tgcctatgaa acgctgggcc gcctggtaga cacccacgcg attgccaaag attatcgtac
    66241 cgtgatgctc aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat
    66301 gggcatctcc accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctaca
    66361 cgatgatgta gtgggcctgt gcttccaggg cgcggtcagc cgcattggtg gggcaagctt
    66421 tgaagacttc cagcaggatc tgctgaatct gtcgaaacgt gcctggctgg cgcgcaagcc
    66481 gattagccag ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cctacaaccc
    66541 ggacgtggtg cgcacgctgc aacaggcggt acaaagcggc gagtacagcg actatcagga
    66601 atacgcgaag ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac
    66661 gccgggtgaa aacgcggtca acattgctga tgttgaaccg gcaagcgaac tgtttaaacg
    66721 ctttgatacc gccgcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc
    66781 ggaagcgatg aatagcatcg gcggtaactc gaactccggt gaaggcggcg aagacccggc
    66841 gcgctatggc actaacaaag tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt
    66901 tactccggcg tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa
    66961 gccgggcgaa ggtggtcagt tgccgggcga caaagtcact ccttacatcg ccaaactgcg
    67021 ctattcggtg cccggagtga cgctgatctc cccgccgccg caccacgata tctactctat
    67081 cgaagactta gcgcagctta ttttcgacct caagcaggtt aacccgaaag cgatgatctc
    67141 cgttaagctg gtgtccgaac caggcgttgg caccatcgcg actggcgtgg cgaaagctta
    67201 tgcggacttg atcaccatcg cgggctatga cggcggcacc ggcgcaagcc cgctgtcgtc
    67261 agtgaaatat gcaggctgcc cgtgggaact cggtctggtg gaaacccaac aggcgctggt
    67321 tgccaacggc ttgcgtcata aaattcgttt gcaggttgat ggcggcctga aaacgggtgt
    67381 cgatatcatc aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat
    67441 ggtggcgctc ggctgtaaat atctgcgtat ttgccacctg aacaactgcg ccacgggtgt
    67501 agcaactcag gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac
    67561 taattacttt gagtttatcg cccgtgaaac ccgcgagctg atggcacagc tgggagtaac
    67621 gcgtctggtg gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc
    67681 caaacagcag aaactggcgc tgtcgaagct gctggagact gccgagccac atgcaggtaa
    67741 gtcactctac tgcactgaaa acaacccgcc gtttgataac ggcctactga acgcgcagtt
    67801 gctgcaacag gcgaaaccgt ttgtcgatga acgccagagc aaaaccttct ggttcgatat
    67861 ccgcaacacc gaccgttctg tcggcgcgtc gctttcaggc tatatcgccc agacgcacgg
    67921 cgatcagggg ctggcagccg atcctatcaa agcgtacttc aacggcaccg caggccagag
    67981 tttcggtgtg tggaacgcgg gcggcgtgga actgtacctg accggggatg ccaacgacta
    68041 tgtcggtaaa ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt
    68101 ccgcagccat gaagcaagca ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg
    68161 tctgtatgcc gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg gtgctatcac
    68221 cgtggtagaa ggcattggcg acaacggctg tgaatatatg acgggcggta ttgtctgtat
    68281 tctgggtaaa accggcgtta acttcggcgc gggcatgacc ggcggcttcg cttacgttct
    68341 cgatgaaagc ggtgatttcc gcaaacgcgt taacccggaa ctggtcgaag tcttaagcgt
    68401 cgacgatctg gcgatccatg aagagcatct gcgcggtctt atcaccgagc atgttcagca
    68461 taccggttct cagcgcggtg aagagattct ggcgaactgg tcaaccttcg ccacgaaatt
    68521 cgcgctggtt aaaccgaagt ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag
    68581 cgcagcagag ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta
    68641 tcaatttatc gacctgcagc gcgttgatcc gccaaagaaa ccgctgaaga tccgtaaaat
    68701 tgagtttgtt gaaatttacg agccgttttc cgaaggccag gccaaagcgc aggctgaccg
    68761 ctgtctgtcg tgcggcaacc catactgcga gtggaaatgc ccggtacaca actacatccc
    68821 aaactggctg aagctcgcca acgaggggcg tatttttgaa gcggcagaac tgtcgcatca
    68881 gaccaacaca ctgccggaag tatgcggacg tgtctgcccg caagaccgtc tgtgcgaagg
    68941 ttcctgcacc ctgaacgatg agttcggtgc ggtgaccatc ggcaacattg agcgttatat
    69001 caatgataaa gcgttcgaaa tgggctggcg tccggatatg tccggcgtga agcaaaccgg
    69061 taaaaaagtg gcgattatcg gtgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac
    69121 gcgtaacggc gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac
    69181 cttcggtatt ccggccttca agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt
    69241 caccggcatg ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tacagctgga
    69301 cgatctgctg agtgattacg atgccgtgtt ccttggcgtt gggacttatc agtcaatgcg
    69361 cggcgggctg gaaaacgaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc
    69421 caacaccaaa cagttaatgg gctttggtga aacccgcgac gaaccgttcg tcagcatgga
    69481 aggcaaacgc gtggtagtac ttggcggtgg cgacactgcg atggactgcg tgcgcacgtc
    69541 tgtacgccag ggggcaaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc
    69601 gggttcccgc cgcgaagtga aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt
    69661 ccagccactg gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg
    69721 taccgaaatg ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc
    69781 cgaacatatc gttccggcag atgcggtgat catggcgttt ggtttccgtc cacacaacat
    69841 ggaatggctg gcaaaacaca gcgtcgagct ggattcacaa ggccgcatca tcgctccgga
    69901 aggcaacgac aacgctttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt
    69961 ccgtggttcc gatctggtcg ttaccgccat cgccgaaggt cgcaaagcgg cagacggcat
    70021 tatgaactgg ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc
    70081 gctgaaattc tcctttcttt ctggaaatcg cctcgcataa tctgaagaaa accaatgtaa
    70141 tacgctcatc tggattgtta ccggtagatg cccggcgtat agctgattcc attattcaac
    70201 gcagcgccat ataaagggac agataatgga atcgctctca gagagaacct caacaggcta
    70261 ccagcaaatc cacaacggca ttattcatct ggtcgatagc gcccgaacgg aaacggtacg
    70321 tagcgttaac gcgctgatga ccgcgacgta ctgggaaatt ggccgacgga ttgtcgaatt
    70381 tgaacaaggt ggtgaggcca gagcagctta tggtgcacag ttgattgatc ggctatcgaa
    70441 ggatttaagt cgacggtata aacgtggggt ctctgcaaaa aacttacgcc aaatgaggct
    70501 tttttacctc ttttttcaac atgttgaaat tcgccagaca gtgtctggcg aattaacacc
    70561 attgggaatt ccgcagacac catctgcaga attcccatcg gcaaagattt ggcagacgct
    70621 gtctgccaaa ttgagaggct gtttgatatt tccacagctt ttgtattgca tctcctattt
    70681 tcggtttgta tattcatgca aataaacgaa ggaaatgagg tagcctgagt ttaacggaca
    70741 ctccttcctg aaatagaatg gcatcagaag gagctaataa tgagcagaaa aacccaacgt
    70801 tactctaaag agttcaaagc cgaagctgtc agaacggttc ttgaaaatca actttcgatc
    70861 agtgaaggcg cttcccgatt atctcttcct gaaggcactt taggacaatg ggttaccgca
    70921 cggtggctga actggaatct gaaattctgc aactgcgtaa ggcgttaaat gaagctcgcc
    70981 ttgagcgaga tatat
//
LOCUS       DAETQL010000007        67800 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.007, whole genome shotgun sequence.
ACCESSION   DAETQL010000007 DAETQL010000000
VERSION     DAETQL010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.007"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..111
                     /locus_tag="K0W65_000492"
     CDS             <1..111
                     /locus_tag="K0W65_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3093890.1"
                     /translation="LRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            127..357
                     /locus_tag="K0W65_000493"
                     /pseudo
     CDS             127..357
                     /locus_tag="K0W65_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
     gene            752..1391
                     /locus_tag="K0W65_000494"
                     /pseudo
     CDS             752..1391
                     /locus_tag="K0W65_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1494..1817
                     /locus_tag="K0W65_000495"
     CDS             1494..1817
                     /locus_tag="K0W65_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HBI3093891.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1884..3341)
                     /gene="nuoN"
                     /locus_tag="K0W65_000496"
     CDS             complement(1884..3341)
                     /gene="nuoN"
                     /locus_tag="K0W65_000496"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBI3093892.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3348..4877)
                     /gene="nuoM"
                     /locus_tag="K0W65_000497"
     CDS             complement(3348..4877)
                     /gene="nuoM"
                     /locus_tag="K0W65_000497"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBI3093893.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(5041..6882)
                     /gene="nuoL"
                     /locus_tag="K0W65_000498"
     CDS             complement(5041..6882)
                     /gene="nuoL"
                     /locus_tag="K0W65_000498"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBI3093894.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6879..7181)
                     /gene="nuoK"
                     /locus_tag="K0W65_000499"
     CDS             complement(6879..7181)
                     /gene="nuoK"
                     /locus_tag="K0W65_000499"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBI3093895.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7178..7732)
                     /gene="nuoJ"
                     /locus_tag="K0W65_000500"
     CDS             complement(7178..7732)
                     /gene="nuoJ"
                     /locus_tag="K0W65_000500"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBI3093896.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7744..8286)
                     /gene="nuoI"
                     /locus_tag="K0W65_000501"
     CDS             complement(7744..8286)
                     /gene="nuoI"
                     /locus_tag="K0W65_000501"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBI3093897.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8301..9278)
                     /gene="nuoH"
                     /locus_tag="K0W65_000502"
     CDS             complement(8301..9278)
                     /gene="nuoH"
                     /locus_tag="K0W65_000502"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBI3093898.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9275..12001)
                     /gene="nuoG"
                     /locus_tag="K0W65_000503"
     CDS             complement(9275..12001)
                     /gene="nuoG"
                     /locus_tag="K0W65_000503"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBI3093899.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(12054..13391)
                     /gene="nuoF"
                     /locus_tag="K0W65_000504"
     CDS             complement(12054..13391)
                     /gene="nuoF"
                     /locus_tag="K0W65_000504"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBI3093900.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13388..13888)
                     /gene="nuoE"
                     /locus_tag="K0W65_000505"
     CDS             complement(13388..13888)
                     /gene="nuoE"
                     /locus_tag="K0W65_000505"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBI3093901.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13891..15693)
                     /gene="nuoC"
                     /locus_tag="K0W65_000506"
     CDS             complement(13891..15693)
                     /gene="nuoC"
                     /locus_tag="K0W65_000506"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBI3093902.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15787..16449)
                     /gene="nuoB"
                     /locus_tag="K0W65_000507"
     CDS             complement(15787..16449)
                     /gene="nuoB"
                     /locus_tag="K0W65_000507"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HBI3093903.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16465..16908)
                     /gene="nuoA"
                     /locus_tag="K0W65_000508"
     CDS             complement(16465..16908)
                     /gene="nuoA"
                     /locus_tag="K0W65_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBI3093904.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17539..18477)
                     /gene="lrhA"
                     /locus_tag="K0W65_000509"
     CDS             complement(17539..18477)
                     /gene="lrhA"
                     /locus_tag="K0W65_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBI3093905.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19396..20613
                     /gene="alaA"
                     /locus_tag="K0W65_000510"
     CDS             19396..20613
                     /gene="alaA"
                     /locus_tag="K0W65_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HBI3093906.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20697..21296
                     /gene="yfbR"
                     /locus_tag="K0W65_000511"
     CDS             20697..21296
                     /gene="yfbR"
                     /locus_tag="K0W65_000511"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBI3093907.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLMKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21355..23187)
                     /locus_tag="K0W65_000512"
     CDS             complement(21355..23187)
                     /locus_tag="K0W65_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBI3093908.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILTVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23274..23924)
                     /gene="hxpA"
                     /locus_tag="K0W65_000513"
     CDS             complement(23274..23924)
                     /gene="hxpA"
                     /locus_tag="K0W65_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HBI3093909.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEADLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23935..24429)
                     /locus_tag="K0W65_000514"
     CDS             complement(23935..24429)
                     /locus_tag="K0W65_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBI3093910.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24512..24967)
                     /locus_tag="K0W65_000515"
     CDS             complement(24512..24967)
                     /locus_tag="K0W65_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBI3093911.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25305..26507
                     /gene="ackA"
                     /locus_tag="K0W65_000516"
     CDS             25305..26507
                     /gene="ackA"
                     /locus_tag="K0W65_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBI3093912.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26582..28726
                     /gene="pta"
                     /locus_tag="K0W65_000517"
     CDS             26582..28726
                     /gene="pta"
                     /locus_tag="K0W65_000517"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBI3093913.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28916..30436
                     /gene="yfcC"
                     /locus_tag="K0W65_000518"
     CDS             28916..30436
                     /gene="yfcC"
                     /locus_tag="K0W65_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBI3093914.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30469..31011)
                     /gene="yfcD"
                     /locus_tag="K0W65_000519"
     CDS             complement(30469..31011)
                     /gene="yfcD"
                     /locus_tag="K0W65_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBI3093915.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(31069..31620)
                     /gene="yfcE"
                     /locus_tag="K0W65_000520"
     CDS             complement(31069..31620)
                     /gene="yfcE"
                     /locus_tag="K0W65_000520"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBI3093916.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31676..32320)
                     /gene="yfcF"
                     /locus_tag="K0W65_000521"
     CDS             complement(31676..32320)
                     /gene="yfcF"
                     /locus_tag="K0W65_000521"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBI3093917.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32456..33103
                     /gene="yfcG"
                     /locus_tag="K0W65_000522"
     CDS             32456..33103
                     /gene="yfcG"
                     /locus_tag="K0W65_000522"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBI3093918.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33160..33522
                     /gene="folX"
                     /locus_tag="K0W65_000523"
     CDS             33160..33522
                     /gene="folX"
                     /locus_tag="K0W65_000523"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBI3093919.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33543..34436
                     /locus_tag="K0W65_000524"
     CDS             33543..34436
                     /locus_tag="K0W65_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HBI3093920.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34484..35374)
                     /gene="rpnB"
                     /locus_tag="K0W65_000525"
     CDS             complement(34484..35374)
                     /gene="rpnB"
                     /locus_tag="K0W65_000525"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HBI3093921.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35571..36344)
                     /gene="hisP"
                     /locus_tag="K0W65_000526"
     CDS             complement(35571..36344)
                     /gene="hisP"
                     /locus_tag="K0W65_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBI3093922.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36352..37068)
                     /locus_tag="K0W65_000527"
     CDS             complement(36352..37068)
                     /locus_tag="K0W65_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3093923.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(37065..37751)
                     /gene="hisQ"
                     /locus_tag="K0W65_000528"
     CDS             complement(37065..37751)
                     /gene="hisQ"
                     /locus_tag="K0W65_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HBI3093924.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37841..38623)
                     /gene="hisJ"
                     /locus_tag="K0W65_000529"
     CDS             complement(37841..38623)
                     /gene="hisJ"
                     /locus_tag="K0W65_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBI3093925.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38844..39626)
                     /gene="argT"
                     /locus_tag="K0W65_000530"
     CDS             complement(38844..39626)
                     /gene="argT"
                     /locus_tag="K0W65_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBI3093926.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39892..40461)
                     /gene="ubiX"
                     /locus_tag="K0W65_000531"
     CDS             complement(39892..40461)
                     /gene="ubiX"
                     /locus_tag="K0W65_000531"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HBI3093927.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40556..42073)
                     /gene="purF"
                     /locus_tag="K0W65_000532"
     CDS             complement(40556..42073)
                     /gene="purF"
                     /locus_tag="K0W65_000532"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBI3093928.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(42110..42598)
                     /gene="cvpA"
                     /locus_tag="K0W65_000533"
     CDS             complement(42110..42598)
                     /gene="cvpA"
                     /locus_tag="K0W65_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBI3093929.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42857..43519)
                     /gene="dedD"
                     /locus_tag="K0W65_000534"
     CDS             complement(42857..43519)
                     /gene="dedD"
                     /locus_tag="K0W65_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBI3093930.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43509..44777)
                     /gene="folC"
                     /locus_tag="K0W65_000535"
     CDS             complement(43509..44777)
                     /gene="folC"
                     /locus_tag="K0W65_000535"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBI3093931.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44847..45761)
                     /gene="accD"
                     /locus_tag="K0W65_000536"
     CDS             complement(44847..45761)
                     /gene="accD"
                     /locus_tag="K0W65_000536"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBI3093932.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45917..46576)
                     /locus_tag="K0W65_000537"
     CDS             complement(45917..46576)
                     /locus_tag="K0W65_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI3093933.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46659..47471)
                     /gene="truA"
                     /locus_tag="K0W65_000538"
     CDS             complement(46659..47471)
                     /gene="truA"
                     /locus_tag="K0W65_000538"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBI3093934.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47471..48484)
                     /locus_tag="K0W65_000539"
     CDS             complement(47471..48484)
                     /locus_tag="K0W65_000539"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBI3093935.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48550..49686)
                     /gene="pdxB"
                     /locus_tag="K0W65_000540"
     CDS             complement(48550..49686)
                     /gene="pdxB"
                     /locus_tag="K0W65_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBI3093936.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49785..50780
                     /gene="flk"
                     /locus_tag="K0W65_000541"
     CDS             49785..50780
                     /gene="flk"
                     /locus_tag="K0W65_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBI3093937.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50777..51955)
                     /locus_tag="K0W65_000542"
     CDS             complement(50777..51955)
                     /locus_tag="K0W65_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3093938.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52210..53451)
                     /gene="fabB"
                     /locus_tag="K0W65_000543"
     CDS             complement(52210..53451)
                     /gene="fabB"
                     /locus_tag="K0W65_000543"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBI3093939.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53610..55616
                     /gene="mnmC"
                     /locus_tag="K0W65_000544"
     CDS             53610..55616
                     /gene="mnmC"
                     /locus_tag="K0W65_000544"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBI3093940.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55737..56015)
                     /locus_tag="K0W65_000545"
     CDS             complement(55737..56015)
                     /locus_tag="K0W65_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBI3093941.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(56049..56597)
                     /gene="epmC"
                     /locus_tag="K0W65_000546"
     CDS             complement(56049..56597)
                     /gene="epmC"
                     /locus_tag="K0W65_000546"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBI3093942.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56597..57406)
                     /locus_tag="K0W65_000547"
     CDS             complement(56597..57406)
                     /locus_tag="K0W65_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBI3093943.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57406..58230)
                     /gene="mepA"
                     /locus_tag="K0W65_000548"
     CDS             complement(57406..58230)
                     /gene="mepA"
                     /locus_tag="K0W65_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBI3093944.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58234..59319)
                     /gene="aroC"
                     /locus_tag="K0W65_000549"
     CDS             complement(58234..59319)
                     /gene="aroC"
                     /locus_tag="K0W65_000549"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBI3093945.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59354..60286)
                     /gene="prmB"
                     /locus_tag="K0W65_000550"
     CDS             complement(59354..60286)
                     /gene="prmB"
                     /locus_tag="K0W65_000550"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBI3093946.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60452..61003
                     /gene="smrB"
                     /locus_tag="K0W65_000551"
     CDS             60452..61003
                     /gene="smrB"
                     /locus_tag="K0W65_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBI3093947.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(61107..61997)
                     /locus_tag="K0W65_000552"
     CDS             complement(61107..61997)
                     /locus_tag="K0W65_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBI3093948.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61984..62508)
                     /locus_tag="K0W65_000553"
     CDS             complement(61984..62508)
                     /locus_tag="K0W65_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3093949.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62505..62975)
                     /locus_tag="K0W65_000554"
     CDS             complement(62505..62975)
                     /locus_tag="K0W65_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3093950.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62972..63520)
                     /locus_tag="K0W65_000555"
     CDS             complement(62972..63520)
                     /locus_tag="K0W65_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3093951.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63525..64247)
                     /locus_tag="K0W65_000556"
                     /pseudo
     CDS             complement(63525..64247)
                     /locus_tag="K0W65_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64267..64989)
                     /locus_tag="K0W65_000557"
     CDS             complement(64267..64989)
                     /locus_tag="K0W65_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBI3093952.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(65049..66203)
                     /gene="yfcU"
                     /locus_tag="K0W65_000558"
                     /pseudo
     CDS             complement(65049..66203)
                     /gene="yfcU"
                     /locus_tag="K0W65_000558"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66152..66907)
                     /locus_tag="K0W65_000559"
     CDS             complement(66152..66907)
                     /locus_tag="K0W65_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBI3093953.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66989..67552)
                     /locus_tag="K0W65_000560"
     CDS             complement(66989..67552)
                     /locus_tag="K0W65_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI3093954.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16961 a        18310 c        16819 g        15710 t
ORIGIN      
        1 ctgagacaac atctggcaag gctgggacgg aagtcactgt cgttctcaaa atcggtggag
       61 ctgcatgaca aggtcatcgg gcattatctg aacataaaac actatcagta agttggagtc
      121 attaccaggg aaagcaattc tgacagcgcc ccctatacgt tattaaaaaa ctatgctgac
      181 agatttaaaa aatattcagc agaagagcag tacgaaattg attttcaaca tcgactggta
      241 aacagaaatt gttatttaga taaatatggc gatgcacgaa tcaatgcttc ttatacacaa
      301 ttagaaataa aacactcaca acccaaaaat agagcatccg gcaaaagagt gagttaacat
      361 cattgttaat caattcgacc agtcagtaaa tatgctgact ggtcgaacat aatttgctaa
      421 aaagtcatca ctgactgatg tcagtcccac catctgccag ttcaatctac gggtatttcc
      481 agcatatcgc ggcacgcgat tgctggtctg ttgatgtagt ggattgtagt tgctgacttt
      541 tcttaccctc agatttctgc cccacttgtc aattcaatct gccaataaat aacataatta
      601 tatttttatt cctcacagaa attccttttt aaaaatagcg aacccactgt aaaaaacact
      661 ttttgtatac ctctaccaag cgtaataaaa taaacgctca taattcatta taaataataa
      721 tgaaataacg ccttttattg ataatttaca tatgaaaaaa ttttcctttt gttatattat
      781 ttctgttttc actccctctg attattttta ccaaccctgg gtcactgcat tatcgccaac
      841 gccccgacac gtaagtcctg agcaattaga aaaaacagtt cgttatctta cacaaactgt
      901 gcatccacgt aatgccggca atattgataa tctgaatagg tccgcagagt atataaaaga
      961 gttctttgtc agtagcggtg ccagagttac cgcgcagggc gtccccgatt ggctattacg
     1021 actctgcgcc tggaagccag aattaccctt acccggcaat gtcctggctt tatcccgatc
     1081 ggggagactt tattgctgtg gtcggcagaa tgcaggatat caacgccgtt cgtcaggtaa
     1141 aagcggcatt gttgtcatcc cgggatttat ctgtttattc tatgaatgcc ccagggttta
     1201 ttcccggtat tgatttttct gatcacctca attattggca acacgatatt cccgccgtaa
     1261 tgattactga caccgctttt tatcgtaata aacaatacca cttgcccggt gataccgcag
     1321 acagattgaa ttatcagaaa atggctcagg tggtagatgg tgttataacc ttgttataca
     1381 acagtaaata ataaaaattt ctcgaggcgg gtccagaaac agcgacatgt acattttatt
     1441 ttccctcgtc tgttttaaga tgcgaaacat aaaattcaat ggaggaaaaa taaatgggaa
     1501 tgattggcta ttttgcagaa attgactcag aaaagattaa ccaactgctt gagtctactg
     1561 aaaaaccttt gatggataat atacatgaca cgctttcagg attacgccga ctggaatcac
     1621 tggatcgaag tgagctgcgt aaacagtttt ccattaagcg actcaatgaa atggagattt
     1681 atccgggagt gaccttttca gaagagttag aggggcaact ctttgcctcg atcatgcttg
     1741 atatggaaaa gctgattagc gcgtatcgac gtatgctgcg acaagggaat catgcgctga
     1801 cggttattgt tgggtgatgg tgtcagagcg ccaaaaaaaa ccgccgaaca tgtcggcggc
     1861 tttctgactt acaaactaac agtttacatc agcggcattg ctaaccgcac aatactaatc
     1921 agcggttgtg gccatacacc cagcaccagt accaacagtg cagaaatcag caccacgata
     1981 ccgcccgcgc tgtactgcca gtttgacggt gcatcgcgac ccggttgttc cggtgcgtgc
     2041 aggtacaggc tcaccgccac gcgcaggtag tagtagaggc cgattgccga accgacaacc
     2101 acggcaccca ccagccacca caagtgtgcc tggacaccga ccgccagcac gtagaactta
     2161 ccgataaagc ccagcgtcat cgggataccg gccagagaca gcatcatcac cgtcatcact
     2221 gccgcgagga tcggacgatg ccagaacaga ccgcggtaag agaacaggga atcagcatcc
     2281 gggccacgat acgggctgga catcaggctg accacgccga acgcgccgag gctgctgaac
     2341 agataaccgg ccaggtaaac ccctaccgct tccatcgaca tctcgccggt ttgcagcgca
     2401 atcagcgcca ccagcagata gccgaggtga gagatagatg agtaaccgag cagacgtttg
     2461 atgttggtct ggctcaacgc catcaggtta ccgaagatga tggaggcaaa ggcgataatc
     2521 gccagtacta cgcgaatcgc ttcgctgtca cccaccggtg cgtagaggaa cagacgcatc
     2581 accacaccga agatagcgat tttgctcgcc gtcgccagga aagtggaaac cggcgcaggc
     2641 gcgccctggt atacgtctgg cgtccacagg tggaacggca ccagagagag tttgaagccg
     2701 aggccaacaa tcatcatgcc gaaacctgcc agcaacagcg gctcgttgag cataccgtcg
     2761 ccgaggtttt tacccagcgc gacaaacgac aggtcgccag actgcgcata caccagcgcc
     2821 ataccgaaca gcaggaaaga agacgctgcg gcagaaagga tggtgtattt gatactggct
     2881 tccagcgaac gtttctggcg gaaagcgtaa ccgaccaggc caaacagcgg caaagagatc
     2941 agttcaatac cgaggaacag agacgccaga tggtttgcat tcgccagcag gatcccgccc
     3001 agcgcggcaa ttaacaccaa caggtagaac tcatccttgt tgtcgttata gccttcaagc
     3061 cacgggtagg cgaaagtaca ggtggcgagg ctcgccaaca ataccagccc ggtgtaaagc
     3121 atggcgaaac catcaacgcg catcagcggc gtaacgtcca tagcgcccgc ctggccaaca
     3181 aaccagagcg aaaccagcgc cgcgttaagc ccaataaccg agagcgtagc gttgaggaaa
     3241 tgattgcgtc gccacgcaat ggagagcatc acaaccacca ccgtcaagcc gacgatcagc
     3301 aacggtagca gtgcgatcag gttttgtgga gttattgtca tggcgattta cggccttgta
     3361 gtagtaacgg aattaacaaa ccactgctgg atattgccaa tcgcggagtg cgaggtatcc
     3421 agaatcggct gcggatagaa gcccagcagt accagcagca ccaccagcaa caggatcata
     3481 aacagctcac gcagcgacat ccctggcagt tcctggctgg caatctggct tttcgcttta
     3541 ccgaagtaag cgcgatgcaa catcgccagc gaataaacag atgcaaagac cagcccaaag
     3601 gtagagataa cggtaatcac cgggacaacc tggaagctgc cgaacagaat cataaattcg
     3661 ccgacgaagt taccggtgcc aggcatccca agcgttgcca ccgcaaagaa cagcgacagt
     3721 gctggcagcc atttcatctt gctccacaga ccgcccatca tgcgcatgtc gcgggtatgg
     3781 atacgttcat aaagctgacc gcagagaata aacagacccg ccgccgacaa gccgtgcgca
     3841 atcatctgga ttaccgcgcc ctggtaggcc aactggctgc cggtgtagat tgcaatcagc
     3901 acgaagccca tgtgggaaac cgaggtgtag gcgatcagac gtttgatatc ggtctgagcg
     3961 aaggtcatcc acgcaccgta gaagatgccg ataacaccca gccacatagc gattggcgcg
     4021 aactctgccg acgcgttcgg gaacagcggc agggagaaac gcaacaaacc gtaagcggcg
     4081 gttttcagca agatccccgc gaggtccacg gaaccggcgg tcggagcctg ggagtgcgca
     4141 tccggcagcc agccatgcag cggaaccacc ggcattttga ctgcgaaggc gatgaagaag
     4201 cccagcatca acagatattc cacaccactg gacattggcg tattcagcag ctcttcatag
     4261 ttgaaggtcc agacgccggt cgcattgtag tgaacaaaaa ccagcgccag gatggcgatc
     4321 aacatcacca gaccactcgc ctgggtgtaa atgaagaact tggttgccgc cgtgatacgc
     4381 gttttaccgt cagaggcttt atgcccccac agtgcgatca ggaagtacat cggcaccagc
     4441 atcatttccc agaagaagaa gaacaggaac atgtcgatgg caaggaacac gccgataacg
     4501 ccgcccagga tccacatcag gttgaggtgg aagaagccct gatatttttc gatctctttc
     4561 cacgaacata gtaccgccag cacaccgagc agaccggtca gcacgaccat cagcagcgac
     4621 agaccgtcaa tggcgagatg aatagagata ccaaaacgcg ggatccacgg catgtcgaat
     4681 tcagactgcc actgcggaat tccggcggat tgcgtcagtg aataaccgcc ctgcaaccac
     4741 agttgcagcg acagcgccag cgtcaatccc atggtgatca gcgcgatcca gcgtggcacc
     4801 ttgacgccaa agcgttcggt ctgccagcac aggaagccgc caataaaggg aattaatatt
     4861 agccagggta gtaacatggc gatctttatt ccttgtaaaa gtcccgtcag gaccggattt
     4921 tcaacgaatt ctcacgacag aaaattctct tcggactggg ggtagtgtcg gatgcgccgc
     4981 gaaggcagct tatccgacct acgttcttat atgcccaggg gcaatcccac aatccttaac
     5041 tcaacgcagt accatcaaca gtgccagcac cacgaccgca ccgatgctca tggatgccac
     5101 ataccagcgc agataaccgt tctcgcttaa cagcagacct ttacctgcaa agcgggaaag
     5161 aaccgccggg atgttcatca ttgagttcag cggatcgcgt ttcagcaacc aggcaatacc
     5221 caggaacggc ttgacgaaca ctttgtcgta cagccagtca aagccccaag cgttgtacca
     5281 ccaggtactc agcaggcggc ccggcgcact gttggcgatg gaggtcacca gagaacgttt
     5341 acccagccac agccaggctg ccagcagaat gccgaccacc gcgaccacgc cagaggtaat
     5401 ttccagggtc aacatgctgc cgtgcgccag ttccgtcgtt tgcggaagca cgccctgcag
     5461 cggcggtaca atcagtgcgc caacgaaggt ggaaaggatc agcagcacaa tcagcggcag
     5521 gctgtgagtt acccctttca cggcgtgagc gtgaatttgt tcttttccgt ggaagacgat
     5581 gaaaatcata cggaaggtgt agagcgaggt cataaacgca ccgaccagac ctgccaccat
     5641 cagattgata tgaccattcg ccatcgcacc cgcgaggatc tcatccttac tgaagaagcc
     5701 cgcagtgacc agcggcagtg ccgacagtgc tgcgccgccc accaggaagc agagataaac
     5761 cagcggaata gatttacgca gaccgcccat cttgaagatg ttctgttcgt gatggcaggc
     5821 cagaatgacg gaaccggatg ccaggaacag cagcgcttta aagaacgcgt gagtcatcaa
     5881 gtggaaaatc gccgcatccc atgcctgcac gccaagcgcg aggaacatgt agccaatctg
     5941 gctcatggta gagtaagcga gaacacgttt gatgtcggtc tgtaccagcg cggcaaaacc
     6001 ggccagcagc agcgtaaccg ccccgacaat acccaccaga tgcagaactt ccggcgtcat
     6061 caggaacagg ccgtgggtac gggcgatcag gtagacaccc gcggttacca tcgttgcggc
     6121 gtggatcagc gcggagacag gcgtcgggcc cgccatcgcg tcagcaagcc atgtctgcaa
     6181 cggcaactgc gcagatttac cgaccgcacc gcccagcagc atcagcgtcg cccacatcag
     6241 catgttattg ccgtcagcaa agtgcgctgg tgccagttcc accatatcgc ggaagttcag
     6301 ggtgcccagt tcgttgtaaa gaatgaacag tgcgaaagcg aggaaaacgt cacccacacg
     6361 ggtcacgacg aacgctttca ttgctgctgc gccattcttc ggatcggtgt aatagaaacc
     6421 gatcagcaga taggagcaca ggcccacgcc ttcccagccg aggtacatca gcagcaggtt
     6481 atcggcgaga accagaacta ccatgctggc gatgaacagg ttggtgtagg cgaagaagcg
     6541 agagtagccc tcttcaccgc gcatgtacca ggaggcgtac atgtgaataa ggaaacccac
     6601 accagtgacc accgagagca tggtcagcga caggccgtcc agcaccaggt taaaaccgat
     6661 gttaaagtcg cctaccgaca tccacgtcca cagcggctgg ctgtatgcct gctcgccgtt
     6721 agcgaagaaa tcaacgccga taaaggcggt taccagcgcc gccaggccca cagagccaac
     6781 gccgacgatc gccgaaacgt tttcagacca gcgcccacgg gagaacgcca gcaggacgaa
     6841 gccaatcaat ggcaaaataa tggttaaggc aagcatgttc atccgcgcat ctcacttact
     6901 gaatcgatgt tcaggttctg gcgacgacgg tgaagttgca gcagcagcgc aaggccgata
     6961 ctcgcttctg ccgccgcgag gctgatggcg agaatgtaca tcacctgacc gtcggtctga
     7021 ccccagtagc ttccggcgac cacaaaggcc agcgcggagg cgttaatcat gatttccaga
     7081 ccaatcagca taaacagcag attgcgacgg ataaccagac cggttaagcc aagaacgaat
     7141 aagattgccg cgaggatcag tccatgttgt aaggggatca tgcgtgctcc tccgtttttc
     7201 ttttcgcgct gtcgtcttta cgattgctca gcacttcacc cgcacgctct tcacgaccga
     7261 cgtggaaggc cacaaccaga cccgcgagca gcagcataga agccagttcc accgccagta
     7321 cgtaaggccc gaacagcgta atacccactg ctttagcact gattggcgta ccgtcgatac
     7381 cctgatcgtt aacaccgagg atggcgtaaa caatcaccac cagcatgatg gccgacaaaa
     7441 ttgccgggcc aatccacacc tgcggtttca gccactggcg ttcctgttcg atttctgaac
     7501 cgcccaggtt gagcatcatc accacgaaca cgaacagcac cataatggca cccgcgtaga
     7561 cgataatttc cagcgcaccg gcgaagtaag cgcctagtga gaagaacacc ccggaaatcg
     7621 ccagcagcga aataatcagg tacagcagtg cgtgtaccgg attggtatgg gtgatcactc
     7681 gcaaggtcgc aagtatggct atcaggccac agatataaaa agcgaactcc attgcccctc
     7741 tccttacggt aacaggctct tgacgtcgat aggcttggct tcgttctctg cttcgccctt
     7801 atctttgccg tcgattgcca tacctgccat ccggtagaag ttatattccg ggtatttgcc
     7861 cggaccggag atcagcagat cctctttctc gtaaaccaga tcctggcgct tgtattcccc
     7921 catttcgaaa tccggggtta actgaatcgc cgtggtcgga caggcttctt cgcacagacc
     7981 acagaaaatg cagcgtgaga agttaatgcg gaaaaattcc ggataccagc gaccgtcttt
     8041 ggtttctgct ttttgcagcg agatacagcc gaccgggcag gctaccgcac agaggttaca
     8101 ggctacgcaa cgctcttcgc cgtccgggtc gcgggtcaga acgatacgac cacgataacg
     8161 gggcggcaga tagaccggct cttccgggta cattcgcgtt tcgcgtttgg cgaacgcgtg
     8221 caggccgatc atccagatac tacgaacctg ggtgccgaaa cctactaaca attcttttaa
     8281 ggtcatggtc ttattgcccc ttattgcgcc tgccagagaa tgacagccgc cgttaccagc
     8341 aagttgatca gcgtcagcgg caggcagatt ttccagccga aggacattac ccggtcataa
     8401 cgcggacgcg gtaacgacgc acgaatcaaa atgaacatca tcataaagaa cgcggttttc
     8461 agcgcgaacc agatgaatgg cggtaacaac gggccttgcc agccaccgaa gaacagcgtc
     8521 accatcaatg cagagatggt cacaatcccg atgtactcac ccacgaagaa cagaccgaac
     8581 ttcataccgg aatattcaat gtggtaacca tccgccagtt cctgctcggc ttccggctgg
     8641 tcaaacgggt gacggtgaca caccgccacg cccgcgatgg caaaggtaat aaaaccaaag
     8701 aattgcggga tgacgttcca cacatgcgcc tggctgttga cgatgtcggt catgttgaat
     8761 gaaccggcct gcgccaccac gcccatcagg gaaagcccga ggaacacttc gtagctcagg
     8821 gtctgcgcag aagcacgcat cgcacccagc agcgagtatt tgttgttact tgaccagccc
     8881 gcaaacagca ccgcgtaaac cgccagacct gccatcatca ggaagaacaa aatcccgatg
     8941 ttcaggtcgg caaccaccca acccggactg actggcacaa ttgcaaaggc cagcagcagc
     9001 gaggtaaagg caatcatcgg tgccagggta aagatgacgc gatccgagaa tttcgggatc
     9061 cagtcttctt taaagaacat tttgatcatg tccgcaacca gctggagcga accgccccag
     9121 ccaacacggt taggtccata acggttctgg aacagaccca gcaggcgacg ttcgccaaag
     9181 ctcatgaatg ccccacaggt gacaaccacc agcaggatca ccaccgcttt gaggatggtc
     9241 agcaggatct caatcagttc cggtgatatc caactcattg ttgtgcctcc ttgagatcct
     9301 caagatgcgc gccagccagc accggagcaa tgccggacat acccatcggc aagcccacct
     9361 gccctgccgt cagtccttcg gcgatttcaa ccggcagcgt gaccgtgttg ccatcgtaac
     9421 taaaggagac gcgtgtacct gcgttcacac ccaacttcgc ggcatccgct gggttgagtt
     9481 tgatgtacgg ctgcggcata cggctctgga agaccggagc acgctgtgac aattcatcgc
     9541 tgccaaacag gtggtagtac ggcgcgatac gccatttccc gtcctgcggc tggaagcgtg
     9601 ccggtacgct ggtgaagtaa tccagaccgt tttcgctggt ttcaaacaga cgcacgcccg
     9661 gatcgccaaa gcgcagtttg ccgcccactt cgtcctggaa tttgttccac gcctgcgggg
     9721 agttccagcc cggcgcccag gcaaacggca cttgcgaacg gtgcgcggtc ggctggttgt
     9781 taccttccat cgagaaggtg aacatggtgt caatatcctg cggctgacgc ggctcatgaa
     9841 cgctgatatt ggcgcgcatg gcggtacgac cgctgtaacg gtgcggttca cgggccagtt
     9901 tctgcccacg aatacggaat gtcgcatccg gcgcagcatc tttgataccc gccagttctg
     9961 ggattttcgc cacaacagcg tcaattacat ggtcgagctg cgtccagtcc acttcacggc
    10021 tcagcaaggt gctgtgcagc gagtgcagcc agcgccagct ttccagcatg acagttttgc
    10081 tgtcgtaata agccggatcg taaacctgga agaaacgttg ggcgcgacct tcattattaa
    10141 ttaccgtacc gtcgctttca gcaaagctgg cagaggaaag caccagatgg gcgttttcca
    10201 tgatcgctgt gcgttgatga tcaaccacca tcaccagcgg cgctttagcc agcgcagcat
    10261 tcacgcgggt agcagaagcg taacgatgca gatcgttttc caacaccacc accgcgtcgg
    10321 cgcgtccggt ttccagttcg gttaacgctt cttcaagcga accgccaccc ataatgccca
    10381 gccccatgct gttgacggaa cgggcaatca tggtgatacc aacgtcagcg ccgcgacctt
    10441 tcagggcttt cgcgacgtta gccgcagcct gaatcacctc tatgctaccg gcgttcgtcc
    10501 cggagataat caacggtttc ttcgcacctg ccagtgcctg cacgatgacg tcgattttgt
    10561 tttgcagctc aggttcgata ccgtcaaccg ctggtgcgga gttatccagc gcatgggcga
    10621 tggcaaaacc taaacgcgcc tgatcttcaa ccggtgcgcg gtaagtccac gccgcgatat
    10681 catccagacg agtgtcatca acgttggtaa caaacagcgg atgcttcgca cgttgaccga
    10741 tgttgaggat tgccgcaatc tgccagtcag ccactttctg tgctgcagcc atttcgcgcg
    10801 ctttaccttt caccgcctga cgcactgcca gtgcgacgcg cgcgccggtc tgggtaacgt
    10861 cttcgcccag caccagtacc gcatcgtaag attcgatttc gcgcagagcc ggagtataaa
    10921 tgccgccttc acgcagcact ttcagcgcca gttgcagacg ttcctgctca ccgtgagcga
    10981 taccggtgta gaagttttct tcgcccacca gttcacgcag cgcaaagttg ctttccacgc
    11041 tggcacgcgg agaaccaata ccgatcactt tcttcgactg acgcagaata tctgccgcgc
    11101 cctgcattgc ctgttcggcg ttgagggtaa tgaaatcatc gccacgacgc tgtactggct
    11161 gacgcggacg atccttcagg ttgacgtaac cgtaaccgaa acgaccacgg tcgcagagga
    11221 agtagtggtt taccgtaccg ttgtaacggt tttcgatacg acgcagttcg ccgtaacgtt
    11281 caccggggct gatgttacag ccgatggaac attgctggca gatgctcggc gcaaactgca
    11341 tatcccattt acggttgtaa cgctcggagt gcgttttgtc ggtaaatacg ccggtcgggc
    11401 aaatttcgac caggttaccg gaaaattcgc tttccagcgt gccgtcttcc gggcgaccga
    11461 agtagacgtt gtcgtgcgcg ccataaacgc ccagatctgt accgtcagcg taatctttgt
    11521 agtaacgcac acagcggtag caggcgatgc agcggttcat ttcgtgagag atgaatggcc
    11581 ccaaatcctg attacggtgg gtacgtttgg tgaaacggta gcgacggaag ctgtgtccgg
    11641 tcatcacggt catatcctga agatggcagt taccgccttc ttcacatacc ggacagtcgt
    11701 gcgggtggtt ggtcattaac cactcgacca cactttcacg gaactgtttc gcttcttcgt
    11761 cgtcaatgga aataaaggtg ccatcggaag ccggtgtcat acaggacatc accaggcgac
    11821 cacgcgtgtc ttccgcgttt tggtattgct tcaccgcaca ctggcggcaa gcaccgacgc
    11881 ttcccagcgc cggatgccag caaaagtaag gaatatcaag gcccagagac agacaagctt
    11941 ccagcaggtt gtccgctccg ttgacctcgt attctttgcc gtctacatga attgtagcca
    12001 ttagcatgct tccagttttc tcggtcaggg accgagcgtt aatcgaaatt cggttaccag
    12061 cgctctttca gcaggttcgg ctgaatccca ttaatcaaat gggtattgct gaacggctgt
    12121 ttgattcccg cctcaaattc ttcgcggaaa tatttgatgg cgctctgtaa cggctccact
    12181 gcaccaggtg cgtgggcaca gaaagtttta cccgggccta agaatcgaca cagttgctca
    12241 agtgtttcga tatcgcccgg ctggccttcg ccacgctcca gcgcacgcag aattttcacg
    12301 ctccacggca gaccgtcgcg gcacggcgta caccagccgc aggactcacg ggcgaaaaac
    12361 tcttccaggt tacgcaccag cgacaccatg ttgatctcat ggtcaaccgc catcgccagc
    12421 gccgtaccca gacggctgcc cgctttaccg atactttcga attccatcgg cagatcaagg
    12481 tgcgcttcgg tcaggaagtc agtccccgcg ccgcctggct gccaggcttt aaatttcaga
    12541 ccatcacgca taccaccggc gtaatcttcg aggatctcgc gtgcggtggt gccgaacggc
    12601 agttcccaca gtcccggatt tttcacccga ccggagaagc ccatcagctt ggtgccagca
    12661 tctttacttt tcgagatgtt ctgataccac tccacgccgt tagcgaggat cgccggaacg
    12721 ttacacaggg tttcgacgtt gttgacacag gtcggtttgc cccatacgcc ggaggttgcc
    12781 gggaaggggg gtttcgagcg tgggttagca cgacgccctt ccagggagtt gattaacgct
    12841 gtttcttccc cgcagatgta gcgccctgcc ccggtatgga cgaacagttc gaagtcgaaa
    12901 cctgttccca taatgttttt gccaagcaag cccgcttcag tggcttcggc aatggcgcgg
    12961 cgcagattta ctgccgcttc gatatattcg ccacgcagga agatgtagcc acggtaagct
    13021 ttcagcgcaa acgcggagat gagcatacct tccaccagca ggtgcggcag ttgctccatc
    13081 aacaggcggt ctttataggt gcccggctcc atttcatcgg cgttacacag caggtaacgg
    13141 atgttcatgg attcgtcttt cggcatcagg ctccacttca ggccagtcga aaagcccgcg
    13201 ccgccgcgcc ctttcagacc agcgtctttt acctgattaa cgatttcgtc cggagacagc
    13261 ccggtcagcg ccttacgcgc cccttcgtaa ccgtttttgc tgcggtattc gtccagccac
    13321 actggctgtt tgtcatcgcg cagacgccag gtcagcggat gcgtttcggg agtacggata
    13381 atgtttttca tttataccgc tccagcagtt cagggatcgc ttccggggtc agatgcgcgt
    13441 gagtgtcctc atcgatcatc atgtttggcc ctttatcaca gttccccagg cagcaagttg
    13501 gcagcagcgt aaagcggcca tcaaatgtcg tttgccctgg tttgatgttc agctttttct
    13561 cgagcgccgc ctgaataccc tgatacccgt tgatatgaca gaccacgctg tcgcaataac
    13621 ggatcacatg gcgaccaacc ggctggcgga agatctgact gtagaacgtt gccacacctt
    13681 cgacgtcgct tgccggaata cccagcacat cggcgatcgc gtggatcgca ccatccggca
    13741 cccagccacg ctgcttctga acgattttca gcgcttcaat ggacgccgca cgcgggtctt
    13801 cgtagtggtg catctcgtgc tcaatcgctt cacgctctgc cgcactcagc tcaaaagcct
    13861 cggtttgtgg ttgttgattc tcgtgcataa ttagcggtcc acatctgaca taacaaaatc
    13921 gatactgccc agataaacaa tcaggtcaga caccaggctg ccgcggatcg ccgccggaat
    13981 ttgctgcaaa tgcgcataac tcggcgtgcg gatacgagtg cgataactca tggtgctacc
    14041 gtcgctggtc aggtagtaac tgttgatccc cttggtcgcc tcaatcatct ggaaagattc
    14101 attggcaggc atcaccggcc cccacgacac ttgcaggaag tgggtgatca gggtttcgat
    14161 atgttgcagc gtgcgctctt tcggcggcgg cgtggtcagc gggtgatccg ctttgaacgg
    14221 gccttccggc atgttgttga ggcactgctc aagaatgcgc agactctggc gcagctcttc
    14281 cactttcagc attacgcggg tgtagcagtc agaaacacca ccaccaaccg ggatttcaaa
    14341 gtcgaagttt tcatagccag aataaggacg cgccttacgc acgtcgaagt cgatcccggt
    14401 agcacgcagg cccgcgccag tggtgcccca ctccagcgcc tctttcgcgc cataggcggc
    14461 aacgccctgg gaacgacctt tcagaatggt attttgcagc gccgctttct cgtaagacgc
    14521 cagacgtttc ggcatccagt cgaggaactc acgcagcaga cgatcccagc cgcgcggcag
    14581 gtcgtgcgct acgccgccaa tacggaacca cgccgggtgc atacggaaac ccgtgatcgc
    14641 ttccaccaga tcgtaaattt tctgacgatc ggtaaaggcg aagaacacgg gcgtcattgc
    14701 gccgacgtcc tgaataaagg tcgagatgta cagcaggtgg ctgttgatac ggaacagttc
    14761 ggagagcata acgcgaatga cgttaacgcg atccggcacg gtgatcccgg ccagtttctc
    14821 taccgccagc acgtaaggca tttcgttaac gcagccgccg aggtattcaa tacggtcggt
    14881 atacggaatg tagctgtgcc aggactggcg ctcgcccatt ttctccgcac cacggtggtg
    14941 gtagccgatg tctggtacgc agtcgacaat ctcttcgccg tcgagttgta aaacgatacg
    15001 gaaagccccg tgcgccgacg ggtggttcgg accgaggttg aggaacatga agtcctcatt
    15061 ttcggtgccg cgcttcatcc cccactcttc cggtttgaag gtcagggctt ccatctccag
    15121 atcctgtttg gctttggtca gctcaaacgg cgagaattcg gtagcgcgtg ccgggtaatc
    15181 tttacgcagc gggtgacctt tccaggtttg cggcatcatg atgcggcgca ggttcgggtg
    15241 accgtcgaaa gtaatgccaa acagatccca ggtttcacgc tcataccagt tagcgttcgg
    15301 gaacagtttg gtgaaggtcg gtacgtgcag gtcgttttct gccagcgcca ccttcagcat
    15361 gatgtcgcgg ttacgatcga tagaaatcag atggtagaaa acggaaaaat ccgcggcagg
    15421 taacccttcg cggtgtgtgc gcagacgttc gtccatgccg tgtaagtcaa acagcatgac
    15481 gtaaggtttc ggcagtttct ttaagaaatc gccaacttcc agtaattgtt cacgcttgat
    15541 ccacacaacg ggaaccccgg tgcgagtcgc ctgaacagta aaggcatccg gcccaaaacg
    15601 gttgcgcagt tcgccaatta ccggatcatc aagatgatcg cgggtctgcc aggcgggttc
    15661 ttgcgcggtt aagtcggtca tattgttcac cattgcaaat ggtccgtggt gactgtcggc
    15721 gcagtaagct tcgcgaaata gatttaggaa taagcgaaga aaaatccctt tgccgacagg
    15781 cgcaaattaa atctcgtcag gtgtacgcag gttggttacg gcaatgcgtt caccgcgctt
    15841 gcgttcgcgc tctgattgca tattggcgcg ataaacgccc tgatcgccaa ccacccagga
    15901 gagcggacga cgttctttgc cgatagattc ctgcaacagc atcagtgcct gcatgtacgc
    15961 ttcaggacgc ggcgggcagc ccgggatata cacatcaacc gggatgaatt tatcgacgcc
    16021 ctgcacaacg gaataaatat cgtacatacc accagagttg gcacaggcac ccattgagat
    16081 aacccatttt ggttccagca tctggtcata cagacgctga ataaccggtg ccattttggt
    16141 aaagcaggtt cctgcaacca ccatcaggtc agcctgacgc ggcgaagcac gcaatacttc
    16201 tgcgccaaaa cgcgccacgt catgcaccgc ggtaaacgaa gtcaccatct caacgtaaca
    16261 gcaggaaaga ccgaagttat acggccaaat tgagttttta cgaccccagt taaccatgtc
    16321 attgagcttg cccataaaca cgtttttgtt aacttcttgc tccagagggt cggttacgat
    16381 ctcctgcttt tgcagggggt aacggtcgtt ctcaccgttg ggatctatgc gggtgagcgt
    16441 ataatccatc ttaatgcctc gcggttagcg ttgacgatta gcgatactgt tcgtttccgg
    16501 gttcatacgc tcgcggcgtg aacgcgcggg cgtccagtcc agcgcgccaa tacgcaccag
    16561 ataaaccaga cctgccagta acacaaaaat aaaaattgca gcttccacaa agcctaccca
    16621 gccgctttcg cggatagagg ttgaccatgc gaacagatac agcgcttcaa cgtcgaagat
    16681 aacgaagaac atggccacca gataaaactt ggcggacagg cgtaagcggg cggagccgac
    16741 cgagtcgata ccggattcaa acggcacgtt tttcgacctc gcgcgtgcgc gaccgcctaa
    16801 aaaccaaccg cctaccagca tcaggcaaca caggccaatg gcaacgataa gaaagatagc
    16861 gaatgcccag tgatgagcga tgacttcagt ggatgttgac atactcattg cttactcatc
    16921 aaaagtagcg ccagattcac tgctcttcac ggcagatgga cgccacatcg attcatgggg
    16981 aggaataaaa aaaaccttac aataactgta gaaattcttt tatacagcta attgatgtgg
    17041 tcttttactc ctttctataa ccttttgtca actttaacaa aagcttcttc acattagttt
    17101 acataatatc aacaccatta gcatttaatg ccctttcacc ccagatcctt gacgactcca
    17161 ggataattag atgttgttga atcgtgtccg ttgtgaagca atggaaaaaa tacgggtcta
    17221 ttttgacagg aatttgtgtc gattcctccc cccaaaagag agtattttct tgatctgtga
    17281 cacgcttttg ttattccata acaaaaacgc agcaacaaat ttacgtattt tttaacatca
    17341 ttgtagcagg tgattttttt caggcgatta tttgtgcatt cgggacgtga atctctggtg
    17401 attgaaaaat aaacagtttt gtacgttctg cactatgcga ggaaggattt ttactaaaaa
    17461 aaagccgctg gggtttaaaa cacccccagc ggctcgtttt ttacactatt gtctcaggaa
    17521 ttatctatcg tccgtcgatt actcgatatc cctttcaatc aacaatgaat catccccttc
    17581 cggagcagac attgggctgt attgccacgg attatggtag ctctccatcg cctgatagat
    17641 cacctgggcc agctcattat tactggacgg gtcatagcac agcaaatatt cggtatcagg
    17701 tagcggcggt aagccatcta ctccactcag cacacgcagg tccgggctca tcatctcaac
    17761 cggccttgcc gtcacgccaa gaccggcttt cactgccgca cgaacggccg gaagcgtcga
    17821 ggcgacataa gccagtcgcc atggaatatc tactttatta agcgtcgcca gcaccatatc
    17881 gcgaaacgga cttggatcat ccagcaatac aagagggatc ggctcgcctt tttgcaatac
    17941 gtattccgct gcgcagtacc agtgtgttgg cgaggtacgc aggttcaatg ccttaaacgc
    18001 tgaaggacga tgggtggtta ccatcaaatc cacttcctgg gattccagca tttctgccat
    18061 ataggcatta cgtttaacgc ggacatccag cgctaatttc ggataaaccg aactcacgcg
    18121 atttaacagg aaaggtaaga tcgtatcggc agattcatct gaagcaccga tagttaacac
    18181 gccctgaaga ttactgaaca ttaatgatga gcaggcctca tcattaaaac gcaggatttt
    18241 cctggcgtaa ccaagaagtt gaatgccatg ttcagttaac agtttgttgc gaccgtgacg
    18301 agcgaacagt tctttcccaa cgagttgttc cagacgctgc atttgctgac ttacggcgga
    18361 ctgagtacga cacacagcgg cagctgcggc agcaaaagtg ttcagatcgg caacagcaac
    18421 aaatgttctc agcagatcga ggtcgaggtt aattatcgga cgatttgcac ttatcatata
    18481 ttatcactta ctggcggctc atactgagct ggttaatgct gtgcacacac aaacaagcaa
    18541 ttccatttgt aatgtgcctc cctggcagtt tcatccggaa cccggacgaa agtaaaaatg
    18601 catatgagtt gcactaaaaa agcgactcac attgttccgt tataatgcct gaagtagatc
    18661 acagaatata tcttcaggga tcgcatatct attaagttac tcactctttt ctatttatga
    18721 catgcgcgtg tttgtataaa tgtaaatgtg agtccttgtt ccactctcgt gcagcatcgc
    18781 tggtcatacg cgaacacgta caaacagcaa tggtgaagcg gcatcaagag cagggatccg
    18841 tcatttatgc gagcatttta ccccaaaaac cttttattta taagggtcat tgcgaattat
    18901 ctgatgcaaa gttatgttat gttaggcaaa gtaatcttct attattaata agcacatcaa
    18961 aacctttttg aatattaaat aataattaat tagcatcatc ctcattcatt aattccgctt
    19021 aacaatagtt tcacacctta cccccattgc gacacctcgg aaatcatcac gctgttaaac
    19081 tctccgtaat gcggaaatat catcatcgcg tttcattagt gaattcttaa atgaggcatt
    19141 ttcacacaat tatcttacag ataaaaaaac cagacttgca attaagaatt agaacaattc
    19201 actatataac attgcatgta aagcatataa gcctcattat ttgtcattat taagttatta
    19261 acagcacaat cgagccttcc cctctggcaa aatcttattc tgcagacctt caaaacaccg
    19321 tcctggggga gtacattgtt ctaagctgac ttccacggca gggagtggcg ataacagcaa
    19381 aaaaggtcaa gattcatgtc ccccattgaa aaatccagca aattagagaa tgtctgttat
    19441 gacatccgtg gtccggtgct gaaagaagca aaacgcctgg aagaagaagg taacaaggta
    19501 ctgaaactga acatcggcaa cccagcccca ttcggttttg acgcgccaga tgaaatcctc
    19561 gttgacgtga tacgcaacct gcctaccgct caagggtatt gcgattccaa agggctttac
    19621 tccgcgcgta aagccatcat gcagcactac caggctcgtg gcatgcgtga tgttaccgtg
    19681 gaagatattt acatcggcaa tggtgtatcg gagcttatcg ttcaggcaat gcaggcattg
    19741 ctgaacagcg gggacgaaat gttggttcct gcaccagatt acccactctg gaccgcggcg
    19801 gtttcgcttt ccagcggtaa agcggtgcat tatctttgcg atgaatcctc tgactggttc
    19861 ccggacctcg atgatattcg cgctaaaatt acgcctcgta cgcgtgggat tgttattatc
    19921 aacccaaata acccaaccgg cgcggtatat tccaaagagc ttttaatgga gattgtggag
    19981 attgcacgtc agcataatct cattatcttc gccgatgaaa tttatgacaa aattctctac
    20041 gacgatgctg agcatcactc aattgcgccg ctggcacctg acctgctgac cattaccttt
    20101 aacggactgt cgaaaacgta ccgcgttgca ggcttccgtc aggggtggat ggtgttgaac
    20161 gggccgaaaa aacacgccaa aggctacatc gaaggtctgg aaatgctggc ttcaatgcgc
    20221 ctgtgtgcta acgttcctgc gcaacacgcc attcagaccg cgctgggtgg ttatcagagc
    20281 atcagtgaat ttattacccc tggcggtcgt ctttatgagc agcgtaaccg cgcgtgggaa
    20341 ctgatcaacg atattccggg cgtttcctgc gtgaaacctc gtggtgcgct gtatatgttc
    20401 ccgaaaatcg acgccaaacg ctttaacatt catgacgatc agaaaatggt gctggatttc
    20461 ctgttgcagg aaaaagttct gttggtgcaa gggacggcat tcaactggcc gtggccggat
    20521 cacttccgca ttgtcacgct accgcgtgtc gatgatatcg agctgtcttt gagcaagttc
    20581 gcgcgtttcc tttctggtta tcatcagctg taatcttaat ttcactgccg gagattgcat
    20641 ccggcagtgt tatcccgcca caatgacctg atgatgtcat catacgtaag gtcactatga
    20701 aacagagcca tttttttgcc catctctccc gcctgaaact cattaaccgc tggccgctaa
    20761 tgcgcaacgt gcggacggaa aatgtgtccg aacacagttt gcaggtagcg atggtcgccc
    20821 atgcgctggc agctatcaaa aatcggaaat ttggcggtaa tgtcaacgcc gaacgtatcg
    20881 ctttactggc gatgtaccac gatgcctcag aagtgctcac cggcgatctc cctactccgg
    20941 tgaaatactt caatgcgcaa atcgctcagg agtacaaggc tattgaaaaa atcgctcagc
    21001 agaaactggt cgatatggtt ccggaagaac tgcgggatat ctttgcgccg ttaattgacg
    21061 agcatgcata tagcgatgaa gaaaaatcgc tgatgaaaca ggcagatgca ctgtgtgcat
    21121 atctgaaatg tctggaagaa ctcgcggccg gaaataatga attcttgctg gcaaaaacgc
    21181 gactggaagc gacgcttgaa gcgcgtcgca gccaggagat ggactacttc atggaagtat
    21241 ttgttcccag cttccatctt tcgctcgatg agattagcca ggattcaccg ctgtaagcag
    21301 ccggagtctg cgtcgcatca gtcaataagc gccggatgcg acatcaggct cttgtcaaaa
    21361 cggaaacagc atcgggatca tcaccacaca aaccgccatc acgataatgg tgaacggtac
    21421 ccccaacttc acaaagtcac tgaagctgta atttcccgga cctaaaacca gggtgttaac
    21481 aggtgaagac accggtgtca taaaggcggc ggatgctgcc atcgccacga ccatcgcaaa
    21541 tggataaggc gacactccca tcgttttggc agcagccagc gcaatcggag ccatcaacac
    21601 cgccgtcgcg gtattagaga taaacagccc aataaccgcc gacaagacaa acaaacaccc
    21661 cagcatcata tgtggcccat aaccgccgcc aatatccatc agccctttca ccgccagcgc
    21721 gacacctccc gttttctgta atgccacagc aaacggcatc atcccaacgg tcaaaataat
    21781 gctcggccag tgaatggatt tataggcgct ttcagcatct atacagcgga atttccccat
    21841 cagcaggcag gcgatgatag cggcaacagg attaggaatt tcatttgtca gcattaacgc
    21901 caccattagc accagacaga aaatggcatg gggtgcctgg ctgtgcgcgg gcgatgcttc
    21961 actcacctct tccggtaagt tcagcgctac gaagtcgcgg ccctgtttgg ccagcatacc
    22021 gatcagtttc cagttaccca caaccaggat gatatcgccc agcagcagag gctcatccgc
    22081 cagcgaacct tccagcgcca cgccattgcg cttgagcccc accacattca gtccgtagcg
    22141 ggtacgaaaa ccaatttcgc gcaccgattt accaatcagt tctgactcag gaattaatga
    22201 aatctctgcc atgcccacat caagggcctg gtcagaaaaa tactcgccgc gcagtaccat
    22261 cggctccagc aattgctcac tacaaaattg ccggagatcg acatcagccg cagacatatc
    22321 aataagcaaa acgtcacgcg cgcgaaattc agaaacccca ttaacgttca cgataacgcg
    22381 acgaaaacgc cgccagcgtt caacaccgat gacgttagcg ccataacgct cacgtaattt
    22441 gagatcatcc agccgttgac ctatcattgg cgatccgggg cgaatagcca gacgtcgcgc
    22501 acgcccggtc agtcgatatt cacggataag atcgcgaaag gttcgacgcg tccagccttc
    22561 gcgctgcggg gtctgggtat cccctttcag catgaaacgc atcactaaca tatacaaaat
    22621 acccagcacc agcacgacca ggccaatagg tgttacgcta aagaaactga agccgtgata
    22681 gccttcacgc agcaattcac tgttgactac caggttcggc ggcgtcgcca ccagcgtcat
    22741 catgccgcta atcagcccgg caaaacttaa cggcatcatc agacgtgacg gcgaagtttg
    22801 catacgcatg gcaacgctta acaccacggg aataaagata gcgacaacgc tggttgaact
    22861 cataaacgcg ccaagccccg cgacggtcag catcaacaaa accaacattt tgatttcact
    22921 gttgcccgcc actttgacca gccatgttcc cattacggtg gcaacaccgg tacggaccaa
    22981 accatcgcca ataataaaca aggcggcaat caggacaacg ttaggatcag aaaagccgga
    23041 aaatacttcg gggaccgtca gcgttccgct taatgcaaac gcgacaataa caaacaaagc
    23101 gaccgcatcc atacgcactc tgcccgtcgc aaacaagacg atggcaacgg ccagtaatga
    23161 aagaacccaa atcaattcac cgttcacaac ttatccttgt taattgaggg ggatgacttg
    23221 attctgccat aaaaaagccc cgacgagacg gggctaaatc atgatcaggt gtttcactga
    23281 ataataacat cgccatttgg ctgtttggtc acagtaattt gctccagact gtggaggacc
    23341 aaatcggcct cattcaggcg cggggtatct gccggagcgt taaccgcaat gacatggcaa
    23401 cccgccgcca ggccagaaag cacgccagcg ggagcatctt ccaccaccac acactcctgc
    23461 ggcgcaagcc ccagcagctg cgcacctaac agatacgcat caggttctgg ttttccgcgc
    23521 ttcactcgct cagcggttac aaacacctct ggtgcgggaa gccccgctat tttatggcgc
    23581 gctcgcgcta ccggcatgga gccagaagtc acaatggccc acggaatacc tgctttattc
    23641 aaatgattga gtaaggcgat tgccccagga agcgcggtaa taccttcggt ttccgtggcc
    23701 tcgatttgct ccagacgcgt aaactcggcg gcaatatcag cctcggattt gcccgccata
    23761 aaatggcgca aagaggtgat cgcctgttta ccgtgaatga aagccagcac ctcttccggc
    23821 gctaacccat gacgtctggc ccagttgctc cacgcccgtt ctaccgcagg cagggaatcc
    23881 accagcgttc catcaagatc aaacagaaaa cctttgcacc gcacgcgggc ctcctcaggc
    23941 gttgataatt tgattaattt cgttggcgct caaatggtac tgacgcgggc aggcatgcca
    24001 cacattaagc atgcgctgat atttttccca cattggcgtc tgggcgttaa aaccatgagt
    24061 cccagcgtca aaatgggtat agcgcccttc cacattaacc ataaagcgga cataaccgag
    24121 gtaacgtgct tcagtggcgg cgtcaaagcc gaggaaggtg acacgacgtt catcgatgga
    24181 ttgctggtcc tgcaaattag accaggaaac atacaacgca tgatacatat ccataatgtc
    24241 gatgatagtg cggcaggttt cttctttcag ctcgccaaac tcgcgatcca attcacgcat
    24301 ctgtaatccg taaccacgct caataattgt ttgcaggcga cggtaacgtt cagcatttgc
    24361 cggatcgagc atagtcatca tcttgtactg attagacaaa ataagacgtt gcgcgttggt
    24421 catttccatt gttgactcct gtatcactct actacggtga aaaaaaagaa ggctgagtat
    24481 gccttctttt atatgcgtaa tcaggggtca attacaaatc atcaaggaaa gttttatcca
    24541 gttgtttgaa ggcgcgctta agcgtgtcag ctaatgcctg gtaatcaggc ttgccttcaa
    24601 cgggtgccaa cacctgtcca gactcctgca atttaccgcg aacttcataa aaccagttaa
    24661 ggattgcagg gggtaatggc gttacagaac gcttgcccag ccaccacaat ccctgcatgg
    24721 gtaaacttaa ggcgaacagt gcagtggcaa ctgccggccc aagcagaccg cccagggcaa
    24781 tctgccagca gagagtaaat acggcgatcg gcggcataaa acggatcgca taacgcgtca
    24841 tcttgataac gcgattttcg acaaagaccg gagcaaggcg tttttccagt ggccacgtct
    24901 ttgagtaatg ctgtccgcgg cgaaacaagc taaaaaaatt aacagaacga ttatccggcg
    24961 ttgacatgct tcacctcaac ttcacatata aagattcaaa aatttgtgca aattcacaac
    25021 tcagcgggac aacgttcaaa acattttgtc ttccataccc actatcaggt atcctttagc
    25081 agcctgaagg cctaagtagt acatattcat tgagtcgtca aattcatata cattatgcca
    25141 ttggctgaaa attacgcaaa atggcataga ctcaagatat ttcttccatc atgcaaaaaa
    25201 aatttgcagt gcatgatgtt aatcataaat gtcggtgtca tcatgcgcta cgctctatgg
    25261 ctccctgacg tttttttagc cacgtatcaa ttataggtac ttccatgtcg agtaagttag
    25321 tactggttct gaactgcggt agttcttcac tgaaatttgc catcatcgat gcagtaaatg
    25381 gtgaagagta cctttctggt ttagccgaat gtttccacct gcccgaagca cgtatcaaat
    25441 ggaaaatgga cggcaataaa caggaagcgg ctttaggtgc aggcgccgct cacagcgaag
    25501 cgctcaactt tatcgttaat actattctgg cacaaaaacc agaactgtct gcgcagctga
    25561 ctgctatcgg tcaccgtatc gtacacggcg gcgaaaagta taccagctcc gtagtgatcg
    25621 atgagtctgt tattcagggt atcaaagatg cagcttcttt tgcaccgctg cacaacccgg
    25681 ctcacctgat cggtatcgaa gaagctctga aatctttccc acagctgaaa gacaaaaacg
    25741 ttgctgtatt tgacaccgcg ttccaccaga ctatgccgga agagtcttac ctctacgccc
    25801 tgccgtacaa cctgtacaaa gagcacggca tccgtcgtta cggcgcgcac ggcaccagcc
    25861 acttctatgt aacccaggaa gcggcaaaaa tgctgaacaa accggtagaa gaactgaaca
    25921 tcatcacctg ccacctgggc aacggtggtt ccgtttctgc tatccgcaac ggtaaatgcg
    25981 ttgacacctc tatgggtctg accccgctgg aaggtctggt catgggtacc cgttctggtg
    26041 atatcgatcc ggcgatcatc ttccacctgc acgacaccct gggcatgagc gttgacgcaa
    26101 tcaacaaact gctgaccaaa gagtctggcc tgctgggtct gaccgaagtg accagcgact
    26161 gccgctatgt tgaagacaac tacgcgacga aagaagacgc gaagcgcgca atggacgttt
    26221 actgccaccg cctggcgaaa tacatcggtg cctacactgc gctgatggat ggtcgtctgg
    26281 acgctgttgc attcactggt ggtatcggtg aaaatgccgc gatggttcgt gaactgtctc
    26341 tgggcaaact gggcgtgctg ggctttgaag ttgatcatga acgcaacctg gctgcacgtt
    26401 tcggcaaatc tggtttcatc aacaaagaag gtacccgtcc tgcggtggtt atcccaacca
    26461 acgaagaact ggttatcgcg caagacgcga gccgcctgac tgcctgattt cacaccgcca
    26521 gctcagctgg cggtgctgtt ttgtaacccg ccaaatcggc ggtaacgaaa gaggataaac
    26581 cgtgtcccgt attattatgc tgatccctac cggaaccagc gtcggtctga ccagcgtcag
    26641 ccttggcgtg atccgtgcaa tggaacgcaa aggcgttcgt ctgagcgttt tcaaacctat
    26701 cgctcagccg cgtaccggtg gcgatgcgcc cgatcagact acgactatcg tgcgtgcgaa
    26761 ctcttccacc acgacggccg ctgaaccgct gaaaatgagc tacgttgaag gtctgctttc
    26821 cagcaatcag aaagatgtgc tgatggaaga gatcgtcgca aactaccacg ctaacaccaa
    26881 agacgctgaa gtcgttctgg ttgaaggtct ggtcccgaca cgtaagcacc agtttgccca
    26941 gtctctgaac tacgaaatcg ctaaaacgct gaatgcggaa atcgtcttcg ttatgtctca
    27001 gggcactgac accccggaac agctgaaaga gcgtatcgaa ctgacccgca acagcttcgg
    27061 tggtgccaaa aacaccaaca tcaccggcgt tatcgttaac aaactgaacg caccggttga
    27121 tgaacagggt cgtactcgcc cggatctgtc cgagattttc gacgactctt ccaaagctaa
    27181 agtaaacaat gttgatccgg cgaagctgca agaatctagc ccgctgccgg ttctcggcgc
    27241 tgtgccgtgg agctttgacc tgatcgcgac tcgtgcgatc gatatggctc gccacctgaa
    27301 tgcgaccatc atcaacgaag gcgacatcaa tactcgccgc gttaaatccg tcactttctg
    27361 cgcacgcagc attccgcaca tgctggagca cttccgtgcc ggttctctgc tggtgacttc
    27421 cgcagaccgt cctgacgtgc tggtggccgc ttgcctggca gccatgaacg gcgtagaaat
    27481 cggtgccctg ctgctgactg gcggctacga aatggacgcg cgcatttcta aactgtgcga
    27541 acgtgctttc gctaccggcc tgccggtatt tatggtgaac accaacacct ggcagacctc
    27601 tctgagcctg cagagcttca acctggaagt tccggttgac gatcacgaac gtatcgagaa
    27661 agttcaggaa tacgttgcta actacatcaa cgctgactgg atcgaatctc tgactgccac
    27721 ttctgagcgc agccgtcgtc tgtctccgcc agcgttccgt tatcagctga ctgaactggc
    27781 gcgcaaagcg ggcaaacgta tcgttctgcc ggaaggtgac gaaccgcgta ccgttaaagc
    27841 agccgctatc tgtgctgaac gtggtatcgc aacttgcgta ctgctgggta atccggcaga
    27901 gatcaaccgt gttgcagcgt ctcagggtgt agaactaggt gcaggcattg aaatcgttga
    27961 tccagaagtg gttcgcgaaa gctatgttgg tcgtctggtc gagctgcgta agaacaaagg
    28021 catgaccgaa accgttgccc gcgaacagct ggaagacaac gtggtgctcg gtacgctgat
    28081 gctggaacag gatgaagttg atggtctggt ttccggtgct gttcacacta ccgcaaacac
    28141 catccgtccg ccgctgcagc tgatcaaaac tgcaccgggc agctccctgg tatcttccgt
    28201 gttcttcatg ctgctgccgg aacaggttta cgtttacggt gactgtgcga tcaacccgga
    28261 tccgaccgct gaacagctgg cagaaatcgc gattcagtcc gctgattccg ctgcggcctt
    28321 cggtatcgaa ccgcgcgttg ctatgctctc ctactccacc ggtacttctg gtgcaggtag
    28381 cgacgtagaa aaagttcgcg aagcaactcg tctggcgcag gaaaaacgtc ctgacctgat
    28441 gatcgacggt ccgctgcagt acgacgctgc ggtaatggct gacgttgcga aatccaaagc
    28501 gccgaactct ccggttgcag gtcgcgctac cgtgttcatc ttcccggatc tgaacaccgg
    28561 taacaccacc tacaaagcgg tacagcgttc tgccgacctg atctccatcg ggccgatgct
    28621 gcagggtatg cgcaaaccgg ttaacgacct gtcccgtggc gcactggttg acgatatcgt
    28681 ctacaccatc gcgctgactg cgattcagtc tgcacagcag cagtaatctc gtcatcatcc
    28741 acagctttgc gctgcggata tctgaaccgg aaataatcac tatttccggt tttttattct
    28801 cttaatttgc attaatcctt tctgattatc ttgcttaact gcgctgcatc aatgaattgc
    28861 gccatctcac tttgcatact taccactttg ttttgtgcaa gggaatattt gcgctatgtc
    28921 cgcaatcact gaatccaaac caacaagaag atgggcaatg cccgatacgt tggtgattat
    28981 cttttttgtt gctattttaa ccagtcttgc cacctgggta gttccggtgg ggatgtttga
    29041 cagtcaggaa gtgcagtatc aggttgatgg tcaaacaaaa acacgcaaag tcgtagatcc
    29101 acactcattt cgcattctga ctaacgaagc aggcgaacct gagtatcacc gcgtacagct
    29161 gttcacgacg ggcgatgaac gcccgggcct gatgaacttc ccgtttgaag ggttaacctc
    29221 aggatcgaaa tacgggacag ccgttggcat catcatgttt atgctggtga ttggcggcgc
    29281 gtttggcatt gtgatgcgta caggaaccat tgataacggt atcctggcgc ttattcgcca
    29341 tacccgcggg aatgaaattc tctttattcc tgcgctgttt attctgtttt cacttggcgg
    29401 cgcggtattt ggtatgggag aagaggccgt cgcctttgcc attatcatcg caccgctaat
    29461 ggtccggctg ggctatgaca gtattaccac cgtcctggtg acttatattg ccacgcaaat
    29521 cggttttgcc agttcgtgga tgaacccgtt ttgtgtggtt gttgctcagg ggattgccgg
    29581 cgttccggtg ctttctggct ccgggttgcg catcgtggtg tgggttatcg ccactctgat
    29641 tggcctgatc tttaccatgg tgtacgcctc acgagtgaaa aagaatcctc ttctgtcacg
    29701 cgtgcatgag tccgaccgct tctttcgtga aaagcaggcg gatgttgaac aacgtccgtt
    29761 tacctttggt gactggctgg tattgattgt cctgacagcc gtaatggtct gggtgatttg
    29821 gggcgtgatc gttaatgcct ggtttattcc agaaattgcc agccagttct tcaccatggg
    29881 tctggtgatt ggcatcatcg gcgttgtttt ccgccttaac ggcatgacgg ttaataccat
    29941 ggcttcatcc tttaccgaag gggcgcgaat gatgatcgcc cctgccctgc tggtgggttt
    30001 cgccaaaggg attttgctgc tggttggtaa tggtgaagca ggtaatgcca gcgtgttaaa
    30061 tactatcctc aacagcattg ccaatgccat tagcggtctg gacaacgcgg tcgcggcctg
    30121 gtttatgttg ctcttccagg ctgtatttaa tttcttcgtg acgtccggtt ctggtcaggc
    30181 ggcattaact atgccgttac tggcaccgct tggcgatctg gtcggtgtta accgtcaggt
    30241 taccgtgctg gcgttccagt ttggtgatgg cttcagccac atcatttacc caacctcagc
    30301 ttcgttaatg gcaacgctcg gtgtgtgccg ggtggacttc cgtaactggc tgaaggtggg
    30361 cgcgaccctg cttggactgc tgtttattat gtccagcgtc gtagtgatcg gcgctcagtt
    30421 gatgggctac cactaaaaat gttaagagcc gcaattgcgg ctcttttttc attctgccgt
    30481 ttcagtctct acggcttcat ttttggcatt gcgcttcatc cacaacgcca gcgctttcag
    30541 cgagtctgga gtgaactcat cgcagcgtgc ggtgatttct tccggcgtca gccagcaaac
    30601 tccactgact tcatcttcct gtagggcgaa gggaccgtga gagacgcaac tgaacaacgc
    30661 gccccagaca cggcaatttt tatcttcgaa atagaactgc ccgtgctcgg caaaggggac
    30721 accggcaatg cccaactctt cttccgcttc gcgacgcgcg gattccagca gttgctcatc
    30781 ggcctgaact acaccgcctg cggtcgcatc taacatgccg ggtaaaaagt cttttgtctc
    30841 ggtacgacgc tggaccagaa ttttgcccat gccatcatgc acgacgatgt aagttgcacg
    30901 atgacgcaga cactgtgccc gcatttgttc ccggctggct tgtgcaatga cttcgttctc
    30961 ttcattgaca atatccaccc attcagtact tgccaaacga cgctgttcca ttatcaggaa
    31021 accttctttt tctggcgctc ttacggcgca ttttgagttg tgggtaaatt acggattaat
    31081 cgcgacctgc gcaatgatac tttgatcatt gagtgcgatt acactaagta cgtcattatc
    31141 cagcatgcca taacccgccg gattaccgcc tttcggaata ctcaccgagc cggggttgaa
    31201 gtggaaaatc tccccccgct gttctgccac tggcagatgg gtatgaccgt acaccagcac
    31261 atcgttctgg tttaaagcag gtagattttc cgggccaaaa agatgaccat gcgtcaaaaa
    31321 cagacgttgt ttttccagta atacctgttg ccacggcgcg gttatcggga aatgcagcat
    31381 catttgatcc acttcgctgt cgcagttgcc acgcacagcg ataaccttat gtgccacttc
    31441 attaagccgt tcagcgactt tggctggcgc gtaaccctcc ggtaaagcat tacgcgggcc
    31501 gtgattcaac acgtcgccaa ggatcaccag ccactgggca ccgctttgga caaacaactc
    31561 cacaacacgt tccgtcgccg gtaacgaccc atgaatgtcc gatgcaaaca tcagtttcat
    31621 cactcactcc tcgtcgaaaa aacgtaagct atgatactgg attctgtcgt tgctatcagc
    31681 cagattgctt cgccgaaagt gcaataaaac gctggacaga cgctcgctgc cactggaatg
    31741 tcgcataatc caccaggcgt tctggcacct catcgccatg tagtaccagg cggttaatca
    31801 ttagcgccag atcagtatca gcaatgcacc attcaccaaa taaattcggc tgacccagtg
    31861 ctaacaaatg ttctgccatc gcgaacagtt tctctgcact ggcttttccc tcggccgtta
    31921 gtggcgcttt tttcgccccc gcaaagacaa catccgtcgg acgctcttcg cggatgggca
    31981 tcagatcgct gcgcagccag gcctgaatct gtcgtgcacg cgcacgattt tctaaatcaa
    32041 gcggataaat acgttcccag gtcggtggcg caaatcgatc ttccagatac tccgcaatgg
    32101 cagaagattc actcaactca aaatcatcga tttgtaataa cggcacacgg cgtgtctgac
    32161 cgtaaccttg ccacgtcggc tgcaaatgtt caccgctgtc gaggtcgatg gtcttgatat
    32221 gaaacgacag gcctttttcc tgcaacgcca cccaggcgga taacacataa ggggagaaaa
    32281 agtgggcatc tgaccaaagc gtgatagcgg gtttactcat tatgtcctcg gcaataaagg
    32341 gggctggcta tcaaactata gagccttttt gtcactgtca cgtgacgaat actcacgtaa
    32401 gtcgccgcgc tttctatact tgtggtgttc agatgattaa acaaccggag ctgcaatgat
    32461 cgatctctat ttcgccccga cacccaatgg tcacaaaatt acgctgtttc tcgaagaagc
    32521 agagcttgat tatcgcttga ttaaggtaga cttggggaaa ggcggtcagt ttcgtccgga
    32581 atttttgcgc atttcgccca acaacaaaat tccggcaatt gttgatcatt ctcctgccga
    32641 tggcggcgaa ccgttaagcc tctttgagtc tgctgccatt ttgttgtatc tggctgagaa
    32701 aacaggactc tttttgagtc atgaaacgcg tgagcgcgcc gccacattac agtggttatt
    32761 ctggcaggta ggcggactgg ggccgatgct tgggcaaaat catcatttta atcacgcagc
    32821 cccccaaacc attccttacg ctattgaacg ttatcaggtt gaaactcagc gtctttacca
    32881 tgtactaaac aagcggctgg aaaactcgcc ctggctggga ggcgagaact acagcattgc
    32941 ggatattgcc tgctggccgt gggttaatgc ctggactcgc ctgcgaattg acctcgcaat
    33001 gtatccggca gtcaagaact ggcatgagcg gatccgttcg cgccctgcca ccgggcaggc
    33061 actgctaaaa gcacaactcg gtgatgagcg ttcggatagt taacagaaac aggttctcgt
    33121 gtattatttc atcctaagta aaacaacgga gaacctgcaa tggcacaacc tgccgctatt
    33181 attcgtataa agaaccttcg tttgcgtacg tttatcggaa ttaaggaaga agaaattaac
    33241 aaccgtcagg atattgttat caatgtgacg atccactacc ccgccgataa agcgcgcact
    33301 agcgaagata tcaacgatgc gctgaattat cgcaccgtaa cgaaaaacat tattcagcat
    33361 gtagagaata accgtttctc tttgctggaa aaattaactc aggatgtgct cgatatcgca
    33421 cgtgaacatc actgggtgac gtatgctgaa gtggagatcg ataaactgca cgcgctgcgc
    33481 tacgccgatt cggtatccat gaccttaagc tggcagcgtt aatcgccata tcgggaggct
    33541 gcatgaacat agtgatcacc ggagggacgg gattaattgg tcgccatttg attccgcgtt
    33601 tgctggagct gggccatcaa attacggtag tgacgcgtaa cccgcagaaa gccagttccg
    33661 ttctcggccc tcgggtgaca ctatggcaag ggcttgccga tcaaagcaac ctcaacggcg
    33721 ttgatgcggt aatcaacctg gccggagaac cgattgctga taaacgctgg actcacaagc
    33781 aaaaagagcg tctctgccaa agccgctgga atatcacgca aaaactggtc gatttgatta
    33841 atgctagcga cacgccgccg tcggtactca tttccggctc ggcaacgggc tattatggcg
    33901 acttaggtga agtggtggtt accgaagagg aaccgccgca taacgaattt actcataaac
    33961 tctgcgcccg ctgggaagag attgcctgcc gggcgcaaag tgacaaaacg cgagtgtgcc
    34021 tgctgcgaac cggcgtggta ctggcaccgg acggcggtat tctcggtaaa atgctgccac
    34081 cgtttcgtct tggcctgggc gggccgattg gttccggtcg gcagtatctg gcctggattc
    34141 atattgatga tatggtcaac ggcattctct ggctgctgga taacgagctg cgcgggccat
    34201 ttaatatggt ttcgccctac ccggtacgca atgaacaatt tgcccatgcg ctcggtcatg
    34261 cgctgcatcg cccggctatt ttgcgcgtcc ccacaaccgc cattcggctg ttaatgggcg
    34321 aatcttcagt actggtatta ggtggacaac gcgcgctgcc taaacggctg gaagaagcgg
    34381 gttttgcgtt tcgctggtac gatttagaag aggcgctggc ggatgtcgtt cgctgatgtg
    34441 gtttacagca aacatccgcc agttaactcc ctgtgttaca ggattagtgg ctttgcgcga
    34501 taagatcgtc tggtgaaagt cgggtcacca tcataactaa ctctctgtct aaacctctat
    34561 ccagcatctc ctgagcaata cgcagggctt cttcgtgttt gccctgcatt gcgccttctt
    34621 cacgtaatct gtcagcaatg gtcatcagtt tctccttttc ttgtggtgcg cgttctgtta
    34681 tctcaccaat aaatgcacgg aaacgctggg catcccctgt ttgtaatacg taattaaaca
    34741 gggcttttag ctgtctgtca ttagtgttcc ctgtaactag cagcgaaaca atttggtcga
    34801 ctaatcccaa cagatcgcgc tgacgaatat gtttctgaat taactccaac agcgccattt
    34861 tgcggtgttg cataatctcg tcatccggca ccacggtaat atccaccaac ggaaacgctg
    34921 atgaatatat tttgcgggct atagcaggct cggcaaattc atcaagccag cagagtgaat
    34981 aaggataagg acttctgcaa ccatgataaa acagcatcgg gatcaccaat ggaagctctt
    35041 tatagcccgc atcaagatgg ttttgcattg ccgcaatgga ataacgcatc atgcgaaaag
    35101 ccattaattc ttccggctta ctttggtgct ctatcactac ataaatataa cccactccct
    35161 cctgcgtttt cacagaccac aagaggtcgg aataatattg ccgcaggtct tcatcaataa
    35221 aactgtttgg ttctagttta agcgtcgtta aatcacacag tttgcgcagc ggcgcgggaa
    35281 gatgaatatt aataaaattc cgcgcggtgt ctggatggcg taaaaaagat ttaaataccg
    35341 catcatgcgg cgtggaagtt gtcgatattg tcatggcgtt ccgtcacccc tcaaatttaa
    35401 gatgacgcga caataaccac gattaacacc caatgcagca ggcagttttt atctttggac
    35461 agcgaattca gaagtatttt tagcggactg caacttttac atttcagcgg aagctgactc
    35521 gcaaattccc cttgaaaata ctccggcagc gttagccacc ggagaggaaa ttatttcagc
    35581 gatcccttaa ggaactgttg cagacgaggg ctttgcgggt tgccaaataa ctgctccggc
    35641 gcgccctctt cttctatttt cccctgatgg aggaaaatga cgtgagtaga aacatggcga
    35701 gcaaagccca tttcgtgagt cactaccacc atggttttcc cctcttctgc cagttgctgc
    35761 ataatacgta acacttcgcc taccagttca ggatcgagcg ccgaggtagg ttcatcaaac
    35821 agtaaaactt ctggttccat cgccagcgcc cgcgcgatag aaacacgctg ttgctgaccg
    35881 ccggaaagat gcaccggata tttcccctgc gcacgttcgt ctatcccgac tttcgccaga
    35941 tacttcaccg cccgctcgcg cgcttcctgc ttgctcaggc ccaacacctg aatcggcgct
    36001 tccatgacgt tttccaacac cgtcatatgg ctccagagat tgaagtgctg gaataccatc
    36061 gtcaggcgtg tgcgcagtaa gcgcagttga tttttatcgg cgactttgag ttgaccgtct
    36121 ttgtcgcgca ccagattgat cgtctggcca ttgaccacga tcgatccttc actcggtttt
    36181 tcgaggaagt taatgcagcg cagaaaggta cttttccccg atcccgacga tccgatgatg
    36241 cttattacat ctccggcatt cgcttgcagt gagacccctt tcagcacttc gtgttcgcca
    36301 tagcgtttgt gcaaatcgat aacgtttaat ttattctcgg acatcgtatt ctcagtgcgt
    36361 tgaagaaggt ttcacatgct gcaaccagcg tttttccgct ctgcgaaaga ggctgatcag
    36421 gacataagag atgattaaat agagcaccgc ggcaatgccg aaggcggtaa aaggttgata
    36481 cgtggcggcg ttaatatcgc gggctatttt cagcagatcc ggcacagtgg ccgtaaacgc
    36541 cagcgcagta gagtgcagca tcaggatcac ttcgttgctg tatgccggta acgcaatacg
    36601 cagcgcagaa ggcaaaataa tgcagcgata cattttaaag gtcgagaagc cataggctct
    36661 ggcggcttca atttccccat gcggaaccga acggattgcc ccagcaaaaa tctcggtagt
    36721 gtaagcgcag gtgttaagcg tcagcgccag cacggtacag ttcaggccac tgcggaagaa
    36781 agcgttaagg aattcggttc ccttaacaat ctcaagcgtg tacatgccgg aatagaacac
    36841 cagcaactga acatacagcg gcgtaccgcg aaaaatatag gtaaataacc agattggaaa
    36901 ctggatgtat ttattactgg agacacgacc aatcgccaga aacagcgcca ggactccgcc
    36961 tatcactacc gacaaaataa gcagccacag agtgatcgcc acaccagtaa agcgataacc
    37021 gtcggtccac agcagcggtt tccagtattc atgtaagatt tcgatcacag gtcagccctc
    37081 ttcacaccca cggagtagcg gcgctcaagg aacagcagca caccattgga aacggtggtg
    37141 aaaaccaggt aaatcacgcc acagacgatg gcgaaataga acggttccca ggtacttttg
    37201 cctgccagct gcgtggcttt gaccacatct tccaggccga gtaacgaaac cagtgcggta
    37261 gatttgagga tcacctgcca gttgttgcca atgcctggca gggcgtaacg catcatcgcc
    37321 ggaaacatga tccgccgaaa cacttgcccg cgagtaaaac cgaacgccgt cgccgcctct
    37381 atatgccctt tcggcactgc cataaacgcg ccacggaacg tttcggtaaa ataagcaccg
    37441 tagataaaac cgagagtgat aataccagcg accatcggat cgatatcaat ctgcccgacg
    37501 cccatcgcct ccgtcaccat gtttagcgca atctgcaaac catagaaaat cagcagcatc
    37561 aacaccaagt ccggcacgcc tcgaatcagc gtggtgtacc cttcgaaaat cagccccgaa
    37621 agccgatttt gcgagagctt accgccagcg ccaattaaac cgatgattac agcgagcact
    37681 acagagctga tagccagctc cagcgtgacg agcgcaccct gtaaaataac acctgaaaac
    37741 ccatacaaca tgctgcctgt cctgtcgtgt gtggtgaatt accgcctctt gtctccctcc
    37801 ggttacccga agggagcggg ctttctttca ccgatggcga ttaaccacca taaacatcaa
    37861 aatcgaagta ctttttcgct aatttctcgt aagtaccgtc agcgcgcatt tcggcaaagg
    37921 ctttgttcag tgcctcgcgc agttcgttat cttctttacg caggcccatg ccggtcccta
    37981 cgccaaacag tttttcatct ttaacagacg gaccaccgaa tttgtaatct ttaccgacgg
    38041 gttgtttgag gaaaccttcg ctggcagcga cctcatcctg gaacgcggca tcaatacgtc
    38101 cggcagtcag gtcagaataa atgttgtcct gcccctgata cgagacgatt tcaatgcctt
    38161 ttggtgccca gtgttcatta ccgaacgtct cctgggtggt gccctgcaat acgcctaccc
    38221 gtttgccttt cagcgactcg actgtcggct gaatgtcaga atttttcgcc accaccagac
    38281 gagaatcggc agcgtacagt ttgtcggtga aggcaatttc ttgctgacgt ttttccgtaa
    38341 tggaaagcga tgacatgatg gcgtcaatct tcttcgcttt taaggacggg attaacgcat
    38401 ccagcgggtt ttcgacaaac gtacattgcg tattgatgcg cttgcacaac tccttcgcca
    38461 gctcgatatc gaaaccaacc agttcgcctt gtgaattttt tgattcaaat ggcgcatagg
    38521 tcgggtcggt accgatgcgg atgttttgcg gaattgcagc aagtgccgca gttgcgctgg
    38581 agaaggccag aaccagagag agcgatagca ccagtttttt cataatgtcc tcaactgaca
    38641 gtctttttat agggaatatt tacaggtttg ttacacttat cgtgccataa aattgaccat
    38701 caaggcaaaa tatcccgccc cgttagacgt attatttttc attaaaaatg cttaagtatg
    38761 catttcaatg caccacgata gtgcaacaaa cctggcgtgc accatgatga tcattccatc
    38821 aggtacagct tcccagcgac gtatcagtca ccgtagacat taaagtcgaa atactttttc
    38881 gccatcttgt cgtaggtgcc gtcctgacgc agctcgccaa gcgccttatt gaaggcagcc
    38941 gtcagttcag cataatcttt acgtagccct acaccggtgc catcaccgaa gtattttttg
    39001 tcttttactg atgagccagc aaaagcgaaa tctttaccag caggttgctt gaggaatcct
    39061 tcgctggcag caacttcatc ttgtaacgca gcatccagac gtcctgcagc cagatcggaa
    39121 tagaccaaat cctggttggc ataggccacc acatccacgc ctttactacg ccaggtctcg
    39181 ttagcgtaag cttcctgggt tgatccctgc agcacaccaa catgtttacc tttcagtgaa
    39241 tccagcgttg gctgaatcgg tgaacctttg gcagcaatca aacgagaatc tgcggcgtac
    39301 agcttgtcgg agaaggcaat ctcctgctga cgtttatcgg taatggaaag cgacgaaata
    39361 atagcgtcga tttttttcgc tttcagtgag gggatcagcg cgtcaaagtc actggcaacc
    39421 caggtacatt tcacctgcat ccgtttgcac atctcgttac cgagatcgat atcaaagcca
    39481 acaaaatcac ctttagcatc tttcgacgag aacagcgcat aggtggtatc ggttccgata
    39541 cgtaccgtct ccggtagcgc cgcatagctg gaagccgctg cggagagacc gactaataaa
    39601 gacagagcga gaatcgactt cttcatacat aaccctcaag tgaaaatggc ttttttatgt
    39661 tgtgtattgt gttgtgtgtt tgcaggctct ttcatgcagg tcttatgcca tcttgacgac
    39721 aacagtaaca ttcaacgtta aatatgttaa taagacattg cattattgtc ctgaagttga
    39781 agatagcagg tatagcggtt gaatcgcagc gtttcgattg aatggcagaa acaaaatgtc
    39841 gaggatttga tcgcggttgc aaaattgccc tgaaacaggg caacagcgga gttatgcgcc
    39901 ctgccagcgg gcaaagagat cttcaggaag ggttattgca aactggtcaa gaacacgatt
    39961 aaccgtctga tttatcacat catcaaggga ttgcgggcga tgataaaacg ccggaacggg
    40021 aggcataatc accgcaccga tttctgccgc ctgagtcatt aaacgcagat ggcctaagtg
    40081 caatggtgtt tcacgcacgc agagcaccaa cgggcgacgc tctttcagca ccacatctgc
    40141 cgcacgggtc agtaagccat cagtatagct atggacaatg ccggacaggg ttttgattga
    40201 acagggtaaa atcaccatcc ccagcgtctg gaaagaaccg gaagagatgc tggcggaaat
    40261 atcgcgcgca tcgagcgtga catcagctaa tgcctgcact tcgcgcaggg aaaaatccgt
    40321 ttcgagggat aaggtctggc gcgccgcctg gctcatcacc agatgcgttt cgatatctgt
    40381 gacatcgcgc agaacctgta ataagcgcac gccataaatc gcgccgctgg caccgctgat
    40441 gcctacaatg agtcgtttca taaatagttg ccctgtcaga cttgcaggca gactttgcag
    40501 tatttcggcg ggagttgcaa gtcagggtgc cagaccggca ccctcagcga aggcatcatc
    40561 cttcgttatg catttcgaga ttttccactt cgttctgacg ttgcactgct ttggcgtcat
    40621 cattacgtaa cgtatcgagg aaatcgaggt agccctgatc aacatctttg gtgacgtaga
    40681 cgccgttgaa caccgagcat tcaaactgct ggatatccgg attttcagcg cgaacggcgt
    40741 cgatcagatc gttcagatcc tggaaaatca acccgtcagc accgatgatc tggcgaattt
    40801 catcaacttc gcgaccgtga gcgatcagtt ccgtggcgct cggcatatca ataccataaa
    40861 cgttcgggaa gcgaatttcc ggtgccgcag aagcgaggta cactttcttc gctccggctt
    40921 cgcgtgccat ctcgataatc tgctcagaag tggtgccacg gacgatggag tcgtcgacca
    40981 gcaggacgtt tttatcgcgg aactcggcgc ggttggcatt cagtttacgg cgcacggact
    41041 tacgacgcag ctgctggccc ggcatgataa aggtgcggcc aacatagcgg tttttaacga
    41101 agccctggcg gtacggtttg cccagaatac gagcaatttc cagcgcgata tcacacgagg
    41161 tttctgggat cgggatcacc acgtcgatat ccagatcttc ccattcgcgg gcaattttct
    41221 cgcccagttt cgtgcccata ttcacacgcg cgctgtaaac ggaaattttg tcgataaacg
    41281 agtccgggcg ggcaaagtat acatactcaa acaggcacgg attgctgacc ggattgtcag
    41341 cacattgacg ggtaaacaac tgcccttctt cagtgatgta aatcgcttcg cccggcgcga
    41401 cgtcacgcag gaaatcaaag cccagcgtat cgagcgctac gctttcggaa gcgaccatat
    41461 attctgtacg gttctcgtca atatcacgtt ttcccagtac cagcggacga atcccgtttg
    41521 gatcgcggaa agcaaccata ccgtggccga taatcatcgc cacacaggca tacgcgccgc
    41581 ggattaagcg gtttgtggca gcaatggcag cgaaaatatt gtcggcttcc agcgggtagt
    41641 ggcggaagtt gtccagctcg ctggcgaaga tattaagcag aatttccgag tcggaagtgg
    41701 tgttgatgtg gcggcgtttt tcttcaaaca gttttttacg caactcgtga gcgttggtca
    41761 gattgccgtt gtgggcaagc gtaatgccat acggggagtt aacgtaaaac ggctgcgctt
    41821 cagaggcgct ggagctgcca gccgtagggt aacgcacatg accaatgccc atattgccct
    41881 gcaaacgctg catatggcga gcttcaaata catcgctcac cagcccgttc gctttacgca
    41941 aacggaagca gttattggca tctatggtga tgatgccggc ggcatcctga ccgcgatgct
    42001 gaagcaccgt taaggcatca taaatcgact ggttaaccgg cataacaccg gcgataccga
    42061 caataccgca catacgtctt tttcctcgtt aagccacatc tcagagcact tacgctctgg
    42121 gcaagaaact tgacgagctt tgcagataat caaaaaacca tctgatgata aaactgaatt
    42181 gcgggatcag ctgtgatttg ctccagtctt cacttttcga caccccggta aaggagtcga
    42241 gaaagaagag aatggcggca acaatcaaca caccgcgcaa cgcaccgaaa cagacgccca
    42301 gcacccgatc ggtgcctgac aaccccgttt tctccaccaa ctggcctatc acgaagttca
    42361 cgatagcacc aacgatcagg gtagcgataa acagtaccgc gatggcaatc ccatttcgaa
    42421 ccagttcgtc ttcaaagccc gtaaaccaga ctgacaggta agtgtagtaa tgactggcaa
    42481 caaagaaagc acaaccccat gtcaccagcg ataacgcttc acgaacaaag ccgtggatca
    42541 ggctaaccag agaggaaaaa gcaatcaccg cgattatggc gtaatcaatc cagaccatat
    42601 gtgtcccacg attttacgcc ctgtcatcct gttcggggcg cattctaaca gaaaaagaaa
    42661 acgtttgcgt agggatttcc ttcccgcgca tcaataaaaa tggcgctgaa aaaatattca
    42721 acgccatcga ctttttatgc ctttgcggca tcgtgcaatg cgtgtcggat gcggcgtaaa
    42781 cgccttatcc gacctacggt tctaacactg cccaagcctg ataagacgcg ccagcgtcgc
    42841 atcaggcaag actgtattaa ttcggcgtat agcccattac cacgccactt aagccagaaa
    42901 gttgcttcaa ctcacccagc gaacctttca gcttatcttt cgaggcatcc ggcccaacca
    42961 gaatacgggt aattttaccc tgcactggcg tggatggcga cgtataaacc cgataaccgg
    43021 caccgcgcag cttaccgaca atctcattca ctttatcggc atttttcagc gcacccagtt
    43081 gcacaacata ggctttaccc gttggcgctg ctttttcttc cacgaccggc ttcggttctg
    43141 gcttcggcgc aggtggtact tcaacctttg gtttaggcgg ctccaccggt ttcggcttcg
    43201 gtggggcgac cggtgcaggt tccggttcaa actcggtgtt attggctgca atagtggccg
    43261 gatcgagcga cggtgctgcg gcatcacctg cccgcacctc ttccgctgcg ccttccggcg
    43321 gctgcgtcgg caacgcctgg gtggcggctg gcatcatatc aggctcatca cgatcgcccg
    43381 ctttcggcac cagcgggata gccgcgaact catcctgata atgttttttc tgcccgtcca
    43441 gcagccctgg aagtacaatc acccccagcg ccaccagcac gatcgtgccc actaaccgat
    43501 tctgaaactt acttgccacc gcttctcctc gcgtcaatca cttccatgac atgtgcgacc
    43561 gtgtggaaag aaccacacac cagcacggtg tcttccgctt tagcgtccgc cattgcggca
    43621 tcccatgcct gcgcaacgct atcaaatgat ttgccgttac ccaaatgctc aagcagttgt
    43681 tctgccgtgg caccgcgcgg cccttccagt ggcgcacaat accagtcatc aaccacgctt
    43741 ttcaaccagg ccagagttcc ggcaatatct ttatcatgta gcataccgat aaccgccagc
    43801 acgcgcccgt ttttcggtag cgctttcata cgcccggtga gatattccgc cgcatgtgga
    43861 ttatgcgcga catcaaaaat aacgcgtggc gactcgctca caatctggaa acgtcccggc
    43921 aaaattgcgc tggcaatccc gtcgcgaatg gcattttcac tgacttccag cccgctggca
    43981 cgcagtgccg ccagcgccgt tgcggcattc ggttgcggga caagcggcaa cggcagattt
    44041 tccagcgtgc cgtgagcatc gctaaacgcc cagtcatgat cggtgacgga atagttccac
    44101 tcaacgcccc gacgttgtaa cagtgcacct ttttcctgcg ccacatcagc aatggtagaa
    44161 ggcatttccg gctcaccgac aattgccggt ttttcgctgc ggaagatgcc tgctttctcg
    44221 agaccaatac tttcgcgatc tggacccagc cagtcggtat gatccagcgc aatactggtt
    44281 actaccgcga catcggcatc gacaatattg gttgcgtcca gacgaccgcc cagccctact
    44341 tccagaatca ccacgtcaag ttgtgcctgc ttgaacagcc acaacgccga cagcgtaccg
    44401 tactcgaaat aggtcaggga aatatcaccg cgtgccgatt caatctccgc aaaagaggcg
    44461 gtgtgggccg attccggcaa ttcctggccc tgcacacgta cgcgctcggt ataacgcacc
    44521 agatgaggcg aactgtagac gcccactttg taccctgccg ccatcagaat cgactccagc
    44581 gtacggcagg tggtgccttt gccattcgta cccgcaacgg taaacacaaa tggcgctggt
    44641 ttcaggacgc caagacgcgc cgcgacctgg ctcacgcgct caaggccgag atcgatagtt
    44701 ttactgtgca ggttttccag ataagaaagc cacgaagcca gaggcgacgc ggcttgagga
    44761 gtgcgtttga taatcatggt atccgctgat tcgttacggt gagaatagca aaagggcaga
    44821 gccagtggcc ctgcccttat cagttatcag gcctcaggtt cctgatccgg taccgggggt
    44881 accactacgc cttcacgcgg cgcttcagga ttcggcgctg gcagattcat caacttcgcc
    44941 agaatgctcg ccagtttcag gcgcatttcc ggacgacgga cgatcatgtc gatcgcgcct
    45001 ttctcgatca ggaattcact gcgctggaat ctaggcggca gtttttcgcg aacggtctgt
    45061 tcgataacac gcggaccggc aaagccgatt aacgctttag gttcagcgat gttgagatcg
    45121 cccagcatgg cgaaacttgc agaaacaccg cccatcgtcg ggtcggtcag cacggagatg
    45181 tacggcaagc cgcgctcctg catttttgcc agtgccgcag aggttttcgc catctgcatc
    45241 agcgacatca gtgcttcctg catacgtgcg ccaccagagg cggagaagca gatcagcggg
    45301 cagttatctt cctgcgcctg ctcaacggca cgcacgaaac gtgcacccac aacagacccc
    45361 attgaaccgc ccataaaggc gaactcgaat gccgcagcga caaccggcat tccatacaga
    45421 gtgcctttca tcaccaccag cgcatctttt tcgccggttt ctttctgcgc agatgccaga
    45481 cggtctttat acttcttgga gtcacgaaac ttcagctcat ctttcggctc aagctcgcta
    45541 cccagctcca caaggcttcc ttcatctaac aggctatgca ggcgattacg cgctgtcata
    45601 cgcatgtgat ggtcacactt cggacagacc tcaagattac gttccagctc agcgcggtat
    45661 aaaacctgac cgcagctatc acacttagtc cacacccctt caggaatgct cgccttgcgg
    45721 gtgggagtaa tgttgctttt aattcgttca atccagctca ttagggacct ttctgtctga
    45781 acctggttcg atgccagttt tatctttggg gacgcataat gccatttttg cccccaacag
    45841 accatgaatg ttgcacatta aaacataaca gcccgaaact ttggataaaa aagtggtcga
    45901 accgcggagt tactttttat tttgcggcgc gtgccgcagc gcgtttgtga cggattattt
    45961 cgatgacgcc cggcaaaata gaaaccacaa taatcccgac gatcagcagc ttaaggttat
    46021 cctgaaccat cgggattgta ccgaagaaat agcctgcgta ggtaaaaagc agtacccaca
    46081 acagtgcgcc gatcacgtta taagcagcga aatgacggta cgacatgtgg cccattcccg
    46141 caacaaacgg ggcgaacgtt ctgacgatcg gcacaaaacg ggcgagaata atcgttttgc
    46201 cgccatgttt ctcataaaac tgatgggttt tgtcgagata actgcgacgg aaaattttcg
    46261 aattgggatt actgaataac ttttcaccga acaaccgccc gatagtgtaa ttgaccgcgt
    46321 cacccacaat cgcggcgatc agcatcagca ctaccatcat atggacgttg agatcgttgg
    46381 tttccagtga tgccaacgct ccggcaacaa acagcaatga atcacccggt aaaaacggca
    46441 ttaccaccag accggtttca cagaacaaaa ttaagaacaa aatggcataa acccagacgc
    46501 cgtactccgc gactagctct gccagatgca cgtcaatgtg caggataaaa tcgatgagga
    46561 aataaatcag gtccatattt gcctatgcct tgtactcgtc atctttcagg ctgtaactac
    46621 gttggctaca gcctgaaatg ttccgagcat tattctcgtt agtccgccag aaatagcggg
    46681 cccattggcg gttttggaag atcataccgg tcagggtaat ccaccgcgac cagatacagc
    46741 ccttccgctt ttgccgttgc tgccgccagc gttctgtcct ttgccgccag caactctgct
    46801 atccagctct ccggctggtt gtgggcaccg acttccatca ggctgccgac aatattcctg
    46861 accatatgat gtacaaaggc attcgctttg atatcaacca ccacataagg accgtgacgc
    46921 gtgacattaa tatgcataac gttgcgccac ggggttcggg actggcactg caccgcacgg
    46981 aacgaggtga aatcattctc gcccagcaag cattgcgcag cccgatgcat ccgttcagcg
    47041 tccagcggtt cgtaaaaatg ggttacccct ttactcagta ccgccgggcg cagccgatga
    47101 ttgtagatga tgtagcgata acggcgagcc gtggcgctaa atcgggcatg aaaatcatca
    47161 ggtacagctt taacccaacg cacggcgatg tcaccaggta aattcgcatt tacgcccaag
    47221 gtccacgccg cgtctttgcg ctgcgcggtt gtttcgaaat gcaccacttg cccggtaccg
    47281 tgtacccctg cgtcagtacg cccggcgcag aagacggtga tgggttcgtt cgccacctgg
    47341 gagagcgcct tttccagctt ctcctgcaca ctgcggactt cattctgccg ttgccagccg
    47401 taatacttac tgccgtcgta ctcaatgccc agcgcaattt tataaactgg cagttgttgc
    47461 tggtcggaca ttagtacaga tactcctgca ccagtttctc ggcgattttt actgccatca
    47521 gcgcgccgcc aaagcgaacg ttatcggcca ccgaccagaa ctggacttgc tccggcatac
    47581 cgtagtcatt acgcacgcag ccaacagaaa gatgcggcgt acccgaagca tcccctacct
    47641 gagtcgggaa ttcgttctct tcagagagca caatatcttc gccctgagca aacgcatcac
    47701 gcgcttcttc tgccgccagc ggacgcagtg cttcaaagtt gaccatctgg gcatgaccgt
    47761 agaataccgg tgcctggacg acgcttgccg aaatcatcag cccttcgtcc tgcaggattt
    47821 tgcgtacttc gtcaacgata cgacgttctt cacgcacgct accttcgcta tccggcaaca
    47881 acggcagcat attgaacgcc agttgacgac cgaagaaatc ttcttcgtca atcggaatgc
    47941 cgttgagcaa tttcgcactc tgccccgcta acgcatcgac cgcttttttg ccctgggcgg
    48001 aggctgaaat caggctggta acactgatac gcgacaaacc gccctgatcg attaacggtt
    48061 tcagtgccgc cagcagctgg ctggtcagac tgtctggtac ggcgatgaca ttccggttgc
    48121 ggtaatcggt cagtacaaac gggtttactt ccggcaccac cagcggtacg tcgggttcga
    48181 gagcaaacag tccgctgctg tcgatcacca ggcaacctga gttggtcgct tcttcaaccc
    48241 aggcagcggt agcttctttg cctgcgacaa aaaatgccag ctgcgcctgc gtccagtcga
    48301 attcagcggc atcctgcacg gtgattgttt taccaccaaa gcgcagttgt tcgcctgcgc
    48361 tttcgttacg tgccagtgca taaatttccc caaccgggaa ctgacgttca gccagcgttt
    48421 caagcagggc ttcgcccata gcgccagttg cgcccaggac ggcaatgttc cagccttcag
    48481 acatggtggt ttactccaga aatagcaaag ctccctgcca atgttacagc agagagcatg
    48541 aagaagagat taacgtgccg gatgatgaac ggcgttaaaa cccagtttac acagcaatga
    48601 tgccgcactg gcgtcatcac aaattacata cagagatgac cattcacggc gctcaagata
    48661 gtttttgcgc agtttatcga actcacccgg tatcccggcg actttacgca gcggtgcgtc
    48721 atcgcggcgc acatcataca ccaaatgcac cagccttttc agcgtcggtt gatcgagcgg
    48781 accatgcagc gtaatgcggc caaactcagg cgcgggcagc aacgtatcca gcgcaacgtg
    48841 ttgttcatgc ccaataaact tgctataggc ttcaaacact tgcgtcgtgc cacgcgcttt
    48901 accctccagg gtatagcctg cgatatgcgg cgtgccgata tccacttttg tcagcaattc
    48961 cacattaagt tccggctcgc cttcccagac atccagcacc acgcttaact tctggccttc
    49021 attcaggcag gtcagcagcg cggtattatc gacgactgcg ccacggcagg cgttaatcag
    49081 aatcgctccg ggcttcaggc tacggatcag tttttcatcc gccagatgta gcgttttgta
    49141 cggaccatct ttaaagagtg gcgtatggaa agtcagaata tccgcgcgct ggactaactc
    49201 atccagcgag cggaaatcac cctcatcccc acggtcggcg cgaggcggat cgcaaagtaa
    49261 ggttttaatc cctaacgctt ccagtcgcgc ctgcaatcga cgtccaacgt taccaacgcc
    49321 cacgatcccc accgtacggt cgtgcagtga aaatccatcg cgttcggcaa gcatcagcag
    49381 ggaggagaaa acatattcca ccaccgcaat cgcattacag ccaggtgcag cggaaaaacc
    49441 aattcccgcc tgcttcaacc atgcttcatc gacatggtcg gtccccgcag tggcagttcc
    49501 aacaaattta atgggttttc ctgccagcaa agattcattc actttcgtga ccgaacgcac
    49561 catcagcgca tccgcgtctg ccagttgagc gacggggatt ggacgcccgg gaaccgcgat
    49621 cacctcaccc aaacggctaa ataagtcgcg ggcataaggc atattttcat caacaaggat
    49681 tttcacgttt gtgttacctg tatgagacga gagttaaccg gacaagtatg ccataatctc
    49741 gcggccaggc atacttgcga agatttcagg tataaggata cgtaatgata caacctattt
    49801 ccggccctcc tcctgggcag ccaccaggtc agggagataa cctgccgtct ggcgcgggca
    49861 atcagccttt atccagtcag caacgtactt cgctggaaag cttaatgacg aaagtgacct
    49921 cactgacgca acaacaaaga gcagaactgt gggcgggtat caggcacgat attggtctgt
    49981 cgggagattc accgctgctt tcgcgtcact tccctgccgc tgagcataat ctggcgcaac
    50041 gtctgctggc cgcgcaaaaa agccattctg cccgccagct tttagcgcaa ttaggggagt
    50101 atttacgtct ggggaatcat cgtcaggcgg tcacggatta tatccgtcat aactttggtc
    50161 agacgccgct gaatcagctc tcaccggagc aattaaaaac cattctcacc ctgttgcagg
    50221 aagggaagat ggttattccg caaccacagc agcgcgaggc gaccgaccgt cctttattac
    50281 cggcggagca caatgcgcta aaacagctgg tgaccaaact tgcggcggca acgggggaac
    50341 ccagcaaaca gatctggcaa tcgatgctgg aactttccgg ggtgaaagat ggcgagttaa
    50401 ttccagcgaa actgtttaac catctggtga cctggctaca ggcgcgtcag acgctaagcc
    50461 agcaaaatac gccgacgctg gaatcactac agatggcgct aaaacaacct ttagatgcca
    50521 gtgaactggc ggcgttatcg gcatatatcc agcaaaaata tggcctttct gcgcaatcat
    50581 cgctttcttc tgcccaggcc gaggatattc ttaatcagct ttatcaacgg cgggttaaag
    50641 ggattgatcc gcgtgatatg caaccgctgc ttaatccttt tccaccgatg atggacacgt
    50701 tgcaaaatat ggcaacgcgt cccgcgctgt ggatactgtt agtcgcgatt atcctgatgc
    50761 tggtctggct ggttcgttaa ccccgacgaa atgacagtat cgtgacaata atacccagca
    50821 ccgcagagat cgccccggca agaaataccg aagagtaacc aaacgtggtc gccagcattc
    50881 ccgccagcgg cccggagacg ccgagggcga tatcctgaaa cgcggcgtaa ccgcccagtg
    50941 cggtgccgcg aacttgtgag gggacgcgtt taaccacctc cacgcccagc gcaggaaaga
    51001 taagcgaaca tccggctccg gttaacgccg cgcccgctaa tgcgacccat gcacctgggg
    51061 cttgccagag cagcaacaag cccaccgttt ctacaagcag agagacaatc gccactttca
    51121 cgccgccaaa acggtccggc atccagccaa acatgacgcg catcacgaca aatgcgccgc
    51181 caaacgcggt aagagtaaag cccgccatcg cccatccttt gctggcaaag tagagcgaaa
    51241 cgaaagtccc gataaccgca aaaccaacgc cttgtagtgc cagccctaac cctggtttcc
    51301 agataagccc gacaacgctc cacagcgatg gacgttctcc cgccagggcc ggtactttgc
    51361 gcactgtgcc gttacaggcc cacgccagta agggtaatac cattgtggtg atcgccagtg
    51421 cggcaaaacc gtaatggcta tgaatcaaca ggccaagcgg cgcaccaaca gcgagggcac
    51481 cgtaaatcgc cattccgttc catgacatca ctttgccaga gtgttttggc cctacgatgc
    51541 ctaaccccca ggtcagagcg cctgtcagta actggctttc accaaaccca agaatcaaac
    51601 gcccgatgac caacagggca aatttgaacg gtgcggagac aggcaaaatc gccgccagca
    51661 gcaacgcgcc gccagccaga ccacaagcta acatcccctg aagcgccgaa cgttttgcgc
    51721 catattgatc ggccagtcgc ccggcgtaac cacgcgtcag caccgtagcc agaaactgaa
    51781 tcccgacggc aatgccgacc atggtattgc catagcccag atcatgatga acaaacagcg
    51841 ggataaccgg caacggcaac cctacggtca tgtaggtgag aaaaaccgca aaagagatgc
    51901 ggaagagcga aaaactggca gaagatcgtg tttcggtttg gcttacagca gtcatgcatt
    51961 actccagaat gcagcgcaag gcgaggagta tccccgtctc atctctctgg tttcagggtt
    52021 acggtgcgtt ggcaggattt aacgcgtacg tcttttcaga aggaaatcga caaagcggga
    52081 agtttgccta gaactggtgg cgattgtcaa tgatgtgaaa aagggaacca tcaggttccc
    52141 ttttgcgtta gtgccggagg ccgcattggc gcgtaacgtc ggatgcgacg ctggcgcgtc
    52201 ttatccgacc tactccggcc tactgcgaat taatcttttc agcttgcgca ttaccagcgt
    52261 ggcgttggtg ccgccgaagc cgaagctgtt agacataaca gtggtcagtt cgcgatcggt
    52321 cgtttcggtc acgatgttca gacccgcagc ctgctcgtcc agctcttcaa tgttgatgct
    52381 cggggcgata aagccgtgtt ccagcatcag cagagagtag atagcttcct gcacacccgc
    52441 agcgcccagt gagtgaccgg tcatggcttt ggttgcagaa atcgccgggc tcttatcgcc
    52501 gaacacttca cggatagctg ccagctcttt cacgtcgcca accggagtcg aagtaccgtg
    52561 agagttcagg taatcgattg gggtatcaac gccatgcatc gccatctgca tacaacgtac
    52621 tgcgccttcg ccagacggag caaccatgtc tgcaccatca gaggttgcgc cgtagccaac
    52681 gatttcagca tagatgtaag caccacgcgc cagcgcgtgt tccagctctt caaccactac
    52741 cataccgccg ccgccagcga taacgaaacc gtcacggtga gcgtcgtaag tacgggaggc
    52801 tttttccggg gtgtcgttgt atttagtaga tagcgcaccc atcgcgtcga attcgcaggc
    52861 catttcccag cacagctctt cgccgccgcc agcaaacacg atgtcctgtt tgcccagttg
    52921 gatctgctct actgcgttac cgatacagtg tgcggaagtc gcacacgcgg agctgatgga
    52981 gtagttaacg ccatgaattt taaacggcgt ggcgagacag gcagaaacgc cggatgccat
    53041 cgctttagtg accacatacg ggccaaccgc tttcaggccg cgcgggccgc gcattgcgtc
    53101 agcgccgaac acctggaaac gcggggaacc gccgccggaa cctgcaatca ggccaacgcg
    53161 cgggttattc tggtaagctt ccggagagag gcccgcatca gcgattgcct gctccataga
    53221 aaggaatgca taaatggatg cgtcgctcat aaagcgcaca actttgcggt caatgaggcc
    53281 agtggtatcc agttttatgt tgccccagac gtggctacgc atgccggaat ccttcagctc
    53341 ctgagagaaa gtgatccctg aacgtccttc acgcagagat gccaggactt cctgctggtt
    53401 attaccgatg ctggaaacaa tgcccaggcc agtaatcact gcacgtttca ttcaatacct
    53461 ctgtaagtcg cacatagagt aagtttcgaa tgcacaatag cgtacacttg tacgccgaac
    53521 aagtccgatc agccatttaa tagagatatt tgcgcagcct tacacacatc gctaagatcg
    53581 agccaccgcc tgtaagacga gtaacttacg tgaaacacta ctccatacaa cctgccaacc
    53641 tcgaatttaa tgctgagggt acacctgttt cccgagattt tgacgatgtc tatttttcca
    53701 acgataacgg gctggaagag acgcgttatg tttttctggg aggtaaccaa ttagaggtac
    53761 gctttcctga gcatccacat cctctgtttg tgatagcaga gagcggcttc ggcaccggat
    53821 taaacttcct gacgctatgg aaggcatttg atcagtttcg cgaagcgcat ccgcaagcgc
    53881 aattacaacg cttacatttc attagttttg agaaatttcc cctcacccgt gcggatttag
    53941 ccttagcgca tcaacactgg ccggaactgg ctccgtgggc agaacaactt caggcgcagt
    54001 ggccaatgcc cttgcccggt tgccatcgtt tattgctcga tgaaagccgc gtgacgctgg
    54061 atttatggtt tggcgatatt aacgaactga ccagccaact ggacgattcg ctaaatcaaa
    54121 aagtagatgc ctggtttctg gacggctttg cgccagctaa aaacccggat atgtggacgc
    54181 aaaatctgtt taacgccatg gcaaggttgg cgcgtccagg cggcacgctg gcgacattta
    54241 cgtctgccgg ttttgtccgc cgcggtttgc aggaagccgg attcacgatg caaaaacgta
    54301 agggctttgg gcgcaaacgg gaaatgcttt gtggggtgat ggaacagaca ttaccactcc
    54361 cctgctccac gccgtggttt aatcgtacgg gcagcagcaa acgggaagta gcgattatcg
    54421 gcggtggtat tgccagcgcg ttgttgtcgc tggcgttatt acggcgcggc tggcaggtag
    54481 cgctttattg cgcggatgaa gcccccgcgc tgggtgcttc cggcaatcgc cagggggcgc
    54541 tgtatccgtt attaagcaaa cacgatgagg cgctaaaccg ctttttctct aatgcgttta
    54601 cttttgctcg tcggttttac gaccaattac ccgttaaatt tgatcatgac tggtgcggcg
    54661 tcacgcagtt aggctgggat gagaaaagcc agcataaaat cgcacagatg ttgtcaatgg
    54721 atttacccgc agaactggct gtagccgttg aggcaaatgc ggttgaacaa attacgggcg
    54781 ttgcgacaaa ttgcagcggc attacttatc cgcaaggtgg ttggctgtgc ccagcagaac
    54841 tgacccgtaa tgtgctggaa ctggcgcaac agcagggttt gcagatttat tatcaatatc
    54901 agttacagaa tttatcccgt aaggatgact gttggttgtt gaattttgca ggagatcagc
    54961 aagcaacaca cagcgtagtg gtactggcga acgggcatca aatcagccgt ttcagccaaa
    55021 cgtcgactct cccggtgtat tcggttgccg ggcaggtcag ccatattccg acaacgccgg
    55081 aactggcaga gctgaagcag gtgctgtgct atgacggtta tctcacgcca caaaatccgg
    55141 cgaatcaaca tcattgtatt ggtgccagtt atcatcgcgg cagcgaagat acggcgtaca
    55201 gtgaggacga tcagcagcag aatcgccagc ggttgattga ttgtttcccg caggcacagt
    55261 gggcaaaaga ggttgatgtc agtgataaag aggcgcgctg cggtgtgcgt tgtgccaccc
    55321 gcgatcatct gccaatggta ggcaatgttc ccgattatga ggcaacactc gtggaatatg
    55381 cgtcgttggc ggagcaaaaa gatgaggcgg taagcgcgcc ggtttttgac gatctcttta
    55441 tgtttgcggc tttaggttct cgcggtttgt gttctgcccc gctgtgtgcc gagattctgg
    55501 cggcgcagat gagcgacgaa ccgattccga tggatgccag tacgctggcg gcgttaaacc
    55561 cgaatcggtt atgggtgcgg aaattgttga agggtaaagc ggttaaggcg gggtaatctg
    55621 ctctcgcagt gtttgccgga tgcggcgtga acgccttatc caacctacgt gtgacgtgta
    55681 ggcctgataa gacgcgataa gcgtcgcatc aggcatggtg ctccagacgc cgcaacttac
    55741 agctgtgacg cctgctgaaa caggttttcc cacatatcgg tcaccagcgc ctggtcacgc
    55801 ggcgacaatt caccggcacc aatggctttt tccagactct ggctaacggt cgtatgcacc
    55861 gcctgagcgg agtggtcatc accactttcc agttctgcga tggctaatgt caggtggcca
    55921 cgcaaatacc cactggcaaa caactcatca tcactggcat ggtcaaccat gccgtcgatt
    55981 aatgccagaa tgcgtgattc aaactccgcg atcatcttct ttcctcattg taaaacgtgg
    56041 cgctggcttc agttgagcgc ttccggccac gggaactgtt ccgccgtaag ttccggcgtg
    56101 tgataataat tttgtagtgc tttaatgaag cgtgccgggc gttccggaat gccatttgtc
    56161 agataatcca tcacctgcgc atgtacccgc cgttgaaaca ccacacgatc cggttcgaaa
    56221 tccccttcca gattgtcgca actgacgtta aacggatatc cagccgccac gcagaataac
    56281 cagtcgaacg cctgcggctt cacttcaaca tcttcaaact ggctttgcgt ctgggcatcg
    56341 cgtccgtccg ggcaatacca gtagccgaaa tcaaccagct cacggcgcgc ttttccggca
    56401 atacaccagt gcgaaatctc atgaattgcg ctggcataaa agccatgagc aaagacgatt
    56461 cggttatacg gtacttccgc atcagcagga agatagatcg gttcgtcgtc gcctttaatc
    56521 agacgggtat taaattcatc ggcaaagcag ctattaaaaa tttcaatcaa ctgctcgtag
    56581 tggtgtgtac tgttcattag ttcatcccca accagtggag gatctcctgt ccgtggctat
    56641 cgtaaagtaa tttggcactc atcaccgccg agacgataac aatcatcggg cggatcagct
    56701 tttgtccttt gctcaacacc aaccgtgaac ccatgcgcgc gccgaggaac tgccccacca
    56761 gcatgacaaa gcccgtcgcc caaatcactt tgccgccgag aataaacagc agcagaccgc
    56821 caatgtttga cgttgcgttg agtaatttgg cgtgagccgt ggctttggcg aggttgaatc
    56881 cgcacagcat aacgaaggcc agcgcgtaaa acgatccggc agccgggcca aagaatccgt
    56941 cataaaaacc gacgcagcca ccggcaatta acgcgaaggg taaaccgtac atccggcgct
    57001 ggcgatcttc ttcacccagc tttggcatca gcaagaaata gaggccgata caaatcacca
    57061 gaatgggcaa aatctgccgt aagacatcag cctgaacgta ctgtaccaac agtgcgccgc
    57121 tcattgagcc gacaaaggtc atggcaatat tgagtttctg atcgcttaaa ctaaccactt
    57181 tgcggcgaat aaagtagata gtggcggaaa tagagccgcc gcaggcttgc agtttattgg
    57241 ttgccagcgc gttagcggga gacattcccg cagccatcaa tgccggaatg gtgagtaacc
    57301 caccgccacc ggcaatcgag tcgataaatc ccgccagcat ggcgacaaaa aagagaactc
    57361 ccagcaacag cggagaaacc ataaacaggc tattaaacgt ttccattaga tcacgtgctc
    57421 atccagtagc gcctggcagg aaggcggcaa cggaggcggt gtcttcttct caggctttgt
    57481 tgttcccggt tttggaggtt caaaccagct ttgcagttct gccccgcaac catcgcctga
    57541 tggcggtaaa ggttgatctt cacactccag actatcggca gggcaacgta atcgtacatg
    57601 catatgcgcg cgatgctgga accagggtcg cactttgcgc aaccagtcgc gatcggtgcc
    57661 cgcatcaagg caaagttgtt gtttaatcgc cggattaaca aaaatgcgcg tgacgtcttt
    57721 gtcctgggcg gcgagtttga tcaagctgaa aatttctggc ttccacagcg tggagacaac
    57781 gtgtttaccg tcgcgggaaa ctaagtccag tgcttgcggg cgcaagagct gcgcggaggt
    57841 ccagcgagtt ttcggcagtt gcagaaagat atcgacatcc agtccggtct ggtggctggc
    57901 atgaccgccg ttgaaacgcc caccagcggg catccccata tcgccaatca gcaccgtacc
    57961 catgcccaga ttgctcacct ggctactcag acgctggata aacatcacca gatccgggtg
    58021 accgaaatag cgacgctgat cggtacgcat gacctgataa tgttcggact gtatcggcaa
    58081 cgtgtcagcg ccgacaatac agccattaga aaaactgcct atcgattgtg cgctacccgg
    58141 cacaggttgg gttatttttt gccacggcgt cgctgccagg ctagcactac tggcaagcag
    58201 agccagcagc gcaatcgcgg ttttgttcat tttttaccag cgtggaatat cagtcttcac
    58261 atcggcattt tgcgcccgct gccgtaacag gtgatccatt aaaacgatcg ccagcatcgc
    58321 ttcagcgatc ggcactgcac ggatcccgac acagggatcg tgacggcctt tggtgatcat
    58381 ctcaacttct tcgccaaagc ggttaatagt gcgccccggc acggtaatgc tggaggtcgg
    58441 tttcagcgcc atatgagcaa tgatttgctg cccgctgctg ataccgccga aaatgccgcc
    58501 cgcatggttg ctctggaaac cgtctttggt gatttcgtcg cggttctggc tgccgcgcag
    58561 cgccactacg tcaaaaccgt cgccaatttc cacgcctttc accgcgttga tgctcatcag
    58621 cgcatgggcg atgtcggcat ccaggcggtc aaagaccggc tcgccaagtc cggcggggac
    58681 accactggca acaacggtga ctttcgcgcc gatggagtcg ccctcttttt tcagcgcgcg
    58741 catcagttca tctaacgcgt cgattttgtc ggggtccggg cagaagaacg gattttgttc
    58801 aaccagcgac cagtctttga tttccagcgg aatgtcgccc atctgggtca ggcagccgcg
    58861 gatttcaata ccaaatttct cggcgagata ttttttggca atcgcccctg ccgccacgcg
    58921 catggcggtt tcgcgggcgg aagaacgtcc accgccgcga taatcgcgca gaccgtattt
    58981 ttgttcgtag gtgtaatcag catggcctgg acggaagacg tctttaattg cgctgtaatc
    59041 ctgagaacgc tggtcggtat tttcgatcaa caagccaatg ctggtgccag tagtaacgcc
    59101 ttcaaaaaca ccggagagta ttttgacctg atccggctcg cggcgctggg tggtatagcg
    59161 cgatgtccca gggcgacgac gatcaaggtc atgttgcagg tccgcttccg tcagcggaat
    59221 gcctggcgga acaccatcga cgatgcagcc gagcgccagc ccgtgcgatt cgccgaaggt
    59281 ggttacgcga aagagttgtc caattgtgtt tccagccatc acggctccgt tattgttgtg
    59341 tttgcgtgtt tacttaatct ttataaatcg cgaaatgttc tcgtgcggca ataagctgct
    59401 ctttagtgag cataaacaca ccatcgccgc cgttatcaaa ctccagccag gtgaacggaa
    59461 catcgggata ttgttccata agatgtacca tgctgttgcc cacttcacaa atcaacacgc
    59521 catcatcagc aaggtaatct gccgcgttac cgagaatgcg acgcgtcagt ttcaggccgt
    59581 cggtgccaga tgccaggccc agttccggct cgtgacggta ttcgtttggc aggtcggaca
    59641 tatcttccgc gtcgacatac ggcgggttag tgacaatcag atcgtattgc actttcggca
    59701 aatcacggaa cagatcggaa cgaatcggaa tgacgttgtg gatcagaccg tgttcttcga
    59761 tgttctgttc ggcaaccgcc agcgcgtcgg gagagatatc taccgcgtcg acttccgctt
    59821 ccgggaaggc ataagcacag gcaatggcga tgcagccgct accggtacac atatctaaaa
    59881 tatgctgcgg ttgcttgctg atgagtccgg caaatttatt gttgatcagt tcaccaatcg
    59941 gcgagcgcgg caccagcacg cgttcatcga cgtaaaattc atgaccgcag aaccaggctt
    60001 tgttggtcag gtaagccacc ggaatgcgct cgttgacgcg gcggatcacg cgttcaacaa
    60061 tacggtgttt ttcgctggag gtcagacgcg cggtgcgcat atcttccggg atatccagcg
    60121 gcaggtagag cgatggtaac accagctgta cggcttcatc ccacgggtta tcggtgccgt
    60181 gaccgtacca gatatttgcc gcgctgaagc ggcttaccga ccagcgcaat atgtcctgaa
    60241 tggtttgcag ctcatttact gcttcatcaa cgaaaatttt atccacgtat tcctccaggg
    60301 catgatcgca ataatttcgg cggctagttt gccatgaaga tgacgataaa tcagcattca
    60361 cgcgcggtga gtgaggaaaa atacgtttaa aacgatcgat tgtgctacga gtcgggtaaa
    60421 ctgtaggaaa attagaaata gagacagata aatgaaaaag aaaacaacac ttagcgagga
    60481 ggaccaggct ctgtttcgcc agttgatggc ggggactcgc aagattaagc aggacacgat
    60541 tgtccaccga ccgcagcgta aaaaaatcag cgaagtgccg gtgaaacgct tgatccagga
    60601 gcaggctgat gccagccatt atttctccga tgagtttcag ccgttattaa ataccgaagg
    60661 tccggtgaaa tatgttcgcc cggatgtcag ccattttgag gcgaagaaac tgcgccgcgg
    60721 cgattattca ccggaattgt ttttggattt acacggtctg acgcagctgc aggccaagca
    60781 ggaactgggc gcgttgattg ccgcctgtcg ccgcgaacat gtgttttgtg cctgcgtgat
    60841 gcatgggcac gggaagcata ttttgaagca acaaacaccg ctgtggctgg cacaacatcc
    60901 gcatgtgatg gcatttcacc aggcaccgaa agagtacggc ggtgatgcgg ctttgttggt
    60961 gttgattgaa gtggaagagt ggttgccgcc ggagttgccc tgaatgctgg cagcaaatgc
    61021 cggagcagcg taaacgcctt atccggccta caaatatgtg ataattcaat aaattgcaat
    61081 tcacttcgta ggcctgataa gacgcatcag agcaatacta cgaacttcag ttcattcaat
    61141 acatactgaa aacgacacgt cgaatcaatc ggttgcccac tactggtatt cacattcacc
    61201 acaatatcgt gtagtacttt atccgttacc ggatgacgag ttggcgtaac cgttaccgtt
    61261 gatttatcac aaccgtaaga ggtaaaggtt aacaattctg ataccgcgcg gcagtatgct
    61321 cctctgccat cattatcaga aaaattactg ccatcacaga gtctgccgcc ttccatcagg
    61381 taggtcgaaa gcgtggtggt cactgttgac cccgtggact gaattcgtaa cgccttatta
    61441 tggctgacat aattttcact ccaggagata tcgtatgaag cgaccggtat cgttgggcat
    61501 attgtgttat gcactgtagg ggccgtatag cttccaaatc cgggtaaatc tgtataggaa
    61561 taaaaggatg cgatccaagg gcaaccataa tcatcagatg caacggtaat tttaaacgtc
    61621 atatatgcac aacgaaaaat tttcgtatct atgccagggc aatttgagtt attaattgta
    61681 tcaaagtcag tcagtaccaa agttggagct gaacccgacc cagaccagga aataacccgc
    61741 aacgtatcgc ctttatacgc aaaagaatca tatttcccat atgcaacctc ctttggcgtt
    61801 atcaccgtaa cattgaaaac actgctcgct acagcaccgg ttaccttgtc catggcataa
    61861 taaactctca cactgtcaaa actcgcactc ataagtttat ctccagcata actcttagat
    61921 gcgccgacaa cactcagaac gagcagcatt aggctcgcga aaattatggt ccattttttc
    61981 atttcagaaa tactcaaagg ttgccgtcac tgtggcacta aagtctccgg acgttacatc
    62041 tcgcccgctc tttgcctgta accaggtatt aaaatttagg ctgttatttc cctgattcag
    62101 cgggagcgtg acgcctgttg tattgttgat tggcaccgat gtgcctgccg cagtttcaat
    62161 accaatccca gcgccggatg ctgatgacga ggagtcaaag gccagaaagc cgggtagagc
    62221 actatcttct gttcctgtca acgttacttt gacattaaac aatgtagaac tatgacaatc
    62281 tttcaattga aacaccaatg gcactcgctc tgactgccca aggttcatta aatcactggc
    62341 ggcaatggca ggaaaatgta cttctgccaa ctggctacca ttaatcactg gcgtacacga
    62401 tttactgatt aaattgccgg taaagtgcaa attatctcct gctattgtgg ttccactgcc
    62461 cagcaacatc aggctactaa taaaaaacaa cgtacgcttt ttcttcattg gtaatctacc
    62521 tgtaacgtgg cccaggcctc aaagtccgcc tctggcagaa tgacaccact cttttttact
    62581 ggcacagccg ttaacaccgg taaatcggtt tcattcacca caagtggcgt attgatcgta
    62641 aaactctgcc ctgcctgttt gagctgaatg cctaaaccag tgatattgct gcttactgca
    62701 gcattatcaa aatcgcttgg tgtgccactc cagctcaagg tcatttccat taatgggtcg
    62761 cgaaccgccg tatcgcaggt aatggaatac ggcacggttt gctcataatt gctgccatta
    62821 atgtcatcaa taagcacatc ctgaaaagtt atttcaatgg tttgagcgtt gctaatcgtg
    62881 cagttaggcg gctgcactaa ataaccgtga aaagagacgt ttgagagtgc catcgagcaa
    62941 aagccccaca gcaaaataag cgagatccgt ttcattggta atccaccaca attgtggcac
    63001 tggcgttaaa ttcagctgcc gctaacgttg taccactctg tttcaccggc acagcttcca
    63061 gaacgggaac tcccgaggaa aaattaaatg gcaaccatga gccagaggtt aagtcgagta
    63121 ttgtgtgatc actacttttc tgaacccgaa taccaaaccc aggaataccc gtgctgataa
    63181 ctgtttcacc gttaatcacg accgttgtcg cctgcatctg tagacgcagg tcatccattg
    63241 ccagattgtt acaaacaagc gagtagtcaa tgggacgttt ataatcaacc ctattgatct
    63301 tattaatgaa tacattacca aactcaactg ccgcaccatt taccgtacaa ggaggagggg
    63361 catcgacaag cacccgtaaa ttcatactta ctgacttact cgctgcaact gcaaagccgc
    63421 taccaacaca acatatcagt aacccagtta ccgcccagac agaacaagat cctttcatac
    63481 tggctctccc ggcctcagcc tttgcggctc tgctcttcat cgacctagca actgttaccg
    63541 ttgcaagtga aaaagagcgg catccgagcg ccatagtcgt tgacataggt cagtaccggc
    63601 acgctaccca tcttcacatt cagcggtgca gaggttttcg gcgacatcac tattggcgag
    63661 aaaccggctg ccggattgcc atttttctgt gtgctggcat tactgaaaat gacgtaatac
    63721 gacgtcgggt tattgacggt aaattcctga ccgttgcggg ttaaagtaat cttatattgc
    63781 taggggtttt tcatgctgac gttctccagc gccttcggtc gccagaacaa cttaatacgc
    63841 gtttgtagtg ctatttgcag cgtgttggct ttgtttgatt ttggcgggat ctcacgcacg
    63901 ttgaagtaga acaggctttc acgatcggca ggcaatttgt tgatatccgg cattccctgc
    63961 acttttacct gcccgttcat cattgaatcg atgcgttgca ccggtggcag aacagttaac
    64021 ggtgaactga tcttgttacc cttttcatct tcgatccagc tttgggccag ataaggcagt
    64081 ttcggatcgt tattgcgcaa cgtcacgctg attgatttgt cgctttcatt aaatatcaaa
    64141 cgggtacgat ctggcgttac agaagcctgg gcagacaaac tggcagcgct aagcagtaac
    64201 gctagcgtcg tagcacccaa tttggcagag caaagtaaat cagacatgga tcattcctta
    64261 atcacgttat tggttagctg aaacagaaac agggggattg gtgggtgtca cctgttgcgt
    64321 ctgctcctga atgacaggct ttatgtcatc aggaacaacc ggcgcgacgt tgcctttatg
    64381 ctggcatggc agtaacaagc cgttaaacag atcggcaggc agcgggtccg gcaggttgat
    64441 atcgcaatgc gcaacaccac tccagaacac actcatgtgt tcgccaggtt tcactccagc
    64501 cagataaaca ttgccgtcat catcgacaag gcccactgtc tgctcgttat cattttttac
    64561 ttctgcgcca aacggaggat ggctgccgtc ttgtaaacgc agcaccgcca tcgctttttg
    64621 accactgatg acggcaaatt tgcggtagcc aatcgcccct tcagttagcg tggcttgtac
    64681 caccgactgg gttgcttcgg cgttttccgg cagtttgttg aggtcgatat acgcctgatt
    64741 gcggtaatag ttattgacgt cagaaacgac ggctttacca aacatattgg tataaacagc
    64801 agcaccgtta ccttctaccg gaacatcggc aacgccatcg gcatcaatca acaagcgtgt
    64861 cccgcccata ttctgggtac ggtgaagtgc gccaccgtgg gcagtaagcg ttgcgccgcc
    64921 ctgtaacgac aagcccgcag aggtgtattg cccttcatgg tagttcgcac tgaggtcaac
    64981 ctgcgccagc gaaccatcat ggctgtaata gccgtcaacg ctggtgtgtt tgtcactggt
    65041 gccgacgttc aactgatagt gagtcgcgtc atcgacacgg ctgaaataac cgacctgact
    65101 gctgtccgtc ccactgccat agtaccgtta tagctaacgg tactgttgtc gccccacggc
    65161 atactgagcg aaatgtacat gcctttgtcg gcctggttgt catactcgta acggtagcca
    65221 gtcatcgaga tgctgacatt acgaatactg cccatattga aatagtgcga gagcatgatg
    65281 ttgtagtttg tctgctcctc gcgatcccag taggtatggc gggtatagtt gagataaacc
    65341 gaaacacccg catcgcggaa gttctggtta taagtggcgg tgtacatctc tttgtcgttg
    65401 cccgtgcgta ccattccgct gtcactggca tccagatact cgctcatggt cataaagttc
    65461 tcttccgaga agcgatatcc agcaaaagta acgcggctgt tgagctggtc aaagtctttg
    65521 gaataactca cacggaagga gttaccgtcc agcgaacctt tgccataagc ggtgtcttta
    65581 tccagtttgg tgtgcgagtg agtaacatca aacgcgaccg cgccgaatgt agacaaatcg
    65641 cgaccgacac caagcgccgc agactgatag ttttcatctc ccagtgcgcc gccatataac
    65701 gaccagccgt tagcgatccc ccaggaagct tcagcaccag aaaaaaattc accctcgaca
    65761 tggtgtcccc actcttgcgg acggcccatc atgatcttat agcgaacctg acctggacga
    65821 gtgaggtatg gcatcgaggc ggtgctgatg tcatattcct gcacctggcc gttctgttct
    65881 tcaatgcgaa tatgcaacgt accggagaca gaatcaccaa gatcctgaat acgaaacggt
    65941 ccagccggaa cctgcgtttc gtaaatcaca cgccccatct ggctgacggt cacttttgct
    66001 gtggtgtgtg ccacgccgga aatgtctggc gcgtagccgc gcagattggg aggcaacatt
    66061 tgatcgtcag tactgacact gccaccaaca tagttaaaac catcaaaaat atcggatctg
    66121 aggtaatcct cgcccagcgc cagtttggct tttaatgatg gtaacgcccg cctggcatag
    66181 tagcgactcc actcccattt ttttgagttt catcgccgct gaattcatcg tcatcattac
    66241 tgcgagtatg ttgatagtta gtctgccagt cagcacgcat acgccacggc cctaggttaa
    66301 ccccaaccgt cccgttgccg ctgatctcgt tactatcatc accgccattt tcttcgtgcc
    66361 gggtttgtgc gttgatgctg tagtccgcga cgatcccgga gatgccgtca tcccaacgtg
    66421 aaggcggatc cctgtcggga taagtatatt cgaggtaagc ctgtggcagt gaaatgacta
    66481 atgcggactg gcttaaatca gccttaattt ccatgccttc cagttgaccg gatttcaggc
    66541 atttagcatc gtggctccat tgcagatttt tcgccacgtc ttcttttaaa ccaaactgcg
    66601 ctaccagttc cggtgtcaga caagcatagc ttttgctcgc gtcatcttca ccagcatacc
    66661 agtaaatatc gtactcttcc gccaatggct gtttatttag ttgaacctgt aaattgtatt
    66721 ttccgggctc aacatatccc tggctggaaa aacgcttcat atcaattttt gtgtcgcctt
    66781 ttaattccag aaaacgggaa tcaaactgaa tgtcgtcttc agcccagaca ctgctataac
    66841 tccctgacat tgccagcgca atgcaccacg gaagaatccg taatcgaaaa agagaatggt
    66901 caggcataca cgtttctatc cgtttttatt cttgttcggt caaaatacat ttaaatcccc
    66961 catagcagtg tcgctatggg ggtatgcctt acaggtaagt aatctggaag gtggtatttg
    67021 cttcaaaatt acccagatct ggcgcatcag ctgcgccgac cagccaggcg ttaaagttca
    67081 gtgtctgctt cgctttgcct ttgttggtag aagtgtcttt cactattttc tggtcaatac
    67141 ccatgccgct tttgtaagag gtacccagag agtcaccgat cgccaggcta acattgttaa
    67201 atgccgcacc ggtatcggta ttgaaaatgg tgtaataatt gccgctattt gcagaagaaa
    67261 cggtaccagt aaaggtagtg gtcattgttt cctgagtatc aaatacgcaa tcttgcagac
    67321 gaatctggaa acttttcggc gtggtggtgc cgttattttt cagtttttcg gcaccgatat
    67381 cacccatatc aacttccagt ttgtgatcgt ccggaacgat agaacacact gagtcttcaa
    67441 tggtgccgta aaaacgtgca gtaccgttgt tacccgcagc gattgttgcc gtagaggtga
    67501 acacgcccat caccattgcc gcagcaatag ctgttttaac aaacttactc attatttgtc
    67561 cttaaactat aaataattaa aaatataaac attgcaatac attgattcag tcaatagcca
    67621 atgtttatac ccagccattg aatacggcat gcgacccaaa aagaacaggt cgtagaaaat
    67681 tgtattcatt ggtgtggggc cgcatagtga tgtttccaca cccctgatcc aatttaattt
    67741 tactaaagag taaatgtaga tttaattaat atattgacag aagaaaggtt atcttaagga
//
LOCUS       DAETQL010000008        66987 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.008, whole genome shotgun sequence.
ACCESSION   DAETQL010000008 DAETQL010000000
VERSION     DAETQL010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.008"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="K0W65_000561"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="K0W65_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBI3093955.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="K0W65_000562"
     CDS             complement(1151..1237)
                     /locus_tag="K0W65_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBI3093956.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="K0W65_000563"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="K0W65_000563"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBI3093957.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVYVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="K0W65_000564"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="K0W65_000564"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBI3093958.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3586..3630
                     /locus_tag="K0W65_000565"
     CDS             3586..3630
                     /locus_tag="K0W65_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3093959.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="K0W65_000566"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="K0W65_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBI3093960.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="K0W65_000567"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="K0W65_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBI3093961.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="K0W65_000568"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="K0W65_000568"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBI3093962.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="K0W65_000569"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="K0W65_000569"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBI3093963.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="K0W65_000570"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="K0W65_000570"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBI3093964.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="K0W65_000571"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="K0W65_000571"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBI3093965.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="K0W65_000572"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="K0W65_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HBI3093966.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="K0W65_000573"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="K0W65_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBI3093967.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="K0W65_000574"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="K0W65_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HBI3093968.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="K0W65_000575"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="K0W65_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBI3093969.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="K0W65_000576"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="K0W65_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBI3093970.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="K0W65_000577"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="K0W65_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBI3093971.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="K0W65_000578"
     CDS             complement(16981..17745)
                     /locus_tag="K0W65_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI3093972.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="K0W65_000579"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="K0W65_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBI3093973.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="K0W65_000580"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="K0W65_000580"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBI3093974.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="K0W65_000581"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="K0W65_000581"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBI3093975.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="K0W65_000582"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="K0W65_000582"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBI3093976.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="K0W65_000583"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="K0W65_000583"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBI3093977.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="K0W65_000584"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="K0W65_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBI3093978.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="K0W65_000585"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="K0W65_000585"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBI3093979.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="K0W65_000586"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="K0W65_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBI3093980.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="K0W65_000587"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="K0W65_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBI3093981.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="K0W65_000588"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="K0W65_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBI3093982.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="K0W65_000589"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="K0W65_000589"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBI3093983.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="K0W65_000590"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="K0W65_000590"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBI3093984.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="K0W65_000591"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="K0W65_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBI3093985.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="K0W65_000592"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="K0W65_000592"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HBI3093986.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="K0W65_000593"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="K0W65_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBI3093987.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="K0W65_000594"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="K0W65_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBI3093988.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREA
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="K0W65_000595"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="K0W65_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBI3093989.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="K0W65_000596"
     CDS             complement(40421..41815)
                     /locus_tag="K0W65_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3093990.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="K0W65_000597"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="K0W65_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBI3093991.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="K0W65_000598"
     CDS             complement(42158..43444)
                     /locus_tag="K0W65_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI3093992.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="K0W65_000599"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="K0W65_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="K0W65_000600"
     CDS             complement(44451..45221)
                     /locus_tag="K0W65_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBI3093993.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45577..47091
                     /gene="caiT"
                     /locus_tag="K0W65_000601"
     CDS             45577..47091
                     /gene="caiT"
                     /locus_tag="K0W65_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HBI3093994.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47122..48264
                     /gene="caiA"
                     /locus_tag="K0W65_000602"
     CDS             47122..48264
                     /gene="caiA"
                     /locus_tag="K0W65_000602"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBI3093995.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48393..49610
                     /gene="caiB"
                     /locus_tag="K0W65_000603"
     CDS             48393..49610
                     /gene="caiB"
                     /locus_tag="K0W65_000603"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HBI3093996.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49684..51237
                     /gene="caiC"
                     /locus_tag="K0W65_000604"
     CDS             49684..51237
                     /gene="caiC"
                     /locus_tag="K0W65_000604"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBI3093997.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51346..52131
                     /gene="caiD"
                     /locus_tag="K0W65_000605"
     CDS             51346..52131
                     /gene="caiD"
                     /locus_tag="K0W65_000605"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBI3093998.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52137..52727
                     /gene="caiE"
                     /locus_tag="K0W65_000606"
     CDS             52137..52727
                     /gene="caiE"
                     /locus_tag="K0W65_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBI3093999.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="K0W65_000607"
     CDS             complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="K0W65_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBI3094000.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMCR"
     gene            complement(53470..56691)
                     /gene="carB"
                     /locus_tag="K0W65_000608"
     CDS             complement(53470..56691)
                     /gene="carB"
                     /locus_tag="K0W65_000608"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBI3094001.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56709..57857)
                     /gene="carA"
                     /locus_tag="K0W65_000609"
     CDS             complement(56709..57857)
                     /gene="carA"
                     /locus_tag="K0W65_000609"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBI3094002.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="K0W65_000610"
     CDS             complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="K0W65_000610"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBI3094003.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="K0W65_000611"
     CDS             complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="K0W65_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBI3094004.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60281..61231)
                     /gene="ispH"
                     /locus_tag="K0W65_000612"
                     /gene_synonym="lytB"
     CDS             complement(60281..61231)
                     /gene="ispH"
                     /locus_tag="K0W65_000612"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBI3094005.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61233..61682)
                     /gene="fkpB"
                     /locus_tag="K0W65_000613"
     CDS             complement(61233..61682)
                     /gene="fkpB"
                     /locus_tag="K0W65_000613"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBI3094006.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61769..62263)
                     /gene="lspA"
                     /locus_tag="K0W65_000614"
     CDS             complement(61769..62263)
                     /gene="lspA"
                     /locus_tag="K0W65_000614"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBI3094007.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62263..65079)
                     /gene="ileS"
                     /locus_tag="K0W65_000615"
     CDS             complement(62263..65079)
                     /gene="ileS"
                     /locus_tag="K0W65_000615"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBI3094008.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65122..66063)
                     /gene="ribF"
                     /locus_tag="K0W65_000616"
     CDS             complement(65122..66063)
                     /gene="ribF"
                     /locus_tag="K0W65_000616"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBI3094009.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66071..66289)
                     /locus_tag="K0W65_000617"
     CDS             complement(66071..66289)
                     /locus_tag="K0W65_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HBI3094010.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66392..66655
                     /gene="rpsT"
                     /locus_tag="K0W65_000618"
     CDS             66392..66655
                     /gene="rpsT"
                     /locus_tag="K0W65_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBI3094011.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16249 a        18239 c        17246 g        15253 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggattagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatagacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccattc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aggcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccctgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaacgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 gcccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgttcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caacgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggt ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cgctctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc tcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacaga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccaactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt catcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaggacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggctatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctggtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgataaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agtgcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagtcacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggcac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggtggaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cgctatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgctga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca agtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatatt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtgc aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacgct tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccaac gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc accatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaagtc agatgctatt tttttaccca caaaagaata
    45361 taacaaactg attaataacc aaaattaacg aaaaacgccc tctgttcact cgaaacaaaa
    45421 atgtgatacc aatcacagaa tacagcttat tgaataccca ttatgagtta gccattaacg
    45481 cgtccacgag gttaataata attatattaa atgttaacaa aaataaaaca aacgggaacg
    45541 caaaataaat attcgttttc acagtggaat taactcatga agaatgaaaa gagaaaaacg
    45601 ggaatagaac cgaaggtttt ctttccgcca ttaataatcg tcggcatact ttgttggctt
    45661 acagtcagag atctggatgc ggcgaatgtc gttattaatg ctgtattcag ttacgtcacc
    45721 aatgtatggg gatgggcatt tgaatggtat atggtggtga tgcttttcgg ttggttctgg
    45781 ctggtgtttg gcccgtatgc caaaaagcgt ttaggtaacg aaccgccaga atttagcacc
    45841 gccagttgga tctttatgat gttcgcctcc tgtacgtctg ctgccgtact gttctgggga
    45901 tcgattgaga tctactacta catctccacc ccgccgtttg gcttagaacc gaactcgaca
    45961 ggagcgaaag agttggggct ggcttacagc ttgttccact ggggacctct gccgtgggcc
    46021 acttacagct tcctttcagt cgccttcgct tacttcttct ttgtccgcaa aatggaagtg
    46081 attcgcccca gctcgacact ggtgccgctg gcaggtgaaa aacacgccaa agggttgttc
    46141 ggtaccatcg tcgacaactt ctatctcgtc gccttgatct tcgcgatggg taccagtctg
    46201 ggccttgcca cgccgctggt gaccgagtgt atgcaatggt tgtttggcat tccgcatacc
    46261 ctgcaactgg acgctatcat cattacctgc tggattatcc tcaacgccat ttgcgtcgct
    46321 tgcggtctgc aaaaaggggt acgtatcgcc agtgacgtgc gtagttacct gagcttcctg
    46381 atgctgggtt gggtgttcat cgtcagcggt gccagcttca tcatgaacta cttcaccgat
    46441 tcggtgggga tgttgctgat gtatctgccg cgcatgttgt tctataccga tcccatcgct
    46501 aaaggcggct tcccgcaggg ctggaccgtg ttctactggg catggtgggt gatttacgcc
    46561 atccagatga gtatcttcct cgcccgcatc tcccgtggtc gtactgtgcg tgaactgtgc
    46621 ttcggcatgg tgatggggct gacagcatcc acctggatcc tgtggaccgt gctcggtagt
    46681 aacactctgc tgttgatgga taaaaacatc atcaacattc caaatctgat cgaacagtac
    46741 ggtgtggcgc gcgccatcat cgaaacctgg gccgctctgc cactcagcac cgccaccatg
    46801 tggggcttct tcatcctctg ctttattgcc accgttacgc tggttaacgc ctgctcttat
    46861 accctggcga tgtctacttg ccgcgaagta cgcgatggtg aagaaccacc gctgctggtg
    46921 cgtatcggtt ggtcaattct ggttggcatt atcggtattg ttctgctggc gctcggcggc
    46981 ctgaaaccga ttcaaaccgc cattatcgcc ggaggatgcc cgctgttctt cgtcaacatt
    47041 atggtgacgc tctcctttat taaagacgcg aaacagaact ggaaagatta attaaccccc
    47101 aaaatatcaa gaggttgaaa gatggatttt aatttaaatg atgagcagga actgtttgtc
    47161 gccggtatcc gcgaactgat ggccagcgaa aactgggagg cctattttgc cgagtgcgac
    47221 cgtgacagcg tctacccgga acgttttgtc aaagcactgg cggatatggg tatcgacagt
    47281 ctgctgatcc ctgaagagca cggtggtctg gacgcggggt ttgttactct cgccgccgtg
    47341 tggatggagc tgggacgtct gggggcacca acctatgtac tgtaccagct gccgggcggg
    47401 ttcaatacct tcctgcgcga aggcacacaa gagcagatcg acaagattat ggctttccgc
    47461 ggcaccggta agcagatgtg gaactcagcg attaccgaac cgggcgcggg ctccgacgtg
    47521 ggtagcctga aaacgactta tacccgtaga aatggtaaga tttatcttaa tggtagtaag
    47581 tgttttatta ccagtagcgc ctacaccccg tacatcgtgg tgatggcgcg cgacggggct
    47641 tctccggaca aacctgtcta caccgaatgg tttgttgata tgagcaaacc gggcatcaaa
    47701 gtgaccaaac ttgagaagct cggtctgcgt atggatagct gctgtgaaat cacctttgac
    47761 gatgtggaac tggacgagaa agacatgttc ggtcgggaag gtaacggctt taaccgcgtc
    47821 aaagaagagt tcgaccatga acgtttcctg gtagccctga ccaactacgg tacggcgatg
    47881 tgcgcctttg aagatgcggc gcgctacgcc aaccagcgcg tgcagtttgg cgaggctatt
    47941 ggtcgtttcc agttgattca ggaaaaattc gcccacatgg cgatcaaatt aaactccatg
    48001 aaaaacatgc tgtatgaagc agcgtggaaa gcagacaacg gcaccatcac ctctggcgat
    48061 gcagcgatgt gcaaatactt ctgcgccaat gcggcatttg aagtggtgga tagcgcaatg
    48121 caggtgctgg gcggtgtcgg gattgcgggc aaccaccgca tcagccgctt ctggcgtgac
    48181 ctgcgtgtag accgcgtttc cggaggatct gacgaaatgc agatcctgac gctgggtcgt
    48241 gcggtgctga agcaataccg ctaagtccgt gcgctgcctg atgcgacgct gacgcgtctt
    48301 atcaggccta cgggagcacg aatgtaagcc ggataaggcg tttacgccgc atccggcagt
    48361 catgcgctac ataaattttt caggagctaa taatggatca tctacccatg ccgaaattcg
    48421 gaccgctggc cggattgcgc gttgtcttct ccggtatcga aatcgccgga ccgtttgccg
    48481 gacaaatgtt cgcagaatgg ggcgcggaag ttatttggat cgagaacgtc gcctgggccg
    48541 acaccattcg cgttcaaccg aactacccgc agctctcccg ccgcaatttg cacgcgctgt
    48601 cgttaaatat tttcaaagat gaaggccgcg aagcgtttct gaaattaatg gaaaccaccg
    48661 atatcttcat cgaagccagt aaaggtccgg cctttgcccg tcgtggcatt accgatgaag
    48721 tactgtggca gcacaacccg aaactggtta tcgctcacct gtccggtttt ggtcagtacg
    48781 gcaccgagga gtacaccaat cttccggcct ataacaccat cgcccaggcc ttcagtggtt
    48841 acctgattca gaacggtgat gttgaccagc caatgcctgc cttcccgtat accgccgatt
    48901 acttttctgg cctgaccgcc accacggcgg cgctggcagc actgcataaa gtgcgtgaaa
    48961 ccggtaaagg cgaaagtatc gacatcgcca tgtatgaagt gatgctgcgt atgggccagt
    49021 acttcatgat ggattacttc aacggcggcg aaatgtgccc gcgcatgagc aaaggtaaag
    49081 atccctacta cgccggttgc ggtctgtata aatgtgccga cggctacatc gtgatggaac
    49141 tggtgggcat tacccaaatt gaagagtgct ttaaagatat tggcctcgca catctactcg
    49201 gtacgccgga aatcccggaa ggcactcagc ttatccaccg tatcgaatgc ccttacggcc
    49261 cactggtaga agaaaaactc gatgcctggc tggcggcaca taccatcgca gaagttaaag
    49321 aacgctttgc cgaactgaat atcgcctgtg ccaaagtact gaccgtaccg gaactggaaa
    49381 gcaatccaca gtatgttgcc cgtgaatcaa tcactcagtg gcaaacgatg gatggtcgca
    49441 cctgcaaagg gccgaacatc atgccgaaat tcaaaaataa ccccggacaa atctggcgcg
    49501 gaatgccctc acatggcatg gacacggctg ccattttgaa aaatatcggc tacagcgaaa
    49561 acgacattca ggagttggtc agcaaaggtc tggccaaagt tgaggactaa acgcgtggcg
    49621 tcagtcggct tgcctgacta gcgcccgccc aataacaaac gaacaaaaat ggatagaggt
    49681 gcaatggata tcattggcgg acaacatcta cgtcaaatgt gggacgatct ggcggacgtt
    49741 tacggtcata aaacggcgct gatttgtgaa tccagcggcg gagtcgttaa ccggtatagt
    49801 tatcttgagt taaatcagga gattaaccgc acggcaaacc tgttttatac gctggggatt
    49861 cgcaaaggca acaaggttgc actacatctc gacaactgcc cggaatttat cttttgctgg
    49921 ttcgggctgg caaaaattgg cgcgattatg gtgccgatta acgcccgcct gttacgcgag
    49981 gaaagcgagt ggatcttgca aaatagccag gcgtgcctgc tggtgaccag tgcgcaattt
    50041 tatcctatgt atcaacagat tcagcaggaa gatgccactc aattgcggca catttgcctg
    50101 acagatgtgg cacttcccgc tgatgatgga gtgagttcat ttactcaact gaaaaatcaa
    50161 caacctgcca ccttgtgcta tgcaccgccg ctatcgactg acgatacggc ggaaattctc
    50221 ttcacctccg gcaccacctc ccgaccgaaa ggtgtggtga ttacccatta caacctgcgc
    50281 ttcgctggat attactccgc ctggcagtgt gcactgcgtg acgatgacgt ctacatgacg
    50341 gtaatgcctg cgtttcatat cgattgccag tgtactgcgg cgatggcggc gttttctgcc
    50401 ggggccacct ttgtgctggt cgagaaatac agcgcccgcg ccttctgggg acaggcgcag
    50461 aagtaccgcg ccaccattac cgaatgtatt ccgatgatga tccgtacgtt gatggtgcag
    50521 ccgccttcag cgaacgatcg gcaacaccgc ctgcgggaag tgatgtttta tctcaacttg
    50581 tcggagcagg aaaaagacgc attttgtgaa cgcttcggcg ttcgcttgct gacgtcttat
    50641 gggatgacgg aaaccattgt gggcattatc ggcgatcgcc ctagcgataa acgacgctgg
    50701 ccgtcgattg gtcgggcggg gttttgctac gaagcggaga tccgcgacga tcacaatcgc
    50761 ccgctcccgg caggtgagat cggtgaaatc tgtattaaag gcgtaccagg gaaaaccatc
    50821 ttcaaagagt attttctcaa cccgaaagcc actgcgaaag tgctggaagc cgacggctgg
    50881 ctacataccg gcgataccgg ataccgcgac gaagaaggct ttttttattt cgtcgatcgc
    50941 cgctgcaata tgatcaaacg cggtggcgag aatgtctcct gcgtggagct ggaaaatatc
    51001 atcgccaccc atccgaaaat tcaggacatc gtggttgtgg gtattaaaga ttcgattcgc
    51061 gatgaagcca tcaaagcatt tgtggtgctg aatgaaggtg aaacattgag cgaagaggaa
    51121 ttttttcgct tctgcgaaca aaatatggcg aaatttaaag tgccctctta tctggagatc
    51181 agaaaagatc tgccacgtaa ttgctcgggg aaaataatta gaaagaatct gaaataaatg
    51241 aaacgtcagg gaactaccct gccagctaat aatcacgccc taaaacaata tgcatttttt
    51301 gcagggatgt tgtcgtccct aaaaaagcaa aaatggagaa aaggaatgag tgaatcatta
    51361 catctgaccc gcaatggatc aattctggaa attacccttg atcgcccaaa agcgaatgct
    51421 attgatgcaa aaaccagctt tgaaatgggc gaagtatttc taaatttccg tgacgatccg
    51481 caattacgtg tcgccattat taccggtgcc ggagagaaat tcttttccgc gggctgggat
    51541 ttaaaagcgg cagcagaagg cgaagcaccg gatgctgact ttggcccggg tggttttgcg
    51601 ggattaaccg aaattttcaa tctcgacaaa ccagttatcg cagctgtgaa cggctacgcc
    51661 tttggcggcg gtttcgaact ggcgctggcg gcagatttta ttgtttgtgc cgataacgcc
    51721 agcttcgccc tgccggaagc caaactgggc atcgttcctg acagcggcgg tgtgctgcgt
    51781 ctgccgaaga tcctgccgcc taccatcgtc aatgaaatgg tgatgaccgg cagacgaatg
    51841 ggcgcagaag aggcgctgcg ttgggggata gtcaaccgcg tggttagcca ggcggaactg
    51901 atggataacg cccgcgaact ggctcagcag ctggttaaca gcgccccgct ggcgattgcg
    51961 gcgctgaaag agatctaccg caccaccagc gaaatgccgg tagaagaagc gtatcgctat
    52021 attcgcagcg gcgtgttgaa acactatcca tcggttctgc attcggaaga tgccattgaa
    52081 gggccgctgg cgtttgccga gaagcgcgat ccggtgtgga aaggacgtta acaaccatga
    52141 gctattacgc ctttgagggg ttaattccgg tggttcaccc gacggcgttt gtccatccca
    52201 gtgccgtctt gattggcgat gtgattgtgg gagccggtgt ctacatcggc ccactcgcct
    52261 cactgcgtgg tgactacggg cggttgatcg tgcaagcggg agccaatatt caggatggct
    52321 gcattatgca tggctactgc gacactgaca ccatcgttgg ggaaaacggc catatcgggc
    52381 atggagcgat cctgcatggt tgtgtgattg gtcgcgatgc attggtcggg atgaacagcg
    52441 taattatgga tggcgcggtc attggcgaag agagcattgt tgccgcaatg agctttgtca
    52501 aagcaggctt tagtggcgag aaacgccagt tgttgatggg tacgccagcc cgcgctgtac
    52561 gcagtgttag tgacgacgag ttacactgga aacggttgaa taccaaagag tatcaggatc
    52621 ttgtcgggcg ctgtcatgca tcgttacatg aaacgcagcc actgaggcaa atggaggaaa
    52681 atcgcccccg tttgcagggg acgacggatg tgacgcctaa acggtgatct tgccggatgc
    52741 gacctggttg ctggataaga tgctacaaca tcgtatctgg caagcaggac gcccggtatc
    52801 aacgagataa aattaacgac acatactctt tgacaacatc atctgccact tctgctcgcg
    52861 gttaagttcc gtgagcggaa cggcaggaat acgcggtgtc tttcttacgc cgactaattt
    52921 ttcccgactg ttattgcgca ggcgcgcgta aatttgctca tcgatatcga ccactttaac
    52981 cagtcgctga cactggcatc cccgcccttc cagcttatta gggatcatct taacttcgca
    53041 gcttatttct gtgacttccg acagaatata agtcagggta ttaaccgcct tgctgtgttc
    53101 aatatcgaaa tgaatagaga tctctcttgc tatcacccac cgattttcag ccatcatcca
    53161 ttcggcgatt aacaagtaga gtggtttttc aacatatcct tcacacatac gaactccagt
    53221 ttcacaaatt aaaagtctag ctatgactcg ttttgaagca attaaacaag gccatattaa
    53281 aattgtggat atatcgatag tatgcaactt caccgtcgat aagtgtgagc taaatcctgc
    53341 ttatgtcatc aaaaatatcg attccccaaa agatttactt aatgggcaaa aaaaaacggt
    53401 cctcatcaga gaaccgtatt agttattcga tcaaattagc ggatgaaaaa tatccgccac
    53461 gacacgctat tatttgatct gtgcgtgcat ttcctgcacc gaaattactt tttcagtcgc
    53521 atcggcattc agcgccatcg cggtagcgaa accaccgttc agggtggtgt cataatgcac
    53581 tttatattgc agcgcactgc gacggatcac gcgggagtct tcaatcgcac gacggcctga
    53641 ggtggtgttg atgatgtagg tatattcgcc attcttgata cggtcctgaa tgtgcggacg
    53701 gccttcatgc accttgttta ccagacgcgg attgataccc gcttcgccca gcacaatcgc
    53761 cgtgccgtgg gtcgcatcca gctcgaagcc ctgtttcagc agttttgccg ccaggtccac
    53821 cacgcgttct ttatcgcctt cgcgcacgga aagcagcgca cgaccgtgtt tcttcatggt
    53881 ggagttgctg cccagctgcg ctttggcgaa cgcttcagcg aaggtgcggc ccacgcccat
    53941 gacttccccg gtagagcgca tttctggccc taacagcggg tcaacgcctg ggaatttatt
    54001 gaacggcagc accacttctt tcaccgagta gtacggcgga ataacttctt tggttacgcc
    54061 ctgctcagcc agcgatttgc cagccatcac acgcgccgcc acttttgcca gcggtacgcc
    54121 ggtggctttg gagacgaacg gaacagtacg cgccgcacgc gggtttactt caatcaggta
    54181 aacttcgttg tttttcaccg caaactgcac gttcatcaga ccgcgcacct gcaattcgaa
    54241 ggccagtttc tgcacctgct ggcgcatcac atcctgaatt tcctgactta aggtgtacgc
    54301 tggcagcgaa catgcggagt caccggagtg cacacccgcc tgctcgatgt gctccatgat
    54361 gccgccaatc agcaccattt cgccgtcgca gatggcatcc acgtcaactt ctactgcgtc
    54421 atcaaggaaa tgatccagca acactggcgc atcgttagac acgctaaccg ccgtctggaa
    54481 gtagcgacgc aggtcagctt cgtcatagac gatttccatc gcccgaccgc cgagaacgta
    54541 agacggacgc accaccagcg ggtagccaat ctcttttgcc ttctcaactg ccatttcaat
    54601 agcagtaacg gtggcgttcg ccggttgttt cagtttcaga cggtcaaccg catgctggaa
    54661 gcgttcacgg tcttccgcac ggtcgatagc atccgggctg gtgccgataa ccggtacgcc
    54721 agcggcttcc agtgcacgcg ccagtttcag cggggtctga ccgccgtact gaacgataac
    54781 gcctttcggc ttctcaatac gcacgatttc cagcacatct tccagagtta ccggctcgaa
    54841 gtagaggcga tcggaagtgt cgtagtcggt agagacggtt tccgggttac agttaaccat
    54901 aatggtttcg taaccgtctt cacgcagcgc cagcgaggcg tgtacgcagc agtagtcgaa
    54961 ttcgataccc tgaccgatac ggtttggacc gccgccaagc accatgattt tttcacggtc
    55021 ggtagacgga ttcgcttcgc actcttcttc ataagtggag tacatgtaag cggtgtcggt
    55081 ggcaaactct gccgcacagg tatccacgcg cttgtagacc gggtgcaggt catattggtc
    55141 acgcagctta cggatttccg cttcgcgtac gcccgccagt tttgccaagc gcgcatcggc
    55201 aaagcctttg cgtttcagct ggcgcaggaa ttcagcgttc aggccagtga tgcccacttc
    55261 cgccactttc tcttccagac gcaccagttc ttcaatctgt accaggaacc agcggtcaat
    55321 gttagtcagg ttgaagacgc cgtccacaga caggcccgcg cggaaagcat cggcgatgta
    55381 ccagatacgc tctgcgcctg cgtctttcag ttcgcgacgg attttggtta acgcttccgg
    55441 gtcatccagg ctcactttcg ggtcgaagcc agttgcgccc acttccaggc cgcgcagcgc
    55501 tttttgcagg gattcctgct gcgtgcgacc aatcgccatc acttcgccaa ccgatttcat
    55561 ctgagtggtc agacggtcgt tagcaccggc gaatttttcg aagttgaagc gaggaatttt
    55621 agtaaccaca tagtcgatgg acggctcgaa ggaggccgga gtacgtccgc cagtgatgtc
    55681 gttcatcagt tcgtctaggg tgtaacccac cgccagtttc gccgccactt tagcaatcgg
    55741 gaaaccggtc gctttcgacg ccagcgccga agaacgggac acgcgtgggt tcatttcgat
    55801 aacaatcagg cgaccgtttt tcgggttcac cgcaaactgg acattggaac caccggtttc
    55861 aacgccgatt tcacgcagca ccgccatcga ggcgttacgc atgatttgat attctttgtc
    55921 ggtcagcgtt tgggctggcg cgacagtgat ggagtcaccg gtgtggatgc ccatcgcatc
    55981 aaagttttcg atagagcaga cgatgatgca gttgtcgttt ttatcacgca ccacttccat
    56041 ctcgtactct ttccagccga tcagcgactc atcaatcagc aactctttgg tcggagagag
    56101 atccagaccg cgggcgcaaa tttcttcaaa ctcttcacgg ttataagcga taccgccgcc
    56161 gctaccgccc atggtaaagg atgggcgaat aatgcacggg aagcccacgt cagcggcaac
    56221 agccagcgct tcttccatcg tgtgtgcgat accggaacgc gcggtttcca gaccaatttt
    56281 cttcatcgct acgtcgaaac gacggcggtc ttctgcttta tcaatcgcat cggcagtggc
    56341 accaatcatg gtgacaccga actcttccaa cacgccctga cgttccagct ccagcgcgca
    56401 gttcagcgcc gtctgaccgc ccatggttgg cagcaccgcg tccgggcgct ctttttcaat
    56461 aatcttgcgt accacttccc agtgaatcgg ctcgatgtag gtcgcatcgg ccatttccgg
    56521 gtcggtcatg atggtcgccg ggttggagtt caccagaatg acgcggtaac cctcttcgcg
    56581 cagggcttta catgcttgcg cgccagagta gtcaaactca cacgcctgac cgataacaat
    56641 cgggcccgca cccagaatca ggatactttt tatatctgta cgttttggca tggctctttt
    56701 actcctgatt acttagcggt tttacggtac tgctcaatta acttgataaa gtggtcgaac
    56761 aacggcgcgg cgtcgtgtgg accagggctg gcttcagggt gaccctggaa gctgaacgcc
    56821 ggcttatcga tgcgatgaat gccctgtaac gtaccgtcga acagggattt atgcgtgaca
    56881 cgcaggtttg caggtaatgt tgcttcatcc accgcaaaac cgtggttctg ggcggtgatc
    56941 atcaccacgt ttttctccac atctttaacc ggatggttgc cgccgtggtg accaaatttc
    57001 attttgacag tcttcgcacc gctcgccagc gccagcagct gatgaccgag acagatgccg
    57061 aataccggaa tatcggtttc gaggaacttc tggatggcgg taatggcgta atcgcacggg
    57121 gccgggtcgc caggaccgtt ggagaggaag atgccgtctg gattcatttt cagcacatct
    57181 tccgcagaag tttgcgccgg aacgatggtc aggcgacagc ctctatccac cagcatccgc
    57241 aggatgttgc gcttggcacc aaaatcataa gccacgacgt ggaacggcag ctcgtcttct
    57301 tttttcgctt ctggcaggcc gccggtcaac gtccagctcc cttgtgtcca gctataggct
    57361 tctgcggtgg tcacttcttt tgccagatcc atgccgttca gacctgggaa cgcgcgggct
    57421 ttttctaacg ccagcgccgc atccgggtta tcgcctgcga taatgcagcc attctgtgcg
    57481 cctttctcgc gcagtaaacg cgtcagctta cgggtatcga tatcggcaat cgccacgatg
    57541 ttatggcgct tcaggtaaga agagaggtct tcggtattac ggaagttgct ggcaatcagc
    57601 ggcaggtcgc gaatcaccag accttgtgca tgtacctgag aagattcttc atcggcgtca
    57661 ttagtgccga cattgccaat atggggataa gtaagagtaa cgatttgacg agaataggaa
    57721 ggatcagtga ggatttcttg ataaccggtc attgaagtat tgaaaacgac ttccccaacc
    57781 gccgaacctg ttgcccctat ggcccgaccg tgaaactggg ttccgtcttc cagaaccaat
    57841 agcgctgact taatcaaaac accctccaga gaatactcac tcactttatt tgcatattaa
    57901 ttcacagtga tgacgtaaat caatgcaaat ctgcttaccg acgaatttct ggcaaacggc
    57961 ggcattctgg agatatgggc gctaaaagtc aacttaatgg caggattttt tatattattt
    58021 tgtggctcat gcatgtttta caggcattaa gcgacaaaaa catcatctaa cctgttctgg
    58081 aaaacgcttg cgcagcaaga agattgcatg aaataaaaac gagcaggaaa aagtggacca
    58141 aatggtcaaa atttacatta aaacaacaaa aatagaagac gaaacatgta atttttggca
    58201 gttatagaaa atttaaaagc ataacaccaa aaataaaagg actaaaaatt aaagcccttt
    58261 ataattaata aattaaatgt gttacttttg catcataata aacattttgt ggttacaaat
    58321 tattgagatc aagcacatct cgcatatcaa aaagaccact ttccttacca ctcaaccaca
    58381 aagccgatct taccgcaccg ttagcaaatg tcatgcggct ggacgcctta tgggtgatct
    58441 ccagacgctc gccaatatcg gcaaacatcg cggtatgttc gccaacgatg tcacctgcac
    58501 gcacggtagc aaaaccaatg gtacctggca cacgttcacc ggtatggcct tcacgactgt
    58561 agaccgcgca atctttcaga tctttatcaa gggcgtgggc gatcgcctct cccattgcca
    58621 gtgcggtgcc tgacggcgca tcaactttat gtctatgatg tgcttcaata atttcgatat
    58681 cggtgtagtc acccatcact ttggctgctt tctccagcag cttaagcatg acgttaacgc
    58741 caacgctaaa attggcggca aagacaatcg caatatcggc ggcggcgtca cgaattgcct
    58801 gtttaccggc ttcgtcaaac cccgtagtgc cgatcaccat ccctttgcca tgctgccgac
    58861 aaaaagcgag atggttcagc gtaccttccg gacgggtaaa atcgataaac acatcaaaat
    58921 catcttttat cgcatcaaga ctgctttgca cggtaacgcc tgttttccct gcaccagcca
    58981 gctcaccggc gtcgctgccc agtaaagaag atccttcgcg ctccagcgcc gcgcccagct
    59041 gcacgccttc taatgccagt gccgcctgaa tcaactggcg gcccatacgc cccccggctc
    59101 ccgcgatggc aacgcggatg tttgcatcat gcatagctat tctcttttgt taatttgcat
    59161 agaccgtttt cagagtaacc agcgcatcgc tacgccgcca gccaaaaacg ctgataatta
    59221 gaaattaatg ataaacaggg agggatatca gtgaaaggca tgagtcgatg aatgactgca
    59281 tgccgataac atgtgacagg ttacggcacc agagccagca cctcagccac ccactgttgg
    59341 aagcctttta cattcagatc caatgccacc tgtacattgg ctggtttgcc caggcaaccg
    59401 tcgatatcaa ccaccgtcgt gcctgaggta aattcgcctt gagtttctac cgcgacaaaa
    59461 cacggcttga gggtgaataa gtccgggcgc accagccagg cgatggcgca gagatcgtgc
    59521 attcgcagac cgctttgcat actgccgcta cggtagtggc taaacagggc gtggagcatt
    59581 tttccggtgc gattcaactc cggtaaagta gcgagatagt caggggttaa tattgcctga
    59641 ttggtgacat ccagaccgca catgacaatt tcgatgccgc tacggaagac acaggcagcc
    59701 gcttctggat cggcagcgat attaaactcg gcatttggtg tacagttgcc gcgaccagca
    59761 gaaccgccca tgatcaccag acgacgaata tacggtttac attccgggca ttgtaaaagt
    59821 aacagcgcaa tattggttaa cgggccgatg gccaccaggg taacgggctc tggtgcacgc
    59881 atcagggcat cccgaatcgc cagaaacgcc ggtatcccga gtggctttcg gttgtgctca
    59941 acaaagtcgt agccagccat tcccgattcg ccgtgcacag atgctgcatc acgcggtgcg
    60001 cgtaccagcg gcacagcggc tccctgggcg agcggaatct ccgcattcca gaaatgcagc
    60061 agttgcaggg cattgcgggt agttttctca accgagacat tacccgcgac ggtggtcatc
    60121 agttgcaggt cgagttcggg ggcaaaaatc gcggcggcaa tggcgacggc atcgtcaatg
    60181 ccggggtcag tatcgaggaa gataggtaaa cgcatgtttt cttcataaaa aatgccggta
    60241 acaagaccgg cattttcgca taacttaggc tgctaatgac ttaatcgact tcacgaatat
    60301 cgacacgcag ctctttcggc acttcgaaaa caatattttc ttcacggcct tccagcggaa
    60361 tggcttcacc accacccaac tgctgcaaac gtgccaccac attctgcacc agaatatccg
    60421 gagccgatgc gcccgcagtc acgccgacgc atttaacctc tttcacccac tcttcctgga
    60481 tatctttcgc atcgtcaatc aaaaacgcgt gtttgcccat acgctgggcc agctccgcca
    60541 gacggttgga gttggaggag tttttcgaac cgaccaccaa cacaacttcc gcctgttctg
    60601 ccagggcgcg taccgcttcc tgacggttag tcgtggcgta gcagatgtca tctttgcgcg
    60661 gaccgacaat tttcgggaag cgtttacgca gcgcgtcgat cacatcagac gtgtcatcca
    60721 ccgacagcgt ggtttgggtc ataaaggaga gcttctcttc gtttttgacc gtcagtttcc
    60781 acacatcgtc tggcgattcg actagataca ttcccccttc ggggttgctg tactgcccca
    60841 tcgtcccttc cacttccggg tgcccggcgt ggccgatgag aatagattct tcgccacggc
    60901 gactggcgcg ggcaacttcc atatgcactt tggtcaccag cggacaggtg gcatcaaaca
    60961 ccgtcaaatc gcggcttttc gcttcgttac gaaccgcctg agaaacaccg tgtgcggaga
    61021 aaatcaggat cgcgccgtcc ggcacttcgc taatctgctc aataaagata gccccacgct
    61081 cgcgcaagct atcgaccacg tagcggttat gcaccacttc gtgacggaca tatatcggtg
    61141 cgccgtaaat ggccagcgcg ttttcaacaa tgctgatagc gcggtctacc ccggcacaaa
    61201 aaccacgcgg gttggccaac aggatctgca tgttacgcct ccagtgccgg atcgatttcc
    61261 agcacttcaa tatcaaaatg aacggtctgc ccggccagcg gatggttgaa atcaacggta
    61321 atggagtcgc cgttaatttc gcggatcaca ccaggcatct cactgccatc cattgcggta
    61381 aaaagcatga ttgcgccaat ttctggctcg cctgcatcca taaattcacg gcgggagaag
    61441 tactgaatca ggtccggtga cggcacgcca aacgccgcat ctggctccag cgagaaggtg
    61501 gttttgtcgc ccactttcag ccccagcagg tgttgctcca gcccttcaga aagagaagca
    61561 tcgcccaggc ggaacagcgc cggtttaccg ttgttgcgag tagactcggc ggtggtgcca
    61621 tcgtcgagtt ttagcgtgaa gtgcaccagg acggcgctat tgctctgtac agattcagac
    61681 atgcaggttt gctcttattt ttgaggattt ttgccggatg cggcctacga tttcgtagca
    61741 atctgtaggc cgcatccggc aggatttatt attgtttttt cgctttggaa ggtaaaaacc
    61801 cttccagcac gatcagtgcc gcaccgacac aaatggcagt atcggcaagg ttgaaggtgg
    61861 cgaagtgcca gtcgccgacg tagaagtcga tcatatcgac aacgaagccg tgccacaggc
    61921 ggtcgaacag gttgcccagc gcgccgccaa taatcagcgc gtaagcgata ttgtttagct
    61981 tctgcgtggc cttcgagcga tacatcatca ctgccaggag cacgctaata ccaatcgcaa
    62041 taccggcaaa gaaccaacgc tgccagccgc cgctatcggc aaggaaacta aacgccgcgc
    62101 catagttacg cgcataatgc agattaagcg acgggaacag cgggaccgta tcccccagag
    62161 caaagttctg gaggatcagg tatttgctgc ccagatcgat aatcagcacg actaccacca
    62221 gccacagcca gcgtagccct gttgaacaga tcgattgact catcaggcaa acttacgttt
    62281 ttcaccgtca ccggcgacgt tgctgacaca gcggccgcag atttctgcgt gttccgccac
    62341 cttgccgaca tcctgggtgt agtgccagca gcgtgggcac ttctcacctt cggctttact
    62401 caacgcgact ttcagccctt tgagtacttc gctctgctga gcatcagcag gtgcgtcgtt
    62461 atagtctgca acggtagcgc cggaggtcaa caggacaaat cgtaattcat cgcccagcgc
    62521 ggtcagtttc gccgccagtt ccggttctgc atacaaggtt actgccgctt ccagcgagcc
    62581 acccactttc ttgtcggcac gcgcttgctc aatgactttg ttcacttcgc cacgcacttt
    62641 caacagctcg tcccagaacg catcgttcat tgcttcactg tctgccagac caaacaggcc
    62701 ttcgtaccac tcaccggtga agacgtattt ttcacgttcg cccggcagat agccccacac
    62761 ttcatcagcg gtgaaggaga ggattggtgc catccagcgc accagcgctt cagcgatgtg
    62821 atacagcgca gtctggcagc tacgacgcgc cacgctatcc gctttggcgg tgtactgacg
    62881 gtctttgatg atgtcgaggt agaaggaacc catctcaacg gagcagaagc gcatcagacg
    62941 ctgtaccact tcgtggaaat cgtatgcttc gtacgccttg aggatgtctt cctgtgccgc
    63001 tttcgcacaa cctacggccc agcgatccag taccaccatc tcttccggtt tcaccatatc
    63061 ttttgctgga tcaaaaccat tcaggtttgc cagcaggaag cgcgcggtgt tacggatacg
    63121 acgatagcta tcggcagcac gtttcaggat ctcgtcagaa acggccattt caccggtgta
    63181 gtcggttgat gccacccaca gacgcagaat atccgcgccc agtttgttca tcacatcctg
    63241 cggcgaaacg gtgttgccga tggatttaga catcttgcga ccctgaccat ccacggtaaa
    63301 gccgtgggtc agtacctgac gatacggcgc tttgcctttc atcgcggtgg agatcattag
    63361 ggaagacatg aaccaaccac ggtgctggtc agaaccttcc agatacatgt ccgctgcgtg
    63421 accggcaaat tccggacgca cgtcaacaac agaagagtgg gtagatccgg agtcaaacca
    63481 tacgtcgaga gtatccggca ctttcacata ctggtcagct tcgtcgccga ggatctcttt
    63541 cgcatcgaga tcccaccacg cctggatgcc gtcaacttca acgcgtttag ccacttcttc
    63601 catcagttcg agggtacgcg gatgcagctc ttccgtgtct ttgtgcacga acagtgacat
    63661 cggtacgccc caggtgcgct gacgggagat acaccagtca ggacggttag caaccatcga
    63721 ctcgatacgc gcctggcccc agtccgggag ccactgcacg cctttgatct ctttcagtga
    63781 ctgcgcacgc agacctttct gatccatgct gacgaaccac tgcggtgtcg cgcggaagat
    63841 gatcggcgtt ttgtgacgcc agcagcacgg atagctgtgc tgcattttct caacgtgcag
    63901 cagcgcgcct ttttcctgca gcagcgcaac gacgatgtcg ttcgctttga agacgttcac
    63961 gccatccagc gtcggataag tgcccggcag ataagtgccg tccgggccaa ccgggttagc
    64021 ggtttccagg ccgtatttct gaccgatcac atagtcgtcc gggccgtggc caggcgcggt
    64081 gtgaacggca ccggtaccgg catccagggt aacgtgatcg ccgaggattg ccggaacgtc
    64141 gaagcccata aacggatggg taaagcgcag cagctcaagc tctgcacctt ttaccgtgcc
    64201 gagaatggtg taatcggtca cgccgatacg ctgcattacg ctttcaacca gatctttcgc
    64261 cagaatcacg gcctgaccgt cgatctgcac cagcgcatag tcgaaatcag gtgcaataga
    64321 gattgcgcgg ttcgcaggca gagtccacgg cgtagtggtc cagatcacca gcgagattgg
    64381 gccgttaacg ttgcttacgc caaatttcgc tttcagcgca tcctgatcga ccgcctggaa
    64441 agcgacgtca atggacggag aagttttgtc gtaatactca acttccgctt ctgccagtgc
    64501 agaacggcag tctacgcacc agtgcaccgg cttcgcgcct ttgtgcaggt gaccattgcc
    64561 gatgattttg cccagcgcgc ggatgatgtt ggcttcagtt ttgaagtcca tagtcagata
    64621 cgggtgcgac cagtcgccca gcacgcccag acggataaag tctttgcgtt gaccgtcaac
    64681 ctgggtcgcc gcgtattcgc ggcacttggc gcggaactcg gcggcggtga atttctcacc
    64741 cggcttaccg tattcttgct ctactttcag ctcgatcggc agaccgtggc agtcccagcc
    64801 aggcacatac ggcgagtcat aaccggaaag ccctttggac ttcacgataa tgtctttcag
    64861 aatcttgtta accgagtgac caatatgaat gctgccattc gcataaggag ggccatcatg
    64921 cagaatgaag gtttttttgc cttttttagc cgcacggatg atgccgtaca gatcatcatc
    64981 agtccaacgc gccagcattc ccggttcgcg cttggcgaga tcgccacgca tcgggaaccc
    65041 tgtttccggc aaattcaggg ttgatttata gtcactcatc agattctcgg ttccgtgttt
    65101 cggtttgatt acataacagg cttaagccgg ttttgttagc ccaaaaaatt cgcgggcggt
    65161 taattcatca cgcgcaatct gcgctttcag ttcgtccagc gacgcaaatc gctgctcatt
    65221 gcgtattttt ttacgcagca ctacttgtat atggcgaccg taaaggtcca ttgcaacatc
    65281 taacaaatgc acttccagct gctggcgaat accggcaacc gttgggcgtg ttccgatgtt
    65341 tgccacgccg ggtaacggct tttcaccgag gcccagcact tctaccgcat aaaccccttt
    65401 caccggggaa acctgacggc gtagcggtac attcgccgtc gggaaaccta tagtgcgccc
    65461 taattcatca ccgtggacta cacgcccgga gatggcaaac gggtgcccca gtaaactttc
    65521 tgccagagcc agattgtcat cagccagcgc ctgacgcacg gcggtgctgc tgatacgcac
    65581 accaccttcg caaaaagttt gcgtactggt gatatcgaag ccgtattcca tgccagcttt
    65641 ctgtaataac aagaaatcgc cttcacgacc agcgccaaag cggaaatcat cacctacggc
    65701 aagaaatttt acgcgcaaat gcttcaccaa aagatcgctg atgaaatttt gcgcggttaa
    65761 cgccgcaaaa cgcctgtcga aacgcacgca cagcacgtaa tcaacgccac actctgcaag
    65821 gtaacgcagt ttttcccgca gccgggtcag tcttgccggg gctttatcgg tagcaaacag
    65881 ttccagtggt tgaggttcaa aaagcatcac catcaccggt aagttgcgct tgcgcccttc
    65941 ttcctgcaag ccctgtaaca gcgcgcgatg accgcgatgc acgccgtcga aattaccaat
    66001 agtcagcaca cacccttctt gcggggcctg gctgagatta tgtatgccgc gtatcagctt
    66061 catgtctggc tcaaaacagt gaaaatcgtc cgagtatacc ttgtacagcg gtcaaggtta
    66121 accggcgatt gagtaccgaa atcagaaaga agtggctttt cattgccatg gagcaaatca
    66181 cgggaagaaa ctgaccgcct gctgcaattt ttatcgcgga aaagctgtat tcacaccccg
    66241 caagctggta gaatcctgcg ccatcactac gtaacgagtg ccggcacatt aacggcgctt
    66301 atttgcacaa atccattgac aaaagaaggc taaaagggca tattcctcgg cctttgaatt
    66361 gtccatatag aacacatttg ggagttggac cttggctaat atcaaatcag ctaagaagcg
    66421 cgccattcag tctgaaaagg ctcgtaagca caacgcaagc cgtcgctcta tgatgcgtac
    66481 tttcatcaag aaagtatacg cagctatcga agctggcgac aaagctgctg cacagaaagc
    66541 atttaacgaa atgcaaccga tcgtggaccg tcaggctgct aaaggtctga tccacaaaaa
    66601 caaagctgca cgtcataagg ctaacctgac tgcacagatc aacaaactgg cttaatcgcc
    66661 aatttgctga agctttgtga aaaagcccgc gcaagcgggt ttttttatgc ctgctgcttt
    66721 tgtgatgcgt gagagaacac actgccggtt gccagatagt caaaagagac atgccgttgt
    66781 actatctgaa ctcttcgtgg cttaagaatc gtagaaccat cggctgatac attctgaaca
    66841 agaatatcta ctcaatctcc agactccccc acaaagaata tggatattgt gatacacatt
    66901 gaggtattaa acttaagacc actctatttt tgaattaaat tcctatatac tttaaccatt
    66961 cataaatatc tcctcattag accttgt
//
LOCUS       DAETQL010000009        66034 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.009, whole genome shotgun sequence.
ACCESSION   DAETQL010000009 DAETQL010000000
VERSION     DAETQL010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.009"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(130..609)
                     /gene="folK"
                     /locus_tag="K0W65_000619"
     CDS             complement(130..609)
                     /gene="folK"
                     /locus_tag="K0W65_000619"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBI3094012.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(606..2024)
                     /gene="pcnB"
                     /locus_tag="K0W65_000620"
     CDS             complement(606..2024)
                     /gene="pcnB"
                     /locus_tag="K0W65_000620"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBI3094013.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2063..2989)
                     /gene="gluQRS"
                     /locus_tag="K0W65_000621"
     CDS             complement(2063..2989)
                     /gene="gluQRS"
                     /locus_tag="K0W65_000621"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBI3094014.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3026..3481)
                     /gene="dksA"
                     /locus_tag="K0W65_000622"
     CDS             complement(3026..3481)
                     /gene="dksA"
                     /locus_tag="K0W65_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HBI3094015.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3659..4363)
                     /gene="sfsA"
                     /locus_tag="K0W65_000623"
     CDS             complement(3659..4363)
                     /gene="sfsA"
                     /locus_tag="K0W65_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBI3094016.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4378..4908)
                     /gene="thpR"
                     /locus_tag="K0W65_000624"
     CDS             complement(4378..4908)
                     /gene="thpR"
                     /locus_tag="K0W65_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBI3094017.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4982..7411
                     /gene="hrpB"
                     /locus_tag="K0W65_000625"
     CDS             4982..7411
                     /gene="hrpB"
                     /locus_tag="K0W65_000625"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBI3094018.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7607..10141
                     /gene="mrcB"
                     /locus_tag="K0W65_000626"
     CDS             7607..10141
                     /gene="mrcB"
                     /locus_tag="K0W65_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBI3094019.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10361..12604
                     /gene="fhuA"
                     /locus_tag="K0W65_000627"
     CDS             10361..12604
                     /gene="fhuA"
                     /locus_tag="K0W65_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBI3094020.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTNKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12655..13452
                     /gene="fhuC"
                     /locus_tag="K0W65_000628"
     CDS             12655..13452
                     /gene="fhuC"
                     /locus_tag="K0W65_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBI3094021.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13452..14342
                     /gene="fhuD"
                     /locus_tag="K0W65_000629"
     CDS             13452..14342
                     /gene="fhuD"
                     /locus_tag="K0W65_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBI3094022.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14339..16321
                     /gene="fhuB"
                     /locus_tag="K0W65_000630"
     CDS             14339..16321
                     /gene="fhuB"
                     /locus_tag="K0W65_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBI3094023.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16356..17636)
                     /gene="hemL"
                     /locus_tag="K0W65_000631"
     CDS             complement(16356..17636)
                     /gene="hemL"
                     /locus_tag="K0W65_000631"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBI3094024.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17861..19282
                     /gene="clcA"
                     /locus_tag="K0W65_000632"
     CDS             17861..19282
                     /gene="clcA"
                     /locus_tag="K0W65_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBI3094025.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19306..19371
                     /locus_tag="K0W65_000633"
     CDS             19306..19371
                     /locus_tag="K0W65_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094026.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19364..19708
                     /gene="erpA"
                     /locus_tag="K0W65_000634"
     CDS             19364..19708
                     /gene="erpA"
                     /locus_tag="K0W65_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBI3094027.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19755..20378)
                     /locus_tag="K0W65_000635"
     CDS             complement(19755..20378)
                     /locus_tag="K0W65_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HBI3094028.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20416..21216)
                     /gene="btuF"
                     /locus_tag="K0W65_000636"
     CDS             complement(20416..21216)
                     /gene="btuF"
                     /locus_tag="K0W65_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBI3094029.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNLVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21209..21907)
                     /gene="mtnN"
                     /locus_tag="K0W65_000637"
     CDS             complement(21209..21907)
                     /gene="mtnN"
                     /locus_tag="K0W65_000637"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBI3094030.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21991..23508
                     /gene="dgt"
                     /locus_tag="K0W65_000638"
     CDS             21991..23508
                     /gene="dgt"
                     /locus_tag="K0W65_000638"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBI3094031.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23638..25062
                     /gene="degP"
                     /locus_tag="K0W65_000639"
     CDS             23638..25062
                     /gene="degP"
                     /locus_tag="K0W65_000639"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBI3094032.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25217..26373
                     /gene="cdaR"
                     /locus_tag="K0W65_000640"
                     /pseudo
     CDS             25217..26373
                     /gene="cdaR"
                     /locus_tag="K0W65_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26462..26848)
                     /locus_tag="K0W65_000641"
     CDS             complement(26462..26848)
                     /locus_tag="K0W65_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBI3094033.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27162..27986)
                     /gene="dapD"
                     /locus_tag="K0W65_000642"
     CDS             complement(27162..27986)
                     /gene="dapD"
                     /locus_tag="K0W65_000642"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBI3094034.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQATPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28017..30689)
                     /gene="glnD"
                     /locus_tag="K0W65_000643"
     CDS             complement(28017..30689)
                     /gene="glnD"
                     /locus_tag="K0W65_000643"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBI3094035.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30751..31545)
                     /gene="map"
                     /locus_tag="K0W65_000644"
     CDS             complement(30751..31545)
                     /gene="map"
                     /locus_tag="K0W65_000644"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBI3094036.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31913..32638
                     /gene="rpsB"
                     /locus_tag="K0W65_000645"
     CDS             31913..32638
                     /gene="rpsB"
                     /locus_tag="K0W65_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBI3094037.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32896..33747
                     /gene="tsf"
                     /locus_tag="K0W65_000646"
     CDS             32896..33747
                     /gene="tsf"
                     /locus_tag="K0W65_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBI3094038.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33894..34619
                     /gene="pyrH"
                     /locus_tag="K0W65_000647"
     CDS             33894..34619
                     /gene="pyrH"
                     /locus_tag="K0W65_000647"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBI3094039.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34911..35468
                     /gene="frr"
                     /locus_tag="K0W65_000648"
     CDS             34911..35468
                     /gene="frr"
                     /locus_tag="K0W65_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBI3094040.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35560..36756
                     /gene="ispC"
                     /locus_tag="K0W65_000649"
     CDS             35560..36756
                     /gene="ispC"
                     /locus_tag="K0W65_000649"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBI3094041.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36945..37703
                     /gene="ispU"
                     /locus_tag="K0W65_000650"
                     /gene_synonym="uppS"
     CDS             36945..37703
                     /gene="ispU"
                     /locus_tag="K0W65_000650"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBI3094042.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37716..38573
                     /gene="cdsA"
                     /locus_tag="K0W65_000651"
     CDS             37716..38573
                     /gene="cdsA"
                     /locus_tag="K0W65_000651"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBI3094043.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38585..39937
                     /gene="rseP"
                     /locus_tag="K0W65_000652"
     CDS             38585..39937
                     /gene="rseP"
                     /locus_tag="K0W65_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBI3094044.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39967..42399
                     /gene="bamA"
                     /locus_tag="K0W65_000653"
     CDS             39967..42399
                     /gene="bamA"
                     /locus_tag="K0W65_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBI3094045.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42521..43006
                     /gene="skp"
                     /locus_tag="K0W65_000654"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42521..43006
                     /gene="skp"
                     /locus_tag="K0W65_000654"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBI3094046.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43010..44035
                     /gene="lpxD"
                     /locus_tag="K0W65_000655"
     CDS             43010..44035
                     /gene="lpxD"
                     /locus_tag="K0W65_000655"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBI3094047.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44140..44595
                     /gene="fabZ"
                     /locus_tag="K0W65_000656"
     CDS             44140..44595
                     /gene="fabZ"
                     /locus_tag="K0W65_000656"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBI3094048.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44599..45387
                     /gene="lpxA"
                     /locus_tag="K0W65_000657"
     CDS             44599..45387
                     /gene="lpxA"
                     /locus_tag="K0W65_000657"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBI3094049.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45387..46535
                     /gene="lpxB"
                     /locus_tag="K0W65_000658"
     CDS             45387..46535
                     /gene="lpxB"
                     /locus_tag="K0W65_000658"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBI3094050.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46532..47128
                     /gene="rnhB"
                     /locus_tag="K0W65_000659"
     CDS             46532..47128
                     /gene="rnhB"
                     /locus_tag="K0W65_000659"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBI3094051.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47165..50647
                     /gene="dnaE"
                     /locus_tag="K0W65_000660"
     CDS             47165..50647
                     /gene="dnaE"
                     /locus_tag="K0W65_000660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBI3094052.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50660..51619
                     /gene="accA"
                     /locus_tag="K0W65_000661"
     CDS             50660..51619
                     /gene="accA"
                     /locus_tag="K0W65_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBI3094053.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51718..53859
                     /gene="ldcC"
                     /locus_tag="K0W65_000662"
     CDS             51718..53859
                     /gene="ldcC"
                     /locus_tag="K0W65_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBI3094054.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIAPEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53916..54305
                     /locus_tag="K0W65_000663"
     CDS             53916..54305
                     /locus_tag="K0W65_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBI3094055.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54370..55668
                     /gene="tilS"
                     /locus_tag="K0W65_000664"
     CDS             54370..55668
                     /gene="tilS"
                     /locus_tag="K0W65_000664"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBI3094056.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55717..55977)
                     /gene="rof"
                     /locus_tag="K0W65_000665"
     CDS             complement(55717..55977)
                     /gene="rof"
                     /locus_tag="K0W65_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBI3094057.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55964..56164)
                     /locus_tag="K0W65_000666"
     CDS             complement(55964..56164)
                     /locus_tag="K0W65_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBI3094058.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56330..56875
                     /locus_tag="K0W65_000667"
     CDS             56330..56875
                     /locus_tag="K0W65_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBI3094059.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56872..57294
                     /gene="arfB"
                     /locus_tag="K0W65_000668"
     CDS             56872..57294
                     /gene="arfB"
                     /locus_tag="K0W65_000668"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBI3094060.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57308..58018
                     /gene="nlpE"
                     /locus_tag="K0W65_000669"
                     /gene_synonym="cutF"
     CDS             57308..58018
                     /gene="nlpE"
                     /locus_tag="K0W65_000669"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBI3094061.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58173..58997)
                     /locus_tag="K0W65_000670"
     CDS             complement(58173..58997)
                     /locus_tag="K0W65_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HBI3094062.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59050..60768)
                     /gene="proS"
                     /locus_tag="K0W65_000671"
     CDS             complement(59050..60768)
                     /gene="proS"
                     /locus_tag="K0W65_000671"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBI3094063.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60879..61586)
                     /gene="tsaA"
                     /locus_tag="K0W65_000672"
     CDS             complement(60879..61586)
                     /gene="tsaA"
                     /locus_tag="K0W65_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBI3094064.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61583..61987)
                     /gene="rcsF"
                     /locus_tag="K0W65_000673"
     CDS             complement(61583..61987)
                     /gene="rcsF"
                     /locus_tag="K0W65_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBI3094065.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62105..62920)
                     /gene="metQ"
                     /locus_tag="K0W65_000674"
     CDS             complement(62105..62920)
                     /gene="metQ"
                     /locus_tag="K0W65_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBI3094066.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62960..63613)
                     /gene="metI"
                     /locus_tag="K0W65_000675"
     CDS             complement(62960..63613)
                     /gene="metI"
                     /locus_tag="K0W65_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBI3094067.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63606..64637)
                     /gene="metN"
                     /locus_tag="K0W65_000676"
     CDS             complement(63606..64637)
                     /gene="metN"
                     /locus_tag="K0W65_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBI3094068.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64825..65400
                     /gene="gmhB"
                     /locus_tag="K0W65_000677"
     CDS             64825..65400
                     /gene="gmhB"
                     /locus_tag="K0W65_000677"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBI3094069.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65764..>66034
                     /locus_tag="K0W65_000678"
     rRNA            65764..>66034
                     /locus_tag="K0W65_000678"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15631 a        16348 c        18197 g        15858 t
ORIGIN      
        1 gatgggattg atatttatct gcgggaattt atattatgtt tctgcaaaaa tgtttaaact
       61 aaaattttta ttaaggaaag agaggatgta tgattttcag aaaatattta tattcgtaat
      121 ataaataaat taccatttgc ttaatttgtc aaatgctctt gtatgtaaga cttcacgcaa
      181 cgtctcccca tcaggaaacg ccagctccgg tgcaatctca aacagcggcc acagcataaa
      241 tccacgattc ttcatatcgt agtgcggaac ggtcaggcgt tcggtattta tcacttcatt
      301 accaaacagc atgatatcga gatccagcgt gcgtggtccc cagcgttcag ctttacgaac
      361 acgaccctgt tgcagttcta tgcgctgtgt atggttgagt aactcttccg gggcgggcga
      421 agtttcaagc gccacggcgg cgttgaggta atcgggttga tcttgcggcc ccagcggtgg
      481 ggtgcggtaa aacgaagaaa cggcaagaat gcggctttca gggatatcgc ctaatgcttt
      541 cagggcagca ttgacctgct ccagcggaga ggccagattg ctgcctatgg caatatacgc
      601 cactgtcatg tggtaccctc acgacgcggt gcgcgtttgc gtggacgacg agtacgacga
      661 cgcggtgacg gttcttcatc cagctcgtta agcatccctt tttgatctgg tggtgcggaa
      721 acctggaact caccccacca tttcaccaga cgctgcagtt cagcattacg ttcaacttca
      781 gctcgcaagg ccaacaggtc ataagccgca cggaacttag gatgctccag cagtttccat
      841 gcgcgtttac cctgacgacg ggacatacgc aactgcaact gccagatatc gcgggttaac
      901 gtcgtcagac gtttcgggat tgccagtgaa cggcaggctt cgtccagcac gtcgttcatc
      961 gccagcgcga aagcgtcgtg ataggtcagg ccgctttcct gggcgatctt ctgtgccgtc
     1021 tccagcagtg ggtaccagaa catggcggca aacaagaacg ccgggttcac gcgcatatcg
     1081 ttatggatac gcgtatcggt attcttcagc acctgttcaa tgatccgctc catcgggctg
     1141 tcgccatttt ccgtgaagta gcgggtaatg gtcgggaaca gcggctggaa cagatgatat
     1201 tcacacaaca gcttataggt ttcgtaaccg tagcccgctt gtagcagttt aagcgattct
     1261 tcaaacaggc gtgccggtgg gatatcgttc agcagggtag cgaggcgagg gatcggttct
     1321 gcggtttccg ggctgatgcg catacccaat ttggcggcaa aacgtaccgc gcgcagcata
     1381 cgtaccggat cttcacggta gcgcgtttcc gggttaccaa tcagacggat aacgccgtcc
     1441 ttcagatcct tcataccacc aacgtaatca cggacggtaa aatccgctac gctgtaatac
     1501 aggctgttga tagtgaaatc gcggcgctgg gcgtcttctt cgatggagcc gaaaatgttg
     1561 tcgcgcagca acatgccgtt ttgcccgcgt tgggaggtcg tgcggtcgct gacgttacct
     1621 tcgtggtgtc cacggaaggt cgcaacttcg ataatctccg ggccaaacat tacatgagcc
     1681 agacggaaac ggcgacccac caggcggcag ttacggaaca gtttgcgcac ctgctcaggc
     1741 gtggcgttag tggttacgtc aaaatctttc ggctttttgc caagtaacag gtcgcgcacg
     1801 ccgccgccaa ccagccaggc ttcgtatccc gctttgttga gcctgtacat taccttcagg
     1861 gcattttcac tgatatcttt gcgggaaata gcatgctgct cacgcgggat caccgtcacc
     1921 tgtggacggg cgactgcctg ttcagcctcg ctttcctcgc ggcttagcac cttgcggcaa
     1981 aaattagcga ctcgggtaaa aatagtacac ctcggtagtg tcaaacatca ttcaggacaa
     2041 aaaaatagcg gctaatcata gctcagcatg acgcatttga gaatgttgaa tttacaattg
     2101 ccgactcggg cacggcggtc agcatccagt ttttgacggc tgactgaagg atttgctcga
     2161 cgctgaaatc ctgccagtgt gcttctgcct gctgccccag aaattgaagt gccgcgatta
     2221 gtaccgggcg tggatcgcct ttcggcaacg caggcgcatg attctgcttg gaaagtttag
     2281 cgccttgtgg attaagcgcc agcggcagat gaatgtaatc tggcactttc cagccaaaaa
     2341 gctggtacag cgggatttgc cttactgttg gttcaatcag atcagcccca cgcactattt
     2401 ctgtaacgcc ctggaaatga tcatcaacca caacagccag gttgtaggcg aacaacccat
     2461 cacggcgatg aatgataaaa tcttcccgtg ccagtttttc gtcggcgtga ataatgcctc
     2521 gcagcaggtc agtaaattgc gtgaccggat gctgctggcg gatacgcact gcggcgttgt
     2581 ctggtccatg atgcaacacc cggcaatgac cgtcgtaaat accgccaatg ctttgaatac
     2641 gcgcacgcgt acaggtgcag taataactta gtccttgttc atgtaaccag gcgagtgctt
     2701 cacgataggc gtcgtgacgt tgcgattgcc agagaacatc gccgtcccag tgcagaccgt
     2761 aatgttccag ctggcgcagg atagtttctg cggcaccggg aacttcacga ggcgggtcga
     2821 tatcttctat gcgtaccagc cagcgacctt gccgggcgcg agcctgcaaa tagctgccga
     2881 gcgcggcgat cagagagcca aaatgaagct cgccggaagg agagggggcg aagcggccaa
     2941 tatactgtgt gtctgtcatc tctttgaaca aaaaataagg cgggagcatt tcccgcctgt
     3001 ggtaaacgtg atggaacggc tgtaattagc cagccatctg tttttcgcga atttcagcca
     3061 gcgttttgca gtcgatgcac agatcggctg tcgggcgcgc ttccagacgg cgaataccaa
     3121 tttcaacacc gcaggattcg cagtagccga aatcttcgtc ttccactttt ttcagcgtct
     3181 tctcgatctt tttgatcagc ttacgctcgc gatcgcggtt acgcagttcg aggctgaact
     3241 cttcttcctg ggctgcacgg tctaccgggt ccgggaagtt ggctgcttca tcctgcatat
     3301 gtgtaacggt gcgatcgact tcatccctga gttgattacg ccatgcttcc agaatacgac
     3361 ggaagtgcgc cagctgggct tcattcatat actcttcgcc cggcttctcc tgatatggtt
     3421 ccaccccagc gatggcgaga atactcaggg acgatgtttt acggttttgc ccttcttgca
     3481 tgttgcttct ccttaacacg cactatcgat ccccatgttc gggggaaaaa tgaggccgct
     3541 ataaatagca gatgcttttc cggatagcaa ttatctaaac gtaacacttg acaactgtgt
     3601 gaggaaaagc gtatttgcgc acgcgaccag aatgtaaatt aaccagttac ttactttact
     3661 acaatgtaac cggcagtgat tttttaagag ccatgccttc agcagaaatt tccgctttgt
     3721 aagccagaat ttctaccccc ctctgttgag cttctgacaa tagttgcgcg tatttctcat
     3781 cgatgtggcg cgcgggtgaa aaccgtgtaa tggctgaatg cagcacggcg aaaaaaataa
     3841 ccgcacgctg gccttcagcc gctacgctca tcaactcccg aaggtgtttc tgacctcgtt
     3901 cagtgaccgc atcgggaaaa tatccctgtt cgttctccgc taacgtaacc gatttcactt
     3961 caatatagca gtctggacgc gaatccgcct gcaacataaa gtcaatacgg ctgcgttcgg
     4021 agccgtattt tacttcgctt ttcagcgagc tatagcctga cagttctgaa attgattcat
     4081 taaggatagc ctctttcgtc aacctgttag cccaaagcgt attgacgcaa ataaatgcgc
     4141 cgctctggct ttgagttaat tcccaggtgt gtgggtattt ccgtttggtg ttgtctgaag
     4201 tcgaatacca gacggtatcg ccaggcgttg cacaaccggt catcgcaccc gtattcgggc
     4261 agtgtagcgt taattcgcga ccatcgggtg tgatcacatc ggctaaaaaa cgtttgtaac
     4321 gctgaattag cgtcgcgcgc tgtagagggg gagaaaattc cattgcgaca atccttgtta
     4381 ttgcgttagc gcccagcgtt ttagcggcgt gtagcgtgtg cgtccacggg caaacgagga
     4441 ggcgtaaagg gtgaactccg tcaccgcata agaccagtta aaacccggcg gcgggaatgt
     4501 caccgcctcg ctggcgtcgc gcaataaggt aatatgtgga tgaaacggac gattgctttg
     4561 aaaacaaccg ctgcgggcag cctgtgaacg gagcatattc gccagctgga ttaagccgcg
     4621 tggaggctga cgcatcccta accacaccac acgcgaacgc agccattgtc cggcgtcatc
     4681 aagcgtgagt gtgaaaccag gttgacgaat ccgtccggct aaaagagaaa gcgccttctc
     4741 tttctctgcg ctcacttcgc ctaaaaatgc cagagtcagg tgcaaattat cggcggcgac
     4801 cggacgtccc gcctcaggtg ggaagtgtgt ggcgcgccaa tggataatct gttcgcggat
     4861 ttctgcaggt aagtcgatag caaagaacag acgttgcggt tcagacatgt gaggcactcg
     4921 gttatgaatt accgcgatgc tacaatgtgg cgcgaagaat gttaaccctc tggagcgttt
     4981 tgtgtcgtcg ttgcccgttg ctgccgtctt acctgaatta cttaccgctc tcgattgtgc
     5041 gccgcaggta ttattaagtg cgccgaccgg ggccgggaaa tcaacctggc tgccgctgca
     5101 actgctggcg catcccggca ttaacgggaa aattatcctg ctggagccgc gtcgtctggc
     5161 ggcgcgtaac gtcgcgcaac ggctggcgga gctgcttaac gaaaagccag gcgataccgt
     5221 tggctaccgg atgcgtgcgc aaaactgcgt cgggctgaat acccgcctgg aagtggttac
     5281 cgaaggcgtg ctgacgcgga tgatccagcg tgacccggaa ctgagcggtg ttggattggt
     5341 gatccttgat gagtttcatg agcgcagttt gcaggcggat ttggcgttgg cgctgttact
     5401 cgatgtgcaa caaggtctgc gtgatgacct taaactgctg attatgtcgg ctacgctgga
     5461 caatgaccgc ttgcagcaaa tgctgccgga agcgcccgtc gtcatctcag aagggcgctc
     5521 gtttccggtt gaacgccgtt atttaccgct gcccgcgcat cagcgttttg acgaagccgt
     5581 tgcggtagcc accgccgaaa tggtgcgtca ggaaagtgga tcattactgt tatttttacc
     5641 tggcgtcgga gaaattcagc gcgtgcagga acaactggct tcgcgcatcg gcagtgatgt
     5701 ggtgctctgc ccgctgtatg gcgtgttgtc actgaacgat cagcgaaaag cgatcctccc
     5761 ggcaccgcaa gggatgcgca aagtggtgct ggcgaccaat attgctgaaa ccagtttaac
     5821 catcgaaggt attcgtctgg tggtggattg tgcccaggag cgtgtggcgc gctttgatcc
     5881 gcgcacgggg cttacgcggt tgattactca acgcgttagt caggcatcca tgacgcagcg
     5941 tgccgggcgc gccgggcgtc tggagccggg tatcagcctg catttaatcg ccaaagaaca
     6001 agcagaacgc gccgcggcgc aaagtgagcc ggagatctta caaagcgatc tttccggttt
     6061 gctgatggaa ttactgcaat ggggatgcag cgatccggcg cagatgagct ggctggatca
     6121 accgccaacg gtgaatctac tggccgcgaa acgcctgtta caaatgctgg gggcgctgga
     6181 gggtgaacgg cttagtgcgc aagggcaaaa aatggcagcg ctgggtaacg atccgcgttt
     6241 agcggcaatg ctggttaacg caaagagcga tgacgaagct gctaccgcgg caaaaattgc
     6301 cgccattctc gaagagccgc cacggatggg caatagtgac ctgggcgtgg cgttttcgcg
     6361 caatcaacca gcctggcagc aacgtagtca gcaactgtta aaacgcttaa acgtacgcgg
     6421 cggtgaggca gacagttcgc ttatcgcgcc gctacttgcc ggggcgtttg ccgatcgcat
     6481 tgctcatcgc cgtgggcaag atggacgcta tcaactggca aacagcatgg gagcgatgct
     6541 cgatgccgac gacgcgctaa gccgccacga atggttgatc gcaccgttat tattgcaggg
     6601 cagcgcctcg ccggatgcgc ggattttact ggcgctaccg gtcgatattg atgaattagt
     6661 acaacgctgt ccgcagctgg tgcagcaatc tgacaccgtg gaatgggatg acgcgcaagg
     6721 tacgctgaaa gcctggcgtc ggctacaaat cggtcagttg acggtgaaag tgcagccgct
     6781 ggcgaaaccg tcagaagacg agttgcatca ggcgatgctt aatggcatcc gtgataaagg
     6841 tttaagcgtg ctcaactgga cggcggaagc ggaacagcta cgcttgcgtt tgttatgcgc
     6901 cgcaaagtgg ttgccggaat atgactggcc agcggttgat gatgaaagtt tgttggcgac
     6961 gctggaaacg tggctgctgc cacatatgac cggcgtacat tcactacgcg gcctgaaatc
     7021 actcgacatt tatcaggcac tacgcggatt acttgattgg ggaatgcagc aacgtctgga
     7081 tagtgaattg cctgcgcatt acactgtgcc gacgggaagc cggatcgcca ttcgttatca
     7141 tgaagataac ccgcccgcgc tggcggtgag aatgcaggag atgtttggtg aggccactaa
     7201 tccgacgatc gcccaggggc gcgtgccgct ggtgctgaag ttgctttccc ctgcccaaag
     7261 gccgctgcaa atcacgcgtg atttgggggc cttctggaaa ggggcgtatc gtgaggtgca
     7321 aaaggagatg aaagggcgtt atcccaaaca cgtctggccg gacgacccgg caaacacggc
     7381 accgacgcga cggacgaaaa agtattcgta aggttggttt tctccctctc cctgtgggag
     7441 agggccgggg tgagggcatc agcgcgcacg ttcaccctca ccctaaccct ctccctcaag
     7501 ggagagggga ccgatcgagc gcaaattttg agagatatct tcttctgtct tgtaacagaa
     7561 gaacagaaaa tcgggctttt gcgcctgaat attgcggaga aaaagcatgg ccgggaatga
     7621 ccgcgagcca attggacgca aagggaaacc gacgcgtccg gtcaaacaaa aggtaagccg
     7681 tcgtcgttac gaagatgacg atgattacga cgattatgat gactatgagg atgaagaacc
     7741 gatgccgcgc aaaggtaagg gcaaaggcaa agggcgtaag cctcgtggca aacgcggctg
     7801 gctatggcta ctgctaaaac tggctatcgt ttttgccgtg ctgatcgcca tttacggcgt
     7861 ttatctcgat caaaaaattc gtagccgtat tgatggcaag gtctggcaac tgcccgcggc
     7921 agtttatggc cgaatggtca accttgaacc agatatgacc atcagcaaga acgaaatggt
     7981 gaagctgctg gaggcaacac agtatcgtca ggtgtcgaaa atgacccgtc ctggcgaatt
     8041 taccgtgcag gccaacagca ttgagatgat ccgccgtccg tttgatttcc cggacagtaa
     8101 agaagggcag gtgcgtgcgc gtctgacctt tgatggtgat catctggcga ccatcgtcaa
     8161 tatggagaac aaccgtcagt tcggtttctt ccgtcttgat ccgcgtctga tcaccatgat
     8221 ctcctcgcca aacggtgagc agcgtttgtt tgtgccgcgc agtggtttcc ctgatttgct
     8281 ggtggatact ttgctggcga cagaagaccg tcatttttac gagcatgatg gaatcagtct
     8341 ctactccatt gggcgtgcgg tgctggcaaa cctgaccgca ggtcgcacgg tgcagggtgc
     8401 gagtacgctg acgcaacagc tggtgaaaaa cctgttcctc tccagcgagc gttcttactg
     8461 gcgtaaagcg aacgaagctt acatggcgct gatcatggac gcgcgttaca gcaaagaccg
     8521 tattcttgag ctgtatatga acgaggtgta tctcggtcag agcggcgaca acgaaatccg
     8581 cggcttcccg ctggcaagct tgtattactt tggtcgcccg gtagaagagc taagcctcga
     8641 ccagcaggcg ctgttagtcg gtatggtgaa aggggcgtcc atctacaacc cgtggcgtaa
     8701 cccgaaactg gcgctggagc gacgtaatct ggtgctgcgt ctgctgcaac agcaacagat
     8761 tattgatcaa gaactctatg acatgttgag tgcccgtccg ctgggggttc agccgcgtgg
     8821 tggggtgatc tctcctcagc cagcctttat gcagctggtg cgtcaggagt tgcaggcaaa
     8881 actgggcgat aaggtaaaag atctctccgg cgtgaagatc ttcactacct ttgactcggt
     8941 ggcccaggac gcggcagaaa aagccgccgt ggaaggcatt ccggcactga agaaacagcg
     9001 taagttgagc gatcttgaaa ctgcgattgt ggtcgtcgac cgctttagtg gtgaagttcg
     9061 tgcgatggtc ggaggttctg agccgcagtt tgcgggctac aaccgtgcga tgcaggcgcg
     9121 tcgttcgatt ggttcccttg caaaaccagc gacttatctg acggctttaa gccagccgaa
     9181 aatctatcgc ctgaacacgt ggattgccga tgcgccaatc gcgctgcgtc agccgaatgg
     9241 ccaggtctgg tcaccgcaga acgatgaccg tcgttatagc gaaagcggca gagtgatgct
     9301 ggtggatgcg ttgacacgtt cgatgaacgt gccgacggta aatctgggga tggcgctggg
     9361 gctgcctgcg gttacggaga cctggatcaa actgggcgta ccgaaagatc agttgcatcc
     9421 ggttccggca atgctgctgg gggcgttgaa cttaacgcca atcgaagtgg cgcaggcatt
     9481 ccagaccatc gccagcggtg gtaaccgtgc accgctttct gcactgcgtt cggtaatcgc
     9541 ggaagatggt aaggtgctgt atcagagctt cccgcaggca gaacgcgctg ttccggcgca
     9601 ggcggcgtat ctgacactat ggaccatgca gcaggtggta caacgtggta cgggtcgtca
     9661 gcttggggcg aaatacccga acctgcatct ggcagggaaa acagggacta ccaacaataa
     9721 cgtagatacc tggtttgcag gcattgacgg cagcacggtg accatcacct gggtaggccg
     9781 tgataacaac cagccgacca aactgtatgg agccagcggg gcaatgtcga tttatcagcg
     9841 ttatctggct aaccagacgc caacgccgct gaatcttgtt ccgccagaag atattgcaga
     9901 tatgggcgtg gactacgacg gcaactttgt ttgcagcggt ggcatgcgtg tcttgccggt
     9961 ctggaccagc gatccgcaat cgctgtgcca gcagagcgag atgcagcagc agccgtcagg
    10021 caatccgttt gatcagtctt ctcagccgca gcaacagccg caacagcaac ctgctcagca
    10081 agagcagaaa gacagcgacg gtgtagccgg ttggatcaag gatatgtttg gtagtaatta
    10141 acatctaagc gtgaaatacc ggatggcgag ttgccatccg gtaaaataac atcccatcta
    10201 agatattaac cctttctttt catctggttg tttattaacc cttcaggaac gctcagattg
    10261 cgtaccgctt gcgaacccgc cagcgtttcg aatattatct tatctttata ataatcattc
    10321 tcgtttacgt tattattcac tttacatcag agatatacca atggcgcgtt ccaaaactgc
    10381 tcagccaaaa cactcactgc gtaaaatcgc agttgtagta gccacagcgg ttagcggcat
    10441 gtctgtttat gcacaggcag cggttgaacc gaaagaagac actatcaccg ttaccgctgc
    10501 acctgcgccg caagaaagcg catgggggcc tgctgcaact attgcggcgc ggcagtcagc
    10561 taccggcact aaaaccgata cgccgattca aaaagtgcca cagtctattt ctgttgtgac
    10621 cgccgaagag atggcgctgc atcagccgaa gtcggtaaaa gaagcgctta gctacacgcc
    10681 gggtgtctct gttggtacgc gtggcgcatc caacacctat gaccacctga tcattcgcgg
    10741 ttttgcggca gaaggccaaa gccaaaataa ctatctgaat ggcctgaagt tgcagggcaa
    10801 cttctataac gatgcggtca ttgacccgta tatgctggaa cgcgctgaaa ttatgcgtgg
    10861 cccggtttcc gtgctttacg gtaaaagcag tcctggcggt ctgttgaata tggtcagcaa
    10921 gcgtccgacc accgaaccgc tgaaagaagt tcagtttaaa gccggtactg acagcctgtt
    10981 ccagactggt tttgacttta gcgatgcgtt ggatgatgac ggtgtttact cttatcgcct
    11041 gaccggtctt gcgcgttctg ccaatgccca gcagaaaggg tcagaagagc agcgttatgc
    11101 tattgcaccg gcgttcacct ggcgtccgga tgataaaacc aatttcacct tcctttctta
    11161 cttccagaac gagccggaaa ccggttatta cggctggttg ccgaaagagg gaaccgttga
    11221 gccgctgccg aacggtaagc gtctgccgac agactttaat gaaggggcga agaacaacac
    11281 ctattctcgt aatgagaaga tggtcggcta cagcttcgat cacgaattta acgacacctt
    11341 tactgtgcgt cagaacctgc gctttgctga aaacaaaacc tcgcaaaaca gcgtttatgg
    11401 ttacggcgtc tgctccgatc cggcgaatgc ttacagcaaa cagtgtgcgg cattagcgcc
    11461 agcggataaa ggccattatc tggcacgtaa atacgtcgtt gatgatgaga agctgcaaaa
    11521 cttctccgtt gatacccagt tgcagagcaa gtttgccact ggcgatatcg accacaccct
    11581 gctgaccggt gtcgacttta tgcgtatgcg taatgacatc aacgcctggt ttggttacga
    11641 cgactctgtg ccactgttca atctgtacaa tccggtgaat cccgatttcg acttcaatgc
    11701 caaagatccg gcaaactccg gcccttaccg cattctgaat aaacagaaac aaacgggcgt
    11761 ttatgttcag gatcaggcgc agtgggataa agtgctggtc accctaggcg gtcgttatga
    11821 ctgggcagat caagaatctc ttaaccgcgt tgctgggacg accaataaac gtgatgacaa
    11881 acagtttacc tggcgtggtg gtgttaacta cctgtttgat aatggtgtaa caccttactt
    11941 cagctatagc gaatcgtttg aaccttcttc gcaagttggg aaggatggta atattttcgc
    12001 accgtctaaa ggtaagcagt atgaagtcgg cgtgaaatat gtaccggaag atcgtccgat
    12061 tgtagttact ggtgccgtgt ataatctcac taaaaccaac aacctgatgg cggaccctga
    12121 gggttccttc ttctcggttg aaggtggcga gatccgcgca cgtggcgtag aaatcgaagc
    12181 gaaagcggcg ctgtcggcga gtgttaacgt agtcggttct tatacttaca ccgatgcgga
    12241 atacaccacc gatactacct ataaaggcaa tacgcctgca caggtgccaa aacacatggc
    12301 ttcgctgtgg gctgactata ccttctttga cggtccgctt tcaggtctga cgctgggcac
    12361 cggtggtcgt tatactggct ccagctatgg tgatccggct aactccttta aagtgggaag
    12421 ttatacggtc gtggatgcgt tagtgcgtta tgatctggcg cgagtcggca tggcgggctc
    12481 caacgtggcg ctgcatgtta acaacctgtt cgatcgtgaa tacgtcgcca gctgctttaa
    12541 cacttatggc tgcttctggg gcgcagaacg tcaggtcgtt gcaaccgcaa cctttcgttt
    12601 ctaatttctc ttttggggca cggatttccg tgcccatttc acaagttggc tgttatgcag
    12661 gaatacacga atcattccga taccactttt gcactgcgta atatctcctt tcgtgtgccc
    12721 gggcgcacgc ttttgcatcc gctgtcgtta acctttcctg ccgggaaagt gaccggtctg
    12781 attggtcaca acggttctgg taaatccact ctgctcaaaa tgcttggtcg tcatcagcct
    12841 ccgtcagaag gggagattct tcttgatgcc cagccgctgg aaagctggag cagcaaagcg
    12901 tttgcccgca aagtggctta tttgccgcag cagcttcctc cggcagaagg gatgaccgtg
    12961 cgtgaactgg tggcgattgg tcgttacccg tggcatggcg cgctggggcg ctttggtgcg
    13021 gcagatcgcg aaaaggtcga ggaagctatc tcgctggttg gcttaaaacc gctggcgcat
    13081 cggctggtcg atagtctctc tggcggcgaa cgtcagcggg cgtggatcgc catgctggtg
    13141 gcgcaggata gccgttgtct gttgctcgac gaaccgactt cagcgctgga tatcgcccac
    13201 caggttgatg tgctggcgct ggtgcaccgt ttaagtcagg aacgtggcct gacggtcatt
    13261 gccgtgttgc acgatatcaa tatggcggca cgctactgtg attatctggt cgccctgcgc
    13321 agtggtgaaa tgattgctca gggaacgcct gcggaaatta tgcgcggcga aaccctcgaa
    13381 atgatttatg gcatcccgat gggtattttg ccgcatccgg cgggtgctgc acctgtgagt
    13441 tttgtttatt gatgagcggc ttacctctta tttcgcgccg tcgactgtta acggcgatgg
    13501 cgctttctcc gttgttatgg cagatgaata ccgcccacgc agcggctatt gaccccaatc
    13561 gtattgtggc gctggagtgg ttgccggtgg aattactgct ggcgctcggc atcgtgcctt
    13621 acggcgtggc ggataccatc aactatcgcc tgtgggtcag cgaaccacca ttgccggact
    13681 cagtgattga cgtcggtttg cgtacagaac ctaaccttga actgctgacc gaaatgaaac
    13741 cgtcgtttat ggtctggtcg gcaggatatg gcccttcatc agaaatgctg gcacgtattg
    13801 cgccgggtcg cggatttaac ttcagtgacg gcaaacatcc gttggcgatg gcgcgtaaat
    13861 cgctgacgga aatggcagat ttacttaacc tgcaaagcgc agcggaaacg catttagcgc
    13921 aatacgaaga ctttatccgc agcatgaaac cccgctttgt gaagcgtggt gcacgcccgt
    13981 tactgctgac gacgcttatc gatccgcgcc atatgctggt cttcggtcca aacagcttgt
    14041 tccaggaaat tctcgatgag tacggcatcc caaatgcctg gcaaggggaa accaacttct
    14101 ggggcagtac cgccgtcagt atcgatcgtc tggcagcgta taaagacgtt gatgtgctct
    14161 gttttgatca cgacaacagc aaagacatgg atgcgctaat ggcaaccccg ctgtggcagg
    14221 ccatgccgtt tgtccgtaca gggcgctttc agcgcgtacc cgcagtctgg ttttatggtg
    14281 caacgctttc ggcaatgcac tttgtgcgtg ttctggataa cgccatcgga ggtaaagcgt
    14341 gagtaaacga attgcgcttt tcccggcgtt attgctggcg ctgttagtga ttgtcgctac
    14401 ggcgctcacc tggatgaact tctcacaggc gctgccgcgc agtcagtggg cgcaggccgc
    14461 ctggtcgccg gatattgacg tcatcgagca gatgattttt cactacagct tgttgccgcg
    14521 tctggcgatt tcgctgctgg tgggcgcggg cctggggctg gtgggcgtgc tgtttcagca
    14581 agtactgcgt aacccgctgg cggaaccgac gacgttgggc gttgcaacag gcgcgcaact
    14641 ggggattacc gtcaccacgc tctgggcgat ccctagcgcg atggcgagcc agtttgctgc
    14701 gctggcaggg gcttgcgttg ttggcttaat tgtctttggc gtcgcgtggg ggaaacggct
    14761 gtcgccggta acgctgattt tcgcggggtt ggtagtgagc ctttattgcg gcgcaatcaa
    14821 tcagttactg gttatcttcc atcatgacca gttgcaaagc atgtttctgt ggagcactgg
    14881 aacgctgacg caaaccgact ggggcggtgt tgagcgttta tggccgcagt tgctgggcgg
    14941 tgtgatgctg acgttgctgc tacttcgtcc gttaaccctg atggggcttg atgatggcgt
    15001 ggcgcgcaat ctcgggctgg ccttgtcgct tgcgcgtctg gcggcgctgt cgctggcgat
    15061 tgtcatcagt gcgctgctgg tgaacgctgt ggggattatc ggctttatcg ggttgttcgc
    15121 gccgctgctg gcaaaaatgc tgggggcgcg gcgtctgctg ccacgactga tgctggcgtc
    15181 gttgattggt gcgctgatcc tctggctttc cgatcaaatc atcctctggc tgactcgcgt
    15241 gtggatggaa gtgtccaccg gttcggtcac tgcgttgatc ggtgcgccgc tgctactgtg
    15301 gctgctgccg cgtttacgca gcattagcgc gccggatatg aaggttaacg atcgtgtcgc
    15361 gactgaacgc caacatgtgc tggcgtttgc cctcgcgggc ggcgtgctgt tgttgatggc
    15421 tgtggtggtg gcgctgtcgt ttggtcgtga tgcgcacggc tggacgtggg cgagcggggc
    15481 gttgctcgag gatttaatgc cctggcgctg gccgcgaatt atggcggcgc tgtttgcggg
    15541 cgtcatgctg gcggtagcgg gctgtattat tcagcgactg accggcaacc cgatggcaag
    15601 cccggaagtg ctggggatta gctccggcgc ggcgtttggt gtggtgttaa tgctgtttct
    15661 ggtaccgggt aatgcctttg gctggttgtt acccgcaggc agtctcgggg cggcggtgac
    15721 gctgttgatc attatgatcg ccgccggacg cggtggattt tccccacacc gcatgttact
    15781 ggcggggatg gcgttaagta ccgcgttcac catgcttttg atgatgttgc aggcaagtgg
    15841 tgacccgcga atggcgcaag tgctgacctg gatttccggt tcgacctaca acgcgaccga
    15901 tgctcaggtc tggcgcaccg gaattgtgat ggtgattttg ctggcgatta ctccgctgtg
    15961 ccgccgctgg ctgaccattt taccgctggg tggtgatacc gcgcgggcgg tagggatggc
    16021 gctgacgccg acgcgaattg cgctgctgct gttagcggct tgcctgacgg cgaccgcgac
    16081 gatgactatt ggaccgttga gttttgttgg tttaatggca ccgcatattg cgcggatgat
    16141 gggctttcga cggacgatgc cacacatcgt aatttcggcg ctggtgggtg gtttactgct
    16201 ggtgttcgct gactggtgtg ggcggatggt gctgtttcca ttccagatcc cggcggggct
    16261 gctgtcgacc tttatcggtg cgccatattt tatctatttg ctgagaaagc agagccgtta
    16321 atagaatgcc tcgcccggtg ggcgaggcaa aattatcaca acttcgcgaa cacccgacgt
    16381 gcagcatcga tggtgttatt gatatcttcc atgctgtgcg ccacagacat aaagcctgct
    16441 tcaaacgctg acggtgccag gtaaacacct tcatccagca tcatatggaa gaaacgctta
    16501 aaacgctcga cgtcacaggc catcacatcc tgatagcacg tcacggactc ggcgtcggta
    16561 aagaagatac cgaacatacc accgacgtgg ttaacgacca gcggaattcc cgcttcttct
    16621 gccgcttcca gcagaccttc tgccagacgt gttgtcagct catccagcgt ttcgtgaatg
    16681 cccggctgcg cgacttcatt cagacaggcg aaacccgctg ccatcgcaat cgggttaccg
    16741 gaaagcgtac ccgcctgata gaccggacct gtcggggcca gcgcatccat tacatcacga
    16801 cgaccaccga atgcgcctac cggcattcca ccgccgatga tttttccgag gcaggttaaa
    16861 tctggcacta cggcgaaata atcctgtgcg ccagctagcg ctacgcggaa accggtcatc
    16921 acttcatcga tgatcagcaa cgcgccaaat tcgtcgcaca gcgcacgcag acctggcagg
    16981 aactctggca gcggcggaac acagttcata ttgcctgcca ccggctcgac gataatacag
    17041 gcaatctctt gcgggtattg ctcaaacgcg gcgcgtacag aggccagatc gttataagta
    17101 caggttaagg tatgtttggc gaaatctgcc ggaacgcccg gcgagtttgg ctggcctaac
    17161 gtgagtgcgc cagaaccggc tttcaccagc aggcagtcag cgtgaccgtg gtaacaacct
    17221 tcaaatttaa tgattttgtc gcgaccggta aaaccacggg ccaggcggat ggcgctcatc
    17281 gtcgcctcgg tgccggagtt caccatgcgc accatatcca tggtcgggac cagttcggtc
    17341 accagttgcg ccattttcac ttccatttcg gtcggtgcac caaagcttaa accacgctcg
    17401 gcggcttcaa tcacggcatt gcggatcgcc ggatggttat ggcccagcac catcggcccc
    17461 caggaaccga cataatcgat ataggctttg ccatcaacat cgtacagata agcgccgtcc
    17521 gctttttcga taaacagtgg agtgccgccc acgccagtaa aggcgcgaac aggggagttc
    17581 acaccgccag ggatcagctc gcgcgctgcg ctgtaaagat tttcagactt actcatggag
    17641 ggttcctgat tcgtagaaaa agtgaatggc tgctattcca tgttattcat aacaagttaa
    17701 atactcgtca aacatcaggc tgcttgtact ggtgcaatcc tgatttctta gagtataaaa
    17761 gttttgtgca tttgaaacat tacgctttgc aaaggatttt catggaacgt gcgagtaaaa
    17821 tgccgtcatc ttatttgtat gaccaataag tgatcattgg atgaaaactg atactccctc
    17881 tttagaaaca ccgcaggccg cgcgcctgcg acgcagacaa ctgattcgcc aacttcttga
    17941 gcgcgataaa accccgttag ccattttgtt tatggcggca gtcgtcggca cgcttgtcgg
    18001 gctggctgcg gttgcttttg acaaaggtgt cgcctggttg cagaaccaac gtatgggggc
    18061 gctggtacat actgctgata attatccgct actgttaacc gtcgcttttc tctgttcggc
    18121 ggtgctggcg atgttcggct actttttggt gcgcaaatac gcgcccgaag cagggggatc
    18181 ggggatcccg gaaattgaag gggcgctgga agatcaacgt cctgttcgct ggtggcgtgt
    18241 attgccggtg aaattctttg gcgggctggg gacactcggc ggaggcatgg ggctagggcg
    18301 cgaaggacca accgtgcaga tcggcggcaa tatcagtcgc atggtgcttg atattttccg
    18361 cctgaaaggt gatgaagctc gccatacgct gctggcaacc ggtgctgctg cggggctggc
    18421 tgcggccttt aacgcgccgc tggcgggtat tttgtttatt atcgaagaga tgcgtccgca
    18481 gtttcgctat acgttaattt cgattaaagc ggtatttatt ggcgtcatta tgtcgaccat
    18541 tatgtaccgg atttttaatc atgaagttgc gttgattgac gtcggtaaac tttctgatgc
    18601 gccgcttaat acgctgtggc tttatctgat cctcggtatt atttttggca ttttcggccc
    18661 tatttttaat aaatgggtgc tggggatgca ggatttgctg caccgtgtgc acggcggcaa
    18721 tattaccaaa tgggtactga tggggggggc gattggcggt ctgtgtggat tgctggggtt
    18781 tgtggcacca gcaacgtcgg gcggcggttt taacctgatt cctatcgcta ccgcggggaa
    18841 tttcagcatg ggaatgctgg tgtttatctt cgtcgctcgg gtcattacca ccttactctg
    18901 cttctcttcc ggcgcgccgg gcggtatttt tgccccgatg ctggcgctgg gtactgtgct
    18961 gggaaccgct ttcggaatgg ttgccgttga gctgtttccg caatatcacc tggaggcggg
    19021 gacgtttgct attgccggaa tgggggcatt actggcggca tctattcgcg cgccgttaac
    19081 ggggatcatt ctggttctgg agatgaccga taactaccag ctcattttgc caatgattat
    19141 taccggtctt ggcgcaacac tattagcgca atttaccggc gggaaacctc tatactcggc
    19201 gattcttgcg cgcacgctgg caaaacagga agctgagcaa ctggcgcgaa gcaaggccgc
    19261 atcagccagc gagaatactt gaacgaaata ccagggtatt agataatggc gattattatt
    19321 gggttagaat ttgcccgatt gccgatgtcg tttggagcaa aatatgagtg atgacgtagc
    19381 actgccgctg gagtttaccg acgcagcagc caacaaagtt aaaagcctga tcgctgacga
    19441 agataacccg aatctgaaat tacgcgtgta tatcaccggt ggcggttgca gcggcttcca
    19501 gtatggtttc acctttgatg atcaggtgaa cgaaggcgat atgaccatcg aaaaacaggg
    19561 cgttggcctg gtggttgatc cgatgagcct gcaatatctg gtcggcggtt ccgttgacta
    19621 caccgaagga ctggaaggtt ctcgtttcat cgtgaccaac ccgaacgcga aaagcacctg
    19681 cggttgcggt tcttccttta gcatctaatc tgttgttcat gtgccggata aactatccgg
    19741 cacatcttct ctcttcaacg cccattctca tccagcgcaa acgtcggtag cttaagatgc
    19801 caacgaatag ccgccagccg aatcaatagc gtcacgacca tgcccatcat actggctgtt
    19861 tccagtggta cggaaaatgt gtaataagcc gtagcgtgga caataccgcc gataatacag
    19921 gcagttgcgt agatttctgt acgtaaaatc atggggattt cgcggaccag tacatcacga
    19981 atgatcccgc cgccaacgcc agtaatgacg cccatacaaa ccgcgattaa cggaccggct
    20041 tccgcattaa aggctttatt cacgctaatg ccgacaaaca ccgccagacc aacggcgtcc
    20101 agcaccggca acatccattt tggtaagcgt cttggctggc gcaccagcac gatggtcagc
    20161 atgctggtga ccatggcaac gaccagatcg gtgggatctt tcacccaaaa taccgggccg
    20221 tgatccagcg ccatgtcgcg aattgttccg tcgcctactg cggtaaccac gcccagtacc
    20281 agaacaccaa aagggtccat acgcaatttt ccggctaaca aaacgccgta gatggcaaat
    20341 accgctgtgc cgactatatc cagccaatag acgagcattg ttcaatcccc actgagcacc
    20401 tgaaaaggtc aggcactaat ctacctgtga aagcgcatta cagagctgtt gtgctgcgag
    20461 gataatacgt gggcttgcac gttcaaacca gtcactcgtg agaggaataa cgggaatttt
    20521 gagctgttca ccccagtatt gtttgatttt aggaatttgg tccggtccgc ctgtaatgac
    20581 aatagcctgt ggcgagcgtg ctaacacctg ttcgcggcta acttgcggcc agggaacccg
    20641 gctgtcttta aagatgtttt ctccgccaca aacttcgagt accaggttct gaatcgactc
    20701 ttttccactg gtaaatggcg gattaatgcc gaattgcaga aaaacacgtt ttttaggttt
    20761 atcagcatat tgcgctttca attgcacgta ctgatccagc agggattgcg cggcttgttc
    20821 ggccttgtct ggttgcggac tccagggggc cagttgacgt aacgcattgg caatttgttc
    20881 aatgcttgtc gcatcgaccc acatcacttt tattcccagc gaagccagct ggtcaacctg
    20941 ccgctcggca ttacctccac gccaggcaat caccagatcg ggtttcagcg cgacaatgcg
    21001 ttccagattc atcccctgcc aggtggaaac ctgctcaatc ttttgcgctt gtggaggata
    21061 gtcggaatag ctgctgaccc caaccggcgt gatcccggcg gcaaaggcaa gttcagtgtt
    21121 ggcgggagaa agcgtgatga cgcgcggcgc ggcgttgagc cacagtggcg cgagaaaaga
    21181 cagggcgacc agcgccctga acagtgattt agccatgtgc cagtttctgc accagtgact
    21241 caaccatcag gctggactgt ttagcggcaa cagccaggaa ctcatcgaag ctaagatgag
    21301 actgttgatc ggccacgtcg gagatggcgc gtacgacaac aaacgggacg ttgaaattgt
    21361 ggcagacatg ggcgattgcc gtcgcttcca tctctacagc aatggcctgt gggaagttgt
    21421 ggcggatttt cgccagacca acagaaccgt tgatgaaagc gtcgccgcta acaatcaggc
    21481 cacgtacagc gttaagattc agttcggcaa tgcaggcctc agcggcagcg atcagtttat
    21541 cgtcagcttt aaagcctgcc ggacagcctg gtaactgacc gtattcataa ccaaatgccg
    21601 tgacatccgc gtcgtgataa cgcgcttcgt ccgagacaac gatatcgccc actttcagcg
    21661 ttggtgccag gccaccggca gaaccggtgt taataatcac atctggcttg cagtgttcca
    21721 acagcagagt ggcacccagc gccgcagcga ctttaccgat gcccgatttc agaagcgcaa
    21781 cctcggttcc attcagttgg ccggtataga tttcgcaacc gccgagactg atagtttgac
    21841 ggttttcgat tttgtcacgc agcagcgtaa cttcttcttc cattgcacca atgatgccga
    21901 ttttcataga tttactcgcg ataagcccga tttgaaggca tagtttacca tgcgcttacg
    21961 gggaagcgta tttctcacgc gggagaggac atggcacaga ttgatttccg aaaaaaaata
    22021 aactggcatc gtcgttaccg ttcaccgcag ggcgttaaaa ccgaacatga gatcctgcgg
    22081 atcttcgaga gcgatcgcgg gcgtatcatc aactctccgg caattcgtcg tctgcaacaa
    22141 aagacccagg tttttccact ggagcgcaat gccgccgtgc gcacgcgtct tacccactcg
    22201 atggaagtcc agcaggtggg acgctacatc gccaaagaaa ttttaagccg tctgaaagag
    22261 cttaaattgc tggaagcata cggcctggat gaactgaccg gcccctttga aagcattgtt
    22321 gagatgtcat gcctgatgca cgatatcggc aatccgccgt ttggtcattt tggcgaagca
    22381 gcgataaatg actggtttcg ccaacgtttg cacccggaag atgccgaaag ccagcctctg
    22441 actgacgatc gctgcagcgt ggcggcacta cgtttacggg acggggaaga accgcttaac
    22501 gagctgcggc gcaagattcg tcaggactta tgtcattttg aggggaatgc acaaggcatt
    22561 cgtctggtgc atacattgat gcggatgaat ctcacttggg cacaggttgg cggtatttta
    22621 aaatataccc gtccggcgtg gtggcgtggc gaaacgcctg agacacatca ctatttaatg
    22681 aaaaagccgg gttattatct ttctgaagaa gcctatattg cccgcttgcg taaagaactt
    22741 aatttggcgc tttacagtcg ttttccatta acgtggatta tggaagctgc cgacgacatc
    22801 tcctattgtg tggcagacct tgaagatgcg gtagagaaaa gaatatttac cgttgagcag
    22861 ctttatcatc atttgcacga agcgtggggc cagcatgaga aaggttcgct cttttcgctg
    22921 gtggttgaaa atgcctggga aaaatcacgc tcaaatagtt taagccgcag tacggaagat
    22981 cagtttttta tgtatttacg ggtaaacacc ctaaataaac tggtacccta cgcggcacaa
    23041 cgatttattg ataatctgcc tgcgattttc gccggaacgt ttaatcatgc attattggaa
    23101 gatgccagcg aatgcagcga tcttcttaag ctatataaaa atgtcgctgt aaaacatgtg
    23161 tttagccatc cggatgtcga gcagcttgaa ttgcagggct atcgggtcat tagcggatta
    23221 ttagagattt atcgtccttt attaagcctg tcgttatcag actttactga actggtagaa
    23281 aaagaacggg tgaaacgttt ccctattgag tcgcgcttat tccacaaact ctcgacgcgc
    23341 catcggctgg cctatgtcga ggctgtcagt aaattaccgt cagattctcc tgagtttccg
    23401 ctatgggaat attattaccg ttgccgcctg ctgcaggatt atatcagcgg tatgaccgac
    23461 ctctatgcgt gggatgaata ccgacgtctg atggccgtag aacaataacc aggcttttgt
    23521 aaagacgaac aataaatttt taccttttgc agaaacttta gttcggaact tcgggctata
    23581 aaacgaatct gaagaacaca gcaattttgc gttatctgtt aatcgagact gaaatacatg
    23641 aaaaaaaaca cattagcact gagtgcactg gctctgagtt taggtttggc gttatctccg
    23701 ctctctgcaa cggcggctga gacttcttca gcaacgacag cccagcagat gccaagcctt
    23761 gcaccgatgc tcgaaaaggt gatgccttca gtggtcagca ttaacgtaga aggtagcaca
    23821 accgttaata cgccgcgtat gccgcgtaat ttccagcagt tcttcggtga tgattctccg
    23881 ttctgccagg aaggttctcc gttccagagc tctccgttct gccagggggg ccagggcggt
    23941 aatggcggcg gccagcaaca gaaattcatg gcgctgggtt ccggcgtcat cattgatgcc
    24001 gataaaggct atgtcgtcac caacaaccac gttgttgata acgcgacggt gattaaagtc
    24061 cagctgagcg atggtcgtaa gttcgacgcg aagatggttg gcaaagatcc gcgctctgat
    24121 atcgcgctga tccaaatcca gaacccgaaa aaactgaccg caattaagat ggcggattct
    24181 gatgcactgc gcgtgggtga ttacaccgta gcgattggta acccgtttgg tctgggcgag
    24241 acggtaactt ccgggattgt ctctgcgctg gggcgtagcg gcctgaatgc tgaaaactac
    24301 gaaaacttca tccagaccga tgcagcgatc aaccgtggta actccggtgg tgcgctggtt
    24361 aacctgaacg gcgaactgat cggtatcaac accgcgatcc tcgcaccgga cggcggcaac
    24421 atcggtatcg gttttgctat cccgagtaac atggtgaaaa acctgacctc gcagatggtg
    24481 gaatacggcc aggtgaaacg cggtgagctg ggtattatgg ggactgagct gaattccgaa
    24541 ctggcgaaag cgatgaaagt tgacgcccag cgcggtgctt tcgtaagcca ggttctgcct
    24601 aattcctccg ctgcaaaagc gggcattaaa gcgggtgatg tgatcacctc actgaacggt
    24661 aagccgatca gcagctttgc cgcactgcgt gctcaggtgg gtactatgcc ggtgggcagc
    24721 aaactgaccc tgggcttact gcgcgacggt aagcaggtta acgtgaacct ggaactgcag
    24781 cagagcagcc agaatcaggt tgattccagc tccatcttca acggcattga aggcgctgag
    24841 atgagcaaca aaggcaaaga tcagggcgtg gtagtgaaca acgtgaagac gggcactccg
    24901 gctgcgcaga tcggcctgaa gaaaggtgat gtgattattg gtgcgaacca gcaggcagtg
    24961 aaaaacatcg ctgaactgcg taaagttctc gacagcaaac cgtctgtgct ggcactgaac
    25021 attcagcgcg gcgacagcac catctacctg ttaatgcagt aatctccctc aaccccttcc
    25081 tgaatacggg aaggggttct ccttacaatc tgtgaacttc gccacaactc catacatctt
    25141 catcatcctt tgggcatttg cacaatgccg tacgttacgt acttccttat gctaagccgt
    25201 gcataacgga ggacttatgg ctggctggca tcttgatacc aaaatggcgc aggatatcgt
    25261 ggcacgtacc atgcgcatca tcgataccaa tatcaacgta atggatgccc gcgggcgaat
    25321 tatcggcagc ggcgatcgtg agcgtattgg tgaattgcac gaaggtgcat tgctggtact
    25381 ttcacaggga cgagtcgtcg atatcgatga cgcggtagca cgtcatctgc acggtgtgcg
    25441 gcaggggatt aatctaccgt tacggctgga aggtgaaatt gtcggcgtaa ttggcctgac
    25501 aggtgaacca gagaatctgc gtaaatatgg cgaactggtc tgcatgacgg ctgaaatgat
    25561 gctggaacag tcgcggttga tgcacttgtt ggcgcaggat agccgtttgc gggaagaact
    25621 ggtgatgaac ctgattcagg cagaggagaa tactcccgca cttctgaatg ggcgcaacgg
    25681 ctggggatcg atctcaatca accgcgagtg gtggctattg ttgaggtcga cagcggtcag
    25741 cttggcgtgg acagcgcaat ggcggagtta caacaactgc aaaacgcgct gactacgccc
    25801 gagcgtaata atctggtggc gattgtctcg ctaaccgaaa tggtggtgtt gaaaccggcg
    25861 ttgaactctt ttgggcgctg ggatgcagaa gatcatcgta agcgagttga acaactgatt
    25921 acccgcatga aagagtacgg ccagctgcgt tttcgcgttt cactgggcaa ctattttacc
    25981 ggtcctggca gtattgcccg atcctatcgt acggcgaaaa cgacgatggt ggtgggtaaa
    26041 cagcggatgc cagaaagtcg ctgctatttt tatcaggatc tgatgttacc tgtgttactc
    26101 gacagtttgc gtggcgactg gcaggccaac gaactggcgc gaccgctggc gcggctgaaa
    26161 gcgatggaca ataacggctt gctgcgacga acgctggcgg cgtggtttcg ccacaatgtg
    26221 caaccgctgg caacgtcaaa ggcgttgttt attcatcgta ataccctgga gtatcggctt
    26281 aatcgtatat cggaactgac cgggcttgat ttgggcaatt ttgatgacag gttgctgctg
    26341 tatgtggcgt tacaactgga tgaagagcgg tagattatgc gttaaggttg aggcggcgct
    26401 ggctcattcc cctcagcccg aaggggagag gggaatgccc gagttaaccg tcaattcggg
    26461 attatttacg cgttagtttt tccagatcgg cttcaatctc gctgatctta ttggttacga
    26521 ctgactccag gtgacgtaag tcgtcgagga tcttacgctt aagatcaact tcgctgcggt
    26581 cacgctgaca gatttgatca agctcatcaa tgatataccg tagattcggg ctgatttcct
    26641 ggacttcttt ataaccctga cccacaccat cagcgacgac cgttttacgc tgacgcggat
    26701 atttaaactt aacgctcttg gcgaaaaact cgcctttgtc tttctggaaa tagattttca
    26761 gaatatcgtt gttggcttcc tgccggaggc tgtaacgatc aatttcttca ggattggtaa
    26821 tacccagact tttcagattg tcgtacatag cgttacctca aaatgagtca gtaaattgtg
    26881 cttattttag catttggctt ggcccgcccg gctcgactgt gatcgcagaa agcctttttc
    26941 ttccagactt ctatcatggc gtaaaaatca aaaaattacc tgctttattg tggcgatatt
    27001 ccatgggatg gattagcaca tttatcttcg ttttgcgaat tacatgacag tccgaaacaa
    27061 gatgaaaaag tatttaaaaa agggggagga agaatgagta ggacgaatta ttctgagcca
    27121 gaaagcatac gggcagcatc atgctgcccg tatatgtact cttaatcgat ggtacgcagc
    27181 agttcgttaa tgccgacttt gccgcgagtt ttcgcgtcaa ctttcttaac gataaccgca
    27241 cagtagaggc tgtatttgcc atcttttgac ggcagattac ctgaaacaac cacagacccc
    27301 gccggaacgc gaccgtagtg gatttcgccg gtttcacggt cgtaaatacg ggtgctctga
    27361 ccaatgtata cgcccataga gatgacggaa ccttcttcga caatcacccc ttcaaccact
    27421 tcagagcgtg cgccaatgaa gcagttatct tcaataatgg tcggggtagc ctgcagcggc
    27481 tccagcacgc cgccgatgcc cacgccaccg gaaaggtgga cgtttttacc aatctgcgca
    27541 caagaaccga cggtcgccca ggtatcaacc atggtgcctt catcaacata tgcgccgatg
    27601 ttaacgtaag acggcatcag cacggtgtta cgggcaataa acgcaccctg acgtaccgcc
    27661 gctggtggca caacgcggaa gccttctttc tggaaacgtg cttcgtcgta gtcggcgaat
    27721 ttcatcggca ctttgtcgaa gtagcggctt tctgcccctt cgatcacctg attatcatta
    27781 atacggaaag agagcagcac cgcttttttc aaccactgat gcgtcaccca ctgaccgtca
    27841 attttttccg ctacacgcag tgcgccggaa tccagcaggg cgatcacctg atttaccgct
    27901 tcgcgggtaa cggtgtctgc attggctggc gtgatctcgg cacggcgttc aaaagcggtt
    27961 tcaataatgt tctgtaactg ctgcattgtt aaactctttt catatcagta aacacatcac
    28021 cctttatcgt ttggattgag ggcctctgtc aaccgctgat gcacttcctg ctgcaactcg
    28081 ttattaagcg cacgccggtc agcggtggca attatgaata aatcttctac tcgctcgcca
    28141 atggttgtaa ttcgggcacc atgaagcgaa attcccagat cggcaaaaat tttcccgact
    28201 cgcgccagca gtccaggttg gtcgagggcg atcagttcga ggaacgattt gcggtcggta
    28261 tgggtcggca aaaacgttac ttcggtttca acagtaaaat ggcgtaattt ggcgggttgg
    28321 cgacggggct gcggtggctg ccagctactt tgcgtcagca cctgctccag accaaaccga
    28381 ataacctcat ggcgatctgc ggacagcggg ctgccatcgg gttccagcac gataaaggta
    28441 tccatcgcca taccgtcgcg agtggtgaaa atttgtgcgt cgtgaacact taaattgcgg
    28501 cggtctaatt cggcacagac ggcggcaaac agataggggc ggtccgggct ccagataaaa
    28561 atctcggtgc ctccacgcgt agcctgcggg ctaagcaata ccagcggttt gcttaaatca
    28621 tgctgtaata aatggcgggc atgccaggcc agttgatttg ggctatggcg gacaaaatag
    28681 ttagcacgac agcgtgacca aatttggtgc agcgcctctt cgtcgatgtt atccatgcgc
    28741 agtagtgcca gtgcctggag ttggtgatgg cgaacccgtt cgcgcatatc cggcgtgttt
    28801 tgcattccgc gtcgtagctg cttttcggtg gcaaaataga gttcgcgcaa cagactttgc
    28861 ttccagctat tccacagtgt ttcgttggtg gcgcaaatgt cagccactgt caggcatacc
    28921 agatagcgca gacgattttc cgtttgaact tcttcggcaa actgcttgat gacttcaggg
    28981 tcctgaatat cgcggcgttg tgctgtcacc gacatcagca agtgctggcg aaccagccag
    29041 gcaaccagct gtgtttcgcg tgagttcagc ccgtggagtt ctgcaaaatg cactacatcc
    29101 tgagcaccga gaatggagtg gtcgccgccg cgtcctttgg cgatatcgtg aaacagcgcg
    29161 gcgatgaaaa tcagctcagt tgacggcagg cgcggccaga cgtccacaca caacggatgg
    29221 cgctggcgcg tttcttcact ggcaaaactc tccagtttca gcatcacgcg gatagtatgt
    29281 tcatccaccg tgtaggcgtg gaacagatca aactgcatct gcccgacgat atgcgaccat
    29341 tgcggcatat acgcgccgag cacgctatgg cgatgcattg gcaatagtcc gcgccgcacc
    29401 gctccggggt gacgcagaat gctcaaaaac agttttcgtg cttgcggaat attacacagc
    29461 ggttgttgca gatggcgacg ggcatggcgt aactggcgca gcgtggtgga gtaaatgccg
    29521 gtgatcgcac tgttgcgcac catggtgtag aacatacgca agatggcttc cggctggcgc
    29581 ataaatagtg tttcatcacg caggtcgatt agcgtaccgc gtagctgaaa ctcatcgtcg
    29641 agtggacgtg gtttttcgtc ggcgggaagg gcgaggatgg cttcatcgaa cagttgcagc
    29701 agcatctggt tgagttcact gacgcggcgt gtaacgcgga agtaatcctt catcatccgc
    29761 tcgaccggtt cgttgccttc accactgtaa ttcagacgct gagcgacgct aagttggcga
    29821 tcgaataaca gacgattatc gtaacggctg acgaccagat gcagggcaaa gcgaatacgc
    29881 cacagtatat gcagacattc gtttaattcc gcccgctccg ctgaggttaa gaagccaaac
    29941 ccgaccattt catccagcga tgttgcgcca aaatgacggc gggccaccca ttgcagagtg
    30001 tgaatatcgc gcaagccgcc agggctactt ttgatgtctg gttcaaggtt gtagctggtg
    30061 ccatggtaac gctgatggcg ctggttctgt tcttcaactt tcgctgcgta gaacttgtcg
    30121 gaaggccaga atccttcgct aaaaatatgt ttttgcagtt cgaggaagag cgcaacatcg
    30181 ccaattaata agcgggattc gattaaattg gtggcgacgg ttaaatccga taacccttcc
    30241 aacatgcact cttcaagcgt gcgcacacta tgaccgactt ccagctttac atcccagagc
    30301 agcgttaaca gctcgcccac tttttgcgcc tgatcgtccg ggagcttttt acggcttaaa
    30361 atcagtaaat cgatgtcaga aagtggatgc agctcgccac gaccgtagcc accgacggca
    30421 accaatgcca ggtcggcaat ctggctgaat cccgcttcaa tccataatcg ttgcaggagc
    30481 tggtctatga actcggtacg tgcctcaatc aactgttctg cagagatccc attgtcaaag
    30541 gcatcaccca gccaacgctg gaaagtatcg atatgggctt ttatcccaca gacggttaat
    30601 tcatcacggg gccaggcgca tggattttgc ggttgaccgg gcagggtgga gagagcggtg
    30661 tttgcgtact gttctggaag ggtattcatt gtgcgccacc cataagatta aattatcgca
    30721 ttaaaaaagc cggcattcgc cggcttcatc ttattcgtcg tgcgagatta tcgccgggat
    30781 ggtgtcatcc ttgcgtagcg tcaaaatttc gcagccgtta tcagtcacca caatagtatg
    30841 ctcatattgt gcagacaagc tgcgatcttt ggtttttacc gtccagccat ctttcatggt
    30901 gcggatctct tttttacccg cgttgaccat tggctcaatg gtgaacgtca tccccggttt
    30961 cagcacgacg ttggtttcac gggagtcata gtgcagcacc tgcggttctt catggaagcc
    31021 gcgaccaata ccgtgtccgc aatattcacg aacgacggag aagccttctg cttcgacaaa
    31081 tttctgaatc gccgcaccga tttcgcgcag attaatgcct ggttttacca tgcgtagcgc
    31141 caggtacagg ctttcttgcg tgatgcggca caggcgttcg cccatgatgg tcggcttacc
    31201 gacgataaac attttcgagg tatcgccgtg gaaaccatct ttgattacgg tgacatcaat
    31261 gttaacgata tcgccatctt tcagcagctt agcatcgtcc gggataccgt ggcacaccac
    31321 ttcattaata gagatgcaaa cggatttcgg atagccgtga tagccgaggc aggcagaaac
    31381 cgcgtgttgt tcattaacaa tgtaatcatt acagatgcga tccagctcgc cggtgctgac
    31441 gcccggttta acatacggtt cgatcatctc cagcacttcg gcagccagtc ggccagcgac
    31501 gcgcattttt tcgatatctt ctggggtctt gattgagata gccattaatt ctgtccatca
    31561 gcgtcggtga taccgacaat atatatgtaa gtgccgtcaa tggtatcaca cccgggcaaa
    31621 ttgagaatca ttctgaattt cgccaaacgt gccactgaag gttttctata atagaaaatt
    31681 cgacgtctga tgctgtacac agcgccaaca attattggtg tccacgacgt atttgtggta
    31741 taaagcgcgc cggacttccg atccatttcg tatacacaga ctggacggaa gcgacaatct
    31801 cactttgtgt aacaacacac acgtatcggc acatattccg gggtgccctt tggggtcggt
    31861 aatatgggat acgtggaggc ataaccccaa cttttatata gaggttttaa tcatggcaac
    31921 tgtttccatg cgcgacatgc tcaaggctgg tgttcacttc ggtcaccaga cccgttactg
    31981 gaacccgaaa atgaagccgt tcatcttcgg tgcgcgtaac aaagttcaca tcatcaacct
    32041 tgagaaaact gtaccgatgt tcaacgaagc tctggctgaa ctgaacaaga ttgtttctcg
    32101 caaaggtaaa atccttttcg ttggtactaa acgcgctgca agcgaagcgg tgaaagacgc
    32161 tgctctgagc tgcgaccagt tcttcgtgaa ccatcgctgg ctgggcggta tgctgactaa
    32221 ctggaaaacc gttcgtcagt ccatcaaacg tctgaaagac ctggaaactc agtctcagga
    32281 cggtactttc gacaagctga ccaagaaaga agcgctgatg cgcactcgtg agctggagaa
    32341 actggaaaac agcctgggcg gtatcaaaga catgggcggt ctgccggacg ctctgtttgt
    32401 aatcgatgct gaccacgaac acattgctat caaagaagca aacaacttgg gtattccggt
    32461 atttgctatc gttgatacca actctgatcc ggacggtgtt gacttcgtta tcccgggtaa
    32521 cgacgacgca atccgtgctg tgaccctgta cctgggcgct gttgctgcaa ccgtacgtga
    32581 aggccgttct caggatctgg cttcccaggc ggaagaaagc ttcgtagaag ctgagtaata
    32641 aggcttgata actcccccaa aatagttcga gttgcagaaa ggcggcaagc tcgagaattc
    32701 ccgggagctt acatcagtaa gtgaccggga tgagcgagcg aagacaacgc atctgcggcg
    32761 cgaaatatga agggggagag cccttataga ccaggtagta cacgtttggt tagggggcct
    32821 gcatatggcc ccctttttca cttttatatc tgtgcggttt aatgccgggc agatcacatc
    32881 tccgaggatt ttagaatggc tgaaattacc gcatccctgg taaaagagct gcgtgagcgt
    32941 actggcgcag gcatgatgga ttgcaaaaaa gcactgactg aagctaacgg cgacatcgag
    33001 ctggcaatcg aaaacatgcg taagtccggt gctattaaag cagcgaaaaa agcaggcaac
    33061 gttgctgctg acggcgtgat caaaaccaaa atcgacggca actacggcat cattctggaa
    33121 gttaactgcc agactgactt cgttgcaaaa gacgctggtt tccaggcgtt cgcagacaaa
    33181 gttctggacg cagctgttgc tggcaaaatc actgacgttg aagttctgaa agcacagttc
    33241 gaagaagaac gtgttgcgct ggtagcgaaa attggtgaaa acatcaacat tcgccgcgtt
    33301 gctgcgctgg aaggcgacgt tctgggttct tatcagcacg gtgcgcgtat cggcgttctg
    33361 gttgctgcta aaggcgctga cgaagagctg gttaaacaca tcgctatgca cgttgctgca
    33421 agcaagccag aattcatcaa accagaagac gtatccgctg aagtggtaga aaaagaatac
    33481 caggtacagc tggatatcgc gatgcagtct ggtaagccga aagaaatcgc agagaaaatg
    33541 gttgaaggcc gcatgaagaa attcaccggc gaagtttctc tgaccggtca gccgttcgtt
    33601 atggaaccaa gcaaaactgt tggtcagctg ctgaaagagc ataacgctga agtgactggc
    33661 ttcatccgtt tcgaagtggg tgaaggcatc gagaaagttg agactgactt tgcagcagaa
    33721 gttgctgcga tgtccaagca gtcttaatta tcaaaaagga gccgcctgag ggcggcttct
    33781 ttttgtgccc atcttgtaaa ttcagctaac ccttgtgggg ctgcgctgaa aagcgacgta
    33841 caatgtcgct agtattaatt catttcaatc gttgacagtc tcaggaaaga aacatggcta
    33901 ccaatgcaaa acccgtctat aaacgcattc tgcttaagtt gagtggcgaa gctctgcagg
    33961 gcactgaagg cttcggtatt gatgcaagca tactggatcg tatggctcag gaaatcaaag
    34021 aactggttga actgggtatt caggttggtg tggtgattgg tgggggtaac ctgttccgtg
    34081 gcgctggtct ggcgaaagcg ggtatgaacc gcgttgtggg cgaccacatg gggatgctgg
    34141 cgaccgtaat gaacggcttg gcaatgcgtg atgcactgca ccgcgcctat gtgaacgctc
    34201 gtctgatgtc cgctattcca ttgaatggcg tgtgcgacag ctacagctgg gcagaagcta
    34261 tcagcctgtt gcgcaacaac cgtgtggtga tcctctccgc cggtacaggt aacccgttct
    34321 ttaccaccga ctcagcagct tgcctgcgtg gtatcgaaat tgaagccgat gtggtgctga
    34381 aagcaaccaa agttgacggc gtgtttaccg ctgatccggc gaaagatcca accgcaacca
    34441 tgtacgagca actgacttac agcgaagtgc tggaaaaaga gctgaaagtc atggacctgg
    34501 cggccttcac gctggctcgt gaccataaat taccgattcg tgttttcaat atgaacaaac
    34561 cgggtgcgct gcgccgtgtg gtaatgggtg aaaaagaagg gactttaatc acggaataat
    34621 tcccgtgatg gataaataag ggtaagattc cgcgtaagta tcgcgggggc gtaagtctgg
    34681 ttataaggcg ttattgttgc aggcagtttg gtcacggcca gcgcgcagca accggagcgt
    34741 acaaaagtac gtgaggatgg cgagcactgc tcggggccaa aatggcaaat aaaatagcct
    34801 aataatccag acgattaccc gtaatatgtt taatcagggc tatacttagc acacttccac
    34861 tgtgtgtgac tgtctggtct gactgagaca agttttcaag gattcgtaac gtgattagcg
    34921 atatcagaaa agatgctgaa gtacgcatgg acaaatgcgt agaagcgttc aaaacccaaa
    34981 tcagcaaaat acgcacgggt cgtgcttctc ccagcctgct ggatggcatt gtcgtggaat
    35041 attacggcac gccgacgccg ctgcgtcagc tggcaagcgt aacggtagaa gattcccgta
    35101 ctctgaaaat caacgtgttt gatcgttcaa tgtctccggc cgttgaaaaa gcgattatgg
    35161 cctccgatct cggcctgaac ccgaactctg cgggtagcga catccgtgtt ccgctgccgc
    35221 cgctgacgga agaacgtcgt aaagatctga ccaaaatcgt tcgtggtgaa gcagaacaag
    35281 cgcgtgttgc agtacgtaat gtgcgtcgtg acgcgaacga caaagtgaaa gcactgttga
    35341 aagataaaga gatcagcgaa gacgacgatc gccgttctca ggacgatgta cagaaactga
    35401 ctgatgctgc aatcaagaaa attgaagcgg cgctggcaga caaagaagca gaactgatgc
    35461 agttctgatt tcttgaacga caaaaacgcc gctcagtaga tccttgcgga tcggctggcg
    35521 gcgttttgct ttttattctg tctcaactct ggatgtttca tgaagcaact caccattctg
    35581 ggctcgaccg gctcgattgg ttgcagcacg ctggacgtgg tgcgccataa tcccgaacac
    35641 ttccgcgtag ttgcgctggt ggcaggcaaa aatgtcactc gcatggtaga acagtgcctg
    35701 gaattctctc cccgctatgc cgtaatggac gatgaagcga gtgcgaaact tcttaaaacg
    35761 atgctacagc aacaggggag ccgcaccgaa gtcttaagtg ggcaacaagc cgcttgcgat
    35821 atggcagcgc ttgaggaggt tgatcaggtg atggcagcca ttgttggtgc tgctgggctg
    35881 ttacctacgc ttgctgcgat ccgcgcgggt aaaaccattt tgctggccaa taaagaatca
    35941 ctggttacct gcggacgtct gtttatggac gccgtaaagc agagcaaagc gcaattgtta
    36001 ccggtcgata gcgaacataa cgctattttt cagagtttac cgcaacctat ccagcataat
    36061 ctgggatacg ctgaccttga gcaaaatggc gtggtgtcca ttttacttac cgggtctggt
    36121 ggccctttcc gtgagacgcc attgcgcgat ttggcaacaa tgacgcccga tcaagcttgc
    36181 cgtcatccga actggtcgat gggacgtaaa atttctgtcg attcggctac catgatgaac
    36241 aaaggtctgg aatacattga agcgcgttgg ctgtttaacg ccagcgccag ccagatggaa
    36301 gtgctgattc acccgcagtc agtgattcac tcaatggtgc gctatcagga cggcagtgtt
    36361 ctggcgcagc tgggggaacc ggatatgcgt acgccaattg cccacaccat ggcatggccg
    36421 aatcgcgtga actctggcgt gaagccgctc gatttttgca aactaagtgc gttgacattt
    36481 gccgcaccgg attatgagcg ttatccatgc ctgaaactgg cgatggaggc gttcgaacaa
    36541 ggccaggcag cgacgacagc attgaatgcc gcaaacgaaa tcaccgttgc tgcttttctt
    36601 gcgcaacaaa tccgctttac ggatatcgct gcgttgaatt tatccgtact ggaaaaaatg
    36661 gatatgcgcg aaccacaatg tgtggacgat gtgttatctg ttgatgcgaa cgcgagtgaa
    36721 gtcgccagaa aagaggtgat gcgtctcgca agctgaggat aatccggcta cagagagtcg
    36781 cgctatttgt tagcgtaggg cttcagtgat atagtctgcg ccatctgatc gtaagtagtt
    36841 ggctttataa ggtcaggtat gccgtggttt tacacggctt ttttttgtat aggcttcagt
    36901 attcctgagt accgtaaacc ctgtcaggga ataaaaaacg cgtgatgttg tctgctactc
    36961 aaccacttag cgaaaaattg ccagcgcatg gctgccgtca tgttgcgatc attatggacg
    37021 gcaatggtcg ctgggcaaaa aagcaaggga agattcgtgc ctttgggcat aaagccgggg
    37081 caaaatccgt ccgccgggct gtctcttttg cggccaacaa cggtattgag gcgttaacgc
    37141 tgtatgcctt tagtagtgaa aactggaacc gaccagcgca ggaagtcagt gcgttaatgg
    37201 aactgtttgt gtgggcgctc gatagcgaag taaaaagtct gcaccgacat aacgtgcgtc
    37261 tgcgtattat tggcgatacc agtcgtttta actcgcgttt gcaagaacgt attcgtaaat
    37321 ctgaagcgct aacagccgga aataccggtc tgacgctgaa tattgcggcg aactacggtg
    37381 gacgttggga tatagtccag ggagtcaggc aactggctga aaaggtgcag caaggaaacc
    37441 tgcaaccaga tcagatagat gaagagatgt taaaccagca tgtctgtatg catgaactgg
    37501 cccctgtaga tttagtaatt aggactgggg gagagcatcg cattagtaac tttttgcttt
    37561 ggcaaattgc ctatgccgaa ctttacttta cagatgttct ctggcccgat ttcgatgaac
    37621 aagactttga aggggcgtta aatgcctttg ctaatcgaga gcgtcgtttc ggcggcaccg
    37681 agcccggtga tgaaacagcc tgatgggggt cgcttttgct gaagtatcgc ctgatatctg
    37741 cttttgtgtt aatacccgtc gtcatcgcgg cgttgtttct gttgccgccg gtggggttcg
    37801 ccattgtaac gctggtggtc tgcatgctgg cagcgtggga atggggacag cttagcggtt
    37861 ttaccactcg ttcgcagcga gtatggttgg cggtgttatg cgggttattg ttggcgctga
    37921 tgctttttct gttgccggaa tatcaccgaa atattcatca accgctggtt gaaatctcac
    37981 tttgggcttc gttgggttgg tggattgtcg cgctattgct ggtgctgttt tacccaggtt
    38041 ccgcagcaat ctggcgtaac tctaaaacat tgcgccttat ttttggcgtg ctaaccattg
    38101 ttcccttctt ctggggcatg ctggcgttac gggcctggca ctatgacgag aatcattaca
    38161 gtggcgcaat atggctgctc tatgtcatga tcctggtatg gggcgctgac tccggcgcat
    38221 atatgtttgg caaattgttt ggtaaacata agctggcacc gaaggtttct ccgggtaaaa
    38281 cctggcaagg ctttataggt ggactcgcta ctgcagcggt aatctcatgg ggttatggca
    38341 tgtgggcgaa tctcgacgtc gctcccgtca ccttactcat ttgctctatt gtcgcagcgt
    38401 tagcctcagt gctcggcgat ctgaccgaga gtatgtttaa gcgtgaagca ggaattaagg
    38461 acagcggtca tttaattcca ggacacggtg gtattttaga tcgtattgat agcctgacgg
    38521 ctgcggtacc ggtctttgct tgcttgttgt tactggtatt caggacgctt taacggaagg
    38581 taatatgctg agttttctct gggatttggc ttcgttcatc gttgcactgg gtgtacttat
    38641 caccgtgcat gaatttggtc atttctgggt tgcccggcgt tgtggtgttc gcgttgagcg
    38701 tttctcaata gggtttggta aggcgctctg gcggcgaact gataagctcg gcaccgaata
    38761 tgttatcgcc ctgatcccgt tgggcggtta tgtcaaaatg ctggatgagc gcgcagaacc
    38821 ggtcgttccg gaactccgcc accatgcctt caataataaa tctgtcggcc aacgagcggc
    38881 gattattgcc gcaggtccgg ttgcaaactt catttttgct atctttgcct actggctggt
    38941 ttttattatt ggtgtgcctg gcgtacgtcc ggtggttggc gaaatagcag ccaattcgat
    39001 agctgcggaa gcacaaattg caccaggtac ggaactaaaa gccgtagatg gtatcgaaac
    39061 gcctgattgg gatgccgtgc gtttgcagtt ggtcgataaa attggcgatg aaagcaccac
    39121 cattacagta gcgccatttg acagcgacca acggcgggat gtaaagctcg atttacgtca
    39181 ctgggcgttt gagcctgata aagaagatcc ggtatcttcg ctggggattc gtcctcgtgg
    39241 gccgcaaatt gaacctgtac tggaaaatgt gcagccaaac tcggcggcaa gcaaggcagg
    39301 tttgcaagca ggcgacagga tcgttaaagt cgatggtcag cccttaacgc agtgggtgac
    39361 ctttgtgatg cttgtccggg ataacccggg taaatcctta gcgttagaaa tcgaaaggca
    39421 ggggagtccc ttgtctttga cattaatccc ggagagtaaa ccgggtaatg gtaaagcgat
    39481 tggttttgtc ggtattgagc cgaaagtcat tcctttgcca gatgagtata aagttgtacg
    39541 ccagtatggg ccgttcaacg ccatcgtcga agccacggac aaaacgtggc agctgatgaa
    39601 gctgacggtc agtatgctgg gaaaattgat caccggtgat gtgaaactga acaacctcag
    39661 tgggccgatc tctatcgcca agggggctgg gatgacagcg gaactcgggg tagtttatta
    39721 cctgccgttt cttgcgctta ttagcgtgaa cttagggata attaacctgt ttccgttgcc
    39781 cgtacttgac ggggggcatc tgctgttcct tgcgatcgaa aagatcaagg gcggaccggt
    39841 atccgagcgg gttcaagact tttgttatcg cattggctcg attctgctgg tgctgttaat
    39901 ggggcttgca cttttcaatg atttctctcg gttatgagag ttagttagga agaacgcata
    39961 ataacgatgg cgatgaaaaa gttgctcata gcgtcgctgc tgtttagcag cgccaccgta
    40021 tacggtgctg aagggttcgt agtgaaagat attcatttcg aaggccttca gcgtgtcgcc
    40081 gttggtgcgg ccctcctcag tatgccggtg cgtacaggcg acacggttaa tgatgaagat
    40141 atcagtaata ccattcgcgc tctgtttgct accggcaact ttgaggatgt tcgcgtcctt
    40201 cgtgatggtg atacccttct ggttcaggta aaagaacgtc cgaccattgc cagcattact
    40261 ttctccggta acaaatcggt gaaagatgac atgctgaagc aaaacctcga ggcttctggt
    40321 gtgcgtgtgg gcgaatccct cgatcgcacc accattgccg atatcgagaa aggtctggaa
    40381 gacttctact acagcgtcgg taaatatagc gccagcgtta aagctgtcgt gaccccgctg
    40441 ccgcgcaacc gtgttgacct aaaactggtg ttccaggaag gtgtgtcagc tgaaatccag
    40501 caaattaaca ttgttggtaa ccatgctttc accaccgacg aactgatctc tcatttccaa
    40561 ctgcgtgacg aagtgccgtg gtggaatgtg gtaggcgatc gtaaatacca gaaacagaaa
    40621 ctggcgggcg atcttgaaac cctgcgcagc tactatctgg accgcggtta tgcccgtttc
    40681 aacatcgact ctacccaggt cagtctgacg ccagataaaa aaggtattta cgtcacggtg
    40741 aacatcaccg aaggcgatca gtacaagctt tctggcgttg aagtgagcgg caaccttgcc
    40801 gggcactccg ctgaaattga gcaactgact aagatcgagc cgggcgagct gtataacggc
    40861 actaaagtga ccaaaatgga agatgacatt aaaaagcttc tcggtcgcta tggttatgcc
    40921 tatccgcgcg tacagtcgat gcctgaaatt aacgatgccg acaaaaccgt taaattacgc
    40981 gtgaacgttg atgcgggtaa ccgtttctac gtgcgtaaga tccgttttga aggtaacgat
    41041 acctcgaaag atgccgtcct gcgtcgcgaa atgcgtcaga tggaaggtgc atggttgggg
    41101 agcgatctgg tcgatcaggg taaggagcgt ctgaatcgtc tgggcttctt tgaaactgtc
    41161 gataccgata cccaacgtgt tccgggtagc ccggaccagg ttgatgtagt ctacaaggtg
    41221 aaagaacgta acactggtag cttcaacttc gggattggtt acggtactga aagtggcgtg
    41281 agcttccagg ctggcgtgca acaggataac tggttaggta caggttatgc tgttggtatc
    41341 aacgggacca aaaacgatta ccagacctat gctgaactgt cggtaaccaa cccgtacttc
    41401 accgtagatg gcgtaagcct cggtggtcgt ctcttctata atgacttcca ggcagatgac
    41461 gccgacctgt ccgactatac caacaagagt tatggtacag acgtgacgtt gggcttcccg
    41521 attaacgaat ataactcgct gcgtgcaggt ctgggttatg tacataactc cctgtccaac
    41581 atgcagcctc aggttgcgat gtggcgttat ctgtactcta tgggtgaaca tccgagcacc
    41641 tctgatcagg ataacagctt caaaacggac gacttcacgt tcaactatgg ttggacctat
    41701 aacaagcttg accgtggtta cttcccgaca gatggttcac gtgtcaacct gaccggtaaa
    41761 gtgaccattc ctggatcgga taacgaatac tacaaagtga cgttagacac ggcgacttat
    41821 gtgccgatcg atgacgatca caaatgggtt gttctgggtc gtacccgctg gggttatggt
    41881 gatggtttag gcggcaaaga gatgccgttc tacgagaact tctatgccgg tggttccagc
    41941 accgtgcgtg gcttccagtc caataccatt ggtccgaaag cagtttactt cccgcatcag
    42001 gccagtaatt atgatccgga ctatgattac gaatgtgcga ctcaggacgg cgcgaaagac
    42061 ctgtgtaaat cggatgatgc tgtaggcggt aacgccatgg cggttgccag cctcgagttc
    42121 atcaccccga cgccgtttat tagcgataag tatgctaact cggttcgtac ttcattcttc
    42181 tgggatatgg gtaccgtttg ggatacaaac tgggattcca gccaatattc tggatatccg
    42241 gactatagtg atccaagcaa tatccgtatg tctgcgggta tcgcattaca atggatgtcc
    42301 ccattggggc cgttggtgtt ctcctacgcc cagccgttca aaaagtacga tggagacaag
    42361 gcagaacagt tccagtttaa catcggtaaa acctggtaag tgttctccac aaaggaatgt
    42421 agtggtagtg tagcgatgac tttaggcgat caatataaga tcgccgggcc acgcaaagaa
    42481 ctgcaccctc cggtgcaaat gggatggtaa ggagtttatt gtgaaaaagt ggttattagc
    42541 tgcaggtctt ggtttagcac tggcaacttc tgctcaggcg gctgacaaaa ttgcaatcgt
    42601 caatatgggc agcctgttcc agcaggtagc gcagaaaacc ggtgtttcta acacgctgga
    42661 aaatgagttc aaaggccgtg ccagcgaact gcagcgtatg gaaaccgatc tgcaggctaa
    42721 aatgaaaaaa ctgcagtcca tgaaagcggg cagcgatcgc accaaactgg aaaaagacgt
    42781 gatggctcag cgccagactt ttgctcagaa agcgcaggct tttgagcagg atcgcgcacg
    42841 tcgttccaac gaagaacgcg gcaaactggt tactcgtatc cagactgctg tgaaatccgt
    42901 tgccaacagc caggatatcg atctggttgt tgatgcaaac gccgttgctt acaacagcag
    42961 cgatgtaaaa gacatcactg ccgacgtact gaaacaggtt aaataagtaa tgccttcaat
    43021 tcgactggct gatttagcgc agcagttgga tgcagaacta cacggtgatg gcgatatcgt
    43081 catcaccggc gttgcgtcca tgcaatctgc acaaacaggt cacattacgt tcatggttaa
    43141 cccaaaatac cgtgagcatt taggcttgtg ccaggcgtcc gcggttgtca tgacccagga
    43201 cgatcttcct ttcgcgaaaa gtgccgcgct ggtagtgaag aatccctacc tgacttacgc
    43261 gcgcatggcg caaattttag ataccacgcc gcagcccgcg cagaacattg cacccagtgc
    43321 ggtgatcgac gcgacggcga agctgggtaa caacgtatcg attggcgcta acgcggtgat
    43381 tgagtccggc gttgaactgg gcgataacgt gattatcggt gccggttgct tcgtaggtaa
    43441 aaacagcaaa atcggtgcag gttcgcgtct ctgggcgaac gtaaccattt accatgagat
    43501 ccagatcggt cagaattgcc tgatccagtc cggaacagtg gtaggcgcag acggctttgg
    43561 ttatgccaac gatcgtggta actgggtgaa gatcccacag attggtcgcg taattattgg
    43621 cgatcgcgtg gagatcggtg cctgcacaac catcgatcgc ggcgcgctgg atgacactgt
    43681 tattggcaat ggcgtgatca ttgataacca gtgccagatt gcacataacg tcgtgattgg
    43741 cgacaatacg gcggttgccg gtggcgtcat tatggcgggc agcctgaaaa ttggtcgtta
    43801 ctgcatgatc ggcggagcca gcgtaatcaa cgggcatatg gaaatatgcg acaaagtgac
    43861 ggttacgggc atgggtatgg tgatgcgtcc catcactgaa ccaggcgtct attcctcagg
    43921 cattccgctg caacccaaca aagtctggcg caaaaccgct gcactggtga tgaacattga
    43981 tgacatgagc aagcgtctga aatcgcttga gcgcaaggtt aatcaacaag actaacgttc
    44041 catcttttgt tcgccaaact ttacggcctg tctcattctt acgattgcgg caggccgtgt
    44101 tattattgtc gtttcttata ttttgacagg aagagtatct tgactactaa cactcatact
    44161 ctgcagattg aagagatttt agaacttctg ccgcaccgtt tcccgttctt actggtggat
    44221 cgcgtgctgg attttgaaga aggtcgtttt ctgcgcgcag taaaaaatgt ctctgtcaat
    44281 gagccattct tccagggcca tttccctgga aaaccgattt tcccgggtgt gctgattctg
    44341 gaagcaatgg cacaggcaac aggtattctg gcgtttaaaa gcgtaggaaa actggaaccg
    44401 ggtgagctgt actacttcgc tggtattgac gaagcgcgct tcaagcgccc ggtcgtgcct
    44461 ggcgatcaaa tgatcatgga agtcactttc gaaaaaacgc gccgcggcct gacccgtttt
    44521 aaaggggttg ctctggtcga tggtaaagta gtttgcgaag caacgatgat gtgtgctcgt
    44581 agccgggagg cctgatacgt gattgataaa tccgcctttg tgcatccaac cgccattgtg
    44641 gaagagggcg cgtcaattgg cgcgaacgca cacattggtc ctttttgtat cgttggaccc
    44701 catgtcgaaa ttggtgaggg taccgtactg aaatctcacg ttgtcgtgaa tggtcatact
    44761 aaaattggcc gcgataatga gatttatcag tttgcctcca tcggcgaagt taaccaggat
    44821 ttgaaatatg ctggcgaacc gacccgtgtg gaaatcggcg atcgtaaccg cattcgcgaa
    44881 agcgtcacca ttcatcgtgg cacagtccag ggcggtggat tgacgaaggt gggcagcgac
    44941 aacttactga tgatcaacgc gcacattgcg cacgattgta cggtaggtaa ccgctgtatt
    45001 ctcgccaaca acgcaacgct ggcgggtcac gtatcggttg acgacttcgc gatcatcggc
    45061 ggcatgaccg cagtccatca gttctgcatc attggtgcgc atgtgatggt tggcggctgc
    45121 tccggtgtgg cgcaggacgt ccctccttat gttattgcgc agggtaacca cgcaacgccg
    45181 ttcggtgtca atatcgaagg gctgaagcgc cgcggattca gccgtgaggc gattaccgct
    45241 atccgcaatg cgtataagct aatttatcgt agcggtaaaa cgctcgatga agtgaaaccg
    45301 gaaattgctg aactggcgga aacatatccg gaagtgaaag cctttaccga tttctttgca
    45361 cgctcaacgc gcggtctgat tcgttaatga ctgaccagcg tccattaacg attgccctgg
    45421 tcgccggaga aacctccggc gatatcctgg gggccggttt aatccgcgct ctgaaagaac
    45481 gtgtgcccaa cgcccgcttt gttggtgttg ccgggccacg aatgcaggct gaaggctgcg
    45541 aagcctggta cgaaatggaa gaactggcgg tgatgggcat tgttgaagtg ctcggtcgtc
    45601 tgcgtcgctt actgcatatt cgtgccgatc tgacaaagcg ttttggcgaa ctgaagccag
    45661 atgtttttgt tggtattgat gcgcccgact tcaatattac tcttgaaggt aacctcaaaa
    45721 agcagggtat caaaaccatt cattacgtca gtccatccgt ctgggcgtgg cgacagaaac
    45781 gcgttttcaa aataggcaga gccaccgatc tggtgctcgc atttctgcct ttcgaaaaag
    45841 cgttttatga caaatacaat gtaccgtgcc gctttatcgg tcataccatg gctgatgcca
    45901 tgccgttaga tccagataaa aatggtgccc gtgatgtgct ggggatccct tacgatgccc
    45961 actgtctggc attgttgccg ggcagccgtg gtgcagaagt cgaaatgctt agtgccgatt
    46021 tcttgaaaac tgcccagctt ttgcgccaga catatccgga tctcgaaatc gtggtgccgc
    46081 tggtgaatgc caaacgccgc gagcagtttg aacgcatcaa agctgaagtc gcgccagacc
    46141 tgtcggttca tttgctggat ggaatgggcc gtgaggcgat ggtcgccagc gatgcggcac
    46201 tactggcgtc ggggacggca gccctggagt gtatgctggc gaaatgcccg atggtggtgg
    46261 gatatcgcat gaagcctttt accttctggt tggcgaagcg gctggtgaaa actgattatg
    46321 tctcgctgcc aaatctgctg gcgggcagag agttagtcaa agagttattg caggaagagt
    46381 gtgagccgca aaaactggct gcggcgctgt taccgctgtt ggcgaacggg aaaaccagcc
    46441 acgcgatgca cgataccttc cgtgaactgc atcagcagat ccgctgcaat gccgatgagc
    46501 aggcggcaca agccgttctg gagttagcac aatgatcgaa tttgtttatc cgcacacgca
    46561 gctggttgcg ggtgtggatg aagtcggacg cgggccgtta gttggcgcgg tcgtcaccgc
    46621 tgcggtgatc cttgacccgg cgcgcccgat tgccgggctg aatgattcca aaaagctgag
    46681 cgaaaaacgc cgtctggcgc tctatgaaga gatcaaagag aaagcgttga gctggagtct
    46741 gggccgcgcg gaaccccacg aaatcgacga gctgaacatt cttcatgcga ccatgctggc
    46801 gatgcagcgt gccgtcgctg ggctgcatat tgcgccggaa tatgtgttga ttgatggtaa
    46861 ccgctgcccg aaattaccga tgcctgcgat ggctgtggtg aaaggggata gccgcgtacc
    46921 agaaatcagt gccgcgtcta tcctggcgaa agtgacgcgt gacgccgaaa tggcggcgct
    46981 ggatattgtt ttcccgcaat atggttttgc ccaacataaa gggtatccaa ccgcttttca
    47041 tctggaaaaa ctggctgaat atggcgcgac tgaacatcat cgtcgcagct ttgggcctgt
    47101 caaacgcgca ctgggacttg cgtcctgatt cttgtgtcga gattaagtaa accggaatct
    47161 gaagatgtct gaaccacgtt tcgtacacct gcgggtgcac agcgactact cgatgatcga
    47221 tggcttggcc aaaaccgcac cgctggtaaa aaaggcggcg gcgttgggta tgccagcact
    47281 ggcgatcacc gatttcacca acctttgcgg tctggtgaag ttctacggag cgggacatgg
    47341 cgcagggatt aagcctatcg tcggggcaga ttttaatgtc cagtgcgacc tgctgggtga
    47401 tgagttaacc cacctgacgg tactggcggc gaacaatacc ggctatcaga atctggcgtt
    47461 gctgatctca aaagcgtatc agcgcgggta cggtgccgcc gggccgatca tcgatcgcga
    47521 ctggcttatc gaattaaacg aagggttgat ccttctttcc ggcgggcgca tgggcgacgt
    47581 cggacgcagt cttttgcgtg gtaacagcgc gctggtagat gagtgtgtcg cgttttatga
    47641 agaacacttc ccggatcgct attttctcga gctgatccgc accggcaggc cggatgaaga
    47701 aagctatctg cacgcggcgg tggaactggc ggaagcgcgc ggtttgcccg tcgtggcgac
    47761 taacgatgtg cgctttatcg acagcagcga ctttgacgca cacgaaatcc gcgtcgcgat
    47821 ccacgacggc tttaccctcg acgatcctaa acgcccgcgt aactattcgc cgcagcaata
    47881 tatgcgtagc gaagaggaga tgtgtgagct gtttgccgac atcccagaag cccttgccaa
    47941 caccgttgag atcgccaaac gctgtaacgt aactgtgcgt cttggtgaat acttcctgcc
    48001 gcagttcccg accggggaca tgagcaccga agattatctg gtcaagcgtg caaaagaagg
    48061 cctggaagag cgtctggcat ttttattccc tgacgaggaa gaacgtctta aacgccgccc
    48121 ggaatatgac gaacgtctgg agactgaact tcaggttatc aaccagatgg gcttcccggg
    48181 ctacttcctc atcgttatgg aatttatcca gtggtcgaaa gataacggcg taccggtagg
    48241 gccaggccgt ggctccggtg cgggttcact ggtggcctac gcgctgaaaa tcaccgacct
    48301 cgatccgctg gaatttgacc tgctgttcga acgtttcctt aacccggaac gtgtctccat
    48361 gcctgacttc gacgttgact tctgtatgga gaaacgcgat caggttatcg aacacgtggc
    48421 ggacatgtac ggtcgtgatg cggtatcgca gattatcacc ttcggtacga tggcggcgaa
    48481 agcggtgatc cgcgacgtag gccgcgtgct ggggcatccg tacggctttg tcgaccgtat
    48541 ctcgaaactg atcccgcccg atccggggat gacgctggcg aaagcgtttg aagccgagcc
    48601 gcagctgccg gaaatctacg aagcggatga agaagttaag gcgctgatcg acatggcgcg
    48661 caaactggaa ggggtcaccc gtaacgccgg taagcacgcc ggtggggtgg ttatcgcgcc
    48721 gaccaaaatt accgattttg cgccgcttta ttgcgatgaa gagggcaaac atccggtcac
    48781 ccagtttgat aaaagcgacg ttgaatacgc cggactggtg aagttcgact tccttggttt
    48841 gcgtacgctc accatcatca actgggcgct ggagatgatc aacaagcggc gggcgaagaa
    48901 tggcgagcca ccgctggata tcgccgcgat cccgctggat gacaagaaaa gcttcgacat
    48961 gctgcaacgc tcggaaacca cggcggtatt ccagcttgaa tcgcgcggca tgaaggacct
    49021 gatcaagcgt ctgcaacctg actgcttcga agatatgatc gcactggtgg cactgttccg
    49081 ccctggtccg ttgcagtcag ggatggtgga taactttatc gaccgtaaac atggtcgtga
    49141 agagatctcc tatccggacg tgcagtggca gcatgaaagc ctgaaatcgg tactggagcc
    49201 aacctacggc attatcttgt atcaggaaca ggtcatgcag attgcccagg tgctttctgg
    49261 ttataccctc ggtggcgcgg atatgctgcg ccgtgcgatg ggtaagaaaa agccggaaga
    49321 gatggctaag cagcgttctg tatttgctga aggtgcagaa aagaacggaa tcaacgccga
    49381 actggcgatg aaaatcttcg acctggtgga gaaattcgcg ggttacggat ttaacaaatc
    49441 gcactctgcg gcctatgctt tggtgtcata tcaaacgtta tggctgaaag cgcactatcc
    49501 ggcggagttt atggcggcgg taatgaccgc cgatatggac aacaccgaga aggtggtggg
    49561 cctggtggat gagtgctggc ggatggggct gaaaatcctg ccaccagata taaactccgg
    49621 tctttaccat ttccacgtca acgacgatgg cgaaatcgtg tatggtattg gcgccatcaa
    49681 aggggtaggt gaaggtccga ttgaggccat catcgaagcc cgtaataaag gcggctactt
    49741 ccgcgaactg tttgatctct gcgcccgtac cgacaccaaa aagttaaacc ggcgggtgct
    49801 ggaaaaactg atcatgtccg gggcgtttga ccgtcttggg ccacaccgcg cggcgctgat
    49861 gaactcgctg ggtgatgcgt taaaagcggc agatcaacac gcgaaagcgg aagctatcgg
    49921 tcaggccgat atgttcggcg tgctggccga agagccggaa caaattgaac aatcctacgc
    49981 cagctgccaa ccgtggccgg agcaggtggt attagatggg gaacgtgaaa cgttaggcct
    50041 gtacctgacc ggacacccta tcaaccagta tttaaaagag attgagcgtt atgtcggagg
    50101 cgtaaggctg aaagacatgc acccgacaga acgtggtaaa gtcatcacgg ctgcggggct
    50161 cgttgttgcc gcgcgggtta tggtcaccaa gcgcggcaat cgtatcggta tctgcacgct
    50221 ggatgaccgt tccgggcggc tggaagtgat gttgtttact gacgccctgg ataaatacca
    50281 gcaattgctg gaaaaagacc gcatacttat cgtcagcgga caggtcagct ttgatgactt
    50341 cagcggtggg cttaaaatga ccgctcgcga agtgatggat attgacgaag cccgggaaaa
    50401 atatgctcgc gggcttgcta tctcgctgac ggacaggcaa attgatgacc agcttttaaa
    50461 ccgactccgt cagtctctgg aaccccaccg ctctgggaca attccagtac atctctacta
    50521 tcagagggcg gatgcacgcg cgcggttgcg ttttggcgcg acgtggcgtg tctctccgag
    50581 cgatcgttta ttaaacgatc tccgtggcct cattggttcg gagcaggtgg aactggagtt
    50641 tgactaatac aggaatacta tgagtctgaa tttccttgat tttgaacagc cgattgcaga
    50701 gctggaagcg aaaatcgatt ctctgactgc ggttagccgt caggatgaga aactggatat
    50761 taacatcgat gaagaagtgc atcgtctgcg tgaaaaaagc gtagaactga cgcgtaaaat
    50821 cttcgccgat ctcggtgcat ggcagattgc gcaactggca cgccatccgc agcgtcctta
    50881 taccctggat tacgttcgcc tggcatttga tgaatttgac gaactggctg gcgaccgtgc
    50941 gtatgcagac gataaagcta tcgtcggtgg tatcgcccgt ctcgatggtc gtccggtgat
    51001 gatcattggt catcaaaaag gtcgtgaaac caaagaaaaa attcgccgta actttggtat
    51061 gccagcgcca gaaggttacc gcaaagcact gcgtctgatg caaatggctg aacgctttaa
    51121 gatgcctatc atcaccttta tcgacacccc gggggcttat cctggcgtgg gcgcagaaga
    51181 gcgtggtcag tctgaagcca ttgcacgcaa cctgcgtgaa atgtctcgcc ttggcgtacc
    51241 ggtagtttgt acggttatcg gtgaaggtgg ttctggcggt gcgctggcga ttggcgtggg
    51301 cgataaagtg aatatgctgc aatacagcac ctattccgtt atctcgccgg aaggttgtgc
    51361 gtccattctg tggaagagcg ccgacaaagc gccgctggcg gctgaagcga tgggtatcat
    51421 cgctccgcgt ctgaaagagc tgaaactgat cgactccatc atcccggaac cgctgggcgg
    51481 cgctcaccgt aacccggaag cgatggcggc atcgttgaaa gcgcaactgc tggcggatct
    51541 ggccgatctc gatgtgttaa gcactgaaga tttaaaaaat cgtcgttatc agcgcctgat
    51601 gagctacggt tacgcgtaat tcgcaaaagt tctgaaaaag ggtcacttcg gtggcccttt
    51661 tttatcgcca cggtttgagc aggctatgat taaggaagga ttttccagga ggaacacatg
    51721 aacatcattg ccattatggg accgcatggc gtcttttata aagatgagcc catcaaagaa
    51781 ctggagtcgg cgctggtggc gcaaggcttt cagattatct ggccacaaaa cagcgttgat
    51841 ttgctgaaat ttatcgaaca taaccctcga atttgcggcg tgatttttga ctgggatgag
    51901 tacagtctcg atttatgcag cgatatcaat caacttaatg aatatctccc gctttatgcc
    51961 ttcatcaaca cccactcgac gatggatgtc agcgtgcagg atatgcggat ggcgctctgg
    52021 ttttttgaat atgcgctggg gcaggcggaa gatatcgcca ttcgtatgcg tcagtacacc
    52081 aacgaatatc ttgataacat tacgccgccg ttcacgaaag ccttgtttac ctacgtcaaa
    52141 gagcggaagt acaccttttg tacgccgggg catatgggcg gcaccgcata tcaaaaaagc
    52201 ccggttggct gtctgtttta tgattttttc ggcgggaata cccttaaggc tgatgtctct
    52261 atttcggtca ccgagcttgg ttcgttgctt gaccacaccg ggccacacct ggaagcggaa
    52321 gagtacatcg cgcggacttt tggcgcggaa cagagttata tcgttaccaa cggaatatcg
    52381 acgtcgaaca aaattgtggg tatgtacgcc gcgccatccg gcagtacgct gttgatcgac
    52441 cgcaactgcc ataaatcact ggcgcatctg ttgatgatga acgatgtagt gccagtctgg
    52501 ctgaaaccga cgcgtaatgc gttggggatt ctggggggga tcccgcgccg tgaatttact
    52561 cgcgacagca tcgaagagaa agtcgctgcc actacgcaag cacaatggcc ggttcatgcg
    52621 gtgatcacca actccaccta tgatggtttg ctctacaaca ccgactggat caaacagacg
    52681 ctggatgtcc cgtcgatcca cttcgattcc gcctgggtgc cgtatactca ttttcatcca
    52741 atctaccagg gtaaaagtgg tatgagcggc gagcgtgttg cgggaaaagt tttcttcgaa
    52801 acgcaatcga cccacaaaat gctggcggcg ttatcgcagg cttcactgat ccacattaaa
    52861 ggcgagtatg acgaagaggc gtttaacgaa gcctttatga tgcataccac cacctcgccc
    52921 agttatccca ttgttgcttc ggttaagacg gcggcggcga tgctgcgtgg taatccgggc
    52981 aaacggctga ttaaccgttc ggtggagcgg gcgctgcatt ttcgcaaaga ggtccagcgg
    53041 ctgcgggaag agtctgacgg ttggtttttc gatatctggc aaccgccgca ggtggatgaa
    53101 gccgaatgca ggcccgttgc gcctggcgaa caatggcacg gctttaacga tgcggatgcc
    53161 gatcatatgt ttctcgatcc ggttaaagtc actattttga caccggggat ggacgaacag
    53221 ggcaatatga gtgaggaggg gatcccggcg gcgctggtag caaaattcct cgacgaacgt
    53281 gggatcgtag tagagaaaac cggcccttat aacctgctgt ttctctttag tattggcatc
    53341 gataaaacca aagcaatggg attattgcgt gggttgacgg aatttaagcg ctcttacgat
    53401 ctcaacctgc ggatcaaaaa tatgctgccc gatctctatg ctgaagatcc cgatttctac
    53461 cgcaatatgc gtattcagga tctggcgcaa gggatccata agctgattcg taaacacgat
    53521 ctttccggtt tgatgttgcg ggcattcgat actttgccgg aaatgatcat gacgccacat
    53581 caggcatggc agagacagat taaaggtgaa gtagaaacca ttgcgccgga acaactggtc
    53641 ggtagagtat cggcaaatat gatcctgcct tatccaccgg gcgtaccgct gttgatgcca
    53701 ggagaaatgc tgaccaaaga gagccgcacg gtactcgatt ttctactgat gctttgttcc
    53761 gtagggcaac attaccccgg ttttgaaacg gatattcacg gcgcgaaaca ggacgaagac
    53821 ggcgtttacc gcgtacgagt cctaaaaatg gcgggataac ttgccagagc ggcttccggg
    53881 cgagtaacgt gctgttaaca aataaaggag acgttatgct gggtttaaaa caggttcacc
    53941 atattgcgat tattgcgacg gattatgcgg tgagcaaagc tttctactgc gatattcttg
    54001 gtttcacgct gcaaagcgaa gtctatcgcg aagcgcgcga ctcgtggaaa ggggatttgg
    54061 cgcttaatgg gcaatatgtg attgagcttt tctcttttcc gttcccgccg gagcgcccca
    54121 gccgaccgga agcttgcggc ctgcgtcatc tggcttttag cgttgatgac atcgatgcgg
    54181 cagtggcgca ccttgaaagc cataacgtga agtgtgaagc catccgtgtc gatccataca
    54241 cgcaaaaacg cttcaccttc tttaacgatc cggacgggct gccgttggaa ctgtatgagc
    54301 agtaaggctt gtcatcgccg catttgcccg gtaacgtgcc gggcattgct actgtaaaat
    54361 cgcaccatca tgacactcac gctcaataga caacttctca cctcacgcca gattctggtg
    54421 gcctttagcg gcgggcttga ctccaccgtt ctgctgcatc agttggtgca gtggcggacg
    54481 gaaaatccgg gcgtcactct gcgcgctatt catgtgcatc acggtttaag tgccaatgcc
    54541 gatgcctggg ttacgcattg cgaaaacgtc tgccaacagt ggcaggtgcc gctggtggtc
    54601 gaacgcgtac aacttgcgca agaggggctg gggattgagg cccaggcgcg gcaggcacgt
    54661 tatcaggcat ttgcccgtac cctattgccc ggcgaagtgc tggtcaccgc gcaacatctc
    54721 gacgatcaat gtgaaacctt tctgctggcg ctaaaacgcg gtagcggccc tgccgggctt
    54781 tcggctatgg cggaagtttc ggagtttgct ggaacacggc ttatccgccc gttgcttgcc
    54841 cgcacgcggg gggaactgga gcagtgggca ttggcgcatg gtttacgctg gattgaagac
    54901 gaaagtaatc aggacgactc atacgatcgt aactttctgc gcctgcgcgt agtgccgtta
    54961 ttgcagcagc gttggccgca ttttgcggaa gcaacggccc gcagcgccac actttgtgct
    55021 gaacaagaga gtctgttgga tgaactgctg gcggatgatt tagcacactg ccaaacgtcg
    55081 caggggacgc tgcagattgc gccaatgctg gcgatgagtg atgcccgtcg cgcggcgatt
    55141 attcgccgct ggctggcagg gcagaatgca ccgatgcctt cccgcgacgc gctggtgcgc
    55201 atctggcagg aagtggcgct ggcgcgggaa gatgcttccc cctgtttacg tttgggcgcc
    55261 tttgaaatcc gccgctatca gtcgcaactg tggtggatta aatccgtcac cgggcaaagc
    55321 gaaaccattg tgctgtggca gacgtggcta caaccgctgg aattaccggc ggggctggga
    55381 accgtacagc ttactgcggg aggcgatatt cgccctccgc gtgcagacga agcggtcagc
    55441 gtgcgtttca aagcgccagg attgctgcat attgtcgggc gcaacggcgg acgtaagctg
    55501 aagaaaatct ggcaagagtt aggcgtgccg ccgtggctac gtgacaccac gccactgctg
    55561 ttttatggag aaacgctgat tgcggcggca ggggtatttg tgacgcaaga aggtgtggct
    55621 gaaggtgaga atggcgtaag ttttgtctgg cagaaaacgc ttagttaagt gaaagccgga
    55681 taagacgcat caaacgtcgc atccggcgaa agtcaatcag gattcgctta ccaccaccgt
    55741 accgatttca gggtggctaa agctggtaat tttatccaga cgcagctcgc gggtttcgcc
    55801 agcggcctcg acgaccagat actccacatt tttgcgggag actaaatcac tggcttttgc
    55861 ctgcaatttt tcgccatctt tcagctcaag tgtcagcatt aaatgatgct ggcaggcgag
    55921 ctcaagatta tcgtaatcat cacaattaat tggttgatac gtatcattca ttgacataat
    55981 cgctcaccag taagtttgcc gcagcgtatg ctgctttttc cctgacagcc tcagaaaggg
    56041 cgtcgtcggc agccatttca ttcagcactt tcaaaacgca gcccagcgcg tccggaacgt
    56101 atcctaagtc tccgctggcg atttccgcgt accgcttgcg tattaactca caatattttt
    56161 ccacatgccc tcctgtcagc actctgactt aaccgtggat gcaagtctaa gcctacgaag
    56221 ataaactctg tttcgcaagg tgactatacc acactcattt ctgcaatatc agcgccgcaa
    56281 ctgcacgtat tccgttacaa tggcctcctg attcgaaagg agttttctta tggcgcttaa
    56341 agcgacaatt tataaagcga cggttaatgt ggccgatctc gaccgcaacc agtttctcga
    56401 tgcctctctg acgctggcgc gccacccttc agaaacccag gagcgtatga tgctgcgctt
    56461 gctggcgtgg ctgaaatatg ctgatgaacg tctgcaattt acccgtggtt tgtgtgccga
    56521 tgatgagccg gaagcgtggc tgcgtaacga tcacctgggc attgatttgt ggattgagct
    56581 ggggctgccg gatgagcgac gaattaagaa agcctgcacc caggccgcag aagtggcgct
    56641 gtttacctat aatagtcggg cggcgcaaat ctggtggcag caaaatcaga gcaaatgtgt
    56701 gcagtttgcc aatctttccg tctggtatct ggacgatgaa caactggcga aagtaagcgc
    56761 ctttgccgat cgtaccatga cgctgcaggc aacgattcag gatggtgtta tctggttatc
    56821 ggatgataag aataatctgg aagttaactt aaccgcctgg caacaacctt catgattgtg
    56881 atttcccgac atgttgctat tcccgatggt gagcttgaga tcaccgccat tcgtgcgcag
    56941 ggcgctggcg ggcagcatgt taataagacc tcaacggcta ttcatctgcg ttttgacatt
    57001 cgggcgtcca gcctgccaga gtattacaaa gagcgtctgc tcgccgccag ccatcatttg
    57061 atcagcagtg atggcgtgat tgtcattaag gcacaggaat accgcagtca ggaactgaac
    57121 cgcgaagccg ctctggcccg gctggtggca gtgattaaag atttaacaaa agaacaaaaa
    57181 gcccgacgac ccacgcggcc cacccgtgca tcgaaagagc gcaggctggc atcgaaagca
    57241 caaaaatcaa gcgtgaaggc gatgcgcggc aaagtacgca gcggtcggga ataaaaagaa
    57301 ggaatggatg gtgaaaaaag cgatagtgac agcgatggct gtaatcagcc tctttactct
    57361 gatgggatgt aataatcggg ccgaagtcga tacgctttct ccggcgcagg ctgccgaact
    57421 gaaaccgatg ccgcaaagtt ggcgcggcgt gctaccgtgt gccgattgcg aaggaatcga
    57481 aatctctctg ttcctcgaaa aagacggaac atgggtgatg aatgagcgtt atctcggtgc
    57541 tcgtgaagaa ccttcctcct tcgcttccta cggtacatgg gcgcgaaccg ctgacaagct
    57601 ggtattaacc gacagcaaag gtgaaaagtc atattatcgg gcgaaaggcg atgcgctgga
    57661 gatgctcgat cgtgaaggca atccaattga atcgcagttc aactatacgc tggaaccggc
    57721 acaatccagt ttacctatga cgccgatgac cctgcgaggc atgtattttt atatggctga
    57781 tgcggcgacc ttcactgatt gcgcgaccgg aaaacgtttc atggtagcga ataacgcaga
    57841 gctggagcgt agctacctgg ctgcgcgcgg tcacagtgaa aaaccggtgt tactgtcagt
    57901 agaaggtcac tttacgcttg aggctaatcc ggatactggt gcgccaacca aagtattagc
    57961 gcccgatacg gcgggtaaat tttacccgaa taaggattgc agtagtttgg ggcagtaaac
    58021 ctgcttaagg cagaaacaaa cgcaaaattg cctgatgcgc tgcgcttatc aggcctacag
    58081 catataatgc aacattttga attagcacga ttttgtaggc cggataaggc gtttacgccg
    58141 catccggcat tttgtgcacg aggctggctt tatcactgag tcagcccaac aaagcgaccc
    58201 gccttgatat aaatcccagg cttcaggtgc ccaacatact gtaactgcgt ttctggctta
    58261 aacgcagaga catcaccaat acgcatatgc atcagatcgg cagggctaat ccagcccgat
    58321 tgcgccagtg taagtggatg cccggatttg gcaaatgcat cagtgacaaa ttccgaacaa
    58381 aaccacgact ttttgtctcc ttcgcacaca ctgcttaact gcgctttcgc caggtcgctg
    58441 acgcactgtt ggcgaaaatc ctcggagaac ggattcagtg agcacatctg gcgagtcacc
    58501 ataaagggaa taaattcgac aatgccgcga tagttataac cgctatcttt gattttattg
    58561 gcaaacgcgg tgatatctgt ggcttgttgc ggggtaagat ccgggactcg taagacgaaa
    58621 agcttatcac tatgcttcat cgctttttta agggaaacaa tctggacgcc agcgcctgtc
    58681 gcttctgcaa cgttattatc acccagaaag attgcaacgt gactcacaga ggaagtgctg
    58741 aagacgcgga ttccaaatga ggttacccca aggcttgagg agaacagcaa atcgccgggt
    58801 ttgagatcag gtgccgttat ttcttttatt gattgttcgg tgaaagagct ttgatgctgg
    58861 aatttgacag cccatgtttt tgcctcggca tctactgctg tggctgatgg gtctggctgg
    58921 ctgatatcaa cggtacaagc cgaaagtaaa agaaagctgg ggagaagcag gcggcagtac
    58981 gcctttggtt tatccatttt atacaatcca tgtaaaaaag ggccctgaaa ttcaggaccc
    59041 tttctggcat cagcctttaa tctgtttcac cagatattcg acgatgtcac cagtcttaat
    59101 taactgtttc tcgccgttgc gacgatattt atattcgata tcgtcgttgt cgaggttacg
    59161 gtcgcccagc acaatagtgt gcggaatacc gatcagttcc atatcagcaa acatcacgcc
    59221 cggacgctct ttgcggtcat ccagcagcac ttcgatacct tgtgcacgca gttcgctgta
    59281 cagtttctca gcaagctctt gtacgcggaa ggatttgtgc atgttcatcg gcagaatcgc
    59341 cacctggaac ggcgcgatag cgtcaggcca tacgatgccg cgttcgtcgt agttctgctc
    59401 aatcgccgca gctaccacac gcgttacccc gataccgtag caacccatcg tcaggatttg
    59461 gttacggcca tcttcaccct gtacggaggc tttcagtgct tcggagtact tggtacccag
    59521 ctggaagatg tgaccaactt cgataccacg tttgatcagc agcgtaccct ggccatccgg
    59581 gcttggatcg ccagccacca cgttacggat atctgcaact tccggggtag cgacatcgcg
    59641 atcccagttg ataccgaagt agtgtttacc atcgatgtta gcaccagcag cgaaatcact
    59701 catcgccgca acggtacggt caatcaccac cggaatcggc atgtttaccg gacccagtga
    59761 acccggaccg gctttaacca cagcacgaat ttcttcttcg gtcgcgaaag tcagcgggct
    59821 tgcaacctgc ggcagttttt ctgctttaac ttcgttcagc tcgtgatcac cgcgcaccag
    59881 cagcgcaacc agcgggaagc tactgccttc aaccgcttta accagcagag tcttaaccgt
    59941 tttctcaatc ggcagattga actgttcaac cagttccgcg atggttttcg cgttcggcgt
    60001 atcaaccagc gtcatttcct gggtagcagc agcgcgcggt tctttcggcg cgatagcttc
    60061 tgccagttca atgttcgctg catagtcaga ggtgtcggag aagaccacat cgtcttcacc
    60121 gctctgcgcc agcacctgga attcgtgaga ggcgctgccg ccgatagaac cggtgtcggc
    60181 ctgtacggcg cggaaatcca gccccatgcg gctgaagatt ttgctgtagg ccgcatacat
    60241 tgcatcgtag gtttcctgca gggattcctg agaagtatgg aaagagtaag catctttcat
    60301 caggaattcg cgggaacgca tgacgccgaa acgcggacgc acttcgtcgc ggaacttggt
    60361 ctggatctga tagaagttca gcggcagctg tttgtaagag ctaagctcgt tacgaatcag
    60421 gtcagtgata acttcttcat gagttgggcc gagtacgaac ggacgctcgc cacggtcaac
    60481 aaaacgcagc agttccggac cgtactgttc ccaacgacca ctctcttgcc acaaatcggc
    60541 tggctgaacc accggcatcg acacctcgat cgcaccggcg ttgttcatct cttcacgcac
    60601 gatgttttcg acttttttca gaacgcgcac gccggtcggc agccaggtat ataacccgga
    60661 ggccagcttg cggatcatcc cggcgcgcag catcagctga tggctgatca cctcggcgtc
    60721 ggcaggtgtc tccttgagag tggagagcag gtattggcta gtacgcatgt tgttacggtt
    60781 ccagttggaa ggtagaacag gctcaaggcg agcctgggac aaaaaaagtg atttagttta
    60841 ccagtgcaaa agaaatgtca aaagagaagg gcgtgaattt aacgcggttc cagcgcaaag
    60901 acttcaaaac ctgcgtcggt gacgcgccag cgaacgttaa aatcatgtag ccagacggca
    60961 taggttttgc ccgtttcctc acctttacga taggccgggc gcgggtcctg cgccagtact
    61021 tcgcggataa acagcgttaa ctgcggataa cgcttctcca gcgtcaaaag ctgcttttcg
    61081 acctctgcgg taaaactcac cgccatctct gcagctggcg cactttgcgc atagctggca
    61141 ctggcatcgg gaagcgattc ggcaaagggg agatacggtt tgatatccac taccggcgta
    61201 ccatcgacca gatccagact gccgagcttc agaatcacgc tgcctttatg gcaaacaacc
    61261 tctttcagct ctaccagcga catgccaatt gggttagggc ggaaagtaga gcgtgttgcg
    61321 aaaaccccca ttctggcgtt accgccgagg cgcggtggac gcacagtcgg acgccagccg
    61381 ccttccatcg tttgatgaaa gacgaaaagg atccataaat ggctgaatgc ttccaggccg
    61441 cgaacggcgt cggcctggtt gtaggaagct atgagatgca gttcgccgtt ggcgcttttt
    61501 accagacctg gctggcgcgg aacggcgaat ttttctttat agggcgagcg aataacgcct
    61561 atttgctcga actggaaact gctcatttcg ccgtaatgtt aagcgcagag ccgatacata
    61621 cagcctgacg atagcagccc ggcgtaccgc tggtgacttc gcagctatgc agtaatacag
    61681 cattggcttt cattttagag gcattgattt gcatctgctt acgtgcggtt ggaatgctcg
    61741 gcggagagtc ctgattagag gcctggcaag agtcgccact gacttcaccg agatcgcgga
    61801 acggtttgcc gactaattct tctgcattgg tataaattcg gaccggcgtg gcgcgcggcg
    61861 ctttcggttt tgcaggctcc gctttcggct ggggtgcagt gctttgaacg ggttcgacag
    61921 gggatctgct taacatggaa cagccgctta gcatgagtgc tactaaacag atcggtaaag
    61981 cacgcatagt atttcctcaa tgtatgatca aaacgtcaat attgaatcag gagcttgtaa
    62041 aaatgacaag acgggcaagc gcccgtcctg aatgatatta caaattgtgg aaacagccta
    62101 aaaattacca gcctttaaca gcgccgccgt taaacacttt gtttgctgct tcgtaaactt
    62161 catcagactg ataagcctgg acgaatttct tcacgttctc ggcgtctttg ttatcttcac
    62221 gcgttacgat caggtttacg tatggggagt ctttatcttc gacaaagata ccgtctttcg
    62281 ccggagtcag gccaatctgg ctggcatagg tggtattgat aactgccaga gcgatttgcg
    62341 cgtcgtccag agaacgcggc agttgcggtg cttccagttc aacaattttc agattttttg
    62401 ggttctcaac aacatcaaga acggtcggca gcaggccaac gccatctttc agtttgatca
    62461 agcccacttt ttgcagcagc agcagtgaac gaccaagatt agttggatcg tttggcacgg
    62521 caacctgcga accatcctgc agttcatcca gtgatttgat tttcttggag taaccagcaa
    62581 tcggataaac aaaagtgttg cctactgcga ccagtttgta gccacgatct ttcagttgct
    62641 gatcaaggta cggtttatgc tggaaggcgt tggcgtcgat atcgcctttg ctcaatgctt
    62701 cgtttggcag aacatagtcg ttgaaggtta ccagctcaac gtccaggcca tatttgtctt
    62761 tcgcaacttt ctgcgcgact tctgctacct gctgttcggc accaacaatc acgccgactt
    62821 taatgtggtt tggatctttt tcatcctgac cgcagcctac cagtgccagt gatccaatca
    62881 gggctcccac tgccgcaaag gttttgaatt tgaacgccat accttattcc ttcttcaatt
    62941 atttatgttg tgttgaacgt tacttgcgag tgacagcccg gacgatgcgg tcgcctgcaa
    63001 actgaattaa ataaaccaga atgaccagca ataccagtac cgtattcatc accgtcgcgt
    63061 tgtagccgat gtagccatac tgatagccaa tctgacctaa accaccggca ccgactgcac
    63121 cacccatcgc ggaataaccg accagggtaa tcagggtgat agttgccgca ttcaccagac
    63181 ccggcaacgc ttccggtaac agcactttac agacgatctg cattggcgtg gcacccattg
    63241 cgcgggaagc ttcaattaac ccggttggga tctccagcag agcgttctcg accatacggg
    63301 caataaacgg tgctgcacca acggttaacg gaacaattgc tgcctgcaaa ccaatcgatg
    63361 taccgacaat aacgcgggta aacggaatca tccataccag caagataatg aacgggatgg
    63421 aacggaaaat gttcacaatc gcagaaacgg tacgatacag cttcgcgtta gcaataattt
    63481 gccccggacg cgtgacataa agcagaacgc caaccggcag accaatcaca aacccaaaaa
    63541 atccggatac gaaggtcatt gccagcgttt cccatacgcc acgaaccagc agccacatca
    63601 tcggctcaga cataacccag tacctctact tttacatggt gttcctgcag ccaggcaatg
    63661 gcggcttgcg tatcttgttg tgtgccgtgc atttcagtca gcatgatgcc gaatttaacg
    63721 ccaccggcgt agtccatctg cgcgctaata atgttgttgt tgacgttgaa acgacgcgcg
    63781 gtttcagaaa gcagtggggc atcgaccgat tgaccggtaa actccagacg cagcatcggc
    63841 acgcagtcag taaatggctc cgtttgcaga cgttcctggt aatcttccgg gatatccaga
    63901 tgcagggtcg actgaataaa cttctgcgcc agcggcgttt tcggatgcga gaacacttca
    63961 cttaccgtgt cctgctcgat cagttcgcca ttgctgatga ccgccacgca atcacaaata
    64021 cgcttcacaa cgtccatttc gtgggtgatc aacagaatcg tcaaacccag acggcggtta
    64081 atgtctttca gcagttcgag aatagaacgt gtcgttgccg ggtccagcgc gctggtggct
    64141 tcatcacaca gcaatacttt gggattgctg gctaatgcac gggcaatcgc cacacgttgt
    64201 ttctgcccac cggaaagatt cgacgggtag ctatcatgct tatcgccaag accaaccaat
    64261 gacagcaatt ccgtcacgcg acgtttgatc tcgtctttcg gtgtgttgtc cagctccagc
    64321 ggcagagcca cgttgccaaa aacagtacgc gaagagagca ggttaaaatg ctggaaaatc
    64381 ataccaatct ggcggcgagc tttggtcaac tcggattctg acagcgtggt caattcctgg
    64441 ccatcgacca gcacgctacc ctcggttggg cgctccagca agtttacaca acgtataagc
    64501 gtactcttac ccgcgcctga ggcaccgata acgccataaa tttgtccagc tggcacatgc
    64561 aggctgacgt tgttcaacgc ctggatggtg cgggtgccct ggtggaacac tttggtgata
    64621 ttcgaaagtt ttatcattga ttatttatta tcgtcattaa gttagtcgta gcatctcgaa
    64681 tgcctgaaac gggcaacgcc gtcaatgaaa tggatgttaa ggcatccaga cgtctaaatc
    64741 aatcaggttt atgcgaagag cactttcttg caggtcgaaa catgcgatac tagcgtccca
    64801 tgccttatta aggagctata aaaggtggcg aagagcgtac ccgcaatttt tcttgaccgt
    64861 gatggcacca ttaatgtcga tcacggctat gtccatgaga tcgacaactt tgaatttatc
    64921 gacggtgtta ttgacgccat gcgcgagcta aaaaaaatgg gctttgcgct ggtggtagta
    64981 accaaccagt ctggcattgc tcgcggtaaa tttaccgaag cacagtttga aacgctgacc
    65041 gagtggatgg actggtcgct ggcggaccga gatgtcgatc tggatggtat ctattattgc
    65101 ccgcatcatc cgcaggggag tgttgaagag tttcgccagg tctgcgattg ccgcaaacca
    65161 catccgggga tgcttttgtc agcacgcgat tatttgcata ttgatatggc cgcttcttat
    65221 atggtgggcg ataaattaga agatatgcag gcagcggttg cggcgaacgt gggaacaaaa
    65281 gtgctggtgc gtacgggtaa acctattaca cctgaagcag aaaacgcggc agattgggtg
    65341 ttaaatagcc tggcagacct gccgcaagcg ataaaaaagc agcaaaaacc ggcgcaatga
    65401 ttaaaagatg aacggttgaa ataaaaatgc atttttccgc ttgtcttcct gagccgactc
    65461 cctataatgc gcctccatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc
    65521 ggttgaagag aaaaaatcct gaaattcagg gttgactctg aaataggaaa gtgtaatata
    65581 cgccacctcg cgacagtgag ctgaaagctg cgtcgcaact gctctttaac aatttatcag
    65641 acaatctgtg tgggcactcg aagatacgga ttcttaacgt cgcaagacga taaatgaata
    65701 ccaagtctca agagtgaaca cgtaattcat tacgaagttt aattctttga gcatcaaact
    65761 tttaaattga agagtttgat catggctcag attgaacgct ggcggcaggc ctaacacatg
    65821 caagtcgaac ggtaacagga agcgcttgct gcttcgctga cgagtggcgg acgggtgagt
    65881 aatgtctggg aaactgcctg atggaggggg ataactactg gaaacggtag ctaataccgc
    65941 ataacgtcgc aagaccaaag agggggacct tcgggcctct tgccatcgga tgtgcccaga
    66001 tgggattagc ttgttggtgg ggtaacggct cacc
//
LOCUS       DAETQL010000010        65872 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.010, whole genome shotgun sequence.
ACCESSION   DAETQL010000010 DAETQL010000000
VERSION     DAETQL010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.010"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(141..2588)
                     /gene="glgP"
                     /locus_tag="K0W65_000679"
     CDS             complement(141..2588)
                     /gene="glgP"
                     /locus_tag="K0W65_000679"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBI3094070.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2607..4040)
                     /gene="glgA"
                     /locus_tag="K0W65_000680"
     CDS             complement(2607..4040)
                     /gene="glgA"
                     /locus_tag="K0W65_000680"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBI3094071.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(4040..5335)
                     /gene="glgC"
                     /locus_tag="K0W65_000681"
     CDS             complement(4040..5335)
                     /gene="glgC"
                     /locus_tag="K0W65_000681"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBI3094072.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5353..7326)
                     /gene="glgX"
                     /locus_tag="K0W65_000682"
     CDS             complement(5353..7326)
                     /gene="glgX"
                     /locus_tag="K0W65_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBI3094073.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7323..9509)
                     /gene="glgB"
                     /locus_tag="K0W65_000683"
     CDS             complement(7323..9509)
                     /gene="glgB"
                     /locus_tag="K0W65_000683"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBI3094074.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9782..10885)
                     /gene="asd"
                     /locus_tag="K0W65_000684"
     CDS             complement(9782..10885)
                     /gene="asd"
                     /locus_tag="K0W65_000684"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBI3094075.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            11077..11670
                     /gene="yhgN"
                     /locus_tag="K0W65_000685"
     CDS             11077..11670
                     /gene="yhgN"
                     /locus_tag="K0W65_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBI3094076.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11727..13067)
                     /gene="gntU"
                     /locus_tag="K0W65_000686"
     CDS             complement(11727..13067)
                     /gene="gntU"
                     /locus_tag="K0W65_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBI3094077.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(13071..13598)
                     /gene="gntK"
                     /locus_tag="K0W65_000687"
     CDS             complement(13071..13598)
                     /gene="gntK"
                     /locus_tag="K0W65_000687"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBI3094078.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13737..14732)
                     /gene="gntR"
                     /locus_tag="K0W65_000688"
     CDS             complement(13737..14732)
                     /gene="gntR"
                     /locus_tag="K0W65_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HBI3094079.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14956..15651)
                     /gene="yhhW"
                     /locus_tag="K0W65_000689"
     CDS             complement(14956..15651)
                     /gene="yhhW"
                     /locus_tag="K0W65_000689"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HBI3094080.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15774..16811)
                     /locus_tag="K0W65_000690"
     CDS             complement(15774..16811)
                     /locus_tag="K0W65_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBI3094081.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17144..17632
                     /gene="yhhY"
                     /locus_tag="K0W65_000691"
     CDS             17144..17632
                     /gene="yhhY"
                     /locus_tag="K0W65_000691"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBI3094082.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17691..19433)
                     /gene="ggt"
                     /locus_tag="K0W65_000692"
     CDS             complement(17691..19433)
                     /gene="ggt"
                     /locus_tag="K0W65_000692"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBI3094083.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19553..19993
                     /locus_tag="K0W65_000693"
     CDS             19553..19993
                     /locus_tag="K0W65_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBI3094084.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19980..20723)
                     /gene="ugpQ"
                     /locus_tag="K0W65_000694"
     CDS             complement(19980..20723)
                     /gene="ugpQ"
                     /locus_tag="K0W65_000694"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBI3094085.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20720..21710)
                     /locus_tag="K0W65_000695"
                     /pseudo
     CDS             complement(20720..21710)
                     /locus_tag="K0W65_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21712..22557)
                     /gene="ugpE"
                     /locus_tag="K0W65_000696"
     CDS             complement(21712..22557)
                     /gene="ugpE"
                     /locus_tag="K0W65_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBI3094086.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22554..23441)
                     /gene="ugpA"
                     /locus_tag="K0W65_000697"
     CDS             complement(22554..23441)
                     /gene="ugpA"
                     /locus_tag="K0W65_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBI3094087.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23539..24855)
                     /gene="ugpB"
                     /locus_tag="K0W65_000698"
     CDS             complement(23539..24855)
                     /gene="ugpB"
                     /locus_tag="K0W65_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HBI3094088.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25252..25965)
                     /gene="livF"
                     /locus_tag="K0W65_000699"
     CDS             complement(25252..25965)
                     /gene="livF"
                     /locus_tag="K0W65_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBI3094089.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25967..26734)
                     /gene="livG"
                     /locus_tag="K0W65_000700"
     CDS             complement(25967..26734)
                     /gene="livG"
                     /locus_tag="K0W65_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBI3094090.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26731..28008)
                     /gene="livM"
                     /locus_tag="K0W65_000701"
     CDS             complement(26731..28008)
                     /gene="livM"
                     /locus_tag="K0W65_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HBI3094091.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRTQLKLKNGAAKGEQA"
     gene            complement(28005..28931)
                     /gene="livH"
                     /locus_tag="K0W65_000702"
     CDS             complement(28005..28931)
                     /gene="livH"
                     /locus_tag="K0W65_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBI3094092.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28979..30088)
                     /gene="livK"
                     /locus_tag="K0W65_000703"
     CDS             complement(28979..30088)
                     /gene="livK"
                     /locus_tag="K0W65_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBI3094093.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30512..30895
                     /gene="panM"
                     /locus_tag="K0W65_000704"
     CDS             30512..30895
                     /gene="panM"
                     /locus_tag="K0W65_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBI3094094.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(31094..32197)
                     /gene="livJ"
                     /locus_tag="K0W65_000705"
     CDS             complement(31094..32197)
                     /gene="livJ"
                     /locus_tag="K0W65_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HBI3094095.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32468..33322)
                     /gene="rpoH"
                     /locus_tag="K0W65_000706"
     CDS             complement(32468..33322)
                     /gene="rpoH"
                     /locus_tag="K0W65_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBI3094096.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33567..34625)
                     /gene="ftsX"
                     /locus_tag="K0W65_000707"
     CDS             complement(33567..34625)
                     /gene="ftsX"
                     /locus_tag="K0W65_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBI3094097.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVTQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34618..35286)
                     /gene="ftsE"
                     /locus_tag="K0W65_000708"
     CDS             complement(34618..35286)
                     /gene="ftsE"
                     /locus_tag="K0W65_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBI3094098.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35289..36785)
                     /gene="ftsY"
                     /locus_tag="K0W65_000709"
     CDS             complement(35289..36785)
                     /gene="ftsY"
                     /locus_tag="K0W65_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBI3094099.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36935..37531
                     /gene="rsmD"
                     /locus_tag="K0W65_000710"
     CDS             36935..37531
                     /gene="rsmD"
                     /locus_tag="K0W65_000710"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBI3094100.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37521..37790
                     /locus_tag="K0W65_000711"
     CDS             37521..37790
                     /locus_tag="K0W65_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBI3094101.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37793..38152)
                     /locus_tag="K0W65_000712"
     CDS             complement(37793..38152)
                     /locus_tag="K0W65_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBI3094102.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38293..38919
                     /locus_tag="K0W65_000713"
     CDS             38293..38919
                     /locus_tag="K0W65_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBI3094103.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38993..41191
                     /gene="zntA"
                     /locus_tag="K0W65_000714"
     CDS             38993..41191
                     /gene="zntA"
                     /locus_tag="K0W65_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBI3094104.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41293..41538)
                     /gene="tusA"
                     /locus_tag="K0W65_000715"
     CDS             complement(41293..41538)
                     /gene="tusA"
                     /locus_tag="K0W65_000715"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBI3094105.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41759..42424
                     /locus_tag="K0W65_000716"
     CDS             41759..42424
                     /locus_tag="K0W65_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBI3094106.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42497..43054
                     /gene="dcrB"
                     /locus_tag="K0W65_000717"
     CDS             42497..43054
                     /gene="dcrB"
                     /locus_tag="K0W65_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBI3094107.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(43058..44275)
                     /locus_tag="K0W65_000718"
     CDS             complement(43058..44275)
                     /locus_tag="K0W65_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3094108.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44409..45458
                     /locus_tag="K0W65_000719"
     CDS             44409..45458
                     /locus_tag="K0W65_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBI3094109.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45513..46100
                     /gene="acpT"
                     /locus_tag="K0W65_000720"
     CDS             45513..46100
                     /gene="acpT"
                     /locus_tag="K0W65_000720"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBI3094110.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46211..47785
                     /gene="nikA"
                     /locus_tag="K0W65_000721"
     CDS             46211..47785
                     /gene="nikA"
                     /locus_tag="K0W65_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBI3094111.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47785..48729
                     /gene="nikB"
                     /locus_tag="K0W65_000722"
     CDS             47785..48729
                     /gene="nikB"
                     /locus_tag="K0W65_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBI3094112.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48726..49559
                     /gene="nikC"
                     /locus_tag="K0W65_000723"
     CDS             48726..49559
                     /gene="nikC"
                     /locus_tag="K0W65_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBI3094113.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49559..50323
                     /gene="nikD"
                     /locus_tag="K0W65_000724"
     CDS             49559..50323
                     /gene="nikD"
                     /locus_tag="K0W65_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBI3094114.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50320..51126
                     /gene="nikE"
                     /locus_tag="K0W65_000725"
     CDS             50320..51126
                     /gene="nikE"
                     /locus_tag="K0W65_000725"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBI3094115.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51132..51533
                     /gene="nikR"
                     /locus_tag="K0W65_000726"
     CDS             51132..51533
                     /gene="nikR"
                     /locus_tag="K0W65_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBI3094116.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51653..52012)
                     /locus_tag="K0W65_000727"
     CDS             complement(51653..52012)
                     /locus_tag="K0W65_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBI3094117.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(52009..52284)
                     /locus_tag="K0W65_000728"
     CDS             complement(52009..52284)
                     /locus_tag="K0W65_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HBI3094118.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52343..53467)
                     /locus_tag="K0W65_000729"
     CDS             complement(52343..53467)
                     /locus_tag="K0W65_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3094119.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53467..56202)
                     /gene="rbbA"
                     /locus_tag="K0W65_000730"
     CDS             complement(53467..56202)
                     /gene="rbbA"
                     /locus_tag="K0W65_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBI3094120.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56199..57266)
                     /locus_tag="K0W65_000731"
     CDS             complement(56199..57266)
                     /locus_tag="K0W65_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBI3094121.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57632..59254)
                     /locus_tag="K0W65_000732"
     CDS             complement(57632..59254)
                     /locus_tag="K0W65_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBI3094122.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59516..61105)
                     /locus_tag="K0W65_000733"
     CDS             complement(59516..61105)
                     /locus_tag="K0W65_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBI3094123.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61490..62521
                     /locus_tag="K0W65_000734"
                     /pseudo
     CDS             61490..62521
                     /locus_tag="K0W65_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62608..63084)
                     /locus_tag="K0W65_000735"
     CDS             complement(62608..63084)
                     /locus_tag="K0W65_000735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094124.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(63077..63841)
                     /locus_tag="K0W65_000736"
     CDS             complement(63077..63841)
                     /locus_tag="K0W65_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBI3094125.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            64087..64848
                     /locus_tag="K0W65_000737"
     CDS             64087..64848
                     /locus_tag="K0W65_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI3094126.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64955..65455)
                     /locus_tag="K0W65_000738"
     CDS             complement(64955..65455)
                     /locus_tag="K0W65_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094127.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65606..>65872)
                     /locus_tag="K0W65_000739"
     CDS             complement(65606..>65872)
                     /locus_tag="K0W65_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI3094128.1"
                     /translation="SGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGIAYV
                     SGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16010 a        17690 c        16204 g        15968 t
ORIGIN      
        1 tcaagagcct gtccaatgtg cataaaaaaa tccggaaaca agtgagcgtt tccggattct
       61 tacacagcca ctggatcggt caactgatcc ttaactgatc ggcattagac cctttggccc
      121 cgttctattt attagtgaaa ttacaatctc accggatcga tatgccagat atgatcagcg
      181 tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt cagcatcgct
      241 tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg acaatcgaca
      301 tagctgcgat aatccgccag tacctggtag tgatcgccga agttgatcag cgaatcaacc
      361 agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat ttgcgtcagc
      421 acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc ctgacgacgc
      481 agttcttcca cttcttccgc tgtgttacca aaaataaaga tattgtcagc accgacatga
      541 tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc gttaagcgca
      601 aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg ttcagacaga
      661 tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat gaacacgact
      721 ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc attgatcaaa
      781 tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa aatattcacg
      841 cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt aatcacatgc
      901 aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac atcgaacaac
      961 gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag acgctttttg
     1021 ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca gtgttgttgc
     1081 agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc gtccagcacg
     1141 gctgaaagcg atgggttcgc taccgccagc cagcgacgcg gcgtcacacc gttggtgacg
     1201 ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg caccatcaga
     1261 ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc cagccaggcc
     1321 atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag cagatcggta
     1381 tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc aaagatgatc
     1441 tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc cagcgcctcg
     1501 ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa cgcgtcgtcc
     1561 cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga cagtaccgga
     1621 tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt tttatgcaac
     1681 tgataatgac ggcttaaaat gtcctgaatg gtcgaggaaa ccaagaagta ttcctgacgc
     1741 aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg agatacgttc
     1801 tcggagtggt ttttatcttc cactgccgcg aagtagtcac cctggttgaa tttaccgagg
     1861 ttaatttcgc tactggcttg cgcactccac aaacgcagcg tgttggtcgc gtcggtgtcg
     1921 taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc aatccagcgc
     1981 gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg cgtgttgtgg
     2041 cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc tttctggcta
     2101 ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta accgcgcccc
     2161 ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc caggccaccg
     2221 ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa ccccatcgct
     2281 tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt ggagagcgta
     2341 cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg cgacaactgg
     2401 gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa taacgttgcg
     2461 ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa catcagcttg
     2521 taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata tgtaaacgga
     2581 gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc acggtacgac
     2641 ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac aaaccgccac
     2701 agtgaaggac gggaccacag tacaaaagca cgccgaatag cccgtaacag cgaccaggca
     2761 ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc aagagaacag
     2821 tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt accgtacttc
     2881 aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag aatgacgtcc
     2941 gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac gcccacctga
     3001 ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc gcccgcgccg
     3061 agtagcgcca gctgcccgcc ctgctccaga agccccggta aggcttccag caccaggtcg
     3121 agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt atcgtcaacc
     3181 ttaagcccca ttgcgatttg taactggctc ttattttccg ctttatcttc caacgtatcg
     3241 cgggtgtaac gcgaggccaa cagtaagtcc gtctctggac tccagatttt ctcgtccacg
     3301 ccgttcagta cgcctgaaag acgcccttca cggtgacgct gttgcaacag accttccata
     3361 ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac cgccgtaatg
     3421 tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa ttccagccca
     3481 tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc ataaaacatg
     3541 ccttgatagg ccaggttgtg cacagtaaac accgacttcg caggacgccc gcgcgccgcc
     3601 agatacgcag gcgcaagacc tgcatgccag tcgtgcacat gcaccacatc aggacgccag
     3661 aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagcagcgc aaaacgcaat
     3721 acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg acgatcatag
     3781 agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa caacagcgtg
     3841 atatgcccgg cgaaagtatc acgacgggat actacctgcg catcggtcac gccacggcga
     3901 atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat ttgtgctgcg
     3961 ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa catctctgaa
     4021 catacatgta aaacctgcat tatcgctcct gtttatgccc taacttccgt agcatttcgc
     4081 gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct tcctctgcgt
     4141 tttcaccaat caccatgcct tccgggataa cacaagcacg atcgatgacg cagcggcgca
     4201 gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca atgttgcaga
     4261 atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa ccggagatca
     4321 cacaaccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag cgatcctgca
     4381 cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg cgatcgtaca
     4441 tatccagttc cggcaccaca gaggccagat cgagattcgc tttccagtaa gcttccagcg
     4501 tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa gagagcggga
     4561 acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct ttgccaaagt
     4621 cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga tagtcggcgt
     4681 caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc attgacggtg
     4741 ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc gccataacgc
     4801 caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa cgtgcgcctt
     4861 tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg tcgcccgcca
     4921 ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg gtgaccgcat
     4981 ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc agcagatcga
     5041 caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc tgcaccagag
     5101 tgtgggactg gtactgggtg atcacgccca tacgacggat cccagagttg atgcagttag
     5161 acagcgcaaa gtcaataatg cggaacttac cgccgaagtg tacggccggt tttgctcgct
     5221 tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg gcaacagatt
     5281 tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta accatgacta
     5341 actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct gccagacaac
     5401 cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact ccccagcagg
     5461 taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc gatccgagag
     5521 cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag gttgagcata
     5581 tcgatttagc caacggacat tgccgtcacc ttcttcccac cagcgattct ccaccaaagc
     5641 gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac cactgcttgc
     5701 ctgcgaccag tccaaccagt ttaattggtt atcctgacag taggcattgt tattgccatg
     5761 ctggctgtga ccatgttcgt caccggccag taacatcggc gtgccctggg agagcaacaa
     5821 cgttgttaac agggcatgaa tgctgtcgcg ccgccgttca accaggtcaa gagtaccgcc
     5881 taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc gattttcttc
     5941 tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg taaaaccgtc
     6001 atgcgcggtg acgagattaa tcgcggcact cggcagacga ccattacgtt taaaaacatc
     6061 gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat gtagccagaa
     6121 acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg ggaaatttcc
     6181 cacctgataa ccactaggag cgatatccca cggttcagca attaacttca cctgcgagag
     6241 caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact ctggcgtacg
     6301 gcccatgact gccgccagat caaagcggaa accatcgacg tggcaggttt ctacccaata
     6361 acgcaggcag gcgctggcat aatccaccac cgccggatga ctcaaattga gcgtgttgcc
     6421 gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc tacggttatc
     6481 gatcccacgc agcgagaata acgggccgtc gaggtccagt tccgcactat ggttgagcac
     6541 gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat cgcgaaactc
     6601 atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca tcgccaccgg
     6661 gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac tggcaaactg
     6721 cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt tgatcatcac
     6781 cggatgcccg agggctttat aagtgccacg gatctcgacc gggatctccg ggtgcaagta
     6841 cgttaatcct ttgacatggg cttcataaat gatggtgctg ccccacggcg tgcgcggcgg
     6901 ggcgtcatct tcccagtcat agtgatcaac caccactacg catttcggcg caatggcggc
     6961 gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat ctttaaactc
     7021 cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa agcgatgccc
     7081 ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg ggcgcgcatc
     7141 cggcagataa ccgtgccaaa tgtcgccact gttccctggc aagtcatagc gatgttcctg
     7201 gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga aaagtgtgaa
     7261 gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa tggcgagttg
     7321 tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagcgt caggcttagt
     7381 gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc attgcctgca
     7441 ttactgccgt gatagtgtat ggaatcggta ttgaggattt cacgccattt acctggctga
     7501 ttaatgccga agcgataatc atgacgcggc accggcgtaa agttactggc aacgataatt
     7561 tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt gtcatccacc
     7621 accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt atggtggcgg
     7681 taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt atcgccgcct
     7741 tccaacagat gccagtcgag gctggcgtca tggttccact cgcggccctg ggcaaattcg
     7801 ttccccatga acagcagttt cttgcccgga aatgcccaca tccagccata gtaagcgcgc
     7861 aggttagcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga ttttttaccg
     7921 tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt gtagagcatc
     7981 ccgaaggtca gtttatcgtg atgatactga cgataaatcg ggtcgagctt catgtagtcc
     8041 agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc gcccatatcc
     8101 tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac cgcaccggaa
     8161 acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc aagattctcg
     8221 cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc acgataaatc
     8281 atcgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat ccagtaaagc
     8341 gcgttaccga cgaggaagtt actgacttca cggcgaccat agttgtagat cagcgtgttc
     8401 cagtcctgat gatagccttc acgcggatcg ctgtgttcat acaagttcgt gccatcaaat
     8461 tcggcaagcg caaagtcatc agtcgggaag tggcctggca cccagtcgag aatcacgttc
     8521 agaccagctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt accaaaacgg
     8581 cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa gggatgctcg
     8641 ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg caccagttga
     8701 tcggccagct cgcggtagct caaccagaaa ttgttgtcgg tgtggcgacg ccaggaaccc
     8761 aggtgaactt cataaataga gattggcgca tcaaactgat tcgctttttt gcgctcttca
     8821 gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg gcgcatttgc
     8881 gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc aatcatctcg
     8941 tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccagat gccgctctct
     9001 ttacgcaggc gcatcgggtg acggcgaccg tcccagtagt tgaattgccc aaccaccgag
     9061 acccgacggg cgtttggagc ccagacagag aaacgcgtac ctgtgacgcc atccatagta
     9121 tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga taatagccag
     9181 gcatccattt cctggattag cggaccaaaa cggtaaggat catcaatcag gttttgctga
     9241 ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg aatgacgccg
     9301 ctaaagaatc cccgtgagtc gagacactcc agttttgcga gtttgcgccc ggttttcggt
     9361 tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag tcccgcggtg
     9421 gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat tagcgcgtta
     9481 atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg tgtcacccca
     9541 tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg cgcagcactc
     9601 tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc atgtattcag
     9661 ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga aacatctgcg
     9721 cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta atgaataaag
     9781 attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac agcagctggt
     9841 cgcccacggt aaaggctgac aggaactctg gtcccatatt cagcttacgc aggcggccta
     9901 ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata gtgatttccc
     9961 gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct tcgacggtcg
    10021 gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag cgcaatgccc
    10081 cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg atcttgttgg
    10141 tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt ttgtcgatcc
    10201 acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc tcaccgctac
    10261 gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg gtcgcgagtt
    10321 catctgccac atggccatac agatggccca tctgggttaa taactcacgc atatgtcgcg
    10381 caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca agatcattgg
    10441 cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg ccaacaaaag
    10501 tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg tcaagaatga
    10561 tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa ccttgccatc
    10621 cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga caggtcacaa
    10681 tgatatcgag ggcctttagc gcctctagat caaaggcatc ctgaagtgtg ccagtggttc
    10741 cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca gggcgaatgg
    10801 cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc ataccgcgcc
    10861 agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg tgctgtataa
    10921 atgtgccggt ctcctcttgg cacatctttc accatacaaa aagctgccaa agtcgcaagt
    10981 gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt tgttagcacg
    11041 cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat ttctgcagca
    11101 gttttattga tcctgattat ggatccgctc ggaaacctgc ctattttcat gtccgtactg
    11161 aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct tattgctctc
    11221 ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct cagcctgcga
    11281 gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat taagatgatt
    11341 ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc atttatcgtg
    11401 ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat gttgttgtct
    11461 catcagtacc cgaatcagat gggacatctg gtgattgctc tgctgctggc ctggggcggc
    11521 acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga gaaaggggtg
    11581 aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaactca gatgttcctc
    11641 gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa gagcccggta
    11701 gcgtcacaac taccgggcga acaaacttaa ctcaacagct ggaacgcaat catcccaacg
    11761 atggcaccaa cagtgccgag gatggtttcc atcatggtcc aggttttcag cgtttcggct
    11821 tcggtcgcgc cggtaaattt accgaacaac cagaaaccag cgtcgttaac gtggctgaca
    11881 acaatcgaac caccagcgat acaaatcgac agtgccgcca tttgcgcacc agagtagttc
    11941 agttgttcaa tgactggcat caccagtccc accgccgtta aacaggctac ggtggcagcc
    12001 ccctgaatga tgcgcactgc agcagccagc acgaagcagg tgatagcaat cggcaggccc
    12061 atgccggtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag cacctgtttg
    12121 aacacgccgc ctgcaccaat caccagcaga atgatccccg ccggttgcag cgcgtgaccg
    12181 caaatctcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc ataaatcgct
    12241 accagacaag caaccagaat cgcggtaaac ggatgaccaa taaactcgaa ccattcgtaa
    12301 gcggtagagc cttctggcac aaaacgcgcg gcaatggttt tcagccccac cagcaccagc
    12361 ggcaacagga tcagcgacag gctgaatccg aaagatggca ttttaccttc gccgagatgc
    12421 ggttcgctga tgtcgtcagg aatatgcagc tcaacgtaac ggctgatgaa gttaccccac
    12481 agcggcccgg caataatcat tcccggaatt gccgcacaca ggccaatcag gatcatccag
    12541 ccaaagtcgg cattcatctg cgatgccagc agcattggcg ctggcccagg caccaggaac
    12601 gctgccgcgg ccgccacgcc tgcgaataat gggattacca gcttcaccag gttcgtaccg
    12661 gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa caatcgccac ttcaaagaac
    12721 agcggtagcg cacagaccag ccccgcaagg ccgatggcat aatgcgcgcg gctgtgaccg
    12781 aaggatttga gcattttgac ggcaatctga tcgactgcgc cggtttcata taagatcttg
    12841 ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggtgcc tcccatccct
    12901 ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc agcccccatg
    12961 gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa aaacagcagc
    13021 agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac ttatttgcct
    13081 tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc gatatccacc
    13141 accagtacat cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg cgtcaccaac
    13201 atttgggttt taaagaaatg gcctttgcgc gctttcaggc gactttcaat cacatcaaaa
    13261 tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa gtcgcgatag
    13321 tgctttttca gtgcagaaca ggcgatcagc gacactttat tggtgcgctg catagcaaac
    13381 gcggagtcgt tcagcgcctg caaccagggt ttgcgatcgt cgtcattcag cggttcgcca
    13441 gacgccattt tttcgatatt gcgccgtgga tggaggaaat cgccatcaag aaacgcggca
    13501 tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcccga tacgcccatc
    13561 aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc acaatacaag
    13621 aattaaaaat gttacgggta actgttatcg gcaacattgt ccagccggac aatatcagaa
    13681 gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt gtgggcttaa
    13741 atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt cacagattcg
    13801 ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc acgcggcgtc
    13861 agcacgctcg caagtcgtgg ctccatcacc tgaccaatgt catgaccgtg gaaaccggca
    13921 atcgccatat cgtcaggaac ttttaacccc agacgctgac attcaaacgc cgcgccgacc
    13981 gccagatcat cattagtaca gaacacgcca tccagctgcg gatattcccg ccgcgcctgg
    14041 cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac gctatatggc
    14101 accaggcctg catccagcat cgcctgttcg tatcccttct gtttgatgat agtacgttcg
    14161 tcgagacgtg cgccgagata ggcaatgtgg cgatgcccac gagcaataat ggcggtagtc
    14221 atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca cggcgactgg
    14281 ctgtccatca actccaccac cgggatcccc gccacttcaa tcatctttaa ggtgcgcggc
    14341 gtgtgggtac gttcggtgag gatcaggccg tcgatattcc aggagagcat ggattcgaga
    14401 cgttcttgct ccatttccgg tttataaccg tagtgcgcca gcatggtctg ataaccgtgc
    14461 gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt ggtgagagaa
    14521 ggtaacagga cgccaatcgc ccggctggta gcgttagaga ggatatcggg cgcacgattg
    14581 ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc gacggaaacc
    14641 tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac acggtcagcc
    14701 acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa gagatagatt
    14761 ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct tgtttacaat
    14821 tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc aaaaccccgg
    14881 aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg caggcttcaa
    14941 agatgccgtt gagttttaaa ccggcggcag atcgaacagt aacacttcgc tgtcgctatc
    15001 cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg tcgaggcttt
    15061 cacgccgtta atggtaacat tgccttttac cacctgaatc cagacgcggc gttcagcggc
    15121 aatctgatgc accgactgct catctttcag caacgcccaa cgatacagtt ccatatcctg
    15181 atgcactttc agcgaaccat ctcgcgcatc cggcgagagc accagctgtt tgccctgcac
    15241 ggcatcgaag cgacgctgtt cataacgcgg cgtaatgcca ttttcttcag gcatgatcca
    15301 gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt gacgaatacc
    15361 cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat tgcccatgct
    15421 gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat ctttatgcgg
    15481 gtgagtgccg aagccctgcc ctacttcaat cacgtcgtcg ttaatcacgc gcagcgcgga
    15541 gaagcccata aagttcggat cgtaatagtt ggcacaagag aaagtatgcc aggagtccag
    15601 ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca tttgtattcg
    15661 ccccctgaat gatttcgatg ggcttagtgt ggacccgatc tgcctgggat gatagagggt
    15721 gaaaattgcc ccctctgttc aaaaaaattg aactattcga ggggccatca aacttaattc
    15781 gcgagagtca ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat ttccagattg
    15841 gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt ttgatacaac
    15901 gcatcataaa cacgcccgta atcgcccatc tccggcttca tctcttctct gaccgtcaca
    15961 ccctcgtcat tgacatactc cagcacaccg accgaatcat ccgctgcgaa tcccggttcg
    16021 cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta tttaataaac
    16081 gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag atggctggtt
    16141 ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc gtcaggattg
    16201 gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc gaacagagaa
    16261 ataatctggt ccatcgtatg cacaccaagg ccatagaacg cgccatcctg cggcagtcca
    16321 ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc tttccacttc gacaatctct
    16381 cccagcttgc cgctttcaat cgcttttttc gccgtcagga agcaggaatc aaagcgacga
    16441 ttctgatatg gcgtgacggt cagccctttg cttttcgcca gtgcaaacag ctctttcgcc
    16501 tgcgcaagtg tcggagtgaa cgttttttcg accagcacat ttttcccggc ttccagcgcg
    16561 cgcttcgcgt actcgaagtg gctgtccgca tgggtgcaga caacaaccaa cttaacatcg
    16621 ggatcgttta gcacttcgtc gagatcgctg gtgaaatgga tatgagagta aatgggagcc
    16681 tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct atccttgcgg
    16741 ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat aaaggcgcag
    16801 ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg caaagcggac
    16861 gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa attgagaacg
    16921 aaagatcaaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc aatgtgagca
    16981 atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgatacaat aataatcatt
    17041 ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc atttgaaagt
    17101 caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg agatagtaat
    17161 acgccacgca gaaacacggg attacgaggc catcaggcag attcacgccc agccggaggt
    17221 gtattgcaac acactacagg tgcctcatcc ttccgatcat atgtggcagg agcgactcgc
    17281 cgatcgtccc ggcatcaagc aactcgtcgc ctgtattgat ggagacgtcg tgggccatct
    17341 caccattgac gtgcaacagc gcccacgccg cagtcatgtt gccgattttg gtatctgtgt
    17401 cgactctcgt tggaagaacc gcggcgtcgc cagcgccctg atgcgagaga tgattgaaat
    17461 gtgcgacaac tggttacggg tagatcgcat tgaactaacc gtgtttgtcg ataacgcgcc
    17521 agcaattaag gtctataaaa aattcggctt tgagattgaa gggaccggta agaagtacgc
    17581 attgcgtaat ggtgaatatg tcgatgcgta ttatatggcg cgggtgaaat agagaatttg
    17641 caatctaagt ttgtcattta agccctcacc taccgggagg gcttaagata ttaatacccc
    17701 gccgttaaat catccaccga gcgcgggtcg gatgcgccgt acaactcacc gtccggccca
    17761 accataatgc tttgtgtgct gcccatcgcc tctttcagcg ccactttctg accttttgct
    17821 tccagcagct tgagcgtatc cgggctaaac cctttttcga cacgcagctc gtccggcaac
    17881 cactgatggt ggaaacgcgg cgcattggtc gcttcggcga cgttcatgcc ataatcgatg
    17941 ctattcacca ccatttgcag caccgtggtg ataatccggc taccgcctgg gctaccggta
    18001 accagccagg ttttaccgtc tttcaccaca atggtcggcg acatcgacga cagcgggcgt
    18061 ttgttcggcc cgacggcgtt ggcatcaccg cccaccagcc cgtaaacgtt cggtacgccc
    18121 ggtttggcgg agaaatcatc catctggtta ttaagcagaa taccgctctc acccgcgaca
    18181 atacccgtac cgaaggtggt gttcagcgta taggtcaccg ccaccgcgtt accgtcttta
    18241 tccactacag agtaatgggt agtttgatta ctctcataag gcgcaagctt gccagggcga
    18301 atctcactgg acggcttcgc ttttttgata tcaatttgat cggcaatgga tttggcatag
    18361 gctttattgg tcagtgcctg ccacgggact ttgacaaaat ccgggtcgcc aagatattcc
    18421 gagcggtcgg cgtaggcgta tttctccgct tctgccatga tttgcatcgc atcggcgctg
    18481 ccaaagccgt atttcttcat atcgaagttt tccagaatat tgaggatttg tacgatatgg
    18541 atcccgccgg aggatggcgg tggcatggag taaacctgat acccgcgata atcgccgctt
    18601 atcggagtgc gttcgaccgc tttataggcc gctaaatctt ctttagtgat caagccaccg
    18661 tttttctgca tctcctgggc gatctgttcc gcaatcgtgc ctttatagaa ttcgtccggg
    18721 ccgttttcag caatcatctc caggctcttt gccaggttcg cctgcaccag cgtgtcgccc
    18781 tttttcagcg gctcgccctc tttccagaag atagctttac tgttttcgtg attcggcaac
    18841 acttcgctac cgtaggtttt gagatcgtca gccagcgcgt cgttaacgat aaaaccatcg
    18901 cgtgccagtt taaacgcggg ctgcacgact ttgttcagcg gcatggtgcc gtatttatcc
    18961 agcgccagcg agaaacctgc taccgtaccc ggtgtgccgg aagccagatg cgaagtgagt
    19021 gattttttgc tgtccgggtt gccctgatca tcgaggaaca tatcgcgggt cgctttggcg
    19081 ggtgccattt cgcggaaatc gatagccgtg gtattgccat ttttcgagcg gattaacata
    19141 aaaccaccac cgcccagatt ccctgcctgc ggatgcgtta ccgccagcgc gtagcccacc
    19201 gccacggcgg catcaacggc attcccgccc tccttgagga tatccacccc cacctgagtg
    19261 gcagtggcgt ccacagacgc taccattccc tgtttcgcgc gtaccggatg gaagacatct
    19321 tcctccacac catacgagac gggcggcgca ggaggcgcgg cggcggcgct aaaacaactt
    19381 cctgagagca gagcagcaat ggccacccgg cgtaaaaacg tcggttttat catcgttatt
    19441 ctccagagat taaggggcaa ccccagctaa gcctggtata taactctgaa ttaatcatcg
    19501 ttttgtcggg aagcgagtaa acttaaagga tatcttcaga ggaggatacg cgatgaaacg
    19561 acttctgatt cttacggcac tcctgccgtt tgtcggcttt gcacagccca ttaatactct
    19621 gaacaaccct aaccagccgg ggtatcagat acccagccag cagcggatgc aaacccagat
    19681 gcagactcag caaatccagc aaaaagggat gctgaatcag caactgaaaa cgcaaactca
    19741 gttgcaacag cagcatttag aaaaccagat aaacaataat tctcagcggg tgttgcagtc
    19801 acagccgggg gagcgaaatc ccgcccggca gcaaatgctg cccaacacca acggcgggat
    19861 gttaaacagc aaccgtaatc cggatagttc gttgaatcag cagcatatgc tgccggagag
    19921 gagaaacggc gacatgctga atcagcccag cacgccgcag cctgacattc cgttgaaaac
    19981 tattgggccg taaagttcgg accaatcacg tcaatcgcat cggtacagat gcaatccacg
    20041 ccccagcgca gcaactctgc cgcgtgctgg ggtttattga cggtataaac cagaatccgt
    20101 aatccggcgt ctttcaactg catcactcgc gctttatcga gtaacttatg attgagatga
    20161 atagagacgc agcccagccg cgcggtcagt tcgcgccagt cgtcgcgcca ctcatcaagc
    20221 aataaaccac gcggcagttc cggtgccgcc tgttgtgcag cttctaaagc atcaatctca
    20281 aacgatgaca gcagcggcgg cgtcataccg gcccacagtt ggcgtgccgc cagcgccacc
    20341 attttgcccg ttaatggtcc agtgccagtg gtgggtttga tttcgatatt cgccatcatc
    20401 ccgtgttcgc gacagcgttc cgccacctgc gaaagcaacg gcagcggctc acctttaaac
    20461 gctttgctgt accaactgcc cgcatccacg cgcagtaaat cctgccagtt cagttcaccc
    20521 gcgacgcccc agccgttgct ggtgcgttcg agattgtcgt catggagcag gaagatctcg
    20581 ccatctttcg ataacttcgc gtcaaattcg atcatcttat gaccgtattt tgccccgacg
    20641 tcgattgccg ccagggtgtt ttccggggcc agcttaccgc cgccacgatg agcgacgatg
    20701 cggggataag gccagttact catactcgtt gtcctgtttc accatcaaaa agatgcagct
    20761 gattttccgc cagatgcagc cacagcgtgc tgcctgccgt cgggcgctcc tgatgcgcca
    20821 gtcgcaccac cagcttctgc ttgcgagctt agcgcaatat gttccgggcg aatgccgagg
    20881 gtcattttac gcccggcata ctgacggtag ccaccgttta gcggtagcac gataccgccg
    20941 tccagttcga aatgcgtgcc ttcattattc atgcggcctg tcagcaggtt catcgccggg
    21001 ctgccgataa aactcgccac aaacaggctg gcgggctttt cgtagacttc caccggcgtg
    21061 ccaatctgtt cggcaacgcc gccgttcatc accattactc gctgggcgag cgtcatcgcc
    21121 tcaacctgat cgtgagtaac gtagagtgaa gtcgttttca ggcgacggtg cagctgttgc
    21181 agttcaagac gcatctgtac gcgcagcttg gcatcgaggt tagagagtgg ctcatcaaac
    21241 aagaacaccg ccggatcgcg cacaatcgcg cgtcccatcg ccacacgctg gcgctgaccg
    21301 ccggaaagct cgcgcgggcg acgtttgagc agaccgtcca gctccagaat gcgcgccgct
    21361 tctttaacgc gctcggctat ttgctgcttg cccatgccgc gaattttcag cccccacgcc
    21421 atgttttctt cgacgctcat atgcggataa agcgcgtaat tctggaacac catcgcaatc
    21481 ccgcgatctt tcggctccat ttcggtcacg cgctggtcgt tgatccagat atcgccttcg
    21541 gtcaccctct ccagcccggc aaccatacgc agcagcgtcg atttcccgca gccagacggc
    21601 ccgaccatca cgataaattc gccatccgcc acatcaaggg tcagcggttt aatgacctag
    21661 gttttgccat cccagctttt ggttactgcc tgtaatttca gtcctgccat cttatttctc
    21721 actatcgacc aggccgcgca cgaaggcacg ctgcatcact aaaacaatca ctactggtgg
    21781 gatcagggtt aacaacatcg ccgccatcac tgagttccat tccgtggtgc cttcgccagt
    21841 ggcgatcatc cctttgatcc ctgccacggt ggtgccgaga tccacgtcgg taataatcaa
    21901 caacggccac aaatactggt tccagccgta aataaaggtg atcacaaaca gcgccgccag
    21961 attagttttg gagagcggaa aaacgatgtc gcaaaagaag cgcattggtg acgcaccgtc
    22021 gatccgcgcc gcttccacca gttcatccgg cagcgtcata aagaactggc gaaacagaaa
    22081 cgtggcggtc gccgaggcca tcagcggcag cgttaaaccg gcgtagctgt cgagcatctt
    22141 caggttggcg atgacttcca ccgtcgggaa gatacgtact tcaaccggca gcatcagggt
    22201 gataaaaatc atccagaaga agaggttacg tagcggaaaa cgaaaccaga caatggcaaa
    22261 tgccgagagc atcgagacag taattttgcc gagcgtaatg ctgaacgcca tcacaaagct
    22321 gttaagcaac atccgccaga acggcgcgct atttgtgcct accccgttca cccagatgtt
    22381 gtggatgttt tccagcagat gcgtgccggg gatgagcgtc atcggcgcgg cgtagacggc
    22441 ctgtttatcc agcgtcgccg cgacaaacgc cacgtacagc gggaagagga tcaccgcgat
    22501 cccgaggatc agcatggtat ggctgaatat cgtcagccac ggacggttct caatcattgg
    22561 taacgcacct tgctttcaac atagcggaac tgcaccaccg tcagcacgat gacgaggaac
    22621 atcagcacca tcgactgtgc ggcagacgaa gccagatcca gtccggtaaa accttcgcgg
    22681 tagatcttat aaatcagcgt cgtggtggcc tgaaccggcc cgccggacgt ggcggcgtcg
    22741 atcaccggga aggtgtcgaa gaaggcatac accaggttca ctaccagcag gaagaaactc
    22801 accggggcga taagcggcag cgcaatctta aagaagcggc gaatcggccc tgcaccgtcg
    22861 atggctgcgg cttcgatcaa cgaacgggga atggattgca gcgcggcata gaagaacaga
    22921 aagttgtagc tgatttgctt ccacaccgag gcaaacacca ccagaaacat cgcctgaccg
    22981 ctattttgcg cgtggttcca gtcgtagccg aactcagcga gaaaatgggt gatcagcccg
    23041 cgaccggggt taaacaggaa gatccacaat acggcggcaa cggcgggagc cacggcatac
    23101 ggcagcaaca ttaaggtttg gtagaaacga ctgccgcgca cgatgtactc caccagcgcc
    23161 gcaaagaaca gcgataccag caaaccgctg acggtgacaa aggtgctgaa ttttatcgtc
    23221 gtccagaagg agtcgagata atagctgtca tgaaacagcg tgacgaagtt atccagaccg
    23281 acaaactggc tggagaaacc aaacggatcg acgctttgta gcgagtacca caacgcttcg
    23341 cccgcaggcc agataaaaaa gataacggtg atgatgagct gcggcgcgac cagcagataa
    23401 ggcagccagc gcgagcggaa caccggacgg gatgatgaca ttacggttaa ttcctgaact
    23461 gtgccggatg cgcttcgctt atccggccta cacgcgatgc caggtcagat aagcgaaacg
    23521 catccggcat tacactgatt aagacttcgt cgatttctca aagcggcgca gtaactggtt
    23581 cccacgctca actgcggtat ccagcgcctg ctgtggcgtc ttcttaccgg tccacacgct
    23641 ctcaagctct tcatccacaa tcacgcggat ctgcggcatg ttgcccagac gaagcccttt
    23701 ggtgaacggc aacggcggct tgttcagcat ctgacgcgtc gcggtatccg cacctgggtt
    23761 tttctcgtaa aagccctgct cacgggtcag gtcatacgcc gctttggtaa ttggcagata
    23821 accggttttc tgatgccact cagcagcgtt ttctggcttc gcgaggaagt cgagaaactt
    23881 cgccacgccg gtatacgttt ctttatcttt gccctgcatc acccacaggc tggctccgcc
    23941 gataatggcg ttttgtggcg catctttcgc atcggcgtcg taaggcatca tacctacgcc
    24001 gtagttaaat ttggcgtact cgcgaatgtt ggcaagagaa ccggaagagg cggtggtcat
    24061 cgcgcaatca ccgttataga acttctcggt ggattcatcc ttacgaccga cgtagctgaa
    24121 gtcgcccttc ttgttcatct cctcgagcat ggcgatgtgt ttcacctgct ccggcttgtt
    24181 gaactccagc accgcgtccg tgccgtcaaa gccgttgttt ttgctggcaa acggcagacc
    24241 gttccaggcg ctaaagtttt ccagttggat ccagccctgc cagccgctgg cgtagccgca
    24301 cttaataccg gaggctttca gttttgcgga atagtccgcc agatcctgcc aggttttcgg
    24361 cggctgttcc gggtctaacc ccgctttctt gaaggcgtct ttgttgtaat agagaacgga
    24421 agtcgagctg ttgaacggct gggagagtaa gtggccggtt ttgctgtcgg agtagtaacc
    24481 ggaaaccgtc ggcacaaact gcgactcatc gaactgaatc cctgcctctt taaacacgtc
    24541 atacaccggt ttaatggctt tcgacgccat catggtggcg gtgccaactt cataaacctg
    24601 caaaatagcc ggagcgttgc cggtacgaaa tacggcaatc cccgcgctta aattctgttc
    24661 gtagttgcct ttataggtcg gtacaatttt gtaatccgga ttttcggcgt taaaacgttg
    24721 ggccagagaa tccacctctt tacccagttc cccttccata gaatgccaga acggaatggt
    24781 cgtcactgcc tgtgcattcc ccattaacgc cagtccgagc gccagtgctg aagctgtata
    24841 atgtaacggt ttcatcgttt atctctcttg ttgtaccgaa tgcgcgaatt cacgcgtttt
    24901 atgctcgcgg ggtaacatga catgctcgaa ttacagaaaa ataacttttt tgttacattt
    24961 gtaagatagt aaggtgtcag aaagatgaca aggcggtgac ggcgtgggtg agggaaaatg
    25021 ggagatgggg cacggataag cggaaaaata gaaggtctga atcaaactct acagattact
    25081 catcgtttca tgccggatgc ggcgtaaacg ccttatccgg cctacaagat cgtgcaaatt
    25141 caatatattg caactcaccc agtaggcctg ataagcgcag cgcatcaggc aattttgcat
    25201 ttgtcacctg tctcaaagga gtcttttgac tccttatcaa tcaacgtgtt attacccgcc
    25261 taaatacgca cttctcactg cttcgttcgc cagcagcgcg tcgccggtat cggagagcac
    25321 cacatggccg ttttccagca cgtagccgcg atccgccagc tttagcgcct ggttggcgtt
    25381 ctgctcgacg aggaagatag tcatcccctg ctcgcgcagc tgctcgatgg tgtcgaaaat
    25441 ttgctggatg ataatcggcg caagaccgag cgatggctca tcaagtaaca acaaacgcgg
    25501 attgctcatc agcgcgcgac caatcgccag catctgctgt tcaccgccgg acatggtgcc
    25561 cgcccgctga acacggcgct catgcagacg tggaaacagt tcatacaccc actttatgcg
    25621 ctcctgaaac tggtcacgtt cggcaaagaa gccgcccatc gccaggttct cttccaccgt
    25681 catccgcgag aagacgcgac gcccttccgg gacaatcgct accgcttcgc gcatgatttt
    25741 cgccgtctgc cagtcggtaa tgtctttatc atcaaacaca attcgcccgc tggtggcgcg
    25801 cggatcgccg cataacgtgc cgagcaaggt ggttttcccc gccccgttcg cgccaatgag
    25861 agtgacaatc tcgccctgat tgatatgcag gctcacctca tgcagcgcct ggattttgcc
    25921 gtagtgggcg ctgactttgt caaaggacaa catgactttt tccatcttat gcctcaccta
    25981 aataggcacg gatcacgtcc gggttattac ggatttgctc cggcgtaccg tttgccagtg
    26041 gcgtcccctg attgaccaca taaattcggt ctgaaatccc catcaccagc ttcatatcgt
    26101 gttcaatcaa caaaatagtg gtgttgtgat gattgcgcag ttcggcaatc agctcatcca
    26161 gctctttggt ttcttttggg ttaagacctg ccgccggttc gtcgagcatt aaaatctccg
    26221 gctgcgtcac catgcagcgg gcaatctcca gacggcgttg gtcaccatag gccaggttac
    26281 tcgcctgacg gttggcgtgt tccagcaaac caatgcgctc aagccaggtg gcggcgcggt
    26341 cgagcgcttc gctctgggcg cgacggaaag aaggcgtttt caacaggcca gagaacagcc
    26401 cggttttcag ttgctgatgc tgcgccacca gcaggttttc aattaccgtc atttcacgga
    26461 acagacgcac atgctggaag gtgcgcacca cgcccatgcg agcaatttgc tgccccggta
    26521 aaccttcaag atgctgatcg cgcagtaaaa tggtgccgcc ggtgggtttg tagaaaccgg
    26581 tcaggcagtt aaaaaccgtg gtttttccgg caccgttagg gccgattaac gagacgattt
    26641 cctgcgggta cagttcaaga ttgacgttgt tcaccgccag caggccgccg aagcgcatca
    26701 tcagaccgtt aacagataat aatggctgac tcatgcctgc tctcctttcg ctgcgccgtt
    26761 tttcagcttc agttgcgtgc gcgtcatggg cagcaaaccc tgcggacgcc agatcatcat
    26821 cagcaccatc aaaccaccga gcattaacat gctgtattcg ttgaaatcac gcatcaattc
    26881 gcgcgacacc accagcagaa cagccgccag aatcactgca aattgcgagc ccataccgcc
    26941 gagcaccact atcgccagca caaaggccga ttcagcaaag gtgaaggatt ccgggctgac
    27001 aaagccctga cgcgccgcaa acagcgttcc ggcaaaaccg gcaaatgcgg cgctgatggt
    27061 aaaagcagtc agcttgatac ggcgcgggct taagcccagc gaacgacagg cgatctcgtc
    27121 ttcacgcaac gcttcccacg cgcggcccag cggcatccgc agcaggcggt taatgacaaa
    27181 caggcttaac accaccagca gcaacgccac caggtagagg aagataacgc gatcggacgg
    27241 atcgtatttc aggccaaaga aattactgaa cgtgtcccag ccgccttcac gagcggtacg
    27301 gctgaactcg agtccgaaga gtgtcggttt cgggatctga ctgattccgt tcgggccgcc
    27361 ggtgatttca gtgttattga gcagcaatat gcgcacaatt tcgccgaaac caagggtaac
    27421 gatcgccaga tagtcaccgc gcagacgcag caccggaaaa ccgagcagga aacccgccgc
    27481 cgctgccatc agtccggcaa tcggcaaaca ggtccagaac cccagaccgt aatagtgatt
    27541 gagcagcgca aaagtgtaag cgccgatggc gtaaaaaccg ccgtacccca gcaccagcag
    27601 accagaaaga ccaacaacca cattcagccc gagaccgagg ataatgtaga tcatggtcag
    27661 agtggcgata tccaccgtcc cacgtgacac cataaacggc cacgccacca caagcaccag
    27721 cagcgccacg aggaacagct tttgcttcac cgtggaccca tcaatcgcgg gcagaataaa
    27781 cttcggcccg gaaacgcttt tcaacccttt ctggaaagcc ggtcgcaaaa gctggaagaa
    27841 aaagaccacc gccgtgccga taaacatcca ctgccaacgg atgtctgaag ccgtgtcgac
    27901 caccagtttg gtgccatcca gctccagttg tacgcccata aagacgcccg ccaggacaaa
    27961 gaacatcgcg gcagagagca gtgccattgc aatatgcatc ggtttcatac tttctctacc
    28021 tccgggcgac ccagaatacc ggtcggcatc accagcagca ccagaatcag cagggcgaat
    28081 gagaccacat ctttatattc cgtactcaga taggcagaag agagcgcctc cgcaatcccc
    28141 aggatcagcc cgccaatcat cgcccctgga atgctgccaa tcccaccgag caccgcagcg
    28201 gtaaaggctt tcatcccggc cataaagccg atataggggt taatgacgca gtagaactga
    28261 ccgagcagta cgccagccac tgccgccatc gccgcgccaa taacaaaggt caaagcaatc
    28321 acccggtcgg tgttgatgcc aagcaggctc gccattttca gatcttccgc gcaggcacga
    28381 caggcgcgcc ccatacggga atagcgaatg aaaatcgtca gcgccagcat ggcgaggaag
    28441 gtgacaatcc agatcaccgc ctgcatggtg gtaatagagg cagagaagtt ttcgctatgg
    28501 cccaccaccc actgaccgtt aaacaggctc ggcagcgcca cgtcgcgcga accttcggtc
    28561 aggctgacgt agttttgcag gaagatggac ataccgattg cggaaataag cgcaatcagg
    28621 cgcttagagt tacgcaccgg gcggtaagct acccgttcga tactccagcc gtaggcgctg
    28681 gcgatgacga ttgcgccgac gaatcccgcc gccaccagca gccagccggt atcaatgccc
    28741 atcatcatca gcgcggcgat gatcataaat gagacgtagc tgccaatcat ataaacctcg
    28801 ccgtgggcga agttgatcat gccgataatg ccgtaaacca tggtgtagcc gatggctatc
    28861 agcgcgtagg tactgcccag cgtgacgccg ttaaacatct gctgcaagaa atacaaaaac
    28921 tgctcagaca taaggtaacc tttctaaacc cgcccgcatt ttacgggcgg tgggatgatc
    28981 acttggctgc cgtggatgaa ccgtcggcgt gccactggaa gacaccaaaa tcaaatccct
    29041 taagatcgcc tttttcatcc cagttcagcg gcccaatcac ggtgtttgca ccgttagctt
    29101 ttaaatcttt caccagcgcc agcggctcat cgctgccggt acgctcaagg gcagttgcca
    29161 gagattgcac tgccgcgtaa gtgatccaga cataaggccc ggacggatct ttcttgtctg
    29221 ctttcagcgc atcaacgatg ccctggtttg ccggatcctg gtcatagcgt tttggcatag
    29281 tgaccaacat gccttcggcg gcatcaccgg caatgttcga cagcgatgcg ttaccgacac
    29341 cttccggccc cataaactgg gttttcaggc caacggaacg ggcttggcgc agcatctgcc
    29401 ccatttccgg gtagtaaccg ccgtagtaaa cgaagtcgat attttctttt ttcaggcggg
    29461 cgatcagcgc ggagaaatct ttctccccgg cggtaatacc gtcgaagaag acgacgttgg
    29521 cgttagccgc tttcagcccg tcctgcaccg aacgcgccag cccttcgcca tactgttgtt
    29581 tgtcgtgaat gatggcgatg cgctggggct tcaccgtctc aagaatgtat tttgccgccg
    29641 taggcccctg ggaagagtcc agcccggcag tacgcataat gtgttgataa ccgcgttggg
    29701 tcagctccgg gttggtcgct cccggcgaga tcatcagaat accttcgtct tcgtagatat
    29761 ccgacgctgg ctgggtagag gaagaacaca gatgaccaat aacgtattta atgccgtcgt
    29821 taacgatttt gttggcgacc gcaacggctt gtttcgggtc gcaggcgtcg tcatattcca
    29881 cgccaaccag cttgtcgccc ttaattcccc ctttgacatt aatgtcttta atcgcctgac
    29941 gcgcgccgtt aaattccata tcgccccact gggcaatcgg gccggacatc gcgccgacaa
    30001 cggcgacttt aatatcgtca gccatagcgg tgtgtgaaat tgccagtgca atcatccctg
    30061 cgatgatagt tttcgcattc cgtttcatag tcaaaaatcc ccattcgtga tgttgtgttg
    30121 ctttgttttt atgtgttaac aaatcagact gttctttttt tatactgcac tgtttttgcc
    30181 tgtctgatta aggggttagc gcagtatttt gtgatgatag cgattaaaat ccctattttt
    30241 cagtcgatta agaacagata atattctgaa tttattgata gataaacaga aaaaagtgcc
    30301 tttgtcagca taaaataacg gcacaaaggg cggaataatt cactatcatt caggggatta
    30361 tgctggacat ttttcattct ctaatgtttt aactttgtaa ttattgctgt taaaaaatta
    30421 atcacctgcc aaaagaaata aaaaagagaa agcctccgat taaattattt cgctacactg
    30481 gttccacttt tgtgatttac acgggttacc catgaagctg accatcattc gattagaaaa
    30541 ctttagcgac caggaccgga ttgacctgca aaagatctgg ccggagtatt ccccttcctc
    30601 tttacaggtt gacgataacc accgtatcta cgccgcgcgt tttaacgagc gtctgcttgc
    30661 ggccgtgcgg gtaaccttaa gcggcaccga gggagcactg gattctctac gcgtgcggga
    30721 agtcacccgc cgtcgcggtg tggggcaata tctgctggaa gaggttttgc gtaacaatcc
    30781 tggcgtttca tgctggtgga tggcggatgc aggcgtggaa gatcgcggtg tgatgacggc
    30841 gtttatgcag gcactgggat ttacggcaca gcagggcggc tgggagaagc gttaatcgtc
    30901 aagttgattt caaaagtgat gaccaacgag aaattgcctg atgcgctacg cttatcaggc
    30961 ctataatgtg tgctgcaatt tactgatttc tttggttctt gtaggccgga taaggcgttt
    31021 acgccgcatc cggcatgaag caacgtattc gatattagca atttggcggc aacccaaagt
    31081 tgccgattaa caattacttc gcatcggtcg ccgtgccgtt ggcgtgccag tcaaatacgc
    31141 cgaactcaaa gccctttaga tcgcctttct catcccaggt cagtggcccc atcacggtat
    31201 ccacggagtt cgcttttagg tatttggcga tttcagccgg atcgtcagac tgattcaggc
    31261 ccgcctgcaa agattgcagc gcggcgtagg tggtccagac gaatgcgccg cttgggtcct
    31321 gttttttcgc tttgatcgca tcaacaatgg gtttgttcgc cggaacctga tcgtagttct
    31381 tcggcttggt caccagcagc ccttccgctg attcgcccgc aatgttagac agcgaaacgt
    31441 tagccacacc ttccggcccc ataaactgag ttttcagccc tgccgcgcgt gcctgacgca
    31501 ggatttgccc catttccggg tgataaccgc cgtagtaaac gaagtcgata ttctcttttt
    31561 tcagacgcgc caccagcgtt gagaaatctt tttccccggc ggtgatacca tcaaagaaca
    31621 ccacgttagc attgcctttc ttcaggccgt cctgcaccgc tcgtgccaga ccttcgccgt
    31681 attgctgttt gtcgtgaacg atagcaatac gctgcggttt cactttctca agaatatatt
    31741 tcgccgccgt cggcccctgg tcggagtcca ggccggtggt gcgcaggatc agctgatagc
    31801 cacgggcggt cagctccggc gcggttgccg ctggggtgat cattaaaatg ccttcgtctt
    31861 cgtagatgtc agacgcaggc tgcgttgatg aagaacagag gtgaccaatc acatatttaa
    31921 tgccgtcgtt aacgactttg ttcgccaccg caaccgcctg tttcgggtca caggcatcgt
    31981 catattttac gatttgcagt ttgttgcctt taatgccgcc tttagcgttg atatccgcaa
    32041 ccgcctgctc tgcgccggta aactcctggt caccgtactg cgcaaccgga ccggacattg
    32101 cacccacgac cgcgacttta atatcttctg ccagagccat attgctgaat gccagcgcga
    32161 tacatcctgc cagtaacgct ttacccttta tgttcatcct gagaatcccc attcttctgg
    32221 ttattacgtg tgttgtgatg ttgttgttca gcactttatt tcgttttatg catgactacc
    32281 cgtgctttag cagcatactc tgctaaaaca tacccgattt ttatgatatt ggaatagcta
    32341 ttttgacagt ttattaacaa tctgcgtggg gattggcgtt ttgccggagg gggaattgat
    32401 tattgtagag gcccaaaaaa caaaaacccc ggactctcat ccagggttct ctgcttaata
    32461 gcggaaatta cgcttcaata gcggcgcgaa gttttttcat cgcgttcttt tccagttggc
    32521 gcacacgttc agcggaaaca ccgtaacggt cagccagttc ctgcaacgtg gacttgttgt
    32581 cttcgtccag ccagcgcgca cggatgatgt cctggctgcg ttcgtccaga ccatgcatcg
    32641 cgtcggtcag acggtttgcc gcctgctctt cccagttatc atcttcgatg ccgtcggcaa
    32701 agttagatga tttatcctgc agatagagca ccggtgccat cggctggctg tcggaatcgt
    32761 cgtcggaaga caggtcaaag gtcatgtcct gtgccgccat acgtgattcc atctcacgta
    32821 cgtctttgct ggttacgccc agttcacggg ccaccatttc gacttcatcc tggttaaacc
    32881 agcccagacg ctgcttggtt ttacgcaggt tgaagaacag tttgcgctgc gctttggtgg
    32941 tcgcaacttt gacgatacgc cagttacgca gaacgtattc gtggatctct gctttgatcc
    33001 agtggacggc gaaggagacc aggcgcacac ccacttccgg gttaaaacgg cgcactgctt
    33061 tcatcaggcc gatgttacct tcctgaatca aatccgcctg tggcaggcca tagcccgcat
    33121 aattacgagc aatatgaaca acaaaccgca ggtgagacag gatcagcgtt ttagctgctt
    33181 ccagatcgcc atggtaatgc agcttttcag ccagcgcccg ctcctcgtca gccgacaaca
    33241 tcggccacgc gttagctgcc cggatgtagg aatccaggtt gccaactggg gctaaagcta
    33301 aactttgcat tttgtcagtc attcaaatcc tctcaatcga tatcttctgg cgcttcagtg
    33361 gtagcaacaa ctgtgccaaa gcttaagagc aacgaggtta tcattcactg ttttatcaga
    33421 ccgtgatttt atccacaagt tcaatgcaag cttgtgaata aattacgcac aaaatgtgac
    33481 atagagatga aataccggga agagacaacg gggtctcttt ccctgctacg gaacccattg
    33541 cagggaaaga gtataacacg cttttattat tcaggcgtaa agtggcgtaa atgttgtacc
    33601 gtggcaagcc acgctgccac ccagccaatc atcgagcata ccagcagcaa tagcaggcat
    33661 tcatcgaacg ataaaccatt gatatcaaac ttcgttccga aaacctgtgt cacttccgcc
    33721 accgctgacg acaaacgcag taccagaatt tctgacaaaa ttaatgacaa caatgcgcca
    33781 gaaaatccca gcagcgcacc gccgtacagg aacgggcgaa ggatgaaacc atccgtcgca
    33841 ccaatcagtt tctgcacgtt gatggagtca cggcgagcaa agatgctcag acgtacgctg
    33901 ttgccgatga cgaggaacac tgccgccacc atcaacacgc cgatcatcgc cgaaacgcgc
    33961 ccaaccagcc cggtcaacgc cgccagacgg gcaaaccagc tgtcatccat ccgcacttcg
    34021 tcaataccgt taatctgcgt gatacgatca cgcagcgtat tgagtgattc cgtcccctga
    34081 aaatcgagtt tcgggatcac caccgccact gccggaagcg ggttttcttc cagcatatcc
    34141 agcgcaccac caaaaccaga ccagttacgg aactcgccca gtgcgtcttc acgagaaaga
    34201 tagttcactt tctccacgcc ttgctcggcc tgcaactgtg ccaccacgcc cgcagcggcg
    34261 tcatcgtcca gcgttttttg caagtaaaca gtgatttgcg gtgacggata atactgcgtc
    34321 gccgcctgat taacgttttt gtacaccata taacagacgc tgggcagcgt cagggagatg
    34381 gcgataacca tcaccgttaa aaacgtggcg aacggtttgc ttttcagatc ctgcaatgcg
    34441 ccgtggaagg catagcgcac ctgttcgttg aaaacgttgg ttttgcgatt taccggtttt
    34501 ggcgaggatt tcgcgcgttt cggtgcgtta cgaccgccgt cgcctgagcc gccgaccgat
    34561 ttacggaagc gatccagacg cccaccaaac tgccgaatat gattgattgc atcgcgctta
    34621 ttcatggccc acgcctccat gcaagtgacc atcgctcagg gtgagcatgc gataggaacg
    34681 ccgtgaaatc agattaatgt cgtgcgttgc catcaatacg gttaccccaa cgcggttaaa
    34741 ctcttcaaac agacgtagaa tcccttccga caacgcgtcg tccaggttac cggtcggttc
    34801 gtccgccagc agtaccgcgg gcttattcac caccgcgcgg gcaatgccaa cacgctgttg
    34861 ttcaccgccg gaaagctgaa tagggaagtt cttcgctttg tccagtagcc cgactttatc
    34921 cagtgccgcc gacacccggc gacgaatatc gtcaccgctg gcaccagcga taatcagcgg
    34981 gatcgccacg ttatcgtaga cagtacggtc catcagtaga tggtgatcct ggaaaatcat
    35041 gccaatctgg cggcgcagaa acggaacttc acggtttttc agacgcgtga tgtcatggcc
    35101 gctaaaccag attttcccgg cgctgggccg ctcaatccca cagatcagct tcaggagggt
    35161 acttttccct gcgccggaat gaccggtcag aaacgccatc tcacccggct gcatatggaa
    35221 cgtaacgccc tgcagcgcct gtctcccacc gagataagcc ttgctgacat gttcaaagcg
    35281 aatcattgtt aatcctctcg ggcaaaaagt gcctctataa agtcgtccgc cttaaacgga
    35341 cgcaaatcct caatacgttc gccgacacca atgtagcgga tagggatacc aaactggtca
    35401 gccaccgaga aaattacccc gcctttcgcc gtgccgtcca gtttcgttag cgtgatgccg
    35461 gttaagccaa cagcttcgtg gaacagtttg gcctggctta ccgcgttctg cccggtgctg
    35521 gcatcaatag tcagcataac ttcatgcggc gcttcaacgt cgagtttctt catcacgcgg
    35581 acgattttct tcaactcttc catcagatgc gatttgttct gcagacgtcc ggcggtatcg
    35641 gcaatcagga cgtcgatatt acgcgcttta gctgcctgaa tggcgtcgaa gataacagag
    35701 gcggaatccg caccggtatg ctgggcaatc accggaatat tgttgcgctg accccagacc
    35761 tgaagctgtt caaccgcagc tgcacggaaa gtatcgcccg ccgccagcat caccgattta
    35821 ccctgctgct caaactgacg cgccagctta ccaatcgtcg tggttttacc cacaccgttg
    35881 acgcccacca tcaggatcac gaacggcgtt ttatcttcaa cattcagcgg ctcatcgact
    35941 ttcgccagaa tctcgcccat ctcttctttc agcaggccat agagcgcctc ggcatcacga
    36001 agctgcttgc gggatgcgcc ttccgtcaga ttggtgataa ttttacgtgt ggtttccacg
    36061 cccacatcgg cgatcaacag ctgttcttcc agctcttcaa acagatcatc gtcgattttt
    36121 ttaccgcgga acaggctgat aaatccggaa ccgagatttt ctttggtttt taacaggctg
    36181 cgtttcaggc gcgcgaagaa gccttctttg gtcggttttt cttgctcctg ggcaatttct
    36241 gcaaccggct gctcttcttc tgccggagga actaccatca ccgcctcttc tgccgcttcg
    36301 gcagccagcg cctgtgcttc cagctcttcg tcggtgattt cttctttagc cgcttcttct
    36361 tctgccgctt cgacaatctc tacggtttcc gcttcagcct gccactcttc tggcgaaacc
    36421 tcttcggcgt tgacgtcttc cggcaacggc agctcttcac gttcgatagc cactggctcc
    36481 ggcgtttctt ctacgaccgg ttccggctgt gcaacaactt ccgcttcagg ctgcgctttt
    36541 tcactttcaa caacctgttc agtgacttcc acaacgtcgg cagcaaaagt ttccgcctcg
    36601 gcttcagtat gcgcctgcgg ctgctcttca acggcttgtt cagaagcctt cgcaggctct
    36661 tgcgcctgaa cgatttcttc tacaaccggt tgttcattct gaacttctgt ctctttttcc
    36721 ggggtctgct ctttttgacc aaagcccagc caggaaaaaa agccacgtct tttttctttc
    36781 gccatttgcg actacactcc tcgctgttgc ttcatggcac agcgttaacg ctatgtacat
    36841 agcagctaaa aaaatgatga aatagtctat cacttaactt aattcacatc accgcctgca
    36901 agtatgtgtt atctggcgga ttgagcaatt tatcatgaaa aaaccgaatc attccggcag
    36961 cggccaaatc cgcattattg gcgggcagtg gcgaggccgt aaactcccgg ttcctgatag
    37021 cccaggtctg cgccccacca ccgaccgcgt acgcgaaacg ttgtttaact ggctggctcc
    37081 ggtcattgtt gacgcccaat gtctggattg cttcgccggg agcggcgcgc tggggctgga
    37141 agcgttatcg cgctacgctg cgggggcaac gttgattgag atggatcgcg tggtttctca
    37201 gcagttaatt aagaatctgg cgacactaaa agcaggcaat gcacgcgtgg tgaacagcaa
    37261 cgcaatgtca ttcctggcgc aaaaaggcac accgcataat atcgtgtttg tcgatccacc
    37321 gttccgccgt ggcttgttag aagagacgat aaatttactg gaagataacg gctggctggc
    37381 tgacgaagcc ctgatttatg tcgaaagcga agtcgaaaac ggtctgccca ctgttccggc
    37441 aaactggtca ttacatcggg aaaaagtggc gggtcaggtg gcttatcggc tgtatcaaca
    37501 cgaagcacaa ggagaaagtg atgctgatta atattggtcg tttgttaatg ctctgcgttt
    37561 ggggattttt aatcctcaat ctggtgcatc ccttcccgcg cccgctaaat attttcgtta
    37621 acgtggcgct gatttttacc gtgctgatgc atggtatgca gctggcgcta ttgaaatcca
    37681 ctttaccgaa agatggcccg cagatgacca ccgccgaaaa ggtgcggatt ttccttttcg
    37741 gcgtgtttga actgctggtc tggcagaaga aatttaaagt taaaaaataa ccttattgtt
    37801 cgccaacaaa gctaacaaag cgcgttcctt tatagctcag cgtaccttta tcgcccactg
    37861 tcagggcgtg gtactgctgg gcgtcgaggc gaaacgtctg ctccattcct ccgctttgcg
    37921 gtttgaagct tgcctcatag cgcatactgg tgcctgccgg agtcacttcc tgctggcgcg
    37981 aacggcgatc gttaatcggt ttttcccgct tgttgctcac caccaccagc ttttgctgga
    38041 gcggagccat atcattatca gctttttccc gtcgctgttg cataaaacga aacgatgcgg
    38101 cgacgacaat taagccaatg ataacaataa agaaaagagg tggtttactc atctttatcc
    38161 ctcatcggaa aatgcggaat taagcatacc ctgccagtta tggtgttgtc atccgtccac
    38221 cctcgccact aaactggaag caagaccgta ggcattctgc ttacgaaaaa ataacgaatt
    38281 caaggaacta agatgctttg gtcgtttatc gctgtctgtc tttccgcatg gctatctgtg
    38341 gatgcatcgt atcgtgggcc aacctggcaa cgctgggtgt ttaaaccgtt aacccttctt
    38401 ctcctgctgt tactggcctg gcaagcaccg atgttcgacg ccattagcta tctggtgctg
    38461 gcgggactgt gcgcctcact gctgggcgat gcgctaaccc tgttgccacg tcaacgtctg
    38521 atgtacgcca tcggcgcgtt tttcctctcg cacctgctgt acaccatcta tttcgccagt
    38581 cagatgacgc tctctttctt ctggcctctg ccactggtgt tgctggttct gggtgcgctg
    38641 ctactggcga ttatctggac gcggctggaa gagtaccgtt ggcctatctg cacgtttatc
    38701 ggcatgacgc tggtgatggt gtggctggca ggtgaactgt ggttcttccg tccgaccgct
    38761 ccggcgctct ctgcgtttgt tggcgcttcg ttgctgttta tcagtaactt tgtctggctg
    38821 gggagccact atcgccgacg cttccgtgcg gataacgcga ttgctgcggc ctgctacttt
    38881 gccggtcact tcctcatcgt ccgctcactg tatctctgat aaaacttgac tctggagtcg
    38941 actccagagt gtatccttcg gttaatgaga aaaaacttaa ccggaggatg ccatgtcgac
    39001 acctgacaat cacggcaaga aagcccctca atttgctgcg ttcaaaccgc taaccacggt
    39061 acagaacgcc aacgactgtt gctgcgacgg cgcatgctcc agctcgccaa cgctctctga
    39121 aaacgtctcc ggcacccgct atagctggaa agtcagcggc atggactgcg ccgcctgtgc
    39181 gcgcaaggta gaaaatgccg tgcgccagct tgcaggcgtg aatcaggtgc aggtgttgtt
    39241 cgccaccgaa aaactggtgg ttgatgccga caatgacatc cgtgcccagg ttgaatctgc
    39301 ggtgcaaaaa gcgggctatt ccctgcgcga tgagcaggcc gccgacgagc cacaagcatc
    39361 gcgcctgaaa gagaatctgc cgctgattac gcttatcgtg atgatggcaa tcagctgggg
    39421 tctggagcag ttcaatcatc cgttcgggca actggcgttt atcgcgacca cgctagttgg
    39481 gctgtacccg attgctcgtc aggcattacg gctgatcaaa tccggcagct acttcgccat
    39541 tgaaacctta atgagcgtag ccgctattgg tgcactgttt attggcgcaa cggctgaagc
    39601 tgcgatggtg ttgctgctgt ttttgattgg tgaacgactg gaaggctggg ccgccagccg
    39661 cgcgcgtcag ggcgttagcg cgttaatggc gctgaaacca gaaaccgcca cgcgcctgcg
    39721 taacggtgag cgggaagagg tggcgattaa cagcctgcgg cctggcgatg tgattgaagt
    39781 cgccgcaggt gggcgtttgc ctgccgacgg taaactgctc tcaccgtttg ccagttttga
    39841 tgaaagcgcc ctaaccggag aatccattcc ggtggagcgc gcgacgggcg ataaagttcc
    39901 tgcaggagcc accagcgtag accgtctggt aacgctggaa gtgctgtcag aaccgggtgc
    39961 cagcgccatt gaccggattc tgaaactgat cgaagaagcc gaagagcgcc gcgcccccat
    40021 tgagcggttt atcgaccgtt tcagccgtat ttatacgcca gcgattatgg ccgtcgccct
    40081 gctggtaacg ctagtgccgc cgctgctgtt tgccgccagc tggcaggagt ggatttataa
    40141 aggtctgacg ctgctgctga ttggttgccc gtgtgcgtta gttatctcaa cgcctgcggc
    40201 gataacctcc gggctggcag cagcagcgcg tcgtggggcg ttaattaaag gcggtgcggc
    40261 gctggaacaa ctaggtcgtg ttacccaagt ggcgtttgat aaaaccggta cgctgaccgt
    40321 cggtaaaccg cgcgttaccg cgattcatcc ggcaacgggt attagtgaat ctgaactgct
    40381 gacactggcg gcggcggtcg agcaaggcgc gacgcatcca ctggcgcaag ccatcgtacg
    40441 cgaagcacag gttgctgaac tcgccattcc caccgccgaa tcacagcggg cactggtcgg
    40501 gtctggcatt gaagcgcagg ttaatggtga gcgcgtgttg atatgcgctg ccgggaaaca
    40561 tcccgctgat gcatttgctg gtttgattaa tgaactggaa agcgccgggc aaacggttgt
    40621 gctggtagta cgtaatgatg acgtgctggg tatcattgca ttgcaggata ccctgcgcgc
    40681 cgacgctaca actgccatca gtgaactgaa cgcgctgggg gttaaaggag tgatcctcac
    40741 cggcgataat ccacgcgcag cggcggcaat tgccggggag ctggggctgg agtttaaagc
    40801 gggcctattg ccggaagata aagtcaaagc ggtgaccaag ctgaatcaac atgcgcccct
    40861 ggcgatggtc ggtgatggta ttaacgacgc gccagcgatg aaagctgccg ccatcgggat
    40921 tgctatgggt agcggcacag acgtggcgct ggaaaccgcc gacgcagcat taacccataa
    40981 ccacctgcgc ggcctggtgc aaatgattga actggcacgc gccactcacg ccaatatccg
    41041 ccagaacatc accattgcgc tgggactgaa agggatcttc ctcgtcacca cgcttttagg
    41101 gatgaccggg ttgtggctgg cagtgctggc agatacgggt gcgacggtgc tggtgacggc
    41161 gaatgcgtta aggttgttgc gcaggagata aggcaaaccg atcgcaacat tgagcgcgat
    41221 cggtcccctc gcccctctgg ggagagggtt agggggtgag gaaaaggcgg catcgaagcc
    41281 aatcagcccc tatcaaccgc ctttacgaat caaataacga taaggcagtc catccgtctc
    41341 tttcgcaacc agttcgtgtt ccataaaggt acaaaaccca ggaatatcgc gggtagtggc
    41401 cggatcgtcg gcgataatca gcaacgtttc gccaggctgc atattgcgca cggttttgcg
    41461 caccatcatc accggttccg ggcagcgcag gccaagcgcg tcgagtgtgt ggtcagggct
    41521 ggaaaagaga tcggtcattt tcttctcatc acttaaaaaa acggcgctag tttacgccct
    41581 gtgagtccgt aagcaaacca ggttaacgat tgcgtgaaaa ttaaccattg cattgtcaac
    41641 gtaaagcagt atcatgcggc ggctcgaaaa aagggtaagc acgttattat gttaaggtaa
    41701 cagacgtgtc gtacgtattg ggttccctca ccccaatggt taatcaaaaa ggtacaatat
    41761 gaacgttttc tcgcaaactc aacgctataa ggcgttgttc tggttatcgt tatttcatct
    41821 tctggtgatc acctccagta actatctggt tcagcttccc gtctccattt ttggtttcca
    41881 taccacctgg ggcgcgttta gctttccgtt tatttttctt gctaccgacc tgaccgtgcg
    41941 tatttttggc gcaccgctgg cccgacgcat tatcttcgcg gtaatgatcc ctgcgttatt
    42001 aatctcctac gtcatctcgt cgctattcta tatgggttcc tggcagggat tcggcgcact
    42061 cgcccacttc aacctgtttg tcgcccgtat cgccaccgcc agtttcatgg cctacgcgct
    42121 ggggcaaatc ctcgacgtgc acgtttttaa ccgcctgcgt cagagtcgcc gctggtggct
    42181 ggcaccgaca gcgtccacac tgttcggtaa cgtcagcgac acgctggcct ttttcttcat
    42241 tgccttctgg cgtagcccgg atgcctttat ggctgaacac tggatggaaa tcgcgctggt
    42301 cgattactgt ttcaaagtgt taatcagtat cgttttcttc ctgccaatgt atggcgtatt
    42361 actcaatatg ctgttgaaaa gactggcaga taaatccgaa atcaacgctt tgcaggcgag
    42421 ttaaaggttc gttatcagag ttgtgataag atggatgaat gagccgttat ggccgtttat
    42481 cgaaaggaag aagtcaatgc gcaatctggt taaatatgtc ggaattggcc tgctggttat
    42541 ggggcttgcg gcctgtgatg ataaagacac taacgctacg gcgcagggtt cggttgcgga
    42601 aagtaacgct accgggaatc ccgtcaacct gcttgatggc aagttaagtt tctcgctgcc
    42661 agcggatatg accgaccaga gcggtaagct gggaacgcag gccaataaca tgcatgtctg
    42721 gtccgacgcc accgggcaga aagcagtcat cgtcatcatg ggcgatgatc cgaaagaaga
    42781 tctggcggtg ttggcgaagc gtctggaaga tcagcaacgt agccgcgatc cgcagctgca
    42841 agtggtaacc aataaagcca ttgagctgaa aggtcacaaa atgcagcagt tagacagtat
    42901 tatctccgcg aaaggccaga cggcgtactc ttccgttatt ctgggtaacg tgggtaatca
    42961 actgctgacc atgcaaatta cgctgccagc tgacgatcag caaaaagcgc aaaccaccgc
    43021 agaaaacatc attaatacgc tggttattca gtaagtttta agatgatgag gcggcctcag
    43081 ggacgtgttc cggaggccgt ttttttaatc gccacgtcag taataacgcg attgcgacca
    43141 gtcccgccgc cgccagataa atcaccggta cgcccgccca gctcatcacc agcccagcca
    43201 gtggtccagt cacgccaagc gataaatcca taaatacggt gtaagttgcc agcgccgccc
    43261 cctgattttg ctgcggaacc gcttttaccg ccactacacc caatgccggg aacaccagcg
    43321 aaaacccggc ccccgccagt aagacgccga ttttcgccat ccacggcata gtcgccacgc
    43381 caaccagtag caggccgatt atctcaacgc taaagcaaat catcgctacg tttaagccac
    43441 cgatacggtt aatgccgtta gggaataaca aacgcgtacc gacaaacgca cagctaaaca
    43501 gcgtcagcgc gaaagccgca ccgtcccaac ctttagcgtc ataaaacagc gtgataaagg
    43561 tggcgatgac gccaaatccg gcggaagcca gtgccagcgc cataccgtac agccagacgc
    43621 gcccaagcac cgcgcgaaac ggcagcggtt tgcctttact ggcttttacc gtcggacgcg
    43681 ggatcgccaa caaaatggcc accagcgcca cgcccataat gattaacgct aacgcctgca
    43741 agccgcccca gtgataaaac acgacgccta acggcgcacc catcgccatc gccccgtaag
    43801 tgacaatgcc gttccacgaa atcacccgcc cgatatgcag cgagccaacc actccaacgc
    43861 cccatagggt cgatcccgtt ccggcaaaac tttgcccaat cccaaggatg acgcgcccca
    43921 ggcaaagtaa taacaggctg atgacaggca gactggcggt taatcctgcc gtcagatacc
    43981 ccagaccgct caaaaagcag ccgcataaac cgaagacgac aatctttttg ggtcccagca
    44041 aatcggcgta acgtccggca tgagggcggc tcagcaaggt ggcgaaatat tgcaggctga
    44101 taaccaatcc tgcccagaag gcgctaaagc ccatcacatc atggacatag cccggtaata
    44161 cagcgagcgg caacccgatg gtgaggtagc tggcgaagtt aaacatgaca atggagacaa
    44221 tgcgcaaatt caggcgcaat ccgtttagcg cgggttcggc tacgggttcg ggcatgagga
    44281 tcaccacatt tttacaacag tgtttcattt ttaccatgtg ctgacgtgaa aatcagcagt
    44341 aagaatcaga atattgctgg cgtgactccc gcgctacact taacacaaaa agtcacaagg
    44401 aagccccaat ggaaacccct caacccgata aaacgggcat gcacattctg ctcaagttgg
    44461 cctcgctggt agtgatcctc gcgggcattc acgcagcggc agatatcatt gtgcagctgt
    44521 tactggcgct gttttttgcc atcgtcctca acccgctcgt tacctggttt attcgtcggg
    44581 gagtacaacg ccccgttgcc attacgattg tagtggtggt gatgctgatc gcactaaccg
    44641 cgctggtcgg cgtactggcg gcatcgttta acgaatttat ctctatgctg ccgaagttta
    44701 ataaggagct gacgcgcaaa ctttttaaat tgcaggagat gttgcctttt cttaatttgc
    44761 atatgtcgcc ggagcgaatg ctgcagcgga tggactcgga aaaagtggtt accttcacca
    44821 cagcgctaat gaccgggctt tccggggcaa tggcgagcgt gcttttgctg gtgatgaccg
    44881 tagtttttat gctgtttgaa gtgcgccacg tcccttacaa aatgcgtttt gcgctgaata
    44941 acccacagat tcacatcgca ggattacacc gcgcacttaa aggcgtttcg cattatctgg
    45001 cgttaaagac gctactcagt ttatggacag gtgtcatcgt ctggctgggg ctggcgctaa
    45061 tgggcgtaca gtttgcgctg atgtgggcag tactggcgtt tttgctcaac tacgtgccca
    45121 atatcggcgc ggtaatttcc gccgtaccgc caatgattca ggtgctgctg tttaatggtg
    45181 tttacgaatg tattctggtc ggcgcattgt ttttagtggt ccatatggtc atcggcaata
    45241 ttttagaacc acggatgatg ggccatcgcc tggggatgtc caccatggtg gtatttcttt
    45301 cattgttaat ctggggatgg ctgctcggcc cggtagggat gctactttcg gtgccattaa
    45361 ccagcgtgtg taaaatctgg atggaaacga cgaaaggtgg cagcaaactg gcgattttac
    45421 tggggccggg cagaccgaaa agtcggttac cgggatgagg cgacaagtga tacgatacgc
    45481 actttcattt tccattaaac gttggccctg atatgtatcg gatagttctg gggaaagttt
    45541 cgaccttaag cgcagctcca ctgccaccgg gtttacgcga gcaagcaccg caaggtccac
    45601 gacgcgaacg ctggctggcg gggcgtacat tgctttcgca cacgctttcc ccgctaccgg
    45661 agatcatcta tggcgaacaa ggcaaacctg cattcgcgcc ggaaacgccg ctatggttca
    45721 acttaagcca tagcggtgac gatatcgctc tgctgttgag cgatgaaggc gaagtcggct
    45781 gcgatatcga agtgattcgc ccgcgcgtca actggcgctg gctggcgaac gccgtgttca
    45841 gcctcgggga acacgctgaa atggacgccg tgcatcctga tcagcaactg gaaatgttct
    45901 ggcgcatctg gacgcgcaaa gaagccatcg ttaaacagcg tggcggcagc gcctggcaaa
    45961 tcgtcagcgt agatagcacc tatcactcct cgctgtcagt cagccattgt cagctagaaa
    46021 atttaagcct tgcgatctgc acccccaccc cctttacgct caccgccgac agtgtgcaat
    46081 ggatcgattc agttaactga tccgcccacc cgactgccca tctattgatc cagaacaggt
    46141 aatcagtatg acgaatactt aaaatcgtca tacttatttc cgccatctat tttaatccat
    46201 tggggttacc atgctctcca cactccgccg cactctattt gcgctgctgg cttgtgcgtc
    46261 ttttatcgtc catgccgctg caccagatga aatcaccacc gcctggccgg tgaatgtcgg
    46321 gccactaaac ccgcaccttt acacgcctaa ccagatgttc gcccagagca tggtttatga
    46381 accattggtg aaatatcagg cagacggttc ggtgatcccg tggctggcaa aaagctggac
    46441 tcattcagaa gatggtaaaa cctggacctt caccctgcgt gatgacgtga aattctccaa
    46501 cggtgaaccg ttcgatgccg aggcggcggc agaaaacttc cgcgcagtgc tcgataaccg
    46561 tcaacgtcac gcctggctgg agctggcaaa ccagattgtt gatgttaaag cactcaataa
    46621 aacagagctg caaattaccc tgaaaagcgc ctactatcct ttcctgcaag aactggccct
    46681 gccccgtcct ttccgtttta tcgctccctc gcagtttaaa aaccatgaaa ccatgaacgg
    46741 aattaaagcg ccgattggca ccggaccgtg gattttgcag gaatcgaaac tgaatcagta
    46801 cgatgtcttc gtccgtaacg aaaactactg gggcgaaaag ccagcgatta aaaagatcac
    46861 ctttaacgtc atcccggacc cgactacccg cgcggtggcg tttgaaactg gcgatatcga
    46921 cctgctgtac ggaaacgaag ggttattacc gctcgatacc ttcgcccgct ttagccagaa
    46981 tccggcttac cacacccaac tgtcacagcc gatcgaaacc gtgatgctgg cgctcaatac
    47041 cgccaaagcc cccaccaacg agctggcagt acgtgaagct cttaattacg cggtaaacaa
    47101 aaaatcgctg attgataacg cgttatatgg cacccagcag gtcgccgaca ccctgtttgc
    47161 cccttctgtg ccttacgcta atctcggcct gaaaccgcgc cagtacgatc cgcagaaagc
    47221 gaaagagttg ctggaaaaag ccggttggac gctgcctgcg ggcaaagaca tccgcgagaa
    47281 aaatggtcag ccgctgcgca ttgaactgtc gttcatcggc accgatgcgt taagcaaatc
    47341 gatggcggaa atcattcagg ctgatatgcg ccagattggc gcagatgtct cgctgattgg
    47401 cgaagaagag agcagtatct atgctcgtca gcgcgacggt cgttttggca tgattttcca
    47461 ccgcacctgg ggcgcgccat acgatccaca cgccttcctg agttcaatgc gcgtaccgtc
    47521 gcacgctgac ttccaggcac agcaaggatt agccgacaag ccgctgattg ataaagagat
    47581 cggcgaagtg ctggcgaccc atgacgaaac gcaacgtcag gcgctgtatc gcgacattct
    47641 gacccgtctg catgacgagg cggtttatct gcctatcagc tacatctcga tgatggtggt
    47701 atcaaaaccg gagctgggta atatccccta cgcgccgatc gccaccgaaa ttccgttcga
    47761 acagattaaa ccggtgaaac cttaatgttg cgttacgtat tacgccgctt tctgctgctg
    47821 atcccgatgg tgctcgccgc ctcggtgatc atttttctga tgctgcgcct cggtaccggc
    47881 gacccggcgc tcgattatct gcgtttatct aacctgccgc cgacgccgga gatgctggcc
    47941 tctacccgca ccatgctcgg gctggatcag ccgctgtacg tccagtacgg tacctggttg
    48001 tggaaggcgc tgcatcttga ctttggtatc tcattcgcca gccaacgccc ggtactggac
    48061 gatatgctga acttcctgcc cgccacgctg gaacttgcag gtgcggcgct ggtattaatt
    48121 ctgctcactt ccgtaccgct cggtatctgg gcggcgcgcc accgcgatcg tttgccggat
    48181 ttcgccgtgc gctttatcgc gtttcttggc gtgtcgatgc ccaacttctg gctggcattt
    48241 ttgctagtga tggcgttttc ggtgtatctg caatggctac ccgcgatggg ctacggcagc
    48301 tggcagcaca tcattttacc tgcggtttcc atcgccttta tgtcgctggc gattaacgcg
    48361 cgactgctgc gcgccagtat gctggacgtc gccggtcagc gtcacgtcac ctgggcgcgg
    48421 ctacgcggcc tgaatgacaa acagaccgaa cgccgccata ttctgcgcaa tgcctcgctg
    48481 ccgatgatca ccgccgtggg gatgcacatt ggcgaattga ttggcgggac gatgattatc
    48541 gaaaacatct ttgcctggcc tggcgtcggg cgctatgcgg tgtcggcgat ttttaaccgt
    48601 gactatccgg tgattcagtg ctttacgctg atgatggtgg tggtttttgt ggtctgtaat
    48661 ttgattgtcg atttgctcaa cgccgcgctg gacccgcgca ttcgtcgtca tgaaggagcg
    48721 cacgcgtgaa ctttttcctc tcttcccgct ggtcggtacg cctggcgctg atcattatcg
    48781 ccctgctggc gctgattgcg ctcaccagcc agtggtggca gccgtacgat ccacaggcaa
    48841 ttgatttgcc gtcgtgcctg ctttcgccgg atgcgcagca ctggctgggc accgatcact
    48901 taggtcgcga tattttctcg cggctgatgg cagcgacccg cgtgtcgctc ggttcggtaa
    48961 tggcctgcct gctgctggtg ctgacattag ggctggttat tggcggcagc gccgggttga
    49021 ttggcgggca cgttgatcag gccaccatgc gcgtcgccga tatgtttatg accttcccga
    49081 cctcgattct gtcgttcttt atggttggcg tgctcggcac cgggctgacc aacgtaatta
    49141 tcgccatcgc cctgtcgcac tgggcgtggt atgcacgcat ggtgcgcagc ttggtgattt
    49201 cactacgcca acgcgagttt gtgctggcgt cacggctttc cggtgcgggc catgtgcggg
    49261 tgtttgtcga tcatctggca ggcgcggtga tcccttcgct gctggtgctg gcaacgctgg
    49321 atatcggcca tatgatgctg cacgtcgcgg ggatgtcttt ccttggcctc ggtgtgaccg
    49381 cgccgaccgc cgaatggggc gtgatgatta acgacgcgcg ccagtatatc tggacccagc
    49441 cgctgcaaat gttctggccg gggctggcgc tgtttatcag cgtgatggcc tttaacctgg
    49501 tgggtgacgc actgcgcgat catctggatc ctcatctggt gacggagcac gcacactaat
    49561 gccgcaacag attgaactac gtaatatcac gctacaggcc gcgcagccgc tggtacacgg
    49621 tgtatcgtta accctgcaac gcgggcgcgt gctggcgtta gtcggcggta gcggcagcgg
    49681 gaaatcatta acctgcgccg cgacgctggg cattttgccc gctggcgttc gccagacggc
    49741 gggggaaatt ttagccgatg gcaaaccggt ttcgccttac gccctgcgcg gcatcaaaat
    49801 tgccaccatc atgcagaacc cgcgcagcgc ctttaatcca ctgcacacca tgcacaccca
    49861 cgcgcgggaa acctgcctgg cgttagggaa acccgccgat gacgctacgc ttaccgctgc
    49921 catagaagcg gtggggctgg aaaacgccgc gcgcgtgctg aagctgtacc cgttcgagat
    49981 gagcggcggc atgttgcagc gcatgatgat tgcgatggcg gtgctgtgtg aatcaccgtt
    50041 tatcatcgcc gatgaaccga ccaccgacct cgacgtggta gcacaggcgc gcatcctcga
    50101 tctgctggaa agcattatgc aaaaacaagc gccgggaatg ctgctggtca cccatgatat
    50161 gggcgttgtg gcgcgtctgg cggatgacgt ggcggtgatg tcacaaggta aaattgtcga
    50221 acagggcgat gtagaaacgc tgtttaacgc ccccaaacat acggtgacgc gcagcctggt
    50281 ttccgctcat ctcgccctct acggtatgga gctggcatca tgaccttact taacgtctgc
    50341 ggcctttccc atcactatgc ccacggtgga tttagcggaa aacatcaaca tcaggcggtg
    50401 ctgaataacg tttccctgac cctgaaaagc ggcgaaaccg tcgccctgct gggacgcagc
    50461 ggctgcggaa aaagcaccct cgcgcggctg ctggtaggct tagagtctcc ctcacaaggg
    50521 aatattagct ggcacggcga atcgctggcg aaacttaatc gcgcccaacg taaagccttc
    50581 cgccgtgata ttcagatggt gtttcaggac tccatcagtg ccgtgaatcc gcgcaaaacc
    50641 gtgcgcgaga tcctgcgtga accgatgcgc cacctgctat cactgaaaaa atccgaacaa
    50701 ctggcgcgcg ccagcgaaat gctgaaggcg gtcgatctcg atgacagcgt tctcgacaaa
    50761 cgcccaccgc agttaagcgg cggccagctc cagctcgtct gcctggcacg cgcgctggcg
    50821 gtcgaaccga aattgctgat tctggatgaa gccgtttcta accttgatct cgtgttacag
    50881 gcgggcgtca ttcgcctgct gaaaaagcta caacaacagt ttggcactgc ctgcctgttc
    50941 atcactcatg acttacgcct ggtggaacgc ttttgccagc gagtgatggt gatggacaac
    51001 ggacaaatcg ccgaaaccca ggcggtggga gataaattaa ccttttcctc tgacgccgga
    51061 cgtgtgctac aaaacgcggt attacccgca ttccccgtgc gccgtcgcac ctcagaaaag
    51121 gtttaacgcc aatgcaacga gtcaccatca cgcttgatga cgatttactg gagacgctgg
    51181 acagcctgag ccagcgtcgt ggttataaca accgttccga agctatccgc gatattctgc
    51241 gtagtgccct ggcgcaggaa gctacccagc agcacggcac acaaggtttc gcggtgctgt
    51301 cgtatgtgta tgaacacgaa aaacgcgact tagccagccg tattgtttcc acccagcatc
    51361 atcaccacga cctctccgtc gccacgctac atgtgcacat caaccacgac gactgtctgg
    51421 aaatcgccgt gttgaaaggt gacatgggtg acgtgcagca ttttgccgat gacgttatcg
    51481 cccagcgcgg cgtgcggcac gggcatttgc agtgcctgcc gaaggaagat tgagttattt
    51541 ctaaaacaag gaaagaaaag atattaactg aacttaattt ataaaattaa gttcagttgc
    51601 atagatagat cctgtaaagg cagatattct gcgtaatgta cgtcgctaaa atttataaat
    51661 gattcaaacg ctcattcagt gtttcaataa tataggtatt aacagagact tgttgctgcg
    51721 cagccgcatt attaaggcgt tcactcagcg attctggata gcgtaacgta aaggtcttaa
    51781 tcttttctgt acgcgcatac ggctcaatac ccgcagcttt acaatcctca agatactctc
    51841 gcagggaaag ctctccttct ttttgcagcc cctgaatact gtcagagaca aaatcacaat
    51901 aacctgacaa gcccaggaat ttgccacgaa atgcattcag ttctggcacg taggtaataa
    51961 cagctggttg cccggcaatc tccatagagt ttggcgtctt cagtttgatc atggttttac
    52021 ccctatactt agtaaccagt cacacacgct ttctacagcg cctttatcgg tatctggcga
    52081 cggatgaggc cgatggaaac acgcaacgct catatttagt atgaacttgc aacgcgaacc
    52141 tcttccttcc ttaatttctc cgcctaatgc tttaaccagt gactctatat cagaccactt
    52201 gatcccttga ggaacaggag ttttaaatat ttgatccaat gtattttttt gtttcttccg
    52261 taaagcctgt accttggttc tcatggcatg tccttatgac ttcactttat gacatcacag
    52321 tatgaagtca agtttccctt ccttacgcca ttgtcccaat cgtcttcctg aatcgcagca
    52381 gtgcgatggt gaaaaacgcg ccgccaattg ccatcagcgt caggaactgc ggccagacga
    52441 tttcgaatcc ggcaccccgg tagaggatgg cctgcgcgag gctaacaaag tgtgtcgtcg
    52501 gcatggtcag cataatgtcc tgcaccatct gcggcatact ttcgcgcggc gtggatccac
    52561 cggaaagcat ttgcagcggc agcagcacca gaatcaccag cagccccagt tgcggcattg
    52621 aacgcgctat cgtccccata aaaatgccga ttgacgtggt ggcaaacaga ctgagcgcca
    52681 cgcccagcat aaacagcggg atcgagcctt caatcggtac gcccagtaca cctttcacca
    52741 tcagcaccag cgataatccc gataccacca gcaccaccag ccccatcgac cagatcttcg
    52801 ccatcatgat ctcaaacggc gttatcggca tcaccagtaa atgttccacc gtgccgtgtt
    52861 cacgctcgcg gatcagcgcc gatccggtca atacaatcgc cagcatggta atgttgttga
    52921 tgatcgccat caccccgcca aaccacgcgg gatcaaggtt cgggttaaag cgcatccggg
    52981 tttccagcga taccaacggt tcgctgttat cacggtagcg cgcgacaaag ctgttcactt
    53041 caccgttgat aatattctgg atatacccat tgccggtaaa tgcctggctc atgcgcgtgg
    53101 catcgacgtt cacctgaata tccggctggc gtccggcgag gacatcacgc tgaaaattag
    53161 gcggaatatt tatcgcgaag gtatagcgtc cggcgtccag tccggcatcc atctcatcgg
    53221 cggtgatcat ctccggtggc aaaaaccacg gacgatagaa gctgttaacg atccggttcg
    53281 ataactgcga ttgatccata tcggcaatgg cgatcggcgc gaggttcaac gatcctggcg
    53341 taacggtcgc tgacgaatac accgacaccg taaacgagaa gacaatcagc gtcagcatcg
    53401 ctttatcacc gagcagactg cgcaactctt tgatacccag attaaaaata ttgcgtaaat
    53461 ggcgcatcat ccctcctgtt ttttcagcag caggatgctt aagcccatca ccagcgggat
    53521 ggctatcagc aacgggataa aaagttgcca caaatcagtc agatccagcg ctttcgagaa
    53581 cgttccgcgg gcgatagtca gaaaatgact ggtcgggtaa acctcgccga tccaacgtcc
    53641 aggcccttcc agcgaagcta ccggatcgat catcccggaa aactgtgtcg ccgggatcaa
    53701 tgtgataatc gccgttccga aaatggcggc aatctggctt ttcataaagg tggagatcag
    53761 cagccccatt ccagtggcaa tgatgatata cagcagcgcc gccaaggtga gcgtcaggaa
    53821 actgccttta tgcggtacgc caaacacaaa caccgacagg ccgcagagca ggaaaaagtt
    53881 cagcatcccc agcgcgatgt acggcagctg tttgccaagc aaaaattcgc tacgggtggt
    53941 gggggtcacg taaaggttga taatcgaccc tagctctttt tcccgcacca cgctaagggc
    54001 gcttagcatt gacgggatca tcatcagcag aagcgggatc accgccggaa caatcgctgg
    54061 caggcttttt acgtccgggt tatagcgata acgcgtctca atgttcatca gcccgctttg
    54121 gctggcgggt gtcgattgtc ggctcgccac atcctgaagc caactctggt gcattgcctg
    54181 cacgtaacct tttaccgttt cggcacgact gggcatcgct ccgtcgatcc agacgccgag
    54241 ttccacaggc gtaccacgcg cgatatcgcg cccgaaattg ggcgggatct caatggccac
    54301 cgtgatatcg cccgcacgca tccgacgatc aagctcgtca taactggtga gcggcggctg
    54361 ttcgataaag taacgggaac cggagaggtt gagtgtccac gcctggctac tgacggtctg
    54421 gtcgcggtcg agcaccgcaa agcgcaggtt ttccacatcc atactgatgc cgtaacccat
    54481 tatcagcatc aggatcaccg ttcccatcag cgccagcgtc gaacgtactg gatcgcgtcg
    54541 cagttccagc gcttcgcggc ggctgtagct aaacagacgg cgcaggctaa atccctgacg
    54601 cggcgcgtgg gtggtgtcgt gtaccacggg cggcgcttcg gcttcgttgc tctgccctgc
    54661 cgcttcctgc aaataggcga taaatgcctc ttccagactg gcggctccgc gtttctcaac
    54721 cagttcctgc ggtgtaccgc tggcaagcac ttttccggcg tgcatcagtg agatgcggtc
    54781 gcaacgttcc gcttcgttca taaagtgggt ggagatgaag atagtcactt tgtcctggcg
    54841 cgagagatcg accatcaact gccagaacat atccctcgcc accggatcga caccagaagt
    54901 aggctcatcg aggattaaca tctccgggcg atgaatcacc gccaccgcca gcgaaagccg
    54961 ctggcgaatg ccgagcggca atgactccgg cagaacatct tcaacgtcgt tgagcttaaa
    55021 acgctcgctc atttcagcca ctcgcgcggg aatttccgct tccgggatgt gaaacaaacg
    55081 ggcatgtaac tccaggtttt gccgcacggt gagttcgtta tagagcgaaa acgcctgcga
    55141 catatagccc acacgacggc gggtatcgat atcttttgga tcaaccggtt gcccaaatag
    55201 ccacgcctca ccttcgctgg cgggcagcag tccggtaagc attttcatgg tggtggattt
    55261 accgcagccg ttcgaaccaa ggaatccaaa aatctccccg cgtggaatgc ggaaattgac
    55321 gtgatcaacg gcaacgaagg aaccaaaacg catggtcaga tcgcgcgctt cgatggcaat
    55381 ctctgcgttt tcaggttgat acggtgggat cactaccgcc tgatgcgcct ggcgttgcgc
    55441 ttgcggtaac agattaataa atgcttcttc cagcgtggcg ctttgcgttt gctgccgcag
    55501 ctcttcggcg ctgccggttg ccagcacttc tccggcattc atcgctacca gccagtcgaa
    55561 gcgttcggcc tcttccatat aggcggtggc gaccagcacg ctcatattgc tctgccgctg
    55621 gcgaatactg tcgatcagat cccagaactg ggcgcgggag agcgggtcaa cccccgttgt
    55681 tggctcatca aggatcaaca gttccgggtc gtggattaac gcgcagcaca gcccaagttt
    55741 ttgcttcatc ccgccggaga gtttacctgc cggacgatcg cgaaacggtg ctaacccggt
    55801 gctggtcagc agctcattaa ttcgcgcttc ccgctccgct ttatcgtgac cgaacaggcg
    55861 agcgaaaaaa tcgacatttt catacaccga caaggtgtgg tagaggtttt tgcccagccc
    55921 ctgcggcatc caggcgatgc gcgggcagac gtctcggcga tgcttcgggt cgtgcatatc
    55981 gccacccagc accaccacat tgccctgctc aatgacgcgg gcaccggaaa tcagtgacaa
    56041 taaactcgac ttcccgacgc catccggacc aatcagcccg accatgcaac gggccggaat
    56101 atcgagagtg atattgttca gcgcaacggt ttttccataa tgctggctca cgcccgccag
    56161 ttgcgcgaca ggcgggacgg gaaccagttc cagatgcgtc attgcggcaa cctcaccacg
    56221 aggtcgtcag gccacggaag ttcttcattc acccgcaccc acgctacgcc cggtaaaccg
    56281 gttttgacat attccagatg ctgctggagt aattccggtg ggatacgcgc tttgacgcgg
    56341 aacatcagtt tcagccgttc atcgctggtt tcgacggttt ttggcgtgaa ctgggcgaca
    56401 ctggcgacaa aactgatggt tgcaggaata cgcagatctg gcgcggcatc gaggatcagc
    56461 cgggcttcac cgcccagttt cagcgtgccc gcctgttcgg ttggcaggaa gaaggtcata
    56521 tagacgtcgc tgagatcgac catattcagc acccgaccgc ctgccgccag cacttcccct
    56581 ggctcggcaa cccgatactg cacgcgtccg tcacgcgggg ctttcagttc actgtcatcg
    56641 atatctgcgg caatgcgccg ttcagtggct tgcgctgctt cgacccgagt ttgcgcctga
    56701 atgatattgg tgcgtgctgc ttctatagcc gctttagaag ccgaaacctg ggctttcgct
    56761 gattccagcg cagctcgggc gctctcagcg gcggcgcggt cgtcatccag ctgttgcgca
    56821 gaaatagccc ctcgttgggc gagcgagcgg gaacgcgtat gacgtttggc aacagagtcc
    56881 agttcggcct ggcgttgatt gaccagcgac tgtgcggcac gagtttcact ttgtcgttgc
    56941 tccagcaaag cctgcgcggc ggcaacggcg ctttgtgcct ctttgatttg cgcgatggct
    57001 tccagtcgct gttcctgcaa cacgcgagta tccatcttcg ccagcacttc gccttcgcga
    57061 acaaactggc cttctttcac cagaatggtg tcgatacgcc cggcaatttt gctggcgata
    57121 tccacttccg tcgcttcaat gcgcccattg ctgacagcaa agccttccgg cacacccgcc
    57181 gggcgcaaca gccaccacgc cacgacggca gccaccgcca gtgccccgac aacccaccac
    57241 gccagatggc gcttactctt atccataatc gacccgccat aatccctgta agcaaacgac
    57301 gcgtgttaca actgccgccg aacgacgttc agcggattct tccatccggg ataagaagga
    57361 aacgacaaat acgaccagcg cgcagagcat tgagcgccaa cggggaaggc ttagtgaaaa
    57421 cgggtagtaa tgttgtcatc atcagcattc ctggccgtaa atgaaacacg catactcata
    57481 agtgtgatct ataaaactgg ctgacaaaac ggtatccatc ctgttggatc gcatttcacg
    57541 cctgttcagt tgcaggaaca gagcattcct ttttacgttt tatcaacagt ctcaaccaga
    57601 tagtaacatt aatattcatg ttgtctatgg ttcagagctg taacattgtg cgccttctaa
    57661 tcaaaggcat aagtccattt ctgttttttt catgctggta agacttccag ggagtggtta
    57721 tctctggccc tgcaccaggg ttaatttggc aattatgcat gctgaacatt cttccagcat
    57781 gtttactgtc acgggcgtta agacctaaaa ctgtccccgt ttcacctatg cgttctctcc
    57841 caccatttcc gttatgtgca acaacttgat ttatacctaa catttgtgaa catgctaata
    57901 gtgcttcaag tttaggtctg taatctgtcg aaacagaaaa ataacgatta aaggatcgac
    57961 aagagatagc ccggctagtt ttaaactttt tgccattaat tatagcatga tgctttttat
    58021 taataatttc cgctaattcg accggatcga gagcattggt catttcatca atagagacag
    58081 ttatcggtcc tataatgtaa cgttttcgct gatcatcaaa agttatccca tgatgatttg
    58141 ccattatttt gtatttgggg tcatagaaac atacaggata atcttttatc ccgtcgtagg
    58201 tatcctcatc aagtggtttc atgcgcgtga agttttgcat atagttacca ttgcaattcg
    58261 tttcatgatt tccagctaat acatgaacat tttcattgat actcattatg cgttttaaca
    58321 aatcgatgat aaattgatcg ccacagatat tactaaatcg atcgccggtt tggtcgccaa
    58381 gaaacgtaca tggcgtgact gcgtctttat taataaccaa ataaggctga agactttcta
    58441 aaacattatc tcttctgttc agtatactga ttattgtatt aacattagtg tcaagttgct
    58501 cattaaagtt ctcccttagc aatacgttga gaacttcatc ttctatatta agcaactggc
    58561 taaggaaatt ttctccttca gccatgattc ccaaatgtcc tgttgcaaaa agcgcgtaca
    58621 aaactgcacc gactgagcca tcggtatcgc caaaataggt cggcctggta cttgtgggat
    58681 caacaggtga acaattggag aaataggttc tgtcagcgaa ctggggtttt ataattgttt
    58741 cgaatttaat atcaaataca ttgtattgcg atatttccca gttccagatg ggtttgtacg
    58801 actcagtaac tgcaacatca ttagcattat catttactgt catattatta agtaaataaa
    58861 caatgctgca ccaattaata tcggtattat caagagggca accgtaattt ttactattaa
    58921 ttgtcacaat aaactgataa cagttattcc tacgtagaat atgagcgcgc atgcccgata
    58981 gcatactata tttttctgaa tgattcgttt gtatggtaaa tgaaattttg tcatcttgaa
    59041 tagattgttg gaaacgttct atttgccaat catcaatttt ttccacaact tcaccgagca
    59101 ttgtcctggc aatacttgat atatcatcac ctataccatt tgcaatatac ccaaacagtt
    59161 ctttagtgaa gttggtaaca tgctcttgca ccatatcatt tgttgcgatt gtcgttgtac
    59221 tgccattagt gcctgccaca gttccaattt tcatctttta cctcatatta ttcaccgcga
    59281 ggaatacata gttaacattt tatcttatga catacacctc atattcgatg gggaaataat
    59341 tatattttgc acaaatcatt cagccttaaa gcataatatg aaatttctta tctcatagca
    59401 gagaaagtcc ctaaatattg tagaggcgtc atcagtacat acctctacaa tcgattaaga
    59461 aaacctatga aatactggca aaattaaaaa gagataccac agcactcatg aagttttatt
    59521 gaggttgaaa acaactaagc cctgggacac cttttttatc accacgttcg ttaggtgcta
    59581 tcttccaaga atttagcccc atggtataat tatcaatttt ttgatgctgg tctaccccta
    59641 atccataatg gccaaatttc tgtttagttt ttttgagcgt ctgtccacat ttgaagatat
    59701 cttcaggttt tggtctgaaa gcagtagtgt tattaaaata attttcatat acaggtgtgg
    59761 gtgctatagc attgcttcta attagatgga gaagatgatc atccatgtgt tgtttatgtt
    59821 ttttattaca tatatttgct aattcctcag gatcaattgg gtctttcatc tgattaaatg
    59881 gaacctgcaa agcaccgaga caataacatt gattttcttc atttcttatt attccgtgat
    59941 gactggttaa aactttagtt tctgagttat aattacaaac atcgagcgtt ttaattaagt
    60001 tatacgtatc accggcagag agtttataat tttctagtcg tgcagtatag ttaccattaa
    60061 aattgatttc atgatttcca gccagaacga tgacattttt attaatacgg ctatctttat
    60121 taccctccat atttcgcata gaatttagca aagtaaggat atatttatca ccaaaaatag
    60181 tactaaatct atcgcctgta tgatccccaa ggaagataca aggggttatc gcatcgtcct
    60241 ttaaaaaaac atgttgatgc aactcattca atatcatgtc tctcgtattt aataaattgt
    60301 agagcaattt attattttta tgtatgtatg cagatgaagc catgtcttcg tgttttaata
    60361 attcacataa taaactccaa cccttttccc tgatgcctat atgccctgaa acgagcaatg
    60421 aataaagtac agcgcctact cttccatctg tatcgccaaa ataagccgtg ttggcatttt
    60481 gtgggttaat cacaccacat tgtttataaa agtctatttt agaaaacaca gatttaacat
    60541 agctatcaaa cttatcctta ggaaacgtct ctttagagac atcccaacgc cagtcagctt
    60601 taaaattacc gctaagaaga gtaaagaaat tttcaggtat gatagaaagc ccattaactt
    60661 tataaattat gtctgattta tctccggaga aattaataaa ataataacca tccttacact
    60721 caatcttaac tgtaatggga gacgataatt ttatcacatg gttattgtct ggtataattg
    60781 taaagataaa cgtgttagta gtattatcgg caaaactatt atattcttga atatctatag
    60841 atggctcact attgtaaaac catattttct ccatctgaag actatgttca tttaccctcc
    60901 agtgaccatg ttgcaggaaa tagatgatat gcatgccaaa atcgttatct atttgcgaat
    60961 cattatccag cttatcaatt tcaactatct ttgaacgatg tgttttatca accttgctat
    61021 ggtgttgatg attattaact ttatctgaat gcttatcttt atggtttgaa gggatgtact
    61081 taaatatatt gtcaattgct ttcataaata acccgcgata tttttgacct gttagtaaaa
    61141 aataaatgat acatgtctga aagacatatt tacgacgata taaaacaata atacttatat
    61201 gttcgctgat tttaagcacc gagactatcc ggtcttttac taaataatgg aaaacaatcc
    61261 atccctggaa gacaattgta aaaaaaaaca acagaataag aattaatctt aggataaata
    61321 tttatttatc atggcctttt gaccgctaaa aaatggcact cggcacagta aaccccgcta
    61381 tagttatgct atgcatttgt ctttaatgat atctcgtcgt tatattaaca ggctgaaatt
    61441 atcattaatg cacatttcaa tattagcagg gatacctgag gagtttaaag tgaacatata
    61501 tatcgggtag cttttcaaat taatcccttt gattataggc ttaatttgca tcgcgttagg
    61561 tggctttgtg ctggaaagtt cagggcaacg tcacgggtca tgtgctgatt tctctggcgg
    61621 ccatatgcct ggcattattc actaccgcat ttattatcat ttcgcaactc acgcgcggcg
    61681 ttaatacgtt ttacaataca ttgttcccca ttattggcta tgcggggtca attatcacca
    61741 tgatatgggg ttgggcactg ttagcaggta atgatgtgat ggcagacgag tttgttgccg
    61801 gccatgttat ttttggcgtt ggtatgattg ccgcatgtgt atcgacggtg gcagcgtcgt
    61861 ccggtcactt tctgctcatt cccaaaaatg cagcggggag caagagcgac ggaacaccgg
    61921 tacaggctta ttcttcatta atcggtaatt gcctcattgc cgttcccgtt ttactcaccc
    61981 tgctcggttt catttggtct attacgctgt tacatagtgc cgacataact ccgcattatg
    62041 tcgccttggg ttaaccgcaa tctgtgcctg tctaattggc ctagtggcca caatcgtcca
    62101 tcaaacacgt aatacgttct catccaaaga gcactggctg tggtgttatt gggttatttt
    62161 tctcggctca gtcacggtgc tgcaggggat atacgtctta gtcagttccg atgcaagcgc
    62221 ccgactggct cccggcatta ttctcatttg cctcggaatg atctgttaca gcatattctc
    62281 aaaagtctgg ctactggcac tggtctggag acgtacctgt tcgttagcca acagaatacc
    62341 gatgataccg gtatttacct gtctgttttg cttgtttttg tcatcattcc tcgcggaaat
    62401 cgcacaaact gagctggact actttattcc atctcgggtt ctggttggat taggcgcagt
    62461 atgttttaca ttgttctcta ttgtttcaat attagaggcc ggttctgcga aaaaaaatta
    62521 aaatttacat aatcccagcg ttattcatat cttaacgctg gttttttacg aacttagtga
    62581 aataatgtag aatataaaat tataatttta tattggagca catgtgttaa tgatcaaccc
    62641 cgggatacta aaaatataga cattattaaa cttgcttagc ctcgcattgc ctttacctgt
    62701 taaagatgca tcgagaaaat tgagtctgaa gttctcatcc tttatctgat tttccagtcc
    62761 cgaaactttg gccttagcat tacctgagat ccgtccgtta ctatctattt tgtaggtaaa
    62821 aaaatacgaa tgggaaaatt tcctcggtgt atcttcatca gttaagccaa caatcgtaaa
    62881 actaccgcta ccatcttttt ttaaaatgac tgaaaggtta gcagagtagt ataaagaatg
    62941 atctgtttta gcatttcttg ttaacgtcga cgtacattca agagacgttg tgctaccatc
    63001 acgagccttc cagagagcaa aaaaacacac ctgtagcaat gtgaaaacaa gtattaatat
    63061 aaaatacatt ttcttattag tcatataata gcacctctga acaaattgca taagtttcgt
    63121 cattagagga ttttatacat cttgataaat accgctgccc ttccccaagc aaataatgca
    63181 tactatttac atcatatata aaatttgcac ctggcacaca gggtaaatgc tttttcgaaa
    63241 tttcagcggc ctctatttga gatttagcga ggccaatctc gttaagattc tctgaaagat
    63301 ataaatcaca ggtgtcaatc ttacctgatt taaccaatgg ataatccgtt atactgtagg
    63361 tatctttaaa agccacggca aacatttcaa taataacaat tactaccaca agcgaaatca
    63421 aaatgcgaaa aagaggaata ctccttttgc gctctacttt ttcaacaaca tcttcttgta
    63481 aatgagaatc atcataagat tgtttactta ttgttatgtt ttcactgatg taaaagccta
    63541 ctttgggtat cgtaagaatc acattttctt caacaccaat atttttaaaa cttttgcgta
    63601 acagggatat atattggttc aatgtgttgt ttgacagatc aaatgaaaat ctctccaaaa
    63661 cgctatcaat gattgagttc ctgtcaaccg ctccaccatt actttccagt aacactgcca
    63721 aaatacaccc cgccgtagct ggaaaattta tctcatcctg ggtatttaca ttcgagagcg
    63781 agcatttgtt cggatcaaaa ataatcgcgt cattgataat gaaaaaacat ctccgctcca
    63841 tgcaggcaac aacacaaaat aagattttgt cattttagcc tattcctggt ggaactgaca
    63901 caagaaacta aaatacaaat ataaccctga aaaagtcata taaaacttat atccacagaa
    63961 taaaaaatca aacatgtcag ttttatcaat ataaagtatc gcaaggcgaa aagtgatgca
    64021 agaatactcc taagtaaatg aaagcgcacc acatgaaatt atcaacacat gtaatttcat
    64081 cattaggtgc tatcgaaaat aactcaggag ctacttaaga tgaataatgt cttactcatg
    64141 agacccgatg actcagaatt tatgatacct gatgactggt cagggctaaa cgcaaaaggc
    64201 attacatacg aacaagataa tagagtgaca gagtcttttc tgccttttaa atctttttat
    64261 tgttcaccgg tgtgcacgac agttgaactc atctcacaaa ataattgttt tgctatgggt
    64321 atccgctacc ttctggaaaa gaaaagagac ttaaggctaa atcactttaa agccttctct
    64381 cataaatgcg agacaaaacc tgacgtgtta attatgaccg ggtgcaccgt cgctgatact
    64441 tatagtatat tctcacgcat tgttgaagaa aatataatga aaacgatgac acctatcgtc
    64501 atcattactg ggcatcactc agaagatctt tgttctctgg catcgaaact gggaggagtt
    64561 aaaataatat caccgacaga aaagattgca aaaatagaaa acgctatcaa tgatgctttt
    64621 aacagcaagc ctaaaaaaat aagattttta acagatatac tctctcctca acaaattgag
    64681 accatgaaaa tggttgcgca gggtagatcc gttaatgaga tttcagccgc aatgaatatt
    64741 agcatcaaga ccgtgtatgc acataaagtt cgtgctatca ataagctggg aattaagaaa
    64801 aaagtttatg aagcgctttt ttataaagcg ctaattcagt tcaactgatg taaaaatcac
    64861 cctcgcttaa taaataatat attctatgtc tttttttata agttaaaaaa ggcgggatat
    64921 caaatcccgc catgaaatta actaatcatc tgcattattc tgcgtagata taagcctggg
    64981 tacggatggt gtaagagtct ggctcaacaa cctgttcttt catatgggaa acgacgcgat
    65041 aggcaccacc tgttccagta tttttcagca tcatataacg cttaccgcca atctcttcgg
    65101 aggcagctga aggaggatcc tgctcaagcg ttgtcaggga aactttcagt ttatggtccg
    65161 ggttattgtc accagaaatt actgcatccg ctgaagtact gctctccgca taaatatcat
    65221 ccgggaaagt tacagcatag tcgtggctct gcccatcaga tgcgccaaca atacctgttg
    65281 ccaaaggcgt ccatgcagtt aacgtaccgc tatatacccc tttcactggt tgaatggtat
    65341 tattaacaac gataggcttg gtcaaagtaa gattagctga cgcttcagta ccctggctta
    65401 ctaaagttcc cgcctgagaa gcaaaagctg tccccgccag catgattgct aacaatgttt
    65461 tcttcattat attttctcca tgattttcat ttacactgca aaatgcagtt tctttctttg
    65521 catttacgtg ctatcgcgaa aatgcttcca ccaaataatg gaaacatctt cacaaaaata
    65581 aattatgagt ttcttaattt agaaactata aacatgtcgc cgctatctgg tataaccctg
    65641 atgcactttt cttcgcagga agttggtatt ttgcgataca ggattgatct ttagaataac
    65701 cccatttcac attaaactcg ccttccgcac tcaagccaga aacataagca ataccatctt
    65761 cacctacaat gccggccata ttttcagtgt ctttgctata actaatcaca gcgccgaagg
    65821 ggatgggttt accctctttc tgttttaagg ttactaatcc tttatccccg ct
//
LOCUS       DAETQL010000011        64196 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.011, whole genome shotgun sequence.
ACCESSION   DAETQL010000011 DAETQL010000000
VERSION     DAETQL010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64196
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.011"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            133..576
                     /locus_tag="K0W65_000740"
     CDS             133..576
                     /locus_tag="K0W65_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI3094129.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            812..1630
                     /gene="ybhA"
                     /locus_tag="K0W65_000741"
     CDS             812..1630
                     /gene="ybhA"
                     /locus_tag="K0W65_000741"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HBI3094130.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1631..2689)
                     /gene="modC"
                     /locus_tag="K0W65_000742"
     CDS             complement(1631..2689)
                     /gene="modC"
                     /locus_tag="K0W65_000742"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBI3094131.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2692..3381)
                     /gene="modB"
                     /locus_tag="K0W65_000743"
     CDS             complement(2692..3381)
                     /gene="modB"
                     /locus_tag="K0W65_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBI3094132.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3381..4154)
                     /gene="modA"
                     /locus_tag="K0W65_000744"
     CDS             complement(3381..4154)
                     /gene="modA"
                     /locus_tag="K0W65_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3094133.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="K0W65_000745"
     CDS             complement(4321..4470)
                     /gene="acrZ"
                     /locus_tag="K0W65_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HBI3094134.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4599..5387
                     /gene="modE"
                     /locus_tag="K0W65_000746"
     CDS             4599..5387
                     /gene="modE"
                     /locus_tag="K0W65_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBI3094135.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5455..6927
                     /gene="modF"
                     /locus_tag="K0W65_000747"
     CDS             5455..6927
                     /gene="modF"
                     /locus_tag="K0W65_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBI3094136.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7188..8204
                     /gene="galE"
                     /locus_tag="K0W65_000748"
     CDS             7188..8204
                     /gene="galE"
                     /locus_tag="K0W65_000748"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBI3094137.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8214..9260
                     /gene="galT"
                     /locus_tag="K0W65_000749"
     CDS             8214..9260
                     /gene="galT"
                     /locus_tag="K0W65_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBI3094138.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9264..10412
                     /gene="galK"
                     /locus_tag="K0W65_000750"
     CDS             9264..10412
                     /gene="galK"
                     /locus_tag="K0W65_000750"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBI3094139.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10406..11446
                     /gene="galM"
                     /locus_tag="K0W65_000751"
     CDS             10406..11446
                     /gene="galM"
                     /locus_tag="K0W65_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBI3094140.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11649..12401
                     /gene="gpmA"
                     /locus_tag="K0W65_000752"
     CDS             11649..12401
                     /gene="gpmA"
                     /locus_tag="K0W65_000752"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBI3094141.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="K0W65_000753"
     CDS             complement(12568..13620)
                     /gene="aroG"
                     /locus_tag="K0W65_000753"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBI3094142.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13936..14316
                     /locus_tag="K0W65_000754"
     CDS             13936..14316
                     /locus_tag="K0W65_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HBI3094143.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14430..15371
                     /gene="zitB"
                     /locus_tag="K0W65_000755"
     CDS             14430..15371
                     /gene="zitB"
                     /locus_tag="K0W65_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBI3094144.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="K0W65_000756"
     CDS             complement(15368..16087)
                     /gene="pnuC"
                     /locus_tag="K0W65_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBI3094145.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="K0W65_000757"
     CDS             complement(16125..17168)
                     /gene="nadA"
                     /locus_tag="K0W65_000757"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBI3094146.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDAFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17417..17492)
                     /locus_tag="K0W65_000758"
     tRNA            complement(17417..17492)
                     /locus_tag="K0W65_000758"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17457..17459),aa:Lys,seq:ttt)
     gene            complement(17496..17571)
                     /locus_tag="K0W65_000759"
     tRNA            complement(17496..17571)
                     /locus_tag="K0W65_000759"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17536..17538),aa:Val,seq:tac)
     gene            complement(17623..17698)
                     /locus_tag="K0W65_000760"
     tRNA            complement(17623..17698)
                     /locus_tag="K0W65_000760"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17663..17665),aa:Lys,seq:ttt)
     gene            complement(17701..17776)
                     /locus_tag="K0W65_000761"
     tRNA            complement(17701..17776)
                     /locus_tag="K0W65_000761"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17741..17743),aa:Val,seq:tac)
     gene            complement(17912..17987)
                     /locus_tag="K0W65_000762"
     tRNA            complement(17912..17987)
                     /locus_tag="K0W65_000762"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17952..17954),aa:Lys,seq:ttt)
     gene            complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="K0W65_000763"
     CDS             complement(18152..18943)
                     /gene="cpoB"
                     /locus_tag="K0W65_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBI3094147.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18953..19474)
                     /gene="pal"
                     /locus_tag="K0W65_000764"
     CDS             complement(18953..19474)
                     /gene="pal"
                     /locus_tag="K0W65_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBI3094148.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="K0W65_000765"
     CDS             complement(19509..20801)
                     /gene="tolB"
                     /locus_tag="K0W65_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBI3094149.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="K0W65_000766"
     CDS             complement(20934..22175)
                     /gene="tolA"
                     /locus_tag="K0W65_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBI3094150.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="K0W65_000767"
     CDS             complement(22240..22668)
                     /gene="tolR"
                     /locus_tag="K0W65_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBI3094151.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="K0W65_000768"
     CDS             complement(22672..23364)
                     /gene="tolQ"
                     /locus_tag="K0W65_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBI3094152.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="K0W65_000769"
     CDS             complement(23361..23765)
                     /gene="ybgC"
                     /locus_tag="K0W65_000769"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBI3094153.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="K0W65_000770"
     CDS             complement(23915..24208)
                     /gene="ybgE"
                     /locus_tag="K0W65_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBI3094154.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="K0W65_000771"
     CDS             complement(24208..24321)
                     /gene="cydX"
                     /locus_tag="K0W65_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBI3094155.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="K0W65_000772"
     CDS             complement(24336..25475)
                     /gene="cydB"
                     /locus_tag="K0W65_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBI3094156.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="K0W65_000773"
     CDS             complement(25491..27059)
                     /gene="cydA"
                     /locus_tag="K0W65_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBI3094157.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27908..30541)
                     /gene="mngB"
                     /locus_tag="K0W65_000774"
     CDS             complement(27908..30541)
                     /gene="mngB"
                     /locus_tag="K0W65_000774"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBI3094158.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30559..32475)
                     /gene="mngA"
                     /locus_tag="K0W65_000775"
     CDS             complement(30559..32475)
                     /gene="mngA"
                     /locus_tag="K0W65_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBI3094159.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32645..33367
                     /gene="mngR"
                     /locus_tag="K0W65_000776"
     CDS             32645..33367
                     /gene="mngR"
                     /locus_tag="K0W65_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HBI3094160.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33470..34339)
                     /gene="sucD"
                     /locus_tag="K0W65_000777"
     CDS             complement(33470..34339)
                     /gene="sucD"
                     /locus_tag="K0W65_000777"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBI3094161.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34339..35505)
                     /gene="sucC"
                     /locus_tag="K0W65_000778"
     CDS             complement(34339..35505)
                     /gene="sucC"
                     /locus_tag="K0W65_000778"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBI3094162.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35599..36816)
                     /gene="odhB"
                     /locus_tag="K0W65_000779"
     CDS             complement(35599..36816)
                     /gene="odhB"
                     /locus_tag="K0W65_000779"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBI3094163.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36831..39632)
                     /gene="sucA"
                     /locus_tag="K0W65_000780"
     CDS             complement(36831..39632)
                     /gene="sucA"
                     /locus_tag="K0W65_000780"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBI3094164.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39933..40649)
                     /gene="sdhB"
                     /locus_tag="K0W65_000781"
     CDS             complement(39933..40649)
                     /gene="sdhB"
                     /locus_tag="K0W65_000781"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HBI3094165.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40665..42431)
                     /gene="sdhA"
                     /locus_tag="K0W65_000782"
     CDS             complement(40665..42431)
                     /gene="sdhA"
                     /locus_tag="K0W65_000782"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBI3094166.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42431..42778)
                     /gene="sdhD"
                     /locus_tag="K0W65_000783"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42431..42778)
                     /gene="sdhD"
                     /locus_tag="K0W65_000783"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBI3094167.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42772..43176)
                     /gene="sdhC"
                     /locus_tag="K0W65_000784"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42772..43176)
                     /gene="sdhC"
                     /locus_tag="K0W65_000784"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBI3094168.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43557..43664
                     /locus_tag="K0W65_000785"
     CDS             43557..43664
                     /locus_tag="K0W65_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094169.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43870..45153
                     /gene="gltA"
                     /locus_tag="K0W65_000786"
     CDS             43870..45153
                     /gene="gltA"
                     /locus_tag="K0W65_000786"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBI3094170.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45544..46110
                     /locus_tag="K0W65_000787"
     CDS             45544..46110
                     /locus_tag="K0W65_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI3094171.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46161..48620
                     /locus_tag="K0W65_000788"
     CDS             46161..48620
                     /locus_tag="K0W65_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI3094172.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48635..49366
                     /locus_tag="K0W65_000789"
     CDS             48635..49366
                     /locus_tag="K0W65_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBI3094173.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49363..50439
                     /locus_tag="K0W65_000790"
                     /pseudo
     CDS             49363..50439
                     /locus_tag="K0W65_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50591..51637
                     /locus_tag="K0W65_000791"
     CDS             50591..51637
                     /locus_tag="K0W65_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBI3094174.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51634..52425)
                     /gene="nei"
                     /locus_tag="K0W65_000792"
     CDS             complement(51634..52425)
                     /gene="nei"
                     /locus_tag="K0W65_000792"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBI3094175.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52461..53195)
                     /gene="pxpA"
                     /locus_tag="K0W65_000793"
     CDS             complement(52461..53195)
                     /gene="pxpA"
                     /locus_tag="K0W65_000793"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBI3094176.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53185..54117)
                     /gene="pxpC"
                     /locus_tag="K0W65_000794"
                     /pseudo
     CDS             complement(53185..54117)
                     /gene="pxpC"
                     /locus_tag="K0W65_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54111..54767)
                     /gene="pxpB"
                     /locus_tag="K0W65_000795"
     CDS             complement(54111..54767)
                     /gene="pxpB"
                     /locus_tag="K0W65_000795"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBI3094177.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54790..55533)
                     /gene="ybgI"
                     /locus_tag="K0W65_000796"
     CDS             complement(54790..55533)
                     /gene="ybgI"
                     /locus_tag="K0W65_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBI3094178.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55804..57285
                     /gene="dtpD"
                     /locus_tag="K0W65_000797"
     CDS             55804..57285
                     /gene="dtpD"
                     /locus_tag="K0W65_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBI3094179.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57435..58853)
                     /gene="phrB"
                     /locus_tag="K0W65_000798"
     CDS             complement(57435..58853)
                     /gene="phrB"
                     /locus_tag="K0W65_000798"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBI3094180.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58850..59359)
                     /locus_tag="K0W65_000799"
     CDS             complement(58850..59359)
                     /locus_tag="K0W65_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBI3094181.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59488..59775
                     /locus_tag="K0W65_000800"
     CDS             59488..59775
                     /locus_tag="K0W65_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HBI3094182.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59830..60210)
                     /locus_tag="K0W65_000801"
     CDS             complement(59830..60210)
                     /locus_tag="K0W65_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094183.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60213..63599)
                     /locus_tag="K0W65_000802"
     CDS             complement(60213..63599)
                     /locus_tag="K0W65_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBI3094184.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(63618..63821)
                     /locus_tag="K0W65_000803"
                     /pseudo
     CDS             complement(63618..63821)
                     /locus_tag="K0W65_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15693 a        17279 c        15622 g        15602 t
ORIGIN      
        1 cttgccgaag aagatccagc ttctgcaacc aggccattca gaactgaagt gaagcgcagt
       61 cgactgttaa ttgacgagta tctgttaatc aggaaaatag cagacgtcca gaacgaatgg
      121 ttcggccttt gcatggatct tgcactggta acaggacagc gcaaaggaga tctggcggct
      181 atgagatggg aggatataag agatagcagg ctatacgtgg aacaacaaaa aacaggggca
      241 aaaattcgca tttctctccc caccacgatt tccagattga atttaacact tgccgatgta
      301 ctggataact tgaaaaaaat aaacggtaaa aacgaaaagt tactcggagg gaagacagcc
      361 aggacaattg cagcacagtt caggattgcc cgagacacat ccggattgaa atgggaaggt
      421 gacccaccgc catttcatga aatccgaagt ttatccggtc gcctgcactc agcagaaaaa
      481 ggcagtgatt tcactcaggc gctccttgga catcgttcat ccagcatgac ggataaatac
      541 cgtgacggac gagggaggga atggaaagac atttagaatt aatttcgaat gatttcgaaa
      601 tgccacttgt aattaattga ttataaagaa ttaattccta aagagcattt gcttcatgaa
      661 tgctcctttg catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa
      721 tgctgaatta aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac
      781 gaaaattctt aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt
      841 agacggcacc ttattgaccc cgaaaaagac cttgcttcca tcatcgatag aagccctggc
      901 ccgcgctcgc gaagcaggct atcaattaat catcgtcaca ggtcgccatc acgtcgctat
      961 tcatcctttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     1021 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     1081 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtcgatga
     1141 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     1201 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacgacgca
     1261 acaagttaac gccgtatgga agttcgccct gacgcacgat gacctgccgc aattgcagca
     1321 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     1381 ggttgatatt gcacacggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     1441 gcaaggttgg tcgatggaaa acgtcgtggc attcggcgat aactttaatg atatcagtat
     1501 gctggaagcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     1561 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     1621 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     1681 gtttgatcgc cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttaccgc
     1741 cgacttccag ctccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     1801 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     1861 cctgaatgcg gatacgtagc gcagcttgca gcggttcgcc cagcttattg acccacaaat
     1921 gctgatcgcc cagcgccagc gcggtcatcg cgtaatgcgg atgatgctca agcaccgtca
     1981 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactgcccc
     2041 acacttcctc cagcgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     2101 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcgga atgttgattt
     2161 cccgtgtcag ccgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     2221 ccagcggttc atccagcaac agcaattccg gtgctgtcag caaagcccga ccaatcgcca
     2281 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     2341 ctaaaagcgc caccagctta tcgaactgat cgaccatact tttcgacatg ccgtagcgca
     2401 gattgccacg cactttgtaa tgcgggaaca accgcgcatc ctgaaaaaca tagccaacac
     2461 gacgcttttc cggcgtcagg cagatacctt tttcggcatc atttagtacc cgcccattga
     2521 ggacaatccg ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     2581 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     2641 tggtcaggca atggttgccc aacgtctggg aaaaattcag ctccagcatg attagcgccc
     2701 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     2761 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     2821 ggtatacatg gcagaaggaa tggttcgcgt ttcgctagga atgttcgaca caaaggtgat
     2881 ggttgcacca aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     2941 cggtaaggtc agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cacctagtgt
     3001 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     3061 catcagcgga aacgacatga cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa
     3121 ggtaatacca aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac
     3181 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     3241 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     3301 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     3361 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgct
     3421 gcctgcggtc ccttcagata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     3481 acaacagcaa ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact
     3541 tttacccctt tgctggcaac tgcgtcagaa ccgtagacaa tgcccagagg cgcttcgtta
     3601 cgttcgacca gcgccagcgc cccacgaaca tcttccgctg gggccagttt cggagagagc
     3661 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     3721 tccggatcgc caaccgccag gcgaccgcca ttcagcagcg aagtccagtt agttttgctg
     3781 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     3841 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caaccgcata atccatccat
     3901 ttctgatcgg cagaaataaa cagatccgca ggggcacccg cttcaatctg acgggcgaga
     3961 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tctctttttt atactgcgta
     4021 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     4081 tctgccagtg cattgccagc aactgcgaaa gagagtgccg ccccggcaaa caagttcaac
     4141 catttacgag ccattggtaa ccccttaatg taacgttatg taggcgacaa tataacgact
     4201 aactcggaat tttcccagca gttattgcta acctttgtga ggtagataag aaaaaatatc
     4261 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc actcagggaa
     4321 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     4381 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     4441 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     4501 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     4561 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     4621 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg catttcgcta ctaaaacaca
     4681 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     4741 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     4801 caggcggtaa aggtggcgga ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     4861 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg
     4921 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     4981 gtaaccagtg gttcggtacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     5041 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     5101 cacgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     5161 gcattactca ggacgaagcg gtcgcacaaa acgccgacaa ccaattaccg ggtattatta
     5221 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     5281 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     5341 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     5401 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     5461 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     5521 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagtggaaag
     5581 tcggcgctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     5641 cagttttccc acatcactcg tctctccttc gagcaattgc aaaagctcgt cagcgacgaa
     5701 tggcaacgga ataacaccga tatgctcggc cctagcgaag attacaccgg acgcactacg
     5761 gctgagatta ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     5821 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     5881 aaaaccctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     5941 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     6001 cagtccggta ttactttggt actggtgctc aatcgcttcg ataagatccc ggaatttgtc
     6061 cagtttgctg gcgtgctggc ggattgtacg ttagcggaaa ctggcgctaa agaggaactg
     6121 ctccagcaag cactcgtcgc gcaacttgcg catagtgaac agcttgaagg tgtgcaactg
     6181 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaatc gcgcattgtg
     6241 ctgaacaatg gcgtggtttc ttataatgat cgccccattc ttaataacct tagctggcag
     6301 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gcgcgggaaa atcgacgtta
     6361 ttaagcctga ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcggc
     6421 cgacgtcgcg gcagcagcga aaccatctgg gacatcaaaa agcatatcgg ttacgtcagc
     6481 agtagtttgc atctggatta ccgggtcagc actaccgtgc gtaatgtgat tctttctggc
     6541 tattttgatt cgattggcat ttatcaggtc gtttcggatc gccagcaaaa actggtgcag
     6601 cagtggctgg atattctcgg cattgataaa cacacggctg acgctccgtt ccatagtctt
     6661 tcctggggac agcagcgttt ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     6721 cttattctcg atgaaccact acaggggctt gatccgctca atcgccaact tatccgccgt
     6781 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     6841 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     6901 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     6961 agcgcactct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     7021 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     7081 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     7141 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     7201 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     7261 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     7321 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     7381 tgaccgagat cctgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     7441 ccgtcgccga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     7501 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     7561 ccaccgttta tggcgatcag ccccaaattc catacgttga aagcttcccg accggcacac
     7621 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     7681 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     7741 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     7801 cccaagttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     7861 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     7921 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     7981 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     8041 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     8101 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     8161 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     8221 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     8281 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     8341 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     8401 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggccttga
     8461 tgtctgacac gccagatgcg ccagaaagca acgatccttt gatgcgttgc cagagcgcgc
     8521 gcggtaccag ccgggtgatc tgcttttcac cggatcacag taaaacgttg ccagaactga
     8581 gcgttgcggc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gagctgggaa
     8641 aaacgtaccc gtgggtgcag gtctttgaaa acaaaggtgc ggcgatgggc tgctctaacc
     8701 cgcatccgca cggacagatt tgggcaaata gcttcctgcc taacgaagct gagtgcgaag
     8761 accgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
     8821 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgttg
     8881 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
     8941 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
     9001 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
     9061 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
     9121 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
     9181 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
     9241 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
     9301 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
     9361 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
     9421 tgatcagctg tgcaccacgc gatgaccgta aagttcgcgt gatggcagcc gattatgaaa
     9481 atcagctcga cgagttttcc ctcaatgcgc ccattgtcgc gcatgaaaac tatcaatggg
     9541 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggtg
     9601 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
     9661 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
     9721 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
     9781 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
     9841 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
     9901 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
     9961 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg
    10021 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg tcatatcctg actgaaaacg
    10081 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    10141 tgatggccga gtctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    10201 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    10261 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    10321 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    10381 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    10441 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    10501 ctggggtgcg actttacttt ccgcccgtat tccgctttcc gatggcagcg tccgcgaggc
    10561 gctgctcggc tgtgccagtc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    10621 tattggtcgt tatgccaacc gcatcgccaa tagtcgctat acctttgacg gtgaaaccgt
    10681 gacgctttcg ccaagccagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    10741 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    10801 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    10861 cgataaccgt atctccatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    10921 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    10981 gcagattctg gcggacgaat atcttccggt tgatgaaggc ggcattccgc acgacggctt
    11041 gaaatctgtc gccggaacgt cttttgattt ccgcagcgcc aaaatcatcg ccagtgagtt
    11101 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    11161 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct
    11221 gaaggtctac accaccgctc cggctcttca attctactcc ggcaacttcc tcggcggcac
    11281 accgtcgcgg ggaaccgaac cttacgccga ctggcaaggg ctggcgttgg aaagcgagtt
    11341 tctgccggac agcccgaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    11401 cgaagagtat tccagcctga cggaatatca gtttattgct gagtaatgtt aagccctcgt
    11461 catgagggct ttatcttcat attgttcaaa tcaccagcaa acaccgacat atttgcaact
    11521 caatattcac aacaacctta cactgcgcca ctattttcgc tatggttatg cgtaagcatt
    11581 gctgttgctt cgtcacggca atataatgag aattattatc attaaaagat gatttgagga
    11641 gtaagcatat ggctgtaact aagctggttc tggttcgtca tggcgaaagt cagtggaaca
    11701 aagaaaaccg tttcaccggt tggtacgacg tggatctgtc tgagaaaggc gtaagcgaag
    11761 caaaagcagc aggtaagctg ctgaaagagg aaggttacag ctttgacttt gcttacactt
    11821 ctgtgctgaa acgcgctatc cataccctgt ggaatgtgct ggacgaactg gatcaggcat
    11881 ggctgcccgt tgagaaatcc tggaaactga acgaacgtca ctacggtgcg ttgcagggtc
    11941 tgaacaaagc ggaaactgct gaaaagtatg gcgacgagca ggtgaaacag tggcgtcgtg
    12001 gttttgcagt gactccgccg gaactgacta aagatgatga gcgttatccg ggtcacgatc
    12061 cgcgttacgc gaaactgagc gagaaagaat tgccgctgac ggaaagcctg gcgctgacca
    12121 ttgaccgcgt gatcccttac tggaatgaaa ccattctgcc gcgtatgaag agcggtgagc
    12181 gcgtaatcat cgccgcacac ggtaactccc tgcgtgcgct ggtgaaatat cttgataaca
    12241 tgagcgaaga agagattctt gagctgaata tcccgactgg cgtgccgctg gtgtatgagt
    12301 tcgacgagaa tttcaaaccg ctgaaacgct attatctggg taatgctgac gagatcgcgg
    12361 cgaaagcagc agccgttgcg aaccagggta aagcgaagta aacgtcattc gtttaaaaaa
    12421 tgagaaagcc gactgcaagt gagtcggctt ttttgtttgt cgttgctaac aatggagcac
    12481 attgcctgat gcgacgctgc gcgtcttatc aggcctgtgg tgattcatcg gatacgccac
    12541 tctgacggcg catccgacaa ttaaacctta cccgcgacgc gcttttactg cattcaccag
    12601 ttgacgtaac agagcatcgg tatcttccca gccaatgcag gcatcggtga tgctcttacc
    12661 gtaggccagc ggctccccgc tatcgaggct ctgattgcct tccaccagat ggctttccac
    12721 catcacgcca ataatggcct tttcgccacc ggcaatctgc tggcaaacgt cagcacaaac
    12781 atccatctgc tttttgaatt gtttggacga attagcatgg ctgaaatcga tcatcacctg
    12841 agctggcaga cctgctttgt tcagcccttc tttcacttca gcaacgtgct tcgcgctgta
    12901 gttaggctct ttaccgccgc gcagaatgat atggcaatcg ccgttaccac tggtattcac
    12961 aatcgccgaa tgcccccatt tagttacgga caggaagcag tgcggcgcac cggcggcatt
    13021 aatggcatcg atagccactt taatcgtacc gtctgtgcca tttttaaaac ctaccggaca
    13081 agaaagacca gacgccagtt cgcggtgcac ctgcgattcc gtggtacgtg caccaattgc
    13141 gccccagctc atcaggtcag cgagatactg aggagtgatc atatcgagaa actcacccgc
    13201 cgctggcaga ccgctgtcgt taatatcaag cagcaattta cgggctatac gcagaccgtc
    13261 gttgatctgg aagctgttat ccatatgcgg atcgttaatc aaccctttcc agcccaccgt
    13321 ggtacgcggc ttttcaaaat agacgcgcat tacgatttcc agctcatctt tcagctcttc
    13381 acgcagcgcc agcaagcgag tggcatactc tttagccgcg acaggatcat gaattgagca
    13441 cgggccaatc accaccaaca ggcgatcatc attacctttc aggatcttat ggatcgcttt
    13501 tcgggcatgg gcaaccgtat tcgcggcatt ttcagttgcg gggaattttt ccagcaatgc
    13561 gacaggagga agtaactctt tgatttcttt gatgcgtaaa tcgtcgttct gataattcat
    13621 gtctgttcca gtgttgccat acttatctta gtgaatgcaa tacttccaat ctatatcttc
    13681 cgtcagaatg tgtaaacggg gttttacact atgaacggat taatcctgga attagcaaga
    13741 aaaacgccag attgtcgcga aaaacgagat ctctcctaca atttctaact gtaactcctt
    13801 tgtttgttaa ttatttcaag attctctgct gcgtttcata aactggctga aatcttaaac
    13861 caatgcctta tattcacctg caaatgcact gttggaagag gttatccgac ataacgacca
    13921 taacaggagc atcctatgaa aatgacaaaa ctggccacat tatttctgac tgccactcta
    13981 agccttgcca gcggtgccgc actggctgcc gatagcggag cgcaaactaa taacggccag
    14041 gcaaacgccg cagctgatgc gggccaggta gcccctgacg cccgtgaaaa tgtcgcgcca
    14101 aataacgtcg acaataacgg ggtaaatacc ggttctggcg gcacaatgct gcattcggat
    14161 ggttcttcaa tgaacaatga cggaatgacc aaagatgaag agcacaagaa caccatgtgc
    14221 aaagatggtc gatgtccgga tatcaataaa aaggtacaaa ccggtgatgg catcaataat
    14281 gatgtcgata ccaaaaccga cggtaccacg cagtaaccct gcaaaatagc caataatggg
    14341 agagcggagt ctctcccttt taatttttca cttctaaaag cagtatgatg tcataaagtt
    14401 aagtgagaat aattaaggga tgactcgtca tggcgcactc acactcacac acgtcctcac
    14461 acctgccaga agataataat gctcgtcgct tgttgtatgc tttcggcgtg actgccgggt
    14521 ttatgctggt agaagtcgtt ggtggttttc tttctggttc tctggcattg ctggccgatg
    14581 cgggtcatat gttgaccgat actgccgccc tgcttttcgc cctactcgcc gtgcaatttt
    14641 cacgtcgtcc tcccaccatt cgccacactt tcggttggct aagactgacc acgctcgcgg
    14701 cgtttgtgaa tgccatcgcc ctcgtggtaa ttaccatttt gattgtctgg gaggcgatag
    14761 aacgcttccg cacgccgcgt ccggtcgagg gaggcatgat gatggcaatt gccgtggctg
    14821 ggttgctggc aaatatactt tctttctggt tacttcatca cggcagcgaa gagaaaaacc
    14881 tcaacgtacg agcagcggca ctgcatgtgc tcggggacct gctgggttcg gttggggcta
    14941 ttattgccgc gttgattatt atctggactg gctggacacc tgctgacccc attctctcga
    15001 tactggtgtc gcttctggtt ctgcgtagcg cgtggcgact gttgaaagat agcgtgaatg
    15061 aattacttga aggtgcaccg gtatcgctgg atatcgctga actgaagcgt cgtatgtgcc
    15121 gggaaatccc ggaagtccgc aatgtgcacc atgtacatgt atggatggta ggcgagaagc
    15181 cggtgatgac gttgcatgtg caggtgatcc cgccacacga tcacgatgcc ttgttggatc
    15241 agatccaaca ctacctgatg gatcactatc agattgagca caccaccatt cagatggaat
    15301 atcaaccttg tcataggcca gactgccacc tgaatgaagg ggtgtctggc cattcacatc
    15361 atcaccatta atgggacagc gcgcgtgagc ctctttcacg tgcgctgttg atccacatcc
    15421 ggctgccgtt gagcgcaata aaggtcagga tgatgtactc cagagacatg gcgtaaacgc
    15481 cctgaagtgc aaaaataacg acgctaatca cgttaataat cacccacaac agccagtttt
    15541 ccacatactt acgcgtcatc agaatcattg ccacgataga taacaccatc atgcatgaat
    15601 cccagaacgg gaaagcgtcc ggttgcagtt caggcatcac aacctgtaat cctaatgctt
    15661 gcatgatcat gactgccacg cgggtcaaaa atgcaaacac cggattgata aataccgtca
    15721 tcagaccaat cgaaacaacg caaaccgcca accagctgag tgccttcggc aatggcaacc
    15781 agcgaatttt caactccgcc tcgttctgac tggtttgtcg cgaccacgca taccagccgt
    15841 aaatattcgc ggcaaagaaa aacacctgta atagcaggct ggcatacagc tgaatctgaa
    15901 agaaaataat gccaaacaag gtaacgttga tcaggccaaa gaagtagttg ctgatcttct
    15961 ccagactggc aaggccaata cacagcaacc cggcgatcgt gcctaccgct tcgatccatg
    16021 agagatcata accgcccgcc cctattggta tatgtaccag gatattctgc acactaaaaa
    16081 aatccatctt tcccccaaag cgtaacgtta cgccttatta ttcgttatcc acgtagtgta
    16141 gccgcaaaat ccagcatacg attgagcggc accagcgccc tctctcgcag cctctcatcg
    16201 acatgaacct cgtgattact tccttcctgt tctaatgcct ctgcgatagc ctgaaggtca
    16261 ttcatggcca tccacggaca atgcgcgcag ctgcggcagg ttgcgccctc acccgcggtt
    16321 ggtgcttcca gtaactcttt atctggcacc gcctgctgca ttttgtagaa aataccccga
    16381 tcggttgcca caataagcct ctgatgaggc aatgcttttg cagcagcgat cagttgactg
    16441 gtggaaccga ccgcatccgc catatcgaca atagcttgtg gtgattctgg atgcaccagt
    16501 atggcagcat ccgggtattc ttcttgcaag cgggttaacg cctgagtctt aaatgcatca
    16561 tgcacaatgc aggcaccctg ccagcataaa atgtctccac ccgtctgttt ttgcacgtaa
    16621 cgccccagat gtttgtcggg tgcccagatg attttttcac ccaaactatc aagatgatca
    16681 ataagttcga cggcaatgct tgaagttacc acccaatctg cgcgcgcttt taccgcagca
    16741 gaagtgttgg cgtagacgac gacagtacga tcgggatggg catcgtaaaa tgcgttaaat
    16801 tcttcaacag ggcagccgag atccagtgaa cactcaacct gaagtgtcgg catcagaatt
    16861 gttttttccg gactgagaat tttggcggtt tctcccataa atctcacccc agcgactaac
    16921 aaagtagaag cgggatgctt tgcaccgaag cgcgccattt ccagagaatc agaaatacag
    16981 ccaccggttt cttctgccag ttgttgaatt tcgggatcgg tatagtagtg ggcaaccatc
    17041 accgcattac gttcttttag cagacgtttt atcttctcgc ggtaatacgc tttttcatca
    17101 atgcttaacg gcgtcggctt cggggggaaa ggataaatcg ccgtgtctgg atcaaacatt
    17161 acgctcatct taccatctcg ttttacaggc ttaacgttaa aaccgacatt agcgtaacat
    17221 tcgctgttat cggtgtaatt tgttttatat gctaaacaag atagtcgaat ggtggggaaa
    17281 agtcactcga attacgccta attccaccga cgtagagttg cttgcacagt aagctctgac
    17341 gatgtcatca ggtttacaaa gtgaaattga ggatgcctga atagcaaaaa agcgccttta
    17401 gggcgctttt ttgcattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17461 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17521 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    17581 ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat
    17641 tcgaacctgc gaccaattga ttaaaagtca actgctctac caactgagct aacgacccgg
    17701 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    17761 gctgagctaa tcacccgaca ctacgctgga tactacaact aactatgtta gtgggggtcg
    17821 tgcaggatga ctcgacttcg tctcgccctt cggcgccgtt gctgatgcaa cgttatcctt
    17881 cacgttttac taccgcttcc accttagcga ttggtgggtc gtgcaggatt cgaacctgcg
    17941 accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccact ttttcgttgc
    18001 tttcggtttg tttgatatcc cgtggcaacg gcggcatata ttactgattt cagacttgag
    18061 cgcaacaaaa atttcgatgc agatcactca actgcttatg attcgcacga cacgaccaga
    18121 aataatgcga cttctggtcg tgtgttatgc attacatcgc gttcagacgt ttttgtgcct
    18181 gtttagcgcc atcagtacca gggtatttac tgataacctg ctggtatacg gctttcgctt
    18241 ttgcggtgtc acctttgtcc tgcatgatga cgccgacttt aaacatcgca tcagcagcct
    18301 ttggtgactt cggatagttt ttcactaccg aagcaaaata gtacgccgca tcatcttttt
    18361 tacccttgtt gtagtttaac tgacccagcc aataattggc gtttggcagg taagttgaat
    18421 cagggtaatt tttgatgaaa ttctgaaatg ccaccattgc gtcatcctgg cgggatttat
    18481 cctgcaccag cgcaatagct gcattgtaat ccgtgtttgc atcgccgctt ttcaccggcg
    18541 cgccagcatt cgcagtacca gcatcagctg tcggcgtcgt tgatgccgtc gcaccgcttt
    18601 gatcgctgct ggttgattgc gccgctgcac caccgctgct gaggctgtcg atctgcaaca
    18661 ggatctgctt ttgccgctcc acgacctgat tcagttgata ctgattttcc tgaatctgac
    18721 cacgcaggga atcaatatcg gattgattat cagaaagttg ttgctggagt tgggttaaaa
    18781 gctggctgtg agcattagaa atacgctcaa gttgagtgac gcggtcttcg accgagcctg
    18841 agctgacact actgattggt gcctgagcaa aagcggccca gggggccgct ataccaacca
    18901 gtaacgacag actcaatagt tgatgtctga agttactgct catgcaattc tcttagtaaa
    18961 ccagtaccgc acgacggttt ttggagtatg ccgcttcgtc atgacccagt actgcaggtt
    19021 tttctttacc gtaagaaacg atggagatct ggtctgcaga aacgccttta ccctgcaggt
    19081 acatcttaac ggcgttcgca cgacgttcac ccagggagat gttgtattcc ggagtaccac
    19141 gttcgtccgc gtgaccttct acggtgactt tgtaagacgg gttgctacgc aggaagtttg
    19201 catgtgcatc cagcatttga gcgaagtcag aacggatatc gtacttgtcc agatcgaagt
    19261 aaacgatgtt gttctgctgc agctgttgca tttgcagacg agcctgctct tcggaagaca
    19321 tgttgccgtt gccgccgttc gcatccatac cagtgccggc acccagcatg ccttcgctgc
    19381 cgtcattgct ggcgttcttg ttggaagaac atgccgcaat tgccataaca ggcagagcaa
    19441 tcatcagccc tttcagcact ttgttcagtt gcatttcaat gattccttta ctattcaatt
    19501 aattattatc acagatacgg cgaccaggca gggaatttga cctgtccatc agttgccgga
    19561 agacgcgctt tgaaacgccc atctgtagaa accaaattca gcacggatcc catcccctga
    19621 gaagagctgt agattaccat agtgccgtta ggtgccagac ttggcgtttc atccaggaac
    19681 gtggacgaca gaacttgtac gcctcccgtt gccagatctt gtttggcaat gtgctgctgc
    19741 ccaccattgg agctgaccat taccataaat ttaccgtcgc tgctgacatc cgcatcctgg
    19801 ttctgcgaac cttcccaggt aatacgttgt ggcgcaccgc cgttgatatt cactttataa
    19861 acctgcggac gaccggcctg gtcagaagta aatgccaggt tctggctatc cgggaaccag
    19921 gtcggttcgg tattgttact gcgaccatca gtcacctggc ggatctgacc agaagccaaa
    19981 tccattacgt acaggttcag actaccggtt ttcgacaagg cgaatgccag tttgctgccg
    20041 tctggcgaga atgcaggtgc accgttgtga cgcgggaatg aagccacctg acgtacagcg
    20101 ccatttgcca gcgtctgaat aaccagcgcg gaacgaccgc tttcgaaggt cacataagcc
    20161 agtttagaac cgtctggtga ccacgccgga gacatcagcg gctgcggtga acggtgaacg
    20221 acaaactggt tgtaaccgtc atagtcagat acgcgcagtt catacgggaa ctgaccgccg
    20281 ttggtctgaa caacgtaggc aatacgggta cggaacgcac ctttaatgcc ggtcagtttt
    20341 tcaaacactt catcactggc ggtatgacca gcataacgca gccactgctt gttcactttg
    20401 tacgagttct gagcaagtac agtacccggt gcgccgccag tgtcaacaag ttgataagca
    20461 acattgtaag aaccatccgg attcggagtg acttgaccga caactacagc gtcaatgccc
    20521 agtgcggacc atgcagctgg ttgtacttcc tgcgcactac ccggctgctg tggcagacga
    20581 gcgcgatcta acggattaaa tttaccgctg ttacgcaagt cagcagcaac gatgccgcca
    20641 atatcttcag gtgccgcacc aggccccgcc cactggaaag gaacaacacc aataggacga
    20701 ccggaatcta caccgctgtc gatcacaatg cggacttcag catgcagaac tgatgcccac
    20761 agtatgagaa aaccaaatgc tactcgtaat gcctgcttca tcatatctcc cttatctgga
    20821 ccgatggccc acgataattt agcagaatgt taacaaacta aaatacacaa aactaccaga
    20881 accccgtggc aaccggtgcc tgatgttgac cgtccgaaca gtcaacatcg cgattacggt
    20941 ttgaagtcca atggcgcgtt tttgaacact tcatatactg cctggcttgg tggtttcggg
    21001 atcttcgcaa gtttagctgc tgccaacgca gcctgacaaa gtgcgggatc gccaccttca
    21061 ggtttgatat ccagtaacat accatcgggt gccagtttta tgcgcagcgt acaggttttg
    21121 cctgcatagg acgatgcgtc atagaactta ctttcgatag cagatttaat ctgcccggca
    21181 tagttattga tatcggcccc tgatgcgcca ttgtttttag tattaccact cccggcaggc
    21241 gaagcattgt tccctttcgc ccctcccccc gttttcggtg cattcttacc agagcttagc
    21301 tcaccgaaaa tatcatctgc ctctgcggca gcttttgctg cagcggcttt ttcagctgct
    21361 gcttttgccg ctgccgcttt tttgtctgcg gcggcttttt ctgccgctgc tttcttatca
    21421 gctgctgcct tatcagcagc cgctttttct gctgcttttt tgtcggctgc agctttctct
    21481 gctgccgctt tcttatcagc tgcagccttt tcagcagccg ctttcttctc agcttctgcc
    21541 ttggcttttt cagcagcttc agttgccgct ttctttcttg cttcagctgc agctgcttct
    21601 gccgcttccg ctttcttctt cagtgccgca gcggctacct cggctttttt ctgcgcttcg
    21661 gctgcggctt tggcggcttc tgcttctgct tttttcttcg cgtctgcagc cgctttcttc
    21721 gctgcttctt ccgcagcttt agcatctgcc gccgctttcg ctgccgccac ttcagcttgc
    21781 ttctgcttta actcggcctg ttttgcggct tcttcagcct gctttttctg ctcctgagcc
    21841 gctaaccgct ctttctcaag ttgcttcagg cgttcctgtt cagccgcttg tttctcacgc
    21901 agttcttcag cagcctgctg ttccttcatc ttgcgctgtt catcagaacg cttcgcgctt
    21961 gattcctggc tttgcatgcg tttgtactgc tcaactaccg cacctgaatc aaccatgaca
    22021 gcgtcgatgg acgaaccacc gccgcctccg gctgaagctt ctatattctc atcgaacgaa
    22081 ctccagatca gcgccgcaaa taagatgaca tgcagcactg ctgaaataat tatcgcccgc
    22141 ttgagcttgt cgttttgttc ggttgccttt gacactctcg gtttccaaaa actgttcgcc
    22201 tgttacccgc tctctttcaa gcaagggaaa cgcagatgtt tagataggct gcgtcattaa
    22261 accaaccgat ttcacacccg cactatgtaa caagttcagt gctttaatta tttcatcgta
    22321 aggcacatct tttgcgccac cgatcagaaa gaccgttttc gggttggcct tgaaacggct
    22381 ggacacttcc gccaccacct gctctggtgg taaacgctcc aggcgatctt tctcaaccac
    22441 cacggtgtac tgaccaatac cagacacttc aacaatcact ggcggattat cgttactgct
    22501 caccgcctgt gattcagtag cgtctggcag atcgacctcc acgctctggg tgatgatggg
    22561 cgctgtcgcc ataaagatca gcaacagcac cagcagtacg tccagcaacg gaacaatgtt
    22621 gatttcggac ttgagatcgc gacgacctcg tccacgcgct ctggccatgg cttacccctt
    22681 gttgctctcg ctaacggtaa acgcctggcg gtgcagaatc gcggtaaact cttccataaa
    22741 gttgtcgtaa ttcagttcca gtttgtttac gcgctggttg agacggttat aagccattac
    22801 cgccggaatt gcggcgaaca gaccaattgc cgtcgcaatc aacgcttctg cgataccggg
    22861 cgcaaccatt tgcagtgttg cttgttttac cgccccgagg gcgataaagg cgtgcatgat
    22921 cccccagacc gtaccaaaca gaccaatata cgggctgatg gagccaaccg taccgaggaa
    22981 cggaatgtgc gtttccagat tttcaagttc acggttcatg gagatacgca tagcacgcga
    23041 cgccccttcc actacggctt ccggcgcatg gctattggca cgatgcaggc gcacaaactc
    23101 tttgaacccg ctgtaaaaga tttgttccga accagtcaga ttatcccgtt tcccctgact
    23161 ctcttgatag aggcgagaga gttcgattcc agaccagaat ttatcttcaa acgcttcggc
    23221 ttcacgcgcc gctgcgttaa gaatacgggt tcgctggata ataatggccc aggatgcgat
    23281 tgaaaaacca atcaaaatca acatgataag tttaaccaga aggctagcct tcaggaacaa
    23341 atcaaggata ttcatgtcag tcactgctta aactccgcga caatagactt gggaagcgca
    23401 cgaggcttca ttttgagtgg gtcaacgcaa acaaccagaa cctctgcttc attcagcaaa
    23461 gtattctcgg cgttgacaat acgttgcgtg aaaaccaaag aggtgccacg cattgatgtt
    23521 atttcagtct gtatttcgag catatcgtcg agccgcgcag gtgcgtaata ttccaccgtc
    23581 attttacgta ccacaaaggc aacgcgttca gccatcagcg cctgctgact gaagtgatga
    23641 tgacgcagca tctctgtgcg tgctctttca taaaaagcga cgtaactggc gtggtacacc
    23701 acaccaccgg catcggtatc ttcatagtag acgcgaaccg gccatcgaaa cagcgttgta
    23761 ttcactttac atcccggtaa tgcaacaaaa gttagagctt ttaaacgctg ctactatacg
    23821 cgcgcgattg aggtttggga atgagggcaa gttaagggcg cgtaaaaatt tatgggccta
    23881 gagcggccca taatttttta aagagaattc tgatttaaaa gaagaagaaa atcagcccga
    23941 caatgagaac aatatcggca agcaatgggc aaaaaatccc ttgccaaaga accttctgcg
    24001 gacgaaagcc cacgccgtga atcacaccag cacagacggc ccacatcagc aataaaccat
    24061 gccagatttc cagttcactg gtcttcgcgg caaaacgaga tgggtcccaa aacatacatc
    24121 ctgctaacag aagcgccatc acgaaggaaa gcgcccgcag ggggcgcttg tccattaccg
    24181 catacaaggt cgcgataatc ttactcatca gatgtcttct tgaccggctt tgcctgattc
    24241 gacgtgttca agcgccagcg cggtgattac cccaaacgaa caggcaagaa gcgttcccag
    24301 aatccatgcg aaataccaca tttttagctc cttacttagt acagagagtg ggtgttacgt
    24361 tcaatatctt ctttggtgat acgaccgaac attttccagt aacaccaggc ggtgtagagc
    24421 agaatgatcg gtaccagaac caccgcaacc caggtcatga cgttaagcgt cagctggctg
    24481 gaagttgcat cccacattgt cagacttgcg ttcatcatgg tgctggacgg catcacaaac
    24541 gggaacattg cgataccggc tgtcaggatg atgcaggcca gcgtcaggga ggagaacaca
    24601 aacgcccacg cggctttatc catacgtgca gtcaggatgg tcagcagcag cagaaccaca
    24661 cccagtgccg gaatagccca cagaattggc gtgttgttga agttaaccag ccatgcgcca
    24721 gcttcacgaa ccacttcttt attcagtggg ttagaggctg cgtaatggtc cattgtcgat
    24781 ttcacgacat aaccatcgat accgtacatc acccatacgc cagccagtgc gaaacagacc
    24841 agtgtcacca gcgcagccac ctgagccgtt gcacgggtac gcaggtgcag ttcgcccacg
    24901 gtacgcattt gcagataggt tgcgccctga gtaatgatca tccctacgct caccacgcct
    24961 gccagcaggc cgaacgggtt aagcaactgg aagaagttac cggtgtagta cagacgcaga
    25021 tattcatcaa cgttgaacgg tacgccctgc aacaggttac cgaacgctac accaattacc
    25081 agcggcggaa cgaagctacc aatgaagatg ccccagtccc acatgttacg ccagcgggtt
    25141 tcttcaatct tggagcggta gtcaaaaccg accggacgga agaacaaaga cgccagcacg
    25201 aggatcatcg ccacatagaa gccggagaac gcagcggcat agaccatcgg ccaggcagca
    25261 aagagtgcgc cgcccgcggt gatcagccaa acctggttac cgtcccagtg tggtgcaatg
    25321 gagttaatca taattcgacg ctcggtgtcg ttacgaccga ggaaacgggt gagcatgccc
    25381 acccccatgt cgaaaccgtc agtgactgca aaaccaatca gcagaacgcc aaccagcagc
    25441 caccagataa aacgcaatac ttcataatcg atcatttgac gactcctgtc ttagcgtgcc
    25501 ggctgagtag tcgtggaaga ctgctcaaag tgatagcgac cggttttcag gctgcttggg
    25561 ccgaggcgtg caaacttgaa cattaagaac aattctgcca ccaggaacag ggtatacagg
    25621 ccgcaaatca gcaccattga gaagatgaga tcgcctgcgg tcagtgacga attcgccaca
    25681 gctgtcggca gcacttcacc gatagcccac ggttggcggc catattcagc cacgaaccag
    25741 cccgcttcta cagcaatcca cggcagcgga ataccgtaca gcgcggcgcg cagaagccat
    25801 tttttctcgc caatgcggtt gcggatgaca ctccagaaag agagcgcgat gattgccagc
    25861 agcaggaagc cacacgccac catgatacgg aacgcaaagt acagcggcgc tacacgcggg
    25921 atggagtctt tggttgcctg ttgaatctgc gcttcagtcg catcagccac gtttggcgta
    25981 tagcgtttca gcagcagacc gtaaccgagg tctttcttca tgctattgaa ctggtcacga
    26041 accgtctggt cggtagaacc agaacgcagt tgttcgagca gagagtacgc cttcatcccg
    26101 ttacgaatgc gttcttcatg ctgcaccatc agctctttca ggccgataac cggggtatcc
    26161 acggaacgcg ttgcaatgat gcccagtgcg taagggatct gaatcgcaaa tttgttcgtc
    26221 tcctcttcct gatcaggaat gccgaacaaa gtaaaggcag caggcgcagg ttgcgtttcc
    26281 cactcggctt caatagcagc cagtttggtt ttctgcacgt cgcccatttc gtagccggat
    26341 tcatcaccca gaacaataac agacagaaca gcagccatac cgaagctggc agcgatagca
    26401 aaggagcgtt tagcgaaggc gaagtcacga cctttcagca tataccatgc gctgataccg
    26461 aggatgaaca tcgcgccagt cacataacca gacgctacag tgtgaacgaa tttcacctga
    26521 gcaaccgggt taagcaccag ctcggagaag ctcaccatct ccatacgcat agtttcaaag
    26581 ttgaaatcgg acgcgattgg gttttgcatc cagccgttcg caaccagaat ccacagtgcg
    26641 gacaggtttg aaccgagcgc caccagccag gtgacacaca tatgctgaac tttacccaga
    26701 cgatcccaac cgaagaagaa cagacctaca aaggtggatt cgaggaagaa ggccatcaga
    26761 ccttcgattg ccagcggcgc accgaagata tcccctacat agtgggaata gtaagaccag
    26821 ttagtcccga actggaactc catggtcaga ccggtagcca cacccagagc gaagttgata
    26881 ccaaacaact tgccccagaa cttggtcata tctttataaa tctgtttgcc ggagaggacg
    26941 tagaccgttt ccataatggc caggaggaac gccataccga gcgtcagtgg cacaaaaagg
    27001 aagtggtaca tcgcggtcaa ggcaaactgt aagcgcgaca gttcgactat atctaacatc
    27061 atgactcctt gctcatcgca tgaagactcc gagagtgaac cccgttagaa agggtcacac
    27121 gcatgcccca atagaaattt gtttgaacac tttcatcata ccatgtgaat gcacacaaaa
    27181 acggcgtaaa gaaaggttgc aaacatgtta ataaaaactc aaattgatcc cacgtatata
    27241 ttacgccgca aaatccttac aataaacagg tttttgttga agcaaatttg catttttcga
    27301 cggtgatcaa tttatagcta aattgccgcc tttcagccaa tttgatcgag aacaatttat
    27361 ctctttttga tgcccatttc caagacttat acattgataa atatcaattt ctacaaatta
    27421 tgttaatcat gtttcaacaa ggtaacttta gtgatattta tgtttaaaat gtgtgaatta
    27481 taagactgaa attagtttcg gttggcaatt aaagataaaa agagacgaag caaaaacagc
    27541 acggttgacc ttaattcttt ttttggtcac tggctaatac gacgcagagt ttcaggatta
    27601 ttaataaagg cataaatctc ccccccgccg atattaacat ttaattaact tataattata
    27661 ttataattaa ctcacccgtg gttattattt tgttaaaaat aaagccacat atacgtagct
    27721 tgagagtgaa gataaacaca aaattacctg atgcgctacg cttgccagtg tctacatcat
    27781 ctctgcaata tattgaattt gcctgctttt gtagaccgaa taaagcgttc atgccgtatc
    27841 cggcatgaac aaagcgccct ttgtcaacaa tctggccgcg catatgtggc ttcggtttga
    27901 ttgctattca ggcaagccgg taactgaacg tccgtgactg gccgggtaaa aaaggccccg
    27961 atagatttga accttgattt tcctcggtgg taatgtgttc atccatcatc gtttctgagc
    28021 aagaaatcac ctcgcgactg aaagcaacag tcgcatcaca ggttgctgat tcagccggat
    28081 taaacagccg taaaatcact tcttgtcggt cttcagcttt cttaagtgcg cttatcaggc
    28141 atcccactgg gggcattttc aacaaactat aactttccgg cacgttgaat ccggctttgt
    28201 tgagcttcat cgcatcccat gggattttgt tgtaacactg tactggagtc agccatgctc
    28261 gcgcctgctg agctacacca gcggcggttg gcgtaccggt ataactcaat aaacttaggc
    28321 gacaagaaag cagaccacgt agttgtgagt ccgggactgg cattttaatt cccgaaggcc
    28381 gcctaggcct taaaagcaga tcttctttcc ccagtaagcc cacgccgcgc agcaacgtaa
    28441 tggcaaaggt tttcttctct tcaccgatga cttcaaattc acgtagccct tcgctaaaga
    28501 cagccatgcc gttacgccct tcctgtaagg ctgcatagtt gagcatattc cataccggaa
    28561 ccggcgcttc tttccagctt tcttgctgcc agttgttcat tgcactgtcg ttcacggggc
    28621 gcgttagcga accaaactgg gtatctgcca gaacactatc ggtgttaaaa gatgtaggga
    28681 tcaggacgcg aaggcgatga tcgtcagcct ggttatcaag gttgatatcc acatcaatgc
    28741 gcctgttgtt atgactaaga gtgacaacca actcgacatc tactctgcca gtggattgtc
    28801 ttgcgctgcg ttgttgcaga ttacgcggca ccgccatttc atagcggata acagccctgc
    28861 tctgccaggc ctcatgaata atctcgcatt gcggtttcgc attcgccgaa gtaattaccc
    28921 actcttcttt tgcgggtgaa tagtcatatt catcaccatc atcagagctt tcttcaattt
    28981 gcaacacccg gtcatagcgc acaccgctat ctttatctac cagttgtagc gttccatcct
    29041 cattgagcgc aatttgccag aaagcatttt ccagtatccc ttcagcgtca ctttttgccg
    29101 caatcacgtt gccaggctga ttcgcttcga tataaagcgt gcgatagccc atagaaggga
    29161 caatctggtt gatctgtata tcaaactcca taaagggatc gtaattaccg taatgaacta
    29221 tttgccgatc gattaggcct ggatcgatct cacgggcatg gcgaataaaa tacggtacag
    29281 gctgaccgcg atcgtctcgt agattaaact ggctggcgcg cagccgcaca gtggtgttga
    29341 tcacttcttc ccgcggccag ggcatcaggt taaacaggac gagtttgtcg gcgtcgctct
    29401 gcggcatgtt gtcggcgatt ttacgcatat agaaacgcag cagattatcc gccatgtctt
    29461 cagccagttc gaagcgggcg acgatttcgc gatgaacttt gtcacgacag cagcagccga
    29521 tgctgtcgtg ggcatgattt tttaagatct ctttccacat tttctccagc aagccgtggt
    29581 ggtatccaaa acccaacgtc ctggccagcg ttgccagcgg ttccagcaga ttaacaatct
    29641 tattttcaat acgtgcgtgg gcaactttga tatccatgcg cgtggaaccg atggtgcgat
    29701 gcacgcgcat atatttgcca tcaataaatt cccctttcag ggttgccaga ttatctcgct
    29761 gcgcttcgat cttctcaaat acctcttcaa agcggctcat cacaaacttt cgttgaggat
    29821 aaatttcgcg cagtttctcc atcacttcga agatattttg ctgcaatggc atctgatcat
    29881 gcccattcgg cagcaaaatc tctttggtta cagacgcttt ttccagcacg tcaaaataac
    29941 tgtcgaggcg tttgcgtaat ccgttttcgt ccgcaggtaa gtacttaccg atggcataac
    30001 ccaacggcat cacctgcgcc gtcacttcgc taccgtcact gctttgccac aaaaactcgg
    30061 ttttatccgt accgtggcgc tcagaacatc cacgccagaa catggtgcag gtaatgccaa
    30121 atccattgta gatatgcggc agttgcccgg acatgccaaa ggaatcgggt aaataaccta
    30181 ttttcatcgg ctcgccaaac gcgaggcagt cacgcattcc gtacatcaga ttacggacga
    30241 tggattccgc agaaacaatc gtggtatcgg tctgggtata ccaggggccg ataatcaact
    30301 tgccggcttc taccagtttc ttcaagcggt ctttgttttc cggtttcacc gcgaaataat
    30361 cttcgaggat cgccgtttgc ccgtcgagta cgtaatattt gtattcgttg tcctgttcca
    30421 gtcggcacag gatctcttcc atattattga ccagcagaat gcgtgactct tcggtggtga
    30481 aataccactc ccgatcccag tgcatatgcg gggtgatgtg aacgcgagat accgctttca
    30541 tcgtcgtttc ctttttgttt atggcattac gccatcagtc agatagttgc catgcttaac
    30601 cgcgtgacgc cgccagatca ggagaattgc agtcgagatt gcagccccca ccaatgccgc
    30661 accaaaccag ccaattgccg ccataacacc gcccgcgcca ttatcatgaa gtaaaaagag
    30721 cgagaaaatg ccagcaccgg gtgtcgaaag gccgatattc atcgcaccga caatagcgcc
    30781 cgttaccata gagcccagca caaacgaacc aataacccgc agcggatctt caatcgccat
    30841 cgggatcgcc ccttcggtaa tacctgccag ccctaacagc caggtggatt tcccggtctc
    30901 aatttcaaac tctttaaaca ggcgcggtgc gagcatcgtg gaagcggtta cggtaaatgc
    30961 cgaaaccatt ttgacggagg cgaaaatggc atacgggccg taaacgccgt tcgccattgc
    31021 gcccaggcag aatgcataag cggctttatt cactggccct ccaaggtcaa aggaacacat
    31081 aaaaccgaga atggcaccca gcaacagcgc gttacttcct gacagaccgt tcagccaggc
    31141 ggtaagcgag ttattgatcc aggcgacagg ttcccccacc acaaacagca tcagactgcc
    31201 cgctcccaat gtaccgagca ccgggtagag ataaaaagtc aggaagccat tgaatttact
    31261 gctaagacgc aagtgatttt tcatccagcg tatcaagtaa ccggctatca atccgccaac
    31321 gaccgcgccg agaaacccgg agccgatcat gttggcggca agtccagccg caaagcctgg
    31381 cgctaacgcc ggtttatctg ccagcgaata ggcggtatag gctgccagca ccggtaccat
    31441 caaaattccg agcagcccgc cgcccagttt gcggtacatc cacagccagg agttttcttc
    31501 attaaacaga tcttgtagcc cgaagatttg cgacagtaat accgcgaccg ccagcaccgt
    31561 cccccccgcg acaatcaacg gtacggcaaa agagatcccg ctcaacagtg cctgtttcag
    31621 ctccgttttg acgcttttca ccggttgcgt atcttgctgt aaggtacgcg tctcatcgcc
    31681 acgctcgagg gtaagcgatt gttggatcaa cgcttctgca tggcgaatcg gctcggcaac
    31741 aggcacagaa agcgcaggaa tgccgttaaa acgctcactc tccttgatgg cgacttcagc
    31801 cgcaaaaata caggcagttg cactattgag ttgatccgcc gttaaacgcc cttcaatacc
    31861 gttagcacct tgcttttcaa cgtagacatt cacgccgagt ttgcgtccgg ctttttccag
    31921 atactccgca gccatgtagg tgtgagcaat acccgccggg caggccgtta cgcaaacgat
    31981 agttggcgcg ttggaaaaag aggcagaagg ttgcgtgcct cccttgtcat ccagcgccga
    32041 gagcaattca tcaggcgtcg tcgccgactg tatacgcgcc cgaatctcat catccgccag
    32101 gcgcgtggtc agcgctgtca gcagttgcat gtgcgtagta cccgcttcat tgaggggaat
    32161 cgccagcaac accactaaat caaccgcttc cgggccatcc acgccttccc actgaagcgg
    32221 ctcgctgagt gtggcgaccg caaacgccgc ttcttttacc gcagcagttt tgccatgcgg
    32281 cacagccaac ccttcactta aggcggtcgg gccaaggctt tcacggcgat acacttcttt
    32341 cagaaattgc tcagtactgg aaattttccc cagagcagca agacgttgag tcaacgcgtg
    32401 aatggcctct tcacggctgg taaagcgcgc attcagacac aacgcatcgc ggtgggtcag
    32461 agtcgtcagg ttcatcatga tcctcacttg atcgttttta tagtcgcgcc agtgtgcccg
    32521 acaaaacaat accattgcgt aatcaatttc acttgattga aattatttgc attttatttg
    32581 tattgtttac aggatgagtt tatcgggcat aatggctaaa ttcgtattaa tgagatgcta
    32641 aataatggga cacaaaccct tataccgaca gattgctgat cgcattcgtg agcaaattgc
    32701 gcgtggcgag cttaaacctg gcgatgcgtt acctaccgaa tcggcattac aaacagagtt
    32761 tggcgtcagc cgggttacgg tgcgtcaggc tttgcgccag ttagtcgagc aacagatcct
    32821 cgaaagcatt cagggcagcg ggacttacgt caaagaagag cgggtcaatt acgatatttt
    32881 tcagttaacc agttttgatg aaaaactgtc ggaccgtcac gtcgatacac acagtgaagt
    32941 tctgatattt gaagtgattc cggctgacga ttttcttcag caacagctac aaatcaccgt
    33001 gcaggatcgt gtttggcatg tgaagcgcgt gcgctatcgc aagcaaaagc caatggcgct
    33061 ggaagaaacc tggatgccgc ttgcgttgtt cccggatctc acctggcagg tcatggaaaa
    33121 ttcgaaatat cactttattg aagaagtgaa gaagatggtt atcgatcgta gcgaacagga
    33181 aatcattcct ctgatgccaa ccgaagagat gagccgtctg cttaatatca gccagacaaa
    33241 gccgattctt gaaaaagtat cgcgtggata tttagttgat ggtcgggtgt ttgaatatag
    33301 ccgcaacgct tttaataccg atgactataa atttaccctg atagcgcagc gaaaatcatc
    33361 gcgataagca caaaaaggcc atcatacgat ggccaaccat gtcgaaaccg acagcccttc
    33421 gccattcctg tagagggaag cggcgagggc tatttcttat tacagatatt tatttcagaa
    33481 cagttttcag tgcttcaccg atatccgcca ggctgcgaac ggttttcacg cctgcggctt
    33541 ccagagcagc gaatttctca tccgcagtcc ctttcccacc ggcaatgatc gcacccgcgt
    33601 ggcccatacg tttgcctttc ggcgcagtca caccagcgat gtaacccaca actggcttgg
    33661 taacatgctc tttgatgtac gctgccgctt cttcttcagc gctaccgccg atctcaccga
    33721 tcatcacgat cgcttcggtc tgcggatctt tttcgaacat ttcgagaatg tcgataaagt
    33781 tagagccagg gatcgggtca ccgccgatac cgacacaggt cgactgaccg aaaccgtaat
    33841 ccgtggtctg tttaaccgct tcataggtca gtgtaccgga acgggaaacg atacccactt
    33901 tacccggttt gtgaatgtga cccggctgga taccaatttt gcattcaccc ggggtgataa
    33961 cgcctgggca gttcgggccg atcatacgaa cgcctgcttc gtccagtttc actttcacgg
    34021 tcagcatatc cagcgtcggg atgccttcag tgatggtgat aatcagtttg atgcctgcat
    34081 cgatagcttc cagaatggag tctttgcaga acggtgctgg tacgtaaata acagacgcgg
    34141 aagcgccagt agcagcaaca gcttcacgca cggtgttgaa caccggcagg ccgaggtggg
    34201 tagtgccgcc tttacctgga gttacgccac caaccatttt agtgccgtat gcaatggcct
    34261 gttctgagtg gaaagtcccc tggctaccgg taaagccctg gcagataacc ttggtgtttt
    34321 tatcaattaa aatggacatt atttcccctc cactgcggca acaacctgct gagctgcatc
    34381 cgtcagacct tttgctgcaa taatattcag gccgctgtca gccagtttct tcgcgccgag
    34441 ttcggcgttg ttaccttcca gacgtaccac gaccggtacg ttaacaccca cttccgctac
    34501 cgcgccgatg ataccgtcag cgatcaggtc gcaacgaacg ataccgccga agatgttaac
    34561 cagaacggct ttcactttgt cgtcagagag gatgattttg aacgcttcgg ttacacgttc
    34621 tttggttgcg ccgccgccaa cgtccaggaa gttagccggt tcgccgccgt gcagtttaac
    34681 tatgtccatc gtacccatcg ccagacctgc gccgttaacc atacaaccga tgttaccgtc
    34741 cagcgcaacg tagttcagtt cccactgtgc agcctgtgct tcacgcggat cttcctgcga
    34801 ctggtcacgc atttcgcgca gatcaggctg gcggaacagg gcgttgccat cagcgcccag
    34861 tttgccgtcg aggcaaatca gatcgccctg tttggtgatg accagcgggt tgatttcgat
    34921 cagcgccagg tcgcgctcca ggaaaatggt cgccaggccc atgaagattt tggtgaactg
    34981 ctgaaccagt ttaccttcca gacccagttt gaacgccagc tcgcgtccct gatacggcat
    35041 cgggccagtc agcggatcaa gcgcaacttt atggatcagg tgcggagttt cttccgccac
    35101 tttttcgatt tccacgccgc cttcggtgga ggccataaag accacgcgac gggaactacg
    35161 gtctacaacg gcaccgagat acagctcttt agcgatatcg gtcgccgctt caaccagaat
    35221 ctggttaacc ggttggccat tggcatctgt ttgatacgtt accagacgct tgcccagcca
    35281 gttttctgca aaagcacgga tgtcttcttt gctgtttaca actttcacac cgcccgcttt
    35341 accgcggcca ccagcgtgaa cctgacattt cactacccac ggaccggcac cgatttttga
    35401 agcggcttct tctgcttcgc gcggagtagt acaggcataa cccaccggtg ctggtaagcc
    35461 atagcgggca aacagttgtt ttgcctgata ttcatgtaag ttcatgtgtt ctgtccatcc
    35521 ttcagtaatc gttatctttt aaaccgtaga ccgacaccct atccacgtcg gtctacagtg
    35581 caggtgaagc ttaaactact acacgtccag cagcagacgc gtcggctctt ccagtaactc
    35641 tttgatcgtt accaggaagc ccacggattc gcgaccatcg atcagacggt gatcgtagga
    35701 cagcgccagg tacatcatcg gcaggatctc aacctgacca ttcaccgcca tcggacgatc
    35761 tttgatagcg tgcataccca gaattgcgct ctgcggcggg ttgatgatcg gcgtagacat
    35821 cagggaaccg aacacaccac cgttggtgat ggtgaagtta ccaccggtca gatcttcaac
    35881 cgtcagcttg ccatcacggc ctttgactgc cagctctttg attttcttct cgatgtctgc
    35941 catgccgagg gtatcgacat cacgcagaac cggcgtcacc aggccgcgcg gcgtagaaac
    36001 cgccatgctg acgtcgaaat agttgtggta aaccacgtca tcgccgtcga tagacgcgtt
    36061 cacttccggg taacgtttca gggcttcaac caccgctttc acgtagaagg acataaagcc
    36121 cagacggatg ccgtggcgtt tttcaaacgc ttcaccgtac tgcttacgca gatccataat
    36181 cggcttcatg ttgacttcgt tgaacgtggt cagcatggcg gtggagtttt tcgcttccag
    36241 cagacgctct gccacacgct tacgcaggcg agtcatcggg acacgttttt cactacgtgc
    36301 agccagagcc ggttgcgccg ccggagcagc cgctgccgga gcagactctt tcgccggggc
    36361 tttcgccaga tgtttttcca catcttcacg agtcagacga ccacccacac cggtgccttt
    36421 aatggcgctg gcgtcgagat tgtgttcagc cagcaggcga cggatcgccg ggcttaacgc
    36481 atcgttgttt tgctcttcca gagacgcctg ctggcgttgc gccggagtgg acgctttctc
    36541 ttcagatttg gcgctggttt ctttaccggc gctgttgcct tcacgcaggc gaccaaggat
    36601 ctgacgagac gttaccgttg taccttcatc ttccagaacc gcatccagaa tgccgtctgc
    36661 tgatgccggt acttccagta ccactttgtc agtttcgatt tctaccagca cttcatcacg
    36721 tacgactgcg tcgccgggtt ttttatgcca ggttgcgacg gtggcatcgg ctacggattc
    36781 aggcaggtca gggaccagaa tatctacgct actcattgtg tatcctttat ttattcgacg
    36841 ttcagcgcgt cattaaccag atcttgttgc tgtttctggt gaacggacat ataccctacc
    36901 gccggagagg cggaggccgg gcggcctgca taacgcagag aagccccaaa cggaatcact
    36961 tcacggaaat gatgctggct gcagtaccat gcgccctggt tgagcggctc ttcctggcac
    37021 cagacaaaat ccttgacgtg agcaaactgc tgcaacactt cctgcatcgc tttatgcggg
    37081 aacgggtaga gttgctcgat acgcacaatg gcgacatcgt gttgattgtt cttacgacgc
    37141 tgttccagca ggtcgtaata aaccttacca gaacacatca ctacgcgctt cacgcccttc
    37201 ggatcaagct cgtcgatttc accgatggct ggcaggaagg tgccgttcgc cagttcttcg
    37261 aggctggaaa ccgccagcgg atgacgcagc agggatttcg gcgacatcac gaccagcgga
    37321 cgacgcatcc cgcgcagcgc ctgacgacgc agcatgtggt aaacctgtgc cggggtagac
    37381 ggtacgcaaa cctgcatgtt ttgctcagca caaagttgca gataacgttc cagacgcgcg
    37441 gaggagtgct ccggcccctg cccttcgtaa ccgtgcggca gcaacatcac cagaccacac
    37501 atccggcccc atttctgctc gccagaggag atgaactggt cgataaccac ctgcgcaccg
    37561 ttggcgaagt caccgaactg cgcttcccag atggtcaggg tgcgtggttc tgcggtggca
    37621 taaccatatt caaacgccag cacggcttct tctgacagta cggagtccca gacacggaac
    37681 gcgccctgac cgttatggat gtgttgcagc ggcgtgtaag tggaaccgtt agactggttg
    37741 tggatcaccg cgtggcggtg gaagaaggta ccgcgaccgg agtcttcacc cgacaggcga
    37801 accggaatgc cttcatcaac cagcgtggcg taagcgaggt tttccgcacc gccccagtcg
    37861 aacagtttct caccggctgc catcgcctgg cgatcgccat aaatcttggc aacgcgagac
    37921 tgcatttcaa ctgcttctgg taccgtactg atacgtttag ccagttcctg caggcgcttc
    37981 atctcaactt tgttcgggta ctcttcgtcc cattcgtggt tgaggtacgg cgaccaggtg
    38041 aaagagtgca tgttcatcgg acgccactct gccactacgc aatcgccagc atccagcgca
    38101 tcgcggtaca ggttaaccat ctcggtggca tcttccagcg tcgccacttt ttcctgctcc
    38161 agcttgtcag cgtagatttt gcgcggtgtc ggatgttttt tgattttctg atacatcagt
    38221 ggctgggttg cgctcggctc gtcggcttcg ttgtggccgt gacggcggta gcataccagg
    38281 tcgatgaaga catcacgttt aaaggtgtta cggaaatcga gcgccagacg ggtcacaaag
    38341 gcaacggctt ccggatcatc cgcgttaacg tggaaaatcg gtgcctgaac catcttaccg
    38401 atatcagtac agtacggtgt agaacgggca tccagcgggt tagaggtggt gaaaccaacc
    38461 tggttgttga taacgatacg taccgtaccg ccaacttcat aaccacgcgc tttcgacatg
    38521 ttcagagttt cctgaaccac gccctgcccg gtcactgcgg cgtcaccgtg gatggtgatt
    38581 ggcagcactt tgttgctgtt cggctcatca agtctgtcca gacgggcacg aacagagccg
    38641 ataacgaccg ggcttacaat ctcaaggtga gacgggttaa acgccagcgc caggtgcacc
    38701 aggccgccat cggtctggaa gtcagacgag aagcccatgt ggtatttcac gtcgcctgtg
    38761 ccgaggtgtt ctttatgttt accggcgaac tcgtcgaaca agtcttgcgg ttttttaccc
    38821 agcacgttca ccagcacgtt cagacgacca cggtgcgcca tcccgagaac cacttcgcgg
    38881 gtgccgctgt tgccagcgtg gcggatcatc tctttaagca tcgggattaa cgcgtcaccg
    38941 ccttccagcg agaagcgttt tgcgccaggg aattttgcgc cgaggtaacg ctcaaggcct
    39001 tcagcggcgg tcagttcgct taagaagcgt tttttctctt cgctattgaa agtcgcgcga
    39061 ccagactcga tacgctgttg gatccagcgt ttttcttcgg tgctggtgat gtgcatatac
    39121 tcggcaccaa tcgggccgca gtaggtttgc ttgagggcgg aaataagctc gccgagtttc
    39181 atggtttctt tgccgctggc aaatgaaccg acgttgaagg tttcctggaa gtctgcttcg
    39241 gtcagatcgt ggaaagacgg atccagatcg gccactttat cttgctgcca cagtcccagc
    39301 ggatctagat tcgcatgctg gtgaccacgg aagcggtatg cgttaatgag ctgcaggact
    39361 ttaacctgct tcacattggt gtcagggtcg gagatcgttg aagagtaacg tgaagcgtct
    39421 ttcgccaggc ggcggaaata ttcacgcgtt tgagagtgga attgatccgg tttgactccc
    39481 gtaccaggta actgctggaa cgtcgaacgc cagttagcgt caaccgagtc aggatcggtt
    39541 aagaagtctt catagagctg ttctatccag ctctggtttg cgccagagag gtaagaagag
    39601 tccaaccagg ctttcaaagc gctgttctgc atcgtgatcc cttaagcatc ttttttatgc
    39661 ttacttcgcc gtggatacta ccacgcacag tgatgtgcgt gccggggttc acttgcgagc
    39721 tcttgtctac gtagtggctc gcgaaggaac ctttaaaaac tgtcttagtg tcaggggcag
    39781 tttttaaaga tttcctgcat ttctttgtta cgcctgatgc gctgcgctta tcaggcctgc
    39841 gggtaatgac ttgtaggccg gataaggcgt tcacgccgca tccggcactg gttgcctgat
    39901 gcaacgcttg cgcgtcttat caggcctaca gtttacgcat tacgttgcaa caacatcgac
    39961 ttgatatggc cgatggcgcg cgtcgggttc agccccttcg gacatacact gacgcagttc
    40021 atgatgctgt gacagcggaa tacgctgaat gcatcgctca aaccgtcgag gcggctgtca
    40081 gtctcggtat cacggctatc gatcaggaaa cgatatgccg ctaacaagcc tgccgggccg
    40141 ataaacttat cgggattcca ccagaaagac ggacaagagg ttgaacaaca tgcgcagaga
    40201 atacattcat acagcccgtc gagtttttcg cgctgctctg gcatctgtaa atgctcgcga
    40261 gctggcggat tttgtccatt attcaacagg taaggcttaa ttttctcata ttgcgcatag
    40321 aattgtccca tgtctaccac caaatcgcgg atcaccggta aacctggcag cgggcgaatc
    40381 acaatcttct tgcccggctg gttgagtgcc gaaatcgggg taatacaggc cagaccattc
    40441 ttgccgttca tgttcagacc gtcggaaccg cacacacctt cacggcagga gcggcggaac
    40501 gacaggctgg gatctttctc tttcagctgg ataagcgcat ccagcagcat catgtcgcga
    40561 ccttcttccg cttccagggt gtaatcctgc atacgcggag catcatcaac atccgggtta
    40621 tagcgataaa ttgaaaactc gagtctcatt ttcctgtctc cgcattagta agtacgaatc
    40681 ttcggcggga atgccgggcg cagtttcggt tccatgttga cgcttcggcg cgtcatggat
    40741 tccgactcag gcagatacag ggagtggcac agccagtttt catcatcacg atccgggaag
    40801 tcgaagcggc tatgtgcgcc acggctttcg gtacggaagt tggcagaaac agccgttgca
    40861 tacgccgttt ccatcaggtt atccagttcc aggcactcaa cgcgctgggt gttgaactcg
    40921 ctggaagtgt catccagacg ggcatttttc agacgctcgc ggatcacttt caactgctca
    40981 agccctttcg ccatcgcatc accttcacgg aagaccgaga agttatgctg catacattct
    41041 tgcagcgctt tacggatcgc caccggatct tcaccgttac gattattgtt ccaccggttc
    41101 aggcgatcca gagacgcttc aacatcagac tcgctggcat cgtgcagtgc gccctgctcg
    41161 gcgatagact cttgcagatg cagacctgcc gcgcgaccaa agaccaccag gtccagcagc
    41221 gagttgccgc ccagacggtt agcgccgtgt accgatacac aagcgatttc accaacggca
    41281 aacagtcccg gaacaaccac atcttcgcct ttctcattca cagtcagcgc ctgaccggta
    41341 actttggtcg gaataccgcc catcatgtag tgacaggttg ggataaccgg aatcggctct
    41401 ttcaccggat cgacgtgagc gaaggtacgg gaaagctcca ggatacccgg cagacgggat
    41461 tcgagaactt ctttacccag gtgatcgagt ttcagtttcg cgtgtggccc ccacggacca
    41521 tcacagccgc gaccttcacg gatttcaatc atgatggaac gcgccaccac atcacggccc
    41581 gccaggtctt tggcgttcgg cgcataacgc tccataaagc gttcgccatg tttgttcagc
    41641 agataaccgc cttcaccacg gcaaccttcg gtgaccagta cgcccgcacc ggcaatgccg
    41701 gtcgggtgga attgccacat ttccatatcc tgcaccggta cgcctgcgcg gatagccatg
    41761 ccgacaccgt cgccagtgtt aatgtgggcg ttagtggtgg actgataaat acgccctgct
    41821 ccgccagtcg ccagcacggt agcgcgggct ttgaaataaa ccacttcacc ggtttcgatg
    41881 cacagtgcgg tacaacccac caccgcgcca tcctggtttt tcaccagatc cagcgcatac
    41941 cactcggaga aaatggtggt gtggtttttc aggttctgct gataaagcgt gtgcaacagt
    42001 gcgtgaccgg tacggtcagc agccgccgca gtgcgtgccg cctgctcgcc gccgaagttt
    42061 ttcgactgac cgccaaacgg acgttgatag atacgaccat catcgagacg cgagaacggc
    42121 aggcccatgt gttcgagttc cagaatcgct tccggcccgg ttttacacat atattcaatc
    42181 gcgtcctggt caccgatata gtccgaccct tttacggtgt cgtacatatg ccattcccag
    42241 ttatcttcat gggtattacc cagcgcaacg gtaatgccgc cttgcgcaga aacggtatgg
    42301 gaacgggtcg ggaagacttt agagagcagg gcacaggtct ggccgctctg ggaaatttgc
    42361 agcgccgcgc gcatacctgc gccaccggca ccaatcacaa ctgcatcaaa ttctctgact
    42421 ggcaatttca tcacacaccc cacaccacaa cgaatccata aatcacgtaa accaccagtg
    42481 caacgacaat caccagttgc agcatcaggc gcaaggccag cggtttaacg tagtcggtca
    42541 acacctgcca catgccgatc caggcatgaa tcaagataga aaacagcgcc agcagggtga
    42601 acactttggt gaacgcagag gcgaagaaac cgatccagac ttcatatgtc agttcgccac
    42661 tggtggcgaa aaaaccgacc atataaatga tgtagagcgt caggacgata gcggtagcgc
    42721 gaacgaggat gaaatcatgt acgccattgc gtcctaatgc ggaggcgttg cttaccatac
    42781 gaggactcct gcgagaagtg aaagcacgac agtaataaca aaggagattt tggcggagcg
    42841 tttacccgct tcgaatgttt cttccagata gccaaaatcc atcatcatgt ggcgaatacc
    42901 tacgacgacg tgatacgcca gagcggtaag gatgccccac atgataaatt tgacgaagaa
    42961 gctgcccata atcgcggaag cttgctcgaa accttcaggg gaagagaggc tggtacccag
    43021 aagccactgc aggatgccca ctgcaacaaa ggtgatcaca ccggaaacgc gatggagaat
    43081 ggacgctatc gccgtgatgg ggaaccggat ggtctgtagg tccagattaa caggtctttg
    43141 ttttttcaca tttcttatca tgaataacgc ccacatgctg ttcttattat tccctgggga
    43201 ctacgggcac agaggttaac tttctgttac ctggagacgt cgggatttcc ttcctccggt
    43261 ctgcttgcgg gtcagacagc gtcctttcca taactgcgcg tcatgcaaaa cactgcttcc
    43321 agatgcgaaa acgacacgtt acaacgctgg gtggctcggg attgcagggt gttccggaga
    43381 cctggcggca gtataggctg ttcacaaaat cattacaatt aacctacata tagtttgtcg
    43441 ggttttatcc tgaacagtga tccaggtcac gataacaaca tttatttaat ttttaatcat
    43501 ctaatttgac aatcattcaa caaagttgtt acaaacatta ccaggaaaag catataatgc
    43561 gtaaaagtta tgaagtcggt atttcaccta agattaactt atgtaacagt gtggaagtat
    43621 tgaccaattc attcgggaca gttattagtg gtagacaagt ttaataattc ggattgctaa
    43681 gtacttgatt cgccatttat tcgtcatcaa tggattcttt acctgcaagc gcccagagct
    43741 ctgtacccag gttttcccct ctttcacaga gcggcgagcc aaataacaaa cgggtaaagc
    43801 caggttgatg tgcgaaggca aatttaagtt ccggcagtct tacgcaataa ggcgctaagg
    43861 agaccttaaa tggctgatac aaaagcaaaa ctcaacctca acggggacac agctgttgaa
    43921 ctggatgtgc tgaaaggcac gctgggtcaa gatgttattg atatccgtac tctcggttca
    43981 aaaggtgtgt tcacctttga cccaggcttc acttcaaccg catcctgcga atctaaaatt
    44041 acttttattg atggtgatga aggtattttg ctgcaccgcg gtttcccgat cgatcagctg
    44101 gcgaccgatt ctaactacct agaagtttgt tacatcctgc tgaatggtga aaaaccgact
    44161 caggaacagt atgacgaatt taaaactacg gtgacccgtc ataccatgat ccacgagcag
    44221 attacccgtc tgttccacgc tttccgtcgc gactcacatc caatggcagt catgtgtggt
    44281 attaccggcg cgctggcggc gttctatcac gactcgctgg atgttaacaa tcctcgtcat
    44341 cgtgaaattg ccgcgttccg cctgctgtcg aaaatgccga ccatggccgc gatgtgttac
    44401 aagtattcca ttggtcagcc atttgtttat ccgcgcaacg atctctccta tgccggtaac
    44461 ttcctgaata tgatgttctc cacgccgtgc gaaccgtatg aagttaatcc gattctggaa
    44521 cgtgctatgg accgtattct gatcctgcac gctgaccatg aacagaacgc ctctacctcc
    44581 accgtgcgta ccgctggctc ttcgggggcg aacccgtttg cctgtatcgc agcaggtatt
    44641 gcttcactgt ggggacctgc gcacggtggt gctaacgaag cggcgctgaa aatgctggaa
    44701 gaaattagct ccgttaaaca cattccggaa tttgttcgtc gtgcgaaaga taaaaatgat
    44761 tctttccgcc tgatgggctt cggtcaccgc gtgtacaaaa attacgaccc gcgcgccacc
    44821 gtaatgcgtg aaacctgcca tgaagttctg aaagagctgg gcaccaaaga tgacctgctg
    44881 gaagtggcta tggagctgga aaacatcgcg ctgaacgacc cgtactttat cgagaagaaa
    44941 ctgtacccga acgtcgattt ctactctggt atcatcctga aagcgatggg tattccgtct
    45001 tccatgttca ccgtcatttt cgcaatggca cgtaccgttg gctggatcgc ccactggagc
    45061 gaaatgcaca gttacggtat gaagattgcc cgtccgcgtc agctgtatac aggatatgaa
    45121 aaacgcgact ttaaaagcga tatcaagcgt taatggttga ttgctaagtt gtaaatattt
    45181 taacccgccg ttcaaatggc gggttaattt ttatatgcct aaacacaaaa aattgtaaaa
    45241 ataaaatcca ctatcggaca ttcatatatt ctaaaaaaaa atacatcggt ataaattata
    45301 aacaaataga tgatttttaa tcatctatct tcctctcagt tacatgaata aaccaccaaa
    45361 tatagcgcac ccctataatt cccaatactt tattcatcaa acatttaatt cataccatca
    45421 attaattact tttacattta ataaaattaa taatattttt aagaaaaact aaaaacattc
    45481 gacaatcaaa taagatattc ttgcaccgat atactttttt cttttctgtt aaaggactca
    45541 gttatgttta aaggacaaaa aacattggcc gcactggccg tatctctgct gttcacagca
    45601 cctgtttatg cggctgatga aggatccggt gaaattcact ttaaaggtga agttattgaa
    45661 gcaccgtgtg aaatacatca ggatgatatt gataaagagg ttgaactcgg tcaggtgacc
    45721 accagccaca ttaatcagtc acatcacagc gatgccgttg ctgtcgacct gctcttagtc
    45781 aactgtgatc tggaaaactc cagcaacggt tccggtggca agatttctaa agttgcagtc
    45841 acctttgata gctcagcgaa aaccaccggc gcagatccga ttctcaacaa caccagcacg
    45901 ggtgaagcca ccggcgtcgg cgtacgttta atgaataaag atcaaagcaa tatcgtttta
    45961 ggcaccgcta ctccagatat cgacctggct ccgacctcca gcgaacaaac gctgaatttc
    46021 tttgcctgga tggaacaaat tgatcaggct acacctgtaa cgccaggcgc agttacggcg
    46081 aatgcaacct acgtgctgga ttataaataa gtattacttt ggcggcgcta tcgccgcctt
    46141 tcataaaata tttatcaggt atggacaccg tgaatattta tcgactctct tttgtatcct
    46201 gcctggtcgt ggcgatgcct tgcgcattgg cggtcgaatt caatctgaat gttctcgata
    46261 aatcaatgcg cgaccgcatt gatatttcat tattaaagga aaaaggagtc attgctcccg
    46321 gtgagtattt tgttagcgtt gcggtaaata ataaccaaat cagtaacgag caaaagatta
    46381 actggcacaa aaatgacgat aaaaccattc cgtgcatcaa tgatttactg gtcgataaat
    46441 ttggcttaaa acctgaagtc cgtcagtcgt taccattgat aaatcagtgc gtcgatttta
    46501 gctcccgacc tgaaatgctc ttcaatttcg atcaagccaa tcagcaactg aatattagta
    46561 ttccgcaagc ctggctggcg tggcactcag aaaactggac cccaccctcc acatggaaag
    46621 aaggtgtcgc cggtgtcctg atggattaca acttgtttgc cagcagctac cgcccacagg
    46681 atggcagcag cagcactaat cttaacgcct atggaacagc cggaatcaac acgggggcat
    46741 ggcgcttacg cagtgattat cagttgaatc atactgatag cgatgataac catgaacagt
    46801 caggcgaaat atcacgtacc tatctttttc gcccgttacc gcaattaggt tcaaaattaa
    46861 ccctcggcga aacggatttt agtcccaata ttttcgacgg tttttcttat accggcgcgg
    46921 cactggcaag tgacgatcga atgttgccat gggaactacg cggctacgcc ccacaaatta
    46981 gcggtattgc acagaccaat gccacggtga cgatcagtca atcaggccgc gtcatttacc
    47041 agaaaaaagt cccaccaggc ccatttatca ttgacgacct taatcagtct gttcagggca
    47101 cactggatgt caaagtgacg gaagaagatg gtcgggtgaa caatttccag gtttcagcag
    47161 catcgacgcc cttcctgact cgtcagggac aggttcgcta taaactggcc gcgggtcagc
    47221 cacggccctc catgtcacat caaactgaaa atgaaacctt ttttagcaat gaagtttcct
    47281 gggggatgct gtcaaacacc tcgctgtaca gcggcctgct gctttctggt gatgactacc
    47341 attctgccgc aatgggtatt gggcaaaata tgctgtggct tggtgcgctg tcgtttgatg
    47401 tcacgtgggc cagtagccat tttgatactc agcaggacga gcggggctta agctaccgtt
    47461 ttaattacag caaacaagtg gatgccacta acagcacgat ttccctcgcc gcttatcgtt
    47521 tctccgatcg tcattttcac agctacgcca actatctgga tcacaaatac aacgacagcg
    47581 atgcgcagga cgaaaaacag acgatcagct tatctgtggg ccaaccgatt accccactaa
    47641 acctcaatct ttacgccaac ctgctacatc aaacctggtg gaatgcagac gcctccacga
    47701 ccgccaacat cacggcaggt tttaatgttg atattggtga ctggagagat atctctattt
    47761 ctacgtcatt caatacaacc cattacgaag ataaagatcg cgacaaccag atttacctgt
    47821 cgatttcgct ccccttcggt aacggtggtc gggttggcta tgacatgcaa aacagtagcc
    47881 acagcaccac acaccgcatg tcgtggaacg atacgctgga tgaacgtaat agctggggca
    47941 tgtctgccgg actgcaatcc gaccgtcctg acaatggagc ccaggtgagc ggtaactatc
    48001 agcacctgag ttcagcgggt gagtgggata tttctggtac ctatgccgcc aatgattaca
    48061 gttccgtcag cagcagctgg agcggttctt tcaccgcaac ccaatatggt gcagcgtttc
    48121 atcgccgcag ctccaccaat gaaccacgcc tgatggtcag caccgatggc gtggcagata
    48181 ttccggttca gggcaatctc gactacacca accattttgg cattgcggtg gtgccgttga
    48241 tttccagtta ccagccttcc accgtggcgg tgaacatgaa tgacttaccc gacggcgtaa
    48301 cagttgcaga aaacgttatc aaagaaacgt ggattgaagg cgcgataggt tacaaatcac
    48361 tggcttcccg ttccggtaaa gacgttaacg tcatcattcg caacgccagc ggtcagtttc
    48421 ctcccctcgg agcggatatc cgccaggatg acagcggcat tagcgtgggg atggttggcg
    48481 aggaaggaca tgcctggtta agcggagtcg ctgaaaatca aaagtttacc gtggtctggg
    48541 gtgatagcca gcattgctcg ctccatcttc ctgaacatat ggaagacacc gcaaatcgcc
    48601 tgattttacc ttgtcattaa ttaaggaaat gaaaatgaca tttatgaaag gactacccct
    48661 tttgttgttg gttgccagtc tgtgcagcca cgctgcacta caacccgatc gcactcgtat
    48721 tgtgtttaac gctaatgata aagctaccag tctgcgggtg gataatcgta gcgataaact
    48781 cccctacctc gcttattcct ggctcgaaaa tgaaaaaggg gagaaaagcg atgatcttct
    48841 ggtcgccctg ccgccgatac aacgtctgga accgaaagca acaacacagg tacggatagt
    48901 aaagcaagcc tcgaccacaa aattaccggg cgatcgcgaa acgctatttt tctacaatat
    48961 gcgtgaaatt cctcccgccc cggaaaaaaa cagcgaccat gccgttctgc aagtcgccat
    49021 tcagagtcgt atcaaggtgt tctggcgacc ggctgcatta cgcaagaaag caggagaaaa
    49081 ggtcgaactg cagttacagg tcagccagca gggcaaccaa ctgacactga aaaatcctac
    49141 cgcgtattat ctgaccattg cctacctggg acgaaatgaa aaaggcgttc tccccggctt
    49201 caaaaccgtg atggttgcgc cttttagtac ggtcaacaca aacaccggaa attatagcgg
    49261 cagccagttt tatctcggtt atatggatga ttacggcgcg ttacgcatga cgacgctcaa
    49321 ctgcagcgga caatgccatt tacaagccgt ggaggcgaag aaatgagtgc tggcaaggga
    49381 gtgttgctcg tcatctgtct gttatttctg tcgttgaagt ctgcgcttgc cctgaactgc
    49441 tactttggtt catccggtgg ctcggtagaa aaatcagaag cgattcaacc ttttgctgta
    49501 ccaggcaatg ccaaactcgg cgataagatc tgggaatctg acgatattaa aataagatct
    49561 gggaatctga cgatattaaa attcccgtct attgtgacaa caataccaac ggcaactttg
    49621 aaagcgaaca tgtctacgta tgggtaaacc cctacccggg ggtgcaggat cgttattatc
    49681 aactcggagt aacttacaat ggcgttgatt atgacgccaa ccagggaaaa agccgcatcg
    49741 acactaacca gtgtatcgac agtaagaaca ttgacattta tacccctgat gggatggcag
    49801 aacaaaattt gctcgggtga tcccgctaat attcacatgt cgcgaacctt tctcgctcgc
    49861 atgcggttat acgtcaaaat acgagaaatg ccgccgcatg attatcaaag tacgcttagc
    49921 gactatatcg tcgtgcaatt tgacggtgcc ggtagcgtta acgaagaccc tactgcccaa
    49981 aacctgaaat atcatattac tggtctggaa aacattcgcg tgctggattg cagcgtcaat
    50041 ttttccattt caccggaaac acagatgatt gattttggta aatttaattt gctggatatt
    50101 cgtcgccaca caatgtcgaa aacattcagt attaagacga caaaatcaca aaatgaccaa
    50161 tgcaccgacg gatttaaagt cagttcctcc ttttataccg aagaaacgtt ggttgaagaa
    50221 gataaagcat tgttgattgg taatggtctg aagctacgtt tgctggatga aaacgcctcg
    50281 ccttatacct tcaataaata tgctgaatac gccgatttca ccagcgacat gttggtctac
    50341 gaaaaaacct atacagctga actttcgtcc attgcaggta cccccatcga ggctgggccc
    50401 ttcgatacag tggtgctttt taaaattaac tataactgat ttgccatgat gccctttgca
    50461 gccggaaagg gcatctcttt cagaaaattg ctgattttcc ccttttcctt gattgctgtt
    50521 attttttcct agcccggctt tctttatgct gtatcgatac accaaagaga aaataatgag
    50581 ggagcgaagg atgccagttt tgcagtgggg aatgttatgt gtgctatcac ttctcctttc
    50641 tattggtttc ctcgcggtac atctcccggc ggcgctatta cttgggccga tgatcgccgg
    50701 gatcatcttc agtatgcgcg gaattactct gcaacttccc cgctccgctt ttctcgccgc
    50761 gcaggccatt cttggctgca tgattgcgca aaacctcact ggttcgattc tcaccacgct
    50821 ggccgccaac tggccgatcg tgcttgctat tttactggtg acgctgcttt ccagcgccat
    50881 cgtgggttgg ttattggtgc gctatagctc actgcctgga aataccggtg cctggggttc
    50941 ttctccaggc ggcgcagcgg caatggtcgc tatggcgcaa gattacggcg cagatattcg
    51001 cctggtggcg tttatgcaat atctgcgagt gctgttcgtc gcgggggccg ctgttctggt
    51061 cacccgcatg atgctgggtg ataacgctga agcggttaac cagcaaatcg tctggttccc
    51121 gccagtgagc attaatctcc tgcttacgat tttgctcgca gtcgttgccg gtacggcggg
    51181 atgcatgctg cgtcttcctt ccggcacgat gctcatcccg atgctggcgg gcgcagtgct
    51241 ccagtctggt cagctcatca ccatcgaact accggaatgg ctgctggcga tggcgtatat
    51301 ggcaattggc tggcggattg gtcttggttt cgataaacaa atcttactgc gggcactacg
    51361 cccgctaccg caaatcctgc tgtcgatttt tgctctgctg gctatttgtg caggtatggc
    51421 gtggggactg acccggttta tgcatattga ttttatgact gcctacctcg ccaccagtcc
    51481 cggcgggctt gataccgtgg cggtcatcgc cgcagggagc aatgccgata tggcgcttat
    51541 tatggcgatg caaaccctgc gcctgttcag tattttgctg acggggccag ccattgcgcg
    51601 gtttatttca acctatgcgc cgaagcggtc ggcctagtgc tggcagcctg gacaccagta
    51661 aaacgggcgc gaggacaacg tggttttctc aatgatgccg ccacagcgtt cgcacagttc
    51721 gccatcacga tgaaaaacct taaagcgaaa cagcgcccca tgatgcttat tctcatccac
    51781 ctgcccccgc gtagcgtaag aaaaccgagg aatctccagt aacgcgtgtg cgagtgcatc
    51841 cagttgcgcc gcattgagat ctttcgcttt atgatttcca gtcaacccaa cctgccagag
    51901 gatctccacc cgcaaataat tgccaagccc agccagaaac gcctgatcga gcagtaatcc
    51961 tgcaaactga cggttacgaa agcgcggcga caataaccgt tcctttacca cctccggcgt
    52021 cagattcgga tccagcacat cgggaccaac gcgttgtaaa aacggatgcg tggtcagttg
    52081 ttccggcctc aacatctcaa tatccgaggc gctataaagc agaatggttt tgtcagccgt
    52141 ttgcagtttt acccgcaata ctcgcgtggt ctgcggctct tcgccggtat caaccacgcg
    52201 ccagacgccg taaagctgat tatggctgta gagcgttaaa ccgttgggaa aatgagttaa
    52261 caacgcctta ccacgcgttt ccacatgggt aacgtgttga ccgataagtt gtgattgata
    52321 agtttttaac tgcgggaagg caaaccagac atcagttagt ggtttgcctt tgatcgccgc
    52381 ctccaggtta tccgctgcac ggcggatctc cgggccttca ggcatggtga tatccttttt
    52441 tcattcttta ttaagcgggg ttatgctgcg acaacaatcc cctttttggc aaatgtagct
    52501 cgtaatcggc gggcgaacgc cagagcgtgc tcgccgtcgc catgcaggca gaccgtttgc
    52561 gccgcgaccg ttgcccattc gccggtgata cttttgactc tgccgtgttg caccatctcc
    52621 agcgtttgcg ccaacgcctg ttcttcgttt tcaatcaacg cgcctggctg acttcgcggc
    52681 accagcgagc cgtcagcctg ataaccgcga tcggcaaaca cttcctcgcg cgttgtcagg
    52741 ccatattgct tgcctgcacg aatcagctcg cttcctgcca gcccaacgag aaccaatgcc
    52801 ggatcgcaag cgtataccgc tctggcgatg gcgtctgcca gttgtgcttc tttcgccgcc
    52861 tggttgtaca acatgccgtg cggtttgaca tgacgcatta cgccgccttg cgcacgggca
    52921 atcgttgcca gcgcgccaat ttgatacagc gtctgggcgt aaacggtttc cggcggcagc
    52981 tgcatggcgc tgcgaccaaa attttccctg tcgggaaaac tcgggtgagc gccaatcgcg
    53041 acaccatttt ttattgcttc acgcacgcaa gcctgcatgg tttgcgcatc gcctgcatga
    53101 aatccacaag caatattggc agaggaaacc agcgttaata gctctgcgtc gctggcgcag
    53161 ccttcgccca gatcggcgtt caggtcaatt ttcattgtgc agccgccacg ctaattgttc
    53221 aaaataacgt tgctgatctt gccgcgcttt cagtgcctct tccagtgaac actggacaaa
    53281 atgaatcggc tgaccgagcg gaatttgcgc cagatggtac atatcagcct caatgataca
    53341 ggcaatacgt ggataaccac cggtggtctg tgcgtcgttc atcaacacaa tcggctgccc
    53401 gttatgcggc acctgcacca cgcccggtaa caaaccgtga gataacagtt cgcgatcggt
    53461 gatgcgtttt aaaatttgcc cctgtaaacg atagcccatt cggttacttt gcgagctaag
    53521 ctgccagggc gaacgccaga atgcatcctg cgaggcgcga tcgaactcat gatattccgg
    53581 ccccggcaag gcgcgaatgc ggttgcccca cagcagctgt ttaacgccct gcgcttccat
    53641 aaaatcacgc ttcgatttgc caatcgggag tcagtcacca tccttcagta aacggccttc
    53701 cagcccgcca atccccactt tgagatcggt gctgcatgac cccattaccg gcggaacatc
    53761 aataccaccc gcgaccgcca gataactgcg catcccgtgc tgcgggcgtt taagcgttaa
    53821 acgctggcct gctttcatcg gcaatcgcca gccggtccag acggcgttat catccaaccg
    53881 cgcttcgcaa cctgcacccg tcagggcaaa ccacccatca gtttcgaact caactgttaa
    53941 ctgaccgagc gtgatctcca gcgcgggggc attggcgtca ttacccacca gcaggttagc
    54001 aatgcgcagc gcaggcatat ccagtgcgcc acagtggctg atacccgact ggcaaaaacc
    54061 gtgacgaccg ccatcctgca cagtggtgta catgcccgca cgaataatct tcagcatatt
    54121 ccctccttct gcggtacaaa tcgcacgctg tctcccggac gtaataagat gggttcgtca
    54181 cgcgccggat caaacaggct gagtgaggta tgaccaatca actgccagcc acccggcgtt
    54241 gccagcggat aaacaccagt ctgcggcccg ccgatcccga cagaacctgc cggaacgagc
    54301 aagcgcggtt cagcgcgccg tggcgtgtgt agttgttccg gcaaactccc gagatacggg
    54361 aagcccggtt gaaaacctaa aaaccagacc acgtattcca cggaggagtg caattcaaca
    54421 acctgttttt cgctcaaccc gcaatacgcc gcgaccaccg ccaaatccgg tccgcctgca
    54481 ccaccgtaaa ccaccggaat ttcaataaag cgagactcag gttccagggc ctcgctctcc
    54541 tcccaccagc gttgcaaacg ctctatggca tccagcgcca gcgactcagg attacgcaaa
    54601 atcaccgtga tattgttcat gccgggaatg gcttcaacca cattcggcat atccaccaga
    54661 cgctgcgcca gtcgccagat ccgtttctgg ctagccagcg tcaccggcgg ttccagttcc
    54721 agcactaccg ccgtttcacc tatcagataa caacgcgctc gttgcacttt cgatccctct
    54781 gattattcgt tatgcaggat taggaatatc aataaaggtc acatcaagat cggtattttc
    54841 attcagccac tcgctcaatg cgcgaatacc accgcgttca gtggcatggt gacctgcagc
    54901 ataaaaatgc aatccctgct cgcgggctga atgaatggtc tgttcagaaa cttcgccagt
    54961 aataaaagca tccacgccaa aacgcgcggc gctatcgata aaactttgcc cgccgcccgt
    55021 gcaccaggcg acgcgctgga ccacctcagg tccggtatcg ccacaccata acggcttacg
    55081 tcccagacgc gcttcaatcc aggaggctag ttccattccc ggcacaggca tggtcagctc
    55141 tccccacggc accaacggct caatttcgcc catgaccgtg atccccagta acgccgccag
    55201 ctgtgcgtta ttgcccagct caggatgtgc gtcaagcggc aaatgccagc catacaggtt
    55261 gatatcattc gccagcaacg tttttaaacg gttacgcttc attccgcgaa tgaccggaga
    55321 ctctcctttc cagaagtagc catgatgcac aatgaccgca tccgcgccca gacgcactgc
    55381 ctcatcgagc aaagcctggc tggcggtgac accggtaaca attttttgca ccgtctcttt
    55441 gccttccacc tgcaaaccat tcggcgcgta atcactaatc gccgcgctgt tcagtttttc
    55501 gttaatcagt tgttccagtt cggtgttttt catcttcgcg ccttccgtat tctgatagct
    55561 acaccgttaa catagcgtta catcgcctgt tcgtcgatag ccaatctttt gacatattaa
    55621 gaaacaaacc cgacgccaaa agacgcaaat tgtttatatc atcacgcttc agctaatgta
    55681 tatttatgca atcagacagc atattgcata aataatcaga tctgtatcaa agttttaact
    55741 ttaaggaaac acaagcacgc cgcgcaacta ttacgtgacg ttgccgtcaa ttgtggaatt
    55801 attatgaata aacacgcatc acaaccgcgc gctatttact atgtcgttgc gctgcaaatc
    55861 tgggaatatt ttagctttta cggcatgcgt gccctgctga ttctctatct caccaatcaa
    55921 ctaaaataca acgatactca cgcctacgag ttatttagcg cctactgttc gctggtgtat
    55981 gtcacgccaa tcctcggtgg ctttttggcg gataaagttc tcggcaatcg catggcggtg
    56041 atgctggggg cgttgttgat ggcgatcggt catgtggtgc tgggtgccag tgagatccat
    56101 ccgtcattcc tctatctgtc cctggcgatt atcgtctgcg gctatggcct gtttaaatct
    56161 aacgtaagct gtctgctcgg cgagctgtat gagccaaccg atccgcgtcg tgatggcggt
    56221 ttctcgctga tgtatgcggc gggtaacgtg gggtctatta tcgcacctat cgcctgtggt
    56281 tacgcccagg aagagtacag ctgggcgatg ggctttggcc tggcggcggt tggaatgatc
    56341 gcgggtctgg tcattttctt atgtggcaat cgtcatttca ctcatacccg cggcgttaac
    56401 aaaaaagtac tgcgtgcgac aaactttctc ctgccgaact ggggatggct gctggttctg
    56461 ctggtggcaa cgcctgcact gattaccgtg ctgttctgga aagagtggtc ggtatacgcc
    56521 ttaattgtcg cgactattat tggcctgggt gtactggcaa aaatttatcg caaagcagaa
    56581 aaccagaaac agcggaagga gctggggctg attgtgacgc tcaccttctt cagtatgttg
    56641 ttctgggcct tcgcacaaca gggcggtagc tcgattagcc tttatatcga ccgcttcgtt
    56701 aaccgcgata tgtttggtta taccgttccg accgcgatgt tccagtcgat taatgccttc
    56761 gcagttatgc tgtgcggtgt gttcctggcg tgggtggtta aagaaagcgt cgcaggtaat
    56821 cgtaccgtgc gcatctgggg aaaatttgct cttggccttg gcctgatgag cgccggattc
    56881 tgcattctga ccttaagcgc ccgctggtcc gcaatgtatg gtcactcttc tctgccactg
    56941 atggtgttag gcctcgcggt gatgggcttt gcggaactgt ttatcgaccc ggttgccatg
    57001 tcgcaaatta cgcgcattga aattccgggc gtgaccggcg tattaaccgg catctacatg
    57061 ctgctttctg gcgcgatcgc gaactatctg gcgggcgtga ttgccgatca gacatcgcag
    57121 gcttcgtttg atgcttccgg ggcgatcaac tactccatca atgcatatat tgaagtattt
    57181 gatcaaatta cctggggcgc actggcgtgt gtaggactgg tactgatgat ttggctgtat
    57241 caggcgctga aattcagaaa ccgcgcgctg gcgctggagt cataataggc cagcaccgat
    57301 gcctgatgcg acgcttgacg cgtcttatca ggcctacgta tttcctgcaa tttattgaat
    57361 ttgcacaaat ttgtaggctg gattaggcgt tcacgccgca tccggcatct gggctcgatt
    57421 gcctgacgcg tctgttattt ccccttccgc gccgcctcat acgctgccaa cgtttgtact
    57481 ctcgcctctt tgtgctcgac tatcggttgc ggataatcca gcttcacacc tgctttctgc
    57541 gcccacttcc acggctcatg caccgctttc cctggtacat cgcgcagctc cggtaaccat
    57601 cgacgaataa actcgccctc acggtcaaat ttctcgccct gagttatcgg attgaaaata
    57661 cgaaaatacg gcgcagcatc agtacccgta gaagccgccc actgccagcc accgttatta
    57721 gctgccaaat caccatcaat cagctgcgac atgaaatatc gctcgccttc gcgccagtcg
    57781 atcaacaaat ctttcaccag aaaactggct gtaatcatcc gtagccggtt atgcatccag
    57841 ccagtgctgt taagctgacg catagcggca tcaataatcg ggtatcccgt tttgcctttc
    57901 tgccaggcct gtaaatgtgc gggattgctt tgccactgca cacgatccgt ccaggcaata
    57961 aatggacgat gtttacacaa cgaggggtaa tacgtcatca gatgacggta gaactcgcgc
    58021 cagatcagct cactaagcca gacactaccg gccccaccgt ccagcgcctg cggctgttcc
    58081 gccaacaagc gatgcaaaca ctggcgaggc gataacccgc ccgttgccaa gctggcggac
    58141 aaacggctgg tgccttccac tgccggaaaa tctcgttgtt gctcatactc tccagcaccg
    58201 ttctcgcaaa actggcgtaa ttgcgcaatc gccgcttttt cttccaccgg gaaatgcgca
    58261 gtatcgaaag actgacgagg ataattcagc gtaatggatg gcgcgggctt tatcgatccg
    58321 ctactacgaa cttttggtgc agcgacgcac tccggcatcc cttcccgcag ccgtttcagc
    58381 caggcattct taaaaggcgt aaagactttg tacatctcgt gattgccggt catcaccgcc
    58441 cccggcggca ggatcacgct gtcatcaaat ccttcacaca ccacgttacg cagcgctctt
    58501 tcaacgtcca catcccgcgc tcgctcattc acctcatact gatagttata aaaaagatga
    58561 gtaacgctgt tttccgcgca cacctgttta actatttcga cactggcgac aaagtcatcc
    58621 acttcacgga acaataaagg aatacctttt tccgccagcg cgatttgtag cccattcagt
    58681 tgagcattga taagttcagc ctgacgcggc gacatgttat gcgtctccca cttgcgtggt
    58741 gtagcgatat acagcgccag cacgcgtgca gacgaattgc ggcaggcggc agccagtgcg
    58801 agattatcgt gcagacgtaa atcctggcga aaccaggcca gatgggtagt cataaaactc
    58861 ctgaatggcg caagttaatc acacgcggcc aaagttcgaa ataacgctgc cctgaaagcc
    58921 aggcgtcagg ataaagcgcc atatagtgtt tgatacgcat cagcggcgca agaagtggtt
    58981 gctcgccacg gcgataactg tcgataagcg cagccagctg ctggcgttct gtggaatcaa
    59041 tatgcgggcg aaaataaccc tgaacgtgca tcaaaacgtt ggtgtgatcg cgcgggttgg
    59101 cgggatgaga aagcaaaaca attacgcgtt ggcggtactg gttgtaaaaa tcatccagat
    59161 tttgccactg atgaatatca gccacaaacg ggccaagttt gcggtactcg ggctgagaat
    59221 gggccagaaa gacgagttta tagcggctgt gataatcgag aagcgccccg cgcgttaagc
    59281 catgttcttt cagtcgatgt aactcatgaa gggcgaagat acggattagg gtgctgtcat
    59341 caaatcgttg tagattcatt actgctcctg aacggtatac aggagtaagt gtaggcgttg
    59401 gtaaatgatg tttgtgaagt taatccgcta gcagggcaag attacgaaag cgcgctcccc
    59461 gcaagcaact aatcaaggta gttaatcatg gctgaacacg atattacgcc agaagtcact
    59521 atttccaaaa cacagcgccg ctataaagtc ggctatgtca gagcccgcca ccaggaccgc
    59581 aaaaccggta tagcccggta ttattcacaa catcccagtc tgcaccttaa gggcgactgg
    59641 ctgaaagagg ccggatttga taccggacgg ggtgtcacgg tgaagatttc ggaggggggc
    59701 ctgaccatca tcgctgacag tgacgaggtg caggaactgc gggatgggat gtttagtgtg
    59761 ctgaacgaaa gttaaatatt aaaaaagctg ggaagattta aaaccttcct agctttttat
    59821 tctgtcttat tataatggat actcatcatc ttccgatgaa tttataaatt gctccaagtt
    59881 tacatgaaga gtacttaacc tagtttttaa ctcattaagt gaagcgataa tttcatattt
    59941 tccaacataa tcatatgatg gatcgtattc attatctatg tcagaagcta aaaatgatga
    60001 tattcgtagt ttaaatttag cttcatcttt attacattca ttggcataag gtgaatgccc
    60061 tatcatagca tattcatcta atctaaaagg ataagatggt tttaattcat cgatcaatga
    60121 aatttcaatc catgttctat atccaggcat taatggacta atttctttga gcaaaacctt
    60181 tccacttaat aatgtattga gattgttcat tattatgtct cccacgggaa aaatatatgt
    60241 ctaacacttc caccatgttg atgaacttgt aaatgtggta aatcaccatc aggatttcct
    60301 ggtccatgac ctgacactct atatgaaggt tttgttacag aaggatcttt tatatcatcc
    60361 caatgatatg aacctgcttt tgttttattt tcagtaagat attccattaa gttcttagtg
    60421 ctggggcctg actcacctgt catattttta taattatgac ctaaatatct atccataaac
    60481 aattgttcag cgtcacttct agacctaaca gtaactttgg tttcactact atctaataaa
    60541 cgattcgctt tttggttact aacagcacat ggagttaatc caagtggatc agcatatttt
    60601 agaggattag gtgcataact gtagagattt aaaccgcctc tcaaccctat cgggtcagtt
    60661 accatgaacc gcccgacatc cggatcatag tacctgtgca gattgtagtg cagccccgtt
    60721 tccctgtcga ggtactgtcc ctggtagcgc aggttctgct ccaccccgcc gtggtccttc
    60781 tcctgtatcg tgttacccca gacctgatac cccgtctccc acacgatttt cccgtcgctg
    60841 tccgtcatct cttccggcgc gccgttcaca tccgtgtgaa aatacagtat ccggttcggg
    60901 ccctctttgc ccgccttatc cacccgcgcc agcggttcat aactcccctg gtccctgtag
    60961 atgtacaggc tgcctgttcc ctccggcctg ctctcctgcg ccagccgcat tccgtcccat
    61021 aaaaattttg tccggttgta cggcttaccg tcagggctga tgtgctgttt ttccgtccgc
    61081 ctccccagtg catcgtacac ataccggtat gtcccccgcg ctgtcgtcac ttccaccatc
    61141 cggtgttccg catcataacg gtaactctgc gttccgctcc ggccctcctt ttccaccgca
    61201 cggccaaatt cgtcataacg gtagtgcaca ccacggtaag acatcagccg gttgaacggc
    61261 accacgctgt tttctgccgt ggccctttcc cccgcgcgcc tccccagcag gtttcccgcc
    61321 gcgtccacct ccgtttccgg ctggtgccac accgggtgcc actgcgcctg ctccacccgc
    61381 cccagcgggt cgtgcgtctc cgtgatatgc ccctgcaggt catacacata gcgccatttg
    61441 ccgccctgag ggtcggtcat ccccagcaac agtcgttctt cgtcgctcca gcggaatgtc
    61501 gtcacctgtc cggcttcgtc ctggtacgcc gtcacctgat tctgcgcatc ccactggtga
    61561 taacgtgtgc gccccagtcc gtccgtcacg cacagcctgc gctgcgccag gtcccacgcc
    61621 agccggtact gttcaccgct gtccgtgtgg tgccccgtca cccgccatgc cgcgtcatcc
    61681 ggaccttcat accagcccca ctggtaataa tacccttcgc cgcccggctg gcggtgcagc
    61741 gtcatgcagt ggttttcgtc gtaagcaaac tcacgcagca ggtgccccgt gctgtcgtac
    61801 acgccgttca ggtggccggc ctcgtcatag ctgtaacgac gcagcagtaa aggtgcagtc
    61861 tcagggaaat gctgataaat ctgtgtcacc cgccggggat gcgccgccag ttcgtaatac
    61921 agacggatgc acggacgctg ctgttcgccg ctgatttcca caatgcggcc ccggtcgtcg
    61981 tagtacagat tcagacagtt gctgttacgg tcgcccagca ttttcagccg ctggcgctgc
    62041 gggtggtgcg ggtcagcctc aaacagaaaa cactgcccgt catcgctgct gataagccag
    62101 tgcccctgct caccacgcct taccgccagc ccttcaccgg ggctgtaaaa cccgcccccc
    62161 ggcagtacgg cttccatatc caagcgtcgc cccatgcccg acacaaaggt catgcagttt
    62221 tcgtctgcgc tacccggtgt gcacacgagg ctgacttcat acattacact ccagccctga
    62281 ccaaacattc cttccgtacg ttcgtcacga ctgctgtaca cgcgctgcca gtccagcgga
    62341 aagtggcccg gcagactgaa atcgaagtca tcgtcatcgg ccaggtattt ggccccggtg
    62401 gcggcattga ccggatgcgg aagggcactg gtggccttct gcgtcagcat gctcatcccg
    62461 acactggcag caaaacaggc gattttagaa agcattttgc ccgggcgaag aaacatcagc
    62521 gccacggttg ccaccagggt tatctgcgac ctgccgcttc tgatatccct gaccaccagt
    62581 aacggaccgc caatgcgcac atccggtgac acaaacgccc cgttccccgg ctcattcacc
    62641 accctggcct cgcaggtaca gcgtgccccg ctgcgtgtcg ccggctggct gttgatacgc
    62701 acatcccgcg cgccttccgc gatatattcc gctgcgggcg gctcgtcatc gcctttgaac
    62761 agattactga tgctgccaat aaccgcattc actccctgaa aggcggtttc cagcataccg
    62821 cccggattca gcaggttaag ggccgttccc agggggtcat ccagaaaggc ggcttttttt
    62881 tgtgcgagaa actgcgtcag tgaggaagga tgtttatggc agtcgatttt atcgtcaggg
    62941 gtcggggtgg atcccggggc cgcactggcc acagtgggct gccatagttc cgacacaaaa
    63001 tgtgtcacgc catccaccat ttcagaacgc ccttcttccg tactgagctg gtcgtgcaga
    63061 gcccgggaca tcagcacagg ccctgtcacc gggttaaccg ccagggctat ggcccccgcc
    63121 atttccagat attccccggc cacatcccgg aacgttctgg catcctcctg acgctgcgct
    63181 tcttcctgtt ctgccttcgc ctgcgcttcc tgcgcctcta tgtcctgccg cgatgccgca
    63241 gtcgccgctg cccgcgcggc aggtttgctg ttgatccatg tgtcaccaga gccggtgagg
    63301 atgtaacctt caatctgagg cgggaaaagc atatttgcga gggctgaaca ggcttcgctg
    63361 accttttctg tcacgccggt gtacaccatc gccacacccg ccacaaaacc ggtcagcacg
    63421 acggccgtgc accccatcgt gatccccact gacgtcgcca gtaacgaggt tgtgccgaca
    63481 atgagggcat caataacgac atttgcggcc acctccagca ccgcccccag cacatctgcc
    63541 ataaacgacg tatgcagcag ggcatcaccc tgtcttgccg cccagaatgc ctcttccatg
    63601 tcccgttccc tgtatgatta attcgtcctg acttcctgcc gcatcctgaa gcgcagattc
    63661 tttttaattt cagcccagcg cgtcatatct gccggggtca gcggtgtggt tctggcgtag
    63721 gtgaaggcca tcatgacccg ctcacccggc aatacaaatg ccacctgaca ctggtacacc
    63781 tgctcgttac cccggagaaa ctggctctgt atttccaaac cgcctcgttg agataatatt
    63841 ccgtcataat gtaaatagtc cgttttaata aaggaattat ttattgacag gttctgtttt
    63901 tttaatcatg aatgtaatac ccatcaccgc tttacagcac actttatctt tccagtcata
    63961 atcactaata atattaagca attagcaata tttacaacga ccagagcgga tacatagtta
    64021 tgtatttaac aatcccaata tattcactat gcaaataaaa ccactagtaa cagacacgct
    64081 accgcctcca tataaaaatg gacaccttat aaaattgtat cttgcataga aaaagctgaa
    64141 atatcggatg cgttttaatt atcaactata aatctataag ctttttatta acaaca
//
LOCUS       DAETQL010000012        60413 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.012, whole genome shotgun sequence.
ACCESSION   DAETQL010000012 DAETQL010000000
VERSION     DAETQL010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.012"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..316
                     /locus_tag="K0W65_000804"
     CDS             <1..316
                     /locus_tag="K0W65_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3094185.1"
                     /translation="GTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSN
                     SVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVT
                     YP"
     gene            429..1223
                     /gene="panB"
                     /locus_tag="K0W65_000805"
     CDS             429..1223
                     /gene="panB"
                     /locus_tag="K0W65_000805"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBI3094186.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1235..2086
                     /gene="panC"
                     /locus_tag="K0W65_000806"
     CDS             1235..2086
                     /gene="panC"
                     /locus_tag="K0W65_000806"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBI3094187.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="K0W65_000807"
     CDS             complement(2160..3062)
                     /gene="rpnC"
                     /locus_tag="K0W65_000807"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HBI3094188.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3336..3716
                     /gene="panD"
                     /locus_tag="K0W65_000808"
     CDS             3336..3716
                     /gene="panD"
                     /locus_tag="K0W65_000808"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBI3094189.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3720..4949)
                     /locus_tag="K0W65_000809"
     CDS             complement(3720..4949)
                     /locus_tag="K0W65_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBI3094190.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5013..5453)
                     /locus_tag="K0W65_000810"
     CDS             complement(5013..5453)
                     /locus_tag="K0W65_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBI3094191.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5558..6328)
                     /locus_tag="K0W65_000811"
     CDS             complement(5558..6328)
                     /locus_tag="K0W65_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3094192.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6325..7251)
                     /locus_tag="K0W65_000812"
     CDS             complement(6325..7251)
                     /locus_tag="K0W65_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3094193.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7360..8022
                     /gene="can"
                     /locus_tag="K0W65_000813"
     CDS             7360..8022
                     /gene="can"
                     /locus_tag="K0W65_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBI3094194.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="K0W65_000814"
     CDS             complement(8063..8599)
                     /gene="hpt"
                     /locus_tag="K0W65_000814"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBI3094195.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8805..11195
                     /gene="gcd"
                     /locus_tag="K0W65_000815"
     CDS             8805..11195
                     /gene="gcd"
                     /locus_tag="K0W65_000815"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HBI3094196.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="K0W65_000816"
     CDS             complement(11242..12792)
                     /gene="cueO"
                     /locus_tag="K0W65_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HBI3094197.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12958..13305
                     /locus_tag="K0W65_000817"
     CDS             12958..13305
                     /locus_tag="K0W65_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBI3094198.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13411..14277
                     /gene="speE"
                     /locus_tag="K0W65_000818"
     CDS             13411..14277
                     /gene="speE"
                     /locus_tag="K0W65_000818"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBI3094199.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14293..15087
                     /gene="speD"
                     /locus_tag="K0W65_000819"
     CDS             14293..15087
                     /gene="speD"
                     /locus_tag="K0W65_000819"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBI3094200.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15125..15487)
                     /locus_tag="K0W65_000820"
     CDS             complement(15125..15487)
                     /locus_tag="K0W65_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBI3094201.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="K0W65_000821"
     CDS             complement(15662..18259)
                     /gene="acnB"
                     /locus_tag="K0W65_000821"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBI3094202.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18614..20467
                     /locus_tag="K0W65_000822"
                     /pseudo
     CDS             18614..20467
                     /locus_tag="K0W65_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="K0W65_000823"
     CDS             complement(20538..21962)
                     /gene="lpdA"
                     /locus_tag="K0W65_000823"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBI3094203.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="K0W65_000824"
     CDS             complement(22287..24167)
                     /gene="aceF"
                     /locus_tag="K0W65_000824"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBI3094204.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="K0W65_000825"
     CDS             complement(24182..26845)
                     /gene="aceE"
                     /locus_tag="K0W65_000825"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBI3094205.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="K0W65_000826"
     CDS             complement(27006..27770)
                     /gene="pdhR"
                     /locus_tag="K0W65_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBI3094206.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28311..29684
                     /gene="aroP"
                     /locus_tag="K0W65_000827"
     CDS             28311..29684
                     /gene="aroP"
                     /locus_tag="K0W65_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBI3094207.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29727..30581)
                     /gene="ampE"
                     /locus_tag="K0W65_000828"
     CDS             complement(29727..30581)
                     /gene="ampE"
                     /locus_tag="K0W65_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBI3094208.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30578..31129)
                     /gene="ampD"
                     /locus_tag="K0W65_000829"
     CDS             complement(30578..31129)
                     /gene="ampD"
                     /locus_tag="K0W65_000829"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBI3094209.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31217..32110
                     /gene="nadC"
                     /locus_tag="K0W65_000830"
     CDS             31217..32110
                     /gene="nadC"
                     /locus_tag="K0W65_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBI3094210.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32313..32753
                     /gene="ppdD"
                     /locus_tag="K0W65_000831"
     CDS             32313..32753
                     /gene="ppdD"
                     /locus_tag="K0W65_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBI3094211.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32763..33910
                     /gene="tadA"
                     /locus_tag="K0W65_000832"
                     /pseudo
     CDS             32763..33910
                     /gene="tadA"
                     /locus_tag="K0W65_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33900..35102
                     /gene="hofC"
                     /locus_tag="K0W65_000833"
     CDS             33900..35102
                     /gene="hofC"
                     /locus_tag="K0W65_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HBI3094212.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="K0W65_000834"
     CDS             complement(35137..36180)
                     /gene="guaC"
                     /locus_tag="K0W65_000834"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBI3094213.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36342..36380)
                     /locus_tag="K0W65_000835"
     CDS             complement(36342..36380)
                     /locus_tag="K0W65_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094214.1"
                     /translation="MIIKTLKMSARK"
     gene            36405..37025
                     /gene="coaE"
                     /locus_tag="K0W65_000836"
     CDS             36405..37025
                     /gene="coaE"
                     /locus_tag="K0W65_000836"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBI3094215.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37025..37768
                     /gene="zapD"
                     /locus_tag="K0W65_000837"
     CDS             37025..37768
                     /gene="zapD"
                     /locus_tag="K0W65_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBI3094216.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37778..37975
                     /gene="yacG"
                     /locus_tag="K0W65_000838"
     CDS             37778..37975
                     /gene="yacG"
                     /locus_tag="K0W65_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBI3094217.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="K0W65_000839"
     CDS             complement(38075..38464)
                     /gene="mutT"
                     /locus_tag="K0W65_000839"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBI3094218.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38524..41229)
                     /gene="secA"
                     /locus_tag="K0W65_000840"
     CDS             complement(38524..41229)
                     /gene="secA"
                     /locus_tag="K0W65_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBI3094219.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41291..41803)
                     /gene="secM"
                     /locus_tag="K0W65_000841"
     CDS             complement(41291..41803)
                     /gene="secM"
                     /locus_tag="K0W65_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBI3094220.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="K0W65_000842"
     CDS             complement(42034..42951)
                     /gene="lpxC"
                     /locus_tag="K0W65_000842"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBI3094221.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="K0W65_000843"
     CDS             complement(43052..44203)
                     /gene="ftsZ"
                     /locus_tag="K0W65_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBI3094222.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="K0W65_000844"
     CDS             complement(44264..45526)
                     /gene="ftsA"
                     /locus_tag="K0W65_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBI3094223.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="K0W65_000845"
     CDS             complement(45523..46353)
                     /gene="ftsQ"
                     /locus_tag="K0W65_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBI3094224.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="K0W65_000846"
     CDS             complement(46355..47275)
                     /gene="ddlB"
                     /locus_tag="K0W65_000846"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBI3094225.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47268..48743)
                     /gene="murC"
                     /locus_tag="K0W65_000847"
     CDS             complement(47268..48743)
                     /gene="murC"
                     /locus_tag="K0W65_000847"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBI3094226.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48797..49864)
                     /gene="murG"
                     /locus_tag="K0W65_000848"
     CDS             complement(48797..49864)
                     /gene="murG"
                     /locus_tag="K0W65_000848"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBI3094227.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="K0W65_000849"
     CDS             complement(49861..51105)
                     /gene="ftsW"
                     /locus_tag="K0W65_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBI3094228.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51105..52421)
                     /gene="murD"
                     /locus_tag="K0W65_000850"
     CDS             complement(51105..52421)
                     /gene="murD"
                     /locus_tag="K0W65_000850"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBI3094229.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52424..53506)
                     /gene="mraY"
                     /locus_tag="K0W65_000851"
     CDS             complement(52424..53506)
                     /gene="mraY"
                     /locus_tag="K0W65_000851"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBI3094230.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53500..54858)
                     /gene="murF"
                     /locus_tag="K0W65_000852"
     CDS             complement(53500..54858)
                     /gene="murF"
                     /locus_tag="K0W65_000852"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBI3094231.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54855..56342)
                     /gene="murE"
                     /locus_tag="K0W65_000853"
     CDS             complement(54855..56342)
                     /gene="murE"
                     /locus_tag="K0W65_000853"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBI3094232.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56329..58095)
                     /gene="ftsI"
                     /locus_tag="K0W65_000854"
     CDS             complement(56329..58095)
                     /gene="ftsI"
                     /locus_tag="K0W65_000854"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBI3094233.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58111..58476)
                     /gene="ftsL"
                     /locus_tag="K0W65_000855"
     CDS             complement(58111..58476)
                     /gene="ftsL"
                     /locus_tag="K0W65_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBI3094234.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58473..59414)
                     /gene="rsmH"
                     /locus_tag="K0W65_000856"
     CDS             complement(58473..59414)
                     /gene="rsmH"
                     /locus_tag="K0W65_000856"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBI3094235.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59416..59874)
                     /gene="mraZ"
                     /locus_tag="K0W65_000857"
     CDS             complement(59416..59874)
                     /gene="mraZ"
                     /locus_tag="K0W65_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBI3094236.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14469 a        16732 c        14848 g        14364 t
ORIGIN      
        1 tggtactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgatg cagcaaaagg
       61 agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc
      121 taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg
      181 aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct
      241 acaacaggat ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca
      301 ggtaacctac ccttaataag tccaccgcac cgccatcatc tggcggtgcg gtaattgata
      361 aatcattgcc cccaccaatg tgactgataa aatgccaggc aacacgacat catttatcag
      421 gatacgttat gaaaccgacc accattgctt cacttcagaa atataagcag gataaaaagc
      481 gtttcgcgac catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaagggc
      541 ttaacgtcat gctggtaggc gactcgctgg gcatgaccgt tcagggccac gattccactc
      601 tgccagtaac cgttgctgat atcgcctacc acactgccgc cgtacgtcgc ggcgcaccaa
      661 actgcctgct attggctgac ctgccgttta tggcgtatgc cacgccggaa caagcctttg
      721 aaaacgccgc aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt
      781 ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag
      841 gtttaacccc acagtcagtg aatattttcg gtggctacaa agtacagggg cgcggcaatg
      901 aagcgagcga tcgactactc agcgatgcat tagccttaga agccgcgggg gcacagctgc
      961 tggtgctgga atgcgtgccg gttgagctgg caaaacgtat taccgaagca ctggcgatcc
     1021 cggttattgg tattggtgca ggcaacgtca ctgacgggca aatcctcgtg atgcacgacg
     1081 cctttggtat taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg
     1141 gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg
     1201 gcgaagaaca cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct
     1261 gctgcgtcag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac
     1321 tatgggtaat ctgcacaatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga
     1381 tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg aagatctggc
     1441 acgttatcca cggaccttgc aggaggactg cgagaagcta aacaaacgta aagtggattt
     1501 agttttcgcc ccttcggtaa aagagatcta cccgaacggt actgaaaccc acacttacgt
     1561 tgacgttcct ggcctttcga ccatgctgga aggtgccagc cgtccgggac attttcgcgg
     1621 cgtttcgact attgtcagca agctgttcaa cctggtccag ccggacatcg cctgcttcgg
     1681 tgaaaaagat tttcagcaac tggcgttgat ccgcaaaatg gttgccgata tgggcttcga
     1741 tattgagatt gtcggtgtgc caattatgcg cgccaaagac ggtctggcgc taagttcccg
     1801 taacggttat ctgacggcgg aacaacgcaa aattgcgcct ggtctgtaca aagttttaag
     1861 ttcgattgct gacaaattgc aggctgggga acgggatctc gatgaaatta ttaccattgc
     1921 ggggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga
     1981 cacattgctg gaagtttctg aaaccagcaa acgggcagta attctggtag ccgcctggct
     2041 tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact taactggcgc
     2101 tacggctgat ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact
     2161 taaataaggt taattacctt atcaatctca gcaagaggta aatttgccat ctctgcaacg
     2221 tcttcccgag acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt
     2281 cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc
     2341 atagacttcc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca
     2401 gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac
     2461 ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata
     2521 tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt
     2581 gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgcgc cagctccggc
     2641 gagtaaaaca tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa
     2701 aacagtatcg gcaccaccag cggcagctta tcgtggccag cctccagatg ccggtgcatg
     2761 gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt
     2821 tcaatcacaa catgcagata accgggattg ccctgcattt gcatggaata gagcacgtcc
     2881 gtgctgtgac ctttcaggca ctcttcaatg aaactccccg actctaaatg aagcgtgttg
     2941 agatcacaaa gttcgcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc
     3001 tccgcatgca ttaaaaattg tttaaatacc gcgtcatgcg gtgtggtact cggtgcatcc
     3061 atcttccctg tcttccgtgt ggccttgcgt gaaggcagta tccagtcaag gcgtgctatc
     3121 gtcagccacc tctttattgt ttaccgagca gcgttcataa aaatatcaat tcgttacagg
     3181 cgttacatgg cacgcttcgg cgcggtacgc aaactgctga cgcaaatgcc atcagatgag
     3241 tagacactca tcaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcaccgcc
     3301 tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca
     3361 aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg
     3421 accaggattt tcttgacgca gccggtattc tcgaaaacga agccatcgat atctggaatg
     3481 tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta
     3541 tttctgttaa cggtgcggcg gcccactgcg ccagcgtcgg cgatattgtc atcatcgcca
     3601 gcttcgttac catgccagat gaagaagctc gcacctggcg acccaatgtc gcctattttg
     3661 aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt
     3721 tatccctgcg gctggttact caccagccgc gacatcttct ccagcgaatc cgttcttaag
     3781 atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt
     3841 gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaagccg
     3901 ccaaacggat acgaaagata ccagacatgt ggattaaatt gcgccagagc gcggcgtgaa
     3961 cgtgcaaaat caaacagaat attgtgctca ctacggctca gtaatatggg tcggcgataa
     4021 ccatctaccc gatgcaaaaa atgggtatgt gactggaaat caaaaacatc gcgaatttcg
     4081 ttaagctcag aaacgctcat aaattgcagc gattttgggt tccacttctg cgggtgacgt
     4141 ttgatgcgtg aggtaacaat aaacaccgtc gccttcatgc catattgttt caacacagga
     4201 tacgcatagc ggctcaccga cttgaggcca tcatcaaagg taatcaccac cgctcgcgca
     4261 gggagattga tcgtattctt cacgtaacct tccagctgag ccatgctcag tgtcgcgtat
     4321 cccctgtcgc gcagccaggc catttggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa
     4381 gtatggcgaa aacgggtgtt ttcttcgtcg cgcagaatat ggtgataagt tagcaccggc
     4441 aggccattat cgggttgggc atccagcgcg ctgatatagg ccagtcgatc gccaatacgg
     4501 atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa
     4561 ttgtccgcca gtaccccaaa tggcgcactg cccacactcg gcgcgttgta gaccggcgta
     4621 tctttccagg taattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccgtct
     4681 tcaacttttt gtcgtccctg aactggctcg agatgccctt tatcgataaa ccctttacca
     4741 aagccaaaat taaatgcgta ataacttgcg gcagtgggct ccacggcaat gatttgtccg
     4801 gcccgaatat tccccacggt caccatcttg tcactaattt gcgcccagac cgcagcattt
     4861 tcgatggttt gcatataacg ggcaggtaac gcggcactga cacttgcggt aaacagcatc
     4921 agcaggagaa taacagcttg tttatacatg aaaaaataac cgagggtgaa acaacgcttt
     4981 tattgtagca aaagcgccat cagaatcgcg ggttaatact catacaaatt atgtttgagg
     5041 acgaaaggcg gatttttttg ttgttggtgc caaagactac tcacctccgg cccacccagt
     5101 tcgacaatac actcacgaaa ctctgaactg gtcatggttt cacggcaagc catcatctgt
     5161 tcaattaacg gtaacgtgac accagaaatc acttcgcaac gggagtgttt gtggcttaat
     5221 aatgaagcca cgcgatacgg tggcgctccg gctatatcgg ttaagaagat gacaccctca
     5281 ccagagtccg cttcatgcag agcatcgcac atcatgcggc tgagcatatt agagcttaat
     5341 ccgcgccaga aattcacggc ccggcactga agaagtgccc catgtttttt ctccagcgca
     5401 tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc
     5461 ctccttagcc gaaaatcagc ctaccctgac cattgttgat aaagggtgat agccgtcaaa
     5521 actcctctcc cccgaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac
     5581 gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa
     5641 aggtagtgac cagcggcaca tcatttatac cgaggaagcc gtagcggaaa ccactgatca
     5701 tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcagtg
     5761 agtaaaagac cccgcccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga
     5821 tgtcatcgaa cgttttggca aacacgccgt tcagcaaacc cgcaagggag aatagcaccg
     5881 ccgtgagcac cagggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa
     5941 acagtgaaat tgccgtcaca agaatgccaa caaacagacc acgcgccacg ccaccgccga
     6001 catatccggc aataatgacg tgagtcggaa ccggcgctac cagcagctct tcaatattac
     6061 gctggaactt ggcaccaaaa aatgatgacg cgacgttagc gtaggcgttg gtgatcaccg
     6121 acatcatgat cagccccggt acgatgaact gcatatagct gaagccatgc atatcgccaa
     6181 tacgcgaacc tatcaggtta ccgaagataa taaagtaaag ggtcatggtg atgactggcg
     6241 gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt
     6301 ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat taaccagtga
     6361 aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg
     6421 ctcacttaac tgcgtaaata cgctgttgat cccctgctca cgtagcactt caacttccag
     6481 cgtcgcggta tcgaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg
     6541 tgcgagatcg agaataaagg tttccgattt cagcttcgcc agcagcgcct tcatcgaggt
     6601 attttccacc agctcaccat gttgaataat gccgatattg cggcacagca tttctgcttc
     6661 ttccaggtag tgtgtggtaa gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa
     6721 gccccacatt gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag
     6781 tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga
     6841 taacatacgc gcacgttcgt tgcgttttcc ccataaatcg agctgcttaa gatatttttc
     6901 gctgcggatg tacgcctctt tgcgctccac accgtagtac cctgcctgat tcaccacaat
     6961 ttgctgcacg gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg
     7021 tttagcgttc acgacatctt tctcgagatc gtaaccaaat acgctgaccc gcccggaggt
     7081 tttatttacc agagagctga taataccgat agtggtcgat ttcccggccc cgttcggccc
     7141 gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct attccacgaa gcgcctgaac
     7201 gccgcctgga tagttttttt taagctgttg aagttccagt gcaatggtca taaattttta
     7261 cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt
     7321 agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc
     7381 atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag
     7441 aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt
     7501 cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct
     7561 aacctagtca ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta
     7621 ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgcc
     7681 gttgaaaacc cggaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg
     7741 ttcaaacata gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt
     7801 gaactgaacg tcatggaaca ggtgtataac ctgggccact ccaccattat gcaatcagcg
     7861 tggaaacgcg ggcagaaagt taccattcac ggctgggcct acggcattca cgacggcttg
     7921 ctgcgtgatc tggatgttac cgccaccaac cgcgaaaccc ttgagcaacg ttaccgtcac
     7981 gggatttcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc
     8041 aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgcca atatacggca
     8101 gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg
     8161 agaaaccgat aaattcaacc gggacgttca cttcacgacg ggacggttta tccagcagcg
     8221 tacaaatcgc caacgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg
     8281 tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat
     8341 ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg
     8401 cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca
     8461 taaatgagcc acgcagcaga cccaccagca ccatatcgct gccgctgtct ttgtaacgct
     8521 cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta
     8581 cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg
     8641 gcaggttttg gcaccgcaac aaagcgcaag acgcgcagta taccagtaaa actattcatt
     8701 cgcggaatct gttaataaag ctcgaattgt gatgacggtc acacatgtta aaacctataa
     8761 ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata
     8821 cagcctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt
     8881 atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg
     8941 ctggccttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgc gccgcgcttt
     9001 ggctatacgc agccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg
     9061 acttctgggc gctgactccg cgcagcgata ttctggtctt cttcggcatc tggctgatcc
     9121 tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg
     9181 tcgcactgct gattagcggt ggtatcctga cctgggccgg atttaacgat ccgcaggaga
     9241 tcaacggcac cttaagcgcc gatgccacac ctgccgaagc tatctccccc gttgccgatc
     9301 aggactggcc tgcgtatggc cgcaatcagg aaggtcaacg cttttcacca ctgaagcaaa
     9361 ttcacgccga taacgtccac aagctgaaag aagcctgggt gttccgtact ggcgatgtga
     9421 agcagccgaa cgatccgggt gaaatcacca atgaagtgac gccgattaaa gtgggcgata
     9481 ccctttacct gtgtaccgct caccagcgcc tgtttgcgct tgatgccgcc agcggcaaag
     9541 agaaatggca ttacgatcct gagctgaaaa ccaacgagtc tttccagcac gtaacctgcc
     9601 gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc gccggaagtg atggcggatt
     9661 gcccgcgtcg tatcattctt ccagtgaatg atggtcgcct gattgcgatt aacgctgaaa
     9721 acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc
     9781 cagacaccaa accgggtctg tatgagccga cttctccgcc aattatcacc gataaaacca
     9841 tcgtgatggc cggttcagtc accgataact tctcaacccg cgaaacgtct ggcgtgatcc
     9901 gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatccgggc gcgaaagatc
     9961 cgaacgcaat cccgtcagac gaacacacct ttacctttaa ctcaccaaac tcgtgggcac
    10021 cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg
    10081 atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc
    10141 tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg
    10201 acatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc
    10261 cgattattta cgctccggcg aaaaccggta acatttttgt gctcgatcgt cgtaatggcg
    10321 aactggtggt tccggccccg gaaaaaccgg ttccccaagg tgctgccaaa ggcgattacg
    10381 ttacccctac tcaaccattc tcggaactga gcttccgtcc gacgaaagat ttgagcggtg
    10441 cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga
    10501 tgcgctatga aggcattttc acgccgccat ctgaacaggg tacgctggtc ttcccgggta
    10561 acctggggat gttcgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg
    10621 ccaacccaat ggcactgccg tttgtttcga aactgctccc gcgtggtcct ggcaacccga
    10681 tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg
    10741 gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc atttggtctg ccatgtaaac
    10801 agccagcatg gggttatatc tcggcgctgg atctgaaaac taatgaagtg gtgtggaaga
    10861 aacgtattgg tacgccgcag gacagtatgc cgttcccgat gccggttccg gtgccgttca
    10921 atatgggtat gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg
    10981 ccgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc
    11041 agggtcgttt accggcgggt ggtcaggcaa caccgatgac ctatgaagtg aatggcaagc
    11101 agtacgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata
    11161 ttgtggctta tgccctgccg gatgatgtga aataaccata atgcccggag agatccgggc
    11221 atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg
    11281 ctccagcaga tggcagtgcg ccatataagc atgttctttc ggtgcgttgt gattaaactt
    11341 caccagcact tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg
    11401 agccgctggc ggtttgccat tttctgacaa gatacggaac tgcgtgccgt gaatatggaa
    11461 cggatgcagc atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc
    11521 cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg
    11581 gaaatcgaac ttcccgccgt ggttcatatg attcatattg ccgtgcccca tatggcccat
    11641 catctggctg tgatccattc cggccatcgc ctgatcgcca tatttctcca ttagcatctg
    11701 catccccatc atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc
    11761 ttccagcgaa ggtaacgcag gcaggctact taatgtgtca ggcaaagcac cggaggcact
    11821 aatagcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggtg caatcgccat
    11881 ccccatctgg ctgaccggca gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac
    11941 cagcacttca aaacgctcgc ccatcagcac cggcagctcg ctcaccttca ccggttcagg
    12001 tagcagacca ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg tggcgaaatt
    12061 gagcgagcgg gcattacagc cattgagcaa acgcaggcgc agccaaccac gcggggcagc
    12121 gtgttgtggg tagattgcac cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc
    12181 ggtcatcaca tccagttgat aatcaatctg cccgtcggcg ctaaatttct tatcctgaac
    12241 gatcaccgga acatcatcga taccccactg ttttggcagc attaatttca ggatctcgtc
    12301 atcttcaatc accaccagac cagccagccc catcgccacc tgtcgcccgg tcttgccatg
    12361 ttgatgtgga tggaaccagc aggtagcggc aggttgatca acgttcaacg tcaccgagcg
    12421 cttgccacct ggcggaataa ttccctgtgg gccgccgtcg acgtcacccg gtacttccag
    12481 cccgtgccag tgcaacgtcg tctcttccgc cagttggttg tagatatcaa ccgttaccgc
    12541 tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt
    12601 tgcagttttc tcgccaaagg tggactgacc tgcgccaata gttaactgaa tgcgattacg
    12661 ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc
    12721 acggctccac agtggcaagg ctgaagccac acccagtgcg acggaatatt tcaagaaatc
    12781 acgacgttgc atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc
    12841 atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt
    12901 cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg
    12961 aagacgtttt tcagaacagt gttattcggc agcctgatcg ccgtttgcgc aaacagttac
    13021 gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg
    13081 aagaacgatt gtggttacca gaacttatct aacgggcaaa ttcgtcgcgc actggtcttt
    13141 ttcgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagctctc
    13201 ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaaatgcaaa
    13261 gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt
    13321 tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt
    13381 tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg
    13441 ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc
    13501 gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat
    13561 ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt
    13621 ccgctactgg cccacggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc
    13681 atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat
    13741 gcgggtgtcg tgtcgttctg ccgtcagtat ctgcccaacc ataacgccgg tagctacgac
    13801 gatccgcgct ttcaactagt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag
    13861 acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga aagcctgttc
    13921 acctcagcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca
    13981 caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc
    14041 cattacttca gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc
    14101 atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt
    14161 caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg
    14221 gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga
    14281 gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg
    14341 agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt
    14401 gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt
    14461 tccattatcg gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc
    14521 agcgtcacta ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa
    14581 caccccggcc cactgccaga aacggtggtt gctcatctcg ataaaagtca tatttgcgta
    14641 catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa
    14701 gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt
    14761 gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttactcgcga cattaacggt
    14821 atgaagcact ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg
    14881 aaggcgctgt atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag
    14941 atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta
    15001 accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat
    15061 tacgggcgca atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat
    15121 ttatttactt ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa
    15181 cgccgcatag cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc
    15241 cagcgaattc cagttgattg gcgcgaacca tcacctcttc agcgtccatc cacagggtgt
    15301 attcatgccc tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt
    15361 ccacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca
    15421 cttcatgccc catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt
    15481 aatccatagt gtgttcctct gctcccggca atgcgccgaa atcgcatcgc gccattgatg
    15541 taattatcgc agccttaccg gagaaaaaca gcggaacggc gatagtttag aaataaaaaa
    15601 tgcccgggaa tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt
    15661 attacaccgc agtctggaaa atcaccccat cggctttttc ggtgtactga gaaagctggt
    15721 tgaagttcag ataacgataa gtatcaacgg cagttttatc tacttgcgca acgtaggtct
    15781 ggtactcttc cggcgtcggc agtttgccaa tcagcgccgc aacagccgcc agttctgcag
    15841 aagccaggaa gacattcgcg ccagtaccca aacggttcgg gaagttacgg gtagaggtgg
    15901 aaaccaccgt cgcaccgtct gctacacgcg cctggttacc catacacagg gaacagcccg
    15961 ggatctcgat acgagccccg ctcttaccga agacgctgta gtagccttct tccgtcagtt
    16021 gcgcggcgtc catacgggtt ggcggtgcca cccacaggcg ggtcggcaac tgacctttgt
    16081 gcgcatccag cagtttacca gcggcacgga agtgaccgat gttagtcatg caagaaccga
    16141 taaatacttc gtcgatcttc tcgccctgta ccgcagacag cggacgtgcg tcatccggat
    16201 cgttcggagc acacaggatt ggctctttaa tctccgccag atcgatgtcg atcactgccg
    16261 cgtattccgc atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc
    16321 cctgaatacg gcgttccagg gtacgacgat cgccgtaacc ttccgcgatc atccacttca
    16381 gcaggacgat gttggagctc aggtattcga tgatcggttc tttgttcagc ttgatagtac
    16441 aaccagcggc agaacgctcg gcggacgcat cggttagctc aaaggcctgc tcaactttca
    16501 gatccggcag accttcaatt tccagaatgc gaccagagaa gatgtttttc ttgcctttct
    16561 tctcaacggt cagcagacct tgtttgatcg catacagcgg aatggcgtgt accagatcgc
    16621 gcagggtgat gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat
    16681 ccagcggcat tacgccagtt gcggcagcaa acgccaccag accagaaccc gccgggaaag
    16741 agataccgat cgggaaacgg gtatgggagt caccaccggt accgacggta tccggcagca
    16801 gcatacggtt cagccaggag tgaatgacgc cgtcacccgg acgcagcgac acaccgccac
    16861 ggttcataat gaagtccggc agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg
    16921 ccgcggtgtg gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt
    16981 ctttcagttc atcacgggtc atcgggccgg tggtgtcctg agagcctaca gaagtcattt
    17041 tcggttcgca gtacgcgccc ggacgaatgc ctttcacgcc acaggcacgg cctaccattt
    17101 tctgcgccag cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat
    17161 cactgtgcgg cagaccaagt gcttcacgcg ctttggtggt caggccacgc ccgataatca
    17221 gcggaatacg gccgccagca cgcacttcat caatcagcac gtcggttttc agttcgaagg
    17281 tcgccagcag ttcgcctgtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa
    17341 tcacgtcgcc catgttcagg ttagagacgt tgacttcgat tggcagtgca cccgcgtctt
    17401 ccatcgtgtt aaagaagatg ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt
    17461 tgttcggcac atgtggaata tcatcgccca taaaccacag aacggagttc gtggcggatt
    17521 tacgcgatga acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt
    17581 gcagagcttc aatctgtttg atcgggccaa caacacctgg ctggtctggc tcaatacctt
    17641 cacgggcgtt tttcagcatc gccagcgcgt gcagtgggat atccgggcgt gaccacgcat
    17701 ccggtgccgg agaaaggtca tcggtgttgg tttcgccagt gactttaaag acggtaacgg
    17761 tcagtttttc agccagcgcc gggcgattca ggaaccattc ggcatccgcc caggactgca
    17821 ttacctgctt cgcatattcg ttgcctgctt tcgctttctc ttctacgtca tagaagttat
    17881 cgaacatcag cagcgtgtga gaaagtgctt tggcagcaat aggtgccagt ttggcatcat
    17941 ccagcgcgtc gatcagcgga tgaatgttgt aaccaccctg catggtgccc agcagttcga
    18001 tagctttttc cggagtcagc agaggggatt tagcttcgcc tttagcgata gcagccagga
    18061 agcctgcttt tacataggcg gcttcatcga cgcctggggg aacacggttg gttaacagat
    18121 ctaacaggaa ttcttcttcg cccgcgggcg ggtttttcag cagctctaca agcgcggcca
    18181 tttggtttgc atccaggggt ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct
    18241 tacggtattc ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga
    18301 ttctcttcac gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg
    18361 cagaatagca ggattttgac gacgtgttaa tctgtttaca aaaaagcaac ataaaaaagt
    18421 ggctgaatcg ttaaggatgg tctagagatt gtttccctcc gtaaaaactt cgttaattcg
    18481 catggtgata atcacctttc tcaacgaatc ccaagattag tcaaaattta aactaccgcc
    18541 tctttatact cggattcaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct
    18601 tgagatccga attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg
    18661 cagtgccggg ctttgtgctg cctctgccgg gctatatata aaaagccgca cagtggaagc
    18721 gcctgtagaa ccgcaatcga cacaactggc tgtgtctgac gctgccgctg ttacgcttcc
    18781 tgcaacggtt tccgcacctc ccgtaatacc cgcagtcgtc aaatccgcat tcagcactgc
    18841 acaaatagat caatgggtcg cacccgtcgc gctgtatccc gacgccctac tttcgcaggt
    18901 gctgatggca tcaacctatc cgacaaacgt tgctcaagca gtgcaatggt cgcacgataa
    18961 tccacttaaa caaggcgatg ctgctattca ggcggtatct gaccagccgt gggacgccag
    19021 cgttaaatca ctggtggcct ttccacaatt gatggcattg atgggcgaaa acccgcaatg
    19081 ggtgcaaaac ctgggcgatg cttttctggc gcagccgcag gacgtgatgg actcggtaca
    19141 gcgattgcgg caactggcgc aacaaaccgg ctcgctgaag tcatcaaccg aacagaaagt
    19201 tattaccaca acgaagaaag ctgtaccggt aaaacagaca gtcacggcac ccgtcatacc
    19261 atccaatacc gttttaactg ccaaccccgt cattacagag ccggcaacaa ccgtcatttc
    19321 cattgagccc gccaatcctg atgtggtcta tattcccaac tacaacccaa ccgtggttta
    19381 cgggaactgg gccaatactg cgtatccgcc ggtttatctg ccaccaccag ccggagaacc
    19441 gtttgttgac agctttgtgc gcggattcgg ctatagcatg ggcgttgcta ccacgtacgc
    19501 actattcagc agcatcgact gagatgacga cgatcatgac catcatcatc atgacgatga
    19561 tgattatcat caccacgatg gcggtcatcg tgacagtaat ggctggcaac acaacggcga
    19621 caacatcaat atcgacgtca acaatttcaa ccgtatcacc ggtgagcatc ttactgataa
    19681 gaatatggca tggcggcaca atccaaacta ccgtaatggt gtgccctatc atgatcagga
    19741 tatggcaaag cggtttcatc aaaccgatgt caacggcgga atgagtgcca cgcagctacc
    19801 tgctccaaca cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca
    19861 cgccgccccc gtcattacac gagataccca acgtcaggca gcggcacagc ggtttaatga
    19921 agctgaacac tatgggagct atgacgactt ccgcgacttc agccgtcgcc aaccactgac
    19981 ccagcagcaa aaggacgccg ctcgtcagcg ttatcagtcg gcctctcctg agcagcgcca
    20041 ggcagtccgc gagaaaatgc agactaaccc gcagatccag cagcgaaggg aggcggcgcg
    20101 ccagcgtatt cagtccgcct cgcctgagca gcgccaggcg gttcgcgaga aaatgcagac
    20161 taacccgcag aaccagcagc gaagagaggc agcgcgtgag cgtattcagt ctgcaactcc
    20221 cgaacagcgc caggtgttta aggaaaaagt acagcagcgc ccactgaacc aacagcaacg
    20281 tgataacgcc cgccagcgta tacaatcggc atcacctgaa caacgtcagg tttttcggga
    20341 gaaagttcag gagagccgcc cacaacgtct aaacgacagt aaccatactg tcaggctgaa
    20401 taacgagcaa cggtcagcag tacgcgaacg tctctctgaa cgtggagcaa gaagactgga
    20461 aaggtaaatt gcagacgtaa aaaaagcggc gtggttagcc gcttttttaa ttgccggatg
    20521 ttccggcaaa cgaaaaatta cttcttcttc gctttcgggt tcggcaggtc ggtaatgcta
    20581 ccttcgaaca cttctgccgc caggcccaca gactcgtgca gagtcgggtg cgcgtggatg
    20641 gtcagtgcga tgtcttcagc atcacaaccc atttcgattg ccaggccgat ttcacccagc
    20701 agctcgccgc cgttggtacc aacaatcgca ccaccgatca cacggtgaga ttctttgtcg
    20761 aaaatcagct tggtcatacc gtctgcgcag tcagaagcga tagcacgacc agaagcagcc
    20821 cacgggaagg tggcggtttc atagctgatg cctttctctt tcgcttcttt ctcagtcaga
    20881 cctacccatg caacttctgg ttcggtatag gcgatggacg ggataacttt cggatcgaag
    20941 tagtgtttct taccggcgat aacttcagcg gcaacgtgac cttcgtgaac acctttgtgt
    21001 gccagcatcg gctgaccgac gatatcgccg atagcaaaga tgtgcggtac gttggtacgc
    21061 agctgtttgt caacgcggat gaaaccacgg tcgtcaactt ccacgccagc tttgcctgcg
    21121 tcgaggtttt taccgttcgg cacacgacca atcgctacca gcacggcgtc gtaacgctgc
    21181 ggttcagctg gtgctttttt gccttccatc gtcacataaa taccgtcttc tttcgcttca
    21241 acggcggtaa ctttggtttc cagcatcagg ttgaatttct tgctgatacg cttggtgaag
    21301 actttaacga tgtctttgtc agctgccggg ataacctggt cgaacatttc aaccacgtca
    21361 atctgtgaac ccagcgcgtg gtaaacggtg cccatttcca gaccgatgat accgccaccc
    21421 attaccagca ggcgttctgg tacttctttc agttccagcg cgtcagtgga gtcccagata
    21481 cgcggatctt catgcggaat aaacggcagt tggatcgggc gagaacccgc tgcaatgatc
    21541 gcattgtcga agttgatcac ggttttgccg ttctcacctt caacttccag ggtgttagcc
    21601 ccggtgaatt tacccagacc gttgaccact ttgactttgc ggcctttcgc cataccagcc
    21661 agaccaccgg tcagctgatt gatcactttc tctttccagg tacgaatctt gtcaatatcg
    21721 gttttcggtt cgccgaagac gataccgtgt tcagccagcg ctttggcttc ttcgataact
    21781 tttgctacgt gcagcagtgc tttagaaggg atacagccga cgttcaggca aacaccgcca
    21841 agggtgttgt aacgttctac gattacggtt tccagaccta aatcagcgca acggaaggca
    21901 gcggagtaac ctgcggggcc tgccccaagt accacgacct gagttttgat ttcagtactc
    21961 atcatgacct ctatatattt atctccggcg gtcatacccg tcgtctttca ggccgcagcg
    22021 gcgttagctt cacgccctca ccccagtcac ttacttatgt aagctcctgg ggattctggc
    22081 gcttgctacc ttgctgcacc cagaaatcca tagggtatgt gaagtaatac cctaaccacc
    22141 accgggtcgt tctatccgtc ggtattttac aaaattgtta acaattttta aacaacaaac
    22201 ggcaaccgat ttgtctattc gctaataacc tcaatacatt catgagatta ccagaaaaaa
    22261 gccggccgtt gggccggctc ttttacttac atcaccagac ggcgaatgtc agacagcgtg
    22321 ttgttaatga tggtaatgaa acgggcacca tcagcaccgt cgatcacgcg gtggtcgaag
    22381 gagagagaaa tcggcagcat cagacgcggc acgaactctt taccattcca caccggctcc
    22441 atcgcggact tggaaacgcc gaggatagcc acttccggcg cgttcacaat cggcgcgaag
    22501 tgggtagtac ccaggccgcc gatgctggag atggtgaagc aaccgccctg catttcgccc
    22561 gcagtcagct taccgtcacg cgctttctta gaaatagtca tcagctcgcg agacagctcg
    22621 atgatgcctt tcttgttgac gtctttgaat accggaacaa ccagaccgtt cggggtatcc
    22681 accgccacac cgatgttgat gtatttcttc agggtcagac gctgaccgtc ttccgacagc
    22741 gaactattga agcgaggcat ctgctcaaga gctgcagcaa cggctttcat gatgaagaca
    22801 accggggtga tcttcacatc cagcttacgt ttcgccgctt cttcgttctg ctgtttacgg
    22861 aacgcttcca gctcggtgat atcggttttg tcgaagtggg taacatgcgg gatcatcacc
    22921 cagttacggc tcaggttagc accagaaatt ttctggatac ggcccagttc cacttcttcg
    22981 atttcgccaa acttgctgaa gtccaccttc ggccacggca gcatgccagg gataccaccg
    23041 ccagtcgccg ccggagctgc ttctgcacgt ttgatagctt ctttcacgta agcctgaacg
    23101 tcttcgcgca ggatacgacc tttacggcca gtgcccttca ctttcgccag gttaacgcca
    23161 aactcgcgtg ccagacggcg gatcagcggg gtcgcgtgaa cgtaagcgtc gttttcagca
    23221 aattcagatt tgccttccgc tttcgcagcc ggagcttcag cttttgctgc cggagccggc
    23281 gctgccgctt cctgtttcgc aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc
    23341 ataatcagcg aaccagtttt cactttatcg ccaacgttga ctttcagttc cttcacgacg
    23401 cctgcaaacg gagccggaac ttccatagaa gctttatcgc cttctacggt aatcagtgac
    23461 tgttcagcgg caactttgtc gcccactttc accatcactt cggtcacttc aacttcgtca
    23521 ccgccgatat ccggaacgtt aacttctttc acgccagctg ctggtgcagg ggtcgctgcc
    23581 ggagtcgctt cctgtttagc cgccggagct gccgcgcctg cttcacccgc gacttcgaag
    23641 accataatca gcgagccggt agacactttg tcacccacgt tcactttgat ctctttcacg
    23701 gtacctgcaa acggagccgg aacttccata gaagccttgt cgccttctac ggtgatcagc
    23761 gactgttcag cttcaacttt atcgccaact ttcaccagga tttcggtcac ttcaacttcg
    23821 tcgctgccga tatccggaac gttaacgtct tttgccgccg cagccgcggg tgctgctgcc
    23881 ggagctgctt ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg
    23941 gaatcgaaaa tcataatcag tgcgccggtc tgggttttat cgccaacaga gactttgatc
    24001 tctttaacga tacccgcctg cggagatgga acttccatag aggctttgtc gccttctacg
    24061 gtgatcagcg actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt
    24121 tcaacttcat cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct
    24181 cttacgccag acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag
    24241 caaccacttt cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gcaaccacga
    24301 cataagaagc atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga
    24361 agccatcagt acccagtacg cgatagtcgt cagccggtac gtaagtacgg acctgctcag
    24421 cgaacagttt catatagtcg gtagatgcca ctgccggcgc gtcgttcatc acctgagcga
    24481 tatacggaac gcgcggagtt tccagcggat gcagcatgtt ccagcgttca caatcctgac
    24541 catcacgcgc cagctcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat
    24601 ctttcgccag gatctctgcc gcttcacgga cgtgacgcag gatggaaccg gagcccagca
    24661 gctgaacttt acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac
    24721 cttcctcagc accttccggc attgccggca tgtggtagtt ttcgttcagc gtagtgatgt
    24781 agtagtaaac gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcataatga
    24841 cagcaacttc gtaagcgtaa gccgggtcgt aagagataca gttcgggata gtcagcgact
    24901 gaatgtggct gtgaccatct tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg
    24961 aagtaccgcc gatcaggaag ccacgcgctt gctggtctcc agccgcccag cacagatcgc
    25021 cgatacgctg gaagccgaac atcgagtaat agatgtagaa tgggatcatc ggcagattgt
    25081 tcgtgctgta agaggtcgcc gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc
    25141 cttcctgcag aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct
    25201 gcggggtgta ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca
    25261 taccgaaagt acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt
    25321 tcttcagcat cacgttcaga gcacgaacga aagcgatagt ggtagagagc tctttgctct
    25381 gctcttccag cagcgcgccg aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt
    25441 tcggctgacg gcttggcaga taaccgtgca gtttctgacg ctgagcgtgc agataggtat
    25501 gctcttcaga accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca
    25561 ccggcacatt gaaacggtcg cggatgtgac gcacgccgtc catgttcatt ttcttaacct
    25621 ggtgcgcgat gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag
    25681 caaggattac tgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt
    25741 tcttcggatc gtgaccacca cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa
    25801 ccagtgctgc ggtttcagga tatttaccga agaagtgttc acgaacgtac gcaccatctt
    25861 tcgatttgaa ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac
    25921 cgctggtatc tttacgcagc agttcatccc aacggctacc ccacatcact ttgatcacgt
    25981 tccagccagc accttcgaag atgccttcca gttcgttgat gatcttgccg ttaccggtga
    26041 ccgggccgtc aagacgctgc aggttacagt tgataacgaa aaccaggtta tccagttttt
    26101 cacgggtagc gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga
    26161 ggaacgcgta aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca
    26221 ggaatttagc ctggtaaata gcaccaatcg gacccagacc catagatacg gtcgggaact
    26281 gccagaattc cggcatcagt ttcgggtgcg gataggaaga gaggccattg ccgtgaactt
    26341 cctgacggaa gttatccagc tgctcctgag tcagacgacc ttccaggaaa gcacgggcgt
    26401 aaacgcccgg ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc
    26461 gtgcacggaa gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg
    26521 ccatgtggcc gcccagttcg aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt
    26581 tccagcggat agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt
    26641 gttcttcaac ggggatggtg ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta
    26701 caccgccttt gcgggcttca gcaagcagtt ggtcgatcag atactgagca cgctcaacac
    26761 cttcttcacg gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca
    26821 cgtcatttgg gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt
    26881 tctggaacct gtcccattga actctcgcca gaaagctcaa taagacaggt tctacgttta
    26941 gttaccgcgc ttttatatgc gcttgattta caacatcttc tggataattt ttaccagaaa
    27001 aatcactaat tctttcgttg ctccagacga cgcagagaac gctcacggcg gctctcttca
    27061 cggcttctgt cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc
    27121 gcttcttccg gcttaccgac cataatcgct tcaaatatgc gggtgcggtg actactcacc
    27181 agcggcagca tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc
    27241 aacatcggct ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcggtg
    27301 acggcaatct gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc
    27361 gccagctcta tggcgtggtg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc
    27421 agcgcggcgt aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac
    27481 tgtgattcag gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg
    27541 ctgctctgga caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga
    27601 cgttgaatcg cctcacgcaa ggagggacgg gagacgtcga actgttttgc cagttcgcgt
    27661 tccggtggga gtttttcgcc cgggcggaga gtgccttcga ggatcaaaaa ctccagttgc
    27721 tgctcaatca catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt
    27781 cttaagccac ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta
    27841 acaaagtatt caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt
    27901 ttaacaactt gacatatata acgtttcaaa gttgtaacta tgcacaaatg tagactttac
    27961 gtaggaaagg gattttttaa cgattattaa actataaaaa tccgaattga accgattcac
    28021 ttaccaattt tttgattttt aagtcaataa aaacgaattt aaattcactc tgcatattga
    28081 gaggggttga ggctgagctt tacaaacggt ttctttttaa gcaactcatc ttcaaccatg
    28141 cataaagcgg gtgcattcgc tgccgcatac cattattctt gatctgacgg aagtcttttt
    28201 gtaacaattc aaacctcctt gatgtaaaca aatcaataca acaaacggaa ttgcaaactt
    28261 acacacgcat cactgcgtag atcaaaaaaa caatcaccgc acgaggtttc atgatggaag
    28321 gtcaacagca cggcgagcag ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg
    28381 cgctgggtgg cgcgataggg acagggctat tcctaggtag cgcctccgta atacagtccg
    28441 cagggccagg gattatcctg ggttacgcca ttgctggttt tatcgccttt ctgatcatgc
    28501 gtcagctggg tgaaatggtg gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt
    28561 ataaatactg gggcagtttt gccggtttcg cctctggctg gaactactgg gtactgtacg
    28621 ttttagttgc catggcagag ctgactgccg tgggtaaata cattcagttc tggtatccgg
    28681 aaatcccaac ctgggtttct gccgccgtat tctttgtggt gattaacgcc atcaacctga
    28741 ccaacgttaa agtgtttggc gagatggagt tctggtttgc cattatcaaa gttattgcgg
    28801 tagtagcgat gatcatcttc ggcggctggc tgctgttcag tggcaacggc ggcccgcagg
    28861 cgagcgttag caacctgtgg gatcaaggtg gtttcctgcc gcacggcttc accggactgg
    28921 tgatgatgat ggcgattatc atgttctcgt tcggtggtct ggaactggtg gggatcaccg
    28981 cagcagaagc tgataacccg gagcaaagta tcccgaaagc aactaaccag gttatctacc
    29041 gcatcctgat tttctatatt ggttcgttag ccgttctgct ctcactgatg ccgtggaccc
    29101 gcgttaccgc cgataccagt ccgtttgtgc tgatcttcca cgagttaggc gatacctttg
    29161 tggcgaatgc gctgaacatc gtggtactga ctgcggcgct ctccgtgtac aacagctgcg
    29221 tatattgcaa cagccgtatg ctgtttggtc tggcacaaca ggggaacgcg ccaaaagcgc
    29281 tggcgtctgt cgataaacgc ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta
    29341 cagcattgtg cgtattgatt aactatcttg ctccggaatc cgcatttggc ctgttaatgg
    29401 cactggtggt atccgcactg gtgatcaact gggcgatgat cagtctggcg catatgaagt
    29461 tccgtcgcgc caagcaggaa caaggcgtgg taactcgctt ccctgctctg ctttatccgc
    29521 tgggtaactg gatctgcctg ctgtttatgg cggcggtact ggtgattatg ctgatgaccc
    29581 caggaatggc gatttcggta tacctgatcc cggtatggct gatcgtgtta ggtatcggct
    29641 atctgtttaa agagaaaacc gccaaagccg taaaagcgca ttaatctctc tacgccctca
    29701 cccgtacagg gtgagggcaa taatctctac accaacgccc cgtaaatcgt cagtagcgca
    29761 atcaccacca cgaccacgaa cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc
    29821 tccaccttat cgacatgcgg ttcacgcgcc agagagaact gcgccagacg cgttaatacc
    29881 tgatattgcg aagtatggaa atcacccagc gaagcaaacc aggccggtaa cgctttctca
    29941 ccatgaccga tcaaggcata taccacaccc gcaagacgaa ccggcaccca atccagtaca
    30001 tgaagcactg catcaatgcc ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc
    30061 cagtattgcc atgcacgcaa gaacgcatac cccatcagcg taacgggtcc ccaggttccc
    30121 cccacaatca gccagaacag cggtgcaaga taaaaacgaa agttaatcca cagcaatgca
    30181 ttttgcagct cacgcaaata ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg
    30241 gtgagttcgc cagccatcgt ggcacgggca tggctatcat tacgtgaagc agctgtcaga
    30301 taagcatgat aatgaagacg aactttacct gcgccaatac acagcaaacc aatcagcagc
    30361 cacaccagca gcgtgggaac gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa
    30421 gtcacaccca tcgcaataat ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc
    30481 cgccgaaaga acgcttcaag acgatgatca agctgccagt gctcgcccaa cttaaacagg
    30541 cgctcgaaaa ttaacaccag taaggttgta aatagcgtca tgttgtctcc ttgctgacca
    30601 gcgcacgaaa ccgtgcccaa tcaaatgcag gaccgggatc ggttttccga tccggcgcaa
    30661 tatcacaatg gcccgtcatg ttattagcga tatcgggata gcgatctatc agtgcccgcg
    30721 taaccgccgc aagctgttga tactgcgcat cggtatacgc cagagtatcg gtgccttcaa
    30781 gctcaatccc aatagaaaaa tcattgcagc gttcacgccc ctgatactga gagactcccg
    30841 catgccaggc acgtttatcg aaaggaacat actggactat ttcaccatca cggcgaatca
    30901 aacagtgagc ggagacgcgc aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat
    30961 caatagttcc agtgaataat gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc
    31021 taatattgtg caccaccagc agggtgggtg tttcgtcatc caggcggcaa tcgtaatgtg
    31081 gtgagggaac gcggcgcgcg ccaaccagcc acccctgttc taacaacatg caggatctcc
    31141 ttatatgtgg tgctaatacc cggttcagag tagcatgttt ctaccttatg attcgttagc
    31201 tatctggagt tttaacatgc cgcctcgccg ctacaaccct gacacccgac gtgacgagct
    31261 gctggaacgc attaatctcg atatccccgg cgcggtggcc caggcgctgc gggaagattt
    31321 aggcggaaca gtcgatgcca acaatgacat tacggcaaaa cttttaccgg aaaattctcg
    31381 ctctcatgcc acggtgatca cccgcgagaa tggcgtattt tgtggcaaac gctgggttga
    31441 agaggtgttt attcaactgg caggcgacga tgtcaccata atctggcatg tggatgacgg
    31501 cgatgtcatc aacgccaatc aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt
    31561 aacgggcgaa cgcactgcgc ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt
    31621 acgccactat gtcgaattac tggaaggcac caacacgcag ttgttggata cgcgcaaaac
    31681 cttacctggc ctgcgttcag ctctgaaata cgcggtactt tgcggcggcg gagcgaatca
    31741 ccgtctgggg ctttctgatg ccttcctgat caaagaaaac catattattg cctccggctc
    31801 agtgcgccag gcggtcgaaa aagcgtcctg gctgcatccg gatgcgccag tagaagtcga
    31861 agtagagaat ctggaagaac ttgatgaggc cctgaaagcc ggagccgata tcatcatgct
    31921 ggataacttc gaaacagaac agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct
    31981 actggaagtg tctggcaacg tcactgacaa aacactgcgc gaatttgccg aaacgggcgt
    32041 ggactttatc tccgtcggtg cgctaactaa acacgtacaa gcactcgacc tttcaatgcg
    32101 ttttcgctaa ttgctgacag atttatccgc tccatcgcgg gcggataatc tccccttccc
    32161 caactttctt cgtaacgcct cgcaaatttc ttgtctttcg ctgcaacgaa ataaaaaacc
    32221 tcggaatctg ctttcccgtt tcgttttttg cccctcgcct gtgctgatgg cgtttgccaa
    32281 agtagcacca accaaatcaa ggagcgaaac agatggacaa gcaacgcggt tttacactta
    32341 tcgaactgat ggtggttatt ggcatcattg ccattttaag ctccattggt attcccgctt
    32401 atcaaaacta cctgcgcaaa gccgcactca ccgacatgct acaaaccttt gtgccttacc
    32461 gtaccgccgt agagttgtgc gcgctggaac atggtggatt agatacctgc gacggtggca
    32521 gcaatggcat tccctcgcct accaccaccc gctatgtttc agccatgagt gtggcaaagg
    32581 gcgtggtgtc gctgaccggg caagaaagtc tcaatgggct aagcgtcgtc atgacgccag
    32641 gttgggataa cgcaaacggc gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca
    32701 gcgcattgca gcaagcctgc gaagatgtct tccgctttga tgacgccaac taaggagcgg
    32761 caatgaatat tccacagctc acggccctgt gcctgcgtta tcagggagtc ttgctggatg
    32821 ccagcgaaga agtggttcat gttgcggtgg tcgatgcccc ctcacatgag ttgctggacg
    32881 cattgcattt cgctaccacc aaacgtattg agatcacctg ctggacgcgc caacaaatgg
    32941 aaggtcacgc cagtcgcaca caacagacat tacccgtagc tgttcaggag aagcatcagc
    33001 ccaaagcaga gttgctgact cgaacgttac aatctgcgct ggaacaacgc gcgtctgata
    33061 ttcatatcga accagcggac aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc
    33121 atcctttacc ggatgtttca ccggatgccg gagtcgcatt aacagccaga ttaaaagtgc
    33181 tgggaaacct ggatattgcg gaacatcgcc tgccgcagga cgggcaattc actgtcgaac
    33241 tggcaggaaa caccgtctca tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg
    33301 tggtattaag gttgttacag caggtgagcc aggcactgga tgttaacacg ctgggaatgc
    33361 agccgttaca actggcggac tttgctcatg ccttgcaaca accacaggga ctggtgctgg
    33421 taactggccc tacaggcagt ggcaaaacgg tcacctggtg ttgtccacgc tacacaccaa
    33481 ttccaccact gaaacgctgg tacgtttaca gcaaatgggg gtcgcccgct ggatgctatc
    33541 atcggcgctt acgctggtaa tagcccagcg tctggtacgt aaactttgcc cacattgtcg
    33601 ccgacagcaa ggggagccca tccatattcc agacactgta tggccgtcgc cgctgcccca
    33661 ctggcaggca cccggttgtg tacattgcta ccacggtttt tatggtcgta ccgccttgtt
    33721 tgaagtcctg cccattacac cagccatacg ccagcttatt tccgctaata ctgaagttga
    33781 atcgctggaa acgcacgccc gacaggcggg tatgcgtacg ctttttgaaa acggctgcct
    33841 ggccgtggag caaggcttaa ccacctttga agagttaatc cgcgtactgg ggatgccgca
    33901 tggcaagtaa gcaactctgg cgctggcatg gcataaccgg cgacggcaat gcgcaagatg
    33961 ggatgctatg ggcagagagc cgtactttac tgcttatggc actacagcaa cagatggtta
    34021 ccccactaag cctgaagcga atcgccatca attctgcgca gtggcgagga gataaaagcg
    34081 cggaagtcat tcatcaactg gcgacgctac ttaaagccgg gttaacgctt tctgaagggc
    34141 tggctctgct ggcggaacag catcccagta agcaatggca agcgttgctg caatcgctgg
    34201 cgcacgatct cgaacagggc attgcttttt ccaatgcctt attaccctgg tcagaggcat
    34261 ttccgccgct ctatcaggcg atgatccgca cgggtgaact gaccggtaag ctggatgaat
    34321 gctgctttga actggcgcgt cagcaaaaat cccagcgtca gttgaccgac aaagtgaaat
    34381 cagcgttgcg ttatcccatc atcattttag cgatggcaat catggtggtt gtggcaatgc
    34441 tgcattttgt tctgccggag tttgccgcta tctataagac cttcaacacc ccactaccgg
    34501 cactaacgca ggggatcatg acgctggcag actttagtgg cgaatggggc tggctgctgg
    34561 tgttgttcgg ctttctgctg gcgatagcca ataagttgct gatgcgccgc ccgacctggc
    34621 ttatcgcgcg gcagaaattg ctgttacgca tcccgattat gggttcactg atgaggggac
    34681 aaaaactcac gcagatcttt acgattctgg cgctgacaca aagtgcaggc attacttttt
    34741 tacagggcgt agagagcgtc agagaaacaa tgcgctgccc gtactgggtg caacttctga
    34801 cacaaatcca gcacgatatc agtaacggtc atcccatctg gctggcgcta aaaaatgccg
    34861 gtgagtttag cccgctctgt ttgcaattag tgagaacagg agaggcatcc ggctcgctgg
    34921 acctcatgtt agacaacctc gcccatcatc atcgggataa cacaatggcg ctggcggata
    34981 acctcgcagc cttactggaa ccggcgttgc tgatcataac gggaggaatt atcggtacgc
    35041 tggtggtggc aatgtatctg ccaattttcc atttaggcga tgcgatgagt gggatgggat
    35101 aacgctggcg tgttgctcca cgccagcgtt gggagattac aggttgttga agatgcggtt
    35161 ttcttgttcc agcacacgaa taaacgtggt gcgcttggtc agctctttca ggcgtgaagc
    35221 cccaacgtat gtacaagctg aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac
    35281 ctggcctcgc agcggcagct taacggtttt accttctgct gcgcggtatt ccgcaacgcc
    35341 gccaacgtga cgtttcatcg cagactcgga gctcatgccg tagaacagca taaatttctc
    35401 gccgttctcc tcaacgatgc gaccgccgct ctcttcgtgg cccgccagca tgccgccaag
    35461 catgacgaaa tcggcaccgc cgccaaaggc tttcgccaca tcgcccggcg tggtacagcc
    35521 accgtcgctg acgatcattc cgcccagacc gtgcgccgca tcggcacatt cgattaccgc
    35581 agaaagctgc ggataaccga cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc
    35641 aatgccaact ttaacgatat cagcaccgga gaggataagc tcctcacaca tttcaccagt
    35701 cactacgttg ccagcacaaa tggttttggt cggccacgct tcacgcgctt tcgcaacgaa
    35761 ctgcacgaag tgttcggaat aaccattcgc cacatcaata caaacgaagt ttaatgccgg
    35821 gttcaggtcg agaatctgtt tagttttttc gaaatccgca tcagacgtgc cggtagaaac
    35881 catcacatgt ttcagcacat cagcggaaga attgttgata aacgcttgcc actcttcgac
    35941 agaaaagtgt ttatgcacag cagtcagaat atcaaaagaa gccagcgcag aggccataga
    36001 aaatgtgcct acggtgtcca tatttgcggc gataatcggc acgccggacc agctctgacc
    36061 tgaatgtttg aaggtgaatt gacgttccag ttcaacatcg gaacggcttt taagagtgga
    36121 gcgtttaggg cggatgagaa cgtctttaaa acctaacttc agatcttctt caatacgcat
    36181 gtgcggattc ctggggttaa tggctataag ggtaagagcg caactccagt gacgctatca
    36241 tacgcactaa tcaatgcggc gcaagactgc gaaattgtct ttttttacgc tacaatccca
    36301 taaatttacc tggtaaatag tgagcgaacg tttcatcaat ttcactttcg cgcgctcatt
    36361 tttaacgttt tgataatcat gatgaattcc cgggaatata aattatgggg tatatagttg
    36421 ccttaacggg aggcattggc agtggtaaga gtaccgttgc caatgcgttt gctgatctcg
    36481 gaattaacgt cattgatgcc gatattattg cgcgtcaggt ggttgaacca ggtgcacctg
    36541 cgctacatgc cattgctgat cactttggcg aaaacatgat tgctgctgat ggaacattgc
    36601 agcgccgggc cttgcgcgag cggatcttcg ccaacccgga agagaaaaac tggcttaacg
    36661 acctgctgca tccgctgatt cagcaagaga cgcaacacca gatccagcaa gcaacttccc
    36721 cctatgtact gtgggttgtg ccattgctgg tagaaaactc actgtataaa aaagcgaatc
    36781 gagtgctggt ggtggatgtc agcccagaaa cgcaacttaa gcgcaccatg cagcgcgatg
    36841 atgtaactcg cgaacatgtc gaacaaatcc ttgctgctca ggcaacgcgc gaagcccgcc
    36901 ttgccgtggc agatgacgtc attgataata acggcgcacc ggatgctatc gcatcggatg
    36961 ttgcccgcct gcacgcacac tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac
    37021 cgtaatgcag acccaggtcc tttttgaaca tccactaaat gaaaaaatgc gtacatggct
    37081 gcgcattgag tttttgattc agcaactcac cgttaattta cccatcgttg accacgctgg
    37141 cgcgctgcat ttcttccgta atgtcagtga attactggat gttttcgagc gcggcgaagt
    37201 ccgcactgag ctgttgaaag aacttaaccg gcagcaacgt aaactccaga cctggattgg
    37261 cgtgcctggc gtggaccaga gccgtattga agcattaatt cagcagttaa aagcggcggt
    37321 gagcgtatta atttccgcgc cgcgtatcgg gcaatttctg cgtgaagatc gtttgattgc
    37381 tctggtgcgt cagcgactga gcatcccagg cggctgttgc agctttgatt tacctacatt
    37441 gcacatttgg ctgcatctac cccaggcgca gcgcgacagc caggtagaaa cctggattgc
    37501 cagcctgaac ccgctcaccc aggcacttac catggtgctg gatttaattc gccagtcggc
    37561 ccccttccgt aaacaaacca gcctgaatgg tttttatcag gataacggtg gcgatgccga
    37621 cttgctgcgc ctgaatctgt cgctcgattc acagctttat ccgcaaattt ccggacataa
    37681 gagccgtttt gccattcgtt ttatgccgct ggacactgaa aacggacagg taccggaacg
    37741 tctggatttc gaactggcct gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa
    37801 ttgcccaacc tgcgggaaaa cggtggtgtg gggtgaaatc agcccgtttc ggccattttg
    37861 ctccaaacgt tgtcagctga tcgacctcgg agaatgggct gctgaagaaa aacgaatccc
    37921 cagcagtggc gatctttccg aaagcgatga ctggagcgaa gagccaaagc agtgacattt
    37981 gtctgatgcc tcacgtaggc ctgataagac gcggacagcg tcgcatcagg catcttgtgc
    38041 aaatgtcgga tgcggcgtga acgccttatc cgacctacag acgcttaagc ttcgcaatta
    38101 ccggttcatt ggctggcgga aaatcatcgg cattaagacc gaccagcgac atccactcac
    38161 cgggttgccc ttctttaccc cacggcaccc cttcccaact ttcgaccagc cagaaccaca
    38221 gagttatatg cctgtccggg aattcatatt ccagtttttc aaatagcgaa aaatgttgtg
    38281 gggtaatccc gacctcttcc tgaagttcac gcaccaccgc ctgttccggc gtttcgccca
    38341 tttcaatttt accgccggga aactccagtt tattcgccat gtgcgcatct gctgcgcgac
    38401 gtgttataaa gatttcattg ttctcgttgc gaataatacc taccgcaatt tgcagctttt
    38461 tcattgtctt aaacctataa aaaaggcgca gaatcctgcg ccttttactt caactgttag
    38521 cttttattgc aggcgaccat ggcactgctt gtatttttta ccagaaccgc acgggcaagg
    38581 atcgttacgt cctactttgc gttcaccggt ttgcgccgcc agtgcagctg cggctgcaga
    38641 gtcgtcatcc tgatggctaa gctgctgcat ttgtgctaaa cgctcggctt ccatacgacg
    38701 ctgttgttcc agctcctcaa cctcttcagg catacgtacc tgaactttgc tcagcgtact
    38761 gataacttca tatttcaacg actccagcat cgctgcaaac atggagaacg attcacgttt
    38821 gtattcctgc ttcggatctt tctgtgcata gccacgcagg tggataccct gacgcagata
    38881 gtccatcgct gccaggtgct ctttccacag ggagtcaagc gtttgcagca tgacgccttt
    38941 ctcgaagtga cgcatcatct cagcaccaac cacttcttct ttacgctgat acacttcgat
    39001 ggactgcgcc agaatgcgct cacgcagcgt ctcttcatgc agttctggtt ctttatccag
    39061 ccactcggta attggtaaat cgaggtcgaa atcgttcttc agacgttcct gcagccccgg
    39121 aatatcccac atttcttcca gcgactgtgg cggaatgtag gcatcaatgg tggctttgaa
    39181 cacatcttca cgaatgctgt tgatggtttc gctcacatcg ctgacatcca gcagttcgtt
    39241 acgctgggag taaatggcgc gacgctgatc gttagccacg tcatcatatt ccagcagttg
    39301 cttacgaatg tcgaagttac gactttcaac tttacgctgg gcgttggcaa tcgctttagt
    39361 cacccacggg tgttcaatgg cttcgcctgg cttcataccc agtttacgca tcatgccgga
    39421 tactcggtcg gaagcaaaaa tacgcatcag cgcatcttcc atcgacaggt agaaacggga
    39481 agaaccagca tccccctgac gaccagaacg accgcgcaac tggttatcga tacgacggga
    39541 ttcgtgacgc tcggtaccga tgatatgcag gccacctgct tccagtaccg catcgtgacg
    39601 tacctgccag tcggctttaa ttttttcaat ttgctctgcg gtcggatttt ccagcgcggc
    39661 aacttctgcc tgccagctac ctccgagcac aatatctgta ccacgacccg ccatgttggt
    39721 cgcgatagtc accgcagccg gataacctgc ctgagcaaca atcgccgctt cgttggcgtg
    39781 gaatttggcg ttcaggacat tgtgcttaat accggctttg gtcagttcgt ttgacaccag
    39841 ctccgatttt tcgatggaga tagtacccac cagcaccggc tggcctttcg cagtacgttc
    39901 tttgatatct tcaatgatcg cctgaatttt ttccgcttca gtcatgtaga ccaggtccgg
    39961 cagatcttta cgaatcattg gacggttggt cggaacaacg acggtatcca gtttgtagat
    40021 ggagctaaat tcgaaagctt cggtatcagc agtaccggtc atccccgcca gtttttcata
    40081 cagacggaag tagttctgga aggtgatcga agccagcgtt tggttttcgt tctggatctg
    40141 cacaccttct ttcgcttcca cagcctggtg cagaccatcg gaccagcgtc ggccctgcat
    40201 ggtacgaccg gtgtgttcgt caacgatgat aacttcacca tctttaacga tgtagtcgac
    40261 gtcacgggta aacagcgcat gagcgcgcag cgccgccgtt acgtggtgca tcagcatgat
    40321 gttggccgga gagtacagag actccccttc atccatgatg ccctctttca ccagcagttc
    40381 ttcaatcagc accagaccac gttcggtcag gttcacctgg cgagattttt cgtccaccga
    40441 gaagtggcct tcgccctgga aggtttcgga gtcttctttt tcctgacgga tcaggtgcgg
    40501 aataatttta ttcacgcgtt tatacatttc cgagctgtct tctgccgggc cggaaatgat
    40561 cagcggtgta cgcgcttcat cgatcaggat ggagtccact tcgtccacca gcgcatagtg
    40621 cagtttacgc tgtacacgtt cttcagggct gaacgccatg ttgtcgcgca ggtagtcaaa
    40681 gccgtattcg ttgttcgtac cgtaagtgat gtcagctgcg taagcttcgc gctttgccgg
    40741 tgctggcatg cccggcaggt tgataccaac agtcaggcca aggaattcaa acagcggacg
    40801 gttgttttcg gcgtcacgtt gcgccaggta gtcgttgacg gtaactacgt gcacgccttt
    40861 accggttagt gcgttcaggt aagcaggcag cgttgcggtc agggttttac cttcaccggt
    40921 acgcatttcg gcgatgcagc gttcgttaag aaccataccg ccgagtaact gaacgtcgaa
    40981 gtgacgcata ccaaagacgc gcttacttgc ctcacgcacc acggcgaaag cttccgggat
    41041 cagattttcc agcacttcgc ctttttccag acgcgcacga aactctgcgg ttttcccttt
    41101 cagttcttca tcggagagtt tttccatctc cggttccatg gcattgatga tgttgaccac
    41161 tttgcgcatc cggcgcaggg tgcgatcgtt acgactaccg aaaactttag ttaacaattt
    41221 gattagcata ataaaatctc aaacgccccg cgttacggag ttaataaaat gaagtaaagg
    41281 tttattgttg ttaggtgagg cgttgagggc cagcacggat gccttgcgcc tggcttatcc
    41341 agacgggcgt gctgaatttt gcttgtgggg taaaatgcgc ataatcaatg cgataaccct
    41401 tttcagacgg cgtgccttcc tgggtcagca gcgcgctgag cgtatccagt aatgcaagat
    41461 gttgcgcctg aagaggcaaa gattcttcag caacgggcag tgtttgcggt gccattgcga
    41521 aagaaagatg acggattacc gtacgaatag catgttgatg ccagtaatca acggaatagt
    41581 tcgaattcgg gcggcgcgtg ttcgcttcca gcaaggccaa ttgaccaaag ttaactttgg
    41641 ctgaaggctc gtggttgcgg gttgtcgctt ttgcgggcgc gtttggttcg gcggcgttgc
    41701 tgagcgcagg caaacctaaa ctcgccgcga ccatccctaa taagagatgc ggccagaagt
    41761 agcgtttacc aaactgtcgc cagcgcgtca gtattccact cacgttattg ccatcccgat
    41821 cgaattcaaa cgtatttagc gcacaaatct tatcattaaa gcctgaagtc cacagcacga
    41881 atgaagataa gttgtgcgta aaagtgctta agaaaacagc gttcgcaccg ttttttatag
    41941 ctcttataaa attcaaaaaa aaacgactgg ttcacctggc cggagagagt gccagatttg
    42001 ccagtcgaat tttatacgac agtataaatg tcgttatgcc agtacagctg aaggcgcttt
    42061 gaaggccaac ggcagttctg cgtcgtcctg gaaggtcaca tattcccagg cttcctgttt
    42121 cgccaggaca gcctgcagca gtttgttatt cagtgcatga ccggatttat aagcggtaaa
    42181 tgcaccaata atattgtgac cacacatgaa caagtcaccg atcgcatcga gcattttgtg
    42241 acgcacaaat tcgtcttcaa aacgcaggcc gtcttcgttc agtacgcgat aatcgtcaac
    42301 aacgatggca caatcgaagc tgccgcccag gcacaaacca cgggactgca gatattcgat
    42361 atcacgcatg aaaccgaacg tacgcgcacg gctgatctgg cgcataaacg catcagcgga
    42421 gaagttcatc gcatagcgct ggttgctgga atcaatagcc ggatggttaa aatcgatggt
    42481 gaaatccagc gaaaaaccat tgtacggctt aaattcagcc cacttatcgc catcttcgac
    42541 acgaacagtc tctttgatgc gaacaaattt tttggcgcag ttcaactcgt cgataccggc
    42601 gtcaagcagc aggtatacaa acggagcggc gctgccgtcc atgatcggga tttccggcgc
    42661 gttaacttcg ataacaatgt tatcgatgcc caggcccgcg agagcagcat tgaggtgctc
    42721 tacggttgaa atccgtacat catgctcgtt gaccagacac gtacagagca tggtatcacg
    42781 cacagatttg gcatcggccg ggaaatctac cggtggattc aagtcggtgc gacgatagat
    42841 gaccccggtg ttggccggcg cagggcgtaa cgtcagggtg actttcttgc cggtatgtaa
    42901 accgacaccc gtcgcctgaa cgatacgttt aagtgtcctt tgtttgatca tcgtattatc
    42961 tcgccaaatt acctatccaa ccgaagtgta ctatacattc ggcgggccag tttagcacaa
    43021 agagcctcga aacccaaatt ccagtcaatt cttaatcagc ttgcttacgt aggaatgctg
    43081 ggatatccag ataatccggc tctttcgcag tttgcggcgc attgtcattc actactttag
    43141 caaccggctt ctgctcctgg gtcagcggag ccatcccatg ctgctggtag cgatccatca
    43201 ctggctgctg aacctgctta ttggtcacca gagtgatttc aggacgtttg tccatgccga
    43261 tacctgtcgc aacaacggtt acgcgcagct cgtcattcat atccgggtca agagaagtac
    43321 cgataaccac agtcgcgttg tcggaagcaa atgcacggat ggtgttacct accgtttcga
    43381 actcatccag acgcaggtcg aagcccgccg tgatgttaac cagcacgccg cgcgcgccag
    43441 acaggtcgat atcttccagc agcggagaag agatagccat ttcagcagct tcttccgcac
    43501 ggtcttcacc gctcgccacg ccagaaccca tcattgcgta gcccatttca gacattacgg
    43561 tgcgtacgtc tgcaaagtcc acgttcatca aacccggacg agtaatcagt tcggcgatac
    43621 cttgcacagc gcctttcagt acatcgttcg ctgcgccaaa cgcatccagc agggagatac
    43681 cgcggcccag aactttcagc agtttgtcgt tcgggatagt gatcagagag tccacatgct
    43741 tggacagttc agtgatcccc tgctcggcga atgccatacg cttcttgcct tcaaagttga
    43801 aaggcttagt gacgacagca acggtcagga tacccaaatc ttttgccact tcagcgacga
    43861 ctggtgctgc acctgtaccg gtaccaccac ccatacccgc agcaataaag accatgtctg
    43921 caccttccag cgccgcacgc aatgcatcgc gatcctcatc agccgcattg cggccaactt
    43981 ctggattagc gccagcgccc agtcctttgg tgataccgct accgatttga atcgtctgtc
    44041 caaccgctgt tttacgcagc gcttgtgcat cggtatttac cgcgaagaat tcaacacctt
    44101 caatacgctc gcgcaccatg tgttcaacag cattaccgcc gccgccgccg acgccgatga
    44161 ctttaatcac cgcgtcattg gtaagttcca ttggttcaaa catagtttct ctccgatttg
    44221 tgcctgtcgc ctgagtccgt aatcatcgtc ggcctcataa aaattaaaac tcttttcgca
    44281 gccaactatt gagtcgcttg atccacgagc caactgatgc tgtaacacgt ttttctactt
    44341 cagcttcacc gttaagatgt gactctttcc catagtgaag caatcccacc gccgtcgaat
    44401 aatacggctc ctgagcataa tccgttaaac cggtaatgtt cagcggcgcg ccgatacgca
    44461 cttgcgtatg aaacacgcgc tgagcacagg ctgcaagacc ttcgatctgc gctgcgccac
    44521 cggttaatac aatgcctgcc gccaggtgat gtttaacccc ttgttggcga agcttttcct
    44581 gcaactgcaa tatctcttcg ttgaccaggt tgagcaactc ggtatagcgc ggctcgatca
    44641 cctctgccag ggtctgacgt tgcagactcc gcggcggacg accgcctacg ctcggcactt
    44701 ccacgctctc atcttttcca acgatggaac ccagcgcaca accgtggcga actttaatcg
    44761 cttcggcgtc gcttggcggc gtgccaaagg cgtaagcgat atcactggtc acgacattgc
    44821 cagcataagg aattaccttt gtgtggcgca atgccccacc ggtataaacg gcgatatcca
    44881 ttgtaccacc accgatatcg acgacgcaga cacccagttc acgttcatct tccgtcaata
    44941 ccgaataact tgatgccagt ccggcaaata tcagttggtc aactttcagc ccacaacgtt
    45001 caaccgcttt gacgatgttt ttcgccatat cgttgtgaca tgtgatcagg tgcacttttg
    45061 cctgcatccg cacacccgaa agtcctaccg gattcttgat cccttcctga tagtcaatcg
    45121 catactcttg cgggatcaca tgcagcacac gatgctcatc gcgcacacgc accgatttcg
    45181 cggtatggac gacgttttcc acatcttctt gcgtcacttc ttcttcagaa ataggcacca
    45241 taccaatttc attctggcag ctgatgtgct taccagaaag cgccagatat accgaagaga
    45301 tctgacaatc tgccatcaat tctgcctggt caatggcgcg ttgtacgcac ttgaccacgg
    45361 attcgaggtc gttcaccccg cctttatcca taccacgtga cgggcagctg cccacgccaa
    45421 tgatattgac cataccgtcg ggcagaactt cccctactaa agcggcaacc ttcgcggtac
    45481 caatttccag tcctactacc agttttctgt ccgtcgcctt gatcattgtt gttctgcctg
    45541 tgcctgattt tgttgctgag tagattcctc tggcggcaag ggcgcccagc ctactgccgc
    45601 tccagagtca taacgcaaat caacgtagct aatccgtttg ccatcggttt gcgcctgctg
    45661 ctgtaaaacc ggataaagtt ctacaaagcg aaccaaacgt ttcatcgtat cgccccggcc
    45721 aagattgagc ttaatatcgt tattcagcgt caactgccag gaacgccgcg cggtcatcgc
    45781 cgcttccttc agagtaaatc tgtcctttgc cagcatctgc cccatttcgc gatagccctg
    45841 caacacttca ttagcgctgc cttccgggcc atacagcatt ggaagcacct gcttgctggt
    45901 gcgatctggc ggcacgctga aggtgtttcc ttccgcgtct accatgtgtt gatcattcca
    45961 ccgcgcaatc ggcacatatt caaccagatg aatcttcaat tcatcaggcc actgctttct
    46021 gacgctcacc tgcttaatcc acggcaggcg ttgttctatt tgcgtctgga tgatgttgac
    46081 atcctgggtc ataaaggtac ccggctcacc caatgccagg atcgactgcc ggatatcgtc
    46141 attacgcgtg taatggcgtt caccggtcaa caccagcttt gagagcggca ggcgttgcgc
    46201 atcttccatc cagctcaaca cgacccagcc gctcaccaac actgtcgtta aaacggtcag
    46261 caggaaaagg atccccgcca gacgcgttcc attattgcgg cgagaagaaa cctcttcttc
    46321 gctgtttcgc gtgttcagag cagcctgcga catattagtc cgccagttcc agaattcgta
    46381 ctaccaactg cgagaagctc atacctgcct gacgtgccgc catcggcacc aggctgtggc
    46441 tggtcatacc cggtgaggta ttggcttcca gcagataaaa ctgtccatca ctgtccagca
    46501 taacgtcaat acgtccccat cctttgcaac ctaacgtcgt ccatgctttc agcactaatg
    46561 cctgcaaatt ggcctcttgt gacgcttcca gacctgcggg gcagaaatac tgtgtctcat
    46621 cagagagata cttcgcctca taatcataga aggttccgga cggttgaata cgtattgacg
    46681 gtaaaatttc ttcaccgagt atcgcaaccg tgaactccgg cccacttagc catttttcaa
    46741 tcaatacttc ttcatcgtgc tgaaatgcca atcttaatgc atcttgtaga gcattttctg
    46801 ctactacttt tgacattccc acactggaac cttcgcggct cggcttaacg ataaccggca
    46861 aacccagagc agaaatttct gctaactgct tatcgctcag gcctttttca aactctgcgc
    46921 gggttaacgc tacccacggc gcgaccggta aaccggcacc ttgccacaga agtttgctgc
    46981 gtagtttatc tattgaaagc gcagatgcca tcactccgct gccggtataa ggcaagccca
    47041 tcagctcgag catgccctgc agcgtaccat cttcaccgcc gcgaccgtgt agcgcgataa
    47101 acactttctg aaaacccatc gacttcagtt gcgtcacgtc gacttctttc gggtcgacag
    47161 gatacgcatc aataccgcct tcacgcagcc cggctaacac cgctgcgcca gaattcagag
    47221 aaacttcccg ctcagcggaa gtcccgccca acaggaccgc gattttatca gtcatgttgt
    47281 tcttcctccg gagtttgcgg cttcagtttg atttcagcta aagaacgggc aatttttccg
    47341 atattaccag ccccctgaac gagaatcagg tcgttaccgg ttaataccgg tgccagcatc
    47401 tcggctacct gcgcagggtc cggcaccaga atgggatcaa ttttcccacg tccacgaatt
    47461 gtgcgacaca gcgaacggct gtccgctccc ggaattggcg cttcgccagc cggatacact
    47521 tccagcatca acagggtatc aacctgcgtc agcacattgg cgaaatcatc atacaggtcg
    47581 cgcgtacggg taaaacggtg cggctgaaac agcattacca ggtttttatc cggccagcct
    47641 gcgcgcgccg ctttaatggt ggcgtcaact tccgtcgggt ggtggccgta gtcatcgacc
    47701 agcattgccg taccgctttt accattcact ggctccagcg ggaattcacc gaggaaatca
    47761 aaacggcgac cagtcccctg gaagctctcc agcgcacgca aaatagcctc gtcgtcaatt
    47821 ccctcttccg tagcaaccgc aaccgcagct gcggcgttca gcgcgttatg acggcctggc
    47881 gcattcaggg tgacgcacat cggctctttg tcctgacgca gcagcgtaaa gtgcccctgc
    47941 gggccaatct gctgataatc ttctacacgc acgtcggcat cttcgctgaa gccgtaagtt
    48001 gtggtctgac gtcccacacg cggtaacaat tcgcggatca ccggatcatc aacacacatc
    48061 accgcacgac cgtaaaaagg caggttgtgc agaaaattaa taaaggtctg ttttaaattc
    48121 tcaaagtcgc cctggtaggt atccatgtgg tcggcttcga tattggtgac aatcgccacc
    48181 atcggttgca gatgcaggaa cgatgcatca ctctcatctg cttcggcaat caggtaccga
    48241 ccatgcccca aacgcgcatg aacccccgcc gcttttacca gcccgccgtt aacgaaggtt
    48301 gggtcgagcc ccgcttctgc gtagatgctg gaaaccatcg cggtagttgt cgttttgccg
    48361 tgcgttcctg caatggcgat gccatgacga aaacgcatta actcagccag catttcggca
    48421 cgacggatca ccggaatacg cgcttcatga gctgcgacaa tttccgggtt gtcggcagaa
    48481 atggcgctgg aaacaacgac cacgctggca tcacgtacgt tttccgggcg atggttgaaa
    48541 taaatcgtcg cacccagatt cagtaactgc tgcgtgaccg gatttggcgc taaatcggaa
    48601 ccactgatct gataaccttc attggccaga acttcggcaa taccgcccat accggcacca
    48661 ccaatgccga caaaatgtat gtgccgaacg cgacgcattt cgggcacgat ggaacgcagt
    48721 tttgccaatt gttgtgtatt cattctttac gccattaact tcttaaattc atacgatgca
    48781 aaaggcatcg ctacaattac gcccgggcag cccggctcac ttcatttgcc actcgctcgg
    48841 tggcatccgg aatggatgca gcgcgggcgc gttctgccat ggttaataag gtttctcgcg
    48901 accacccggc cagggtgttg gcgacagcat ccacgctaag ctgtggctgc tcgataattt
    48961 tggctgcgcc cgctttttcc agcggtagcg cattccagta ttgctggcgg tctttatgtt
    49021 gaaacggcac aaacaacgcc ggtagtcctg ccgcggcgat ttcactcaca gttaaagcac
    49081 cggagcggca aacgacgaca tccgcccacg catacgccgc cgccatatca tcaataaatt
    49141 cagtcacttt atgctgcggt tgccccgctt cggcatacgc ctgttcaacg gattgttgcg
    49201 aacctttgcc gctctgatgc cagatagtga ctgaatcacc cagtttcgca gcaacctgcg
    49261 gcattgtctg gttaagaatg cgtgcgccct gggaaccacc cactaccaac acacgaaccg
    49321 gaccttcacg tccagccaaa cgttgctgcg gcaacggcag cgccagcaca tcggtacgca
    49381 ccgggttacc caccacttcc gcattaggga aagcgcctgg aaacgcctgc atcactttgg
    49441 tagcaatctt cgccagccat ttattggtta agcccgcaat accgttttgt tcatgaagta
    49501 caaccggaat gcctaacgac cacgcggcca gaccacctgg acctgataca tagccgccca
    49561 taccgagtac cacgtcaggt ttgtacgctt tcataatcgc ccgcgcctga cgccaggcgt
    49621 tgaagatacg cagcggcgca gctatcagtg cttttatacc ttttccacgc aaaccagaga
    49681 tacgaatgaa atcaatttcg atgccatgtt ttggcactaa gtccgcttcc atacggtcgg
    49741 cagtccccag ccagcgaact tgccaaccct gagccattag atagtgcgca accgccagtc
    49801 ccgggaatac atgtccaccg gttccgcctg ccatcaccat taatcgcttt ccttgaccac
    49861 tcatcgtgaa cctcgtacaa acgcctgcgc tttctccaga cgcgtttcat aatcaatacg
    49921 caacagcatc atgatggctg tcgacataat cagtaagctc gaaccaccgt aactgattag
    49981 cggcaatgtc agacctttgg tcggtaacat ccccgccgcc gcgcctacgt taaccagcgc
    50041 ctggaagcta aaccagatgc caatagaaca ggcgagaaaa ccggaaaaac ggtggtcaat
    50101 ttctaatgct ttgcggccaa tcgacatcgc acgaaaagcg acgaagaata ccattaaaag
    50161 tgctagtacc acaccgacat aacctaattc ttcgccgata atggcgaaaa taaagtcagt
    50221 gtgcgcttcc ggcagatact ccagtttttg taccgaatta cctaaacctt gcccccaaag
    50281 ttcgccgcga ccaaacgcca tcagcgattg cgttaactga tagccgctgc caaagggatc
    50341 ttcccacggg ttccagaatg cggtaacacg gcggatacgg tacggttcgg cgaggatcag
    50401 caacacaacc gctgaaatgc ccataccgat aatggcaatg aactgccaca atttcgctcc
    50461 cgccaggaac aacatcgcca gcgtagtcac aaacaacacc accaccgtac caaggtctgg
    50521 ctgtgccagc agtaacactg ccaacaccag aatcacgccc atcggtttca ggaagccgcg
    50581 caggttatta cgtacttcgt cgcctttacg caccagatag ttggcgatat agcaaaacag
    50641 cgacagtttt gtcagctccg caggctggat acgcagcaaa ccgagatcga tccaacgcga
    50701 tgccccttta acagcgctac ccactaccag cacgatcatc agcaggatga tagatccgag
    50761 cagcatcgtg gcactgtagc gttgccagaa ctccatcggc agacgcagcg taatgatcgc
    50821 cagaataaac gccaaaatca gatagacacc atcacgcttc gcgaagaaga acggatcgtt
    50881 ggttaagcgt tgccctatgg gcattgacgc cgaggtcacc atgataaagc caatcgccgc
    50941 gaggccgaag gtcagccaca gtaaggtgcg atcgtacatg atcaggctgt cggtatcttt
    51001 ttcccgcgag cccatcaccc agccctttag cgccgtggag atccagacca ggatactgaa
    51061 tcctggcagg cgcggcattt tcaggcgagg gagagataaa cgcatcaacc taactccttc
    51121 gccagacggg caaactcatt gcctcgttgt tcaaagttct tgaactgatc aaggctggca
    51181 caggccgggg agagcagaac catatcgccc ggctgaacac gcggagccag caagcgcata
    51241 gcctgttcca tagtttcggt ttgttctgcc acttccgggc gtagcgccgc cagctgcgcg
    51301 ccgtcacgac cgaaacaata cagacgtacg ttatcgccat tcaggtaacg caccagtggg
    51361 ctaaagtccg ccgatttacc atcgccgccc agcaacaaat gcagcgtgcc gtctacgtgc
    51421 aggccattca gcgccgcttc cgtactgccg acgttggtcg cttttgaatc gttaatccag
    51481 cgcacaccgt tatgctccag caccacttca aagcgatgcg gcagaccggt gaaggtggtt
    51541 aacgctttca ggctgctggc gcgcggtaac cctgcggcat ctgccagcgc cagcgccacc
    51601 agcgcattgg tgtagttatg ctgcccggaa agtttcatct ctttcacatt cagcactttc
    51661 tcgcctttaa cccgcagcca ggtttcgccc tgctgatgat tcaggtgata gtcacccatg
    51721 ttgacgccaa agctgacgca gcgttcatcc gcaccgcgaa tcggcattgt taaggcatca
    51781 tcagcattaa ccacgcaaac tttcgcgttt tcgtaaatgc gcagttttgc tgcacgatac
    51841 tgttgtaaac caaacgggta gcgatccata tgatcttcag tcacgttcag aatggtcgct
    51901 gctaccgcct gtaagctgga ggtggtttcc agctggaagc tcgacagttc cagcacgtac
    51961 agttcacact catcatccag tagcatcaac gcaggcaggc caatattgcc acccacacca
    52021 acgttaaccc ccgccgcttt cgccatttca cccactagcg tggtgaccgt gcttttgccg
    52081 ttagaaccgg taatcgccac aatcggtgct tgtgcttcgc gacaaaacag ctcgatatcg
    52141 ccaacgattt cgattccggc atcagcggca gcgcttaagg atggatgcgc cagtgcaata
    52201 ccgggactgg cgacaatcag atctgccgcc atcagccatt catcattcag actgcccgtg
    52261 tggcgttcta cggcttcggg taatttatcc aggccaggcg gtgtcatacg cgtatccata
    52321 acgcgcggcg tcacaccgcg agcgaggaaa aagtccacgc aggaaagccc ggtgaggccc
    52381 aggccgataa tgacaacatt tttaccctgg taatcagcca tgattaacgt accttcagcg
    52441 ttgccagacc aatcaggacc agcatcagcg aaataatcca gaaacgcaca atgacgcgcg
    52501 gttccggcca gcctttcagt tcatagtggt gatgaatcgg tgccatgcgg aaaatacgtt
    52561 gtccgcgcag tttaaaggag ccgacctgca ggatgacaga aagcgtttct accacgaaca
    52621 cgccccccat aatcaccagc aggaattcct gacgtagcag tacggcgata atgcctaacg
    52681 caccacctaa cgccagcgaa ccgacatcgc ccataaagac ctgcgccgga taggtgttaa
    52741 accacaggaa gcccagtcct gccccgacta tcgcggtaca gacaataacc agttccccgg
    52801 cgtgtcgcag atacggtatg tgtaaatagc tggcaaagtt catgttgccg gtcgcccacg
    52861 ccaccagcgc aaaaccaccg gcgacaaata cggtcggcat aattgccaga ccgtcaagac
    52921 catcggtcag gtttaccgcg ttgccagtac ccacaatgac gaagtaagcc agcagaatgt
    52981 agaacagccc cagttgcggc atcacatctt taaagaatgg gaccaccagc tgcgttgcgg
    53041 gcgtgtcttt gccggcaagg tacaaagcga aagcaacacc cagcgcaatg accgacatcc
    53101 agaaatattt ccaacgagcg atcaaccctt tggtgtcttt acgcaccact ttgcgataat
    53161 catcaacaaa gccaataaca ccgtaaccta ccagcaccac caacacgcac cagacgtacg
    53221 gattggacgg gtaagcccac agcagcacgg agatcacaat cgctgtcagg atcataatcc
    53281 cgcccatggt cggcgtaccg cgcttgctga agtgtgattc aggaccgtcg ttacgcacta
    53341 cctgaccaaa ggaaagtttt tgcaaatgag caatcatgcg cgggcccatc cacaatgaga
    53401 tgaacagcgc ggtcagcagg ctgacgatgg cgcgaaacgt cagataggaa aagacgttaa
    53461 agccggaata atatttgacc aaatgttcgg ccagccaaac taacatgtcc cattctcctg
    53521 taaagcgcgt actacctctt ccatggcagc actacgtgaa cccttaacta aaatcgtaat
    53581 tacctgttgc tcagcaatca gtgatttaag acgcgtaatt aacgcagttt tatcagcaaa
    53641 atgttcgcca acgccgctgg cggtgctgat agcatggctt tgtttaccca tgcttaacac
    53701 gcggtcaata ccagcagctt ttgccgcctc acccacctgt acatggcagg cttcgctttc
    53761 agcgcccagt tccgccatat cgcccaccac cagcacgcgg tagcccggca tttcagccag
    53821 tacctgaaca gctgcggtca ttgaaccgac attggcgttg taggagtcgt cgagcagcaa
    53881 ctggttttct gccagttgga tggggaacag acggccagga acagctttca gatttgccag
    53941 ccccgctttg atagcatcaa gcgttgcgcc cacggacatg gagagcgcag cggctgccag
    54001 cgcattcgca atattgtgac gccccggcaa cggcagcaga acatcgacgc taccggttgg
    54061 ggtttgtagg gtaaattcca taccgtgcga ggtcacatgg atattggtgg cggtgaaatc
    54121 gctgttggcg gcattgggtg agaaacgcca cactttgcgt gagccaatta cgctctgcca
    54181 gttcagccag tcgttgttgt cggcgttcat aatggcgata ccgttttccg gcaggccgct
    54241 aaagatttca cctttcgctt tcgcgacacc cgcaagcgag ccaaaacctt ccagatgcgc
    54301 cgctgccagg ttgttgacca gcgccgcttc cgggcgagtc agactcacag tccaggcaat
    54361 ctcgccctga tggttcgcgc caagttcaat aactgcgtaa tcgtattccg gcgttaagcg
    54421 caacagcgtc atcggtacac cgatgtcgtt gttgagattg cctgccgtat aaagcgtgtt
    54481 gccgcactgg cttaaaatcg ccgccgtcat ctctttaacg gaggttttgc cggaggaccc
    54541 cgtcagagca accacgagcg ccggaacttg ctggcgaacc catgcagcca gttcaccaaa
    54601 cgccagacgc gtatccttga cgattaactg cggcaggtcg atgtccagcg gacggctaac
    54661 cagtagtgcg cctgcgccgc cagctttcgc ctggtcggca aaatcgtggg catcaaaacg
    54721 ttcacctttc agggcaacaa acaggcagcc cggcgtcagt tttcgggtat cagtggttac
    54781 agcatcaagg gtgatatctg caccttgcag ttcaccgttg agaatatcgg taagttggct
    54841 aagggttacg ctaatcatgc aatccccccc agcagacgcg ccaccgtgac gcgatcggag
    54901 tagtccagac gctgattgcc aacaatctgg taatcctcat ggcctttgcc cgcgaccagt
    54961 accacatcat tctctttagc ctgcataacg gcgcaagtca ccgcttcagc acggccttcc
    55021 atcactttgg catgtccggc atctaacatt cccgccagaa tatcgttgat gatggcacgc
    55081 ggttcttcgg tacgcgggtt atcgtccgtc accaccgcca cgtcagcaaa ctcttcggca
    55141 attgcgccca tcagtggacg cttaccttta tcgcgatcgc caccacagcc aaagacgcac
    55201 cacagcttgc ccgcacagtg caggcgcgcc gcctgtaagg ctttttccag tgcatccggc
    55261 gtatgcgcgt aatccaccac caccgtcggt ttgcctggcg cagtgaacac ttccatacgt
    55321 ccgcaaaccg gttgcagacg cgcggcggtt ttcagcagat cagccagtgg atagcccagc
    55381 gccagcagtg ttgccagcgc gagcaacagg ttgctgacgt taaaagcgcc catcagatgg
    55441 ctttcaattt cgccatcgcc ccagcttgaa ctaaagcgaa tggtcgcgcc gctgtcgtga
    55501 tagttcactt cggtcgcttt caaccagcgt ccgtgacagt tcggattgat atgatcttcc
    55561 attgataccg caaccgcgtc cggcagtttt gccagccagc ggcggcccac ttcatcgtcg
    55621 gcgttaataa tcgcctgacc gcaatgatgt tcagaataaa gcagccattt cgcagcttcg
    55681 tagtgttcca tatcaccatg ataatcaagg tgatcgcggc ttaagttggt aaagaccgac
    55741 gccggaaatt tcaatgccgc cacacggtgc tgtaccagcc cgtgggagga aacttccatt
    55801 gcgcaaaaag tcgcgccctg atccaccagc cccgccagct catgctgaac atcaactgcc
    55861 gaaccggtag tattttctgt cgggatcact ttccccagca gaccgttacc aacggtgccc
    55921 attaccgcgc tggtttcgcc aagcagttgg ctccactgcg ccagaagctg ggtcgtcgtg
    55981 gttttgccgt tggtgcccgt tacgcctacg agacgtaaat tgtcagaggg ttcatggtaa
    56041 aagcggcccg ccagtgcaga taaacgctcg ttgagctggc tgagatagat gaccggtacg
    56101 ccgtgcattt cacggatttc accgtcggtc gcctcatctt tcgcctctgc aataatggca
    56161 gccacacctt gcgctatcgc ctgcgggata tatcgacgcc cgtccgcctg atgacctact
    56221 acagctacaa agagatcgcc cgccgcagcc acacggctgt cgagtgtcat ctctcgcagt
    56281 gctcgcgaag gtgcgtctgg cacccacgga gcaagaaggt cgcgcaaatt acgatctgcc
    56341 acctgtcccc tcgccttgat taatcacaaa ttcattttta tcgcccgttg tcagcgcatc
    56401 cggctcgatg ttcatggtac gcaatacgcc gcccatgatg gcaccaaaga ccggcgcgga
    56461 aacggcgccg ccgtagtatt tacccgcctg cggatcgttg ataacaacaa ccagcgcgaa
    56521 gcgcggctga ctcgcaggcg caacgcctgc ggtataagca atatatttat tgatgtagcg
    56581 accgtccggc ccgacctttt tcgcggtacc ggttttaatg gcgatacgat agcctttaat
    56641 cgccgccttc acgccgccgc cgcctggtag cgccacgctt tccatcatat gcaccacggt
    56701 gcgaacaatg gattccggga agacacgttc accgggaacc ggggggtcaa ctttggtaat
    56761 cgacagtggg cgataaatgc cgtagctgcc gatagttgcg tagactcgcg ctaactgtaa
    56821 cggtgttacc attagcccgt agccgaaaga gaaggtggcc ctctctatgt cagaccaccg
    56881 ttgtttttga ggatataagc cactgcgttc tccgaccaac cccaaattgg tcgcttttcc
    56941 cagtccaaaa cgtgagtaag tatctactaa cgctgaggac ggcatcgtta acgccagctt
    57001 ggaaacaccg acgttactcg acttctgtaa taccccggtc agggttaatt cgctgtagcg
    57061 tgccacgtct ttgatttcgt ggccgttaat tcgataagga atggtattga gtaccgagtt
    57121 ttcccgcacc acgccacgtt gcaacgcggt catcaccacc atcggtttaa ccgttgagcc
    57181 cggttcaaac acgtcggtga tggtacggtt acgcatcgcc tctttcggcg tgccgctcag
    57241 attgttaggg ttgtatgacg ggctgttagc catcgccagc acttcacctg tgttgacatc
    57301 caccagcacg gcgctaccag attcagcctt gttaaaggcc accgcgttgt tcagttcgcg
    57361 ataaaccagc gcctgcaggc gttcatcaat actcagcgcc aggttgtgcg ctgcctggct
    57421 gtcagtagaa gaaatatctt caattacgcg accatagcgg tctttacgca caatgcgctc
    57481 acccggctgc ccggtaagcc atttatcgaa actcttctca acgccctcaa tcccttgact
    57541 atcgacgtta gtaaagccga tgaggtgagc agtcacttcg ccggacggat agtaacggcg
    57601 agactcttca cgcagatgaa tccccggcag tttcagtttt ttgatgtagt ccgccatgtc
    57661 agagtttacc tgacgcgcca gataaataaa gcgccctttc gggttggcgt taatgcgggc
    57721 agaaagctga tccagcggaa tattgagcgc gttagccagc gccttccagc ggtcaccgac
    57781 gctgataccg ccagcgtcat gcacttcttt cgggtcagcc caaatcgctt ttaccggcac
    57841 gctcaccgct aacgggcgac cagaacggtc agtaatcatg ccgcgggagg tggaaacttg
    57901 ctgaacgcga agagaacgca tgtcgccctc tttcaccagc atatccgggg agataacttg
    57961 taaccacgct acgcgtccga gcagaaaagc cagcgccagg agaatacagc cgcataacaa
    58021 cgcaaaacgc caactgataa agttggcatg ttcttcctga cgttttggtt tctgcgtttt
    58081 cgccgctgct ttcatgcgtc gcgtttatcc ttatttttgc actacgatat tttcttgtga
    58141 cggatcaaca tgctgcattt gcagcttttc cgtggcgatc ctttccaccc ggctatggtc
    58201 gccgagcgca ttctcttcaa ggatcaggtt gcgccattca atgtctaaag catctcgctc
    58261 cagcaccagt tgttcgcgct gagcggtcag taaacgggta tggtgcgccg tggttaccac
    58321 ggtcaccgcc gtcaaaataa tgcaaatgaa caggcagagt ggcagcttcc caaatcgcaa
    58381 aagatcgtca ccgataacac caggcaatgc atggcgctcg tggcttccca tcgatccttt
    58441 aactttgctt agagcttctg tcactctgct gatcatgcgt tcgtcctctc tgcaatacgc
    58501 agaactgaac tacgggcacg agggttctca gccacctctt cttcgcccgg cattaacttg
    58561 cctagtgctc gcagctgacg gccacccagt tttttgagct gctcttcagt catcggtaac
    58621 cctgccggaa cttgcggacc gcggctgttt tcacgcataa aacgtttcac aatacggtct
    58681 tccagcgagt ggaagctgat gatcgaaagc cgcccacccg gggccagcac gttgagcgag
    58741 ctttttagcg cctgctctat ctcctccagt tcactgttta cccaaatgcg caccgcctgg
    58801 aaggtacggg tcgcgggatg tttaaactta tctttcaccg gcgttgcagc agccacgact
    58861 tccgccagtt ctttggtgcg ggtcatcggc tgttcgcggt tacgctcgac aatggcgcgg
    58921 gcaatgcgtt tggcaaaatg ctcttcacca taggttttca atacccaggc gatatcggct
    58981 tcttctgcgg tttgtagcca ttcagcggct gactgcccac gggttgggtc catacgcatg
    59041 tccagcggac catcgcgcat aaaggaaaag ccgcgttcag catcatcaag ttgcggtgaa
    59101 gagacgccaa gatcgaggag aatgccgtcg atcttgccaa taagatcgcg ctcggcaacg
    59161 tattcgccca gcgcggagaa aggtccgtgg atgatggaga agcgcggatc atcaatagtc
    59221 ttcgcaacgg cgatagcctg cgggtcgcga tcgatcgcca gcaaacgccc ctcttcgcca
    59281 agctgcgaga ggatcagacg tgagtgacca ccgcgaccaa aagtcccatc aatgtagatg
    59341 ccatcaggac ggatattgag gccattaacg gcttcatcca gcagcaccgt agtatgttta
    59401 tagttttcca tcattttata gagacaagtc ctgcagtcgc tccgataagt ctccggtagc
    59461 caactgctct gcgtcgatat cttccttgac ctgttgatgc caggttgttt catcccacag
    59521 ctcaaacttg ttgaactgtc caaccagcat cacttctttt gtcagcccgg catgttgccg
    59581 cagtactggc gcgattaaca atcgacctgc gccatccatc tgacattcgc tggcatgacc
    59641 taacagtagg cgctgcacac ggcgctcaac cgggttcatg ctcaacagac gcgataattt
    59701 ttgctcgata atttcccatt caggcagggg gtaaagcagc aggcacgggt gatgaatgtc
    59761 aatggtgcaa accatttgac cggcagcgtt ctcaagcagc tgttcccgat aacgggtagg
    59821 cactgataag cgccctttgc tgtcgagatt gactaacgtt gctccccgga acatgccagc
    59881 ctcacccctt attcccactt tgccccacaa tttcccactg aaaggagttt acggagcgga
    59941 ggaaaagctt gtcaagccag tcacaaggca taccgaggta aaaaacccga tgtttacggc
    60001 taatatcagc gttgtttatt ttacgtccaa cgtgcgaagc aaattaacgt tatgaatagc
    60061 tgtaagaaaa aatccctcat cgtcatcact gttttaaaat gactcactat tttccacagc
    60121 aaatataaag tgtcagtttg cgacgcgagc ggcattttag gacatatctt ccccggttaa
    60181 cagtccttgt tacgtctgtg tggcgcttga tgccagcggg tttgcgcaaa agcaaagaaa
    60241 agtgtttgtt aaatcggcaa ttcatcgccg catgtaacaa aataatgcaa aaacgcgctg
    60301 tcgatatcac ttaattattg tgaatttcag ctggtgagtt aattaacggg cgggtttaaa
    60361 accatttatc aataatagtc aggaatagtc ttatttactt taaggatatt gat
//
LOCUS       DAETQL010000013        59862 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.013, whole genome shotgun sequence.
ACCESSION   DAETQL010000013 DAETQL010000000
VERSION     DAETQL010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.013"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            533..1822
                     /gene="sdaC"
                     /locus_tag="K0W65_000858"
     CDS             533..1822
                     /gene="sdaC"
                     /locus_tag="K0W65_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HBI3094237.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1880..3247
                     /gene="sdaB"
                     /locus_tag="K0W65_000859"
     CDS             1880..3247
                     /gene="sdaB"
                     /locus_tag="K0W65_000859"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBI3094238.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3358..4113
                     /gene="xni"
                     /locus_tag="K0W65_000860"
     CDS             3358..4113
                     /gene="xni"
                     /locus_tag="K0W65_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBI3094239.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="K0W65_000861"
     CDS             complement(4166..5314)
                     /gene="fucO"
                     /locus_tag="K0W65_000861"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBI3094240.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="K0W65_000862"
     CDS             complement(5342..5989)
                     /gene="fucA"
                     /locus_tag="K0W65_000862"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBI3094241.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6536..7852
                     /gene="fucP"
                     /locus_tag="K0W65_000863"
     CDS             6536..7852
                     /gene="fucP"
                     /locus_tag="K0W65_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBI3094242.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7885..9660
                     /gene="fucI"
                     /locus_tag="K0W65_000864"
     CDS             7885..9660
                     /gene="fucI"
                     /locus_tag="K0W65_000864"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HBI3094243.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9769..11188
                     /gene="fucK"
                     /locus_tag="K0W65_000865"
                     /pseudo
     CDS             9769..11188
                     /gene="fucK"
                     /locus_tag="K0W65_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11190..11612
                     /gene="fucU"
                     /locus_tag="K0W65_000866"
     CDS             11190..11612
                     /gene="fucU"
                     /locus_tag="K0W65_000866"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBI3094244.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11670..12401
                     /gene="fucR"
                     /locus_tag="K0W65_000867"
     CDS             11670..12401
                     /gene="fucR"
                     /locus_tag="K0W65_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBI3094245.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="K0W65_000868"
     CDS             complement(12445..13545)
                     /gene="rlmM"
                     /locus_tag="K0W65_000868"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBI3094246.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13538..13933)
                     /locus_tag="K0W65_000869"
     CDS             complement(13538..13933)
                     /locus_tag="K0W65_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBI3094247.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="K0W65_000870"
     CDS             complement(13952..14869)
                     /gene="gcvA"
                     /locus_tag="K0W65_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HBI3094248.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15220..15447)
                     /locus_tag="K0W65_000871"
     CDS             complement(15220..15447)
                     /locus_tag="K0W65_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBI3094249.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15639..16844
                     /gene="csdA"
                     /locus_tag="K0W65_000872"
     CDS             15639..16844
                     /gene="csdA"
                     /locus_tag="K0W65_000872"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBI3094250.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16844..17287
                     /gene="csdE"
                     /locus_tag="K0W65_000873"
     CDS             16844..17287
                     /gene="csdE"
                     /locus_tag="K0W65_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBI3094251.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="K0W65_000874"
     CDS             complement(17338..18144)
                     /gene="tcdA"
                     /locus_tag="K0W65_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBI3094252.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="K0W65_000875"
     CDS             complement(18382..19479)
                     /gene="mltA"
                     /locus_tag="K0W65_000875"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBI3094253.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFVEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19688..19764
                     /locus_tag="K0W65_000876"
     tRNA            19688..19764
                     /locus_tag="K0W65_000876"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19722..19724,aa:Met,seq:cat)
     gene            19798..19874
                     /locus_tag="K0W65_000877"
     tRNA            19798..19874
                     /locus_tag="K0W65_000877"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19832..19834,aa:Met,seq:cat)
     gene            complement(19949..21202)
                     /gene="amiC"
                     /locus_tag="K0W65_000878"
     CDS             complement(19949..21202)
                     /gene="amiC"
                     /locus_tag="K0W65_000878"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HBI3094254.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21434..22765
                     /gene="argA"
                     /locus_tag="K0W65_000879"
     CDS             21434..22765
                     /gene="argA"
                     /locus_tag="K0W65_000879"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBI3094255.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22827..24653)
                     /gene="recD"
                     /locus_tag="K0W65_000880"
     CDS             complement(22827..24653)
                     /gene="recD"
                     /locus_tag="K0W65_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBI3094256.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24653..28195)
                     /gene="recB"
                     /locus_tag="K0W65_000881"
     CDS             complement(24653..28195)
                     /gene="recB"
                     /locus_tag="K0W65_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBI3094257.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28188..31076)
                     /gene="ptrA"
                     /locus_tag="K0W65_000882"
     CDS             complement(28188..31076)
                     /gene="ptrA"
                     /locus_tag="K0W65_000882"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HBI3094258.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31252..34620)
                     /gene="recC"
                     /locus_tag="K0W65_000883"
     CDS             complement(31252..34620)
                     /gene="recC"
                     /locus_tag="K0W65_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBI3094259.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34633..34983)
                     /locus_tag="K0W65_000884"
     CDS             complement(34633..34983)
                     /locus_tag="K0W65_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBI3094260.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34968..35375)
                     /locus_tag="K0W65_000885"
     CDS             complement(34968..35375)
                     /locus_tag="K0W65_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBI3094261.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35372..35935)
                     /locus_tag="K0W65_000886"
     CDS             complement(35372..35935)
                     /locus_tag="K0W65_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBI3094262.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35926..36396)
                     /locus_tag="K0W65_000887"
                     /pseudo
     CDS             complement(35926..36396)
                     /locus_tag="K0W65_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36580..37374)
                     /gene="thyA"
                     /locus_tag="K0W65_000888"
     CDS             complement(36580..37374)
                     /gene="thyA"
                     /locus_tag="K0W65_000888"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBI3094263.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37381..38256)
                     /gene="lgt"
                     /locus_tag="K0W65_000889"
     CDS             complement(37381..38256)
                     /gene="lgt"
                     /locus_tag="K0W65_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBI3094264.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38407..40653)
                     /gene="ptsP"
                     /locus_tag="K0W65_000890"
     CDS             complement(38407..40653)
                     /gene="ptsP"
                     /locus_tag="K0W65_000890"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBI3094265.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40666..41196)
                     /gene="rppH"
                     /locus_tag="K0W65_000891"
     CDS             complement(40666..41196)
                     /gene="rppH"
                     /locus_tag="K0W65_000891"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBI3094266.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41881..42570
                     /gene="mutH"
                     /locus_tag="K0W65_000892"
     CDS             41881..42570
                     /gene="mutH"
                     /locus_tag="K0W65_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBI3094267.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42639..43352
                     /locus_tag="K0W65_000893"
     CDS             42639..43352
                     /locus_tag="K0W65_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBI3094268.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43490..43708
                     /gene="ygdR"
                     /locus_tag="K0W65_000894"
     CDS             43490..43708
                     /gene="ygdR"
                     /locus_tag="K0W65_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBI3094269.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43816..44856
                     /locus_tag="K0W65_000895"
     CDS             43816..44856
                     /locus_tag="K0W65_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBI3094270.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44889..46079)
                     /gene="lplT"
                     /locus_tag="K0W65_000896"
     CDS             complement(44889..46079)
                     /gene="lplT"
                     /locus_tag="K0W65_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBI3094271.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46072..48231)
                     /gene="aas"
                     /locus_tag="K0W65_000897"
     CDS             complement(46072..48231)
                     /gene="aas"
                     /locus_tag="K0W65_000897"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBI3094272.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48817..49848
                     /gene="galR"
                     /locus_tag="K0W65_000898"
     CDS             48817..49848
                     /gene="galR"
                     /locus_tag="K0W65_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBI3094273.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49855..51117)
                     /gene="lysA"
                     /locus_tag="K0W65_000899"
     CDS             complement(49855..51117)
                     /gene="lysA"
                     /locus_tag="K0W65_000899"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBI3094274.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51239..52174
                     /gene="lysR"
                     /locus_tag="K0W65_000900"
     CDS             51239..52174
                     /gene="lysR"
                     /locus_tag="K0W65_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBI3094275.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52161..52853)
                     /gene="ygeA"
                     /locus_tag="K0W65_000901"
     CDS             complement(52161..52853)
                     /gene="ygeA"
                     /locus_tag="K0W65_000901"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBI3094276.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52982..54400)
                     /gene="araE"
                     /locus_tag="K0W65_000902"
     CDS             complement(52982..54400)
                     /gene="araE"
                     /locus_tag="K0W65_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HBI3094277.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54715..55476)
                     /gene="kduD"
                     /locus_tag="K0W65_000903"
     CDS             complement(54715..55476)
                     /gene="kduD"
                     /locus_tag="K0W65_000903"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBI3094278.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55506..56342)
                     /gene="kduI"
                     /locus_tag="K0W65_000904"
     CDS             complement(55506..56342)
                     /gene="kduI"
                     /locus_tag="K0W65_000904"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBI3094279.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56629..57810)
                     /locus_tag="K0W65_000905"
     CDS             complement(56629..57810)
                     /locus_tag="K0W65_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBI3094280.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58065..59294
                     /locus_tag="K0W65_000906"
                     /pseudo
     CDS             58065..59294
                     /locus_tag="K0W65_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT        14780 a        16062 c        14576 g        14444 t
ORIGIN      
        1 ttgccgggtt ttctcgtttt tggtcactta ctcatcaact catttcattt gttatatgaa
       61 tgtttcttac caccctcacg cgacaaatat catcacagtt aatatgtcat acaatttatg
      121 ttgcaacaca aacgtttccc tattttcata aaccgtattt tatcgctatg aaaaagaaat
      181 tatcgccatg attaactaaa agtattgatt ttttcagttc aacctacata tattgcgcgc
      241 cccggaagaa gtcagatgtc gtttaatggg caaatattgc ccttaaattc tcttttactt
      301 ttgatttaca gagtaaagcg ttgggataat ctatcttcca agcagattat tgtatttggg
      361 atcaagatca ctgatagata cataacttgt gtgtatcttt ccgccctcaa attattacgg
      421 cggtaaatga ttaagccatc gccgatagac agatttcatt tttacggtca ggcaccttcc
      481 cgggctgaac tggctaaaag ctgaattatt tgcattcctc caggagaaat agatggaaac
      541 gactcaaacc agcacgattg cgtcgaaaga ctctcgtagt gcctggcgca agacagacac
      601 catgtggatg ctgggccttt acggcacggc aatcggcgcg ggcgtgctgt tcctgccaat
      661 caacgccggt gttggcggta tgatcccgct gatcatcatg gctatccttg cgttcccgat
      721 gacgtttttt gctcaccgcg gcctgactcg cttcgtactg tctggtaaaa acccgggcga
      781 agacatcacc gaggttgtag aagaacactt tggtattggc gcaggtaaac tgattaccct
      841 gctctacttc ttcgctatct acccgatcct gctggtttat agcgtggcaa tcaccaatac
      901 cgttgaaagc ttcatgtctc accagctggg tatgacgcca ccgccgcgtg cgattctgtc
      961 gctgatcctg atcgtgggta tgatgaccat cgttcgcttt ggtgagcaga tgatcgttaa
     1021 agcgatgagt attctggtat tcccgtttgt tggcgtactg atgctgctgg ctctgtacct
     1081 gatcccgcag tggaacggcg cagcactgga aacgctgtct ctggacactg catctgcaac
     1141 cggaaacggt ctgtggatga ccctgtggct ggcaattccg gtaatggtgt tctcgttcaa
     1201 ccactctccg atcatctctt ctttcgccgt tgcgaagcgt gaagagtacg gcgatatggc
     1261 agaacagaaa tgctcgaaga tcctggcatt cgcacacatc atgatggtgc tgaccgtaat
     1321 gttcttcgtc ttcagctgcg tactgagcct gactccggca gacctggctg cggctaaaga
     1381 gcagaacatc tcgattctgt cttacctggc taaccacttt aacgcaccgg ttatcgcgtg
     1441 gatggctccg attatcgcga ttatcgctat caccaaatcc ttcctcggcc actacctggg
     1501 cgcacgtgaa ggcttcaacg gtatggtgat taaatctctg cgtggtaaag gtaagtctat
     1561 cgaaatcaac aagctgaacc gtatcactgc gctgttcatg ctggtaacga cctggattgt
     1621 tgccaccctg aacccgagca tcctgggtat gattgaaacc ctgggcggcc caatcatcgc
     1681 gatgatcctg ttcctgatgc cgatgtacgc aattcagaaa gtaccggcaa tgcgtaagta
     1741 cagcggtcac atcagcaacg tattcgttgt cgtgatgggt ctgattgcaa tctccgcaat
     1801 cttctactct ctgttcagct aagtcctttc gcgccgcttt cgggcggcgc ttcctccgtt
     1861 ttaacgcgat gtatttccta tgattagcgt attcgatatt ttcaaaatcg gcattggccc
     1921 ttccagttct cataccgttg gaccaatgaa agcgggtaaa caatttaccg acgatctgat
     1981 tgcccgtaac ctgcttaaag acgtgacccg cgtggtggtt gacgtgtacg gctcgctctc
     2041 tctgactggt aaaggccacc acacagatat cgccattatt atgggcctgg cgggtaacct
     2101 gccggatacc gtggatatcg attccatccc cggttttatt caggatgtga atactcatgg
     2161 tcgcctgatg ctggcaaacg gtcagcatga agtggagttc ccggttgatc agtgcatgaa
     2221 cttccatgca gacaaccttt ctctgcatga aaacggtatg cgcattaccg cgctggcggg
     2281 cgataaagtc gtttacagcc agacttacta ctctattggc ggtggcttta tcgttgatga
     2341 agagcatttt ggccagcagg atagcgcacc ggttgaagtt ccttatccgt acagttcagc
     2401 agccgatctg caaaaacatt gtcaggaaac cgggctgtca ctctctggcc tgatgatgaa
     2461 aaacgagctg gcgctgcaca gtaaagaaga gctggaacag cacctggcga acgtctggga
     2521 agtcatgcgt ggcggtattg agcgcggtat ttccaccgaa ggcgtgttgc ctggcaaact
     2581 gcgcgttcca cgccgtgctg cggcactacg ccggatgctg gtcagccagg ataaaaccac
     2641 cactgacccg atggcggttg ttgactggat caacatgttt gcactggcag tgaacgaaga
     2701 gaacgctgct ggcggtcgcg tggtgactgc gccgactaac ggtgcgtgcg ggattatccc
     2761 ggctgtgctg gcgtactacg ataagtttat ccgcgaagtg aacgctaact cactggctcg
     2821 ttacctgctg gtagccagcg ccattggttc tctttataag atgaacgcgt cgatttctgg
     2881 tgccgaagtg ggttgccagg gtgaagttgg cgtggcgtgc tctatggcgg cagctggtct
     2941 ggcagaactg ttaggcgcaa gcccggcgca ggtttgcatc gcagcggaaa tcgcgatgga
     3001 gcacaacctc ggtctgactt gtgacccggt cgcggggcag gttcaggtac cgtgcattga
     3061 gcgtaacgcc attgcagcag tgaaagcggt gaacgccgcg cgtatggcac tgcgccgtac
     3121 cagcgaaccg cgcgtctgcc tcgataaagt tatcgaaacc atgtacgaaa caggtaaaga
     3181 catgaacgcc aagtaccgcg aaacctctcg cggcggcctg gcgatgaaga tcgttgcctg
     3241 cgattaatct ctcccagagg cctcgttttg cgaagcctct tcctgttttc tcaaccagcc
     3301 atagcctgtt ccggcatcga atgttaccct tatcgcctga tctttaaggg ggttatcgtg
     3361 gctgttcatt tgcttattgt cgatgcactg aatcttatcc gtcgcattca tgccgttcag
     3421 ggttcgccct gtgtcgaaac ctgccagcat gcgctcgatc agctcattat gcacagccag
     3481 ccaacccacg cggtcgccgt ttttgatgat gaaaaccgca gtagcggctg gcgtcatcag
     3541 cgtttaccag attacaaagc ggatcgaccg ccaatgccgg aagagttgca cgacgagatg
     3601 cctgcattac gcgccgcctt tgagcaacgc ggcgtcccgt gctggtcagc cagcggcaac
     3661 gaagccgatg acttagccgc cacactggcg gtcaaagtga cacaggccgg gcatcaggca
     3721 acgattgttt cgacagataa aggctactgt cagttacttt caccgacatt acgtattcgt
     3781 gattacttcc agaaacgttg gctggatgcg ccatttatcg ataaagaatt tggcgttcaa
     3841 ccgcagcagt tgcccgatta ctggggactt gcggggatca gcagttcaaa ggtaccgggt
     3901 gttgcgggaa tcggaccaaa aagcgccacg cagctgctgg tcgagtttca gagtctggaa
     3961 gggatatatg agaatctgga tgcggttgcc gaaaagtggc gcaaaaaatt agaaacccat
     4021 aaagagatgg cgtttctgtg ccgcgatatt gcccgcttac aaaccgattt gcatatcgac
     4081 ggcaatttac agcaattgcg gttggtacgg taacggcgag ccggatacgc cgcaaatcgt
     4141 atccggcatt atcacatcag cgcatttacc aggcggtatg gtaaagctct acaatatcct
     4201 caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc atccagtgcc gcctgagcca
     4261 gtgccgaaat gtcttccttg cgtacaccaa catcacgcaa atgtggcgga ataccgacat
     4321 cacggttgag ggcaaacacc gcttcaacag cggcattacg cgccccttcc aggctcatac
     4381 cttccacttt cacgcccata acgcgcgcga tatcgcggta cttctcaccg gtaaagtcag
     4441 cgttatagcg catgacatgc ggtaacagga tggcgttcgc aacaccgtgt ggagtgttat
     4501 aaaacgcgcc cagtggatgc gccataccat gcaccaaccc taacccaaca ttcgagaagc
     4561 ccatacccgc aacatactgc ccgagcgcca tttcttctcc ggcatcctta tcaccagcaa
     4621 ccgatcctcg cagcgcccca gcaatgattt caatcgcttt aatgtgcagt gcatcggtta
     4681 gtgcccacgc gccacgggta atatacccct caatagcatg agtgagcgca tcaacacccg
     4741 tcgcggcttt cagcgccgga ggcataccat ccatcatgtc agcatcaata aacgccacct
     4801 gcgggatatc atgcggatca acgcaaacaa acttgcgccg tttttcttcg tcagtgatca
     4861 cgtagttaat ggtcacttct gccgcagtac ctgctgtggt ggggattgcc agaatcggta
     4921 cactgggttt attggtcggg gaaagccctt ccaggctacg cacatcggca aactccgggt
     4981 tgttgctgat aatgccaatt gctttacaag tatcctgtgg agaaccacca ccaatagcga
     5041 tcaggtaatc cgcgccgcta ttctggaata caccgagccc ttctttgacg acagtaattg
     5101 ttgggttggg cactacgccg tcgtaaatcg cccatgccag ccctgcagca tccatcttat
     5161 cggtcacttt cgccaccacg ccgcattgca ccagcgtttt atcggtgacg atcagcgcct
     5221 tctgataacc acggcgtttc acctcatcgg ttaaagcccc aacagcaccc cgaccaaacc
     5281 atgccgtttc gttcagaatc attctgttag ccatcatcct tctccttgtt gctttacgaa
     5341 attactcttc aatccgtaac ccataggttt tgaatttctc cagcactacg gcaatctctt
     5401 catcgctcag cactggcacc gggtccgtaa tcgccagggt cgtcaggtaa agttgcgcca
     5461 gcacttcaac ttcatgcgcc agccataacg ctttttccag attcacctca caagcgataa
     5521 gcccatgatg ttgtaacaaa gttgccttac gatttttgag agccagcgca acatgttcag
     5581 aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg aatagaatta ccgccagccg
     5641 ccgcaatcat gtagtgaata gcggggatcg atcggttaag aatggaaact gccgtgcaat
     5701 gaacggcatg attgtgaaca accgcgttgg catccggtct gctttgatag gctgccatat
     5761 ggaaacgcca ttcgcttgag gggagctttc cttcctcatg tttaccgttg ccatcaataa
     5821 agacaatatg cgactccgtc agtttttcat atggaatgcc tgtaggcgta atcagcatcc
     5881 catcctgata acgtacactg acgttccccg ctgtcccctg gttcagtccc aggcgggtca
     5941 tttccaggca agtgtcaata atctgacgag caagtttatt tcgttccatt agctacctct
     6001 ctctgattca aaacagggca ataatgttgt tcctttcaca ctattgaatt agccgtttaa
     6061 ttacccacca tcttcttcct gattaacaag aaagaaattc acaagcttat attttgtgac
     6121 ctagctcaac taatcacagt aaataactgc aagttctctt tttataaccc cgttaaaaat
     6181 gaccgctctt aaaaatattt atcaaaacgg tcatttttcc atttcccctc gccagaaatg
     6241 cccgttttcg gcacaaacaa ttaatacggt catctggttt gtgtttttta tgatttattt
     6301 tctgaaacgg gcatgaaatt tcgattatta aagtgatggt agtcacataa agtcaccttc
     6361 cagctaataa gtgtgaccgc cgtcatatta cagagcgttt tttatttgaa aatgaatcca
     6421 tgagttcatt tcagacaggc aaatattcac cgatatgaag cccgaactcg ctggttttgc
     6481 acttttgaaa acataaccga ttacgtgctt aagcttctga acctaagagg atgctatggg
     6541 aaacacatca atacaaacgc agagttaccg tgcggtagat aaagatgcag ggcaaagcag
     6601 aagttacatt attccattcg cgctgctgtg ctcactgttt tttctttggg cggtagccaa
     6661 taaccttaac gacattttat tacctcaatt ccagcaggct tttacgctga caaatttcca
     6721 ggctggcctg atccaatcgg ccttttactt tggttatttc attatcccaa tccctgctgg
     6781 gatattgatg aaaaaactca gttataaagc agggattatt accggattat ttttatatgc
     6841 cttgggcgct gcattattct ggcccgctgc agaaataatg aactacacct tatttttagt
     6901 tggcctattt attattgcag ccggattagg ttgtctggaa actgccgcaa acccttttgt
     6961 tacggtatta gggccggaaa gcagtagtca cttccgctta aatcttgcgc aaacatttaa
     7021 ctcgtttggc gcaattatcg cggttgtctt tgggcaaagt cttattttgt ctaacgtgcc
     7081 acatcaatcg caagacgttc tcgataaaat gtctccagag caattgagtg cgtataaaca
     7141 cagcctggta ttatcggtac agacacctta tatgatcatc gtggctatcg tgttactggt
     7201 cgccctgctg atcatgctga cgaaattccc ggcattgcag agtgataatc acagtgacgc
     7261 caaacaagga tcgttctccg catcgctttc tcgtctggcg cgtattcgtc actggcgctg
     7321 ggcggtatta gcgcaattct gctatgtcgg cgcacaaacg gcctgctgga gctatttgat
     7381 tcgctacgct gtagaagaaa ttctaggtat gactgcaggc tttgccgcta actatttaac
     7441 cggaaccatg gtgtgcttct ttattggtcg tttcaccggt acctggctca tcagtcgctt
     7501 cgcaccacac aaagtcctgg ccgcctacgc attaatcgct atggcactgt gcctgatctc
     7561 agccttcgct ggcggtcatg tgggcttaat agccctgact ttatgcagcg cctttatgtc
     7621 gattcagtac ccaacaatct tctcgctggg cattaagaat ctcggccagg acaccaaata
     7681 cggttcgtcc ttcatcgtta tgaccattat tggcggcggt attgtcactc cggtcatggg
     7741 ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa ctgatccccg cactctgctt
     7801 cgcggtcatc tttatctttg cccgtttccg ttctcaaacg gcaactaact gaacatattt
     7861 tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct taccgaaaat tggtatccgc
     7921 ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc ttgaagaaca aacaatgaat
     7981 atggcgaaag ctacggccgc actgctgacc gagaaactgc gccatgcctg cggagctgcc
     8041 gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg ctgaagccgc tgcttgcgaa
     8101 gaaaaattca gcagccagaa tgtaggcctc accattacgg taacgccttg ctggtgctat
     8161 ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag ccatttgggg ctttaacggc
     8221 actgaacgcc ccggcgctgt ttacctggct gcggctctgg cagctcacag ccagaaaggc
     8281 atcccggcat tctccattta cggtcatgac gttcaggatg ccgatgacac atcgattcct
     8341 gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg gtttggccgt cgccagcatg
     8401 aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg gtatcgccgg ttctattgtt
     8461 gatcacaact tctttgaatc ctggctggga atgaaagtcc aggcggtgga tatgaccgaa
     8521 ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg aactggaaat ggcactggcc
     8581 tgggctgata aaaacttccg ctatggcgaa gatgaaaata acaaacagta tcaacgcaat
     8641 gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga tggcgatgtg tatccgcgac
     8701 atgatgcaag gcaacagcaa actggccgat attggtcgcg tggaagaatc acttggctac
     8761 aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga ccgatcaata tcccaatggt
     8821 gacaccgccg aagcgatcct caacagttca tttgactgga atggcgtgcg cgaacccttt
     8881 gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa tgctaatggg tcaccagctc
     8941 accggcaccg ctcaggtatt tgccgatgtg cgtacctact ggtcaccaga agcaattgag
     9001 cgtgtaacgg ggcataaact ggacggactg gcagaacacg gcatcatcca tttgatcaac
     9061 tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg acagcgaagg taacccgacg
     9121 atgaagccgc actgggaaat ctctcagcaa gaggctgacg cttgcctcgc cgctaccgaa
     9181 tggtgcccgg cgatccacga atacttccgt ggcggcggtt actcttcccg cttccttacc
     9241 gaaggcggcg tcccgttcac catgactcgt gtcaacatca tcaaaggcct gggaccggta
     9301 ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg atgtgcatga catcctcaac
     9361 aaacgcacca actcaacctg gccaaccacc tggtttgcac cgcgcctcac tggtaaaggg
     9421 ccgtttacgg atgtgtattc ggtaatggcg aactggggtg ccaaccacgg agttctgacc
     9481 atcggccacg ttggcgcaga ctttatcacc cttgcctcca tgctgcgtat cccggtatgt
     9541 atgcacaacg ttgaagagac caaagtgtat cgtccttctg cctgggctgc gcacggcatg
     9601 gatattgaag gccaggatta ccgcgcttgc cagaactacg gtccgttgta caagcgttaa
     9661 tacattacac cctgatgtga tgcccggtcg ctccggctac cgggcctgaa caagcaagag
     9721 tggttagccg gataaacaat gttatccggc tatattgcag gagcgattat gaaacaagaa
     9781 gttatcctgg tactcgactg tggcgcgacc aatgtcaggg ccatcgcggt taatcggcag
     9841 ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg atatcgcgat ggaaaacaac
     9901 acctggcacc agtggtcttt agactgccat tttgcaacgc tttgctgatt gctgtcggca
     9961 aatcaatagt gaactgactg aatgccacat ccgcggtatc gccgtcacca cctttagtgt
    10021 ggatggcgct ctggtagata agcaaggcaa tctgctctat ccgatcatta gctggaaatg
    10081 tccgcgaaca gcagcggtta tggacaatat tgaacggtta atctccgcac agcggttgca
    10141 ggctatttct ggcgtcggag cctttagttt caatacgtta tataagttgg tgtggttgaa
    10201 agaaaatcat ccacaactgc tggaacgcgc gcacgcctgg ctcttcattt cgtcgctgat
    10261 taaccaccgt ttaaccagcg aattcactac tgatatcacg atggccggaa ccagccagat
    10321 gctggatatc cagcaacgcg atttcagtcc gcaaatttta caagccaccg gtattccacg
    10381 ccgactcttc cctcgtctgg tggaagcggg tgaacagatt ggtacgctac agaacaacgc
    10441 cgcagcaatg ctcggcttac ccgttggcat accggtgatt tccgcaggtc acgataccca
    10501 gttcgccctt tttggcgctg gtgccgaaca aaatgaaccc gtgctctcct ccggtacatg
    10561 ggaaatttta atggttcgca gcgcctaggt tgatacttcg ctgttaagtc agtacgccgg
    10621 ttccacctgc gaactagata gccaggcatg gttgtataac ccaggtatgc aatggctggc
    10681 atccggcgtg ctggaatggg tgagaaaact gttctggacg gctgaaacac cctggcaaat
    10741 gttgattgaa gaagctcgtc tgatcgcacc tggcgcggat ggtgtgaaaa tgcagtgtga
    10801 tttattgtcg tgtcagaacg ctggctggca aggagtgacg cttaatacca cgcgggggca
    10861 tttctatcgc gcggcgctgg aagggttaac tgcgcaatta cagcgcaatc tacagatgct
    10921 ggaaaaaatc gggcacttta aagcctatga attattgtta gtcggtggag gaagtcgcaa
    10981 cacattgtgg aatcagatta aagccaatat gcttgatatt ccggtaaaag ttctcgacga
    11041 tgccgaaacg accgtcgcag gagctgcgct gttcggttgg tatggcgtag gggaatttaa
    11101 cagcccggaa gaagcccgtg cacagattca ttatcagtac cgttatttct acccgcaaac
    11161 tgaacctgaa tttatagagg aagtgtgaaa tgctgaaaac aatttcgccg ttaatttctc
    11221 ccgaactatt gaaagtgctg gcagagatgg gacatggaga tgaaattatt ttttccgatg
    11281 ctcactttcc cgcccattcg atggaaccgc aggtgatccg cgctgatggc ctgttggtga
    11341 gcgacttgct ccaggcgatt atcccgttat ttgagctgga cagttatgcc ccgccgctgg
    11401 tgatgatggc ggcggtagag ggtgacactc tcgatcctga agtagaacga cgttaccgta
    11461 atgcgctttc actacaagcc ccgtgtcctg acatcatccg catcaatcgt tttgcgtttt
    11521 atgaacgggc gcaaaaagcc tttgcgatcg ttatcacagg cgaacgagcg aagtacggga
    11581 atattctttt aaaaaaaggg gtaacaccgt aatctcatac cggtacgccc gcatgacgcg
    11641 ggcggttatc gaatgatggg gtgaaaaata tgaaagcggc acgccagcaa gcgatagtcg
    11701 acctgctgct gaaccatacc agcctgacca cggaagctct ctctgaacag ctaaaggtca
    11761 gtaaagaaac cattcgtcgc gatctcaatg aattacagac gcagggtaaa attctgcgca
    11821 atcatggacg cgctaaatat atccaccgtc aaaatcaaga cagtggcgat ccctttcaca
    11881 tcaggctgaa aagccattat gcgcataaag cagatatcgc gcgcgaggcg ctcgcgtgga
    11941 ttgaagaagg gatggtgata gccttagacg ccagttcaac ttgctggtat ctggcacgcc
    12001 agttgcctga catcaacatt caggtcttca ccaatagcca tccgatttgc catgaactcg
    12061 gtaaacgcga acgcattcaa ctgatcagtt ccggcggcac acttgagcgc aaatatggct
    12121 gttacgtcaa tccctcgctg atttcccaac ttaaatcgct ggaaatcgat ctgtttattt
    12181 tttcttgtga agggatcgat agcagcggcg cactgtggga ctccaatgcg atcaacgctg
    12241 attacaaatc gatgctatta aaacgtgccg cgcaatcgtt gttattgatt gataaaagta
    12301 aatttaatcg ttcaggggaa gcccgcatcg ggcatctgga tgaggtaacg cacattattt
    12361 ctgatgagcg ccaggttgca acttctttgg taacagcctg acggaaaggg tagcaggccg
    12421 gagacgacgc cccggccttg cctgttatcg ctcgtcgcga cgaccaccca ccgcagccca
    12481 gatgcggcgg acgtgcaccg tcacttcttc gcgatcgtga tacaactgcc gcgcctgaat
    12541 ctgagcattt atgccatgtt catcaagctg tgcctgaata tacgccagat tgtgtgatac
    12601 ttcttcgtag cgttttttca tcggcagttt gagattgaaa atggtttcac ggcaccagcc
    12661 attaaccagc cactgcgcca tcaatgccgc aactttagcc ggtttttcaa ccatatcgca
    12721 taccatccag gagatattgc tgcgtgtcgg acggaatttg aaaccgtctt cccgcagcca
    12781 cgtcacctgc ccggtatcca tcagactttg cgccatcggg ccgttgtcga cggaataaac
    12841 ccacatgttg cgcttcacca gttggtaggt ccagccgcca gggcaagcgc ctaaatccac
    12901 cgcccacatc ccgttcgcca ggcgttcatc ccactcatcc gcaggaataa acacatgaaa
    12961 tgcctcttcc agtttgagcg tggaacgact cggcgcctct gccggaaatt tcaggcgcgg
    13021 aatgcccata tagaacggcg aattattgtt gctgtatgag taaccggtat agcagcatcc
    13081 tggtgcaata aagaatacat gcacaaccgg acgcttcggc gtttcatagt tcgccagcac
    13141 cccagcatcg cgcaaggcag cgcgtagcgg aacagtgaac ttacggcaga atttcagcaa
    13201 ctctttgctt tcgttggtat cggcaacttc aacacgcagt tcaccgccct tctctactac
    13261 accctgtaac atgccgacaa tgggggtaat acgatcttct ggcggcaaat gctgcaggag
    13321 ttcccccacc acaaaccact ggcgggcaaa aattaatgaa ctgaacggca gctcacggat
    13381 taacttatcg ccatcatcag gttgataaca ttcataaatg acataacccg cattctcttt
    13441 cacgcgggca aaaccgaaaa tttcccgctg gccggcttta tcggtaattt ctgcggcgca
    13501 ctctttttca aagcccggac ggcacagcaa tacaacctta ttcatgactt acgcccttgc
    13561 gctttaaacg gatagcacca actaacatta acgcccagcc cgcaaggaag ctcacgccgc
    13621 caaccggtgt gacaaacgcc cacaaacgca gatgggacag cgccaggcaa taaaggctgc
    13681 cgctgaacag caccgtgcct aacgcgagga aaacgctact ccagtaaaac cagatactga
    13741 tgcgacgctg cattgccacc gccagaccta agatcgccag cgtatgaaac gcctggtatt
    13801 cgagaccggt ttggatccag cccatctcaa cggcccccat ggttttactt aacacatgcg
    13861 caccaaaagc gcccagtgcc acaaaaatga agccgctaat ggcggcgaaa atcagcataa
    13921 aacggctggt catggtcgta ccctacgtaa attattgttc ataacgaaag cggaattttt
    13981 cttgttcagc agcggctttc gccaggatcc attggcgaaa ggcggctatt ttacccagtt
    14041 ctgcctggct gtcatgacaa accagataaa aagcattttt actgaccaga acatcattaa
    14101 acgggcaaac aagacgtccg gcctcgattt cagattgcgc catcacgtta tttgccagcg
    14161 ccactccctg cccgtgaata gccgcttgca gcaccatggc gctatgacta aaaattggcc
    14221 cttgctgaac gttgatatga ttaaacccca actgtcgggt atatgtctgc cagtcacggc
    14281 gtgacgcatc atgtaataac gtatgtttag ccagatcttc cggggtcttc aagggttttt
    14341 cgcctgtcag cagtagcggc gaacacaccg gcaataaata ttcggcgtac agtttttcca
    14401 cccgtagccc cggccagttg ccccgaccat aaaatatcgc cacatcaaca tcatccgcca
    14461 gcttatcttc ctgacgatca accgcctgga ttcgaacgtc aattcccgga taagctgaat
    14521 taaagctgga aagtcgcgga accaaccaat gaatggcgaa actggggagt aaactgaccg
    14581 tcaacgcccc cttggcgcta cgggcctgga gtttacgcgt cgcttcggtt aattgcgaaa
    14641 atatctcttt gatatcgagg aaatagcttt gcccttcctc ggtcagcagg agtgaacgat
    14701 tacggcggcg gaacagtttt agccccaaaa aatcctcaag agacttgatt tgatgactta
    14761 ctgcggcctg ggtcacaaaa agctcttctg ctgcgcgagt aaaacttaaa tggcgtgctg
    14821 cggcatcaaa aactcgtaag gcatttagcg gtggtaatcg tttagacatg gcttttaaac
    14881 tttgatgtta aatgaattta acaattagat cacactgtgt aacctattag tttttttaat
    14941 ctgagccatt ataaattgtc cgttgagctt ctaccagcaa atacctatag tggcggcact
    15001 tcctgagccg gaacgaaaag ttttatcgga atgcgtattc tggtgaactt ttggcttacg
    15061 gttgtgatgt tgtgttgttg tgtttgcaat tggtctgcga ttcagaccat ggtagcaaag
    15121 ctaccttttt tcacttcctg tacatttacc ctgtctgtcc atagtgatta atgtagcacc
    15181 gcctaattgc ggtgcttttt tttaccttgc gatcgcgaat tactgttcca gttcgaccat
    15241 ctctttcacg tcagtacggt tgatctgctg tttgttgcca ttagcgtctt tatacgaaat
    15301 cataccgata tcgttatcag tctgtggttt gccgtcagag acgatggtac gtccgtcatt
    15361 ggtgtgcatc acatagttcg aaccggaaca ggcgctcagg gcaaaagtca gcatacaggc
    15421 agaaataatt gcggcagtct ttttcatcat cttctcctta aaacgattaa taaatcttaa
    15481 tagtattgat taacaggcta aaattaacgc ctaacactat tcagcatatg ttacttacgc
    15541 ggcttcgcca ggatatccag ataattctga tggttagcac tctccttgta tcaaagtgaa
    15601 ttttgcgtca cgatcggtgc atcaagccga ggagtaccat gaacgttttt aatcccgcgc
    15661 agtttcgcgc ccagtttccc gcactacagg atgcgggcgt ctatctcgac agcgccgcga
    15721 ccgcgcttaa acctgaagcc gtggttgaag ccactcgaca gttttacagc ctgagcgccg
    15781 gaaacgtcca tcgcagccag tttgccgaag cccaacgcct gaccgcgcgt tatgaagctg
    15841 cacgagagaa agtggcgcaa ttactgaatg caccggatga taaaactatc gtctggacgc
    15901 gcggcaccac tgaatccatc aacatggtgg cacaatgcta tgcgcgtccg cgtctgcaac
    15961 cgggcgatga gattattgtc agcgtggcag aacaccacgc taacctcgtc ccctggctga
    16021 tggtcgccca acaaactaga gccaaagtgg tgaaattgcc gcttaatgcg cagcgactgc
    16081 cggatgtcga tttgttgcca gagctgatta ctccccgtag tcggattctg gcgttgggtc
    16141 agatgtcgaa cgtgactggc ggttgcccgg atctggcgcg agcgattacc tttgctcatt
    16201 cagccgggat ggtggtgatg gttgatggtg ctcaggggac ggtgcatttc cccgcggatg
    16261 ttcagcaact ggatatcgat ttctatgctt tctctggtca caaactgtat ggcccgacgg
    16321 gtatcggcgt gctgtatggc aaatcagaac tgctggaagc gatgtcgccc tggctgggtg
    16381 gcggcaaaat gattcacgaa gtgagttttg acggtttcac cactcaatct gcgccgtgga
    16441 aactggaagc aggaacgcca aatgtcgctg gcgtcatagg attaagcgcg gcgctggaat
    16501 ggctggcaga ttacgatatc aaccaggccg aaagctggag ccgtagctta gcaacgcttg
    16561 cagaagaagc gctggcgaaa cgtccaggct ttcgttcatt ccgctgccag gattccagcc
    16621 tgctggcctt tgattttgct ggcgttcatc acagcgatat ggtgacgcta ctggcggagt
    16681 acggtattgc cttgcgggcc gggcaacatt gcgctcagcc gctactggca gaattaggcg
    16741 taaccggcac actgcgcgcc tcatttgcgc catataatac aaagagtgat gtggatgcgc
    16801 tggtgaatgc cgttgaccgc gcgctggaat tattggtgga ttaatgacaa acccgcaatt
    16861 cgccggacat ccgttcggca caaccgtaac cgcagaaacg ttacgcaata ccttcgcacc
    16921 gttgtcgcaa tgggaagata aatatcgcca gttgatcatg ctggggaaac agcttccggc
    16981 tttgccagac gagttaaaag cgcaggctaa agagattgcc ggatgcgaaa accgcgtctg
    17041 gctgggatat accgtggctg aaaacggcaa aatgcacttc tttggcaaca gcgaagggcg
    17101 cattgtgcgc ggcctgttgg cggtgttgtt gactgccgtt gaggggaaaa ccgccgccga
    17161 attacaggca cagtcgccgc tggcattgtt tgatgagctg ggattacgtg cgcagcttag
    17221 cgcctcacgc agccaggggt taaatgcgtt aagcgaggcg attatcgctg cgacgaagca
    17281 ggtttaagca cagaaccggg tcggataaga cgttcgcgtc tcatccgacc tgattgttta
    17341 accctgacgc gccgctttcg ccatcatctt cttcagcgca tgagaaaccg caacaaaccc
    17401 aaaggtggcg gtcaccatcg ttgccgcgcc aaatccagat gcacaatcca tccgcttcgg
    17461 cccttctgcc gtggctttca tcgcacacac cgtaccgtct gactgcgggt acaccagcgc
    17521 ttcagtagaa aacacgcaat ccacgccgag cttaccttta ctgtttttca ctacgccaaa
    17581 atcgcttttc aggcgctcgc gcaacttcgc cgccagcgga tcctgaatcg ttttcgccag
    17641 atcggtaacc tgaatctgcg tcgggtcaat ctgcccaccc gcgccaccgg tcgtgaccag
    17701 cgggatttta ttgcgccgac aataagcaat cagcgccgct ttgggccgta cactatcaat
    17761 ggcatcaatc acgtacgaat aaccggcact catatattgc gctacgttat ccggcgtcac
    17821 gaaatcatcc accaccgtta cacggcactc cgggttaatc tggcgaatgc gctccgccat
    17881 gacttccgct tttgccagcc cgacgttatc gcgcagagcg tgaatttgcc gattggtatt
    17941 ggtgacgcac acatcatcca tatcgataag cgtgattgcg ccaatccccg tgcgcgccag
    18001 cgcttccgcc gcccaggaac cgacaccacc gataccaacc acacaaatat gcgcgtcagc
    18061 aaacagttgc aacgcttttt caccatacag acgcgctgtg ccaccaaaac gctgacgcca
    18121 tgcatcacta attaccacag acatagcacc tcagaattaa aaagggcgag gttatccctc
    18181 accctaaacg cataaatgcc tgatgcgcta cgcttatcag gcctacataa ctcctgcaat
    18241 attttggatt tgcatgattt gtaggccgga taaggcgttc acgccgcatc cggtatgaac
    18301 aacgcgcact tagtcaataa tcaggatgtg aggttacccc tcccctgtca tatccgtaaa
    18361 aacggcatac agaatatcac atcagccgct aaagacgtta cctgcgcccg gagccgtttt
    18421 cagcacccaa acccgtccat agtggttgta ccaacctgcg cggtgtccgg cttccggccc
    18481 gatcccttga tagatatcga agtgttggcc tttgattgcg cccccgacat ccaacgccac
    18541 catcagacgc aattcgtact gaccgttaaa tttgccgtta ttatccagca gcggcacttc
    18601 tgccagcaag gtagtacctg gcggaataat tgaacgatcg gaggcaacag aagcacggcc
    18661 aaccagcggc accgcacttg cccctttcac cggtgcaaaa gattgcggtt taaagaagac
    18721 gaaagacggg ttctgttcca gcagctcgcg aacctcggct tcactgtgtg tttcgcccca
    18781 atgacgaatc gcctgcatcg acatatcttc ttttttcact tcgccacggt cgatcagcac
    18841 cttaccaatg ctgcgatagg catgaccgtt tttccctgca tagctgaaaa agttaagcgg
    18901 actgccatca ccaaagtcga tatacccact accctgcaca tccataatga agttatccat
    18961 cagagagttg ctgtaagcga ggatgtattt atcgcccaat gccccggcgt agatctccgc
    19021 acgagacggc agacgaccac gttttggcgg catacggtaa ataggatact ggaactcgcc
    19081 ctggcgggta tggcgcgcct gaattaccgg cgtgtaatag cctgtaaact gcacgttacc
    19141 atagttgtcg gcaccttcca tctgccaggc atcaatgccg aactggcgca tattgcgggt
    19201 atcaccgcct gcgcgcagcc actcttgcac cgcgttataa acattactct ggttgccata
    19261 cagacgcggt gacgaattac ggatatggtt aatttgctcg acaaaatcac cggcgttaat
    19321 cggcgcgcca acggcatctg gctggttcac cagagagaaa ggctgggtaa atttcccgtc
    19381 tttatattgc tgtccgcgat cggttggttt ggaagagcag gcggcaagca ttgccacaac
    19441 cgtgcccata agaaggtact ttacccaacg tcctttcatt gttctcttct tcaggttaaa
    19501 aaataaggcg caacgaagat aacaaaccgc cgcgtttaat gaaatgagca gcctctccct
    19561 gacgcaaatt ttgcacaaaa aataggcttt agtggtttgt ttttgttcaa aatcatgcta
    19621 aatccgtgat cggggtaaaa aaaaggttgc atgaaaacgc gagcggagta tagtgcgcat
    19681 ccacggacgc ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg
    19741 gttcaaatcc ggcccccgca accaatcaaa tttgatgaag taaaagcagt acggtgacgc
    19801 ggggtggagc agcctggtag ctcgtcgggc tcataacccg aaggtcgtcg gttcaaatcc
    19861 ggcccccgca accaattatt gaacacccta acgggtgttt ttttgtttct gatctcccat
    19921 aaaaaaagcg ccattcagcg cctttttatc atccccttct cgccagcgtt gccccatcgg
    19981 caaaatacgc tttaatcccc gcaagaatag actccgcaac ttcctgctgg aaagtcgccg
    20041 ttttcagttt acgctcttcc tcaacgttac tgataaacgc cgtttcgacc agaatggagg
    20101 gaatatctgg tgcctttagt acggcaaacc cggcctgttc aacttgattt ttatgcagct
    20161 tgttgatttt tccgagctta ttcagtaccg ctttaccaaa cttaaggctg tcagcaatag
    20221 tcagcgactg caccatatcg aacatggtgt ggtcgacata gcggtcaccg cttttgctca
    20281 cgccaccaat caagtccgag gcgttctggg tttgtgccag atattttgcc gcagtactgg
    20341 ttgcgccttt ggttgagagc gcaaacaccg aggaaccgct cggctgacga ctggtaaagg
    20401 cgtcggcatg gatagagaca aacaggtcag cacgctgttt ctgggctttt gctacgcgca
    20461 cttgcaacgg aatgaagatg tcttcattgc gcgtcatgta caccttcata ttgccctctt
    20521 cctcgatcag agagcgcaaa cggcgagcta tttgcaatac cacatctttt tcgcgcgttt
    20581 tgtatttccc caccgcaccg gagtcttcgc caccgtggcc agggtcaagc ataatgacaa
    20641 tcggacgatc gcgccctgct ttacccggtt gtggaccact ttgcgctggc ggcacctgct
    20701 tttcgaggtc gcctttgttg taatcctcca gcagcgccag cagcgggtcc tgcatatcct
    20761 gtgcattggc cggatagagg tccatcacca gacgctcttt aaacccggcg accggcgcaa
    20821 gggcaaacag ctgcggtttt acgttttgct ttaattcaaa aaccatgcgt acggtttgcg
    20881 ggtcaaattg cccgacgcgc gccgacttga tgaacgggtc gtctgcacga atttgtgccg
    20941 ccatcccctt gagtaccgag ttcaggttta catcttcgat atccaccacc acgcgttcag
    21001 ggttactcaa cgcgaactgc ttatatttca gctgacgatt tgattctacc gtcacgcggg
    21061 tgtagctgga cgcaggccag acgcgcaccg ccacgacctg gctaaccgca gccaggctga
    21121 cctgacttac gctcaatagc cacatagcgc ccgcgccttg cagtaaacga cgacggctga
    21181 ttgcagtgtt ggatcctgac atgcctctcc cgagcaaaag aaatctaagc tgtgtaacaa
    21241 gtaaacgact aatttgaccg gaaactttag cgaaagacgc ataatctgtc atctaataaa
    21301 cggtaaacat tctttttata ttcacggcat tactgataaa aaagtcgctc tcgcataaaa
    21361 tttacacttg caccctgcga aaaaacagaa taaaaataca ataatttcaa ataatcatgc
    21421 aaaggggtgt gccgtggtaa aggaacgtaa aaccgagctg gtcgagggat tccgccattc
    21481 ggttccctat atcaataccc accggggaaa aacgtttgtc atcatgctcg gcggtgaagc
    21541 cattgagcat gagaatttct ccagtatcgt taatgatatc gggttgttgc acagcctcgg
    21601 catccgtctg gtggtggtct atggcgcacg tccgcagatc gacgcaaatc tggctgcgca
    21661 tcaccacgaa ccgctgtatc acaagaatat acgtgtgacc gacgccaaaa cactggaact
    21721 ggtgaagcag gctgcgggaa cattgcaact ggatattact gctcgcctgt caatgagtct
    21781 caataacacg ccgctgcagg gcgcgcatat caacgtcgtt agtggcaatt ttattattgc
    21841 ccagccgctg ggcgtcgatg acggcgtgga ttactgccat agcgggcgta tccggcggat
    21901 tgatgaagac gcgctccatc gtcaactgga aagcggtgca atagtgctaa tggggccggt
    21961 cgctgtttca gtcactggcg agagctttaa tctgacctcg gaagagattg ccactcaact
    22021 agccatcaaa ctgaaagctg aaaagatgat tggtttttgc tcttcccagg gcgttactaa
    22081 tgacgacggt gatattgtct ccgaactttt ccctaacgaa gcgcaagcac gggtagaagc
    22141 ccaggaagag aaaggcgatt acaactccgg tacggtgcgc tttttgcgtg gcgcagtgaa
    22201 agcctgccgc agcggcgtgc gccgctgtca tttaatcagt tatcaggaag atggcgcgct
    22261 gttgcaagag ttgttctcac gcgacggtat cggtacgcag attgtgatgg aaagcgccga
    22321 gcagattcgt cgcgcaacaa tcaacgatat tggcggtatt ctggagttga ttcgcccgct
    22381 ggagcagcaa ggtattctgg tgcgccgttc tcgcgagcag ctggagatgg aaatcgacaa
    22441 attcaccatt attcagcgcg ataacacgac tattgcctgc gccgcgctct atccgttccc
    22501 ggaagagaag attggggaaa tggcctgtgt ggcagttcac ccggattacc gcagttcatc
    22561 acggggcgag gttctgctgg aacgcattgc cgctcaggcg aagcagagcg gcttaagcaa
    22621 attgtttgtg ctgaccacgc gcagtattca ctggttccag gaacgtggat ttaccccggt
    22681 ggatattgat ttactgcccg agagcaaaaa gcagttgtac aactaccagc gtaaatccaa
    22741 agtgttgatg gcggatttag ggtaagcgat gaaaatcacc ggatgcggca ggcgtaacac
    22801 tcgtgcgtcg catccggcaa ttacgtttat tcccgcgaac taaacaacgc cgccagacca
    22861 ctgcgccgct cagtacgagt ggcgattgcc gcacttaata tacgctcatc ggcatacagc
    22921 gacagacgac gacgcgcgcg ggtcaccgcg gtgtatacca gctctcgcgt tactaccggc
    22981 gtgcgttggc tcggtaaaat caacgccgca tggtcgaact ccgatccctg cgatttatgt
    23041 accgtcatcg cccacgtagt ttcgtgctct ggcaagcgac tcggttgcac agacttaata
    23101 ttgccgtccg gcatcgcaaa ccagacgcgc gtcccctgcc cgcgatccag cgcaataccg
    23161 atatcgccat taaacaaccc aagcgcgctg tcattacggg caatcatcac cggtcggcct
    23221 tcgtaccaac gagagtgcgg atgacgatga attttgcgct tctgttgcat aaactgttca
    23281 attcgctcat tcagtccagc cacgccaaac ggcccttccc gcagggcgca caaaagctgg
    23341 tactcattga acgcctgaat gattaaatcc ggctcggcac gcgcttgcag cagatccaga
    23401 taacgtccgt aacccgcaag agcttcctca agcatcgcaa tataatcttc gccgctctgt
    23461 aaaagtcgtt tttcgatatc agtaaaatcc tgctgaaaaa cggttttcac tgccgtttta
    23521 tcaccacggt tgatcgccgc agctaactga ccaatgccag aatcgctgcc gaaacgatag
    23581 cttttttgca gcaggcagag actgtcgcgc aaagatgccg cttctgtgcc ggttcctgcc
    23641 ggaacgtgag tccccgtcag gcggcttagc tgcctggcac gctcggcggt aaagcccgcg
    23701 ttggcataag cgcagatatc gcccagcaca gccccagcct caaccgaggc cagttgatca
    23761 cgatcgccga gaaagatcac tcgcgcatga tctggcaagg cgtcgatcag tctcgacatc
    23821 ataggcagat cgatcattga cgcttcatct accaccagca catcaagatg cagcgggtta
    23881 ccggcatgat gacgtaaacg ctggctaccc ggctgcgcgc ccagcaatcg gtgcaaagtg
    23941 ctggcatctt ccggaatgcg tttcttttgt tcatcggtca gcggtaactg tcgcaaagcc
    24001 ttgccgagag attcggttaa gcgcgcggca gctttacccg ttggtgcagc cagacggata
    24061 cggcagcgtt cgccgtcggc catttgaatt aacgctgcca gcaacttcgc cacggtggtc
    24121 gttttaccgg tgcctgggcc gccggaaatc accgagatcc gccgcgtcag cgccactgcc
    24181 gctgcaactt tttgccagtt aatttcatcg cttactggaa aaagtttgtc cagggtttgc
    24241 gccagtagag cttcatcaac ctcaatggta tgattcacct cattgaagaa gcgcgctacc
    24301 gtccgctcgt tacaccacat gcgattcaaa taaagacgat cgccacagag gatcatcggc
    24361 gtgggttcat ctcccctgct gaccgcttga gaagccagca agcattcttc ccaattttgt
    24421 agctcaccga tttcactgac acaggtcgcc aacagcggat tcgacgcctc gttattttcc
    24481 agtcgtgaaa gcggcaaaca gacgtgtccc tctccggcat catgacttaa cagtgccgcc
    24541 gcgagggtga cggcaggatg ttcatctccc gccacggtca gggcaaattg cacatccagc
    24601 gggcgtagct gtttgtgctc cacagcttcc agtaattgct tttgcaattt cattacgcct
    24661 cctccagggt cataccggca aacatctcat ccatcagggc aatcaacccg gcgttgggtc
    24721 gggttgtgta aatcccttgt tgcggatgtt ctttatcaac gccacgcagg aacagataga
    24781 taacgccgcc aaagtgaaga tcatagtcgt aatcagcaat gcgatggcgc agataacgat
    24841 gcagcgccag ggtataaagc tgatattgca gatcatagcg gtgtgcctgc atcgctgctg
    24901 ccatagcctg ttgggtgtaa gccgaactgt cttcacccag ccagttggat ttatagtcga
    24961 gcaggtaata acgcccttcg tggcggaata ccaggtcgat aaagcctttt aacatgccgc
    25021 gtacctgcat gaactccagc ggcgggcagc cagcggatag cgggtcaaac tggcgaatta
    25081 gtgcatcaag ctggctggcg atgagcggtt cgctaatcgg caggtaaaac tccatctcca
    25141 cctgtttatc gcggtcggaa agctgactca ggctaacacc cgtttcattg agaggtgcct
    25201 ggaggacagc cgtgatccac tcggtcaaca ccggttccca ctgcgattca aagccgccga
    25261 gttccagttt ttcctgtacc cagttcgggt caaccggctg ggtaaaatcg aggtcttcaa
    25321 acaaactgtg caagaatgtc cccggtgacg caccgcgcgg gaactgatgc ggtgttaacg
    25381 tcggttcttc aacgacgctg accacgcctg cggcatcaac atccagccgt ggcatcagat
    25441 cctgagcgat accgtgacca cgctgctgca agccggagta gctggtgaca cgccagttat
    25501 cgccgggcaa tcgttgtagt gtcctcgcat tcagttctgc agttaaagca tcattaacct
    25561 gccagggctg gttatcacca gtttgtgccg tttgccaggc aatatcatca tcgcataacg
    25621 cttcaataca ggtgcgaagc cctgccgcat cttgcggttc ccctttttgc agcaaacgcc
    25681 cgagcgcact ttggtggacg tcggtgtcac cttttttatc gccacgacgg cgcaccagcg
    25741 gtgcaacgcc gagactgcaa tgccaaaccg aacgcgtcag cgccacgtaa agcaaacgca
    25801 gatcttccgc cagacgttcg acctccgcga ggtcgacgct ttctggcgca gcattaagat
    25861 ccaaaactgc ctcaaacgag tggcgatcgt gataaaacgc ctgatcctgg acgcggaaat
    25921 tggtaataaa tggcaaccag accagcggat attccagccc tttcgattta tggatcgtga
    25981 caatctgcac cagatgttta tcactttcga ggcgcatttg ttggctggag gcattactgt
    26041 ctggctcgag gatatgttgc gataaccagc gtaccagcgc atgttcactt tccagctgcg
    26101 ttccggcttc ttgaagcagt tcgctgatat gcaagatatc ggtaagacgc cgctcaccgc
    26161 ctgccgttgc cagcaagttt tctgcaatgt tacgcgccga catcagcgcc cgcagcatcg
    26221 gcataacgcc acgtttgcgc cagatttgcc gataaccatc gaactcttcg actaccgcat
    26281 cccacgcatg ttcgtcatta ttcagcgttt caatatccag cgcattcagc cccatcattg
    26341 acgttgccag cgcactgcgc agggtgttct cacgttcggg cgtcatcacc gcctgcaaca
    26401 accaaagcat ttcctgcgct tccagagttt caaaaacact gtcgcggttc gaaaggtaaa
    26461 cggaagggat ttccagcaac gttaaggcat cgcgcacctg ggcggcctcc tggcggctgc
    26521 gcaccagcac gctgatgtcc gaagcacgca ccggacgcgc gtcgtcgccg ttcatcagca
    26581 acgcttcgcc ccgctgtccg gcttgtagcc agtcgcggat ttgcgcagca catacctgcg
    26641 ccatggtact ttgatagtcg ccaacgccgc agctttcgcc ttccatcagc cacattttca
    26701 tcgcaggctg cgtttcacct ttaaatacaa aacgtaacgc ctgatttttc ccggctgatt
    26761 tcactggaat aaacggtatt tcgcgaaaca tgaacgtgtc atcagtctgg ctgaaaagct
    26821 tgttcacact gttcaccatt ccaggtgcgg agcgccagtt ggtgtctaaa gtgtagtggg
    26881 catgaacttc actacgcgcc ttcatataag tgaagatatc cgcaccccgg aatgcatata
    26941 tggcctgctt cgggtcgcca atcagcaaca atgcggtttc cggctgatgg tgccagatac
    27001 ggcgaaaaat tcggtactgc tgggggtcgg tatcctgaaa ttcatcgatc attgccaccg
    27061 ggaatcgcgt acggatcgcc gctgccaaca cctcgccgct ttcgctacgc agcgcggaat
    27121 cgagccgact taacatgtca tcaaaaccca attcgccacg gcggcgtttt tcacgcgcta
    27181 ctgtttcgcg gatctcagcc aatgcgcggg tgatcaacag atcgcggatc gacaatggtt
    27241 ctgcaagcag ttgatcgatc gcctcaaaca gtggatgtcg cggggtttcc cccccggcct
    27301 tcgtgcgatc ttctaagaaa cgctgggaga atttttccag cgactccggc aactgataac
    27361 tgtttgtctc ttcttctgcc caggcgctga tcttttcgat ccatttagcc tgattgctac
    27421 ggttaaactt gcgtcgatca ataccagaag attcgatcag cgcatccagt tcacccactg
    27481 cgtcgcgcca ctgctgtttt accgtatcaa tacgcgccac aatttgcgcg tggcgggaag
    27541 ccagcgtttc atcatcgggc ggcggtgctt tgataaccgg cggttcgcct tgcagataac
    27601 gattaatatc gcgcagcaac gcctgcggcc ctttccaggt ttcaaagacg acctgggcta
    27661 tttcacgcgg cagcgggtag cagtggcgac gccagaaatc ggcgcaggcc tggtagcgta
    27721 gcagagactc atcttcaatc agctgctgct caaacagcat gccggattca aaggcattca
    27781 ggttgagcat gcgctggcaa aagccgtgaa tagtaaagac tgccgcttca tccatctgcc
    27841 gttcggctaa caacaaccac tgcgcggctt gcgctttatc gtcgatctct tccagcaggc
    27901 gttcgtacag tggattgtcg gtggtttcac gcagacaggc gatgcgcaac tcgtggatat
    27961 tgctacggat acgaccgcgc aattctgccg tggcagcctc ggtaaaggtg accaccagca
    28021 gttcttcaac gatcagcggg cggggaaagg cggcggaacc gcctagtcca agtaacaggc
    28081 gcaaatagag cgccgcaatc gtaaaggttt tgcctgtgcc ggcagaggct tcaatcaggc
    28141 gctcgccctg taagggcaag cgcaaaggat ctagtgtctc ggcgacatca ctcattcttt
    28201 tcactcatca ggggcattgt ttgctgcaac gcgctgacgt tctcccacac tttccagcct
    28261 tcaggatgca catattcggc tttcccgttc tggctgccgg aaatctgcga cagaatagcc
    28321 atgccttgcg gctcgaccac cgcctgatgg aagaaatcag caagtttttg cggcgtcagc
    28381 agttttatct gggccacgat tttatcacgc gaatcgaagc gcatattgcc gcgatcgaaa
    28441 tctttactta acttcgatgc ttcttcgccg agcgtttgcg gtgcctgcag catctgggta
    28501 attaccgcct gctggatttg cgcaaactca tctggcttca tcgctcgcaa ttttgcctct
    28561 gcggttggga aaaacgcctt gtaacgctcc cacaagaatg aaggctgttt atcattgctt
    28621 tgcaaaagga agcccatgcc ccactgacgc cccacgctca ttggaaacgc aaacacggca
    28681 tagcccaatt gttcttcggt acgcaactga ttgtagaacc acggctgtac gatctgcccc
    28741 aacagagagc tataggctga gctggtgtat tcatcgtagc cagtcggtac aaataccgct
    28801 gccagtgcgg agtcggtgct gttaccggct ttttcaaaga tgacggattg ttttttatcg
    28861 accactacat ctttgtttcg acaccactct gaaccatcag cgcccaactg tttttgcaca
    28921 tcgcgtgcca gcgttgttgc ctgggcctcg gtcatgttgc cgataaccat aaactctggt
    28981 cgagcccctg attttaaggc gtcgcgatag gccagcacct ctttcaacgt aatggagggc
    29041 aaaattttac gccgttcatc tcgcgagaag tacggcactt gcgagagcat ctgcgcgggc
    29101 ataatcgcct gctcaaacgc tttacccttt tctgcggaat ccatcatctg gttataccag
    29161 gacttcgcct gctcaagctg ctcttccgta gcggtatagc taaagtaccc ctcgagcaat
    29221 gcctggaaca gctgcggcag acgctgggtg taaccattag cattaatcat aaggccgttg
    29281 ttagcgttgg tggaaaaact tatgccacca accgacgcct ggttgcttaa ctgatcaagc
    29341 gccagccctg cgagataatc attgagcgca aacatcacct gattgcgggc gctgtccatg
    29401 gctttcggat tacgcaaaat caggctgaca tcagctttgg gctcgctggc aaaataacgg
    29461 cttggcgcat acaccacgcg cagattcgac tcatcaacaa tcagctctgg atggtcgtat
    29521 ttcttctctg acttaatcag cgagaaatca tcaggaatat aagggttaag ctctggcaaa
    29581 gagagcgcaa tgtcggcggc ttttttctgc cagtcggcga aagtttgtgc gctgatttta
    29641 tcgacctgat acggcgcatc gacaaagtaa gccgttttgt tgtgcggctc tttcgggctg
    29701 atataccaga tacgcgcatt ctgcggcgtc atcatcgcca gacgttcctt tactgcttta
    29761 gcatcgtacc gatcggcaat attgactgca tccagcgtat gctcaacagg aacgcgaatc
    29821 atggtatctg ccagccattc gacgtaatcc atatcacggg tgatcgacgg ataacggaag
    29881 tcgatatcca gcacattcgc cagttcatcg aagtattgtt tatcaatgcc tttttcacgt
    29941 aacagattga gatagctaaa aattgccgcc acaacctgat cgcgattagc caggccttta
    30001 tcagttaaag acgcagagat cgctaatacg ccgctgttgc cgttgacgat aggatcggag
    30061 ttggcgctaa tgccctcaac taatccctgc ttttgcagcc agtcagaaag tgtacctggg
    30121 ctgcgattgc caatcagata ggtaatcaat tcatcggttt tactacggaa cttcgctgag
    30181 ttgttatcga tgcgaaactc aacgcgcaac actttacgcg gcagcgcagg gacgtaatga
    30241 atgataatgc ccttttgcgc gtcggtgact accggcacgg tgatttccgg ttttttgctc
    30301 tctttgttcg gcacgcgacc aaaggtgtcc gccgccattt ttgccaactc cggcagcggt
    30361 ttattactgt aaataaccgc cttcatcaaa ttggcggaat agtacttctc gtggaaatct
    30421 ttcagcgcct gctgcaccgg attaccaggt ttgtcgctta aagtttcgag gttaccacca
    30481 gaaaactttg aaccggggtg tgccgggtta atggtttctg cgctgacctg tgccatgcgc
    30541 atcccgtcac gcgtacgcgc catggttaat tcagcgttca ctgcattacg ctcacgttcg
    30601 gcgtatttct tgtcgagcaa gggttctgca atagcatcgg ccaggcggtc taccgcaccg
    30661 ggcaaggcgt cgttctcaac ttccagatag aaagccgtgc gatacggcgc agtgctggca
    30721 ttgtgactac cgccgtgcat tttgagatat tcggccagac tgtcagcctg cgggtacttt
    30781 ttcgacccca tcagactcat atgttcaagg taatgtgcca gcccctggta cgcctcggga
    30841 tcttccagcg acccaacggg cactaccagc gccgagagcg atttaactgc ctgcggatca
    30901 gaaaccagca agaccaccat accgttatcc agacgtatag cctgatactg gcggttatct
    30961 ttatcacttt tacggatggt ttcctgaatc ggctgccatc ccgtttctgc ctgacttaat
    31021 ggtgcccaga gggcaacaaa caacaataat gctttgaacc aggtgctgcg gggcattcac
    31081 ggacctcata atcaacttta ttttctggac agattcaaca ggttaacgca aatcatctgc
    31141 cagaatttaa tcttgtgctg cacgagtcag cctatgttta tataaccatc agtccgtgac
    31201 tggtgcgcat cataaagtaa gcagatagat tgcgcaattt ttatacaaca ctcatgactg
    31261 attaaagcga aacagcggta acaggaaacg ttgcgactgt tcaacgatgg cctccattgt
    31321 ctctggtgtt aattgccgcc agagccgttg ataccagata tcatcacctt cgccacgcac
    31381 catcatgttg ccttcgtaag cctgaaggaa tttcgtacgg gctttttgca acgtggaatc
    31441 gtcatccagc atggcatcgt tttgcgcgtc ataacaggtt tttagccacg cgccgccact
    31501 ttcaggtaac accagcaatg gcgcggacat tccttcacga tacccctcaa tcagttgtga
    31561 gaggtaatgc aaagcctgtt cggctgcaag cggcggaaaa cgccactcac cgtctttgcg
    31621 tagaaaaagg cgactttcgc cattaccacc gctggcacag tagacaaggt gttccagcca
    31681 aagttgcatt ccctgcgcca cacttaataa agagggacgc cagcgcaaca ggccatccgg
    31741 ctgcacctgc ggcaaccagc cagttatctg cacaccgttg caggcgagat caatttccat
    31801 actctgcccc ggctggcgac aggcaatgac tctgtcggca agctgctgca tctcctggca
    31861 ctgtgtttcc cagaaaattt caccaaaagc gccatacggt aaatcccctg ccgcccggaa
    31921 gcggcggaac aagcgttcgg catcatcctg ctcaaccagt gcattcaata actgctgatt
    31981 gatttgataa cggctaagtc cttccagaat aaatggctcg gtgtctggga tttcgctgtc
    32041 ttcagtatgg aagttcacct gcaaacgcat ctggaagaac gcccgcaccg gatgtgccca
    32101 gaatcgttgt agcgtttcca gcggcacggt ttccggtaag gtaaacggca gcggctgaac
    32161 aaattcagaa tgtgctttac cagcctggct ggccgcaggt agccattcac gagcatagct
    32221 ttgtcgttcg cctggctggt agttttgcgg atcaaacggc atccgggtat ggaggcaagt
    32281 aagatgcgct tttacccttg cctcgctttc atcacagttg agcgcttcat cgcccggtag
    32341 ataatgactt tgcccgatgt agtcgatcag ttcctgcacc agtaccgacg ggaaacgttc
    32401 actgttatcc tgaatggaac gcccgatata gctgatatag agtttttgct gcgcggaaat
    32461 taacgcttcc aggaacaggt agcggtcgtc atcgcgacgg ctacggtcgc cacgcttcgg
    32521 tttctggctc atcaggtcaa agcccaatgg cgcaagctga cgtggataaa cgccgtcgtt
    32581 cattcccagc aggcaaacca ctttgaacgg aattgaacgc attggcatca gagtacaaat
    32641 gttaaccggt ccggcgagaa aacgctggct gatacgttct tgatccagac gctgtgccag
    32701 ttcatcacgc aatagtgaca gcggcaccgc gtcgccatac tgcgcaccta aaccttcggc
    32761 gataatcgcc tgccattgtt gttcgatcag cgtcatcgcc gcttcggttt ccgcatccgg
    32821 caggaagaag gcgttgagca tatcgcgaca aaccggcaac cactcttcta gcggacgctc
    32881 ctgtgccagc ccgcgacgcc agatgtttag ctgcattagc agtgaagcca gatgccccac
    32941 cagttctgca attaagccgc tcgattcatc ataaggtaga accgattgcc actcgccctg
    33001 cgcgctctcc atcgcgtagc ccagcaacat acgcgtcagg ccaaatcgcc aggtgtgttg
    33061 tccggtggcg gggagttcca gctcgcgaac gttgtcgtca tctatccccc aacgaatgcc
    33121 ggattcgttg acccactggc gtaaataacg cagcccttct tcggtgatgt caaaccgcgc
    33181 cgccagcacc ggcacatcca gcagcgccag cacatcctct gacacaaagc gactgtcagg
    33241 cagtgataac aggctgataa acgcttccag taccggatgt gactgccgcg cacgacggtc
    33301 ggaaatggcg taaggcaggt aacgatccgc aggtgcgcta ccaaacactg cctgaataaa
    33361 cggactgtag ctgtcgatat cagccaccat cacgatgatg tcgcgcggag taagtgtcgg
    33421 ggcttcctcc agcatcgcca gcaagcgatc gtgtaaaact tcaacttcac gctgcgggct
    33481 atggcaaacg tggaatgtga tactgctatc cagtggatca agcgggcgtt tgttatcgct
    33541 acgggaaaac tcttcgatgt tcacacccgc aacggcgcgg ttttccagtt ccagaatgtc
    33601 agactgaatg ttatgcagca ggttatctgg cgtcacatcg acaaaagcgt ccagctcctg
    33661 gctgctctcc aggtcagaaa ggagataaat gtagtcgcgc ccaagcttcc cccatgaggc
    33721 cagcagcgag ttgccgacat cctgttcacc atcgctgtta aagagctgtc cggcattttc
    33781 gctatcgcga aatagcggca attcgcgatc ttcaaaactg tgtcgacgct gacgagtcag
    33841 cagtttcgcc agataagcag gatctttaat atcgccccag taataacggc aggggttggt
    33901 aaacaggaga tggatttcaa tatgtttacc cagcgcctgg agcgcctgga gataaacagg
    33961 cggtaacgcg gaaataccgc atataaagac gcgcgaaggt aaccccggcg ggcaggtcgt
    34021 cgcggactcc agcgtttcga taaagcgctg atagagattc gcgcggtgcc agcgcggttg
    34081 tccgagttca tgggtatatt ccaccagcgc cttccacaac ggggcttgcc aggcctgtgc
    34141 ttctcccagc ccttcaacca aatgtcctgt ttcccactgt gccagccagt ccggacgata
    34201 gaccagatac tggtcaaaca ggtccgccgc ttttgaggaa agctggaaca gttttcgctt
    34261 gtcgctatcg tcagtcagat aatgccgcaa cagggtaaag tcttcgcgct ccagcaattg
    34321 cggcagcaga gtcatcagtt tccagctcat gctctgtttg ttaaaggcgc tctctttggg
    34381 gatttccggt aacacccgga cgaacatatc ccagataaag ctcgctggca gcggaaaatc
    34441 aatgtttgcc gcaataccaa acttttgcga cagggtcatt tgcagccact gcgccatacc
    34501 ggtactttgc accagaatca tctctggttc gaaaggatcg tccagccgtt cgcgttcgac
    34561 aataaactcc atcaacgctt ccagcacgtc cagacgattg gaatggtaga cccttaacat
    34621 agcggctcct gactactgac aattcgggca atgcaggcgg gtcatctcgc cttctctgcc
    34681 cccgggtgaa actagcgtaa cgctgatgct gacacatccc gcctgcgatg tctgcattcg
    34741 gttgacctgc cagttggcag gtggcgaaat cgcgcgcagt tgcgtttgct gccaaccatg
    34801 ccgccagagc ttttggtact ggtttctgct ggcgagactg ttcattgatg ttcgctggat
    34861 acccgataac gcagtgacaa tcatcaccat cagcaccatc agcaccatca gcaccatcag
    34921 caccatcgca accattacct ccggcaggct aaagccttgt tgattcttca gggaagctga
    34981 cataatgccc cctctttcag cggacaaaaa tcgctccagc cgcgtggcga aaaaacaatt
    35041 tttccatcaa tgacttcgcc tgttcgccac aacgaaacgc cttcataacc ggcaatcaat
    35101 aaggcttcat tatctgccag taaacgcaaa caaacctggg catcggttcc agcgtactgc
    35161 gagcactgaa ctgctggctg cgtctgccag gagtgcattt ttccccacgc cagcgccgac
    35221 tgaacgatgg cctggcggcg caatgactga ctttccatgc tcacgcgaga ggcaaaactg
    35281 cgatcctgct gactcattcc ttgcaatagc aagctaccca aaaccagcag catcaggacc
    35341 agagccagtg acgaaacacc tttttcgcgg ttcacaggtt gaatcctgtc acgctatagc
    35401 tggcatccac cacggtttgc ggttcagact tactggcagc acgcatatta accgtcaaca
    35461 ccggcgagaa gccgctgaca tcctgacgta cgacctgaaa agtgtcgata atgatggcat
    35521 ccggattagt gactttatcc cagcccttac cttcacagga tgtcgcaccg cgtagcgttt
    35581 ccagcacatg ctccttcaga cgaaatccaa tctggtcgga ctcttttacc ggttcgcgat
    35641 cccagatacc gttactgttc gcatcccact gcacaatgat acagtcaccc tgtccgacaa
    35701 tttccagccc ttcgccggta cagctgccat gacaataacc cgccctctgg agatgcttcg
    35761 cgacggtaaa tacccgcagc cagatttcat cttccagtgc cagcttacga gtgctcgtta
    35821 aactttcacg ctgtaacgca ggcagaaagc gtgcagcccc cagcaacaat acgctactga
    35881 tcgccatagc aatcaacact tccagcagag aaaaaccttg ctcttttaca ggcatccttc
    35941 tgtttctcct tgctgataaa gccggagtct tccccacggc gaaaccacca gccaccactc
    36001 gcccgttgag tttttgaagc gaatatgccc ggcccatgcg gtattgcgca ggccaaagaa
    36061 agcaagcgaa ggtgtcaggt cgctcatttc gacttcgggc cagcgtggca caaagaccaa
    36121 tggtgaactg ccatgacagg tattggcccc agcagcggaa ctcacaaggc accataacgt
    36181 cccctccctg ataacgctga tactgtggtc gcggttatgc cagttggcat cttcacgtaa
    36241 atagagcaaa tagtcccgcg cctagctggc ggtttgccaa agccgttgcg actgctgcca
    36301 gtattgccag ccatagagtc cacttgcgct tagcatgacc aaaatcagca tcgcgaccag
    36361 cgtttcaatc agcgtataac cacgttgtgt tttcatgccg gcagtatgga gcgaggagaa
    36421 aaaaagacga gggccagttt ctatttcttc ggcgcatctt ccggactatt tacgccgttg
    36481 caggacgttg caaaatttcg ggaaggcgtc tcgaagaatt taacggaggg taaaaaaacc
    36541 gacgcacact ggcgtcggct ctggcaggat gtttcgtaat tagatagcca ccggcgcttt
    36601 aacgcccgga tgtggatcgt agccttcaat ctcaaagtct tcgaaacggt agtcgaagat
    36661 ggattcgggt ttacgtttga taatcaactt cggcagcgga cgcggttcgc ggcttaattg
    36721 cagatgagtt tgatccatat ggttgctgta caggtgcgta tcgccaccgg tccagacaaa
    36781 atcacccact tccagatcgc actgctgcgc catcatatgc accagtaacg cgtagctggc
    36841 aatgttgaac ggcaggccga ggaagacgtc acaggagcgc tgataaagct ggcaagagag
    36901 tttgccgtct gccacataga actggaagaa tgcatggcac ggtgccagcg ccattttatc
    36961 cagttcgcct acgttccacg ctgaaacaat aatgcggcgc gaatccgggt cgtttttcag
    37021 ctggttcagt accgtagtaa tctggtcaat atgacgacca tctggcgttg gccaggcgcg
    37081 ccactgttta ccatacactg gcccgaggtc gccattttca tcggcccatt cgtcccagat
    37141 ggtgacattg ttttcgtgta gataagcaat gttagtatcg ccctgcagga accacagcag
    37201 ttcgtggatg atggaacgca ggtggcaacg tttggttgtc accagcggga atccatcctg
    37261 caggttaaaa cgcatctgat gaccaaaaat ggaaagcgtt ccggttccgg tacggtcgtt
    37321 tttctgtgtg ccttcgtcga gcactttttg catcagttct aaatactgtt tcatggttcc
    37381 tcaggaaacg tgttgctgtg ggctgcgacg atatgcccag accatcatga tcacacccgc
    37441 gacaatcatc gggatggaaa gaatttgccc catgctgatg tactgcaccc aggcaccggt
    37501 aaactgcgcg tcgggctggc ggaaaaactc aacaatgatg cgaaacgcgc cgtagccaat
    37561 caggaacaag cctgagacag ctcccattgg gcgcggttta cgaatataca ggttgaggat
    37621 aataaacagc accacacctt ccagcagcag ttcgtaaagc tgtgatgggt ggcgcggcag
    37681 cacaccgtaa gtgtcgaaaa tggattgcca ctgcgggttg gtttgcagca gcaaaatatc
    37741 ttctgtacgg gagcctggga acagcatggc aaacgggaag ttcgggtcaa cgcggcccca
    37801 caattcaccg ttaataaagt tgcccagacg cccggcacca agaccaaacg gaatgagtgg
    37861 tgcgataaaa tcagagacct ggaagaagga acgtttagta cggcgggcga agataatcat
    37921 caccacgata acgccaatca ggccgccgtg gaaagacatg ccgccgtccc agacacggaa
    37981 cagatacagc ggatcggcca taaactgcgg gaaattgtag aacagaacat aaccaatacg
    38041 tcccccgagg aagacgccga ggaagcccgc atagagtaag ttttcaactt catttttggt
    38101 ccagccactg cccggacgat tcgcccgacg tgttgccagc cacattgcaa aaatgaaccc
    38161 caccagatac atcaggccgt accagtgaag cgccacgggt cctattgaga aaatgaccgg
    38221 atcaaactcc ggaaaatgca gatagctact ggtcatctgt caccacaagt tcttgttatt
    38281 tcgctgaaag agaacagcga ttgaaatgcg cgccgcaggt ttcaggcgct ccaaaggtgc
    38341 gaataatagc acaaggggac ctggctggtt gccggatacc gttaaaagat atgtatatga
    38401 tccgcgctat aaccctccgc gaatcagccc gcccatgccg cgacgctcca taaaggctgc
    38461 aacctgatga cgaacttcgg tcgccagttg cgcttccaga ctacgctgcg caagattttc
    38521 tgcttcggca aaatcaatgc gccgcagcag gtattttacc cgcgctacag aacgtccgtt
    38581 catagacaga tggcgatacc caagcccaat gaggattgcc acgcacatgg gatcgcccgc
    38641 catttcaccg cacaaacgaa gatcgatacc atgtatttcc gcttcccggg cgatcatcgc
    38701 cagagctcgt aacattgcag gatgaagact gtcgtaaatg ttcgccaccc gggtattgtt
    38761 gcgatcaacg gccagaatgt attgagtcag atcgttggtg ccaacagaga tgaaatcgac
    38821 cctctttgcc agatgcggca gcataaatac cattgacggc acttccagca tgatgccgat
    38881 acgtggtttg ggaatttcgt aaccgatcat ctcctcgact tcacgtccgg cacgttcaat
    38941 caggcggcgt gcttcgtcaa cttcatcgag gctggtgacc atcggcaaca gaatattcag
    39001 gttgcccgta gcggcattag cacgcagcat cgcccgcacc tggatcaaga agatctccgg
    39061 ctgatcgagc gtaatgcgaa tcccacgcca acccaggcat ggattctctt cgctgatcgg
    39121 catgtaaggc agctgcttat ctgctccgac atccagcgta cgcaaggtga cgggtttatc
    39181 attgaacatt tgcagcatcc cctgatactg cgccacctgt tcttcttccg acgggaaacc
    39241 actttgcagc atgaatggga tttcagtgcg ataaagaccg atgccatcaa tacagctgcc
    39301 cagtttttct tcatgttccg ggcttaaacc agcattgagc atgactttta tacgctcacc
    39361 gctttttaac tgcgcgggta aattgacgtc atcttccgcc agacggctaa gctcaatctc
    39421 ttcactaatt agccgctgat attcttgcag cagtaccggc tccggatcga ccagcaattc
    39481 accgcgatag ccatcaacga tcagcgtcct acgatgcagc accgaaggct gaatatccgc
    39541 gcccatcacg gtagggatcc ccagcgcacg taccatgatc gcagcatggg agttggctgc
    39601 gccatctcgc acgacaacac cgactaagcg atcctggggc agctcagcaa gagttgtcgc
    39661 tgacagttca tctgccacca gaatgaaacg ttccggccag gcgttcggcc cttgattagc
    39721 gtcatcaaga tgaaacagca atcgctgacc cagcgcacgt aaatcaccag cccgctcttt
    39781 gagatagtta tcgcttagcg cggcaaactg ttcggcaaat ttttcaatga ccgtttttac
    39841 cgcccactct gccaccgagc ctttatcaac ctcggcaaac aattcgcgac gcagccgggt
    39901 gtccgaaagc aggtgcgagt aaagatcgaa aatagccgcc gtttcttttt gtgctccggc
    39961 ggcaaagcgt ttgctgtagc ggcgaaactc gtttgccgct tcttccagcg ccccggtcag
    40021 tcgttcgcgt tccagagccg gatccagcgt tgatgcctga tacacctgtt ccattaaagg
    40081 taacgtggca tcctgccagc cttcggcaat cgccacacca ggtgctgccg gtaatgcgcg
    40141 gattcgcgtc tggcgatatt gcccaaacag ggcagtcaac tgcgactgag aaagaatagc
    40201 tgccatctgg gtggcaagcg tcaccaggaa ggattcttca ctttcgtcat actggcgcaa
    40261 ctctcgttgc tgtaccacca gtacaccaag caactggcga cgttgaataa ttggtacgcc
    40321 taaaaacgcg cggaaacgtt cttcttttac ggaggggatg tatttgaagc tggggtgctt
    40381 ttgcgcatct gcaaggttta tcggttccgc cagcctgcca accaggccga cgatcccttc
    40441 atcaaacgcg agcgttacag tgcgaccgcg tggttttttc agcccccggg tcgccatcag
    40501 gtagtaacaa cgtcgatcat gatcggccag gtagaccgaa cagacctcgg tatccatcgc
    40561 aagacagatg tcggtaacca gaatatttaa cgcctcattc aggcgtggtg cgctggctac
    40621 cttttcgact atttcgcgca ggcgagtgag cataattggc gtgacttaac ctcttttacg
    40681 tcgataagca gatgcgtttt gtggttttgg cgtattttcc tgcagtgaca tcaccacact
    40741 cgcgaactct ttcattaccc tacggtagac atcacgttta aatgacacca cctgtctgac
    40801 cggataccag taacttaccc atcgccagcc gtcaaactct ggtgtactgc tggtttgcat
    40861 attgatttct gcatcgccgc ttaccagctg caagagaaac catttttgtt tttggccgat
    40921 acaaaccggc ttcgtgtccc aacgcaccaa acgtttcggt aatttgtagc gcaaccagtt
    40981 acgcgttgaa gcaaggattc gaacgtcttt gcggcttaat cctacttctt caaacaattc
    41041 acggtacatc gcctgctctg cggattctcc ggggttgatt ccgccttgcg gaaattgcca
    41101 ggagtgctga ccaaatcgcc gggcccacat tacctgcccc tggcgattac aaatcacgat
    41161 acctacgttt gggcggtagc catcgtcatc aatcaccgga ctacctcaaa ataaagcttt
    41221 atatacgaat gattgtttca tactccagga agacggtaaa ccactctctg cagggcatta
    41281 cacactaata acaattgaat aactcacagt tatgtgcaga gttataaaca gaggaagggg
    41341 tggatagccg tttttattca ccttttctgt ggatagagtt gtgaagaact acggaattac
    41401 tacgggaaaa cccggagcat tctgaataag cccgatatag acattttaaa atattcatta
    41461 ttagtcaatg cattacatgt aatttgagta atgaaaaaga gtaattcgtg acccaggtca
    41521 cacctctcat ttacgggttg gcgaaagatc aaccaatgcc gtgtttatcc acagaatgtg
    41581 ccactaagtt aagcactgaa ccactaaaaa ctggagtttc gtcgcacgtc aaggctgtaa
    41641 atgaaaacag tagtggaggt ttttcacagt tatcccagct ttctgtggat aacatggtgt
    41701 aagatcctgt ttattttcag tgaccagatt tggaaaacct attgcagtgt tgcgcaactc
    41761 gattaccggc agcctaaaaa gcaatataaa tcagagaatt gaacaacaca tgtggaaaaa
    41821 gttacactgc gaatattcgg cacataattg ctgtttgttt tttaaccaag gtatcatgac
    41881 atgtcccaac ctcgcccact gctctctcct cccgaaactg aagaacagtt gttagcgcaa
    41941 gcacagcaac tttctggtta tacattggga gaactggcgg cacttgacgg gctggttacg
    42001 ccagagaatt taaaacgcga taagggctgg attggcgtgt tactggagat ctggctaggt
    42061 gccagcgcag ggagtaaacc tgagcaagat tttgctgctc tgggcgtgga acttaaaact
    42121 atccctgtgg atagtcttgg tcgtccgctg gaaacaacat tcgtttgtgt tgcccggtta
    42181 acgggtaata gcggggtgac ctgggaaacc agccatgtgc gccacaagct caaacgcgta
    42241 ctgtggatac cggttgaagg cgagcgcagc atcccgctgg cgcagcgtcg cgtaggatca
    42301 ccgttgctgt ggagcccgaa tgaagaggaa gaccgacagc tacgcgaaga ctgggaagaa
    42361 ttaatggata tgattgttct cggtcaggtt gagcggatca ccgctcgtca cggggaatat
    42421 ttacagataa gaccgaaagc agcgaatgcg aaagcgctta ccgaagccat tggtgcccgg
    42481 ggcgaacgga ttctgacgct gccacgcggc ttttatttga agaagaattt caccagtgca
    42541 ctactggccc gtcattttct gatccagtag ccatcgcttt gacctgccgc tttccgggca
    42601 tataattacc gcttcatttt tttggcaggg ctttttagat gttatttgca tggataaccg
    42661 atcctaacgc ttggcttgcg ctcggtacgc tgacgctgct ggagatcgtt cttgggatcg
    42721 acaatattat tttcctttct ctggtggtgg caaagcttcc cacagcacaa cgtgctcatg
    42781 cgcgccgtct ggggttggcg ggagccatgg ttatgcgtct ggcgctgctg gcatcaatct
    42841 cttgggttac gcgcctgaca aatccgcttt ttacaatatt cagtcaggaa atttctgccc
    42901 gtgatttgat tctgcttctg ggtggcttgt tccttatctg gaaagccagc aaggaaatcc
    42961 acgaatccat tgaaggtgaa gaagaagggc tgaaaacacg cgtttcatca ttcctcggcg
    43021 ctatcgtgca gattatgctg ctggatatta tcttcagcct cgactcggtg attaccgctg
    43081 tgggtctgtc agatcacctg tttattatga tggcagccgt ggtgattgcc gtaggcgtga
    43141 tgatgttcgc cgcgcgctcg attggtgatt ttgtcgaacg ccatccttcg gtaaaaatgc
    43201 tggcgctctc tttcctgatt ctggtgggct ttaccctgat tctggaaagt ttcgacatcc
    43261 acgtaccgaa aggttacatc tacttcgcga tgttcttctc tattgcggtt gaaagcctca
    43321 acttgattcg caacaaaaag aatccgctct gatactccgt acgctctcct gcgggagggc
    43381 gtactcactc cttttaccct cacagtttaa gattttcctg ctttcaagat atataacgtc
    43441 ggtttataaa cagactatta tcataggtag gccaaatgag gataaccgga tgaaaaaatg
    43501 ggcagtaata atttctgcag tcggactggc gtttgctgtt tccgggtgtt ccagtgatta
    43561 cgtcatggcg accaaagatg gccgtatgat tttgaccgat ggaaaacctg aaattgatga
    43621 tgataccggg ctggtgagtt atcacgatca gcaaggtaac gcgatgcaaa ttaaccgtga
    43681 tgatgtttcg caaattattg aacgttaaca aataaggtca gcatccggct ggccttaaga
    43741 tttttctctt tcccttttcc cttccctctg ccatttttat attccttatg tcgtgattat
    43801 aaaaaggaaa cggctatgca atatcaccgt ataccccaca gttcgctgga agtcagcacg
    43861 ctggggcttg gcacgatgac gtttggtgaa cagaacagcg aagccgacgc ccacgcacaa
    43921 ctcgactatg ccgtcgctca gggcattaac cttatcgacg ttgccgaaat gtacccagta
    43981 cctccgcgcc ccgaaacgca agggttaacc gaaacctacg tcggcaactg gctggcgaaa
    44041 catggcagcc gcgaaaagtt aattatcgcc tccaaagtga gcggaccgtc gcgcaataat
    44101 gacaagggca tccgcccgga tcaggcgctg gatcggaaga atatccgcga agcgctgcat
    44161 gacagcctca agcgcctaca gactgattac ctcgatcttt atcaggtgca ctggccgcag
    44221 cgcccgacca actgcttcgg caaactcggt tatagctgga cggattctgc gcctgcggtt
    44281 tcgctgctgg atacgctgga cgcactggca gagtaccaac gcgcgggaaa aattcgttat
    44341 atcggcgtgt cgaacgaaac tgcatttggc gtaatgcgct acctgcatct ggcggacaaa
    44401 cacgatctgc cgcgtattgt caccattcag aacccttaca gtctgttaaa ccgcagtttt
    44461 gaagtaggtc tggcagaagt cagccagtat gaaggggtcg aactgctggc ctattcgtgc
    44521 ctgggtttcg gcacgctgac cgggaaatat ctcaatggtg caaaacccgc tggcgcacgt
    44581 aatacgctct ttagtcggtt cacccgctat agcggtgagc aaacgcaaaa agccgtcgcg
    44641 gcgtatgttg atatcgccag acgtcatggc cttgatcctg ctcagatggc gctcgcgttt
    44701 gtacgccgtc aaccgtttgt tgccagcact ctgctgggcg caaccacgat ggatcagctg
    44761 aaaactaaca tcgaaagttt gcatctggag ttaagcgaag acgtattagc tgaaattgaa
    44821 gcggtacatc aggtttatac ttatcctgca ccataaagat taacgccggt taaaaccggc
    44881 gttaaatatt aatgacggcg ctgccagatc cacagcgccg ttattgccag cgcaaatagc
    44941 gcaccgaagc caatgccaat gggcacgacc gggatgccta ccattaccgc cagcgagtaa
    45001 atgcccagca tcaacaacat ggcgctgttt tcgccaaggt tttgtactgc aatcgcattc
    45061 cccgccccga cgcttttttt accccgctcc tgtagcaacg cattgagcgg aacgacaaaa
    45121 aagcccccca gcacgccaat cagcatcaac aaggcatagg ctggcagcag ctcgtgttgc
    45181 agggagaaaa tcggtaccac cacgccaatc aaaatcccgg ctggcataca gcgtgacacg
    45241 gtttccagcg taaccagctt cgctgccgca cctgcgccaa ccacgatacc aatcgctacc
    45301 atcgcgttga gataggtcgg cgtagcgtta tcggtaatgc ccagcgccac cggtacccac
    45361 agcaccaaca ggaaacgcag cgtgacaccc gctccccaga ataaactggt gcccaccagc
    45421 gaaaaacgcg tttcaccatt gcaccatagc gaggtgcagg cattcaggaa actgcgggtc
    45481 atgttgatga gattccagga ctgccccgga cgcgccgcca gtttgggaat gtagatattg
    45541 gcaacgaccg caccaccgta ggccagtgcg catgcggcca gagcgaccag gacatgccag
    45601 tcagccagca caccaccggc tacggaaccg agtaaaatcg ccgctatggc agaagcttcc
    45661 attaaaccgt tagcttttac taacttacta cccgtggtta attcgccgag aataccgtat
    45721 ttcgccggtg aataggctgc agcaccaaca cccaccagcg tatagccgag aaacggattg
    45781 ataccaaagc agatactggc tgcgcccagc agcttcaggc cgttggcaaa catcatcacc
    45841 cggcctttgg cgaagctatc cgccacctgc ccgacaaacg gcgccaaaag aatgtaagca
    45901 cctacaaaca ccatttgcag gatgggctgg ctccactccg gatagaactg cgctttcagt
    45961 aacgccagag tggcaaacag tagcgcgtta tcgccaaacg cagagagaaa ctgcgccacg
    46021 ataaccgctt tcatcccctt cgaccacaac gaagtgttag tgtgcactga ctcactcatc
    46081 gtgttgttcc gcttcgtcta cccagctttt caacgtgaca aagtcaggtt tgccgctgcc
    46141 aagtaatggc atctgtttca gatagcgaat atcgcgcggt acagcaagct ccggcacgcc
    46201 gtgctcgcgg gcatactgtt gcaacttatc gcgcgtcagt tcgttatctg tggtgaaaag
    46261 caccagtgcc tcgcctttgc tggcatcgct cttaatcgca gtggcatgga ctttatctgg
    46321 cgaaacacca agtgccagtt gttccaccat ttccagcgac accatttcgc ctgcaatttt
    46381 ggcaaagcgt tttgcgcggc cctgaatctg cacaaagccc tgctcgtcaa aacgcacaat
    46441 atcgccagtg tcataccagc cgcgctccat ttcgccgcga acattctcgg cggtgggcac
    46501 ttccagtaca cctggcttct ccacccgcag atagccgttc attatgttcg gccctttcag
    46561 ttgcaggcgt ccgccctctt cgataccagg gaccgacaac aggcgtgcat ccattcctgg
    46621 tagaatacgc cctaccgtac cgggtttcgc cgccatcggt acgttgatag aaacgacagg
    46681 cgcgcattcg gtcacaccgt agccttcaag gatgcgcagg ccaaatttat cctgccaaag
    46741 ctgtttggta ctttcttgta gtttttctgc gcctgccacc acatagcgta gacgatagaa
    46801 gtcatacggg ttggcgaaac gcgcgtagtg accgaggaaa gtcgaggtac cgaacaacac
    46861 ggtgcaactg cggtcataca ccaagtccgg cacaatgcgg taatgcagcg ggcttggata
    46921 aaggaacact tctgcacctg taagcagtgg cgtaaacagg cctaccgtca gcccaaagga
    46981 gtgaaacagc ggtaatgccg acataaagcg atcgttggtg gtgaagtcgg caatcgtttt
    47041 aatctgctcg acgttcgcca gaatgctttt atggctatgg acgacgcctt ttggatggcc
    47101 ttcagaaccg gaggtaaaga ggatcagcgc ctcttcttcc ggctgctgtt ttacctgcgc
    47161 caggcgcggc atcagcaaat gagcgaagat ccatactttg tcggcagtgg tgacatctgc
    47221 ttttaaatct tccagataga cccagcgcac ctgagtaagt tgctccggca gatgccagag
    47281 tttgccttta tcgagaaact ggcgggaagt gaagatggtt ttgatttcag ccgccgtaat
    47341 agcactggtc agccctttta ccccggcagt gtagttcatc attgcgggaa tgcggcgacg
    47401 cgcgatggcc ccaaaaatca ctgccgcact gatgcctgca ttgggcagca ttaagccgat
    47461 gcgttcgcct tcaacactgt atttttcaag gatgcgtcca acaaacagcg ttttcgtaag
    47521 caatttgcga taggagtctg gggtaaagtt gacgtcttcg acacatttct tcccggctcc
    47581 gaagcggtac attgcactca gtaaagattc gtacagagtt tcacgcgggc gcaccgccat
    47641 acgcgcttcc atcattattt gatgcagcat ttcgccagcg attttgcgac ggtcacgggc
    47701 acgcggcgca tccggcatcg ccacctgcgt tggaggcaaa atatgcagag taatttgcgg
    47761 gaacaagcga cgtttaacca gacctttcag gcggctgaag tgcgtaagtt ccgccccttc
    47821 aatacgcaca ggaataaccg ttgcaccaga cttcgccgcg acaaaacccg cgccatcgta
    47881 gattttcatc agcgagcctg tagtggtgat gcgtccttca gggaaaatca ccactggtcg
    47941 gccctgttcc accagacgta ccagatgttt aatagccata ggttgcgtcg ggtcgagagg
    48001 aacaaagtcg ataaatgatt tcagccaacg catataccac tgttggctta ttgaggtgta
    48061 aacggcaaac actggacgca caggtaaaaa cagtccaagc aaaatgccat caataaaaga
    48121 gacgtgatta ggcgtaatta gtacgcgctc gcccttcagt gcctgggtgt cacccgtaac
    48181 gcgaacgcga tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca ttccaactcc
    48241 ctttgctctg attcagtaaa agcgaatgga gggagattac acgagataaa gaacgcgagc
    48301 gacagtaaat taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg caagagacag
    48361 ggtacgaaga gcgtaccgaa taatctcacc aatcaatacc tctgggatct tgattgtggt
    48421 ctgcacgacg actcttcgcc agcgagaaaa cgcaaaggaa tgaagggaaa tgcaacgagg
    48481 tgtgtaaatt gtcggttact gttacagatt gatgaccggc aaaaaaaaac ctgcgcatct
    48541 gcgcaggctg gtgtaattca tgtgctcaac acgaagttga cttcacctat caatacctct
    48601 gggatcacca ctttagcaac ctgaagccaa acgccaccag cggtcaatcg caacagcgtt
    48661 tcgcaaagtg taaccaaagg tttgcaatct ctttttttgt cttacttatt tgcgtttggc
    48721 tcacattccc acgatgaaaa cacgccaccc cttgaaccaa cgggcgtttt ccgtaacact
    48781 gaaacaatgt aagcgtttac ccactaaggt attttcatgg cgaccataaa ggatgtagcc
    48841 cgactggcag gcgtttcagt cgccaccgtt tcccgcgtca ttaataattc acccaaagcc
    48901 agcgaagctt cccggctggc tgtgcatagt gcaatggagt ctcttagcta tcacccgaac
    48961 gccaacgccc gtgcgctggc gcagcagacc actgaaacgg tcggtctgat cgttggtgat
    49021 gtttccgatc cgtttttcgg tgcaatggtg aaagcggtcg aacaggtggc ttatcacacc
    49081 ggtaatttct tattgattgg caacggttac cacaacgaac aaaaagagcg tcaggctatt
    49141 gagcaactga tccgccatcg ctgtgctgcg ttggtcgtcc atgccaaaat gatcccagat
    49201 gccgatttag cctcattaat gaaacaaatg cccggtatgg tgctgatcaa ccgtatcctg
    49261 cctggctttg aaaaccgttg tattgctctt gacgatcgtt acggtgcctg gctggcaacg
    49321 cgtcatttaa ttcagcaagg tcatacccgc attggttatc tgtgctctaa ccactctatt
    49381 tctgacgccg aagatcgtct gcaagggtat tacgatgccc ttgctgaaag tggtattccg
    49441 gccaatgatc ggctggtgac atttggcgaa ccagacgaaa gcggcggcga acaggcaatg
    49501 accgagcttt tgggacgagg aagaaatttc actgcggtag cctgttataa cgattcaatg
    49561 gcggcgggcg cgatgggcgt tctcaatgat aatggtattg atgtaccggg tgagatttcg
    49621 ttaattggct ttgatgatgt gctggtgtca cgctatgtgc gtccgcgcct gaccaccgtg
    49681 cgttacccaa tcgtaacgat ggcgacccag gctgccgaac tggctttggc actggcggat
    49741 aatcgccctc tcccggaaat tactaatgta tttagtccga cgctggtacg tcgtcattca
    49801 gtgtcaactc cgtcgctgga ggcaagtcat catgcaacca gcgactaacc gcagttaaag
    49861 caattccagc gccagtaatt cttcgatggt ctggcgacgg cgaatcaacc gcgcctgacc
    49921 attatcaaac agaacttctg gtaacagcgg acggctattg tagttggatg acattgatgc
    49981 gccatatgcc cctgtatcat gcagtaccag ataatcacct gccttcactt ccggcaaggc
    50041 gcgggtttca acatttcccc cttcctgctg ggtaaagaca tcgcccgatt cacataacgg
    50101 cccggcgacg acggtttcca ccgttggcgc gtgttccaga gaacgaccat cagctgccag
    50161 ggcactgata tggtggtaac taccgtacat tgccgggcgc atcagatcgt tgaacccggc
    50221 atcaaccagc acaaagtggc ggctacccat ttgtttgacg ctccgcacct gagtaattaa
    50281 tacgccagac tgcgctacca ggaagcgacc cggttcaatt tccagtttca cagggtggcc
    50341 caaatggcga gcgatttgct cacgcgcggc attccacaga ccataataat gttcggtatc
    50401 aaccgcctct tcaccctgtt gataaggaat agaaagccca ccgcccgcag aaatagcctg
    50461 taaatcctga ccgaattcga taacctgacg caccatagca ccacacacct gctccagatg
    50521 ggcataatca acgccagaac caatgtgcat gtgaatgccg actagctgca gatgatgacg
    50581 ttgtatcacg tccagtgcgg cgggcagatc ggtgtaccag ataccgtgct tgctgttttc
    50641 gccaccggta ttggtttttt ggctatgtcc gtgaccaaac cccggattaa cgcgcagcca
    50701 tacccgatgc cctggcgaaa cctggcccag ttggtcgagc gtatcaacag aacccgcatt
    50761 caccggaatt tgcaattcac tgacgcgttc aagcgtcgcc tgatcgataa catctgccgt
    50821 aaaaacaata tcatcggggt gcgtttgcgg attgtaaccc gccgccaacg cacgctctat
    50881 ttcgcctaac gagacggaat ccactttcac gccctgctca cgcattaagc gcaaaatatg
    50941 aatattggaa caggctttct gtgcaaagcg caccacttca aactgtttca gcgctgcaat
    51001 ctgccgacga ataatttgcg catcgtagac ccacaccggg cagccaaatt cagcgggcaa
    51061 acgcagcaga ttttcggcgg tgagatcggt atcggtgctg aacagtgaat gtggcataac
    51121 aaactccaga tgagtgcttt tttatgatta cgccacatca taaaaagaat aaaaaatatc
    51181 gatttatgtc gagtctatgc aaaaatgata tgaattaccg gattgcgaga gagcgctaat
    51241 ggccgccgtt aacttacgtc atattgaaat ttttcatgcg gtaatgaccg ccggaagcct
    51301 gactgaggca gcacacctgc tacacacctc acagccaacc gtcagccgcg aactggcgcg
    51361 ctttgagaag gtgatcgggc tgaaattgtt tgagcgcgta cgtggacgat tacatcctac
    51421 cgtgcaagga ctgcgtctgt ttgaagaagt gcaacgatcc tggtacggac tggatcgcat
    51481 tgtcagcgcc gcagaaagtc tgcgcgagtt tcgccaggga gaactgtcta ttgcctgcct
    51541 gccggtcttt tcgcaatctt ttttaccgca gctcctgcaa ccctttctgg cacgttatcc
    51601 cgatgtcagc ttaaatatcg tgccccagga atcaccgcta cttgaagagt ggctctcggc
    51661 ccagcgtcat gatttaggac tcactgagac actccatacg cctacgggaa cagaacgtac
    51721 cgaattactc tctttagatg aagtgtgtgt gttacctccg ggtcatccgc tggcggtaaa
    51781 aaaggtatta acgccggatg attttcaggg tgagaattac atcagccttt cccgtactga
    51841 cagctatcgc cagttgctgg atcagctatt tactgaacat caggttaaac gacgcatgat
    51901 cgtagaaacc cacagcgccg cgtcagtctg cgcaatggta cgggcggggg taggtatttc
    51961 ggtggttaac ccgctcaccg cactagatta tgcggcaagc ggtttagtgg tgcggcggtt
    52021 cagtattgcg gttccgttca ccgtcagcct gatccgcccc ctgcaccgcc cgtcatcagc
    52081 gctggttcag gcgtttagtg ggcatttaca agcggggtta ccgaaactgg tcacttctct
    52141 tgacgctatt ttgtcgtcag ctacgacagc ataaaagcga cagcatcctc ggcatggatc
    52201 gccgcggtat caaacacagg caaaacactg cgctcttctg gcaccagtaa accaatttct
    52261 gtgcagccaa aaatgacgcc ctgtgcgccc tgttctgcaa ggcgagcaat cacttgcgca
    52321 taataagcgc gtgacgcttc ggtaaattgc cccagacaca gttcttcaaa aataatctga
    52381 ttaattttcg cccgttcatc cgcttcagga ataagacaat tgatggaaaa ttgttccgtc
    52441 agccgcccgc gataaaaatc ctgttccatg gtgtaacgcg tacccagcag cgccacacga
    52501 gtcattccgg ccccggtaat tgcacgtccg gtggcatccg caatgtgtaa gaaaggcaga
    52561 gtgcaacgtg actcaatggc atccgccact ttatgcatcg tattggtaca taacacaata
    52621 ccttctgcgc ccgcccgctg taagccaagc gccgcctcag ccagaatgtc cccggtttta
    52681 tcccattccc cgcgacgctg gcactcttct atttcatgaa aatcgacgct atgtagcagc
    52741 atttgcgcag agtgaagccc accaagccgc tgtttaatgc cttcatttat cagacgatag
    52801 taaggaatgg tggattccca gctcattcct cccagcaaac caattgtttt cattcctctc
    52861 tcctgatgtg tgttaccgca acaaggctga ataaggaacg agacaaacgc ctcaacggcc
    52921 aggtgcccaa tctctattaa cgaaaaaagg gccggatgta cagcacatcc ggcccgtgaa
    52981 atcagacgcc gatatttctc aacttctcgc ctgccatcag tttgcgttcg atatgttcca
    53041 gcgtgacatt tttggtttcc ggaatgagcc agaaagtaat acccacaaac gcaatgttca
    53101 gcgcagtgta gagccagaac gtaccggcag cgccaatgct atcaagcagt gtcaggaagg
    53161 ccgcgccgat aatcatattc gacacccagt ttgtcgtcgt cgaacaggta ataccgaaat
    53221 cgcggcattt cagcggctga atttcagagc acaggatcca caccactggc gcggcgctca
    53281 tcgcataacc ggcaatacac atcatcgtca tgccaacaga gagccaggac aagccactgg
    53341 aagccgtacc gttatcaaac tgcatcaggc aatagcccag caccagagtg cctaacgcca
    53401 tcacgctgaa accaattttc agagccggtt tacgacctgc tttatctacc gtaaagaccg
    53461 caatgaaggt cgcgaacata aaggtcagtc ccacgaccag agtcgcaatc atctgttgtt
    53521 ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc gtagtacatg atgatgttca
    53581 taccggtaaa ctgctgcatc gcctgcaaca acataccgag aaacacagca cgacggacgt
    53641 tacggttgat cttaaacagt gcccaaccgc cctgttttaa cttcaggctt tcacgaattt
    53701 cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag catacgcaac acttcttccg
    53761 cctcaatatg acgccccttt tccgccagcc agcgcgggct atttggcagg aagaccacca
    53821 gaataatcag cagaactgct ggtaaagcaa gaacccccaa cattgcgcgc cagttaccgc
    53881 tataactgaa cgctgtatcg gataaaaacg ccagcacgat gccgagtgtg accatcaact
    53941 ggtacatact gatcatctta ccgcgaacgt tttcacttgc catttcagaa agatacagag
    54001 gagcggtgta agacgcgatc ccgacagcaa tgcccagcac cacacgagcg gcgattaaca
    54061 tctctacgct ggtcgcaaaa gcggacccta tagaaccgag tacaaacagg atggcccccg
    54121 ccatcaggct gtatttacgc cccaggcgga acgacagcca accattaaac agcgcaccaa
    54181 ttgctgcgcc gagcatcatg ctactaacca cccattcctg caaacgactg gtcagcacaa
    54241 agtgatcggt aatgaacggc aacgctccgg cgattacgcc gatatcaaga ccaaataaca
    54301 atcctgcgac cgcagcagct accgaaacaa acatattcat acgccgcgta tcacgcaaag
    54361 aacgtggcgt taaagcagat tccgtattga tagtaaccat tttttcctgc cagcagagag
    54421 taagacattg tgaaaaaata cgtgaacaat tcacacaggt gtcaggtcgg aaacagcata
    54481 aatatggatt aaattgctgc gacatgccgt tatgtgatgg atattccaat tttaaaatta
    54541 agttgaatta ttgagattat tattaacctc ctaattttaa agcagataaa attcataaag
    54601 ttcattaatt gataattgat atggattatt tcataaccat gatatggatt atgatgatct
    54661 agagatataa aaaaccctgc catgcggcag ggtcataaaa gtaagaggaa tgaattaacg
    54721 cgccagccaa ccgccatcca cagcaatggt ataaccattt acataatctg aagcgctgga
    54781 ggcaagaaac actaccggcc ccatcaggtc actcggcagt ccccaacggc cagctggaat
    54841 gcgatcgaga atttccgcgc tacgttgttc atctgcccgc agttgttggg tattgttggt
    54901 cgccatgtaa cccggagcta tcgcattaac attaatgttg tgtttagccc attcgtttgc
    54961 catcaatcgc gtcacaccca tcacgccgct ttttgatgcg gtataagaag gcacacggat
    55021 cccgccctgg aaggagagca ttgacgcgat attgataatc ttgccgccat tgccttgcgc
    55081 gataaagtgt ttcgccgctg cctgagacat gaagaatacg ctcttgatat tcaggttcat
    55141 gacatcgtcc cagtcttttt cgctgaactc gagagcgtct tcgcggcgaa tcaatccggc
    55201 gttattcacc aggatatcaa tatgaccaaa ttcagctacc gcgcgatcca gcagtccagg
    55261 aatgccatca atctttcgca gatcggcggt caggcttaaa aaacgacgcc ccagcgccgt
    55321 gacctgctcg atggtttcag tcggttcaac gatgttaatg ccaacaatgt cacagcccgc
    55381 ttgcgccagc cccaacgcca tcccctggcc cagcccagta tcacaaccag tgacgaccgc
    55441 aactttacct tggagagaaa atgcacttaa aatcataaca acaccttatt cgtttatgcc
    55501 cacaactagc gcaaatcttt aacgcccaca tggtccatat cattaaagac ctggttttca
    55561 ccgaccatgc cccagataaa ggtataagct ttggttccga cgccggaatg gatcgaccag
    55621 ctcggggaga tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc ttgcggctgc
    55681 cccatcatgt ggaaaacgca ggcgtcatca tccatattga aatagaaata aacttccatc
    55741 cggcgctcgt gggtgtgaca cggcatggtg ttccacaagt tacctggagc cagctccgtc
    55801 agccccatac tcaattgaca ggtttccagt acatccggga caaaatattt gttaatcgtg
    55861 cgacggttac tggtgaggtt atcgcctaac gtgactggag atacttcgtc cggtgtgact
    55921 tttttggtgg gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt cgccggagtg
    55981 gcggtatcaa tactggcaaa gacaacttct tttgcacctt taccaacata cagggcgtcg
    56041 cggtgaccga tttcatagca ttgaccatcg acagtaatcg taccggcacc gccaatattg
    56101 ataacaccta actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt accaacttcc
    56161 ccgccaacgg aaaccgtttt agttatcggc ataatgccgc caacaataat tcggtcaatg
    56221 tggctgtaaa ccatggtgta ctcatcggcg acaaatacct tttcaaccaa aaattcattg
    56281 cgcagccctt gggtatccag cgtttttgcg tgcgcaccgt ggatgctctg tcttacgtcc
    56341 acatcaacct ccgataatca gtgaagcgtg aaaatagaaa acgatagtga aacactgttt
    56401 cgttttctct ataaagggat gatatgccca tgggctaggg ttttcaatta aaaataaaac
    56461 aatgttttat ttttaattta aggattaaaa aaagtgcagt gtcgatcacg aatatgtcat
    56521 gagaatggaa aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc atcattattc
    56581 aacagattaa tgacggcaga tatttatcgg taaggaggaa atgcagagct actcgtcacg
    56641 ttcaatggtc aatgccacgc cctggccccc gccgatacaa agcgttgcca gccctttgcg
    56701 ggcattacgt ttcaccattt catgaaccag agaaaccagg attcggcaac cggaagcgcc
    56761 tatcgggtga ccgagtgcga tcgcgccacc attgacattg acccgacgct catcccactc
    56821 aagcatcttg ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct cgataagatc
    56881 gacatcagcc aactgccagc ctacacgctc caggcaacgg cgggtcgcat acaccggcgc
    56941 aattcccatc aatgccggat ctacaccaac gctggcaaat gcgcggatgc gggccagcac
    57001 gggtaaattc aacgctcgtg ctttggcttc gctcatcatc attaccgcag ctgcgccatc
    57061 gtttatggat gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg aaggatttaa
    57121 acgggctaaa ccttctgcac tggcgtcagt gcgtggctgt tcatcggtat caacaaccaa
    57181 cgtctgaccg ttactttgga tcattaccgg gacgatctca tctttaaatc gtccggcgtc
    57241 aatcgccgct cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca actgacggct
    57301 gatgccatat tcgcgagcca gattttcggc ggtgacacca atatgataat cattgaaggc
    57361 atcccacaac ccatcatgca ccagactgtc aaccaactgg ctattgccaa gctgttcacc
    57421 ggtgcggcta tcagtcagaa catgtggtgc gcggctcatg ttttcctggc cacctgcgat
    57481 gacaatatca gcctcgccac actgtatcgc ctgagtagcc agatgcagtg ctttaagacc
    57541 ggaaccgcaa acgtcattaa tcgtgattgc agaaacgctg ttaggaagac cacctttaat
    57601 agccgattgc cttgccggat tctgccctgc ccctgcagtc aacacctgac caagaattac
    57661 ttcatccacc gcatatgcag gaacgccggt acgttctatt aacgctttca cgaccagact
    57721 accaagttcc acggcggaat gacccgctaa cgcaccacga aagcagccga taggtgtccg
    57781 taacgccccg acaatcacaa cgtctttcat cacaacctcg caccaattaa cagtgcaata
    57841 gtaaatgatt gttaattaca gttatcttaa ttgtttaaaa aaagtgattt ttatcacaaa
    57901 ggaaatatgc ctgagcagta gtcagagtca taactggcac gtaaggtttg caaccactaa
    57961 cccaccaata gatgggtaga tagggcgtta atctcccata cttaacttgg tttatggtaa
    58021 attgccctcc attttgttta atttgtagat gatacgttca gataatgtct aatatttggt
    58081 ctaaagaaga aactctgtga agtttcgcgc tctatggcac agccgttgat gcaggcacgc
    58141 tattccttcc tattcagtta ggttcggcag gggctgtggt cctgtttatt actgctctgg
    58201 tcgcctggcc tttaacatat tggccacata aagccttatg ccagttcatc ctctcatcaa
    58261 aaacatcagc aggtgaaggg ataacgggcg cggtaacaca ctactatggc aagaagattg
    58321 gtaatctgat taccacgctg tacttcatcg ccttttttgt cgtcgtgttg atatatgcag
    58381 tggcaattac caactcactt acggaacagc tggcaaagca tatggttatt gatcttcgca
    58441 tccgtatgtt ggtgagtctg ggtgttgtat taattctgaa tctcattttt ctgatgggac
    58501 gtcatgccac tattcgggta atgggatttt tggtattccc attgattgcc tatttcttat
    58561 ttctttccat ttaccttgtc ggtagttggc aacctgatct attaacaacc caggtagagt
    58621 tcaatcagaa tacccttcac cagatatgga tatcgattcc cgtgatggtt ttcgccttta
    58681 gccatacgcc cattatttct acgtttgcca tagacagacg tgaaaaatat ggcgaacacg
    58741 ctatggataa atgcaaaaaa attatgaaag tcgcttatct catcatctgc ataagtgtac
    58801 tgttctttgt ctttagctgc ctgctttcta ttccatcttc gtatattgaa gcggctaaag
    58861 aagaaggggt caccatttta tcggcgcttt ctatgctgcc gaacgcccca gcatggttgt
    58921 caatttccgg gattattgtc gcagtagttg cgatgtcgaa atcattcctg ggtacgtact
    58981 ttggcgttat tgaaggtgcc acagaggtcg tcaaaacaac actacagcag gttggtgtaa
    59041 agaaaagtcg tgcatttaac cacgcactat caattatgtt ggtatcgctg attaccttca
    59101 ttgtttgttg cattaacccg aacgcgattt cgatgattta cgcgatcagc ggcccgctca
    59161 ttgccatgat acttttcatc atgcctacgc tgtcaacgta tctcatcccg gcgcttaaac
    59221 cctggcgttc catcggaaat ctgattacgc tgatcgtggg tatcctgtgc gtatcggtaa
    59281 tgttctttag ctaatatccg ttgtggatgg ttcaaaattg ctacaatttt gaaccattcc
    59341 atttttcata atattcattt gttattattc cgccctatca cttctctcgt catatcctag
    59401 aattatcagt tagttaaaca cacaaagaga tctgaacatc ttaaataaga ctatttaaga
    59461 tgcataactt agattcgcaa gatatcttct ggtcattcaa aaacaatttg cgatatagat
    59521 attctcatac catgagatag ttatctggac cacgttcgac catgcaagat taaatcagtg
    59581 aaacataatc atatttaata acgagaaatg catttttaaa tatcttaatc agaattatag
    59641 caaatacaga ttaatcgcaa tttttcccag caaaattaca aaatcaaggt gtattgacat
    59701 catcaatcac caatgaaatt atgcaattat atacggatag ggaggttctt aacatggggc
    59761 tttgtagtcg ttataaaagt cttacatgca atagttgctc aatgcattgc caaataatgc
    59821 cagaagagtc accgcgtttg cagtactgcg caaactcgtg tt
//
LOCUS       DAETQL010000014        58573 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.014, whole genome shotgun sequence.
ACCESSION   DAETQL010000014 DAETQL010000000
VERSION     DAETQL010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.014"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1100)
                     /locus_tag="K0W65_000907"
     CDS             complement(<1..1100)
                     /locus_tag="K0W65_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HBI3094281.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="K0W65_000908"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="K0W65_000908"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBI3094282.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="K0W65_000909"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="K0W65_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBI3094283.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="K0W65_000910"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="K0W65_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBI3094284.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="K0W65_000911"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="K0W65_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBI3094285.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="K0W65_000912"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="K0W65_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBI3094286.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="K0W65_000913"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="K0W65_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBI3094287.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="K0W65_000914"
     CDS             complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="K0W65_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HBI3094288.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="K0W65_000915"
     CDS             complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="K0W65_000915"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HBI3094289.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="K0W65_000916"
     CDS             complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="K0W65_000916"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HBI3094290.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="K0W65_000917"
     CDS             complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="K0W65_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HBI3094291.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="K0W65_000918"
     CDS             complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="K0W65_000918"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HBI3094292.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10283..11701
                     /gene="puuA"
                     /locus_tag="K0W65_000919"
     CDS             10283..11701
                     /gene="puuA"
                     /locus_tag="K0W65_000919"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HBI3094293.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11850..12014
                     /locus_tag="K0W65_000920"
     CDS             11850..12014
                     /locus_tag="K0W65_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094294.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12004..13389
                     /gene="puuP"
                     /locus_tag="K0W65_000921"
     CDS             12004..13389
                     /gene="puuP"
                     /locus_tag="K0W65_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HBI3094295.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13523..13768
                     /locus_tag="K0W65_000922"
     CDS             13523..13768
                     /locus_tag="K0W65_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBI3094296.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14081..15724
                     /gene="sapA"
                     /locus_tag="K0W65_000923"
     CDS             14081..15724
                     /gene="sapA"
                     /locus_tag="K0W65_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBI3094297.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15721..16686
                     /gene="sapB"
                     /locus_tag="K0W65_000924"
     CDS             15721..16686
                     /gene="sapB"
                     /locus_tag="K0W65_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBI3094298.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16673..17563
                     /gene="sapC"
                     /locus_tag="K0W65_000925"
     CDS             16673..17563
                     /gene="sapC"
                     /locus_tag="K0W65_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBI3094299.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17563..18555
                     /gene="sapD"
                     /locus_tag="K0W65_000926"
     CDS             17563..18555
                     /gene="sapD"
                     /locus_tag="K0W65_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBI3094300.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18557..19363
                     /gene="sapF"
                     /locus_tag="K0W65_000927"
     CDS             18557..19363
                     /gene="sapF"
                     /locus_tag="K0W65_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBI3094301.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19431..19784
                     /locus_tag="K0W65_000928"
     CDS             19431..19784
                     /locus_tag="K0W65_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HBI3094302.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20152..20940
                     /gene="fabI"
                     /locus_tag="K0W65_000929"
     CDS             20152..20940
                     /gene="fabI"
                     /locus_tag="K0W65_000929"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBI3094303.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTRESFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21084..22211
                     /locus_tag="K0W65_000930"
     CDS             21084..22211
                     /locus_tag="K0W65_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HBI3094304.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22279..24213
                     /gene="rnb"
                     /locus_tag="K0W65_000931"
     CDS             22279..24213
                     /gene="rnb"
                     /locus_tag="K0W65_000931"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBI3094305.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24291..24394)
                     /locus_tag="K0W65_000932"
                     /pseudo
     CDS             complement(24291..24394)
                     /locus_tag="K0W65_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24448..26422
                     /gene="pdeR"
                     /locus_tag="K0W65_000933"
                     /pseudo
     CDS             24448..26422
                     /gene="pdeR"
                     /locus_tag="K0W65_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26570..26743
                     /locus_tag="K0W65_000934"
     CDS             26570..26743
                     /locus_tag="K0W65_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094306.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26833..27582
                     /locus_tag="K0W65_000935"
     CDS             26833..27582
                     /locus_tag="K0W65_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI3094307.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27851..28069
                     /gene="osmB"
                     /locus_tag="K0W65_000936"
     CDS             27851..28069
                     /gene="osmB"
                     /locus_tag="K0W65_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBI3094308.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="K0W65_000937"
     CDS             complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="K0W65_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBI3094309.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="K0W65_000938"
     CDS             complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="K0W65_000938"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBI3094310.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="K0W65_000939"
     CDS             complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="K0W65_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBI3094311.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="K0W65_000940"
     CDS             complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="K0W65_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBI3094312.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="K0W65_000941"
     CDS             complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="K0W65_000941"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBI3094313.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32018..32608
                     /gene="ribA"
                     /locus_tag="K0W65_000942"
     CDS             32018..32608
                     /gene="ribA"
                     /locus_tag="K0W65_000942"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBI3094314.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32672..35347)
                     /gene="acnA"
                     /locus_tag="K0W65_000943"
     CDS             complement(32672..35347)
                     /gene="acnA"
                     /locus_tag="K0W65_000943"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBI3094315.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35511..35606)
                     /locus_tag="K0W65_000944"
     CDS             complement(35511..35606)
                     /locus_tag="K0W65_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094316.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35720..35887)
                     /locus_tag="K0W65_000945"
     CDS             complement(35720..35887)
                     /locus_tag="K0W65_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094317.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35890..36054)
                     /locus_tag="K0W65_000946"
     CDS             complement(35890..36054)
                     /locus_tag="K0W65_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBI3094318.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36349..37323)
                     /gene="cysB"
                     /locus_tag="K0W65_000947"
     CDS             complement(36349..37323)
                     /gene="cysB"
                     /locus_tag="K0W65_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBI3094319.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37533..40130)
                     /gene="topA"
                     /locus_tag="K0W65_000948"
     CDS             complement(37533..40130)
                     /gene="topA"
                     /locus_tag="K0W65_000948"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBI3094320.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40510..40761
                     /locus_tag="K0W65_000949"
     CDS             40510..40761
                     /locus_tag="K0W65_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBI3094321.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="K0W65_000950"
     CDS             complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="K0W65_000950"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBI3094322.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42066..42824
                     /locus_tag="K0W65_000951"
     CDS             42066..42824
                     /locus_tag="K0W65_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBI3094323.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42821..43411
                     /gene="cobO"
                     /locus_tag="K0W65_000952"
     CDS             42821..43411
                     /gene="cobO"
                     /locus_tag="K0W65_000952"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBI3094324.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="K0W65_000953"
     CDS             complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="K0W65_000953"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBI3094325.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44424..45044)
                     /locus_tag="K0W65_000954"
     CDS             complement(44424..45044)
                     /locus_tag="K0W65_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBI3094326.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45041..45922)
                     /gene="yciV"
                     /locus_tag="K0W65_000955"
     CDS             complement(45041..45922)
                     /gene="yciV"
                     /locus_tag="K0W65_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBI3094327.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46060..46104
                     /gene="trpL"
                     /locus_tag="K0W65_000956"
     CDS             46060..46104
                     /gene="trpL"
                     /locus_tag="K0W65_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HBI3094328.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46081..46174
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46196..47758
                     /gene="trpE"
                     /locus_tag="K0W65_000957"
     CDS             46196..47758
                     /gene="trpE"
                     /locus_tag="K0W65_000957"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBI3094329.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47758..49353
                     /gene="trpD"
                     /locus_tag="K0W65_000958"
     CDS             47758..49353
                     /gene="trpD"
                     /locus_tag="K0W65_000958"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBI3094330.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49354..50715
                     /gene="trpCF"
                     /locus_tag="K0W65_000959"
     CDS             49354..50715
                     /gene="trpCF"
                     /locus_tag="K0W65_000959"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBI3094331.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50727..51920
                     /gene="trpB"
                     /locus_tag="K0W65_000960"
     CDS             50727..51920
                     /gene="trpB"
                     /locus_tag="K0W65_000960"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBI3094332.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51920..52726
                     /gene="trpA"
                     /locus_tag="K0W65_000961"
     CDS             51920..52726
                     /gene="trpA"
                     /locus_tag="K0W65_000961"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBI3094333.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53106..53285
                     /locus_tag="K0W65_000962"
     CDS             53106..53285
                     /locus_tag="K0W65_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBI3094334.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53371..53871
                     /locus_tag="K0W65_000963"
     CDS             53371..53871
                     /locus_tag="K0W65_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBI3094335.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53917..54423
                     /locus_tag="K0W65_000964"
     CDS             53917..54423
                     /locus_tag="K0W65_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HBI3094336.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="K0W65_000965"
     CDS             complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="K0W65_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBI3094337.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55361..55450
                     /locus_tag="K0W65_000966"
                     /pseudo
     CDS             55361..55450
                     /locus_tag="K0W65_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55478..56221
                     /locus_tag="K0W65_000967"
     CDS             55478..56221
                     /locus_tag="K0W65_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBI3094338.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56251..56790
                     /locus_tag="K0W65_000968"
     CDS             56251..56790
                     /locus_tag="K0W65_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBI3094339.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56895..57293
                     /gene="yciA"
                     /locus_tag="K0W65_000969"
     CDS             56895..57293
                     /gene="yciA"
                     /locus_tag="K0W65_000969"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBI3094340.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57333..58061)
                     /gene="tonB"
                     /locus_tag="K0W65_000970"
     CDS             complement(57333..58061)
                     /gene="tonB"
                     /locus_tag="K0W65_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBI3094341.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            58276..58572
                     /locus_tag="K0W65_000971"
     CDS             58276..58572
                     /locus_tag="K0W65_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HBI3094342.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        14063 a        15145 c        14644 g        14721 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaataatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggaagggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccatgtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg agctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaaaaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataacttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgctctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
     4921 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
     4981 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
     5041 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
     5101 ggcaaccagc tcttttggcg caacgttgaa accgcactcg ccctgcaccg gttcgaaaat
     5161 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
     5221 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
     5281 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
     5341 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
     5401 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt
     5461 agcctgcccg ctcactgggg caagggtgtt aattttctcc gccagcgtga cgtagctttc
     5521 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
     5581 gaccagatca gggtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
     5641 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
     5701 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
     5761 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
     5821 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
     5881 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca
     5941 gcagttcagc aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac
     6001 cctgcatgta atagatgttg gtatcaaggc ggccgaactg cggcatgtgc gacaaggtca
     6061 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt
     6121 ttagtagttt cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta
     6181 cacccccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
     6241 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
     6301 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta
     6361 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat
     6421 tggcagtccg aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat
     6481 acacgcgccc gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt
     6541 gaatatgccc accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc
     6601 ggcgaatagc gttgacgtcc agcagctcca gctgtttatt gccgtagcgt acccagttct
     6661 ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg
     6721 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct
     6781 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
     6841 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
     6901 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
     6961 aggagagccc ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca
     7021 gtgtgtcgaa tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
     7081 tcatttcagg cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca
     7141 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
     7201 tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg
     7261 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
     7321 gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa
     7381 atctcttcgc ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg
     7441 gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca
     7501 cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
     7561 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
     7621 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgcg cacctggccc
     7681 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
     7741 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta
     7801 tcacccgcgt ctttcagcag ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca
     7861 tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg
     7921 ttcaacacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
     7981 agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg
     8041 agtttccagc aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg
     8101 accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac
     8161 actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca
     8221 cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc cagctcttcg
     8281 gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt agccggagaa
     8341 gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc acggtcgata
     8401 tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg atcaacggtt
     8461 tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa gcggttttca
     8521 atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt cattatgact
     8581 cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag agatccccgg
     8641 gctgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc attgcgctta
     8701 cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc
     8761 aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt
     8821 gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca ccttccagta
     8881 ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt
     8941 acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg
     9001 acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat
     9061 caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct
     9121 tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt
     9181 gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt
     9241 ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg
     9301 tagtggtcag agtcgctgtt tttcagcgat atggtgctgc caagcggtga taaagccctc
     9361 gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc actgtacgcc
     9421 cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc
     9481 ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag agtttaccca
     9541 aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc
     9601 atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat gttccagcaa
     9661 ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca ccagttcttg
     9721 taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat taattatcgc
     9781 catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt caccatatag
     9841 gtgcggctgc acattgctgg gactactagg aagataaatg ccatcgagtt tcggcaaaag
     9901 ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca agccgcctgc
     9961 gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac ccttaagcct
    10021 gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca tgctcaatct
    10081 cacaaaatgg actaaattat cgccattact gcgctttaaa tgacctttat gttcaatatt
    10141 ttttcaatct agcagtggat tttcattttt gcaaactcaa tttaacattt gacaaacatt
    10201 tggtttgcat acagattcga atgatggtca ttatatttta cgctttgata acgagcggaa
    10261 aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga actttgttca
    10321 gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct acctggagcg
    10381 ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt gcttccgtgg
    10441 aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt tcccggcctc
    10501 ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg gtcaggaaat
    10561 gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt ctgccgccga
    10621 tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg ctccctttga
    10681 cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc gcggattgtt
    10741 cccggttgta gcggtagagc tggagttcta tttactggat cgccagcgcg acgctgaagg
    10801 ggatctgcaa ccgccctgcg cgcctggcac cgatgaccgc aatacacaaa gccaggttta
    10861 ctccgttgat aacctcaacc acttcgccga cgtgctcaat gatattgatg aactggcgca
    10921 gttgcagctg attccggcag atggcgcggt cgctgaggcc tcgccgggtc agtttgaaat
    10981 caacctttac catactgata acgtgctgga agcctgcgat gatgcgctgg cactaaaacg
    11041 cctagtgcgt ctgatggcag aaaagcataa gatgcacgcc acttttatgg cgaagccgta
    11101 tgaagagcac gcgggcagcg ggatgcatat ccatatcagt atgcaaaata atcgtggcga
    11161 gaatgtgctt tctgacacag aaggcgaaga ttcgccgctg ctgaaaaaga tgctcgccgg
    11221 gatgattgac ctgatgccgt cgtcgatggc gttgctggca ccaaacgtga actcgtatcg
    11281 ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg tggggccata acaaccgcac
    11341 cgtcgccctg cgtattccgt gcggtgaccg tcataatcac cgcgtggaat atcacgtggc
    11401 gggtgccgat gccaacccat atctggtgat ggcagcgatt tttgccggta ttttgcatgg
    11461 ccttgataac gagctgccgt tgcaggaaga agtcgaaggc aacgggctgg aacaggaagg
    11521 cttacccttc ccgattcgcc agagcgatgc cctgggtgag tttatcgaga atgatcacct
    11581 gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac gcctgtaaaa atgatgagct
    11641 gttgcagttt gagcgcctca tcacagaaac agaaattgag tggatgttga aaaacgcgta
    11701 attccccttg cggggttgtt tcatcggcct gagcggagcc aggcctgaca tttgctggta
    11761 atccagacat ttcccggatg tcgcgttatg gagcgcaggc ggcaacgggc ttccgttaac
    11821 gattaagact gtgacgagga aatgagatga tgccgatgat taaatccccg catggcgagg
    11881 gaggctgcgt atgcgcccct cctgctactg actggactcc gccccctttg cttcccttgc
    11941 taaacaggtt tgatttcagg tcgacacgac cgcaaacctt attacgcagg ggaggcagca
    12001 attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga
    12061 aaatcactga aattgtggca ggtggtaatg atgggtctgg cctatctcac gccgatgacc
    12121 gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat
    12181 ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag
    12241 tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaaccc gcacgtcgga
    12301 tttatggtcg gctggtcatc actgctggat tatctctttt tgccaatgat caacgttttg
    12361 ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtggta
    12421 accttcgtcg ccattttaac cgccgcgaat ctgaagagcg tcaacctggt cgctaacttc
    12481 aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtggtt
    12541 cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc gtttatcagc
    12601 gagaacgcgc acctgatccc gattattacc ggtgcgacga ttgtctgttt ctcgttcctc
    12661 ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg cgtgatcccg
    12721 aaagccatct tcctgacggc ggtatacggc ggcgttatct ttatcgcggc gtcgttcttt
    12781 atgcagctgt tctttcccga tatcagccgc tttaaagacc cggacgccgc actgcctgaa
    12841 attgcgctgt acgtcggcgg caagctcttt cagtcgattt tcctctgcac cacgtttgtg
    12901 aacacattag cgtctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg
    12961 gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa atggcggact
    13021 ccggcactca acgtcattat ggtcgggatt gtcgcgctgt cggcgctgtt ctttgattta
    13081 gtcaccgcga cagcactgat taacttcggt gctctggtgg cgtttacctt cgtgaatctg
    13141 tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc
    13201 cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaaccta
    13261 gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc atatttgtgg
    13321 tatctgatcc gccgctatcg caaagtgccg ctgtacgaag gtgacagaac gccggtgagc
    13381 gaaacgtaat agccggatgc gcggcgcatt atcctgcctg agggtaacgc gccgcgccat
    13441 taactataaa aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc
    13501 gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc
    13561 cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag gctgaacgca caccgctggc
    13621 ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc
    13681 ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga
    13741 gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg tcagtatcgt
    13801 tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg cagcgtgctt
    13861 taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg tgagttatag
    13921 ttatagagaa atcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc
    13981 aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat
    14041 attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc
    14101 tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc
    14161 gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt
    14221 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga
    14281 tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg
    14341 ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca
    14401 aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt
    14461 ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta
    14521 cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac
    14581 cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta
    14641 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca
    14701 actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca
    14761 atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt
    14821 agtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg
    14881 cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt
    14941 gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc
    15001 gccgctaaat aatcccgctg tccgccatgc actggcactg gcgattaata accagcgcct
    15061 gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc
    15121 gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca
    15181 gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca
    15241 ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt
    15301 tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc ggttgatgga
    15361 tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt
    15421 cttccgtccg ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg
    15481 caatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcagcgcg
    15541 tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattaccta ttctgccgct
    15601 ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc
    15661 gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc
    15721 atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc
    15781 ttcgttggct tcagcttgag ctatttcact ccacacgcgc cgttacaagg cgcgtcactg
    15841 tggaatgcct gggtgttctg gtttaatggc ctgatccact gggattttgg tgtttccagc
    15901 attaacggtc aaccaattgc cgaacagtta aaagagctct tcccggcgac gatagaactg
    15961 tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg
    16021 attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca
    16081 atcccggtgt tctggctggc gcttctgttg acactgtttt gttcactcac gctaggttgg
    16141 ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg
    16201 ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc
    16261 cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc
    16321 ctgatgcgta tcagcactat tgaagtgtat gaccagaact atgtaaaagc ggcggcgacc
    16381 cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc gttaccgccg
    16441 gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat gatcaccgaa
    16501 atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg ccagcaggat
    16561 tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg
    16621 atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta
    16681 cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt
    16741 ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcgggactgg ctgtactgtg
    16801 tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg gttatcaatt
    16861 attgccgcca tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct
    16921 cggacgtgat gttttaagcc gtttactgag cggagctgcg ccaaccgtgg gtggcgcatt
    16981 tgtggtgacg cttgccgcga cgatctgtgg cctggtactg ggcaccttcg ccggggcgac
    17041 acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg cgatcccttc
    17101 gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc acgctatgtt
    17161 tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca tggtgcatga
    17221 cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat
    17281 tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga tcacccgcgc
    17341 actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcacaact
    17401 cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc
    17461 gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg ttaacctcct
    17521 tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt actcgatatt
    17581 cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt cgaccgggta
    17641 agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag
    17701 agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt tactgctgac
    17761 cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaaactg
    17821 gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa
    17881 cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg
    17941 cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa
    18001 gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa
    18061 gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgaca
    18121 aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa
    18181 aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg
    18241 gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag taaggagttg
    18301 gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc
    18361 agcgcgatgc cgcataaaag tcgcctcaat actctacccg gcgctatccc attgctggaa
    18421 cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg agaatgcatt
    18481 gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac
    18541 atggagaaag agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt
    18601 ccgctaccgg accggttggt ttcgtcgtca gaccgtagaa gcggtaaaac ccttgagctt
    18661 tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac
    18721 actggcaaaa atgctggcgg gaatgataga acctaccagc ggcgagttat tgattgacga
    18781 tcatccgctg cattttggcg attattcctt ccgtagtcag cgtattcgca tgatttttca
    18841 ggatccttcg acctcgttga atccccgtca acgtatctca caaattctgg attttccact
    18901 gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg
    18961 tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg cacccggaca
    19021 aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga
    19081 tgaagccctc gcttcactgg atatgtcaat gcgttcgcag ttgattaatc tgatgctgga
    19141 gttacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg gaatgatgaa
    19201 gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc gaggcagcac
    19261 cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg ccgggcactt
    19321 tggcgaagca ttgacagcgg atgcgtggcg taaagatcgc taattttctg aatcctcctt
    19381 ccagattatt cacctcattc tgacaagaga ttaaatcttg tcagaatgag gtgatggata
    19441 aaataaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa cgaaagctct
    19501 ggcgatatct tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa catccggtgg
    19561 ggagctacat tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag ctggatggtg
    19621 ggcagcatga tttggcagtt gcctatgata ccaggcgcac tagctggctt gagtcgcagg
    19681 gctggaccgt gctgcgtttc tggaataacg agattgattg taatgaggag gcggtgctgg
    19741 agattattct gcaggaactg aaccgccggt caccctcacc ctgaaagggc gagggggccg
    19801 accgagccga atagctgttg tggtgaaaac atggagacag tgctggagaa tattcggcaa
    19861 ggtctgaacc atcccagcca tcgccataaa agggttaggg gctgtatgag cctgtttgtt
    19921 gctggggtaa caatatttgc acaatatggt cccctcgccc ctctggggag agggttaggg
    19981 taaggggaaa agcgcccccc ctgccgcagc ctgctccggt cggacctggt aactatagct
    20041 actcacagcc aggttgatta taataaccgt ttatctgttc gtactgttta ctaaaacgac
    20101 gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt
    20161 ctttccggta agcgcattct ggtaaccggt gttgccagca aactgtccat cgcctacggt
    20221 atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa
    20281 ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt tctgcagtgc
    20341 gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa agtttggccg
    20401 aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct ggatggtgac
    20461 tatgttaacg ccgttacccg tgaaagcttc aaaattgccc acgacatcag ctcctacagc
    20521 ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc cctgctgacc
    20581 ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa
    20641 gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt
    20701 gttaacgcca tctctgctgg tccgatccgt actctggcgg cctccggtat caaagacttc
    20761 cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtacagt tactattgaa
    20821 gatgtgggta actctgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa
    20881 gtagtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga actgaaataa
    20941 tcgttctgtt ggtaaagatg ggcggcgttc tgccgcccgt tatctctgtt atacctttct
    21001 gatatttgtt atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt
    21061 tcctttacga ccaaggagcg cccatggaac aacgccacat caccggcaaa agccactggt
    21121 atcatgaaac gcaatccagt actacggagt atgacgttct gcctctggtc ccggaagccg
    21181 caaaggtcag cgatcccttt ctgctcgacg tgatccttga tgaagagacg ctggccccct
    21241 tcctttcatg gctggtccct gcgcgcgttc ttgcagtgga gttgttccct gaccagctta
    21301 ccgtgacccg ttcacaaacc ttcaccgctt atgaacgctt gtcgacggcc ctgacggttg
    21361 ctcaggtttg cggcgtccag cggttatgta actactattc ggcgcgactt acgccgctcc
    21421 ccggacctga ttcctccagg gaaagtaatc atcggttggc acaaatcacg caatatgccc
    21481 gccaactggc tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg
    21541 tcggtcttac tgtctgggac tgtgtgatca ttaaccaaat cattggtttt attggctttc
    21601 aggcgcggac aattgcgaca tttcaggctt atctcgggca tccggtacgc tggttacccg
    21661 ggctggcgat acaaaactac gccgacgcgt cactgtttgc tgatgaatca atacgctggc
    21721 gaagcagcta tgaggtggaa aaactacctg aagagcacac aaaaagttca actgcagaac
    21781 tttgccaact ggccgaaaca ctctctctcc accctatttc actttccctt ctcgaaaagt
    21841 tgttaaacag cacacgggtc aatgcacagc cggataatca gcttgcggcg ttgttatgcg
    21901 cgcgtataaa tggcagtcct gcttgttttt ccacctgtat ggattcatca aatgaatata
    21961 aaaaaatcag cacccttctg cgcaagggcg aaaatgaaat taaccgatgg gctgaccgtc
    22021 attctgttga gcgcgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct
    22081 ttagcgccgc ccagttcagc cctttactcg aacacgaaaa atcatcaacg cagattatta
    22141 atctgctggt atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg
    22201 agacatatta accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg
    22261 ccaccaaaat agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa
    22321 ctgcattccc agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc
    22381 ttcctggaag tcgacgcaca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc
    22441 atgcatggcg accgaattat cgcggtgatc cacagtgaaa aagaacgtga atccgcagag
    22501 ccagaagaac tggttgaacc gttcctgact cgtttcgtgg gtaaagttca gggcaaaaat
    22561 gaccgtctga ccatcgttcc tgatcatcca cttttaaaag acgccattcc ttgccgcgca
    22621 gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg cggttgccga aatgcgccgt
    22681 catccgctga aaggcgatcg ttctttctat gctgaactga cacaatacat cacttttggt
    22741 gacgaccact ttgtaccgtg gtgggttacc cttgcgcgcc ataatctgga aaaagaagca
    22801 ccagacggcg tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgacctcg
    22861 ctggattttg tcaccatcga cagtgccagc actgaagata tggatgacgc ccttttcgct
    22921 aaggcgctgc cggatgacaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg
    22981 attgctgaag gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctg
    23041 cctggcttca acatccctat gctgcctcgc gagctttctg acgatctctg ctcactgcgc
    23101 gccaatgaag tccgcccggt actggcatgc cgcatgacgc tctccgctga tggcaccatt
    23161 gaagataata tcgaattctt tgccgccacc atcgaatcca aagcgaagct ggtgtatgac
    23221 caggtttctg actggctgga aaataccggt gactggaagc ctgaaagtga agcaattgcc
    23281 gaacaagtcc gtttgctagc gcaaatttgc caacgccgcg gcgagtggcg tcataaccac
    23341 gcactggtgt ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg
    23401 ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga agcgatgatt
    23461 gccgctaaca tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg catctataac
    23521 gtgcatatgg gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct gaaaacgcac
    23581 ggtctgcatg tcgatgccga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt
    23641 gaactggatg cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt ccagtcattt
    23701 gctgaaatta gctctgaacc cggtcctcac tttggcctcg gtctggaagc atacgccacc
    23761 tggacttcgc cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt
    23821 atcaaaggcg aaactgcgac gcgtccgcag gatgaaatca ctgtccaaat ggccgaacgt
    23881 cgccgtctca accggatggc agaacgtgat gttggcgact ggttatacgc acgcttcctg
    23941 aaagataaag ccgggaccgg cacccgtttc gcggcggaaa ttgtcgatat cagccgtggc
    24001 ggcatgcgtg tgcgtttggt tgataacggc gctatcgcct ttattccggc acctttctta
    24061 cacgctgtgc gcgatgaact ggtttgcagc caggaaaacg gcactgtaca aattaaaggt
    24121 gaaacggttt acaaagtaac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa
    24181 acccgcagca ttattgcgcg cccggtcgcg taatctcctt tcacggccca ttcctcatgg
    24241 atgggccgtt tatttccccg ctctaccttc gttatatcca ggcagatttt ttaactgact
    24301 ttcgtttgaa aactggcgtt tttccaaaac tggattacaa ataaatatat aaaaaaacaa
    24361 tgcgtttagc cgccgttaat ttcatgtaag tcatgttgtt ccttttattt aaccgcggac
    24421 tccgctgtta accggaggat atgcatcatg aaaaccgtta gggagtccac aacgttgtac
    24481 aactttctcg gatcgcacaa tccatactgg cggttgacgg aaagcagcga tgctttgcgc
    24541 ttttctacca ccaaaaccac agaacctgat cgtacattgc agttatctgc cgaacaggct
    24601 gctcgcatca gggaaatgac ggtcatcacc tccagcctga tgatgagtct gaccgtcgat
    24661 gaaagcgatc tttctgtgca tctggtagga cgaaaaatca ataaacggga atgggctggc
    24721 aacgcgtctg catggcatga cacaccggcg gttgctcgtg atttatcaca cgggctttcc
    24781 tttgctgagc aggtagtttc tgaagcacat tccgcaatag tgattctcga cagccggggg
    24841 aatatccaac gcttcaatcg gttatgtgaa gattacacag ggttgaaaga acacgacgtc
    24901 attgggtaaa gcgtgtttaa actgtttatg agccgtcgtg aagctgcggc atccaggcgc
    24961 aataaccgtg tattttttcg aagcggcaat gcatatgaag tcgaactgtg gataccaaca
    25021 cgtaaaggcc agcggctgtt tctgtttcgc aataaatttg tccacagcgg cagtggcaaa
    25081 aacgagattt ttttaatctg ttccggtacc gacattaccg aagagcgccg cactcaggag
    25141 cgactgcgta ttctggcaaa taccgacagt atcaccggac tgccgaatcg taacgcaatg
    25201 caggagttaa tcgatcacgc tattaatcag gcagataaca ataaagttgg ggttgtgtat
    25261 cttgatttgg ataattttaa aaaggtcaac gacgcctatg ggcatttgtt tggtgaccag
    25321 ttattacgcg acgtgtcatt ggctatttta agctgtctcg aacatgacca ggtgttggcg
    25381 cgtccaggtg gcgatgagtt tctggtactg gcatccaata cctcacaaag cgcgctggaa
    25441 gcaatggcat cacgaatttt gacccgctta cgcctcccct ttcgtattgg tttaattgaa
    25501 gtttatacca gctgttcagt aggtatttcg ctctctcccg aacatggttc ggacagcgcg
    25561 actattattc gtcacgccga cacagcaatg tacacagcga aggaaggcgg acgaggacaa
    25621 ttttgcgttt ttactccaga aatgaatcag cgggtatttg aatatctctg gctggatacc
    25681 aacttgcgta aagcactgga aaacgatcag ttggttatcg aaaatcacct agcgtgtcga
    25741 agtgcgcagt ctggaagcac tagtacgttg gcagtcacct gaacgtgggt tgattccacc
    25801 gttggacttc atttcctacg ccgaagagtc agggctaatt gtgcctttag gccgttgggt
    25861 gattctcgat gtcgtacgcc aggtggcaaa gtggcgggat aaaggcataa acctgcgagt
    25921 ggcggtaaat atttctgcac gtcagctcgc cgatcaaacc attttcaccg ccctgaaaca
    25981 ggttctccag gaactcaatt ttgaatactg ccctatagat gttgaactga cagagagttg
    26041 tctgattgag aatgatgaac tggcactgtc tgttattcaa caatttagcc aactaggtgc
    26101 gcaagtgcat ctggatgatt ttggtaccgg ctactcttca ctttcgcaac tggcgcgctt
    26161 tccgatcgat gccatcaaac ttgaccaggt ttttgttcga gatattcaca aacaacctgt
    26221 ctcgcagtca ctggtccggg cgatcgtcgc tgtggcccag gcattgaatc ttcaggtgat
    26281 cgccgaaggt gtagagagtg caaaggaaga tgctttttta accaagaacg ggatcaatga
    26341 gcggcaagga tttttgtttg ccaaaccgat gcccgccgtc gccttcgaac gctggtataa
    26401 acgctatctg aagcgcgcat aagtagatga atatatatac ttagcgctat atcccgtcat
    26461 tatatatcgt attcctggat gataattagc cgttgctgtt aaacacttct cttttagcgg
    26521 caatttttgc attattcatt ttcccccttt tgctataagg atgtccttga tgtctgaatt
    26581 tgatgcccag cgcgttgccg aacgtattga tattgtgtta gatatccttg tggcaggcga
    26641 ttaccactct gcgatccata atctcgagat acttaaagca gaactcttgc gccaggttgc
    26701 tgaatctacg ccagacatcc cgaagacacc gtgggagatt taacaatcgt gccttcaggt
    26761 tttcctattt gaggatgccc ggcgcgtcag tattttttta tttagtatta taacgttata
    26821 agaattacag cgatgaattc ccgacaacaa actattctac agatggtcat tgaccagggt
    26881 caggttagcg taaccgatct ggcaaaagcc actggagttt ctgaagttac cattcgccag
    26941 gatctcaaca ccctcgaaaa actgagttac ctccgccgtg cacatggctt tgcagtttcg
    27001 cttgatagtg atgacgtcga aacccgtatg atgagcaact atacgctgaa gcgtgaactc
    27061 gccgagtttg ccgcgtcact ggttcaaccg ggcgaaacca tctttatcga aaatggcagc
    27121 agcaatgccc tgctcgcccg gactctgggg gagcagaaga aaaatgtcac tatcatcacg
    27181 gtcagcagct acatcgcgca tttgctgaaa gacgcgcctt gtgaagtcat tttgctcggt
    27241 ggcgtgtacc agaaaaaaag cgaaagtatg gttggccctt tgacacgcca gtgcatccag
    27301 caggtacatt tcagcaaagc atttattggt attgatggct ggcaacctga aactggattc
    27361 accggtcgcg atatgatgcg caccgatgtg gtcaatgccg tgctggaaaa agagtgcgaa
    27421 gcgatagtcc tgactgacag ctcgaaattt ggtgctgtac attcatactc catcggtccc
    27481 gttgagcgat tcaatcgcgt gattaccgat tcgaaaatac gcgccagcga tctgatgcat
    27541 cttgagcaca gtaaactcac cgttcacgtc gttgacattt aaccatttcc cttctctacg
    27601 gatgatttgc agtttggcaa atcatccgct ctaagatgat tcctggttga taattaagac
    27661 tatttacctg ttattaacac tctcaagata taaaattatt atcagcgata taacaggaag
    27721 taattatcac ctgcgtgata taaccctgcg cgcgagcaga tttcacggaa taatttcacc
    27781 aggcttattc ttagctatta tagttataga gagcttactt ccgtgaatca taaattcagg
    27841 agagagtatt atgtttgtaa cgagcaaaaa aatgaccgcg gctgttctgg caattacttt
    27901 ggcaatgtct ctgagtgcct gttctaactg gtctaaacgg gaccgcaaca ccgcaatcgg
    27961 cgcgggtgca ggggcattag gcggtgcagt actgaccgat ggcagtacgt tgggtacatt
    28021 aggtggtgca gctgtcggtg gtgttattgg tcaccaggtc ggtaaataat cgtaaataaa
    28081 tgtcgattat cgactcgcaa ttataatacg cttgattctg ttcagtacga aataactgaa
    28141 taatacacac agtaaaatat tcgaaagcca cggatatatc cgtggctttt tatgttaacc
    28201 gcctgcgagt tttaccttca tccctttcgc ttcaagtagt gattttaata aatcacgctt
    28261 atcgccctgg atttcaataa ctccatcttt aactgctccg ccgcagccgc attttttctt
    28321 cagttccgct gcaagttttg tcagttcggc atcatcgaga tcgataccgg taatcaggca
    28381 aacgccctta cctttacgtc cactggtctg acgctgaata cgcaccgctc catcgccttt
    28441 agggcgtact ggggccgctt tgggctcatc aatacgcccg gtttccgttg agtagaccag
    28501 acggctgttg gaatcactca tcatgcactc cgctgtaaag aggcgttgat cgctttcagt
    28561 gtctgcgctg gatctaccga ttgcgttacc gggcgaccaa tcaccatata atcaacacca
    28621 gccgccaacg cctgttctgg cgtcataatg cggcgctggt caccagcttc actcccctgc
    28681 ggacgaatgc ccggtgtaac cagtttgaac tcctgaccga atacctgttt aaagcgcaca
    28741 gcttcctgag cagaacacac cacaccatca aggtcacatt tttgcgtcag tgctgccaga
    28801 cgttctgcat aatctgcagg tgacagtgtc acgccaagat cggccaggtc gctggcttcc
    28861 atgctggtca acactgtcac agcaatcaaa agcggtgcat ctttgccaaa cggaaccagt
    28921 gcctcacgcg ctgcggtcat catacgcgcc ccaccggagg catgaacatt caccatccac
    28981 acgcctaagt cagctgcagc agcgacagcg tgcgctgcag tgttggggat atcgtggaat
    29041 ttcaggtcaa gaaagatatc aaaaccacgc tgttgaagtt cgcgcacaaa ctgtggccca
    29101 aacaatgtaa acatctcttt accgaccttc agacgacaat cgcgtgggtc gatcttgtcg
    29161 acaaaggaca gcgcgtcatc acaattatga taatcaaggg caacaaccac aggagaattc
    29221 gtaacggcgc gggaagaaga tgaagcagtt aacgtcatga ccagaccttc ttgatgatgg
    29281 gcaccgggag tggcgcgaaa caggtaaacg gcgagcattc tacccgtgaa aataaaaaat
    29341 taacagttgc gatttttccc ctagcaggct ttttctgctc cgtcgttgcc ggagcacaat
    29401 gggacatcaa taactagtat gttgtaacta aagtcggatt tttttaaaaa ttacaggcca
    29461 tcaagaccgc gaatcggttt aatagttgac caggcacgac aagacggaca atgccagtag
    29521 agggtgtatg cggtaaaacc acatttctgg cagctataac gaggcttact acgcaccttc
    29581 tcgccaacca tgtcacgcag caccatcaga ctctctttgg cgcgcccttc ttccgcttca
    29641 tttaagtggt aatccattag cttatggaat acacgcatgg tcggatgacg ctgaagctgg
    29701 cgtgtaatat agacctgtgc agcctcacta ccgtcgcgcg cttcgatgat atcagcaagc
    29761 atcaattcag catcggcacc ggtgttctct tccaccgcgc gttgcaggaa ttctgcccat
    29821 tcggcagttt tacccaactg ctgatagcag gtttgcaaca tttccagcgt ttcgctgacc
    29881 agttctctgt cctgggatat gacgcgttgc aaactttcga cggctttggc gtattctcct
    29941 ttcgccataa acacgcgtcc catcatgatg gatacgcggg cgctgttttt atctgccgcc
    30001 gccccttttt ttagcaaggt catggcacga tcgagatcgt cgctggccat atgctgcagg
    30061 gctaactcac agtagaaatg ggcaatttcg acgcgctgtt tatctttacc cagcttcacc
    30121 aggcgttcgg caacatcaat tgctttctgc cactcactgg tagcctggta gatttgtagc
    30181 aactgttgca gcgcgccaat gcggaagtca gtttcatcgg tcagctgatt gaacatgtct
    30241 tccgcgcggt cgtataaccc ggcggccatg taatcacgcc ccagttgttg aatcgccaac
    30301 agacgctgtt cataggtcag cgaggcgctt tccattaggg tctgatggat gcgaatagcg
    30361 cgatcaactt cgccacgcga acggaacagg tttccgagcg taaggtgggc ttcaacggtg
    30421 cctgtatcct ctttaagcat atcgagaaac aggtctaccg ctttatcctg ttgattacta
    30481 agcaggaagt taacccccgc tacgtaatca cgcgacaagc ggttggcttc atcttgcttg
    30541 ttttgttgcg cacttctgcg gcccatatac cagccatagg cagcggctac aggcaaaagc
    30601 agaaacaaca actccagcat agaaagttat tccttcgccg ctgacgagtg cggcactaca
    30661 gccacgtcag tcgcgggtga aagctggttt tccagtcgct ttattttacg ttcagcgcgc
    30721 gccagggaaa cacgaactcg cagccagaac aggccacaaa tcaaccaacc gatagcaaac
    30781 cccgcagcaa acaataccgc cagcaatgtg gaaatacggt actccccttg cgctaacaga
    30841 taattaaacg tcacctgttg atcgttctgc gcacccaacg tgaccgaaat cacgaagatc
    30901 gctaacacca gtaagaaaat gagtaaatat ttcacattac ttcccgttat gtggttagag
    30961 cgaataaatt atggtcaact taccgcaata gccctttaaa gtaccatttt cgcgccgtct
    31021 gcgaaatggc atccacgcgc attgttgatg atttaaggcc taaattcgga aaatcaacca
    31081 gcgttaactt tcttgttctc gttgtgcaat ttcgcgattt tcttccgcag gtggtgttaa
    31141 tggcccacaa attcgttgcg caagccacgt tgccaccgcc accagcgccc acgaaatcaa
    31201 cgtagctacc accagatcgc gtggccaatg catcccgagc agcaggcggc ttcccatgac
    31261 tcccgttgcc cagaccaaca agatagcaat ggttaacgtt cgccgacgcg gccacagcaa
    31321 accaacggcc agcagtgccc aactggcagc aaacatcgtg tgaccggaag gaaaggcaaa
    31381 ccccgtctct ttctgccagt gtgaacgcaa atattgtgga atatttttct cttcagccaa
    31441 ctgttctttt actagatttc cgcgttctgc tcgctttaaa gtgtagaact catcaaccgg
    31501 aatatgatgt gttttttcca accagataac aaaaggtcgt ggttcctgta ctttgtcttt
    31561 gatccaggat ttaacgcctt gtccaacaag gattgccgcc gccagaatgg caaataatac
    31621 aaaggcagcc ttaatgcgaa aacgcagaca ccagagaaac cagccgaaca aaatcaaatg
    31681 tgtaatgacg ccccagggct gggtgacagt ttcagtaacc caaaaagccg cttttagtag
    31741 ccaactttgt tctccaggtt gccaacgcca gccagaaatc catacggcta ctggcatgac
    31801 aagcaatagt gcagctccca ctgcggtacg tcgggcaatc gaacgcatgg cctctccttt
    31861 ttgataagtc ccacaatcat aactgaaaat gccagttcca agaaaaattg acagatttgt
    31921 gccattccgt gaacgatcga cgcgtcgtga ttaggtgaac cccttctcgt tatggcaaaa
    31981 taagccaata cagaaccagc attatctgga gaatttcatg cagcttaaac gtgtggcaga
    32041 agccaaactg ccaaccccat ggggcgattt cctgatggtg ggatttgaag aactggcaac
    32101 cggacacgat catgttgcgc tagtctatgg cgatatttcc gggcataccc cggtacttgc
    32161 gcgcgtccat tccgaatgtc tgaccggtga cgccctgttc agcttgcgct gcgattgtgg
    32221 cttccagctc gaagcggcat tgacgcaaat tgccgaggaa ggccgtggta ttttgctgta
    32281 tcaccgtcag gaaggtcgta acattggtct gctgaataaa atccgcgctt acgcactgca
    32341 ggatcaaggt tacgataccg tagaggctaa ccaccagtta ggcttcgccg ctgatgagcg
    32401 cgacttcact ctttgcgctg atatgttcaa actccttggc gtcaatgaag tccgcttgtt
    32461 aaccaataac ccgaaaaaag tcgaaattct gaccgaagca gggattaata ttgttgaacg
    32521 cgtaccattg attgtaggtc gtaaccccaa taacgaacat tatctcgata ccaaagccga
    32581 gaaaatgggc catttgctga acaaataacc ctcttgcatc gtgtaattca tttgcttgcc
    32641 ggaagcaaaa taaccggcaa gcaaatactt gttacttcaa catattacga atgacataat
    32701 gcaaaatgcc gtcgttctgg tagtaggtca actccgtcgc ggtgtcgata cgacaacggc
    32761 agggtacgac ttcctggcta ccatccgcgc gcgtaagcgt caccggaacc gtcgcgccag
    32821 gttgtagatt ttgcagatcg ccaatatcaa tcttctcttc cccggttagc cctaacgttt
    32881 tacgcgtcac gccttgcgga aattccagcg gcaggatgcc catgccaatt aaattcgaac
    32941 ggtgaattcg ttcaaacgat tcggcaatca ccacacgaat tccaagcaga cgaggtcctt
    33001 ttgccgccca gtcacggctt gagcctgatc catactcttt cccggcaatc accgccagcg
    33061 gcgtttgctc ctgcttatag cgcatcgcag catcataaat agagactacg tcgctgtcag
    33121 gtaaatgccg cgtcatcccc ccttcaacgc caggcaccat ttcattacgg atgcgaatat
    33181 tggcgaaggt gccgcgcatc atcacttcat ggttaccacg ccgcgaaccg taggagttaa
    33241 agtcttttcg ctcaacaccc cgaccttgta gatatcgacc cgctgggctg tcgggcttaa
    33301 tactgcccgc cggagagata tggtcagtgg tgactgaatc ccccagcatt gcgaggatcc
    33361 gcgcaccgtg aatatcttcc actggtgctg gtgttgcctg catttcatca aagaaaggcg
    33421 ataagcgaat ataggttgag tcctcctgcc aaccgtaggt atcggatcgt gtgacgttaa
    33481 tttccttcca ctctgctgtg ccttcaaaaa cttctgcgta ctctttgcgg aacatttctg
    33541 tggagacttg ttctaccgca cgggcaattt cttgtgccga tggccagata tctttcagat
    33601 aaaccggctc gcctttgcga tcatggccga taggctcaga agccaggttg atattcatat
    33661 ttcccgccag cgcataggca accaccagcg gtggcgaggc cagccagtta gttttaacca
    33721 gcggatggat acggccttca aagttacggt tgccggacag caccgcaccg acggttaaat
    33781 cgcctttttt gattgccgtt tcgataggat cgggcagcgg cccagagtta ccaatacagg
    33841 tggtacaacc gtatcccaca aggttaaacc ccagttcgtc gagatacggt gtcagtttcg
    33901 cttttgccag ataatcagaa acgactttcg aacccggtgc caacgacgct ttgacccacg
    33961 gttgccgctt gaggccaaga gttacggctt ttttcgccag caagcctgcg gccatcagca
    34021 cacttgggtt agaggtgttg gtgcacgagg ttatcgcagc aatgaccaca gcgccatcag
    34081 gtaactgata ctgatgtccg ttcataacat aatcgactgg caggcgatct ttatgcgtgg
    34141 cattcacttt cagttcgtta ctggcggcaa atgcttttgg tacatcgggc agtgcaacgc
    34201 gatcctgtgg gcgttttggc cctgccaggc tcgcttcaac gtcattcata tccagttcta
    34261 acatactggt aaaaatcggt tcatcgcccg ggttacgcca catgccctgc gctttggcat
    34321 atttttcgac caactcgacc tgatcttcgc tgcgcccgct taaacgcatg taatcgaggg
    34381 ttactgcatc gattgggaag aagccacagg tggcaccata ttctggcgac atattggcaa
    34441 tggtggcgcg atccgccaac ggtagtgaat ccagaccatc accataaaat tcgacgaatt
    34501 tccccaccac gccatgtttg cgcagcattt gggtaacagt gagaaccagg tctgtggcgg
    34561 taataccttc acgtaatttt ccggtaagtt taaagcccac tacgtccggg ataagcatgg
    34621 aaaccggctg gcctaacatg gcggcttctg cttcgatccc acccacgccc caccccagca
    34681 cgccaaggcc gttgatcata gtggtgtgcg agtcagtacc aacgagtgta tccggataag
    34741 caatccattc accgtcctgc aattcgctcc acactgcttt gccgagatat tcgaggttaa
    34801 cctgatggca aatgcctgtg cctggcggca cgacgctaaa ccgactgaac gcttgctttc
    34861 cccatttcag gaacacataa cgttcgtggt tgcgctccat ttccaggcgt acgttttctt
    34921 caaatgcctc atcatcacca aaacgatcga cggtcaccga gtggtcaatg accaggtcga
    34981 ccggtgagag cgggttaacc tttgcagtat cgccaccgag gcgtttaacc gcttcgcgca
    35041 ttgccgccag atcaacaacg gcaggtacgc cggtaaagtc ctgcatcagc acccttgccg
    35101 ggcggtaggc aatttcacgg tcagcatggg catttttcag ccatcccgcc agtgcgtgga
    35161 tatcctcttc ggtaactgag ttaccatcct gccagcgcag caggttttcg agcaaaactt
    35221 tgagtgactt gggtagacgg gtgatatcgc ccagtgattt agcagcaagc ggcaggctgt
    35281 agtagtggta agttttatct ttggcctgca acgtgtcctt actggcttct cgtagggttg
    35341 acgacatagc tcctccttaa tgacagggtt gcgaagccct gattctcttc agggtgaata
    35401 ttaaagataa cacaaacatc gcgtaacgat ttgataacaa cccaaattga taaaagagga
    35461 gaaaccttgt ggcaaagcaa atgacaaccc cgccgcagcg gggtcaagga tcagtgagta
    35521 atgatccaga ggagttcggc ccagaagagc atcgacagag aaaacgcgcc catccaggac
    35581 caatatttca tatttgtgtt gatcataggc tacaccctta acggttatta ttttgtgttg
    35641 agataactca agctgttatt gctgcggtgt attaaaagcc ctttaattca ttaaaaatgg
    35701 gtaataagaa tgatgataat tattgtttcg gctgatcatc ttcagcaaac agttctataa
    35761 aggcttgctg ctgcgatgtt agttgccagt cgtctggaat aacgacggaa cattcccgtt
    35821 tcgtttctgg ttcattgtca ctatgctggc ataatggtga cgactccagt tgctcctgac
    35881 cgaccatagt tatccccaca ctttccaaat cagcagtgcg acaaccaccc agaaaagtgc
    35941 agaaccaaga aaaaccgcca gccaggcttt acgttttaat tcaggatcac ggcggggttc
    36001 ctgatttcca gaaggcattg ctaacctcat ataatcgcca tcacttatca gcaagacgtt
    36061 gaatgattgg tgtaattaat catacgatga gacaaatcct aaagaaactt tagtcaaaaa
    36121 tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata
    36181 tttgaaaact ggaatttgcg aggcgggtaa ttagacacta ctttacaggt aatttaccct
    36241 ataaatatga gggataaaaa tattgtgcac ggtatagaaa taagtaacaa aagagaatat
    36301 attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt atttttccgg
    36361 cagttttata tctttaaaca tgacctcaat ttcttcattg gagcgcaatg cgacagccgc
    36421 atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat acatataact
    36481 gcgcaaaaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga agatatcgtg
    36541 agcatcaacg cgcacaaggt cgggatcggc gaccggatcc accgccatgc tggcaatgac
    36601 ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg ttgccgtgaa
    36661 aacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta tccagttctg aacgtccggt
    36721 aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg taatggcttt
    36781 tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt ggtagcacgg
    36841 taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagcga aatcggcatt
    36901 gcctttagag acggcatcag caatttgtgt cggcgggccc tggtgcatat gcaaagaaac
    36961 gcggggataa cgctcaataa agcctttgat aacgtttggt aacgcgtagc gtgcctgggt
    37021 atgcgtggtg gcgatataca gcgagccttt atccggccag gtgtgctcac cggcaaccga
    37081 ttttatggca tcgactttcg acaggacttc gcgagcgata cgaattattt cttgtcctgc
    37141 tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc ctagctcgtc
    37201 ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc cttccgctgt
    37261 tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt gttgtaattt
    37321 catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac acctggcggg
    37381 tgactaatac gatttaataa taaaaaaggt gctaactata accactatat catttatagc
    37441 cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc tttcgcgacc
    37501 ctttgtttat aaaaacctga cagaattaaa ggttattttt ttccttcaac ccatttgcca
    37561 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
    37621 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgtggc
    37681 gcatcggcca gataacgcag tttttccggc agacggtcgc ggaagcgata aagctcttcc
    37741 accagtggcg cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaacacaccg
    37801 gcagcaccgt cacgcagcac gaaataagca tctgattttt cgcacggcag ctcaggtaat
    37861 ggcaccggat cttctttcgg tggtgccact tcaccgttac gtaaaatctt acgtgtgttt
    37921 ttacactctt cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt
    37981 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg
    38041 ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt
    38101 ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg
    38161 cgcagcgcgt tggtttcagc atcttcgcct tccagcacgt tcagcacttc gttttccggc
    38221 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcatagcc agaacagcca
    38281 aggaataccc cggtactcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca
    38341 atgctggtca gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct
    38401 ttatctaact gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
    38461 tcgtgatttg ccacctggtc gaggctgttt tccatctgcg cggtaaagtc gtagttcatt
    38521 aactcgcgga aattttcttc aaggcgatcg gtgacgattt cgcccatttt ttccgcatag
    38581 aaacgacgat tttctactcg cacatagcca cgatcctgaa tggtcgaaat gatcgacgca
    38641 taggtagacg gacgaccgat accacgtttt tccagttctt taaccagcga tgcttcactg
    38701 aaacgggctg gcggcttggt aaagtgctgg gctggtgtta gttcaacgag cgtcagagca
    38761 tcgcctttat cgactgccgg taagatacga tcttcatcgc ctttacgcag tgcaggcatc
    38821 acttttgtcc agccatcaaa gcgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
    38881 gcaccaacgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggcaacgaac
    38941 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc
    39001 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
    39061 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
    39121 ccgcgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg
    39181 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaaacca
    39241 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
    39301 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
    39361 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcattctg atgagtcacc
    39421 tgcaacgcca acgcttcacc agatggcgta gtcgtgctgg catcgacttc ccagaactct
    39481 tccggtacga acgctttaat ttcacgctca cgctctacca ccaggcggac cgccaccgac
    39541 tgcacacgac cggcagacag accacgagcg atctttttcc acagcagcgg cgaaaccata
    39601 taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
    39661 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
    39721 actcggctat agcgcgcatc atcaccacca atcacttccc gcaggtgcca tgcaatggct
    39781 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
    39841 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
    39901 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
    39961 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc
    40021 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cacaacccag atacttgttg
    40081 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
    40141 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
    40201 taccgatatg gactcgctct ctgcggatat caaccccttg tcgtgactct gttcacgaaa
    40261 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggaattttg
    40321 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
    40381 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
    40441 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
    40501 gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt gaaagaggcc
    40561 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
    40621 cagcgtaatg gcgtgctggt atttaccggc gactactttt tagatgaaca aggattgcca
    40681 acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
    40741 aaatatcatc tggttgatta aagcaaaacg cgaggtctta gcctcgcgtt tgtcttttac
    40801 atcaatggct tttgaccccg ctgccaccag cgtagcaaca atcgatcggc gctctctgcc
    40861 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attaaccact
    40921 tcacggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
    40981 cctttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
    41041 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcagctct
    41101 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag cgtgcgctta
    41161 tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
    41221 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
    41281 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
    41341 gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca
    41401 tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct
    41461 gcgagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
    41521 ttaaaatcca gcacccagac gcgaggttta ctgtcagttg ccacctcgcc cagtttggct
    41581 ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt
    41641 ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
    41701 tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg
    41761 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt agccaaaaac
    41821 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
    41881 acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga
    41941 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
    42001 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
    42061 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
    42121 gagccagcga tgggattggt cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag
    42181 tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
    42241 aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaaaatt
    42301 gccaacaact ggcacagcgc attgtcgtta attatccgcg tctggatggt gttttgcata
    42361 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca aaatccgcag gtctggcagg
    42421 acgtcatgca gatcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat
    42481 tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttgga cgtcagggac
    42541 gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
    42601 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
    42661 ccgcaatgcg cgcaagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg
    42721 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
    42781 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
    42841 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
    42901 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
    42961 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
    43021 cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt ttcaggtgat
    43081 ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg cctgccgcga
    43141 agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg ttttgcttga
    43201 tgaactgacg tatatggtgg cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt
    43261 aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc atcgggatat
    43321 tcttgaactg gcggatacgg taagtgagct gcgccccatc agacatgcgt ttgatgccgg
    43381 tgttaaggcg caaataggga ttgattatta atctgtaaaa atgccggata tgacgtccgg
    43441 catactttga ttaaccgtta ttacgcccgc cagaacgacg actgccgctc acctgactgt
    43501 gacgtttcac cgcacgacga atctgattag ccttcatgcg acgacggtct ttttctacag
    43561 cgactttaga gctggtttct ggcggaagct ccaccagttc gcgcagatag ttagtctggg
    43621 cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt tggcagtggg atatcaccgt
    43681 aacgaacacg gatcaggcgg ctcacctgca caccaaccgc ttcccacaga cgacgaactt
    43741 cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct tcgccgccgc
    43801 tgaacttgat ggttttaaaa gctgccggac catcttccag ttgcacgcca cgactcaaat
    43861 cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcgtattca cgttcaactt
    43921 cacggcttgg atgcattaaa cggtttgcca gttcaccatc ggtggtgaac agcagcagac
    43981 cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcgccacgc agctttggca
    44041 gacggtcaaa caccgtcgga cgcccttccg gatcgttacg ggtacacaac tcaccttccg
    44101 gtttgtaata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc gaaatcagat
    44161 gaccatcgat acgaattttc agcccagggg taacttcaac gcgatcgccg agtttagcaa
    44221 ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca cggcgggagc
    44281 cgtggccagc gcgggccagc actttctgta atttttcgct catagagctt ccttttggtg
    44341 tcgccttcac aggcgtcgaa caggagaatc aatggcgccg tatagcgcca ttttaaggcc
    44401 gcgtagtata cagagttacg cacttataag aaaggcttca catcacctac gccttcacgc
    44461 accacgaccg gcgtatcatc ggtaagatca ataaccgttg ttggtttctg cccgagatag
    44521 ccgccgtgaa taatcaaatc tacctgtttt tccagacggt ctttaatttc ttcgggatcg
    44581 gattcggtaa attcgctgcc tggcagcatt agcgaagtgg aaagcatcgg ttcgcccagt
    44641 gcttcaagca acgcctgcgc aatcgggtta gaaggcacac gcataccgat ggttttgcgt
    44701 ttctcctgca acagacggcg tggcacttcc ttcgtcccct tcaggatgaa ggtatagttg
    44761 cccggcgtgt tgtttttcat caaacgaaac gcaacgttat ccacgaatga ataggtcgag
    44821 agttcagaaa gatcgcgaca catcagggta aagttgtgac cgtccggcag ctggcgaata
    44881 cgacaaatac gctccatggc gtttttatct tcaattttac agccgagcgc atagccggaa
    44941 tcagttggat agacaatcac cccgccttta cgcacgatct ccaccgcctg gttgatcaga
    45001 cgttgctgcg ggttatcagg atgaatataa aaaaactggc tcataattcc ctctctgtgg
    45061 tgttctgcgg ctgttcccat agctgccaga cgccttcaac gcctgcgggc agccagagtt
    45121 tacgacccag ttcgatccac gggcatggct gatgaaaatc agatccttgt gatgcccata
    45181 aatgatgctg acgcgcaagt gtggccagct gggtgcgttc attgggcgat tgctggcact
    45241 gcgcgacttc catcgcgtcg ccgtggtgtt cggcaaaatg cgcaaccaat cttttcagcc
    45301 atttagcact aagattgtac cgcccaggat gagctagcac cgccttaccg ccagaatgat
    45361 gaatgacatc aatagcttgt tctattgtac accactgtgg cggaacgtat ccggttttcc
    45421 cgcgcgccag atacttttta aagacatctg ccattgaact ggctttgccg cactccacaa
    45481 ggaaacgcgc aaaatgaccg cgcgtcactg cgccgccctg cgccagtcgt tgtgcgcctt
    45541 ccagcgcccc aggtatttgc gctttttcaa gtctttcggc aatttgctgc gcccgctgat
    45601 tgcgccgttc tgtctgctgc gcgaggaact cacacattag cggatgagta atatcaatat
    45661 tcagcccgac aatatgaatt tcatgatttt cccagaccgt ggaaatttcc acgccgggaa
    45721 taagattcag cgccagtccc gaacgtgaaa tttcctctct tgctggcgcg attgcagctg
    45781 tggtgtcatg gtcggtgatc gccagggtgc cgacgcgcat ctcgactgca cggtgcacca
    45841 atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca
    45901 ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac
    45961 atcataacgg gtctggcaaa tattctgaaa tgagctgttg acaattaatc atcgaaccag
    46021 ttaactagta cgcaagttca cgtaaaaagg gtatcgacaa tgaaagcaat tttcgtactg
    46081 aaaggttggt ggcgcacttc ctgaaacggg cagtgtattc accatgcgta aagcaatcag
    46141 atacccagcc cgcctaatga gcgggctttt ttttgaacaa aattagagaa taacaatgca
    46201 aacacaaaaa ccgactctcg aactgctaac ctgcgaaggc gcttatcgcg acaacccgac
    46261 tgcgcttttt caccagttgt gtgggaatcg tccggcaacg ctgctgctgg aatccgcaga
    46321 tatcgacagc aaagatgatt taaaaagcct gctgctgata gacagtgcgc tgcgcattac
    46381 agcattaggt gacaccgtca caattcaggc actttccggc aatggcgaag ccctgctggc
    46441 actactggat aacgccctgc ctgcgggtgt ggaaagtgaa caatcaccaa actgccgtgt
    46501 gctgcgcttc ccccctgtca gtccactgct ggatgaagac gcccgcttat gctccctttc
    46561 ggtttttgac gctttccgtt tattgcagaa tctgttgaat gtaccgaagg aagaacgaga
    46621 agcaatgttc ttcggcggcc tgttctctta tgaccttgtg gcgggatttg aagatttacc
    46681 gcaactgtca gcggaaaata actgccctga tttctgtttt tatctcgctg aaacgctgat
    46741 ggtgattgac catcagaaaa aaagcacccg tattcaggcc agcctgtttg ctccgaatga
    46801 agaagaaaaa caacgtctca ctgctcgcct gaacgaacta cgtcagcaac tgaccgaagc
    46861 cgcgccgccg ctgccagtgg tttccgtgcc gcatatgcgt tgtgaatgta atcagagcga
    46921 tgaagagttc ggtggcgtag tgcgtttgtt gcaaaaagcg attcgcgctg gagaaatttt
    46981 ccaggtggtg ccatctcgcc gtttctctct gccctgcccg tcaccgctgg cggcctatta
    47041 cgtgctgaaa aagagtaatc ccagcccgta catgtttttt atgcaggata atgatttcac
    47101 cctatttggc gcgtcgccgg aaagctcgct caagtatgat gccaccagcc gccagattga
    47161 gatctacccg attgccggaa cacgcccacg cggtcgtcgc gccgatggtt cactggacag
    47221 agatctcgac agccgtattg aactggaaat gcgtactgac cataaagagc tgtctgaaca
    47281 tctgatgctg gttgatctcg cccgtaatga tctggcacgc atttgcaccc ctggcagccg
    47341 ctacatcgcc gatctcacca aagttgaccg ttattcctat gtgatgcacc tcgtctctcg
    47401 cgtagtcggc gaactgcgtc acgatcttga cgccctgcac gcttatcgcg cctgtatgaa
    47461 tatggggacg ttaagcggtg cgccgaaagt acgcgctatg cagttaattg ccgaggcgga
    47521 aggtcgtcgc cgcggcagct acggcggcgc ggtaggttat ttcaccgcgc atggcgatct
    47581 cgacacctgc attgtgatcc gctcggcgct ggtggaaaac ggtatcgcca ccgtgcaagc
    47641 gggtgctggt gtagtccttg attctgttcc gcagtcggaa gccgacgaaa cccgtaacaa
    47701 agcccgcgct gtactgcgcg ctattgccac cgcgcatcat gcacaggaga ctttctgatg
    47761 gctgacattc tgctgctcga taatatcgac tcttttacgt acaacctggc agatcagttg
    47821 cgcagcaatg gtcataacgt ggtgatttac cgcaaccata ttccggcgca gaccttaatt
    47881 gaacgcctgg cgacgatgag caatccggta ctgatgcttt ctcctggccc cggtgtgccg
    47941 agcgaagccg gttgtatgcc ggaactcctc acccgcttgc gtggcaagct gcccattatt
    48001 ggcatttgcc tcggacatca ggcaattgtc gaagcttacg ggggctatgt cggtcaggcg
    48061 ggcgaaattc ttcacggtaa agcgtcgagc attgaacatg acggtcaggc gatgtttgcc
    48121 ggattaacaa acccgctgcc ggtggcgcgt tatcactcgc tggttggcag taacattccg
    48181 gccggtttaa ccatcaacgc ccattttaat ggcatggtga tggcagtacg tcacgatgcg
    48241 gatcgcgttt gtggattcca gttccatccg gaatccattc tcaccaccca gggcgctcgc
    48301 ctgctggaac aaacgctggc ctgggcgcag cagaaactag agccagccaa cacgctgcaa
    48361 ccgattctgg aaaaactgta tcaggcgcag acgcttagcc aacaagaaag ccaccagctg
    48421 ttttcagcgg tggtgcgtgg cgagctgaag ccggaacaac tggcggcggc gctggtgagc
    48481 atgaaaattc gcggtgagca cccgaacgag atcgccgggg cagcaaccgc gctactggaa
    48541 aacgcagcgc cgttcccgcg cccggattat ctgtttgctg atatcgtcgg tactggcggt
    48601 gacggcagca acagtatcaa tatttctacc gccagtgcgt ttgtcgccgc ggcctgtggg
    48661 ctgaaagtgg cgaaacacgg taaccgcagc gtctccagta aatccggctc gtcggatctg
    48721 ctggcggcat tcggtattaa tcttgatatg aacgccaata aatcgcgcca ggcgctggac
    48781 gagttaggcg tatgtttcct ctttgcgccg aaatatcaca ccggattccg ccacgcgatg
    48841 ccggttcgcc agcaactgaa aacccgcacc ctgttcaatg tgctggggcc attgattaac
    48901 ccggcgcatc cgccgctggc gttaattggt gtttatagtc cggaactggt gctgccgatt
    48961 gccgaaacct tgcgcgtgct ggggtatcaa cgcgcggcgg tggtgcacag cggcgggatg
    49021 gatgaagttt cattacacgc gccgacaatc gttgccgagc tgcatgacgg caaaattaag
    49081 agctatcaat tgaccgctga agattttggc ctgactccct accaccagga gcaactggca
    49141 ggcggaacac ctgaagaaaa ccgtgacatt ttaacacgct tgttacaagg taaaggcgac
    49201 gccgcccatg aagcagccgt cgctgcgaac gtcgccatgt taatgcgcct gcatggccat
    49261 gaagatctgc aagccaatgc gcaaaccgtt cttgaggtac tgcgcagtgg ttccgcttac
    49321 gacagagtta ccgcgctggc ggcacgaggg taaatgatgc aaaccgtttt agcgaaaatc
    49381 gtcgcagaca aggcgatttg ggtagaaacc cgcaaacagc agcaaccgct ggccagtttt
    49441 cagaatgagg ttcagccgag cacacgacat ttttatgatg cgctacaggg tgcacgcacg
    49501 gcgtttattc tggagtgtaa aaaagcgtcg ccgtcaaaag gcgtgatccg tgatgatttc
    49561 gatccggcac gcattgccgc catttataaa cattacgctt cggcaatttc ggtgctgact
    49621 gatgagaaat attttcaggg gagctttgat ttcctcctca tcgtcagcca aatcgccccg
    49681 cagccgattt tatgtaaaga cttcattatc gacccttacc agatctatct ggcgcgctat
    49741 taccaggccg atgcctgctt attaatgctt tcagtactgg atgacgacca atatcgccag
    49801 cttgcagccg tcgctcacag tctggagatg ggggtgctga ccgaagtcag taatgaagag
    49861 gaactggagc gcgccattgc attaggggca aaggtcgttg gcatcaacaa ccgcgatctg
    49921 cgtgatttgt cgattgatct caaccgtacc cgcgagcttg cgccgaaact ggggcacaac
    49981 gtaacggtaa tcagcgaatc cggcatcaat acttacgctc aggtgcgcga gttaagccac
    50041 ttcgctaacg gttttctgat tggttcggcg ttgatggccc atgacgattt gcacgccgcc
    50101 gtgcgccggg tgttgctggg tgagaataaa gtatgtggcc tgacgcgtgg gcaagatgct
    50161 aaagcagctt atgacgcggg cgcgatttac ggtgggttga tttttgttgc gacatcaccg
    50221 cgttgcgtca acgttgaaca ggcgcaggaa gtgatggctg cggcaccgtt gcagtatgtt
    50281 ggcgtgttcc gcaatcacga tattgccgat gtggtggaca aagctaaggt gttatcgctg
    50341 gcggcagtgc aactgcatgg caatgaagat cagctgtata tcgatacgct gcgtgaagct
    50401 ctgccagcac atgttgccat ctggaaagca ttaagcgtcg gtgaaaccct gcccgcccgc
    50461 gagtttcagc acgttgataa atatgtttta gacaacggcc agggtggaag cgggcaacgt
    50521 tttgactggt cactattaaa tggtcaatcg cttggcaacg ttctgctggc ggggggctta
    50581 ggcgcagata actgcgtgga agcggcacaa accggctgcg ccggacttga ttttaattct
    50641 gctgtagagt cgcaaccggg catcaaagac gcacgtcttt tggcctcggt tttccagacg
    50701 ctgcgcgcat attaaggaaa ggaacaatga caacattact taacccctat tttggtgagt
    50761 ttggcggcat gtacgtgcca caaatcctga tgcctgctct gcgccagctg gaagaagctt
    50821 ttgtcagcgc gcaaaaagat cctgaatttc aagcccagtt caacgacctg ctgaaaaact
    50881 atgccgggcg tccaaccgca ctgaccaaat gccagaacat tacagccggg acgaacacca
    50941 cgctgtatct caagcgtgaa gatttgctgc acggcggcgc acataaaacc aaccaggtgc
    51001 tggggcaggc gttgctggcg aagcggatgg gtaaaaccga aatcatcgcc gaaaccgggg
    51061 ccggtcagca tggcgtggcg tcggcccttg ccagcgccct gctcggcctg aaatgccgta
    51121 tttatatggg tgccaaagac gttgaacgcc agtcgcctaa cgtttttcgt atgcgcttaa
    51181 tgggtgcgga agtgatcccg gtacatagcg gttccgccac gctgaaagat gcctgtaatg
    51241 aggcgctacg cgactggtcc ggcagttatg aaaccgcgca ctatatgctg ggcaccgcgg
    51301 ctggcccgca tccttatccg accattgtcc gtgagtttca gcggatgatt ggcgaagaaa
    51361 ccaaagcgca gattctggaa agagaaggtc gcctgccgga tgccgttatc gcctgtgttg
    51421 gcggcggttc gaatgccatc ggcatgtttg ctgatttcat caatgaaacc aacgtcggcc
    51481 tgattggtgt ggagcctggt ggtcacggta tcgaaactgg cgagcacggc gcaccgctaa
    51541 aacatggtcg cgtgggcatt tatttcggta tgaaagcgcc gatgatgcaa accgaagacg
    51601 ggcagattga agaatcttac tccatctccg ccggactgga tttcccgtct gtcggtccac
    51661 aacacgcgta tcttaacagc actggacgcg ctgattacgt gtctattacc gatgatgaag
    51721 cccttgaagc cttcaaaacg ctgtgcctgc acgaagggat catcccggcg ctggaatcct
    51781 cccacgccct ggcccatgcg ctgaaaatga tgcgcgaaaa cccggataaa gagcagctac
    51841 tggtggttaa cctttccggt cgcggcgata aagacatctt caccgttcac gatattttga
    51901 aagcacgagg ggaaatctga tggaacgcta cgaatctctg tttgcccagt tgaaggagcg
    51961 caaagaaggc gcattcgttc ctttcgtcac gctcggtgat ccgggcattg agcagtcatt
    52021 gaaaattatc gatacgctaa ttgaagccgg tgctgacgcg ctggagttag gtatcccctt
    52081 ctccgaccca ctggcggatg gcccgaccat tcaaaacgcc actctgcgcg cctttgcggc
    52141 aggtgtgact ccggcacaat gttttgaaat gctggcactg attcgccaga aacacccgac
    52201 cattcccatt ggcctgttga tgtatgccaa tctggtgttt aacaaaggca ttgatgagtt
    52261 ttatgcccag tgcgagaaag tcggcgtcga ttcggtgctg gttgccgatg tgccagttga
    52321 agagtccgcg cccttccgcc aggccgcgtt gcgccataat gtcgcaccta tcttcatctg
    52381 cccgccaaat gccgatgacg acctgctgcg ccagatagcc tcttacggtc gtggttacac
    52441 atatttgctg tcacgagcgg gcgtgaccgg cgcagaaaac cgcgccgcgt tacccctcaa
    52501 tcatctggtt gcgaagctga aagagtacaa cgctgcacct ccattgcagg ggtttggtat
    52561 ttccgccccg gatcaggtaa aagcagcgat tgatgcagga gctgcgggcg cgatttctgg
    52621 ttcggccatt gttaaaatca tcgagcaaca tattaatgag ccagagaaaa tgctggcggc
    52681 actgaaagtt tttgtacaac cgatgaaagc ggcgacgcgc agttaatccc acagccgcca
    52741 gttccgctgg cggcatttta actttcttta atgaagccgg aaaaatccta aattcattta
    52801 atatttatct ttttaccgtt tcgcttaccc cggtcgaacg tcaacttacg tcatttttcc
    52861 gcccaacagt aatataatca aacaaattaa tcccgcaaca taacaccagt aaaatcaata
    52921 attttctcta agtcacatat tcctcaggta attgttaata tatccagaat gttcctcaaa
    52981 atatatttcc ctctatcttc tcgttgcgct taatttgact aattctcatt agtgactaat
    53041 tttaatgagt gtcgacacac aacactcata ttaatgaaac aatgcaacgc aacgggagaa
    53101 ataacatggc cgaacatcgt ggtagttcag gaaatttcgc cgaagaccgt gagaaggcat
    53161 ccgacgcagg ccgtaaaggc ggtcagcata gcggcggtaa ttttaaaaat gatccgcaac
    53221 gcgcatctga agcgggtaaa aaaggcggtc aacaaagcgg tggtaataaa tcaggcaaat
    53281 cctgattcac cgttaattat taatatcagc tgataaagat aattgcatct gcgggccgta
    53341 tggctcgcag tgatttcact ggagaaaaat atgaatatga agaccattga agatgtattt
    53401 attcaactgc tttcagatac ctacagcgca gaaaaacaat taacccgggc actggcaaaa
    53461 ctcgcaagag caacatcaaa tgaaaaatta agtcaggctt ttcatgcgca cctcgaggaa
    53521 actcatggac agattgaacg aattgatcaa gttgtggaat cagaatcaaa tctgaaaatt
    53581 aagcgcatga aatgtgtggc aatggaaggt cttattgaag aagctaatga ggtcatcgaa
    53641 agtaccgaga aaaacgaagt gcgtgatgcc gcactgattg ccgcagcaca gaaagtcgag
    53701 cattatgaga ttgccagtta cgggacatta gcgacgctgg ctgaacaatt aggttaccgt
    53761 aaagcagcga agcttctgaa agaaaccctg gaagaagaaa aggccaccga catcaaactg
    53821 actgatctgg cccttaataa cgtaaataag aaagccgaaa ataaagcctg aaatatgaat
    53881 tttaactttt agtcatttta taaagaggac attttcatga atcgtattga acattatcat
    53941 gactggttac gtgacgccca cgcaatggaa aagcaagccg aatctatgct tgaatccatg
    54001 gccagccgta tagataatta tcctgaacta cgcgctcgta ttgaacaaca tcttagtgaa
    54061 accaaaaacc agattgttca actggaaact attcttgatc gtaatgacat ttcacgttca
    54121 gtcattaaag attccatgag taaaatggct gcgcttgggc agtcaatcgg tggtatattc
    54181 ccttctgatg aaatagttaa aggctctatt ggcggatatg tcttcgagca atttgaaatc
    54241 acctgttaca cctcactatt agcagcagca aaaaatgccg gtgatacagt ctcaattccg
    54301 atcatcgaag cgattttaaa tgaggaaaag caaatggccg actggctgat tcagcatatt
    54361 ccgcaaacaa ctgagaaatt tttaattcgt tctgaaactg atggcgtaga agcgaagaaa
    54421 taataccctt tataccatgt ccttattgac cccgtatatt acggggtcgt ttttgtgcgg
    54481 aattaaaaac gatatcctgc tgagaacata aacacccacg gatcgaggcg tacgctatcg
    54541 tgttgctgtg caccgcctag cttataattg gcggtggtat cgatatccat gtaccacact
    54601 gacatgttaa ccaaccagtc acggttaatc agataatcaa cccccacctg cccggcagct
    54661 ccccaggaat ctttcagact gagatcggaa agccccgcct ctttgccatg atcgttaaat
    54721 ccattatcaa agaaggtggt gtagttaata ccggccccaa cgtaaggacg gaatttgctg
    54781 ctggcatcac caaaatacca ctgcgccatc agtgttggcg gcagatggtg aacggttgca
    54841 atatcgccgg tcgcccgggt gccgatttta tggcggaacg gcgtcgctgc cagtaattcc
    54901 acaccaatgt tgtcggtcgc catataagta aacgtcaagc ccagttgagt gttattggtc
    54961 acgctgaatc cacccagact tcctaacgta ccaccagcac cttctgttgg acgtacggtt
    55021 gcagaacctg cacgcataaa aaattcgcct gcttcatgcg caaaggcact gccagaaaga
    55081 agagttgtta ctgccaaagc cgccactgtt aactttttca tatccgctcc gtcgttatgg
    55141 ttataaaaac gtgacgaata tacctacatt ggagtaacaa atgatcctac atagatcaca
    55201 ttacatatga taattcacca tttattgatc tggattaatt tagaagttgc attgagaatc
    55261 ctggttaatg tgatttaaat caatttttaa atattttctc tacgcaaata ttaactcgat
    55321 cgccacttgc cgcgctttgc ccacgcctga aggccagcaa ttacctccgc tttacaaaga
    55381 tatgcttttc catacaagac ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc
    55441 cgctaattaa cacctgcata aaactcaagg agagtgcatg tctatcacgg cgcagtccgt
    55501 ataccgtgac accggaaatt tcttccgtaa tcaatttatg accattctgt tggtatcgtt
    55561 gctatgtgcg tttatcacag tggtgttagg gcatgttttc tcacccagtg atgcacagct
    55621 tgcgcagctc aatgacggcg tgcccgttag cggcagtagt gggttgttcg acctggttca
    55681 gaatatgtca ccggaacagc aacaaatttt gctgcaggct tcagcggcgt ccactttttc
    55741 agaattaatc ggtaacgcca ttctcgccgg aggcgtaata ttaattatcc agctggtgtc
    55801 tgcgggtcag agagtcagtg cgctgcgagc tattggtgcc agtgcgccga tattgcccaa
    55861 gttatttatt ctgatttttc tgactaccct tttagtacag attggcatca tgctggtggt
    55921 cgttccggga attatcatgg ccattttact ggccctggca ccggtgatgc tggtacagga
    55981 caaaatgggc atttttgcct cgatgcgtag cagtatgcgg ctgacttggg cgaatatgcg
    56041 tctggtggca cccgcagtac tgagctggtt gctggcaaaa acactgttgc tgctttttgc
    56101 ctcttctttt gccgcattaa ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa
    56161 cctgatttct gccatattgc tcatctatct gttccgcctg tatatgttga ttcgccaata
    56221 accttaagca tttgattacg gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg
    56281 gttgtctttt tcgcgtttta caagatttat gacatctatg cggctactgc agcgctgatc
    56341 gtcgccacgg cgattgtgct tatctatagc tgggttcgct ttcgtaaggt tgagaagatg
    56401 gccctgatca cttttgttct ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat
    56461 gatgagttta ttaaatggaa ggttacagtc atttatgctc tgtttgcggg agccctgtta
    56521 gtcagccaat gggtgatgaa aaagccgcta attcagcgga tgctgagtaa agaactcacg
    56581 ctgccgcaac cggtatggtc gaagctaaat ctggcctggg ctgttttctt tatcctttgc
    56641 ggtctggcaa acatctacat cgcattctgg ctgccgcaaa atatttgggt caactttaaa
    56701 gtctttggcc tgaccgccct taccttaatc ttcacattgt taagcggtat ctatatctac
    56761 cgccacatgc cgcaggaaga taaatcctaa ccagactgcc agtccttcgg ggctggcgct
    56821 gtatctcctc tgttctcctc aggaaaatca tagtagcatc gcgcctgtga ttttcctttt
    56881 aagtcggttt taccatgtct acaacacata acgtccctca gggcgatctt gttttacgta
    56941 ctttagccat gcccgccgat accaatgcca atggtgacat ctttggtggt tggttaatgt
    57001 cacaaatgga tattggcggc gctattctgg caaaagaaat tgcccacggt cgcgtagtga
    57061 ctgtgcgggt tgaaggaatg actttcttac ggccggttgc ggtcggcgat gtggtgtgct
    57121 gctatgcacg ctgtgtccag aaagggacga catcggtcag cattaatatt gaagtgtggg
    57181 tgaaaaaagt agcgtctgaa ccaatcgggc aacgctataa agcgacagaa gcattattta
    57241 agtatgtagc ggttgatcct gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa
    57301 aagcctccgg tcggaggctt ttgactttct gcttactgaa tttcggtggt gccgttaatt
    57361 ttaaacagga tattcaccac aatcccactg cctggcttac ccggctcata acgccatctg
    57421 cgcatcgcat ttttcacctc acgctcaaac atgttcgcag gcttggctga gaggatttgt
    57481 acgttatcca cgcgaccatc cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg
    57541 cgcaatgcct gtgctcgtgc cggatactgc ggctgattac ggcttaatgc gcgtggtcct
    57601 gaagccacac tggtaaccgg cttgctggtt gcagccgttg ccgtacttga tgtggggcgc
    57661 gcgggtgccg tattttcaaa cggtgatgcc ggacgcgact ctacgggttt gacatcgcgt
    57721 tttggctgct cctgtacctt tttcaccggc ttcggttttg gcttaggttt cggcttcggc
    57781 ttttcaatga ccaccggtgc ttcttttggc ggttcgggga tcggctcagg ttccggttct
    57841 ggctctacca ccggctccgg tggcggctga acggcttgtg gcggttcgag atcagcaggc
    57901 gcaaccatcg tgacagaaat tggttgcgca ggcgcaggta gttcaataac ctgatgtacc
    57961 gaggtataga gcagccccgc cacaacagca ccatgaatgc agaccgaaag taacgtcggc
    58021 caggggaagc ggcgaggtaa atcaagggtt attgaagtca taatcatttc agtagaaaaa
    58081 ctaggcctcg attttaaatg caaatagcaa tcatattcaa taaggcaaga cgatcaggat
    58141 cagtgaaaat gtcatttttg gcgtaattac ccttacttta tgcatgacct taacaaagac
    58201 atcatctttc cgttgcagat cttcgccctt tcaaataacg tactttacaa ctttcccgaa
    58261 caaggagttg tgcccgtgtt gtatgttatt tacgctcaag acaaagctga tagcctcgaa
    58321 aaacgccttt ccgttcgtcc ggcacattta gcacgtttac agttactgca tgatgaaggt
    58381 cgtttgttga cagctggtcc aatgccagcg gtagacagta acgatccagg ggctgcggga
    58441 tttactggct caaccgttat tgctgaattt gaatcgctgg aagcagctca ggcctgggcc
    58501 gatgctgacc cgtacgtagc ggcaggagtc tatgaacacg tttcagtgaa accatttaag
    58561 aaagtgttct gag
//
LOCUS       DAETQL010000015        58032 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.015, whole genome shotgun sequence.
ACCESSION   DAETQL010000015 DAETQL010000000
VERSION     DAETQL010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.015"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(24..278)
                     /locus_tag="K0W65_000972"
     CDS             complement(24..278)
                     /locus_tag="K0W65_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBI3094343.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="K0W65_000973"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="K0W65_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBI3094344.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="K0W65_000974"
     CDS             1228..2694
                     /locus_tag="K0W65_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBI3094345.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="K0W65_000975"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="K0W65_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HBI3094346.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="K0W65_000976"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="K0W65_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBI3094347.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="K0W65_000977"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="K0W65_000977"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBI3094348.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="K0W65_000978"
     CDS             5709..7265
                     /locus_tag="K0W65_000978"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBI3094349.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="K0W65_000979"
     CDS             7347..9161
                     /locus_tag="K0W65_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBI3094350.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="K0W65_000980"
     CDS             9162..10256
                     /locus_tag="K0W65_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBI3094351.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="K0W65_000981"
     CDS             10256..11281
                     /locus_tag="K0W65_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3094352.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="K0W65_000982"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="K0W65_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBI3094353.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="K0W65_000983"
     CDS             complement(12876..13220)
                     /locus_tag="K0W65_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094354.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="K0W65_000984"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="K0W65_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HBI3094355.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="K0W65_000985"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="K0W65_000985"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBI3094356.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="K0W65_000986"
     CDS             15616..17376
                     /locus_tag="K0W65_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBI3094357.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="K0W65_000987"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="K0W65_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBI3094358.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="K0W65_000988"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="K0W65_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBI3094359.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="K0W65_000989"
     CDS             19113..19340
                     /locus_tag="K0W65_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBI3094360.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="K0W65_000990"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="K0W65_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBI3094361.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="K0W65_000991"
     tRNA            21195..21271
                     /locus_tag="K0W65_000991"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="K0W65_000992"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="K0W65_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HBI3094362.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="K0W65_000993"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="K0W65_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBI3094363.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="K0W65_000994"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="K0W65_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HBI3094364.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="K0W65_000995"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="K0W65_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBI3094365.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="K0W65_000996"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="K0W65_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HBI3094366.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="K0W65_000997"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="K0W65_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBI3094367.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="K0W65_000998"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="K0W65_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBI3094368.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="K0W65_000999"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="K0W65_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBI3094369.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="K0W65_001000"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="K0W65_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBI3094370.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="K0W65_001001"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="K0W65_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBI3094371.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="K0W65_001002"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="K0W65_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBI3094372.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="K0W65_001003"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="K0W65_001003"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBI3094373.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="K0W65_001004"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="K0W65_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBI3094374.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="K0W65_001005"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="K0W65_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBI3094375.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="K0W65_001006"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="K0W65_001006"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HBI3094376.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="K0W65_001007"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="K0W65_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBI3094377.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="K0W65_001008"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="K0W65_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBI3094378.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="K0W65_001009"
     CDS             37391..37555
                     /locus_tag="K0W65_001009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094379.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="K0W65_001010"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="K0W65_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBI3094380.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38300..39331)
                     /locus_tag="K0W65_001011"
     CDS             complement(38300..39331)
                     /locus_tag="K0W65_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HBI3094381.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39565..39924)
                     /locus_tag="K0W65_001012"
                     /pseudo
     CDS             complement(39565..39924)
                     /locus_tag="K0W65_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="K0W65_001013"
     CDS             complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="K0W65_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBI3094382.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="K0W65_001014"
     CDS             complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="K0W65_001014"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HBI3094383.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42511..43575)
                     /gene="ada"
                     /locus_tag="K0W65_001015"
     CDS             complement(42511..43575)
                     /gene="ada"
                     /locus_tag="K0W65_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBI3094384.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="K0W65_001016"
     CDS             complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="K0W65_001016"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBI3094385.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="K0W65_001017"
     CDS             complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="K0W65_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBI3094386.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46675..49347
                     /gene="rcsD"
                     /locus_tag="K0W65_001018"
     CDS             46675..49347
                     /gene="rcsD"
                     /locus_tag="K0W65_001018"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBI3094387.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49364..50014
                     /gene="rcsB"
                     /locus_tag="K0W65_001019"
     CDS             49364..50014
                     /gene="rcsB"
                     /locus_tag="K0W65_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBI3094388.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="K0W65_001020"
     CDS             complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="K0W65_001020"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBI3094389.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53338..54114)
                     /locus_tag="K0W65_001021"
     CDS             complement(53338..54114)
                     /locus_tag="K0W65_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBI3094390.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54119..55768)
                     /locus_tag="K0W65_001022"
     CDS             complement(54119..55768)
                     /locus_tag="K0W65_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HBI3094391.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55769..56328)
                     /locus_tag="K0W65_001023"
                     /pseudo
     CDS             complement(55769..56328)
                     /locus_tag="K0W65_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56332..56490)
                     /locus_tag="K0W65_001024"
     CDS             complement(56332..56490)
                     /locus_tag="K0W65_001024"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094392.1"
                     /translation="MTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGG
                     GEDWRWVG"
     gene            complement(56487..>58032)
                     /locus_tag="K0W65_001025"
     CDS             complement(56487..>58032)
                     /locus_tag="K0W65_001025"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBI3094393.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14197 a        15654 c        14259 g        13922 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttaacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcggacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ttttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggcc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gagatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gttaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatcat tacaacgcaa tatccgccac ttctttttga acgggttgcg
    38341 gtttcaactg cggtttcggc gtactatctg ctgcttgcgg tttgtcgtat ttcagcccca
    38401 gcacttcgct ggtgtactgc aactcgcgtt ctgttgccgc tacatcaccg ttcagcttgc
    38461 gtccataaga cggaacgatc gctttcaacg tagcctgcca ttgcgggctg gaaacgcgat
    38521 cgccaaatac tttttccagt agattcaaca taatcggcgc ggcggttgac gcccctggcg
    38581 atgcccccag cagcgccgca atggtccctt gctggtcact gaccacttcg gtacccagac
    38641 gcagtacgcc acctttctcg gcatcacgct tgataatctg cacacgctgc cccgcttgcc
    38701 acaaacgcca gtcctctttt ttcgcttgcg gatagtactc tttcaacgct tcaaaacgat
    38761 cctcttcact caacatcacc tgactcacca gatatttcac cagatcgaaa ttatccagcc
    38821 cgacgtgcat catcggcatc acgttagagg tggtggtgga actcatcaga tcccacaatg
    38881 aaccgttttt gaggaatttg gttgagaagg tggcaaatgg cccaaacagc actacgcgtt
    38941 taccgtccag aacacgggta tcgatatgcg gaaccgacat cggtggtgcg ccaacggatg
    39001 ctttaccgta aactttcgcc agatggcgat taaccacgtc tggattttcc gaaacaagga
    39061 actgtccgcc caccgggaaa ccggcgtagt ctttcgcttc cggaatcccc gattcctgta
    39121 acagcttcag cgccgcaccg cccgcgccga taaagacaaa tttggcgcgg atgttctgtg
    39181 cagtgccgtt tttcagatcg gcaacggtaa cggtccaggt gttatcgtca ttacgcttta
    39241 gggcgcggac ttcgctgctg agttgcagcg agaagttaga tttcttctgt aaggaagcaa
    39301 ttaactggcg ggtgatctca ccatagttca catcagtgcc aatttctgtt gcggatcgcg
    39361 cccttccatc actaacggtg cccactcttt gatctgcgcg tgatcttcag agtaacgcat
    39421 accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg gcgcgcagga aattaacgtt
    39481 atcctcgccc cagacaaagc tcatatgcgg aacggtattg ataaatgaac gcggagtacg
    39541 cagcacgccg cgctcgacct ggtgcgccca gaactggcgg gaaatctgaa atgcttcgtt
    39601 aatggcgact gctttttcaa tactgatgct gccatcggcg ttttgcgggg tgtagttcag
    39661 ttccatcagt gcagaatgcc cggttccggc gttgttctag ccgttcgaac tctcctgcgc
    39721 gcccccctcc aggcgctcca ccatggtcat cgaccattca ggctccagct cgcgtcaata
    39781 ggtccccaac gtggcgctca taatgccgcc gccaatcaac agtacatcag tttcctgctc
    39841 ctcggacgct ttcgcttttg ccgccatcga aacggcatta agccccacgg ccatcgagaa
    39901 gagcatggca gtcacttttt tcatcttgtt aatgccttac ttttagtcgc tttattgcag
    39961 gtgagatttg cgcggcatca acggtaacac ttaagtaaca acatttaaat aatgtttata
    40021 aattatattc taattttaga aacattataa tttcgttgat taattatagg gttattagaa
    40081 atgaagggat ttttagcata ttgacctgac ggcagcagtc cgccaggtca gcgtcgtaat
    40141 attatgccgt ccgggcaacg gcatcacgcg aagcggcatc gcgctcttcg cccgtcagct
    40201 cgctaagttg cccattgcgc atttccagca ggcggtcggc gtggataaag taatgatcat
    40261 catgactgat agcgaaaata gttttaccca tctcctgcat cagcggcagc aacacctgat
    40321 aaaactcacg acggaagtgt ggatcctgat ccaccgccca ttcatccagc aggataatat
    40381 cgcgttcttc tgccagcgcc agcaacagcg ccacgcgttt tttctgccct tttgataact
    40441 tcaggttaac aatacgcccg ttgcttaact caagcttatg agccattttc agctgcgcca
    40501 gccacttctc aaccagttgc gggttagcgg gttgcccctc cggccccagc agttgatcaa
    40561 acagccagac atcggtaaac actgccgaaa acagtttgcg ataatcttcc ggttgttcgc
    40621 cgctgacagg tttgccatcc agcaagattt cgccgctttg tggctgatac aagcccgtca
    40681 acaacatcgc cagcgtcgat tttccgctac cgttaccgcc aatcagaaac agcagctcgc
    40741 cacgtttgat ggtgagatta atcggaccaa cggaaaacgc gttatcctga taagcaaacg
    40801 tcacgttacg cagctccagc gtttgccagt tgggaaacgc ctgcgggcgc ggaaactcag
    40861 ctttgaaagg cgcgagcgcg aatttgttca gcttgttaaa cgccacctgc gccgtcagca
    40921 gcgtcggcaa tgcgccaacc gccgaaagca gcggcgtacg caggaataac agcgtcaacg
    40981 aataggtcgc agcaacgttg gtatcagccc aaccgagact gttcgccatc cagaacacca
    41041 ggccgatagc gcccagcatc atgatgtttg accagttcac ggcactaaga tggaaggtgt
    41101 ctgcgcgaat aatatgatgg cgatactctt gcgcatcggg gatgtagagg ttgttaaaca
    41161 catactcggc gcgttcccgg ttcagagtca gctctttgcg cccttcaagt acagtttgaa
    41221 aatccgtgta cagcttgtct tcggtttcac gtagggtcgc catatgtttg tacacccgcg
    41281 ccaccagcac aaaaccgccc cagatggtga tcgccatcca gatagccgtt accagcaaca
    41341 ttttgcccga cagcatccac agatacgccg atgaaccgat agtgagaatg atcccctgcc
    41401 ccagttccgg cagacgcaca aaagcaatgg tgatattgcg cacatcgctg gttaatcccg
    41461 ccagcaacga ggcgctaccg agttgttcaa tgcgctcgac gtgggtatcc agaatccgct
    41521 tgataaattc actacgcagt cggtaaacga agtgatgccc caaagtggtg agcgccagtt
    41581 gcgatccgag agtgactgcc atcaacagca gcaataatcc cagaaactcc ggtaacacca
    41641 gcagactggt atccgccgtt tcgataaggc gctgattgat aaaagcaatt aagccaatgc
    41701 ctaatgccgc actggcgagg cttagcgcca tcacactgat aaatggccag cgatactgcc
    41761 gccagacaag tacaagaagt tccatgcaga aaacccggac aatgaattac agcccgcagt
    41821 ttaaacatct tcgcgcgcac agcaataata attcttattt ttattctttt ttacctgcct
    41881 gacggaatgt caggttgtag cggcagtcgg tggtgagtgg atgaaacccc gctttcaacg
    41941 gttgaatacc gtgataaaac agccgcgatt caccgcccca taccaccaca tcgccatgtt
    42001 ccaacaacaa acgtttgagc ggatcattac gtttcaggcc accaaattga aaaatcgcgg
    42061 gtaagcccag agaaacagaa acaattggcg cgcgcagatc cggttcgtct ttatcctgat
    42121 gcagcgacag tttcgcgcca ggagcgtagc ggttgataag acaagcatct ggctggaaat
    42181 ctggatagcc cgccgccgta gccgcacgtt gacataaatc atgaaagctt tgtggcatag
    42241 cgggccacgg tttatttgtt tgcggatcaa tgggcgaata gagataacct tgccgatggg
    42301 tcgtccagcc cagatgccca cagttggtca tcgccaccga catggtatat ccgcctggag
    42361 cgaccatctg gcgaaacggc gactggctgg caacgtcatt aatatcgcgg attagttgct
    42421 ccgcagcatt aaaagcaaaa cgccgtaaaa ttaccgcacc agccgccagt ggctcttgcc
    42481 acggttcagc atcggcaaac agatccaaca ttacctctcc tcattttcag cttcgcggcg
    42541 cagcagttgc gctttacgcg acacgcccca gcggtaaccg gaaagtgtgc catcaccacg
    42601 gaccacccga tgacagggta ttacgatagc cagcttgttg gcggcacagg cgctggcaac
    42661 ggcccgtacc gctttcggtt tgccgatggc gttagccagt tgctgataac tgacggtttc
    42721 accacaaggt atcgtgcgca gtgcctgcca gacttgttgc tgaaaagcag tgccgcgaat
    42781 atccagcggt aacgtcagcg gcgtatcgcg ttgattgagg ctggcgatca cttcacgcac
    42841 atgttgctga aacgtcagat cggcaggcgc gttgtcggca gcgggaaaca tctgctgcaa
    42901 ctcgctgatt aatgtcgcgt catcatcgcc cagcaatatc gcgcaaatcc cccgctcgct
    42961 ttctgccacc aggcaacgac ccagctcaca atcagccagc gcgtaacgca ccgccagatt
    43021 ttcgccaccg tgacggaatt gtttagccgt catgcccagc gtttcatcgg ctttgcggta
    43081 gtaactgctg ctgtcgggga atccggcgtt aagaatagac gtcgtcacgc tctccccttt
    43141 cgccagcgat tcgcgcaaac ggcgagcgcg ccaggcctgt tgccaggctt taggcgtcat
    43201 tccggtagta gctttaaaca accgatgtag atgaaatgga ctcatcgcca cctggtcggc
    43261 taaggcttcc agcgttacag gcgtttcctg ttccagcagt cgacacgcgt gggtgatttt
    43321 atccaaccga tgttgccggg gattggcttt gtctggctga caacgtttgc aggggcgaaa
    43381 tccggcggcg agtgcctcgc tggcatttgc gtagaaggag acattttccc gcaaggcatg
    43441 tctggcgcgg caagaaggac ggcaaaagat acccgtggtg cgcacggcga aaacgaattc
    43501 gccgtcggca ttcgggtcgc gggctaagac agattgccag cgttgatcgt cagttaagca
    43561 tgtggcgttt ttcataatca gctccctggt taaggatagc ctttagtctg cccggtcacc
    43621 atcacgcaaa aaccaacaat cttgcgcttt aatttttttc gctgacaagg aagcttttaa
    43681 actgtggtga catccaggtt ttaaagctat cgccttcttt ggtgatcata taaaccgcca
    43741 gcccttcccg gcgaacaacc tctttggctt tctccggccc gagtaccatc aagccagtat
    43801 cccaggcatc ggcttccagc gccgtcggag caatcaccgt cacggatacc agattgtgtt
    43861 cgatgggacg cccggtttgc ggatcgataa catgggaaag acgtttgccg tccagttcgt
    43921 aatagttacg gtagctgcca gaggtgctaa tcccatgacc gttgatatcc accacagcct
    43981 gaaccgcgtt ttctttatcg gttggttttt gaatcgctac ccgccacggc tggccttcac
    44041 cgttcatacc acggctgttc agcgcgccgc ccaccgacac cagatagcgg gaaatccctt
    44101 cctgctccat caagcgtgcc aggtgatccg ccgcataacc ttcaccgacg gtggagagat
    44161 cgacatataa atccggcagg tctttttgca gatattgctg atgcgactga ttgatgaccg
    44221 tcaggtgctg taagccggtt ttggctttca tcgcatcgat ctgttcctgg ctcggaattt
    44281 gaaccggctg ttgttccggg ccaaagcccc acagattcac cagcggcccg acggtaatat
    44341 ccatcgcacc atcggtcctt gccccaatgc gcagcgaggt ggtgacgata tcggccatcg
    44401 cttcacttac cggccacggc gacaaacttt gcgagtcgtt aaagcgcatc agcgcggaat
    44461 cttttttata ggtcgaaagc agctgatcgt cggcgtccag ctgggtctga atcttttctt
    44521 taagttcggc actgcgtttg gcatcaatgc ccgggatgct ggcacgccag aaggtaccca
    44581 tggttttgcc ttcaagaacg gtaacttcag tagcgtgggt tttggcgggt tgtggttttt
    44641 gatcgcaacc aacaaagaag agcgcggcaa ccagcagtgc cacgcgggta aagcttattt
    44701 ccatacgtga ttatcctcat gcgaacggtc gcaagagtac accaaaaaac tgtgtttgta
    44761 cgctgaaaac aatgaaaaaa gggcccgcag gccctttgtt cgatatccat cgagattaga
    44821 actggtaaac cagacccaga gctacgatgt tatcagtgtt gatgccagcg tcacgagtga
    44881 actggttgtc gtccagcagg ttgattttgt agtcaacgta ggtggacatg tttttgttga
    44941 agtagtaggt cgcaccaaca tcaacatatt tcaggatatc ttcgtcgtcg tagttacgac
    45001 cattgatgac acccaggttt ttacctttag actgcaggta agccagggac ggacgcagac
    45061 cgaagtcgaa ctggtactga gcaacagctt cgaagttctg tgctttgttc gcccaaccca
    45121 agttgcctac gcgagttgcg ttgtaggtct gggtgtactg agcagctagg tagatgttgt
    45181 tcgcgtcgta tttcagacca ccggtgtagg tttcagcgcg gtcgccatta ccgatgtaac
    45241 gctcatcata acggttcaga ccgaagttct ggtcatcagt acgtttggag ctggaaactg
    45301 cagcaccgat accgaagcct tcgtaatcat aagtaataga accgccaacg ccgtcaccgt
    45361 tctgacgcag agcttcacga ccgttgttgg tcatgccttc accttccggg ctaccgtttt
    45421 tgccctgata ctgaacagcg aagttcagac cgtcaaccag accaaagaag tcggtgctac
    45481 ggtaggttgc gaagccgtta ccacgctgct gcatgaagtt gtcagaaccg taagtgtcac
    45541 caccgaattc tggcagtacg tcggtccagg aagttacgtc gtaaactacg ccgtagttac
    45601 gaccgtagtc gaaagaacct acatcctgga atttcagacc tgcgaatgcc acacgggtcc
    45661 aggagttgtt ttcgttttca gcgctgttgc cctggatctg atattcccac tggccgtaac
    45721 cggtcagctg gtcagtaacc tgagtttcac ctttgaagcc aagacgcatg taggtctggt
    45781 cgccatctac agacttgtcg tcagagaaat agtgcaggcc gtctacttta ccgtacagat
    45841 ctaatttgtt gccgtctttg ttgtaaactt cagcagcgtt tgctgcgcct gctaccagca
    45901 gagctgggac caggagggac agtactttaa ctttcatgtt attaaccctc tgttatatgc
    45961 ctttatttgc ttttttatgc cactgcatac tgattaaccc tcattaatca gtcggcaagt
    46021 ccattctccc caaaaatgca gaataatcca acacgaatat gatactaaaa cttttaagat
    46081 gtttcattta tcgctataga tgtttcaaaa tgtaaataca agggaacttt ttaagattat
    46141 tgcggaatgg cgaaataagc acctaacatc aagcaataat aattcaaggt taaaatcaat
    46201 aacttattct taagtatttg acagcactga atgtcaaaac aaaaccttca ctcgcaacta
    46261 gaataactcc cgctatcatc attaacttta tttattaccg tcattcattt ctgaatgtct
    46321 gtttacccct atttcaaccg gatgcctcgc attcggtttt tttacccttc tttacacact
    46381 tttcattatt ctgtgctacc acagaaaaac tataacgctt gttaactatt tcacaaataa
    46441 ttaacatcag cataatttcc agcaatcttt gtttatttgc aattaatttc gttgggcttt
    46501 ttgtaggtta tttgtacagc aaaatggcgc ttgtacatct atttcccccc atacaggatg
    46561 ataaatatca cgggagaata gagaatcatc aatcaggtaa gagtctggaa tttcacactg
    46621 taccctttat actgccctat cacttcgcga agttttaaca ggtcataaac acgaatgcgt
    46681 cagaaagaga caacggccac gacccgcttt tcactcctac cggggagcat tacccgcttc
    46741 tttttactgt tgatcattgt gttactggtg acgatggggg taatggtaca aagtgccgtt
    46801 aacgcttggc tgaaagataa aagttaccag attgtcgaca ttacccacgc aatccaaaag
    46861 cgcgtcgata cctggcgtta cgtgaccagg cagatctacg acaacattgc cgcgacgacc
    46921 tccccctcct ccggcgaagg tttacaagag acgcgcctga aacaggatgt ctactatctg
    46981 gagaaaccac gccgcaaaac ggaagcgtta atctttggct ctcacgacaa ctcaacgctt
    47041 gagatgactc agcggatgtc cacttatctg gatacattgt ggggtgcaga aaatgtaccg
    47101 tggtcgatgt attacctgaa tggtcaggat aacagtctgg tgctgatctc aaccctaccc
    47161 cttaaagatc tcacctccgg atttaaagaa tcgaccgtca gtgacattgt tgattcacgt
    47221 cgtgcagaaa tgttacaaca ggccaacgcc ctcgatgaac gcgaaagttt ttctaacatg
    47281 cgccgcctag cctggcagaa cggtcattac tttaccttgc gtaccacgtt caaccagccg
    47341 ggacatctgg caacggtcgt ggcttttgat ctgccgatta atgatttgat cccaccgggt
    47401 atgccgctgg acagtttccg ccttgagcca gacgcgacgg caacgggaaa caatgataat
    47461 gagaaagaag ggacggatag cgtcagtatc cactttaaca gtacgaagat tgaaatctcc
    47521 tcggcactca actctaccga tatgcgcctg gtctggcagg ttccttatgg caccttattg
    47581 ctggatacgt tgcaaaacat tctgctgcca ctgctgctga acatcggttt gctggcgctg
    47641 gcgttatttg gctataccac attccgccat ttctccagcc gcagtacaga aagcgtcccc
    47701 agcacggcgg tcaataacga attgcgcatt ttacgggcaa tcaatgaaga gatagtctca
    47761 ctgttgccgc tcggcctgct ggttcacgat caggaatcga accgcactgt cataagtaac
    47821 aaaattgccg atcatttgct gccgcatctg aatctgcaaa acatcaccac catggcggaa
    47881 cagcatcagg ggattattca ggcgacgatc aataacgagc tgtatgagat ccgcatgttc
    47941 cgcagccagg tcgcgccgcg cacacaaatt ttcattattc gcgatcagga tcgcgaagtg
    48001 ctggtaaaca agaaactcaa gcaggcgcag cgtctgtatg agaaaaacca gcaggggcgg
    48061 atgaccttta tgaaaaacat tggcgatgcg ctgaaagaac ccgcacagtc cctggcggag
    48121 agtgcggcta aactcaacgc cccggaaagc aaacaactgg cgaatcaggc agatgtgctg
    48181 gtgcggctgg tcgaagaaat acagttagcg aacatgcttg cggatgatag ctggaaaagt
    48241 gagacggtgc tgttctccgt gcaggattta attgatgaag ttgtgccttc agtgttgcct
    48301 gccatcaagc gtaaaggtct gcaactgctg attaacaatc atctgaaagc acacgatatg
    48361 cgccgcggcg atcgcgatgc cttacgacgt attttgctgc tactgatgca atatgccgtg
    48421 acctcaacgc aattgggaaa aatcaccctt gaggttgatc aggatgagtc ctctgaagac
    48481 cgcctgacat tccgcattct ggacacggga gaaggcgtaa gtattcatga aatggataat
    48541 ttgcacttcc cgtttatcaa ccagacccaa aacgatcgtt atggaaaggc ggacccgctg
    48601 gcattctggc tgagcgatca actggcacgt aaactgggcg gtcatttaaa catcaaaacg
    48661 cgggatgggc ttggtacacg ctactctgtg catatcaaaa tgctcgccgc tgacccggaa
    48721 gttgaagagg aagaagagcg tttactggat gatgtctgcg taatggtgga tgttacttcg
    48781 gcagaaattc ggaatattgt cactcgcctg ttagaaaatt ggggtgcaac ctgtatcaca
    48841 cccgatgaaa gattaattag tcaagattat gatatctttt taacggataa tccgtctaat
    48901 cttactgcct ctggcttgct tttaagcgat gatgagtctg gcgtacgaga aattgggcct
    48961 ggtcaattgt gcgtcaactt caatatgagc aacgctatgc aggaagcggt cttacaatta
    49021 attgaagtgc aactggcaca ggaagaggtg acagaatcgc ctctgggcgg agatgaaaat
    49081 gcgcaactcc atgccagcgg ctattatgcg ctctttgtag acacagtacc ggatgatgtt
    49141 aagaggctgt atactgaagc agcaaccagt gactttgctg cgttagccca aacggctcat
    49201 cgtcttaaag gcgtatttgc catgctaaat ctggtacccg gcaagcagtt atgtgaaacg
    49261 ctggaacatc tgattcgtga gaaggatgtt ccaggaatag aaaaatacat cagcgacatt
    49321 gacagttatg tcaagagctt gctgtagcaa ggtagcctat tacatgaaca atatgaacgt
    49381 aattattgcc gatgaccatc cgatagtctt gttcggtatt cgcaaatcac ttgagcaaat
    49441 tgagtgggtg aatgttgtcg gcgaatttga agactctaca gcactgatca acaacctgcc
    49501 gaaactggat gcgcatgtgt tgattaccga tctctccatg cctggcgata agtacggcga
    49561 tggcattacc ttaatcaagt acatcaagcg ccatttccca agcctgtcga tcattgttct
    49621 gactatgaac aacaacccgg cgattcttag tgcggtattg gatctggata tcgaagggat
    49681 cgtgctgaaa caaggtgcac cgaccgatct gccgaaagct ctcgccgcgc tgcagaaagg
    49741 gaagaaattt accccggaaa gcgtttctcg cctgttggaa aaaatcagtg ctggtggtta
    49801 cggtgacaag cgtctctcgc caaaagagag tgaagttctg cgcctgtttg cggaaggctt
    49861 cctagtgacc gagatcgcta aaaagctgaa ccgcagtatt aaaaccatca gtagccagaa
    49921 gaaatctgcg atgatgaagc tgggcgtcga gaacgatatc gccctgctga attatctctc
    49981 ttctgtgacc ttaagtccgg cagataaaga ctaatcatct gtaggccaga taagacgcgt
    50041 tagtgtctta tctggcattt gcaccgattg ccggatgcgg cgtaaacgcc ttatccggcc
    50101 tacgattccc attatttcaa cagattacat taaagtaggc cagataagac gcgtcagtgt
    50161 cttatctggc atttgcactg aatgccggat gcggcgtaaa cgccttatcc gtcctacgaa
    50221 tcccgcgatt tcctgaccct ctcggcatat accgtcagcg tctgttttat cacatccagc
    50281 gttaccggct tcgagaggca gctgtccata ccggactcca gacaccgctg cttctcttca
    50341 gccaacgcat tagcagttac tccgattacc ggcaacgtca gtcccaactg acgaatgcgt
    50401 tgcgtcaagc ggtaaccatc catatttggc atgttgacgt cgctaagcac gatatcaata
    50461 tgattcttac taagtacatt aagcgcatcg acgccatcat tcgcggtttt acattgatag
    50521 cccaacgatc ccaactgatc tgccagcaga cgtcggttaa tcggatgatc atccacgacc
    50581 agaatcatca tatcgtcgtt atcgctgacc gctttgtccg tcgacggcag agcgttagca
    50641 ggatcgtcgc tctccatctc gatcaaataa atacgcgcca acaatgccgg tagctcatgc
    50701 ggagcagcca cactgtgtac ccactcccct ggcgctttct ccagcggaat accaatatgg
    50761 cgacgacaga aggtcactac cgctctgccc tgccattttt tactcactac ctcgtcagtg
    50821 attaacacat cttcgggagt cggttcctgc ccttcgtatg ttgtaacgac gatgccgctg
    50881 cgctgcaaac tggtttccag gaactgacag agcgacgcat tgcggaccgc cagccagcag
    50941 cgtttaccac tcaacccttc cacgcctttt ttctgcgggt actgagcgcc gtacaacgga
    51001 atacgcacgg taaactggct gcccattccc ggttctgaat ctaccgagat atcgccgtcc
    51061 atcatgctga tcagtttttc acaaatcgcc agacccagac cggtcccctg gaagtttcgc
    51121 tgtacgcccg ttccaacctg gaagaaggga tcaaacaagc gcaccacttc tttcgccgga
    51181 atccccacgc cggtatcgcg aacacggata gagagataat cgccatcagc gcgaacatgc
    51241 aaaactatac agccggtatc ggtgaatttt atggcgttac tcaacaggtt ggagatgacc
    51301 tgctgtaaac gcatcgggtc gccatttaag gccactggca catccggttc aataaagcag
    51361 tacaagccta actgcttgcg taccaccagc ggtaaatagt tggcggtgat gtggttcatc
    51421 acttcacgcg gtgaaaactc acgcggttcg atcttcaact gttccgattc aatcttcgag
    51481 aaatcgagaa tatcgctgat aattttcaac aacaggctgg aagagttgtt cattgccgtc
    51541 accagacgat cgacgccttt cggtaactct ttggtttgca acagatccag gttaccgata
    51601 atgccataca gcggcgttcg cagctcatga ctgacggtgg caaggaacat cgattttgac
    51661 tggctcgcct gttccgctgc ttgtgccatc tcctgcaacg actcttccat cttcacgcgc
    51721 gaagaaacat ccaccagcac acaaatggcc acgttttcat tacgatagcg cgaatggacg
    51781 aagctgattt gcagattggt attgttgctg gtcaggacat caacaaaatt gacctgctgc
    51841 ccacagataa tttgcgtcag tcgttggcgg tcctcatgcg taagcatatt gagataggta
    51901 tgcgccagtt cgttacttaa aatattgacg ccatcagcgg tacgcaaaat gcagataccc
    51961 actggcgcgg aggcgacaat cttgcgattg aactgctcat gttcttccag tcgcagggcg
    52021 tcgctttccg ccggaatgaa aatacgtcgc tcgtacatcc gcgcgagagt aaacaatgca
    52081 gctccggcaa gcacattcag caaaattgcg ttaaggatca acatgcgaat gcgttccagc
    52141 accttatcaa ccggcaccga atacacgatg cttagcgatg agggtggcag atttttcttc
    52201 agcaccagct cccggaaccc ttccgtatag ccaaaccagg agcgttcctg catccagcga
    52261 ggatcgccct taattttact ttctggtccg gtaagcgaaa tcagggtatg accattttca
    52321 tcaagaatgg taacccccat cggcaacgta cccggtaaga aaaagttctc catccggatg
    52381 gtctgctcga cacccaaaag cgcctgcaac cggttcgcca gataaactgg cgtcaacgcg
    52441 taaaaatacc cgacgcaagg gcgcggacct tcgctgatcc agtagaggtt actgccgcta
    52501 tcatcttgtg gtgcatttcg atatttattg atgcgttcat gcaacgcttt caacgcggta
    52561 tcgcgttcca ctggcatatc acgcagaccg aaattggcca tgcagaggtt atcactgccg
    52621 attaaaaata cccggttgag atcgtaagcc gcagaaaaat tatcgcgcca gtagcgcata
    52681 aaccacgcca atgactccag agaacctcgc caggtgttac tcattgcgga acaatcggag
    52741 tcggcaaaca gcggttcaaa cgcaggcaca tccgcctgcg tttctcgtcc acgcggggaa
    52801 agcacaccgt tttctgccga taagcgattt tcggcgatgt acttaagctc tttcatcaca
    52861 tcagaggtgc gttgaataaa gcgctgagcc tgatcggaac tcagattaaa ttcctgacga
    52921 atttccgatt ctcgctgatg taacgcatta acgatgtaaa aaacggatga aaaagcaatc
    52981 aacagccaga gcactaacgc caatgctctg aacatgtagc gcgaggcttt cagggttgta
    53041 cgaaaagaag caaggtattt caaaggggag aagctccgcc tcaggtgacc gatggagtgt
    53101 ggttaaggta gcggtaaaag cgtgttaccg caatgttctg tcaccattgc aataatgcgt
    53161 atcgcttaga aaccattgcc ttaaccagga aagccggagt agatggacag ttctcagtac
    53221 gaaaaataag aaatgaccaa tctgccgacg cttgttaatg gttatatcct ggcgtcggca
    53281 gatcgcccag cagtcttatc tgaaaaaaga gagagccagg ttgtgtgaag ggatggatca
    53341 ccagtcaaaa cttttcacca gcgtcagctc gccagcatta cgcatcggta caataaatgt
    53401 ttcctgtttc tcattgaccg atccttcatc ggtgatcagc gtcagttggg cggtcgtcaa
    53461 ttccgtttcg ctgcgcccac catagtagtt gatatacacc tgatagcgcc cgtgaactgg
    53521 tgctggcatg gcgaaaatct cgggtccgta ccccgtcgtg acatccatat ccagtgcacc
    53581 actgtttttc agcacggtgt taccgtacca ggcgtgttcg ccatcgggcg tgacaacatg
    53641 gagatcgaga tcggtattgt ccgtatccca cgagagaacc agccgtaaac gtgcacgaat
    53701 agttcctgta cccggcgttg agtaaaactg cattttttgc cggctttgcc catccgggct
    53761 gatgacctgc acgctgttgc tgccttcagt gaaaatataa gggcgtgcaa atgaaccatc
    53821 ggattcaatt ctctgcggca tactggcacc gttgaccacc agccggccct gctgaacttt
    53881 acccgccgca gcataattct ttattttgcc gcggatctgc gctgagatat tttgatcatc
    53941 tgccatgttg acggacgacg ccgggtaatt gatcgattgt gaaaaactgg catcttcgcc
    54001 ctctgccgga tgccagccag agagcggtgc gtcaatctcg acttcctgca caccttcact
    54061 gtgagcgaca ggcgaaagtg ccaccagtaa caacggaaga aaaatctttc gcatagcatt
    54121 agtccattaa taattgtcgg gtcagcgttt cgatatagtt ctcatcaagc ccggtgggat
    54181 aaccatcaaa ggccaagtgc agatactcat gggttaaatc cagccgatcc tgaagcgtaa
    54241 agaagttacg aataaacagc cgtttctgct gccgatcggt ataggggaaa cctgagacta
    54301 aacgacagac cgcaaacacg tctggttcgt tgtaccccgt ctcgccttgt aatatacgcc
    54361 gccactgcgg catttttttc gccagccagg ctttcgcttt gggtaataac tggcaggttg
    54421 agcgtggagc tccccagcgg ctaaggctgt tgtcgggata agcaaacgcg agtatctggt
    54481 cgtaacgctc cccctgcccc gcctgggccg tggcttgccg ccaggaaagt gtcccttcgg
    54541 tagcgcgact gccatgataa tgaaccggat cgcctgcata gatgagctcc tgcgtccagg
    54601 cggtcatcgt tcgcgccccc gtggtcgctg gcgaagcaga aacacgctgc gtggcgctgc
    54661 tgtcggggat ggtcaggcaa tcaccttccc gattggcgtt ttgctgtaaa aaagtacgta
    54721 tcgccacagt catggctttc gctgcctcag gtggcgtact tttcgcctcg cgatccagaa
    54781 ctctggcaac gtactcttca cgatcgagat gcgactgcaa tttcagtttg cccttttcgc
    54841 tcagtaaggt cgtctcaccg tggctgacaa acgtaatgtg gttaccatta gtaaacgtca
    54901 cacgatagcg cccattaagt acgctgggtt ttactgccgt cgtactcttt tccgctgtga
    54961 ttttcttcag cggatagcgg gcaaacagct ccacctctac acattgccca ctggcaacct
    55021 gcgtcggcac tggcaaaacc cgattaagca ccgtcgcata acgggttagt accgttttgc
    55081 tggtgccact gccagtaacc cacagcggtg taccatcggt taaccagcca gcaaaaccac
    55141 cctgacggat ttcctgttta tcatcggcaa accagctcca ggttttcacc cgtacccgac
    55201 taccgagcat ggatgcgaca ccgattttcg cctcgtccag caccacatcc agtaagactt
    55261 cctgagcttt gttttgcgca ggtaacgtcg ctaatgaatc aagcagcgat ttaaccgtta
    55321 ccgaggtttc tggcttcagc gttgtaagag acgctaacca cgcaggtgcc tgacgctgtt
    55381 gccagtattg cccccacaca tctgcgccaa tatgcaaccg ctgcggcgca aaatagagcc
    55441 cacaggaacg taccagcgcg gtatcgcgcg ttatgctctc cccagcctga caacaataaa
    55501 cctcttccgg ggagtttccg cgacactggt agggctgctc cggttgatgg gtatcctcca
    55561 gccaggcgta aacatacagt ttccacaaac tccctaacgg cgtggtgagc gagtcaggta
    55621 atgctgagac tttggtcacg cctgatgacg acagctgata cagttgatcg tgctgcgcac
    55681 cgcgcagcac cagttgtaac ggcgtctcct ccgcaagcgt tggcagagta accaacgcca
    55741 atagccagac gattctgcgc cagttcactt atttcacctg catcccggtc cattcactcc
    55801 ctgccgcgac actttgctgc gcaggtgcat aggaacgcac ataacgagca ggaggcagaa
    55861 cgaattgccc tttttgcgag aagcgcagca aatggcggaa agtgaccgtt cccgtcagtt
    55921 ctttcaccgg caccatatag gccagttcgc ccatttcatt acgcgctttt tccagcaatt
    55981 gcccctgctg tttcgcggcg ttgggtttat tgactgaaat gccccatgtt gtgcgctcaa
    56041 cgtcagctcc cggcgggagc ggtacttcca cctgaccata gcgcagaact gcatcctgct
    56101 cgctggtaag cgtaatttca tcgagataca gcgcatcgct gtcaatctca ttgctggtca
    56161 ccggctgcag agtaaagctc atctcttctt caccggggat aagccgatac aactggcgtt
    56221 caacggtcac cggaatgtta ctttgttgag ccgtttttgc cggttcacgc cagcggacct
    56281 gcacatttgc ggcgataatt cgtcaccaaa agagagaatg tccggcacac cctaaccaac
    56341 ccaacgccag tcttcgcccc ctccagttaa cttatgttta gcccatgcgc ccgcaggcgc
    56401 aggcaaaaca actggaggca ttgtcgccat atatttcgcc agccagttca tggccagcgc
    56461 gcgctcaata gtggattgct cagcggtcaa accattaaaa tagcggccgt ctgggtagcg
    56521 tcaccaccgc tattgagcag cacaaccgtg tgcgccagcg gctgattgct gtttgccgct
    56581 ttatcccacg catattgctg aacctgtggc atcaccgtgg acttcaactg cgcttttttc
    56641 agcaacgtca tggttaagac gtttgccacc gcatctgcca gtggagattc cggtgtatcg
    56701 aggatcaggc tgtcattgat atcgcggatg tcttcctcag agaaatcttc atctttagtt
    56761 ccgcgccggg cgatagcttc atccccccct ttcaacaacg ttttgcacgg cagattcatc
    56821 tcctgtgccc acgccagcac cagcgcccga tgcaataacg gcatattatc cgcctgctcg
    56881 gcataactgt cgagcatatg ctgccagtat tccggttgtt gcgttacgcc gagcgcctgg
    56941 ctggcctgcc agtcggcgta ccatgcccat gccgtaagga aggcgtcacc attgccatct
    57001 tcaccccacc aggtaaagcg cgctccgggc cccgccagtt gcatcagccg cagacggtta
    57061 tcctgaatca tctgacgaat gtcgttagcg gcggcacttt gatgatcggc aagcgaacgc
    57121 caggcgagac tgagcgggat cagacggcta ccggtgttga tcaccccacc ccacggttca
    57181 tcgactaacg catcaagatt gttgcggaaa atctcctgcg gcgtttcact actttgtagc
    57241 cggatattac tcgcctgctc gggcaacatc agcgcgttct cgccaccgcc gagcatgaca
    57301 tttttctgct gttcaacggg ccagctgttg tccacaaaag acagttttgt gctaatgctg
    57361 tcctgcactt gcccattttg ttgcagttct gcacttaaca agccagattg ctgaatattc
    57421 tgcgtcagcg aaatataatt cgcccctttg tgcagcgtca gcgtctggcg catctcagcg
    57481 cctgcaaatt tagtcaccag cgccaccggt tcgttatcct gctgactgaa gataaacagt
    57541 cctgccgccg gtttgtcgcc cacgcgatac accgttggca tactccactt catgtagaga
    57601 tttttttccg aacgcagata agcacgcccc tgcccgacca gcccgtcgcc gttcatccca
    57661 cgcgcggtga tacgccagcg ggttaacgaa tcaggcatca ggaacgtgaa atacgctttg
    57721 ccttgtttat cggttgtgag tgacggcatc catgccgcgg tatccacctc ttcacgccgt
    57781 ggacgttcaa gcatttttac tcgccgctcg ctgcggttag tcgcgccagg cgcaaccggc
    57841 tcgctggaga gcgcctggtc atagctgata aacgacagac tggagctggt acgcacattg
    57901 ttacgcccca gcggatagaa aaatttgccg atattcggcg cgatttctgg ttgcagcgcg
    57961 tagatcattt catcgaccac gcctaccgtt agctgcgcag aaacaggttt acctttcagc
    58021 gacgaggtta at
//
LOCUS       DAETQL010000016        55611 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.016, whole genome shotgun sequence.
ACCESSION   DAETQL010000016 DAETQL010000000
VERSION     DAETQL010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55611
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.016"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..441)
                     /locus_tag="K0W65_001026"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="K0W65_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(416..1216)
                     /gene="otsB"
                     /locus_tag="K0W65_001027"
     CDS             complement(416..1216)
                     /gene="otsB"
                     /locus_tag="K0W65_001027"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HBI3094394.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1383..2369)
                     /gene="araH"
                     /locus_tag="K0W65_001028"
     CDS             complement(1383..2369)
                     /gene="araH"
                     /locus_tag="K0W65_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HBI3094395.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2384..3898)
                     /gene="araG"
                     /locus_tag="K0W65_001029"
     CDS             complement(2384..3898)
                     /gene="araG"
                     /locus_tag="K0W65_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HBI3094396.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3968..4957)
                     /gene="araF"
                     /locus_tag="K0W65_001030"
                     /pseudo
     CDS             complement(3968..4957)
                     /gene="araF"
                     /locus_tag="K0W65_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5754..6257
                     /locus_tag="K0W65_001031"
     CDS             5754..6257
                     /locus_tag="K0W65_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBI3094397.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6337..6588)
                     /locus_tag="K0W65_001032"
     CDS             complement(6337..6588)
                     /locus_tag="K0W65_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBI3094398.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="K0W65_001033"
     CDS             complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="K0W65_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBI3094399.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7052..7375
                     /locus_tag="K0W65_001034"
     CDS             7052..7375
                     /locus_tag="K0W65_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HBI3094400.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7546..8043
                     /gene="ftnA"
                     /locus_tag="K0W65_001035"
     CDS             7546..8043
                     /gene="ftnA"
                     /locus_tag="K0W65_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBI3094401.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8080..8319)
                     /locus_tag="K0W65_001036"
     CDS             complement(8080..8319)
                     /locus_tag="K0W65_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBI3094402.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8509..9720
                     /gene="tyrP"
                     /locus_tag="K0W65_001037"
     CDS             8509..9720
                     /gene="tyrP"
                     /locus_tag="K0W65_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBI3094403.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="K0W65_001038"
     CDS             complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="K0W65_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBI3094404.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10643..10729)
                     /locus_tag="K0W65_001039"
     tRNA            complement(10643..10729)
                     /locus_tag="K0W65_001039"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10693..10695),aa:Leu,seq:taa)
     gene            complement(10742..10815)
                     /locus_tag="K0W65_001040"
     tRNA            complement(10742..10815)
                     /locus_tag="K0W65_001040"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10781..10783),aa:Cys,seq:gca)
     gene            complement(10870..10945)
                     /locus_tag="K0W65_001041"
     tRNA            complement(10870..10945)
                     /locus_tag="K0W65_001041"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10910..10912),aa:Gly,seq:gcc)
     gene            complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="K0W65_001042"
     CDS             complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="K0W65_001042"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBI3094405.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="K0W65_001043"
     CDS             complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="K0W65_001043"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBI3094406.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="K0W65_001044"
     CDS             complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="K0W65_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBI3094407.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14483..14659
                     /locus_tag="K0W65_001045"
     CDS             14483..14659
                     /locus_tag="K0W65_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094408.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14646..14870
                     /locus_tag="K0W65_001046"
     CDS             14646..14870
                     /locus_tag="K0W65_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBI3094409.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="K0W65_001047"
                     /pseudo
     CDS             complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="K0W65_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="K0W65_001048"
     CDS             complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="K0W65_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBI3094410.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="K0W65_001049"
     CDS             complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="K0W65_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBI3094411.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="K0W65_001050"
     CDS             complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="K0W65_001050"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBI3094412.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="K0W65_001051"
     CDS             complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="K0W65_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3094413.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="K0W65_001052"
     CDS             complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="K0W65_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBI3094414.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="K0W65_001053"
                     /pseudo
     CDS             complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="K0W65_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="K0W65_001054"
     CDS             complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="K0W65_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBI3094415.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22563..23969
                     /gene="fliD"
                     /locus_tag="K0W65_001055"
     CDS             22563..23969
                     /gene="fliD"
                     /locus_tag="K0W65_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBI3094416.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23994..24404
                     /gene="fliS"
                     /locus_tag="K0W65_001056"
     CDS             23994..24404
                     /gene="fliS"
                     /locus_tag="K0W65_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBI3094417.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24404..24769
                     /gene="fliT"
                     /locus_tag="K0W65_001057"
     CDS             24404..24769
                     /gene="fliT"
                     /locus_tag="K0W65_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBI3094418.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24847..26334
                     /gene="amyA"
                     /locus_tag="K0W65_001058"
     CDS             24847..26334
                     /gene="amyA"
                     /locus_tag="K0W65_001058"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBI3094419.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26368..26781)
                     /locus_tag="K0W65_001059"
     CDS             complement(26368..26781)
                     /locus_tag="K0W65_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI3094420.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26968..28173
                     /gene="yedE"
                     /locus_tag="K0W65_001060"
     CDS             26968..28173
                     /gene="yedE"
                     /locus_tag="K0W65_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBI3094421.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28170..28403
                     /gene="yedF"
                     /locus_tag="K0W65_001061"
     CDS             28170..28403
                     /gene="yedF"
                     /locus_tag="K0W65_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBI3094422.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28510..29180
                     /locus_tag="K0W65_001062"
                     /pseudo
     CDS             28510..29180
                     /locus_tag="K0W65_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(29220..30428)
                     /locus_tag="K0W65_001063"
                     /pseudo
     CDS             complement(29220..30428)
                     /locus_tag="K0W65_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            30496..31111
                     /gene="tnpA"
                     /locus_tag="K0W65_001064"
                     /pseudo
     CDS             30496..31111
                     /gene="tnpA"
                     /locus_tag="K0W65_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            31439..32521
                     /locus_tag="K0W65_001065"
     CDS             31439..32521
                     /locus_tag="K0W65_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HBI3094423.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(33010..33808)
                     /locus_tag="K0W65_001066"
                     /pseudo
     CDS             complement(33010..33808)
                     /locus_tag="K0W65_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(33818..34369)
                     /gene="ybcL"
                     /locus_tag="K0W65_001067"
     CDS             complement(33818..34369)
                     /gene="ybcL"
                     /locus_tag="K0W65_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HBI3094424.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(34538..34870)
                     /gene="emrE"
                     /locus_tag="K0W65_001068"
     CDS             complement(34538..34870)
                     /gene="emrE"
                     /locus_tag="K0W65_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HBI3094425.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(35215..35529)
                     /gene="fliE"
                     /locus_tag="K0W65_001069"
     CDS             complement(35215..35529)
                     /gene="fliE"
                     /locus_tag="K0W65_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBI3094426.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            35743..37402
                     /gene="fliF"
                     /locus_tag="K0W65_001070"
                     /pseudo
     CDS             35743..37402
                     /gene="fliF"
                     /locus_tag="K0W65_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            37395..38390
                     /gene="fliG"
                     /locus_tag="K0W65_001071"
     CDS             37395..38390
                     /gene="fliG"
                     /locus_tag="K0W65_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBI3094427.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            38383..39069
                     /gene="fliH"
                     /locus_tag="K0W65_001072"
     CDS             38383..39069
                     /gene="fliH"
                     /locus_tag="K0W65_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBI3094428.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            39069..40442
                     /gene="fliI"
                     /locus_tag="K0W65_001073"
     CDS             39069..40442
                     /gene="fliI"
                     /locus_tag="K0W65_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBI3094429.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            40464..40805
                     /gene="fliJ"
                     /locus_tag="K0W65_001074"
                     /pseudo
     CDS             40464..40805
                     /gene="fliJ"
                     /locus_tag="K0W65_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            40802..41929
                     /gene="fliK"
                     /locus_tag="K0W65_001075"
     CDS             40802..41929
                     /gene="fliK"
                     /locus_tag="K0W65_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBI3094430.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            42034..42498
                     /gene="fliL"
                     /locus_tag="K0W65_001076"
     CDS             42034..42498
                     /gene="fliL"
                     /locus_tag="K0W65_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBI3094431.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            42503..43507
                     /gene="fliM"
                     /locus_tag="K0W65_001077"
     CDS             42503..43507
                     /gene="fliM"
                     /locus_tag="K0W65_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBI3094432.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            43504..43917
                     /gene="fliN"
                     /locus_tag="K0W65_001078"
     CDS             43504..43917
                     /gene="fliN"
                     /locus_tag="K0W65_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBI3094433.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            43920..44285
                     /gene="fliO"
                     /locus_tag="K0W65_001079"
     CDS             43920..44285
                     /gene="fliO"
                     /locus_tag="K0W65_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBI3094434.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            44285..45022
                     /gene="fliP"
                     /locus_tag="K0W65_001080"
     CDS             44285..45022
                     /gene="fliP"
                     /locus_tag="K0W65_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBI3094435.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            45032..45301
                     /gene="fliQ"
                     /locus_tag="K0W65_001081"
     CDS             45032..45301
                     /gene="fliQ"
                     /locus_tag="K0W65_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBI3094436.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            45310..46095
                     /gene="fliR"
                     /locus_tag="K0W65_001082"
     CDS             45310..46095
                     /gene="fliR"
                     /locus_tag="K0W65_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HBI3094437.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            46380..47003
                     /gene="rcsA"
                     /locus_tag="K0W65_001083"
     CDS             46380..47003
                     /gene="rcsA"
                     /locus_tag="K0W65_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBI3094438.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(47047..47235)
                     /gene="dsrB"
                     /locus_tag="K0W65_001084"
     CDS             complement(47047..47235)
                     /gene="dsrB"
                     /locus_tag="K0W65_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBI3094439.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            47398..47625
                     /gene="yodD"
                     /locus_tag="K0W65_001085"
     CDS             47398..47625
                     /gene="yodD"
                     /locus_tag="K0W65_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBI3094440.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            47923..48738
                     /locus_tag="K0W65_001086"
                     /pseudo
     CDS             47923..48738
                     /locus_tag="K0W65_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(48735..50429)
                     /gene="yedQ"
                     /locus_tag="K0W65_001087"
                     /pseudo
     CDS             complement(48735..50429)
                     /gene="yedQ"
                     /locus_tag="K0W65_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(50600..50782)
                     /locus_tag="K0W65_001088"
     CDS             complement(50600..50782)
                     /locus_tag="K0W65_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBI3094441.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(50861..51778)
                     /locus_tag="K0W65_001089"
     CDS             complement(50861..51778)
                     /locus_tag="K0W65_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBI3094442.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            51951..52871
                     /gene="yedA"
                     /locus_tag="K0W65_001090"
     CDS             51951..52871
                     /gene="yedA"
                     /locus_tag="K0W65_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HBI3094443.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(52860..53330)
                     /gene="vsr"
                     /locus_tag="K0W65_001091"
     CDS             complement(52860..53330)
                     /gene="vsr"
                     /locus_tag="K0W65_001091"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HBI3094444.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(53311..54729)
                     /gene="dcm"
                     /locus_tag="K0W65_001092"
     CDS             complement(53311..54729)
                     /gene="dcm"
                     /locus_tag="K0W65_001092"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HBI3094445.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(54796..55336)
                     /locus_tag="K0W65_001093"
                     /pseudo
     CDS             complement(54796..55336)
                     /locus_tag="K0W65_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(55376..>55611)
                     /locus_tag="K0W65_001094"
     CDS             complement(55376..>55611)
                     /locus_tag="K0W65_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094446.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT        13907 a        14312 c        13455 g        13937 t
ORIGIN      
        1 caatgcgatt attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat
       61 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
      121 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat
      181 aatgaaaagc gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
      241 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt
      301 ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat
      361 gccaacggca aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga
      421 tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg
      481 cggcggttat catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg
      541 cctgagttgc acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga
      601 agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag
      661 cttcctgcat aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga
      721 caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta
      781 atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc
      841 ctttcgcctc cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa
      901 tatcacgcgc aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc
      961 gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct
     1021 ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct
     1081 gtagtccttg cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg
     1141 ccagcgttcc atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg
     1201 ttaacggttc tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc
     1261 gctttacaga catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca
     1321 gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag
     1381 catcagacag tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg
     1441 attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt
     1501 tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct
     1561 ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac
     1621 gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc
     1681 aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc
     1741 gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc
     1801 agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac
     1861 cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct
     1921 gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca
     1981 tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac
     2041 aaacccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg
     2101 acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag
     2161 aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata
     2221 ttaatgaagg tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac
     2281 accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg
     2341 ccagaccccg atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc
     2401 tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt
     2461 caccggcgat ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag
     2521 gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt
     2581 tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga
     2641 ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga
     2701 gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat
     2761 cggcattgtt ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc
     2821 tggcactgat gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc
     2881 gagcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga
     2941 tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt
     3001 tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc
     3061 gaaccgccag acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc
     3121 gctcctcgcc ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca
     3181 tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac
     3241 gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag
     3301 aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc
     3361 cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac
     3421 gcgccagcgc tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg
     3481 gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca
     3541 gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg
     3601 cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa
     3661 gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg
     3721 ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac
     3781 ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa
     3841 cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa
     3901 cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc
     3961 cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg
     4021 tgatcagtac cacgtctgta acttcagtaa attttggcgt ttcaacgtct tttgctaccc
     4081 agttgtaaag catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac
     4141 cgtagaagcc ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac
     4201 caataccgat gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa
     4261 gtacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca
     4321 gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat
     4381 aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc
     4441 ggcgggcggt atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc
     4501 agccacgttt ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg
     4561 ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt
     4621 catccactgc aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga
     4681 gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga
     4741 tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat
     4801 ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct
     4861 tcaccagaaa accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca
     4921 gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg
     4981 ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc
     5041 ttttatctaa aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat
     5101 aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa
     5161 gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata
     5221 agattagcct ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc
     5281 agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca
     5341 aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta
     5401 acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc
     5461 acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat
     5521 taaaattcat tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat
     5581 gatttaagca ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa
     5641 ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat
     5701 ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa
     5761 ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc
     5821 tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc
     5881 ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga
     5941 gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc
     6001 tggaagaact gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc
     6061 agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc
     6121 tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca
     6181 gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt
     6241 cacaccagct gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag
     6301 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
     6361 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     6421 ggcagcggga tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     6481 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     6541 taccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     6601 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     6661 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     6721 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     6781 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
     6841 atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt
     6901 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
     6961 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa
     7021 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     7081 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     7141 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     7201 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat
     7261 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     7321 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     7381 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
     7441 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
     7501 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
     7561 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
     7621 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
     7681 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
     7741 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
     7801 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
     7861 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct
     7921 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
     7981 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
     8041 taatgctctc cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact
     8101 ctgatcggtc gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc
     8161 taccagctct cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc
     8221 aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat
     8281 catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc
     8341 tagcgtagcg attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt
     8401 gtacatttat atttacacaa tatgtaacgt cggtttgacg aagcagccgt tataccttaa
     8461 cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga
     8521 accctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg
     8581 ccgctggctg cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg
     8641 gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat
     8701 accggtctgg gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc
     8761 ttcagtatga tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa
     8821 ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg
     8881 ctgttgttca cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta
     8941 tttaaccgtt ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg
     9001 ctaccgcata ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg
     9061 tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc
     9121 agctatatgg atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc
     9181 cccctggtgg catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc
     9241 tttatgggat tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa
     9301 atggtggcct ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc
     9361 acgtcatttc tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt
     9421 tcaaataccg ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg
     9481 tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg
     9541 gcggtactgg cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct
     9601 caggcgggtt accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt
     9661 attgctgtga ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga
     9721 tcagatagcc tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct
     9781 cttaatgcag gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac
     9841 gccccggttt ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca
     9901 tccagtatgc atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg
     9961 cttctggcga cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg
    10021 cctccagcgc tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc
    10081 gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca
    10141 agccgaataa cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca
    10201 tatgttgaaa agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca
    10261 catagtcagc cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag
    10321 aactcaacac cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag
    10381 tgttgtattt ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg
    10441 ttttcatacg cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc
    10501 gtatcagaga tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg
    10561 acaagttgca ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt
    10621 gattttattc ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa
    10681 cgccgaggga ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa
    10741 gtggaggcgc gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg
    10801 ctttgttaac gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt
    10861 cttttaaact ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt
    10921 gctctaccaa ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat
    10981 tatcgtctgg caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg
    11041 agtcaacgat atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac
    11101 tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt
    11161 accgcagcca caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac
    11221 agcagccagg ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg
    11281 gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt
    11341 tcacgggcga tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt
    11401 accagcacca tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca
    11461 aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc
    11521 gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc
    11581 aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat
    11641 tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac
    11701 atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt
    11761 ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca
    11821 acatttgccg acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga
    11881 ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat
    11941 gctggataac atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct
    12001 caaagaacag cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca
    12061 gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa
    12121 gctggccttt gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt
    12181 taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc
    12241 ctgtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac
    12301 aggaagcgac ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca
    12361 cttccggcaa tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct
    12421 gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg
    12481 ccctatcgcc gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg
    12541 aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg
    12601 tgcgcatctg gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca
    12661 gttccgtacc gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac
    12721 gaatgaacaa tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa
    12781 ggtcgtcgcc ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt
    12841 ggtcgcgaat acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc
    12901 taatgagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga
    12961 cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc
    13021 ctatctggta ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc
    13081 gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga
    13141 acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt
    13201 gggtatcgcc actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt
    13261 aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat
    13321 gcgtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag
    13381 cgaggttgct gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat
    13441 agataaccgt accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt
    13501 ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca
    13561 cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact
    13621 gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat
    13681 ctcattgacc ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa
    13741 actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc
    13801 gatatcagaa gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc
    13861 ctgcggagcc gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg
    13921 taaagggttt tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc
    13981 gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac
    14041 gtcaacggca tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac
    14101 gacttttata cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg
    14161 gtcatcaaca agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact
    14221 gatagttagt cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt
    14281 gggtattcga tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc
    14341 gtcaccaacg gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat
    14401 tgactttatt cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga
    14461 acaatacggg caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt
    14521 ttatagcact gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat
    14581 ggtatactcc gccgccttaa catttttcac cacaaatttt cattgcaaca tgacgaggaa
    14641 aataaatgag tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag
    14701 tctcctgcct gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg
    14761 gccgcgtgat gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg
    14821 cagtattttc caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg
    14881 gctgagagcg tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct
    14941 tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg
    15001 tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact
    15061 gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa
    15121 aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta
    15181 aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc
    15241 acattaaatg gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat
    15301 tttttgcctg ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag
    15361 gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct
    15421 gagcctgaag ctctatttca tggtaggcct cttctgcggc ctccatcctc tgaaaacgca
    15481 acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc
    15541 ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa gatttatata
    15601 taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat
    15661 taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg
    15721 acaggtgaat cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac
    15781 tggcgggtgc gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact
    15841 atccgcccct ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg
    15901 tgtgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact
    15961 tcgcccacca gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga
    16021 cgcattgcca gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt
    16081 ggatagctgg tttctttacc ggccagccca actttcgcca gcaactcgcg agcgcgcgcc
    16141 gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc
    16201 accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac
    16261 tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca
    16321 acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt
    16381 ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg
    16441 ataccgtgca gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca
    16501 ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg
    16561 ataacacagt cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct
    16621 ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg
    16681 cggccagcga cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca
    16741 cccgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac
    16801 tggcagcggc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg
    16861 cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt
    16921 cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac
    16981 cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc
    17041 gcatcagcgc gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt
    17101 gcagggtata cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac
    17161 tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc
    17221 gccgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc
    17281 gtcaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag
    17341 acgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc
    17401 aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat
    17461 cgcctgttgt aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac
    17521 gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt
    17581 tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg
    17641 cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac
    17701 cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagtt cttccagttg
    17761 ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa
    17821 caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag
    17881 cgccacgcag tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga
    17941 ctggatcgcg ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag
    18001 aaattccagc ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt
    18061 gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg
    18121 cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc
    18181 cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc
    18241 gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac
    18301 catttttcgg aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc
    18361 accgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag
    18421 aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc
    18481 agacgatcaa cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg
    18541 tcatcatcat aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag
    18601 ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg
    18661 ccttcgttac cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa
    18721 tcgtattttt tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt
    18781 ttagagtcca gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg
    18841 ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca
    18901 tccccctgaa aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgctc
    18961 tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc
    19021 agcgccacgg ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc
    19081 accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca
    19141 actccgctac atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc
    19201 tgcacaagtc cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg
    19261 taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc
    19321 tgcagctgat cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga
    19381 acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta
    19441 aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg
    19501 cacaatgccg cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc
    19561 aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg
    19621 agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg
    19681 cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta
    19741 aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatggcc
    19801 tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg
    19861 agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt
    19921 tcgatggctt ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt
    19981 tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca
    20041 tcgtaggaga agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata
    20101 tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc
    20161 agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag
    20221 tcacggctgc gaagttcatc cagcatagcg ccacggatac gctacactgc gtaagttgta
    20281 aatgccgttc cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc
    20341 gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct
    20401 tcgtgacgca ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca
    20461 gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta
    20521 tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg
    20581 gttacgcaaa aagccgaatg taaaaaaccc cgccggtggc ggggtttgag aggcattcag
    20641 acgattaacc ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca
    20701 cggagttacc ggcctgctgg ataatctgcg ctttcgacat gttggacact tcggtcgcat
    20761 agtcggcgtc ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt
    20821 tggtgactgc ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga
    20881 tggagctgat ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt
    20941 cagcatctgt cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag
    21001 tcactgaacc atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt
    21061 tgttatcaac ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc
    21121 tataacctgc agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag
    21181 ctgcctgatc aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat
    21241 taccggtaaa ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg
    21301 ctgcaagtgt ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc
    21361 gcccagttgt atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc
    21421 tgatctgaac atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa
    21481 ctcccggggt caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt
    21541 atgacttact tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt
    21601 tattaatggt tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt
    21661 tagcagcatc gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag
    21721 ctaccaagtc agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa
    21781 acccattcag ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc
    21841 cgtcattcgc accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt
    21901 tgaactgggt ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct
    21961 gaatggagtc cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac
    22021 ggatacgctg taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag
    22081 aaataccgtc gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac
    22141 ggttagcaat cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag
    22201 acagacgctc gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga
    22261 tcagcgagag gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag
    22321 tcgttgatta cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt
    22381 ctgtcgtaaa acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga
    22441 aatactcttt tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt
    22501 gcgcaattca ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc
    22561 gcatggcaag tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg
    22621 atagcctcac cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt
    22681 ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg
    22741 ccaatactgc attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca
    22801 ccgcgttcag tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca
    22861 cccatctggc gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg
    22921 cgatcgccac cagcgacagc aaactcacca ttcaacaagg cgacgacaaa gatccgatta
    22981 ccattgatat cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg
    23041 caaaagcggg cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag
    23101 tcacatcaaa tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg
    23161 cgctacaaag ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg
    23221 tcgccgccca gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca
    23281 ctatcagcga cgcgctggaa aacatcactc tgaacctgaa cgatgtcacc acgggcaacc
    23341 agacgctaac catcactcag gacacctcca aagtgcaaac ggcgattaaa gactgggtga
    23401 atgcctataa ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg
    23461 cgggagctga tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga
    23521 cgattcagac gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa
    23581 cgctggcgca gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg
    23641 acaaactcac cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg
    23701 atggtaaaaa aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga
    23761 caacgggcat tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta
    23821 aagactacaa cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat
    23881 ttacccaact ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc
    23941 agttcgaaaa caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg
    24001 cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga aagcaccgta atgagcgcca
    24061 gccaacagca gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc
    24121 gactgtttat gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca
    24181 acatcattga gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc
    24241 aaaacttaat tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca
    24301 acgatgtctc agcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga
    24361 aagagtcttt actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca
    24421 tttgtatttc gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac
    24481 ggaagaacaa tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga
    24541 gatcgcacac ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc
    24601 gatgctgcac ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat
    24661 ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta
    24721 tggcgatcag ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag
    24781 ccgatggctc gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt
    24841 cgaagaatgc gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt
    24901 aagctctggc ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg
    24961 gtctggttgc cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc
    25021 tatgatttat ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt
    25081 gataaagcac aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg
    25141 ctggacgtgg tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag
    25201 cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg
    25261 acgcgttaca ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa
    25321 tgttttagcg gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt
    25381 aacgactata ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat
    25441 tatctgatgg gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac
    25501 tgggcgcgct gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa
    25561 catattccgg catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag
    25621 ccgctgttta ttgtggctga atgctggtcg catgaagtcg ataaactgca aacgtatatt
    25681 gatcaggtgg aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa
    25741 gcatcgcgca tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa
    25801 gccgatcctt ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc
    25861 ctcgaagcac cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg
    25921 gaaaatggcg ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc
    25981 ggtggtgacg ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta
    26041 attctcgccc gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac
    26101 tgcattgcct ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg
    26161 aacggagatg atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg
    26221 cgtgattttc tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc
    26281 ttcttttgca acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat
    26341 ccccggcggc aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc
    26401 cagcgcggcg gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta
    26461 ctcaatggtg ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt
    26521 gagatcatca gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg
    26581 gcaatccaag gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac
    26641 cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt
    26701 tttcacgaca ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc
    26761 tgcaattgcg agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt
    26821 aaagctctga tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt
    26881 ttgtgaatta ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca
    26941 aaccttctga gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg
    27001 attaaattct gggcacccat ccctgcggtc atcgcggcgg gtattctctc cacttactat
    27061 tttggcatta ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag
    27121 ctcctgcagc tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg
    27181 gaaggatcgc cattaacccg gatcgacggc atgatgatcc tcggtatgtt tggcggctgc
    27241 tttgctgctg cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc
    27301 attatgcagg ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc
    27361 tgtaacctgg cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt
    27421 gccatcgcca ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc
    27481 cgtattcccg tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat
    27541 caggcgcggc gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gggctgggcg
    27601 ctgctcacgg cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt
    27661 ggtttactga ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc
    27721 accggacgta cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg
    27781 atcttcagtt acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg
    27841 gtaattggtg gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc
    27901 tggatgtacc gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg
    27961 atcggctcaa cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac
    28021 tgggacaaaa tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat
    28081 ttgctgctgt ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt
    28141 gaggcaccgc agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg
    28201 atatggtggg tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat
    28261 taaaaaaagg tgagatcctg gaagtggtga gcgactgtcc gcagtcgatc aataatattc
    28321 cactggatgc gcgtaatcac ggctacacgg tgctggatat tcagcaagac gggccgacca
    28381 ttcgttattt aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg
    28441 ttacctctga cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag
    28501 gaggttgcta tgtgtggacg ctttgcccaa tcccaaacgc gtgaggatta ccttgcgctt
    28561 ctcgcggaag atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat
    28621 gtcgcgccgg gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat
    28681 ccggttttct ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc
    28741 gtagaaactg cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc
    28801 tgttttgccg atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta
    28861 tctatcgcgc tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc
    28921 gtggtgacga agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag
    28981 atattcatga ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc
    29041 aagacattgg tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc
    29101 agttcacctg gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt
    29161 taattcaacc tgtttgttga catcctccac gccctgaatg acgaggaccc ctgctacgtt
    29221 caggctgtcg cctgaatcat ttcggtgggt tcctgcttca acgagcggcc tgactgcacc
    29281 atctctccac aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc tccatttaca
    29341 tcggcgttcg ctgtatatcc acatacctgg catctgaatt tactttgtga caggcgattt
    29401 tctttcgctg tatgaccaca gcacgcgcaa cgctggcttg tgtacgctgg cggaacagca
    29461 agcacctgac cgccacgcca gagctgcttg tactcaagct ggcggcgcat ttcataccag
    29521 ccctgatcca gtatcgaacg gtttaaacct gattttgccc ggacattgcg ccccggctgg
    29581 ctgaccgtac ccgctgctga ctttgacatg ttgctgacct tcaaatcctc aatgactatc
    29641 attacgtggt ttttgctgac ggtcgttgtg actttgtgaa ggtagtccct gcggatattt
    29701 gcgatacagg aatgcagtcg ctgtattttg cgtttctgct tctgccagtt gttgctgaat
    29761 ttgaccttgc ggcttaactg tcgctgaagt ctcgccagcg tcttctggtt tttctgaaaa
    29821 ctgtttacag gctcaaagac tgtgccatct gacagcgagg caagtttagc cacgccagca
    29881 tccagcccga ccattgatgc tgaagggtga accggagttg atacttcact ttctgcctga
    29941 atactgatgt accacttacc gcaggactgg ctgacagtga catttttcac aacacccgtg
    30001 acctgacggc tgttccggta gcgcatccag ccaagttttg gcagaaaaat acggctgttt
    30061 tcctgagcga gcttaacgcc ctgcggatag cggaatgcat cattctgtca ccgctttttg
    30121 aatcagggga acgccgagcg cttctggaaa aagtttttgc aggcctgctc agggtctttc
    30181 agtgactgtt gcaatggctg tgacggagca tctttaatcc attgcgtttc agtggcattt
    30241 ttccactcaa ccaatcagga agccattttc gcgtaaggga tgtctttgtt accggcctta
    30301 tgattctcat tctgacgtgc cagagtacga ctgaaaacga aacgacatgc gcctgcgaag
    30361 cgcctcattt cacgcgcctg ttgaccaccg ggtcttaacc ggaatttgaa tacctgtagt
    30421 cgtttcacag ggcaaattat actttggtct gtgaaaaaaa ctgatatccg acgcggaagg
    30481 cactgtgttt tcctgatgca tgctcaccgg gtctttgtta ccaagtaccg acgccagatt
    30541 tttgaccatg acgcgacaga aaaactacgc acttactttt caaatgtatg tgcttatttt
    30601 gaagctgaac tggttgaaat ggatggcgaa ccagatcacg tccatctgtt aattaactat
    30661 ccccccaaac tggcgatatc cagtctggta aacaaacagc ctcaaaggcg tatcgggtag
    30721 gttactgcga cgggatcgac cagatattgc tgccaggtat taccgcaaag atgttttgtg
    30781 gcgtcccggc tattttgcca gtagctgcgg aggtgcgcca atatccgtca tccgccaata
    30841 cattgaacaa cagcaaacac ctggtcagat gaaaaaacga gccttatatc cccgccctga
    30901 aggacgggct tttacggtgc actggataag cctggagatt atctacacct gcatggacat
    30961 cattgtacga tgccccgtac atgctccatc acatcctcaa catcaaagta ttagagcctt
    31021 gcaaaccatt atgcgtctca gtttgtctca tctatcttac gcaaaaaaaa tagaactaat
    31081 atcaagcggc gctatattgc gtattaaata aaaccacctc aattacatta ataaatctaa
    31141 ataaatatca tgcaaataat ttccatggtt atttttaggc tatttaaatt aaaaaaataa
    31201 attatgagtc aatcataaat aacaaccttt aatataattt gactaccaaa tttatattcg
    31261 ttcacaaaat agtcatgcat tgtgtgcaat agaaacagtt cagataaaaa tagggatcta
    31321 cttcacaatt taaatgagaa actaaaactt acatcttgaa ataatctcat tgattggatg
    31381 aatatttgta gtgcagtgac atcattttta aataatagtt caaataaaag ggctcacgat
    31441 gaaaaaatta acagtggcaa tttctgctgt agctgcatca gtactgatgg cgatgtctgc
    31501 tcaggcagca gagatttaca ataaagacag taacaagctg gatctgtacg gaaaagttaa
    31561 tgctaagcac tacttctctt ctaacgatgc agatgatggc gacacaactt atgttcgtct
    31621 gggcttcaaa ggcgaaactc aaatcaacga tcaactgact ggtttcggtc agtgggaata
    31681 tgaattcaaa ggcaaccgtg ctgaatctca aggttcttcc aaagataaaa cccgtcttgc
    31741 cttcgctggc ctgaaattcg gtgactacgg ctccatcgat tatggccgta actacggtgt
    31801 agcatacgat atcggtgcat ggaccgacgt tctgccagaa ttcggtggcg atacctggac
    31861 ccaaacagat gtgttcatga ctggtcgcac cacaggtgtt gcaacctatc gtaacaacga
    31921 cttctttggt ctggttgatg gtctgaactt tgctgctcag tatcagggta aaaatgaccg
    31981 cactgacgta actgaagcta acggtgatgg tttcggtttc tccactactt atgagtatga
    32041 aggattcggt gtaggtgcaa cctatgctaa atctgaccgc actaatgatc aggttatcta
    32101 cggtaacaac agcctgaatg catctggtca aaatgctgaa gtatgggcag ctggtctgaa
    32161 atatgatgcg aacaacatct atctggctac cacctattct gaaacccaga acatgactgt
    32221 ttttggtaat aaccatattg ccaacaaagc acaaaacttc gaagtagttg cacaatatca
    32281 gttcgacttc ggtctgcgtc cgtccgttgc ttacctgcaa tctaaaggaa aagacttggg
    32341 tgcgtggggt gatcaggacc tgattgaata tattgatgta ggtgcaacct attacttcaa
    32401 caaaaatatg tccacttttg ttgattacaa aatcaacctg attgataaga gcgatttcac
    32461 gaaagcatct ggcgttgcta ccgatgatat cgttgctgta ggattggttt accagttcta
    32521 atctgattac tcaaagatat gttgtgggag gctttgcctc cccaacatat aagtggcgct
    32581 ctcaagccac tttccttaga agcactatct tgcttctgac tatataaatt accctttatt
    32641 ttctgggacg tttcaacgcc ccatttttaa taatactcag tgaacaatta acgtatcaat
    32701 tagatttaat aacaacgata tccgtaccta acctgatatc taatgtcata agcatccctt
    32761 taattatccc ctcaacctct tgtaaatttc ccactcttga gctccacata gcccaaacgc
    32821 cggatgcaaa aaaaatcata cgtgcatctc tgaaattgtg atggaggaag tgagtatcaa
    32881 ttatatgtgc ttataaacgt tcccgattgg cacagaagtc accggattgt tcaggctcca
    32941 ataggttgag tatgtcgtat ttattttcca aaataaaaat cgatgctgtt gctccttaat
    33001 aaaatacttt tactctctgg acgcttttct tagctctctt ggtgttatat taaaacgatt
    33061 atggaacctt tcagtaaaac gagaaggaca cttgtaacca ttttccctgg caatctcgct
    33121 gatgggtttt actgtcgttt gtatagcagg acaacgcatt atttaacctc acgtcgtcca
    33181 atatactttg aaaacttatt ccctcgcttg ccagacggcg atgcaatgtg gaaacagaaa
    33241 tgtagagata tctcgctacc ttgtttgctg tccattttgt tctaggttcg gatagcatta
    33301 ggttataaca acgacttatt aatgattgtt tactatctga taaaagtaaa caattaacat
    33361 gatgtgctcc taatgaaagt agaacgccca ttgctaagtg ttcctgaatt tgcgttgaga
    33421 agcctcggga aatagatgtt tttagttgct cccaacaata tattaactcg gggttctgag
    33481 gtaagcagaa acctgtcgtg ttacgaattt gatcagttat cgcataaaat ttttgaaact
    33541 tttcaattag atcaatggga taataaagca tttctgcaag ataaagacct gcttctggat
    33601 aattcacaat ataaaattca tatccacagg gaaataatat tatctgatta ttatcaacag
    33661 ttagactatg cgtctcccaa ttgataactt tttttccctg acggatacga cataaagctg
    33721 gcataagagg cttaaccctg tgaatctcat gatgtttatg catccgtatt tcttcaatct
    33781 ttaggttaga cttatctctt gccagcatac tcttacccta ttttatctca taaactggtg
    33841 ttatctcagc ggttgcaatt ttattggcat taagcatata accaactaac gctccgctgg
    33901 aattagaatc cacaggaatc ttatcagttt ttagagccca tactttaaac tggtaatgat
    33961 ggggtttatc tcctttggga ggacatgctc caccaaatcc tgcataacca aaatcatttc
    34021 ggccttgaac agcaccagtc ggaagttttg taccatcacg tcttcctgca tcagtgggca
    34081 aatatgttac agttgctgga atattagcaa cagtccagtg ccaccatcca ctgcctgtag
    34141 gagcatctgg atcatacaca gttacggcaa agcttttggt gccttcagga gcaccagacc
    34201 aggttaatga gggcgatgta ttaccacctt cacacccaaa tccagaaaat acatgagacg
    34261 ttgtaagttg ctctcctgtt tttatttcat tactagtgac ctgaaatgct gcggcctgcg
    34321 cagaaaatgt tatgaatgcc aatacacttg aaacgataag ttttttcata aaaacctcat
    34381 tgttgtgacc tatcgttatt ttatttgatg tgccttgatc tcattatgca taaaggcgca
    34441 atgatcgcgc aaatgcaatc ataattataa aggtgccccg aacattagcc ataatatgca
    34501 caaatcagat tcctgttatc tcaaggagaa ttatttttta atgcggtgca cttcgtgaca
    34561 acaagttaat gactaacaca ccggcacaaa tcaacatcat gcctatgata gctggcaggt
    34621 ccagtcgttg accaaaaaat ccccaggaaa gcaaactaat cagaacgata ccgactcctg
    34681 accagatagc ataagcaatc cctgtaggta tataagccag cgtctgagct aataaccaga
    34741 atgatgcaca atagcaaata attgtgccaa cagagggcca taaccgtgta aacccctctg
    34801 aaaacttcat taaggttgta ccaatgacct ctgcaagtat tgcgccacca agataaatat
    34861 aagggttcat agcatattct ttcctgttca tactggagag aattgtacta cagtttgaac
    34921 acaactcacc gttttcatcc tggtgtatgt gtatccaagg ggctctggcg tggcaagaga
    34981 taaaggcagg aacaataatg atcagaagga accccaggct aacgaatgag ttcactcttc
    35041 ctgggagcaa agcgaagaca aaacatgtta tcgcgtttac ggatcgtgga tggcagaaaa
    35101 caaccaggat caggtatcca ttttaaacca gaaattgagt gagtttgccc catccatgcc
    35161 ccacgctgtg ggatcggata taattaaaca agcatagtca acaggttaca caacctacac
    35221 ctgcatggac atcacttcct gatacgccgc caccagctta ttacgcacct gaatccccat
    35281 ttgcatagaa actgaggctt tttgcatatc ggtcatcaca tcgtttagcg ccacgccggg
    35341 ttcaccgagg gtgaattttt ctgcctgcgt gcgggcgact gtttgtgtat cgcttatgcg
    35401 atcgagcgcg gcgtgcagct gcccggcaaa actaatggtc ggttgcggca gtgattcctg
    35461 cgcacgcgca ctcatcgccg tagcctgtaa ctggctgata accccttcaa tcccctgtat
    35521 cgctgacatt ttcgtctccc ggataatttc tggtaacaaa gcctaccagt aagtcaataa
    35581 gacaaaggcg ctaaatagca acaaaaaaac tggtttattg gcggatagaa aaaacgaaag
    35641 cacaaataat gggagcgtca atttttcgag tgtgctgacc cgagagtgag tcttgttcca
    35701 ctttgccaat aacgccgtcc ataatcagcc acgaagtgcg cgatgaatgc gactgcagcc
    35761 cagacaaaat ctcttgagtg gcttaatcgc ctgcgtgcga atccgaaaat tccattgatt
    35821 gttaccggtt ccgcggcagt ggcggtcatg gtcgcactga tcctgtgggc gaaagccccc
    35881 gactaccgca cattattcag caatctttcc gatcaggatg gtggcgcaat tgtcagccaa
    35941 ctgacgcaaa tgaatattcc ttaccgcttc agcgaagcca gcggcgctat tgaggttccg
    36001 gcagataaag ttcacgaact gcgcctggcg caacaaggtt tgcccaaagg cggcgcggtc
    36061 ggtttcgaac tgctcgatca ggaaaagttt ggtatcagcc agttcagcga acaggtgaat
    36121 tatcagcggg cgctggaagg cgagctttct cgtaccatcg aaactatcgg cccggtaaaa
    36181 ggggcgcgcg tacatctggc aatgccgaaa ccgtctttat tcgtccgtga acaaaaatcc
    36241 ccttctgcat cggtgacggt aaatctgtta cccggccgcg cactcgatga agggcaaatt
    36301 agcgctattg tacatctggt ttccagcgcg gttgctggtc tgccgcctgg aaacgtcacg
    36361 ctggtggatc agggcgggca tctgttaacc cagtccaata ccagcgggcg cgatcttaat
    36421 gacgctcagt tgaaatatgc cagcgatgtc gaaggccgta ttcagcagcg tattgaagcg
    36481 atcctgtcac ctattgttgg taacggtaat attcacgcct aggtcacggc gcaactggac
    36541 ttcgccagta aagaacaaac ggaagaacag tatcgcccta acggtgatga atctcatgcg
    36601 gcgcttcgtt cacgccagct taatgagagc gagcaaagcg gttccggtta tccgggcggc
    36661 gtaccggggg cgttgtcgaa tcaaccggca cctgcgaata acgcgccaat cagcacgcct
    36721 ccgacaaatc aaaataaccg ccagcagcag gcgagcacca ccagcaatag tgggccgcgt
    36781 agcacacagc ggaatgaaac cagtaactac gaagtcgatc gcaccattcg tcataccaaa
    36841 atgaacgtgg gcgatgtgca acgtctgtca gtcgcggtag tggtgaatta caaaaccttg
    36901 ccagacggta aaccattgcc tctcagcaac gaacagatga agcaaattga agctctgacc
    36961 cgcgaggcga tgggcttttc tgaaaaacgc ggtgattcgc tcaatgttgt taactcgccg
    37021 ttcaatagca gtgacgaaag cggcggagca ctgccgtttt ggcaacagca agtgtttatc
    37081 gatcagttgc tggctgccgg tcgctggttg ctggttgctg gtactgctgg tggcgtggtt
    37141 gctgtggcgg aaagcggtac gcccgcagct aacacgtcgc gccgaggcgg tgaagactgt
    37201 gcagcaacag gcgcaggccc gcgaggaagt ggaagatgcg gtggaagtcc gtctgagcaa
    37261 agacgaacaa ctccaacaac ggcgcgctaa ccaacgactg ggggcagaag tcatgagcca
    37321 gcgtatccgt gaaatgtctg ataacgatcc gcgcgtggtg gcgctggtca ttcgccagtg
    37381 gataaataac gatcatgagt aacctgacag gcaccgataa aagcgtcatc ctgctgatga
    37441 ccattggcga agaccgggcg gcagaggtgt tcaagcacct ctcccagcgc gaagtgcaaa
    37501 ccctgagcgc tgcaatggcg aacgtcacgc agatctccaa caagcagcta accgatgtgc
    37561 tggcggagtt tgagcaagaa gctgaacagt ttgctgcact gaatatcaac gccaacgatt
    37621 atctgcgttc ggtattggtc aaagctctgg gtgaagaacg tgccgccagc ctgctggaag
    37681 atattctcga aacccgcgat accgccagcg gtattgaaac gctcaacttt atggagccgc
    37741 agagcgccgc cgatctgatt cgcgatgagc atccgcaaat tatcgccacc attctggtgc
    37801 atctgaggcg cgcccaggcc gccgatattc tggcgttgtt cgatgaacgt ctgcgccacg
    37861 acgtgatgtt gcgtatcgcc acctttggcg gcgtgcagcc agccgcgctg gccgaactga
    37921 ccgaagtact gaatggcttg ctcgacggtc agaatctcaa gcgcagcaaa atgggcggcg
    37981 tgagaacggc agccgaaatt atcaacctga tgaaaacgca gcaggaagaa gccgttatta
    38041 ccgccgtgcg tgaattcgac ggcgagctgg cgcagaaaat catcgacgag atgttcctgt
    38101 tcgagaatct ggtggatgtc gatgatcgca gcattcagcg tctgttgcag gaagtggatt
    38161 ccgaatcgct gctgatcgcg ctgaaagggg ccgagcagcc actgcgcgag aaattcctgc
    38221 gcaatatgtc gcagcgtgcc gccgatattc tgcgcgacga tctcgccaag cgtggtccgg
    38281 tgcgtctgtc gcaggtcgaa aacgaacaga aagcgattct gctgattgtg cgccgtcttg
    38341 ccgaaactgg cgagatggtg attggcagcg gcgaggatac ctatgtctga taatctgccg
    38401 tggaaaacct ggacgccgga cgatctcgcg ccaccaccgg cagagtttgt gccaatggtc
    38461 gagtcggaag aaaccattat tgaagaggcc gaacccagcc ttgagcagca actggcgcaa
    38521 ctgcaaatgc aggcccacga gcagggttat caggcgggga ttgccgaagg tcgccagcaa
    38581 ggtcacgaac agggctatca ggaaggactg gcccaggggc tggaacaagg tctggcagag
    38641 gcgaaggctc aacaagcgcc aattcatgcc cggatgcagc aactggtcag cgaatttcaa
    38701 actacccttg atgcgcttga tagtgtgata gcttcgcgcc tgatgcagat ggcgctggag
    38761 gcagcacgtc aggtgatcgg tcagacacca acggtggata actcggcact gatcaaacag
    38821 atccaacagt tgttgcagca agaaccgtta ttcagcggga aaccacagct gcgcgtacac
    38881 ccggatgatc tgcaacgtgt ggatgatatg ctcggcgcaa ctttaagttt gcatggctgg
    38941 cgcttgcggg gcgatcccac cctccaccct ggcggatgta aagtctccgc cgatgaaggc
    39001 gatctcgacg ccagtgtcgc cactcgctgg caagaactct gccgtctggc agcaccagga
    39061 gtggtgtaat gaccacgcgc ctgactcgct ggctaaccac gctggataac tttgaagcca
    39121 aaatggcgca gttgcctgcg gtacgtcgct acgggcgatt aacccgcgct accgggctgg
    39181 tgctggaagc caccggatta caattgccgc tcggcgcaac ctgtgtcatt gagcgccaga
    39241 acggcagcga aacgcacgaa gtagaaagcg aagtcgttgg ctttaacggt caacggctgt
    39301 ttttaatgcc gctggaggaa gtggaaggtg tcctgcccgg cgcgcgtgta tatgccaaaa
    39361 acatttcggc agaagggctg caaagcggca agcagttgcc gctcggtccg gcgttattag
    39421 gtcgtgttct ggacggtagc ggtaaaccgc tcgatggcct gcccttcccc gatacgacgg
    39481 aaaccggtgc gctgattacc ccgccattta acccgttgca acgtacaccg attgaacatg
    39541 tgctggacac cggcgtgcgc ccaatcaatg ccctgcttac cgtcggacgc gggcagcgta
    39601 tggggctgtt tgccgggtcg ggcgttggta aaagtgtgct gctggggatg atggcacgct
    39661 acacccgcgc cgatgtcatt gtcgtgggtt tgattggtga acgtgggcgc gaagtaaaag
    39721 attttattga gaatatcctc ggtgccgaag gacgtgcacg ctcagtggtg attgccgctc
    39781 cggcggatgt ttctccgctc ctgcgaatgc aaggtgccgc ctatgccacg cgcattgccg
    39841 aagattttcg cgatcgtggt cagcatgtgt tgctgattat ggactccctc acccgctacg
    39901 cgatggccca gcgtgaaatt gcgctggcga ttggcgaacc acctgccact aaaggttatc
    39961 caccgtcggt gtttgccaaa ttaccggcac tggtcgagcg tgccggtaat ggcattagcg
    40021 gcggcggctc gattaccgcg ttttataccg tgctcaccga aggcgatgac cagcaggacc
    40081 ccattgctga ctccgcgcgg gcaatcctcg acggtcacat tgtgctgtct cgccgactgg
    40141 cggaagccgg gcactatccg gctatcgata ttgaagcgtc gatcagtcgc gcaatgacgg
    40201 cgttgatcag tgagcaacat tacgcgcgag tgcgcacctt caaacagctg ttgtcgagtt
    40261 ttcagcgtaa ccgcgatctg gttagcgtcg gcgcgtatgc caaaggcagc gatccgatgc
    40321 tcgataaagc catcgccctg tggccgcagc tggagggcta tttgcaacaa ggcatttttg
    40381 aacgcgcgga ctgggaagcg tctctccagg ggctggagcg tattttcccg acagtgtcat
    40441 aacccaggag ataacggcag atggcggaag aacagctcaa aatgttgatc gattatcaga
    40501 atgaatatcg caataacctc aatagcgata tgagtgccgg gatgaccagc aaccgctgga
    40561 tcaactatca gcagtttatc cagacgctgg aaaaagccat tactcagcat cgccagcaac
    40621 ttaatcagtg gacgcagaaa gttgacattg ccctgaacag ctggcgagaa aagaaacaac
    40681 gtttgcaggc ctggcagaca ctgcaggaac ggcaatccac ggcggcactg cttgcagaaa
    40741 accgcctcga tcagaaaaag atggatgagt tcgcccagcg cgccgccatg aggaaacctg
    40801 aatgattcgc ttagcgccct tgattaccgc cgacgttgac accaccacat tgcctggcgg
    40861 caaagccagc gatgctgcac aagattttct cacgttgttg agcgaagcat tagcaggcga
    40921 gacaacaact gacaaagcgg ccccccagtt gctggtagca acagataagc ccacgacaaa
    40981 aggcgagccg ctggtcagcg atattcttgc cgatgcgcaa caagcggatt tactgatccc
    41041 tgtggatgaa acacttcctg tcatcaacga cgaacaatcc acatcaacac cgttaaccac
    41101 cgctcagacg atgacgttgg ctgcggtggc tgacaaaaat acgacaaaag acgaaaaagc
    41161 ggatgatctt aatgaagacg tcaccgcaag tctgagcgcc ctttttgcga tgttgccggg
    41221 ttttgacaat acgcccaaag tgactgatgc accgtcaacc gtgttaccgg cagagaaacc
    41281 aacgctcttc acaaaactga cttctgcgca actcactaca gcacagcctg atgacgcccc
    41341 cggcacacca gctcagccat taacaccgct ggtagcagaa gcccagagta aagcggaagt
    41401 catcagcacg ccttcgccgg tgaccgctga cgccagcccg ctaatcactc cacaccagac
    41461 acagccactg cccaccgtcg ccgcgcctgt tttgagtgca ccgctgggtt ctcacgaatg
    41521 gcaacaatca ttgagccagc atatttcgct gttcacccgc caggggcaac aaagtgcaga
    41581 gttgcgtctg cacccacagg atttaggtga agtgcaaatt tccctcaaag tggatgataa
    41641 ccaggcgcag atccagatga tttcaccgca tcagcacgta cgcgccgccc tggaagcagc
    41701 gctgccggta ctgcgtacgc agctggccga aagtggcatt cagttagggc aaagcaacat
    41761 tagtggcgaa agctttagtg gtcagcagca ggccgcttcc cagcaacagc aaagccaacg
    41821 cacagcaaac catgaacctc tggcggggga agacgacgat acgcttccgg ttcccgtctc
    41881 tttacaaggg cgtgtaacag gtaacagcgg cgttgatatt ttcgcctaac gtcagaggta
    41941 gcaccgtaat ccgcgtcttt tccccgcttt gttgcgctca agacgcagga taattagccg
    42001 ataagcagta gcgacacagg aagaccgcaa cacatgactg attacgcgat aagcaagaaa
    42061 agcaagcgat cgctttggat cccgattctg gtattcatta ccctcgcggc ctgtgccagc
    42121 gcaggttaca gctactggca ttcgcatcag gttgccgctg acgacaaagc gcagcaacgc
    42181 gtcgtgccct ccccggtctt ctatgcgctg gataccttta cggtcaattt gggcgatgcg
    42241 gatcgcgtgc tttatatcgg cataaccctg cgactgaaag atgaagcaac ccgctcgcgg
    42301 ctgagtgagt atttgccgga agtccgtagt cgcttgctgt tactgttttc acgtcaggat
    42361 gctgccgtac tggcgacaga agaaggcaag aaaaacctga ttgccgagat taaaaccaca
    42421 ctttccaccc cgcttgttgc cgggcaaccg aaacaggatg tcaccgacgt gctgtatacc
    42481 gcttttattc tgcgataacg acatgggcga tagtattctt tctcaagctg aaattgatgc
    42541 gctgttgaat ggtgacagcg aagtcaaaga cgaaccgaca gccagtatta gcggcgaaag
    42601 tgacattcgt ccgtacgatc cgaataccca acgacgggtt gtgcgcgaac gtttgcaggc
    42661 gctggaaatc attaatgagc gctttgcccg ccattttcgt atggggctgt tcaacctgct
    42721 gcgccgtagc ccggacatta ccgtcggggc catccgcatt cagccgtacc atgaatttgc
    42781 ccgcaacctg ccggtgccga ccaacctgaa cctaatccac ctgaaaccgc tacgcggcac
    42841 agggctggta gtgttctcgc cgagtctggt gtttatcgcc gtggataacc tgtttggcgg
    42901 cgatggacgc ttcccgacca aagtggaagg tcgcgagttt acccatactg aacagcgcgt
    42961 catcaaccgt atgttgaagc tggcgctgga aggctatagc gacgcctgga aagcgattaa
    43021 tccgctggaa gttgagtacg tgcgttcgga aatgcaggtg gaatttacca atatcaccac
    43081 ctcgccgaac gacattgtgg ttaacacgcc gttccatgtg gagattggca acctgaccgg
    43141 cgaatttaat atctgcctgc cattcagcat gatcgagccg ctacgggaat tgttggttaa
    43201 cccgccgctg gaaaactcgc gtaatgaaga tcagaactgg cgcgataacc tggtgcgcca
    43261 ggtgcagcat tcacagctgg aactggtggc caactttgcc gatatctcgc tacgcctgtc
    43321 gcagatttta aaactgaaac ccggcgacgt cctgccgata gaaaaacccg atcgcatcat
    43381 cgcccatgtt gacggcgtcc cggttctgac cagtcagtac ggtactctca acggtcagta
    43441 tgcgttacgg atagaacatt tgattaaccc gattttaaat tctctgaacg aggaacagcc
    43501 caaatgagtg acatgaataa tccggccgat gacaacaacg gcgcaatgga cgatctgtgg
    43561 gctgaagcgt tgagcgagca aaaatcaacc agcgaaaaaa gcgctgccga cgcggtgttc
    43621 cagcaatttg gcggtggtga tgtcagcgga acgttgcagg atatcgacct gattatggat
    43681 attccggtca agctgaccgt cgagctgggc cgtacgcgga tgaccatcaa agagctgttg
    43741 cgtctgacgc aagggtcagt cgtggcgctg gacggtctgg cgggcgaacc gctggatatt
    43801 ctgatcaacg gttatttaat cgcccagggc gaagtggtgg ttgttgccga taaatatggc
    43861 gtgcggatca ccgatatcat taccccgtcc gagcggatgc gccgcctgag ccgttagtga
    43921 tgaataacca tgctactgtg caatcttccg cgccggtttc tgctgagcca ctgctgcagg
    43981 tgagcggcgc actcatcgcc attattgccc tgatcctcgc tgctgcctgg ctggtaaaac
    44041 ggctgggatt tgcccctaaa cgcactggcg ttaacggtct gaaaattagc gccagtgctt
    44101 cgctgggcgc gcgtgaacgg gtcgtggtgg tcgatgtgga agatgcacgg ctggtgctcg
    44161 gcgttaccgc agggcaaatc aatctgctgc ataaacttcc cccttcagca ccaacggaag
    44221 agataccgca gaccgatttt cagtcggtca tgaaaaattt gcttaagcgt ggcgggagat
    44281 cctgatgcgt cgtttgtttt ctgtcgcacc ggtccttctc tggctgatta cgcccctcgc
    44341 cttcgcgcaa ctgccgggta tcaccagcca gccgctacct ggcggtggac aaagctggtc
    44401 gctcccggtg cagacgctgg tgttcatcac ctcgttgacg ttcattccag cgattttact
    44461 gatgatgacc agtttcaccc gcatcattat tgtttttggt ttattgcgta acgcgctggg
    44521 aacgccctcc gcgccaccta accaggtatt gctggggctg gcactgtttt tgaccttttt
    44581 tattatgtcg ccagtgatcg acaaaattta tgtcgatgcg taccagccat tcagcgaaga
    44641 gaaaatctca atgcaggagg cgctggaaaa aggggcgcag ccgctgcgtg agtttatgct
    44701 gcgtcagacc cgcgaggcag atttagggtt gtttgccaga ctggcgaata ccggcccgtt
    44761 gcaggggcct gaagctgtgc cgatgcgcat tttgctcccg gcctacgtga ccagcgagtt
    44821 gaaaaccgca tttcagatag gcttcacgat tttcatccct tttttgatta tcgacctggt
    44881 gattgccagc gtgttgatgg cattggggat gatgatggtt ccccccgcca ccattgctct
    44941 gccctttaaa ctgatgctgt ttgtactggt ggatggctgg caattgctgg tcggttcgct
    45001 ggcgcagagc ttttacagct agagaggcaa aatgacacct gaatcggtca tgatgatggg
    45061 aactgaagcg atgaaagtcg cgctggcact ggcagccccg ctgttgctgg tagcgctggt
    45121 cacgggcctt atcatcagta ttttgcaggc agccacgcag attaacgaaa tgacgctgtc
    45181 gtttattccg aaaatcatcg ccgtatttat cgccatcatt attgccggac cgtggatgct
    45241 caatctgttg ctggattacg tccgcacctt gttcactaac ctgccgtata tcatcgggta
    45301 gcccgtacaa tgatgcagga gacaagcgat caatggcttt cctggttaag cctgtacttc
    45361 tggccgttac tgcgcgtgct ggcgctcatt tctaccgcgc caattctgag cgaacgcagc
    45421 gtgccgaaac gggtaaaact gggtctggca atgatgatca cgttcgccat tgccccatca
    45481 ttacctgcca acgatgttcc tgttttttcg ttctttgctc tgtggctggc cgtacagcag
    45541 atcctgatcg gcattgcgct tggttttacc atgcaatttg cctttgccgc tgtgcgaacc
    45601 gctggcgaaa ttatcggtct gcaaatgggg ctgtcatttg cgacatttgt cgatcctggc
    45661 agccatctca atatgcccgt tttagcgcgc atcatggata tgctggcatt actgctgttc
    45721 ctgacattta acggtcattt atggttgatt tcactgctgg tcgatacctt tcacaccctg
    45781 ccgattggta gcgaaccgtt gaacagcaat gcgtttctgg cacccaccaa agcagggagt
    45841 ttgattttcc ttaacgggct gatgctggcg ttaccgctca ttactctgtt gctgaccctg
    45901 aatctggcat taggtttact gaatcgtatg gccccgcaat tatccatttt tgttattgga
    45961 tttccattaa ctctgactgt cggcatctct ttaatggcgg cattaatgcc gttaattgcg
    46021 cctttttgcg aacatttatt cagtgaaatt tttaatttgc tggctgatat tattagtgaa
    46081 ttaccattaa tataattccg taacgtttat catgctaccc taaggattat ccgaaaaata
    46141 atatctgcga acatcttcca ggatactcct gtagcgaaat atttgtttta agctcactca
    46201 catatcgcaa catttacttt aagacaattc caggcaaatt atacaacact ttacgggata
    46261 gtaagtccgc ctgaaaaatt gcgagagtgg cgcattaggt gacccatgtt gttccgttta
    46321 gtcatgatga aatattcagg taaggggaat tatcgttacg cattgagtga gggtatgcca
    46381 tgtcaacgat tattatggat ttatgtagtt acacccgact aggtttaacc gggtatctgt
    46441 tgagtagagg ggttaaaaaa agagaaatca acgacattga aaccgttgat gaccttgcca
    46501 tagcttgtga ttcacagcgt ccttcagtgg tgtttattaa tgaggactgt ttcatccacg
    46561 atgcttctaa cagtcagcgc atcaagcaca tcattaatca acatcccaat acgttattta
    46621 tcgtttttat ggcaattgcc aatgttcatt ttgatgaata tctattggtc agaaaaaatt
    46681 tattgatcag ttctaaatcg attaaaccgg aatctctcga cgatatcctt ggcgatattc
    46741 tgaaaaaaga gacaacgata acctcgtttt taaatatgcc gacgttatca ttgagccgaa
    46801 ccgaatcgag tatgttgcga atgtggatgg caggtcaggg aaccattcaa atctccgacc
    46861 aaatgaatat caaagccaag accgtttcat cgcataaagg taatattaaa cgtaagatca
    46921 aaacgcataa taaacaggtt atctaccatg tcgtccgact gacggataat gtgactaatg
    46981 gtatttttgt caacatgcgc taacacattc tgactggtgg tttcccacca gtcaggctga
    47041 ataagattac tctgctttct caacaaagat accgtcctga tgccctgctt cattaaagaa
    47101 ccagatgccg agcgggtagt cttccagcgc aaccaggtac attgtgcctt cactaaactc
    47161 ctcaactgcc agtaccactc caggacgacg cggaccgcca tccgttttga ctgttacccg
    47221 atcattcacc ttcatcgttt tcctcctgtg gctttgtgcc agtgtagaac aatttcgttt
    47281 ttctggcagc gccaagcgcg cgcgagtgct gattttctcg acggtctata cttaagagat
    47341 gccaggcgga cttaacgact ggcggcaaca acagagtaac ggttgcgagg aaagatgatg
    47401 aaaaccgcaa aagtgtacag cgataccgca aaacgtgagg tcagcgtcga tgtcgatgcc
    47461 ctgctggcgg cgatcaatga aattagcgaa agcgaagttc atcgcagcca gaacgattct
    47521 gaacacgtta gcgtcgatgg acgtgaatat catacatggc gtgaattggc ggatgccttc
    47581 gaactggata ttcatgactt cagcgtctct gaagtgaatc gttgaatgca caataaaaaa
    47641 atcccgaccc tgagggtgtc gggatgaaac ttgcttaagc aagaagcact taaaaaattc
    47701 gttacaccag gaaatctgat gtgttcatca ccttatctgc aatttttttc gctgacaaga
    47761 aaatattcgc gctatgaatg attaacgcta cttatgaata gaaatgtgac gcgcgtcacc
    47821 ccttggtggc tctggtgtca acgggatcgc tcccgaaaaa atcatctttg attatttgtg
    47881 ctgttttgag aatcatcctg gtaaggggag tactgcgcga ccatgttttc aattcaacaa
    47941 ccactactgg tttttagcga tcttgatggc accctgctgg acagtcatag ttatgactgg
    48001 caaccggcag ccccctggct cagccgttta cacgaagcca atattcccgt cattctctgt
    48061 agcagtaaaa ctttagcgga aatgctgtac ttgcaaaaaa tgttggggct acaaggctta
    48121 ccgctgattg cagagaatgg cgcagtgatc cagcttgctg aacaatggca ggatatagac
    48181 ggttttccac gcatcatctc aggtattagc catggcgaaa tctgccaggt tttaaatacg
    48241 ctacgcgaga aagaacattt taaattcacg acttttgatg atgtcgacga tgcaaccatt
    48301 gccgaatgga cgggattaag ccgtagctag gcggcgctga cgcagctgca tgaggcgtcg
    48361 gtaacgctaa tctggcgcga cagtgacgag cgtatggcac aatttatcgc tcgtctgaac
    48421 gaactgggct tgcagtttat gcagggtgcg cgcttctggc acgtactgga tgcctctgcc
    48481 ggaaaagatc aggctgccaa ctggattatc gcgacctatc aacaattgtc aggcagacgc
    48541 ccaaccacac ttggcctggg cgatgggcca aacgatgcgc ctttactgga ggtaatggat
    48601 tacgcggtga ttgtgaaagg gctaaaccgt gaaggggtac atctgcatga tgaggatccg
    48661 gcccgcgtct ggcgaacgca gcgtgaagga ccggaaggct ggcgtgaagg gctggaccat
    48721 tttttctccg cccgttaagc gttatcgctc gcgaataccc gattgcgccc ggcctgtttt
    48781 gcgaggtaaa gccgacggtc ggccaatgac tggagttgtt caaaatcata atcaccggtt
    48841 tcctcgctgc tacttacccc cagcgaggca ctgatgcgta tcgttgtact cttggcgatc
    48901 agcatctctt tttcatttaa cttcaggcga atacgttctg cgacctccgc agcctgcgtc
    48961 agattcgcgc ctggcagaat cacacaaaac tcctcaccac cgacccgccc ggcaacgtcc
    49021 tgcgcacgca aggaactgct aattaatccg gcagcatgag aaagaacacg gtcgcccgcc
    49081 tgatgaccaa agcggtcatt aatcgcttta aaatggtcaa gatcgacctg aatgacagaa
    49141 aaaggatgtt ggtgcgtctg acacaattta gcgagcggac gggctttttc gaacagtgcg
    49201 ccacggttat ataaacgcgt taaggtgtcg tgccacgcct gccactgcaa cgagctttgc
    49261 agaacataca tgttgctgac catccggcga atcacatacc aggagaggag taacatggtg
    49321 gtaaagagcg cccacagcag ggttaatgca atgctgatac tgccgaaatc gccgcgcacg
    49381 ccttcgctta gcgtatggac gcgcgccagc acaccgtcga aatggtccag acgttcccag
    49441 ctaacatagc gactgtccat acgaatgccg ccccgcgtgt catgttccat tgcctgcgcc
    49501 agcaaggcca gttcacgagg atcaaaaata ttccctgttg gatggtcagg attggaagag
    49561 gtcaaaaatc tcagcttact gtcatagagc tgatactcac catcgaggtt tttatcgatg
    49621 gcgtttctta aaaattgctg catagtacgc acgggaatac tcatccccag cacgccatac
    49681 cagtagttat tactgtctac cggaacactg acggtaacct gcggttcagt attgctggcg
    49741 tgttccggtt gcgaagtgaa ccagcgtacc gcgcggtgac gattttctcg ttgcgaatgg
    49801 ccgataaacc agggttgggt gacatagccg taataacgcg ttggtacatt gcgcgtaaac
    49861 aaggtcggct gcgtcgaaac gtaaaatcca gcacgcgaga catacatcgc ctgttcaacc
    49921 atcgacgagg agttgtgcgc cagtcgcagc aagtaaccaa cttccagtgc agcggtaatt
    49981 tcattgtcga ggctttcatt ttcgcgagac aggaaattcc cctcgctgac taatgcatcc
    50041 gatacgccat tgactggcag ggtgcgccgt cggttgagtt cgatttgcca ggcgtgctcg
    50101 tcgcgatgct gttcgaactc ggttacggca ttacgcagag aggtgaaatc cagtggcgcc
    50161 acgagagctt cgcgcatgcc attacgcaaa aagatcagtt tgtcgacgtt atactgcaaa
    50221 tgcttatcga gcgcgttggc tacgttttcc agatgattac gctagctgga gatataggca
    50281 tcttccagca ccaccacttc acgccaggtg agcaaggtgg aaaaaagcaa taccacgata
    50341 aagcagagat taacgacatg accgggcccc aggcggcgtg cgagtttttt caaccagctc
    50401 tggttttcca tttttgtctc gtgctgcacc ctgacgactc ccaacctgct tttttatgat
    50461 tctggcataa cccggcaata gcgtaccgca aaacagcgat aattttataa tggtggcatg
    50521 ataagacgcg actatcattc attgcaggca ttgggacacc aggccggatg tgatccggcc
    50581 tggtgttaat gatacccttt caaacttctt ccgcagaacg actcccctcc cccggaacaa
    50641 gatcggtttc atgaaaagct tcccgcttga ccccgtaacc gtcataccaa cgacactcaa
    50701 ccataccgct ggagtatcca gtgacaatca tccgcgggcc gccctcttta accgtaactt
    50761 cctcactaac cataaagctc atactcgcct ccttttttcg agtgaaactt gttcacctta
    50821 gttgaagatg gcgaattttg cataatagcc agtgcgagta ttaatgtgcc tgaccgcgca
    50881 ttttcgctac tgctttcact cccagcacca cgatgccgcc gatgataaat ccaagaatca
    50941 gatttaaaac agtcggtaat atcatcgcca ccactgcact ttgctgcccg gcgatatgtt
    51001 caatagcgtg atgcagcggc gcaataccat gtaccacaat cccgccgccg acgaggaaca
    51061 tcgccagcgt gccgacaatc gataatgctt tcatcagcca aggcgcgata atcaataacc
    51121 ctttacctaa tgcctgcatc agcgcgctag atttttccgc cagccaatac cctaggtcat
    51181 caatcttaac gataacccct accagaccgt agacgcccac ggtcactacc agcgcgatgc
    51241 ctgaaagcac cagcacctga ttaagcaacg gcgcctctgc cacaatcccc agcgtgatgg
    51301 cgacgatttc cgcagacaaa ataaaatcgg tacgaatcgc ccctttaatt ttgtcctttt
    51361 caaacttcag cggatcctgc gccgccagct tctccagacg ctgctggctc tgcgctggat
    51421 cttctttatg tttacgcgcc tccagcatat gcagcacttt ctctacccct tcaaagcaga
    51481 gaaacgcgcc accaatcatc aacagtggcg taatcgccca cgggataaac gcactgatga
    51541 tcagcgccag cggcaccaga atcactttat tgatcagcga tcctttcgcc acgccccaga
    51601 ccacgggaag ttcccggttg gcccgcacgc ctgaaacttg ttgcgcattg agcgataaat
    51661 catcccctaa tacaccggcg gttttcttcg ccgccagttt gcccatcaca gagatatcgt
    51721 ccagcagtgt ggcgatatca tcgagcaacg tcagtaaact actcccggcc aaaagcattc
    51781 cctctcattt ttattgttga agggtgtaag tatgaagcaa aaacggcagc tctgatactt
    51841 gcttcacagg tcaacattta tttaacaatc aagtgcaatt atatctctcg ccagcagaat
    51901 gattttcacg tttactatga accacctttt tatttccgcc gtgagggatt atgcgtttcc
    51961 gccagttgtt accgcttttt ggcgcgctgt ttgcgttgta tatcatttgg ggctcaacct
    52021 attttgtcat tcggattggc gtgggaagct ggcctccgtt aatgatggcg ggcgttcgat
    52081 tccaggcagc cggtatttta ttgctggcat ttttgctact gcgcggacac aaacttcccc
    52141 cgctacgtcc gctgctcaat gccgcgctga ttggcctgtt attgctggct gtcggtaatg
    52201 gcatggtgac ggttgccgaa catcaaaatg ttccttccgg catcgccgcc gtagtgattg
    52261 caaccgtgcc cctctttacc ctgtgcttca gtcgcctgtt tggcattaaa acgcgcaaac
    52321 tggaatgggt gggtattgcc attgggcttg ccggaatcat catgctcaat agcggtggaa
    52381 atttaagcgg caatccgtgg ggcgcgattc tgattttaat cggctcgatt agctgggcgt
    52441 ttggctcagt ttatggctcg cgcattacct tacctgtagg gatgatggcg ggtgcgattg
    52501 agatgctggc ggcaggcgtg gtgttaatga tcgcatcgat gattgcgggt gaaaaactga
    52561 cggcgctccc ttccctgtca ggcttccttg cggtcggcta tctggcgctg tttggttcga
    52621 ttatcgccat taacgcttat atgtatttaa tccgtaatgt cagtccggct ctcgccacca
    52681 gctacgccta cgttaacccg gtggtcgcgg tcttgctggg tacgggactg ggtggcgaaa
    52741 cgctgtcgaa gattgaatgg ctggcgctcg gcgtaattgt tttcgcggtg gtactggtca
    52801 cgttgggaaa atatctcttc ccggccaaac ccgttgtcgc gccagttatt caggatgcat
    52861 caagcgagta aatgaatccc ctgcgtgtcg atctgcgcgc tggcaccttc gccgcagatc
    52921 cactcttcca gacgctcggt aagcgccgca tccgtcagct tctcgcgcct acgtaacgca
    52981 cactcccaga caatcagtac gcgccagccg agctcctgca agcgactgat atcgcggcga
    53041 tcgcgttcaa catttttacc tatcttctcc agccagaact cagttcgagt cgcaggcact
    53101 ttaaacagat agcagtgatg atgatgccag aagcagccgt gggtaaatat cacgcagcga
    53161 tattcatcaa cgacaaaatc cgggcgtccg ggcagactgg cgtcctgaac gcgaaacgca
    53221 aggccctgcc cggttaacaa actggcgagg cgcttctcaa tcgccgtatc acgcgtggca
    53281 atcgcgcgca tatttttgct gcgggtggcc ttatcgtgaa cgtcgaccat gttgtgcctc
    53341 ttgctgacgc aacgccaccg cctgtttgat ttttggctca agcagttttg ccaccgcggc
    53401 aaagaccggc acgaccaccg agttgccgaa ctggcgatag gcctgagtgt ccgaaaccgg
    53461 aatgcggaat ttcgcttctc ccggcgcttc aaaacccatt aagcgcgcgc attcacgagg
    53521 cgttaaccga cgtggacgat gttgctgatt cagcggatcg tcaaagtctt tctcacccgt
    53581 ggccatatcc caaccgcgat cgattaaaat ttccgcgcca tctttgtaat aacgagcaga
    53641 cagcgtacgg gtgacgcttt gcggattgtt cggataaacc attccataac cgaagccgtt
    53701 accgcgcgcc tgatgttttt tcgcatatcg atagaggtac ttccacagca ccggcgtcag
    53761 gatatatttc gcctcaacca tcgggtccag cagctgcgcc agcgtcactc gctgtgcagg
    53821 gaaacattcg ctgatatcac gcagagtaaa atcggctttc agattcagat cgcgacgaaa
    53881 acccaccagc acgatgcgtt cacggtgctg cggcaggaaa tgtttaccat cgatgatttt
    53941 cggatcgtcc ggcccgttat cttctgcatc ggcaacgtca tagcccagtt cgtccagcgt
    54001 ctgcatgatg atgcggaacg tttttccctt atcgtgactc ttcaggtttt tgacgttttc
    54061 gagcacaaac atcgccggac gacgcgcgtc gataatgcgc accacatcaa agaacagcgt
    54121 gccctgggta tcgcaggcaa aaccgtgcgc ccgcccgagc gagttctttt tcgatacgcc
    54181 agccagcgaa aatggctgac aagggaaacc ggccagtaaa acatcgtgtt caggaatgtg
    54241 ttgacgaata tgttccgccg ccgcctcatc actcacgcct tcttgatgac tgagggtgat
    54301 gtcgcggata tcttcattaa aatgatgcgt cgccgggtcg caataatggt tggctttata
    54361 agtgcgtacc gcatgtttgt tccattcgct ggtaaacacg cactgtccac caatcgattc
    54421 aaaaccacga cggatgccgc caattccggc aaatagatcg ataaagcgaa acgcataatg
    54481 cggatgatgt tccggtggtt tgggtaacaa cgtttgcaga tgggcaaact ctttctcact
    54541 taaacggtgc catgccgagt cattcgccag cgcacgtttt aaaattgccg cgctccagtg
    54601 attctcaccc acaccattaa gctgcgccac cagcatttta acatcataaa tttgcagcag
    54661 tttctccagc attgcctgtg cggcattccc ggtgctgtat gaatcggtta ctgatatatt
    54721 ttcctgcata gatttaaccg gccatctgag atggccgaca ggttaacata attactgccg
    54781 tttagggcca cgcgctcagc cagcgggtga aaacgcatct atcaccttgt gatcgatacc
    54841 ttcattctcc cccgccagtt cggcaccgag cttcgccatc aaacagcgcc aagctcaatg
    54901 cccctgaaac ggcaaatacg cgcgccaggc caatcgcccc cagggcttcc agccgatccg
    54961 catcctggac gattttagct tccgttgtta agggggcgat ttgcgcgctg aaactgtgag
    55021 cggcaatggc atgacaaacg gcctcaattt tctccgccgg aaattgcacg aactcttcac
    55081 ggagcaggcg acgcgtctct tctgccgcca ggattgaaga acgctgccgt tgcggatgat
    55141 ttttcgccag gctcacgata tcgtgaaaat aacaggcggt taaaatcacc agcatatcga
    55201 catcgtcatc tgccgccagt ttctgtgccg tagcccagac acggcgaaaa tgacacaggt
    55261 catgcgccgc gtcctgatgt tgatgatggt ttttcaacca gttctcgaac tatgcctgcc
    55321 agtgttgtaa ctccatacgc gctccgttgc caaagagtct caaccttagc agtttttatt
    55381 catagcgtct gcaacgtgcg ggacgaggtc gggtgtacca tgcaatgccg cctaacaagg
    55441 caccaaccag ggccataaac agaaatccga ccaatgctcc taaccacttc cccgcggtag
    55501 atcctaaatc actttcaaat cccgacacgg gggcatcggg gatccggctc atcacccaga
    55561 tgtatcgcgc cattgcccag acgaaatagc cacaccatgt atagaaagcc c
//
LOCUS       DAETQL010000017        55420 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.017, whole genome shotgun sequence.
ACCESSION   DAETQL010000017 DAETQL010000000
VERSION     DAETQL010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.017"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="K0W65_001095"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="K0W65_001095"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBI3094447.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="K0W65_001096"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="K0W65_001096"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBI3094448.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="K0W65_001097"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="K0W65_001097"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBI3094449.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="K0W65_001098"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="K0W65_001098"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBI3094450.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="K0W65_001099"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="K0W65_001099"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBI3094451.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="K0W65_001100"
     CDS             7826..9559
                     /locus_tag="K0W65_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBI3094452.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="K0W65_001101"
     CDS             9708..10559
                     /locus_tag="K0W65_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3094453.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="K0W65_001102"
     CDS             complement(10546..10887)
                     /locus_tag="K0W65_001102"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBI3094454.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="K0W65_001103"
     CDS             complement(10889..11767)
                     /locus_tag="K0W65_001103"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBI3094455.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="K0W65_001104"
     CDS             complement(11733..14030)
                     /locus_tag="K0W65_001104"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBI3094456.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="K0W65_001105"
     CDS             complement(14081..14401)
                     /locus_tag="K0W65_001105"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBI3094457.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="K0W65_001106"
     CDS             complement(14416..15495)
                     /locus_tag="K0W65_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBI3094458.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="K0W65_001107"
     CDS             15804..16907
                     /locus_tag="K0W65_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBI3094459.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="K0W65_001108"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="K0W65_001108"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBI3094460.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="K0W65_001109"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="K0W65_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="K0W65_001110"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="K0W65_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="K0W65_001111"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="K0W65_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="K0W65_001112"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="K0W65_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBI3094461.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="K0W65_001113"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="K0W65_001113"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBI3094462.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="K0W65_001114"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="K0W65_001114"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBI3094463.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="K0W65_001115"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="K0W65_001115"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBI3094464.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="K0W65_001116"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="K0W65_001116"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBI3094465.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="K0W65_001117"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="K0W65_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBI3094466.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="K0W65_001118"
     CDS             30120..30728
                     /locus_tag="K0W65_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBI3094467.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="K0W65_001119"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="K0W65_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBI3094468.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="K0W65_001120"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="K0W65_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBI3094469.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="K0W65_001121"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="K0W65_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBI3094470.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="K0W65_001122"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="K0W65_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBI3094471.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="K0W65_001123"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="K0W65_001123"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBI3094472.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="K0W65_001124"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="K0W65_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBI3094473.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="K0W65_001125"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="K0W65_001125"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBI3094474.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="K0W65_001126"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="K0W65_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBI3094475.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="K0W65_001127"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="K0W65_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBI3094476.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="K0W65_001128"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="K0W65_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="K0W65_001129"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="K0W65_001129"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBI3094477.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="K0W65_001130"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="K0W65_001130"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBI3094478.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="K0W65_001131"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="K0W65_001131"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBI3094479.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="K0W65_001132"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="K0W65_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBI3094480.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="K0W65_001133"
     CDS             complement(44843..45142)
                     /locus_tag="K0W65_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBI3094481.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="K0W65_001134"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="K0W65_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="K0W65_001135"
     CDS             45895..46494
                     /locus_tag="K0W65_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBI3094482.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKIQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="K0W65_001136"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="K0W65_001136"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBI3094483.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="K0W65_001137"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="K0W65_001137"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBI3094484.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="K0W65_001138"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="K0W65_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HBI3094485.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="K0W65_001139"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="K0W65_001139"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBI3094486.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="K0W65_001140"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="K0W65_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBI3094487.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="K0W65_001141"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="K0W65_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBI3094488.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="K0W65_001142"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="K0W65_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBI3094489.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="K0W65_001143"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="K0W65_001143"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBI3094490.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="K0W65_001144"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="K0W65_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBI3094491.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13494 a        15017 c        14165 g        12744 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcgggatca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggc gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatgtggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg gcccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccagacgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttatttacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaatacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttac tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtgggga
//
LOCUS       DAETQL010000018        55369 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.018, whole genome shotgun sequence.
ACCESSION   DAETQL010000018 DAETQL010000000
VERSION     DAETQL010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.018"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..356
                     /locus_tag="K0W65_001145"
     CDS             <1..356
                     /locus_tag="K0W65_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBI3094492.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="K0W65_001146"
     CDS             complement(394..1293)
                     /gene="nhaR"
                     /locus_tag="K0W65_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBI3094493.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="K0W65_001147"
     CDS             complement(1359..2525)
                     /gene="nhaA"
                     /locus_tag="K0W65_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBI3094494.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3111..3263
                     /gene="mokC"
                     /locus_tag="K0W65_001148"
     CDS             3111..3263
                     /gene="mokC"
                     /locus_tag="K0W65_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBI3094495.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="K0W65_001149"
     CDS             complement(3367..4497)
                     /gene="dnaJ"
                     /locus_tag="K0W65_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBI3094496.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="K0W65_001150"
     CDS             complement(4586..6502)
                     /gene="dnaK"
                     /locus_tag="K0W65_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBI3094497.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6879..7283
                     /locus_tag="K0W65_001151"
     CDS             6879..7283
                     /locus_tag="K0W65_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBI3094498.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7309..8022
                     /gene="msyB"
                     /locus_tag="K0W65_001152"
     CDS             7309..8022
                     /gene="msyB"
                     /locus_tag="K0W65_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBI3094499.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8171..8737
                     /gene="satP"
                     /locus_tag="K0W65_001153"
     CDS             8171..8737
                     /gene="satP"
                     /locus_tag="K0W65_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBI3094500.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8772..9359)
                     /gene="mog"
                     /locus_tag="K0W65_001154"
     CDS             complement(8772..9359)
                     /gene="mog"
                     /locus_tag="K0W65_001154"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBI3094501.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9474..10427)
                     /gene="tal"
                     /locus_tag="K0W65_001155"
     CDS             complement(9474..10427)
                     /gene="tal"
                     /locus_tag="K0W65_001155"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBI3094502.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10706..12136
                     /locus_tag="K0W65_001156"
                     /pseudo
     CDS             10706..12136
                     /locus_tag="K0W65_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12206..12982
                     /gene="yaaA"
                     /locus_tag="K0W65_001157"
     CDS             12206..12982
                     /gene="yaaA"
                     /locus_tag="K0W65_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBI3094503.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13135..13431)
                     /locus_tag="K0W65_001158"
     CDS             complement(13135..13431)
                     /locus_tag="K0W65_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBI3094504.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13645..14931)
                     /gene="thrC"
                     /locus_tag="K0W65_001159"
     CDS             complement(13645..14931)
                     /gene="thrC"
                     /locus_tag="K0W65_001159"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBI3094505.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14932..15864)
                     /gene="thrB"
                     /locus_tag="K0W65_001160"
     CDS             complement(14932..15864)
                     /gene="thrB"
                     /locus_tag="K0W65_001160"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBI3094506.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15866..18328)
                     /gene="thrA"
                     /locus_tag="K0W65_001161"
     CDS             complement(15866..18328)
                     /gene="thrA"
                     /locus_tag="K0W65_001161"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBI3094507.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKNISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSLSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18354..18473)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18409..18474)
                     /gene="thrL"
                     /locus_tag="K0W65_001162"
     CDS             complement(18409..18474)
                     /gene="thrL"
                     /locus_tag="K0W65_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBI3094508.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18688..19374)
                     /locus_tag="K0W65_001163"
     CDS             complement(18688..19374)
                     /locus_tag="K0W65_001163"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBI3094509.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19774..19914)
                     /locus_tag="K0W65_001164"
     CDS             complement(19774..19914)
                     /locus_tag="K0W65_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094510.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20010..20726
                     /gene="arcA"
                     /locus_tag="K0W65_001165"
     CDS             20010..20726
                     /gene="arcA"
                     /locus_tag="K0W65_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBI3094511.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20785..22137)
                     /gene="creD"
                     /locus_tag="K0W65_001166"
     CDS             complement(20785..22137)
                     /gene="creD"
                     /locus_tag="K0W65_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBI3094512.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22195..23619)
                     /gene="creC"
                     /locus_tag="K0W65_001167"
     CDS             complement(22195..23619)
                     /gene="creC"
                     /locus_tag="K0W65_001167"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBI3094513.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23619..24308)
                     /gene="creB"
                     /locus_tag="K0W65_001168"
     CDS             complement(23619..24308)
                     /gene="creB"
                     /locus_tag="K0W65_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBI3094514.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24321..24794)
                     /gene="creA"
                     /locus_tag="K0W65_001169"
     CDS             complement(24321..24794)
                     /gene="creA"
                     /locus_tag="K0W65_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBI3094515.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25005..25874
                     /gene="robA"
                     /locus_tag="K0W65_001170"
     CDS             25005..25874
                     /gene="robA"
                     /locus_tag="K0W65_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBI3094516.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25871..26518)
                     /gene="gpmB"
                     /locus_tag="K0W65_001171"
     CDS             complement(25871..26518)
                     /gene="gpmB"
                     /locus_tag="K0W65_001171"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBI3094517.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26570..27090
                     /gene="yjjX"
                     /locus_tag="K0W65_001172"
                     /pseudo
     CDS             26570..27090
                     /gene="yjjX"
                     /locus_tag="K0W65_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27175..27501)
                     /gene="trpR"
                     /locus_tag="K0W65_001173"
     CDS             complement(27175..27501)
                     /gene="trpR"
                     /locus_tag="K0W65_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBI3094518.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27591..29528)
                     /gene="sltY"
                     /locus_tag="K0W65_001174"
     CDS             complement(27591..29528)
                     /gene="sltY"
                     /locus_tag="K0W65_001174"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBI3094519.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29739..31406
                     /gene="ettA"
                     /locus_tag="K0W65_001175"
     CDS             29739..31406
                     /gene="ettA"
                     /locus_tag="K0W65_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBI3094520.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFTPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31513..31680)
                     /locus_tag="K0W65_001176"
     CDS             complement(31513..31680)
                     /locus_tag="K0W65_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094521.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31714..32946)
                     /gene="nadR"
                     /locus_tag="K0W65_001177"
     CDS             complement(31714..32946)
                     /gene="nadR"
                     /locus_tag="K0W65_001177"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HBI3094522.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32967..34349)
                     /gene="radA"
                     /locus_tag="K0W65_001178"
     CDS             complement(32967..34349)
                     /gene="radA"
                     /locus_tag="K0W65_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBI3094523.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34398..35366)
                     /gene="serB"
                     /locus_tag="K0W65_001179"
     CDS             complement(34398..35366)
                     /gene="serB"
                     /locus_tag="K0W65_001179"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBI3094524.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35472..36116
                     /locus_tag="K0W65_001180"
     CDS             35472..36116
                     /locus_tag="K0W65_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBI3094525.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36144..37159
                     /gene="lplA"
                     /locus_tag="K0W65_001181"
                     /pseudo
     CDS             36144..37159
                     /gene="lplA"
                     /locus_tag="K0W65_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37191..37454
                     /locus_tag="K0W65_001182"
     CDS             37191..37454
                     /locus_tag="K0W65_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3094526.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37615..38334)
                     /gene="deoD"
                     /locus_tag="K0W65_001183"
     CDS             complement(37615..38334)
                     /gene="deoD"
                     /locus_tag="K0W65_001183"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBI3094527.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38391..39614)
                     /gene="deoB"
                     /locus_tag="K0W65_001184"
     CDS             complement(38391..39614)
                     /gene="deoB"
                     /locus_tag="K0W65_001184"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBI3094528.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39666..40988)
                     /gene="deoA"
                     /locus_tag="K0W65_001185"
     CDS             complement(39666..40988)
                     /gene="deoA"
                     /locus_tag="K0W65_001185"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBI3094529.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41115..41894)
                     /gene="deoC"
                     /locus_tag="K0W65_001186"
     CDS             complement(41115..41894)
                     /gene="deoC"
                     /locus_tag="K0W65_001186"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBI3094530.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42152..43693
                     /locus_tag="K0W65_001187"
     CDS             42152..43693
                     /locus_tag="K0W65_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBI3094531.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43665..44528
                     /locus_tag="K0W65_001188"
     CDS             43665..44528
                     /locus_tag="K0W65_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBI3094532.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44742..45524)
                     /locus_tag="K0W65_001189"
     CDS             complement(44742..45524)
                     /locus_tag="K0W65_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBI3094533.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45521..46594)
                     /locus_tag="K0W65_001190"
     CDS             complement(45521..46594)
                     /locus_tag="K0W65_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBI3094534.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46716..46877)
                     /locus_tag="K0W65_001191"
     CDS             complement(46716..46877)
                     /locus_tag="K0W65_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBI3094535.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46994..47599)
                     /gene="osmY"
                     /locus_tag="K0W65_001192"
     CDS             complement(46994..47599)
                     /gene="osmY"
                     /locus_tag="K0W65_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBI3094536.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47992..49581)
                     /gene="prfC"
                     /locus_tag="K0W65_001193"
     CDS             complement(47992..49581)
                     /gene="prfC"
                     /locus_tag="K0W65_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBI3094537.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49672..50349)
                     /gene="yjjG"
                     /locus_tag="K0W65_001194"
     CDS             complement(49672..50349)
                     /gene="yjjG"
                     /locus_tag="K0W65_001194"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBI3094538.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50364..50810)
                     /gene="rimI"
                     /locus_tag="K0W65_001195"
     CDS             complement(50364..50810)
                     /gene="rimI"
                     /locus_tag="K0W65_001195"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBI3094539.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50779..51192)
                     /gene="holD"
                     /locus_tag="K0W65_001196"
     CDS             complement(50779..51192)
                     /gene="holD"
                     /locus_tag="K0W65_001196"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBI3094540.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51295..52326
                     /gene="rsmC"
                     /locus_tag="K0W65_001197"
     CDS             51295..52326
                     /gene="rsmC"
                     /locus_tag="K0W65_001197"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBI3094541.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            52603..52689
                     /locus_tag="K0W65_001198"
     tRNA            52603..52689
                     /locus_tag="K0W65_001198"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52637..52639,aa:Leu,seq:cag)
     gene            52718..52804
                     /locus_tag="K0W65_001199"
     tRNA            52718..52804
                     /locus_tag="K0W65_001199"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52752..52754,aa:Leu,seq:cag)
     gene            52839..52925
                     /locus_tag="K0W65_001200"
     tRNA            52839..52925
                     /locus_tag="K0W65_001200"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52873..52875,aa:Leu,seq:cag)
     gene            complement(52964..53200)
                     /locus_tag="K0W65_001201"
     CDS             complement(52964..53200)
                     /locus_tag="K0W65_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBI3094542.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            53341..54129
                     /gene="fhuF"
                     /locus_tag="K0W65_001202"
     CDS             53341..54129
                     /gene="fhuF"
                     /locus_tag="K0W65_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBI3094543.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(54167..54844)
                     /gene="bglJ"
                     /locus_tag="K0W65_001203"
     CDS             complement(54167..54844)
                     /gene="bglJ"
                     /locus_tag="K0W65_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBI3094544.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(54802..>55369)
                     /locus_tag="K0W65_001204"
     CDS             complement(54802..>55369)
                     /locus_tag="K0W65_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI3094545.1"
                     /translation="LVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQA
                     VELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILT
                     LSERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQG
                     ARELNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        13249 a        14944 c        13815 g        13361 t
ORIGIN      
        1 attatcagtt tcatcatata agatttgact gcatccaggg cgagcaaggt gaattagttt
       61 ttgaacccag tgacgttgaa tattactttg aaccagtgac gatacaggag tcaggaacca
      121 gcatattaaa aaatgagtta gaaaacagtg aggacggtgc gggtcaaata ggctttcaac
      181 tctccaatga tggtacgaat gaaatccagt atggtaaaag caattactac tcttttcagc
      241 atccccatga aggaagtaat cagattcctc tatttattcg cccgcgtaca tatggaaaca
      301 atgtctcaag cgggcaaata atgagccgag tcaaaatcgt ggtaatgtac aactaatcta
      361 tctcccccac cttaactttg ggggagctgc cgattaacgc accgctggac taaaaagcgc
      421 agaataatcc gtattgcaga ttcgctgtac cgccgggtgc tgaatcatcc gctcagcaaa
      481 aatagcatgg tactcttcca tcacattctc gacgcgacca atttctacga cagttttatc
      541 ggcataaaag tcatatgcat aaagcgttgg ggcaacgaag attgcattgt gcatcgcacc
      601 aaaagctttc atcaaagcgg catcatcaaa ctcgccgagg atttctacgt ttaatccctg
      661 ggagttaaac cagttaagca atttgcgccc taacattgaa cgtcgcccag gaatcaaaag
      721 tcgccgttct tccagacaag ccgggaacgg tttttctggt ggtggatttg tacaccagaa
      781 actcacgcca cattcgccaa ttctcacgga gaacaggcct tcctgctgcg tagagtctat
      841 cggacagtca gaaatgatca tatccagttt atgctgactt aattgctcca gcagcatttc
      901 gtgggtggat tcgaagcagc gaagatgaat gggctcgcct tctaccactg cggcgttaag
      961 tacgctactg accaggcgtt tggaaagtgc atcagccacg ccaacgtcaa acaataaatt
     1021 ggattctttg cgatagttca caatatccag catttcctgg cttaaggtga acattttatc
     1081 ggcatagcga tagaccagtt ctcccagctc gctgggttcg agaccacgtc ccttgcgttt
     1141 aaataatttg ccttgcaggc gctcttccag cgctcgaatc tgtccggtaa tggtttgtgg
     1201 agttaaataa agcgcctccg ctgcgccaac cacggaacct tctttataga catgccagaa
     1261 gtaatacaag tggttgtaat tgatatgaga catgctcatt tctctccctg ataacaatga
     1321 aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc aaactgatgg acgcaaacga
     1381 acgcgtaacc agctgtatcc aattaccgcc gaagagatag aaccgaccag gataccgagt
     1441 ttcgcccagt taatcagttc tggatctacg ctaccaaagg ccaggctggc aataaagata
     1501 gacatagtaa aaccgatacc gcacaggatc cccaccacca taatttgctg ataagtcgtt
     1561 ccctcaggca gatgcgccag tttcaaacgc agcgccaacc agcagaacag actaatcccc
     1621 agcggtttgc caatcagcaa gccagcgatg atccccaatg gcagaatgga ggtcaagcca
     1681 tccagcgtga cgccttgcag tgaaacgcca gcattagcaa atgcaaacag cggcaaaatc
     1741 agatacgcca cccacgggtg caacacatgc tccagtcgct tcgctggaga acgcccatgc
     1801 ttctctttca aaggaataaa gaagccgaca attacccccg ccagagttgc gtgaaccccc
     1861 gatttcaaca ccgcagtcca caacaccacg ccaacaagaa tatagacgcc cgtgcggcgt
     1921 acaccacaca gattcaatac cgcgagtacc gcaattgcta cagccgcgac gccaagggag
     1981 gccatcgata agtcattagt gtagaacaat gcgatgataa tgatggcccc aagatcgtcg
     2041 ataatagcca gagccatcaa aaagatcttc agcactaacg gaacacgact tcccaacagc
     2101 gccagtacac caagtgcaaa agcaatgtca gtagccgccg ggatcgccca cccttcgcgg
     2161 gtaatcggat cggcatagtt aaaagccaga tagagtaatg ccggcacaat catcccacca
     2221 atagcggcga taactggaaa tgcggcctgg cgtaagctgg ctagcgatcc ttgcatcagt
     2281 tcacgtttaa cttccagacc gactaacagg aaaaataccg ccatcagcgc atcatttatc
     2341 cataacagca tgtttttgtt gatttcgagt gaaccaaccc ggagctgaac cggcgtctcc
     2401 agaaagtcgt gataccatcc actggttgcg ccgctgttgg ccataatcat cgccaggata
     2461 gcggcaatga taagaataat gcctcccgag gcatcactgc taaagaatcg atgcagatgt
     2521 ttcacttttt atttctcttt caggtgaata gatcgttttt actattttac cccgcacgaa
     2581 tcatcgttaa aatcagatta tagtgtagga ataattcgtt ttttacgagc cattgaagaa
     2641 tttgcgccat cagcggtgag atgcgttttt gacggctgta agcgctcacg agtgacccta
     2701 agcccagata atcatagctt aaggtgagga ttcctgaggg attaattagg aaaagtaata
     2761 attaatttgt tgtcagtcgg cgatgcttgt taggccaata tttcaaaaat aatccagctt
     2821 gtcgtcaata attgcgatga agagataaat taaagttaaa taattaaatc attaaaaaat
     2881 tacgccacaa aaatgcgagg cgtcttcagg attagaatcg ataaaacaaa atgggttaaa
     2941 gagagcgata taatagcggc gggtgcttga ggctgtctgt ctcaggcatt agctgaacgg
     3001 cagatagaga aaagccccga gtgatatttt accatcaacc cgatgcctcc tatatgctga
     3061 acacatgtag agtgcctctt actgaccgta aggtcaagga gaagagagca atgaagcagc
     3121 ataaggcgat gattgtcgcc ctgatcgtca tctgtatcac cgccgtagtg gcggcgctgg
     3181 taacgagaaa agacctctgt gaggttcaca tccgaactgg ccagacggag gttgctgttt
     3241 tcacggctta cgaatccgag taagagcaac ggcggggagt gatccccgcc actttacagg
     3301 tgctcgcata tcttcaacgc accctatttt tacccaggcc tgcccacggg caggcttttg
     3361 gggaggttag cgggtcaggt cgtcaaaaaa cttcttcaca ccatcaaaga agctctttga
     3421 gcgcgggctg ttgtgctcgc cggttgggcc accgaagctt tcttgcagct cttgcagcag
     3481 ctgtttctgc ttttcgttca ggcctaccgg tgtttcgacg acaacgcggc acagcaaatc
     3541 accctgtgcg ccaccgcgga cagacttgac gcctttaccg cgcatacgga atagcttacc
     3601 ggtctgggtt tcgccaggca ctttcagttt gacgcgacca tcaagggtcg gtacttcgat
     3661 ttcgccaccc agcgccgcca tagcgaagtt gatcgggact tcgcaataca ggttgttgcc
     3721 ttcacgctcg aaaatcgggt gctgtttaac ctgaacctga acgtacagat cgcctgccgg
     3781 tgcgccatgc tcgcccgctt caccttcgcc cgcaagacgg atgcggtctc cagtgtccac
     3841 ccctgccggg attttaacgg acagcgtttt gctgcgctca acacgaccat gaccatgaca
     3901 tttgttgcac ggatctttga tcagcgtacc gcggccctga cagtgtggac aggtctgctg
     3961 tacagcgaag aatccctggc gcatctgcac ctgaccagaa ccatgacagg tcggacaggt
     4021 ctgcggctgt gtacctggtt ttgcaccgct accgtggcaa acgtcacact cttccagagt
     4081 cggaatgcgg atctctttgg tcacgccacg tacagcttct tcgagggtga gctccatgtt
     4141 atagcgtaaa tcagcaccgc gcgccgcacg ttgacgacca cgtccgccgc caaaaatatc
     4201 gccgaaaacg tcaccaaaaa tatcgctgaa gtctgcgccg ccgccaaaac caccgccgcc
     4261 catgcctcct tgctcaaacg cagcatgacc atactgatcg tatgccgcac gtttttgcga
     4321 gtcggtcaga acttcataag cttccttgat ctctttaaat ttcgcctcgg cctctttgtc
     4381 accctggtta cggtccgggt ggtatttcat ggccaggcgt ttgtaggcct ttctgatttc
     4441 acgctcttcc gctgttttgg aaacgcctaa aatctcgtaa taatcttgct tagccatctt
     4501 ttttaaattg cccctagatg aatgcacggg cggagaggaa attccccttc gcccgtgtca
     4561 gtataattac ccgtttatag ggcgattatt ttttgtcttt gacttcttca aattcagcgt
     4621 cgacaacatc gtcatctttc gcgttgtttg cagaagcatc agcaccggca gtctgctgct
     4681 gggcatgttg ctgctgggcg atttccatca gtttctggga aacctgtgcc agttcctgca
     4741 ttttcgcttc gatagcggct ttgtcttcac ctttcagagc agtttccagt gcagtcagcg
     4801 cagactcgat agcagttttg tcgtcagccg gcagtttgtc gcctgcttct tcaacctgct
     4861 tacgggtgct gtgcagcaga tggtcgccct ggttgcgagt ctgtaccagc tcttcaaact
     4921 tacggtcagc ttcggcgtta gcttctgcgt cgcgtaccat tttctggatt tcatcttcgt
     4981 tcagaccaga agaagccttg atggtgatct tctgctcttt accgctgttt ttatctttcg
     5041 cggaaacgtg caggataccg tcagcatcga tatcgaaggt aacttcgatc tgcggcatgc
     5101 cgcgcggtgc cgggttgata ccatctaggt tgaactgacc cagagatttg ttatcagccg
     5161 cacgtttacg ttcaccctgc agcacatgga tggttaccgc agactggttg tcttcagcgg
     5221 tagagaacac ctggctgtgc ttggtcggga tagtggtgtt tttcgcgatc agcgtcgtca
     5281 tcacaccgcc catggtttcg atacccagag acagcggggt aacgtccagc agcagtacgt
     5341 cttttacgtc accagtcaga acaccaccct gaacagcagc accgattgct acagcttcgt
     5401 ccgggttaac gtctttacgc ggctctttac caaagaactc agcaactttc ttctgaacca
     5461 ttggcatacg agtctgacca ccaacgagga taacgtcgtc gatatcagat acggacaggc
     5521 cagcatcctg cagtgcaact ttcagcggct caatggaacg gtttaccaga tcttcaacca
     5581 ggctttccag tttcgcacga gtcactttga tgttcatgtg tttcggaccg gtcgcgtctg
     5641 cagtgatgta tggcaggtta acgtcggtct gctgagcgga agacagttcg attttcgctt
     5701 tttctgccgc ttctttcagg cgctgcattg ccagcggatc gttgcgcagg tcaatgccct
     5761 gatctttctt gaattcttca accagatagt tgatcagacg gctgtcgaag tcttcaccac
     5821 ccaggtgggt atcaccgttg gttgccagaa cttcgaaggt tttttcgccg tcaacttcgt
     5881 cgatttcgat aatagaaata tcgaaagtac caccccccag gtcataaacc gcgatagtac
     5941 ggttgccagt gcctttgtcc agaccgtaag ccagcgcagc tgcggtcggt tcgttgatga
     6001 tacgttttac ttccagacca gcgatacggc ctgcgtcttt ggttgcctga cgctgagcat
     6061 cgttaaagta tgccggtacg gtgataacag cttcagttac cggttcaccc aggtaatctt
     6121 cagcggtttt cttcattttt ttcagcactt cagcagaaat ctgcggcggt gccattttct
     6181 ggcctttaac ttcgacccat gcgtcgccgt tatcagcagc aataattttg aacggcatga
     6241 tggaaacatc acgctgtact tcttcgtcct ggaagcggcg gccaatcagg cgtttaatcg
     6301 caaacagggt gttttgcggg ttcgtcactg cctgacgttt agccggctga ccaaccagag
     6361 tttcaccatc ctgggtatag gcaatgatag aaggcgtggt gcgatcgcct tcggcgttct
     6421 ccagcacgcg aggaatggtg ccatccataa tcgctacaca agagttggta gtacccaggt
     6481 cgataccaat tattttaccc atctaaacgt ctccactata tattcggtca tcatgtggtt
     6541 gtgagtctgt aataggggcg aaacgtctgg tttcaactgc ccaatttcat ttttttgcag
     6601 actcactcac tgcggttgac tactaaatgg ggtcgtaacc cacgtcatca agggggagat
     6661 gcaaaaattt tttgtgcttt tgcgcagaaa tttcgcgata tggcaggaca agacgtaaaa
     6721 ttgacagcaa ttttattcat ataaagaatg aatcgcagcc aaggcgttca tcaaaaaatt
     6781 gtaataaaaa gaaaagatta cgtgcctgaa tcttctcttt atcagcagta aactagtggg
     6841 tattcatccc cctacctctt cccactaaga gaatccttat gaaatccgtt tttacgattt
     6901 ccgccagcct ggcgattagc ctgatgctgt gctgcacggc gcaggcaaac gaccataaac
     6961 tcctcggcgt cattgcaatg ccgcgtaacg aaaccaacga tctggcgctg aaacttcctg
     7021 tttgtcgcat tgtgaaacgc atacaactct ctgccgacca tggcgattta cagttaagtg
     7081 gtgcatcggt ttatttcaaa gccgcccgta gcgccagtca gagcctgaat attccttcag
     7141 aaataaaaga agggcaaacc actgactgga tcaacattaa cagcgataac gacaataaac
     7201 gctgcgtctc aaaaatcacc ttttccggtc atacggtgaa ctcatcggat atggccacgc
     7261 tgaaaattat cggcgacgac taacgccaga atatagaagc cacaaaaaat gaatgttaat
     7321 tacctgaatg attcagatct ggattttctt cagcattgta gtgaggaaca gttggcaaat
     7381 ttcgcccgat tgctcaccca taatgaaaaa ggcaaaactc gcctctccag catactgatg
     7441 cgcaacgaac tgtttaaatc gatggaaggg catcccgagc aacatcgccg caactggcag
     7501 ctgattgccg gagaattaca gcattttggt ggcgatagta tcgccaacaa actgcgcgga
     7561 cacggtaaat tgtatcgggc cattttgctc gatgtttcaa agcgattgaa gctgaaagcc
     7621 gacaaagaga tgtctacgtt tgaaattgag caacagttac tggaacaatt tctgcgtaat
     7681 acctggaaga aaatggacga ggaacataag caggagtttc tgcacgcggt cgatgccagg
     7741 gtgaatgagc tggaagagct gctgccgctg ctgatgaaag acaaattatt ggcaaaaggt
     7801 gtgtcgcatt tgctttccag ccaactgacc cgcattttac gcacccacgc agcaatgagc
     7861 gtacttgggc atggtttgct gcgcggcgcg gggctgggag gccctgtggg cgcagcatta
     7921 aatggggtta aagcggtcag cggcagcacc tatcgcgtga cgattccagc cgtactgcaa
     7981 atcgcctgcc tgcgccggat ggttagcgcc actcaggtct gataccggaa accggggaaa
     8041 attttcattt tcccctcaaa agatcgtaga cactgcccca ctggctgatt attatgccgc
     8101 gccctgaaaa cactacagtt attcagggaa attatttcac cattcattcg atgatgattt
     8161 ttgaggaatt atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt
     8221 cggcatgacc accattctgc ttaacctgca caacgtgggt tatttcgctc tggacggtat
     8281 tattcttgcc atgggcattt tctacggcgg catcgcgcaa atttttgctg gtctgctgga
     8341 gtacaaaaaa ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggct
     8401 gacgctggtt gcgattctgc tgatgccgaa actgggtctg accgatgcgc caaatgcaca
     8461 gttccttggt gtctacctgg gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac
     8521 tctgaaaggc gcgcgcgtcc tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct
     8581 gctggcgatc ggtaacattg ccggtaatgc cgcgatcatc cactttgccg gctggattgg
     8641 tctgatctgc ggtgccagcg caatctatct ggcgatgggt gaagtgctga acgagcagtt
     8701 tggtcgcacc gttctgccga ttggtgaatc ccactaattg tcttgagatc cccccgctcg
     8761 gggggatttt tttattcgct aacgtcgcgt cttgcactct tcggtctgaa tgcagcaacc
     8821 acttctggtg ccgtttcaac gtatggccct tccagcaact gaatgcagta cggtacgctg
     8881 gcaaaaatac catgcaccac aacgttaccc tcagcgtcct tcacaccttc cagcgtctct
     8941 ttaatagact tcggctgacc gggtaagtta aggatcagcg cctgtttgcg aatcacgccc
     9001 acctgacgcg aaaggatcgc agttggtaca aaatgcaggc tgatctggcg catctgttca
     9061 ccaaagccag gcatctcgcg gtccgctact gccagcgtcg catcgggcgt tacgtcacga
     9121 cgcgccgggc cagttccgcc cgtggtgagc accagatggc aactcatttc atccaccagc
     9181 tcacacaacg tttgctcgat gatcgcctgc tcatcgggga ttaagcgggt ttccagttca
     9241 aacggcgtgg ttagcgccga tgtcagccat tcttccagcg ctggaatgcc tttatcctga
     9301 taaacgccgc tggatgcgcg atcagagatg gaaactaagc caatacgtaa agtattcatc
     9361 aagatttttc cgtttaaaca atctcgttaa acagaatgat acactgcgaa gggagtgaca
     9421 gacaggcttc agaagaggta gcgtgaccga cttcccggtc acgctaagaa tgattacagc
     9481 agatcgccga tcattttttc cagtttttcc tggtcaatag caaacttacg gataccttcc
     9541 gccagtttat ctactgccat tggatcctgg ttgtgctgcc acaggaactc ggactcagtg
     9601 atacgcgccg gacgcgcttt cacttcgccg gtgtaagaca gtttacgttc gatagcccct
     9661 tcgctctccg ccagctcttt cagcagtgcc ggtgcgatgg tcagacggtc acagcctgcc
     9721 agttccagaa tttcgccgat gttacggaag cttgcgccca taaccacggt ttcataaccg
     9781 tgctctttgt agtactggta gatttcagat acagaaacca cgcccggatc ttctgccgga
     9841 gcgtactctt tcttatcggt attcgctttg taccagtcaa gaatacggcc aacaaacggc
     9901 gagatcagga acacgcccgc ctccgcacaa gcacgagcct gagcgaagga gaacagcagg
     9961 gtcaggttac agttgatacc ttctttttcc agctgttctg cagcacggat accctgccag
    10021 gtagaagcca gtttgatcag aatacgatcg ttgctaatac ctgcatcgtt gtagagtttg
    10081 atcaggcgtt ttgctttcgc aattgacgct tcagtgtcat aggaaagacg cgcatcaact
    10141 tcagttgaga tacggcccgg aaccagtttc aggatttcca gaccaatatt tactgccagt
    10201 ttgtcggtcg cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg
    10261 acagcatcat caatcaactt acggtattcc ggaatctgcg ctgcgttaag aatgagagaa
    10321 gggttggttg tggcatcctg cggttgatac agcttcattg ccgcgatgtc cccagtgtcg
    10381 gccactacgg tggtgtactg acgaagggag gtcaatttgt ccgtcatgat agtatttctc
    10441 tttaaacagc ttgttagggg gatgtaaccg gtctgccctg atgatatcac gacgcaacgt
    10501 cagcgcaacc gccgacaaga cggttatcgc caggcgagac tgtttcggat ttctgacact
    10561 gcgttgatat cgcccaccat ttttatacaa aacctcatgt atgctacgca gaagttatca
    10621 agtacctcgt agcgtatata cttcttaaac aattggtaac gtttacacag gaaagtcatc
    10681 gcgaccggca ataagaggga tatgcatgcc agattttttc tcctttatta acagcgtcct
    10741 ttggggatcg gtaatgattt acctgctctt cggcgcaggt tgttggttca cttttcgcac
    10801 cggatttgtg cagtttcgct acatccgcca gtttggcaaa agtcttaaaa atagcattca
    10861 tccacagcca ggcggtttaa cctcatttca gtcattgtgt accagtcttg cggcgcgcgt
    10921 gggtagcggc aacctggccg gcgttgcgct ggcaattacc gccggtggac caggtgccgt
    10981 cttttggatg tgggttgctg cgtttatcgg catggcgacc tcgtttgccg aatgttccct
    11041 tgcacaactt tataaagaac gtgacgtcaa tgggcagttt cgtggcggac cggcatggta
    11101 tatggcgcgc gggctgggga tgcgctggat gggcgttctg ttcgccgtct ttttgctcat
    11161 cgcctacggc ataattttca gcggagttca ggcgaacgcc gttgcccgag ccctgagttt
    11221 ttcttttgat tttcctccgc tggtgacggg cattattctc gctgtctttg ctctgctggc
    11281 aatcactcgt ggtcttcatg gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc
    11341 gataatctgg gtactgacca gcctggtgat ttgcgtaata aatatcgggc aacttcccca
    11401 cgtcatttgg tctatttttg agagtgcttt tggctggcag gaagcggcag gcggcgcggc
    11461 gggatatacc ttaagccagg cgattactaa cggttttcag cgcagtatgt tttccaatga
    11521 ggtgggaatg ggatcgacgc caaacgcggc agcggcagct gcatcctggc ctccgcatcc
    11581 ggcagcacaa gggattgtcc agatgattgg catttttatc gacaccctgg tcatctgtac
    11641 ggcaagcgcc atgctgatat tactggcggg taacggcaca acctacatgc cgctggaagg
    11701 tattcagctt atccagaagg cgatgcgggt gctaatgggt tcctagggtg ctgaatttgt
    11761 taccctcgtg gttattctgt ttgccttcag ctccatcgtt gccaactaca tttatgccga
    11821 aaacaatctc ttctttttac gcctgaacaa ccctaaagcg atctggtgtt tgcggatctg
    11881 caccttcgca acggtcatcg gcggcacctt gctaagcctt ccgctgatgt ggcaactggc
    11941 agatatcata atggcctgca tggctattac caatttgacc gccattttac tgctctcgcc
    12001 tgtggttcat accattgcca gtgattatct acgccagcgt aaactcggcg tgcgcccggt
    12061 gtttgatccg ttgcgttatc cggatatcgg tcgccagctt tctccggacg cgtgggatga
    12121 tgtttcgcag gagtaatcac aactatcgat caactcattc tctttttttg ctaaagtcgg
    12181 cataaatttc ctgcaaggac tggatatgct gattcttatt tcacctgcga aaacgcttga
    12241 ttaccaaagc ccgttgacca ccacgcgcta tacgctgccg gagctgttag acaattccca
    12301 gcagttgatc catgaagcgc ggaaactgac gcctccgcag attagcacgc tgatgcgcat
    12361 cagcgacaaa ctggcgggta tcaacgccgc tcgctttcat gactggcagc cagatttcac
    12421 gccggcgaat gcccgccagg cgattctggc gtttaaaggt gatgtctaca ccggcttgca
    12481 ggccgaaacc ttcagcgaag acgatttcga ttttgcccaa cagcatttgc gaatgctttc
    12541 cggcttgtat ggcgtactgc gcccgctcga tttaatgcag ccttatcgtc tggaaatggg
    12601 gatccgtctt gagaatgccc gagggaaaga tctgtatcaa ttctggggag atatcatcac
    12661 caacaagctg aacgaggcgc tcgcagcaca aggcgataat gtggtgatta atctggcgtc
    12721 agatgagtat tttaaatcgg tgaagccgaa gaaattgaat gccgagatta tcaagccggt
    12781 gttcctcgat gagaagaacg gcaaatttaa gatcatcagc ttctacgcta agaaagcacg
    12841 cggtctgatg agtcgtttca ttattgaaaa tcggctgacc aaaccagagc aactgactgg
    12901 ttttaatagc gaaggttact tctttgatga agattcctcc agcaatggcg aactggtgtt
    12961 taaacgctac gagcagcgtt aagtttgtag tcaatgccgg atgcggcgtg aacgccttat
    13021 ccggcctaca aaagcatgca gattcaatat attgcagaat taacgtaggc ctgataagac
    13081 gcgccagcgt cgcatcaggc gctgaatgcc ggattgttac ccggcatttg tcatttagcg
    13141 gtgatgtttg cctggaccat gaccgccgtg atgatcatga ggagctttct tatggtggcg
    13201 cggcggtggc ggcggtccgt gtgggtgcca gcgattgcct cgccattcat aatgttgttt
    13261 ccaccagccg tggtcgcgcc agtgacctcc atcccagtaa tagccacgat tatcacgatc
    13321 gcctatctgt aattttactg acgggactaa cgtaatttcc gcagcctgtg ctgccatggg
    13381 agcgaccaga accagggaaa gtgcgagtac gatagattgc atctttttca caggttactc
    13441 cttttatcat tgcccgttat gggtcgatat ccggagatta cgcgatgaga acgacggttg
    13501 ttattctccg caatcctaat ctctaagtta ccgcatttat tgataatttc tcctttttcc
    13561 ctgtcgtttt tctccataat ttcttcataa aaaagccggg ctgcataaaa gcaaacccgg
    13621 cctgattgag ataatgaata gattttactg atgattcatc atcaatttac gcaacgcaga
    13681 aaaatcggcg ggcagattat gtgaaagcaa gggtaaatca gcacgttctg ccagctcttt
    13741 tggcagatcc aacgtttcac cgagaatcgc ttccacgctc tctttaaatt tcgccggatg
    13801 cgcggtgccg aggaacaagc catattcgcc tggattcaac tggtcacgca gcgcacgata
    13861 cgctacggca gcgtgcggct ccgaggtgta gcccagttct tttaactcac gcattgtctg
    13921 ttgcgtggtt tcatcatcca cggctgcata acccagctct ttcagttgcc agattttgcg
    13981 gcggaacaac tcttccacac gcggccagtt gttcggctga ctcacgtcca tcgcgttgga
    14041 taacgtcgcc tgagtcgctt tgggtgacca ctgaccgtcg tgcaggaaac gtggcacggt
    14101 atcgttcacg ttggtcgcag caataaaacg tttcaccggc agaccgaatg acttcgccag
    14161 cagacccgcc gtcaaatcgc cgaagtttcc gcttggcacc gagacaacca gctggttgcg
    14221 cgcctcctgc ggcagctgcg caacagcttc aaagtagtag caaatctgcg ccagcaaacg
    14281 gctgatgtta atcgagttag ccgagtttaa ccccagcgcc actttcagtt cttcatcatc
    14341 aaacgcctgc ttcaccagcg cctgacaggc atcgaaatcg ccgtcgatgg caacagtttc
    14401 gatattgccg cccaacgtac agaacagttt ttcttgcagt ggactgattt tgcctcgtgg
    14461 atagaggata accactttca cattcggcaa accgtagaaa gcatgagcca ctgcagctcc
    14521 ggtatcaccg gaggtcgcgg tcagaatggt cactggctta tcatccgcaa tatgggtcag
    14581 catttgtgcc ataaagcgac cgccgaaatc tttaaatgcc agcgttggcc cgtggaacaa
    14641 ttccagacaa ccgacatcgc tttcaacatt ggcgaccgga gccgggaagg caaacgccgc
    14701 gcgcacgcgc tcttccagga tttcctgtgg gatttcatca ccaataaacg ccgagaggat
    14761 cttcgcactg cgggtgacaa aatccagctt cagcatctca tcaatttcag tcaggctgaa
    14821 ttccggcagg tcgtgcggaa aaaacagccc ctgatttttg cccaacccct gggttacggc
    14881 ttgcgcaaag ctgacctgct cgttgtgatc tttcagattg tagagtttca tttagttttc
    14941 cagtactcgt gcgcccgccg tatccagccg gcaaatatga acaaaacctt cctgattttg
    15001 caggtagttc ttacccaacc agtcggcaac gcgctgggcg gtatccggct tgtcacatag
    15061 agcgaacaag gtcgggccgg agccggagat accgctcgct accgcgccga tttccgcgac
    15121 cgcctgccgc gcctgccgga agccaggcag taaccgttca cggtagggtt cagcgataac
    15181 atctttcatc agcttcgcgg caagctcagg ctgacgggaa tagcaggcgt gaatgaagcc
    15241 agccagatgt cgcccgtgcg caatgcaatc ctggcggcga tactgcgccg gtaaaatagc
    15301 ccgggcttct gccgtcgaga ctttaattcc cggatacgcc agcacccaca gccactcatc
    15361 aaaccctggc acttgctggc tgatgatgtc gttttcttcg atcatcaact gcataccacc
    15421 aagaaaacac ggtgccacgt tgtcgtaatg aatgctgccg gagatacgtc cttccagctc
    15481 gcccatcaaa gccagcaaac gagtgtcatt aagcggcttg ccgcaatgtt cattcatcgc
    15541 catcagcgcc gcgaccaccg aacaggcgct ggagcctaag cccgaaccga tcggcatatt
    15601 cttttccaga gtcatcgcca ctggaatttg cttgccaagc tcctggcaaa aacgctccca
    15661 gcactgataa acgatatttt cccgtggttc tgacggcagc ttatcggcaa agcgtccgag
    15721 tttgttgaga ctgaatgtct ctgccgcctc aaccgtgact acatctccga gcaatgcacc
    15781 atcaacgggt gccaccgccg ccccgagcac atcaaacccg acgctcatat tggcactgga
    15841 agccggggca taaactttaa ccatgtcaga ctcctaactt ccatgagagg gtacgtagca
    15901 gatcggcaaa gacaccggca gcggtaacgt cattgcccgc accatatccg cgcagcacca
    15961 acggcagcgg ctgataatag tggctataaa aggccagggc gttttcgcca tttttcactt
    16021 tgaacagcgg atcattccca tccacttcgg caatcttcac gcggcagaca ccatcttcat
    16081 caatattgcc aacatagcgc aaaacttttc cttcatcacg ggccttcgcc acgcgcgcgg
    16141 caaagagatc gtcgagctgt gacagattcg ccataaaagc ggcaacatca ccctcggcgt
    16201 taaactctgc gggcagcaca ggttcaattt caatatccgc cagctccagt tcacgtcccg
    16261 tttcacgagc gagaatcaat agtttacgcg ccacatccat accagaaaga tcatctcgcg
    16321 ggtccggttc ggtataaccc atttcccgcg ccagcgtggt cgcctcggag aggctcatgc
    16381 cttcgtctaa cttgccgaag atataagaaa gcgaacctga aagaatgccg gagaacttca
    16441 ccaattcatc acctgcattg agcagatttt gcaggttctc aataaccggt aatccagccc
    16501 caacgttggt gtcatagagg aatttacgcc gcgatttttc cgccgcataa cgcaactgat
    16561 gatagtaatc catcgacgag gtgttggcct ttttgttcgg cgtgacaacg tggaaacctt
    16621 cgcgcaggaa gtcggcatat tgatccgcca ctgcctggct ggaggtgcag tcaacaatga
    16681 ccgggttcag cagatgatat tctttcacga ggcgaattaa gcgcccgaga ttaaacggct
    16741 ctttggcttg tgcaagttct tcctgccagt tttccagatt aaggccatgc acactagtga
    16801 gcagagcctt cgagttggca acgccgcaga cacgtaagtc gatatgttta ttcttcagcc
    16861 agctttgctg acgcttcagt tgctccagca gcgcaccgcc aacgccaccg acgccaatca
    16921 caaacacttc gataacctga tcggtattga acagcatctg atgagtaacg cgcacgccag
    16981 tggtcgcatc atcgttattt accacgacag agattgagcg ttcagaagat ccctgagcaa
    17041 tggcgacaat gttgatattg gcgcgggcca gcgcggcaaa gaatttcgcc gagatcccac
    17101 gcaaggtgcg cataccatca cctaccaccg agataatggc cagccgttcc gtcaccgcca
    17161 gcggctccag taagccttct ttcagttcca ggtagaactc ttcctgcatt gcccgttcag
    17221 ctcgcacaca gtcgctttgt ggaacgcaga aactgatact gtattcggaa gatgattgcg
    17281 taatcagcac cacggaaata cgggcgcgtg acattgcagc aaagacgcgc gccgccatgc
    17341 caaccatccc tttcatcccc gggccggaaa cgctgaacat tgccatgtta ttcagattgg
    17401 aaatgccctt gaccggtaat tcgtcttcat cacggctggc accaatgagc gtacctggtg
    17461 cttgaggatt tccggtattt ttaatcaggc aagggatctg gaactgggcg atgggggtaa
    17521 tggtgcgggg gtgaagaact ttagcgccga agtaggaaag ctccatcgct tcctggtagg
    17581 acatcgactt caacaacctc gcatcgggca cctgacgcgg gtcgcaggta tagaccccgt
    17641 caacgtccgt ccaaatctcg caacaatcgg cgcgtaaaca ggcagccagc accgccgcgg
    17701 agtagtcgga accgttacgt ccaagcacca ccagttcgcc tttttcatta ccggcggtga
    17761 aacctgccat caacaccatg tgatcagccg gaatgcggct tgccgcaata cggcgggtgg
    17821 actcagcaat atcgacggta gattcgaggt aatgccccac tgccagcagt ttttcgaccg
    17881 gatcgataac ggtaacgttg tgaccacgcg cttctaacac gccggccata atggcgatcg
    17941 acattttctc gccacggcaa atcagcgcag cgttgatgct atccgggcac tgccccaaca
    18001 aactaatgcc atgcaggaca tgttttattt gggcaaattc ctgatcgacg aaagttttca
    18061 attgcgccag cgggaacccc ggctgggcgg cggcgagtcc cgtcaaaagt tcggcaaaaa
    18121 tacgttcggc atcgctgata ttgggtaaag catcctggcc gctaatgttt ttttcaatca
    18181 tcgccaccag gtggttggtg attttggcgg gggcagagag gacggtggcc acctgcccct
    18241 gcctggcatt gctttccaga atatcggcaa cacgcagaaa acgttctgca tttgccactg
    18301 atgtaccgcc gaacttcaac actcgcatgg ttgttacctc gttacctttg gtcgaaaaaa
    18361 aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc
    18421 gttacctgtg gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt tcatggatgt
    18481 tgtgtactct gtaattttta tctgtctgtg cgctatgcct atattggtta aagtatttag
    18541 tgacctaagt caataaaatt ttaatttact cacggcaggt aaccagttca gaagctgcta
    18601 tcagacactc tttttttaat ccacacagag acatattgcc cgttgcagtc agaatgaaaa
    18661 gctgaaaaat acttactaag gcgtttttta tttggtgata tttttttcaa tatcatgcag
    18721 caaacggtgc aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt gctgtaacca
    18781 gtcgaccagt tttatgtcat ctgccactgc cagagtcgtc agcaatgcca tgactcgttc
    18841 gcgtaaagct tgcagttgat gttggtctgc cgttgtgtca cttttcgccg gttgttgtat
    18901 taatgttgct aattgatagc aatagaccat caccgcctgc cccagattga gcgaaggata
    18961 atccgccacc atcggcacac cagtaagcac gtcagccaac gctaactctt cgttagtcag
    19021 cccggaatct tcgcgaccaa acaccagcgc ggcatggctc atccatgaag atttttcctc
    19081 taacagcggc accagttcta ctggcgtggc gtagtaatga tatttcgccc gactacgcgc
    19141 agtggtggcg acagtgaaat cgacatcgtg taacgattca gccagtgtcg ggaaaacttt
    19201 aatattatca ataatatcac cagatccatg tgcgacccag cgggtggctg gctccaggtg
    19261 tgcctgacta tcgacaatcc gcagttcgct aaaacccatc gttttcattg cccgcgccgc
    19321 tgccccaata ttttctgctc tggcgggtgc gaccaggata atcgttatac gcatattgcc
    19381 actcttcttg accaaataac cgcgaacagg gtgatcactg tcaacttatt acgcggtgcg
    19441 aatttacaaa ttcttaacgt aagccgcaga aaaagccctt tacttagctt aaaaaaggct
    19501 aaactatttt ctgactgcac taacggttta attgttaaaa aatgctacat atccttctgt
    19561 ttacttagga taattttata aaaaataaat ctcgacaatt ggattcacca cgtttattag
    19621 ttgtatgatg caactagttg gattattaaa ataatgtgac gaaagctagc atttagatac
    19681 gatgatttca tcaaactgtt aacgtgctac aattgaactt gatatatgtc aacgaagcgt
    19741 agttttattg ggtgtccggc ccctcttagc ttgttatgtt gctgttaaaa tggttaggat
    19801 gacagccgtt tttgacactg tcgggtcctg agggaaagta cccacgacca agctaatgat
    19861 gttgttgacg ttgatggaaa gtgcatcaag aacgcaatta cgtactttag tcatgttacg
    19921 ccgatcatgt taatttgcag catgcatcag gcaggtcagg gacttttgta cttcctgttt
    19981 cgatttagtt ggcaatttag gtagcaaaca tgcagacccc gcacattctt atcgttgaag
    20041 acgagttggt aacacgcaac acgttgaaaa gtattttcga agcggaaggc tatgatgttt
    20101 tcgaagcgac agatggcgcg gaaatgcatc agatcctctc tgaatatgac atcaacctgg
    20161 tgatcatgga tatcaatctg ccgggtaaga acggtcttct gttagcgcgt gaactgcgcg
    20221 agcaggcgaa tgttgcgttg atgttcctga ctggccgtga caacgaagtc gataaaattc
    20281 tcggcctcga aatcggtgca gatgactaca tcaccaaacc gttcaacccg cgtgaactga
    20341 cgattcgtgc acgcaacctg ctgtcccgta ccatgaatct gggtactgtc agcgaagaac
    20401 gtcgtagcgt tgaaagctac aagttcaatg gttgggaact ggacatcaac agccgttcgt
    20461 tgatcggccc tgatggcgag cagtacaagc tgccgcgcag cgagttccgc gccatgcttc
    20521 acttctgtga aaacccaggc aaaattcagt ctcgtgctga actgctgaag aaaatgaccg
    20581 gccgtgagct gaaaccgcac gaccgtactg tagacgtgac gatccgccgt attcgtaaac
    20641 atttcgaatc tacgccggat acgccggaaa tcatcgccac cattcacggt gaaggttatc
    20701 gcttctgcgg tgatctggaa gattaatcgg ctttaccacc gtcaaaaaaa cggcgctttt
    20761 tagcgccgtt tttatttttc aaccttattt ccagatacgt aattcatcgt ccgttgtaac
    20821 ttctttactg gctttcattt tcggcagtga aaacgcatac cagtctatat tacgagtcac
    20881 aaacatcatg ccggccagcg ccaccaccag cacactggtt cccaacaaca gcgcgctatc
    20941 ggcagagttg agcagtcccc acatcactcc atccagcaac aacagcgcga gggtaaacaa
    21001 catgctattg cgccagcctt tcaataccgc ctgcaaataa ataccgttca ttaacgcccc
    21061 aatcagactg gcgattatcc atgccacggt aaaaccgata tgttcagaaa gcgccagcaa
    21121 gagcaaataa aacatcacca atgaaagccc caccagcaaa tattgcattg ggtgtaaacg
    21181 ttgcgcggtg agcgtttcaa aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat
    21241 ggcgtactta gtcgcccggt ccgttaattg gtattgatcg gctggcgtcg tcactgcgac
    21301 gctaaacgcg gggaagtttt cccagccggt atcattgcct gaagcaaaac gctcaccgag
    21361 attattagca aaccagctgc tttgccattg cgcctgaaaa cctgactcgc tagcttcccg
    21421 tttggctggt agaaaatcac ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat
    21481 ttcgctatta cgcccggcag gcaccacaga aagatcgccg gtaccgctta aattcagtgc
    21541 catattcagc tgcaggttct gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc
    21601 ctgcccgcct tgctctaacc cggtaccggg ttcaatggtc agtgccgttc cgttaacttc
    21661 aggcgctttc accacaccaa taccacgcgc atccccgacg ctaatcacaa taaagggctt
    21721 gcctaagatg atatttggcg cgttgagctc gctaagacgc gaaacatcga aatcggcttt
    21781 tagcgttaaa tcactgtgcc agacctgacc ggtataaatc cctatcttgc gttcttccac
    21841 gttctgattg ccatcaacca tcaatgactc aggtaaccaa aaatggataa aacttcgttt
    21901 ccgctccacg gttttatcct cttcctgcac cgtataaagc tcggtcacag gaatagcgat
    21961 aagcggccca acgagttttt gcggcccgct ggtactttgg cgaatcgcat cttccacatc
    22021 gctacggtaa tcggcacgtt cgacaatcac ttgccgaatc agcattatcg gaatcaacaa
    22081 cagcaatact gcaccaaaca aggtggtcat tttccagaac aggggggatt tcaacatagt
    22141 cttctccttt gcaatggcag caggatacga aggctatgtg ggaagaattt gaagctatgt
    22201 gaagtgacgg tgaagtcgta gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt
    22261 gacttcgccg ttaaacaaac gggcgacctc actgacgaac gccaacccca gaccgctgct
    22321 tttttgccca tttgcacgcg gcaaagagta aaagcgttca aaaatacgtg acagcgcgta
    22381 gtcaggaata ccactaccgg tatccagcac cttaagcgtg acgtgttcct gatccacttc
    22441 ggcgcttagc gttatgcaac cgctctcggg agtaaaatcg atggcgttat ccagtaaatt
    22501 ccccagcgcc tgatccagta acgccggttc agcagcaaca ttaacctcgg tgggcataac
    22561 atgcaaagtg atgttttttt ctgccaactg cacggtgcgc gcttcgctga cgcggcgaaa
    22621 taatgccgcc acatcaacag cagtcagaac gacttcctga cgattctcca gtcttgcctg
    22681 gcgtagtaac gtttccacca gcgcctgcat tcgcgcattt tgtgtcagaa tgttgtcagt
    22741 aaaacgagcc actacttcag gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat
    22801 cgccgccagt gggcttttta gctcatgggt taacgcataa acatactgct caatatagtt
    22861 tttcccttcc agcttcacgc gcatactttc cagcgcctgc gcgagtttac gcagctcgct
    22921 actaccgaga tcgggaagag gaacgggctt attgtcagtg acggaatcag catagcgagt
    22981 gagccgggca atggagcggt tgatccacca aaccatgcct gcgccaatca ccagtgcaat
    23041 ccccaacaaa atggcgctgg cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc
    23101 catcgccgcg ttcggtttgc ctacgctcaa aacgccaata agccgcgagc cgcccataat
    23161 cggcgcggca acatacatca ccgaactttc gggatcggca ggattttgca acgtgctgcg
    23221 cgcaccatac tgaccacgca acgttagcca gacgtcattc cagcgcgaat aatcctgtcc
    23281 aacggcttta tttgccgaat cgaacaatac tttgccatgc gcatcggtca tatagacatg
    23341 atattcattg cgcactttgt taatgccacc aatattggcg cgaaacgggc gatgttgtag
    23401 ctgattaaac gcctgcgcca gttgcccatg cgttgggtcc ccagagagca aatcgggacg
    23461 cgccagctcc gcaagtaacg ttgccgtgtc gatcaacgtc ccctccgttg ctcttcgcac
    23521 gcccggttta acttctttga caaaaatagc cagtacgaac caggccgcca ccgccaccag
    23581 taaaaaatag cccagcagca accgcatgcc gatacgcatt acaagcccct caggctatat
    23641 cccatgccgc gatgagtatt aatcggtgaa agatcggggt tgatggcgcg cagcttggca
    23701 cgcagcgttt taatgtggat atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac
    23761 acgctatcca ttagttgctg gcgggaccag acgcggcctg gcgacttgag taacgtcttc
    23821 agcaataaaa actcataccg cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc
    23881 gcgggttcat tcaattcaaa atgaccaata cggatgacgg gagacggcgt cgagaacttc
    23941 ttcacccgac gcagtaaggt gcgcaccctg gcgcacactt cgcggggtga aaacggttta
    24001 gccacatagt cgtcagcgcc aatttccagc ccaagcaggc gatcgacctc ttcacttcgg
    24061 gccgtcagga acagtacagg taacgccgga tggagcgcca gtaactggcg gcacaattca
    24121 aagccactga tatcaggcaa accaacgtcg agaatcatga cgtcgggaac ctgctggcga
    24181 gctttatcca gcaccggcaa acctcgctca aagacctcga cggcaaaacc ttcctgctgc
    24241 aacatgtaga ccagcgtgtc ggctatccct tgctcatctt ccactaacca gaccgtttcc
    24301 cgttgcataa atcgcctctg ttattgccgc cacggcatga caggaaccgc gctaatcgcg
    24361 tttttcggcg aaccttctac aactttgtcg gagtaagcca gataagcgag cgcgttgcgt
    24421 ttggcgtcat aaaagcgcac gacctgtaac gacttaaaga ccagggacgt gcgttttttg
    24481 aatactacct cgccctgagc tttgccgttt ttaatacgat ccgacagttc aatcggcccg
    24541 acttgctgac aagaaatggc cgcatcggag gtatcttctg ccaggcccaa tcccccttta
    24601 ataccaccgg ttttcgcccg gctcacataa caggtgacat ttttcacatc gggatcgtca
    24661 aaggcttcca caacaatttt gtgatccggg ccgatcattt taaatacggt gtcgaccgaa
    24721 ccaatctctt ctgcatgagc caatggcccc agcattatca ggcttaaaga caggatcaaa
    24781 tgcttgtatt tcatattgtt accattttta tctattacac gagcaattag ttcgtcacgg
    24841 taaataacat attgattaag aacaaaatct caattctttt atttccgtca agccctaaaa
    24901 catactctac taagaaaaaa acactgaatg ctaaaacagc aaaaaatgct attatccaat
    24961 tacctgatgt caggtgctcg ttgttgaaag gatgaggata ttttatggat caggccggca
    25021 ttattcgcga ccttttaatc tggctggaag gtcatctgga tcagcccctg tcgctcgaca
    25081 atgtagcggc gaaagcaggt tattccaagt ggcacttaca gagaatgttt aaagatgtca
    25141 ctggccatgc tattggcgcg tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac
    25201 tacgcctgac tgcgcgtccg attctggaca tcgcgctgca ataccgcttc gactctcaac
    25261 agacatttac ccgcgcattc aagaagcagt ttgcccagac tcctgcactt taccgccgtt
    25321 ctcctgaatg gagcgccttt ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc
    25381 cagagcacaa atttgtcacc ctggaagata cgccgctgat tggtgttacc cagagctact
    25441 cctgttcgct ggagcaaatc tctgatttcc gccatgaaat gcgttatcag ttctggcacg
    25501 attttctcgg caacgcgccg accattccgc cggtgctcta cggcctgaat gaaacgcgtc
    25561 cgagtcagga taaagacgac gaacaagagg tattctatac caccgcgtta gcccaggatc
    25621 aggcagatgg ctatgtactg acggggcatc cggtgatgct gcagggcggc gaatatgtga
    25681 tgtttaccta tgaaggtctg ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa
    25741 cgtgcatgcc aatgctcaat ctgacgcgcc gtaaaggtca ggatattgag cgatactacc
    25801 cggcagaaga cgccaaagcg ggagatcgcc caattaatct gcgctgtgaa ctgctgattc
    25861 ctatccgccg ttaacgctgc agctcatcta atgcaggggc gtccagatgc gagatgtccc
    25921 ctgcggtttc aacgacccag ccagacgcca gccacaggct ttcctgataa tccacgcgcg
    25981 aaatagaaca gttacgcaga cgtaagcggc gctctgccca tgctggtaat ccgagaatcg
    26041 tactcaccag acatcccagt gcaataccat ggctcaccag cagcggtcgg cttccctgcg
    26101 gtaagtcccg gcaagattcc agtgccgcat tgacgcgatc gctgagctcc tgcattgact
    26161 cgccttcagg aatacgcccg tcaacggtgc cattgaccag ctgccgacgc cagttctctt
    26221 cttcttcggt cagagaatcg atatgtcttt tttccagcac acccatgttt aattcacgca
    26281 ggcgagaatc aaagatgatg tcacagccgc aggcctgggc gatgatttcc gccgtacgcc
    26341 gggtgcgtcc taaatcgcta ctgataatat gcgtaatgcc aagctctttg gcacgggttg
    26401 ccacctgcat cgcctgttgc tcacctttgg cggtcagcgg gctgtcagac tggccctgaa
    26461 tacgtcgctc ggcgttccac tgcgtttcac cgtggcggac taggtatacc tgtaacatgc
    26521 tttttttccg tatactgctg taacgttaat tttctgagta atgctgatta tgcaccaagt
    26581 tgtctgtgcg accaccaatc ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat
    26641 cttcggcgaa ggatcctgcc atattgcatc cgttgccgtc gagagcggtg taccggaaca
    26701 accctttggc agtgaggaaa cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg
    26761 tttacttcca gaggctgatt tttgggtggc gattgaagct ggcattgatg gcgacagcac
    26821 tttcagttgg gtggtgattg aaaacgccag ccagcgcggc gaggcgcgtt cggcaacttt
    26881 gccgttacct gcggtgattc tggaaaaagt tcgtgaaggc gaggcgcttg gtcccgtgag
    26941 tcgcgttata ccggtattga tgagattggt cgtaaggaag gggcgatcgg cgtgtttacc
    27001 gccgggaaac tcactcgcgc cagcgtctat caccaggcgg tgatcctggc gctgagtccg
    27061 tttcacaatg ccgtgtatca ggcattgtag gacggataag gcgttcacgc cgcatccgac
    27121 attcggtgca cgatgcctga tgcgacgctg ccgcgtctta tcaggcctac aaaatcaatc
    27181 gcttttcagc aacacctctt ccagccactg acgcagctcc acgggcgcgg ctttcaggct
    27241 gttagatcca cgcgtaatcg tcgcgatgcc cgcgccgagt tcatttttta actcacgctg
    27301 actcatttcg ccacgcaaca gctcttcgac aatacgcacg cgagtcccca acgcttcgcg
    27361 ctcatctggc gtcagcatca ggtttaacaa cggtaaatgg agatcgtttt ggtaggcatt
    27421 cttaagcagg tcgacaaaac gtaaccactc ctggtgacgc tgttctgcca tcgctgctga
    27481 atagggtgat tgttgggcca taatatgtcg ccattgttag cgggggaagc aaaatgactc
    27541 ccccggttgt actcgctaaa gagtacgata gcatatcata aacgtgcgga tcagtaacga
    27601 cgtccccatt ccgtggcgct catcaacgtc ggtttatccc ccatgaaata gcggtagtaa
    27661 gcatcataag ccagcacgtt cttcacataa ccgcgcgtct cggagaacgg aatactctcg
    27721 acaaatgcca cagcgtcgat acgcccggcg ctgttgccaa gccaggttcg cacccgtcca
    27781 ggtccggcgt tataagctgc tgaggagaaa atacggttat tgccaaactg ctgataaaca
    27841 tattgcaggt aactggtgcc aatgttgatg ttcgtttccg gatccagcaa ttgcccaggg
    27901 ctgctataac caggaataga gaacatcttc accgtatggg tcgctgtacc aggcataatc
    27961 tgcatcaggc cgctggcccc taccggtgat ttcactttcg gattccaggc gctctcctga
    28021 cgggcaatcg ccatcgcata gctttgcggg atctctttcc cactggtgta acgtttgaaa
    28081 agatcgttgt aagccagcgg gaatcgctct tccagatgat cccaaagctt cccggcgatc
    28141 gttgcctgaa cgctaagatc ccaccattgg ttattgaaag cataccgagc cagttgagcc
    28201 tgctctgttt ttgacttgct cttcaccaga ttggcccact cgctacgcgc ggtattatcg
    28261 agattccagt acatcaactc gcgcacgcgc gccatctccg gcccctgagt cagggtgctg
    28321 tcaacattct gcggcgcttt atcaatcttc agctcatact cttcaccgat gcgttgtgct
    28381 gcaaccatcg ggtagaaacc acgctgttgt atgagttgat gcaaaatctc tttcgcttca
    28441 gcttcacgtc cgcgttccag caataaatcc gcctgccagt aacgccattc atctttctct
    28501 tttgcttcca tcggcagacg cgccagccag gtattcaggc cgcgacgatc gccggtgcca
    28561 agcgccattc gcacacggcg ttcaatcaac gacgtggatt gcgagcgcat aatggcgtca
    28621 tcgcgccatt tcgcctgctc atcggtgaca tcattgccca tcaaacgcca ggcgacgata
    28681 tcgcgcagct cctgaatctg atcttcatta agctgctgcg cctgggcaag cgacgggatc
    28741 atcagccgtg cattttcggc atcctgccgc gccacactgg caaacgccac cgccgccatt
    28801 tgacgggtaa aatcggtcgc gccggttgta cgcgcgaagg tcagtaccgt attagggttg
    28861 ttcgccagtg aaatgattgc cgaggcgata gtctggtaat cggcaggcat ctgccctgcc
    28921 agcactgtta ccaggcctgt gttacccgct ttcatcgcca gacggatacg ctctaaatac
    28981 gccagcggat cttgtttacc tgacgcacgc cagacgctaa ataacttgtc acaggcgtta
    29041 ggctggctct tgccggttag ccacagctct ttcgcccctt gccaggcttc ttcactctgc
    29101 ccggtgttcc atttcgcata gtagtaatta cattgcgctt cggtagttcc gggcttttcc
    29161 gggctaaagg ctaacaagcc acgccagtct tcacgccgcg ccagttcatt gacgaaacga
    29221 gattgcagcg tgcgagcggg aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc
    29281 accgccggtt gattcatcag gtcatcggtg atctggcggt attccaggta gggataaagc
    29341 ggataatcct tcagtccagg catcatttgt tcgaccacat ccatctgtcg attatcccag
    29401 gcctgcttga tttgcgcgta acggctacgc tgctcatcca gtgagtcggc tcgcgccacg
    29461 ctgctgaccg tcagcagaca gacaccggca gccaacagcc gccaggtaac ttgtttggct
    29521 ttttccacaa gcgcatcctc taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata
    29581 gatttatgct aaccagtcat tgccgtttac gccacgttac ggacactttt ttacttttac
    29641 tgcgaaggtg atcgaccaac tgggattagg cggggaaaca ggctacactt cgcctttgaa
    29701 aactcatcat cacgataaac atagaggcga agtccaacgt ggctcaattc gtttatacca
    29761 tgcatcgtgt cggcaaagtt gttccgccga aacgtcatat tttgaaaaac atctctctga
    29821 gtttcttccc tggggcaaaa attggtgtcc tgggtctgaa cggcgcgggt aagtccaccc
    29881 tgctgcgcat tatggcgggc attgataaag acatcgaagg tgaagcgcgt ccgcagccag
    29941 acatcaagat tggttatctg ccgcaggaac cgcagctgaa cccggaacac accgtgcgtg
    30001 agtccattga agaagcggtt tcagaagtgg ttaacgccct gaaacgcctg gatgaagtgt
    30061 atgcgctgta cgccgatccg gatgccgatt ttgacaagct ggccgctgaa caaggccgtc
    30121 tggaagagat cattcaggct cacgacggtc ataacctgaa cgtacagctg gagcgtgcgg
    30181 cggatgcgct gcgtctgcca gattgggacg cgaaaatcgc taacctctcc ggtggtgagc
    30241 gtcgtcgcgt ggcgttgtgc cgcctgctgc tggaaaaacc agacatgctg ctgctcgacg
    30301 aaccgaccaa ccacctggat gccgaatccg tggcgtggct ggaacgcttc ctgcacgact
    30361 tcgaaggcac cgtggtggcg attactcacg accgttactt cctcgataac gttgcaggct
    30421 ggatcctcga actggaccgc ggtgaaggaa ttccgtggga aggtaactac tcctcctggc
    30481 tggagcagaa agatcagcgt ctggcgcagg aagcttcaca agaagcggcg cgtcgtaagt
    30541 cgatcgagaa agagctggag tgggtacgtc agggaactaa aggccgccag tcgaaaggta
    30601 aagcacgtct ggcacgcttt gaagagctga acagcaccga ataccagaaa cgtaacgaaa
    30661 ccaacgaact gtttactcca cctggaccgc gtctgggcga taaagtgctg gaagtcagca
    30721 acctgcgtaa atcctacggc gatcgcctgc tgattgatga cctgagcttc tcgatcccga
    30781 aaggggcgat cgtcgggatc atcggtccga acggcgcggg taaatcgacc ctgttccgta
    30841 tgatctccgg tcaggaacag ccggacagcg gcaccatcac tttgggtgaa acggtgaaac
    30901 tggcatcggt tgatcagttc cgtgactcaa tggataacag caaaaccgtt tgggaagaag
    30961 tttccggcgg gctggatatt atgaagatcg gcaacaccga gatgccaagc cgcgcctacg
    31021 ttggccgctt taactttaaa ggggttgatc agggtaaacg tgttggcgaa ctttccggtg
    31081 gtgagcgcgg tcgtctgcat ctggcgaagc tgctgcaggt tggcggcaac atgctgctgc
    31141 tcgacgaacc aaccaacgac ctggatatcg aaaccctgcg cgcgctggaa aacgccctgc
    31201 tggagttccc gggctgtgcg atggttatct cgcacgaccg ttggttcctc gaccgtatcg
    31261 ccacgcacat cctggactac caggatgaag gtaaagttga gttcttcgaa ggtaacttta
    31321 ctgagtacga agagtacaaa aaacgcacgc tgggcgcaga cgcgctggag ccgaagcgta
    31381 tcaagtacaa gcgtattgcg aagtaatgcg taaaatgccg gatgcggcgc gaacgcctta
    31441 tccggcctac aaaaccagca atttcaatac gttgtaaaac tgtaggcctg ataagacgcg
    31501 gtaagcgtcg catcaggcat tgagcaccga atgccggatg cggcgtgaac accttttccg
    31561 gtctacaaca gccttagcat cctcaatttc gtggcgggtc acccgtgcag gttttttcaa
    31621 accttgttta atctcgacag cctcttccag agaggttttc aactcatcaa aaaaactcat
    31681 tttatacctc gcctttgagc agtttcgtca cggttatcct tgctccccca tcatctcccg
    31741 caccagctcc acgcagcgca ggaaacggct gtcgtaatcg tcctcttcaa cccgcacgaa
    31801 ttcgatattg ttctcttcca gcatctccac cagcaagttc tggaactctt tgcgatccac
    31861 cgaactgccg aggctgcgta aaccatccgc cacccacggc gtgttgttct ccagcaggat
    31921 caccagatcg aaacggtatt catcaatcag cgcctgcacg aacggatgct cacgcccttc
    31981 gtactttttg cagaacgcct gggtagtgac aaaatcggtg tcaataaacg ccactttatt
    32041 ggcgtatttc actgcaaaat caatgtattg tgcgtgaccc aacgcgattt tatcgtagtc
    32101 ggaatactgc aatgcgatct catcaccgcc gaggtgtgaa aagacataat cgcggccata
    32161 ttcccacgca ctggtggtgt tgaagatatt ggcaagtttg tttaccaggg tggatttacc
    32221 gctcgactcg ccgccaagga tcgccacggt acgcacaaaa aacggcttca cttcggtagg
    32281 aatatattcc cagtagcgga acgggttttc gcggatctgc gcaccgctga tactcataaa
    32341 ggtacgtttc ggatcgacca gcaccgtctc gatccccaga tgttccatat actgtggcgc
    32401 atcggcttct tccgaggtgt agatcaggtc cggctggatc cccttttcag ccataaactt
    32461 tttgatgccg ttgctccaca catcccagcc atgcggatac ggctccatgc cctcttcgtt
    32521 gaaagcatga atacggatat ttttctgata tttaaaggtt tgcaatagcc agcgcagacg
    32581 atccggcacc gtgggctgct gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt
    32641 atcgtcaaaa cccataatga tatgcagctc gtcaacctgg ctacaggcgc gctggataag
    32701 gtagatatgt ccggtatgca gcgggtagaa cttaccgaat acgacaccaa tggttttctt
    32761 ctgccgggga aattcaagcc ccaaaaaacg gtgcaatgcc tccagctttt gcgcgctggg
    32821 gcttttaatt ttggcattca gtaactggct taaataccct ttggtcatac cgctggcatc
    32881 agctacctgc tgtagcgtgc agccctgttg cttgatggca gttttcaggt aatcaaatga
    32941 cgacatatct ccctccgtat ctctcattat aagtcgtcga acacgctaag cgcgtcggag
    33001 agttttttaa cgccaaaaat ctgcatccct tccggcgctt ttttcggtac gttagccgcc
    33061 ggaacaatcg cccggcgaaa accgtgtttc gccgcttcag aaatccgctc ctggccactg
    33121 ggcaccgggc ggatctcccc tgccagcccg acttcaccaa acaccaccag atcctgtggc
    33181 agcggtctgt cacgcaggct ggaaaccatc gccagcagta acgctaagtc ggcgctggtt
    33241 tcggttacct tcacgccgcc gaccacgttc acaaacacat cctgatcggc catttgcaga
    33301 ccaccgtgac ggtgcaagac agccagcagg attgccagac ggttttgttc cagccccact
    33361 gccacgcggc gcggattcgc cattatcgag tgatcgacca gcgcctgaat ctccaccagc
    33421 aacggacgcg ttccttccca caccaccatc acggagctgc cggaggtcac ttcatctccg
    33481 cgacttaaga aaattgccga agggttgctg acttcacgca gcccctgttc ggtcatcgcg
    33541 aagacgccca gctcattcac cgcgccgaag cggtttttat ggctgcgtaa ggtgcgaaaa
    33601 cgggagtcgg catcgccatc cagaagcacc gaacagtcga tacagtgttc cagcactttc
    33661 gggccagcca gcgaaccatc tttggttacg tgtccaacca tgacaatcgc cacaccgcgc
    33721 gttttggcga aacgcgtcag ataagccgcc gtttcacgca cctgcgccac gctaccaggc
    33781 gatgattgta catccgccat atgcatcacc tggatcgagt caattaccat cagcttcggt
    33841 tgctcttctt cggcaatcag gcagatctgc tcgatgctgg tttccgacaa catattgaga
    33901 ttgtcagtcg gcaggccaag gcgatgagcg cgcattgcca cctgttgcag cgactcttcg
    33961 ccggtgacat acagcgtttt catctgctgg gccagtttgc acagcgtttg cagcaacagc
    34021 gtggatttcc ccgcaccagg gttaccgcca atcagaatgg cacttcctgg caccacgccg
    34081 cctcctagta cgcggtcgaa ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg
    34141 ctgatatcgg agagtttctg gacttttgcc accccggcgc taccggcata gccgctgaga
    34201 cgctcgttac gcgccaccgt tggcgacgca gcaagacgca cctcggtgat ggtgttccag
    34261 gcatgacagg cactgcactg cccctgccag cgcggataat cggccccgca ttcattacaa
    34321 acaaaggcgc gttttggagc ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca
    34381 ggatgacggg caggcaatta cttctgattc aggctgccag agaggatgca gaataccccc
    34441 atcaggtcag cgtgacggat ggtgacttcc gccttttcat tcacttttgg cttggcatgg
    34501 taggcaatcc ccagccctgc tgctttgatc atcggcaggt cattagctcc atcgccaatc
    34561 gccacggtct gcgccagcgg gatttcatac tcctgcgcga ggcgagtcag agttttcgct
    34621 ttgtactgcg cgtctacgat gtcgccgatc acattgccgg taaatttacc gtccatgatc
    34681 tccagttcat tggctaccac ggctgtcagg cgcagcttgt cgcgcaggta ttcagcaaag
    34741 aaagtaaagc cgccggaggc aatcgccact ttccagccca gcgtttccag cttgagcacc
    34801 agctgcgtta agcctggcat cagcggcaga ttttcacgca cctgttgcag aatattggcg
    34861 tcagcgcctt tcagcgtcgc cacgcggctg cgcaggctgg cggtaaaatc gagttcgccg
    34921 cgcatcgccc gttcggttac ctccgccacc atctcgcccg ttccggccag cttggcaatt
    34981 tcatcaatac attcaatctg gatggcggtg gaatccatgt ccatcaccag caaacccggc
    35041 gtgcgcaggt gcgggatttt ccccagcggg gcgacatcca gctgcgcttc gtgggccagg
    35101 cgtgtagccc gtgcggtgag tgaacctgcc agacgaatca cctgataatc ttccacgcac
    35161 caggcggcaa caatcaccat cgccgcaccc agtttgctct ggtattgggt cagacgctgt
    35221 ttatccagcc cacgaccata cagcagccag ccgctacgac ctgcgtggta atccagtggc
    35281 atcacttcat caccacttaa tgaaagaggc agacccggcc ataaagagac atcttcaggc
    35341 aggtcgcacc aggtaatgtt aggcattaag gctcctgtaa aatcgttcga agcagggaaa
    35401 ataacgcatg aggctacctt gtatccattg cttctggcaa cattaagtct caaattttca
    35461 aagggtggaa gatggctcgc acaaaactga aattccggct acatcgggca gtgattgtcc
    35521 tgttctgtct tgccttgtta gtggcactga tgcagggggc gtcatggttt agtcaaaacc
    35581 accagcgaca gcgtaatcca cagctggaag aactggcccg caccctggcg cgtcaggtga
    35641 cgctgaacgt tgcaccgctg atgcgtaccg actcaccgga tgaaaaacgc attcaggcga
    35701 tcctcgatca gttaacagat gaaagccgta tcctcgacgc gggtgtgtat gacgaacaag
    35761 gcgatcttat cgcacgttct ggcgaaagcg tcgaagtgcg cgatcggctg gcgctcgacg
    35821 gtaaaaaagc aggcggctat tttaaccagc agattgtcga gccaattgcg ggtaaaaacg
    35881 gaccgctcgg ctatctgcgc ctgacactcg acacccatac gctcgccacc gaagcccaac
    35941 aggtggataa caccactaac attttacgcc tgatgttgct gctctcactg gcaatcggcg
    36001 tagtgctgac ccgcacgctg ctacagggta aacgcacccg ctggcagcaa tcgcccttcc
    36061 tgttaaccgc cagcaaaccg gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca
    36121 ttactacaag aaaggaaatc gttatgtcca cattacgcct gctcatctct gactcttacg
    36181 acccgtggtt taacctggcg gtggaagagt gtatttttcg ccaaatgccc gccacgcagc
    36241 gcgttctgtt tctctggcgc aatgccgaca cggtagtaat tggtcgcgcg cagaacccgt
    36301 ggaaagagtg taatacccgg cggatggaag aagataacgt ccgcctggcg cggcgcagtg
    36361 gcggtggcgg cgcggtgttc cacgatctcg gcaatacctg ctttaccttt atggctggca
    36421 agccggagta cgataaaact atctccacgt cgattgtgct caatgcgctg aacgcgctcg
    36481 gcgtcagcgc cgaagcgtcc ggacgtaacg atctggtggt gaaaaccgtc gaaggcgacc
    36541 gcaaagtctc aggctcggcc tatcgcgaaa ccaaagatcg cggcttccac cacggcacct
    36601 tgctactcaa tgccgacctc agccgcctgg caaactatct caatccggat aaaaagaaac
    36661 tggcggcgaa aggcattact tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc
    36721 cggggctcac catgagcagg tttgcgaggc cataaccgag gcctttttcg cccattatgg
    36781 cgagcacgtg gaagcggaaa tcatctcccc ggacaaaacg ccagacttgc caaacttcgc
    36841 cgaaaccttt gcccgtcaga gtagctgaga gtggaacttc ggtcaggctc cggcattctc
    36901 gcatctgctg gatgaacgct ttagctgggg cggcgtggaa ctgcatttcg acgttgaaaa
    36961 aggccatatc acccgcgccc aggtatttac cgacagcctc aaccccgcgc cgctggaagc
    37021 cctcgccggg cgactgcaag gctgcctgta ccgcgcggat atgctgcaac aagagtgcga
    37081 agcgctgttg gttgacttcc cggaccagga aaaagagcta cgggagttgt cgacgtggat
    37141 agcgggggcg gtaaggtaac tcgctgatta aaatatccaa ggaaggaaat atgatcgatc
    37201 tggaaaacca ggaacgggag attatcaatt taatgttcag ccagcgtatt tcctggctag
    37261 ccgccgtgcg tatcaggcat aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct
    37321 cgattaattc tctaaaacag atcgaaaaga cggaacggct tagctcaaac atcaaaagca
    37381 aaatggcaga aatttatggt tgtccaccag agttgctgat atgcccttcc tggatgacgg
    37441 ctgaacataa atgaatagat tcgtgaatag atacttgaat gttttttatt ttctatccat
    37501 atgaaaaata atagtaaatc tataaatcag ttgctattga tgaatagata ctaaaaagcc
    37561 ggagcagtcc cccactccgg cttaacacga caatcgcctt tcagcgaaac acaattactc
    37621 tttatcgccc agcagaacgg attccagtgc gattttgatc atgtcgttga aggtggtctg
    37681 acgctcagcg gcagtggtct gctcgtgagt gcggatgtgg tcagacacgg tgcagatggt
    37741 cagggctttc gcgccaaact ctgcagccac gccgtagata ccagccgctt ccatttccac
    37801 gccgaggatg ccgtattttt ccatcacgtc gaacatttcg ccgtccggag agtagaacag
    37861 gtcagcggag aacaggttac ccacgcgagc atcaacgccc agtgctttag ctgcatctac
    37921 tgcgttacgc accatgtcaa agtcagcgat agcggcaaag tcatggtctt taaaacggat
    37981 gcggttaact ttggaatcgg tgcaggcccc cataccgata acgacgtcgc gcagttttac
    38041 gtgcggcaga actgcgccac aggaaccaac gcggataatt ttcttcacgc cgaaatcggt
    38101 gatcagttct ttggtgtaga tggagcagga cgggataccc ataccgtgac ccattacgga
    38161 aattttgcgg cctttgtaag taccagtgaa gcccagcata ccgcgaacgt tgttcacttc
    38221 acgggcatct tcaaggaaag tttcagcaat atacttcgca cgcagcgggt cgcctggcat
    38281 caaaactacg tcagcgaaat cgcccatttc tgcattaatg tgtggggtag ccattgtttt
    38341 atcctttttg tgacataaca aaggcggagt caacgctccg cccaaatcca tcagaacatg
    38401 gctttgccat attccatatc agaagtacca aaatattttg ccagagtctg gccgatatcc
    38461 gcgaaggttt cacggtgacc cagtgagccc ggttttactt tcgggccata taccagtacc
    38521 ggaatgtgtt cacgcgtgtg gtcagtaccg gtccaggtcg gatcgcaacc gtggtcagcg
    38581 gtgaggatca ggatgtcgtc atcgcgcagc agagacatca gctccggcag acggcggtcg
    38641 aacagttcca gacccgcagc ataaccggcg acgtcgcggc ggtggcccca ggaagagtcg
    38701 aagtcaacga agttggtgaa gacgatggtg ttatcacccg cttctttcat ctctttgatg
    38761 gtggcgtcaa acagcgcgtc caagccagtc gctttcactt ttttggtgat accgcagttg
    38821 gcgtagatgt ctgcaatttt accgacagaa accacctggc cgtgtttttc atcaaccagt
    38881 ttctgcagca cggtcggtgc tggcggctca acagccaagt cgtgacggtt accggtacgc
    38941 tggaagttac cagctttgtc accgataaac ggacgagcga taacacgacc gatattgtag
    39001 ccgccgttgg tcagctcttc acgggcgatt tcgcacagtt cgtagagttt atccagaccg
    39061 aaagtttctt catggcaggc aatctggaac acggagtcag cggaggtata gaaaatcggc
    39121 ttgccggttt tcatgtgctc ttcgcccagt tgatccagaa tgaccgtacc ggaagagtgg
    39181 cagttaccga ggtaacccgg cagattagcg cgttcgacca gtttatccag cagctcttgc
    39241 gggaagctgt tttcgtgatc ggagaaatat ccccactcaa acagaaccgg gacaccggcg
    39301 atttcccagt gaccagacgg ggtatcttta ccggatgaca tttcgtgcgc ccatgcgtac
    39361 gcgccgataa cttcagcgtt gccgtccatt cccgccggaa tgaaaccggt agaaccttcg
    39421 tgtgctttcg ccagccccag acgggtcaga tttggcagat tgagcggccc tttacgacca
    39481 ttatcagctt cgcctttggc acaagcttct gcgatatgac ccagggtgtc agccccgacg
    39541 tcaccaaagc gttctgcatc ttctgtagcg ccgatgccga atgagtccag caccataata
    39601 aatgcacgtt tcatatgttc tccgtactca gtgcttcaaa tttaaatgat cagatcagta
    39661 taccgttatt cgctgatacg gcgatagaca gttggtgtgc tttccggtgc gttatcggca
    39721 agtttaattg ccgctttcac cgctttcgcc gcttcctgcc agctgttttc gtctttcgcg
    39781 aggataaccg ccagcggacg ctgaccgtct acctggtcgc ccagacgcgc catatcagta
    39841 aagccgacgc tgtaatcgat ggtgtcagat gcctgacgac gaccgccgcc cattgcaact
    39901 actgccatcc ccagcgcgcg ggtatccatt tcactgacaa aaccttcggt atcagcatag
    39961 actgctttcg tcagcatcgc tgtcggcaga tacttcgcgt agttctcaac gaagtcggtc
    40021 gggccttttt gtgccgctac catacgacca aagacttctg ccgctttacc gttgtccagc
    40081 accgcctgca atttcgcgcg cgcttcggcg tcatctttcg ccagtttgcc ggagatcaac
    40141 atctccacgc acagcgccat cgtgacatca aacagacgcg ggttacgata ctcacccgtc
    40201 aggaactgca ccgcttcacg aacttcaacc gcgttacctg cactggaggc cagtacctga
    40261 ttcatgtcgg tgagcagcgc ggtggtgcgc acgccagcgc cgttagccac gccaacaatc
    40321 gcttcggcaa gggcttcaga gagttcgtag gtcggcataa acgcgccgct acccactttc
    40381 acgtccatca ccagcgcgtc cagaccttcc gccagtttct tcgccaggat cgaggcagtg
    40441 atcagcggga tggagtccac ggttgcggta atatcacggg tcgcgtagaa acgtttatca
    40501 gccggagcca gtgaactggt ctgaccgata atcgccacgc cgacgtcttt aataatttcg
    40561 cggaaacggt tgtcatccgg gaaaatgtcg aagccaggga tggattccag tttgtcgagc
    40621 gtaccgccag tatgaccgag gccgcgaccg gagatcatcg gaatatagcc gccgcaggct
    40681 gcgaccatcg gccccaacat cagcgaagtg acatcgccga cgccgccggt cgagtgttta
    40741 tcaacaatcg ggccattcag atgcaggctt ttccagtcga gaacggttcc tgaatctcgc
    40801 atcgccatgg tcagcgagac acgctcaggc attgtcatat cgtggaagaa aatggtcatc
    40861 gcgagggcgg caatctgccc ttcggaaata gtgttgtcgc gaataccgtt gataaagaaa
    40921 cgaatttctt catcgctcag cgcatgacca tcacgttttt tacgaataat ttcttgtgcg
    40981 agaaacaagg taacctcccg aggaaaagag tatgaggcgg cttttgccgg atgcgtctac
    41041 gccttatccg gcctacgggt agatacgtct cgtaggcctg ataagcgcag cgcatcaggc
    41101 gtaaagcatc ttacttagta gctgctggcg ctcttaccgt cgccgtggcc cagcgctttc
    41161 agcaggcttg ccagcaggct ggaagcacca aagcggtagt gacgcgcatc agcccagtca
    41221 gcaccgaaca gttcatctgc gatggcgaga tatttctgcg catcttccgc agtacgcacg
    41281 ccgcccgccg gtttgaaacc aacggttttt tctacgccca tatcacggat cacttccatc
    41341 atgatgcgag cgctttccgg cgtcgcgttc acagccactt taccggtaga ggttttgatg
    41401 aagtccgcac ccgctttgat ggagatttca gacgctttac ggatcagcgc ttcgtctttc
    41461 agttcgccgg tttcgatgat cactttcagc agtacattcg ctgccgcgca agcctcttta
    41521 caggctttca ccaggtcaaa accaacctgc tcgtcacccg ccatcagcgc gcggtacggg
    41581 aacaccacgt caacttcatc agcaccgtag gcgattgccg cacgggtttc tgccagcgcg
    41641 atttcgatgt cgtcgttacc gtgtgggaag ttggttaccg tagcgatacg gatttccggg
    41701 gtgccctgct ctttcagagt tttgcgagca atcgggataa aacgaggata gatacagata
    41761 gcggcggtat tgccgaccgg agttttggcc tgatgacaca gagcaattac tttctcgtcg
    41821 gtgtcgtcgt cattcagggt ggtcaggtcc atcaatttca gtgcacgcag gctgcttgct
    41881 ttcagatcag tcatttcatt ctcctggctt gtcgccaata aaattcacct tgcgagtttg
    41941 ttagtattct aacatctact ccgcaacaca cttcgataca catcacaatt aaggaaatct
    42001 acttacaagt ttgcatcact gtaatgcgat ctggttcaaa taattcactt tcaaatgaat
    42061 gcgtcagtgg tggcaaacgc atcaggatca aagtgaacat cacgaaactt cttacaatgg
    42121 cgcatcacct tcggcataaa aggaaaagaa gatgcccact tctcatgaaa atgcactgca
    42181 acaacgttgc cagcaaattg tcaccagccc agtgcttagc ccggagcaga agcgccattt
    42241 tctggcactg gaagcagaaa acaatctgcc ttacccacag cttcctgccg aagcccgccg
    42301 cgcgctggat gaaggtgtaa tctgcgatat gtttgaaggt catgcgccgt acaaaccgcg
    42361 ctatgtctta cccgattacg ctcgttttct ggcgaacggt tccgaatggc tggagctgga
    42421 aggcgcgaaa gatcttgatg acgcactctc tctgctgacc attctttacc accacgtacc
    42481 gtcggtcaca tcgatgccgg tctacctggg gcaactggat gcgttgttgc aaccgtatgt
    42541 tagaattcta acacaaaacg agatcgatgt tcgaataaaa cgtttctggc gttacctcga
    42601 cagaaccctg ccagatgcct ttatgcacgc caatatcggc ccgtctgatt cgcccattac
    42661 ccgcgcgatc ttacgtgcag acgcgcaggt ttcaccgaac ctgaccttta tctacgatcc
    42721 tgaaatcacc cctgatgacc tgctgctgga agtggcgaag aacatctgtg aatgtagcaa
    42781 accgcacatc gccaacggtc cggtgcatga taaaattttc acaaaagggg gctacgggat
    42841 tgtgagctgt tacaactcac tgccgctggc gggtggtggc agcacgctgg tacgccttaa
    42901 cctgaaagcc attgccgagc gcagcgaatc gctggatgac ttctttacgc gcactctacc
    42961 gcactactgc cagcagcaga tcgccatcat cgatgcgcgg tgtgaattcc tctatcaaca
    43021 atcacacttc tttgagaata gcttcctggt gaaagaaggg ctgattaacc ctgaacgttt
    43081 tgtgccaatg tttggcatgt atgggctggc ggaagcggtt aacttgctgt gtgagaaaga
    43141 agggattgcc gcgcgctacg gtaaagaagc cgccgcaaat gaagtgggtt atcgcatcag
    43201 cgcgcaactg gcggagtttg tcgccaatac ccccgtgaaa tatggctggc aaaaacgcgc
    43261 catgttacac gcacagtcgg ggatcagttc cgatatcggc accacgccgg gcgcgcgttt
    43321 gccgtatggc gatgagccag atccgatcac ccatctgcaa actgtcgccc cgcatcatgc
    43381 ttattattat tccggcatca gcgacattct gacgctcgac gaaaccatca aacgtaaccc
    43441 gcaggcactg gtacagcttt gcctcggtgc ctttaaagcc ggaatgcgtg aatttaccgc
    43501 caatgtcagc ggtaacgatc tggttcgcgt taccggttat atggtgcgtt tgtcggattt
    43561 agaaaaatat cgcgccgaag gttcacgcac caacaccacc tggctgggcg aagaagccgc
    43621 acgcaacact cgtattctgg aacgccagcc gcgcgtgata agccatgaac agcagatgcg
    43681 ctttagtcag taagattatc cccttctcct gcgttgacgg gccaggcagt cgtctggctc
    43741 tgtttttgca gggctgcaat ctgcgctgca aaaactgtca caatccgtgg acgatgggac
    43801 gttgcaatga ctgtggggag tgcgtgccac agtgtccgca tcaggcgttg cagattgttg
    43861 acggcaaagt ggtgtggaac gctgtggtct gcgagcaatg tgatacctgc ctgaagatgt
    43921 gtccgcaaca tgccacacca atggcgcaat ccatgagcgt ggacgaagtg cttagccatg
    43981 tgcgcaaagc ggtgctgttt attgaaggga taacggtaag cggcggcgaa gccacgaccc
    44041 aactgccgtt tgtagtggcg ctgtttactg ctatcaaaaa cgatccgcaa ctgcgccatc
    44101 tcacctgtct ggtggacagt aacggtatgt tgagcgaaac cggctgggaa aaattactcc
    44161 cggtgtgtga cggcgcaatg ctcgatctca aagcatgggg tagcgaatgt catcaacaat
    44221 taaccggacg cgataatcaa cagattaagc gcagcatcta tttgctggca gagcgcggca
    44281 agctggcgga actgcgtttg ctgatgatcc caggccaggt ggattatttg caacacatcg
    44341 aagaactggc ggcgtttatc aagggacttg gtgatgttcc ggttcgcctg aacgcgtttc
    44401 atgcccacgg cgtgtatggc gaggcgcaaa gctgggcgag cgccacgccg gaagacgttg
    44461 agccgttggc tgatgcatta aaggtgcgcg gggtgagccg gttgatattt ccggcgctct
    44521 atttgtgaca tgccggatac ggcctacggt tcggcacaga cttgtaggcc tgataagacg
    44581 cgtcaagcgt cgcatcaggc attgtgcgcc aactgccgga tgcggcgtga acgccttatc
    44641 cggcctacgg ttcggcacaa acctgtaggc atgataagac gcgttaagcg tcgcatcagg
    44701 cattatgcgc cgactgccgg atgctgccta cgaagggcaa actacccacg aacgttaaac
    44761 accgcatacg tgttattaag caacacttcg gcaatctcat ccgcgggttc cgggcgtaac
    44821 tcgcaaagca cggcaaacac acgggcagcc tgttccgggc ggttaggctg cccctgaaaa
    44881 ccgttgagcg gcatatccgg cgcatcggtt tccagcaaca acgatgccag cggtaatttt
    44941 gcgatgacat cgcgggtttt actggcgcgt ggataggtga tagtaccgcc tacgccgatt
    45001 ttgtagccca gctgtacaaa ccgttcagcc tgttgctggc tgccagaaaa tccgtggaca
    45061 acaccagcac gcggcaaatc gtggcgttta agatgcatcg ccagtttgtc gtgcgtgcgc
    45121 cgtgaatgca ggatcaccgg cagatcgtag cgtttcgcca gtttcagttg ttcgtcgagt
    45181 aaccactgct gcctctcaaa ttgcggatcg tcgccgaaga gatccagacc gatctccccc
    45241 accgccacca ccttcgccgg acgcctttcc agcgcctgct gtagctgctc aagagacaca
    45301 tcgctatgtt tttccaacat accaggatgc aagcccaata cggcatacag cggttgataa
    45361 ttttccgcta acgcctgcac acgggcaaaa ttttccgcct ctgtcgccgg aacaatgatc
    45421 ttgcctacgc ccgcttgtgc cgcgcgttgc aggctggcct cttcatcgcc aataaaaggc
    45481 gggaaatcaa aatggcagtg agtgtcgata aaacggcaaa tcaagccaga tcctcattgt
    45541 taaatgtcgg gtcgttcgcc tgcggagcgt cgctcacttc ggcaaccagc gtatcgttgg
    45601 cgactggcgc aggcggaatg acaatcgatt ccggcgtcac cactggcacc agatggcggg
    45661 taagcggcgc tttttcagtt aatagcttgc caaccgtggc gaggaaataa cgaccgcata
    45721 aacgcccaag tttatagtct tcacgcagcg ccggaatccg actgccaagc gcgatactat
    45781 gtaatggctt cggcggataa atttcggata tccgcagctt gcccggtggt ttctcaataa
    45841 attgctgaat gtcacgatag ctggtttcat gatgctgcac cagattgacc agcggctgca
    45901 ggctactgtc acccagccag cgttccatgc gtttgaacca ctgcggcgtg tagtacattt
    45961 gtgacggcac agtgcggatg acgaccaacg ttttagcgcc ctgccttgcc gcctctttaa
    46021 ccggaatcgc atcactgatc ccgccatcca ggtagttaat gccttccagc gacactccgc
    46081 tacgataaaa gccaggtatc gcactggagg cgcgaatcac atccagccag ttttgtttgg
    46141 ttggtaaaaa gtaattcggc gcgtagtcat cctgacgaca ggcgcacata taaaacgatt
    46201 tgccgctgtc aaacaaccgc gcggcggtat ccatttgcaa cggcatctgg cttgcggtgg
    46261 cctccaccag ccagtcgaga tcgataagat ttcctccacg gacaaagcgc aatggatcga
    46321 aaaattcgcg ttttgtggta tagcgcatga tgactttgcg cgcgtaaccg ggctgattgc
    46381 agataaacgc cgagaggttc tgcgccccgg cagatgtgcc gagataaaga tcgaaaggat
    46441 taaactgcgc gcgcataaac tcatccagca cgccagccgt gaaaattcca cgctgtccgc
    46501 cgccttcgca caccagagct attcgtccag gctggaacgg ccttagcgac aacggcgcaa
    46561 tattaccaag cgttacaggt attcgctgcc ccacttctgc cttcctgttt ttgttgtgaa
    46621 agaaagacaa agtaacgcaa tttattattc gctaaaaccg caaaagccag tccgctggac
    46681 tggctttgga taaaattttc ttaatggtct gaaatctagg gtcgttttcg gcccatgaac
    46741 aaactcacta ggaacagaat aatcccgacg acaaagacaa ttttagctgc gcctgcagcg
    46801 gtaccggcca gaccaccaaa cccaagtgcg gcggcgatta acgcgataac cagaaatatg
    46861 atgccccaac gaaacataag cgtctccttt accatagtta atgtcaccgc ttgatatgag
    46921 cgtgaaaatc actcaatgcg atatttttag tgtggtgcac atcgcgcctc ccgacaaagt
    46981 tcgggaggac gaattactta gttttcagat catttttaac gcttttcaca ccatctaccg
    47041 ctttggcgat actttcagca cggtcacttt gtgcctgaga atctacggta ccggagagct
    47101 gaaccacgcc atcggtggtt tcaactttca ctttacgtga agggacgata tcgtccgcca
    47161 gcagtttggc tttgatttca ctggtggtgg cggtgtcacc cgcgtagccc ttcaccgagc
    47221 cttctttagc gtcgcgaacg tgcagtttgt cgctgacaga ggtcacgcct tcaacgcctt
    47281 tcgccacttt cactgcctct tcggcctggg cctgactttc aacgaaaccg ctcagggtca
    47341 cgactttttg atcggttttg acagagatat cggtgctctt gatgttgtca tgatccacca
    47401 gcgccgcctt cactttcgcg gtgatggcgc tgtcatccat gaaattaccg actttattca
    47461 tagagctatc gactttttgc cctgcgcttt cattggtagt ctgcgcgttg ttttccgcgt
    47521 aggcagagcc ggtcgcgacg gcagaggtca acattacagc cagcagagtt ttcgaaatct
    47581 tcagtcttgt catagtcatc gatttattcc tgtatgtttg ctcgtaattt gagcctagca
    47641 acacgaggtt gcattgctga atagggagag acttcaccct ctacagaagt caatggtcgc
    47701 catcacaaaa gcgatgggtg atgaataacg accattacag cctctgaatc agttattaat
    47761 atcggtagaa tgacaatcga cggctttaga tactaatatc tacgcattga acggtattta
    47821 acgccatcag aaatgtcatc actttgttaa atatagatca caattttgaa accgctcggg
    47881 atatcagcga aaacataagc aaaagtgaat gttttaagaa cattccgtaa gcggctaata
    47941 aggaagggaa attgacaggg cgcagcggct accgcgccct ggaggcaaga attaatgctc
    48001 gcgggtctgg tggaactgaa cgtccggata acgttcctgt gccaggcgca ggttgaccat
    48061 gctggtagcg atgtaagcga ggttatcgcc gccatcaagc gccagttggc tttcgttctt
    48121 acgcttgaac tcttcgaatt tcttcgcgtc tgcacattct acccagcggg cagtggcaac
    48181 gttgactgac tcatacactg cttcaacgtt gtattcgctc ttcaggcgcg ctaccaccac
    48241 atcaaactgc agcacaccaa ccgcaccaac gatcagatcg ttgttggaga ttggacggaa
    48301 cacctgcacc gcgccctctt cggaaagctg taccagccct ttgagcagct gtttttgctt
    48361 cagcggatct ttcaggcgga tacgacggaa cagttctggt gcgaagttcg gaataccggt
    48421 gaacttcatc atctcaccct gggtaaaggt gtcgccgatc tgaatggtgc cgtggttgtg
    48481 caggccgagg atatcgcccg gatacgcttc ttcaacgtgc gaacggtcac ccgccataaa
    48541 ggtcagcgcg tcggagatca ccacatcttt cgcagtgcgc acctggcgca gtttcatgcc
    48601 tttttcatat ttaccggaca ccacacgcat aaacgccacg cggtcgcggt gtttcggatc
    48661 catgttggcc tgaattttaa atacgaagcc ggtaaattta tcttcgctcg cttctacggt
    48721 acgggtatcg gtctgacgcg gcatcggcgc aggcgcccac tccaccaggc cattcagcat
    48781 atgatcaacg ccgaagttac ccagcgcagt accgaagaat accggagtga tttcgcccgc
    48841 aaggaacagc tctttgtcga actcgttaga cgcgccttta accagttcca gttcgtcacg
    48901 cagctgctgt gccagatctt caccaaccgc agcatcgaga tccgggttat tcagcccttt
    48961 aacaatgcgg acttcctgaa tggtgtgccc tttaccgctc tgatagagat aggtttcatc
    49021 tttataaagg tggtaaacgc ctttaaacag cttgccgcag ccaattggcc aagtgatcgg
    49081 tgcgcagcca attttcagct cgttctcaac ttcatcgagc agttccatcg ggtcgcggat
    49141 atcacggtca agtttgttca taaaggtgag gatcggcgtg tcgcgcagac gggtaacttc
    49201 catcagctta cgggtacgat cttcaacacc ttttgcggcg tcgataacca tcaggcagca
    49261 gtccaccgcc gtcagggtac gataggtatc ttccgagaag tcttcgtgcc ccggggtgtc
    49321 gagcaggtta accaggcaat cgtgatacgg aaactgcatc acagacgtag taatggagat
    49381 cccacgctgc ttttccatct ccatccagtc cgacttagcg tgctggttgg aaccacggcc
    49441 ttttactgta ccggcggtct gaatggcctg tccgaacagc agcaccttct cggtgatggt
    49501 agttttaccg gcgtccgggt gagaaataat ggcaaaagtg cggcgcttcg ccacctcttt
    49561 caaataagga gacaacgtca taattctttc ttcttgagta agcggcatcg cgccgcgctt
    49621 gttgaaaacg aaaaattgcg gctattttac ccatcaacgg gggggaggca atcagtgttt
    49681 acacaggagc tgctccagtt cgtgcaacga agaaacggtc caggtgggcg cgatgccttc
    49741 tggttgctcg cgattgtgcg cattcagcca gcaggtcgca agcccggcgt tgatgccacc
    49801 gagaatatcg gactcggcag tgtcgccaac catcagcacg cgtgaacggt caggattgcc
    49861 cgcctgttcc agcgcataat cgaaaatttt cttattcggt ttggcaacgc caacttcttc
    49921 ggaaatcacc agcaaatcga agtaatcacg caggcccgtc cgttccagac gcacttgctg
    49981 caaggcgcta aaaccgttgg tgatgatgcc gattttagcg ttgctacgaa tggcgttaag
    50041 cagagaaacc gcgcccggca acggcgtaca gatttccgcc atcgcattaa taaaggcatc
    50101 gttaagcaag cctgcttcaa ctttcagccg ttcggcccag ctctcgaaac gcccgtgctg
    50161 aagctgtaat gaagtgatcg cgccgttttg ataatccacc cacagtggct tgttaacggc
    50221 ctgatagtcc tgaaaatctt cagcggtaaa ggtgacgctg taatcaagaa acatccgctg
    50281 caggccggtg aatgagtcaa aggtaaacag cgtttcatcg gcatcaaaga aaatccagtc
    50341 ccacttcatt attccacctt gtcttacata ctgattggca acgccatgat gatggcgtct
    50401 tcgcgaccgt ccgtggtggg gtagtaattg cggcgaatcg tcgcctcgtt aaagcctaaa
    50461 ctttcgtaca gggcaatggc ggcagcattt gaggcacgga cttccagcca tagtgtcgcc
    50521 acgccgcgtt tttccagttc gtcgatcaga tgttccagca gcgccctacc cagcccctga
    50581 cgctgatagt cgggatcgac cgcaatattg aacaatgtag cttcatccag caccacttgc
    50641 gtaatcgcaa acgccgccat tttgccgttt tgcgttaact gaaagttgag ataacgctcg
    50701 ccctggttgc tggcaaaggt tttttcactc cacggaaagg cgtgggcgcg ttgttcaatg
    50761 tggtaagccg ctggtaaatc agtcgtttcg agggaagaaa tcgtgttcat atgtgcaaat
    50821 ttgttgccat aacgcggcgc gtgccgttgg gtttgcccgt aattcggtga gcgccggtga
    50881 tgccacctga gcgccttcca atgatagcgg ttcgtcagta cccaaccgcc agctgttgca
    50941 gcgactgcct tgcggcagca tcgcgatttt ttctggcgtc agttgcagca cttgatcggg
    51001 gctgacggtt aatgcgcgca gaacatcgct cactaaagga tcagtcaggg cgggaagatc
    51061 gtttgccacc atcaccagac ggacgtgtgc cgggctcgca atggcaatct ccccctgcaa
    51121 cgcgccaggg cgacgcagcg accactgggt aatgcccagt tgctgtaact gccagtctcg
    51181 tcgggatgtc atagcgaatc gctcctgttg tcaggggcgc aaatatagca aattcgtcga
    51241 taccgcgcca acatatggct ataatcgccg ccagtatcaa ttgaggagca ttccatgtct
    51301 gcatttaccc cggcaagtga agtcttgctg cgtcacagtg atgatttcga acaaagccgt
    51361 attctgtttg ccggagactt acaggatgac ctgcccgcgc gtttagatac cgcggccagc
    51421 cgtgctcata cccagcaatt ccaccattgg caggtgttaa gctgccagat gggggataac
    51481 gcccgtttta gtctggtcgc cacggcgaat gatgtcgcag attgcgatac gctgatttac
    51541 tactggccga agaacaaacc ggaagcccag ttccagttga tgaatttact ttctctgctg
    51601 ccagtgggga cagatatttt tgtcgttggc gagaaccgca gcggcgtgcg cagcgctgag
    51661 cagatgctgg cagattatgc gccgttgaat aaagtcgaca gcgctcgtcg ctgtggcctc
    51721 tattttggtc gtctggaaaa acagccagta tttgatgcgg ataaattctg gggcgaatac
    51781 agcgtcgatg gcctgacggt caaaacgctg cctggcgtgt ttagccgcga cggtctggat
    51841 gtcggtagcc agttgctgct ctcgacgtta actccgcaca cgaaaggtaa agtgctggat
    51901 gtcggctgtg gcgcgggtgt actttcagtt gcctttgcgc gccattcgcc gaaaattcgt
    51961 ctcaccttgt gcgatgtctc tgccccggcg gtagaagcca gccgcgcaac acttgcagcc
    52021 aacggtgttg aaggtgaagt ctttgccagc aacgtctttt ccgaggtgaa aggttgtttt
    52081 gatatgatca tctccaaccc gccgttccac gatgggatgc aaaccagcct ggatgcggcg
    52141 caaacgctga ttcgtggcgc ggtgcgtcat cttaatagcg gcggcgagct gcgaattgta
    52201 gcgaacgcct tcctgcctta cccggacgtg ctggatgaga catttggctt ccatgaagtt
    52261 atcgcgcaaa ccgggcgctt caaggtatat cgcgccatta tgacccgcca ggcgaagaaa
    52321 ggttgagtat ctgatatctg tcgccgcata ccgtgtcgga tgcggcgtga acgccttatc
    52381 cgacctacaa aacctgcaca ttagcccttc gtaggccaga taagacgcgc cagcgtcgca
    52441 tctggcatct gcccccgcat gcctaccaaa cctgcacgct agtttcctga tggacatttt
    52501 tccagcaatt acacatcagg cgataattaa ctattgacga aaagctgaaa accactagaa
    52561 tgcgcctccg tggtagcaat tctttttaag aattgatggt atgcgaaggt ggcggaattg
    52621 gtagacgcgc tagcttcagg tgttagtgtc cttacggacg tgggggttca agtccccccc
    52681 ctcgcaccaa aaatcacgtt gatattgctc gcactgggcg aaggtggcgg aattggtaga
    52741 cgcgctagct tcaggtgtta gtgttcttac ggacgtgggg gttcaagtcc cccccctcgc
    52801 accaaacgag gcgatatcaa aaaaagtaag atgactgtgc gaaggtggcg gaattggtag
    52861 acgcgctagc ttcaggtgtt agtgtcctta cggacgtggg ggttcaagtc ccccccctcg
    52921 caccaattat ctttacttcc tttcttgttt cttccctgtt ttgtcatccc tgattcaaat
    52981 tcattatcat cagcatgatg ccagcaataa gcgccaggca cgatggtagc aaaatgtaat
    53041 gccgcaactg tttgtgatac agcatcaccg gcgtcaatac taatcccatc agaatgacaa
    53101 tccgccactg atccggcgat aaatccgcat acatcagccc tgcaatcagc aatcccggca
    53161 gcaacactcc ccagccactg cccagcgtac gttgcaacat ggtttcatct ctttcattga
    53221 taatgaaaac caatatcata tgataatttt tatcatttgc aagccagaga aatcccttgc
    53281 tatcgagtaa acctatcgct aggattagca atcattatca tttagattac tatcacgatt
    53341 atggcctatc gttccgcacc gctctatgaa gatgtcatct ggcgaacgca tctccagccg
    53401 caggatgctg ggcttgcaca agccgtacgg gcgatgatcg ccaaacatcg tgaacatttg
    53461 ctggagttta tccgcctgga tgaacccgct ccgcttaacg ccatgacgct ggcgcaatgg
    53521 tcatcaccga atgcgctaag ttctctactg gcggtctatt ccgatcatat ctatcgcaac
    53581 caaccaacga tgatccgcga gaacaaaccg ctgatctcac tatgggcaca atggtatatc
    53641 ggcctgatgg tgccaccatt aatgctggcg ctactgacgc aggaaaaagc attagatgtg
    53701 tcgccggaac atttccatgc tgagtttcac gaaaccggac gcgtcgcctg tttctgggtc
    53761 gatgtgtgtg aagataaaaa cgcaacacca cattcgccgc agcagcgaat ggaaacgtta
    53821 atcagccagg cgctggttcc ggttgtgcaa gcactagaag cgaccggaga gatcaacggc
    53881 aaacttatct ggagtaatac cggttatttg atcaactggt atctcactga gatgaagcaa
    53941 ctgctcggcg aggctactgt tgaatcgctg cgccatgccc tcttttttga gaaaacgctc
    54001 acgaacggtg aagataatcc actctggcgt accgtggtac tgcgcgacgg cctgctggtg
    54061 cgccgcactt gctgccagcg ttatcgctta ccggatgtgc agcaatgtgg ctattgtacg
    54121 ctgaaatgaa agcactgccg gggaagtaaa cccggcatca tgcggattaa tagggatgca
    54181 acacattact tgcttcgcaa tgccgcatac ataacagaat atcttcggcc tccaacaacc
    54241 ctgcatcaga actgacgccc agtttcgaca tcacattaaa tttatgcgct cggatcgttt
    54301 tgatattacg tttaagctgc tcggcaattt gtgtcattga gtagccacgc gacataaagc
    54361 gcaatatttc tctctccgtc gggcttaacg tccggctttg gttaatgtac cactgattgt
    54421 tcagtcggtc ggtcgcctga cgtacaccat ttaatgacaa aaagagttcc tgatgaaaaa
    54481 tctccagcgt tgacgcttta cttaatacac cgtccagcgg tgatggcgac aatgaaccaa
    54541 tcagccgagc ttcaatatca tcatccacaa taactaaacg ccgggtacgc ggaaacttaa
    54601 tcgccagctc agtcaggcaa gataatccct ctctgcgctc acttctcatg gcagaaaaag
    54661 aaaaaatgac cgccgcaaac gagatccgcg acatggcatc ctgaaatgac tcctgactgg
    54721 tataggtatg gagcttataa gggttgcccg caaactttct gaataaactc tcaataccga
    54781 tactactcat gacgcatttt tctatgagtg cgacatttct cttcttaatt cggctgtgtt
    54841 ccattctgcg gcatctccat aaacagaagg caagttaagt tctcttgcac cctgcgcgct
    54901 atttatccat gcatacattt cagcgttgct gtgaattgcc agtcgacgca tcgcactgtt
    54961 tttttgggcg ctgatagttt tattactttt cttaagcaat gaagctatct ggttgatgcc
    55021 ccatccttta cctaatagcc gcagtacctt gcgctctgaa agagtgagaa tgacggagcg
    55081 atagctaaaa tcatgagttt cagtcattgc cggagaggtc agcatggcgc tgatacgctc
    55141 tgcgtgcgta ttgccggaac gtacggtctt gaccagattc tcaatgggtt caacatcaga
    55201 caataacgtc gccgtagggc acatgagcag ttctacagcc tgggaatacc aggagcgtga
    55261 caccatgaaa acccagtgaa tttcccgata ttgtgagatt aaagaataat aatgttcaca
    55321 aacagaacgt ggatcttcac tttcaccggc taaatcagca atgactaat
//
LOCUS       DAETQL010000019        54199 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.019, whole genome shotgun sequence.
ACCESSION   DAETQL010000019 DAETQL010000000
VERSION     DAETQL010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 54199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.019"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            96..395
                     /locus_tag="K0W65_001205"
     CDS             96..395
                     /locus_tag="K0W65_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3094546.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            405..929
                     /gene="ygaP"
                     /locus_tag="K0W65_001206"
     CDS             405..929
                     /gene="ygaP"
                     /locus_tag="K0W65_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBI3094547.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(976..1380)
                     /gene="stpA"
                     /locus_tag="K0W65_001207"
     CDS             complement(976..1380)
                     /gene="stpA"
                     /locus_tag="K0W65_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBI3094548.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2048..2497
                     /gene="alaE"
                     /locus_tag="K0W65_001208"
     CDS             2048..2497
                     /gene="alaE"
                     /locus_tag="K0W65_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBI3094549.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2534..2878)
                     /locus_tag="K0W65_001209"
     CDS             complement(2534..2878)
                     /locus_tag="K0W65_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBI3094550.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3030..3260
                     /locus_tag="K0W65_001210"
     CDS             3030..3260
                     /locus_tag="K0W65_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094551.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3607..3852
                     /gene="nrdH"
                     /locus_tag="K0W65_001211"
     CDS             3607..3852
                     /gene="nrdH"
                     /locus_tag="K0W65_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBI3094552.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3849..4259
                     /gene="nrdI"
                     /locus_tag="K0W65_001212"
     CDS             3849..4259
                     /gene="nrdI"
                     /locus_tag="K0W65_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HBI3094553.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4232..6376
                     /gene="nrdE"
                     /locus_tag="K0W65_001213"
     CDS             4232..6376
                     /gene="nrdE"
                     /locus_tag="K0W65_001213"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBI3094554.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6386..7345
                     /gene="nrdF"
                     /locus_tag="K0W65_001214"
     CDS             6386..7345
                     /gene="nrdF"
                     /locus_tag="K0W65_001214"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBI3094555.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7700..8902
                     /gene="proV"
                     /locus_tag="K0W65_001215"
     CDS             7700..8902
                     /gene="proV"
                     /locus_tag="K0W65_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBI3094556.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8895..9959
                     /gene="proW"
                     /locus_tag="K0W65_001216"
     CDS             8895..9959
                     /gene="proW"
                     /locus_tag="K0W65_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBI3094557.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10017..11009
                     /gene="proX"
                     /locus_tag="K0W65_001217"
     CDS             10017..11009
                     /gene="proX"
                     /locus_tag="K0W65_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBI3094558.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11201..12380
                     /locus_tag="K0W65_001218"
                     /pseudo
     CDS             11201..12380
                     /locus_tag="K0W65_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12504..13241
                     /gene="ygaZ"
                     /locus_tag="K0W65_001219"
     CDS             12504..13241
                     /gene="ygaZ"
                     /locus_tag="K0W65_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HBI3094559.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13231..13566
                     /gene="ygaH"
                     /locus_tag="K0W65_001220"
     CDS             13231..13566
                     /gene="ygaH"
                     /locus_tag="K0W65_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBI3094560.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13657..14187
                     /gene="emrR"
                     /locus_tag="K0W65_001221"
     CDS             13657..14187
                     /gene="emrR"
                     /locus_tag="K0W65_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBI3094561.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14314..15486
                     /gene="emrA"
                     /locus_tag="K0W65_001222"
                     /pseudo
     CDS             14314..15486
                     /gene="emrA"
                     /locus_tag="K0W65_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15503..17041
                     /gene="emrB"
                     /locus_tag="K0W65_001223"
     CDS             15503..17041
                     /gene="emrB"
                     /locus_tag="K0W65_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBI3094562.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="K0W65_001224"
     CDS             complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="K0W65_001224"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBI3094563.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="K0W65_001225"
     CDS             complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="K0W65_001225"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBI3094564.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19399..19827)
                     /locus_tag="K0W65_001226"
     CDS             complement(19399..19827)
                     /locus_tag="K0W65_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI3094565.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="K0W65_001227"
     CDS             complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="K0W65_001227"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBI3094566.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(20538..20614)
                     /locus_tag="K0W65_001228"
     tRNA            complement(20538..20614)
                     /locus_tag="K0W65_001228"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20578..20580),aa:Arg,seq:acg)
     gene            complement(20679..20755)
                     /locus_tag="K0W65_001229"
     tRNA            complement(20679..20755)
                     /locus_tag="K0W65_001229"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20719..20721),aa:Arg,seq:acg)
     gene            complement(20820..20896)
                     /locus_tag="K0W65_001230"
     tRNA            complement(20820..20896)
                     /locus_tag="K0W65_001230"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20860..20862),aa:Arg,seq:acg)
     gene            complement(20961..21037)
                     /locus_tag="K0W65_001231"
     tRNA            complement(20961..21037)
                     /locus_tag="K0W65_001231"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21001..21003),aa:Arg,seq:acg)
     gene            complement(21041..21133)
                     /locus_tag="K0W65_001232"
     tRNA            complement(21041..21133)
                     /locus_tag="K0W65_001232"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21097..21099),aa:Ser,seq:gct)
     gene            complement(21449..21634)
                     /gene="csrA"
                     /locus_tag="K0W65_001233"
     CDS             complement(21449..21634)
                     /gene="csrA"
                     /locus_tag="K0W65_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBI3094567.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(21869..24499)
                     /gene="alaS"
                     /locus_tag="K0W65_001234"
     CDS             complement(21869..24499)
                     /gene="alaS"
                     /locus_tag="K0W65_001234"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBI3094568.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(24627..25127)
                     /gene="recX"
                     /locus_tag="K0W65_001235"
     CDS             complement(24627..25127)
                     /gene="recX"
                     /locus_tag="K0W65_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBI3094569.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(25370..26431)
                     /gene="recA"
                     /locus_tag="K0W65_001236"
     CDS             complement(25370..26431)
                     /gene="recA"
                     /locus_tag="K0W65_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBI3094570.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(26511..27008)
                     /gene="pncC"
                     /locus_tag="K0W65_001237"
     CDS             complement(26511..27008)
                     /gene="pncC"
                     /locus_tag="K0W65_001237"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBI3094571.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(27153..28238)
                     /gene="mltB"
                     /locus_tag="K0W65_001238"
     CDS             complement(27153..28238)
                     /gene="mltB"
                     /locus_tag="K0W65_001238"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBI3094572.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            28494..29056
                     /gene="srlA"
                     /locus_tag="K0W65_001239"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             28494..29056
                     /gene="srlA"
                     /locus_tag="K0W65_001239"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            29053..30012
                     /gene="srlE"
                     /locus_tag="K0W65_001240"
                     /gene_synonym="gutE"
     CDS             29053..30012
                     /gene="srlE"
                     /locus_tag="K0W65_001240"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBI3094573.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            30023..30394
                     /gene="srlB"
                     /locus_tag="K0W65_001241"
                     /gene_synonym="gutB"
     CDS             30023..30394
                     /gene="srlB"
                     /locus_tag="K0W65_001241"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBI3094574.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            30398..31177
                     /gene="srlD"
                     /locus_tag="K0W65_001242"
     CDS             30398..31177
                     /gene="srlD"
                     /locus_tag="K0W65_001242"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBI3094575.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            31282..31641
                     /gene="gutM"
                     /locus_tag="K0W65_001243"
     CDS             31282..31641
                     /gene="gutM"
                     /locus_tag="K0W65_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBI3094576.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            31708..32481
                     /gene="srlR"
                     /locus_tag="K0W65_001244"
                     /gene_synonym="gutR"
     CDS             31708..32481
                     /gene="srlR"
                     /locus_tag="K0W65_001244"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBI3094577.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            32474..33439
                     /gene="gutQ"
                     /locus_tag="K0W65_001245"
     CDS             32474..33439
                     /gene="gutQ"
                     /locus_tag="K0W65_001245"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBI3094578.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(33436..34950)
                     /gene="norR"
                     /locus_tag="K0W65_001246"
     CDS             complement(33436..34950)
                     /gene="norR"
                     /locus_tag="K0W65_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBI3094579.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            35137..36576
                     /gene="norV"
                     /locus_tag="K0W65_001247"
     CDS             35137..36576
                     /gene="norV"
                     /locus_tag="K0W65_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBI3094580.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            36573..37706
                     /gene="norW"
                     /locus_tag="K0W65_001248"
     CDS             36573..37706
                     /gene="norW"
                     /locus_tag="K0W65_001248"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBI3094581.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(37834..40086)
                     /gene="hypF"
                     /locus_tag="K0W65_001249"
     CDS             complement(37834..40086)
                     /gene="hypF"
                     /locus_tag="K0W65_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBI3094582.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(40239..40766)
                     /gene="hydN"
                     /locus_tag="K0W65_001250"
     CDS             complement(40239..40766)
                     /gene="hydN"
                     /locus_tag="K0W65_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBI3094583.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(40915..41925)
                     /gene="ascG"
                     /locus_tag="K0W65_001251"
     CDS             complement(40915..41925)
                     /gene="ascG"
                     /locus_tag="K0W65_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HBI3094584.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(41939..42058)
                     /locus_tag="K0W65_001252"
     CDS             complement(41939..42058)
                     /locus_tag="K0W65_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HBI3094585.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(42068..43777)
                     /gene="hycE"
                     /locus_tag="K0W65_001253"
     CDS             complement(42068..43777)
                     /gene="hycE"
                     /locus_tag="K0W65_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HBI3094586.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(43795..44718)
                     /gene="hycD"
                     /locus_tag="K0W65_001254"
     CDS             complement(43795..44718)
                     /gene="hycD"
                     /locus_tag="K0W65_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBI3094587.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(44721..46550)
                     /gene="hycC"
                     /locus_tag="K0W65_001255"
     CDS             complement(44721..46550)
                     /gene="hycC"
                     /locus_tag="K0W65_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBI3094588.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHS
                     LFKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGE
                     WVIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAE
                     NATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLL
                     IACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLK
                     LRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(46587..47198)
                     /gene="hycB"
                     /locus_tag="K0W65_001256"
     CDS             complement(46587..47198)
                     /gene="hycB"
                     /locus_tag="K0W65_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HBI3094589.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(47323..47784)
                     /gene="hycA"
                     /locus_tag="K0W65_001257"
     CDS             complement(47323..47784)
                     /gene="hycA"
                     /locus_tag="K0W65_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBI3094590.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            47996..48346
                     /gene="hypA"
                     /locus_tag="K0W65_001258"
     CDS             47996..48346
                     /gene="hypA"
                     /locus_tag="K0W65_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBI3094591.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            48350..49222
                     /gene="hypB"
                     /locus_tag="K0W65_001259"
     CDS             48350..49222
                     /gene="hypB"
                     /locus_tag="K0W65_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBI3094592.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            49213..49485
                     /gene="hypC"
                     /locus_tag="K0W65_001260"
     CDS             49213..49485
                     /gene="hypC"
                     /locus_tag="K0W65_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBI3094593.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            49485..50606
                     /gene="hypD"
                     /locus_tag="K0W65_001261"
     CDS             49485..50606
                     /gene="hypD"
                     /locus_tag="K0W65_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBI3094594.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            50603..51613
                     /gene="hypE"
                     /locus_tag="K0W65_001262"
     CDS             50603..51613
                     /gene="hypE"
                     /locus_tag="K0W65_001262"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBI3094595.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            51687..53765
                     /gene="flhA"
                     /locus_tag="K0W65_001263"
     CDS             51687..53765
                     /gene="flhA"
                     /locus_tag="K0W65_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HBI3094596.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(53802..54155)
                     /locus_tag="K0W65_001264"
     CDS             complement(53802..54155)
                     /locus_tag="K0W65_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBI3094597.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT        12893 a        14629 c        13882 g        12795 t
ORIGIN      
        1 agcttacgct tttactctca tttacgctgc tgaggctggt atatgttgca tttttagttt
       61 agagtttact taatttagaa aatacttaaa taaatatgac tgaactcgcg caattacagg
      121 ccagtaccga acaggcagcg gccttattga aagcaatgag ccaccctaaa cggttgctga
      181 ttctgtgcat gcttagcggt tcccccggca ccagcgcggg agagctgacg cgcattaccg
      241 gactgagcgc ctctgcgaca tcacagcatc tcgctcgtat gcgggacgaa gggcttatca
      301 acagccaacg ggatgcccaa cgcattctat attccattaa aaatgaggcg gtaaatgcca
      361 ttatcgccac cctgaaaaat gtctattgtc cgtaaggagt cactatggct ttgacaacca
      421 tttcgccgca tgatgcacaa gaattaatcg cacgcggcgc aaagttaatc gatattcgtg
      481 acgctgatga atatcttcgt gaacatattc ctgaagcaga tctggctcca ttatccgtgc
      541 tggaacagtc aggtcttcca cctaaattac gtcgcgagca aattatattc cactgccagg
      601 caggtaaacg caccagtaat aacgccgata aattagcagc gattgccgcc cccgcagaaa
      661 tatttttact cgaagatggg attgatggct ggaaaagagc tggattgcca gtagcggtaa
      721 ataaatctca acccttgccg ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat
      781 taaacggcgt tgtactgggt tataccgtaa atagcggttt cttcttatta agtggctttg
      841 ttggtgccgg gttactgttt gcaggaatca gcggtttttg tggaatggca aggttgttag
      901 ataagatgcc gtggaaccaa cgagcttgag aagcgacgcc ggacgcgccc tagcagcgac
      961 atccggcctc agtaattaga tcaggaaatc gtcgagagat ttaccttctg ccagcgcctg
     1021 agcaattggc ttcggtgtac ggccctgacc ggtccaggtt ttagtttcac cgttaacatc
     1081 agtgaattta tatttcgccg gacgcggctg gcgtttttta ccagcacgtg gtgcagcagc
     1141 agagctatta cccaataact cttccgggtt aattccgtca gctttcatca gctccagcca
     1201 ggtgctaatt ttttcctgac gctcagccag ttcacgctgc tgctgttctt cttcttcacg
     1261 tctttcttta gtgacaaccc tgaatttttc gagcatttct tcaagaacgt caatggagaa
     1321 ttcgcgagcc atcgcacgga gggtgcgaat gttatttaaa ctttgtaaca ttacggacat
     1381 aataaagaaa accttttaac gccaaaacaa aaaagtattt cgtcgcgtag tgtaagcgat
     1441 cacttattat atttccacaa aatttaatct atttacagtc ctgcgcgaga ttatttcagc
     1501 tgttattttc tgaaactctc agaataattt caagattata tattagccat tttgatgagt
     1561 aatttaggtt attggtgaac agagtggcgc tctctgctcg ctaactccgc aagcctgaca
     1621 aaaccgaagt gaataaaaat acaatataac agaataaaag aaaacacccc tcactggtgc
     1681 tttctgctgt taatgtacat tttctggcat caaaaatgct gtaaatgacc cggtcatgac
     1741 aaacaccgaa ttctctacgc ttatagcgtt acctcccccc caaactttca ttagtgaaat
     1801 taaaggcaag atcatatcta attgatttaa ttgaaaataa atcatattgg catgtaaaaa
     1861 tccacaatgt acaaaaaacg agccgttacg gaatatttta tctacaaaaa ctaactaaat
     1921 aaaaattttt cactaattga ttagtcatag gcagcgatat acgctatgca aaaatgcaga
     1981 tggcaatgag atccactgct ttcatctcca ttaacatccc attacgcttt tattaaggag
     2041 cattagcatg ttctcaccgc agtcacgctt gcgtcatgca gttgcagata cgttcgcgat
     2101 ggttgtttac tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg gaatgagctt
     2161 cgaacagtct ttttattcca gattggtagc gattccggtg aacatcttaa ttgcatggcc
     2221 atacggtatg taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg
     2281 gataaaaaat ctggctgata tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc
     2341 gatcttgtta gtggtgggcg cagactggca tcagattatg gcggcggtca gttcaaacat
     2401 cgttgtttcg atgttgatgg gggcggttta tggctacttc ctcgattatt gccgccgact
     2461 gtttaaagtc agccgttacc agcaggtaaa agcctgactc ttcttttcgc gactggcatc
     2521 gccagtcgcg tctttaactg gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa
     2581 cacgcgaact aaagccatgc gggataccgt aatgctcgtg tcgctcgcct gctaaataga
     2641 gcggaaattg ctggtaatga tcgcaagttt taatatcgga agcgggttca gccagtgttg
     2701 aactgcgttc ttttaatgtt ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc
     2761 gattaacata gacataattt tccccacggc ggtaaccata cgctttctgg gttaccacat
     2821 cgacagtaaa accgactttt tcaagtacgc gcgccacctc gtctggtcgt aaatacatat
     2881 ttgatcctcg ttattttcaa ccgcgggcct accttacctg attgcgcatt cacaacgctt
     2941 tcagaaaagt ccataaagcc gcgatgaacc tcagttaaga aaattatggt ctacactgaa
     3001 aattacatcg aattctaatg ggggatcata tgtttaaccg accgaaccgc aacgacgttg
     3061 atgatggcgt gcaggatatc cagaatgatg tcaatcaatt agctgacagc ctcgaatctg
     3121 tattgaagtc ctggggcagc gacgccaaag gggaagctga agccgcacgc agtaaagctc
     3181 aggcgttgct gaaagaaacc agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg
     3241 cgcgcgatgc cgttggctga gcggattctt ttgttcgtga aagaccctgg tgtagcgtgg
     3301 gtacagcagc tgcggtaggt atttttatcg gcgcactgtt aagcatgcgc aaatcgtagt
     3361 gcaaaaatga taataaatac gcgtctttga ccccgaagcc tgtcttcggg gtttcttttt
     3421 gcctggtgaa tcacaaaaat ccccctcccc cgtcacgctc atatccaggg taatttcgat
     3481 cactatttgc tatatattgt gtagttgaat cttttttcaa ctacatctag tatctctgta
     3541 tcaacagaga gacaacccga cgcgtatcat cgcgccgtat cttcatttta aacggaaata
     3601 cgaatcatgc gcataactat ttatactcgt aacgattgcg ttcagtgcca cgccaccaaa
     3661 cgggcgatgg aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa
     3721 gcagcggaag cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat tgctggcgat
     3781 cttagctggt ctggtttccg tccggacatg attaaccgtc tgcatccagc gccacacgcg
     3841 gccagtgcat gagccagctc gtctacttct ccagcagctc cgaaaacacg cagcgtttta
     3901 tcgaacgttt aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg
     3961 tagacgagcc ttacatcctg atcgtgccct cttacggcgg cggcggtacg gctggcgcgg
     4021 tgccacgaca ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg
     4081 ttattgcttc tggtaatcgc aactttggtg aggcgtatgg ccgcgccgga gatgtgattg
     4141 cccggaaatg cggcgtgccg tggctgtacc gttttgaact catgggtacg caaagcgata
     4201 tcgaaaacgt tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag aatgcctgac
     4261 gcaggaaacg atggattacc acgcgctgaa tgcgatgctt aatctttacg atagcgcagg
     4321 tcgcattcag ttcgataaag accgccaggc cgttgacgcc tttattgcga cgcatgtgcg
     4381 tccgaacagt gtgaccttca gtagccagca gcagcgcctg aactggctgg tcaacgaagg
     4441 ttactatgat gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc
     4501 ccacgcacac accagcggtt ttcgtttcca gacattcctc ggggcatgga agttttacac
     4561 cagctatacg ttgaagacat tcgacggtaa acgttatctg gaagattttg ccgatcgagt
     4621 cacgatggtg gcgctgacgc tggcacaagg cgatgagacg ctggcgttgc aactgaccga
     4681 tgaaatgctg tcaggacgct ttcagccagc cacgccaaca ttcctcaact gcggtaagca
     4741 gcagcgcggc gaactggttt cctgtttttt gctgcgtatt gaagacaata tggagtcgat
     4801 tggtcgggcg gtaaattccg cactgcagct gtcgaaacgc ggcggcggcg tagcattttt
     4861 gctgtcgaat ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg
     4921 cgtaattccg gtgatgaaaa tgttggaaga cgcattttcc tatgccaacc aactcggcgc
     4981 gcgtcagggg gcaggtgcag tctatttaca tgctcatcat cccgatattc tgcgttttct
     5041 cgacacgaaa cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt cgctgggcgt
     5101 ggtgatcccg gatatcactt tccatctggc aaaagagaat gcgcagatgg cgctgttttc
     5161 gccctatgac gttgagcgag tttatggcaa gccgtttgcc gatatcgcca ttagcgaaca
     5221 ctatgacgaa ctggttgccg atgaacgcat ccgcaaaaaa tacctcaacg cccgtgattt
     5281 cttccagcga ctggcagaaa tccagtttga gtccggctat ccctacatca tgtatgaaga
     5341 cacagtaaac cgtgctaacc ctatcgccgg gcgcataaat atgagtaatc tctgctcaga
     5401 aattttgcag gttaacagcg cctcagagta tgacgagaat ctcgactatg cccgcacagg
     5461 ccatgatatt tcctgcaatt taggttcgtt gaatattgcg cacaccatgg attcccccga
     5521 tttttcccgc acggtagaga ctgccgtgcg cggtttaacg gcggtgtcag atatgagtca
     5581 tatccgcagc gtgccgtcca tcgaagccgg aaatgccgcc tcgcacgcca tcggactggg
     5641 gcagatgaat ttacatggtt atctggcgag ggaaggcatc gcttatggtt cgccggaagc
     5701 cctggatttc accaatctct atttctatac catcacctgg cacgcactgc gtacctcgat
     5761 gttgctggcg cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac gctatgccag
     5821 tggtgaatat ttcagccaat atctgcaagg taactggcag ccgaaaacgg cgaaagttgg
     5881 cgaactgttt gcccgcagct gtattacgtt acctacccgt gagatgtggg cgcaactgcg
     5941 cgacgacgtg atgcgctacg gcatatacaa ccagaatctc caggcggtgc cgccaaccgg
     6001 gtctatctct tatatcaacc atgctacgtc gagtattcat ccgattgtgg cgaaagtaga
     6061 gatacgcaaa gagggcaaaa caggacgcgt ttactaccct gccccgttta tgactaacga
     6121 aaatctggcg ctgtatcagg acgcttacga aattggcgca gaaaagatca tcgacaccta
     6181 cgcggaagcg actcgccatg tcgatcaggg gctgtcgctg acgctgtttt tccccgatac
     6241 cgccaccact cgcgatatca acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac
     6301 gctctattac atccgcctgc gtcagatggc gctggaaggc actgaaattg aaggctgcgt
     6361 ctcctgtgca ctttaaggaa tatctatgaa actctcacgt atcagcgcca tcaactggaa
     6421 caagatatct gacgataaag atctggaggt gtggaatcgc ctgaccagca atttctggct
     6481 accagaaaag gtgccgctgt cgaacgatat tcccgcctgg cagacattaa ctgtcgtaga
     6541 acaacaactg acgatgcgcg tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt
     6601 tatcggcgcg ccttcgctga tgcccgatgc gctcacgcct catgaagagg cggtattatc
     6661 gaatatcagc tttatggaag cggttcatgc ccgctcttac agttcgattt tctcgacgct
     6721 gtgccagacc aaagatgtcg atgccgccta cgcctggagt gaagaaaacg caccgttgca
     6781 gcgaaaagct cagattattc agcaacatta tcgcggtgat gatccgctga aaaagaaaat
     6841 cgccagtgtg tttcttgaat cttttttgtt ctattccggt ttctggctgc cgatgtattt
     6901 ttccagccgc ggaaagctga ccaataccgc ggacctgatc cgtctgatta tccgcgatga
     6961 agcagtccac ggttactaca taggctataa atatcagaaa aacatggaaa agatatctct
     7021 gggacaacgt gaagagttga agagtttcgc cttcgatttg ttgctggaac tctacgacaa
     7081 cgagttgcaa tacaccgatg agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc
     7141 gtttctctgt tacaacacca ataaggcttt gatgaatctg ggctacgaac cgttatttcc
     7201 cgcagaaatg gcggaagtga atccggcaat cctcgccgcg ctttcgccga atgccgatga
     7261 aaatcacgat ttcttttccg gttcaggctc ctcttatgtg atggggaaag cggttgaaac
     7321 agaagatgaa gactggaatt tctgagggtg ttattttcaa aaatatcact acccgcagca
     7381 gggaaataat tcccgccaaa tagcttttta tcacgcaaat attctgtagt gatctacact
     7441 gatactctgt tgcattattc gcctgaaact acaatattca ggcgtttttt cgctatctat
     7501 gacaaaaaat atcaactttt ctcgatttgc tctcagccct tatatcacgg aaaattccgg
     7561 cgatttgctc gcatcaatat tcatgccaca tttgccctca ggggttgcct cagattctca
     7621 gtatgttagg gtagaaaaaa gtgactattc ccattgggta atatatcgac ataaacaaat
     7681 aaagaaatca ttctattgca tggccattaa attaaaaatt aaaaatcttt ataaaatatt
     7741 tggtgagcat ccacagcgag cgttcaaata tatcgaacaa ggactttcaa aagaacaaat
     7801 tctggaaaaa actgggctat cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg
     7861 cgagatattt gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg tacgccttct
     7921 caatcgcctg attgaaccca cccgcgggca agtgctgatt gatggtgtgg atattgccaa
     7981 aatatccgac gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc
     8041 ctttgcctta atgccgcata tgaccgtgct ggacaatact gcgttcggta tggaattggc
     8101 cggaattaat gccgaagaac gccgggaaaa agcccttgat gcactgcgtc aggtcgggct
     8161 ggaaaattat gcccacagct acccggatga actctctggc gggatgcgtc aacgtgtggg
     8221 attagcccgc gcgttagcga ttaatccgga tatattatta atggacgaag ccttctcggc
     8281 gctcgatcca ttaattcgca ccgagatgca ggatgagctg gtaaaattac aggcgaaaca
     8341 tcagcgcacc attgtcttta tttcccacga tcttgatgaa gccatgcgta ttggcgaccg
     8401 aattgccatt atgcaaaatg gtgaagtggt acaggtcggc acaccggatg aaattctcaa
     8461 taatccggcg aatgattatg tccgtacctt cttccgtggc gttgatatta gtcaggtgtt
     8521 cagtgcgaaa gatattgccc gccggacacc gaatggctta attcgtaaaa ctcctggctt
     8581 cggcccacgt tcggcactga aattattgca ggatgaagat cgcgaatatg gctacgttat
     8641 cgaacgcggt aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac
     8701 gcagcagcaa ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac
     8761 gcctcttagc gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga
     8821 cgaggaccaa cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg
     8881 tgagggggta aataatggct gatcaaaata atccgtggga taccacgcca gcggcggaca
     8941 gtgccgcgca atccgcagac gcctggggta caccgacgac tgcaccggct gacggcggtg
     9001 gtgctgactg gctgaccagt acgcctgcgc caaacgtcga gcattttaat attctcgatc
     9061 cgttccataa aacgctgatc ccgctcgaca gttgggtcac tgaagggatc gactgggttg
     9121 ttacccattt ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat atcctcaacg
     9181 gtttccagca attgctgctg ggtatgccag cgccagtggc gattatcgtt ttcgctctca
     9241 tcacctggca gatttccggg gtcggaatgg gcgtggcgac gctggtctcg ctgattgcca
     9301 tcggcgcaat cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg ttaactgccc
     9361 tgctgttctg tatcgtcatc ggtttgccgt tggggatatg gctggcgaga agtccgcgag
     9421 cggcgaaaat tattcgtcca ctgcttgatg ccatgcagac cacgccagcg tttgtttatc
     9481 tggtgccaat cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca
     9541 tctttgcgct gccgccgatt atccgtctga ccattctggg gattaaccag gttccggcgg
     9601 atctgattga agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc
     9661 agttaccgct ggcgatgccg accattatgg cgggcgttaa ccagacgctg atgctggccc
     9721 tttctatggt ggtcattgcc tcgatgattg ccgtcggcgg gctgggtcag atggtacttc
     9781 gcggtatcgg tcgtctggat atggggcttg ccaccgttgg cggcgtcggg attgtgatcc
     9841 tcgccattat cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca
     9901 accgtcgctg gtacaccact ggccctgttg gtctgctgac ccgcccattc attaagtaac
     9961 tctgcacttg cccggtgacg ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc
    10021 gacatagcgt actttttgcg acagcgtttg ccacgcttat ctctacacaa acttttgctg
    10081 ccgatctgcc gggcaaaggc attactgtta atccagttca gagcaccatc actgaagaaa
    10141 ccttccagac gctgctggtc agtcgtgcgc tggagaaatt aggttatacc gtcaacaaac
    10201 ccagcgaagt ggattacaac gttggctaca cctcgcttgc ttccggcgat gcaaccttca
    10261 ccgccgtgaa ctggacgcca ctccatgaca acatgtacga agctgccggt ggcgataaga
    10321 aattttatcg tgaaggggta tttgttaacg gcgcagcaca gggttacctg atcgataaga
    10381 aaaccgccga ccaatacaaa atcaccaaca tcgcacaact gaaagatccg aagatcgcca
    10441 aactgttcga taccaacggc gacggtaaag cggatttaac cggttgtaac cctggctggg
    10501 gctgcgaagg tgcgatcaac caccagcttg ccgcgtatga actgaccaac accgtgacgc
    10561 ataatcaggg gaactacgca gcgatgatgg ccgacaccat cagtcgctac aaagagggca
    10621 aaccggtgtt ttattacacc tggacgccgt actgggtgag taatgagctg aagccaggga
    10681 aagatgtggt ctggttgcag gtgccgttct ccgcactgcc gggcgataaa aatgccgata
    10741 ccaaactgcc gaatggcgcg aattacggct tcccggtcag caccatgcat atcgttgcca
    10801 acaaagcctg gaccgagaaa aatccggcgg cagccaaact gtttgccatt atgcagttgc
    10861 cagtggccga tattaacgcc cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg
    10921 atattcaggg ccacgttgat ggctggatca aagcccacca gcagcagttc gatggctggg
    10981 tgaatgaggc gctggcagcg cagaagtaat ttttattcgg gcggataagg cgttttcgcc
    11041 tcatccgccg ctctgtacac aatgcctgat gcgacgctga cgcgtcctat catgcctaca
    11101 aaccacatcg cacatttcaa caagctatct ttcatcccat attcatcaac atccgctatt
    11161 attgatttcc agcttaatca tcacctgatg aacaaaaata atgactaaac ctaatcatga
    11221 gcttagcccg gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg tagccagtaa
    11281 ctattacgcc cagccattgc tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc
    11341 ggcaggcttt attgttaccg ccgcgcagtt gggctatgcc gcaggtctac tgtttcttgt
    11401 tcccctcggt gatatgtttg aacgccgccg cctgattgtc tcgatgacct tactggcagc
    11461 gggcggtatg ttaatcactg ccagcagtca gtcactggcg atgatgatcc tcggtacggc
    11521 attaaccggt ttattctcag tcgtggcaca aattctggtt ccgctggcag cgacgctggc
    11581 ttcaccggat aaacgcggca aagtggttgg caccattatg agcggtctgc tgttggggat
    11641 attgctggca cgaaccgttg ccgggttact ggcaaacctc ggcggctggc gcaccgtctt
    11701 ttgggttgct tcggtgttaa tggcactgat ggcgctggca ttatggcgtg gtctgccaca
    11761 aatgaaatca gaaacccacc tcaactaccc acagttgttg ggttccgttt tcagcatgtt
    11821 catcagcgat aagatcctgc gcacccgcgc gttgctgggc tgcatgacct ttgccaactt
    11881 cagcattctc tggacctcaa tggccttttt gcttgccgct ccacctttta actacagcga
    11941 tggtgacatt ggtctgtttg ggcttgcggg agctgccgga gcgttgggcg ctcgtccggc
    12001 gggcggtttt gccgacaagg gcaaatcgca ccacaacttt cggtctgctt ctgctgttac
    12061 tttcatggct ggcaatctgg tttggtcaca cttccgtact ggcgttgatt atcggcattc
    12121 tggtgctgga cctcaccgta cagggcgtgc atatcactaa ccagacggta atttatcgaa
    12181 tacatcctga tgcgcgtaat cgcctgaccg caggttacat gaccagctac tttattggcg
    12241 gtgccgccgg ttcgctaatt tcagcctcag cctggcaaca tggcggttgg gctggcgttt
    12301 gtctggctgg cgcgacgatt gccctggtta acttactggt ctggtggcga ggttttcatc
    12361 gtcaggaagc cgcaaattaa gcaatcagca accgttttcg tggctttaca cttataaggg
    12421 tgttaagaag cccatcagtc tgataaggtt aagatattca ttcagtctat ttataatatt
    12481 aacaatcgtt aagcgtacac tctatggaaa gccctactcc acagcctgct cctggttcgg
    12541 cgaccttcat ggaaggatgc aaagacagtt taccgattgt tattagttat attccggtgg
    12601 cctttgcgtt cggtctgaat gcgacccgtc tgggattctc tcctctcgaa agcgtttttt
    12661 tctcctgcat catttatgca ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg
    12721 ggagtagttt gtgggttgct gcactgagcg tcatggcaat ggatgttcgc catgttttgt
    12781 atggcccgtc actgcgtagc cgtattattc agcgtctgca aaaatcgaaa accgccctat
    12841 gggcgtttgg cctgacggat gaggtttttg ccgccgccac cgcaaagctg gtacgcaata
    12901 atcgccgctg gagcgagaac tggatgatcg gcattgcctt cagttcatgg tcatcgtggg
    12961 tatttggtac ggtaataggg gcattctccg gcagcggctt gctgcaaggt tatcccgccg
    13021 ttgaagctgc attaggtttt atgcttccgg cactctttat gagtttcctg ctcgcctctt
    13081 tccagcgcaa acaatctctt tgcgttaccg cagctttagt tggtgccctt gcaggcgtaa
    13141 cgctattttc tattcccgtc gccattctgg caggcattgt ctgtggctgc ctcactgcgt
    13201 taatccaggc attctggcaa ggagcgcccg atgagctatg aggttctgct gcttgggtta
    13261 ctggttggcg cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat
    13321 gcccgcccaa ccaaacgtgg cgcggtaggt attttgctcg acaccattgg catcgcctcg
    13381 atatgcgctc tgctggttgt ctctaccgca ccagaagtga tgcacgatac acgccgtttc
    13441 gtgcccacgc tggtcggctt cgcggtactg ggtgccagtt tctataaaac acgcagcatt
    13501 atcatcccaa cactgcttag tgcgctggcc tatgggctcg cctggaaagt gatggcgatt
    13561 atataaccca caagaatcat ttttctaaaa caatacattt actttatttg tcactgtcgt
    13621 tactatatcg gctgaaatta atgaggtcat acccaaatgg atagttcgtt tacgcccatt
    13681 gaacaaatgc taaaatttcg cgccagccgc cacgaagatt ttccttatca ggagatcctt
    13741 ctgactcgtc tttgcatgca catgcaaagc aagctgctgg agaaccgcaa taaaatgctg
    13801 aaggctcagg ggattaacga gacgttgttt atggcgttga ttacgctgga gtctcaggaa
    13861 aaccacagta ttcagccttc tgaattaagt tgtgctcttg gatcatcccg taccaacgcg
    13921 acgcgtattg ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtaa aagcgataac
    13981 gatcgccgct gcctgcatct gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt
    14041 ttaccaccgc agcataactg cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa
    14101 gatcagctcg agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac
    14161 ggtgtggttc tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc agatttataa
    14221 aagaaaaaat gaccggccag catcgcaaca tgctggcctt tttggcaagc aggtcggctc
    14281 agccgatgag ttaagaagat cgtggagaac aatatgagcg caaatgcgga gactcaaacc
    14341 ccgcagcaac cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg
    14401 ctctttataa ttattgccgt agcgataggg atttattggt ttttggtact gcgtcatttc
    14461 gaagaaaccg atgacgcata cgtggcaggg aatcagattc aaattatgtc tcaggtgtct
    14521 ggcagcgtga cgaaagtctg ggccgataac accgattttg taaaagaagg cgatgttctg
    14581 gtcactctcg acccgacaga tgctcgccag gcgtttgaaa aagccaaaac tgcactggct
    14641 tccagcgttc gccaaaccca ccagctgatg attaacagca aacagttgca ggcgaatatt
    14701 gaggtgcaaa aaatcgccct cgcgaaagca caaagcgact acaaccgccg tgtgccgctg
    14761 ggcaatgcca acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt
    14821 gcccaggcgc aactggacgt cgctattcaa caatacaatg ccaatcaggc gatgattctg
    14881 gggactaaac tggaagatca gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc
    14941 tagctggcgc tggagcgtac ccgtattgtc agtccgatga ccggttatgt ttcccgtcgt
    15001 gccgtgcaac cgggtgcgca aattagccca acgacgccgc tgatggcggt cgttccagcc
    15061 accaatatgt gggtggatgc caactttaaa gagacgcaga ttgccaatat gcgcatcggt
    15121 cagccggtca ccatcaccac cgatatttac ggcgatgatg tgaaatacac cggtaaagtg
    15181 gttggtctgg atatgggcac aggtagcgcg ttctcactgc ttccggcgca aaatgcgacc
    15241 ggtaactgga tcaaagtcgt tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag
    15301 ctggagcaat acccgctgcg cattggtttg tccacgctgg tgagcgtcaa taccactaac
    15361 cgtgacggtc aggtactggc aaataaagta cgttcaacac cggtcgcggt aagtaccgcg
    15421 cgtgaaatca gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct
    15481 ggctaatcca gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt
    15541 cattatgacg attgcgctgt cactggcgac attcatgcag gtgctgaact ccaccattgc
    15601 taacgtggcg atccccacta tcgccgggaa tctgggctca tcgctcagcc aaggaacgtg
    15661 ggtaatcact tctttcgggg tggcgaatgc catctcgatc ccgcttaccg gctggctggc
    15721 aaagcgcgtc ggggaagtga aactgttcct ttggtccacc atcgcctttg ctattgcgtc
    15781 ttgggcgtgt ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg
    15841 gattgtcgcc gggccgttga tcccgctttc gcaaagtcta ttgctgaata actacccgcc
    15901 agccaaacgc tcgatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg
    15961 cggcccgatc ctcggcggtt atatcagcga taattaccac tggggctgga tattcttcat
    16021 caacgtgccg attggcgtgg cggtggtgtt gatgacactg caaaccctgc gcggacgtga
    16081 aacccgcacc gaacggcggc ggattgatgc cgtggggctg gcactgctgg ttattggtat
    16141 cggcagcctg cagattatgc tcgaccgcgg taaagagctg gactgttttt catcacagga
    16201 aattatcatt cttaccgtgg tggcggtggt ggctatctgc ttcctgattg tctgggagct
    16261 gaccgacgat aacccgatag tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg
    16321 ctgcttgtgt atcagcctcg cgtatatgct ctacttcggc gctattgttc tgctgccgca
    16381 gttgttgcag gaggtctacg gttacacggc gacctgggca ggtttggcct ctgcgccggt
    16441 agggattatt ccggtgattc tgtcgccgat tatcggccgc ttcgcgcata aactggatat
    16501 gcggcggctg gtaaccttca gctttattat gtatgccgtc tgcttctact ggcgtgccta
    16561 tacctttgaa ccaggtatgg atttttgcgc gtcggcctgg ccgcagttta tccaggggtt
    16621 tgcggtggtc tgcttcttta tgccgctgac caccattacg ctgtctggtt tgccaccgga
    16681 acgactggcg gcggcatcga gcctctctaa ctttacgcga acgctggcgg ggtctatcgg
    16741 cacgtcgata accacgacca tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac
    16801 tgagtcggta aacccgttca acccgaatgc ccaggcgatg tacagtcaac tggaagggct
    16861 tgggatgacg caacagcagg catcaggctg gattgcccag cagatcacca atcaggggct
    16921 gattatttcc gccaatgaga tcttctggat gtcagccggg atattcctcg tactgctggg
    16981 gctggtgtgg tttgctaaac cgccatttgg cgcaggtggc ggcggaggcg gtgcgcacta
    17041 agtacaacta agccagttca tttgaactgg cttttttcaa ttaattgtga agatagttta
    17101 ctgactagat gtgcagttcc tgcaacttct ctttcggcag tgccagttct tcgttgctgt
    17161 tgatgcgtac ctcacgttcc agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt
    17221 gcatctggta agtgccacac tggtagacgt tcagttccgg gatctgattc tgatcctgca
    17281 ctttcagcac gtcttccatt gccgctttcc aggcatcagc aacacgctgc tcatctggcg
    17341 taccaatcag actcatataa aaaccggtgc ggcagcccat tggcgagata tcgataatct
    17401 ctacaccatt accgttaaga tggttacgca taaaaccagc aaacaggtgc tccagggtat
    17461 ggatccctct ttctggcatc acttctttgt tcggcacgca gaagcgcaga tcgaacacgg
    17521 tgattgcgtc gccatgcggg gtgttcattg ttttcgccac ccgaactgca ggcgcttcca
    17581 tccgggtatg atcgactgtg aagctatcta acaacggcat ttagccacct ccggtaattt
    17641 ttttaaaaat tttctgaact ctttcttccc aggcgagtct gagtatatga aagacgcgca
    17701 tttgttatca tcatccctga attcagagat gaaattttgg ccactcccga gtggcctttt
    17761 tcttttctgt caggcgtgtt tttccagcca caccgcaaac ggttcggtat cagcggtttc
    17821 catttcctgc tgacggcgtt cagatgcctc gcgctcggct acaaaatcct cttcgcgcag
    17881 aatttccagc ggctcttcac gcagcagatt acggtaggct tctgcaaacg ctttgcctgt
    17941 tccgccaata ccagtatcaa tcatagacct taagatacgg gcagagaaag tcagatcggg
    18001 attatcgaag caggcaacca gttcatcaca cactttctga tacgcttcgc cgccgttaat
    18061 gctatccagc gtttgcgcga cgcgtttcag atcgcggaac agatctttac ccacctgcag
    18121 taacgggaac tgcgcggttt cgcagccgat acccagcgtc aggcccggtt tgcgaccttc
    18181 aaggatcacc cggttccagt taacgcgcgt acaggcaagt tcgctactgc tcatttccgg
    18241 tgcatcagcc agcgcacacc agaccataaa caggtcgagg aatcgcacct gctgttcatc
    18301 tacaccaatc ggcgagaacg ggttgatgtc cagcgaacgc acttcaatat attcaatgcc
    18361 gccacgtaac agcgcatcag aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat
    18421 cggcgcatac agttcgtttt caatctgcag cacgttgcta ttgatttgca gcctcttacc
    18481 gtctttctca ataccaatct tcgcgtactc ttccgatggc gttttgattg cctgtttaag
    18541 gcccgctacg tactcgtaaa gatcgttgaa ggtaatacca agattgcttt gcgatttatt
    18601 ggtatagccg agatcgctca aacgaagaga ggtcgcatac ggcaggtaat acataccgca
    18661 ctcggttttc tcaaacggca gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc
    18721 cggagatgca ccaaacagat aaggaataac ccaaccgaaa cgatagtaat tgcggataac
    18781 gcggaaatag cccgcagaaa ttttctcttt ggcatcagcg cccgagatat caccgcactt
    18841 cgcttgccag aatgccattg gcaaagagaa attgtagtgc acgccggaaa tggtttgcat
    18901 cagcgcgccg tagcgatttt tcagcccttc acgatacagc gttttaaagc gtccggtgtt
    18961 agaagtgccg tactgtgcca gttcgatgtc ctgaccttct gcgatgtagc atggcatact
    19021 taacggccac atccgctcat cgcccatatt gcgcgccgta taacgatgca gatcgcgcat
    19081 aaaggtcagc atatgttcaa tatcaccatc cactggtgta atgaattcca gcaatgcttc
    19141 cgcaaaatcg gtagtaatcc atttgtgcgt cagtgcggaa cctaatgctt caggatgacc
    19201 tgttgttgcc agtgtgccat cagcattaac acgcaaagtt tcgcgctcca gcccacgctg
    19261 tatccccttt aacgcctgag gatgtttttc cagccaggcc agcgcctgtg atacgtccgg
    19321 gatcaaattg acctcccgcc tgtcaaaatc gttttaatta gcataactgt aatggtgacc
    19381 atatgtgcag gcctacaatt agtgccacca catcatgccc tgaacggtcg ctgctgcaac
    19441 tgcaacatag cgtaacgctt taccaaggca caaaaaaaag attactggcc cccaggagat
    19501 gcgcatccat cccgctaaca gacacagtaa atcgccaacc acgggcatcc agcttaataa
    19561 tagcgtgact gcaccatagc gtttcagcca gccggtagct ttctcttgcc agcgcgatgt
    19621 tttacgcaat ggaaagaaac gcccaaggat aacgttagtt aaccctccaa ggctattacc
    19681 cattgttgct gttaaaacta aaacccaggg atgactgatc ccggaaagca acattgccac
    19741 cagcacgact tcggagttgc cgggtaatag tgtagcgctg agaaaactac tggcaaacaa
    19801 cgagaaaagc gataacgctt cactcacagc aagcgaacat ccacagcgtc catgcctgct
    19861 gcacgggccg cctgaatacc gaaatcggca tcttcaaaga ccacacactg cgtcggttgc
    19921 acgcccatac gctgcgcgca caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg
    19981 tgatcagcag cgacgacggc gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc
    20041 tcagcgatgg cgctttcact ccccgttcct acagccattg ggcgacgacc atgccaactt
    20101 ttcaccactt caacaagagg aagcggctcg acactatcca gcagcatact tcttactgct
    20161 tctgtttttt cacgcgctaa cgcatgcggg tcgagatcgg cctgattcag ctcaataatt
    20221 gcctgagcaa tacgccaggt gggcgatcca ttaagcgcaa tcatcgcctg aatatcgtac
    20281 tgaagaccgt agtgccctaa tacttcgcgc cacgctttac ggtgcgtagg cgccgtatcc
    20341 aggattgtgc catccatatc aaaaattaaa cctgcataac gctcgtacat ggtcttctcg
    20401 caagtcgaaa aatatgacgc tactttagcg taattgcttg attttgtcgc tgatggcgaa
    20461 ggagaagaaa attcaggaaa tggataaagt agttatttag aaatgagata tttttgaagg
    20521 aaattttttg gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt
    20581 agccgagtac tctatccagc tgagctacgg atacatcggg aaacttactt tactgcagat
    20641 tttttgatac cgctactaaa gccgtatcaa gtaagagatg gtgcatccgg gaggattcga
    20701 acctccgacc gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcatcgg
    20761 gaaacttact ttactgcaga ttttttgata ccgctactaa agccatatca agtaagagat
    20821 ggtgcatccg ggaggattcg aacctccgac cgctcggttc gtagccgagt actctatcca
    20881 gctgagctac ggatgcatcg ggaaacttac tttactgcag attttttgat accgctacta
    20941 aagccgtatc aagtaagaga tggtgcatcc gggaggattc gaacctccga ccgctcggtt
    21001 cgtagccgag tactctatcc agctgagcta cggatgcaaa tggcggtgag gcggggattc
    21061 gaaccccgga tgcagctttt gaccgcatac tcccttagca ggggagcgcc ttcagcctct
    21121 cggccacctc accacacgcc tcttacgagt gcttcgaaga acttgtttat cgctcatcat
    21181 cgctgcgtgg cgcacatatt actttctgag acttataagt caaacaattt ttcccacact
    21241 tttatcgttt gcacacttca cgctcaatta gtctgtaaaa gcgtcaaaaa gagtgtttta
    21301 tctacagaaa aacggaggtc tggcagatag cagtaatgca aaaaaatgga gacttaagtt
    21361 gaatgaacgg gagtaaagcg aaaagactat agagtgaagg agaaattttg agggtgcgtc
    21421 tcaccgataa agatgagacg cggaaagatt agtaactgga ctgctgggat ttttcagcct
    21481 ggatacgctg gtagatctct tcacggtgaa cagaaacttc cttcggggca tttacgccaa
    21541 tacgtacctg gttgcccttt acccctaaaa ctgtcacggt gacctcatcc ccaatcatga
    21601 gggtctcacc aactcgacga gtcagaatca gcattctttg ctccttgaaa gattaaaaga
    21661 gtcgggtctc tctgtatccc ggcattatcc atcatataac gccaaaaagt aagcaatgac
    21721 aaacacatta catgtaagca gtcatggcat tacattctgt taaacctaag tttagccgat
    21781 atacacaact tcaacctgac tttatcgttg tcgatagcgt taatgcgaat gccgtgaagc
    21841 gagtccacgg cattgcctga cgcttatatt attgcaattt cgcgctgacc cagcctttca
    21901 cactggctaa cgctgcaggt aaggccgcag catccgtacc accggcttgc gccatgtcag
    21961 gacgtccacc acccttgccg cccacctgct gagcgaccat accaatcagt tcccctgctt
    22021 tcacacgatc tgtgacgtcc ttagatacgc ctgcaatcag agaaacctta ccttcggcta
    22081 ccgttgccag cacgataatt gtcgacccca gctgattttt taaatcgtca accatggtac
    22141 gcaacatttt cggctcaaca ccgctaagct cgctaaccaa cagcttaaca ccattaacat
    22201 caattgcctt actggaaaga tttgcgctct cctgtgcggc agcttgttct ttaagctgtt
    22261 gtagttcttt ttctaactga cgcgtacgtt ccagtactga gcgcacttta tccgccagat
    22321 tattgctatc gcctttcagc agatgcgcga cttcgcttaa gcgatcgctg tctgcatgaa
    22381 cggtggtgat agcgccttct ccggttaccg cttcgatacg acgaacgcct gcagcagtac
    22441 ccgattcaga gatgatgcgg aacagaccaa tatcaccagt gcggctggcg tgagtaccgc
    22501 cacacagctc ggtggagaaa tcgcccatgc tcagcacgcg tacacgctca tcatacttct
    22561 cgccgaacag cgccatcgca cctttcgctt tcgccgcttc gagattcatg atgttggttt
    22621 cgatcggcaa attgcgacga atctgtgcgt tcaccaggtc ttcgaccgca cgaatctctt
    22681 ccggtttcat cgcttcgttg tgtgagaagt cgaagcgcag caccttgtcg ttaaccagtg
    22741 aacctttctg cgatacatga gtccccagaa cctggcgcag cgcagcgtgc atcaggtgcg
    22801 ttgcggagtg attcagacga atacgggcgc gacgagcctc atcaatatca gcctgcacag
    22861 cgtcgcccac tttcagagaa cccgcagcaa gtttaccgat gtgaccaatc gcctggccgt
    22921 atttctgcgt atcttccacc gcgaaggaga agttagcgcc tttcagttcg cctttatcac
    22981 caacctgacc gccggattcc gcatagaatg gcgtttgatc cagcacgacc acagcttcct
    23041 ggccggcatt gatggcatca accgctttac catcaacaaa cagcgcggtc actttgccgt
    23101 tcagttccag atggtcatag cctttaaatt cagatgcact atcaacacgg atcattgcgt
    23161 tgtaatcggc accaaagccg ctggcttcgc gcgcgcgacg acgctgctct tccattgctg
    23221 cttcaaaacc agcttcgtca actttgatgt tgcgctcacg acaaacatca gccgtcaggt
    23281 caaccgggaa gccataggtg tcgtacagac ggaaagccgt ttcaccatcc agcgtatcac
    23341 cagaaagttt tgccagctct tcatccagca acgccagacc gcgctccaga gtacgggcaa
    23401 actgctcttc ttcagtcttc agcacctgct caacctgcgc ctgctggcgt ttcaggtctt
    23461 cacccgcaga gcccataacg tcgatcagcg gaccaaccag tttgtagaag aaggtttctt
    23521 tcgcgccgag catattgccg tgacgcactg cgcgacgaat gatacgacgc agtacataac
    23581 cacggttttc attggacggc attacgccat ccgcgatcag gaacgcacaa gaacgaatgt
    23641 ggtcagcgat tacgcgcagc gatttattgc tcagatcggt cgcgccagtg actttcgcta
    23701 ccgcctggat caacgtgcgg aacaggtcga tgtcatagtt agagttaacg tgttgcagca
    23761 ccgcagcaat acgctccaga cccataccgg tatctacaga cggcttcggc agcggttcca
    23821 tcgtgccatc tgcctggcgg ttgaactgca tgaagacgat gttccagatc tcaatgtagc
    23881 ggtcgccgtc ttcttccgga cttcccggag ggccacccca aatgtggtcg ccgtgatcgt
    23941 agaagatttc ggtgcacggg ccgcacggac cagtgtcacc catctgccag aagttgtcag
    24001 atgcgtatgg cgcacctttg ttatcgccga tgcgaataat acgttcgcgc gggatcccga
    24061 cttctttttc ccagatttcg taggcttcgt cgtcgctttc atagacggta acccacagac
    24121 gctctttcgg cagggcaaac catttttcgc tggtcagcag ttcccatgca aactgaatgg
    24181 catcgtgttt gaaatagtcg ccgaagctga agttgcccag catttcgaag aaggtatggt
    24241 gacgcgcggt gtaaccgacg ttttccaggt cgttgtgttt accacccgca cgcacgcagc
    24301 gttgggaagt ggtagcgcgg gaataattac gcttgtcgag cccaaggaac acatccttga
    24361 actggttcat cccggcgttg gtaaacaaca aagttgggtc gttatggggt accagggagc
    24421 tgctggcaac tacctgatgt cccttactat ggaaaaagtc gagaaacgcc tgacggatct
    24481 cagcggtgct cttgctcata attatcctga aatcaagcta acgaaatatc gccaccagct
    24541 ccagcgtgtc ttaaccgccg ggctggtaac tgaaaagtgg gaataagata agttttcttg
    24601 actgggaagt aaaataccgt atgcgttcag tcggcaaaat ttcgccaaat atcctggata
    24661 tcttccatca gatagccacg atagagcaga aaacgctgga tcttaacttt ttctgaaaag
    24721 acagttggca aaggttcacc atattttcgc gtcgcctgat cgcgcgccag tgcgcaccag
    24781 tcgatgtcac attcacgcat cgctttttct gtcgcttcgc gggaaatacc tttctgattc
    24841 agttcctgac gaatacgtgc aggtccatac cctttgcggc tacggctggc gataaagcgc
    24901 gcaacaaatc ggctgtcatc gagatagcca tgttcatggc accaggcaat aacgcgctcg
    24961 taatcttctg ccgtagcatc aatctcttct gggccatttt tgcccataat cggtgccgcg
    25021 agtttacgtc gcagttcttg ctcactgtga tcgcgcaccg ccagaatgcg taccgcacga
    25081 tccaacaggc gagcatatgc cgggcgacgg gatgttgatt ctgtcatggc atatccttac
    25141 aacttaaaaa agcaaaaggg ccgcagatgc gccccaggtt ttgctgataa acagatttaa
    25201 tagtcaatga gtatgcagaa tgttcccgca ggcccaattc gttagaggat cgaggtgaga
    25261 gtaatttttc acgccagtgc tggcctgttc ccctcaccct aaccctctcc ccaaaggggc
    25321 gaggggaccg tccgtgcaca cattatctat tatcatcaat aacgaacgat taaaaatctt
    25381 cgttagtttc tgctactcct tcgctgtcat ctacagagaa atccggcgtt gagttcgggt
    25441 tgctcagcag caactcacgt actttcttct cgatctcttt cgcggtttcc gggttatctt
    25501 tcagccaggc agttgcattc gctttaccct gaccgatctt ctcacctttg tagctgtacc
    25561 acgcgcctgc tttctcgatc agcttctctt ttacgcccag gtcaaccagt tcgccgtaga
    25621 agttgatacc ttcgccgtag aggatctgaa attcagcctg tttaaacggt gcagcgattt
    25681 tgttcttcac cactttcacg cgggtttcgc tacccaccac gttttcgccc tctttcaccg
    25741 cgccgatacg acggatgtcg agacgaacag aggcgtagaa tttcagcgcg ttaccaccgg
    25801 tagtggtttc cgggttaccg aacatcacac caattttcat acggatctgg ttgatgaaga
    25861 tcagcagcgt gttggactgc ttcaggttac ccgccagctt acgcatcgcc tggctcatca
    25921 tacgtgccgc aaggcccatg tgagagtcgc cgatttcgcc ttcgatttcc gctttcggcg
    25981 tcagtgccgc cacggagtca acgacgataa cgtctactgc gccagaacgc gccagggcgt
    26041 cacagatttc cagtgcctgc tcgccggtat ccggctggga gcacagcagg ttatcgatat
    26101 cgacgcccag tttacgtgcg tagattgggt ccagcgcgtg ttcagcatcg ataaacgcac
    26161 aggttttacc ttcacgctgc gctgcggcga tcacctgcag cgtcagcgtg gttttaccgg
    26221 aagattccgg tccgtagatt tcgacgatac ggcccatcgg cagaccacct gccccaagcg
    26281 cgatatccag tgaaagcgaa ccggtagaga tggtttccac atccatggaa cggtcttcac
    26341 ccaggcgcat gatggagcct ttaccaaatt gtttctcaat ctggcccagt gctgccgcca
    26401 acgctttctg tttgttttcg tcgatagcca tttttactcc tgtcatgccg ggtaataccg
    26461 gatagtcaat atgttctgtt gaaacaatta tactgtatgc tcatacagta tcaagtgttt
    26521 tgtagaaatt gttgccacaa ggtctgcaat gcatacgcag tagcctgacg acgcaccgca
    26581 tcacggtcgc cgctgaagca ttcccgccgg gtaatgcctt caccgcgggc agtggcaaaa
    26641 gcaaaccaga cggtgccgac aggcttctct tcactgccgc catccggccc ggcgatacca
    26701 ctaatagaca cggcataatc agcacgagcc gctttcagtg cacctatcgc catttccacc
    26761 acgacgggtt cactcaccgc gccatgctgc gccagcgtct cttcgcgtac gccgatcatc
    26821 tgcgctttgg cttcgttact gtaggtgaca aatccgcgtt caaaccaggc ggagctaccg
    26881 gcaatatcgg taatcacttt cgctacccaa ccaccggtac aagactcggc agttgttacg
    26941 gttgcgccac gggctttcag cgcctgccca acctgttcgc ttaactgcat cagttcactg
    27001 tcagtcatca caacctctgt cagtcaaaaa tttatgccgg acaagatagc actttcgtca
    27061 ggaagatggt gttggcgttg caattttacg aggaggattc agaaaaaagc tgattagcca
    27121 gagggaagct cacgcccccc tcttgtaaat agctactgta ctcgcgccag cgccacggct
    27181 tgtcctaact gccagaccgc cattgcgtaa tgggtgctgt ggttgtaacg ggtgatggtg
    27241 tagaagttcg gcagaccgta ccagtactgg tagccggtgc caacatccag acgcagcagg
    27301 ctggcttgtt gatggttgcc cagcggctgc tgtggcgtta aacctgcggc ggcaagctgc
    27361 gaaatgctgt acttagtttt gaaaccattt ggcaagcctg gagcctgacc gtttgccatt
    27421 atcgcgacct gatcgccttt cacccagccg tgcgctttga aatagttcgc cacgctaccg
    27481 atcgcatcaa ccggatccca caggttgata tgcccgtcgc cgctgaaatc taccgcatat
    27541 tgtttgtaag acgacggcat aaactgtccg tagcccatcg ccccggcaaa ggaacctttc
    27601 agattgagcg gatcgtcctg ctcgtcgcgc gccatcagca ggaaggtttc cagctcgcca
    27661 gagaaatact ccgcgcggcg tgggtagtta aatgacagcg ttgccagcgc atcgaggatg
    27721 cgagttttcc ccatcacgcg cccccagcgg gtttcaacgc cgataatccc gacgataatt
    27781 tccggcggta cgccatacac ctgccacgcg cgattcaacg catcttcata ctgattccag
    27841 aaaaccacac cgttctgcac gttgtccggc gtaataaatt ttttgcgata acgcagccat
    27901 gcgccgttcg gacctgatgg cggtttcacc gatgtggttg gtgcctggtt atccatcagc
    27961 cgcagtaccg aatccagacg cttcgcctgg gagagaattt cctgcaactg ctgacgatcg
    28021 aaaccgtgtt tgttcaccat tttgtcgatg aactgctggg cattcgggtt attagcgaaa
    28081 tcgccgccca tctgcatcac attgtgctgc ggctcaagca ggaagccgcc agacggcgtt
    28141 ccggtggtcg tttcagtctc agtaggtttt ggcttgctgc tacaggcggc aagcaacaca
    28201 aaaaggggaa gcaatgttac ataacgacgc ttgaacatga ggggtccatt taacagattc
    28261 aaccaggggc aagtatggta aagcatcaca ccccgcacaa ggaagcggta gtcactgccc
    28321 gatacggact ttacataact caactcattc ccctcgctat acttttattc aaactttcaa
    28381 attaaaatat ttatctttca ttttacgatc aaaataacac ttttaaacct ttcaacctga
    28441 ttagattagg ttgccgtttg gtaataaaac aataaatcct gaaggagaga acaatgatag
    28501 aaaccattac tcatggtgca gagtggttta tcgggctgtt ccaaaagggc ggagaggtgt
    28561 ttaccgggat ggtgaccggc attcttccgc tgttgattag cctgcaggtt atcatgaacg
    28621 cactgattaa ttttatcggt cagcatcgta ttgaacgttt tgctcaacgt tgcgccggta
    28681 accctgtttc ccgttaccta ctgttaccgt gcattggcac gtttgtcttt tgcaatccga
    28741 tgaccttaag cctgggtcgc tttatgccgg aaaagtacaa acccagctac tacgcggcgg
    28801 cctcttatag ctgccactca atgaatggcc tcttccccca tatcaaccct gcgaactgtt
    28861 tgtttatctt ggcattgcca gcggtctgac aacgctgaac ctgccacttg gcccactggc
    28921 ggtgagttat ctgctggttg gtctggtcac caatttcttc cgcggctggg tgaccgatct
    28981 gaccaccgcc atttttgaga aaaagatggg cattcaactt gaacaaaaag ttcacctggc
    29041 aggagcaaca tcatgacgcg tattcggatc gaaaaaggaa cgggtggctg gggcggcccg
    29101 cttgagctgg aagccacgcc gggcaaaaaa atcgtctata tcaccgccgg tacccggcct
    29161 gcgattgttg acaaactggc acaacttact ggctggcagg ctattgacgg atttaaagaa
    29221 ggtgaacccg cggaggcgga aattggtgtc gcggtaatcg actgtggcgg cacattacgc
    29281 tgcggcatct atccgaaacg acgtattccc accattaata tccactcgac gggcaagtcc
    29341 ggtccgctgg cgcagtacat tgtggaagat atttatgtct ctggcgtaaa agaagaaaac
    29401 atcactgtag tgggtgatgc gacaccacaa ccctcttccg tgggccgtga ctatgacacc
    29461 agtaagaaaa tcaccgaaca aagcgatggt ttactggcga aggtgggaat gggcatgggg
    29521 tccgccgttg ccgtgttgtt tcagtctggt cgtgacacca tcgacactgt attaaaaacc
    29581 attctgccgt ttatggcatt cgtctcggcg ctcattggca tcattatggc ttctggcctt
    29641 ggtgactgga ttgcccacgg tcttgctccg ctagcgagcc atccactggg tctggtcatg
    29701 ctggcgctca tctgctcctt cccactgctt tcacctttcc tcggcccagg cgcagttatc
    29761 gcacaggtta tcggcgtatt gattggcgtg cagattggtc tcggcaatat tccgccgcat
    29821 ctggctttac cggcactgtt tgccatcaac gcgcaggcgg cttgcgactt catcccggtc
    29881 ggtttgtcgc tggcggaagc ccgtcaggac acggttcgcg tcggtgtccc ttctgtactg
    29941 gtgagccgct ttttaaccgg cgcgccgact gtactgatcg cctggtttgt ctccggtttt
    30001 atctatcaat agaggctgaa acatgaccgt tatttatcag accaccatca cccgtatcgg
    30061 cgcgagtgcc actgacgccc tcagcgacca gatgctcatc acctttcgtg aaggcgcgcc
    30121 agcggacctc gaagagtatt gcttcattca ttgccacggc gagttgaaag gtgcactcca
    30181 tcccggtttg caattttcac tcgggcagca tcgctatccg gtgaccgctg ttggcagcgt
    30241 ggcggaagac aaccttcgcg aactgggtca tgtcaccctg cgcttcgatg gtttaaacga
    30301 agcggaattt ccgggcactg tccatgtggc aggccctgta cccgacgata tcgcgccggg
    30361 atcggttttg aagtttgaat ctgttaagga gtaaaaaatg aatcaggttg ccgttgtcat
    30421 cggtggtggg caaaccttag gcgcgttcct gtgccacggt ctggctgccg aggggtatcg
    30481 cgtcgcggtt gtcgatattc agagcgacaa agccgcaaat gtggcacaag aaattaacgc
    30541 cgaatatggt gaaggtatgg cgtacggttt tggtgctgac gccactagcg agcaaagcgt
    30601 tctggcgctc tctcgtgggg tagatgaaat ctttggtcgc gtggatttgc tggtctatag
    30661 cgccggaata gcaaaagcag cctttatcag cgacttccag ctcggcgatt ttgaccgttc
    30721 gctacaagtg aatctggtgg gttatttcct gtgtgcccgt gaattttcgc gtttgatgat
    30781 ccgcgacggg attcagggtc gcattattca gatcaactcg aaatccggca aagtgggcag
    30841 caaacacaac tctggctaca gcgcagcgaa atttggtggc gtcgggctga ctcaatcact
    30901 ggcgctggat ctggcggagt acggcattac ggtgcattca ctgatgctcg gtaacctgct
    30961 gaaatcgcgg atgttccagt cactgttgcc acaatacgcg accaagctgg gtatcaaacc
    31021 ggatcaagtc gagcagtatt acatcgacaa agtaccgctc aaacgcggct gcgattatca
    31081 agatgtgctg aatatgctgc tgttctacgc cagtcctaag gcgtcgtact gcaccggaca
    31141 gtcgatcaat gtcaccggcg gtcaggtgat gttctgatca acagcggaga tccgttaagg
    31201 atctccgtga gactatagaa tgcctgatgc gctacgctca tcaggcatac aggacttccg
    31261 ccactacatt aaggaaaagt tatggtatcc gcactcatca ccgtcgccgt tatcgcctgg
    31321 tgtgcgcaac tggccttagg cggctggcaa atttctcgtt ttaaccgtgc cttcgacaca
    31381 ctatgccagc aagggcgggt tggcgtggac cgttccagcg ggcgctttaa accgcgggtc
    31441 gtggtcgcca tcgcgctgga cgatcagcag tgcatcgtcg acaccttgtt tatgaaagga
    31501 ctgactgtct tcgcccgacc gcaaaaaatt cccgcaatta ccggcatgca tgcgggtgat
    31561 ttacagcccg atgtgatctt tccccatgat ccactatcac agaatgctct atcattggcg
    31621 cttaaactga aacgtggata atttcgttgt gaatgttact tgcttgcgaa gttatcattt
    31681 tgaaacctaa atcaggtaat cacgcccatg aaacctcgtc agcgtcaggc cgccattctg
    31741 gagtatctgc aaaagcaggg taaatgctcg gttgaagaat tggcgcaata ctttgacacc
    31801 acaggcacaa ccattcgcaa agatctggtc attctggaac atgccggaac cgtcattcgt
    31861 acttatggcg gagtggtgtt gaataaagag gaatccgatc cgcctatcga tcataaaacg
    31921 ctcatcaaca cccacaagaa agagctgatt gcagaagctg ccgttagttt tatccatgat
    31981 ggcgattcga tcattcttga tgctggcagt accgttttgc agattgttcc cctgctctcg
    32041 cgctttaata acatcacggt gatgaccaac agcctgcata tcgtcaatgc gctatccgaa
    32101 ctggataacg aacaaactat cctgatgcca ggcggaacat ttcgcaaaaa atcggcctca
    32161 tttcacgggc aactggcaga gaatgccttc gagcatttca ccttcgataa attgtttatg
    32221 ggcaccgacg gcatcgatct caatgcgggc gtaaccacgt tcaacgaggt ttataccgtc
    32281 agtaaggcaa tgtgcaatgc cgcgcgcgaa gtgattttga tggcggactc atcaaagttt
    32341 ggccgtaaaa gccccaacgt agtttgcagt cttgaaagcg tcgataagct aattaccgac
    32401 gcaggtatcg atccggcgtt tcgtcaggcg ctggaagaga aagggatcga tgtgatcata
    32461 accggagaga gcaatgagtg aagcactact gaacacggga cgtcagacgt taatgctgga
    32521 gttgcaggaa gcaagccgtt taccggaacg tctgggcgat gattttgttc gcgccgccaa
    32581 tatcatcctg cactgcgaag gcaaagtggt ggtttcggga attggcaaat cgggccacat
    32641 tggtaagaaa atcgccgcaa cacttgccag taccggcaca ccggcttttt ttgtccatcc
    32701 ggcagaagca ctgcacggcg atctggggat gattgaaagc cgcgatgtga tgctgtttat
    32761 ctcttactcc ggtggcgcga aggaactgga tctgattatt ccgcgtctgg aagataaatc
    32821 tatcgcgctg ctggcgatga ccggcaaacc gacgtcaccg ctgggcctgg cggcgaaagc
    32881 ggtgctggat atctccgtag aacgcgaagc ctgcccgatg caccttgcgc cgacctccag
    32941 caccgtcaat accctgatga tgggtgacgc gctggcgatg gcggtcatgc aggcgcgcgg
    33001 ttttaatgaa gaagattttg cccgctccca cccagctggg gcactgggcg ctcgcttgct
    33061 gaataaagtg catcatctga tgcgtcgtga tgacgctatc ccacaggtag cgttaaccgc
    33121 cagcgtgatg gatgcgatgc tggaactcag ccgcaccggt ctggggctgg tggcggtatg
    33181 tgacgctcaa caacaggtac aaggcgtctt taccgacggc gatttacgtc gctggctggt
    33241 aggcggcggc gcactcacca cgccagttaa tgaagcgatg acgacaggcg gcaccacatt
    33301 acaggcacaa agtcgcgcca tcgacgccaa agaagttctg atgaaacgca aaatcactgc
    33361 cgcaccagtg gtagatgaaa acggcaaact caccggcgca attaacctgc aggatttcta
    33421 tcaggccggg attatttaat ccttcaatcc cagacgtttc gccagccgat gcaggttggc
    33481 gacgtcagtt tccagcattc gcgcacaggc tgcccagttg tgatgatttt gtgccagtgc
    33541 ctggcgaata gtttcacgct ggaacgcttc tgtcgcttca cgcaggtttt gcttaacaac
    33601 gggcaccgcc gccgcttctg gcggcggcaa cgtcacctca ggaaaagcaa aatgttgcgc
    33661 ctcaagaatc acttcatcgc cgttgcgggt ggctctcgcc agcactaccg cccgatgaat
    33721 agcatgttcc agttcgcgca cgtttcccgg aaaactgtag tgttgcagta aatttcgcgc
    33781 cccggcactt aataccacgc gggagagccc ctgccgcaaa cgacactgct cgcagaaata
    33841 ccccgccagc agaatgacat catcgccccg ctcacgcagc ggcggcaccg aaagtggaaa
    33901 cacgctcagg cgatgaaaca aatcggcgcg gaatcgccct gccagcacct cttcgcgtaa
    33961 gtcgcggtta gtcgccgcca gcacgcgcac atcgacccgc aaactacggt catcgccaac
    34021 gcgctgaata tcgccatact gcaacaccct cagcagcttg gcctgcaatg ccaacgacaa
    34081 ctcgccgatc tcatcgagaa acagagtacc gttatccgcc atttcaaact tcccgctgcg
    34141 gttactgata gcgccagtaa acgctccttt cacatgcccg aacaactcac tttccgccac
    34201 actttccggc agtgcggcac agttgagata gaccagcgga ttcaccgccc gtggcgaggc
    34261 ttcatgaatc gctttcgcca ccagctcctt accggttccc gtctcaccgc tgatcagaac
    34321 gttgagatcg gacgccgcca caatctcaat ctcttttttc agttgcgtca tgccagggga
    34381 caagccaatc atctgcgtct gtttcaccgc ttcaaacggc gtggcatcgc ctggcagcat
    34441 attctggctt tccagttgtt caatcagcaa cgcattgctt aacgctcccg ccgccagcgc
    34501 ggcaatcagc cgtaactctt cgtcgctgaa aacatcgaac tgatcgggct gcatcccgtc
    34561 gagcgtcagt gcgccgatca ggttttgtcc gacaaacaat ggcagaccaa cgcaggcgtg
    34621 aaccttcaga ctctcctgcc caggaatcaa accgtcatag ggatcgggca attcgctgtc
    34681 tgcgggaaag cgcaccacat ccccggcgcg ggcaatcgct tccagccgtg gatgcccttc
    34741 cagcgcaaag cgtctaccga gtacatcctt tgccagaccg tcgatggcaa gcggaataaa
    34801 ctgccgcgaa tcgtaacgta gcaacgcaga cgcatcgcac tccagcacct gacgtagcgt
    34861 ggtgatcagg cgctgaaaac gatcctgatg accaatccca cgctgcaatt cgatggcgat
    34921 attcgccagc acatcaacgg aaaaactcat ctttgcctca ctgtcaattt gactatagat
    34981 attgtcatat cgaccatttg attgatagtc attttgacta ctcattaatg agcataattt
    35041 tatttataga gtaaaaacaa tcagataaga aactggcacg caatctgcaa ttagcaagac
    35101 atctttttag aacacgctga ataaattgag gttgctatgt ctattgtggt gaaaaataac
    35161 attcattggg tgggtcaacg tgactgggaa gtgcgtgatt ttcacggcac ggaatataaa
    35221 acgctgcgcg gcagcagcta caacagctac ctcatccgcg aagaaaaaaa cgtgctgatc
    35281 gacaccgtcg accataaatt cagccgcgaa tttgtgcaga acctgcgtaa tgaaatcgat
    35341 ctggcggata tcgattacat cgtgattaac catgcagaag aggaccacgc cggggcgctg
    35401 accgaactga tggcgcaaat tcccgatacg ccgatctact gtactgccaa cgctatcgac
    35461 tcgataaatg gtcatcacca tcatccggag tggaatttta atgtggtgaa aactggcgac
    35521 acgctggata tcggcaacgg caaacagctc atttttgtcg aaacaccaat gctgcactgg
    35581 ccggacagca tgatgactta cctgacaggc gacgcggtgc tgttcagtaa cgatgctttc
    35641 ggtcagcact actgcgacga gcatctgttc aacgatgaag tggatcagac ggagcttttc
    35701 gagcagtgcc agcgttacta cgccaatatc ctgacgccgt tcagccgcct ggtaacgccg
    35761 aaaattaccg agatcctggg ctttaactta ccagtcgata tgatagcgac ctcccacggc
    35821 gtggtctggc gcgataaccc gacgcaaatt gtcgagctgt acctgaagtg ggccgctgat
    35881 tatcaggaag acagaatcac cattttctac gacaccatgt cgaataacac ccgcatgatg
    35941 gctgacgcta tcgcccaggg aattgcggaa accgacccac gcgtggcggt gaaaattttc
    36001 aacgtcgccc gaagcgataa aaacgaaatc ctgaccaatg tcttccgctc aaaaggcgtg
    36061 ctggtcggca cttcgacgat gaataacgtg atgatgccga aaatcgccgg gctggtggag
    36121 gagatgaccg gattacgctt ccgtaacaaa cgcgccagtg ctttcggatc tcacggctgg
    36181 agcggcggtg cgatggatcg cctttccacg cgcctgcagg atgcgggttt cgaaatgtcg
    36241 ctcagcctga aagccaaatg gcgaccagac caggacgctc tggagttatg ccgtgaacac
    36301 ggtcgcgaaa tcgcccgtca gtgggcgctc gcgccgctgc cgcagagcac ggtgaatacg
    36361 gtagttaaag aagaaacctc tgccacgacg acggctgacc tcggcccacg aatgcagtgc
    36421 agcgtctgcc agtggattta cgatccggca aaaggcgagc caatgcagga cgttgcgcca
    36481 ggaacgccgt ggagtgaagt cccggataac ttcctctgcc cggaatgctc cctcggcaaa
    36541 gacgtctttg acgaactagc atcggaggca aaatgagcaa cggcattgtg atcatcggtt
    36601 cgggcttcgc cgcccgccaa ctggtgaaaa atattcgcaa acaggacgcc actattccat
    36661 taaccctgat tgccgccgac agcatagatg agtacaacaa acccgacctc agccatgtta
    36721 tcagtcaggg gcaacgtgcc gacgacctta cccgccagac ggcgggtgaa tttgccgggc
    36781 agtttaatct gcgcctgttt ccgcacacct gggtgacgga tatcgatgcc gaagcccatg
    36841 tggtgaaaag ccagaataat cagtggcaat acgacaagtt agtgctggca accggtgcca
    36901 gcgcctttgt cccgcctgtg cctgggcgtg agttaatgct gacgttaaat agtcagcaag
    36961 agtatcgcgc ctgtgaaacg caactgcggg atgcccgacg cgtgttgatt gtcggcggtg
    37021 gtttgattgg tagcgaactg gcgatggatt tttgtcgtgc aggcaaagcg gtcacgctaa
    37081 tcgacaacgc tgccagtatt ctggcgtcat taatgccacc agaagtaagc agccgcttgc
    37141 agcatcggtt gacggagatg ggcgttcatc tgctgttgaa atctcagttg caggggctgg
    37201 aaaaaacgga ttctggcatt ctggcaacgc tggaccacca gcgtagtatc gaagtggatg
    37261 cggtaattgc cgccaccgga ctgcgcccgg aaacagccct ggcacgacgc gccgggctga
    37321 cgattaatcg cggcgtttgc gtcgatagtt atctgcaaac cagtaatgcc gatatttacg
    37381 cgctgggcga ttgcgcggaa attaacggtc aggtattgcc gttcctgcag ccgattcaac
    37441 ttagcgggat ggtgctggca aaaaatcttc tcggcaataa cacgccgctg aaactaccgg
    37501 cgatgctggt gaaaatcaaa acgccggaat taccgctgca tctggcaggc gaaacccagc
    37561 gtcaggattt acgctggcag attaataccg aacgccaggg aatggtggcg cgcggcgttg
    37621 acgatgctga ccagcttcgc gcctttgtgg tcagtgagga tcggatgaaa gaggcatttg
    37681 tattgttgaa aacgttgccg gtgtaggtgg gctactgtgc ctaaaatgtc ggatgcgacg
    37741 ctggcgcgtc ttatccgacc tacggggacg catgtgtagg ccggataagg catttacgcc
    37801 gcatccggca agggtgtcca aatgcaacac gctttatccg ttctggactt cacccgctaa
    37861 ccaacgcgcc gcagcaataa ccccctgccc cagagacaaa ccgccatcac ccgccggtaa
    37921 actctgtgga aagagcaatg tgaaatcagc gagataatgc gccagacgtg cacgcagcaa
    37981 acggttatga ataaccccgc cgctaaatac cagcgtagtg ataccacgca tcgtggcctg
    38041 ctcacgcatc aacgcggcaa aaccctgcgc cagcgcatca tgaaacgccc acgcgcgttg
    38101 attaaccagt gcctgccagt tcagccactg ctgccagaaa gtggcgagat ccagttgatt
    38161 gtccacccgc ggcatcgtca ccggatgcgt cactccgtcg catgaggctg cgagcgcctc
    38221 cagagcacaa gccgcttcac cttcataact taacgtggct ggcgcacagc ccagtgccgc
    38281 cgccactgca tcgaacaacc gcccacacga tgatgccagc ggcgcgttaa ttccacgctc
    38341 aatggcccgc gccagtacgc tccagttttg ctgttgcaca cttgccgttt cagggtaatt
    38401 ctgccactcc ggcacaaagc gcaggcactg cgccagcagg tttcgccacg gctgcttcgc
    38461 cgccaaatcg ccacccggaa gcgccactgc aggcaagccg cccaggtgct cacattcgcg
    38521 atagttcacc cgcaggcact cgccgcccca caaagcgcca ttctccccca taccgatacc
    38581 gtcgagcgtc aaagcaatga catcaccgcc atccagcggc cactgatgct ctgccagaca
    38641 cgccgctgca tgggcatgat gatgcagtac cgtttgcgtc ggcagattca tttcgctcgc
    38701 ccactggctg gagacatagc ccggatgcac atcatgcaca atgtattgcg gagtgaaatc
    38761 gtagatgttt tgcatcaggc gtaacgcttc gcgccactgc atctggatgc catcgtcact
    38821 taaatcgccc agatgctgac tcaacaccgc ttgttcaccg cgcaccaggc agaaggtatt
    38881 tttcagatcc gcgccaagac acagcacagg cggaacattt ttaaagcccg gaggcaaaac
    38941 cagcgcatcc ggcacatacc cccgcgaacg gcgcagcatt tcgccgcttt cgcgcaccac
    39001 tgaatcatcc atccgctgca cgatgtcgcg gttatgtagc aagaatccgt cggcaatgcc
    39061 ctgcaaatcc gccagcgcct gttcgttgct gatagctggt ggtttaccgc tcaggttgcc
    39121 ggaggtcatc accagcgggc attgcagttc ctgtaacagc aaatgctgga gcgggttcgc
    39181 aggcaacatc accccgactt cgttaaggcc aggggcgata tcatcacaaa gctcaggtac
    39241 gtatttttta tccaccagca caatcggcgc agcgggcgtg gtaagcaact ggcgcgcagc
    39301 gtctggtaaa ctttctgcca ctggcaacat gactgccagc ggtttcgccg ggcgatgttt
    39361 gcgctcccgc agtgtcgcca ccgcgttact gttacgtgca tcgcaggcaa gatgaaatcc
    39421 gccaatccct ttgatggcga caatgttgcc catttttaac tgtgcgatag ctgcctgtaa
    39481 tgccgcctct tgttctgcat gttcaccatg acttacccat tcaagatgcg ggccacactc
    39541 cgggcaggcc accggctggg cgtggaagcg acgatcgagc gggtcgcggt actctttgtc
    39601 acaggccgga catagcggaa acgccgccat cacggtaaac gggcggtcgt aaggcatcgc
    39661 gcgaataatg gtgaaacgcg ggccgcagtg ggtacagttg ataaacggat aacgataacg
    39721 ccgctcgcct ggggtattca tttcggcaag gcaagcaggg caagtggcgg catccgggac
    39781 aatttgcgta ttcatggtgc cgccggtgct ctggcggatg gtgaactcgg tgggcagttg
    39841 tgaccagata aacggctcac gctcgacgct atcaatacgc gccagtggcg gacagtgctg
    39901 atgcaattga acaagaaacg tttccgggtc ttccagcagc cggacttcta cgccatcgcc
    39961 gtcattacat acatcgccgt gaagatttaa ttgctgtgcc agctgccaga caaacggacg
    40021 aaaaccgacg ccctgcactt tgccacgaat acgcagttgg acaccgcaag atgtgttttt
    40081 tgccattgag ttattcccgc catcatgaat tgcgtaaccc gccctgccgg acacgacagc
    40141 gtcgtatccg gcagtctcag gtcggcgata ccgcccgctc cgtattctac gaatatttcc
    40201 gggaattcct ttgatgccag aacagttctg taagattttt agaacatcag cgccgtacgg
    40261 cggcgttttt ctgcgctcag ttgttcaagt ttattacgat cgacacaaat cagcgcatgg
    40321 gtcgggcaag ccgccataca cgccgggccg tcttcacgat ggttgcacag gtcgcattta
    40381 ttggcttcgg ctttgtcagc ccgtacattc agacccgcgc cgctgttgcg aatcacagga
    40441 cgtaccacca cttccatcgc gccatacggg caagccacca cgcaggtttt gcaaccaatg
    40501 caacgttcct gcatcacagg aacaaaccct ttatcacggc tgatagcacc attcgggcag
    40561 acgttagcgc acggtgcatc ttcacactga cggcaaactg tcgccgtgga aatgttcaca
    40621 cctttaatga catggatacg cggtaaaaat gtttccgggg tcagcgatgc acagtcctga
    40681 ttttcctgat gagaaaccac gcacgccact tcacaggtac ggcaaccaat acatttactc
    40741 gcgtcagcaa tgatgaaacg gttcatcaaa ttctccagca atgacagtta atgcgccgat
    40801 acactcacaa atcatgccag tttttaattt gctgttattt aaggaaatta atttctgtaa
    40861 tgcaggaaaa aagatgtcat cgacactagt gacgatgaca tgtgatgaca atgtttatcg
    40921 cgaaagggca atgagtgagt cgcggcggat cagttttccg ctaaaggttt tcggcggtga
    40981 gaaatccccg ccatcgagca taaaaatcag ccgtccaata atttcctgaa tcatctcagt
    41041 caccggaatt tttacgctgg agagcgccgg aacgatatag ggggcaattg cgatatcatc
    41101 gaatccgata actgacacct gctctggcac cgctacgccg cgctcgtgca acgctttgat
    41161 cgcacctatc gccatatcgt cgttactggc aactaacgcg ctaaatttag cccctcgttc
    41221 gagcaacgtt tctacccctt cggccccgct ggcgggcgtc catttaccgt tagcgataag
    41281 tttttcattg agcgcaatac catgctgcga cagcgcgtct ttatacccgg caagacgttc
    41341 aatgctggtg ggggaatcca tcgagccggt aaggaaagca atctcctgat gcccggcgtt
    41401 tatcaactct gccacggcgt taaagctggt ctgtttatga tcgcaccaga cgctatggct
    41461 gctattattg cgcaggcggc gattaagcac cattatcggc tgactgtgcg cgtcaatgat
    41521 gtcatcaatc tcatccacgc ttaaaaaacg cgggtaaatc ataatggcgt cgcagcgcaa
    41581 atccagcaga tactgaatcg cctggcgctc ttcttcggcg ctgtgtttac catctgccaa
    41641 tagcaactgt cgccctttct cttccgccat tcgcgcggca tgaaacagta attcactaaa
    41701 ataaatgccg tgataaagcg tgttggtcac taccagcccc agcgtctgag tactcttcgc
    41761 cgacagattg cgggccagca aatttgggcg gtaactgctc tcttctaccg cctgaaacac
    41821 gcgatcttta gtctcctggc tgacgtagcc attacctgaa agcacgcggg aaacggtcgc
    41881 ttttgaaacc ccggcgcgct tcgccacttc cagcatcgtc gtcatcattt tcatcccttt
    41941 acacgcaatc aacgcagtgt actgcaccgt ttgccgattg tccttgcgca atcggctcca
    42001 gcggataaga cgaggtcgcc gtgccggttt tgatgacttt tttgataaag gtaaacatgg
    42061 cgattcctta tttcagcggc gagtttttac gctcaatgct gtaacgctcg agttctttgt
    42121 acggcaccac tttgctcttc ttcttacgca catcgactac ggtcatgcgg tcggtacagg
    42181 agtagcaagg gtccaggcta ccgataatca gcggcgcata ggaaacggta ttgccacgca
    42241 gcatgtagcg cagggtcggc cagttcgcgt aggtcgcggc acggcagcgc cagcggtaca
    42301 gcttctggtt gtcgccggtc atgctccagt ggatatcgtc gccgcgcggc gcttcggcaa
    42361 agcccagcgc gaaacggtgc ggaatgtagg taaagccttc caccatcagc ggaccgcccg
    42421 gcaggttatc cagaccgtag tcgatcatat tcagcgcggt atagacctcg ttgattcgca
    42481 ctttcagacg ggaaataacg tcgcagccct gctcgctgtg gacttccatt ggcagcaggc
    42541 cataaccgac aaacgggtga tcggcgcgag tatcgcgggc gagaccgctg gcgcgaacca
    42601 tcgggccaac gttactgaaa tcgcgggcga tttccgggtc cagacgacca atgccgacgg
    42661 tgcgttgttc catgttcggc gtactcagca gcacatccac cagctcctgc acttcacgac
    42721 gcatttgttg cgccagctgg cgggtctgga tcatgtcatc tttcagcaga tcgcgacgaa
    42781 tcccgccgat caggttcagg ccgtaggttt tacgcgcacc ggtaaggatc tctgccattt
    42841 tcatggaggt ttcacgcacg cggaagaact gcataaagcc ggagtcgaag ccggtaaagt
    42901 gacaggcgag gccgaggttg agcagatgcg ggtgcaggcg ttccacctcc agcagaatgg
    42961 cgcggatcat ctgcgcgcgt tctggcacct gaatacccat cgcgttttcc accgacgtgg
    43021 tgtaggcggt gctgtgggca aagccgcaga tcccgcacac acggtcagag aggaaggtca
    43081 cttcgttata acccatgcgg gtttccgcca gtttttccat accgcgatgg acgtagaaca
    43141 gacggtagtc ggcgtcgata atgttttcgc catcgacgaa cagacggaag tgacccggtt
    43201 cgtcagaagt gacgtgcagc ggaccaatcg gcacgacgtt gtttttcttg tcgcccagtt
    43261 cgttgatgaa ctcgtaggtt tcagcatcgg tagtcggtgc cggacgctga cgataatcca
    43321 tgctgtcttt acgcagcgga taaagttcat ccggccagtc atccggcagc accagacgac
    43381 gttcatccgg cagaccaacc ggaatcaaac cgtacatatc gcgcacttcg cgctcgcccc
    43441 acaccgccgc cggaacgcgc ggcgtcacgg acggatactc cggtttgttg gcgtcaactt
    43501 cgacgcgaac cgttacccaa cacttggtgc ccttctccat cgacagcacg taataaacgg
    43561 cgtaatgacc attcagtttg cgttcgtcgt taccaaacag caccgacagc cagccaccct
    43621 gcttgtagta gagaaactcc accacttccg gcagatagtt cacctttacg gtgacagtca
    43681 gctgatcttt ggtctgccag gcgtggtcca gcacgacgcc tagaaatgcc tcattcagcg
    43741 cggcgagata atgttgacct aatttttctt cagacatgct caaactctct ttaatcacgc
    43801 cgccagcaag gagacgaacg ctaaaaatgc aaagccaaac ccagcccagg taatgcgcgg
    43861 agtaatatca agacgcagac gggccatgct gttttcgaac agcgcgataa ccaggacgcc
    43921 gaccaccagt ttgacgatgg caatcaccag cgccagcagc agtccaccga cggtgaaggt
    43981 ttccatttgt ccccacggaa taaacacccc gacgaacatc tgcaacacca ccagctgttt
    44041 caggctgata ccccatttca tgacgccaaa gccgctaccg ctgtattcgg agagcggacc
    44101 ttcctgcaac tcctgctcgg cttccgccag gtcgaacggc agtttgccca tttcgataaa
    44161 agtggcgaac gcacaggcgc agagcgccag caccagcggg atgctttggc tcagcggcca
    44221 gtgataaacg gtgtcggtaa tgttgctgat gttggtggaa ccggcaacct gtgcggcaac
    44281 ccacagacca agcagcaaca tcggttcgac cagcacgcca agcatcgctt cacggctcgc
    44341 accgatagcg gtaaacgggc taccggtatc cagaccggaa atggcaaaga agaaacgcgc
    44401 gatggcgaag agatacagta aggtgatcaa atcgcccagt tgcggcagcg gagaaccgac
    44461 ggtcaccacc ggcagcgcag tagcgatagt cagcatgacg cccaccatca catacggcgt
    44521 caggcggaac atccagccag aggcatccgg gccaacgctc tggcggccca gcagtttgat
    44581 aatgtcgcga tactcctgca acacgcccgg cccgcgacgg ttatgcagac gggcgcgcgc
    44641 cacgcgggta ataccggaga gcagcggcgc aacggcaaat aacaccagcg cctgaattaa
    44701 cggatataaa acactcattc tcaggctcct cgtgaaacaa taatcaccac cagtaccgcc
    44761 agttcaacca gcgccatccg gcggaacagc aacgcactac cttcgcactg ccagcccggc
    44821 actagcgaca ccggattcag ccatttgcgc agtttcagca ctggcgcaaa cgcctgttta
    44881 accggcatgg caaaaccgtg agcggtaatc accattgatt tttcatggtc gtagccgcac
    44941 acccaggccg caccgcggga acgtgacggc aggcggtcgc ctttgcaaat cgccataatg
    45001 atgaatggca gcagcgggca ggcaatcagc agcaatgtga tcattggttg agaaacggtg
    45061 gtgttagcag gctccagagg cagaggtaca gcagcagaga gcatcggcag cagccacggc
    45121 gcagcaacac cgccaattac gcagcaaatc gccagtgcca ctacgcttac gctcatcagg
    45181 agcggcgcac aggtggcgtt ttcggcttct ttggtacgcg gcgcgccgag gaacgtgacg
    45241 ccatagactt tcgccataca catcaccgcc agcgcaccgg taattgccag acccacagcg
    45301 agcagcggcc ccagcagacg ggcaacaaac gcgccgctat tgctcagttt gaagaaggat
    45361 tgatagataa cccattcccc ggcaaacccg ttcagtggcg gcagcgcagc cattgccatc
    45421 aacccgacta acatggcgat ggagataacc ggcattttct tgccaatacc accgagtttt
    45481 tcgatatcgc gatgaccggt acggaaccag acgctccccg ccccgaggaa cagtacgctt
    45541 ttgaacaggc tatggttaag cagatggtac agaccaccga ccaggccaag agcaatcagc
    45601 gccggttgtt cgagcgcgat acccgttacg ccagcgccca gccccagccc cagccccagc
    45661 cccagcccca gccccagccc cagccccagc cccagcagga taatgccgat attttccagg
    45721 gtgtggtaag ccagcaggcg ctggatgttg tgttccatca acgcatacag accaccgacg
    45781 aacgcggtga tcatgccgag caccagcagc gcgatccccc accacagcgg tgcattaccg
    45841 cccagcagtg acagggttaa catgcccagc aggccaattt tcatgactac cgtagaaaac
    45901 aacgcggcag ctggcgcaga ggcgttagca tgtgcctgcg gcacccagcc gtgcagcgga
    45961 ataatcccag ccagcaggcc aaagccaatc acaccgagca gccagatatc ggaaccgagc
    46021 ggcagctgtt gcatacgcat atccagcagg cgcagatcca gcgtgccata acgctgccac
    46081 agcagccagc aagcaatcac cagcagcaga gtgccaagac gcctcagcgc aaaccacagt
    46141 ttgccctctt tgctgttgct ggtgaggaac accgcacaca aggccatgac ttcggccatt
    46201 accacgaaca tgccgaggtt gctggcgatg acggcgcaga cggcggcagc catcaacata
    46261 ttgatctgca agccgttgca cttcacctgc gcgtggcgat gccagtcaat gttatagagg
    46321 ctgacaaaca gaccgcaaag gccgagggta atcagccaaa tcgcgttaag cggggagatt
    46381 tgcacatcgt agcttaccag cgacagcgca ccgctcacgc caaccgcgcc agtcagtaca
    46441 gtgaagcccg cggctgccgt atacagacta ccaaccgcgc cgccaattcc ggctatccag
    46501 ccacttagcg ctttttgaaa agagaaatgc cagaacagcg gcggcgacaa accatgccac
    46561 gccgctattg atcagggaaa ttgcgctcat ttagcctctc cactttgagc ctgctgaaac
    46621 aaggtgagat cgccaaagtc cgtgttaaag gtcagctcac gcttacgttt gctgacgcgg
    46681 gcgatatcgg tgttatccac cagatgcagg gctttagtcg ggcacatccg cacacaggcc
    46741 ggaccttgtt catcaaagct acaaaggtca catttgacgg cgatcgcgcg aatacctggc
    46801 acccagtcaa gcaatgtgct gacacgcgcc ggagcaggcg gtgccggtgg cgctttcggg
    46861 gtattggcgt ttgccggaat atccagcgga cggctgccgg aaaattcaat tgcgccaaac
    46921 gggcaggcga tgccgcacag tttgcagctt acgcacagac tttcattcaa ctgcacggcc
    46981 ccatcaacgc gggtgatggc gttaaccggg cagaccaccg cgcagggtgc atcttcacag
    47041 tggtgacaga gctgcggcgc agattctttt tcattgagca tcactctcag gcgcggcatt
    47101 gattgcaggc cgtgctggcg atgcgtctct gaacaggcgg cctcacaggt gtggcagccg
    47161 atacagagcg tggagtcggc aattacaaaa cgattcacca ggcattcctc aggtgattgt
    47221 catttttgac gaaaacatgc cgatgaaatg tcattttcga cactcatcga cacgcccatc
    47281 cccaaacagg cgtaacgcct gcaaaacggg caaagcctca gctcatgctg ccgggctttg
    47341 tccctttacc agttggctta aattcaccgg cacattgttt tcaatggcgg tgtataagct
    47401 gtcgtaaacg ccagcgattt tttcgagata gtgttccaga acctgttcgc gatcgcggat
    47461 gttgaccttg ccatcaatca tgccgtcaat gctcagttgc gcctgcgcaa tccgctgttt
    47521 gtcttcgcca tcttttgttt cgacgtaata ctcgatggtg tggctgttaa agccatccgc
    47581 cagggtgacg taaatacgaa aatgttcaaa aaggacgaaa cagagttctt tgtccggcgc
    47641 gcagctcatg gcgtgatgca ggcgcgcttt cgataacgtg atcccctgaa ccagcgaatt
    47701 gcagtagatg tgccactggt cctgtaggcg acgatgccgc tgtgcgatgt aatcggcttt
    47761 ctcgcttatt tcccaaatag tcattgtcag gttactcgtt taacagagat gccagcttta
    47821 agcatttttt gtgccaactt ttaatttatt gttattaaag agatttttaa gctaaagatg
    47881 aatttcgtcg ccgtgtcgac gtgtcatttc gacatcatcg acattattca ccgcagggat
    47941 aatcaacact ggcgcaatta ttgcttgtag ctggcaatag ttaatgggag gcgatatgca
    48001 cgaaataacc ctctgccaac gggcactgga attgatcgaa cagcaggccg caaaacacgg
    48061 cgcaaaacgc gtaactgggg tctggctcaa aattggcgca ttttcttgtg tcgaaaccag
    48121 ctctcttgcc ttttgttttg atctggtttg ccgcggcagc gtggcggaag gttgtaaact
    48181 gcacctcgaa gaacaagagg ccgaatgctg gtgtgaaaca tgccaacagt atgtgacgct
    48241 actgacccag cgcgtccgcc gctgtccaca gtgtcatggt gacatgctac agattgtggc
    48301 agacgacggt ttacagattc ggcggataga aatagaccag gagtgagcga tgtgtacaac
    48361 atgcggttgc ggtgaaggca acctgtatat cgagggtgat gaacataacc ctcattccgc
    48421 gtttcgtagc gcgccatttg ccccggcggc acgcccgcag atgaaaatca ccggcattaa
    48481 agcgcctgaa tttaccccca gccagactga agaaggcgac ctgcattacg gtcatggcga
    48541 agcgggcact cacacaccgg gcatgagcca gcgtcggatg ctggaagtcg aaattgacgt
    48601 gctggacaaa aacaaccgtc tggctgaacg caaccgcgcg cgctttgctg cccgcaagca
    48661 actggtgctc aacctggttt ccagccctgg ttccggtaaa accaccctgc tgacggaaac
    48721 cttaatgcgc ctgaaagaca gcgttccgtg cgcggttatt gaaggcgacc agcaaaccgt
    48781 gaacgatgcc gcacgcattc gcgctaccgg tacaccagcg attcaggtga acaccggtaa
    48841 aggctgccat cttgacgcac agatgattgc cgacgccgca ccgcgtctgc cactggacga
    48901 taacggtatt ctgtttatcg aaaacgttgg caacctcgta tgcccggcca gcttcgatct
    48961 cggtgaaaaa cacaaagtag cggtgctttc cgttaccgaa ggtgaagaca aaccgctgaa
    49021 atatccgcat atgtttgccg ccgcctcgct gatgctgctc aacaaagttg acctgttgcc
    49081 gtatctcaac tttgacgttg agaagtgcat cgcctgcgcc cgcgaagtca atccagaaat
    49141 tgaaatcatc cttatttccg ccaccagcgg cgaagggatg gaccagtggc tgaactggct
    49201 ggagacacag cgatgtgcat aggcgttccc ggccagatcc gcaccattga cggcaaccag
    49261 gcgaaagtcg acgtctgcgg cattcagcgc gatgtcgatt taacgttagt cggcagctgc
    49321 gatgaaaacg gtcagccgcg cgtgggccag tgggtactgg ttcacgttgg ctttgccatg
    49381 agcgtaatta atgaagccga agcacgcgac actctcgacg ccttacaaaa catgtttgac
    49441 gttgagccgg atgtcggcgc gctgttgtat ggcgaggaaa aataatgcgt tttgttgatg
    49501 aatatcgcgc gccggaacag gtgatgcagt taattgagca tctgcgcgaa cgtgcttcac
    49561 atctctctta caccgccgaa cgccctctgc ggattatgga agtgtgtggc ggtcataccc
    49621 acgccatttt taaattcggc ctcgaccagt tactgcctga aaacgttgag tttatccacg
    49681 gtccgggttg cccggtgtgc gtactgccga tgggtagaat cgacacctgc gtggagattg
    49741 ccagccatcc ggaagtcatc ttctgtacct ttggcgacgc catgcgcgta ccagggaaac
    49801 agggatcgct gttgcaggca aaagcacgcg gtgccgatgt gcgcatcgtt tactcgccga
    49861 tggatgcgtt gaaactggcg caggagaatc caacccgcaa agtggtgttc ttcggcttag
    49921 gttttgaaac caccatgccg accaccgcca tcactctgca acaggcgaaa gcgcgtgatg
    49981 tgcagaattt ttacttcttc tgccagcaca ttacgcttat cccgacgctg cgcagtttgc
    50041 tggaacagcc ggataacggt atcgacgcgt tcctcgcgcc gggccacgtt agtatggtta
    50101 tcggcactga tgcctataat tttatcgcca gcgattttca tcgtccgctg gtggtcgctg
    50161 gtttcgaacc ccttgatcta ctgcaaggcg tggtcatgct ggtggagcag aaaatagcgg
    50221 cccacagcaa ggtagagaat cagtatcgtc gggtggtacc ggatgccggt aacctactgg
    50281 cgcaacaggc gattgccgat gtgttctgtg tcaacggcga cagcgaatgg cgcggcttag
    50341 gcgtgattga atcttctggc gtgcacctga cgccggatta tcaacgattc gatgccgaag
    50401 cacatttccg cccggcaccg cagcaggtct gcgatgaccc gcgcgcgcgt tgtggtgagg
    50461 tattaacggg caaatgtaag ccgcatcaat gcccgctgtt tggtaacacc tgtaatcctc
    50521 aaaccgcatt tggtgcactg atggtctcct ccgaaggagc gtgcgccgcg tggtatcagt
    50581 atcgtcagca ggagagtgaa gcgtgaataa tatccaactc gcccacggca gcggcggcca
    50641 ggcgatgcag caattaatca acagcctgtt tatggaagcc tttgccaacc cgtggctggc
    50701 agagcaggaa gatcaggcac gtcttgatct ggcgcagctg gtagcggaag gcgaccgtct
    50761 ggcgttctcc accgacagtt acgtcattga cccgctgttc tttcctggcg gtaatatcgg
    50821 caagctggcg atttgcggca ccgcgaatga cgttgcgggc agtggcgcta ttccgcgcta
    50881 tctctcctgt ggctttatcc tcgaagaagg attgccgatg gagacactga aagccgtagt
    50941 gaccagcatg gcagaaaccg cccgcacggc aggcattgcc atcgttactg gcgatactaa
    51001 agtggtgcag cgcggcgcgg cagataaact gtttatcaac accgctggca tgggcgcaat
    51061 tccggcgaat attcactggg gcgcacagac gctaaccgca ggcgatgtat tgctggttag
    51121 cggtacactc ggcgaccacg gggcgactat ccttaacctg cgtgagcagc tggggctgga
    51181 tggcgaactg gtcagcgact gcgcggtgct gacgccgctt attcagacgc tgcgtgacat
    51241 tcccggcgtg aaagcgctgc gtgatgccac ccgtggtggt gtaaacgcgg tggttcatga
    51301 gttcgcggca gcctgcggtt gtggtattga aatttctgaa tcagcgctgc cggttaaacc
    51361 tgccgtgcgc ggcgtttgcg aattgctggg actggacgcc ctgaactttg ccaacgaagg
    51421 caaactggtg atcgccgttg aacgcaacgc ggcagagcaa gtgctggcag cgttacattc
    51481 ccatccactg gggaaagacg cggcgctgat tggtgaagtg gtggaacgta aaggtgttcg
    51541 tcttgccggt ctgtatggcg tgaaacgaac cctcgattta ccacacgccg aaccgcttcc
    51601 gcgtatatgc taataaaatt ctaaatctcc tatagttagt caatgacctt ttgcaccgct
    51661 ttgcggtgct ttcctggaag aacaaaatgt catatacacc gatgagtgat ctcggacagc
    51721 aagggttgtt cgacatcact cggacactat tgcagcagcc cgatctggcc tcgctgtgtg
    51781 aggctctttc gcaactggta aagcgttctg cgctcgccga caacgcggct attgtgttgt
    51841 ggcaagcgca gactcaacgt gcgtcttatt acgcgtcgcg tgaaaaagac acccccatta
    51901 aatatgaaga cgaaactgtt ctggcacacg gtccggtacg cagcattttg tcgcgccctg
    51961 atacgctgca ttgcagttac gaagaatttt gtgaaacctg gccgcagctg gccgcaggtg
    52021 ggctataccc caaatttggt cactattgcc tgatgccact ggcggcggga gggcatattt
    52081 ttggtggctg tgaatttatt cgttatgacg agcgtccctg gagcgaaaaa gagttcaatc
    52141 gtctgcaaac atttacgcag atcgtttctg tcgtcaccga acaaatccag agccgcgtcg
    52201 tcaacaatgt cgactatgag ttgttatgcc gggagcgcga taacttccgc atcctggttg
    52261 ccattaccaa cgcggtgctt tcccgcctgg atatggacga actggtcagc gaagtcgcca
    52321 aagaaatcca ttactatttc gacattgacg atatcagcat tgtcttacgc agccaccgta
    52381 aaaacaaact caacatctac tccactcact atcttgataa acaacatccc gcccacgaac
    52441 agagcgaagt cgatgaagcc ggaaccctca ccgaacgcgt gttcaaaagt aaagagatgc
    52501 tgctgatcaa tctctacgag cgggacgatt tagcccccta tgaacgcatg ttgttcgaca
    52561 cctggggcaa ccagattcaa accttgtgcc tgttaccgct gatgtctggc gacaccatgc
    52621 tgggcgtgct gaaactggcg caatgcgaag agaaagtgtt taccactacc aatctgaatt
    52681 tgctgcgcca gattgccgaa cgtgtggcaa tcgctgtcga taacgccctc gcctatcagg
    52741 aaatccatcg tctgaaagaa cggctggttg atgaaaacct cgccctgacc gagcagctca
    52801 acaatgttga tagtgaattt ggcgagatta ttggccgcag cgaagccatg tacagcgtgc
    52861 ttaaacaagt tgaaatggtg gcgcaaagtg acagtaccgt gctgatcctc ggtgaaactg
    52921 gctcgggtaa agagctgatt gcccgtgcga tccataatct cagtgggcgt aataatcgcc
    52981 gcatggtcaa aatgaactgc gcggcgatgc ctgccggatt gctggaaagc gatctgtttg
    53041 gtcatgagcg tggggctttt accggtgcca acgcccagcg cattggtcgt tttgaactgg
    53101 cggataaaag ctctctgttc ctcgacgaag tgggcgatat gccactggag ttacagccga
    53161 agttgctgcg tgtattgcag gaacaggagt ttgaacgtct cggcagcaac aaaatcattc
    53221 agacggacgt acgtttaatt gccgcgacta accgcgatct gaaaaaaatg gtcgccgacc
    53281 gagagttccg tagcgatctc tattaccgcc tgaacgtatt cccgattcac ctgccgccac
    53341 tacgcgagcg tccggaagat attccgctgc tggcgaaagc ctttaccttc aaaattgccc
    53401 gtcgtctggg gcgcaatatc gacagcattc ctgccgagac gctgcgcacc ttgagcaaca
    53461 tggagtggcc gggtaacgta cgcgaactgg aaaacgtcat tgagcgcgca gtattgctaa
    53521 cacgcggcaa cgtgctgcag ctgtcattgc cagatattgc tttaccggag cctgaaacgc
    53581 cgcctgccgc aacggttgtc gcccaggagg gcgaagatga atatcagttg attgtgcgcg
    53641 tgctgaaaga aaccaacagc gtggttgccg ggcctaaagg cgctgcgcaa cgtctggggc
    53701 tgaaacgcac gaccctgctg tcacggatga agcggctggg aattgataaa tcggcattga
    53761 tttaactgca aattgccgga cagatctgcc tgtccggcat actattcacg aggttttttc
    53821 ggacgatatt tttccggcag ttctggcacc ggacgcttgt catcgatgag atgacgcacg
    53881 gttaagatcg gatgacgcca cagcattctc ggcccggccc aacgcataat ctgtttcatc
    53941 tcttcacgct ttgcaggctg gtaacagtgc accggacact gcttacaggc tggtttctct
    54001 tcgccgaaca cacatttatc cagccgcttt tgcgcgtaaa caaacaacgc ctcgtaatgc
    54061 tccggctccg ctgacgcctg cgggcatttc gcttgataaa gatcgatcat ttttttaatc
    54121 gtcagttttt cacgagagat acgcttgccg gacatgctgc ctccacctca ttaagatgca
    54181 tttatattac atcttaatc
//
LOCUS       DAETQL010000020        53215 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.020, whole genome shotgun sequence.
ACCESSION   DAETQL010000020 DAETQL010000000
VERSION     DAETQL010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.020"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(12..323)
                     /locus_tag="K0W65_001265"
     CDS             complement(12..323)
                     /locus_tag="K0W65_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBI3094598.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            486..582
                     /gene="ffs"
                     /locus_tag="K0W65_001266"
     ncRNA           486..582
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="K0W65_001266"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            702..1055
                     /locus_tag="K0W65_001267"
     CDS             702..1055
                     /locus_tag="K0W65_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBI3094599.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="K0W65_001268"
     CDS             complement(1097..2647)
                     /gene="pdeB"
                     /locus_tag="K0W65_001268"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HBI3094600.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2811..3281)
                     /locus_tag="K0W65_001269"
     CDS             complement(2811..3281)
                     /locus_tag="K0W65_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HBI3094601.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3397..3948)
                     /gene="maa"
                     /locus_tag="K0W65_001270"
                     /pseudo
     CDS             complement(3397..3948)
                     /gene="maa"
                     /locus_tag="K0W65_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4120..4338)
                     /gene="hha"
                     /locus_tag="K0W65_001271"
     CDS             complement(4120..4338)
                     /gene="hha"
                     /locus_tag="K0W65_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBI3094602.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="K0W65_001272"
     CDS             complement(4364..4738)
                     /gene="tomB"
                     /locus_tag="K0W65_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBI3094603.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5284..8433)
                     /locus_tag="K0W65_001273"
     CDS             complement(5284..8433)
                     /locus_tag="K0W65_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBI3094604.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="K0W65_001274"
     CDS             complement(8456..9649)
                     /gene="acrA"
                     /locus_tag="K0W65_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBI3094605.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9791..10438
                     /gene="acrR"
                     /locus_tag="K0W65_001275"
     CDS             9791..10438
                     /gene="acrR"
                     /locus_tag="K0W65_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBI3094606.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10566..13928
                     /gene="mscK"
                     /locus_tag="K0W65_001276"
     CDS             10566..13928
                     /gene="mscK"
                     /locus_tag="K0W65_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBI3094607.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="K0W65_001277"
     CDS             complement(14140..14301)
                     /gene="rsmS"
                     /locus_tag="K0W65_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBI3094608.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14315..14842)
                     /gene="priC"
                     /locus_tag="K0W65_001278"
     CDS             complement(14315..14842)
                     /gene="priC"
                     /locus_tag="K0W65_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBI3094609.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14912..15289
                     /locus_tag="K0W65_001279"
     CDS             14912..15289
                     /locus_tag="K0W65_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBI3094610.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15442..15993
                     /gene="apt"
                     /locus_tag="K0W65_001280"
     CDS             15442..15993
                     /gene="apt"
                     /locus_tag="K0W65_001280"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBI3094611.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16122..18047
                     /gene="dnaX"
                     /locus_tag="K0W65_001281"
     CDS             16122..18047
                     /gene="dnaX"
                     /locus_tag="K0W65_001281"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBI3094612.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17380..17444
                     /gene="dnaX"
                     /locus_tag="K0W65_001281"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18100..18429
                     /locus_tag="K0W65_001282"
     CDS             18100..18429
                     /locus_tag="K0W65_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBI3094613.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18429..19034
                     /gene="recR"
                     /locus_tag="K0W65_001283"
     CDS             18429..19034
                     /gene="recR"
                     /locus_tag="K0W65_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBI3094614.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19144..21018
                     /gene="htpG"
                     /locus_tag="K0W65_001284"
     CDS             19144..21018
                     /gene="htpG"
                     /locus_tag="K0W65_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBI3094615.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21199..21843
                     /gene="adk"
                     /locus_tag="K0W65_001285"
     CDS             21199..21843
                     /gene="adk"
                     /locus_tag="K0W65_001285"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBI3094616.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21975..22937
                     /gene="hemH"
                     /locus_tag="K0W65_001286"
     CDS             21975..22937
                     /gene="hemH"
                     /locus_tag="K0W65_001286"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBI3094617.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22934..23893)
                     /gene="aes"
                     /locus_tag="K0W65_001287"
                     /pseudo
     CDS             complement(22934..23893)
                     /gene="aes"
                     /locus_tag="K0W65_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24045..25349
                     /gene="gsk"
                     /locus_tag="K0W65_001288"
     CDS             24045..25349
                     /gene="gsk"
                     /locus_tag="K0W65_001288"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBI3094618.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25482..27158)
                     /locus_tag="K0W65_001289"
     CDS             complement(25482..27158)
                     /locus_tag="K0W65_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBI3094619.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27396..28616)
                     /gene="fsr"
                     /locus_tag="K0W65_001290"
     CDS             complement(27396..28616)
                     /gene="fsr"
                     /locus_tag="K0W65_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HBI3094620.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28834..30486
                     /gene="ushA"
                     /locus_tag="K0W65_001291"
     CDS             28834..30486
                     /gene="ushA"
                     /locus_tag="K0W65_001291"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBI3094621.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="K0W65_001292"
     CDS             complement(30523..31002)
                     /gene="ybaK"
                     /locus_tag="K0W65_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBI3094622.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31206..32000)
                     /locus_tag="K0W65_001293"
     CDS             complement(31206..32000)
                     /locus_tag="K0W65_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HBI3094623.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32138..32479
                     /locus_tag="K0W65_001294"
     CDS             32138..32479
                     /locus_tag="K0W65_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBI3094624.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32694..35198)
                     /gene="copA"
                     /locus_tag="K0W65_001295"
     CDS             complement(32694..35198)
                     /gene="copA"
                     /locus_tag="K0W65_001295"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBI3094625.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35460..36392
                     /gene="glsA"
                     /locus_tag="K0W65_001296"
     CDS             35460..36392
                     /gene="glsA"
                     /locus_tag="K0W65_001296"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBI3094626.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36395..37687
                     /locus_tag="K0W65_001297"
     CDS             36395..37687
                     /locus_tag="K0W65_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI3094627.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37812..38219
                     /gene="cueR"
                     /locus_tag="K0W65_001298"
     CDS             37812..38219
                     /gene="cueR"
                     /locus_tag="K0W65_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HBI3094628.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38220..38678)
                     /locus_tag="K0W65_001299"
     CDS             complement(38220..38678)
                     /locus_tag="K0W65_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBI3094629.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38675..39592)
                     /locus_tag="K0W65_001300"
     CDS             complement(38675..39592)
                     /locus_tag="K0W65_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBI3094630.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39738..40415
                     /gene="fetA"
                     /locus_tag="K0W65_001301"
     CDS             39738..40415
                     /gene="fetA"
                     /locus_tag="K0W65_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBI3094631.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40402..41181
                     /gene="fetB"
                     /locus_tag="K0W65_001302"
     CDS             40402..41181
                     /gene="fetB"
                     /locus_tag="K0W65_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBI3094632.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="K0W65_001303"
     CDS             complement(41244..42098)
                     /gene="cnoX"
                     /locus_tag="K0W65_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBI3094633.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="K0W65_001304"
     CDS             complement(42159..42968)
                     /gene="ybbO"
                     /locus_tag="K0W65_001304"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBI3094634.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="K0W65_001305"
                     /pseudo
     CDS             complement(42958..43580)
                     /gene="tesA"
                     /locus_tag="K0W65_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43551..44237
                     /locus_tag="K0W65_001306"
     CDS             43551..44237
                     /locus_tag="K0W65_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3094635.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44234..46649
                     /locus_tag="K0W65_001307"
                     /pseudo
     CDS             44234..46649
                     /locus_tag="K0W65_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47362..48456)
                     /gene="mnmH"
                     /locus_tag="K0W65_001308"
     CDS             complement(47362..48456)
                     /gene="mnmH"
                     /locus_tag="K0W65_001308"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBI3094636.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48525..49451)
                     /gene="allS"
                     /locus_tag="K0W65_001309"
     CDS             complement(48525..49451)
                     /gene="allS"
                     /locus_tag="K0W65_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HBI3094637.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49681..50163
                     /gene="allA"
                     /locus_tag="K0W65_001310"
     CDS             49681..50163
                     /gene="allA"
                     /locus_tag="K0W65_001310"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HBI3094638.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50241..51056
                     /gene="allR"
                     /locus_tag="K0W65_001311"
     CDS             50241..51056
                     /gene="allR"
                     /locus_tag="K0W65_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HBI3094639.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51146..52927
                     /gene="gcl"
                     /locus_tag="K0W65_001312"
     CDS             51146..52927
                     /gene="gcl"
                     /locus_tag="K0W65_001312"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HBI3094640.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52940..>53215
                     /locus_tag="K0W65_001313"
     CDS             52940..>53215
                     /locus_tag="K0W65_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBI3094641.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIR"
BASE COUNT        12830 a        13693 c        14018 g        12674 t
ORIGIN      
        1 cccggagagg gttagtagtt ccagcgataa cgctgcaagt cgatttgccc gcttcccgat
       61 accatcacac cttctgccag taatgcctgt cgctgacgct gtaaatccgg cccggttagc
      121 gaaattgtgc catggcgatt aaccacccgg tgccagggta aggtgctgcc ttcagggaga
      181 cgctttaaca caccgcccac ctggcgcgcg gcgcggggcg atcccgccag tttcgccaca
      241 tcaccgtaag tggtgacata gccttcggga atagcggcga cgatttgcca gacgcgttgg
      301 ggaaatgaat cttctttttc catcttttct tcctgaggta atttttcagc ataatctgga
      361 aaaacgcccg agtgaagtcg cattgcgcaa gaaaccagca tctggcacgc aatgggttgc
      421 aattagccgg ggcagcagtg ataatgcgcc tgcgcgttgg ttttcaacgc tctcaatggg
      481 ggctctgttg gttctcccgc aatgctactc tgtttaccag gtcaggtccg gaaggaagca
      541 gccaaggcag atgacgcgtg tgccgggatg tagctggcag ggcccccacc catttctgcc
      601 tcccaccgtt tcgtcaaaaa atcccagcat ggctaaactt taaccacaac tgacgtcgca
      661 agaattgtct ggctgcgcag tacgcttcgg aggtatgtct gatgaagtat gttgatggtt
      721 ttgtggttgc cgttcctgcc gataaaaagg atgcctatcg ggaaatggcc gctaaggccg
      781 cgccattgtt taaagagttt ggcgcgcttc gtattgtcga atgctgggcc agcgatgtgc
      841 cggatggcaa agtgaccgat tttcgtatgg cggtgaaagc ggaagagaat gaagaggttg
      901 tctttagctg gattgaatac ccttcaaaag aggtccgcga cgcagctaat caaaagatga
      961 tgtcggaccc acggatgaaa gagttcggcg agtccatgcc gtttgacggc aagcgaatga
     1021 tctatggcgg attcgagtca atcatcgacg aataggtagc gtgacgggct gtgcggcgtg
     1081 ccgtcagccc aaaaattcac aaatgttgct cggcccagcg taagaaatct tctttcggta
     1141 atgccttgct gtagagccag ccctgaccgt agtgcacgcc atgctggcgt agccactctt
     1201 cttgtttact ggtttcgatt ccctccgcta ccattttcag tttcagtgtt tttgccattt
     1261 cgatgatatg cggcgtgaca tttttatatt ccagcgcatc aacgaaagat ttatcgatct
     1321 tcagaatgtc gacatccaga tcctgtaaat aacttaaact tgaatacccc gtaccaaaat
     1381 catcaagata aatttcatgg cccgcctccc ggtagcgaga aattatcggg gcgctggttt
     1441 tcggatcggc aaactcgcgt tcagtgagtt caagcgcgat ctgtctggga ttaacctgat
     1501 agtgattgat catttcacgc agcaattgcg ggattttttc cgaggtgagc acggtggatt
     1561 caagattgat cgaaatatgc tgctgtggat gctgacgcaa gcagtcgccc atatcttcaa
     1621 agacgcttct tataatcagt agcgtcaatg gctcagaaag gcccgtttgc tgtgccagcg
     1681 gaataaaact atctggtgac aaccaactac cgtctgtctg cggccagcgc gccagtgcct
     1741 cagcaccgac aattttgcca ttggctaagg agacaatcgg ctgatagtgc acgcaaatat
     1801 cacgattttc gatagcatcc tgcagccgat gatgcggtga ttggatacgg cgcaaaatac
     1861 gcagcacaaa catcgctgcc agcaggccaa tcaccaaccc gagcggtaac caaataaaga
     1921 cttgccgatg ccagcctttc tgcaacattt tcgttgaagc ccaggtgatg atcgaaatat
     1981 tcatctccag gaagggcagt atatcgtaaa tgatcccatt attttcgata tgctcaccgg
     2041 gtgttttttg tagcctggta ataattccct gagcaatttc atcgctgctg gttatgacaa
     2101 cgttatgggc attgccaata atggcggcat caatttgcca tgaactatag ggaatgacat
     2161 caataaagga agcggggtcg atcatgacga cataatgtgc cgttcccatg gcgaccatgt
     2221 aacggataat gcctaaatcg ttatgcgatg ttaaccagac acgataacca tctttcgaaa
     2281 ttttacctag ctcgggaaag gtatcgggcg gactttcatg ctccagagac gaacactggg
     2341 gaacgttgtt atcgatataa accacttcct gaatatagcg ataactgtaa gatacccgac
     2401 gcatttccat gagatgggct tcgctacagg cagcgccttg ccatctttcc agctcctgca
     2461 gcgcatcttt cccttgtgtc gccaccttat tggctcgaat agcgacgcgg gaggaatagg
     2521 tatccagctc ttcaataaac gatgtttcta cctgctgatg ggccagccag atgcttaagc
     2581 cgacaggcag caatactgaa agaatcagta ctcccgaaat aaggccgacc agatgtcgtg
     2641 ttctcaccag catatcctta ttaacacagt gtagggaaag tatatctgat tgtggaaaaa
     2701 ggctggcagg agataaggag gtagagctgg tatttcatga agtgatgggt ggaaacgatc
     2761 attgtcattt gaccaggcgc agagctttac gcctggtatg taaccctaac ttaagatgtt
     2821 gattcggcgc gagcgagggt aaaaatatcg taaaaagaca gttcaccttt acggacgatc
     2881 attttttgca gttgttcctt atgaacatcc gcgacacgcg gagagttcaa gatggcttca
     2941 accagcgcgg caggtacaaa gcgcgtgttg taccattcgc cgttatagca aacgcggaaa
     3001 tccagcacgt cgatatcttc atagcggtag cgtgggtaga catcgaaaaa gaagaaacca
     3061 ttaagcagga taaataatac aaccagtagc cagacagagc ctgacagcgt ttcggactgc
     3121 aacatcaccg ccagggtggc aaaaaaagca acgtacatac cgataaacag ccccggatgt
     3181 ttacggataa aactgatact aaagcggggt ttgttgtcgc gtttttcgcg ggtattcaga
     3241 gactcaatcg tttcggtcag caggcgttgt atttcggtca tcgtttgcct tcgtggtatc
     3301 tgcaactttc cagggaacac cctattttaa cgtgggaata gtagagggga agtaacagat
     3361 ctaccacaat tttgcataac agttgcgaaa aaccgattac aattttttaa ttattctggc
     3421 tggattaccg cccacgacaa cgttgtccgg gacacctttt gtgacaactg cacctgaagc
     3481 taccacgacg ttatcaccaa tggtcacacc agggttaatg accgcgcgtc cgccaatcca
     3541 gacgttatta ccgatggtga cgggtttccc cagttcagca ccgctattac gtgctacagg
     3601 gtcgatggga tgtgttgccg tgtagatatg aacgcctggt gccaacatac agttatcacc
     3661 gatgcgaata gggcagacat caagcatcac gcaatcgaag ttggcgaaaa aattattgcc
     3721 gagaaaaatg ttatagccat aatcacagcg aaacgttggc tcaatataag cctctgtcac
     3781 ctgaccgaat aaattagcga gaatttactg gcgtaatgtg tgctcttccg ccagggaatg
     3841 attgtatcgg tgaataagct gacgagcgcg caggcgatcg cgagataacg tctcatctgc
     3901 cgagcgatac aactcaccag caatcatctt ttctttttct gtgctcatta ctgacctccg
     3961 gttgctgaaa atgcggcaac acagtaagac gaaaagtcag tgaagagaac gttctcatta
     4021 catctgtgtg ataaagatca catagggtat gtcttcatgg cgaaaaagta taaaattctt
     4081 aataaacagc cggttatagc accgaaagcg aattatttat tagcgaataa atttccatac
     4141 tgaggaaggg atcttgtcgt acagtttatt catggtcaat tcggcgaggc ggtgatctgc
     4201 ggctgagtaa aataccgcca gttcattatc tgataattcg tatttatttt tctcgataac
     4261 acgctccagc gtgtcaattg tctggcaacg acgtaaacgc attaaataat cggtttttgt
     4321 taaaggtttt tcggacatac ttctacccat ggttgtaata attctaacaa gataaactcg
     4381 caggattctc tttcgtcgcg ttgacaaaac aacggaatag tcgattacct gacttccgcc
     4441 atttctgaag agcctgcata ttaataccat aactgctgaa caacataaag gtgtcatcca
     4501 gatattcgtc gatctgctca atgagcttat tgtcttcatt atacttaatt ttgtaattaa
     4561 gtgcgaaggt cgcaatatgc tcaatcagtt cattcaactg gaggttgatc gccgaggttg
     4621 ggtcgttaac ccaaccatga ttgctttctt caaggtttgc aagacagtca tgatacaggg
     4681 tttcacagag aaacttaagc tgtgcgatat catgtctttt gggtgagtat tcatccataa
     4741 cgcgtcccct tcttagtggt tgaactaacg gacacctttc gggatggaaa aaacttactg
     4801 acctagactt gccctctcgt ttgttagctt aactataagc cactctttgc aggttttcat
     4861 cgcattatta cgaaacatta caattaggaa ggaatcggga caggactcac tttttcatac
     4921 tagcactaac ccgcagcagg taaaagcagt ttgctcgaac tgttcataac atttcaggat
     4981 tatcgccaga tgaaattggt gacactaata ccaggattgc tctgaatatg acgtaataac
     5041 cgaggaatga ataaagaatt accgcaaata attaagaata gcctctaaat gattatggat
     5101 tattaaatct attagcgcta cttaatataa attaactaac aatcagtatt ttttatgaat
     5161 tttatacgtg gttaatactg gttttcgtat gagatcctga gttggtggtt caattactcc
     5221 ttaatgttcg taggttatgc ataaaaaagg ccgcttacgc ggccttagtg attacatgtt
     5281 gtatcaatga tgatcgacag tatggctgtg ctcgatatct tcattcttgc ggctaaagcg
     5341 gcggcgaacc accacaaaga ataccggaac gaagaagatt gccagtaccg ttgcggtcac
     5401 catcccgccc attacaccgg tacctactgc gttctgcgcg ccggaaccag caccagtact
     5461 gataaccagc ggcataacgc cgaggataaa cgccagcgag gtcatcagga tcggacgtaa
     5521 acgcatccgc accgcatcaa gcgtcgcttc aatcagacct ttaccttctt tatccatcaa
     5581 gtctttggcg aattcgacga taagtatcgc gttcttcgcc gacaacccaa tggttgtgag
     5641 cagtcctact tggaagtaaa cgtcattggt caggccacgg aaggtggcag ccagcaacgc
     5701 accgataacc cccagcggaa cgaccagcat aacggagaac ggagtcgacc agctctcgta
     5761 cagcgccgcc agacacagga atacgacaat caacgaaatc gcgtacagtg aaggtgcctg
     5821 gttgccggag agacgttcct gataggacat ccccgtccag tcatagccaa caccggtagg
     5881 cagtttgctc gccagttgtt ccatcagctc cattgcttca ccggtacttt tacccggtgc
     5941 cgcctggcct aagatttcca tggatggcag gccgttgtaa cgttccagac gcggcgaacc
     6001 gtactcccaa cgagaagagg agaacgccga gaatggcacc atctgaccat cagcagcacg
     6061 aacataccag tcgccgatat catccggcag catacggtat ttcgcttctg acatgacgta
     6121 aactttcttc acacgaccgc ggtcgataaa gtcgttcaca tagctaccgc cccatgcagc
     6181 gcccagagtg gtgttaatgt cgttgataga aacacccagc gcctgcgctt tttcctggtc
     6241 gatatcaatc ttaaactgcg gggtatcttc cagaccgttt ggacgtacgc tggtcagcat
     6301 atcagggtgc ttcgctgctt ctgcaagcag ctggttacgc gcctgagtca gtttttcgtg
     6361 accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg gttgcagtac ccagttccac
     6421 gattgcgggc aggttaaagg cgaaaaccat cgcatctttg atttgcgaga aagcgcgtgt
     6481 tgcacgcatg gtaatcgctt caactttgtt ttcttcgccc ggacgatcgg cccagtcctt
     6541 caaggaaacg aacgcaatac cggtattctg accacgtccc gcaaagccga agccgttaac
     6601 ggcgaacacc gactcaacgt tgttcttttc tttggtcaga tagtaatgcg ttacctcatt
     6661 gagcactttc tgcgtacgtt cctgcgttgc acctgctggc agctgaacca tggtcataaa
     6721 tacgccctgg tcctcatctg gcaagaagga gcttggcaga cgcacgaaca gataggccat
     6781 gccgaccacg atgatcagat acagcactag gtaacgcccc gtactgcgca gaataccgcc
     6841 tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg cggttcaacc agccgaagaa
     6901 gcctttttta ccttccccgt gatcgccttt ggcaatcggt ttcagcatgg tggcacaaag
     6961 agccggagtc aggatcaacg ctaccagtac cgacagcgcc attgctgaaa caatggtaat
     7021 agagaactga cgatagatag caccagtaga accgccaaag aaggccatcg gtacgaatac
     7081 cgccgacagt accatcgcga taccgaccag agcgccctga atctgcccca tcgacttacg
     7141 ggtagcttct tttggcggca aaccttcttc cgccataaca cgctccacgt tttctaccac
     7201 aacgatagcg tcatccacca acaagccgat ggcgagcacc atcccgaaca ttgttagcgt
     7261 gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc ccgagcaata ccaccggtac
     7321 ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcagg aacagataca taaccaggaa
     7381 cacgaggatg atcgcttcga ccagcgtttt aaccacttcg tgaatagaga ttttcacgaa
     7441 cggcgtggtg tcgtacgggt aaacaatttt cagacccgac gggaagaacg gttccatctt
     7501 cgccagttca gcacggattg ccgcagcggt atccagcgcg tttgcaccgg tcgccagctt
     7561 gatccccaga ccggaagcag gttggccgtt aaactctgcg atgatgtcgt agttctcacc
     7621 acccagctca atcttcgcca catcacgcag cagtacgcgg gaaccatcct gattcacttt
     7681 cagcaggatt ttgccgaact cttcagtaga ggtcagacgc gtctgagcaa taatagaggc
     7741 gttaagctgt tggcctttca ccggcggcgt accaccgagc tgacccgccg caacctgggc
     7801 gttctgcgct ttgatggcgg taatgacatc aaccggcgtt agctggaatt tgttcagctc
     7861 attcgggttc atccagatac gcatcgcgta ctgtgaaccg aacaactgaa catcacccac
     7921 gcccgacgta cggctgatgg catctttcat attcgccgct acgtagtcag agatatcctc
     7981 ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc atcaggaagc tgctggatga
     8041 tttctcaacg ctcacccctt gctgctgaac ttcttgcggc agcaacggca tcgccagctg
     8101 cagtttgttc tgcacctgaa cctgcgcgat atccgcatca gtaccagact caaaggtcag
     8161 ggtgatctgc acggtacccg tggagtcact gttagaggac atgtacatca ggttatcgat
     8221 accgttcata ttctgttcga taacctgtgt caccgtgtcc tgcactgttt tcgcatcagc
     8281 gccggggtag gaggcggaga tcgttactgc cggcggtgca atcgtaggat attgcgccac
     8341 cggcagtttg aggatcgcca gcccccctgc caacatgatg ataatggcga tcacccacgc
     8401 aaaaatcggg cgatcgataa agaaattagg catgtcttaa cggctcctgt ttaagttaag
     8461 acttggactg ttcaggctga gcaccgcttg cggcttgctg gttattatca gcggtaactt
     8521 cttgtgcttt tacctggaca ccaggacgca ctttctgcag cccacttatt actacgcgat
     8581 cgcctgcttt cagaccttct gtcaccagcc acttatcgcc aatagcctgg cttgcaacga
     8641 tcggacgggt ttccactttg tcatccgcgc caactaccag tacggtggca tcaccacgcg
     8701 gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt tggattaagc ccttcttcca
     8761 gacgtgcgcg cacgaacata cccggcagca gagtgtgatc cgggttcggg aagatagcgc
     8821 gtagggtgat agacccagtg gtctgatcaa cggtaacgtc agagaattcc agcgtaccgt
     8881 cctgcgggaa cttaatgccg tcactggtga tcagcgacac tttggctttg ccgttctctt
     8941 gtttcagcgt gccattcgcc agttcctgtt tcaggcgaag gaagtcgttg ctggactggg
     9001 tcacatcaac gtagatcgga tcaagttgct gcacggttgc cagcacagtc gcctgaccgt
     9061 tctgtaccaa tgcgccttcc gtcacgttcg acttaccaat gcgaccgcta atcggagagg
     9121 tgactttggt gtaagccaga ttgatccgcg cagtttcaac ggcagctttc gccgcagtta
     9181 ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc gtacccttgc ttactgatgt
     9241 actgagtacc gagcagtttc tgataacgat tcaccgtcaa ttgcgcgata ttggctgcag
     9301 cctgggcttt cgccagatca cctttcgcac tgtcgtatgt cgcctgatag gtcgcaggat
     9361 caatctgata gagagagaca ccagcttcga tgtcgctacc ttctttgaaa ttacgcttca
     9421 ggataatccc gctaacttga ggacgaactt ctgcgatccg gtaggcactg gtgcgacctg
     9481 gaagctcggt tgtgatctgc agaggttcag ttttgactgt tactacgcca acggcgggca
     9541 tctgctggcc accttgttgg gcctgtttgt cgtcacatcc tgttagggct aagctgcctg
     9601 agagcatcag aacgaccgcc agaggcgtaa accctctgtt tttgttcata tgtaaacctc
     9661 gagtgtccga tttcaaattg gtcaatggtc aaaagttaat aaacccattg ctgcgtttat
     9721 attatcgtcg tgctatggta catacattca caaatgtatg taaatctaac gcctgtaaat
     9781 tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa gaaacgcgcc aacacatcct
     9841 cgatgtggct ctacgtcttt tctcacagca gggggtatca tccacctcgc tgggcgagat
     9901 tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg cattttaaag acaagtcgga
     9961 tttgttcagt gagatctggg aactgtcaga atccaatatt ggtgaactag agcttgagta
    10021 tcaggcaaaa ttccctggcg atccactctc agtattaaga gagatattaa ttcatgttct
    10081 tgaatccacg gtgacagaag aacgacgtcg attattgatg gagattatat tccacaaatg
    10141 cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa cgtaatctct gtctggaaag
    10201 ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa gcgaaaatgt tgcctgcgga
    10261 tttaatgacg cgtcgggcag caattattat gcgcggctat atttccggcc tgatggaaaa
    10321 ctggctcttt gccccgcaat cttttgatct taaaaaagaa gcccgcgatt acgtcgccat
    10381 cttactggag atgtatctcc tgtgccccac gcttcgtaat cctgccacta acgaataacc
    10441 ctgaatctga ctccaggatt tttcctggac attttcgtcg ttgctattct ggttcactgc
    10501 gtcgtgatat tcttgcggtt tgactttttc aggtcgttct tcaggttcag aaaccttcat
    10561 tcatcatgac tatgttccag tattacaaac gatcacggca ttttgttttt tcagcattta
    10621 ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt tgcgcgggcg tcatcgaatg
    10681 gtgatctgcc gacaaaagcg gacctgcagg cgcaacttga ctcactaaat aaacaaaaag
    10741 atctttctgc tcaggacaaa ctggtgcagc aggatctgac agatacatta gccaccctcg
    10801 ataaaatcga tcgcataaaa gaagagacag ttcagctacg gcaaaaagtc gctgaagcgc
    10861 cggaaaaaat gcgccaggcg accgcggcgt taacagcact tagcgatgtc gataacgacg
    10921 aagaaacgcg caaaattctg agtacgcttt cgttgcgcca gctggaaact cgcgttgccc
    10981 aggcgctgga cgatttgcaa aacgcacaaa acgatctggc gtcttataac agccagctgg
    11041 tttcgttaca gacgcagccc gaacgcgtgc aaaatgcgat gtataacgct tcgcagcagc
    11101 tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg cgagactgcc ttacgtccca
    11161 gccagaaagt gttaatgcag gctcagcagg cgttgctgaa tgcggagatt gaccagcagc
    11221 gtaaaagcct ggaagggaac accgtcttgc aggatacctt gcaaaagcaa cgtgattacg
    11281 tgacggcgaa cagcgcccgt ctggagcacc agttgcagct gttgcaagaa gcggtaaaca
    11341 gcaagcgcct gactttaact gaaaaaacgg cgcaggaagc cgtctccccg gatgaagccg
    11401 cgcgtattca ggctaatccg ctggtgaaac aggaactgga aattaaccag cagttaagtc
    11461 agcgtctgat taccgcgact gaaaacggta atcagttgat gcagcaaaac attaaagtca
    11521 aaaactggct ggagcgggcg ctgcaatcgg aacgcaatat taaagagcag attgccgtcc
    11581 tgaagggcag cctgctgttg tctcgtatcc tttaccagca acaacaaacg ctgccctcgg
    11641 cggatgaact ggaaaacatg accaaccgca tcgcggattt gcgtctcgaa cagtttgaag
    11701 ttaaccagca gcgtgatgca ctcttccaga gcgatgcgtt cgtcaacaaa ctggaagaag
    11761 gtcacaccaa cgaagtcaac agcgaagttc acgatgcgtt attgcaagtg gttgatatgc
    11821 gtcgcgaatt gctggatcaa ctcaacaaac agttgggtaa ccagctgatg atggccatta
    11881 acctgcaaat caaccagcag cagttaatga gcgtatcgaa aaacctgaaa tccatcctga
    11941 ctcagcaaat cttttgggtg aacagtaacc gtccaatgga ctgggactgg atcaaagcgt
    12001 tcccgcaaag cctgaaagat gaatttaagt cgatgaaaat cacggtgaac tgggaaaaag
    12061 cctggcctgc cgtttttatc gctttcctcg ccggtttgcc gctgctgttg attgccgggc
    12121 tgatccactg gcgtctgggc tggctgaaag cgtatcaaca gaaactggct tccgctgtgg
    12181 gttccctgcg taacgatagc cagctcaaca caccaaaagc gatccttatc gacctgatcc
    12241 gcgcgctgcc ggtgtgcctg attattctcg cggttggcct gattctgttg accatgcagc
    12301 tcaacatcag cgaactgcta tggtcgttca gtaaaaaact ggcgattttc tggctggtgt
    12361 ttggcctgtg ctggaaggta ctggagaaaa atggcgttgc cgtgcgtcac ttcggcatgc
    12421 cggagcagca gaccagccac tggcgtcggc aaattgtccg catcagtctc gcgttgctgc
    12481 ctatccattt ctggtctgtg gtggcagaac tttccccgct gcatctgatg gatgatgtgc
    12541 tggggcaggc gatgattttc ttcaacctgc tgctgattgc cttcctggta tggccaatgt
    12601 gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg actagtcacc attaccgtgc
    12661 tgtcgattat cccgattgca ttgatggtgc tgactgccac gggttatttc tacaccacgc
    12721 tgcgcctctc cgggcgttgg attgaaaccg tttatctggt gatcatctgg aacttgctct
    12781 accagacggt attgcgcggc ttaagcgtag cggcgcggcg tatcgcctgg cgtcgtgcgc
    12841 tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg tgccgaaccg ccggaagaac
    12901 ccaccattgc actggagcaa gttaaccagc agacgctgcg tattaccatg ttgctgatgt
    12961 ttgcgctgtt cggtgtcatg ttctgggcaa tttggtccga tttgatcacc gtgttcagct
    13021 atctcgacag catcacgctc tggcattaca acggcactga agctggcgct gcggtggtga
    13081 aaaacgtcac catgggcagt ctgttgtttg cgattatcgc ctcaatggtg gcctgggcgt
    13141 tgattcgcaa cctgcctggt ttactggaag tgctggtgct ctcgcgactg aatatgcgcc
    13201 agggcgcgtc gtatgccatt actaccatcc ttaactacat cattattgcc gttggtgcga
    13261 tgacggtgtt cggatcgctg ggcgtctctt gggataaact ccagtggctg gccgcagcat
    13321 tatccgtagg tcttggtttt ggtttacaag aaattttcgg taacttcgtc tccggtttga
    13381 tcattctgtt cgaacgtccg gtgcgtattg gcgatacggt aaccattggt agcttctcgg
    13441 ggacggtaag taagatccgt attcgtgcga caacgattac cgatttcgat cgcaaagaag
    13501 tgatcatccc gaacaaagcg tttgttaccg agcgtctgat caactggtcg ttgactgaca
    13561 ctactacgcg tctggtgatc cgtctcggcg tggcctatgg ctccgatctg gaaaaagtgc
    13621 gtaaagtgtt actgaaggcg gcgactgagc acccaagggt gatgcacgaa ccaatgccgg
    13681 aagtcttctt tacggcattt ggtgccagca cgttggatca tgagctgcgt ctgtatgtgc
    13741 gtgaactgcg tgaccgtagc cgtactgtcg atgagctgaa ccgtactatc gatcagctgt
    13801 gccgtgaaaa cgacatcaac attgccttta accagcttga agtgcatctg cacaacgaga
    13861 agggcgatga ggtgacggaa gtaaaacgcg actacaaagg cgacgatccg acaccagcgg
    13921 ttgggtaata accaaagggg gcgacattta gttgccccga gattactgac aaagtgcgcg
    13981 ttgttcatgc cggatgcggc gtgaccgcct tatccggcct acgaaaccgc aagaattcaa
    14041 tatattgcag gagcggtgta ggcctgataa gcgtagcgca tcaggcagtt ttgcgtttgc
    14101 ccgcaatctt aggggacatt tagcggcccc atttatttct cacttttccg cctcatcatc
    14161 gtgcgttaat ttcttttcat aatcacgctt tacaatatcc agcgcgcgca gcacggtact
    14221 ggcagggatc tgattttcct ccagcagcac aatcaaatcg acagccagtt tgacatcgtc
    14281 aggggcattt tcaagtgaca tactctttcc attgctagcg ggttaagcgc gctagcctgt
    14341 tttcgatttt ttccagcgca tggcggcagc gtgccaggcg cgcttcatag gcttccactt
    14401 cacgatgcag cgtttgctgt tccacgagat cggtcacccg cgccaggcgg gctctgcgtt
    14461 cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg ctgaatacgt ttacgctgcc
    14521 agcgggaaat tttcggtggc gcactgtccc actcgcgcaa tgaccaggcg gaggcttcac
    14581 gcgcgatggc ttccagttgt gccgccagat gttccgccag ccaggccact tgcggcagct
    14641 gttgctgctc aactgcatga cgaagcgcag ccagattatc gcccgcctcg tcgagacaag
    14701 actgtagtgt tgtcgcacga gtctgaaaaa gatgcctgtc gaaacgagca cttagcgtgg
    14761 cgaactgtga caccggggca caacgctgac gcagcgtagc gagctgtcct tccagttttt
    14821 ccagcagcag ggcggttttc acgataagac ctcaatgaaa atgataattg ttatgctaaa
    14881 gtagccacta tgtaagctga caacactcaa tatgcaacga atcattttaa tcatcattgg
    14941 ctggctggcg gtagtgctgg gtacgctggg cgtggtatta ccggtattac cgacgacgcc
    15001 gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc ccgcgctttc acgcctggtt
    15061 gctgtaccgc tcatggtttg gcagctatct acgtttctgg cagaaacatc atgcgatgcc
    15121 gcgcggcgtc aaaccgcggg cgattttgct tattttgctc acgtttgcca tttctctgtg
    15181 gttcgtccag atgccatggg tgcgcatcat gttgctggta attctcgcct gtttgctttt
    15241 ctatatgtgg cgaattccgg tgattgatga aaagcaagaa aagcactgaa gcacaacaat
    15301 cgcagttgca attattgcgt acagccagta cattctggcg ttttcgagca caggcgcagg
    15361 cggtcaaagg ttaaacaaca gttacttttg atacgtttaa aacgcgccgt gagtaccacc
    15421 gtaacaagca ggcatacact tatgaccgcg actgcacagc agcttgagta tctcaaaaat
    15481 agcatcaaaa gcattcagga ctacccaaaa cccggcattc ttttccgcga tgtcaccagc
    15541 ttactggaag acccgaaagc ttacgctctc agcatcgact tgctggttga gcgttacaaa
    15601 aatgcgggca ttaccaaagt tgtcggcacc gaagcgcgtg gcttcttgtt tggcgctccg
    15661 gtagctctgg gtctgggcgt tggctttgta ccggtccgta aaccgggcaa actgccgcgt
    15721 gaaaccatca gtgaaaatta cgacctggaa tacggcaccg atcagctgga aatccacgtt
    15781 gatgccatca aaccgggcga caaagttctg gtggtggacg atctgctggc aaccggcggc
    15841 actatcgaag cgaccgttaa actgatccgt cgtctgggtg gtgaagtggc tgacgctgcg
    15901 ttcattatca acctgtttga tctcggcggc gaacagcgtc tcgaaaaaca gggcattacc
    15961 agctacagcc ttgtcccgtt cccgggccat taattatcgc cagtcttgtg ctgcccacgc
    16021 tacggacagc acaagatgtg cattcagcct cgccgttctg acggggctgt gttagcatta
    16081 ccccttcgtg aatccacctt ccagcgtttc agagcctgcc aatgagttat caggttttag
    16141 cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg ccaggaacat gtgctgaccg
    16201 cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc ttatcttttt tccggcaccc
    16261 gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa ggggctaaac tgcgaaaccg
    16321 gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg tgaaatcgag caggggcgct
    16381 ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa agttgaagat acccgcgacc
    16441 tgctggataa cgtccagtac gctccggcgc gtggtcgttt caaagtttat ctgatcgacg
    16501 aagtgcatat gctgtcgcgc cacagcttta acgcactgtt aaaaaccctt gaagagccgc
    16561 cggagcacgt taagtttctg ctggcgacga ccgatccaca gaaattgccg gtgacgattt
    16621 tgtcacgctg tctgcaattt catctcaagg cgctggatgt cgagcaaatt cgccatcagc
    16681 ttgagcacat cctcaatgaa gaacatatcg ctcacgagcc gcgggcgctg caattgttgg
    16741 cgcgcgccgc tgaaggcagc ctgcgagatg ccttaagtct gaccgaccag gcgattgcca
    16801 gcggtgacgg ccaggtttca acccaggcgg tcagtgcgat gctgggtacg cttgacgacg
    16861 atcaggcgct gtcgctggtt gaagcgatgg tcgagggcaa cggcgagcgc gtaatggcac
    16921 tgattaatga agccgctgcc cgtggtatcg agtgggaagc gttgctggtg gaaatgctcg
    16981 gcttgttgca tcgtattgcg atggtacaac tttcgcctgc tgcacttggc aacgacatgg
    17041 ccgccatcga gctgcggatg cgtgaactgg cgcgcaccat accgccgacg gatattcagc
    17101 tttactatca gacgctgttg attggtcgca aagaattacc gtatgcgccg gaccgccgca
    17161 tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca tccgcgtatg ccgctgcctg
    17221 agccagaagt gccacgccag tcctttgcac ccgtcgcgcc aacggcagta atgacgccaa
    17281 ccctggtgcc gccgcaatca gcgccgcagc aggcaccgac tgtaccgctc ccggaaacca
    17341 ccagccaggt gctggcggcg cgccagcagt tgcagcgcgt gcagggagca accaaagcaa
    17401 aaaagagtga accggcagcc gctacccgcg cgcggccggt gaataacgct gcgctggaaa
    17461 gactggcttc ggtcaccgat cgcgttcagg cgcgtccggt gccatcggcg ctggaaaaag
    17521 cgccagccaa aaaagaagcg tatcgctgga aggcgaccac tccggtgatg cagcaaaaag
    17581 aagtggtcgc cacgccgaag gcgctgaaaa aagcgctgga acatgaaaaa acgccggaac
    17641 tggcggtgaa gctagcggca gaagccattg agcgcgaccc gtgggcggca caggtgagcc
    17701 aactttcgct accaaaactg gtcgaacagg tggcgttaaa tgcctggaaa gaggagagcg
    17761 acaacgcagt atgtctgcat ttgcgctcct ctcagcggca tttgaacaac cgcggtgcac
    17821 agcaaaaact ggctgaagcg ttgagcatgt taaaaggttc aacggttgaa ctgactatcg
    17881 ttgaagatga taatcccgcg gtgcgtacgc cgctggagtg gcgtcaggcg atatacgaag
    17941 aaaaacttgc gcaggcgcgc gagtccatta ttgcggataa taatattcag accctgcgtc
    18001 ggtttttcga tgcggagctg gatgaagaaa gtatccgccc catttgatcg taagcacagc
    18061 ttacgttcgt catccttaac gtgattgaga gagaaaccta tgtttggtaa aggcggtctg
    18121 ggtaacctga tgaagcaagc ccagcagatg caagaaaaaa tgcagaaaat gcaggaagag
    18181 atcgcgcagc tggaagttac cggcgaatct ggcgcaggtc tggtaaaagt gaccatcaac
    18241 ggtgcacaca actgccgtcg cgtagagatc gacccgagcc tgctggaaga cgacaaagag
    18301 atgctggaag acctggtggc tgcagcattc aacgacgcag cacgtcgtat tgaagaaacg
    18361 cagaaagaaa aaatggcctc tgtatcctcc ggaatgcagc tgccgcctgg ctttaagatg
    18421 ccgttctgat gcaaaccagc ccgctgttaa cacagcttat ggaagcactg cgctgtctgc
    18481 cgggcgttgg cccgaagtcg gcgcagcgta tggcgttcac gctgcttcag cgcgatcgta
    18541 gcggcgggat gcgtctggcg caggcgctca cccgggcgat gtcggaaatc ggccactgcg
    18601 ccgattgccg cactttcacc gaacaggaag tctgtaacat ctgttcgaat ccgcgtcgtc
    18661 aggaaaacgg tcaaatctgt gtggtggaga gtccggcgga catctacgcc attgagcaga
    18721 cggggcagtt ttctggtcgt tattttgtgt tgatggggca tctgtcaccg ctggacggca
    18781 tcggtccgga tgatatcggg cttgatcgtc tggaacagcg tctggcagag gaaaaaatca
    18841 ctgaagtgat cctcgccacc aatcccacgg ttgaaggtga agctaccgct aactacattg
    18901 ccgagctttg cgcgcaatat gacgtggaag ccagccgaat cgctcatggc gtaccggttg
    18961 gcggcgaact ggaaatggtc gatggcacca cgctgtcaca ctccctcgcc gggcgtcata
    19021 agattcgttt ttaagcaaac gagagcagga gcacctgctc tcgcttgaaa ttattctccc
    19081 ttgtccccat ctctccctca tcctgttttt aaccttaaaa tggcattatt gaggtagacc
    19141 tacatgaaag gacaagaaac tcgtggtttt cagtcagaag tgaaacagct tctgcacctg
    19201 atgatccatt ctctctattc caataaagaa atcttcctgc gtgagcttat ctctaacgcc
    19261 tccgatgcgg cggacaagct gcgtttccgt gcgctatcta acccggacct gtacgaaggt
    19321 gatggcgaac tgcgcgttcg tgtctctttc gataaagaca agcgcacgct gaccatctcc
    19381 gataacggcg tggggatgac ccgcgacgaa gtgattgacc atctggggac tatcgctaaa
    19441 tccggtacca aatcattcct cgaatccctg ggttctgacc aagcgaaaga cagccagctg
    19501 atcggtcagt ttggtgttgg tttctactct gcgtttatcg tggccgacaa agtgaccgtg
    19561 cgtactcgcg cggcaggcga aaaaccagaa aacggcgtct tctgggaatc ggctggcgaa
    19621 ggtgaatata ccgttgccga tatcaccaaa gaagatcgtg gtactgaaat caccctgcat
    19681 ctgcgtgaag gcgaagacga gttcctcgat gactggcgcg tgcgttccat catcagcaaa
    19741 tactccgacc atatcgcgct gccggtagag atcgaaaaac gcgaagagaa agatggcgaa
    19801 accgttatct cctgggcaaa aatcaacaaa gcgcaggcgc tgtggactcg taacaagtcg
    19861 gaaatcaccg atgaagagta taaagagttc tacaaacaca tcgcccacga ctttaacgat
    19921 ccgctgacct ggagccacaa ccgcgttgaa ggtaagcagg agtacaccag cctgctgtac
    19981 atcccgtccc aggctccgtg ggatatgtgg aaccgtgatc ataaacacgg cctgaaactg
    20041 tacgttcaac gcgtgtttat catggacgac gctgaacagt tcatgccgaa ctatctgcgc
    20101 ttcgtgcgtg gtctgattga ctccagcgat ctgccgctga acgtttcccg tgaaatcctc
    20161 caggacagca cggtaacgcg taacctgcgc aatgcgctga ccaagcgtgt gctgcaaatg
    20221 ctggaaaaac tggcgaaaga cgacgcggaa aaataccaga ccttctggca acagtttggc
    20281 ctggtactga aagaaggtcc ggcggaagat ttcgctaacc aggaagcgat cgccaaactg
    20341 ctgcgttttg cttctactta taccgattct tctgcgcaga ccgtgtctct ggaagactac
    20401 gtttcccgca tgaaagaagg gcaggagaaa atctactaca tcactgcaga cagctatgcg
    20461 gcagcgaaga gcagcccgca cctggaactg ctgcgtaaga aaggcatcga agttctgctg
    20521 ctttccgacc gcatcgatga gtggatgatg aactatctga ctgagttcga cggtaaaccg
    20581 ttccagtcgg tgtctaaagt tgacgagtcg ctggaaaaac tggctgacga agttgatgag
    20641 agcgcgaaag aagcggagaa agcactgact ccgttcatcg accgcgtgaa agctctgctc
    20701 ggcgagcgcg tgaaagatgt ccgtctgact caccgtctga ccgatacgcc agcgatcgtt
    20761 tcgaccgacg cggacgaaat gagcactcag atggcgaaac tgttcgctgc ggcgggccag
    20821 aaagtgccag aagtgaaata catcttcgaa ctgaacccgg atcacgtact ggtgaaacgt
    20881 gcggcagata ctgaagatga agccaagttc agcgagtggg tagaactgct gctggatcag
    20941 gcgctgctgg cagaacgcgg cacgctggaa gatccgaacc tgtttattcg tcgtatgaat
    21001 cagctgctgg tttcctgatg tcatgccgga tgacctttgt gtcatccggc atttttcttt
    21061 tcatcatctg tactttccgc aaattatctc gccattaacc gtttcagcct caggtgcctt
    21121 tcttgaggca atcgcctgtt ggtggtatcg tttatcgctt tttcaaaaaa atcgacacat
    21181 tttaagggga ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc ggggaaaggg
    21241 actcaggctc agttcatcat ggagaaatat ggtattccgc aaatctccac tggcgatatg
    21301 ctgcgtgctg cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga cattatggat
    21361 gctggcaaac tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg cattgctcag
    21421 gaagactgcc gtaatggttt cctgttggac ggcttcccgc gtaccattcc gcaggcagac
    21481 gcgatgaaag aagcgggcat caatgttgat tacgttctgg aattcgacgt accggacgaa
    21541 ctgattgttg atcgtatcgt aggccgccgc gttcatgcgc cgtctggtcg tgtttatcac
    21601 gttaaattca atccgccgaa agtagaaggc aaagacgacg ttaccggtga agaactgact
    21661 acccgtaaag acgatcagga agaaaccgtg cgtaaacgtc tggttgaata ccatcagatg
    21721 actgcaccgc tgatcggcta ctactccaaa gaagcggaag cgggtaacac caaatacgcg
    21781 aaagttgacg gtaccaagca ggttgctgaa gttcgcgctg atctggaaaa aatcctcggc
    21841 taatcaagcc gcagcagatc tgctttcggg caggtctgct tatccacctc aggcaatacc
    21901 tctcacagca attagttctt cttcctcact tttccgctac aattatcaac aagttgaatc
    21961 gataagaggc ggtaatgcgt cagactaaaa ccggtatcct gctggcaaac ctgggtacgc
    22021 ccgatgcccc cacacctgaa gcggtaaaac gctatctgaa acaattttta agcgacagac
    22081 gcgtggttga tacctcacgg ttgttatggt ggccgttgct gcgcggcgtg attttgccgc
    22141 tgcgctcgcc gcgtgtggcg aagctgtacg cctctgtctg gatggaagac ggctcgccgc
    22201 tgatggttta cagccgccag caacagcagg cgttggcaca acgtttaccg gatacgccag
    22261 tggcgctggg catgagctac ggctcgccat cactggaaag ctccgtcgat gaactcctgg
    22321 cagagcatgt agatcatatt gtggtgctgc cgctttatcc gcaattctcc tgttctacgg
    22381 tcggtgcggt atgggatgaa ctggcacgca ttctggcgcg caaacgtagc attccgggga
    22441 tatcgtttat tcgtgattac gccgataacc acgattacat taatgcactg gcgaacagcg
    22501 tacgcgcttc ttttgccaaa catggcgaac cggatctgct gctgctctct tatcatggca
    22561 ttccccagcg ttatgcagat gaaggcgatg attacccgca acgttgccgc acaacgactc
    22621 gcgaactggc ttccgcactg gggatggtac cggaaaaagt gatgatgacc tttcagtcgc
    22681 gctttggccg ggaaccctgg ctgatgcctt ataccgacga aacgctgaaa atgctcggag
    22741 aaaaaggcgt aggtcatata caggtgatgt gcccgggctt tgctgcggat tgtctggaga
    22801 cgctggaaga gattgccgag caaaaccgtg aggtcttcct cggtgcgggc gggaaaaaat
    22861 atgaatatat tccagcgctt aatgccacgc cggaacatat cgaaatgatg gctaatcttg
    22921 ttgccgcgta tcgctaaagc tgagcggtaa agaactgagc gccgtcgcga agagcctcgt
    22981 cggcggtttt catcatccgt gaataatgca aaaaggcatg cagcgtacct gggtagagtt
    23041 tgaactcaca gggctgctga tgcgccgcta acgtctagta aagcagacgg ctgtcatcca
    23101 gcagcggatc gaactccgcc ccggcaataa aacagggcgg aacttcgcga gtgagatcat
    23161 tattaaacag acagtaatac ggtgactcgc ggtccgcgtc gttgcttaaa tacgcctctt
    23221 catacatctg caaatcctgt tgcgttaagc catcccagac accgcccaac agacgacgag
    23281 tcacggaatc ccgtaatccg taaagcccat accacagcaa aacacccgca attttaccgc
    23341 aatcgatctg tttatcacgc aaccacaacg cactggcgag cgccagcatg gcacctgcgg
    23401 aatcaccggc aaagccaatg cgggacatat tgatttgata atcctccgcc tgctggtgga
    23461 aataacaaca agcagccaca atttcctcta tcgcttgtgg aaaacgcgct tcaggtgaaa
    23521 gggtgtaatt aataccaatc accgtacatt ggctgtagct tgccagcagg cgcatgatgc
    23581 gatcgtgggt atcgagattg ccgagaataa aaccgcctcc atgcaaataa aatagcgtcg
    23641 ccgggctatc tggctgcgga caaaagagac gtgtttccac ctgcccatat tttgttggaa
    23701 ccatgtaagc tctggttgcc atttctggag cgcccgcatt ccagaatcgg cgctcaagcg
    23761 tgtaatactg tcgttgctca gcaatcgttc ccgttgcggg ccaggacggt aaatccggct
    23821 gaagagtatt cacaacggtc ttcatttcag cagaaataag gtccagaaca ggtagtttgt
    23881 tttccggctt catacaaaaa ctcctttcaa attacgtcat tgtaaggaac cactgccatg
    23941 aaaatgcgat cccgcctgct gatattgaaa ctggctgcgt ctcgcgcgct cccgtcagat
    24001 tgtgttaaca ttcgccgctc agttaaccac ccgtaaaaac aaccatgaaa tttcccggta
    24061 aacgtaaatc caaacattac ttccctgtaa atgcacgcga tccgctgctt cagcaattcc
    24121 agccagaaaa cgaaaccagc gccgcctggg tcgtaggtat tgaccagacg ctggtcgata
    24181 ttgaagcgaa agtggatgat gaattcattg agcgttatgg attaagcgcc gggcattcac
    24241 tggtgattga ggatgacgta gccgaagtgc tttatcagga gctaaaacag aaaaacctga
    24301 ttacccatca gtttgcgggt ggcaccattg gtaacaccat gcacaactac tcggtgctcg
    24361 cggacgaccg ttcggtgctg ctgggcgtca tgtgcagcaa tattgaaatt ggcagttatg
    24421 cctatcgtta cctgtgtaac acttccagcc gtaccgatct taactatcta caaggcgtgg
    24481 atggtccgat tggtcgttgc tttacgctga ttggcgagtc cggggaacgt acctttgcta
    24541 tcagtccagg ccacatgaac cagctgcggg ctgaaagcat tccggaagat gtgattgccg
    24601 gagcctcggc actggttctc acctcatatc tggtgcgttg caagccgggt gaacccatgc
    24661 cggaagcaac catgaaagcc attgagtacg cgaagaaata taacgtaccg gtggtgctga
    24721 cgctgggcac caagtttgtc attgccgaga atccgcagtg gtggcagcaa ttcctcaaag
    24781 accacgtttc tatccttgcg atgaacgaag atgaagccga agcgttgacc ggagaaagcg
    24841 atccgttgtt ggcatctgac aaggcgctgg actgggtaga gctggtgctg tgcaccgccg
    24901 ggccaatcgg cttgtatatg gcgggcttta ccgaagacga agcgaaacgt aaaacccagc
    24961 atccgctgct gccgggcgct atagcggaat tcaaccagta tgagtttagc cgcgccatgc
    25021 gccacaagga ttgccagaat ccgctgcgtg tctattcgca cattgcgccg tacatgggcg
    25081 ggccggaaaa aatcatgaac actaatggag cgggggatgg cgcattggca gcgttgctgc
    25141 atgacattac cgccaacagc taccatcgta gcaacgtacc aaactccagc aaacataaat
    25201 tcacctggtt aacttattca tcgttagcgc aggtgtgtaa atatgctaac cgtgtgagct
    25261 atcaggtact gaaccagcat tcacctcgtt taacgcgcgg cttgccggag cgtgaagaca
    25321 gccaggaaga gtcttactgg gatcgttaag ttatcgtcgg ttcgtaggcc agataaggcg
    25381 ttcacgccgc atctggcatt tggctctcga tgcctgatgc gacgctggcg cgtcttatca
    25441 tgcctacata tttttcatat tttacatccg gcaaccaccg tttaccccgt caccacttca
    25501 cccgccggtg gcgtttccag cagttccagc atagtacggg caatttcacg ttcacccatc
    25561 actacctgat tcgcgccacg ttcggtgata tacgtcactt catcgtcata atgggcgcgg
    25621 gcgataatct caatatccgg gtttttcgcg cgggcagacg ccacaatctc acccgcttca
    25681 taaccgttgg ggatcgtcag gatcagccat tttgcacatt ccagatgtgc cagttgcata
    25741 atttcttcgt tcgctgcatt gcccaataca gcgcggaccc cacgctctcg cagctcatca
    25801 acacgggttc gtgacgtctc aatcaccacc agcggaatat cagaggcgag caatttctcc
    25861 cccagcaggc tgcctacacg accgtaaccc accaatagcg catggttgca aatatccact
    25921 gggatctgtt tctcttcttc gattgcctct tccagcgtct gctcttccag cgtttcggtc
    25981 ttcgccagat atttctccag tagtgcgaac agtaccgggt tgagcataat cgacaggatc
    26041 gcccctgcca gtaccaggtt ttgtccggcc tgcggcagta aattcaatgc cattcccagt
    26101 cccgccagga taaacgcgaa ctcaccaatc tgcgccaggc tggcggcgat ggttaatgcc
    26161 gtacgttggg agtgaccaaa cagacggaca aggaacaggg ccgcgaccga tttaccaaac
    26221 agaataattg ccagcgtcgc cagcactgcc agcggttgct gaatcagaat taacggatca
    26281 aacaacatcc cgactgagac aaaaaacagc accgcaaacg cgtcgcgcaa tggcaacgta
    26341 tcgtgggcgg cacggtgact caattcagac tcgttcagta ccatcccggc aaagaacgca
    26401 ccgagtgcaa aggagacatc aaacagctct accgcaccaa aggcaatccc taacgccagc
    26461 gccagcactg acagggtaaa cagctcgcga gaaccggttg ccgcgctgcg tgccataatc
    26521 cacggcacca gacggcgacc taccagcatc ataatggcga taaatgcgat caccttgccg
    26581 atggtgatcc ccatatcgac tgcaagagtg gcaaagccca catcgccctg ttccatcatt
    26641 cctgccactg cgggcagcaa caccagcgtc agaaccatca ccaggtcttc cacaatcaac
    26701 caaccgatgg cgatttgccc acgctgactg tctattaatt gccgttcttc aagtgcgcgc
    26761 agtaacacca cggtactggc ggtggaaaga cataagccga acacgatacc ggtcattaac
    26821 gaccagccca gcacggcgga gagcgccata cccagcagcg tcgccacggc tatctgggcg
    26881 atcgcgccgg gaatggcgat ggactttacc gccatcaaat ccttgagcga aaaatgcaaa
    26941 ccgacgccga acatcagcaa aataacgccc agttcagcca gttccggcgc aagcttggta
    27001 tcggcaacaa agcccggagt gaatggtcct gccagcacac ccgctaacag atatcccacc
    27061 agaggagaaa tacgtagttt attggccagc atgccgagga taaaggcgag cacaaggccg
    27121 ccaacaatgg tggtgataag cggggtggcg tgatgcattc cgtctccttt tcctggtggt
    27181 tattgtccat ttttggctgg gaaaaccaaa attacaggta atagtttatg acaatttcat
    27241 tgatgatgtt tatgaataat tgttgaattt tgcagaaaaa tggaattagc tgcaaaaaaa
    27301 gcacggttcg gaaaacagaa ggcggtttaa cagaggaaag gcttatggcg actgtaaaac
    27361 gatgcagcca aagtttgcct ttggctgcaa tgaaatcagt ctttatgccg gttatcaggc
    27421 aggaatatgg tcaacatccc caatagtggc aggaaagcac agattttata gactaactcg
    27481 atgctggtgt gatcggcgat aagccccaga actgccgctc ccagacctcc catgccgaaa
    27541 gcaaaaccga aaaagagtcc agaaaccata ccgatacgtc ccggaagtag ctcctgagcg
    27601 tagaccagaa tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc
    27661 cccgtccagt gcaggcgggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata
    27721 gagccccaaa tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta
    27781 cctgccgcaa cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat
    27841 ccgaattttt gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat
    27901 ttcgagaaaa tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt
    27961 ggattgatta tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa
    28021 cggctaattt gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg
    28081 ttgcctttgc cataaggcgc gataatcacc gccgccagca agggtcccag ggaactgcca
    28141 aagttgccgc cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata
    28201 cgggctacgc gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgct
    28261 gccagcagaa ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta
    28321 aagcacatgc caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc
    28381 actggttgca gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata
    28441 aatgtcagag aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc
    28501 atgtcgttca gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc
    28561 ttggtcgttg acgcagccgc gcccgccaca ggctgggttt gttcactcat tgccatagga
    28621 aagtcacttt ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact
    28681 aacatacctg tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct
    28741 ggatgcagat ttatcttcac cggacgcaga cttgtctatg atgttgcgtc atactatttt
    28801 tcaacacgtt gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg
    28861 ttagcgctgt taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat
    28921 aaaacctaca aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat
    28981 gaatatggcg aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag
    29041 gttgcggctg aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg
    29101 cccgagtctg acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat
    29161 gacgcgatgg cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag
    29221 gaaaagtggg ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag
    29281 cgcctgttta aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg
    29341 ctgacaaccg atgacacagc aaaaattggt aacctggaat acttcactga tatcgaattt
    29401 cgtaagcccg ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca
    29461 gacattatta tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac
    29521 gcaccgggcg atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc
    29581 ggtggtcact cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac
    29641 gtgccgggta cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat
    29701 gagtggggca aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg
    29761 gttaactacc agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa
    29821 agcgagcgcg tgctttacac tcctgaaatc gctgaaaacc agcaaatgat ctcgctgtta
    29881 tcaccgttcc agaacaaagg caaagcgcag ctggaagtga aaataggcaa aaccaatggt
    29941 cgtctggaag gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt
    30001 ctggcagccc aaatggatcg cactggtgcc gactttgcgg tgatgagcgg aggcggaatt
    30061 cgtgattcta tcgaagcagg cgatatcagc tataaaaacg tgctgaaagt gcagccattc
    30121 ggcaatgtgg tggtgtatgc cgacatgacc ggtaaagagg tgattgatta cctgaccgcc
    30181 gtcgcgcaga tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg
    30241 gcgaaagacg gcaaactgaa cgaccttaaa atcaaaggcg aaccggtcga tccggcgaaa
    30301 acttaccgta tggcgacatt aaacttcaat gccaccggcg gtgatggata tccgcgcctt
    30361 gataacaaac cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat
    30421 atccagaaaa gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg
    30481 cagtaatccg aaagtgccgg atgtttgcat ccggcacaat gcttaatcgc ggcgggcgat
    30541 atcagcaaat ttggcatcga ggatctttgc cagatcgcct gccgccagtt cgatatccag
    30601 tccgcgcttg ccgccggaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat
    30661 cgttggcaga cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga
    30721 acgctgcgcg accatcggat cggccatctc aactttcttg gcacccagcg cttttgctac
    30781 ttttttaaga tccagttgac ctgcgaccgg cgtaacggcc acggcaaggt gtttcatatc
    30841 accgttcact gccaccagca gcgttttgta gacctgatcc ggattcaaac ctaatttttt
    30901 gacgacttca tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa
    30961 cgaaatcttg tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta
    31021 aacaaaaaaa cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc
    31081 taagagtatt ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt
    31141 gacggcataa taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca
    31201 tttttttagc gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc
    31261 gcgaccacat atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc
    31321 tgatgcatca gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta
    31381 ttttgcggcg gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta
    31441 tgccagtggg tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac
    31501 atggcaatct ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc
    31561 gcctgcaata gctggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc
    31621 gtcgcctgaa gaaccatcgc gatttgccac agcggttggg tagaaaagag tgagggagaa
    31681 atgcccatct cctggctaat gtgctgcagg ttttggagtt gctcctcgct aatgcgctct
    31741 tccagcgcct cgcaggcagg caaattagca aaaggcgtat cgctggtgga aacatccgcc
    31801 tcgacgatca gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc
    31861 atatcgtggc tacccatatg aatactgcca atcagatgaa aatggcgatt gccggggagg
    31921 gtgatatcga tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt
    31981 acccggtaca acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga
    32041 gggatggcgc aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct
    32101 tgcatcgcta ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc
    32161 gacgacaccc ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa
    32221 tgagttagca gagttgctgc atgttcatcg taatcgcgtc agtgcactga tcaataacaa
    32281 tcgtaaactc accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga
    32341 ttttaggcta aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac
    32401 ccaggaagaa ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg
    32461 tgcaaaaaag gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt
    32521 cacgccgcat ccggcatccg agcaccattg cctgatgcga cgcttacgcg tcttatcagg
    32581 cctacaaacc tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc
    32641 atgcgtcgat gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc
    32701 ttcggtttaa accgcagcaa ccggttggcg ttacttacta cggtaattga cgagagcgcc
    32761 attgccgctc cggcaactac cgggttaagc agtgttccag taaacggcca caaaataccg
    32821 gcggcgaccg gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg
    32881 ttgcgcagcg ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc
    32941 atcagggtaa tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg
    33001 acatccgcct gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga
    33061 cgtccttcac tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca
    33121 atcacttcat caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg
    33181 gtcaacatca ccagacgata tcccgcttta tgcaggcgtt gcagcgccgc cacgctatca
    33241 ctacgcaacg gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc
    33301 acaggcgttg ctccttgcga tgcctgagct gaaatatcgg cttcaatcgc tttagtatcg
    33361 acttgctgat catttaacaa cgcctgatta cccagcaata acgcatgacc ttctgcttca
    33421 ccgctcacgc ccagcccgcg caatgtgcgg aaaccgttga cctgcggtag ctgcatatcg
    33481 cctgctttat caaggatcgc tcgtgccaat ggatggctgg aaccttgctc cagcgccgcc
    33541 gccagacgca gcgcctgcgc ttcgtcaaca tcagcaaatg ttttcactgc gacaacctgc
    33601 ggcttccctt cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg
    33661 cgttgcagcg cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg
    33721 gaaataatcg acatcggcgt cgccagtccc agcgcacacg ggcaggcgat aatcagcacc
    33781 gtagtggcaa tcaccagggt atagacaatc tgcggtgccg gaccaaagaa ataccagatt
    33841 gccgcactga caagcgcaat aaccactact accggcacaa atatggctga gattttatcc
    33901 gccagctgac cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatcctt
    33961 gatagcgtgg tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc
    34021 actgtcccgg catggacgct atcgccttcg cctttttgct gcgggattgg ttcgcccgtc
    34081 agcatcgctt catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga
    34141 tcgccggtcg tcaggcgcag caacatacct ggctgcactt ctgccagagg cacgcttttt
    34201 tcaccttcgt cagtaaccag gcgcgccgtc ggtggggtta aatcgagtaa tttctccagt
    34261 gccttagaag aacgctgacg tgcgcgtgct tccagcatat ggccgagatt gatcagaccg
    34321 ataatcatcg cactggcttc atagtaaaga tgtcgcgctt ccatcgggaa ccactgcggc
    34381 cacaggttga cgctcatcga atagagccac gccacgcccg ttcccagcgc caccagcgta
    34441 tccatcgtcg ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgctg
    34501 gcgaaaacca tcaccgccag ggttatcagg ccgataacca accacaggct gcggttgtcg
    34561 gcggtgacta tcatgttatc gccgatcatc ccccagacca tcaccgggat acccaccgcc
    34621 agtgcgacaa ttgcctgcca gcggaagcgc ttcatcgtag cgacggcggt ttcttgctgg
    34681 cgttcgcggc gtttcgcgtc atcttcaatc gcttccgcgc cgtagcccgc tttttccacc
    34741 gcctgcacta aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg
    34801 tttacccgtg cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca
    34861 cagctggcgc agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc
    34921 gctgccggaa gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc
    34981 agcggtttag cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg
    35041 atcagctgtt ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgct
    35101 tgttccacat ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag
    35161 gacaggccgt ccaggttcag gtcgatagtt tgtgacataa aacactcctt taagacagtt
    35221 ttgactggct gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga aattaataaa
    35281 ccaggcgggt aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac
    35341 atttatttct ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg
    35401 aaaatcggca ctggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga
    35461 tgttagatgc aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac
    35521 ttaacggcgg acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg
    35581 cggcagtggc tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc
    35641 gctttgcact ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg
    35701 gcccgcaggc ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag
    35761 ttatcgcctt agagttgcat ggcggcaaac cgctttcgcc actggtaaat gctggcgcta
    35821 ttgccaccac cagcctgatt aacgctgaaa acgccgaaca acgctggcag cgaattttac
    35881 atatccaaca gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg
    35941 aacaaacaac caacttccat aatcgggcca ttgcctggct gctgtactcc gccggatatc
    36001 tctattgtga tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca
    36061 atactgttga actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga
    36121 cgcataaacg cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg
    36181 aagggctgta tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg
    36241 gtgtaggtgg cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac
    36301 caccgctgga cgaagaaggc aatagtgttc gcggtcaaaa aatggtggca tcggtcgcta
    36361 agcaactcgg ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg
    36421 taacaaaccc ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc
    36481 ggggatcttc gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctggggcgc
    36541 ctttgctttt ggcggtattg tggctatgtt ttccggttat gcctatgcgc gtctgggggc
    36601 gagctatccc agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt
    36661 cttttcgcta gcgctctcgt tgctatacct gttgacgctg gcggtgagca tcgccatggt
    36721 tgcccgtgct tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca
    36781 ccttattttg ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag
    36841 caaccatgcg gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt
    36901 attgctgatt attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc
    36961 ccccagctcc ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt
    37021 tggcatgatg gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc
    37081 gtttctggtg gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct
    37141 tagcgatgta tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc
    37201 ttctccgctg ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac
    37261 ggcttcaggc attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag
    37321 cgaacgcgaa ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa
    37381 tatcatcgtc gtggtgttga taatgctgat gacggcggcg ctaaatttag actcactcgc
    37441 cagcgttgcc agcgccacct ttttgatctg ctatctggcg gtgtttgtgg tggcgatccg
    37501 cctgcgtcat gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt
    37561 gttggtgatc gttggcttta tctacagcct gtggtcccag ggcagccgtg cgttgatatg
    37621 gattattggt gcaatcttac tcagccttat tgtggcaatg gtcatgaagc gtaataaaac
    37681 cgtataacac ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg
    37741 cactggcaaa aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg
    37801 ttgcgcgaac gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca
    37861 ttcgcttcta tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc
    37921 gcacctacac gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg
    37981 gctttaacct ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca
    38041 gcgccgacgt caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg
    38101 agctgcaatc catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca
    38161 gcgccgactg cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat
    38221 taagacgaga cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatgc
    38281 gtacctgcac cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata
    38341 tgaccgcgcc cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc
    38401 ccgcgctggt ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat
    38461 ttccaccaca gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc
    38521 caacccagcg gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac
    38581 aacaaataac catttccgcc cagcatctcg gctgccagca gcaacccgcc gagactgagc
    38641 cagaaaatat gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc
    38701 tgtctttcac cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg
    38761 gcatcatcac tactttgctg ttactggagg aaccgatctg ctgtaacgct tcggtgtatt
    38821 tttgcgctac gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca
    38881 ttttggtggc gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg
    38941 ccgactgacg ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga
    39001 ggatttccgc ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg
    39061 ctttcatctg cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt
    39121 caatacgggt gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc
    39181 gtgagttgat gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg
    39241 tacggatgtt agtcatggtc aggttgatga tcgccagctc cagattgctg acttcatagg
    39301 cagcgcgtgg cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt
    39361 tatctttcga gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct
    39421 tgcgaccaat gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg
    39481 tatagcgacc aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg
    39541 cgccgacaat gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa
    39601 cctcctgttg taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg
    39661 attactgaaa gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc
    39721 agaaaaaggg aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat
    39781 atctggcggg tgatacgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat
    39841 ttaagttaat taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt
    39901 cattgatcag cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa
    39961 aaccagaaat ctaccgccag caagtctctt actgcgccca gacgccgacg ctgtttggcg
    40021 atacggtata cgataatctg atctttccct ggcagatccg tgaccagcag cctgacccgg
    40081 ccatttttct cgattttctc gaacgcttcg ctttgccgga cagcattttg acgaagaata
    40141 tcgccgagct atctggtggt gaaaaacaac gcatctcatt gattcgtaac ctgcaattta
    40201 tgccgaaggt tttattgctg gatgaaataa ccagtgcgct ggatgaaagt aataaacata
    40261 acgtcaatga gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga
    40321 cacacgataa agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg
    40381 gagaaatgca ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg
    40441 gcattaatgc tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag
    40501 aaagatattc tatggagcgt cgggcgagcg ataattcagc tgattattgt cggctatgtg
    40561 ctgaagtata ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc
    40621 tgctttaatg cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc
    40681 tcatcgttta ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca
    40741 ggggcgattg aatttatccc gatgcaggtg atccctgtcg ccgggatgat tgccggtaac
    40801 gccatggtag cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag
    40861 caacagattc aggagaaact gagtcttggc gcgacgccga agcaggcttc agcgatattg
    40921 attcgcgaca gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc
    40981 ttagtgagtt taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag
    41041 gcgattaaat atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc
    41101 attattgcct gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg
    41161 actcaattga agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg
    41221 ttcatgtgcc gaacaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc
    41281 gacgccagtg catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc
    41341 tgaccgtctg cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct
    41401 tcattgcgcc caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct
    41461 ggattctccg ccacctgctg ttgcaactgt tgaatttccg gcttatcagc cgcctgcttc
    41521 agcagttcga tttgcgctac cagcccctgg tagcgggtgt cctgatcctg caacggaatg
    41581 gttttcagca ccgcttccgc atcttcagaa cggttcagcg caatcagcgt ttctgccagc
    41641 agcaggccga tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg
    41701 gcatcggtgt aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct
    41761 tcgcgcggca gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg
    41821 aagccatcta ccggttgccc gttctggaac agatacacgg tcggaatcgc gcgcagacca
    41881 aactgggcgg caatcatctg ctccgcgtcg cagtccagct tcgccagaat aaattgcccg
    41941 ttgtactgcg ccgcgaggct ttccaggatc ggggttaact gcaaacagtg ctggctacgt
    42001 tcagaccaaa aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg
    42061 ttagattcgt taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat
    42121 attttctttg acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc
    42181 atcacgcgcc caggcagcag gcgctcgagc accattactg cccaggtcac cagcgttacc
    42241 ggatagcgca tcttcggctt ctcgctaata aaagcatggc gcactttgtc gaccaccgct
    42301 tccggtccca acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc
    42361 gtctggttga cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact
    42421 ttaattccgc tgtggcgtag ctccatgcgc agcgcgtctg accacgcctc cagcgcatat
    42481 ttactggccg cgtaagcgcc acgacccggc gtggagatta atcccatcac cgatgatgtc
    42541 atcacaatac gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg
    42601 tgtgcgccga aaaagttggc ggaaaactgc tgttccatct gcgcacggct gatggtggaa
    42661 agggggccat acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc
    42721 agggcgatca cctcgtctgc tgcgcgatta acactttctg gtgaatccag atcgatcaac
    42781 acgccggtaa atcccatgtt gttcatacgc tcaacatcat tcggttttcg gcaacctgcc
    42841 agcacatgaa aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa
    42901 catccggtaa ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta
    42961 tgagtcatga tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg
    43021 ggcgtcgcgg ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc
    43081 ttccataaaa aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc
    43141 gctaaaggct tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa
    43201 tggttcagcg ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg
    43261 ctgtggctga aaaccacgca aaccatcatt gcgcccagtt caaccagcac ccaacgcggc
    43321 tgatgctgtt tgagcagagc cggaaggcgc gccagccctt gttgcgaggt gtcgccgctg
    43381 atgctggcat taactaccga cgttttactc tgccacttat cattcaacaa ggcaggccag
    43441 gccgcgctgg cagacattcg atacccggcg ctcaggctat cacccagaat caataacgtg
    43501 tccgctgcgg cggcacggaa ggttaacagg accaggaaca ggaagggcaa atgccagcgg
    43561 aaaacattgt tgaagttcat catcttaaga agtccgtcgg tcagggggag catgaactct
    43621 ccatcctcac cggagttgag ctggttgtca aacgtggcga gaccatcgca ctggtgggcg
    43681 agtcgggatc gggtaagtca accttgctgg cgatcctcgc cgggcttgat gacggcagca
    43741 gtggcgaagt gagtctggtg ggacaaccgc tacataatat ggacgaagaa gcgcgggcaa
    43801 agttgcgcgc gaagcacgtc ggctttgttt ttcagtcatt tatgttaatt cctaccctta
    43861 acgcgctgga aaacgtcgag cttccggctc tgctgcgcgg tgagagtagc gcggaaagtc
    43921 gtaacggggc gaaagcgttg ctcgaacagt tagggctggg taaacgtctg gatcatcttc
    43981 cggcacagct ttccggcggt gaacagcaac gagtggcgct ggcacgagcc tttaatggtc
    44041 gacctgatgt gctgtttgcc gacgaaccca ccggcaacct tgaccgccag acgggcgata
    44101 aaattgccga cctgctgttt tccctcaacc gtgaacatgg caccacgttg attatggtga
    44161 cccacgacct gcaactggcg gcacgctgcg accgctgctt acggctggtg aacgggcagt
    44221 tgcaggagga agcatgattg cacgttggtt ctggcgcgaa tggcgttcgc cgtcgctatt
    44281 aattgtctgg ctggcgctaa gcctggcggt ggcctgcgtg ctggcgctgg gcaatatcag
    44341 cgatcgcatg gagaagggct taagccagca aagccgtgag tttatggcgg gcgatcgggc
    44401 gttgcgcagt tcacgcgaag tgccgcaagc gtggctggag gaagcgcaaa agcgcggcct
    44461 gaaagtcggc aagcagctga ctttcgccac aatgaccttt gcaggcgaca caccgcagct
    44521 ggcgaacgtc aaagcggtgg atgatatcta cccgatgtat ggcggtctgc aaactaatcc
    44581 ccctggcctg aaaccgcagg cgggcagcgt attgctggcc ccacgcctga tggcactgct
    44641 taacctgaaa acgggcgaca ccattgacgt gggcgatgcc accttgcgga ttgccggaga
    44701 agtgattcag gaaccggatt ccggttttaa ccccttccag atggctccgc gtctgatgat
    44761 gaatctggcg gatgtcgata aaaccggagc cgtgcagccg gggagtcggg tcacctggcg
    44821 ttataaattc ggcggcaacg agaaccagct cgacggctat gagaaatggt tgttacctca
    44881 gcttaaaccc gaacaacgct ggtacggtct ggaacaggac gaaggcgcgc tggggcgatc
    44941 gatggaacgc tcgcaacagt tcctgctgct ttcggcgctt ctgaccttgc tgctggcagt
    45001 ggcagcggtg gcggtagcga tgaatcatta ctgtcgcagt cgctacgatc tggtggcgat
    45061 cctcaaaacg ctgggggcag ggcgagcgca actgcgtaag ctaatcgtcg gtcagtggtt
    45121 gatggtgctg acgctttcag ccgttaccgg tggggccata ggcctgttgt tcgaaaacgt
    45181 gttgatggtg ctgctcaagc ccgttctgcc tgctgcacta ccgccagcca gcctctggcc
    45241 gtggctgtgg gcgcttggca ccatgacggt catctcgctg ctggtggggc tacgaccata
    45301 ccgcttgttg ctggcaacgc agcctttacg cgtattacgt aatgatgtgg tcgcgaacgt
    45361 ctggccgctg aagttttatc tgccgattgt cagtgtggtg gttgtgctgc tgctcgccgg
    45421 attaatgggt ggcagcatgc tgctttgggc ggtgctggcg ggcgcggtag tactggcttt
    45481 gctgtgcggt gtgctgggct ggatgctgct gaatgtactt cgccgcatga cgctgaaatc
    45541 gctgcctctg cgcctggcgg ttagccgcct gttacgtcag ccgtggtcaa cgttaagcca
    45601 gctttcggca ttttcgctct cctttatgct gctggcactg ctgctggtgc tgcgtggcga
    45661 tctgctcgac cgctggcagc agcagctacc gccagaaagc ccgaactact ttttaattaa
    45721 catcgccaca gaacaggtag caccgctaaa agcgttcctc gcggaacatc atatagtccc
    45781 ggaatcattt tatccggtag tgcgggcgcg gctgacggcg attaatgata agccgacaga
    45841 aggtaatgaa gatgaggcgc ttaatcgcga actcaatctt acctggcaaa atacgcggcc
    45901 cgatcataat ccgattgtcg ccggtaactg gccgccaaaa gccgatgaag tgtcgatgga
    45961 agaggggctg gcgaaacgct taaacgttgc cctcggtgat accgtgactt ttatgggcga
    46021 tacccaggag ttccgcgcta aagtgaccag cctgcgcaaa gtggactggg aaagtctgcg
    46081 gcctaatttc tattttattt tccctgaagg ggcattagac gggcaaccgc agagctggct
    46141 taccagtttc cgctgggaga atggcaacgg catgttgacg caactcaacc gccagttccc
    46201 gaccattagc ctgttagaca ttggcgcgat tttaaaacag gtcggtcagg tgctggagca
    46261 ggtaagtcgg gcgctggagg tgatggtggt gctggtcacc gcctgcggta tgttgctgtt
    46321 gctggcacag gtgcaggtgg gaatgcgcca gcgtcatcag gagctggtgg tatggcgcac
    46381 actcggtgcg gggaaaaaac tgctgcgcac gacattgtgg tgtgatgttc gccatgcttg
    46441 ggtttgtttc cggcctggtg gccgcaattg gtgcggaaac ggcactggca gtgttgcagg
    46501 cgaaagtgtt tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca
    46561 gcggtgcgct gctgctgtcg cttttcggcg gctggctggg tgcgcgactg gttaagggta
    46621 aggcgctgtt caggcagttt gtggggtgat gacaggtgtt agattgcctg atgcgctacg
    46681 cttatcaggc ctacgaagag cgtgcaatat attgattttg caagattgtg taggccggat
    46741 aaggcgttta cgccgcatct ggcatgaaca gtgtgcattt tggcaataaa tatgaaacca
    46801 actgtcatgt cggttttttt aaaggccgaa ggaggtaaac cttcggcctt agctacgttt
    46861 cctcagttat tcaataaact gacggatcag ttccagcagc gcgattagcg ccttaagaac
    46921 gatgagaact ttatcgagaa aagttttcat ccgttgctcc cttaagggag aaagacaatc
    46981 acagcttacc cttacgttgc ctataactat gattttcttc atgtgctaga tgaggactgt
    47041 ctctctccaa attagagcac cagaatcagc accacctgta tcctgaccag gacttaaaag
    47101 tacttttccg cactgcggta atcccttgag gcagtatcac tactagcgtc gggattgtat
    47161 cgccaacata gctgtttgaa tatgtaggca tcctgactta taattcactt gctagatgag
    47221 gactgtttaa tttctgcccg gaaccaccaa gtcttgggcg gaaaaaataa aaaaaccgac
    47281 tgtcatgtcg gtttttttat atctgtattc tggtgcaacc attttgttct cacgacaatc
    47341 gccgtttcag aataccgccc atcactgcgc cttaacccat accgccactt ccgcccactc
    47401 accgcgaaag acaacttttt ccgctttttt ctcaagctga tagcgataca ttgggtcgta
    47461 atattcttta agtaacggca ccagccaggc cagatgaccg tcggtgctgc cggtggtgag
    47521 ttgcgttgtc agcgccgtat ccagctgggc agcgagttcg ttatagcgct gtaaccccag
    47581 ccgacgctta atcgccgaaa gtccgtgatg caggtattcg caatattcct gccagccctg
    47641 ttcgtcgcca taagcgtggg taaaatcatg atgcatacgc aagaaatact cttcattcag
    47701 gcgttcaaga cggatctcaa acggatcttc taccaccgca atcgccgcct gagtcattcg
    47761 ctcgcgcaga cattccggca ggtgattcga accgatcatc cggctttcat cttccagcac
    47821 ccacaggcgc aaatcctgac gggcgtcggt ttttagcatt tcggcagcca gcaggttttc
    47881 aaaactcgcc tggctaagtt gtggttgtaa cgtgcgacca aacgccgaac cgcgatgacg
    47941 cgccagccct tccagatcaa caccgtttgg ctgttgttgc actaatagcg ttttaccgct
    48001 gccggtacaa ccgccaatca gcactatcgg tttttgtgcc agttcaatgg tcgcctgaat
    48061 cgcggtctgg cgcagtgcct tataaccgcc ttccaccagc ggataatcaa tccccgcttc
    48121 atgcagccag cgttgcacaa tatgtgaacg ttgaccgcca cgggcgcagc agagaatacc
    48181 ttgcggattt tgcaggcacg ctgcccgcca ggcgtacata cgctgctgac gaatttcacc
    48241 cgccaccagt ttatgcccca gcgccagcgc tgcgtctgag ccttgctgtt tatagcaggt
    48301 gccaacggag gcgcgttcat cgttatccat taacggcaga ttgatagcgg cgggcattgc
    48361 gccttgctca aactcgatag gggcgcgaac atcaataatg ggcgtatcag caatcagcag
    48421 ggcacgatag tcctgttccg tgtgtctctc ttgcatagtt aaaagtgaac ctcaaatcag
    48481 cttgcgcgct attttacgcg ccaacgcgca aggaaacttg atttttaact gcgtggggtg
    48541 ccgaaaattt ctagaaatcc gctgatttcc ggcctgtgtt gggtaaacaa ggtcacaata
    48601 tcttctaccg ctttgcagct gccaaatttg cgccatgcca gactcaatgg cgaaggaggg
    48661 cgcatcgttg ggattacccg gctgaccagt tgttgattat cgatcattga ctggcaaagt
    48721 gattttggca aaaaaccaat gccaacaccc gccagatggg cggcgatttt cgtttccata
    48781 tcgggaacaa taatctcttt ttgccccggc aatcgccagg cgacgcgttt ggttaaggtg
    48841 ccggcgctgt cttcaatatt gaccgccgga aagcgacgca actgcgcttc tgttagcggc
    48901 tcttcaacgt tcgccagcgg atgatccgcc gccatgacaa atcgccattg caccgatcct
    48961 aagggatcaa gactaaaggt atttgccagc gcctcagttc ccgtgacccc gatagccagc
    49021 gaaaaacctt cgtacaatag cgagtcccag acgcccatat agatttgtcg ggagatgtga
    49081 aactgggtaa aggggtaacg ctcattcagc cacgccagca actgggcgac ggcctggggg
    49141 ttatagagca ggttgttgat gacaatattc acctggcgtt ccacgccatc attcacctgt
    49201 tgcagctcgc tgggcatact ttccagccag ctcagccagt ctctggcctg ggaaagtaga
    49261 tgctcgccag ccgctgtcaa cgttacgcta cgagtcgtac ggaaaaacag cgctactccg
    49321 gtattctctt ccagaagttt aatgcgataa ctgatcgtcg ccgtggtttt acataatcgt
    49381 tctgccgctt ttgaaaaact tcctgtttca gcaaccgcaa tgaaagtccg caaggtttct
    49441 ggatcgaaca tcttcaggta tcccctttta aatccgcaag ttgcgtgatt ttcttatcct
    49501 ctgatttatc agtattttta catgataatc ctgttcaatt tgtggactaa atctagtttt
    49561 agaaaaacat tccaactttt gtattaatgt tgttctctta aggttttaga ttgcctgtta
    49621 ttgaaaccaa gctgaccggt cggcggtggt tgaacggaat tatgttacaa ggacaaaaag
    49681 atgaaacttc aggtattacc gttaagtcag gaagccttta gtgcttatgg cgacgtaatc
    49741 gaaacgcagc aacgggattt tttccatatt aacaatggcc tggtggagcg ttaccacgat
    49801 ttggcgctgg ttgagattct tgagcaagac cgtacgctta tcagcattaa ccgcgcgcaa
    49861 ccggcgaatc tgccgctgac cattcacgaa ctcgaacgtc atccgctggg cactcaggcc
    49921 tttatcccaa tgaaaggtga agtgtttgtg gtggtcgtgg cgttaggcga cgacaaacca
    49981 gacctgtcaa cgctgcgggc gtttatcacc aacggtgaac agggggtgaa ttaccatcgt
    50041 aacgtctggc atcacccact tttcgcctgg cagcgcgtca ccgattttct gaccatcgat
    50101 cgcggcggca gtgacaactg tgatgttgaa agtattcctg aacaggaact ctgttttgcg
    50161 tgacgcctgc aaccgacttg cataagataa actaattgtt cattgtttat gctcacttgt
    50221 aggtcggagt taacgtaggt atgacggaag ttagacggcg cggcaggcca ggacaggcgg
    50281 agcctgtggc acagaagggc gcacaggcgt tagagcgggg aattgcgatt ctgcaatatc
    50341 tggaaaaaag tgggggaagt tcgtcggtta gcgatatttc tctcaatctg gatttgccgc
    50401 tctccacgac ctttcgcttg ctgaaggttt tacaagcagc ggattttgtc tatcaggaca
    50461 gtcaattagg ctggtggcat ataggattag gtgtctttaa cgtcggtgcg gcgtacatcc
    50521 ataaccgcga tgtcctctcc gtcgccgggc cgtttatgcg ccgcctgatg ttactttccg
    50581 gcgaaacggt caatgtcgcg atccgtaacg gcaatgaagc ggtattaatt ggtcagttag
    50641 agtgtaaatc gatggtcagg atgtgtgcgc cactgggcag tcgtctgccg ctgcatgctt
    50701 ccggtgcggg caaagcgctg ctttatccgc tggcggaaga ggagttgatg agcatcattc
    50761 tgcaaaccgg tttgcagcag tttacgccaa ctacgcttgt ggatatgccc accttgctga
    50821 aggacctgga acaagcgcgt gaactgggct ataccgtaga taaagaagag catgttgtag
    50881 gtctgaattg catagcttca gcaatttacg atgatgtcgg tagagttgtt gccgctatct
    50941 ccatctccgg gccttcatca agactgacag aagatcgttt tgtcagtcag ggtgagctgg
    51001 tcagagacac cgcccgcgat atcagcacgg cgttgggact gaaagcacat ccatagtgtc
    51061 tgtcgcatcc cgctctgcgg agcgggtttt tttgacaaaa tttgaaagtt ggaaaatttt
    51121 tccaactaat agaggtagga ataaaatggc aaaaatgaga gccgttgacg cggcaatgta
    51181 tgtgctggag aaagaaggta tcaccaccgc cttcggcgtt ccgggagctg caattaatcc
    51241 gttctactca gcgatgcgta agcacggcgg tattcgtcac attctggcgc gtcatgtgga
    51301 aggtgtttcg cacatggcgg aaggttatac ccgcgcaacg gcagggaata tcggcgtatg
    51361 tctggggact tccggtcctg cgggcacgga catgatcacc gcgctctatt ccgcttctgc
    51421 tgattccatt cctattctgt gcattaccgg ccaggcaccg cgcgcccgtc tgcataaaga
    51481 agattttcag gccgtagata ttgaagcaat tgctaaaccg gtcagcaaaa tggcggttac
    51541 agttcgtgaa gcggcgctgg tgcctcgcgt gctgcaacag gcatttcacc tgatgcgttc
    51601 tggtcgtccg ggtccggtac tggtggattt accgttcgac gttcaggttg cggaaatcga
    51661 gtttgatcct gacatgtacg aaccgctgcc ggtctacaaa cctgctgcca gccgtatgca
    51721 gatcgaaaaa gctgtagaaa tgttaatcca ggccgaacgt ccggtgattg ttgccggggg
    51781 cggggtaatc aatgctgacg cagctgcact gttacaacag tttgctgaac tgaccagcgt
    51841 tccggtgatc ccaacgctga tgggctgggg ctgtatcccg gacgatcatg aactgatggc
    51901 cgggatggtc ggtctgcaaa ccgcgcatcg ttacggtaac gcaacgttgc tggcgtccga
    51961 catggtgttt ggtatcggta accgttttgc taaccgtcat accggttcgg tagagaaata
    52021 caccgaaggg cgcaaaatcg ttcatattga tattgagccg acgcaaattg gtcgcgtgct
    52081 gtgtccggat ctcggcattg tctctgatgc taaagcggcg ctgacactgc tggttgaagt
    52141 ggcgcaggag atgcaaaaag cgggtcgtct gccgtgtcgt aaagaatggg tcgccgactg
    52201 ccagcagcgt aaacgcactt tgctgcgcaa aacccacttc gacaacgtgc cggtgaaacc
    52261 gcagcgcgtg tatgaagaga tgaacaaagc ctttggtcgc gatgtttgtt atgtcaccac
    52321 tattggtctg tcacaaatcg ctgcggcaca aatgctgcat gtctttaaag accgccactg
    52381 gatcaactgt ggtcaggctg gtccgttagg ctggacgatt ccggcggcgc tggggggttg
    52441 tgccgctgat ccgaaacgca atgtggtggc gatttctggc gactttgact tccagttcct
    52501 gattgaagag ttagccgttg gcgcgcagtt caacattccg tacatccatg tgctggtgaa
    52561 taacgcctat ctcggcctga ttcgtcagtc acaacgcgct tttgacatgg actactgcgt
    52621 gcaactcgct ttcgagaata tcaactccag tgaagtgaat ggctacggtg ttgaccacgt
    52681 aaaagtagcg gaaggtttag gttgtaaagc tattcgggtc ttcaaaccgg aagatattgc
    52741 gccagccttt gaacaggcga aagccttaat ggcgcaatat cgggtaccgg tagtcgtgga
    52801 agttattctc gagcgtgtga ccaatatttc gatgggcagc gaactggata acgtcatgga
    52861 atttgaagat atcgccgata acgcagcgga tgcaccgact gaaacctgct tcatgcacta
    52921 tgaataaggg agataaatga tgttacgttt ctctgctaat ttatcgatgt tatttggtga
    52981 atatgatttt ctcgcccgtt ttgagaaagc tgcgcagtgt ggttttcgcg gcgttgaatt
    53041 tatgtttcct tatgactacg acattgaaga attaaaacag gtactggcga gtaataaact
    53101 cgaacatacg ctgcacaatt taccggcagg tgactgggcg gcgggcgagc gcggtattgc
    53161 ctgtattcct ggccgtgaag aagagtttcg ggatggcgta gcagcagcga ttcgt
//
LOCUS       DAETQL010000021        50034 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.021, whole genome shotgun sequence.
ACCESSION   DAETQL010000021 DAETQL010000000
VERSION     DAETQL010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 50034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50034
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.021"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..185
                     /locus_tag="K0W65_001314"
     CDS             <1..185
                     /locus_tag="K0W65_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3094642.1"
                     /translation="SRLKGKLHVISKRYTQRIERHNLNLRQHLARLVRKSLSFSKSVE
                     LHDKVIGHYLNIKHYQ"
     gene            178..1428
                     /locus_tag="K0W65_001315"
     CDS             178..1428
                     /locus_tag="K0W65_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBI3094643.1"
                     /translation="MNKLESLPEFELPEITDNELLVSVISDSVCLSTWKAALLGSEHK
                     RVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEY
                     FGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHR
                     MGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLA
                     ASKGIELVYVNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLN
                     FFAGLTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHI
                     GGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETH
                     GIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            1425..2390
                     /gene="yggF"
                     /locus_tag="K0W65_001316"
     CDS             1425..2390
                     /gene="yggF"
                     /locus_tag="K0W65_001316"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBI3094644.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2412..2921
                     /gene="fumE"
                     /locus_tag="K0W65_001317"
     CDS             2412..2921
                     /gene="fumE"
                     /locus_tag="K0W65_001317"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HBI3094645.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2918..3631
                     /locus_tag="K0W65_001318"
     CDS             2918..3631
                     /locus_tag="K0W65_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBI3094646.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3603..4280)
                     /locus_tag="K0W65_001319"
     CDS             complement(3603..4280)
                     /locus_tag="K0W65_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBI3094647.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4274..4951)
                     /locus_tag="K0W65_001320"
     CDS             complement(4274..4951)
                     /locus_tag="K0W65_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBI3094648.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4939..5322)
                     /locus_tag="K0W65_001321"
     CDS             complement(4939..5322)
                     /locus_tag="K0W65_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBI3094649.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5338..5646)
                     /locus_tag="K0W65_001322"
     CDS             complement(5338..5646)
                     /locus_tag="K0W65_001322"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094650.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5647..6225)
                     /locus_tag="K0W65_001323"
     CDS             complement(5647..6225)
                     /locus_tag="K0W65_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBI3094651.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6248..6679)
                     /locus_tag="K0W65_001324"
                     /pseudo
     CDS             complement(6248..6679)
                     /locus_tag="K0W65_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            7051..8070
                     /gene="epd"
                     /locus_tag="K0W65_001325"
     CDS             7051..8070
                     /gene="epd"
                     /locus_tag="K0W65_001325"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBI3094652.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            8120..9283
                     /gene="pgk"
                     /locus_tag="K0W65_001326"
     CDS             8120..9283
                     /gene="pgk"
                     /locus_tag="K0W65_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBI3094653.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9498..10577
                     /gene="fbaA"
                     /locus_tag="K0W65_001327"
     CDS             9498..10577
                     /gene="fbaA"
                     /locus_tag="K0W65_001327"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBI3094654.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10768..11628
                     /gene="mscS"
                     /locus_tag="K0W65_001328"
     CDS             10768..11628
                     /gene="mscS"
                     /locus_tag="K0W65_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBI3094655.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11767..12402
                     /gene="argO"
                     /locus_tag="K0W65_001329"
     CDS             11767..12402
                     /gene="argO"
                     /locus_tag="K0W65_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBI3094656.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12495..13235
                     /locus_tag="K0W65_001330"
     CDS             12495..13235
                     /locus_tag="K0W65_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBI3094657.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13402..14352
                     /locus_tag="K0W65_001331"
     CDS             13402..14352
                     /locus_tag="K0W65_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3094658.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14294..15772)
                     /gene="scpC"
                     /locus_tag="K0W65_001332"
                     /pseudo
     CDS             complement(14294..15772)
                     /gene="scpC"
                     /locus_tag="K0W65_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15796..16581)
                     /gene="scpB"
                     /locus_tag="K0W65_001333"
     CDS             complement(15796..16581)
                     /gene="scpB"
                     /locus_tag="K0W65_001333"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HBI3094659.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16592..17587)
                     /gene="meaB"
                     /locus_tag="K0W65_001334"
     CDS             complement(16592..17587)
                     /gene="meaB"
                     /locus_tag="K0W65_001334"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HBI3094660.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17580..19724)
                     /gene="scpA"
                     /locus_tag="K0W65_001335"
     CDS             complement(17580..19724)
                     /gene="scpA"
                     /locus_tag="K0W65_001335"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBI3094661.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19928..20821)
                     /gene="argP"
                     /locus_tag="K0W65_001336"
     CDS             complement(19928..20821)
                     /gene="argP"
                     /locus_tag="K0W65_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HBI3094662.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21204..21863
                     /gene="rpiA"
                     /locus_tag="K0W65_001337"
     CDS             21204..21863
                     /gene="rpiA"
                     /locus_tag="K0W65_001337"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBI3094663.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            22119..23351
                     /gene="serA"
                     /locus_tag="K0W65_001338"
     CDS             22119..23351
                     /gene="serA"
                     /locus_tag="K0W65_001338"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBI3094664.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23580..23639
                     /gene="ibsC"
                     /locus_tag="K0W65_001339"
     CDS             23580..23639
                     /gene="ibsC"
                     /locus_tag="K0W65_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBI3094665.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23972..24031
                     /locus_tag="K0W65_001340"
     CDS             23972..24031
                     /locus_tag="K0W65_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBI3094666.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(24132..24734)
                     /locus_tag="K0W65_001341"
     CDS             complement(24132..24734)
                     /locus_tag="K0W65_001341"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBI3094667.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24755..24938)
                     /gene="ssrS"
                     /locus_tag="K0W65_001342"
     ncRNA           complement(24755..24938)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="K0W65_001342"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24980..25309)
                     /gene="zapA"
                     /locus_tag="K0W65_001343"
     CDS             complement(24980..25309)
                     /gene="zapA"
                     /locus_tag="K0W65_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBI3094668.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25477..26055
                     /gene="ygfB"
                     /locus_tag="K0W65_001344"
     CDS             25477..26055
                     /gene="ygfB"
                     /locus_tag="K0W65_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBI3094669.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            26081..27406
                     /gene="pepP"
                     /locus_tag="K0W65_001345"
     CDS             26081..27406
                     /gene="pepP"
                     /locus_tag="K0W65_001345"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBI3094670.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27403..28581
                     /gene="ubiH"
                     /locus_tag="K0W65_001346"
                     /gene_synonym="visB"
     CDS             27403..28581
                     /gene="ubiH"
                     /locus_tag="K0W65_001346"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBI3094671.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28604..29806
                     /gene="ubiI"
                     /locus_tag="K0W65_001347"
                     /gene_synonym="visC"
     CDS             28604..29806
                     /gene="ubiI"
                     /locus_tag="K0W65_001347"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBI3094672.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29880..29963)
                     /locus_tag="K0W65_001348"
     CDS             complement(29880..29963)
                     /locus_tag="K0W65_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094673.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30254..31348
                     /gene="gcvT"
                     /locus_tag="K0W65_001349"
     CDS             30254..31348
                     /gene="gcvT"
                     /locus_tag="K0W65_001349"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBI3094674.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31372..31761
                     /gene="gcvH"
                     /locus_tag="K0W65_001350"
     CDS             31372..31761
                     /gene="gcvH"
                     /locus_tag="K0W65_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBI3094675.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31880..34753
                     /gene="gcvP"
                     /locus_tag="K0W65_001351"
     CDS             31880..34753
                     /gene="gcvP"
                     /locus_tag="K0W65_001351"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBI3094676.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            35020..35763
                     /locus_tag="K0W65_001352"
     CDS             35020..35763
                     /locus_tag="K0W65_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI3094677.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35820..37253)
                     /gene="bglA"
                     /locus_tag="K0W65_001353"
     CDS             complement(35820..37253)
                     /gene="bglA"
                     /locus_tag="K0W65_001353"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HBI3094678.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37298..37608
                     /locus_tag="K0W65_001354"
                     /pseudo
     CDS             37298..37608
                     /locus_tag="K0W65_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37772..38431
                     /locus_tag="K0W65_001355"
     CDS             37772..38431
                     /locus_tag="K0W65_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBI3094679.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38627..39607)
                     /gene="ygfZ"
                     /locus_tag="K0W65_001356"
     CDS             complement(38627..39607)
                     /gene="ygfZ"
                     /locus_tag="K0W65_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBI3094680.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39850..40116
                     /gene="sdhE"
                     /locus_tag="K0W65_001357"
     CDS             39850..40116
                     /gene="sdhE"
                     /locus_tag="K0W65_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBI3094681.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            40097..40504
                     /locus_tag="K0W65_001358"
     CDS             40097..40504
                     /locus_tag="K0W65_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HBI3094682.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40544..41065)
                     /gene="fldB"
                     /locus_tag="K0W65_001359"
     CDS             complement(40544..41065)
                     /gene="fldB"
                     /locus_tag="K0W65_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBI3094683.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            41177..42073
                     /gene="xerD"
                     /locus_tag="K0W65_001360"
     CDS             41177..42073
                     /gene="xerD"
                     /locus_tag="K0W65_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBI3094684.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            42098..42808
                     /gene="dsbC"
                     /locus_tag="K0W65_001361"
     CDS             42098..42808
                     /gene="dsbC"
                     /locus_tag="K0W65_001361"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBI3094685.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42814..44547
                     /gene="recJ"
                     /locus_tag="K0W65_001362"
     CDS             42814..44547
                     /gene="recJ"
                     /locus_tag="K0W65_001362"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBI3094686.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44638..45736
                     /gene="prfB"
                     /locus_tag="K0W65_001363"
     CDS             join(44638..44712,44714..45736)
                     /gene="prfB"
                     /locus_tag="K0W65_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBI3094687.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45746..47263
                     /gene="lysS"
                     /locus_tag="K0W65_001364"
     CDS             45746..47263
                     /gene="lysS"
                     /locus_tag="K0W65_001364"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBI3094688.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47306..47854)
                     /gene="idi"
                     /locus_tag="K0W65_001365"
     CDS             complement(47306..47854)
                     /gene="idi"
                     /locus_tag="K0W65_001365"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBI3094689.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47909..47980
                     /locus_tag="K0W65_001366"
     CDS             47909..47980
                     /locus_tag="K0W65_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094690.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47977..48102)
                     /locus_tag="K0W65_001367"
     CDS             complement(47977..48102)
                     /locus_tag="K0W65_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094691.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(48104..49552)
                     /gene="uacT"
                     /locus_tag="K0W65_001368"
     CDS             complement(48104..49552)
                     /gene="uacT"
                     /locus_tag="K0W65_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBI3094692.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12116 a        12498 c        13454 g        11966 t
ORIGIN      
        1 aatcacgcct gaagggaaag ctgcacgtta tcagcaagcg ttacactcag cgcattgagc
       61 gacataatct gaatctgaga caacatctgg caaggctggt acggaagtca ctgtcgttct
      121 caaaatcggt ggagctgcat gacaaggtca tcgggcatta tctgaacata aaacactatc
      181 aataagttgg agtcattacc cgaatttgaa ctgccagaaa ttaccgataa tgaactgtta
      241 gtgagtgtaa tttctgacag cgtctgttta tcgacctgga aagcggcgtt actcggtagt
      301 gaacataaac gcgtacccga cgatttagaa aatcatccgg tcattaccgg gcatgaatgt
      361 gccggggtta ttgtcgaagt gggtaaaaat ctcactggca aatataaaaa aggccagcgt
      421 tttgtattgc aaccggcgat ggggttacca agcggatatt cagcgggcta cagctacgaa
      481 tattttggcg gcaatgccac ttatatgatt attcccgaaa tcgccattaa tttgggctgc
      541 gtattaccgt atcacggctc ttattttgct gcggcgtcgc tggcagagcc tatgtgctgc
      601 attattggtg cttatcatgc caattatcac accacgcaat atgtttatga gcatcgcatg
      661 ggcgtcaaac ctggcggcaa tattgcactg ctggcgtgtg cgggtccgat gggcattggc
      721 gctatcgatt acgccattaa cggtggcata caaccgtcac gggtggtggt ggtcgatatc
      781 gacgacaaac gcctggcgca ggtgcagaag ctgctgccgg tggatctggc ggccagtaaa
      841 ggcattgagc tggtgtatgt gaataccaaa gggatgagcg atcctatcca gatgctgcgg
      901 gcgctgacag gagatgtcgg gttcgatgac atttttgttt atgcggcggt gcctgctgtc
      961 gttgagatgg ctgacgaatt actggcggaa gatggctgtc tgaacttctt tgccgggctg
     1021 acggataaaa acttcaaagt gccgtttaat ttctacaacg tccattacaa cagcacgcac
     1081 gtagtcggta catccggcgg ttcaacggac gacatgaaag aggcgattgc ccttagcgcc
     1141 acaggacagt tacagccgtc gtttatggtg acccatattg gtggcctgga tgcggtgcca
     1201 gaaaccgtgc tcaatctgcc ggatatccct ggcggtaaaa aactcattta taacggcgtg
     1261 accatgccgc tcactgccat tgccgatttt gccgaaaaag gcaaaaccga tccgctgttt
     1321 aaagagttgg cgcggctggt tgaggaaacg cacggcatct ggaatgaaca ggccgagaaa
     1381 tatctgctgg cacaatttgg cgttgatatc ggggaggccg cgcaatgatg tccctggcgt
     1441 ggccattatt tcgcgttacg gaacaggcag cgttggctgc ctggccgcaa accggatgtg
     1501 gcgacaaaaa taaaattgat ggcctggcgg tcaccgcgat gcgccaggca ttaaacgacg
     1561 tcgcttttcg tggacgagtg gttatcggcg aaggagaaat tgaccatgcg ccgatgctgt
     1621 ggattggcga agaggtgggc aaaggcgatg ggccagaagt cgatatcgcg gttgatccca
     1681 ttgaaggcac gcggatggtg gcgatggggc agagcaatgc gctggcggtc atggctttcg
     1741 ctcctcgaga tagcctgtta catgcgcccg atatgtatat gaaaaagctg gttgttaatc
     1801 gactggctgc cggggcgatt gatctgtctt tgcctctggc ggacaacttg cgtaacgtgg
     1861 cgcgggcgtt aggtaaaccg ctggacaagc tgcgcatggt tacgctggat aaaccgcgtc
     1921 tgagtgccgc gattgaagaa gcaacacagc tgggcgtgaa ggtttttgcc ctgccggatg
     1981 gcgatgtcgc cgccagcgtg ctgacctgct ggcaggataa tccttacgat gtgatgtaca
     2041 ccatcggcgg tgcgccagaa ggcgtgattt ctgcctgtgc cgttaaagcg ttaggcgggg
     2101 atatgcaggc cgagctgatt gacttttgtc aggctaaagg ggattacacg gaaaatcggc
     2161 agattgcaga gcaggagcgc aagcgttgta aggcaatggg cgttgacgtt aaccgtgtgt
     2221 actcgctcga tgaactggtg agggggaacg atatcctctt tagcgctacg ggcgtgacgg
     2281 gcggcgagtt ggtgaacggt atccaacaga cggcgaatgg ggtgcggacg cagacattac
     2341 tgatcggcgg cgcggaccaa acgtgtaata taatagactc cctgcattga tggtgatcta
     2401 tcggaagacc aatggcgacg ctgacagaag atgatgtgct tgagcaactg gatgcacagg
     2461 acaatttatt ttcatttatg aaaactgcgc attctatttt gctccagggg atacgccagt
     2521 ttctgccgtc gctgtttgtc gataacgatg aagagatcgt cgaatatgca gtgaagccgt
     2581 tactcgccca aagcggcccg cttgacgata ttgatgttgc gctgcgtttg atttatgcgc
     2641 tggggaaaat ggataaatgg ctgtatgccg atatcacgca cttttcccag tactggcatt
     2701 acctgaacga acaggatgaa acgcccggat ttgccgatga tataacctgg gattttatca
     2761 gcaatgtcaa cagtataact cgcaatgcga cgctctacga tgcgttaaaa gcgatgaagt
     2821 tcgccgattt cgccgtctgg tcagaggcgc gttttagcgg aatggtcaaa acggcgctga
     2881 cgctggcagt aacgaccacc ttaaaggaat taacgccgtg aaaattgaat taacggtgaa
     2941 tgggctgaac gtgcaagcgc agtaccatga tgaagagata gaacgcgtgc ataaactgtt
     3001 gctgcgtatg ctggcggcct tacagacggt aaatccgcag cggcgtacgg tggtttttct
     3061 ttgcgctccg ccggggacgg gtaaatctac cctaaccacc ttctgggaat atctcgcgca
     3121 acaagacccg gagctgcccg ctattcaggc gctcccgatg gatggttttc accattacaa
     3181 tagctggctg gacgcgcatc aattgcgccc cttcaaaggc gcaccagaga catttgacgt
     3241 tgcgaaactg gcggaaaatc tgcgccaggt ggtggaaggg gattgtacgt ggccgcagta
     3301 cgatcgacaa aagcatgatc ctgttgaaga tgcgttgcac gttaccgcac cactcgtcat
     3361 cgtcgaagga aactggttgt tgctggacga cgagaagtgg tgccagcttg cgcaattttg
     3421 tgatttctcg atctttatca acgcgccagc tgctgcgttg cgggagcgtc tggtggggcg
     3481 caagctggcg ggaggggtat cgctggcaga tgcagaagcc ttttatgacc gtaccgacgg
     3541 gccgaatgtt cgccgggggc tggaagagag tctgcctgca aatttgacct taatgatgac
     3601 cgctacgggt gaatatcgtc tggtggatta acgttcctga ttacaccatc ttccagccgc
     3661 accacacgag agaaatgacg ccgggtaaat gcggcgtcat gactgattgc taccacgctc
     3721 gtgcctcgct ggcggcactt ctccagccag ctttcaaaca cgctcaacca attttcgtca
     3781 aaatcccgac tcggctcgtc cagcagtaat agtggcggag agagtgcttc caggcaggcg
     3841 acggcgacca tccgtcgctg cgcattatgc agatccaacg ggtgcgagtt ggcagtgtct
     3901 gctaactgac aacattgcag ggcagcatgg gtacgttgag tgatttcatc cgccgggcat
     3961 ttttgcagtt tcaaaccaaa tgcgatctcg tcagcgacgg tgctgtgaaa aagttggttt
     4021 tccgcttcct gaaacagtac gccaaccttt gccgcgcgct ggcggttttt caggtttttc
     4081 atcgcctgtt gttgcagcat aaccgtaccg gctgtgggcg taagaagacc tgccattact
     4141 cgtaacagcg tcgatttccc tgcgccattg tcaccggtca gcgccaacca ttcaccttgt
     4201 ttgagttgca gagaaatgtc gcaaagacaa tccgttgccg cgccaggcca gcgataagat
     4261 atcttattta acgttaacat agtggctgga gcgctccgtt ttgcaattgc cacgcttgct
     4321 ggcagtagtt caggaaaggt gtgcgatgac gttcaaaaag aataatcagc gaaccacgtt
     4381 caaacgccca gtgttgtaag cgctgcaaca gcatttcgct ggcctgcggt gtcaggcggc
     4441 taaaggcttc atcaagaatt aagagctttg gctgcatcgc gatggcgcag gcgatgatca
     4501 cgcgctgtgt ctctccaccg gagagtgttg ccgggtgacg atggcgcaac ggctggcact
     4561 cggtcagagt cagggcggca tcaatccgcg ccataatctc tgcttcagca agacacagat
     4621 tttctggccc aaaggcaact tcttcttcca cgctaaaggt acagccggag agctgcaaat
     4681 agggcgattg ctggacgagc tggatggtgg cggattgttc attaagcggg agatgcccaa
     4741 tgggcgtacc gaggagcgtc cccgtgcctg ttatctctct tggcagaaag tccggatacc
     4801 agcccgccat cagctgtgcc agcgtacttt tgccactgcc gttgtcacca aagatggcga
     4861 ccatccccgg tttgacatag tgaaagtcat aacagaacgg aggatgcgtt gagtgtgtcg
     4921 ggcaataacg gaactgttct aacgtaacca tatccacgct ccgaattctg tcagcatcag
     4981 caagatcatg gtgtaacgcg ctacgcgttg tagggtgcta tcggctggtg cccacaacgt
     5041 tgttcgtcgg ttgtggatcc gaaatgcccg catatccagc gccgcaccac ggacagccag
     5101 atcgttaagc gcattgtgtg ttagcggaat aattaacgcg ggcatagcac gtaaacgctg
     5161 ataccagccc tcatccagcg gcacgccgcg ggcacgctgc gcttcatgaa taatcgctaa
     5221 ttgccgtttt aactgctcaa cgaccaataa cggcccggca aacagataag cgacgcctgg
     5281 cggcaggcga gaagcaaaca gagcgcgaat aaaacattgc acggggacgt actgcattca
     5341 caactgtgag gttgaaacaa tcgccagaat cagcaaccag agcgtaatag catgcgacca
     5401 ccgttctggc gagcgtggcg tgccgcttag ccattctggt agccagccac cgtgtaccag
     5461 ccacagcccg gctcccagcg agaacatcag ccagacgaca tattttgccc gccgccgcgt
     5521 ggcctttagc gcaataaggc agaaaaaggt cgccgcgcta tagatcggca aaatggtctg
     5581 cgcgggcagg ataagcgtgg tgcaggccgc taatgcccac agtgttaatg aggtaaatgg
     5641 atgcatttag cgcacggcag ccattgccgg aaaatggcgc gtggtgcgaa tcggcagctg
     5701 gcgcagtaaa agccagacaa tcaccgctgt aagaatttta tcgaccaggt tcgcgcctat
     5761 caccgtaatc gccaccgact ccaccaggtt ttgccccatc gagtgcatcc aggcaacgaa
     5821 caaatcggct ccgctaccgg tgacaccgcc gaacagcgca gtacgcagcg gtacagcgac
     5881 gaccgttacc gcaagcgtaa taatcacgcc gctgacaacg acttttggca gggtgcgaaa
     5941 ccaacccgct cgcgccagcc aaccggagac caggccgatg accatcgcga cgggtgcaaa
     6001 tgcggcggcg atcgggtcgg tcagcaatcc ccagagtaaa ttggtcagta agccggtcag
     6061 cattccgatc accggaccaa gcagtaccgc gctaataagt gtgccgatgg agtcgagaaa
     6121 tataggtaat ttgaccatgc tggccagttg cccgccgatc atattgatgg cgatagatat
     6181 aacgatcaac accagtgcct gactggaaaa gtggctacgc gccataagaa aacctctttg
     6241 aaagtagtta tttaacgggg gaaatatgca actcgtcgta accagaaagc gcgcaaggtt
     6301 ggcggctaaa cgccagttcc tcaaggctac cgatcaccag ttcaagcgtg gtccgcacgg
     6361 tacagccggg catcatatga ccgccaagca tcgtgccgtg tggatcggaa acgcagagat
     6421 gaagatgctc accactctgt tccagcgtgc cgtttagtga gatcacttcg aatgtaccgt
     6481 tcaggagcgt ggtgccttct tgcccggcat agcgcaaggc gacgtcagtc aggctaccgg
     6541 tacagcctgc aatctaggcg gcatgaagtt gatgttgctg tgcaaaagcg tgtagctggg
     6601 aaaaaacttc ctgcccgggg aggaggcgca gtgcgtaaaa gcgtgcggag gaagaatatg
     6661 agtagggcaa tgtcatcatt ggagtactcc gaaactcgga cgtattgcgg ataatatcga
     6721 cgctgcatca gtcatattct gatataagtc agggtttgtg acggcacaac gaaaagattt
     6781 tgctacgctt attcgtgatt ctgcaaagtg gaataaattc cacaggggcg gggctgaaaa
     6841 cgtgacgtaa gtcagataat tgctttcttt gactggacaa tcattccttt tattccacgt
     6901 ttcgcttatc ctagctgaag cgtttcagtc gattaaatgt tcgacaatta accaatcagt
     6961 cgcagtttgc gacaggtaag gtttccccgg acgatttgct ggattactct gtctggcaaa
     7021 tttgctcagg gaaaaccttg caggagatct atgaccgtac gcgtagcgat aaatggcttc
     7081 ggtcgcatcg ggcgtaatgt ggttcgtgct ttgtatgaat ccggacgccg ggcggaaatt
     7141 accgtggtgg caatcaacga actggcggat gctgcgggca tggcgcattt gttgaaatat
     7201 gacaccagcc atggccgttt tgcatgggaa gtacgacagg aacgcgatca actttttgtt
     7261 ggtgatgacg ccatccgcgt attgcatgaa cgttcactgc aatcgctccc ctggcgtgaa
     7321 cttggcgttg atgtagtcct cgactgcacc ggcgtatatg gctcccgcga gcatggcgaa
     7381 gcgcatattg ccgccggggc caaaaaagtg ctcttttcac atcctggcag taacgatctc
     7441 gacgcgaccg ttgtttacgg cgtcaatcag gatcaacttc gtgcggaaca ccgcatcgtt
     7501 tctaacgctt cctgtaccac gaattgcata attcccgtca tcaaattgtt agatgatgcg
     7561 tacggtattg agtccggcac tgtgaccaca attcactccg ccatgcacga tcaacaggtt
     7621 attgatgcat accatcctga cctgcgtcgc acccgggcag ccagccagtc gatcattccg
     7681 gtcgatacta aactggccgc tggtatcaca cgattttttc cgcaatttaa cgatcgcttt
     7741 gaagcgattg cggtacgtgt gccaaccata aatgtgacgg caatcgattt aagcgtgacg
     7801 gtgaagaaac ctgtaaaagc caatgaagtc aacctgttgc tgcaaaaagc agcacaaggt
     7861 gcatttcatg gtatagttga ctatacggaa ttgccgttgg tctctgtaga ttttaaccac
     7921 gatccgcaca gtgccattgt cgatggcacc caaacccggg tcagtggcgc acacctgatc
     7981 aaaacgttgg tctggtgcga taacgaatgg ggctttgcta accgaatgct cgacacgacg
     8041 ttagctatgg ctactgttgc tttcaggtaa gacgcaagca gcgtctgcaa aacttttaga
     8101 atcaacgaga ggattcacca tgtctgtaat taagatgacc gatctggatc ttgctgggaa
     8161 acgtgtattt atccgtgcgg atctgaacgt accagtaaaa gacgggaaag taaccagcga
     8221 cgcgcgtatc cgtgcttctc tgccgaccat cgaactggcc ctgaaacaag gcgcaaaagt
     8281 gatggtaact tcccacctgg gtcgtcctac cgaaggcgag tacaacgaag aattctctct
     8341 gctgccggtt gttaactacc tgaaagacaa actgtctaac ccggttcgtc tggttaaaga
     8401 ttacctcgac ggcgttgacg ttgctgaagg tgaactggtt gttctggaaa acgttcgctt
     8461 caacaaaggc gagaagaaag acgacgaaac cctgtccaaa aaatacgctg cactgtgtga
     8521 cgtgttcgta atggacgcat tcggtactgc tcaccgcgcg caggcttcta ctcacggtat
     8581 cggtaaattc gctgacgttg cgtgcgcagg cccgctgctg gcagctgaac tggacgcgct
     8641 gggtaaagca ctgaaagaac ctgctcgccc gatggtggct atcgttggtg gttctaaagt
     8701 atctaccaaa ctgaccgttc tggactccct gtctaaaatc gctgaccagc tgattgttgg
     8761 tggtggtatc gctaacacct ttatcgcggc acaaggccac gatgtgggta aatccctgta
     8821 cgaagctgac ctggttgacg aagctaaacg tctgctgacc acctgcaaca tcccggttcc
     8881 gtctgatgtt cgcgtagcaa ccgagttctc tgaaactgca ccggctaccc tgaaatctgt
     8941 taacgatgtg aaagctgacg agcagatcct ggatatcggt gatgcttccg ctcaggaact
     9001 ggctgaaatc ctgaagaatg cgaaaaccat tctgtggaac ggtccggttg gcgtgttcga
     9061 attcccgaac ttccgcaaag gtactgaaat cgtggctaac gctatcgcag acagcgaagc
     9121 gttctccatc gctggcggcg gcgacactct ggcagcaatc gacctgttcg gcattgctga
     9181 caaaatctcc tacatctcca ctggcggcgg cgcattcctc gaattcgtgg aaggtaaagt
     9241 actgcctgca gtagcgatgc tcgaagagcg cgctaagaag taaaaaatca cagggcaggg
     9301 aaacctgcac ttgtttcagc gcgcttttag agcacgcatt ttttcatact ctaaataatt
     9361 cgagttgcag gaaggcgaca agagagtgaa tccccaggag cttacataag taagtgactg
     9421 gggtgagcga acgcagacgc agcacatgca acttgaagta ttatgagtat aaggcccgac
     9481 gatacaggac aagagacatg tctaagattt ttgatttcgt aaaacctggc gtaatcactg
     9541 gtgatgacgt acagaaagtt ttccaggtag caaaagaaaa caacttcgca ctgccagcag
     9601 taaactgcgt cggtactgac tccatcaacg ccgtactgga aactgctgct aaagttaaag
     9661 cgccggttat cgttcagttc tccaacggtg gtgcttcctt tatcgctggt aaaggcgtga
     9721 aatctgacgt tccgcagggt gctgctatcc tgggcgcgat ctctggtgcg catcacgttc
     9781 accagatggc tgaacattat ggtgttccgg ttatcctgca cactgaccac tgcgcgaaga
     9841 aactgctgcc gtggatcgac ggtctgctgg acgcgggtga aaaacacttc gcagctaccg
     9901 gtaagccgct gttctcttct cacatgatcg acctgtctga agaatctctg caagagaaca
     9961 tcgaaatctg ctctaaatac ctggagcgta tgtccaaaat cggcatgact ctggaaatcg
    10021 aactgggttg caccggtggt gaagaagacg gcgtggacaa cagccacatg gacgcttctg
    10081 cactgtacac ccagccggaa gacgttgatt acgcatacac cgagctgagc aaaatcagcc
    10141 cgcgtttcac catcgctgcg tccttcggta acgtacacgg tgtttacaag ccgggtaacg
    10201 tggttctgac tccgaccatc ctgcgtgatt ctcaggaata tgtttctaag aaacacaacc
    10261 tgccgcacaa cagcctgaac ttcgtattcc acggtggttc cggttctact gctcaggaaa
    10321 tcaaagactc cgtaagctac ggcgtagtga aaatgaacat cgataccgat acccaatggg
    10381 caacctggga aggcgttctg aactactaca aagcgaacga agcttatctg cagggtcagc
    10441 tgggtaaccc gaaaggcgaa gatcagccga acaagaaata ctacgatccg cgcgtatggc
    10501 tgcgtgccgg tcagacttcg atgatcgctc gtctggaaaa agcattccag gaactgaacg
    10561 cgatcgacgt tctgtaagtc attcctttct gcttatctca aggcccgctc tgcgggtctt
    10621 tttttcgcca aaagcaaggt gattcagatg agaaatctgt gatctatttg gcaaaattat
    10681 gctttattgt ttacccttgt cagactgccc gtcataaggc ggcggagtgt atttctccat
    10741 tttgagtcag ttgaaaagga atattgaatg gaagatttga atgttgtcga tagcataaac
    10801 ggcgcgggaa gctggctggt agctaaccag gcgctgctgc taagttatgc agtaaacatc
    10861 gtggcggcac tcgcgatcat catcgttggt ttgattatcg cgcggatgat ttccaacgcg
    10921 gtgaatcgcc tgatgatctc ccgtaaaatc gatgccactg ttgctgattt tctttctgca
    10981 ttagtccgtt acggtattat cgcctttacg ctaatcgctg ccctgggacg cgtgggtgta
    11041 caaacagcgt cagtcattgc tgtactcggt gccgcaggct tagctgttgg tctggctttg
    11101 caggggtcac tttctaacct ggccgctggc gtgttacttg tcatgttccg cccgttccgt
    11161 gccggagaat atgttgacct cggcggcgta gccggtactg tgctgagtgt gcagattttc
    11221 tccaccacca tgcgtactgc agacggtaaa attatcgtta ttccgaacgg taaaattatt
    11281 gccggaaata ttattaactt ctcccgcgag ccagttcgcc gtaacgaatt tattattggc
    11341 gtggcgtatg attccgatat cgatcaggtt aagcagatcc tgaccaatat tatccagtct
    11401 gaagatcgca ttttgaaaga tcgcgaaatg actgtgcgcc tgaacgaact tggtgcatcg
    11461 tcgattaatt tcgtggtccg cgtctggagc aacagcggcg atctgcaaaa cgtgtactgg
    11521 gatgtgctgg agcgtattaa acgtgaattt gatgccgccg gtatcagctt cccgtacccg
    11581 caaatggatg tgaactttaa gcgggtgaaa gaagacaaag ctgcgtaatc aacgctgagg
    11641 ccagataata ctctctggcc tctctcttat tagttattct gattgccaat taatattatc
    11701 aatttccgct aataacaatc ccgcgatata gtctctgcat cagatactta attcggaata
    11761 tccaacgtgt tttcttatta ctttcaaggt cttgctcttg gggcagctat gattctgccg
    11821 ctcggcccac aaaatgcttt tgtgatgaat cagggcattc gtcgtcagta ccacattatg
    11881 attgccttac tttgcgctat cagcgatttg gtcctgattt gcgcggggat ttttggtggt
    11941 agcgcgttat tgatgcagtc gccgtggttg ctagcgctgg tcacctgggg cggcgtagtc
    12001 ttcttgctct ggtatggttt tggcgctttt aaaacagcga tgagtagtaa tattgagtta
    12061 gctagtgccg aagtcctgaa gcaaggcaga tggaaaatta tcgccaccat gttggcagtg
    12121 acctggctga atccgcatgt ttacctggat acttttgttg tactgggcag ccttggcggg
    12181 cagcttgacg tcgaaccaaa acgttggttt gcgctcggga caattagcgc ctctttcctg
    12241 tggttctttg gtctggctat tctcgcagcc tggctggcac cgcgcctgcg cacggcaaaa
    12301 tcacagcgca ttatcaatct ggtagtggga tgtgtcatgt ggtttatagc cttgcagctg
    12361 gcgagagacg gtattgctca tgcacaagcc ttgttcagtt aggcgctatc tgatggaaaa
    12421 ataaaacaga ggcgctaagc ttgcctccag aggtcctgaa ttttgggcaa gtcaattaac
    12481 acggagagac taacgtgaag ttcaaagtta tcgccctggc ggcattaatg ggtattagcg
    12541 ggatggcagc gcaggctaac gaattgccgg atggaccgca tattgtcacc tccggtacgg
    12601 caagtgtgga tgcggtgcca gacattgcca ctcttgcgat tgaagttaac gtggccgcga
    12661 aggatgccgc tacagccaag aaacaggcag atgagcgcgt cgcacaatac atttccttcc
    12721 ttgaactcaa tcagatcgcg aaaaaagata tcagctcagc gaatttacgc acccagccag
    12781 attatgacta tcaggatggt aaaagtatcc tgaaaggcta ccgagctgtg agaacggtgg
    12841 aagtcacgct ccgtcagtta gacaaactga attccttgct ggatggcgcg ctgaaggcgg
    12901 gtcttaacga aattcgttct gtgtcgctgg gcgtggcgca gccggatgcc tataaagaca
    12961 aagcgcgtaa ggcagcgatt gataacgcga ttcatcaggc gcaggaactg gcgaacggct
    13021 ttcatcgtaa actggggccg gtatatagcg tgcgctacca tgtttccaac tatcagccca
    13081 gcccaatggt gcggatgatg aaagccgatg ccgcgccggt gtctgcccag gaaacttacg
    13141 agcaggccgc tattcagttt gatgatcagg tcgatgtggt cttccagtta gaacctgtgg
    13201 atcaacaacc cgctaaaaca cctgcagcac aataatactt gtagcccgac gtattcgtcg
    13261 ggctaatgcc tttacccgat attgcctcct ttttctgtaa ttttctgtaa aaccatgacg
    13321 taatcaccta tctttgctcg cggtttcatc aaggataggc tgactgaaac ggttaagcgg
    13381 acaaggatgc ggggaggggg gatggacatt tttatttcga aaaagatgcg caatttcatt
    13441 ttattagcgc aaaccaataa tattgcccgg gcggcagaaa aaatccatat gacggcttcg
    13501 ccatttggta aaagcattgc cgcgctggaa gaacaaattg gctatacgct atttacccgc
    13561 aaagataata atatcagcct caataaagcc ggtcaggaac tgtatcaaaa actgttcccg
    13621 gtttatcaac gactttctgc gattgataat gaaatccata actcagggcg tcgttcacga
    13681 gaaattgtga ttggcataga taatacttat ccgacaatta ttttcgatca gttgattagt
    13741 ctcggagata agtatgaagg cgtgaccgcc cagccagttg agttcagcga aaacggcgtt
    13801 atcgataatc tttttgaccg tcagttagat tttattatct ccccacagca tgtatctgca
    13861 cgagttcagg aactggaaaa cctgactatc agtgaattac caccgctgcg attgggcttt
    13921 ctcgtttctc gccgttatga agagcgtcaa gagcaagagc tgcttcagga gctgccctgg
    13981 ctacaaatgc gctttcaaaa ccgggctaac ttcgaagcaa tgattgatgc gaatatgcga
    14041 ccttgtggga tcaatcccac cattatttat cgtccctaca gttttatggc caaaatcagc
    14101 gccgtggagc gcggacattt tctgacggtg atcccgcatt ttgcctggcg actggtgaat
    14161 ccggcaacgc tgaaatattt tgatgcgccg cacaggccga tgtatatgca ggaatatctt
    14221 tattcaatca gaaatcatcg ttataccgcc acgatcttca gcatattgct gaagatcgtg
    14281 acgggacaaa ccattaaccc agcatcgagc cggttgcaat taaattacgg tgtaagtcga
    14341 agacgtggct aagatcgtgg tgaatatgtc cgccgggcgc attttccaga tagcgatgca
    14401 gataatcccg atacatagga tgtgcacaat tatcaatgat agtgcgggcg cgttgaagcg
    14461 gggaaagacc gcgcagatcg gcgatccctt gttcggtgat gatcactttg acgctgtgtt
    14521 cgctgtgatc aacatggctg cacattggca cgacggttga gatcttgcct tctttagcaa
    14581 tcgacggagc cataaagatc gacagatacg cgttgcgttc aaaatcaccg ctgccgccga
    14641 tgccgttcat cagatcgacc ccggctacgt gcgttgaatt tgcatgcccg taaatatcaa
    14701 actccaggcc gacgttcaga gcgatgacgc ccagacgacg gatgatttcc gggttattgg
    14761 aaatctcctg cggacgcaac acaatgcggc tggcaaagta atccatattg tcgtaaatct
    14821 tgcgcaggga atcggccgag attgtcaggc tggaggcgct ggccccgctg attttgccgg
    14881 tttccagtaa atgcaccacc gattcctgta gcacttccga atacatcatg aacggaggaa
    14941 tttccgggtt ttcccccaga cgtgccatta ccgcattatt gatattgccc acgccacttt
    15001 gcagcggcag aaattccggc ggaatacgcc catgcgccat ttcctgcaat aagaacgtga
    15061 ccacgttatc ggcaatctgc tggcacatgg gattttgctt atccagcata ttaccggcgt
    15121 cgggcaagtt ggtttccacg acggcgacaa tctttttcgg atcgatttgc acatagcggg
    15181 taccgacgcg atccattgca tggaagatcg acacgctatt gcgccgcggt ggcgcgccag
    15241 gaatcacaat atctgccagt tctgcaacgc gcggatcgtg atagtgatta agttcaatga
    15301 tcactttctt cgctcgcagc agctaggtcg gcgcattacc gatcccgctg gttaaccaga
    15361 ctcgaccatc cggtgccagt gccgatgctt caatgacggc aacatcaatg tcgccgaaga
    15421 aaccgtaatt gaccatttgc gccacttcgc tcaaatgcag gtcaacgaaa ctcaccgcgc
    15481 cctgattgat ctttttacgt aaactggacg atgtttgata tggcgcacgc caggaaacag
    15541 catcggcgtc agaaagtaca tcgtcagcgg cggcgctgat tgacgccccc gtcagcaggc
    15601 gaatttgata cggctttttg gcctcatgct gttcgttagc tctgcgggca atcgcggcgg
    15661 gtagggcttt cggcgaaccc gctggggtaa agccgctaaa tgccaccatg tcgttatgct
    15721 ggataatttc tgccgcttca tcggcggtca tccttgtcca ctgagtttcc atttttactc
    15781 cttcgttcgc agggattaat gaccaacgaa attaggttta cgtttttcga ggaaagcgtt
    15841 catcccttcc tggtaatctt cgctgtcata aaccgcgcgg cgcatcccct gaatacgttc
    15901 aaattcatcg gagttcatgg tgtgtgcttc gcccagtaca cgcagctctt ctttgataac
    15961 ggcaatggct aacggcgctt tctcagagat gtggtgcgcc atttgtaagg tgaaatcttc
    16021 cagttcttcc acttccacaa catggttgag gatgccgaca gccagcgcgc gctgggcggt
    16081 gattggcgaa gcggtaaaaa tcagctcttt gacaatgtgg aagcccgcgt cgcgggtcag
    16141 gttgtgaatg ccgaccaggt tatacgggac gccgaggttt acaggcgtca ttgagaaggt
    16201 tgaggtactg gcggcgatga tcagatcgga actcatgatc atttcaaatg cgccacccca
    16261 aacactacct tccaccatcg aaatgatcgg tttcgggaat ttttggatca tgcgggtgat
    16321 ttgacgcaat ggatcatcat aggagagcgg atcgcgaccg ccagacggca gttcgtgaat
    16381 atcgtgacct gcggagaaga ctttggatcc acttggcgcc cgcaaaatga tacagcgaat
    16441 ttccggccgg ttgagatcgc ttaacgcctg cataagatca tcaataaaga ctttacttaa
    16501 ggcattaagt tttcgtccat agttaaactc aatgaccgcc actttgttga tagtggcaac
    16561 gttcacatac tgataagaca taaaaattcc tttaatcaaa atattgcgtc tggataaatt
    16621 cactgagctg ccgcaggccg gtgcgcggtg agagcgtatt gtttttgacc gctaaaagcg
    16681 tctggcggta atagcgatcg aaatcttcat tcgcgaacag gtgattcagt acttcttctt
    16741 cggtctgctt acgcagccat tccaccgatt gttgttgccg cacttgttgt aaacgaccac
    16801 tggcagttag cgcggttttg aagtcgatga tggcgtgcca gatctcatcg attccacgtt
    16861 tttccagtgc gctacaagtc agaccccgtg gctgccattc gtcgtattta cgtcgcagaa
    16921 tatgcagggc actctcgtac atatgccggg caatggcgac attggtatgg ttatcgccat
    16981 cgtctttgtt gataacgagc agatcagcca cttccatcag ccctttttta atgccctgca
    17041 gatcatcgcc gccaccggca atttgcaacg agataaaaca gtccaccatg cgggcgactt
    17101 ccgtttccga ctgcgcgatg ccaaccgttt cgacaatcac tacgtcataa cccgccgctt
    17161 cgcataacag cattaattcc cgtgctcgct gactggcacc gcccagatga ccggaggagg
    17221 gtaccgggcg aataaacgcc gcctcggcac gcgccaggtc attcatgcgg gttttatccc
    17281 cgagaatgct accgccagtg accgggctgc tgggatcgac cgcaataaca gcaaccttta
    17341 atccctcacg aatcaacaac atgccaaagg cttcaagaaa ggtacttttc cccgcgccgg
    17401 gtgtgccggt aacgcccagt cgcagggcgt taccacagaa cggcataata gcgtcaagca
    17461 gctgtgtact tagcgcctga tgacgtgggt gacggctttc caccagcgtc atggcttggg
    17521 cgagcgtggc acgctcaccc tgacgtaagc ggcgaatact ttctgccagc gtggcttcat
    17581 taatcatgat gctggcttat cagattcagt acgtcgcgca cactgtcgag cataggtgta
    17641 cctggaccat aaatcgccgc cacgccgcgc tcttgcagga aggcgtaatc ctgcggcgga
    17701 atgacgccac ccgcgaccac gcagatatct tcgcgtcccc attttttcag cgcttcgacc
    17761 agttccggga tcagcgtttt atgaccggca gccagtgagg atgcgcccac tacgtgaacg
    17821 tcgttttcta cggctaggcg ggcgatctct tcaggtgtag agaacatcgg gcttaaatct
    17881 acgtcgaaac cgagatcgga ataggcgctg gcgatcactt tcgcgccgcg atcgtgtcca
    17941 tcctggccca tcttagcgat cagaatgcgc gggcgacgac cattgtcggc aaggaactgc
    18001 tccgtttgcg caacaatggc atcgaactcg gaggccgatt tctcagactg atgatagctt
    18061 tgcgcaatca cgccggtaac acactggctt ggcaccagat aacggtcgaa agcgacttcc
    18121 agcgcatcgg aaatttcacc cagggtggcg cgaacgcgag cggcattaac agcggcagcc
    18181 agcaggtttt cgttatgctg tgcggcgtga gtcagggcgt tcaacgcggc ggttacggcg
    18241 gcatcatcac gggtggcgcg aatgcgttcc agcgaagcaa tttgctcgtt acgcaccatc
    18301 acgttgtcga tctcaagtac atcggtttcg tcttcgtgat ccagtttgta cttgttgaca
    18361 ccaacgatga cacgcttgcc ctggtcgatc agcgactgtt cgcgcgctga ggcctcttcg
    18421 atcattcgtt ttggcagacc tgcttcgatc gctttcgcca tgccaccggc ttcgtcgatc
    18481 tgttggataa tagctctggc ttgtttgacg atttgatcgg tcagcgactc aatgtaatag
    18541 gatccggcca gtggatcgac ggtgtggcag agttctgatt cttcctggat gatgatctgg
    18601 gtgttgcggg caatgcgtgc tgagaaatcg gtaggcaaac caagcgcttc gtcaaaggcg
    18661 ttggtatgca gtgactgagt accgcccagc gtcgcagcca gtgcttcaat ggtggtgcgg
    18721 ataacgttgt tatacggatc ctgttcagtc aggctccagc ctgaggtctg gcagtgggta
    18781 cgcagcgcca gtgatttcgg atcctgtgcg ccaaatccac tgaccgcttc gctccataaa
    18841 taacgtgccg cacgcaacat ggcgacgttc ataaacagat ccatgccgat accgaagaat
    18901 aacgacaggc gaggagcgaa gtcatcaatt ttcagtccgg cagagattgc tgctttgatg
    18961 tactcaatcc catcggcgag cgtaaatgct acctgctgca cgcagttggc acccgcttcc
    19021 cccatgtggt aaccgctgat actgatggta ttaaatcgcg gcatgttgcc ggaacaccag
    19081 gcgatgatgt cggcgataat gcgcattgac ggttttggtg ggtaaatata ggtgttgcgg
    19141 cagaggtact ctttgagaat atcgttttga atggtgccgg tcagtttatc aggtgtaaca
    19201 ccttgctctt ctgcggcgac gatataaaac gccagtactg gtagcactgc gccattcatg
    19261 gtcatcgaaa ccgacatttt atccagcggg atctggtcga acaggacttt catatcttcc
    19321 acggtgtcga tagcgacgcc cgctttgccg acgtcacccg ccacgcgtgg gttatcggag
    19381 tcgtagccac ggtgggtggc aaggtcaaac gcaacggaaa gacctttttg cccggcggcc
    19441 aggttacggc gataaaaagc gttggactct tttgctgttg aaaaaccagc atactgacgg
    19501 atggtccacg gttgggcggt atacatagtg gcacgcgggc cacgaacgta gggcggcaaa
    19561 ccaggaaggg tacctgtcac ctccagatta tcgagatcgg cttcggtata cagcggcttg
    19621 atggcgatcc cttccgcggt ttgctgaacc agcgagtcga cagttttctc ccgacggctc
    19681 aattccttgt tggcaagctg ttgccactcc tgctcgttag acattatttg ctccgtaaaa
    19741 cgcaatcaaa atgagggtat ttgcgcttac ggtgagcttt ttacgtgggt ttgtcgccag
    19801 caaaaaaaat gcattcagac gtcgtttttt gccgcctgag atttgtctat tttgtgatgt
    19861 gattcaagga atacaaaaag aagtgaggac aacgcctgat atgtgctatc aggcattatt
    19921 tgatggatta atcctgacga aggactttgt gaccataatc gagtaacgca tcagtgactt
    19981 tacgcatcat gcggctttca ggagcaaagc ggtgccagta gagcatccgt cgttgaaata
    20041 gcccaggcgt taagtcaatc agttcaccgc tggccagctc tttctcgatt tgcaggtgcg
    20101 ggatcataca gcaggtggtg ccctggcgag caagttgtac gaacgcttct gaagaattaa
    20161 cgatatggca gggtacgctg ccaggaggaa gatcgaagtt ttgctgcaaa aaggcctggt
    20221 gcatatcgtc aagatggtca aacgcgacca ctggcgcttt caataatgcc gaacgcgtta
    20281 cgccgttagg aaaatatttt tcggcaaacg gttttgagct gacgaacaga tagtcgagcg
    20341 caccgagttt atcgacaaga caactcggca gcgcctgatg ttgaatactc accgcgccga
    20401 ccacttcgcc gcggcgcaga cgttcctgag tacgggtttc atcttctacc tgcaagttga
    20461 ggcggatagg cgaatcagcc aacacaggag ccagtgccgg aagcaaccac gtcgccagac
    20521 tgtcggcgtt gaccgccagt gaaagcagca gcggagtcga accggtttgt tcatcgccca
    20581 gccactcttc ttccagcaac tccacctggc gcagcagtgc cagcagtttt tgcccttgtt
    20641 ccgtcgggcg cggcggtacg gtacgcacca acagcggctg cccgaacata ttttccagtt
    20701 gcttaatgcg ctgtgagacg gctgattgtg taatgcacag cttttgtgcc gcgcgctcaa
    20761 atcctcgttc acgtatcacc gcatccagtg cctgtaatgt tctgtagtcc gggcgtttca
    20821 ttgctgttgt ctgctcctgg taaatgcttt ttcctgcact atgacacaat tttgtgtcag
    20881 gttgcatata ccgaagccgt cggcgggtaa catttgtatc aatggttaaa cgcgcagctt
    20941 aaaagatcag ggatttgcgc tattgcattt cgcttagcga cttcgcgccc tggttatgtt
    21001 gaatggcgtg gcgttattgc ctctgttcgc accgtggagc ggcataccca gaccgttgta
    21061 tgctttattt gcggggcgaa aggggatgcc cgccattgcg cgatatttta tggatgagtt
    21121 aaccacccgg cttgccaacg gggtctgaat cgcttttttt gtatataatg cgtgtgaaat
    21181 ttcataccac aggcgaaacg atcatgacgc aggatgaatt gaaaaaagca gtaggatggg
    21241 cggcacttca gtatgttcag cccggtacca ttgttggtgt aggtacaggt tccaccgccg
    21301 cacactttat tgacgcgctc ggcacgatga aaggccagat tgaaggggcc gtttccagtt
    21361 cagatgcttc cactgaaaaa ctgaaaagcc tcggcattca cgtttttgat ctcaacgaag
    21421 tcgacagcct tggcatctac gttgatggcg cagatgaaat caacggccac atgcaaatga
    21481 tcaaaggcgg cggcgcggcg ctgacccgtg aaaaaatcat tgcttcggtt gcggaaaaat
    21541 ttatctgtat tgccgacgcc tccaagcagg ttgatattct gggtaaattc ccgctgccag
    21601 tagaagttat cccgatggca cgtagtgcag tggcgcgcca gctggtgaaa ctgggcggtc
    21661 gtccggaata ccgtcagggc gtggtgaccg ataatggcaa cgtgatcctc gacgtccacg
    21721 gcatggaaat ccttgacccg atagcgatgg aaaacgccat aaatgcgatt cctggcgtgg
    21781 tgactgttgg cttgtttgct aaccgtggcg cggacgttgc gctgattggc acacctgacg
    21841 gtgtcaaaac cattgtgaaa tgatctgacg ggggaacctc ccccgttaaa aaaattctct
    21901 tcattaaatt tggtgacatg tgtcacgctt ttaccaggca attgtcgatt gctctaaata
    21961 aatcctctaa accagcatat tcatccaaga attacctttg cgtgatattt cctcaacatc
    22021 gcgacgcaaa cgttcatatt gccgcaatat tattttttga tatgttgaaa ggcggatgca
    22081 aatccgcaca caacatttca aaagacagga ttgggtaaat ggcaaaggta tcgctggaga
    22141 aagacaagat taagtttctg ctggtagaag gcgtgcacca aaaggcgctg gaaagccttc
    22201 gtgcagctgg ttacaccaac atcgaatttc acaaaggcgc gctggatgat gaacaattaa
    22261 aagaatccat ccgcgatgcc cacttcatcg gcctgcgatc ccgtacccat ctgactgaag
    22321 acgtgatcaa cgccgcagaa aaactggtcg ctattggctg tttctgtatc ggaacaaacc
    22381 aggttgatct ggatgcggcg gcaaagcgcg ggatcccggt atttaacgca ccgttctcaa
    22441 atacgcgctc tgttgcggag ctggtgattg gcgaactgct gctgctattg cgcggcgtgc
    22501 cggaagccaa tgctaaagcg caccgtggcg tgtggaacaa actggcggcg ggttcttttg
    22561 aagcgcgcgg caaaaagctg ggtatcatcg gctacggtca tattggtacg caattgggca
    22621 ttctggctga atcgctggga atgtatgttt acttttatga tattgaaaac aaactgccgc
    22681 tgggcaacgc cactcaggta cagcatcttt ctgacctgct gaatatgagc gatgtggtga
    22741 gtctgcatgt accagagaat ccgtccacca aaaatatgat gggcgcgaaa gagatttcgc
    22801 taatgaagcc cggctcgctg ctgattaatg cctcgcgcgg tactgtggtg gatattccgg
    22861 cgctgtgtga tgcgctggcg agcaaacatc tggcgggggc ggcaatcgac gtattcccga
    22921 cggaaccggc gaccaatagc gatccattta cctctccact gtgtgaattc gacaacgtcc
    22981 ttctgacgcc acacattggc ggttcgactc aggaagcgca ggagaatatc ggcttggaag
    23041 ttgcgggtaa attgatcaag tattctgaca atggctcaac gctctctgcg gtgaacttcc
    23101 cggaagtctc gctgccactg catggtggtc gtcgtctgat gcacatccac gaaaaccgtc
    23161 cgggcgtgct aactgcgctg aacaaaatct tcgccgagca gggcgtcaac atcgccgcgc
    23221 aatatctaca aacttccgcc cagatgggtt atgtagttat tgatattgaa gctgacgaag
    23281 acgttgccga aaaagcgctg caggcaatga aagctattcc gggtaccatt cgcgcccgtc
    23341 tgctgtacta attccccttc tctgaaaatt aacgggcagg tcactgactt gcccgttttt
    23401 ttatcccttc tccacaccgt tgccgcgttg cgcattctac tttgcgagtc tgctccagaa
    23461 aataaatcaa agtcagatca atgcgttgtg tttatttaat ggggatttac gatggcggga
    23521 cagcatgggg ctgtaacggt aaagccctca ccgaagcgag ggcttaaagg agaagggtta
    23581 tgatgcgact tgtcatcata ctgattgtac tgttactgat aagtttcgga gcttattaac
    23641 agtcagtctc aagggaggag tcatcctccc ttacccttac tcactaaatt aggtcaaaga
    23701 atcaacgatg tcaatcaggg cgatgcgggg tgcatcgccc tgaaccgaac tttttgatag
    23761 ccacttcatc gcccacgttt actgccgtaa tagatccttt ttcccaaaaa ttatctctgt
    23821 tttgcatggg gtttttcgag cacgctcgaa gaaaagattt aaaggtagat caatgcgttg
    23881 tggttattta aagagagctt acgatagctg ggcagcatgg ggctgtaacg gtaaagccct
    23941 caccgaagcg agggcttgaa ggagaagggt tatgatgcga cttgccatca tactgattgt
    24001 actgttactc ataagtttcc cagcttatta acagtcagtc acaggggagg agcaatcctc
    24061 ccttaccctt actcactaaa ttaggttaaa gaatcaacga tgtcaatcag ggcgatgcag
    24121 gtgtatcgcc cttaccactc ccagactttc gacggtgtaa ccaccgcagg aagagggata
    24181 tcccactctt caacggggag tttttccacc aactgacaat catgcgcata acccaccggt
    24241 tgcgttttat agtgctgcca gttttgtaag gtccgatcat aaaaaccacc gcccattccc
    24301 agacgctgac cgtactcatc aaaggcgacc agcggtgtga tcagcacgtc taatcgggaa
    24361 aggggtagca cgtcacgcac atccagtttt ggctcatgga tcttcaacct gttcatcacc
    24421 agttcgcttt gcggatggta attcaggaac agcaaattac cggcactaaa gggatgcaaa
    24481 actggaagat atacgcgctt accggcgcgc cagagttgtt ctattagcgg ctgggtgtcg
    24541 agttcaccat caaaagagag gaatacagcg accgtatgcg ccatcaccac cgggggataa
    24601 gtcatcatcc gggtagcggc ttgttgaccc atttccagct gttgttccgg cgttaacgca
    24661 cgacgacgtt gccgaatcat tttgcggatt tcttgtcggg ataatgtcag tgggagctct
    24721 ggtagttgcg tcatggctgg tgccagataa gaagggaatc tccgagatgc cgccgcaggc
    24781 tgtaaccctt gaacccttgg ttcaaggtga atgtgtcgtc gcagttttaa ggcttctcgg
    24841 acgaaccgag catgctcacc aaccgcggag cgccacattc ttgtggtatg aaatatcggc
    24901 tcaggggact ggcccgcttg cgaacatctc agagaaattt tgtcttcacg gttactctac
    24961 cacagtaaac cgaaaagtgt cattcaaagt tttggttagt tttttcggtg atgcgacctt
    25021 gttcaagtaa cgcttgttct atggtctgct gcagcatccg aatacgctgt tccatacttg
    25081 ccgcgtagtc acgagtcttt gctttttctt gcgctaactc atagctgata ttcaatgcgg
    25141 caatgaagac caactgttca gtatttgtga ctctagtgcg ttctttcaga tcttgcaacc
    25201 gttggttcag atcgtccgct gcctgattca acgcatccct ttggtcaggc gggcagttca
    25261 cacgcagtga acggccaaaa atttggatat cgacgggttg tgcagacatg ccaccttcct
    25321 gctgattgac tgcgctgcct tcgtctttag acccatggtc tgcgaagggg cgacactata
    25381 gctaccctga tgagaagaga caagcccttt tctggtccac cagggcccaa agtggtagca
    25441 tatcatgaat attcctccct ttgacgacga atgcttatgt ctatacagaa cgaaatgcct
    25501 ggttacaacg aaatgaacca gtatctgaac caacaaggga cgggtctgac ccctgccgag
    25561 atgcatggtt taatcagtgg gatgatatgt ggcggtaacg atgacagctc atggctgccg
    25621 ctacttcacg acctgacgaa cgaaggcatg gctttcggtc atgagctggc acaggcactg
    25681 cgtaaaatgc actctgccac cagcgatgcc ctgcaggatg acggcttcct ttttcagctt
    25741 tatctgcctg atggcgatga tgtcagcgtt ttcgatcggg ctgatgcact ggcaggttgg
    25801 gtcaatcact tcctgcttgg tcttggcgtt acgcaaccga agctggacaa agtgaccggc
    25861 gaaaccggtg aagccatcga cgatctgcgt aacatcgcgc agctgggcta cgacgaagac
    25921 gaagatcagg aagagcttga aatgtcgctt gaagagatca tcgaatacgt tcgtgttgcc
    25981 gcgctgttat gccacgacac ctttactcat ccgcaaccga ccgcgccaga agtacaaaaa
    26041 ccgactctac actaaaaaca aaaaacgtaa ggagagtgtt atgagtgaga tatcccggca
    26101 agagtttcag cgtcgccgtc aggccctggt ggagcaaatg caacccggca gcgccgcgct
    26161 gatttttgct gcaccagaag taacacgtag cgccgacagc gaatacccct atcgtcagaa
    26221 cagtgacttc tggtacttca ccggctttaa cgaaccggaa gcggtgctgg tgctgattaa
    26281 aagcgatgac actcataacc acagcgttct gtttaaccgc gttcgcgacc tgacggcgga
    26341 gatctggttt ggccgtcgct taggccagga tgccgcgcca gagaaactgg gcgttgaccg
    26401 cgcactggca ttcagcgaaa tcaatcagca actttatcaa ctacttaacg gcctggatgt
    26461 ggtttaccat gcccagggcg aatatgcata tgctgatgaa atcgtgaaca gtgcgctgga
    26521 aaaactgcgt aaaggttcgc ggcaaaatct caccgcaccg gcaacgatga tcgactggcg
    26581 tcctgttgtt catgaaatgc gcctgttcaa atcgccagaa gagattgccg tactccgccg
    26641 cgcgggagaa atcaccgcca tggcacatac acgggcgatg gaaaaatgcc gtccgggaat
    26701 gttcgagtac catctggaag gcgaaattca ccacgaattt aaccgccacg gtgcgcgcta
    26761 tccttcctat aacaccattg taggcagcgg tgaaaacggc tgcattctgc actacaccga
    26821 aaacgagagt gaactgcgcg acggtgacct ggtgttgatt gacgcgggtt gtgaatacaa
    26881 aggttacgcg ggcgatatta cccgcacctt cccggtcaac ggcaaattca cccaggccca
    26941 gcgtgaaatc tacgacattg tgctggagtc tctcgaaacc agcctgcgcc tgtatcgtcc
    27001 gggaacttcc attctggaag tcactggtga agtggtgcgc atcatggtta gcggcctggt
    27061 aaaactcggc atcctgaaag gtaatgttga tgaactgatc gctcagaacg cccatcgtcc
    27121 tttctttatg catggcctta gccactggtt aggactggat gtccatgacg ttggcgttta
    27181 tggtcaggat cgctcgcgca ttctggaacc ggggatggta ctgaccgtag agcctggtct
    27241 gtatatcgcg ccggatgcgg atgtgccaga acaatatcgc ggtatcggca ttcgtattga
    27301 agacgacatt gtgattaccg aaaccggcaa cgaaaacctc accgccagcg tggtgaaaaa
    27361 gccggaagaa atcgaagcgt tgatggctgc tgcgagaaag caatgagcgt aatcatcgtc
    27421 ggtggcggca tggcgggcgc gacgctggcg ctggctattt cccggttaag tcacggggcg
    27481 ctgccggtac atttgattga agcgactgcg ccagagtcac atgctcatcc gggctttgat
    27541 ggacgagcga tagcgctggc ggcgggtacc tgtcagcaac tggcgcgcat cggcgtctgg
    27601 caatctctgg ctgattgcgc aaccgccatc actaccgtgc atgtcagcga tcgtggtcat
    27661 gctggattcg tcaccctcgc cgcagaagat taccaactgg cggcgctggg gcaggttgtc
    27721 gaattgcata atgtcgggca acggctgttt gcattgctgc gaaaagcacc tggcgtaatg
    27781 ctgcattgcc ccgatcgcgt agctaacgtt gcccgtactc agagtcatgt tgaagtgacg
    27841 ctggagagcg gcgagatgct gacgggccgc gtgctgatag ccgctgatgg cacccattca
    27901 gcgttagcca ccgcgtgcgg cgttgattgg cagcaggagc cttacgaaca actggcagtg
    27961 attgccaacg ttgctacttc tgttgcgcac gaaggccgcg cttttgaacg cttcacgcaa
    28021 catggcccgc tggcgatgtt gccgatgtct gacggacgct gttcgctggt ctggtgtcat
    28081 ccactggaac ggcgcgaaga ggtgctgtcg tggagcgacg agaagttttg ccgtgaactc
    28141 cagtctgcct ttggctggcg actggggcaa attacccacg ccggtaaacg cagtgcttat
    28201 ccgctggcat taacccgcgc cgccagaccg attacccatc gcactgtgct ggtgggcaat
    28261 gcggcgcaaa ctctgcaccc gattgccggg caagggttta acctcggtat gcgagatgtc
    28321 atgagccttg cggaaaccct gactcaggcg catgagcgcg gagaagacat cggtgattac
    28381 ggcatattgt gccgctatca gcagcgtcga cagagcgatc gcgaagcaac catcggcgtc
    28441 acggacagcc tggtacatct ttttgccaac cgttgggcac cgctggttgt cgggcgcaac
    28501 atcggactga tgacgatgga attattcacc ccggcacgcg atgtgctggc gcagcgcacc
    28561 ctcggttggg tggcgcgttg aaggcggttt taaggaataa acaatgcaaa gtgttgatgt
    28621 agccattgtt ggcggcggca tggtggggct ggcggttgcc tgcggcttac aggggagcgg
    28681 cttacgcgtt gccgtactgg agcagcgcgt accggaacct ctggcggtag atgcactacc
    28741 acaactgcgc gtttcggcta tcaatgccgc cagcgaaaaa ttactcaccc gtcttggcgt
    28801 ctggcaggac attctcgctc gtagggccag ttgttatcac ggcatggaag tgtgggacaa
    28861 agacagtttt ggtcacatct cgtttgacga tcaaagcatg ggctatagcc atcttgggca
    28921 catcgttgaa aactcagtga ttcactacgc gctgtggaac aaagcgcagc agtcgtcaga
    28981 tattactctg ttggccccca cagaattaca gcaggtcgcc tggggagaaa atgaaacctt
    29041 cctgacgctg aaagatggca gcatgttaac ggcgcgtctg gtgattggcg cggacggtgc
    29101 taattcctgg ttgcgcaaca aagcagatat tccgctgact ttctgggatt atcagcatca
    29161 cgcgctggta gcgaccattc gcacggaaga accgcatgat gcggtggcgc ggcaggtttt
    29221 ccatggcgaa ggcattctgg cctttttacc gcttagcgat ccgcatcttt gctcgattgt
    29281 ctggtcactg tcgccagagg aagcgcagcg gatgcagcag gcaagtgaag acgaatttaa
    29341 tcgcgcgtta aatatcgctt ttgataatcg ccttggctta tgcaaggttg agagcgcgcg
    29401 tcaggtgttc ccgctgacgg ggcgttatgc gcgccagttt gccgcacacc gtctggcgct
    29461 ggtgggcgac gccgcgcata ccattcaccc actggcgggg cagggcgtta atctcggatt
    29521 tatggacgcc gcagagctga ttgctgaact gaaacggttg catcgtcagg gtaaagacat
    29581 cgggcagtac atttacctgc gccgctatga acgtagccgc aagcacagcg cggcgctgat
    29641 gctggctggt atgcagggtt ttcgcgatct cttttccggc gctaatccgg cgaaaaaact
    29701 gctgcgtgat attggtttga aactggccga cacgcttcct ggcgttaagc cgcaacttat
    29761 ccgccaggca atgggattaa acgatttgcc tgaatggctg cgttaaaaat ttctcctctg
    29821 ttgtttattt gatacccatc acactttcat ctcccggttt tttcgccggg agattttcct
    29881 catttgaaat aaactaattt cacctccgtt ttcgcattat attttctaat accatgattt
    29941 tttgatttag tgttttttga cattttttta gctcttaata ttgtcttatt caaattgact
    30001 ttctcatcac atcatctttg tatagaaatt ggtgtatttt ttggtttttt attctgtcgc
    30061 gatttttgca ttttttaacc ataagctaat gtgatgatca attttacctt atggttaaca
    30121 gtctgtttcg gtggtaagtt caggcaaaag agaacgattg cgttggggac cgggagtggc
    30181 tccgatgctg ggttttgtgg tgataatttc accatgaaaa agttgtcagc cccgcttatt
    30241 caatgaggac aagatggcac aacagactcc tttgtacgaa caacacacgc tttgcggcgc
    30301 tcgcatggtg gatttccacg gctggatgat gccgctgcat tacggttcgc aaatcgacga
    30361 acatcatgcg gtacgtaccg atgccggaat gtttgatgtg tcgcatatga ccatcgtcga
    30421 tctccgcggc agccgcaccc gggagtttct gcgttatctg ctggcgaacg atgtggcgaa
    30481 gctcaccaaa agcggcaaag ccctttactc ggggatgttg aatgcctctg gcggtgtgat
    30541 agatgatctc atcgtctact actttactga agatttcttc cgcctcgttg ttaactccgc
    30601 cacccgcgaa aaagacctct cctggattac ccaacacgct gaacctttcg gcatcgaaat
    30661 taccgttcgt gatgaccttt ccatgattgc cgtgcaaggg ccgaatgctc aggcaaaagc
    30721 tgccacactg tttaatgatg ctcagcgtca ggcggtggaa gggatgaaac cgttctttgg
    30781 cgtgcaggtg ggcgatctgt ttattgccac cactggttat accggtgaag cgggctatga
    30841 aattgcgctg cccaatgaaa aagcggccga tttctggcgt gcgctggtgg aagcgggcgt
    30901 taagccctgc ggtctggggg cgcgtgacac gctgcgtctg gaagcgggca tgaatcttta
    30961 tggtcaggag atggacgaaa ctatttctcc tttagccgcc aacatgggct ggactatcgc
    31021 ctgggaaccg acagatcgtg actttatcgg tcgtgaagcc ctggaagtgc agcgtgagca
    31081 tggtacagaa aaactggttg gtctggtgat gaccgaaaaa ggcgtgctgc gtaatgaact
    31141 gccggtacgc tttaccgatg cgcagggcaa ccagcatgaa ggcattatca ccagcggtac
    31201 tttctccccg acgctgggtt acagcattgc gctggcgcgc gtgccggaag gtattggcga
    31261 aacggcgatt gtgcaaattc gcaaccgtga aatgccggtt aaagtgacaa aacctgtttt
    31321 tgtgcgtaac ggcaaagccg tcgcgtgatt tacttttttg gagattgatt gatgagcaac
    31381 gtaccagcag aactgaaata cagcaaagaa cacgaatggc tgcgtaaaga agccgacggc
    31441 acttacaccg ttggtattac cgaacatgct caggagctgt taggcgatat ggtgtttgtt
    31501 gacctgccgg aagtgggcgc aacggttagc gcgggcgatg actgcgcggt tgccgaatcg
    31561 gtaaaagcgg cgtcagacat ttatgcgcca gtaagcggtg aaatcgtggc ggtaaacgac
    31621 gcactgagcg attccccgga actggtgaac agcgaaccgt atgcaggcgg ctggatcttt
    31681 aaaatcaaag ccagcgatga aagcgaactg gaatcactgc tggatgcgac cgcatacgaa
    31741 gcattgttag aagatgagta acggctttat tcctcttctg cgggagagga tcagggtgag
    31801 gaaaatttat gcctcaccct cactctcttc gtaaggagag aggttcacaa ttcactgcac
    31861 gtttcaggaa ccatcgctca tgacacagac gttaagccag cttgaaaaca gcggcgcttt
    31921 tattgaacgc catatcggac cggacgccgc gcaacagcaa gaaatgctga atgccgttgg
    31981 tgcacaatcg ttaaacgcgc tgaccggcca gattgtgccg aaagatattc agcttgcgac
    32041 accaccgcag gttggcgcac cggcgaccga atacgccgca ctggcagaac tcaaggctat
    32101 tgccagtcgc aataaacgct tcacgtctta catcggcatg ggttacaccg ccgtgcagct
    32161 accgccggtt atcctgcgta acatgctgga aaatccgggc tggtataccg cgtacactcc
    32221 gtatcagcct gaagtctccc agggccgcct tgaagcactg ctcaacttcc agcaggtaac
    32281 gctggatttg actggactgg atatggcctc tgcttctctt ctggacgagg ccaccgctgc
    32341 cgccgaagca atggcgatgg cgaaacgcgt cagcaaactg aaaaatgcca accgcttctt
    32401 cgtggcttcc aatgtgcatc cgcaaacgct ggatgtggtc cgtactcgtg ctgaaacctt
    32461 tggttttgaa gtgattgtcg atgacgcgca aaaagtgctc gaccatcagg acgtcttcgg
    32521 cgtgctgtta cagcaggtag gcactaccgg tgaaattcac gactacactg cgcttattag
    32581 cgaactgaaa tcacgcaaaa ttgtggtcag cgttgccgcc gatattatgg cgctggtgct
    32641 gttaactgcg ccgggtaaac agggcgcgga tattgttttt ggttcggcgc aacgcttcgg
    32701 cgtgccgatg ggctacggtg gcccacacgc ggcattcttt gcggcgaaag atgaatacaa
    32761 acgctcaatg ccgggccgta ttatcggtgt atcgaaagat gcagctggca ataccgcgct
    32821 gcgcatggcg atgcagactc gcgagcaaca tattcgtcgt gagaaagcga actccaacat
    32881 ttgtacttcc caggtactgc tggcaaacat cgccagcctg tatgccgttt atcacggccc
    32941 ggttggcctg aaacgtatcg ctaaccgcat tcaccgtctg accgatattc tggcggcggg
    33001 cctgcaacaa aaaggtctga aactgcgcca tgcgcactat ttcgacacct tgtgtgtgga
    33061 agtggccgac aaagcgggcg tactgacgcg tgccgaagcg gctgaaatca acctgcgtag
    33121 cgatatcctg aacgccgtgg ggatcaccct tgatgaaacc accacgcgtg aaaacgtaat
    33181 gcagcttttc agtgtgctgc tgggcgataa ccacgggctg gagatcgaca cgctggacaa
    33241 agacgtggct cacgacagcc gctctatcca gcctgcgatg ctgcgcgacg acgaaatcct
    33301 cacccatccg gtgtttaatc gctatcacag cgaaaccgaa atgatgcgct atatgcactc
    33361 gctggagcgt aaagatctgg cgctgaatca ggcgatgatc ccgctgggtt cctgcaccat
    33421 gaaactgaac gccgccgccg agatgatccc aatcacttgg acggaatttg ccgaactgca
    33481 cccgttttgc ccgccggaac aggctgaagg ttatcagcag atgattgcgc agctggctga
    33541 ctggctggtg aaactgaccg gttacgacgc cgtttgtatg cagccgaact ccggcgcaca
    33601 gggcgaatac gcgggcctgc tggcgattcg tcattatcat gaaagccgca acgaagggca
    33661 tcgcgatatc tgcctgatcc cggcttctgc gcacggaact aaccccgctt ctgcacatat
    33721 ggcaggaatg caggtggtgg ttgtggcgtg tgataaaaac ggcaacatcg atctgactga
    33781 tctgcgcgcg aaagcggaac aggcgggcga taacctctcc tgtatcatgg tgacttatcc
    33841 ttctacccac ggcgtgtatg aagaaacgat ccgtgaagtg tgtgaagtcg tgcatcagtt
    33901 cggcggtcag gtttaccttg atggcgcgaa catgaacgcc caggttggca tcacctcgcc
    33961 gggctttatt ggcgcggacg tttcgcacct caacctgcat aaaactttct gcattccgca
    34021 cggcggtggt ggtccgggta tgggaccgat cggtgtgaaa gcgcatctgg caccgtttgt
    34081 accgggtcat agcgtggtgc aaatcgaagg catgttaacc cgtcagggcg cagtttctgc
    34141 ggcaccgttc ggtagcgcct ctatcctgcc aatcagctgg atgtacatcc gcatgatggg
    34201 cgcagaaggg ctgaaaaaag caagccaggt ggcaatcctc aacgccaact atattgccag
    34261 ccgcctgcag gatgccttcc cggtgctgta taccggtcgc gacggtcgcg tggcgcacga
    34321 atgtattctc gatattcgcc cgctgaaaga agaaaccggc atcagcgagc tggacattgc
    34381 caagcgcctg atcgactacg gtttccacgc gccgacgatg tcgttcccgg tggcgggtac
    34441 gctgatggtt gaaccgactg aatctgaaag caaagtggaa ctggatcgct ttatcgacgc
    34501 gatgctggct atccgcgcag aaattgacca ggtgaaagcc ggtgtctggc cgctggaaga
    34561 taacccgctg gtgaacgcgc cgcacattca gagcgaactg gtcgccgagt gggcgcatcc
    34621 gtacagccgt gaagttgcgg tattcccggc aggcgtggca gacaaatact ggccgacggt
    34681 gaaacgtctg gatgatgttt acggcgaccg taacctgttc tgctcctgcg taccgattag
    34741 cgaataccag taattcactg attcgagtat tttctaaagg cgcttcggcg cctttttagt
    34801 cagatgacaa agtacaaaag tgctcagaca gtcccctcgc ccctttgggg agagggttag
    34861 ggtgagggga acaggccggc actggcgcga atatttaccc tcaccccgac cctctccctg
    34921 aaagggcgag ggggaaaagc gtgccaacat tgaagattga gccagtttgt tagcaatctc
    34981 aaagatacgt caacgaatta atttttctcg gaaaaacaaa tggctatagc acttgtgacc
    35041 ggtggcagtc gcggcatcgg gcgggcaact gcattactgt tggcgcaaga agggtatacg
    35101 gtggcggtta attatcagca aaacctccac gcggcgcagg aagtgatgaa cttaataatg
    35161 caagccggtg gcaaagcatt cgtgctccag gcggatatca gcgacgaaaa ccaggtcgtt
    35221 gcgatgttta cagcaatcga tcagcacgat gaaccgctag cagcgctggt caataacgcc
    35281 gggatcttgt ttacccagtg caccgttgaa aaccttaccg cagagcgaat caaccgagta
    35341 ctttccacca acgtgacggg atattttctc tgctgccgcg aggcggtaaa acgcatggcg
    35401 cttaaaaatg gtggcagtgg cggcgctatc gtcaatgtct cttcggtggc ctcacggttg
    35461 ggttcgccag gggaatatgt tgattacgcg gcatcgaaag gggcgattga ttcgttaacc
    35521 accggactat cgctggaagt cgccgcgcag gggatccgcg ttaactgcgt gcggccaggg
    35581 tttatttata ccgaaatgca cgccagcggc ggcgagcctg gacgcgtcga tcgcgttaag
    35641 tcgaacatcc ccatgcagcg tggtggacag gcagaagagg tcgcgcaggc cattgtctgg
    35701 ctactaagtg ataaagcctc ttacgtcacg ggaagtttta tcgatttggc gggcgggaaa
    35761 taaaacaggg aagttgtctg accggatgca acaagtattg catccggtac ttcatcgact
    35821 taaagcttct cgccgttgct ggcaatcacc tctttgtacc agttaaagct cttcttacgt
    35881 gaacgcgaca tatcgccagt accgtcgtca tgtttattca catagataaa gccgtagcgt
    35941 ttgctgtact gcccggtagt gaatgacacg cagtcgatgc agccccacgg tgtgtagccc
    36001 atcagatcca caccatcgta agtcactgct tttttcatct cttcaatatg ggcgcgcagg
    36061 tagtcaatgc ggtagtcgtc gttgatgctg ccatcttctt ccactttgtc gtaagcgcca
    36121 aaaccgtttt cgacaataaa cagcggcctc tgataacgct catacagttc gcaaagggca
    36181 tagcgcagac ctactggatc aatctgccag ccccagtcag acgctttaac atacgggttc
    36241 ggtacgctgc cttcaaaacc agagatcgca tcgccggtgc cgccttcggc cttcactgcg
    36301 ttggtcatgt aatagctgaa accaagataa tcgcaggtgc cttcgcgcag tacatcgaga
    36361 tcgccgtctt ccattttgat attaaatccg cgacgttccc actcgttcaa tacataggac
    36421 gggtaatagc cgcatagctg cacatcggta aagacgtagc gttcgcgcat cgactcctga
    36481 gcgaacatca catcgtccgg gttacatgag tagggataga gcggcaccat cgccagcata
    36541 cagccgactt tcatctccgg gttaatgcga cgcgcggctt tcactgccag ggcgctggcg
    36601 acaaactggt gatgcagcac ctggtacatc gtctcttccg ggttgtcatg ctcggtatac
    36661 accacgccgg agcagcagta accaaacagc ggcgcacgcc agttacgctg gttgtttatt
    36721 tcgttgaagg tcatccagta tttgacttta tgcttgtagc gttcgaatac cacttccgcg
    36781 aaacggacaa agaaatcaac gaatttacgg ttggtccagc taccgtattg ctgcaccaga
    36841 tgcagcggca tttcaaagtg ggagagggtt atcaccggtt cgatgttgta tttcagcagt
    36901 tcatcgaaca gagagtcgta aaacttcagc ccttcttcgt ttggctgagc ttcatcacct
    36961 tttggaaaaa tacgcgtcca ggcgatggaa gtccgaaaac atttgaagcc catttcggca
    37021 aatagcttga tgtcttcctt gtagtgacca taaaaatcaa cggcttcatg gtttggatag
    37081 tattttcctg gcaagacttc tttggtgatt tcgcgcggca cgccgtgtgc gccacaggtc
    37141 agaacgtcac aaatgctcgg tcctttgccg cctttgttcc agccgccttc gacctgatga
    37201 gcggcaacgg cgccacccca taagaaatct ttcggtaagg tgagtttttt cactatcata
    37261 ggctccagat cagtcaattc ttacaggaaa tagtaccatg cagccaaacg acatcacttt
    37321 tttcaacgtt tccaggatga cattctggct gggcgtaaaa ccatcaccat ccgcgacgag
    37381 tctgaatcgc acttcaaaac gggtgatgtg cttcgtgtcg gacgttttga agatgacggt
    37441 tatttttgca cgattgaagt caccgcaacc tcaaccgtaa cgctggatac actgacggaa
    37501 aaacatgcag agcaggaaaa tatgaccctg cctgaactga aaaaggtcat tgctgacatc
    37561 tatcccggtc agacacaatt ttatatgatt gaatttaaat gtctttaaat tgaactcacg
    37621 cctgttagtt gaaatttcgc atttaatttc atgattttta ggagattagt ctcaaatcag
    37681 aatttatttt agctaacagg tgttcactgg aactattctc agttacgctg gagaggtata
    37741 catgtgcgtg tacgtttctc tggagtaagt tatggttcag aagcccctca ttaagcaggg
    37801 atattcgctg gcagaggaaa ttgccaacag cgtcagtcac ggcattgggc tggtgtttgg
    37861 tatcgttggg ctggtgttgc tactggttca ggcggtggat ctcaatgcca gcgccacagc
    37921 gataaccagt tacagcctct atggcggcag tatgatcctg ctgttcctcg cttcgacgct
    37981 ctatcacgcc attcctcatc aacgggcaaa aatgtggctg aagaaatttg accattgcgc
    38041 tatttacctg ttgattgccg gaacctacac gccgtttttg ctggtggggc tggattctcc
    38101 gttagcgcgc gggttgatga ttgttatctg gagcctagca ttgctgggga ttctgttcaa
    38161 actgaccatc gcgcaccgat tcaaaatttt atctctggtg acttatctgg cgatgggctg
    38221 gctgtcgctg gtggtaattt atgaaatggc agttaagctc gcggcgggca gcgttacctt
    38281 actggcggta ggcggcgtgg tttattcgct cggggtgatt ttctacgtct gcaaacgcat
    38341 tccatacaac catgccatct ggcacggctt cgtgctcggc ggtagtgtgt gccactttct
    38401 ggcgatctat ttgtatattg ggcaggcgta atgggtatgc ctttgtaggc ctgataagac
    38461 gcgtcagcgt cgcatcaggc atcggagcgc aaccgccgga tgcggcgtga acgccttatc
    38521 cgatctacgg gcctcgtccc tgttgtgata aaggcatcaa atcccagaca tgataagacg
    38581 cgtcagcgtc gcatcaggcg ataaatgcct gatgcgatac agagatttac tcttcgagcg
    38641 aatacggcag cggctcgata tgcagtgtat tcgcatcgtc gcgtacgcgg aagatgctgt
    38701 ccggttccat atcgttattc atgacgacct gtaccacgac ctgaccatct tccagtttta
    38761 ccgcagccag caccgtaccg gtacgacgcc agttctcgcc cattttcagc tctaagtctt
    38821 caccagcttc cggcagtcgg ctggcactac ctgccagcaa ccagagcgcg cgtttgttgg
    38881 caccacggaa ttttgctcgc gccaccatct cttgtccggt ataacagcct ttcttaaagc
    38941 tgataccgcc cagcgcctgg agattggtcg cctgtgggat aaactgcccg ctgttggcgg
    39001 catcaatcac cgggaaacca gcttcaatgt ttaatgccag ccactgttgg ctattgttca
    39061 gttccgcttc accgcgcagt ttatcggtca gcatattagc agtagcttca tcggttacga
    39121 tcaggaaacg ttctgccggg tgttcaaacc atagcaaagt ggtcgcgcct tctttgacta
    39181 cctgtttttc tttcgaaggc agttcgctaa agagatttgc cagcgcggcg cgcgcctgaa
    39241 aaccggcaac accaagcagc acacgctcgt cgtctggcgc gatggtcact ttagagaata
    39301 ccgcatattt tttcagttca gtcagctgcg gttcacgcac gctgcgccgt tcaatccatg
    39361 caaagccatc gccgtcgcgg aacagacgta aattgctcca cattttacct ttggcgtcgc
    39421 aatgggcggc gagcaggtgc tgatcttctg ccatctggct gacatctgct gtcacctgac
    39481 cctgcatata tttttcgctg tccgcgccag taatggtggc aagcgcccag tcatcaagcg
    39541 tcatcagcgt cagcggtaaa cgggcagaag ccgtaggctg acgtggagga aaaggtgtaa
    39601 aagccataat agagtcctga gttgcttaac ggcgtattaa tgactaatgg taaaagagct
    39661 atgaggcaat gcaagtgctt tcactacgcg atctgtgcct tctgcgaggc gactgccaaa
    39721 aaaatgaagc ccttgaattg ttgtgaatta atttcgcgat cgcgatggtg tttactgata
    39781 ttcccgtaaa agaagtcgaa aaacacgtta caatagccgg gtactggatt ttcgtgagac
    39841 aggaagaaga tggacattaa caacaaagcc cgaattcatt gggcatgccg ccgtggtatg
    39901 cgcgaactcg atatttcaat catgccgttt ttcgaacatg agtacgacag cttaagcgat
    39961 gacgaaaaac gcatctttat tcgtctgctg gaatgtgacg atccggacct gtttaactgg
    40021 ctgatgaatc acggtaaacc ggccgatgca gaactggaaa taatggtccg actcatccag
    40081 acacggaacc gggaacgtgg tcctgtggca atctgatttg cgcgtctcct ggcgcgcaca
    40141 gtggctttcc ttgctgattc atgggctggt tgccgctgtt attttactca tgccctggcc
    40201 gctcagttac accccgttat ggatggtgtt actttcgctg gtggtatttg attgcgttcg
    40261 cagccagcgg tgtattaatg ctcgccaggg ggaaattcgc ttgttgatgg acgggcgttt
    40321 gcgttggcaa gggcaggagt ggagcatcgt caaagcaccg tggatgatta agagcggcat
    40381 gatgctgcgt ttacgttctg atggcggtaa acggcaacat ttatggctgg cagccgacag
    40441 catggacgaa gccgaatggc gggatttacg gcggattttg ttgcaacaag agacgcaaag
    40501 ataaagatga cggcaaagca tatgcgctaa gccgtcagtg agatcaggcg taatgctctg
    40561 ccatttcgtt gaggatttgc tcgcaccagc tctgaatacg ctcgtcgcta aggtcatact
    40621 ggttagtttc atccagcgcc agacccacga acagttgccc gtcagcaatc accggtttcg
    40681 ggctggtaaa ttcatatcct tccgttggcc agtagccgac gaacttcacg cctttggtcg
    40741 agagtttgtc atgcagcata ccgagcgcat cgaggaacca ctcgccgtat cccagttgat
    40801 cgccaagccc atacagcgca ataattttac cttcaaggtt caggtcgtcg agctgatccc
    40861 agacggcttc ccagtcttcc tggatttcac caaaatccca ggtcgggata cccagaatga
    40921 gcacatcgta ctgctccatt aatttcgggg agtcgtcctt gaggttatgt aaggtcacca
    40981 gttctgggcc gataatatcg cggatttttt ctgccgccat ttcggtgtaa caggtgctgg
    41041 aaccgtaaaa aagacccata ttcataacgt aagcttctcg attctgctgt ggcttttgca
    41101 gctagtgtat cagaatcgct ttagtcacgg cacaataagg cataatggat caaatgagtg
    41161 accataaggg gcgcaagtga aacaggagct ggcacgcatc gagcagtttc ttgatgctct
    41221 gtggctggaa aaaaatctgg ctgaaaatac gttgaacgct taccgtcgcg atctgtcaat
    41281 gatggtggag tggttgcatc accgcgggtt gacgctggcg acggcgcaaa gtgacgattt
    41341 gcaggcatta ctggcagaac ggctggaggg cgggtataaa gccaccagct cagcgcgttt
    41401 gctgagtgca gtgcgccgat tgttccagta tctttatcgc gaaaagtttc gtgaagacga
    41461 tcccagtgcg catctcgctt caccgaaatt gccccagcgt ttgccaaaag atttaagcga
    41521 agcgcaggtc gaacgtttat tacaggcgcc attaattgat cagccactgg agctacgcga
    41581 taaagccatg cttgaagtgt tgtatgctac cgggctgcgt gtctctgaac tggtcgggct
    41641 gacaatgagt gatatcagcc tgcgtcaggg cgtagtgcgg gtcattggta aaggcaacaa
    41701 agagcgcctg gtgccgttag gggaagaggc ggtttactgg ctggaaacct atctggaaca
    41761 tgggcgtccg tggctgctga atggtgtgtc aattgacgtg ttgtttccca gccagcgtgc
    41821 gcagcagatg acgcgacaga ccttctggca ccgcattaaa cattatgccg tgctggcggg
    41881 tatcgacagc gaaaagctgt caccgcatgt gttgcgtcac gcttttgcca ctcatttatt
    41941 aaatcatggt gcggatttac gcgtggtgca gatgctactg ggccacagcg atctttccac
    42001 cacgcaaatt tatacgcatg tcgctaccga gcgtctgcgg caacttcatc aacagcatca
    42061 cccgcgggcg tgatgtctga aaagaacggg aagatttatg aagaaaggtt ttatgttgtt
    42121 tactttgtta gcggcgtttt caggctttgc tcaggctgat gacgcggcaa ttcaacaaac
    42181 gttagccaaa atgggcatca aaagcagcga tattcagccc gcgcccgtag ctggcatgaa
    42241 gacagttctg actaacagcg gtgtgttgta catcaccgat gatggtaaac atatcattca
    42301 ggggccaatg tatgacgtta gtggcacggc tccggtcaat gtcaccaata agatgctgtt
    42361 aaagcagttg aatgcgcttg aaaaagagat gatcgtttat aaagcgccgc aggaaaaaca
    42421 cgtcatcacc gtgtttactg atattacctg tggttactgc cacaaactgc atgagcaaat
    42481 ggcagactat aacgcgctgg ggatcaccgt gcgttatctt gctttcccgc gccaggggct
    42541 ggacagcgat gcagagaaag aaatgaaagc tatctggtgt gcgaaagata aaaacaaagc
    42601 gtttgatgat gtgatggcag gtaaaagcgt cgcaccagcc agctgcgacg tggatattgc
    42661 cgaccattac gcacttggcg tccagcttgg cgttagcggt actccggcag ttgtgctgag
    42721 caatggcaca cttgttccgg gttaccagcc gccgaaagac atgaaagaat tcctcgacga
    42781 acaccaaaaa atgaccagcg gtaaataatt cgcgtgaaac aacagataca acttcgtcgc
    42841 cgtgaagtcg atgaaacggc agacttgccc gctgaattgc ctcccttgct gcgccgttta
    42901 tacgccagcc ggggagtacg cagtgcgcaa gaactggaac gcagtgttaa aggtatgttg
    42961 ccctggcagc aactaagcgg tgtcgaaaag gccgttgaga tcctttacaa cgcttttcgc
    43021 gaaggaacgc ggattattgt ggtcggtgat ttcgacgccg acggcgcgac cagcacggct
    43081 ctaagcgtgc tggcgatgcg ctcgcttggt tgcagcaata tcgactacct ggtaccaaac
    43141 cgtttcgaag acggttacgg cttaagcccg gaagtggtcg atcaggccca tgcccgtggc
    43201 gcgcagttaa ttgtcacggt ggataacggt atttcctccc atgcgggagt tgagcacgct
    43261 cgctcgttgg gcatcccggt tattgttacc gatcaccatt tgccgggcga cacattaccc
    43321 gcagcggaag cgatcattaa ccctaacttg cgcgactgta atttcccgtc gaaatcactg
    43381 gcaggcgtgg gtgtggcgtt ttatctgatg ctggcgctgc gcaccttttt gcgcgatcag
    43441 ggctggtttg atgagcgcgg catcgcaatt cctaacctgg cagaactgct ggatctggta
    43501 gcactgggga cagtagcgga cgtcgtgccg ctggacgcta ataatcgcat tctgacctgg
    43561 caggggatga gtcgcatccg tgccggaaag tgccgtccag ggattaaagc gctgctggaa
    43621 gtggcaaatc gcgatgcaca aaaactagcc gccagcgatt taggttttgc gctgggaccg
    43681 cgtctcaatg ctgccgggcg actggatgat atgtccgttg gtgtggcgct gttgctgtgc
    43741 gacaacatcg gcgaagcgcg cgtgctggca aatgaactcg atgcgctaaa ccagacgcga
    43801 aaagagatcg aacaaggaat gcaggttgaa gccctgaccc tgtgcgagaa actggagcgc
    43861 agccgtgaca cgctacccgg cgggctggca atgtatcacc ccgaatggca tcagggcgtt
    43921 gtcggtattc tggcttcgcg tatcaaagag cgttttcacc gtccggttat cgcctttgcg
    43981 ccagcaggtg acggtacgct gaaaggttcc ggtcgctcca ttcaggggct gcatatgcgt
    44041 gatgcgctgg agcgattaga cacactctac cccggcatga tgctcaagtt tggcggtcat
    44101 gcgatggcgg cgggtttgtc gctggaagag gataaattcg aactctttca acaacggttt
    44161 ggcgaactgg ttactgagtg gctggcccct tcgctattgc aaggcgaagt ggtgtcagac
    44221 ggtccgttaa gcccggccga aatgaccatg gaagtggcgc agctgctgcg cgatgctggc
    44281 ccgtgggggc agatgttccc ggagccgctg tttgacggtc atttccgtct gctgcaacag
    44341 cggctggtgg gcgaacgtca tttgaaggtg atggtcgaac cggtcggcgg cggtccactg
    44401 ctggatggta ttgcttttaa tgtcgatacc gctctctggc cggataacgg cgtgcgcgaa
    44461 gtgcaactgg cttataagct cgatatcaac gagtttcgcg gcaaccgcag cctgcaaatt
    44521 atcatcgaca atatctggcc aatttagcgt catcttctct ataaaaaaga gcgtggattg
    44581 ggtacaatcc cgctcttatc accgcatttt gactagctca ataaaagaaa tcagaccatg
    44641 tttgaaatta atccggtaaa taatcgcatt caggacctca cggaacgctc cgacgttctt
    44701 agggggtatc tttgactacg acgccaagaa agagcgtctg gaagaagtaa acgccgagct
    44761 ggaacagccg gatgtctgga acgaacccga acgcgcacag gcgctgggta aagagcgttc
    44821 ctccctcgaa gccgttgtcg acaccctcga ccaaatgaaa caggggctgg aagatgtttc
    44881 tggtctgctg gaactggctg tagaagctga cgacgaagaa acctttaacg aagccgttgc
    44941 tgaactcgac gccctggaag aaaaactggc gcagcttgag ttccgccgta tgttctctgg
    45001 cgaatatgac agcgccgact gttacctcga tattcaggcg gggtctggcg gtacggaagc
    45061 acaggactgg gcgagcatgc ttgagcgtat gtatctgcgc tgggcagaat cgcgtggttt
    45121 caaaactgaa atcatcgaag agtcggaagg tgaagtggcg ggtattaaat ccgtgacgat
    45181 caaaatctcc ggcgattacg cttacggctg gctgcgtaca gaaactggcg ttcaccgcct
    45241 ggtgcgtaaa agcccgtttg actccggcgg tcgtcgccac acgtcgttca gctctgcgtt
    45301 tgtttatccg gaagttgatg atgatattga tatcgaaatc aacccggcgg atctgcgcat
    45361 tgacgtttat cgcgcgtccg gcgcgggcgg tcagcacgtt aaccgtaccg aatctgcggt
    45421 gcgtattacc cacatcccga ccgggatcgt gacccagtgc cagaacgacc gttcccagca
    45481 caagaacaaa gatcaggcca tgaagcagat gaaagcgaag ctttatgaac tggagatgca
    45541 gaagaaaaat gccgagaaac aggcgatgga agataacaaa tctgacatcg gctggggcag
    45601 ccagattcgt tcttatgtcc ttgatgactc ccgcattaaa gatctgcgca ccggggtaga
    45661 aacccgcaac acgcaggccg tgctggacgg cagcctggat caatttatcg aagcaagttt
    45721 gaaagcaggg ttatgaggaa ccaacatgtc tgaacaacac gcacagggcg ctgacgcggt
    45781 agtcgatctt aacaatgaac tgaaaacgcg tcgtgagaag ctggcgaacc tgcgtgagca
    45841 gggaattgcc ttcccgaacg atttccgtcg cgatcatacc tctgaccaat tgcacgcaga
    45901 attcgacggt aaagagaacg aagaactgga agcgctgaac atcgaagtcg ccgttgctgg
    45961 ccgcatgatg acccgccgta ttatgggtaa agcgtctttc gttaccctgc aggatgtggg
    46021 tggccgcatt cagctgtacg ttgcccgtga cgatctgccg gaaggcgttt acaacgagca
    46081 gttcaaaaaa tgggacctcg gcgacatcct cggcgcgaaa ggtaaactgt tcaaaaccaa
    46141 aaccggcgaa ctgtctatcc actgtaccga gctgcgtctg ctgaccaaag cactgcgtcc
    46201 gctgccggat aaattccacg gcttgcagga tcaggaagcg cgctatcgtc agcgttatct
    46261 ggatctcatc tctaacgatg aatcccgtaa cacctttaaa gtacgctcgc agatcctctc
    46321 tggtattcgc cagttcatgg tgaaccgcgg ctttatggaa gttgaaacgc cgatgatgca
    46381 ggtgatccca ggcggtgccg ctgcgcgtcc gtttatcacc caccataacg cgctggatct
    46441 cgacatgtac ctgcgtatcg cgccggaact gtacctcaag cgtctggtgg tcggtggctt
    46501 cgagcgtgta ttcgaaatca accgtaactt ccgtaacgaa ggtatttccg tacgtcataa
    46561 cccagagttc accatgatgg aactctacat ggcttacgca gattacaaag atctgatcga
    46621 gctgaccgaa tcgctgttcc gtactctggc acaggatatt ctcggtaaga cggaagtgac
    46681 ctacggcgac gtgacgctgg acttcggtaa accgttcgaa aaactgacca tgcgtgaagc
    46741 gatcaagaaa tatcgcccgg aaaccgacat ggcggatctg gataacttcg actctgcgaa
    46801 agcgattgct gaatctatcg gtatccacgt tgagaagagc tggggtctgg gccgtatcgt
    46861 taccgagatc ttcgaagaag tggcagaagc acatctgatt cagccgacct tcattactga
    46921 atatccggca gaagtttctc cgctggcgcg tcgtaacgac attaacccgg aaatcacaga
    46981 ccgctttgag ttcttcatcg gtggtcgtga aatcggtaac ggctttagcg agctgaatga
    47041 cgcggaagat caggcgcaac gcttcctgga tcaggttgcc gcgaaagacg caggtgacga
    47101 cgaagcgatg ttctacgatg aagattacgt taccgcactg gaacatggct taccgccgac
    47161 agcaggtctg gggattggca tcgaccgtat ggtaatgctg ttcaccaaca gccataccat
    47221 ccgcgacgtt attctgttcc cggcgatgcg tccggtgaaa taagcattac gttatgctca
    47281 caaccccggc aaatgtcggg gttttttatt taagctgggt aaatgcagat aatcgtattc
    47341 tggcttcgcg atttgtcgcc tgcatcacca tccacggact gaacgcccac ggcgtggcat
    47401 caataccgcg taatacatca gctaaatcac accattgata atccatcact tcatcatcgt
    47461 tgatctgtaa cgcactggtg gtgcgtgcgg caaataccgg acacacttca ttttccacaa
    47521 tgcctctcgg atcggtggcg cggtagcgaa agtcaggata gatagattca ggaggcgtaa
    47581 tttccacgcc aagctcataa cggcaacggc ggatcaccgc gtcttcgctg ctttctccca
    47641 gttgtgggtg cccacaaacc gagttagtcc acacgccagg ccacgctttt ttgctcagtg
    47701 cgcggcgggt aactaataat tgccccttgg cattaaacag ccaactggag aacgcgagat
    47761 gtaagagggt gtctgccgtg tgtgcggcat acttttccag cgtacccgtg ggaactccct
    47821 gtgcattcaa taaaatgacg tgttccgttt gcataatttc tcacatgtaa ttctgatcac
    47881 tcatcattat agataattga agagtgaaat gattaaccaa gagagcgttt atcgacaacc
    47941 gcccgttttg agcggatgcc gacaggtaaa aaccatttaa aaaagtagca tcatatattt
    48001 gaagcgactt tcaatggcca ttgcaacaca gtcagaaatg acaggaatag agagagtaaa
    48061 aagcaacaac agactgaata tagcgcgcat taaaaaattc atattattct ccatgctcat
    48121 ttttcttcag cttgcgcatc agaagccaca tacgtactgt cctgacttct aaatctttat
    48181 cagagacgga ttccttcacc aggtcagcat gatgctgata gccattaaag aagacgttca
    48241 gcacaacggc gctaagggtt gcgagcataa tgccgctatg tagcagcggt tgcagtacgg
    48301 ccggtaactt agaaaagaaa tcgtgagaga gcgtcggagt catgccaacg ccgagactga
    48361 tcgccacaat atagaggttg taacggttgg tggtgtagtt acagcgcgac agaattcgaa
    48421 tccctgtcgc cagtaccatg ccgaacatca ctagaccagc gccgcccagc acaaattgcg
    48481 gaatggaggc taccagcacc gccatttttg gcaccatgcc aaacaggatt aatataattc
    48541 ccgaagaaat acacacccag cggctatgaa cgcgcatcac gctaaccagg ccaacgtttt
    48601 gagaaaatga cgtgtggggg aagctgttaa acgtgccgcc tatcattgtc cctacgccat
    48661 cgacacgcag cccgcaaata atatcgtgtg aagagagttt acgaccgact atttcaccca
    48721 gtgccaggaa catccccatc gactcgataa acacgatgat taacacggca gtcatggtca
    48781 gaatggaaac gggatcgaaa atcggcatac caaacgacat cggggtgaca atcgcaaacc
    48841 acgaggcatc atgtagcccg gataaattga cttcattcat catccacgaa agtaaaaagc
    48901 caaatacaat ccccagtaat acggcgacgt tggacataaa tcctttcgca tagcgagtaa
    48961 tgagcaagat aaaaattaag acggcaaagg agatacctaa ataaacggga ttaccatatt
    49021 gcggattccc tttacctcca gcggcccagt caatacccac ctgaatgatg ctaagcccaa
    49081 tagaagtaat aaccacaccg gtaaccagtg gcgggaataa aggcatcaag cgaccaataa
    49141 gtggcgctaa taatgtggtg ataaaacccg cggcgatagt ggcaccaaat atccccagca
    49201 ggccgatatc cgggttcatc ccaatggcta tcattggtgt tacagcagca aaggtcaccg
    49261 acataatcac cggcaggcgg atccccataa agcggccgat accgatacat tgcaataatg
    49321 tgacgatccc gcagcaaaag agatccgagc taatgagcat cgcaatagca tcttttgaga
    49381 ggcccagtcg gtcaccaatc ataagaggaa cagcgactgc acctgcgtac atgacaagga
    49441 cgtgttgcag accgagtatg atcagctttc ctggtgataa tatgcggtca acctcatcag
    49501 ttgggcggtc ttgcccagaa gatgagggaa gttgggaatc tatggcgctc atcagtgttt
    49561 ctcctttcgc ccgccagaat aaaaagcaac cgtttacgtt aatgaaactg tctaaacaca
    49621 tgctcgtgga gtgccagttt ttgcgaggaa ggggagttag agacaggaat tgcggaggca
    49681 atcacataat cgtgcttaaa ccacgaaaat gacaagagaa actgacaatg gaattatctg
    49741 taattttctc atattgatat ttcattaatc attctggcat gtcgcactct cgcatttaat
    49801 cgtttttatc tggatagcgc tcttttgatc ggcaatgttg aaataatgaa agcggcatag
    49861 attgaggggc aataatttga actcagtcgc aaaattataa ttaaaacggt catgctacct
    49921 aatatatgcc cgtttattgc atcgcgaaat ttgagaagac ataactcatt aaatgaaagg
    49981 gatgcaaatg aatacgttta tcgctgctga agctgcggaa tgtataggct gtca
//
LOCUS       DAETQL010000022        49899 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.022, whole genome shotgun sequence.
ACCESSION   DAETQL010000022 DAETQL010000000
VERSION     DAETQL010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.022"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..126
                     /locus_tag="K0W65_001369"
     CDS             <1..126
                     /locus_tag="K0W65_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3094693.1"
                     /translation="RHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(120..1298)
                     /gene="setB"
                     /locus_tag="K0W65_001370"
     CDS             complement(120..1298)
                     /gene="setB"
                     /locus_tag="K0W65_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBI3094694.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFWVMTPTY
                     "
     gene            1666..2796
                     /gene="fruB"
                     /locus_tag="K0W65_001371"
     CDS             1666..2796
                     /gene="fruB"
                     /locus_tag="K0W65_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBI3094695.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2796..3734
                     /gene="fruK"
                     /locus_tag="K0W65_001372"
     CDS             2796..3734
                     /gene="fruK"
                     /locus_tag="K0W65_001372"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBI3094696.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3751..5442
                     /gene="fruA"
                     /locus_tag="K0W65_001373"
     CDS             3751..5442
                     /gene="fruA"
                     /locus_tag="K0W65_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBI3094697.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5866..6807
                     /locus_tag="K0W65_001374"
     CDS             5866..6807
                     /locus_tag="K0W65_001374"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBI3094698.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6795..7733
                     /gene="psuG"
                     /locus_tag="K0W65_001375"
     CDS             6795..7733
                     /gene="psuG"
                     /locus_tag="K0W65_001375"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBI3094699.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7827..9077
                     /locus_tag="K0W65_001376"
     CDS             7827..9077
                     /locus_tag="K0W65_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBI3094700.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9148..9842)
                     /gene="yeiL"
                     /locus_tag="K0W65_001377"
                     /pseudo
     CDS             complement(9148..9842)
                     /gene="yeiL"
                     /locus_tag="K0W65_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9973..10914
                     /gene="rihB"
                     /locus_tag="K0W65_001378"
     CDS             9973..10914
                     /gene="rihB"
                     /locus_tag="K0W65_001378"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HBI3094701.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            11014..12264
                     /locus_tag="K0W65_001379"
     CDS             11014..12264
                     /locus_tag="K0W65_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBI3094702.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12320..13408)
                     /locus_tag="K0W65_001380"
     CDS             complement(12320..13408)
                     /locus_tag="K0W65_001380"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBI3094703.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13411..14268)
                     /gene="nfo"
                     /locus_tag="K0W65_001381"
     CDS             complement(13411..14268)
                     /gene="nfo"
                     /locus_tag="K0W65_001381"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBI3094704.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14342..15391)
                     /locus_tag="K0W65_001382"
     CDS             complement(14342..15391)
                     /locus_tag="K0W65_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBI3094705.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15490..16371
                     /locus_tag="K0W65_001383"
     CDS             15490..16371
                     /locus_tag="K0W65_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3094706.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16577..18046
                     /gene="lysP"
                     /locus_tag="K0W65_001384"
     CDS             16577..18046
                     /gene="lysP"
                     /locus_tag="K0W65_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBI3094707.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18338..20329
                     /gene="cirA"
                     /locus_tag="K0W65_001385"
                     /pseudo
     CDS             18338..20329
                     /gene="cirA"
                     /locus_tag="K0W65_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20361..21196)
                     /gene="yeiG"
                     /locus_tag="K0W65_001386"
                     /pseudo
     CDS             complement(20361..21196)
                     /gene="yeiG"
                     /locus_tag="K0W65_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21454..22122
                     /gene="folE"
                     /locus_tag="K0W65_001387"
     CDS             21454..22122
                     /gene="folE"
                     /locus_tag="K0W65_001387"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBI3094708.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            22139..23215
                     /locus_tag="K0W65_001388"
     CDS             22139..23215
                     /locus_tag="K0W65_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBI3094709.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23357..24397
                     /gene="galS"
                     /locus_tag="K0W65_001389"
     CDS             23357..24397
                     /gene="galS"
                     /locus_tag="K0W65_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HBI3094710.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24677..25675
                     /gene="mglB"
                     /locus_tag="K0W65_001390"
     CDS             24677..25675
                     /gene="mglB"
                     /locus_tag="K0W65_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBI3094711.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25736..27256
                     /gene="mglA"
                     /locus_tag="K0W65_001391"
     CDS             25736..27256
                     /gene="mglA"
                     /locus_tag="K0W65_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBI3094712.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27272..28282
                     /gene="mglC"
                     /locus_tag="K0W65_001392"
     CDS             27272..28282
                     /gene="mglC"
                     /locus_tag="K0W65_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBI3094713.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28318..29531)
                     /gene="preA"
                     /locus_tag="K0W65_001393"
                     /pseudo
     CDS             complement(28318..29531)
                     /gene="preA"
                     /locus_tag="K0W65_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29525..30763)
                     /gene="preT"
                     /locus_tag="K0W65_001394"
     CDS             complement(29525..30763)
                     /gene="preT"
                     /locus_tag="K0W65_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HBI3094714.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30957..31196)
                     /locus_tag="K0W65_001395"
     CDS             complement(30957..31196)
                     /locus_tag="K0W65_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBI3094715.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31199..31918)
                     /gene="sanA"
                     /locus_tag="K0W65_001396"
     CDS             complement(31199..31918)
                     /gene="sanA"
                     /locus_tag="K0W65_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBI3094716.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32068..32952)
                     /gene="cdd"
                     /locus_tag="K0W65_001397"
     CDS             complement(32068..32952)
                     /gene="cdd"
                     /locus_tag="K0W65_001397"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBI3094717.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33082..33777)
                     /locus_tag="K0W65_001398"
     CDS             complement(33082..33777)
                     /locus_tag="K0W65_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBI3094718.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33774..34172)
                     /locus_tag="K0W65_001399"
     CDS             complement(33774..34172)
                     /locus_tag="K0W65_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBI3094719.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34411..35358
                     /gene="dusC"
                     /locus_tag="K0W65_001400"
     CDS             34411..35358
                     /gene="dusC"
                     /locus_tag="K0W65_001400"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBI3094720.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35729..35812)
                     /gene="yohP"
                     /locus_tag="K0W65_001401"
     CDS             complement(35729..35812)
                     /gene="yohP"
                     /locus_tag="K0W65_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBI3094721.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            36036..37472
                     /gene="mdtQ"
                     /locus_tag="K0W65_001402"
                     /pseudo
     CDS             36036..37472
                     /gene="mdtQ"
                     /locus_tag="K0W65_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37525..38286
                     /locus_tag="K0W65_001403"
                     /pseudo
     CDS             37525..38286
                     /locus_tag="K0W65_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38403..38981)
                     /locus_tag="K0W65_001404"
     CDS             complement(38403..38981)
                     /locus_tag="K0W65_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI3094722.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39151..39738
                     /locus_tag="K0W65_001405"
     CDS             39151..39738
                     /locus_tag="K0W65_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBI3094723.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39912..40844
                     /gene="pbpG"
                     /locus_tag="K0W65_001406"
     CDS             39912..40844
                     /gene="pbpG"
                     /locus_tag="K0W65_001406"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBI3094724.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40882..42597)
                     /gene="dld"
                     /locus_tag="K0W65_001407"
     CDS             complement(40882..42597)
                     /gene="dld"
                     /locus_tag="K0W65_001407"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBI3094725.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42793..45090
                     /gene="bglX"
                     /locus_tag="K0W65_001408"
     CDS             42793..45090
                     /gene="bglX"
                     /locus_tag="K0W65_001408"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBI3094726.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45342..46259
                     /gene="osmF"
                     /locus_tag="K0W65_001409"
     CDS             45342..46259
                     /gene="osmF"
                     /locus_tag="K0W65_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBI3094727.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46266..47423
                     /gene="yehY"
                     /locus_tag="K0W65_001410"
     CDS             46266..47423
                     /gene="yehY"
                     /locus_tag="K0W65_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HBI3094728.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47416..48342
                     /gene="yehX"
                     /locus_tag="K0W65_001411"
     CDS             47416..48342
                     /gene="yehX"
                     /locus_tag="K0W65_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HBI3094729.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48347..49078
                     /gene="yehW"
                     /locus_tag="K0W65_001412"
     CDS             48347..49078
                     /gene="yehW"
                     /locus_tag="K0W65_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBI3094730.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(49059..49166)
                     /locus_tag="K0W65_001413"
     CDS             complement(49059..49166)
                     /locus_tag="K0W65_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBI3094731.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49226..49682)
                     /locus_tag="K0W65_001414"
                     /pseudo
     CDS             complement(49226..49682)
                     /locus_tag="K0W65_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(49724..>49899)
                     /locus_tag="K0W65_001415"
     CDS             complement(49724..>49899)
                     /locus_tag="K0W65_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3094732.1"
                     /translation="EVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHIPPAEAEK
                     AYYASIGNDDLAA"
BASE COUNT        12043 a        12299 c        13399 g        12158 t
ORIGIN      
        1 cgacataatc tgaatctgag acaacatctg gcaaggctgg gacggaagtc actgtcgttc
       61 tcaaaatcgg tggagctgca tgacaaggtc atcgggcatt atctgaacat aaaacactat
      121 cagtaagttg gagtcattac ccaaaacaga gtggcgataa tcatcaccat cgcaaaccag
      181 aacacagcgt gataattcca gatctcggcg acgatgcccg ccactgatcc tgcgatgatc
      241 cagcccacgc gcgacgtgtt ggtatagagc gtggtggctg aacctgcctg accgggcatc
      301 aaatcctgaa aatagagcat cccgatgccg cccagaatgc caataaaaat agcatttagc
      361 agctgcaagc ccaacagaat ggcaggtgaa tgcgccatca gcattcctgc gtaaaaacag
      421 acgccaccca cggcagcaac gcgcattaag aaacgcttac ccagacgttt ggcgaaatat
      481 ccggcaatca acatcgtcgg gatttccagc ccggcggcgg tccccatcat cacaccggcc
      541 agtttctcgg gaagatgcag ttcgttgata ataaatagcg gcatgttgat gatgtacagg
      601 ctgttcgagc cccacatcaa tgtacaaatg acaaacagca gtagcgtatc gcgacggtta
      661 cggcgcggcg cttcaaccgt gccggtcgcc agcggaagct ctttttgcat cgacggtaaa
      721 aacagccaca ccatcacgcc gcaaacaata aacgctaccg ctgcgctcag atacattacc
      781 gtaaagctga aacccatcgc taacgcataa gccagcggtg ggccaatgac ccatgccagt
      841 gaaacctgag cgcgtaaaaa agagctgaac atcaccgcct cacgtccggt tttgtcggca
      901 tgttcacggg caagggcaaa catttgcggg ttagcggtcg agccaaagct gctaagaaag
      961 acgccaacga atagcaaaac aaagtagttg cgattccaag caaaaagggt gcaggccagc
     1021 acgcctaaca ggcagcaaaa gacaatcagc gatttgcgat cgccgcgctt atcagagcgc
     1081 ccagcgagaa actgacttac cagaatccca atgacagcgc tgccggtaaa gaagaatccc
     1141 accatcgccg gacgggcatg tacttcatcg gtaaggaaaa tactgagtgt cggggtttgc
     1201 agagcacccg caataccggt gagaaaggca acgattaaaa acgccgtcga ggtcaggtca
     1261 aacgatttcg cgctggagac tgcgggggag ttatgcatgc tggtcgatca ctggaagagg
     1321 aagacgcgga gtttacgctg cttgtcagaa aataaacaga cagcgaaatc aaaagcgcca
     1381 cgaataatca aaatgccatt tcactgttgc tgattggctg ctgacgctgc tgaaatgaaa
     1441 ttgctgataa cgaattttcc catcagcaat taggaaaaat gacaaaaaat tgtgcagcac
     1501 atcaaacttt tgctcataac tttacggctt tccttgcgtg ctgaaacaga agtattatgc
     1561 tttcttgaaa cgtttcagcg cgatcttgtc tttaacccta agctaggttg gcgctttttt
     1621 tctcatagag gctgaatcgt ttcaattcag caagagagga gaactatgtt ccagttatcc
     1681 gtacaggaca tccatccggg cgaaaaggcc ggagacaaag aagaggcgat tcgccaggtc
     1741 gctgcggcgc tggtgcaggc cggtaatgta gcagaaggct acgtcaatgg catgctggcg
     1801 cgcgaacagc aaacctcaac gttcctcggc aatggtattg ctattccaca cggcactacc
     1861 gacacccgcg atcaggtgct gaaaaccggc gttcaggtat ttcagttccc ggaaggcgtc
     1921 acctggggtg acggtcaggt agcgtacgtg gcaatcggta ttgctgccag ctcggataaa
     1981 catctgggcc tgctacgcca gctgacccac gtactgagcg atgattccgt tgctgaacaa
     2041 ctgaagtcag caacaacagc agaagaactt cgcgcattac tgatgggcga aaagcagagt
     2101 gagcagctga agctcgacaa cgaaatgctg acgctggata tcgtcgccag cgatctgctg
     2161 actcttcagg cgctgaacgc tgcgcgtctg aaagaggcga gggcagttga cgccactttc
     2221 gtcaccaaag ccatcaatga acaaccgctg aatctcggac agggtatctg gctgagcgat
     2281 agcgccgaag gcaatctgcg tagtgcgatt gcggtaagcc gtgcggcaaa tgcttttgat
     2341 gtggacggcg aaacggcagc catgctggtg agtgtggcga tgaatgacga tcagcccatc
     2401 gcggttctta agcgtctcgc tgatttgttg ctcgacaata aagctgaccg cttgctgaaa
     2461 gcggatgcgg caacgttgct ggcgctgctg accagcgatg atgcgccgac cgacgacgtg
     2521 ttaagcgcgg agtttgtggt gcgcaatgaa cacggcctgc atgctcgtcc aggtaccatg
     2581 ctggtcaata ccattaaaca atttaacagt gatattaccg tgacaaacct tgatggcacc
     2641 ggcaaaccgg caaacggacg tagtctgatg aaagttgtgg cacttggcgt taagaaaggt
     2701 catcgcctac gctttaccgc ccagggtgca gatgctgaac aggcgctgaa agccattggc
     2761 gacgctatcg ctgctggtct tggggagggc gcataatgag cagacgtgtt gctacgatca
     2821 cccttaatcc ggcttatgac cttgttggtt tctgcccgga aattgaacgc ggcgaagtga
     2881 acctggtgaa aaccaccggt ctgcatgcgg cgggtaaagg catcaacgtg gccaaagtat
     2941 taaaagacct gggaattgat gtcaccgttg gcggcttcct gggtaaagac aatcaggatg
     3001 gttttcagca actgttcagc gagctgggca ttgccaaccg tttccaggtt gtacaggggc
     3061 gcacccgaat taacgttaag ctgacggaaa aagacggcga agtgaccgac ttcaacttct
     3121 cgggtttcga agtcaccccc gccgactggg aacgctttgt gactgattct ctgagctggc
     3181 tcggtcagtt cgatatggtc tgtgtcagcg gaagcttacc gtcaggcgtc agcccggaag
     3241 cgttcaccga ctggatgact cgcctgcgta gtcagtgtcc ttgcattatc tttgatagta
     3301 gccgtgaagc gttagtagca ggtttgaaag cggcaccgtg gctggtgaaa cctaaccgcc
     3361 gcgagttgga aatctgggca ggccgtaaac tgcctgaaat gaaagatgtg attgaagctg
     3421 cacatgcgct acgtgaacaa ggtattgcgc atgttgttat ttcactgggt gccgaaggcg
     3481 cgctttgggt taatgcctcc ggcgaatgga tcgctaaacc accgtcagtc gatgtcgtaa
     3541 gcaccgttgg cgcaggggat tctatggttg gtggcctgat ttatggcttg ctgatgcgtg
     3601 aatccagtga acacacactg cgtctggcga cagctgttgc agccctggcg gtaagtcaaa
     3661 gcaatgtggg tattaccgat cgtccgcagt tggccgcaat gatggcgcgc gtcgacttac
     3721 aaccttttaa ctgacagcag gagaggcata atgaaaacgc tgctgattat tgacgctaat
     3781 ctcggtcagg cacgcgccta tatggcgaag accctgctgg gcgcggcggc gcgaaaagca
     3841 aaactggaaa tcatcgacaa tccgaacgac gcagaaatgg cgattgttct cggtgattcc
     3901 atcccgaatg acagcgcgct gaacggtaaa aatgtctggc tgggtgatat ttcccgggca
     3961 gttgcgcacc ctgagctgtt cctgagtgaa gccaaaggcc atgcgaaacc ttacactgcg
     4021 ccggtcactg cgacagcacc ggttgccgcc agcggtccga aacgcgtagt tgcggtgact
     4081 gcttgcccga ctggcgtagc acacaccttt atggcggctg aagccattga aaccgaagcg
     4141 aaaaaacgtg gctggtgggt gaaagttgaa acccgtggtt ctgttggcgc gggtaatgca
     4201 atcactcccg aggaagtagc cgcagcggat ctggtgattg tggcggcaga tatcgaagtg
     4261 gatctggcga aatttgctgg taaaccgatg tatcgtacct ctaccggtct ggcgctgaag
     4321 aaaacctcgc aggaactgga taaagcggtt gctgaagcaa cgccgtatga accggcgggc
     4381 aaagctcaaa cggcgaccac tgaaggtaag aaagagagtg caggcgctta tcgtcacttg
     4441 ctaacgggcg tctcttacat gctgccgatg gtcgttgctg gtggtctgtg tatcgcgctt
     4501 tcttttgctt ttggtatcga agcgtttaaa gagccgggta cgttggcagc ggcgctgatg
     4561 cagattggtg gtggttcagc ctttgcgctg atggtgccgg tactggcagg ttatattgcc
     4621 ttctccattg ccgatcgtcc gggcctcacg ccgggtctga ttggcggtat gctggcggtc
     4681 agcaccggtt ctggcttcat tggcggtatt attgcgggct tcctggctgg ttacattgcg
     4741 aagttaatca gtacgcaact gaaactgccg cagagtatgg aggcgctgaa accgatcctg
     4801 atcattccgc taatttccag tctggtggtc ggtctggcga tgatctacct gatcggtaaa
     4861 ccagttgctg gcattctcga agggctgact cactggctgc agaccatggg gactgcgaat
     4921 gcggttctgc tgggggcgat cctcggtggc atgatgtgta ctgacatggg cggtccggta
     4981 aacaaagcag cgtacgcatt cggtgtgggt ctgctgagta ctcaaaccta tggcccgatg
     5041 gcggcgatta tggcggcagg tatggtgcca ccgctggcaa tgggcctggc aacaatggtg
     5101 gcgcgtcgca aattcgacaa agcgcagcag gaaggtggca aagccgctct ggtattggga
     5161 ctgtgcttca tttcggaagg tgcaattccg tttgctgctc gtgatccgat gcgtgtgctg
     5221 ccgtgctgta tcgtgggtgg ggcgctgact ggcgcaatct caatggcgat tggtgcgaaa
     5281 ctgatggcac cgcacggtgg tctgtttgtt ctgctgatcc ctggcgctat tacgccggta
     5341 ttgggttacc tggtagcaat tattgccggt acgctggtgg cgggtttggc ctatgccttc
     5401 ctgaaacgtc cggaagtgga cgcagtagcg aaagcagcgt aataaaaggt gtgttacagg
     5461 gcagaaattt cctgccctgt aacatttgat gagcgcaaag agaatgacca gcttgtgaat
     5521 gtaattttat gaatttaaat ggttttatct ttcagtagaa acttcaaacg ttcatcaaac
     5581 actaaggtta tttcctcaag ggcagattat tttctgccct tttttagtgt tcctgttctg
     5641 tgcatcccgt cacaaattcc tcgcttttgt aattcaggca tttaatcgca ttttgtgagc
     5701 ctgctcaaat ctgcccattt tgcctctctg gcggtaattt actttacgta aaacatgcat
     5761 actatgagca catgtttaaa tatgaacaca tgtttaaacc gtctttgcag tatctatatt
     5821 cttgtggatt gacagggttg gtaatttttt accagaggac gtgctatgcg cgaaaaggat
     5881 tatgtcgtaa ttataggttc ggcgaatatt gatgtcgccg gatattcaca tgaatcatta
     5941 aattatgcgg attcaaatcc aggtaaaata aaatttacgc ctggtggagt agggcgcaat
     6001 attgcacaaa acctggcgtt gctgggtaac aaagcctggc tactgagcgc cgtaggcagt
     6061 gatttttatg gtcaatcgct gctaacgcaa accaatcaat ctggtgttta tgtcgataaa
     6121 tgcctgattg tgccgggaga aaatacgtcg agttatttat cattactcga taataccggt
     6181 gaaatgctgg ttgctataaa tgacatgaat attagcaacg ctattacagc tgaatatctc
     6241 gcacagcacc gtgaatttat tcagagggca aaggtcattg tcgcggactg taatatcagt
     6301 gaagaggcac tggcatggat tctggataat gccgccaacg tacccgtatt tgtcgatccg
     6361 gtttcagcgt ggaaatgtgt caaagtgcgc gaccgtctaa atcagaacca cactctgaag
     6421 ccaaaccgcc ttgaagcgga aaccctgagt gggattgcgc tgtcagggcg tgacgatgtg
     6481 gcaaaagttg ctgcctggtt ccatcaacat ggcctgaacc gactggtatt gagcatgggc
     6541 ggcgacggcg tttattacag cgatatcagc ggtgaaaatg gctggtctgc gccgatcaaa
     6601 accaatgtta ttaatgttac cggagcgggc gatgccatga tggcgggact tgcttcgtgt
     6661 tgggtagacg gaatgccgtt tgccgaatct gttcgtttcg cacagggatg ttcgtcaatg
     6721 gcgctctcct gtgaatacac caataacccc gatttatcga ttgccaacgt tatatcgtta
     6781 gtggagaacg cagaatgtct gaattaaaaa tttcccctga attattacaa atttctccgg
     6841 aagtgcagga cgctttaaaa aacaaaaaac cggttgtggc gctggaatcg accattattt
     6901 ctcacgggat gccgttccca caaaatgccc agaccgcaat tgaagtagaa gaaactattc
     6961 gtaaacaggg cgcagtacct gccactatcg ccattattgg cggcgtgatg aaagtgggtt
     7021 taagcaaaga aaaaattgaa ttactgggtc gtgaagggca taacgtgacc aaagttagtc
     7081 gtcgcgattt accttttgtt gttgccgccg gaaaaaatgg cgcaaccact gtggcttcaa
     7141 cgatgattat tgcggcgctt gccggaatta aagtatttgc caccggggga attggtggtg
     7201 tgcatcgcgg ggcggaacat accttcgata tttctgccga tttgcaagaa ctggcaaata
     7261 ctaatgtcac cgttgtttgt gccggggcga aatctattct cgatttagga ttaaccactg
     7321 agtatttaga aaccttcggt gtgccgttaa ttggctatca gactaaagcg ctgcctgcgt
     7381 ttttctgccg taccagctcg tttgacgtca gcattcgtct cgacagcgcc agtgaaattg
     7441 cccgtgcaat ggcggtgaaa tggcaaagcg ggctgaacgg tggcctcgtg gtagcgaacc
     7501 cgatcccgga acagtttgcg atgccggagg aatctatcaa tgcagccata gatcaagccg
     7561 tcgccgaagc cgaagagcag ggcgttattg gtaaagaaag tacaccgttc ctgctggcgc
     7621 gcgttgctga gctgaccagc ggtgacagcc tgaaatccaa tatccagctg gtgttcaaca
     7681 acgccattct ggcgagtgaa attgccaaag aataccagcg tctcgcgggt taatttctga
     7741 cgggcagaaa gccgtcgccc gtactcatca agcataccaa aacctggcgc tgacgccggg
     7801 tttactggca tgaagggaaa atcattatgg atataatgag aagtgttgtg gggatggtgg
     7861 tgttactggc aatagcattt ctgttgtcag tgaataaaaa gagcatcagt ttgcgcacgg
     7921 ttggagccgc actgctgctg caaatcgcta ttggtggcat catgctctac ttcccaccgg
     7981 gaaaatgggc agtagaacag gcggcattag gcgttcataa agtgatgtct tacagtgatg
     8041 ccggtagcgc cttcattttt ggttcgctgg ttgggccgaa aatggatgtc ctgtttgacg
     8101 gtgcgggttt tatcttcgcc tttcgcgtac ttccggcgat tattttcgtt actgcgctca
     8161 tcagtctgct gtactacatt ggcgtgatgg ggctgctgat tcgcatcctt ggcagcattt
     8221 tccagaaagc cctcaacatc agcaaaatcg aatcttttgt tgcggttacc actattttcc
     8281 tcgggcaaaa tgagatcccg gcgatcgtta aaccgtttat cgatcgcgtg aatcgcaacg
     8341 agttgtttac cgcaatttgt agcgggatgg cgtccattgc tggttcggtg atgattggtt
     8401 atgccggaat gggcgtacca attgactacc tgttagcggc atcgctgatg gcgatccccg
     8461 gcggtatttt gtttgcacgt attcttagcc cggcaaccga gccttcgcag gtcacatttg
     8521 aaaatctgtc gttcagcgaa acgccgccaa aaagctttat cgaagcggcg gcgagcggtg
     8581 cgatgaccgg gctgaaaatc gccgctggtg tagcgacggt ggtaatggcg tttgtcgcaa
     8641 ttattgcgct gatcaacggc attatcggcg gaattggcgg ctggtttggt ttcgccaatg
     8701 cctctctgga aagtattttt ggctatgtgc tggcaccgct ggcgtggatc atgggtgtgg
     8761 actggagtga tgccaacctt gctggtagcc tgattgggca gaaactggcg attaacgaat
     8821 tcgtcgctta cctgagtttc tccccatacc tgcaaacggg cggcacgctg gaagtgaaaa
     8881 ccgtggcgat tatatccttt gcgctttgtg gttttgctaa ctttggttct atcggtgttg
     8941 tcgttggcgc attttcggct atttcgccaa aacgtgctcc agaaatcgcc cagcttggtt
     9001 tacgggcgct ggcagcagca acgctttcca acctgatgag tgcgactatt gccgggttct
     9061 ttattggtct ggcgtaatgc tcgatttgta gaccggataa aacgtattaa gcgttgcatc
     9121 cggcaatcgc gctattgcaa ataattttta ctgcatcatc ccggagaatt tattctccgg
     9181 gtccatctcc agcgccagtc ctgacaactg ctttctgttt ttaattagat atcctttctt
     9241 ttaattaata aaccgtcgtg aatgaattgc gcgagaacat ataaaagatg tcgataagaa
     9301 acgcctaaat actctgccgc ttgcgtatgc ttttcgtgat aaagatcacc ttcttgtgag
     9361 agtaatataa atgctgccag gcgattcact aacggaaatg attgattctg agttaaagaa
     9421 acaatattac gataattttt atgacttaag gtgacgcaaa gttttcgtaa aaatagggtg
     9481 tcgtttaata acagcggacg gtaatgtttc atagggagcg caaggcacca acactcttca
     9541 atagcctgca ccgcacgcgg ttcatggtct ttgtcgatta actcaatctc gccaataaaa
     9601 cagggagcgg caaagaaatc gatcagcgac acgcgaccat tggcaagcgt ggcgtaaagc
     9661 ctggcgcggc ctcgcgtcag ataaaacagc catgacggct gttgcccttc ctgcacaatg
     9721 tagtcacgcg ctaaaaaatg aaacagccgc gtatcggctg aaacatccgt tgaaaagcaa
     9781 tccttaaacg cagattcact catcaattgc tgcttaaggt cattattgtg gatttctttc
     9841 acgatgttta tccgctatga gatttctcat atttagcata cgtacgctgt tgttaagatt
     9901 caatcattgg cccggtcgaa ttttctttgc tgtagaaaaa aataacgcaa ctggaaacag
     9961 aggaaataaa caatggaaaa gagaaaaatt attctggatt gtgagccggg tcatgatgat
    10021 gctattgcaa tgatgatggc agcgaaacat ccggcaatag atttattagg catcactatt
    10081 gtagcgggta atcagacgct tgataaaaca ttaattaatg gcctgaatgt ttgccagaaa
    10141 ctggagatta atgttccggt ttatgcgggg atgccgcaac ccattatgcg taaacaaatc
    10201 gttgccgata atattcacgg tgaaaccgga ctggatggcc cggtattcga gccgctgacc
    10261 cgccaggcag aaagtaccca tgcggtgaaa tatatcatcg ataccctgat ggcaagcgat
    10321 ggcgatatca ctctggtgcc ggttggtccg ctttcaaata tcgcggtggc aatgcgtatg
    10381 caacccgcaa tcctgcccaa aatccgtgaa attgtgctaa tgggcggcgc ttacggtaca
    10441 ggcaacttca ccccatctgc cgagttcaat atctttgccg acccggaagc cgcccgcgtg
    10501 gtgttcacct ccggcgttcc attagtgatg atgggcctcg atcttaccaa ccagaccgtt
    10561 tgcaccccgg atgtaattgc tcggatggaa agggtaggcg gccctgccgg agagctgttc
    10621 agcgacatca tgaacttcac tctcaaaacg cagttcgaat actacggcct ggctggcggc
    10681 ccggtgcatg acgccacctg cattggttat ctgatcaacc ctgatggcat taaaacccag
    10741 gatatgtacg tcgaagtgga cgtcaacagc ggcccttgct acgggcgtac ggtctgcgac
    10801 gaactgggcg ttcttggcaa gcccgctaat accaaagtcg gcatcactat tgataccgac
    10861 tggttctggg gcttagtcga agagtgcgtg cgcggctaca tcaaaaccca ttaagtgttg
    10921 ataacaagcc gggacagtat cgcaagatgc aggcccggct atttctgatg ggcagaaagc
    10981 cgtcgcccaa aatgacatga agagataatc actatggatg tcatgagaag tgttctggga
    11041 atggtggtac tgctggcaat tgcgttttta ctgtcagtaa acaagaagaa gatcagcctg
    11101 cgtaccgttg gcgcggcgtt agtgttacag gtcgtgattg gcgggattat gctttggtta
    11161 ccgccagggc gttgggtcgc agaaaaagtc gcttttggcg tccacaaagt gatggcgtac
    11221 agcgacgcag gtagcgcatt tatcttcggt tctctggtcg gaccgaaaat ggatacctta
    11281 tttgatggcg caggatttat ctttggtttc agggtattac cggcaattat cttcgtcact
    11341 gcactggtga gtattctcta ctacatcggt gtgatgggga tcttaattcg cattctcggc
    11401 ggtatcttcc agaaagcttt aaatatcagc aagatcgagt cattcgtcgc ggtcaccacc
    11461 attttcctcg ggcaaaacga aattccggcg atcgtcaaac cctttatcga tcgtctgaat
    11521 cgcaatgaat tatttacagc gatttgtagt ggcatggcct cgattgctgg ttcgacaatg
    11581 attggttacg ccgcactggg cgtacctgtg gaatatttgc tggcggcatc gttaatggcg
    11641 atccctggcg ggatcttgtt tgcccgcctg ttaagcccgg ctacggaatc ttcgcaggtt
    11701 tcttttaata acctctcttt caccgaaaca ccgccaaaaa gcattattga agccgatgcg
    11761 acaggggcaa tgaccgggct gaaaatcgcc gccggtgtag cgacagttgt tatggcattt
    11821 gtcgccatca ttgcgttaat taacggtatt atcggcggcg ttggcggctg gtttggtttt
    11881 gaacatgcct cgctggagtc cattttaggt tacctgctgg ccccacttgc gtgggtaatg
    11941 ggtgttgact ggagtgatgc aaatcttgcc gggagtttga ttggacagaa actggcaata
    12001 aatgaatttg tcgcttatct caatttctca ccctatctgc aaacggctgg cactctggat
    12061 gctaaaaccg tggcgattat ttccttcgcg ttgtgcggtt tcgctaactt tggttctatc
    12121 ggggtggtgg tgggggcgtt ttctgcggtt gcgccacacc gtgcgccgga aatcgcccag
    12181 cttggtttac gcgcgctggc ggcggcgaca ctttctaatc tgatgagtgc caccattgcc
    12241 gggttcttta ttggtttagc gtagtgtgca atttatgagc cggattagat gcggtcaagc
    12301 gtcgaatccg gcaacattgt tacaccattg gcactaatga cagcgcgtta tcggcagaca
    12361 gggtagggtt gtttaagctg ccgctggcgc gtgaaattgc cgcgcaggcc atcgcaaaac
    12421 gggcgctgtc acggaaattg tttccctcca gaaagctata taccagaccc gccataaaac
    12481 catcgtcagc accaaaactg tctaccgtcg tatgcgctgg cacagtcagc aaaaattgtt
    12541 caccatcctt ttcgctgcaa taaactgact catcgggcaa ataaacaaac agttgctgaa
    12601 caccttgctg atgcaatgca ttcactgcgg cattacggtc agcatcgctg gtgatcgcct
    12661 gtccccataa aatttccagc tccggtaaag tgggtttcag ggtgtgaata tgcgccagcc
    12721 agtgtttgat tttgcccgct ttgaattctg aaacggtatc gacaaacacc gggatctcat
    12781 cagcgagggt aaagacccat tccagcgcct cggctgtcag gttgcaatca gccagtacca
    12841 cgcccgcatg acgaagtaaa tcgcgcgacc cgttcagtag ttgcggtgtc aactgttcca
    12901 gcagatgggt atcgttaatt gccagcacgg tttgatcgtc tcgattggca attgccagat
    12961 acgtcgatgt gctttgacca tgcaaacgaa cgcagccgga gacattcacg ccggcacggc
    13021 gcgtttcttc caggagcatt tcgccataaa agtcatcgcc aatcactgaa agcaaatgga
    13081 cgtcacggcc taacagcgcc agattgtggg cgatattgcg tccaacgccg cctgccgaac
    13141 aatgaattgt accgggatga gaagccgctt gcgggtaatg gatatccgcc atcccgcgaa
    13201 tatccatatt gattgtcccc accactacgc agtattcctg ctcggtgaga atgtaacctt
    13261 tgtctttaat ccggcctttg cgcattaaat ccataatatg cgccgcaacg cgcgaacgac
    13321 tgatttgcag catgtccgca atttcgttct gctgaatcag cgggttacgc cgtaaaattg
    13381 caaggatctc cttttcccgg ttattcatct tcaggctacc gctttttcag tttgttgcgc
    13441 tttcagccag gcgatctctt ctgcccagat atccgggttg atggtttcaa ggatcagcgg
    13501 aatgccgtcg aaacggtcgt tctgcataat ccagcggaac gcatcatgac cgatattgcc
    13561 ttcaccgagg ctatgatggc ggtcaacgcg gctgccaaag gtacttttcg catcgttaag
    13621 gtgcatcccg cgcaaatact taaagccgac aatacgggca aaatcagcga acgttttttc
    13681 gcattcggct ggagtacgca aatcataccc ggcagcgaaa gcatggcagg tatcaatgca
    13741 gacgccgacg cgggatttat cttccacgcc gtcgataatc gccgcgagat gttcgaattt
    13801 aaaccctaag ttactgccct gaccggcggt gttttcaatc accgctgtta caccttgagt
    13861 tttatccagc gcaatgttga tggattcggc aatacgcgca aggcaatcct cttctgaaat
    13921 ctgcatcaga tggctgccag ggtggaagtt gagcaaagaa agccccagtt gttcgcaacg
    13981 ctgcatttca tctataaagg catcgcgcga tttttccaga gcttcagtga ccggatgtcc
    14041 gaggttgatc agataactgt cgtggggaag aatttgcgcc gatgtgtagt gatatttttc
    14101 acaggcggct ttgaattcat cgatggtttg cgtcgtgagc ggtgcggcac gccactgacg
    14161 ttggtttttg gtgaacaagg caaacgcggt tgcgtcgatt tcggcggcgc gaattgcggc
    14221 atttgccaga ccgccagcag cactaacgtg cgctccaatg tatttcatgc gaggactcct
    14281 gttaaacccg ctggaggaaa acggtaatga tagcgggtta acaggagcaa gaagtagtgg
    14341 tttatgcgat gacgctttga atcacatagt taatcgcacc accaccaaca atcagccagg
    14401 caaacagtac cagtgccatc agcagaggtt tcgccccagc ttttttcagc gcgctgacgt
    14461 gagtggtcag acccagcgcc gccatcgcca ttgccagcag gaaggtatcc agcgttacca
    14521 gcatgttcac cacgctctgc ggtaacaggt ggaacgagtt aaagatggca actacgatga
    14581 acaagatggc aaaccacgga atagtgattt tgcttttctc gccgctgttc gccccagaca
    14641 gctgtttaac acgcgccgcc agcaggatga ggaacggagc cagcatcatc acgcgcagca
    14701 ttttggaaat aactgctgcg ttttctgcat ccgggctgat ggcatgacct gccgccacca
    14761 cctgcgccac ttcgtgcaca gtagaaccaa tgtagatacc gaaagtttcc ggactaaacc
    14821 attgagacat cagcggatat atcgccgggt agaggaaaat cgcgacggtc ccgaagataa
    14881 caacggttgc aacagccacg gttactttac tggcttccgc tttcactacc ggctcagtcg
    14941 ccagtaccgc ggcagcacca cagatactgc taccggcacc gatcaaccaa ctggtgtgct
    15001 tatccagacc aaacactttc tgccccagga agcaagccag caggaaggta ctggacagcg
    15061 tcagcacgtc aatgatgatc ccactgatac cgacatcggc aatttgcgag aacgtcagac
    15121 ggaagccata aagaatgata cccagacgta ataaatattg cttggcaaac agcacaccac
    15181 cgtcacagct tttccagatg tgcggataga tggtgttgcc taaaaccatc cccaacaaga
    15241 ttgcgagggt gagggcacta aacccggcac ccgcaaccgc gggaatggaa ccaccccaca
    15301 gggcgacccc ggtgataact gcactcaggg ctaaccccgg aataaaatgc cacagtgtac
    15361 gatgttgttt ctgtaaggtg atattcgtca taaccctctc ctttaaccgg gcataaggtt
    15421 acgactaact ggtttaaaaa taaaattgat tatatattta taattaatct ttataagtgg
    15481 taagcgacta tgcacatcac cctccggcag ttggaagttt ttgcagaagt attgaaaagt
    15541 ggatcaacca cccaggcgtc ggtgatgctg gcgttgtcgc aatcagcagt gagcgcagcc
    15601 ttgactgacc tggaagggca gcttggcgtt caactgtttg atcgcgtggg gaaaagactg
    15661 gttgttaatg aacacgggcg gctgctctat ccgcgagcgt tggcactgct tgaacaggcg
    15721 gttgaaatcg aacaactgtt tcgcgaagac aacggcgcga ttcgtatcta tgccagtagt
    15781 accatcggta actacattct gcctgcagtt atcgcccgtt atcgccatga ttatccgcag
    15841 ttgccgattg aacttagcgt tgggaacagc caggacgtga tgcaagcggt gctggatttc
    15901 cgcgttgata ttggctttat tgaaggaccg tgccacagca ctgaaatcat ttctgaaccg
    15961 tggctggaag acgagctggt ggttttcgcc gcgccgactt cgccgttggc tcgtggtccg
    16021 gtcaccttag aacagctggc cgctgcgccg tggatcctgc gtgaacgcgg ttccggcacg
    16081 cgggatattg tcgattatct gttgctgtca catttaccga agtttgagat ggcgatggaa
    16141 ttaggtaact ccgaggcaat caaacatgcg gtgcgtcatg ggttgggaat tagttgcctg
    16201 tcgcgacgtg tgattgaaga tcaattgcag gcaggcacat taagtgaagt tgcggtccct
    16261 ctgccgcgcc tgatgcgtac gttgtggcgt atacatcatc ggcaaaaaca cctttccaac
    16321 gcgctacggc gctttctgga ctattgcgat cccgcaaatg tgccgcgtta agttgctgta
    16381 caagaacatg ctggtgctgt gtcgatttcg tgacgcagcg ccttcagcat gcattcgcca
    16441 gaaaagagat tggctgcttt acttataatc cctgggcgat catgaaggtg tcttataacc
    16501 gtgtatttct gccggaagga ttgccaatcg tctgctacaa tcgcgccatc atttttaaga
    16561 tggatagcat ttttgtatgg gttccgaaac taaaactaca gaagcgccgg gcttacgccg
    16621 tgaattaaag gcgcgtcacc tgacgatgat tgccattggc ggttccatcg gtacaggtct
    16681 ttttgttgcc tctggcgcaa cgatttctca ggcaggtccg ggcggggcat tgctctcgta
    16741 tatgctgatt ggcctgatgg tttacttcct gatgaccagt ctcggtgaat tggctgcata
    16801 tatgccggtt tccggttcgt ttgccactta cggtcagaac tatgttgaag aaggctttgg
    16861 cttcgcgctg ggctggaact actggtacaa ctgggcggtg actattgccg ttgacctggt
    16921 tgcagcacag ctggtcatga gctggtggtt cccggataca ccgggctgga tctggagtgc
    16981 gttgttcctc ggcgttatct tcctgttgaa ctacatctct gttcgcggct ttggtgaagc
    17041 ggaatactgg ttctcactga tcaaagtcac gacagttatt gtctttatca tcgttggtgt
    17101 gctgatgatt atcggtatct tcaaaggcgc gcagcctgcg ggctggagca actggacaat
    17161 cggcgaagcg ccgtttgctg gtggttttgc ggcgatgatc ggcgtagcta tgattgtcgg
    17221 cttctctttc cagggaaccg agctgatcgg tattgctgca ggcgagtccg aagatccggc
    17281 gaaaaacatt ccacgcgcgg tacgtcaggt attctggcga atcctgttgt tctatgtgtt
    17341 cgcgatcctg attatcagcc tgattattcc gtataccgat ccgagcctgc tgcgtaacga
    17401 tgttaaagac atcagcgtca gtccgttcac cctggtgttc cagcacgcgg gtctgctctc
    17461 tgcggcggcg gtgatgaacg cagttattct gacggcggtg ctgtcagcag gtaactccgg
    17521 tatgtatgcg tctactcgta tgctgtacac cctggcgtgt gacggtaaag cgccgcgcat
    17581 tttcgctaaa ttgtcgcgtg gtggcgtgcc gcgtaatgcg ctgtatgcga cgacggtgat
    17641 tgccggtttg tgcttcctga cctccatgtt tggcaaccag acggtatacc tgtggctgct
    17701 gaacacctcc gggatgacgg gttttatcgc ctggctgggg attgccatta gccactatcg
    17761 cttccgtcgc ggttacgtat tgcagggaca cgacattaac gatctgccgt accgttcagg
    17821 tttcttccca ctggggccga tcttcgcatt cattctgtgt ctgattatca ctttgggcca
    17881 gaactacgaa gcgttcctga aagataccat tgactggggc ggcgtagcgg caacgtatat
    17941 tggtatcccg ctgttcctga ttatttggtt cggctacaag ctgattaaag gaactcactt
    18001 cgtacgctac agcgaaatga agttcccgca gaacgataag aaataagttt cctcccttcc
    18061 ttgctaagcc ctctcaaccg agagggcttt ttcaattcca tttccctgac aaatcatgcg
    18121 gatataaaat ttaacatttg gattgataat tgttatcgtt tgcattatcg ttacgacgca
    18181 atcaaaaaag gctgacaaat cagaggctgt tccggctttc tgggatgatc acctgcatac
    18241 aaataagtcc accgcgatgc tgccgtacgc aaggggacgt aaagaagatg tgagcgatac
    18301 ccattttatt ttcgtagtta cctcatggag atatggaatg tttaggttga accctttcgt
    18361 acgggtcggg ctgtgtttgt ccgctatttc ttgtgcatgg cctgtgttag cggtcgatga
    18421 tgatggcgaa acgatggttg tcactgcatc ttccgtggaa caaaatctta aagatgcacc
    18481 cgccagtatc agcgtcatta cccaggaaga cctgcagcga aaaccggtac agaatctgaa
    18541 ggatgtcctc aaagaagtgc ctggcgtaca actgacgaac gaaggggata accgtaaggg
    18601 cgttagtatt cgtggtctgg acagcagcta caccctgatt cttgtcgacg gtaaacgcgt
    18661 taactcccgc aatgccgtct tccgccacaa tgatttcgat ctgaactgga tcccggtcga
    18721 ttccatcgaa cgtattgaag tggttcgtgg cccgatgtcg tcgctatacg gctccgatgc
    18781 gctcggcggt gtagtgaata tcatcaccaa aaaaatcggt cagaaatggt cgggtaccgt
    18841 taccgtcgat accaccattc aggaacatcg cgatcgcggt gatacctata acggtcagtt
    18901 ctttaccagt ggaccattaa ttgatggtgt gctgggaatg aaagcttacg gcagcctggc
    18961 aaaacgtgaa aaggatgacc cgcaaaactc aacgaccacc gataccggag aaacgccgcg
    19021 tattgaagga ttctccagcc gcgacggcaa tgtcgaattt gcctagacac cgaatcaaaa
    19081 tcacgatttt actgccggat acggtttcga ccgtcaggat cgtgattccg attcgctgga
    19141 caaaaaccgc ctggaacgcc agaactactg cgtcagccat aatgggcgtt gggattacgg
    19201 caccagcgaa ctgaaatcct acggtgagaa agtcgagaac aaaaaccctg gcaacagcag
    19261 cccgataact tccgaaagca atacggtcga cggcaaatac acgttgccgc tgacggcgat
    19321 taatcagttt ctcacggttg gcggtgaatg gcgtcacgac aaacttagcg atgcggtgaa
    19381 cctgaccggg ggaaccagct ccaaaacgtc tgccagccag tacgcgctgt ttgtggaaga
    19441 tgaatggcgg atcttcgagc cgctggcgct gacgaccggc gtgcgtatgg acgatcacga
    19501 aacctacggt gaacactgga gtccgcgtgc ctacatggtt tataacgcca ccgacaccgt
    19561 aacggtgaaa gggggctggg cgacggcatt taaagcacct tctctgttgc aacttagccc
    19621 tgactggacg agcaattcct gccgtggcgc atgtaagatt gtgggtagcc cggatctgaa
    19681 accagaaacc agcgaaagtt gggagctggg gctttactac atgggtgaag aaggctggct
    19741 ggaagggggt gaatccagcg ttatcgtttt ccgtaacgat gtgaaagatc gtatcagcat
    19801 cagccgtacg tctgacgtca acgctgcacc gggctaccaa aactttgttg gttttgagac
    19861 gggcgctaac ggacggcgca taccggtatt tagctactac agcgttaaca aagctcgtat
    19921 tcagggcgtg gaaaccgaac tgaaaattcc gttcaacgat gaatggaaac tgtcgatcaa
    19981 ctacacctac aacgatggtc gtgatgtcag caacggcgaa aacaaaccgc tatccgatct
    20041 gccgttccat actgctaacg gtacgctgga ctggaaaccg ctggcgctgg aagactggtc
    20101 attctatgtt tctggtcact ataccgggca gaaacgcgcc gacagcgcga cggctaaaac
    20161 accgggcggt tataccatct ggaataccgg cgcggcctgg caggtgacta aagacgtcaa
    20221 actgcgcgca ggcgtgctga accttggcga caaggatctc agtcgtgacg actacagcta
    20281 taacgaagac ggacgtcgtt actttatggc agtggattat cgcttctgat gagaagatgc
    20341 ccggcgaacc gggcggactt tcacttcagt aaatactgcg catggaagcg caggtgatcc
    20401 tctataaaag aggcgatgaa gtagtaactg tgatcatatc ccggctgaat acgcagcgtc
    20461 atcggccacg ctttctggcg cgcggcttct gccaataccg caggttgcaa ctggtcggca
    20521 agaaactgat cattatcgcc ctgatcgata agcgtcggga tcgcatcctg cgcgttactg
    20581 gcatacatca gcgcgcaact atcccattcc agcatgcatt tttgtcctca cctaaatagg
    20641 tgctaaacgc tttgattccc cacgggacgc tgcacggatt cacaatgggc gcaaaggccg
    20701 aaacgctggt gtatttaccc ggatttttca gcgccataat cagcgcaccg tgaccaccca
    20761 ttgagtgacc gctaatggcg cagcggtcgc tgacattaaa ttgcgactga accagcgccg
    20821 gtaattcatc gcgcagataa tcatacatcc ggtaatgcgt cgcccacggc ggttgcgtgg
    20881 cattaagata aaagcctgcg ccctggccta aatcgtagcc atcgtcgttg gcaacctttt
    20941 cgccgcgcgg gctggtgtct ggcatcacca gtacaatccc cagttccgcc gctacccgct
    21001 gggcacccgc cttggtggtg aagttctcgt cattgcaggt taatccggaa agccagtaca
    21061 gcactggtgg cggggtgtga tcacgagggg gggggagaaa gatactgaac gtcatcgggc
    21121 agtttaaggt actggagtcg tgtcgccagc gttgctgcca gccttcaaaa cagcggtgct
    21181 cttcgagcat ttccatgcgt ggctccttgt tgtgttgttt gcaatttctt catcataccg
    21241 tttattcaat gtgctgtgag taactttcac ttccgtattt gcataacgat gttttaacat
    21301 ctgctgatga aaggcagcgg caattacaat aattatcgct gtgaatactg gattatgtgc
    21361 gccgcctcac gcacaataat caagctgtaa atcagcttaa taactttgcc cccacgcagg
    21421 gtggaggcgt cacacctgca ggagaaatca taaatgccat cactcagtaa agaagcggcc
    21481 ctggttcatg aagcgttagt tgcgagagga ctggaaacac cgctgcgccc gcccgtgcat
    21541 gaaatggata acgaaacgcg caaaagcctt attgctggtc atatgaccga aatcatgcag
    21601 ctgctgaatc tcgacctggc tgatgacagt ttgatggaaa cgccgcatcg catcgctaaa
    21661 atgtatgtcg atgaaatttt ctccggtctg gattacgcca atttcccgaa aatcaccctc
    21721 attgaaaaca aaatgaaggt cgatgaaatg gtcaccgtgc gcgatatcac tctgaccagt
    21781 acctgtgaac accattttgt taccatcgat ggcaaagcga cggtggctta tatcccgaaa
    21841 gattcggtga tcggtctgtc aaaaattaac cgtatcgtgc agttctttgc ccagcgtccg
    21901 caggtgcagg aacgtctgac gcagcaaatt cttattgcgc tacaaacgct gctgggcacc
    21961 aataacgtgg ctgtctcgat cgacgcggtg cattactgcg tgaaggcgcg tggcatccgc
    22021 gatgcaacca gtgccacgac aacgacctct cttggtggat tgttcaaatc cagtcagaat
    22081 acgcgccacg agtttctgcg cgctgtgcgc catcacaact gattaaaagg caggaaccat
    22141 ggagcgcaac gtcacgctcg attttgttcg cggcgtcgcc attctgggga tcctgctatt
    22201 aaacatcagc gcctttgggc taccaaaggc ggcttatctc aatcccgcct ggtacgatgc
    22261 tattacgccg caggatgcat ggacctgggc atttctcgat ctcatcggcc aggtgaaatt
    22321 cctcacgctt tttgcgctgc tgtttggtgc tggcctgcaa atgttgctgc cccgtggcag
    22381 acgctggatc cagtcgcggt taacgctgtt agtcttgttg ggctttattc acggtttatt
    22441 gttctgggac ggcgatattc tgctggctta cgggctggtg gggcttggcg ttttgctgtt
    22501 attggggctg atttccgaca gccagaccag ccgcgcctgg acgccggatg catcggctat
    22561 tttgtatgaa aaatactgga agcttcacgg cggcgttgaa gcgatcagta atcgtgccga
    22621 tggtgttggc aacagtttac tggcactggg cgcacagtat ggctggcaac tggcagggat
    22681 gatgctcatt ggtgcggcgc tgatgcgcag cggctggctg aaagggcagt tcagcttacg
    22741 tcactatcgt cgtactggtt ttgtgctggt ggcgattggg gtgaccatta accttcctgc
    22801 catcgccctg caatggcagc tggactgggc gtatcgctgg tgtgccttct tacttcaaat
    22861 gccgcgggaa ctgagtgcgc cgtttcaggc gattggctat gcgtcgctgt tttatggctt
    22921 ctggccgcaa ttgagccgct ttaagctggt gcttgcgatc gcctgcgtcg gacggatggc
    22981 gctgaccaac tatctattgc aaacgctgat ttgtaccacg cttttttacc acctcggttt
    23041 gtttatgcat tttgaccgcc tggagctgct ggcgtttgtt attccggtat ggctggcgaa
    23101 tatcctcttc tctgttatct ggctgcgtta cttccgccag gggccggtgg aatggctctg
    23161 gcgtcagtta actttgcgtg ctgccggacc ggcaatatct aaaacatcaa gataacgatc
    23221 tggattacat tcgttaacaa aacggctgta accgtttcca ttgctgtgac tcgattcacg
    23281 aagtcctgta ttcagtgctg acaaaatagc cgccagcaag cagtcattta ctgcaatctc
    23341 ataacaggta gtgaatatga tcaccattcg tgatgtagcg cgtcaggctg gcgtctctgt
    23401 ggcaacggtt tcccgggtgc tcaataacag cacgctggtc agtgccgaca cgcgtgaagc
    23461 agtaatgaaa gccgtgagtg agctggatta tcggccaaac gccaatgccc aggcgctggc
    23521 aactcaggtt agcgacacca ttggcgtggt ggtgatggac gtttctgatg cgtttttcgg
    23581 cgcgctggta aaagcggtgg atctggtcgc ccagcagcat cagaaatacg tgctaatcgg
    23641 caatagctat catgaagcgg aaaaagagcg tcacgccatt gaggtgttaa ttcgccagcg
    23701 ttgtaatgcg ttgattgttc actcaaaagc attgagtgac gatgaactgg cgcaatttat
    23761 ggataacatt cccggtatgg tgttaatcaa ccgcgttgtg ccggggtacg cccatcgttg
    23821 cgtttgcctg gataatctca gcggtgcccg aatggcgacg cgcatgttgc tgaataacgg
    23881 tcatcaacgt attggttatc tttcttccag ccacggcatt gaagatgacg ccatgcgtaa
    23941 agcaggctgg atgagtgcgt tgaaagagca ggatattatt ccgccggaaa gctggattgg
    24001 cactggtacg ccggacatgc cgggcggtga ggcggcgatg gttgaactgc tggggcgcaa
    24061 tctacaactt accgctgtat ttgcttataa cgacaatatg gccgctggcg cactgacagc
    24121 attaaaagat aatggcattg cgattccgtt acatctctca atcatcggtt tcgatgatat
    24181 tcccatcgcc cgttacaccg acccgcaatt aacgaccgtg cgttatccca ttgcttcaat
    24241 ggctaaatta gccaccgaac tggccttgca gggggcagca ggcaatattg atcctcgtgc
    24301 cagccactgt tttatgccga cgttagtgcg tcgccattct gtcgcaacgc gccagaatgc
    24361 ggcggcgatc actaactcaa caaatcaggc gatgtaaccg ctttcaatct gtgagtgatt
    24421 tcacagtatc ttaacaatgt gatagctatg attgcaccgt tttaatgttg taacccgtat
    24481 gtaacagtga ataatcactt tcaccgaggt aatagcgtca taacaacaat taaagccgtt
    24541 ttctggagcg ttaccgggca tggaagaacg aattttaaaa agtgagcttc ggcgttcagt
    24601 aacacttcat taactctact gccccgccga gcatttatct caagcactac cctgcataag
    24661 aaaaaccgga gataccatga ataagaaggt gttaaccctg tctgctgtga tggccagcat
    24721 gttattcggt gccgctgcac acgctgctga tactcgcatt ggtgtaacaa tctataagta
    24781 cgacgataac tttatgtctg tagtgcgcaa ggctattgag caagatgcga aagccgcgcc
    24841 agatgttcag ctgctgatga atgattctca gaatgaccag tccaagcaga acgatcagat
    24901 cgacgtattg ctggcgaaag gggtgaaggc actggcaatc aacctggttg acccggcagc
    24961 tgcgggtacg gtgattgaga aagcgcgtgg gcaaaacgtg ccggtggttt tcttcaacaa
    25021 agaaccgtct cgtaaggcgc tggatagcta cgacaaagcc tactacgttg gcactgactc
    25081 caaagagtcc ggcattattc agggcgattt gattgctaaa cactgggcgg cgaatcaggg
    25141 ttgggatctg aacaaagacg gtcagattca gttcgtactg ctgaaaggtg aaccgggcca
    25201 tccggatgca gaagcacgta ccacttacgt gattaaagaa ttgaacgata aaggcatcaa
    25261 aactgaacag ttacagttag ataccgcaat gtgggacacc gctcaggcga aagataagat
    25321 ggacgcctgg ctgtctggcc cgaacgccaa caaaatcgaa gtggttatcg ccaacaacga
    25381 tgcgatggca atgggcgcgg tagaagcact gaaagcacac aacaagtcca gcattccggt
    25441 gtttggcgtc gatgcgctgc cagaagcgct ggcgctggtg aaatccggtg cactggcggg
    25501 cacagtactg aacgatgcta acaaccaggc gaaagcgacc tttgatctgg cgaaaaacct
    25561 ggccgatggc aaaggtgcgg ctgatggcac caactggaaa atcgacaaca aagtggtccg
    25621 cgtaccttat gttggcgtag ataaagacaa cttggctgaa ttcagcaaga aataagactg
    25681 attgggcgca gtcgtttact gcgcccttat tgtatggcca acaaggtaaa attatatggt
    25741 cagctcaacg actccgtctt ccggggaata cttgttggaa atgagcggta tcaacaagtc
    25801 ctttcctggt gttaaggcac ttgataacgt taatttaaaa gtccggccac attctatcca
    25861 tgcattaatg ggggaaaacg gtgcaggaaa atcgacatta ttaaaatgcc tgtttggtat
    25921 ttatcaaaaa gactccggca ccattttatt ccagggtaaa gagatcgatt tccattccgc
    25981 caaagaggca ctggaaaatg gtatttcgat ggtacaccag gagttaaacc tggtattaca
    26041 acgttcggtg atggataaca tgtggctggg gcgatatccc accaaaggca tgtttgtcga
    26101 tcaggacaaa atgtaccgcg aaaccaaagc gatttttgat gaactggata ttgatatcga
    26161 tccgcgtgcg cgcgtcggca cattatccgt ttcgcaaatg cagatgatcg aaatcgccaa
    26221 agcgttttcc tataacgcga aaattgtgat tatggatgaa ccgacttctt cgttaaccga
    26281 aaaagaggtc aatcatctgt tcactattat tcgtaaatta aaagagcgcg gctgcggtat
    26341 tgtttatatc tcgcataaaa tggaagaaat cttccagtta tgtgatgaag ttaccgtatt
    26401 gcgcgacggt cagtggatcg ccaccgaacc gctggcagga ctgacgatgg acaagatcat
    26461 cgccatgatg gttgggcgtt ctcttaatca gcgttttcct gacaaagaaa acaagccggg
    26521 cgaagtcatc ctcgaggtgc gtaacctaac gtcactgcgc cagccgtcga ttcgcgatgt
    26581 ctcgtttgat ctgcataaag gggagatcct cggtattgcc gggctggtgg gggcgaaacg
    26641 taccgatatt gttgagacgt tatttggtat tcgcgagaaa tcggctggca ccattacgtt
    26701 gcacggcaaa cagatcaata accataatgc caacgaagcc ataaaccacg gatttgcact
    26761 ggtaactgag gagcgccgct caacgggaat ttatgcctat ctggatattg gttttaactc
    26821 gttaatttcc aatattcgca actacaaaaa taaagttggt ttactggata actcgcggat
    26881 gaaaagcgat acccagtggg tgattgattc gatgcgggta aaaacgccgg gtcatcggac
    26941 gcaaattggt tcgctctccg gtggtaatca gcaaaaggtg attattggtc gctggctact
    27001 aacgcaacca gaaatattaa tgctcgatga accgacgcgc ggtattgatg tcggggcgaa
    27061 gtttgaaatt tatcagttaa ttgccgaact ggcgaagaaa ggcaagggga ttattattat
    27121 ctcctctgaa atgcctgagt tgttagggat aacagaccgt attctggtca tgagcaatgg
    27181 tctcgtttcc ggaattgtcg atacaaaaac aacaacgcaa aacgaaattc tgcgtcttgc
    27241 gtctttgcac ctttaagatt aggggcttcc catgagtgcg ttaaataaga aaagttttct
    27301 tacttacctg aaagagggcg gtatttacgt cgttctttta gttttgctgg cgattattat
    27361 tttccaggac ccaacatttt taagtctgtt gaacttaagt aatattctca cccagtcatc
    27421 ggtgcgtatt attatcgcgc tcggtgtggc agggttaatt gtcacccagg ggaccgatct
    27481 ttctgctggt cgtcaggtag ggctggcggc agtggtggct gcgacattat tgcagtccat
    27541 ggataacgcc aacaaagtgt tcccggaaat ggcgacgatg ccgattgcgc tggttattct
    27601 gattgtctgt gccattggtg cggtgatcgg tttgatcaac ggtctgatta tcgcttatct
    27661 caacgtgacg ccgttcatta ccacgctcgg cacgatgatc atcgtctatg gcatcaactc
    27721 gctctattac gactttgtcg gggcgtcgcc aatttctggt tttgacagtg gcttctctac
    27781 ctttgctcag ggctttgtcg cgctggggag tttccgtctc tcttacatca ccttctacgc
    27841 gttgattgcg gtggcgttcg tctgggtgtt gtggaacaaa acccgcttcg gtaagaacat
    27901 ttttgccatt ggcggtaacc cggaagcggc aaaaatatct ggtgtcaacg tcggcctgaa
    27961 cctgctgatg atctacgcgt tgtctggcgt gttctatgcc tttggcggga tgttagaagc
    28021 cggacgtatc ggctctgcca ccaacaacct cggctttatg tatgagctgg atgctatcgc
    28081 ggcgtgcgtg gtaggcggtg tatcgttcag cggcggtgtg gggacggtga ttggcgtggt
    28141 gaccggggta attattttta ccgtcatcaa ctatggcctg acgtatatcg gcgtaaaccc
    28201 atactggcag tacatcatca aaggggcgat tattatcttc gccgtagcgc tggattcact
    28261 gaaatacgcg cgtaagaagt gatgtgattg cggatattgc cggatgcgtc tctggtttta
    28321 caacgttacc gggtgttctt tctcgccttt cttaaacttc acttccccga gctcaataca
    28381 acccaccggg cagacgtgac cacaaagcag acaacccaca catttctcgg tattacaatg
    28441 cggtgtgcgg gttttctcgc tccattccat cgcctggtga ccgccgtcgt aacaggaaat
    28501 ataacagcgt ccacagccaa cacatttgtc aagattgata cggggataga caatataact
    28561 gcggtctaaa tcttccgccg ggacaatatt gttattcgcc agacctacca tctcctgcaa
    28621 cgaatcaaat ccctgatcgg cgagataatg cgataacccg ctcgccatat cttctacgat
    28681 gcgatagccg tactgcataa tgccggtggt gacctgtaac gttgctgcgc cgagcaataa
    28741 aaactccgcc gcatcttccc aggtttcaat gccgccgata ccgctgattg ggaaatcgcg
    28801 cagttctgga tgggtgcgca tttgctgaat gaagcgcagg gcgatcggtt ttaccgcttt
    28861 accggaatat ccggaaatac tcgatttacc gttaacgatc ggcataccga ttttctgatt
    28921 aagatcgata ttggtgatgg atttaacggt gttaattgcc gcaatgccat ctgcgccgcc
    28981 gcgcttcgcc gccagcgcca cttcgcacat atcaccgata ttcggcgtca tcttcgccag
    29041 cattggcagc gtggaacccc gtttcactgc ccgacaatat ttttctacca gctccgggct
    29101 ttgcccgaca tcgctaccca tcgcatgaga agtcatttgc ggacaggaga agttacactc
    29161 gatcatatcc gcgccagctg ctcccattgc tgctcatttt cccccatgat cgaagcgatc
    29221 aatactttgt ccgggtaatc ttccttcagc cgacgcaggg cggtcagatt ctcttccaac
    29281 ggatgttcag caatctgctc catatttttg aagccgataa aaccggtatc ttctttcacc
    29341 agatgatcaa aacgcggcga gacttcgttg gcgataaaaa agccgatcgt tttaaacacc
    29401 acaccgcccc aacctgtgtc gtaggctttg gcacacatct catagcagtt gcctaccggc
    29461 gaagaagaga ggcagaacgg gttgggaaag ttcacgccgc aaaaagtaat cgaaagatct
    29521 ttcgttaaca tgagcaagct ccctctaaat agtgatgaat cgcctcggcg gcttctttcc
    29581 cggttttcac ggcatagacc acggttttgt caccctcaac aatatcgcca gcagcaaaga
    29641 cttgcggatc gcggatctga taattttgtg ttttgatggt gttacgctgc tgctctaact
    29701 ccgcaaaggc atccagtctg gcatgctgac cgacggcgag aataatttta tctgccgcca
    29761 tcgtcagttc gcctgataac cgtacatgct taaacgtgac tttattgcct tccacggcta
    29821 ctggcgtgaa tccatcgatg atcgaaacgc ccagctcccg ggcgctggca aactcttttt
    29881 cgcttgccgg aaactcatct aactcttcac gtgctacgca agttaccgcc tgacagccga
    29941 gaactttcag cgtgctggct acgtccatcg cgacatcacc gccgccgata attaatgcgc
    30001 tttgtggaat gctgatatcg ccttgcgcct gtcgtacacg ttgcaggaag tcgacggcaa
    30061 tctcaacgtc gctatgctca aacagcggta gcccggaacc gcttgataac cccacggtga
    30121 ccagtaccgc gcggttttcc gctttcagtt gctccagggt gagtgtttta ccgatttcgt
    30181 tattgcactt aatggtcacg cccatttttt caatacgggc gatctctgca tccagcactg
    30241 attgcggtaa ccggaattgc ggaataccgt tacgcaacca gccaccgggg tgcgcttctt
    30301 tctcataaat cgtgacgtca taaccctggt ttgtcagtgt cacactggcc tgcaatccgg
    30361 caggacctgc gccaataatc gcgactttgc cgagcgtttt agtaccgggc tgataaattt
    30421 ccatcccggt ttgttgttca aaatcagtaa caaaacgctg taagcggccg atatcaatgg
    30481 gagcatcaac accggcacgg gtacaaccgc tttggcataa tttctccgtc gggcacactc
    30541 tggcacaaac ggcaccgagg gcattatttt cgcgaattgt ctcggcagcg cctttaaaat
    30601 tacgaaagta gattgagcga ataaatttcc ccggatcggt ctgtgccggg caagcctgac
    30661 tacagggagc gtcgtgacat aataaacagc gagaggcttc tttaatcgcc agtaacggcg
    30721 taaaagccgg agtgagttca tccagataat tttgttgcgg catgtgatct tccttaatgc
    30781 tatgagtaat tatggcgaac gctaaataaa tattgcgtgc tgatattaag gctatgcgcg
    30841 tggggaaatt taagctgtga aaggattcac aaatagctgc ggttcagaat gttatatccg
    30901 cagcttattg agtgaagtaa taattattct caataattaa cagaaaagag aaaatattaa
    30961 cgatagaaaa taagataaac aatcatgccg atagataaaa ttccgaagcc aatatatgcc
    31021 accatataag cgaagaagag tccgggattt tttgctcgcc agacatacac cggttccggt
    31081 gcatttttaa cccgtttatc aatcgcgcct gcgcgccagc ctttccatgc ttcgcggaaa
    31141 caaaatatcg ggataaggaa aaaaacggca acgacaaaaa actgctgtac atccataact
    31201 actttccttg tttcttttgt aattcaagta actgttcggg tgtaacggcg ggatacccct
    31261 gcgcatcttc cggcacctgg tgcatagccg gaatagggac cagcggccct aaaaaacgcg
    31321 gttcacgttt aaaaatataa aggtcagcca gcgcaccgaa acgggcggca aattcgcgaa
    31381 tacgtactga cagcatatct ttcggtgacg gtacggcata acactgagcc tgaatcccca
    31441 tatgcagcgc aataaataat gctcgctcac agtggaaacg ttgggtgata ataatgaaat
    31501 cattagtatc gaaaactttg cgtgtacgca cgatggaatc cagcgtacga aagcctgcgt
    31561 aatcgagaac aatatctgat gggtcgacac cagcagcgat taaatctttg cgcatggtca
    31621 tcggctcgtt ataactttgc aatgcgttat cgccgctcag taatagataa tttaccttac
    31681 cgctgttata ggcattaatc gctccttgaa tgcggtagcg ataatactga ttaataacgc
    31741 cggtacgata atattttgct gtgccgagca ccacgccgac ctggcggtag gggagatcct
    31801 gcaattcgtc gtagatataa ggcgcggttt tccagctcat ccagcgatcg aggccgagca
    31861 cagtcaacag cagcaagccg atcaggacta acaggctgag gaacacgcgc tttaacatgc
    31921 aggagactcg agctaaggaa aatttccatc aggctacttt acccgctggt tttgcgcaag
    31981 aaacggcaaa ctgtttgccg ggaatttcga caccaggaga gggctggcaa gccggacaag
    32041 gcgttcacgc cgcatccggc accaggctta agcgagaagc acgcggtcga tactgtgaca
    32101 gccgagagct ttcagcgttg cggaggtggc atcccactga atcaacggcg catcggcttt
    32161 ttctgccaga accgcgcgct ggatatccgg gtaatcataa cccttgagat tcaacagaat
    32221 taacgccccc tgcaacggcg gcagagtcgg gttgaatgcg gcgttttcag cgtagctgcc
    32281 gctgaaaata cgaccgtctt tacattccag cgcgacaccg cttggtgact tactgtaagg
    32341 catgtgcgaa cggtttgccg ctgcaatcgc cgcctgagaa agcgcatcac cagtcagcgc
    32401 atagccgtga tcctgctcgt ccatcagcag cgttttaatc tccagatctt tcggcccaaa
    32461 ggcatccggc agatagtcac gcagcgcgtg tgcctcgcgg cccggcaaat gaatacgcag
    32521 atccagaccg ctgttcagtt cattcataaa ctgacggcag tgaccacaag gcgtatagtt
    32581 aacggtgatg gctgcaagcg ctttttcacc actcaaccaa gcgtggctga tcgcgctttg
    32641 ttcggcatga acggtttgct gcattgttgc accaataaat tccatattgg caccaaaata
    32701 ccaggttccg ctcacaccgc gcgcaattgc gccaacatta aaattcgaca atggcgtacg
    32761 cgcacaggcc gccgccagcg gaagtagtgc gaatgccagc gcgtcttcgt ccagtcccgt
    32821 tgcgctcttc agcgatgaga cttgctcccc ggtcaacaaa gcggggaagt acttgtctgc
    32881 cagaataggt tccagtgcag attgcaagtt atccgcaagt tgggcaaaag cggtttgaaa
    32941 acgtggatgc atggcgttgc ctcataatca tgtaatggga taacagttta cgaacccgtt
    33001 atggctttat atgtgatctg aatctcatta attatgcaac acgtgaaact tacatcaaaa
    33061 atgcgcgacg tatcgcaaat tttagcccat tactgccaga ataatcggga aaaggaacgg
    33121 tgcgatcagc gaagtaatta tcccgcataa caccagcgct agcgaactaa atgcaccttc
    33181 ctgataatcc agctcggcgc agcgcgccgt accgagggcg tgcgaggcag tccccattgc
    33241 cagaccgcgc gcagctttgg tacgaatacg catcgcatta agcaatgtat ggccaaatac
    33301 cgcgccgagg atgccgacga aaatcacgca aactgcgctg attgccggaa taccgccaat
    33361 actgccgcca accgccattg caataggcgt ggtgactgat ttcggcagga ttgacgcggc
    33421 gatttccggt gaagcgccca tcaacaatgc cacggaagtc cccgtcacca tcgcaaccac
    33481 gctgccgata aaacagatgg tgatgatcga tttccagcgc gcgcggatct ggtgtagctg
    33541 ctcatataaa ggataggcca gcgcgacgac cgccggttgc agcagatcgt ttagcacttc
    33601 gctaccttta aagtaactgt cgtaagagat gccggtcagc attaaaaaag gaatgatcac
    33661 caccattgct accagcaacg ggttaagcag gggaaactta taccgtgccg ccagtttgcg
    33721 ggcggcaaaa aagacaatca acgtcagcgg taatgaccac cagatattcg ccatcattct
    33781 tctgatcctt tctgacctac gactttacgt tcaccgtgta ccagttgtga actccagctc
    33841 accaccagaa aaaccaccag cgtactgaca gcacaggaaa ccactaccgg gccaaactgc
    33901 gcgcggagca aatcaaaata ttgcatgacg cctacgccaa tcggcacaaa caatagcgcc
    33961 atatagcgaa tcagtacgta gcaccccgga ttgacccatt tcgccggaag aatttgcaag
    34021 gccagcagga caaacaggat cagcatcccg atgatgctgc ccggaatggt taccggtagc
    34081 agggaagcaa tgaaaatgcc tgcatacagg caggcataaa tcaggacgaa agcgcgtaaa
    34141 tattgccaga taatgttcag tgtcttactc ataatcaaca ccccagatga gatggattca
    34201 tcatacaatt aattcgtaaa acgtgctacc gatcacatta agaaatcagc gcttattttt
    34261 tcgctacccg gtattacgca atgagcagcg aactcaggca tacatcaagc atagagaggg
    34321 attgcagttt gcagcaatcc ctctttgcag acctttaatc cgctgaccat aggcgctaac
    34381 atagcgcctc atttttttgc gggtgatgat atgcgtgtgt tactggcacc gatggaggga
    34441 gtgcttgact ctctggtgcg tgaattgctg accgaagtta acgactacga tctgtgcatc
    34501 accgagtttg tccgcgtggt ggatcaactg ctgccggtaa aagtctttca tcgcatttgc
    34561 cctgagctac aaaacgccag ccggacacca tctggtacgc tggtgcgcgt gcagttgtta
    34621 ggtcagttcc cacaatggct ggcagagaac gccgcccgtg cggtggagtt aggtttctgg
    34681 ggcgtggatc tcaattgcgg ctgcccgtcg aaaacggtta acggtagcgg cggcggggcg
    34741 acgttactca aagatcctga actcatctac cagggggcaa aagcgatgcg tgaagctgta
    34801 ccggcgcatt tgcctgtcag cgtgaaagtg cgtctgggct gggacagcgg tgagaagaaa
    34861 tttgaaatcg ccgatgcggt tcaacaggct ggcgctacgg agctggtggt gcatgggcgg
    34921 acgaaagagc agggttaccg cgcggagcat attgactggc aggcgattgg cgagattcgc
    34981 cagcggctga atattccggt gattgccaac ggtgaaatct gggactggca gagcgcgcaa
    35041 caatgcatgg cgatcagcgg ctgcgacgca gtgatgattg gtcgcggggc gctcaatatt
    35101 cccaacctga gccgggtggt aaaatataac gaaccgcgaa tgccgtggcc ggaggtggtt
    35161 gctttgctgc aaaaatatac ccgtctggaa aagcagggcg ataccgggtt atatcacgtt
    35221 gcgcggatta aacagtggtt gagttatttg cgtaaagaat acgatgaagc aacggaatta
    35281 tttcagcatg ttcgggtgtt gaataactcc cctgatattg caagggctat tcaggcaatt
    35341 gatatcgaga aactctaaaa accatagtga tgtttttgac aatgtgttag ctctttatca
    35401 ataggccgca cggttgtttg gtggcgtcaa aatcgccgag gcgttccctg aaggccgggg
    35461 cagcccacat ggatgtgggc tgagggcgcg ttttacaggg atgttacctc gcgcccgacc
    35521 cggtagccgt aagggataag ccgagggcac cgcgcagcgg cgattttgtt cgccagagcc
    35581 cgggggggca gggggcggcg gcgattggtc gccccctgcg cgctccttgc gccagtgatt
    35641 atatgaagca atgcacatga caggagcgca acaggatgag agactgaaat attctcgtta
    35701 ttttcggatc tgatgacatt aattaatttt aaaatatcat cttaaatacg ccagtcacca
    35761 ccagtagccc aatcaggaaa ataatcaata cagcccagag tataattttc attggcgttc
    35821 ctttatttgt gagtgctgaa taagtatagg aaaaaacgaa gccctttgcc gttatccata
    35881 aacgattaag ataaatcgga agatatcatt tacttagtct atatttttct gggtatgtcg
    35941 ttttattgct actgatgtat tgccacgcga aactaaaaaa tatatcgcaa cacacggtaa
    36001 tctccgcgca ccgtttctta cctgattatt cccccatgaa tcgtgattcc ttttatccag
    36061 ccatcgcctg ttttccgctg ttactgatgc tggccgggtg tgcgcctatg catgaaaccc
    36121 gccaggcgtt aagccagcaa acgcccgctg cacaagttga caccgcatta cccacggcgc
    36181 tgaaaaatgg ttggccagac agccaatggt ggctggagta tcacgataat cagctcactt
    36241 cattaattaa caatgcacta cagaacgcac cggatatgca ggtggctgaa cagcgcattt
    36301 agctagctga agcccaggcg aaggcagttg ccactcagga tggtccgcag atcgactttt
    36361 cggcggatat ggagcggcaa aaaatgtcgg cagaagggct aatggggccg tttgctctga
    36421 acgatccggc cgcaggtacg accggtccgt ggtacaccaa cggaactttt ggcttaacgg
    36481 cgggctggca tctcgatatc tggggaaaga atcgggcgga ggttactgcc cgcctgggta
    36541 cggttaaagc acgggcggcg gaacgcgagc aaacccgcca attgctggct ggcagcgtag
    36601 cccgcctgta ctgggagtgg caaacccagg cggcgttaaa cacggtcttg cagcaaatag
    36661 aaaaagagca gaacaccatt atcgcgaccg atcgccagct atatcagaac gggattactt
    36721 cttcagttga cggtgtggaa accgatatta atgccagcaa aacccggcag cagctcaacg
    36781 atgtcgcggg gaaaatgaaa attattgagg cacggttaaa tgcactcaca aatcaccaga
    36841 ctaagtcatt gaagcttaaa ccggtcgcgt tgccgaaagt ggcaagccag cttcctgatg
    36901 aactggggta ctccttactg gcccggcggg cagatttgca ggcggcgcac tggtacgttg
    36961 agtcatcgtt aagcactatt gatgcggcaa aagcggcatt ttatcctgac atcaacctga
    37021 tggccttcct gcaacaggat gcgttgcact taagcgatct gttccgtcat tccgcgcagc
    37081 aaatgggcgt tacggcaggc ctgacgctac ctattttcga tagtggtcgt cttaacgcca
    37141 atctcgatat cgcaaaagcc gaaagcaact tgtctatcgc cagctacaac aaagcggtgg
    37201 ttgaagcagt gaatgacgtg gcgcgggcag ccagtcaggt tcagacactg gcggagaaaa
    37261 accagcatca ggcgcaaatt gagcgcgatg ccttgcgtgt ggtaggtctt gcgcaggccc
    37321 gctttaacgc gggcatcatt gctggttccc gcgtcagcga agccagaatc ccggcgctgc
    37381 gtgagcgggc caatggcctg ttattgcaag ggcagtggct ggatgcctcc attcgactca
    37441 ccggtgcgct gggcgggggt tacaaacgct gatgatatgc gccttctata cttaacgttt
    37501 attcagcgtt aagtggagaa ctcgatggca taggtcgcga taattaccgc ttccgattcg
    37561 gggatcggca aagagtgcgc gttattactg gcgcagcagg ggtttgatat tggcattacc
    37621 tggcactcag atgaagaagg ggcaaaagat accgcgcgtg aggtggttac ccacggcgta
    37681 cgtgcggaga tcgtgcaact ggatctcggc aatctgccag aaggtgcgca ggcgctggag
    37741 acactcatcc agcgatttgg gcgtattgat gtgctggtga ataatgcggg tgcaatgacc
    37801 aaagcgccgt ttcttgatat ggcttttgat gagtggcgca agatttttac cgttgatgtt
    37861 gatggtgcat ttttatgctc gcaaattgcg gctcgtcaga tggtgaaata agggcagggc
    37921 ggtcgcatca tcaacattac gtcggtacat gaacatacgc cgctgccgga tgccagcgcc
    37981 tacacagccg ctaaacatgc gctcggcggg ttaaccaaaa cgatggcgct ggagttggtc
    38041 aggcataaga ttttggtgaa cgcagtcgcg cctggggcga tagccacgcc aatgaacagc
    38101 atggatgata gcgacgtgaa gcccgacgcg gagccttcga ttcctctacg gcgttttggc
    38161 gcaacgcatg agattgccag cctggtggtg tggctttgtt cggagggcgc aaattacacg
    38221 acagggcagt cgttgatagt ggatggcggc tttatgttgg cgaatccgca gttcaaccca
    38281 gaatagcgca gtgaggtgcg tggatatttc aatgggttag gccagataag acaccagcgt
    38341 cgcatcaggc atggaacacc cggtgccgga tgtggagtga atgcttatct gtggcaactg
    38401 ctttacgcct gattatccgg ctttttcttc ccgcgccgtt tcagccacca gcgcacgccc
    38461 accaccagaa ccacgaccag aatcaaccag acccagtgct tcaaatgctg gtcgagattg
    38521 tgcaaccacg gcgcaatcac ctgaccacca gcgtaaccaa tagtggtaaa aatcaacgcc
    38581 caggcaaatg cgccgagaat attcagcggc agaaagattt tcggcggcag ctggctggca
    38641 ccaatcagcg tcgggccaat cacccgaaag ccatacataa agcgcgtacc aatgacaaac
    38701 agatacggat ggcgttggat aagtttctgt gcccgctcaa ttttatcctg atgtttcgag
    38761 aaacggcgta acagcttgcc gccaaaccgc cgcccgcaca gatagagcac ctggtcgcca
    38821 atcatgccac caagcgccac agaaagtacc accagtggga actttaatag cccctgatgt
    38881 gccgcaacgc ctcccagcaa agtcacggtt tcaccttccg ccaggctacc gataaccagc
    38941 gcggcataac catattgtga gatgagtgta ttgagatcca taggccagtt tgtctccctt
    39001 tggttgcctg taagcataca ccctgtgaat cattcacgct gaaaaaggac atacatcaga
    39061 cggtgcaaat tacatggatg aacaaaaaat aatcacaagc tggcttatac ttgaactgtc
    39121 aggttcatga actcacaaca agggggcggt atgagccatg tctggggact gttttcccat
    39181 cccgatcgtg aaatgcaggt gattaatcgc gaaaacgaaa cgatttctca ccattacacc
    39241 caccacgttt tgctgatggc ggcgattccg gtgatttgcg ccttcattgg cactacacag
    39301 attggctgga attttggcga tggcactatc ctgaagttat cctggtttac cggactggcg
    39361 ctggctgtct tgttttacgg cgttatgctg gctggggttg cggtgatggg gcgggtcatc
    39421 tggtggatgg cgcgcaatta cccgcagcgt ccgtctcttg cgcactgcat ggtcttcgcg
    39481 ggctacgttg caactccgct gtttttaagc ggtctggtgg cgctttatcc actggtctgg
    39541 ttgtgtgcgc tggtcggcac ggtggcgctg ttttacaccg gctacctgct ttaccttggt
    39601 attccgagct tcctgaatat caacaaagag gaaggcctta gcttctccag ttcgacgctg
    39661 gcgattggcg tgctggtact ggaagtgctg ctcaccctaa ccgttatact atggggctac
    39721 ggttatcgtt tgttctagaa agttctttga cgttgcattg ctggcgtaaa tgtcagcaat
    39781 gcagcattta ctcacgattc tcctttgacg atcgcacttt ttgctcgtta tgatggcgtc
    39841 gctagcctca gtaaatcctt actccggcgg tgcgcaaccc gtgcgcgtga accactatct
    39901 gaatgctcat catgccgaaa tttcgagttt ctttatttag cttggccctg atgctggctg
    39961 tgccttttgc accgcaggcc gttgcgaaaa cggcagccgc taccaccgct tcacaaccgg
    40021 aaattgcctc cggtagcgcg atgattgttg atctgaatac caacaaagtg atctattcga
    40081 accacccgga tctggtgcgt ccgattgcgt ctatcagcaa attaatgacc gcgatggttg
    40141 tgctggatgc acgactgccg ctggatgaaa aactaaaagt ggatatcagc cagacgccgg
    40201 agatgaaagg ggtctattcg cgcgtacgac tgaatagcga aatcagccgt aaagatatgc
    40261 tgttgctggc gctgatgtct tcagaaaacc gcgcggcggc aagccttgcg caccattatc
    40321 ccggtggtta caaagccttt attaaggcaa tgaatgcgaa agcgaaatcg ctcggaatga
    40381 acaacacgcg ctttgttgaa cctaccggat tgtcggtgca taacgtttca actgcccgtg
    40441 acttaaccaa actgctcatt gccagcaaac aatatccgtt gatcgggcag ttaagtacta
    40501 cccgggaaga tatggcaacc ttctctaacc cgacgtatac gctgccgttc cgcaatacta
    40561 atcatctggt gtatcgcgat aactggaata ttcagttaac caaaaccggc tttaccaatg
    40621 cggcgggcca ttgtctggtg atgcgtacgg ttatcaataa taaaccggtg gcgctggtag
    40681 tgatggacgc gtttggcaaa tatacccatt ttgccgatgc cagccgcctg cgtacctgga
    40741 ttgaaaccgg taaagtgatg cctgtgccgg cagcagcgtt gagctataaa aaacaaaaag
    40801 ccgcccaaat ggcggcggcg gggcagacgg cacagaacga ttaagtgaat tcggatggcg
    40861 atactctgcc atccgtaatt tttactccac ttcctgccag tttttccgtt tactggtttt
    40921 accgatcccc ggattcatgc tattggtcgg atcattctcg cgatagaact tctgcaacgt
    40981 ctccggtgct ttatacaaat gaccgacgtt atgctcggca gggtactgcg cgccgcgctg
    41041 ctgtagcagt tccagcatct gttcttttaa cgcatgcaca tccacgcctt ttttcactat
    41101 gtaatcctga tggaagacat agcacataaa atggccgtaa tagagcttgt gcaccagctg
    41161 gctgtcgatc tccggcggta aatgctcata ccactcggtg tcgttacgcc gcagagcgat
    41221 atccagtgcc agaatgtctt cgacttcatc ggaatgcacc gcctgataac gaattgctgc
    41281 gcccgcagcg gcgaaacggt gtaaaaacgc tttgctacct tcctccggcg tacagacaaa
    41341 gaaatcgccc tcggcctgtt tgaaataatc caccagccac gatttggctt caccgacgcc
    41401 atcgcctgcc atttttaaca gcagatgatg ctcgtattta tcgcgccagt ttttcatgcg
    41461 cggcggtaaa tggctgggga acaggtgacc gaatttttgc attgcacggt cagtaaaatg
    41521 cggacggaag aatttcactt tctccagcat cgcatcggtg cgtcccttga gattaaagaa
    41581 gaacggcatc ttgtcggtgc ctaacttatc aatcatcagg aaggtgtctt tgccgtattt
    41641 ttccgcaata tcgtagatat cccggtgcat atattccccg gcaaccggca gattttcgaa
    41701 gttagccaga atatgacggc ggatttcggt cagcacttcc ggctggttgg tgccgatata
    41761 aaacacctgc tgattttttt ccgcttcgaa ggtatcaaga cgtaccgcaa agacggccag
    41821 cttcccggcg caaccagaag attcaaataa ccgatccgga tcggcgttat aacgtgcggg
    41881 cgtgtcggct tcaatatcac gaacgcggtg gacatagtca taatcgtggg cgtgacgacc
    41941 atcgtgacgc acatcgtcat ctttgatgcg atcgtcatcc agcttgctaa ggatctgctc
    42001 cggcgtttcg cccagatcaa tccccagatg gttcaccagc gtcagtttgc catcttcatc
    42061 tatacgcgcg aataacgaca tttcggtata cgccgggcca cgttgcacca gcgagccgcc
    42121 ggagttgtta caaataccgc cgatgaccga tgcgcctata cacgatgatc caatcaccga
    42181 atgcggttcg cgtcccagcg gtttgagggc tttttccagc gaatagagcg tggtgcccgg
    42241 ataggccagc acctgttcgc ccttgccgag aacgtgcagc ttgtcgagac gcagggtgct
    42301 gatgatcacg atatcgcgat catagtcgtt accgtttggc gtcgatcctt cggtcaggcc
    42361 tgtattggca gcctgcatca gaataatttt gtcggcggtg acgcaggctt tcagcacccg
    42421 ccacaattcc agtagtgagc cagggaaaac gacagccagc gcgtcgccct gaccagaacg
    42481 gaagcccttg cgatagcggg ccgtttttgc gggatcggtg agcaggtgtg aatgaccgac
    42541 cagacgggca agttcattca aaaaggcttt attatcagtt gttgtcatgg aagacatttt
    42601 ccactccttg tggtggcgaa aaaatatcac aaactagaat agcgccttgt atgacaattc
    42661 agcgagtgaa tattttgaaa attcagacaa taaactgagc attctttttc tctctccatc
    42721 atgcttatgg caaactacgg tttttgcacc attttcgctg ccacgtcggg caacaaagga
    42781 agaaaaatcc atatgaaatg gctatgttca gtaggaatcg cggtgagtct ggtcctgcag
    42841 ccagcactgg cggatgattt attcggtaac catccattaa cgcctgaagc gcgggatgcg
    42901 ttcgtcaccg atctgctcaa gaaaatgacg gttgatgaga aaattggtca gctgcgctta
    42961 atcagcgtag gcccggataa tccgaaagag gcgatccgcg agatgatcaa agacggtcag
    43021 gttggggcga ttttcaacac cgtaacccgt caggatatcc gcgccatgca ggatcaggtg
    43081 atggaattaa gccgcctgaa aattcctctt ttctttgctt acgacgtgct gcacggtcag
    43141 cgcacggtgt tcccgattag cctcggtctg gcctcgtctt ttaacctcga tgcggtgaaa
    43201 acggtcgggc gtgtctctgc ttatgaagcg gcagatgatg gcctgaatat gacctgggca
    43261 ccgatggtcg atgtctcgcg cgatccgcgc tggggacgtg cttccgaagg ttttggcgaa
    43321 gatacgtatc tcacctcaat aatgggtaaa accatggtgg aagcgatgca gggtaaaagc
    43381 ccggcagatc gttactcggt gatgaccagc gtcaaacact ttgccgcata cggcgcggta
    43441 gaaggcggta aagagtacaa caccgtcgat atgagtccgc agcgcctgtt taatgattat
    43501 atgccgccgt acaaagcggg gctggacgca ggcagcggcg cggtgatggt ggcgctgaac
    43561 tcgctgaacg gcacgccagc cacctccgat tcctggctgc tgaaagatgt tctgcgcgac
    43621 cagtggggct ttaaaggcat caccgtttcc gatcacggtg caatcaaaga gctgattaaa
    43681 catggcacgg cggcagaccc ggaagatgcg gtgcgcgtgg cgctgaaatc cggaatcaac
    43741 atgagtatga gcgacgagta ctactcgaag tatctgcctg ggttgattaa atccggcaaa
    43801 gtgaccatgg cagagctgga cgatgctgcc cgccatgtac tgaacgttaa atatgatatg
    43861 gggttgttta acgacccata cagccatttg gggccgaaag agtctgaccc ggtggatacc
    43921 aatgccgaaa gccgcctgca ccgtaaagaa gcgcgtgaag tggcgcgcga aagcctggtg
    43981 ttgctgaaaa accgtctcga aacgttaccg ctgaaaaaat cggccaccat tgcggtggtt
    44041 gggccactgg cggacagtaa acgtgacgtg atgggcagct ggtccgcagc cggtgttgcc
    44101 gatcaatccg tgaccgtact gaccgggatt aaaaattccg tcggtgaaaa cggtaaagtg
    44161 ctgtatgcca aaggggcgaa cgttaccagt gacaaaggca ttatcgattt cctgaatcag
    44221 tatgaagaag cggtcaaagt cgatccgcgt tcgccgcaag agatgattga tgaagcggtg
    44281 cagacggcga aacaatctga tgtggtggtg gctgtagtcg gtgaagcaca ggggatggcg
    44341 cacgaagcct ccagccggac cgatatcact attccgcaag gccaacgtga cttgattgcg
    44401 gcgctgaaag ccaccggtaa accgctggtg ctggtgctga tgaacgggcg tccgctggcg
    44461 ctggtgaaag aagatcagca ggctgatgcg attctggaaa cctggtttgc ggggactgaa
    44521 ggcggtaatg caattgccga tgtattgttt ggcgattaca acccgtccgg caagctgcca
    44581 atttccttcc cgcgttctgt cgggcagatc ccggtgtact acagccatct gaacaccggt
    44641 cgtccgtata atgccgacaa gccgaacaaa tatacttcgc gttattttga tgaagctaac
    44701 ggagcgcttt atccgttcgg ctatggtctg agctatacca ctttcaccgt ctctgatgtg
    44761 aaactttctg cgccgaccat gaagcgtgac ggcaaagtga cggccagcgt gcaggtgacg
    44821 aacaccggta agcgcgaggg ggccacggta gtgcagatgt acttgcagga tgtgacggct
    44881 tccatgagtc gccctgtgaa acagctgaaa ggctttgaga aaatcaccct gaaaccgggc
    44941 gaaactcaga ctgtcagctt cccgatcgat attgaggcgc tgaagttctg gaatcaacag
    45001 atgaaatatg acgccgagcc tggcaagttc aatgtattta tcggcactga ttccgcacgc
    45061 gttaagaaag gcgagtttga gttgctgtaa tgctttagta agggcgcgac gtttgcgccc
    45121 tttgcctgat gttcatatct taacttgctg aattttcgcc ctttgtaggc cggataaggc
    45181 gctcgcgccg catccggcag gaaacgccgg accgaatatg cctcttaagt atttatctga
    45241 ttctgcaact gaatccttcc gctcaggcta actctgtcat tatcaactat gcttttctct
    45301 taattcgctg aaaacaacag taaaagaatg aggaaagcat catgccactc ttaaagctct
    45361 gggcaggttc actggttatg ttggcagccg tgagcctgcc gctacaggcg gcttcccccg
    45421 ttaaagtcgg ttcaaaaatc gataccgaag gcgcgctgct cggcaacatc atcttgcagg
    45481 tactcgaaag ccacggcgta ccgacggtga ataaagtgca actcggaacg actcctgtgg
    45541 ggcgcggggc gattacttcc gatgaactgg atatctatcc ggaatatacc ggcaatggcg
    45601 ctttcttctt taaagatgaa aacgatgcag cgtggaaaaa tgccgggcag ggctacgaga
    45661 aagtcaaaaa gctcgatgcg gagcacaaca agttaatctg gctgacgccc gcgcctgcaa
    45721 ataacacctg gactatcgcc gtgcgtcagg atgtggcaga gaaaaacaaa ctcacttcgc
    45781 ttgccgacct gagtcgttat ctgcaagagg gcggcacctt caaactggca gcctcggcag
    45841 agtttatcga acgcgccgat gcgttaccca cgtttgaaaa agcctatggc tttaagctcg
    45901 gtcaggatca gttgctgtca ctggctggtg gcgacacggc ggtgacgatt aaagccgctg
    45961 cccagcaaac ctctggcgtt aatgccgcaa tggcttacgg cactgacggt ccggtcgcgg
    46021 cgctggggct gcaaacctta agcgatccgc aaggcgtgca acctatctac gcgcctgcac
    46081 cagtggtgcg tgaatcggtg ttgaaggagt atccgcaaat ggcacagtgg ctacagccag
    46141 tcttcgccag cctcgatgca aaaacattgc agcaactgaa tgccagcatt gcagtggaag
    46201 gactggatgc caaaaaagtg gctgccgact acctgaaaca aaaagggtgg gcgaagtaat
    46261 ttcccgtgac ttatctacgt attaatcctg ttctggcgct gctgttgttg ctgacggcaa
    46321 tcgcagcggc gctgccgttt atcagttacg cgcctaatcg tttagtctcg ggtgaagggc
    46381 ttcatctctg gcaactgtgg ccgcaaacga tctggatgct ggtgggcgtt ggttgcgcct
    46441 ggctgacagc ctgttttgtc cccgctaaaa aaggcagcat ttttgcactc attctggcgc
    46501 aattcgtctt cgtattgttg gtgtggggag ctggaaaggc ggcgacccaa ctggcgcaaa
    46561 atggcagtgc gctggcgcgt accagcctcg gcagaggttt ctggctggct gcggcgctgg
    46621 cattgctggc ctgtagcgat gccatccgcc gaatctccac gcatccgctg tggcgctggt
    46681 tgttgcatat gcagattgcc attattccgc tgtggttgct gtactccggc acgcttaacg
    46741 atctctcact aatgaaagaa tacgccaacc gtcaggatgt gtttgacgac gcgctggcac
    46801 aacatctgac gttgctgttt ggtgcggtgc tgcctgcgtt agtgattggt gtgccgttgg
    46861 gcatctggtg ctacttttcc actgcgcggc agggggcaat tttttctctg ctcaatgtca
    46921 ttcagaccgt gccttcggtg gcgctctttg gcctgttgat tgcgccgctt gccgcgctgg
    46981 ttacggcctt tccgtggctg gggaagctcg gcatagcagg gaccggaatg acacccgcac
    47041 tgattgcgct ggtgctctat gccttgctgc cgctggtgcg aggcgtggta gtcggtttga
    47101 accagatccc gcgcgatgtg ctggagagcg ccagagcgat ggggatgagc ggggcgcagc
    47161 gtttcctgca tgttcagtta ccgctggcgt taccggtatt tctgcgcagc ctgcgggtgg
    47221 tgatggtgca aactgtaggt atggcggtga ttgcggcgtt aatcggcgca ggcggttttg
    47281 gtgcgctggt tttccagggg ctgctaagca gcgccattga tttagtgttg ctgggggtga
    47341 tcccggtaat tgttctggcg gtgcttaccg acgcgctgtt cgatttgctt atcgcactgc
    47401 tgaaggtgaa acgtaatgat tgaatttagc catgtcagca aactgttcgg cgcacaaaaa
    47461 gccgttaacg atctcaatct caattttcag gaagggagtt tttcggtgct gattggcaca
    47521 tctggctccg gcaaatccac caccctgaaa atgattaacc gcctggtgga gcatgacagc
    47581 ggagtgatcc gctttgccgg agaagaaatt cgctcgctgc cagtactgga gttgcgccgc
    47641 cggatgggct atgccattca atctattggc ctgttccccc actggagcgt ggcgcaaaac
    47701 atcgctaccg tgccgcaatt acaaaaatgg tcgcgggcgc ggattgacga tcgtatcgac
    47761 gaattaatgg cgctactggg gctggagtca aatttgcgtg agcgttatcc gcatcagctt
    47821 tccggtggtc agcagcaacg tgtgggagtg gcgcgtgcac tggctgccga tccgcaagtc
    47881 ttactgatgg atgaaccttt tggcgcactg gacccggtaa cgcgcggcgc gttgcaacaa
    47941 gagatgacgc gcattcaccg tttgctgggg cgcactattg tgctggtcac tcatgatatt
    48001 gatgaggcgc tacggctggc agaacatctg gtattgatgg atcacggtga agtggtgcag
    48061 caggggaatc cgctgacgat gctgactcgt ccggcgaatg attttgtccg ccagtttttt
    48121 ggacgtagtg aactgggggt gcacctgctt tcgttacgta gcgtggcgga ttacgtgcgt
    48181 cgcgaagaac gggcagaagg tgaggcactg gcagaagaga tgacgctacg cgatgcgctc
    48241 tccctgtttg tcgcgcgggg atgcgaggtg ctgccggtgg tgaacacgca gggccagcct
    48301 tgcggcacgc tgcattttca ggatctgctg gtggaggcgt aagcgtatga agatgttgcg
    48361 cgatccgctg ttctggctca ttgctctttt tgtggcgctg attttctggc tgccttacag
    48421 ccagccgctg tttgctgcct tgttcccaca actgccacga cccgtttatc agcaagaaag
    48481 ttttgcagct ctggcactgg ctcatttctg gctggtggga atttcgagtt tgtttgcggt
    48541 gatcattggc acaggtgccg gaattgctgt tactcgcccg tggggcgcgg aatttcgccc
    48601 actggtggaa actattgccg ccgttggaca gacttttccg cctgtcgcag tgctggcgat
    48661 tgccgttccg gtgatcggct ttggtctgca accagcgatt atcgccttga tcctttacgg
    48721 tgtgctgccc atcctgcagg tgacacttgc cgggctggga gcgattgatc ccagcgtgac
    48781 agaagttgcg aaaggtatgg gaatgagtcg aggtcagcga ctgcgtaagg tcgagctacc
    48841 gctggcggct ccggtgattc tggcgggcgt gcgaacttcg gtgattatca acattggtac
    48901 ggcgacgatc gcctcaacgg taggggccag tacgctgggt acgcccatca tcatcgggct
    48961 tagcggattt aataccgcgt atgtgatcca gggggcgtta ctggtggcac tggcggcgat
    49021 catcgcagac cgcctgtttg aaaggctggt gcaggcgctt agccagcacg caaaataaag
    49081 gtataacctg cgagcatgac gccaccaatt ccgcctaacg ccataaacag gaacagggcg
    49141 atgaccccaa ttttagctat gcgcataatg cactccttat gttaacgaaa ggattgtaca
    49201 gtaaagcgca tttgttaacg aatcattaaa taccgagtgg gaaaatatca tggccttgtt
    49261 cttgccaact ggtgagttgc tgctgttggg cggaggttcg attttcaccg caccacacca
    49321 gcaatgtacg gccttcgaat agttcagggc gtagttgatt gagcgagtgg gcgagcgagg
    49381 acatcaatgc gccatccttg ttgactggca atccagccct ccagccacag acgggtggta
    49441 tcctgaatat tccagccaac caccagcgca tctttaccct gttttttacg tgccgaagcc
    49501 agacaaatgg cgatgtagtt gatcagtacg ccgtcgagga tcgccagcag cgcctggaga
    49561 gtcggttgtt ggcactgaag ccgtcggcgc agaggaataa acagatgtgt ggtgagtgtc
    49621 tgggcggggt aatcctgacc gcgctctttg atccacgttc gcaggctatg tagattgccg
    49681 ctgaaccgcc ccgggtttcc tggagagtgt tttatctgtg aactcaggct gccagatcat
    49741 cgtttccgat ggaagcataa taagcttttt ctgcttctgc cggagggatg tggcccagcc
    49801 tttccagcaa tcgtcgattg ttataccagt ccacccacgt gagtgtggcc agttccactt
    49861 ctgcacggtt tttccagctc ttacggtgta ttacctccg
//
LOCUS       DAETQL010000023        48331 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.023, whole genome shotgun sequence.
ACCESSION   DAETQL010000023 DAETQL010000000
VERSION     DAETQL010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.023"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            187..480
                     /locus_tag="K0W65_001416"
     CDS             187..480
                     /locus_tag="K0W65_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBI3094733.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="K0W65_001417"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="K0W65_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HBI3094734.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="K0W65_001418"
     CDS             complement(1922..2374)
                     /locus_tag="K0W65_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBI3094735.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="K0W65_001419"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="K0W65_001419"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HBI3094736.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="K0W65_001420"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="K0W65_001420"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBI3094737.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="K0W65_001421"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="K0W65_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBI3094738.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="K0W65_001422"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="K0W65_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBI3094739.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="K0W65_001423"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="K0W65_001423"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBI3094740.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="K0W65_001424"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="K0W65_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBI3094741.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="K0W65_001425"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="K0W65_001425"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBI3094742.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="K0W65_001426"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="K0W65_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBI3094743.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="K0W65_001427"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="K0W65_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBI3094744.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="K0W65_001428"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="K0W65_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HBI3094745.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="K0W65_001429"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="K0W65_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBI3094746.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="K0W65_001430"
     CDS             complement(15085..15582)
                     /locus_tag="K0W65_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBI3094747.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="K0W65_001431"
     CDS             complement(15579..15941)
                     /locus_tag="K0W65_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBI3094748.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="K0W65_001432"
     CDS             complement(15931..16278)
                     /locus_tag="K0W65_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HBI3094749.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="K0W65_001433"
     CDS             16387..16800
                     /locus_tag="K0W65_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094750.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="K0W65_001434"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="K0W65_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="K0W65_001435"
     CDS             complement(18324..20039)
                     /locus_tag="K0W65_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HBI3094751.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="K0W65_001436"
     CDS             20206..21099
                     /locus_tag="K0W65_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBI3094752.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="K0W65_001437"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="K0W65_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23526)
                     /locus_tag="K0W65_001438"
     CDS             complement(21910..23526)
                     /locus_tag="K0W65_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBI3094753.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFLEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23821..24495
                     /locus_tag="K0W65_001439"
     CDS             23821..24495
                     /locus_tag="K0W65_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBI3094754.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24534..26195)
                     /locus_tag="K0W65_001440"
     CDS             complement(24534..26195)
                     /locus_tag="K0W65_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBI3094755.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="K0W65_001441"
     CDS             complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="K0W65_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBI3094756.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="K0W65_001442"
     CDS             complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="K0W65_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBI3094757.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27338..27410)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27649..27981
                     /locus_tag="K0W65_001443"
     CDS             27649..27981
                     /locus_tag="K0W65_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBI3094758.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27983..29221)
                     /locus_tag="K0W65_001444"
     CDS             complement(27983..29221)
                     /locus_tag="K0W65_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HBI3094759.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTNTWTPDSQWLVFDVRPSGAS
                     FTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFH
                     HRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLD
                     PALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGW
                     VGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVV
                     QRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKL
                     THNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSP
                     DGQWLAWMEGGQLWITETDR"
     gene            29322..30422
                     /gene="cbrA"
                     /locus_tag="K0W65_001445"
     CDS             29322..30422
                     /gene="cbrA"
                     /locus_tag="K0W65_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HBI3094760.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30383..31720)
                     /locus_tag="K0W65_001446"
     CDS             complement(30383..31720)
                     /locus_tag="K0W65_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3094761.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALCFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31795..32943)
                     /gene="dgoD"
                     /locus_tag="K0W65_001447"
                     /pseudo
     CDS             complement(31795..32943)
                     /gene="dgoD"
                     /locus_tag="K0W65_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32940..33557)
                     /gene="dgoA"
                     /locus_tag="K0W65_001448"
     CDS             complement(32940..33557)
                     /gene="dgoA"
                     /locus_tag="K0W65_001448"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBI3094762.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33541..34419)
                     /gene="dgoK"
                     /locus_tag="K0W65_001449"
     CDS             complement(33541..34419)
                     /gene="dgoK"
                     /locus_tag="K0W65_001449"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBI3094763.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34416..35105)
                     /gene="dgoR"
                     /locus_tag="K0W65_001450"
     CDS             complement(34416..35105)
                     /gene="dgoR"
                     /locus_tag="K0W65_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBI3094764.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35383..36039
                     /locus_tag="K0W65_001451"
     CDS             35383..36039
                     /locus_tag="K0W65_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094765.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36085..36897)
                     /gene="yidA"
                     /locus_tag="K0W65_001452"
     CDS             complement(36085..36897)
                     /gene="yidA"
                     /locus_tag="K0W65_001452"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBI3094766.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37011..37409)
                     /locus_tag="K0W65_001453"
     CDS             complement(37011..37409)
                     /locus_tag="K0W65_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBI3094767.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37649..40063)
                     /gene="gyrB"
                     /locus_tag="K0W65_001454"
     CDS             complement(37649..40063)
                     /gene="gyrB"
                     /locus_tag="K0W65_001454"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBI3094768.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40092..41165)
                     /gene="recF"
                     /locus_tag="K0W65_001455"
     CDS             complement(40092..41165)
                     /gene="recF"
                     /locus_tag="K0W65_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBI3094769.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41165..42265)
                     /gene="dnaN"
                     /locus_tag="K0W65_001456"
     CDS             complement(41165..42265)
                     /gene="dnaN"
                     /locus_tag="K0W65_001456"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBI3094770.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42270..43673)
                     /gene="dnaA"
                     /locus_tag="K0W65_001457"
     CDS             complement(42270..43673)
                     /gene="dnaA"
                     /locus_tag="K0W65_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBI3094771.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43970..44050
                     /locus_tag="K0W65_001458"
     CDS             43970..44050
                     /locus_tag="K0W65_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094772.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44280..44420
                     /gene="rpmH"
                     /locus_tag="K0W65_001459"
     CDS             44280..44420
                     /gene="rpmH"
                     /locus_tag="K0W65_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBI3094773.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44437..44796
                     /gene="rnpA"
                     /locus_tag="K0W65_001460"
     CDS             44437..44796
                     /gene="rnpA"
                     /locus_tag="K0W65_001460"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBI3094774.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44760..45017
                     /gene="yidD"
                     /locus_tag="K0W65_001461"
     CDS             44760..45017
                     /gene="yidD"
                     /locus_tag="K0W65_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBI3094775.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45020..46666
                     /gene="yidC"
                     /locus_tag="K0W65_001462"
     CDS             45020..46666
                     /gene="yidC"
                     /locus_tag="K0W65_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBI3094776.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46772..48136
                     /gene="mnmE"
                     /locus_tag="K0W65_001463"
     CDS             46772..48136
                     /gene="mnmE"
                     /locus_tag="K0W65_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBI3094777.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11987 a        13062 c        12220 g        11062 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaactt ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagcaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 acggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa gcttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccgcc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttcg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcagaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat ttccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccag gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaatg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc
    23461 ggcgaaggga aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact
    23521 gagcatttcc acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg
    23581 cagacctgga gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc
    23641 gtgatcgctt tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga
    23701 catctgtgag cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat
    23761 cgattaagca gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag
    23821 atgatctaca aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg
    23881 ctaaacagcc ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg
    23941 actatccgta aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc
    24001 agcggcactt acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg
    24061 gtggaggtgt taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc
    24121 atgcctgcgc ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac
    24181 ttctcccgtc gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg
    24241 ccggtaaaac tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat
    24301 attgaacaag agtgcgggat tttgattggc ggtaattatg aaagcctgat gccggtgctc
    24361 gccgatagac tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc
    24421 acctcgttgt catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat
    24481 gccagcgaat actaggtgga gtaccattta cggcgactcc acccggaaaa aagttaaccg
    24541 atactccaga agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac
    24601 ggatagacag tggcgtaaga gagcgccgcc gcaccactgg ttggatgaag attattagca
    24661 aacgccagcg ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa
    24721 ttcattttgg ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc
    24781 agggcaccat aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc
    24841 ccaccagatt tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcgcg
    24901 aggttggcac ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg
    24961 atcaacgcca taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct
    25021 ggcacaaaga cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc
    25081 agcatctgaa cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca
    25141 attgcggacg gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca
    25201 ctggcgacca gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct
    25261 ttaaagtgca ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc
    25321 tcgacgcgca aatcagtgcc tttcgtagac agcgaggtat cgacctcctg accaatcacc
    25381 agttgcgcat tatgtaaatc cgctggctgc cccaccagat gcagcaaatc gcccagttgg
    25441 ataatggtat ctggcgaagg tacttttagg gtttcttctc gtttcagacg cgagcagata
    25501 attttgtcgc cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc
    25561 tcaacgcgaa tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc
    25621 tgctgagctt ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac
    25681 aaaatcccgc aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc
    25741 atttccattg gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta
    25801 ttggttaccg caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta
    25861 tgcaggatgg cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg
    25921 cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta
    25981 taaacgaaca ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc
    26041 atccccgcct gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata
    26101 cctaatccta tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa
    26161 gccagaatac tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt
    26221 ttaattgcta cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat
    26281 cacgggaaag aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa
    26341 gaaagacaaa aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt
    26401 aacgcgggac gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga
    26461 attaaatcaa tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca
    26521 gccagcgtaa aactcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc
    26581 tcttcttttg gctgctccgg cgtgcctttt acgctcaggc gcgtgccttc cagttgaatc
    26641 tctaaatctt cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg
    26701 cttttctcaa tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg
    26761 gccagtttgt caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata
    26821 gtcatttctc cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc
    26881 cctgacggcg agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt
    26941 tcgatacggc gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa
    27001 ccatttacca ggttagcccc acgaacatga atgttctcag ctaactggaa tttgcgttca
    27061 aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga
    27121 gcacctttca ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa
    27181 ccagccacag cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga
    27241 gggtagccgc cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat
    27301 ccaatagcag aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga
    27361 aatcagcgag aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca
    27421 ggcgcgtcct catcgactac gatgtaaaat gggtctggaa atgaactttt caagccttat
    27481 cagcggactt ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac
    27541 gttaaagtgc gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat
    27601 cgctataact catcaggcca acacccacga tcaacagatg aatttattat gataagaaat
    27661 gtgttgttag cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg
    27721 atgtcccata ccggggggaa agaaggaacg tatcccggca cccgcgccag cgccaccatg
    27781 attggcgacg atgaaaccaa ctggggcact aagtcgctgg cgattctcga tatgccgttt
    27841 accgctgtaa tggacacgct tttactgccg tgggatgtgt tccgcaaaga cagctcggtg
    27901 cgctcgcgcg ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt tattccaccg
    27961 gccagaatgc ctgataactg atttagcgat cagtttcggt gatccacagc tggccacctt
    28021 ccatccacgc cagccattga ccatccggcg agaagaccac ggcgtccgca gaaggtggat
    28081 tggcgtggtt ttcggttaaa tactcaacct cgccgctttg cgcatgggca caaacaattc
    28141 gattatccag cacaaagccc aaccattctc ctgacggatg ccagttaaat gcagactgaa
    28201 tatccgtttt gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca
    28261 gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac
    28321 agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg
    28381 ttaaacgtcg ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta
    28441 acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata
    28501 gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat
    28561 ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg
    28621 gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct
    28681 tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg
    28741 gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc
    28801 taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat
    28861 ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga
    28921 agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg
    28981 acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct
    29041 cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag
    29101 aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtattggtat
    29161 tggtattggt attggtattg gtgagcaggt gattacgggg agcaaaggtt atctgtttca
    29221 tggcgcggtc ctgatacaaa acggttcccg ccatcatact tcacaaggac ttcactttca
    29281 gacaatagtc ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat ttcgacgtgg
    29341 cgattattgg cctcggcccg gcagggtcgg cgttggcacg aaagttagcc ggcaaaatgc
    29401 aggtgatcgc gctggataaa aagcaccagt gcggtactga aggtttcagc aaaccttgcg
    29461 gcggtctgct ggcaccggac gcgcagcgat cttttattcg cgatggactg acgcttcctg
    29521 tcgatgtgat cgccaatcca cagattttca gcgtcaaaac tgtcgacgtc gccgcatcgc
    29581 tcacgcgtaa ctaccagcga agctatatca atattaatcg ccatgctttc gacttgtgga
    29641 tgaaatcgct gatccccgcc agcgttgagg tttatcacga tagcctgtgc cgaaaaatct
    29701 ggcgtgagga tgataaatgg catgtcattt ttcgtgcaga cggctgggag caacatatta
    29761 ccgcccgcta tctggtcggt gccgatggcg caaactcgat ggtgcggcga catctctacc
    29821 cggatcatca aatccgtaaa tatgtcgcta tccagcagtg gttcgcagag aaacatccgg
    29881 tgccgttcta ctcctgcatc tttgataatg cgataactga ctgttactca tggagtatca
    29941 gcaaagatgg ttattttatc tttggcggtg cctatccaat gaaagacggt cagacgcgtt
    30001 tcacgacgct gaaagagaaa atgagcgcct ttcagttcca gtttggcaaa gcggtgaaaa
    30061 gtgagaaatg cacggtgttg tttccctcac gctggcagga ttttgtctgt ggtaaggaca
    30121 acgcctttct gattggtgaa gcggcgggat ttatcagcgc cagctcgctg gaggggatta
    30181 gctatgcgct ggatagcgca gagattctgc gtgcggtgtt cctgaaacag ccggagaaga
    30241 gcaacgccgc ttactggcgc gccacacaca aactgcgttt aaaactcttc ggcaagatag
    30301 taaaaagccg atgcctgacc gcaccggctt taagaaagtg gattatgcgc agtggtgtgg
    30361 cgcatattcc acagttgaaa gattatccaa cgcgcttcac atcacccacc agcaggatgt
    30421 aagagagcgc gccaatcaac gcgacggcgg agatataaac cagtgcaggc gcgaaaccgt
    30481 aaccctgcgc caggtacccc accaccagcg gaacggtgat gccgcccaga ccaccggcga
    30541 agttaaacac gccgccggtt aaaccaatca ggcgcatcgg tgccagagaa gagaccagcg
    30601 accaggtaat cgaagcaaaa ccgttgccga agaatgccag cgccatcagg cacataatca
    30661 tcatcggatc gttggtgtag ttagcgccca taatgcaggt ggagatcagc aagccgcaga
    30721 taatcggcgt tttacgcgca aagcccagtg aaaagccctt acgtaccagc agatccgcga
    30781 cccagccaga gagcaggacg ccgacaaacg ccgcgaggaa tggcaccgtg gtcataaagc
    30841 ctgctttcag cgccgtgatt cctttttcct gggttaaata gttcgggaac caggttaaga
    30901 aaaaacacag tgcagaagcc accgcaaatt gcccaagata aacgccgatc agtttacgat
    30961 ggaacaccag tttccagtct ttggctgtta acggctgacg cgcctctttc ttcaccggcg
    31021 catcaccatc caccagaccg ccgccatcac gaatgtaatc cagttcagct ttgctgatac
    31081 ctttggtcag gcgcggcggc tgataaacct taaaccaaat cagcgaccag ataatgccga
    31141 taccaccagt gacaatgaac acccagtgcc agctcaacat ctcctgaatc cagatcagca
    31201 gcggagtcaa aaacgccaga ccgacaaact gaccagacgt ataaaaacca acggcggaag
    31261 cgcgttcatg ttccgggaac cagctggtca ccatccggtt attggtcggg aaagcgggcg
    31321 cttcgaaaat accggttatc gcacgcagac caattaatga cattaatccc gtggcaaagc
    31381 cttggaataa agtcgccact gaccaaccaa atatcgcaat aaaataagtc acgcgagaac
    31441 ctacgcgatc taaaaaccaa ccgccgggga tctggcatag cgtataaagc caggcgaagg
    31501 ccgaaaatac atagcccatt tccgctttcg taatgccgaa ctcttcctga atatgggcgg
    31561 aagccacggc caggttagcg cggtcaacat aacaaatgac caccgtaata aagatcatca
    31621 ccagcgtcag ataacgccga cgccccggct ttgctgcatt aacgggaata tccatagcga
    31681 gctttctcca gattttgggc atagcgaagc cgctcaccat gccctgtaat ttacagaggg
    31741 ttatattttt gtattgctgt tttagtgccc gatgaggggc ttacgtggca ggaattacca
    31801 ctctgctacg ctgttatctt catgacgcca gagcggatta cgccagtccg gggcattttt
    31861 actgaactca ataactttag cttcgtcgat ttccacacct aagcccggtt tcgttaacgg
    31921 tttaaagaag ccgccaacca tgctgaagtc ttctttgttt ttcacaaagt cgagtaactc
    31981 cgcgcctttg ttgtaatgaa tgcccatact ttgttactga agtaccgcgt tataggaaac
    32041 aaagtcgata tgcaggcaag ccgccagtgc aatcggtccg aacggacagt gcggcgcaag
    32101 ggtcacatca taggcttctg ccattccagc aattttgtag cattcggtaa taccgcctgc
    32161 atgggagaga tccggttgca gaatcgaaat accgcctgcc tccagcacgc gtttaaaatc
    32221 gaaacgtgag aacatgcgct cacccgccgc cagtggaata tgcgtttgtg ccgccaattt
    32281 cgggtagtat tcggcttgtt ccgccagcac cggctcttca ataaacagcg ggcgatacgg
    32341 ctccagttct ttaatcagca ctttcgccat tggcgcgctg acgcgaccgt ggaaatcaag
    32401 accaaactca atctgattgc caaaagcttc acgaatttgt gccacggtgt tgactgccgc
    32461 atctaccgcg cgggagttat caattagccc cagttcttca caaccgttca gtttgaaggt
    32521 atcgaagccg atttcgcgca gggttttaat gccgtcgata acatccgccg gacgatcgcc
    32581 gccgacccaa ctgtaggctt taattttgtc gcgaaccagg ccgcccatca gttgccagac
    32641 tggcgcattc agcactttgc ctttgatatc ccataacgcc tggtcgatcc cggcgatggc
    32701 gctcatcagg attggaccgc cacgataaaa tccggcgcga tacatcactt gccataagtc
    32761 gttgatgcgc gaaggatcct gaccaatcaa atagtcaccc agctcgtgaa ctgccgcttc
    32821 caccgtacgg gcgcggcctt caatcacggg ctcgccccaa ccgaccacgc cttcatcggt
    32881 ttcaattttc aggaacatcc agcggggagg taaacgatac gtggtaattt tggtgatttt
    32941 cattgcactg cctctcgata cgccttaaca aatgctgctg cctgctgcgc ggtgcgttct
    33001 acggactgcc cggcgcgata aagatcgctg cctaagcccg ccccaacaca acctgcgtct
    33061 atccactgcg ccaggttttc tggcgtcacg ccgccaacgg caaagactgc gatgtccggt
    33121 ggtaataccg cttttaacgc tttgatgtat tgcggaccaa aagccgatga cggaaatatt
    33181 ttcagcgcct gcgcgcccgc ttcgagcgcg gtaaaggctt cggtcgccgt cgcgcagccg
    33241 gggcaaacgg tcatgccgta gcccaccgcg cggcggatca cttcactatg gatattgggc
    33301 gtaacgatga gctgacagcc catcctggcg agcgcatcga cctgttcagg tttcagtacc
    33361 gtacctgcgc caatcaacgc cttgtcgcca tacgcatcaa cgatggcggg aatgctctgc
    33421 tcccattgtg gggaattcag cgggatttca accgcgtcga acccggcgtc aatcaccgcg
    33481 ccaacatgcg ccagcgcctc gtcgggcgta atgccgcgca aaatggcgat cagcgggagt
    33541 ttagtttgcc actgcatgag cgatgctcct tataccagcc tgaaatgccg tgtcgcccgc
    33601 caccgccgcc acgtcgcaac ccatcgcctg aaaggcttgc tggtagcgcg cggtcagcga
    33661 tgttccggcg acaagggtga tggcgtgttg atgggtcaca tagtcgcgca tactggcaac
    33721 ctctgcgcca atcaacaaac cagagagaaa ttcgctgacc tgttcgcggg gaagtgttcc
    33781 cagcacatgc gaggcgcgaa cttcaaaaag ctgcggcaat atggcgggcg cattaaggcc
    33841 gcgctcaagg ccagccgcga aggcatcggc agagttttcc tgcggcggca aacctgcgcc
    33901 aatcaatgag tgatttaaca gtaaatgatg taattcaccg gtcatcacgg tgcgaaaatc
    33961 gttgatttgc tggctatcgg cctgcaccca tttgcaatgc gtaccgggca taacataaag
    34021 agaggaagga gccagagctc gcgcgccgat caattgtgtt tcttcgccgc gcatcacatt
    34081 gtggttatcg tcatgagaga cgcataatcc gggaataatc cagatattgt cgccaacaga
    34141 cgttaattgt tcgccaatag acgaaaaacg ggcaggaaca gataaatacg gagcaacttt
    34201 ccagccgaca ttgctgccga ccattcctgc catcaccact ggcgtatttt cttcacgcca
    34261 gtcggtcgtg acttctgcta acactgcagc cggagatttt ccgttcaggc gcgtgacgcc
    34321 tgcttctgat tgcctgctct ccaggcagtg gtcgccctga taaagccagg cgcgcagatt
    34381 ggtcgatccc cagtcaattg cgatgtagcg agctgtcatg tgatttcctt taaccttcgt
    34441 gtcgagctgg caatcatggt aagcgccgcc tgctctgccg catcgccgtc ctgatgccgt
    34501 atcgcatcga acagcgcctt atgttcctgg agcgtttgcg gcatgttggc ctcatcgccc
    34561 atccaggttc gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct aagttgctgt
    34621 aacaccgggt tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc cgcttcgtta
    34681 aacgcttcgc ggtcctgatt gttggcaatc atctcgttca gcgccgattc aatctgcgcc
    34741 agatcgctgg aggtcgcgcg ctctgctgcc caacgggcaa tcgccggttc caccagattt
    34801 cgcacttcgc tcatggcact gataagccgt gggtcgtagt cgttttccag cacccattgc
    34861 agtacgtcag tgtcgaggta attccactgg ttacgcggtg ccacaaacgc cccgcgataa
    34921 cgtttcattt caatcagccg cttcgccatc agcgaacgga acacctcacg gatgatgttg
    34981 cgcgaggttg caaactcttc acagagctcc gcctcagccg gaagtggcga gccaggtacg
    35041 tatttgccgt gaacgatctg tttacccagc gtaatgacaa tgcgatcggt tttattgaga
    35101 gtcatggaga gtccttgtgc tctgtatgtt cttctctact ttaccccgat cgatgcataa
    35161 cgcggcaact ttgtagtacc agcgtgatga cgttcgcgtt tgccgcgcgc gtaatgtagt
    35221 acaaacttat attattgcgc tacaatttag atcaaaaaaa gaacaacaca taaaaaaatg
    35281 acatgcgtcg ggcagaaatc tgaaaaggga tatcaggcgc taaacaggag ggaaagaaga
    35341 gtatgctttc aacggcttag ctactcgttt aaaggattaa tcatgaagtt gaattttaag
    35401 ggatttttta aggctgccgg tttattccca ctggcgctga tgctttcagg ctgtatctcg
    35461 tatgctctgg tttcccatac cgcaaagggt agttcaggaa agtatcaatc gcagtcagac
    35521 accatcactg ggctatcgca ggcaaaagat agtaatggaa caaaaggcta tgtttttgta
    35581 ggggaatcgc tggattacct tatcactgat ggtgccgatg acatcgttaa gatgctcaat
    35641 gatccagcac ttaaccggca caatattcag gttgccgatg acgcaagatt tgttttaaat
    35701 gcggggaaaa agaaatttac cggcacaata tcgctttact actactggaa taacgaagaa
    35761 gaaaaggcac tggcaacgca ttatggtttt gcctgtggtg ttcaacactg taccaggtca
    35821 ctggaaaacc taaaaggcac aatccatgag aaaaataaaa acatggatta ctcaaaggtg
    35881 atggcgttct accatccatt taaagtgcga ttttatgaat actattcacc cagaggcatt
    35941 ccggatggtg tttccgcagc attactgcca gtgactgtta cactggacat cattactgca
    36001 ccgctgcaat ttctggttgt atatgcagta aaccaataat cagtaagcgg gcaaacgcgt
    36061 ttatgctgtt tgcccgccca cagattaatt cagcacatac ttctcaatag caaacgccac
    36121 gccatcttca aggttggatt ttgtgacaaa gttcgccact tctttcaccg aaggaatagc
    36181 gttatccatc gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt tttcctgatc
    36241 gccaatcgcc atgatttctt ccggtttaat acctaacacg tcggccagtg atttcacacc
    36301 cgtacctttg ttaacgcgtt tatcgaggat ttcgaggaag tacggcgcac ttttcagcac
    36361 ggtatatttc tctttcactt cctgcggaat acgcgcgata gcctggtcga ggatggcggg
    36421 ttcatcaatc atcatcactt tcaggaactg ggtattgggg tccattttct ccgcttcgca
    36481 gaacaccagc ggaattgtgg caacgaagga ttcatgcacc gtgtagtagc tgatatcgcg
    36541 gttagcggtg tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga cttcgcgaga
    36601 gagtttttcc aggaaacgat agtcgtcata gctgagagcg gtttgcgcca cggtgctacc
    36661 atcagcggcc ttctgtacca gcgcgccgtt ataagtaatg cagtagtcgc ccggctgttc
    36721 catatgcagc tctttcaggt agttgtgcac acctgcatac ggacgacccg tcgttagcac
    36781 gacattcacg ccacgggcgc gagctgcggc aatcgcattt ttaacggcgg gtgaaatggt
    36841 gtgatcgggc agcagaaggg tgccatccat atcgatagca atgagtttga tagccatgag
    36901 ttcccaaggt agattagttc ctgacccatg ctaacgcgat tccgctcaaa aatcagtaca
    36961 acacccgagg gaaaagggga tgcaacgcgg gttgcatgct ccccatttgc ttagcggaag
    37021 agtttccctt tcagcagttc catgcctgcg gaaagcagat cgttattggc ttgtgctgac
    37081 acttcacctt gcggtgagag cgcatcaata atcttcggca attgctctgc cagtaaactg
    37141 gaagccgtac tggtatccac gccaagtttt tgcccgagat cggacaccgc atttgtgccg
    37201 agcgccgact ccagttgctc gccgctaact gattgatttc tctgttgatt actcaaccag
    37261 gttgagagaa tggcccctaa gccgccactt tgcagttttt ccagcagcac ctgaatgcca
    37321 ccctgctcct caacccaact taaaatcgcc tgatatttcc ccgcatcgcc tttcagaaag
    37381 gcaccgacaa cttcatcaaa aagccccatg ataatcacct gtaaagcgtt acgtgttgac
    37441 ccaaaaagta tagatctgcg ggatgataat tgcggattgc agaaataaaa agggcggaga
    37501 tgatctccgc ccttttctta tagcttcttg ccggatgcgg cgtgaacgcc ttacccggcc
    37561 tacaaaatca tgcaaaatca atatattgca ggagtctcgt aggactaata agcgtagcgc
    37621 atcaggcacg ctcgcatggt taacgccatt aaatatcaat attcgccgct ttcagggcgt
    37681 tctcttcaat aaacgcacga cgcggttcta cggcgtcgcc catcagcgtg gtgaacaact
    37741 ggtcagcggc aatcgcatcc ttaacggtaa cgcgcagcat acgacggctt tccgggtcca
    37801 tggtggtttc ccacagctgt tccgggttca tctcgcccag acctttataa cgctggatgg
    37861 agaggccacg acgggactct ttcaccagcc agtccagcgc ctgctcgaag ctggctaccg
    37921 gctgacgacg ctcgccacgt tcgataaacg catcttcttc cagcaagcca cgcagtttct
    37981 cacccagcgt gcagatacga cgatattcgc caccggtgat aaactcgtga tccagcggat
    38041 agtcagtatc cacaccgtgg gtacgcacgc gaacaatcgg ctcgaacagg ttttgttcgg
    38101 cattggtgtg gacatcaaac ttccactggc tgccgtgctg ttctttgtcg ttcagttcgc
    38161 tgaccagcgc gttcacccag cgggtaacgg tctgctcatc agaaaggtca gcttccgtca
    38221 acgtcggctg atagataagc tctttcagca ttgctttcgg ataacgacgc tccatgcggt
    38281 tgatcatttt ctgcgtcgcg ttgtactcag ataccagttt ctctaacgct tcgccagcca
    38341 atgccggtgc actggcgttg gtgtgcagcg ttgcgccgtc cagcgcgata gagatctggt
    38401 actgatccat cgcttcgtcg tctttaatgt actgttcctg cttgcctttc ttcactttgt
    38461 acagcggcgg ctgagcgatg tagacgtgac cgcgttcaac gatttccggc atctgacgat
    38521 agaagaaggt caacagcagc gtacgaatgt gcgagccgtc gacgtccgca tcggtcatga
    38581 tgatgatgct gtgataacgc agtttgtccg ggttgtactc gtcacgaccg ataccacagc
    38641 caagcgcggt gataagcgtc gccacttcct gagaagagag catcttatcg aagcgcgctt
    38701 tctcgacgtt aaggatttta cccttcagcg gcagaatcgc ctggttcttg cggttacgcc
    38761 cctgcttcgc agagccgccc gcggagtccc cttccaccag gtacagttcg gaaagcgccg
    38821 gatcgcgttc ctggcagtct gccagtttgc ccggcaggcc agctaagtcg agcgcacctt
    38881 tacggcgggt catttcacgc gcgcgacgcg ccgcttcacg ggcacgggca gcatcgataa
    38941 ttttgccaac cacgattttc gcatcagttg ggttttccag caggtattcc gccagcagtt
    39001 cgttcatctg ctgttcaacc gccgatttca cctcagaaga aaccagtttg tctttggtct
    39061 gggaggagaa tttcgggtcc ggcactttta cggaaacgac cgcaatcagg ccttcacgcg
    39121 catcgtcacc ggtggcgctg actttggctt ttttgctgta gccttctttg tccatgtagg
    39181 cattcagggt acgggtcatc gccgcacgga agcctgccag gtgagtaccg ccgtcacgct
    39241 gcggaatgtt gttggtaaag cagtagatgt tttcctggaa gccatcgttc cactgcagcg
    39301 ccacttcaac gccaataccg tctttttcgg tggagaagta gaagatattt gggtggatcg
    39361 gcgttttgtt cttgttcaga tattcaacga acgctttgat gccgccttca tagtggaagt
    39421 ggtcttcttt gccgtcacgc ttgtcgcgca gacgaatgga aacgccggag ttgaggaacg
    39481 acaactcacg cagacgtttc gccagaattt catattcgaa ctcggtcaca ttggtgaagg
    39541 tttcgaggct gggccagaaa cgcaccatgg tgccggtttt ttcagtctcg ccggtaaccg
    39601 ccagcggagc ctgcggtaca ccgtgttcgt agatctgacg gtgaatttta ccctcgcgct
    39661 gaataaccag ctccagtttt tgcgacaggg cgtttactac cgaaacacca acgccgtgca
    39721 gaccgccgga cactttatag gagttatcgt caaatttacc gcctgcgtgc agaacggtca
    39781 tgatcacttc cgccgccgat acgccctctt ccgggtgaat accggtcgga atgccgcgcc
    39841 cgtcatcctg tacagagaca gagttatcgg cgtgaatggt gacgataatt tctttacagt
    39901 gacccgcgag cgcttcgtcg atagcgttat ctaccacctc gaataccatg tggtgcagac
    39961 cggtgccgtc atccgtgtcg ccgatataca tacccgggcg cttacgcacc gcatccagcc
    40021 ctttcaggac tttgatactg gaggagtcat aagaattcga catcaacgtt tctcgctcat
    40081 ttatacttgg gttaatccgt tattttaccc ttttccacgg taaacatctt cgaattttcg
    40141 tccgacatgt ctataacgtg ttcagcactg atcgcgctga caaagacctg tgattgcgtc
    40201 gcttttaagc ggctggcaag caacccgcga cgctcatcat caagctcaga ggcaaaatca
    40261 tctatcaggt agagacaccg ccgcccgctt tcacgagtga ggaactctcc ttgcgccaga
    40321 cgtaaggcgc acatcaacag cttaagctgc ccacgcgata aggtatcttc caccggcgca
    40381 ccgtcggcgc gaatgcgtaa gtccgcttta tgcgggccat gcgcggtgta ggttagctgg
    40441 cgatcgcgtt caaaattacg ttccagcacc tcagcgtact ctgtctcttt ctcccagccg
    40501 cgctggaaag agaaagtcag agaaaactca gggagaaatt gcttacaggt atcatccata
    40561 tcggccgcga taccggcgct atactccgcg cgccaggtgc tgatttgctc cgccagcggg
    40621 atcagttctt tatcccacgg gcgtagctgt tcgtaacgag tcacctggcg cagcgccgca
    40681 ttgcgctgct tgagcaatcg cttgagattg ctccaggcgg tgaaaaatcc aggttcgttg
    40741 tgaaagcatc cccagtcgag gaatgctctt ctgtatttgg ggccgccgtt gagtaaagta
    40801 aacccttctg gcgttatcaa ctgcatcggc atcaggtgcg ccagttccgc gactttatgc
    40861 ccgtccgtac cgtcaatacg gactttgctg tcgccctgtt tgtctttggt taagccaatc
    40921 gccgtctcgc gctcttcgcc ctgtaatcgc ccgtggagaa caaatgcctc ctgctcatgg
    40981 cgaatgacgc gaccaatctg caaactgcga aacgcccgac catggccgag cgtatagatg
    41041 gcttccagca cgctggtttt gccactgccg ttggcaccta ccagaaagtt aaagccggga
    41101 gataaagcga gatccgcggt ttcaatattg cggaaatcgc ggatcaacaa gcgggtgagg
    41161 gacattacag tctcattggc atgacaacat aggcagccga ctgtgatgcg gcatcttcaa
    41221 tctgcacgct ggaaaccgaa tcggtcagca tcatgcggac gttttcgcat ttcagcgcgt
    41281 tcagaacatc cagcacatag ctgacgttga agccgatttc catctccgca ccgctatagg
    41341 taacgtcgag gatctcttcc gcttcttcct gttccgggtt gttggcagta attttcagct
    41401 ggttttcgct gacatacagg cgcacaccgc ggaatttctc gttagagaga atcgccgcgc
    41461 gagcaaacgc ctgcttgagc agatcgcagc ctgcttccag atgtttgtcc gggttcttcg
    41521 gcagaacgcg acggtaatcc gggaagcgac catccaccag tttcgaggtg aaaataaagt
    41581 cgccaacgtg ggcacgaatg ttattgctgc caatctgcac acgcagcaga ttgtcgccgc
    41641 cgtcgagcat acgcatcagt tcaatcacgc ctttacgcgg tacgatcacc gaatggcttg
    41701 gcaaagattg accaattggc attgaacaga ccgccagacg gtggccgtcg gttgccacgg
    41761 tgcgcagttc ttcaccttcg gtttcaaaca gcataccgtt taagtaatag cgaacatcct
    41821 gatgcgccat cgaaaactgg gttgcttcaa tcagacgctt catcgtcgcc tgcggcaggg
    41881 taaattcgac ttcgctctgc cagtcatcga ggttcgggaa atccgccgct ggcagggtag
    41941 acagcgaaaa acggctacgc ccggagcgca ccagcatccg ttcaccttcc agctgcacgg
    42001 caatttccgc gccttcaggc agaccacggc agatatcaaa gaatttgcgc gccggaacgg
    42061 tcgtcgctcc tggctcgtgt ggctgaacca gcgcaacgcg tgccaccatt tccatctcga
    42121 gatcagtacc ggtcagcgac aacgtaccgt cagcaacctg taacagcaga ttaccgagaa
    42181 tcggtagcgt aggacgacca cctaacggac cgctcacctg ttgtagcggt tttaataaat
    42241 gctcacgttc tacggtaaat ttcataggtt tacgatgaca atgttctgat taaatttgaa
    42301 aaatcttctt tgatatcgtg gctctcttca cgcaactgct cgatcttacg gcaggcatga
    42361 agcaccgtcg tgtggtcacg cccaccaaac gcatcgccaa tctctggcag actgtggtta
    42421 gtcagctctt tcgctagcgc catcgccatc tggcgcggac gcgccaccga gcgggatcga
    42481 cgcttggaaa ggagatccgc gactttgatc ttgtagtact ccgccaccgt cttctgaata
    42541 ttgtcgatgg tgaccagttt ttcctgcaat gccagcaagt cgcgcagcgc ctcacgcacg
    42601 aagtcgatgg tgatcgcccg tccggtaaag ttggcgttag caataacgcg gttcagcgcc
    42661 ccttccagct cacgtacgtt agatcgtaga cgcttggcga taaagaacgc cacttcaccc
    42721 ggcaaacgaa tgtcattttc gtcggccttt ttcatcagga tcgccacacg ggtttccagc
    42781 tctggcggtt cgatcgccac agtcagtccc caaccgaagc gggatttcaa acgatcctca
    42841 acgccgttga tctctttcgg atagcgatcc gaggtgagaa tgatctgttg attaccttcc
    42901 agcagggcgt tgaaggtgtg gaaaaactct tcctgagatc gttctttatt agcaaaaaac
    42961 tgaatatcgt cgatcagcag tgcatctacg gaacggtagt agcgtttaaa ctcttcgatc
    43021 gcgttgtttt gcagggcttt aaccatgtcc tgaacaaagc gctcggagtg catataaacc
    43081 actttggcat tcggcttgcg cgccataatg ccgttaccca ccgcatgcag caggtgagtt
    43141 ttacccagac ccgtgccgcc ataaaggaac aacgggttat aggcaccgcc agggttatcc
    43201 gccacctggc aagccgccgc gcgcgccagt tggttagatt taccttcaac gaagttatca
    43261 aacgtgtgtt tgacgtttac gttagaacga taggtcggtt ctgccggggc cgggacgtta
    43321 tcccaacctg agcgcgtaga aggcgcagca cgttgcggct gcgtttgcgc cacctgtgca
    43381 ggggccgcga cgttgctcgt cactgccgct tgtggcgttt gcgtcaccgg tttggtgccg
    43441 acttcaaaac gcagctgtgg ggcatccgct ccgcagaaac tggttagcag tccattgata
    43501 ttattaaggt acttgtcccg tacccaatcg aggacaaaac ggtttggcgc gtacagggcc
    43561 agcgtgttat cgctcagttc cgcctgcaat gggcgtatcc acatactgaa ttctgtggct
    43621 ggtaactcat cctgcaatcg ggcaagacac tgctgccaaa gcgaaagtga cacggcggac
    43681 tccactcgaa caaaagtcga taatgactaa ggctgaaaca ttcatgattg ttgacgtacg
    43741 tcgaaaagac cctgcttgag ggtgacgcac gaaccgctgt ctgcggttat atgcccgatc
    43801 aagatcctgc aaaacgatcg ggaccgcgga tcatagccta aactgcgcaa gagatcttct
    43861 gtttctcaca gattttccct atttatccac aggactttcc agaactcgct aagtgtaaac
    43921 gatcctgccg cgaggcgggc acgatttacg ccgcatattg gaaaatttaa tgaccataga
    43981 caaaaattgg cttaatcgat ctaataaaga tccaggacga tccttgcgct ttacccatca
    44041 gcccgtataa tcctccaccc ggcgcgccat gctggtttcc actggtgtga ggtcgtacat
    44101 tttccctgcg aaaaggtgcg gaaaagcgcg gtaaataagg aaagagaatt gactccggtg
    44161 tgtacaatta ttacaatccg gcctctttaa tcacccatgg cttcggtgtc catcgtttca
    44221 tttttcggcg gatatccaat aaagccattg aatttattca agtttaggta gaaatcgcca
    44281 tgaaacgcac ttttcaaccg tctgtactga agcgcaaccg ttctcacggc ttccgtgctc
    44341 gtatggctac taaaaatggt cgtcaggttc tggcacgtcg tcgtgctaaa ggccgcgctc
    44401 gtctgaccgt ttctaagtaa taaagctaac ccctgagtgg ttaagctcgc atttcccagg
    44461 gagttacgct tgttaactcc cagtcaattc acattcgtct tccagcagcc acaacgggct
    44521 ggcacgccgc aaattaccat tctcggccgc ctgaattcgc tggggcatcc ccgtatcggt
    44581 cttacagtcg ccaagaaaaa cgttcgacgc gcccatgaac gcaatcggat taaacgcctg
    44641 acgcgtgaaa gcttccgtct gcgccaacat gaactcccgg ctatggattt cgtggtggtg
    44701 gcgaaaaaag gggttgccga cctcgataac cgtgctctct cggaagcgtt ggaaaaatta
    44761 tggcgccgcc actgtcgcct ggctcgcggg tcctgatagc cctcattcgg gtctatcaac
    44821 gcctgattag tccgctactc gggccgcatt gtcgtttcac tccaacctgt tcaagctacg
    44881 gaattgaggc attgcgcagg tttggagtga taaaaggcag ttggttgacg gtgaaacgcg
    44941 tattaaaatg ccacccttta caccctggtg gtgacgatcc cgtcccgccc ggaccatttg
    45001 ataccagaga acactaacga tggattcgca acgcaatctt ttagtcatcg ctttgctgtt
    45061 cgtgtctttc atgatctggc aagcctggga gcaggataaa aacccgcaac ctcaggccca
    45121 acagaccacg cagacaacga ccaccgcagc gggtagcgcc gccgaccagg gcgtaccggc
    45181 cagtggccag gggaaactga tctcggttaa gaccgacgtg cttgatctga ccatcaacac
    45241 ccgtggtggt gatgttgagc aagctctgct gcctgcttac ccgaaagagc tgaactctac
    45301 ccagccgttc cagctgttgg aaacttcacc gcagtttatt tatcaggcac agagcggtct
    45361 gaccggtcgt gatggcccgg ataacccggc taacggcccg cgtccgctgt ataacgttga
    45421 aaaagacgct tatgtgctgg ctgaaggtca aaacgaactg caggtgccga tgacgtatac
    45481 cgacgcggca ggcaacacgt ttaccaaaac gtttgtcctg aaacgtggtg attacgctgt
    45541 caacgtcaac tacaacgtgc agaacgctgg cgagaaaccg ctggaaatct cctcgtttgg
    45601 tcagttgaag caatccatca ctctgccacc gcatctcgat accggaagca gcaacttcgc
    45661 actgcacacc ttccgtggcg cggcgtactc cacgcctgac gagaagtatg agaaatacaa
    45721 gttcgatacc attgccgata acgaaaacct gaacatctct tcgaaaggtg gttgggtggc
    45781 gatgctgcaa cagtatttcg cgacggcgtg gatcccgcat aacgacggta ccaacaactt
    45841 ctataccgct aatctgggta acggcatcgt cgctatcggc tataaatctc agccggtact
    45901 ggttcagcct ggtcagactg gcgcgatgaa cagcaccctg tgggttggcc cggaaatcca
    45961 ggacaaaatg gcagctgttg ctccgcacct ggatctgacc gttgattacg gttggttgtg
    46021 gttcatctct cagccgctgt tcaaactgct gaaatggatc catagctttg tgggtaactg
    46081 gggcttctcc attatcatca tcacctttat cgttcgtggc atcatgtacc cgctgaccaa
    46141 agcgcagtac acctccatgg cgaagatgcg tatgctgcag ccgaagattc aggcaatgcg
    46201 tgagcgtctg ggcgatgaca aacagcgtat cagtcaggaa atgatggcgc tgtacaaagc
    46261 tgagaaggtt aacccgctgg gcggctgctt cccgctgctg atccagatgc caatcttcct
    46321 ggcgttgtac tacatgctga tgggttccgt tgaactgcgt caggcaccgt ttgcactgtg
    46381 gatccacgac ctgtcggcac aggacccgta ctacatcctg ccgatcctga tgggcgtaac
    46441 gatgttcttc attcagaaga tgtcgccgac cactgtgacc gacccgatgc agcagaagat
    46501 catgaccttt atgccggtca tcttcaccgt gttcttcctg tggttcccgt caggtctggt
    46561 actgtactat atcgtcagca acctggtaac cattattcag cagcagctga tttaccgtgg
    46621 tctggaaaaa cgtggcctgc atagccgcga gaagaaaaaa tcctgattcg gtgagttttc
    46681 gctaaaataa gggcggtcag ttgaccgcct tttttctttt cgtagggcgg ataagcaccg
    46741 cgcatccgcc acacaaagca acaggaacat catgagcgat aatgacacta tcgtagccca
    46801 ggccacgcct ccgggacgtg gcggcgttgg catcctgcgc atctccggct tcaaagcccg
    46861 tgaagttgcc gaaaccgtgc tgggtaaact gcctaagccg cgctacgccg attatcttcc
    46921 gtttaaagac gccgacggca gcgtgctcga tcaggggatt gcgctatggt tccctggccc
    46981 gaactcgttc accggcgaag atgtgctgga actgcaaggt catggcggtc cggtgatcct
    47041 cgacctgctg ttaaaacgca ttctgaccat tcccggcctg cggattgctc gccctggtga
    47101 gttttccgaa cgcgcgtttc ttaacgataa acttgactta gcccaggccg aggcgattgc
    47161 cgatcttatc gacgccagtt cggaacaggc ggcccgttcg gcacttaact cgctgcaagg
    47221 cgcattctcc gcacgggtta atcatctggt agaagccctc acccacttgc gcatttacgt
    47281 cgaagcggca attgatttcc ctgatgaaga gatcgatttc ctctccgacg gcaaaattga
    47341 agcccagctt aatgacgtga tggccgatct cgatgcagtg cgtgctgaag cacgtcaggg
    47401 tagtttgttg cgcgaaggga tgaaagtagt gattgccgga cgtcctaatg ccggtaaatc
    47461 gagcctgtta aacgcgctgg cgggccgtga agcggcaatc gtaaccgata tcgccggaac
    47521 cacgcgtgac gtgctgcgtg agcatatcca cattgacgga atgccgctgc atatcatcga
    47581 cactgccggg ctacgtgagg ccagtgacga agtggaacgt attggtatcg agcgcgcgtg
    47641 gcaggaaatt gaacaggccg accgcgtgct gtttatggtc gatggcacca cgacagacgc
    47701 cgttgatccg gcagagatct ggccggaatt tatcgcccgt ctgcccgcga aactgccgat
    47761 caccgtggta cgcaataaag ccgatatcac cggcgaaacg ctgggaatga gtgaagtgaa
    47821 cggtcacgcg ttaattcgtc tctcggcaag gactggtgaa ggcgtggacg tgctgcgtaa
    47881 ccatctcaaa cagagcatgg gctttgacac caatatggaa ggcggcttcc tggcgcgccg
    47941 tcgccactta caggcgctgg aacaggcagc ggagcatttg cagcaaggta aagcgcaact
    48001 gttgggagcc tgggcaggtg aactgctggc ggaagagttg cgcctggcac agcagaactt
    48061 aagcgaaatc accggggaat ttacttcaga cgacctgctg gggcggattt tctccagctt
    48121 ctgtattggt aagtaaccgc gcttacgaag ccgtattctg actgtcagat gcggcttcgc
    48181 ttcattgtta gcactcctgt tatgcctcaa ccctttttta aacattaaaa ttcttacgta
    48241 atttataatc tttaaaaaaa gcatttaata ctgctccccg aacgattgtg attcgattca
    48301 catttaaaca atttcagaat agacaaaaat t
//
LOCUS       DAETQL010000024        47959 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.024, whole genome shotgun sequence.
ACCESSION   DAETQL010000024 DAETQL010000000
VERSION     DAETQL010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47959
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.024"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="K0W65_001464"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="K0W65_001464"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBI3094778.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="K0W65_001465"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="K0W65_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBI3094779.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="K0W65_001466"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="K0W65_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBI3094780.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="K0W65_001467"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="K0W65_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBI3094781.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="K0W65_001468"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="K0W65_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBI3094782.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="K0W65_001469"
     CDS             5377..5835
                     /locus_tag="K0W65_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBI3094783.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="K0W65_001470"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="K0W65_001470"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBI3094784.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="K0W65_001471"
     tRNA            6887..6971
                     /locus_tag="K0W65_001471"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="K0W65_001472"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="K0W65_001472"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="K0W65_001473"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="K0W65_001473"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBI3094785.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="K0W65_001474"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="K0W65_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBI3094786.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="K0W65_001475"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="K0W65_001475"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBI3094787.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="K0W65_001476"
     CDS             complement(10252..13995)
                     /locus_tag="K0W65_001476"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBI3094788.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="K0W65_001477"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="K0W65_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBI3094789.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="K0W65_001478"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="K0W65_001478"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBI3094790.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="K0W65_001479"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="K0W65_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBI3094791.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="K0W65_001480"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="K0W65_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBI3094792.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="K0W65_001481"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="K0W65_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBI3094793.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="K0W65_001482"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="K0W65_001482"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HBI3094794.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="K0W65_001483"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="K0W65_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBI3094795.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="K0W65_001484"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="K0W65_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBI3094796.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="K0W65_001485"
     CDS             23392..23499
                     /locus_tag="K0W65_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI3094797.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="K0W65_001486"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="K0W65_001486"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBI3094798.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="K0W65_001487"
     CDS             complement(24538..25347)
                     /locus_tag="K0W65_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HBI3094799.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="K0W65_001488"
     CDS             complement(25351..25743)
                     /locus_tag="K0W65_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBI3094800.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="K0W65_001489"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="K0W65_001489"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBI3094801.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="K0W65_001490"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="K0W65_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBI3094802.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="K0W65_001491"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="K0W65_001491"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBI3094803.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="K0W65_001492"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="K0W65_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBI3094804.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="K0W65_001493"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="K0W65_001493"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBI3094805.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="K0W65_001494"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="K0W65_001494"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBI3094806.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="K0W65_001495"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="K0W65_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBI3094807.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="K0W65_001496"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="K0W65_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBI3094808.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="K0W65_001497"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="K0W65_001497"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBI3094809.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="K0W65_001498"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="K0W65_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBI3094810.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36791..39658
                     /locus_tag="K0W65_001499"
     CDS             36791..39658
                     /locus_tag="K0W65_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBI3094811.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39758..41677)
                     /gene="dhaR"
                     /locus_tag="K0W65_001500"
     CDS             complement(39758..41677)
                     /gene="dhaR"
                     /locus_tag="K0W65_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HBI3094812.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41905..42975
                     /gene="dhaK"
                     /locus_tag="K0W65_001501"
     CDS             41905..42975
                     /gene="dhaK"
                     /locus_tag="K0W65_001501"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBI3094813.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42986..43618
                     /gene="dhaL"
                     /locus_tag="K0W65_001502"
     CDS             42986..43618
                     /gene="dhaL"
                     /locus_tag="K0W65_001502"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HBI3094814.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43629..45047
                     /gene="dhaM"
                     /locus_tag="K0W65_001503"
     CDS             43629..45047
                     /gene="dhaM"
                     /locus_tag="K0W65_001503"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HBI3094815.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45367..47064
                     /gene="treA"
                     /locus_tag="K0W65_001504"
     CDS             45367..47064
                     /gene="treA"
                     /locus_tag="K0W65_001504"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBI3094816.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47143..47582)
                     /locus_tag="K0W65_001505"
                     /pseudo
     CDS             complement(47143..47582)
                     /locus_tag="K0W65_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12042 a        12341 c        11745 g        11831 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccattgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgtttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggaag
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcattac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg ggttaaatat atattaattc ctgtgttcct gattttgccc ggtaatagag
    36661 aattattctc agagaagctc atcatgattg caccatacag atatcatgat gataagagga
    36721 tattattcat tgacttaaac aaaatgacaa aatccataga cccatagcct gggtactata
    36781 taggagtggt atgggcatca aacaacacaa tgggaatacc aaagccgatc gtctcgctga
    36841 attaaaaatc cgttcgccct caattcaact gataaaattt ggcgctattg gtttgaatgc
    36901 aattatcttt tcccccctgc tgatagctgc tgatacagga agtcaatatg gcaccaatat
    36961 tactattaat gatggtgaca gaattacagg agataccgcc gatccatcag gaaacctcta
    37021 tggtgtaatg accccagcag gaaacacgcc tggcaatatc aacctgggta atgatgtcac
    37081 cgtcaatgtc aacgacgcct ctggatatgc aaaaggaatc attattcagg gcaaaaacag
    37141 ctccctgaca gctaaccgac tcacagtaga tgttgttggt caaacctctg ccatcggcat
    37201 taacttaatt ggtgactata cccatgctga cttaggcaca ggcagcacca ttaagagtaa
    37261 cgatgacggc atcattattg ggcatagctc aacactaaca gccactcaat tcaccattga
    37321 aaactcgaac ggtataggcc taaccatcaa tgactatggc accagtgtcg atcttggaag
    37381 cggaagtaaa atcaagaccg atggaagtac aggtgtttat atcggtggtc tcaacggcaa
    37441 taacgccaat ggtgctgcgc gttttacggc gacagacctg acaatcgatg ttcagggcta
    37501 cagcgccatg gggataaacg tacagaaaaa ctctgttgtc gatctcggaa caaacagtac
    37561 cattaaaacc aatggcgata atgctcacgg cctctggagc tttggccagg ttagcgcgaa
    37621 tgcactcact gttgatgtaa ctggagccgc ggccaatggc gtcgaagttc gtggtggtac
    37681 aaccactatc ggtgcagata gccatatttc ttccgcgcag ggcggtggcc tcgtcaccag
    37741 tagttcagac gcgacaatca atttttctgg cacggcagcg caacgaaaca gcatcttttc
    37801 cggcggttct tatggtgcct cggcccagac ggcaacggct gttatcaaca tgcaaaatac
    37861 cgatattacg gttgatcgta atggcagtct ggcgctgggt ttgtgggcgc tcagcggcgg
    37921 tagaataacc ggagacagtt tggctatcac cggcgcggca ggagccagag ggatttatgc
    37981 catgaccaac agccagatcg acctcacgag cgatctggtc attgatatga gtacacccga
    38041 ccagatggcc atcgcaacgc aacatgacga tggttatgcc gccagccgca tcaacgcctc
    38101 gggtcgtatg cttatcaacg gtagcgttct ttccaaaggt gggctaatca atctggatat
    38161 gcaccctggg tcggtttgga caggttcctc cctcagcgat aatgtcaatg gcgggaaact
    38221 ggacgttgca atgaataaca gcgtctggaa cgtaacaagt aattctaatc tcgacacgct
    38281 ggcgctgagc cattcaactg tcgattttgc cagccacggg tcaactgccg gcacatttac
    38341 cacattaaac gtagagaacc tgagcggtaa cagtaccttt attatgcgtg ctgatgttgt
    38401 tggcgagggt aatggcgtta ataatagagg ggatttattg aatatcagcg ggagtagtgc
    38461 tggtaatcac gtattggcta tccgcaacca gggcagcgag gccacaacgg gaaatgaagt
    38521 tctgacagtg gtaaaaacca ctgacggcgc ggcctcgttc agcgcgtctt ctcaggttga
    38581 gttgggggga tatctgtacg atgtgcgtaa aaatggcact aactgggagc tttacgcttc
    38641 cgggacagtt ccggaaccga ctcctaatcc tgaacccaca ccagctcccg ctcagcctcc
    38701 catagtcaac cccgatccta cgcctgaacc cgctcccacg cctaaaccca ccacgaccgc
    38761 agatgctggc ggcaattatc tcaatgtcgg ttacttattg aactatgttg aaaaccgtac
    38821 gctgatgcaa cggatgggtg acttgcgaaa tcagagtaaa gacggtaata tctggttgcg
    38881 cagttatggg ggaagcctgg actcctttgc cagtggcaaa ctgagcggct ttgacatggg
    38941 ttacagcggt atccagtttg gtggggataa acgtctctct gatgtaatgc cgttgtatgt
    39001 cggtctgtat attgactcaa cacatgcatc gccggactat agcggaggcg acggtaccgc
    39061 acgttcagac tacatgggaa tgtacgccag ttacatggca caaaacggtt tttacagcga
    39121 tctcgttata aaagcatcgc gccagaaaaa tagtttccac gtactggaca gtcagaacaa
    39181 cggcgttaac gccaacggca ctgcgaatgg aatgagcatc tccctggaag ccgggcagag
    39241 gttcaacctg tcccctactg gttatgggtt ctatatagag ccgcaaaccc agcttacata
    39301 cagccaccag aatgagatgg ctatgaaggc gagtaatggc ctcaatatac atctgaatca
    39361 ctacgaatcg ctgctggggc gtgccagcat gatactgggg tatgacatca ccgcaggcaa
    39421 cagccagctg aatgtctatg tgaagactgg cgctatccgc gagttttcag gggataccga
    39481 atatctgttg aacgactccc gggagaagta cagtttcaaa ggtaatggct ggaataacgg
    39541 cgtgggagtc agtgcacagt ataacaaaca gcacacattc tatctcgaag cggattacac
    39601 gcagggtaac ctctttgatc agaagcaagt caacggagga tatcgcttca gcttttaagt
    39661 gcgggataga caagccctta aacgatgctg aaaattgtta gcgtgcgccg gaatgaaata
    39721 cagatcactc tgatgtatca gaaatcgttt ctgtctttca tacccggcgc ttaaactgcc
    39781 ctgcatcaat gccatgttgc ttcattttcc gccacagcgt agtgcggccg atcccaagta
    39841 aagccgacat ttcctgaatg cgaccgcctg tgacctgggc tgcgttaata attgcctctt
    39901 tttcaacttc cgcaaatgac agactggtgg aaaggcgggt ggcgctgaca tcatctgtcg
    39961 cctgctcggt aaacagatgt tcaggtaaat cactgacgcg aatgcgcccg ttatcactac
    40021 tcagagcaag attctcgatg acgctgtaaa gttcaaaatc gttgcctggc catgcacaag
    40081 aaactaggcg agcgagggca tcgtcatcaa ttttcagccg cgtagagaag cgtttttcaa
    40141 gactgcgtaa tttgttattc accagcgccg gaatgctgcc acgccgcata cgcagaggcg
    40201 ggatggtaat ttcaaatgca tgcagcgcgt aatacagctg gcgactaaaa cgattttgtt
    40261 ccaccagcat tgcgaggtcc gcggtcgttg ttgcaatcac tttgacatca attggtatta
    40321 aacgccgcgc atccagtcgc gtgataaccc cctgcttgat aacctgcagc aaagcagact
    40381 gtaactccac cgccagatat tcaatctttt caagaaacag cgtgccgccg tgtgccagtt
    40441 ccagccgact cagacggcca ttttcattgt ccgtgcgatc gccaccaata aattcttccg
    40501 ccagcgcagc atcaccatat aactcacaat tgacggcgat ataaggacct gcagcacgct
    40561 cgctttcatt atgaattgcc tgacttagca gtgccttgcc cacgccctct tctccacaaa
    40621 gcaggacagg aaagctactg cgcgccgcct ggcgaccaaa atgaatcaag cggcgggttt
    40681 gcggatcgtc ctgtggcata tgagcgaagg tatggctgac ttttcctaat tgactggtca
    40741 ttaactgccg catctgttcc acaggatgga gcaacaaaat aaagctggtt ccctgcgttt
    40801 ctattatcgg tttaagggtt atcaccgcat caataaactg gtgctggctt tcaaaggttg
    40861 cttctacgtg tttgagcgga tgtgccagtt ttattgcttg ttgcaatacg gcgggtaacg
    40921 ttaagagttc agtgattgcc cgtccctgac ttgccgtcgc gtcaaggcgc aagacccgcg
    40981 ccgcctgggc attaataaat tgcaaattac cctgctcgtc ccagctaatc acgccatcat
    41041 ccatactttc taacagggca ttaagttgat ttaaatgacg gttagtttca gcgagcaaac
    41101 tgtccgtcag cagtaaatta ccgacctcgc gggcgattgc caacgtcaac ggcaaatcag
    41161 ctgcggtagt ttgctcaacc ggacacgcca gcgctattgt tcccgtcaat cggcccttgc
    41221 tgtcaaacaa cggcgttgca caaaaggccc agttccagag tgcctgtttg aaatgttgat
    41281 cggccatcgt tttcacggcc tgaccagaga tagccgctaa cgatagcgca caagtaccaa
    41341 taattccctc ggcgcaatac gtgccgtcat tgaatcccag tgcacttagc tgctgcaagg
    41401 tttgcggatc gccattacgg ctgagaatgc aggcggtttc atcgaggata aacagcgcac
    41461 actctcgcgg tgccatatat tcccaggcat cttccagcgc agcctgcccg agcgtcagca
    41521 tcgctttctt acgccgataa atagaagcaa atgtcacgcc ctgggcctga tgtggtacgt
    41581 tccatgtctc ccgtttcatc agcttattgc atcgctccca ggaggttgca attaagggag
    41641 atatgccccg accatcgttg ttaaaagcgc cactcatatc ccgcatccct tatgacaaaa
    41701 ggtgttccgg ctcgcaattt taagtattaa tacacgtaaa agtttcaata tggaacattc
    41761 attaaagcat atgtttcact atgaaacata atttgggcta attttctttt cccttgcctg
    41821 atgcacaatg gattcatcgg cagtcagtgg tcgccgtgtc gttgaacatc atccatgccc
    41881 taccgtaatt gctggagcaa aataatgaaa aaattgatca atgatgtgca agacgtactg
    41941 gacgaacaac tggcaggact ggcgaaagcg catccatcgc tgacactgca tcaggatccg
    42001 gtgtatgtca cccgagctga tgcccctgtt gcaggaaaag tcgccctgct gtcgggtggc
    42061 ggcagcggac acgagccgat gcactgtggt tatatcggtc aggggatgct ttcgggggcc
    42121 tgtccgggcg aaattttcac ctcaccgacg cccgataaaa tctttgaatg ctccatgcaa
    42181 attgatggcg gcgaaggtgt actgttgatt atcaaaaatt acaccggcga tattcttaac
    42241 tttgaaacag cgaccgagtt actgcacgat agcggcgtaa aagtgaccac tgtggtcatt
    42301 gatgacgacg ttgcggtaaa agacagtctt tataccgccg ggcgacgcgg cgttgccaac
    42361 accgtattaa ttgaaaaact cgtaggcgca gcggcggagc gtggcgactc actggacgcc
    42421 tgtgcggaac tggggcgtaa gttgaataat caaggccact caataggtat cgctctcggt
    42481 gcctgtaccg ttcctgccgc gggcaaacct tcttttaccc ttgcggataa tgagatggag
    42541 tttggcgtag gcattcatgg tgagccaggt attgaccgcc gccctttctc ttcccttgat
    42601 caaaccgtcg atgaaatgtt cgacaccctg ctggaacatg gctcatacca tcgcactttg
    42661 cgtttctggg attatcaaca aggcagctgg caggaagaac cacaaaccaa acaaccgctc
    42721 cagtctggcg atcgggtgat tgcactggtt aacaatcttg gcgcaactcc gctttctgag
    42781 ctgtacggcg tctataaccg cctgaccaca cgttgccagc aagcgggatt gactatcgaa
    42841 cgtaatttaa ttggcgcgta ctgcacctca ctggatatga ccggtttctc aatcacctta
    42901 ctgaaagttg atgacgaaac gctggctctc tgggacgccc cggtccacac cccggccctt
    42961 aactggggta aataaggaga aagcaatgtc actgagcaga actcaaattg ttaactggct
    43021 cactcgttgt ggcgatattt tcaacaccga gagcgagtat cttaccgggc tggatcgcga
    43081 gattggcgac gctgaccacg ggctaaatat gaaccgaggc tttagcaaag tggtggaaaa
    43141 actccctgct atcgcagata aagatatcgg tttcattctc aaaaataccg gtatgacgct
    43201 gctttccagc gtcggtggtg ccagtggtcc gctgttcggt accttcttta tccgcgccgc
    43261 acaggcgacc caggcacggc aaagcctgac actggaagag ctttatcaga tgttccgcga
    43321 tggcgcggac ggcgtaatca gtcgcgggaa agccgaacct ggcgataaaa ccatgtgtga
    43381 tgtgtgggtg ccggtggtgg aatcgttacg tcagtccagc gagcaaaatc tctctgttcc
    43441 ggtggcgctc gaagctgcca gtagcatcgc cgaatccgct gcacaaagta cgattacgat
    43501 gcaagcccgc aaaggccgcg ccagttatct cggtgaacgc agtattggtc accaggatcc
    43561 cggcgcgacc tcggtgatgt ttatgatgca aatgttggcg ttagccgcaa aagagtaagg
    43621 aattggtgat ggtaaacctg gtcatagttt cacatagcag ccgactggga gaaggtgtcg
    43681 gtgaattagc ccgtcagatg ttaatgagtg atagttgtaa aatcgccatt gccgcgggaa
    43741 ttgacgatcc acaaaatccc attggtaccg atgccgtcaa agtgatggag gccatcgaat
    43801 ctgttgctga tgccgaccat gtgctggtca tgatggatat gggtagcgca ttattgagtg
    43861 ctgaaactgc gctggaattg ctggctcccg agatcgccgc aaaagtacgt ttgtgtgctg
    43921 cgccgttggt cgaaggtaca ctggcagcaa cggtcagcgc ggcctcgggg gcggatatcg
    43981 acaaagttat ctttgacgct atgcatgcgc tggaagccaa acgtgaacaa ctgggtttac
    44041 cgtcctccga cactgaaatc tctgacacat gtcctccgta cgatgaagaa gcccgttctc
    44101 tggcggtggt cataaaaaac cgtaacggcc tgcatgtacg tccggcctcc cggctggttt
    44161 ataccttatc gacatttaat gccgatatgt tgctggaaaa aaacggcaaa tgcgtcacac
    44221 cagagagtat taaccagatt gcgttactac aagttcgcta taacgatacg ctgcgcctga
    44281 ttgcgaaagg gccagaagct gaagaggcac tgatcgcttt ccgtcacctg gctgaagata
    44341 actttggtga aacggaggaa gtcgctccac ctactctgcg tcccgttctg cctgtttcgg
    44401 gtaaagcctt ttattatcaa ccagttttat gtacggtaca ggcaaaatca accctgaccg
    44461 cggaagaaga acaagatcga ttacgccagg ctattgactt cacgttatta gatctgatga
    44521 cgttaacagc gaaagcagaa gccagcgggc ttgacgatat tgccgcaatc ttttctggtc
    44581 accatacact gttaggtgat ccggaactgc tggcggcggc aagcgaactc cttcagcatg
    44641 aacattgcac ggcagaatat gcctggcagc aagttcttaa agaacttagc cagcaatacc
    44701 agcaactgga tgatgaatat ctacaagctc gctatattga tgtggacgat cttctgcatc
    44761 gcaccctggt ccacctgacc caaacgaaag aagaactccc gcagtttaac tcgccaacta
    44821 ttctactggc ggagaacatt tatccttcca cagtactgca actggatccg gcggttgtaa
    44881 aaggtatctg ccttagcgcc ggaagtccgg tatcccacag cgccctaatc gcccgtgaac
    44941 tggggattgg ctggatttgc cagcagggtg agaaactgta tgcgatacaa ccagaagaaa
    45001 cgctaacgct ggacgttaaa acgcaacgtt tcaaccgtca gggttaagcc accgttcgga
    45061 tggcatcgtt ctgatgtcat ccggcaattt acccagcact gatgtttttt aactacctgt
    45121 tatatttttt accaatttat gatgattttt cttcgctaat cgcgaaccaa atgtcgctgg
    45181 ctggctaata aacagacgtt atttgatcac tctatttcgc cgttatttat ccctttaatt
    45241 cccttttcta aaatgcctga ccgttcgcag aatgagattt cgatcatgca gctagtgcga
    45301 tcctgaacta aggttttctg atacttgaat accgttttat tcggtttcgc caaaggagaa
    45361 tgattgatga aatcccccgc accttctcgc ccgcaaaaaa tggcgttaat tccagcctgt
    45421 atctttttgt gtttcgctgc gctatcggtg caggcagaag aaacaccggt aacaccacag
    45481 ccgcctgata ttttattagg gccgctgttt aatgatgtgc aaaacgtcaa actttttccg
    45541 gaccaaaaaa cctttgccga tgccgtgcct aacagcgatc cgctgatgat ccttgctgat
    45601 tatcggatgc agcaaaacca gagcggattt gatctgcgcc atttcgttaa cgtcaatttc
    45661 accctgccga aagaaggcga gaaatatgtt ccgccagagg ggcagtcact gcgcgaacat
    45721 attgacggac tttggccggt attaacgcgt tctaccgaaa acaccgaaaa atgggattct
    45781 ctgttaccgc tgccggaacc ttatgtcgtg ccgggcggac gttttcgcga ggtatattac
    45841 tgggacaatt acttcaccat gttaggactt gccgaaagtg gtcactggga taaaatcgcg
    45901 gatatggtgg ccaattttgc tcatgaaata aacacttacg gtcatattcc caacggcaac
    45961 cgcagttact atttaagccg ctcgcaaccg cccttctttg ccctgatggt agagttactg
    46021 gcgcagcatg aaggcgatgc cgcgttgaag caatacctgc cgcaaatgca aaaagaatat
    46081 gcttactgga tggacggtgt tgaaaacctg caagccggac aacaggaaaa acgcgttgta
    46141 aaacttcagg atagtaccct tctcaaccgc tactgggacg atcgcgatac gccacgacca
    46201 gagtcatggg tggaagatat tgccaccgcc aaaagcaatc cgaatcgacc tgccactgaa
    46261 atttaccgcg acctgcgctc tgccgctgcg tctggctggg atttcagctc gcgctggatg
    46321 gacaacccgc agcagttaaa taccttacgc accaccagca tcgtaccggt cgatctgaac
    46381 agcctgatgt ttaaaatgga aaaaatcctc gcccgcgcca gcaaagctgc aggagataac
    46441 gcgatggcaa accagtacga aacgctggcg aatgcccgtc aaaaagggat cgaaaaatac
    46501 ctgtggaacg atcaacaagg ctggtatgcc gattacgacc tgaaaagtca taaagtgcgc
    46561 aatcagttaa ccgcggccgc cctgttcccg ctgtacgtca atgcggcagc gaaagatcgc
    46621 gccaacaaaa tggcgacggc gacgaaaaca catctgctgc aacccggcgg cctgaacacc
    46681 acgtcggtga aaagtgggca acaatgggat gcgccaaatg gctgggcacc gttacagtgg
    46741 gtcgcgacag aaggattaca aaactacggg caaaaagagg tggcgatgga cattagctgg
    46801 cacttcctga ccaatgttca gcacacctat gaccgggaga aaaagctggt ggaaaaatat
    46861 gatgtcagca ccaccggaac ggggggcggc ggtggcgaat atccattaca ggatggcttt
    46921 ggctggacca atggcgtgac gctgaaaatg ctggatttga tctgcccgaa agagcaaccg
    46981 tgtgacaatg ttccggcgac gcatccgacc gttaagtcag caacgacgca accctcaacc
    47041 aaagaggcac aacccacacc ttaaccagcg cttactccga caacgtcatt cggcttctcc
    47101 ggatggcgtt gtcggcagat aacaccatat ctatggtttt ttctacacag acggtttttc
    47161 aagtcctggc gggaagtatt gcgcaaatgt ttgccatacg tggttgccga agatagcgtc
    47221 atttctacgg gtaagtgttt cactttcatc acccggcgat ttacgccgac gttggctata
    47281 taaagcatca attgctaccg aatattgtta cggtgttcac tcatcaacgt ttttttgctt
    47341 tctggagtta aattaataat cacataatcc agacactcca gagaatttag caaccaaaaa
    47401 aagaattcag accattcctc ttcgcgtaac tgaagcattg ccgaacgggt attttggctt
    47461 aatctgccag aaggattgat caattctaaa tgttcaataa gcgcagccaa aaccgcctca
    47521 ctatccgccc catttttaac gcctgactgg ctactgtcta aaatatttat tttctgaatc
    47581 acaccaaaaa caacgaaaca ataattaaaa aaggattctg ttgttgttac cgggaaccga
    47641 cttatttcgc tcagaaagtc gttcaaccag ctccgcagtg ataagattcg ctgatgatac
    47701 agtgtatcct cactgcgtgc tggaggtgcc agtataatat gttgatcaat acagtattta
    47761 ttgtgttcag tcgtttcctg atgcgtttgg ttctctgcgg attgtcgctg gcgaaataaa
    47821 tggcttgcgg taaatgccgt tacagagcag cgcaacgcgt caattccttc ggagtgcccg
    47881 acttttggcg agagttccgg ttttatgcgt ccaggtagcg gaaatttcac ccgaatgaga
    47941 aatgcattaa taaaatgga
//
LOCUS       DAETQL010000025        47798 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.025, whole genome shotgun sequence.
ACCESSION   DAETQL010000025 DAETQL010000000
VERSION     DAETQL010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.025"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="K0W65_001506"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="K0W65_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBI3094817.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="K0W65_001507"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="K0W65_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBI3094818.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="K0W65_001508"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="K0W65_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="K0W65_001509"
     CDS             1631..2341
                     /locus_tag="K0W65_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBI3094819.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="K0W65_001510"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="K0W65_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBI3094820.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4119)
                     /locus_tag="K0W65_001511"
     CDS             complement(3469..4119)
                     /locus_tag="K0W65_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBI3094821.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4282..6615
                     /gene="bisC"
                     /locus_tag="K0W65_001512"
     CDS             4282..6615
                     /gene="bisC"
                     /locus_tag="K0W65_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HBI3094822.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6584..7024)
                     /locus_tag="K0W65_001513"
     CDS             complement(6584..7024)
                     /locus_tag="K0W65_001513"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBI3094823.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7021..7584)
                     /gene="tag"
                     /locus_tag="K0W65_001514"
     CDS             complement(7021..7584)
                     /gene="tag"
                     /locus_tag="K0W65_001514"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBI3094824.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7742..8440
                     /locus_tag="K0W65_001515"
     CDS             7742..8440
                     /locus_tag="K0W65_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBI3094825.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8669..9877
                     /locus_tag="K0W65_001516"
     CDS             8669..9877
                     /locus_tag="K0W65_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3094826.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10201..11892
                     /gene="eptB"
                     /locus_tag="K0W65_001517"
     CDS             10201..11892
                     /gene="eptB"
                     /locus_tag="K0W65_001517"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBI3094827.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11984..12060
                     /locus_tag="K0W65_001518"
     tRNA            11984..12060
                     /locus_tag="K0W65_001518"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12018..12020,aa:Pro,seq:cgg)
     gene            12804..14411
                     /gene="dppA"
                     /locus_tag="K0W65_001519"
     CDS             12804..14411
                     /gene="dppA"
                     /locus_tag="K0W65_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBI3094828.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14719..15738
                     /gene="dppB"
                     /locus_tag="K0W65_001520"
     CDS             14719..15738
                     /gene="dppB"
                     /locus_tag="K0W65_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBI3094829.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15748..16650
                     /gene="dppC"
                     /locus_tag="K0W65_001521"
     CDS             15748..16650
                     /gene="dppC"
                     /locus_tag="K0W65_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBI3094830.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16661..17644
                     /gene="dppD"
                     /locus_tag="K0W65_001522"
     CDS             16661..17644
                     /gene="dppD"
                     /locus_tag="K0W65_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBI3094831.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17641..18645
                     /gene="dppF"
                     /locus_tag="K0W65_001523"
     CDS             17641..18645
                     /gene="dppF"
                     /locus_tag="K0W65_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBI3094832.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18675..19946)
                     /locus_tag="K0W65_001524"
     CDS             complement(18675..19946)
                     /locus_tag="K0W65_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBI3094833.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20422..20529
                     /locus_tag="K0W65_001525"
     CDS             20422..20529
                     /locus_tag="K0W65_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI3094834.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            20905..21012
                     /gene="ldrD"
                     /locus_tag="K0W65_001526"
     CDS             20905..21012
                     /gene="ldrD"
                     /locus_tag="K0W65_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HBI3094835.1"
                     /translation="MTFAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21388..21495
                     /locus_tag="K0W65_001527"
     CDS             21388..21495
                     /locus_tag="K0W65_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI3094836.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            21871..21978
                     /locus_tag="K0W65_001528"
     CDS             21871..21978
                     /locus_tag="K0W65_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBI3094837.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(22065..23744)
                     /gene="bcsG"
                     /locus_tag="K0W65_001529"
     CDS             complement(22065..23744)
                     /gene="bcsG"
                     /locus_tag="K0W65_001529"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBI3094838.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(23741..23932)
                     /gene="bcsF"
                     /locus_tag="K0W65_001530"
     CDS             complement(23741..23932)
                     /gene="bcsF"
                     /locus_tag="K0W65_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBI3094839.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(23929..25500)
                     /gene="bcsE"
                     /locus_tag="K0W65_001531"
     CDS             complement(23929..25500)
                     /gene="bcsE"
                     /locus_tag="K0W65_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBI3094840.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            25773..25961
                     /locus_tag="K0W65_001532"
     CDS             25773..25961
                     /locus_tag="K0W65_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HBI3094841.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            25973..26725
                     /gene="bcsQ"
                     /locus_tag="K0W65_001533"
     CDS             25973..26725
                     /gene="bcsQ"
                     /locus_tag="K0W65_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HBI3094842.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            26722..27081
                     /locus_tag="K0W65_001534"
     CDS             26722..27081
                     /locus_tag="K0W65_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HBI3094843.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            27078..29339
                     /gene="bcsA"
                     /locus_tag="K0W65_001535"
                     /pseudo
     CDS             27078..29339
                     /gene="bcsA"
                     /locus_tag="K0W65_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            29350..31689
                     /gene="bcsB"
                     /locus_tag="K0W65_001536"
     CDS             29350..31689
                     /gene="bcsB"
                     /locus_tag="K0W65_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBI3094844.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            31696..32802
                     /gene="bcsZ"
                     /locus_tag="K0W65_001537"
     CDS             31696..32802
                     /gene="bcsZ"
                     /locus_tag="K0W65_001537"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBI3094845.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            32879..33220
                     /locus_tag="K0W65_001538"
     CDS             32879..33220
                     /locus_tag="K0W65_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HBI3094846.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLWLHGAGIT"
     gene            33135..33386
                     /locus_tag="K0W65_001539"
                     /pseudo
     CDS             33135..33386
                     /locus_tag="K0W65_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            33468..35456
                     /gene="hmsP"
                     /locus_tag="K0W65_001540"
     CDS             33468..35456
                     /gene="hmsP"
                     /locus_tag="K0W65_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HBI3094847.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            35644..36930
                     /gene="dctA"
                     /locus_tag="K0W65_001541"
     CDS             35644..36930
                     /gene="dctA"
                     /locus_tag="K0W65_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HBI3094848.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            37151..38647
                     /locus_tag="K0W65_001542"
     CDS             37151..38647
                     /locus_tag="K0W65_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBI3094849.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(38743..39672)
                     /gene="kdgK"
                     /locus_tag="K0W65_001543"
     CDS             complement(38743..39672)
                     /gene="kdgK"
                     /locus_tag="K0W65_001543"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HBI3094850.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            39904..40671
                     /gene="pdeH"
                     /locus_tag="K0W65_001544"
     CDS             39904..40671
                     /gene="pdeH"
                     /locus_tag="K0W65_001544"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBI3094851.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            40741..42801
                     /locus_tag="K0W65_001545"
     CDS             40741..42801
                     /locus_tag="K0W65_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBI3094852.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(42983..44305)
                     /locus_tag="K0W65_001546"
     CDS             complement(42983..44305)
                     /locus_tag="K0W65_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBI3094853.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(44716..45729)
                     /gene="yhjD"
                     /locus_tag="K0W65_001547"
     CDS             complement(44716..45729)
                     /gene="yhjD"
                     /locus_tag="K0W65_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBI3094854.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(45778..46677)
                     /locus_tag="K0W65_001548"
     CDS             complement(45778..46677)
                     /locus_tag="K0W65_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3094855.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            47197..>47798
                     /locus_tag="K0W65_001549"
     CDS             47197..>47798
                     /locus_tag="K0W65_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBI3094856.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT        11445 a        11910 c        12858 g        11585 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggatctt
     4561 attcaccaac aacataaacg cattcgtgag gcttatggtc cggcatcgat ttttgctggt
     4621 tcctacggct ggcgttcaaa cggcgtgctg cataaggcct cgacattatt acaacgctat
     4681 atggcgctgg caggcggtta taccgggcat ctgggggatt attcgaccgg tgcggcacag
     4741 gcgatcatgc cgtatgtcgt aggtggcagc gaagtttatc aacagcaaac cagttggccg
     4801 ctggtgctgg aacatagcga tgtcgtggtg ttgtggagtg ctaacccact caatacgctg
     4861 aaaattgcgt ggaatgcatc cgatgagcag gggctttctt acttttccgc actgcgtgac
     4921 agtgggaaaa agctgatctg cattgatcca atgcgatcgg aaaccgtcga tttctttggc
     4981 gataaaatgg agtgggtggc accgcacatg ggcaccgatg ttgcgctgat gctggggatc
     5041 gcccatacgc tggtggaaaa tggttggcac gacgaagcgt ttctggcgcg ttgcaccaca
     5101 ggttatgccg tcttcgcctc ttatttgctg ggcgagagtg acggaatagc gaaaaacgcc
     5161 gaatgggcgg cagagatttg tggtgttggc gcagcgaaaa tccgcgagct ggcggctatt
     5221 ttccaccaaa ataccaccat gctgatggca ggctggggaa tgcagcgcca acagtttggt
     5281 gagcaaaaac actggatgat cgtcacgctg gcagcaatgt tggggcaaat cggcacaccc
     5341 ggcggcggtt ttggtctttc ttaccatttt gccaatggtg gtaaccccac gcggcgttct
     5401 gcggtgctct cttccatgca gggcagcttg ccgggtggct gcgatgcggt ggataaaatc
     5461 cctgttgccc gcattgttga agcactggaa aaccctggtg gcgcatatca acacaacggt
     5521 atgaaccgac atttcccgga tattcgtttt atctggtggg cgggcggtgc caactttact
     5581 catcatcagg ataccaatcg tctgatccgt gcctggcaaa aaccggagct ggtggtgatc
     5641 tctgaatgct tctggacggc ggcggcgaaa cacgcggata ttgttctgcc tgcgactacc
     5701 tcttttgagc gtaatgatct caccatgacc ggtgattaca gtaatcagca tctggtgccg
     5761 atgaagcaag tggtgccgcc acgctatgaa gcgcgtaatg attttgatgt ttttgccgag
     5821 ttgagtgaac gttgggagaa gggcggctat gcgcggttta cggaaggaaa aagtcagctg
     5881 caatggctgg aaacgtttta taacgttgcc cggcagcgcg gggcaagcca gcaggttgaa
     5941 ttgccgccat ttgctgagtt ctggcaagcc aaccagttaa ttgagatgcc ggaaaacccg
     6001 gacagcgagc ggtttattcg cttcgctgat ttttgccgcg atccgctggc gcatccgtta
     6061 aaaaccgcca gcggcaagat tgaaatcttc tcacagcgta ttgccgatta cggttacccg
     6121 gattgccctg ggcatccaat gtggctggag ccggacgaat ggcagggcaa tgccgaacca
     6181 gaacagttgc aggtactttc tgcccatccg gcgcaccgcc tgcacagcca gctgaattac
     6241 agttctctgc gcgaattgta cgcggtagca aaccgtgagc ctgtcaccat tcatcctgac
     6301 gatgctcagg cgcgcggcat aaccgaaggg gatatggtgc gggtgtggaa ctcccgtggg
     6361 cagatccttg ccggagcggt cattagcgag ggaattaaac ctggcgtgat ttgcattcac
     6421 gaaggggcat ggccggatct ggatttaacc gctgacggta tttgtaaaaa cggcgcagtg
     6481 aacgtgctga ccaaagatct ccccagctcg cggctgggga atggctgtgc gggtaatacg
     6541 acgctggcat ggctggaaaa atacaacggt ccggaactga cacttacagc gtttgaacca
     6601 ccggccagct cataatccag gtgggtagtt gggtttcatc ctgccatgcg caatcgacaa
     6661 tgtgaaaacc ctgcgcacgg taaaaatcta tcgccggttg atttttttga taaacctcca
     6721 gcatcagatg gggatagcgc tgctgcacat actgcatcag cgccttacca ataccgcgtc
     6781 tgacggcctt cggtgcgaca aacattgctg ccagaaatcg gccttccata atgctgacaa
     6841 aaccgagaag cttaccgtcc tcttcccaga cccagttttg cgcgttggca agataggcat
     6901 cccgcaccag cggaatgcag tcacgccagt aattcgcttt tataaaggga tgcccccagg
     6961 ttgtactttc cagccacagt tcgaggatcg cggggagttc tgaacgttgc gcttcccgaa
     7021 tcatggttta tttcccagat agcaacagca gccaaccaca tgatcattca ccagcccaca
     7081 tgcctgcata aaggagtaac agattgtggt gccgacaaac ttaaaaccac gttttttcag
     7141 tgccttagat agggcgtcgg aggcggacgt agatgtggga atttcgctca acgttgtggc
     7201 ttgtgtcacc tgtggctgat gatttacaaa cgaccagaca aagtcgggaa acggttcgcc
     7261 gttctgttcc atttgcaggt acgcccgcgc attaccaata attgcctgaa ttttccctcg
     7321 atggcggata atcccggcgt cctgtaccag tctttcgaca tcctcttcct gcattgctgc
     7381 gaccttcacc ggatcgaact gatgaaagta ggcgcgatag ttttcgcgtt ttttgaggac
     7441 ggtgatccac gataacccag cctgctgccc ttcaaagcag atcatttcga acagtttttt
     7501 actgtcagtt tcaggcacgc cccactcatt atcatggtag gcaatataaa gcgggccctg
     7561 actcacccag ccgcaacgtt ccatactttc cctcgctatg tacccgatcg taaaaatttc
     7621 agccgctttt cttgacgcat cggtgaaaaa gtcaatattt aatacagggc tactatgccc
     7681 gatatccttc atacaatgac aataatattg ccgaattcgg ctcttccctg gagtcgtgtt
     7741 aatgatcata aaaaaaagcg gtggtcgctg gcagctaagc ctgctggcga gcgtggtaat
     7801 cagtgccttt tttctcaaca cagcttacgc ctggcaacaa gaatatatcg ttgatacgca
     7861 acccggacat tccacagagc gttacacctg ggatagtgat catcaacctg attacaacga
     7921 tattttgtcg caacgtattc aaagtagcca aagggcgctg ggactggaag tcaatctggc
     7981 ggaagaaact cctgtggatg tgaccagcag tatgagtatg ggctggaatt ttcctttata
     8041 tgaacaggtt acaaccggcc cggtcgcggc attacattac gatggcacaa ccacctcgat
     8101 gtataacgag tttggcgaca gtactaccac gctgaccgat ccgttatggc atgccagcgt
     8161 gagtagctta ggctggcgtg ttgactcccg gcttggcgat ctccgaccct gggcgcaaat
     8221 cagctataac cagcaatttg gcgagaatat ctggaaggcg caatcaggcc tgagccggat
     8281 gacggcgaca aaccagaacg gcaactggct ggatgtcacc gtaggcgctg atatgttgct
     8341 caatcaaaat attgccgcct atgctgcgct aactcaggca gaaaatacca ctaataatag
     8401 cgactatctg tatacgatgg gggttagcgc cagattttaa cgtaacagtc acaattgaaa
     8461 ccattgaata acaacagttg tggcgatagt gggtgctaac ttaccaaata ataaatttgg
     8521 tgaataattg tcgcgttatt cattcctgaa ctaaggcatt tcattccgtt ctgatggcat
     8581 ttcatgccgt ttttccccaa gcataaaatg cacttcgtta tggttgtcgg cagagatttt
     8641 tcctttttat tactgcagga atactgccat gacaccttca aattatcagc gtacccgctg
     8701 gctgacactc atcggtacta tcattaccca gtttgcgctg gggtcggttt atacccggag
     8761 cctgtttaat ggcgcgcttt ctgccaagct ggatgcgccg gtaagccagg tcgctttctc
     8821 tttcggcttg ttaagtctgg ggctggcaat ttcgtcttct gttgcgggca aattgcagga
     8881 acgttttggc gttaaacgcg tcaccgtggc ttccggcatt ttgctgggat taggcttctt
     8941 cctgaccgcg cattccaaca acctgatgat gctgtggtta agcgccggtg tgctggtcgg
     9001 tctggcggat ggcgcgggtt acctgctgac gctctctaac tgcgtgaagt ggttcccgga
     9061 gcgtaaaggt ctgatctccg cgttcgctat cggttcttat ggtctgggta gcctgggttt
     9121 caaatttatc gacacgcagc tgctcgaaac ggtgggtctg gaaaaaacct ttgtgatttg
     9181 gggagcgatt gcgctggtga tgattgtttt cggcgcaacg ttaatgaaag acgcaccaaa
     9241 acaggaagtg aaaaccagca atggtgtggt ggagaaagat tacacgctgg cagagtcgat
     9301 gcgtaaaccg cagtactgga tgttagcggt aatgttcctg actgcctgca tgagcggcct
     9361 gtacgtgatt ggggtagcga aagatatcgc ccaaagtctg gcacaccttg atgtggtttc
     9421 cgcagccaat gcagtaacgg ttatttccat cgctaacctt tcaggtcgtc tggtgctggg
     9481 cattctgtct gacaaaattg cccgtatccg tgttatcacc attggtcagg tgatttcgct
     9541 ggtgggtatg gcggccctgc tgtttgcacc attaaatgca gtgacgttct ttgcagcgat
     9601 tgcctgcgtg gcatttaact ttggcggcac tattaccgtc tttccatcac tggtcagtga
     9661 gttcttcggc ctcaataacc tggcgaaaaa ctacggtgtg atttacctcg gtttcggtat
     9721 cggtagcatt tttggttcga ttatcgcctc actgtttggc ggtttctatg tgaccttcta
     9781 cgtgattttc gccctgctga ttctgtcatt ggcgctttct acgacgattc gtcagccaga
     9841 gcagaaaatg ttgcgtgagg cgcatggctc cctttaatgc tcagccgcga gctactgggc
     9901 ttcagcttcc gtgtgcatca aacgcatcaa cgaaatgtat aaacaatgac tcgtaaaatt
     9961 tgggccagtt aactggccct ttttactgct ttttgtaacg ctaaagtctc ttttcaaact
    10021 tgcatttttg taaatttgtg cttcatgcac actctttccc cacacttttt cgctttgctg
    10081 tggtctactt attcgcgcgt gtagatttta cttatctgac tacctccgca cttttcccct
    10141 gccgggcctg aaaagccgct aagcagggtg ttatcacctg tttgtccagg gtttgtttgc
    10201 atgagataca tcaaatcgat tacacagcag aagctgagct ttttgcttgc aatctatatt
    10261 ggccttttta tgaatggcgc ggttttttat cgccgcttcg gcagctatgc gcacgatttt
    10321 accgtctgga aaggcgtttc tgctgttgtt gaactggccg ccaccgtact ggtgaccttc
    10381 tttttactac gtcttctatc gctgtttggc cgccgcagct ggcgtattct ggcgtcgctg
    10441 gtggtgctct tttccgcagg tgccagctat tacatgacct tccttaatgt ggtcattggt
    10501 tatggcatca tcgcttccgt catgaccacc gatatcgacc tgtcaaaaga agttgttggt
    10561 ctgaacttta ttctctggtt aatcgccgtt agtgcattgc ctcttatcct tatctggaat
    10621 aaccgctgtc gctacacctt gctccgacaa ctgcgaaccc cagggcagcg tattcgcagc
    10681 ctggtggtcg tcgtactggc gggtattatg gtttgggcac cgattcgttt gctggatatc
    10741 cagcagaaga aagtggagag ggcgaccggc gttgatttgc cgagttatgg cggtgtcgta
    10801 gcgaactctt atctgccatc aaactggctt tctgcgttgg ggctgtatgc ctgggcgcgg
    10861 gtcgatgaat cttccgataa taattcattg cttaatccgg cgaagaagtt tacataccag
    10921 gctccacaaa acgttgatga tacttatgtc gtgtttatca tcggtgaaac cacgcgttgg
    10981 gaccatatgg gtattttcgg ctatgagcgt aataccacgc cgaaactggc ccaggagaaa
    11041 aatctggcgg cgttccgtgg ttactcctgt gataccgcaa ccaaactctc actgcgttgc
    11101 atgtttgtac gtcagggggg cgcggaagat aatccgcagc gcacattaaa agaacagaac
    11161 attttcgcgg ttctgaagca gttaggattc agttctgacc tctacgctat gcagagcgaa
    11221 atgtggttct acagcaacac gatggcagac aacattgctt atcgtgagca gattggtgcg
    11281 gagccacgta atcgtggcaa gccggtagat gatatgttgc tggtagacga aatgcagcaa
    11341 tcgctagggc gcaacccgga tggtaagcat ctgatcattc tgcataccaa aggttcgcat
    11401 tttaactaca cccagcgtta tccgcgtagc ttcgcgcagt ggaagccgga atgtattggt
    11461 gttgatagcg gctgtaccaa agcgcagatg atcaactcct atgacaactc ggtgacctat
    11521 gtggatcact ttatctccag cgtgattgat caggttcgcg ataagaaagc gattgtgttc
    11581 tacgcagctg accacggtga gtcaattaat gaacgcgagc acctgcacgg cacgccgcgt
    11641 gaactggcac cgccggagca gttccgcgta ccgatgatgg tctggatgtc agataaatat
    11701 ctggaaaatc cggccaatgc gcaggcgttt gcgcagctga aaaaagaagc cgacatgaaa
    11761 gtgccacgcc gtcacgtaga gctgtacgac accatcatgg gttgtcttgg ctatacttca
    11821 ccggatggtg gaattaacga aaacaacaac tggtgtcaca tcccgcagac aaaagtggca
    11881 gcggctaact aagcggcctg ctgactttct cgccgatcaa aaggcatttt gctattaagg
    11941 gattgacgag ggcgtatctg cgcagtaaga tgcgccccgc attcggtgat tggcgcagcc
    12001 tggtagcgca cttcgttcgg gacgaagggg tcggaggttc gaatcctcta tcaccgacca
    12061 aattcgaaaa gcctgctcaa cgagcaggtt tttttgcatt tgtagctcat gaggatgaga
    12121 acctccgggg gcagggaggc tcgactcgac gtaggcctga taagacgcgc cagcgtcgca
    12181 tcaggcaacg gcaccgcact tattgtcgga tgcggcgcga ccgccttatt cgaccaacac
    12241 accacacatt ttcctgccaa ctttgcgccc catatctcac tatccattaa cggatttgtg
    12301 acagaaacca ttgtcttttt ttatatttgc ttaaatcttt tttgcgttac ttatggctgg
    12361 cgctggcatt ttccgctgtg cgttttagcg ttcatggtat taatcgctca gatatccacc
    12421 ttttcctcag atttttttca ttaaatgcat aaaacttaat caccaaatgt tgctaaatat
    12481 taagctgatt gttctggtta tgttggagta gcacaaattt ctctgctgca aatagctgtt
    12541 tgaagttttt ttaatctttg tttgtggatt ctcaccgttg gtgtaaatcc cggttggctg
    12601 tattgacgtt ttcacattct gttgacagat tgtaggtcac gaggggcatt ttatggagga
    12661 tccgcactgt tacactgatg ttaattagta cggcatcccc acctcataac gttgacccga
    12721 ccgggcaaaa aacaaaaaag gtcaggcagc gacaacccac tgcaaagggt taaaacaaca
    12781 aacatcacaa ttggagcaga ataatgcgta tttccttgaa aaagtcaggg atgctgaagc
    12841 ttggtctcag cctggtggct atgaccgtcg cagcaagtgt tcaggctaaa actctggttt
    12901 attgctcaga aggatctccg gaagggttta acccgcagct gtttacctcc ggcaccacct
    12961 atgacgcctc ttccgtcccg ctttataacc gcctggttga atttaaaatc ggcaccaccg
    13021 aagtgatccc gggcctcgct gaaaagtggg aagtcagcga agacggtaaa acctatacct
    13081 tccatctgcg taaaggtgtg aagtggcacg acaataaaga attcaaaccg acgcgcgaaa
    13141 tgaacgccga tgacgtggtg ttctcgttcg atcgtcagaa aaacgcgcaa aacccgtacc
    13201 ataaagtttc tggcggcagc tacgaatact tcgaaggtat gggcttgccg gagctgatca
    13261 gcgaagtgaa aaaggtggac gataacaccg ttcagtttgt gctgactcga ccggaagcgc
    13321 cgttcctcgc tgacctggca atggacttcg cctctattct gtcaaaagaa tatgctgacg
    13381 cgatgatgaa agccggtaca ccggaaaaac tggacctcaa cccaatcgga accggtccgt
    13441 tccagttaca gcagtaccaa aaagattccc gtatccgcta caaagcgttt gatggctact
    13501 ggggcaccaa accgcagatc gatacactgg tcttctctat tacccctgat gcttccgtgc
    13561 gttacgcgaa attgcagaag aatgaatgcc aggtgatgcc gtacccgaac ccggcagaca
    13621 tcgctcgcat gaagcaggat aaatccatca acctgatgga aatgccgggg ctgaacgtcg
    13681 gctacctctc gtataacgtg cagaaaaaac cgctggatga cgtgaaagtt cgccaagcac
    13741 taacctacgc ggtgaacaaa gacgcgatca tcaaagcggt ttatcagggc gcgggtgtat
    13801 cagcgaaaaa cctgatcccg ccaaccatgt ggggctataa cgacgacgtt caggattaca
    13861 cctacgatcc tgaaaaagcg aaagccttgc tgaaagaagc gggtctggaa aaaggtttct
    13921 ccatcgacct gtgggcgatg ccggtacaac gtccgtataa cccgaacgct cgccgcatgg
    13981 cggagatgat tcaggcagac tgggcgaaag tgggcgtgca ggccaaaatc gtcacctacg
    14041 aatggggtga gtacctcaag cgtgcgaaag atggcgaaca tcagacggta atgatgggct
    14101 ggactggcga taacggggac ccggataact tcttcgccac cctgttcagc tgcgccgcct
    14161 ctgaacaagg ttctaactac tcaaaatggt gctacaaacc gtttgaagat ctgattcaac
    14221 cggcgcgtgc taccgacgac cataacaaac gcgttgaact gtacaagcaa gcgcaggtgg
    14281 tgatgcacga tcaggctccg gcactgatca tcgctcactc caccgtgttt gaaccggtac
    14341 gcaaagaagt caaaggctat gtggttgatc cattaggcaa acatcacttc gaaaacgtct
    14401 ctatcgaata attaaaagcc atacaagact gatggcaaag gcaaaactgc ctgatgtgct
    14461 ccgcttatca ggcctacgaa aattctgcaa tgtattgaat ttgcacgatt ttgtaggccg
    14521 gataaggcgt taacgccgca tccggcataa acaaagcgca ctttgtcaac aatctgtata
    14581 cccggtggcg ctgtgcctct gcgtgcagcg ccatccggca gcaatacttc attctctgtc
    14641 cgacaagggc gggaattgat ttgtgagcaa tacagacacg cagttccagg ctgcgagtca
    14701 ctatagagaa tccgggttat gttgcagttt attctccgac gtttgggact cgtcatcccc
    14761 acgtttatcg gtattaccct tctcacattt gcctttgtcc acatgatccc gggcgatccg
    14821 gtgatgatca tggcaggcga acgtgggatc tcccctgagc gtcacgtgca gctgctggct
    14881 gaactcggct tagataaacc gatgtggcag cagtatctcc attacatttg gggcgtaatg
    14941 cacggcgatc taggcatttc aatgaaaagc cgaattccag tatgggaaga gttcgtgccg
    15001 cgctttcagg ccacgctgga acttggcgtc tgcgcgatga tttttgctac ggcagtcggt
    15061 attccggttg gtgtgctggc tgcggttaaa cgcggttcca ttttcgatca cactgcagtt
    15121 ggcctggcgc tgaccggcta ctcgatgccg atcttctggt ggggcatgat gctgatcatg
    15181 ctggtttcgg tgcactggaa cctgacgccc gtctccggtc gcgtgagcga tatggtgttc
    15241 ctcgatgact ccaatccgtt aaccggtttt atgctaatcg acaccgccat ctggggtgaa
    15301 gacggcaact ttatcgatgc cgtcgcccat atgatcttgc ccgccattgt gctgggtact
    15361 attccgctag cggtcattgt gcgtatgaca cgctcctcga tgctggaagt gctgggcgag
    15421 gattacatcc gcaccgcgcg cgccaaaggg ctaacccgca tgcgggtgat tatcgtccat
    15481 gcgctgcgta acgcgatgct gccggtagtg accgttatcg gcctccaggt gggaacattg
    15541 ctggcggggg cgattctgac cgaaaccatc ttctcgtggc ccggtctggg gcgctggttg
    15601 attgacgcac tgcaacgccg cgactatccg gtagtgcagg gcggcgtatt gctggtggcg
    15661 acgatgatta tcctcgtcaa cttgctggtc gatctgctgt acggcgtggt gaacccgcgt
    15721 attcgtcata agaagtaagg ggacatcatg tcacaggtta ctgaaaataa agtgattagc
    15781 gcaccggtgc cgatgacccc gttacaggag ttctggcact attttaaacg caacaaaggc
    15841 gcggtcgtcg ggctggttta cgtcgccatc gtgctgttca tcgcgatctt tgccaactgg
    15901 attgcgcctt ataacccggc ggatcagttc cgcgatgcgc tgctcgcccc gccagcctgg
    15961 caggaaggcg gcagtatggc acacttgctg ggcaccgatg acgtcggtcg tgatgtgctg
    16021 tcgcggctga tgtacggcgc gcgtttatcg ctgctggttg gctgtctggt ggtggtgttg
    16081 tcgctgatta tgggcgttat tctcggcctg atcgccggtt acttcggcgg cctggttgat
    16141 aacattatca tgcgcgtggt cgatatcatg ctggcgctgc caagtctgct gttggcactg
    16201 gtgctggtgg cgattttcgg cccgtcgatt ggtaacgccg cgctggcgct gaccttcgtt
    16261 gccttgccgc actatgtgcg cttaacccgc gccgccgtgc tggtggaggt gaatcgcgat
    16321 tacgtcaccg cctctcgcgt ggcaggtgcc ggggcgatgc gccagatgtt tattaacatc
    16381 ttcccgaact gccttgcgcc gctgattgtt caggcgtcgc tcggtttctc taacgccatt
    16441 ctcgatatgg ctgctcttgg tttcctcggc atgggggcac agccgccaac gcctgagtgg
    16501 ggcaccatgc tctccgacgt gttgcagttc gcgcaaagcg cctggtgggt cgtgaccttc
    16561 cctggtctgg cgatcctgtt gacggtgctg gcatttaacc tgatgggtga cggtctgcgt
    16621 gacgcgctcg atcccaaact aaagcagtaa gaggttcgag atggcgttat taaatgtaga
    16681 taaattatcg gtgcatttcg gcgacgaaag cgcaccgttc cgcgccgtag accgcatcag
    16741 ctacagcgta aaacagggcg aagtggtcgg gattgtgggt gagtccggct ccggtaagtc
    16801 ggtcagttca ctggcgatta tggggctgat tgattatccg ggccgcgtaa tggcagaaaa
    16861 actggagttt aacggccagg atttgcagcg tatctcagaa aaagagcgcc gcaacctggt
    16921 gggtgccgaa gtggcgatga tcttccagga cccgatgacc agccttaacc cgtgctacac
    16981 cgtgggtttc cagattatgg aagcgattaa agtgcatcag ggcggcaaca aaagtacccg
    17041 ccgtcagcgg gcgattgacc tgctgaatca ggtcggtatt cccgatccgg cttcgcgtct
    17101 ggatgtttac ccgcatcagc tttccggcgg catgagccag cgcgtgatga tcgccatggc
    17161 gattgcctgt cggccaaaac tgctgattgc cgatgaaccg accaccgcgc tggacgtgac
    17221 cattcaggcg caaatcatcg aactactgct ggagctacag cagaaagaga acatggcgct
    17281 ggtgttaatt acccatgacc tggcgctggt ggcggaagct gcgcataaaa tcattgtgat
    17341 gtatgcaggt caggtagtag aaacgggcga tgcacacgcc atcttccatg cgccgcgtca
    17401 cccgtatact caggcattgc tgcgtgcgct gccagaattt gctcaggaca aagaacgtct
    17461 ggcgtcgttg ccaggtgttg ttcccggcaa gtatgaccgc ccgaacggct gcctgcttaa
    17521 cccgcgctgc ccctatgcca ctgacagatg tcgcgctgaa gaaccggcgc tgaatatgct
    17581 cgctgatggg cgtcagtcca aatgccatta cccacttgat gatgccggga ggcccacact
    17641 atgagtacgc aagaggccac ctcgcaacaa ccgctgttgc aggctatcga cctgaaaaaa
    17701 cattatccgg tgaagaaagg catgttcgtg ccggaacgtc tggtgaaggc gctggacggc
    17761 gtttcgttta accttgaacg tggcaaaacg ctggcagtag tgggcgaatc tggctgcggt
    17821 aaatcgaccc tcggtcggtt gctgacgatg atcgaaacgc ccaccggcgg cgagctgtat
    17881 tatcaggggc aggatctgct caagcacgat ccgcaggcgc agaagctgcg tcggcagaaa
    17941 atccagatcg tcttccagaa tccctacggt tcgctgaatc cgcgtaagaa agttgggcaa
    18001 attctcgaag agccgctgct tattaacacc agcttaagca aagaacagcg tcgggaaaaa
    18061 gccctgtcga tgatggcaaa agtcggcctg aaaaccgagc actatgaccg ctatccgcac
    18121 atgttttccg gcggacaacg tcagcgtatc gctattgccc gtggtctgat gctcgacccg
    18181 gatgtggtga ttgccgatga gccggtttct gcgctggacg tctcagtgcg tgcgcaggtg
    18241 ctgaatctga tgatggattt gcagcaggag ttggggcttt cttatgtctt tatctcccac
    18301 gacctgtcgg ttgtggagca cattgctgat gaagtgatgg tgatgtacct aggccgctgc
    18361 gtggagaaag gaacgaagga ccaaatcttc aataacccgc gccatccgta cacccaggcg
    18421 ctgctctccg cgacgccacg cctgaacccg gacgatcgtc gtgaacgcat caaactcacc
    18481 ggtgaactgc caagcccgct gaatcctccg ccgggttgcg ccttcaacgc ccgctgtcgt
    18541 cggcgcttcg gcccctgcac ccagttgcag ccgcagctaa aagactacgg aggtcaactg
    18601 gtagcttgtt ttgctgttga tcaggatgaa aatccgcagc gttaactcac aaaaccgggg
    18661 ccatgcgctc cggtttactc aatcagcttc aacagtggcg acaagcatag caaaatgccg
    18721 taaagcagaa tcagccaggc tttaaatccg cgaagttttt ccagttgtgc gactttatag
    18781 atcaggaaaa acggaatgag gcacgacaca atgccatata acgggcttcc caactgaaag
    18841 aacaccagca ctgatacacg aaacgaaacc caaatcgtca acgtaatgac gataaaagcg
    18901 cagatcgcca gagtcagcat gcgtgagtta attttcttgg tatcaataat tcggcttaac
    18961 agattgagaa taatgccttt aatagcctcg tggaaaccga gataaatgcc aaagaatgcg
    19021 gtcagtacgg caaagatatt cagcaccgta gaggtgatat gaatgatatg tccagggatc
    19081 acctgcgcgg ccagtgccag tgccgagata ttttgttcaa aggcagaaac ggcttcttcg
    19141 tggctaattg agaaggtaaa cgaaaaggca aaaaacagga tcaccgcgat gagcgtgata
    19201 taactaattc ggtgggtacg cagcgcgagc cgggttgcca gtactttatc tgcttcccgt
    19261 ttacgatagg caatattcat tgggttaagt acctgaataa ataccgcaga aaagaagcaa
    19321 aatggaatgg taagcaggac atcgcggaaa aagacggagg cttgcgggaa ggcggttata
    19381 ttggcgaaat tccagtgcgg gatcatcgca aaaccgaaca cgacaataat ccctactttg
    19441 accaccacca ttggcccaga aatcttaaac agtaatcgtt caccaccaga cgcaatcgct
    19501 atcagtacgg cgaaaatagc gactttataa agtagagatt gtgaaagatc ggcatcggtt
    19561 aaaccgaagg ttttcaggta cgaggcgctg tcgaaaacca cggagagaga gtagataaaa
    19621 atcccgtgga taatcatcaa aaagtagata accccgagga aaattcccca gttcttcccc
    19681 aggtaatgac tgataatatc ggtgtagtca ttacaggaat cgctttcaga aagggttttt
    19741 aaataaatgt cctgcactac ccaggtgaca ggataagcaa tgatcgctgc ggtaataaat
    19801 acccaaattc ccttcaagcc aatttgtact ggcatcagca cggttccggc accaatcgcc
    19861 atgcctatgc ataataaaac ccagccgaag tcgtagcgtg taaacggcaa tttctgattg
    19921 tgtttcgata gtgtgttgtg ctgcataaag tcctgtccag aaagaagtcg gcctcgcaaa
    19981 aatgaataaa cccagtttct cttgcgcaac aggtggagaa gtcgaatacg cagtatgtaa
    20041 aaaatgaggc aacagattca ctggggaggt cgcgaggagg cgcaattcta caaaattgcg
    20101 catttcacta ccagtctgga gtcagcccgg atatgaactg caactattgc aaataaagaa
    20161 attaagctgg aaggcatcgc gcaatgagga agattgccgg gtgctgacag taaaaagcaa
    20221 tcgtagaatc agcgtcaagc cggaaggatt ccggtgaggc gcaatgttgc gggggcttaa
    20281 tccctggtgg cattggttgc tggagagaga aaacccccgc acgttgcagg tatgtacctg
    20341 acaacataac gggggctaat cttgaaccta gaccactcaa gaatagccgc gaaacgttgt
    20401 cattacaaca caggcggcta tatgacgctc gcagagctgg gcatggcctt ctggcatgat
    20461 ttagcggctc cggtcattgc tggcattctt gccagtatga tcgtgaactg gctgaacaag
    20521 cggaagtagc gtgtcatgtg ggcgtcaggc tgccgtaatg gcaatttgcg cccggaccag
    20581 gccgcagggg tgaaactctg cggccttttt cgtaagaaaa atgcaacggt tgcaagcgga
    20641 taatattctg cggaatgcgc agcacagagt gaagatgaga cggattccgg aacagaaaaa
    20701 cagtcgtaga atcagcgtca agccggaagg attccggtga ggcgcaatgt tgcgggggct
    20761 taatccctgg tggcattggt tgctggagag agaaaacccc cgcacgttgc aggtatgcac
    20821 ctgacaacac cacgggggcc attcttgcat ccaaacaata caagaatagc cgcgaaacgt
    20881 tgtcattaca atacaggcgg ctatatgacg ttcgcagaac tgggcatggc cttctggcat
    20941 gatttagcgg ctccggtcat tgctggtatt cttgccagta tgatcgtgaa ctggctgaac
    21001 aagcggaagt aacgtgccat gcgggcgtca ggctgccgta atggcaattt gcgcccggac
    21061 caggccgcag gggtgaaact ctgcggcctt tttcgtaaga aaaatgcaac ggttgcaagc
    21121 ggataatatt ctgcggaatg cgcagcacag agtgaagacg tgacggattc cggaacagaa
    21181 aaacagtcgt agaatcagcg tcaagccgga agggttccgg tgaggcgcaa tgttgcgggg
    21241 gctttatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg
    21301 cacctgacaa catcacgggg gccattcttg aacctgaaca actcaagaat agccgcgaaa
    21361 cgttgtcatt acaacacagg cggctatatg acgctcgcag aactgggcat ggccttctgg
    21421 catgatttag cggctccggt cattgctggc attcttgcca gtatgatcgt gaactggctg
    21481 aacaagcgga agtaacgtgt catgcgggcg tcaggctgcc gtaatggcaa tttgcgcccg
    21541 gaccaggccg caggggtgaa actctgcggc ctttttcgta agaaaaatgc aacgattgca
    21601 cgcggataat attctgcgga atgcgcagca cagagtgaag acgtgacgga ttccggaaca
    21661 gaaaaacagt cgtagaatca gcggcaagcc ggaaaggttc cggtgaggcg caatgttgcg
    21721 ggggctttat ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt
    21781 atgcacctga caacatcacg ggggctaatc ttgaacccaa acaactcaag aatagccgcg
    21841 aaacgttggc attacaacac aggcggctat atgacgctcg cagaactggg catggccttc
    21901 tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagtatgat cgtgaactgg
    21961 ctgaataagc ggaagtaacg tgtcatgcgg gcgtcaggct gccgtaatgg caatttgcgc
    22021 ccggatcagg ccgcagaggt taaactctgc ggcctttttc gtgcttactg cgggtaaggc
    22081 acccagtcgc cgccgttcag gcgaacgtac ggtttatctt ggtattgaat aactactgca
    22141 tttgagttct cggagaccgg tgctgtttgt ggcaacccac tggtgagttt tttccagtca
    22201 acattgtctt cggtgaaaat cttgccatca agaacgcgaa ccaccagatc ggagatagcc
    22261 aggaagctgc tcggttggtc aatgacaatc ggtgccccct gatgtggtgc cttcatgccg
    22321 aagaatttca ccccaacggg gacgtctgtg atagacgggc tagggatatc acgtaggcca
    22381 gatacctgca ttctgtcgcc cttcagcgcg ccgccgtgtt ccggcaccac gaccaccatc
    22441 actttacgac ccgatttctc cagttcagta aagaaggcgt ccagttcatc aaagaatttc
    22501 tgcgcccgcg ctttgtaatc cgctgttttg ctgacccccg gataatggtt gccgtcatgc
    22561 agtggaagcg tgttgtagaa cgtggcacta cggctgtttt tatctttttc ggtaacgtcc
    22621 agccagcggt taagcacggc ggtatcgtca taaaccggcg aaccatcaaa gcccagcaaa
    22681 ataaccggca gatttgtttg atccatcaat tcagtctgca tgccgccatt ttcgcgaact
    22741 tctttcaaaa aaccgccgaa ctggccgtta tgtcccatca tcaggtgctg ggtaaagccc
    22801 agtttcgaaa ggttatcaaa cagatagcag tcgttatttg ccggttgata cagattagtg
    22861 tgcgaagtct gcccgcagct ggcgcgcagt aaacggatcg ccgccgggcc actgtaggag
    22921 gtggcggagt taaagttctt gaaatcaata tcgaaatgcg accacagtgg atgcgacatc
    22981 aacccggcgg cttctatatc cgaccaggaa agcgaacaga tgttaatcac cagtagttca
    23041 aatggctgag catcagcggg cagcgaagac gggaaggtcg atttacgttt cgcctccgcg
    23101 ttatagaaat tattcagcca ggcgttaagg ttcgccgttg ttggcggtgc agtttgtgcg
    23161 ggcatatcac ccactaccgg tgcgccaccc gtcgccgcaa cggttgccgc tgcgttacca
    23221 cccgtcgttg ttacagtggt cgtcggttgt ccggctggcc acaaggagaa acttggtccc
    23281 gccagggtaa gtacgttcag ccatagcagt atggcaacca caaaaacggt aatgcgaatc
    23341 cattgtgaca ggaataacca ggccactaat aaaacaaaaa tggccccaat catctgccag
    23401 ttaataaagc gtgtgacaag gtcgattaaa taatcggtac tgaaccccgc cacctgcgaa
    23461 ccctggctca ttatgctttc cgggccaggc aaccagatgt catgccagaa caaagcaaag
    23521 ccgatcggca gggcaatcca gtggcgcaag cgatgcaggc tgtaacgcgg aatgggcatc
    23581 agcagaaacg cggcaaacac caaattgagg agcgggtgga agttaagata tcccgcccac
    23641 aacaggccaa acttaaccag aaaatagaag ttccagccgg aaaggccgcg ccagtattgc
    23701 cagagggaag aaggcatggc ggtattttgc gtaaattgag tcattttttg gttgccctgg
    23761 ctttttccgt gcggcgtaac gtcccggccg gtttaacata acgaggttta gcaaagaaca
    23821 aacgtaaggt gtcgcgaatg cgtcgcaaag agtgccgcgc cagatagccc agcgggaaaa
    23881 atatcagtgc gcaaacgata ataatttcaa tgatatcgct gatggtcatc atgatgagcg
    23941 ctccacagca tcatctaaca gtcgcatggg ttctggtatt cgtcgccagt ggcgaccatc
    24001 gtgctcggca ttgatgaccg gtttagaact ttgcgttaaa ggcagcggca ttccccattg
    24061 ttctggggca agcaggcgca tctgcaccag ctcggcactg atttgatcat cttcaaacca
    24121 gaccatacgg tttgagaaaa tgtcgccagt aggcaatggg aaaatatgat tcaacgcggt
    24181 atccagatcg ttaatccgac agaatgagag aaacagcacc agccgattac cgccaatggt
    24241 catgatatcg ccggtacggt tagggcgaca tagcgtcagg gcttgctcaa cgcggatacc
    24301 cggtaccgga cgtagggcaa ccagaacgcc tttaccgtgg gcaggcaaga gagggttatt
    24361 catcatgttg ttgacggcat tacagaacac atcccacttc tggaaaccac gcagtttgag
    24421 cggctgggtc attgacagca aggtagtgat atcttccggt acatagcgac taaacttctg
    24481 cccttgcacg ctttcgatca tcgtcagaca acgggagagt ggcgcattcc acggaataac
    24541 catatttgca ccgcaggcca ataacaaacg ttcatcggtg gcgcgcaggc tagcggtatt
    24601 ttcccgcacg aggattttca tcgcactacc gcgctggcga cgcagggtat gaatgctgcg
    24661 ggccagtggc tcgatttgcg cattttgctg taaagaaaag accaccgtcg ccgcctgagc
    24721 ggtacgggct tcattgaaca ggacttcgtt attgttgaac agttgccagt gttccgatag
    24781 cggcggcgca ccttccagta cagcaacatt actcagaatg cgtttttcgt cgctgcgtgg
    24841 ttggatctcc gcctcttcgc tttgaactaa tgtccagata ccattttgtt gctgaacgct
    24901 aagctgctga cgggcgctga cccctttttc gttgcaccag aaggcaatat ccagcaaatg
    24961 ttggtcaccc tgaaaacgca aactggcaag accaaaaagt gaacggtatt cctcaagcaa
    25021 caatgaaaat tgtttatcgt tattatttcc gggattaatt accaaaagcg aacaatggtt
    25081 taacctgctc catttattca ttttatccaa ccatgagcga agccgctcgg caggaatatt
    25141 ttgccatgcg ttatttgcgc aaacaagaat aaaaaggtaa ttatggggat caatagaaca
    25201 ctgcaaatca cgggccaaat agtatagacc tttttcatga tttagcattg aaaataattt
    25261 tattttttcc ggcccttgag aatcatctaa ttgaaagatt ttcgccggat cgctgtccat
    25321 gctaatgacc gcgacgtgtg cggtttcagc ctgggatgca attgtttgat tcgccagact
    25381 gatagcatct tcatggcgat cgacgttaaa ccaccagacg ccgcctgctg gcatatgtcg
    25441 cagctcatcc cataatgatg agataccgat agagaataca gggtccacaa tgtccctcat
    25501 gtcaccattt tgtctgatta tcagtttact agcgaaagct aagaaataaa cctaccattg
    25561 aaattaaaac ttatcagatt tagcatgtga atcaatttag aaggtcacaa gacgtgtgat
    25621 tttagtcttt tcgtttttaa ctttttctgt tgcgtaacgg aaagtcaaaa agtgagcaaa
    25681 ttcccgtctc gccgtaaaca ataaaccggc gaaatgctca cggttagaat tgtctcatga
    25741 atggtacggt tatttcatag ggatcaagca aaatgaataa caatgaacca gatactctgc
    25801 ctgatcccgc gataggctat atcttccaga atgatattgt ggcgttaaag caggcatttt
    25861 cactgcctga tattgattat gccgatattt cccaacgcga acagttggcc gcggcattaa
    25921 aacgctggcc gttgctggca gagtttgcgc aacaaaagta ggggattggt gaatggccgt
    25981 actgggattg cagggggtgc ggggaggcgt ggggacaaca accatcaccg ccgcattagc
    26041 ctggtcatta caaatgttgg gagaaaatgt cctggtggtc gatgcctgcc cggacaactt
    26101 gttgcgcctg tcatttaatg ttgattttac ccaccgtcag ggctgggcca gagcgatgct
    26161 ggatggccag gactggcgtg acgctgggtt gcgctacacc tcgcagctcg atttgctgcc
    26221 ttttggtcag ttatccattg aagaacaaga aaatccacag cactggcaaa cccggctgag
    26281 cgatatttgc tccggcttac agcaactaaa agccagcggg cgttaccagt ggattttaat
    26341 cgacttaccg cgtgatgcct cgcagataac ccaccagctg ctgagtttgt gcgagcactc
    26401 gctggcaatc gtcaatgtgg atgccaactg ccatatccga ctgcatcagc aagcgctgcc
    26461 ggatggcgca catattttga ttaatgactt ccgtattggc agtcaggttc aggacgatat
    26521 ttaccagctt tggttgcaaa gccagcgccg attactgccg atgctcattc atcgtgatga
    26581 agcgatggct gaatgcctgg cggctaagca accagtaggt gaatatcgca gtgatgcgct
    26641 ggcggctgaa gagatactga cgctggcgaa ctggtgcctg ttgaactact ccgggctgaa
    26701 aacgccagtc gggagtgcat catgagtatc ctgacccggt ggttgcttat cccgccggtc
    26761 aacgcgcggc ttatcgggcg ttatcgcgat tatcgtcgtc acggtgcgtc ggctttcagc
    26821 gcgacgctcg gctgtttctg gatgatcctg gcctggattt ttattccgct ggagcacccg
    26881 cgctggcagc gtattcgcgc agaacataaa aacctgtata cgcatatcaa cgcctcgcgt
    26941 ccgcgtccgc tggacccggt ccgttatctc attcaaacat gctggttatt gatcggtgca
    27001 tcgcgcaaag aaacgccgaa accgcgcagc gggcattttc aggtctgcaa aatattcgtg
    27061 gacgttacca tcaatggatg aacgagctgc ctgagcgcgt tagccataaa acacagcatc
    27121 tggatgaaaa aaaagagctc ggtcatttga gtgccggggc gcggcggttg atcctcggta
    27181 tcatcgtcac cttctcgctg attctggcgt taatctgcgt tactcagccg tttaacccgc
    27241 tggcgcagtt tatcttcctg atgctgctgt ggggggtagc gctgatcgta cggcggatgc
    27301 cggggcgctt ctcggcgcta atgttgattg tgctgtcgct gaccgtttct tgccgttata
    27361 tctggtggcg ttacacctct acgctgaact gggacgatcc ggtcagcctg gtgtgcgggc
    27421 ttattctgct cttcgctgaa acgtacgcgt ggattgtgct ggtgctcggc tacttccagg
    27481 tagtatggcc gctgaatcgt cagccggtgc cattgccgaa ggatatgtcg ctgtggccgt
    27541 cagtggatat ctttgtcccg acttacaacg aagatctcaa tgtggtgaaa aataccattt
    27601 acgcctcgct gggtatcgac tggccgaaag acaaactgaa catctggatc ctcgatgacg
    27661 gcggcaggga agagtttcgc cagtttgcgc aaaacgtggg ggtgaaatat atcgcccgca
    27721 ccactcatga acatgcgaaa gcgggcaaca tcaacaatgc gctgaaatat gccaaaggcg
    27781 agttcgtgtc gattttcgac tgcgaccacg taccaacgcg atcgttcctg caaatgacca
    27841 tgggctggtt cctgaaagag aaacagctgg cgatgatgca gactccacac cacttcttct
    27901 cgccggaccc gtttgaacgc aacctgggac gttttcgtaa aacgccgaac gaaggcacgc
    27961 tgttctatgg tctggtgcag gatggcaacg atatgtggga cgccactttc ttctgcggtt
    28021 cctgtgcggt gattcgtcgt aagccgctgg atgaaattgg cggcattgct gtcgaaaccg
    28081 tgactgaaga tgcgcatact tctctgcgtt tgcaccgtcg tggctatacc tccgcgtata
    28141 tgcgtattcc gcaggcggcg gggctggcga ccgaaagtct gtcggcgcat atcggtcagc
    28201 gtattcgctg ggcgcgcggg atggtataaa tcttccgtct cgataacccg ctcaccggta
    28261 aaggactgaa gtttgctcag cggctgtgct acgtcaacgc catgttccac ttcttgtcgg
    28321 gcattccacg gctgatcttc ctgactgcgc cgctggcgtt cctgctgctt catgcctaca
    28381 tcatctatgc gccagcgttg atgatcgccc tattcgtgct gccgcatatg atccatgcca
    28441 gcctgaccaa ctcgaagatc cagggcaaat atcgccactc tttctggagt gaaatctacg
    28501 aaacggtgct ggcgtggtat atcgcaccac cgacgctggt ggcgctgatt aacccgcaca
    28561 aaggcaaatt taacgtcacc gccaaaggtg gactggtgga agaagagtac gtcgactggg
    28621 tgatctcgcg gccctacatc ttccttgttc tactcaacct ggtgggcgtt gcggtaggca
    28681 tctggcgcta cttctatggc ccgccaaccg agatgctcac cgtggtcgtc agtatggtgt
    28741 gggtgttcta caacctgatt gttcttggcg gcgcagttgc ggtatcggta gaaagcaaac
    28801 aggtacgccg atcgaaccgc gtggagatga cgatgcccgc ggcaattgcc cgcgaagatg
    28861 gtcacctctt ctcgtgtacc gttcaggatt tctccgacgg tggtttgggg atcaagatca
    28921 acggtcaggc gcagattctg gaagggcaga aagtgaatct gttgcttaaa cgcggtcagc
    28981 aggaatacgt cttcccgacc caggtggcgc gcgtgatggg taatgaagtt gggctgaaat
    29041 taatgccgct caccacccag caacatatcg attttgtgca gtgtacgttt gcccgtgcgg
    29101 atacatgggc gctctggcag gacagctacc cggaagataa gccgctggaa agtctgctgg
    29161 atattctgaa gctcggcttc cgtggctacc gccatctggc ggagtttgcg ccttcttcgg
    29221 tgaagggcat attccgtgtg ctgacttctc tggtttcctg ggttgtatcg tttattccgc
    29281 gccgcccgga gcggagcgaa acggcacaac catcggatca ggctttggct caacaatgat
    29341 gataacgcga tgaaaagaaa actattctgg atttgtgcag tggctatggg gatgagtgcg
    29401 ttcccctctt tcatgacgca ggcgacgcca gcaacgcaac cactgatcaa tgctgagcca
    29461 gctgtagccg cccagacgga acaaaatccg caggtggggc aagtgatgcc aggcgagcag
    29521 ggagctgatg cccccatcgt ggcgcagaac ggtccttcgc gtgatgtgaa gctgaccttt
    29581 gcgcaaatcg cgccgccgcc gggcagcatg gtgctacgtg gtattaaccc gaacggcagc
    29641 attgagtttg gtatgcgcag cgatgaagtg gtgacgaagg cgatgctcaa cttcgaatac
    29701 acgccatcgc catcgttact gcctgtccag tcgcagttaa aggtttatct caatgatgag
    29761 ctgatgggcg tgctgccagt gaccaaagaa cagttgggta aaaaaacgct ggcgcaaatg
    29821 cccattaatc cactgtttat taccgacttc aaccgtgtgc ggctggagtt tgtcggccat
    29881 tatcaggacg tgtgcgaaaa cccggccagc accacgcttt ggctggatgt tgggcggagc
    29941 agtggactgg atctgaccta tcagaccctg aatgtgaaga atgacctgtc acacttcccg
    30001 gtgccattct ttgacccgcg cgataaccgt actaacacct tgccgatggt ctttgcgggt
    30061 gcgccggatg ttgggctgca acaagcatct gccattgtcg cctcgtggtt tggttcgcgt
    30121 tctggctggc gtgggcagaa cttcccggtg ctctataacc aattgccgga tcgcaatgcc
    30181 attgtctttg caaccaacga caaacggccg gacttcctgc gcgatcatcc ggcggtaaaa
    30241 gccccggtga ttgagatgat taaccatccg cagaatcctt acgtcaaact gctggtggtg
    30301 tttggtcgtg acgacaaaga cctgttgcag gcagcgaaag gtatcgctca gggtaacatt
    30361 ctgttccgtg gtgaaagcgt ggtagtgaat gaagtgaaac cgctgctacc gcgtaagccg
    30421 tacgatgcgc cgaactgggt acgtaccgat cgtccggtca catttggcga actgaaaacc
    30481 tatgaagaac agttacaatc cagcggcctt gagccagcag cgattaacgt ttcgctaaac
    30541 ctgccgccgg atctctacct gatgcgcagt accggcattg atatggatat taattaccgc
    30601 tacaccatgc cgccggtgaa agacagttcg cggatggata tcagcctgaa taaccagttc
    30661 ctgcaatcct tcaacctgag cagcaaacag gaggcgaacc gtctgctgct gcgtattccg
    30721 gtattacaag gtttgctgga tggcaaaaca gatgtctcta ttccggcgct gaaactgggc
    30781 gcgaccaacc agctgcgctt cgactttgag tatatgaacc cgatgccagg cggttcggta
    30841 gataactgta ttaccttcca gccggtgcag aatcatgtgg tgattggtga cgattccacc
    30901 atcgacttct cgaagtatta ccacttcatc ccgatgccgg atctacgcgc ctttgctaac
    30961 gcgggcttcc cattcagccg gatggcggat ctgtcgcaaa ccatcaccgt gatgccgaaa
    31021 gcgcctaacg aagcacagat ggaaacgttg ctgaatactg ttggttttat cggcgcacag
    31081 acgggcttcc cggcgattaa tctgacggtg accgatgatg gcagcaccat tcagggcaaa
    31141 gatgccgaca tcatgatcat cggtggtatc ccggacaaac tgaaagacga taagcagatc
    31201 gacctattgg tgcaggcgac cgaaagctgg gtgaaaacac cgatgcgcca gaccccgttc
    31261 cccggcattg tgccggacga gagcgatcgc gcggcagaaa cccagtcaac gctgacctct
    31321 tccggtgcga tggcggcggt gattggcttc cagtcgccgt ataacgacca gcgcagcgtg
    31381 attgcgctgt tggcagatag cccacgcggt tatgaaatgc ttaacgatgc ggtgaacgat
    31441 agcggcaaac gcgccaccat gttcggttcg gtcgcggtga tccgcgagtc cggtatcaac
    31501 agcctacgtg ttggcgacgt ttattacgta ggtcatctgc cgtggttcga gcgcttgtgg
    31561 tatgcgctgg caaaccatcc gattctgctg gcggtgctgg cgactatcag tgtgatattg
    31621 ctggcatggg tactgtggcg tctgctgcga attattagtc gtcgtcgtct taacccggat
    31681 aacgagtaat tgaagatgaa tgtgttgcgt agtggaatcg tgacgatgct gttgttggct
    31741 gcctttagtg ttcaggcagc ctgtacctgg cctgcctggg agcagtttaa aaaggattac
    31801 atcagtcagg aagggcgcgt catcgacccc agcgacgcgc gcaaaatcac cacctccgaa
    31861 gggcaaagtt acggtatgtt ctttgccctg gcggctaacg accgtgcagc tttcgataat
    31921 attctcgact ggacgcagaa caatctcgct cagggttctt taaaagaacg tttgcccgcc
    31981 tggctgtggg gcaagaaaga gaacagtaag tgggaagtgc tggacagcaa ttcggcctcc
    32041 gatggtgatg tctggatggc ctggtcgttg ctggaggcgg ggcgtttgtg gaaagagcag
    32101 cgttataccg acatcggcag cgcgttgcta aaacgtatcg cgcgggagga agtggtgacg
    32161 gtgcctgggc tgggttccat gttgttaccg ggcaaagtgg gttttgctga ggataacagc
    32221 tggcgtttta accccagcta catgccgccg acgctggcgc agtatttcac ccgctttggc
    32281 gcgccgtgga ccacgctgcg cgaaaccaat caacatttat tgctggaaac cgccccgaaa
    32341 ggcttttcgc cagactgggt gcgctatgag aaagacaaag gctggcagct aaaagccgaa
    32401 aaaacattga tcagcagcta cgacgctatc cgcgtttaca tgtgggtagg catgatgcct
    32461 gacagcgatc cgcaaaaagc gcggatgctc aaccggttta aaccgatggc gacattcact
    32521 gagaaaaacg gttatccgcc ggaaaaagtg gatgtggcta cggggaaagc gcagggtaaa
    32581 ggaccggtcg gtttttctgc cgccatgctg ccttttttac aaaaccgcga tgcgcaggca
    32641 gttcagcgcc agcgcgtggc cgataacttt cccggcagcg atacctatta caactatgtg
    32701 ctgaccctgt ttggacaagg ctgggatcaa caccgtttcc gcttctcgac aaaaggtgag
    32761 ttattacctg actggggcca ggaatgcgca aattcacact aaacatattc acgctttccc
    32821 tcggtctggc cgtcatgccg atggtcgagg cagcaccaac catcagcaga ccctgcggat
    32881 ggacagcggc aacactaatg ccgtgcgcgg gctggcgaat atttaccgcc agcagtcgcc
    32941 agaaaaagct gaagcgttta tcgcctcgct ctctgccagc cagcggcgca gcattgatga
    33001 tatcgaacgc agcctgcaaa acgaccgtct ggcacagcag gcagaggtac tggaaaacca
    33061 gggcaaatgg gcgcaggcgg cagcacttca gcggcaacga ctggcgctgg gccccggcag
    33121 cgtatggatt acttaccgac tggcaggaag aagctgcgcg gcaatccaac gatggcggca
    33181 gcagtcaggg ctttggctac acggcgcggg cattacttga acgacgtgtt acttccaact
    33241 ggtttgttgg cacggcaatt gatatccagc aggtgaaaga ttacgcaccc agccatttcc
    33301 tgctctacgt acgttattcc gccgccggat ggcagggtga catggattta ccgccgcagc
    33361 cgctgatacc ttacgccgac tggtaagttt tcagatagcg cctctcttaa tgccgctgcg
    33421 atcgggtata ctcgggcggc aatctgggat ttccgggggg agacaatttg cgcgtaagtc
    33481 gctcgttaac aatcaagcag atggcaatgg tggcagccgt tgtcctggtg ttcgttttta
    33541 ttttttgcac cgttttgctg ttccatctgg tccagcagaa tcgctataac acggctacgc
    33601 aactggaaag cattgctcgc tctgtccgcg aacccttatc ttcagctatt ttgaaaggcg
    33661 atattcccga agcggaagct attcttgcca gcattaaacc ggcaggcgtg gtcagccgtg
    33721 ccgatgtagt gctgcctaac cagttccagg cgctgcgtaa aagttttatt ccagagcgtc
    33781 cggtgccggt aatggttact cgcctgtttg agctaccggt tcaaatctcg ctgggcgtct
    33841 actcgctcga acgtccggca aatccgcagc caatagccta tctggtgcta caggcggatt
    33901 ccttccgtat gtataagttc gtgatgagca ctctctcaac gttagtgacc atttacttac
    33961 ttttgtcgct tatcctgacc gtcgccatca gctggtgcat taaccgcctg attttgcatc
    34021 cgttacgcaa tattgctcgc gaacttaacg ccatcccagc ccaggagctt gttggtcacc
    34081 aactggcatt accgcgtctg catcaggacg atgaaatcgg tatgttggtg cgcagttaca
    34141 acctcaacca gcaattgctg cagcgccatt atgaagaaca gaacgaaaat gcgatgcgct
    34201 tcccggtgtc ggatttgccg aacaaagcct tgctgatgga gatgctggag caggttgtcg
    34261 cgcgtaaaca aaccaccgcg ctgatgatca tcacctgtga aaccctgcgt gatactgcgg
    34321 gcgtgctgaa agaggcgcaa cgagaaattc tgctgctgac gctggtggaa aaactcaaat
    34381 cggtactgtc gccacgtatg atcctcgcgc agattagcgg ttatgacttt gctgtcattg
    34441 ccaacggtgt acaggaaccg tggcacgcaa tcactttggg tcagcaagta ctcactatca
    34501 tgagcgagcg cctgccgatt gaacgtattc aactccgtcc gcactgtagc attggcgtgg
    34561 cgatgttcta cggcgatctc accgccgaac agctttacag tcgcgctatt tctgcggcat
    34621 ttaccgctcg ccataaaggc aagaatcaga ttcagttctt tgatccgcag cagatggaag
    34681 cagcccagaa gcggttgacg gaagagagcg atatccttaa tgcactggaa aatcatcagt
    34741 ttgcaatttg gttacagcca caggtcgaga tgaccagcgg taaactggtc agtgcggaag
    34801 tgttactgcg tatccagcaa ccggatggca gttgggacct gccggatggc ttaatcgatc
    34861 gcattgagtg ctgtgggctg atggttaccg tcggtcactg ggtgctggaa gagtcctgtc
    34921 gattgcttgc agcctggcaa gagcgcggca ttatgctgcc cttgtcggta aacctctctg
    34981 cgctgcaact gatgcacccg aatatggtgg cggatatgct ggaactgtta acccgctatc
    35041 gcattcagcc gggaacactg attctggaag tgacagaaag ccgacgtatt gacgaccctc
    35101 atgctgcggt ggcaatcctc cgtccgctgc gcaatgccgg agttcgggtg gcgctggatg
    35161 atttcggcat gggctacgca gggctgcgtc agctgcagca tatgaaatcg ttgccaatcg
    35221 acgtactgaa aatcgacaaa atgtttgttg aaggcttgcc ggaagatagc agcatgattg
    35281 ctgcaattat catgctggcg cagagcctga acttacaaat gattgccgaa ggcgtggaga
    35341 ctgaagcaca acgagactgg ctggcaaaag cgggcgttgg tattgcccag ggcttccttt
    35401 ttgctcgccc actccctatt gaaatcttcg aagagagtta cctggaagaa aagtagctac
    35461 cccaaactga ttacaaaact ttaaaaagtg ctggtttgtg cgagccagct caaacttttt
    35521 aacctttttg tttcaattat gatccaggta catttctgtg atgttgtctg ggtgttattt
    35581 taaggccgca ggtacccccc cccataacct tacaagacct gtggttttac taaaggacac
    35641 cctatgaaaa cctctctgtt taaaagcctt tactttcagg tcctgacagc gatagccatt
    35701 ggtattctcc ttggccattt ctatcctgaa ataggcgagc aaatgaaacc gcttggcgac
    35761 ggcttcgtta agctcattaa gatgatcatc gctcctgtca tcttttgtac cgtcgtaacg
    35821 ggcattgcgg gcatggaaag catgaaggcg gtcggtcgta ccggcgcagt cgcactgctt
    35881 tactttgaaa ttgtcagtac catcgcgctg attattggtc ttatcatcgt taacgtcgtg
    35941 cagcctggtg ccggaatgaa cgtcgatccg gcaacgcttg atgcgaaagc ggtagcggtt
    36001 tacgccgatc aggcgaaaga ccagggcatt gtcgccttca ttatggatgt catcccggcg
    36061 agcgtcattg gcgcatttgc cagcggtaac attctgcagg tgctgctgtt tgccgtactg
    36121 tttggttttg cgctccaccg tctgggcagc aaaggccaac tgatttttaa cgtgatcgaa
    36181 agtttctcgc aggtcatctt cggcatcatc aatatgatca tgcgtctggc acctattggt
    36241 gcgctcgggg caatggcgtt taccatcggt aaatacggcg tcggcacact ggtgcaactg
    36301 gggcagctga ttatctgttt ctacattacc tgtatcctgt ttgtggtgct ggtattgggt
    36361 tcaatcgcta aagcgactgg tttcagtatc ttcaaattta tccgctacat ccgtgaagaa
    36421 ctgctgattg tactggggac ttcatcttcc gagtcggcgc tgccgcgtat gctcgacaag
    36481 atggagaaac tcggctgccg taaatcggtg gtggggctgg tcatcccgac aggctactcg
    36541 tttaaccttg atggcacatc gatatacctg acaatggcgg cggtgtttat cgcccaggcc
    36601 actaacagtc agatggatat cgtccaccaa atcacgctgt taatcgtgtt gctgctttct
    36661 tctaaagggg cggcaggggt aacgggtagt ggctttatcg tgctggcggc gacgctctct
    36721 gcggtgggcc atttgccggt agcgggtctg gcgctgatcc tcggtatcga ccgctttatg
    36781 tcagaagctc gtgcgctaac taacctggtc ggtaacggcg tagcgaccat tgtcgttgct
    36841 aagtgggtga aagaactgga ccacaaaaaa ctggacgatg tgctgaataa tcgtgcgccg
    36901 gatggcaaaa cgcacgaatt atcctcttaa tctcaccact tatgcccgca ctcccttccc
    36961 cgagtgcggg tgtttgcgca taattgaccc ctgtgcctga acttaccacc tgggtgagtt
    37021 caggtttatt tcacttcttc cgtgacattt tcatgttctt gcggtctaac acgaagtgtt
    37081 tttaacgtca tttagactga ccgacaacgg gcagtttttt tattaccagg atagttgatc
    37141 aggggttcac atgcagggca taaaaattcg acttttagcg ggcggtttgc tgatgatggc
    37201 cactgctggc tatgtgcagg cagatgcgct ccagcctgat ccagcatggc aacaggggac
    37261 gctttccaac ggtttacagt ggcaagtgct gaccaccccc cagcgtccca gcgatcgtgt
    37321 tgaaattcgc ctgctggtta ataccggttc gctcgccgaa agtacacaac agagcggtta
    37381 cagtcacgcc atccctcgta ttgcgctaac gcaaagcggt ggccttgacg cagcacaggc
    37441 gcgttcattg tggcagcagg ggatcgaccc taaacgcccg atgccgccgg taattgtctc
    37501 ttatgacacc acgctgttta atctgagttt gcccaataac cgtaacgatt tgctgaaaga
    37561 agcgctctct tatctggcaa atgccactgg caaattgacc atcacaccag aaaccatcaa
    37621 ccacgcgctg caaagtcagg acatggtggc aacctggcct gccgatacta aagagggctg
    37681 gtggcgctat cgtctgaaag ggtcaacctt gttaggtcac gatcctgccg atccgctgaa
    37741 acaacccgtt gaagcggaaa aaattaaaga tttctatcag aaatggtaca ccccggatgc
    37801 aatgacgcta ctggtggtgg gaaacgtgga tgcgcgctcg gttgtcgacc aaatcaacaa
    37861 aacgtttggc gaactgaaag gcaaacgtga aacgccagct ccggtgccga cgctttctcc
    37921 gctgcgtgcg gaagcggtga gtattatgac tgacgcggtg cgtcaggacc ggttatctat
    37981 catgtgggat acgccgtggc agccgattcg tgaatcagcc gcactgctgc gctactggcg
    38041 tgcggacctg gcccgcgagg cgctgttctg gcatgttcag caagcgttaa gcgccagtga
    38101 cagcaaagac atcggtcttg gatttgactg ccgtgtgctg tatctgcgtg cgcagtgtgc
    38161 catcaacatc gaatcaccaa acgacaagct gaacagcaac cttaatctgg tggcgcgtga
    38221 actggcgaag gttcgcgata aaggtctgcc ggaagaagag ttcaatgcgt tagtggcgca
    38281 aaagaaactg gagctgcaga aactgtttgc cgcctatgca cgggctgata ccgatattct
    38341 gatgggccag cggatgcgtt cgttgcaaaa tcaggtcgtc gatatcgcgc cggagcagta
    38401 tcagaaactg cggcaggatt tccttaacag cctgactgtg gagatgttaa atcaggatct
    38461 gcgtcagcag ttgtcgaatg atatggcgtt aatactgctg cagccgaaag gcgagccgga
    38521 atttaacatg aaagcgttgc aggcggcctg ggatcaaatc atggccccat ctaccgccgc
    38581 tgcgaccacc tctgtcgcca cggatgacgt acatcctgaa gtgacggata ttccacctgc
    38641 acagtaatgc ctgatggtga tgagttagca caaccatcag gtatttatgg atgagctgga
    38701 tagaggggta acactttatc cagccttttg catatgctgc gtttacgctg gcatcgcctc
    38761 acgcgggata atcgcgccgc gatactgaat aacggtactt gcggtcaggt gcccgcgttt
    38821 cgccgcgttt tccgcactgc cgcccgtcaa acgtaccgcc agataaccgg cgctgaaaga
    38881 gtcgccagct gcggtggtat cgatcacttt ttctttcggc agtttcaccg ccggaacatc
    38941 aactaacgct tcaccagcaa tggacaccag gcaagaatcc gccccgcgtt tcaccaccac
    39001 ttctttcacg cccgcgttat gggtgcgcgc aatgacgtct tccaccggct gttgacccca
    39061 cagcgcgtct tcgtcgtcca gcgtcaggaa ggcgatatcc gtgcattcca gcatttgctg
    39121 gtacacctgc tgtgtctctt ctttgctggc ccacaggcgc ggacgatagt tattgtcgaa
    39181 aatcactttt ccgcctttgg cgcggcattc gcgcagcagg gaaagcagct tttcgcggct
    39241 ggtcgggctt aagatcgcca ggctaatccc gctcaggtag agataatcga aattcgccag
    39301 ctcttcgcaa atcgccgcag actgctcgct cgccagccag aatttggcgg cggcttcgtt
    39361 acgccagtag tagaacgtac gttcgccggt gctgtcggtt tcaatgtagt aaaggcccgg
    39421 aagacggttt tccatccgtt gggtcaggga agtatcaacg ttctcgccgt gccaggcgtc
    39481 cagcatctgc tggctaaaac tgtccgttcc cagcgccgtt acgtaatgaa cggttaatgc
    39541 cgcaggatcg acctgacggg cgatatagac ggaagtgttc agggtatcgc cgccgaaacc
    39601 gcgcttaacg tccgcgcctt tctcggaaag ctcaatcatg cattcgccaa tcacggcaat
    39661 ctttttggac atagtcgtga acctgatctg taaaaaatta gcgttagttt gcgctgtggt
    39721 ggtttgctgg tcaaccatat taaaacagtg ttccattatt tttttgagcc agaacgtatt
    39781 cctgaaagat tccgttgtgg agtgaggaaa tttaccccgg ccaggcggta aagttctgcc
    39841 cttacgcgcc gataatcttt gtcgagtccg ggcagcatca cttttaaaca caggacatct
    39901 ttgatgataa ggcaggttat ccagcgaata agcaaccctg aagcaagcat cgagagcttg
    39961 caggaacggc gtttttggtt gcagtgtgag cgtgcttaca cctggcagcc gatctatcaa
    40021 acatgcgggc ggttaatggc cgtggagcta ttaacggtgg tcacgcatcc cttgaaccct
    40081 tcgcaacgcc tgccgccgga tcgctatttt actgaaatca ccgtcagcca tcggatggag
    40141 gttgtgaaag agcagattga tttgctggcg caaaaagccg acttctttgt agagcacggc
    40201 ctgctggcat cggtcaatat tgatggccct acgctcatcg ccctgcgtca gcaaccaaaa
    40261 atcctgcgcc agattgagcg tcttccctgg ctgcgtttcg aactggtgga gcatatccgt
    40321 ctgccgaaag attcaacctt tgcctcgatg tgtgaatttg gcccgctgtg gctggatgat
    40381 tttggtaccg ggatggcaaa tttctctgca ctaagtgaag tgcgttatga ctacatcaaa
    40441 atcgcgcgag aactgtttgt gatgctgcgt cagtcgccgg aaggccgcac actcttttct
    40501 cagcttttac atctaatgaa tcgctattgt cgcggggtga ttgtcgaggg cgtagaaaca
    40561 ccggaagagt ggcgtgatgt tcagaactcg cccgcattcg ccgcacaagg ctggtttctt
    40621 tcacgcccgg caccgatgga aacgctgaat acggcggttc tggcgctata agctgcctca
    40681 ttttccgccg ggctggacta tctttaggac tggcacagga agaatatgag gaagcgaacg
    40741 atgagcaagg caggcaaaat aaccgctgcg atttcagggg ctttcttgtt gttgattgtc
    40801 gtggcgatca ttttgattgc aacttttgac tggaatcgac tcaaaccgac catcaaccag
    40861 aaagtctctg cggagttgaa tcgtccgttc gctatccgtg gcaatctggg cgtggtgtgg
    40921 gagcggcaaa aacaagaaac tggctggcgc agctgggtgc cgtggcctca tgtacatgca
    40981 gaagacatca ttcttggtaa tccaccggat attcccgaag tcacgatggt gcatttgccg
    41041 cgagtagagg caacgctggc accgctggcg ctgctgacca aaacggtctg gctgccgtgg
    41101 atcaagctcg aaaagcccga cgcgcgcctg attcgcctct ctgaaaagaa caataactgg
    41161 acgtttaatc ttgccaacga tgacaacaaa gacgcgaatg caaagccgtc ggcatggtcg
    41221 tttcggctgg ataatattct tttcgatcaa gggcggatcg ccattgatga caaagtaagc
    41281 aaagcggatc tggaaatttt tgttgatccg ttaggcaagc cgctgccatt cagcgaagtt
    41341 actggatcga aaggtaaagc ggataaagaa aaggtgggcg attacatttt tggcctgaag
    41401 gcgcagggac gttataacgg tgaaccgctc acgggtacgg gaaaaatagg cggtatgctg
    41461 gcgctgcgtg gcgaagggac gccgtttccg gtacaggctg atttccgttc aggtaatacc
    41521 cgtgttgctt ttgatggcgt cgtgaatgac ccaatgaaga tgggcggtgt cgatttacgg
    41581 cttaaatttt ctggcgattc actgggtgat ctctatgaac tgacgggcgt tctgctgccc
    41641 gataccccgc cgtttgaaac ggatggtcgg ctggtagcga aaatcgacac tgaaaaatcg
    41701 tcggtctttg attatcgcgg ttttaatggg cgaattggcg atagcgatat ccacggttct
    41761 ctggtctaca ccaccggaaa gccacgacca aaactggaag gtgatgtcga gtcgcggcaa
    41821 ttgcggctgg cggacctggg accgttgatt ggcgttgatt ccgggaaagg ggcagaaaag
    41881 tcgaaacggt ctgaacagaa gaagggcgaa aaaagcgttc agcctgcggg caaagtgctg
    41941 ccttatgacc gcttcgaaac cgataaatgg gacgttatgg atgccgatgt tcgcttcaaa
    42001 gggcggcgca ttgagcatgg cagtagcctg ccgattagcg atctttctac tcatatcatc
    42061 ctcaaaaatg ctgacctgcg cctgcaaccg ctgaaatttg gcatggcggg cggcagcatt
    42121 gcggcgaata ttcatctgga aggcgataaa aagccgatgc aggggcgggc agatattcag
    42181 gctcgtcgac tgaaactgaa agaactgatg cccgatgtgg aactgatgca gaagacgctg
    42241 ggggaaatga acggtgacgc ggaactacgc ggtagcggta actcggtggc ggcgctttta
    42301 ggcaacagta acggcaacct gaaactgttg atgaatgacg ggctggtgag ccgcaacctg
    42361 atggagattg ttgggctgaa tgtcggcaac tacattgtcg gtgcgatatt tggtgacgat
    42421 gaggtgcggg tgaactgcgc ggcggcgaat ctgaatattg ccaacggcgt ggcgcgcccg
    42481 cagatttttg ctttcgatac tgagaacgcg ttgattaatg ttaccggcac ggcaagtttt
    42541 gcttcggaac agctggattt gactattgat ccggagagta aagggattcg gattatcaca
    42601 ctgcgttcgc cgctgtatgt gcgtgggacg tttaaaaatc cgcaggctgg ggtgaaagcc
    42661 ggaccgctga ttgcccgtgg tgctgttgct gcggcactgg caacgctggt aacaccggcg
    42721 gcggcgttac tggcactgat ctcaccttcc gaaggggagg ctaatcagtg tcggacgata
    42781 ttgtcgcaga tgaagaagtg atgataattg ccggatgtct gacatccggc aattaaacag
    42841 atttagattt ttggcaaagt gtgctttgtt catgccggat gcggcgtgaa cgccttatcc
    42901 ggcctacaaa tccttgtgaa ttcaatatat tgtatggaaa ttcagtcctg ataagcgtag
    42961 cgcatcaggc ttttcacact tattacaacg actgatgtcg cgtctcatgg gtcagcagca
    43021 gggcgattaa cgtcaagcca gccatcgccg ccagatataa ccccaccgca cctaacccgt
    43081 agttagcctg caaccaggct gcgatatacg gcgcaacgga agccccgaga atcgacgata
    43141 cgttgtaaga gaacgatgct ccggtgtaac gcacttctgt cggaaacagc tctggtaaca
    43201 gcgcacccat cggcccgaag gtcagcccca tcagacttaa ccccagcagc aggaaggcaa
    43261 aaaccagaat cgggttgcca gaaccgagca gcgggttaaa ggcgaacagc gcgaacagga
    43321 tgatcagcgt ggtgatgatc accatgcttt tacggcgacc aaaggcatca gccagtaatc
    43381 cggcgactgg caccatcacg ccaaaaccaa taactgccat catcagcatc cacaacactt
    43441 cgttacgcgg caggccaagc ccaactggcg cggcggcggt actgaaggtc atagagtaga
    43501 ccgtcatgat gtaaaacagc gtataggttg ccagcataat gaacgtaccc agtacggtta
    43561 cgcgaacatg tttggtcagc agcgtaccca gcgggatctt cacctgtttt ttcgctttag
    43621 cgactttctc aaacaccggc gactcatgca gcgacacgcg aacatacagg ccgataatga
    43681 ccagcaccgc cgagaagata aatggcacac gccagcccca gctcataaac tgctcatcgg
    43741 tcagtagcca ggaaagcagc aagaaagtgc cgttggcaaa gaagaagcca atcggtgcgc
    43801 ctagctgtgg gaaggagcca tacagtgcac gtttgcgcgg tggggcgttt tcagtcgcca
    43861 gcagcgccgc gccgccccat tcaccgccta agcccagacc ctgaccaaat cgagccagcg
    43921 ccagcagcag tggggcgaaa ataccaatcg tggcatagcc cggcagcaga ccaatcacca
    43981 cggtcgaaat ccccatcgtt agcaacgagg cgaccagcgt cgctttacgc ccaacgcgat
    44041 cgccaaaatg accaaaaacg gcagagccaa tggggcgcgc gacgaaggcg atggcgaagg
    44101 tggcgagcga ctgtagcgtt gctgccgttg gatcgccctg cgggaagaag atatgcggaa
    44161 acacaataac ggccgcagtg gcgtaaatgt aaaagtcgaa gaactcaatg gccgtgccaa
    44221 tgagagaggc gacaaggact ttattacgcg agttgatcgg cgtgtattct tgttcgtggt
    44281 cgagtgttgt ggctgttgct tgcataatct tttcttattt tttggctaac gaatagccat
    44341 attacgcaca gcaaaagtga tatttcaatg tgtaacaaat cgcggaaatg gtcaaaaacc
    44401 aggcaaaaag gggataatgt ttaggccagt gatttttctt ctatgaaatg cttcacataa
    44461 ttttataaat agtggataaa actggtttca ggttaaataa aagttaccaa ctggatttat
    44521 atgctggggt tatgagttgg agtgaaattg tttgctccca aatagatggc tgaagaggct
    44581 ctggggaaga gtttcaagag gttatgtgcg cattatagcc ggatgcgatg ctggcgcatc
    44641 ttatccggcc tacggggagc caggccgaat aaaatcgtat gccggataag gcgtttacgc
    44701 cgcatccggc ctattttaag gctgcgtttt ccctggcatt cgcgggtcgt ctttatattc
    44761 ggcggtggca atccacgccg cgcaaaacag cgtcaaacgg gcgaagaagt agaaaaatgc
    44821 catcagcccc agcacggaac caaatgctgc gccagagggg gatttcatca gcgatggcag
    44881 ggtgtaggtc atcacgattt taatcacttc aaaaccaata gcagccagaa atgttccgcg
    44941 aatcagggct tttttgcgcg gacggtggcg cggcaaccgc cagaagatcc agaagaaaag
    45001 cagatagttg gcgaagatgg aaatcgccag gccaatcaat cgccacgtcg gtttcagcca
    45061 ctcaatgctg ttcaggtgca gggcgctaat aatcatttgc tgcgccgaac cggcaaccga
    45121 ggtgatcgaa agcgtcacaa tcagcgcaat caacaaacca atcagtgaaa taaaatcacg
    45181 cagatatttt acccagaact tctcctgatc ttgcggcgag cgttcccaga catcgcgcga
    45241 ctgggcacga atcgcttcac gcaggttacc catccagttg atgccggaat aaagtgccac
    45301 cgccaggccg acaagcccta cagtcgtacg ctgctgaacg gcggtgttga tggtgttttt
    45361 caacgtggcg gctagtgtcg gatcgctgat gttttgcaga attttgtcga agatatcctg
    45421 tagcaacatc ggatgggagg ccagcacaaa gccccctgcg gcaaacgaca ccatcaaaat
    45481 cgggatcatc gacaaaaatg agaaataggt gatagccgca ccaaactggt tacccagccg
    45541 atcgttaaag cgttctgtcg cgcgaataag gtgcgcaata accggttggc gttggacttt
    45601 ttcggcagtg gtggtgacgg tttccagcgc ctggctaact ttgccgcctt tctcgctatt
    45661 atccaactgc ttaatcggct cgtgctccag atcctgggtg ggacgtttga tctcgttttc
    45721 ctgcgtcatc cgctcttttg tccttctgtc gtggtcattc ttaaaaagta tagtcggtca
    45781 gtccaggtgc tctttcatta cgccagccag ccactgcata aacaggttta cacgccggga
    45841 aagctcccga cgctgcgggt aaaccagcga aagggagagc ggctcggcac ggtagcctgg
    45901 caacacttca ataagccgcc cggcacgcag ggcttcgcgc acggcaatgc gcgggatctg
    45961 aataatcccc agaccggcaa gaccggcggc gagatagttt tcgctactgt ttaccgtcaa
    46021 catgccgcca gacttaaacc actggacgcc attaacgctg gcaacctcaa aacctaacgg
    46081 atgtacaccc aggtgcggtg tataacgcac tattgcgtgt gaagtcagat catcggggct
    46141 ttgcggataa ccaaagcgcg tcagatagtg cggactggca cagttgacca tggttagttt
    46201 gccgagggga cgggcgataa cgccatcttc cggtaacgcg ccagtgcgta tcacgcaatc
    46261 aaaaccatca tgaagaatat ctaccggacg gttatggcta ctcagttcca gctcaattcc
    46321 cggatgctga tagagaaatt ccgacagacg cggcagtaac aggtttttcg cgattcctga
    46381 cgggatgtcg atgcgtaatt taccgctgat actggtggca tcctgttgaa acagaccgtc
    46441 cagttcgctg agattactca acacatcttt tgcccgttga taataggtca tgccttctgg
    46501 cgtgagtttg acccgtcgcg tagtgcgctg gagaagctgg gtgccaagtt gatgttccag
    46561 tgcctgtatc tggcgcgaaa cacttccctt tggcaaagca aagaaatccg ccgcgcggga
    46621 aaaactttcc agctccgcga ctttgatgaa caactgcatt gcgtgaattt tatccatttc
    46681 tttgaccgat tgttgttcac agcgaaacag taacgcgcat atcgcattgt ttattgtttt
    46741 ttctaacatc taatgccggg tttcacaatg ttgtcatgac gaatgtggcg atttctcagc
    46801 aggaaaaggg cggaatagct ttatgagaaa ggtgaatccc cttaaacaga gcgtattttt
    46861 cagtgattcc ctttaaagtg aatagaggac gtattggcta tttgtgctta tttttgtctc
    46921 attgccattt gttattttgg cttgtagtcg cggaatttca tttttaattg catgcgtact
    46981 aaccaccaat ataaggaaat cctcaaaatt gttttaaata gtgccctcga catgtaattt
    47041 caggcgttta gctgtaacta tttatatcgg taactatttt ctacgcgtca tctctgtaga
    47101 atggccaccg ccaaatggcg aattcttatt ttttctcgcg ggtaataccc ttccactact
    47161 taagtctttt taacgtattc aggtggagat tcagttatgc aaatagtcat gtttgacagg
    47221 cagtcaatat ttattcatgg aataaaaatc agtttacagc agcgtattcc aggagtgagt
    47281 attcaggggg tcagtcaggc agacgagtta tggcaaaagc tggaaagtta ccctgaagcc
    47341 ttagttatgc tcgatggcga tcaggatggt gagttttgct actggttgct gcaaaaaacc
    47401 gtggtgcagt ttcctgaggt taaggtgtta atcacggcga cggattgcaa taaacggtgg
    47461 ttacaggaag ttatccattt taatgtgctg gccattgtgc ctcgcgattc aaccgtcgag
    47521 acatttgcgt tggcggtaaa tagtgcggcg atggggatga tgtttctgcc tggagactgg
    47581 cggacgacgc cggaaaagga cattaaagat ctcaaatcgt taagcgcccg ccagcgggag
    47641 attttaacca tgttagcggc gggcgaatca aataaggaga tcggcagagc gctgaatatc
    47701 agtaccggaa cggtaaaagc gcatctggaa tccctgtatc gccgtctgga agtgaaaaat
    47761 cgcacccagg cggcaatgat gttaaatatc tcctcctg
//
LOCUS       DAETQL010000026        47512 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.026, whole genome shotgun sequence.
ACCESSION   DAETQL010000026 DAETQL010000000
VERSION     DAETQL010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.026"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            172..654
                     /gene="sseA"
                     /locus_tag="K0W65_001550"
                     /pseudo
     CDS             172..654
                     /gene="sseA"
                     /locus_tag="K0W65_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="K0W65_001551"
     CDS             complement(1473..2249)
                     /gene="sseB"
                     /locus_tag="K0W65_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBI3094857.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="K0W65_001552"
     CDS             complement(2391..3674)
                     /gene="pepB"
                     /locus_tag="K0W65_001552"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBI3094858.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="K0W65_001553"
     CDS             complement(3733..3933)
                     /gene="iscX"
                     /locus_tag="K0W65_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBI3094859.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="K0W65_001554"
     CDS             complement(3945..4280)
                     /gene="fdx"
                     /locus_tag="K0W65_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBI3094860.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="K0W65_001555"
     CDS             complement(4282..6132)
                     /gene="hscA"
                     /locus_tag="K0W65_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBI3094861.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="K0W65_001556"
     CDS             complement(6149..6664)
                     /gene="hscB"
                     /locus_tag="K0W65_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBI3094862.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="K0W65_001557"
     CDS             complement(6760..7083)
                     /gene="iscA"
                     /locus_tag="K0W65_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBI3094863.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="K0W65_001558"
     CDS             complement(7100..7486)
                     /gene="iscU"
                     /locus_tag="K0W65_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBI3094864.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="K0W65_001559"
     CDS             complement(7514..8728)
                     /gene="iscS"
                     /locus_tag="K0W65_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBI3094865.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="K0W65_001560"
     CDS             complement(8840..9328)
                     /gene="iscR"
                     /locus_tag="K0W65_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBI3094866.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="K0W65_001561"
     CDS             complement(9598..10338)
                     /gene="trmJ"
                     /locus_tag="K0W65_001561"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBI3094867.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10457..11260
                     /gene="suhB"
                     /locus_tag="K0W65_001562"
     CDS             10457..11260
                     /gene="suhB"
                     /locus_tag="K0W65_001562"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBI3094868.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11405..12259
                     /locus_tag="K0W65_001563"
     CDS             11405..12259
                     /locus_tag="K0W65_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI3094869.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12450..13730
                     /gene="csiE"
                     /locus_tag="K0W65_001564"
     CDS             12450..13730
                     /gene="csiE"
                     /locus_tag="K0W65_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBI3094870.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13722..14861)
                     /locus_tag="K0W65_001565"
     CDS             complement(13722..14861)
                     /locus_tag="K0W65_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBI3094871.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="K0W65_001566"
     CDS             complement(15021..15911)
                     /gene="hcaR"
                     /locus_tag="K0W65_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HBI3094872.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            16047..17408
                     /locus_tag="K0W65_001567"
     CDS             16047..17408
                     /locus_tag="K0W65_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HBI3094873.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17405..17923
                     /locus_tag="K0W65_001568"
     CDS             17405..17923
                     /locus_tag="K0W65_001568"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HBI3094874.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17923..18243
                     /locus_tag="K0W65_001569"
     CDS             17923..18243
                     /locus_tag="K0W65_001569"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HBI3094875.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18240..19052
                     /gene="hcaB"
                     /locus_tag="K0W65_001570"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18240..19052
                     /gene="hcaB"
                     /locus_tag="K0W65_001570"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HBI3094876.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            19062..20269
                     /locus_tag="K0W65_001571"
                     /pseudo
     CDS             19062..20269
                     /locus_tag="K0W65_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            20366..20788
                     /locus_tag="K0W65_001572"
     CDS             20366..20788
                     /locus_tag="K0W65_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBI3094877.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20836..21708)
                     /locus_tag="K0W65_001573"
     CDS             complement(20836..21708)
                     /locus_tag="K0W65_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBI3094878.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21720..22814)
                     /locus_tag="K0W65_001574"
     CDS             complement(21720..22814)
                     /locus_tag="K0W65_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBI3094879.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22847..23845)
                     /locus_tag="K0W65_001575"
     CDS             complement(22847..23845)
                     /locus_tag="K0W65_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3094880.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23870..25381)
                     /locus_tag="K0W65_001576"
     CDS             complement(23870..25381)
                     /locus_tag="K0W65_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBI3094881.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25404..26386)
                     /locus_tag="K0W65_001577"
                     /pseudo
     CDS             complement(25404..26386)
                     /locus_tag="K0W65_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26483..29584)
                     /locus_tag="K0W65_001578"
     CDS             complement(26483..29584)
                     /locus_tag="K0W65_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HBI3094882.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29591..29764)
                     /locus_tag="K0W65_001579"
     CDS             complement(29591..29764)
                     /locus_tag="K0W65_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094883.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29882..31075
                     /locus_tag="K0W65_001580"
     CDS             29882..31075
                     /locus_tag="K0W65_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBI3094884.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31273..32526)
                     /gene="glyA"
                     /locus_tag="K0W65_001581"
     CDS             complement(31273..32526)
                     /gene="glyA"
                     /locus_tag="K0W65_001581"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBI3094885.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32853..34043
                     /gene="hmpA"
                     /locus_tag="K0W65_001582"
     CDS             32853..34043
                     /gene="hmpA"
                     /locus_tag="K0W65_001582"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBI3094886.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(34088..34426)
                     /gene="glnB"
                     /locus_tag="K0W65_001583"
     CDS             complement(34088..34426)
                     /gene="glnB"
                     /locus_tag="K0W65_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBI3094887.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34487..35821)
                     /gene="glrR"
                     /locus_tag="K0W65_001584"
     CDS             complement(34487..35821)
                     /gene="glrR"
                     /locus_tag="K0W65_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBI3094888.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35811..36524)
                     /gene="qseG"
                     /locus_tag="K0W65_001585"
     CDS             complement(35811..36524)
                     /gene="qseG"
                     /locus_tag="K0W65_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBI3094889.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36689..38116)
                     /gene="qseE"
                     /locus_tag="K0W65_001586"
     CDS             complement(36689..38116)
                     /gene="qseE"
                     /locus_tag="K0W65_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBI3094890.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38674..42561)
                     /gene="purL"
                     /locus_tag="K0W65_001587"
                     /gene_synonym="purI"
     CDS             complement(38674..42561)
                     /gene="purL"
                     /locus_tag="K0W65_001587"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBI3094891.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42820..44376
                     /gene="mltF"
                     /locus_tag="K0W65_001588"
     CDS             42820..44376
                     /gene="mltF"
                     /locus_tag="K0W65_001588"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBI3094892.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44373..44909)
                     /gene="tadA"
                     /locus_tag="K0W65_001589"
     CDS             complement(44373..44909)
                     /gene="tadA"
                     /locus_tag="K0W65_001589"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBI3094893.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44934..45569)
                     /gene="yfhb"
                     /locus_tag="K0W65_001590"
     CDS             complement(44934..45569)
                     /gene="yfhb"
                     /locus_tag="K0W65_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBI3094894.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45778..46626
                     /locus_tag="K0W65_001591"
     CDS             45778..46626
                     /locus_tag="K0W65_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBI3094895.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46965..47512)
                     /locus_tag="K0W65_001592"
                     /pseudo
     CDS             complement(46965..47512)
                     /locus_tag="K0W65_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11331 a        13020 c        11950 g        11211 t
ORIGIN      
        1 acgttacaga cggtcccggc aaaagtgcct gacctgaccc ctattagcgg ttaccagcgc
       61 cccaccagac ccggtgacat ccccccccgg atcatggacg actgggggca gtaatcatgc
      121 cgggtctggc tggctaacac cccattataa ggggtacgaa taaagtaacg gggacttgca
      181 ggctggcagc gcgatgacct gctattagaa gaaggtgcag tagagctgcc ggaaggagag
      241 tttaacgccg cgtttaatcc tgaagcagtg gtgaaagtaa ccgatgtgtt attggcaagc
      301 catgaaaata cggcgcaaat tattgatgcc cgcccggctg cacgttttaa cgcagaagtt
      361 gatgaacctc gcccaggttt acgtcgcgga catattcccg gtgcattgaa cgttccgtgg
      421 acggaactgg tgcgcgaagg cgaactaaaa acgaccgatg aactggatgc gatatttttt
      481 ggtcgcggcg tcagctatga caaaccgatt atcgtcagct gcggctctgg tgtaacggca
      541 gccgtggttt tgttagcact cgcgacgctg gatgtgccaa acgtgaaact gtacgacggc
      601 gcatggagtg aatggggcgc gcgggcagat ttaccggttg agccagtgaa ataagtatat
      661 tacaggcaat aaaaaaccgc cgaatttggc gtttttttat tgctggtctg gttcgcggcc
      721 tttccagcag gttgacttgt gttacatgag caacgcaggt gcttcacagc aaaacaatac
      781 tcaccagtaa ctctcttttt gtcaagcaaa agagagtaat tattgtttat ttagcgtatt
      841 atcgacaccg gccctttccg ccgtgttcgg taataaaata acctggctta ttagtccgaa
      901 ttcagacaaa tataaacaaa tcctgctcaa aattaaaaat tctaaccggt aaaagatatt
      961 acttaaacat gtaaatttac tttcctttaa aaaacaaaaa accgccaaaa tcaggcggtt
     1021 atttgttgct ggtccggttc gcggcctttc cagcaggttg tattaccgta gtaatgcaag
     1081 cgcgtctcag cggagacaat actcgccagt aactctcttt ttgtcaagca aaagagagtt
     1141 attattgttc tgttagtgta ttatccactg cggccctttc cgccgtctcg caagcgggcg
     1201 ctggcttaag gaaaggatgt tccgcgaccg ttagagcaag cgcgtctcag cgcttgctat
     1261 cgcggcaata tcgccagtgg tgctgtcgtg atgcggtctt cgcatggacc gcacaatgaa
     1321 gatacggtgc ttctgtatcg tacttattgt ttctggtgcg ctgttttccc aagtaaataa
     1381 taaccggagt ctctccggcg acaatttact ggtggttaac aaccttcaga gcagcaagta
     1441 agcccgaatg ccgcccttta gggcggcata ttttagatta tccggttttg tttaaagtca
     1501 cgcaaaaaac caccccagcg acgttcatag aatggcgcaa tatgttcggt aataaagtgg
     1561 ctaattcctt tttccccttt tttcacctga caaatatcga ttggttcatc gccaggtaat
     1621 gtatcggtcg ctacacttcc cgtcgcctga ataatttctt cgatatcacc atcggcttca
     1681 atgccaataa gtaaattagg ctgtgcctct tcgttctctt taattgaaca aataaaagca
     1741 cgcttcaccg gcttaatggt tttaaataag gtggtgagtg aatcaatcat ttgtgctggc
     1801 ggctctgcga cttccgataa tatcagcgat tcaccgcctt ccaggatttc ctggctgctc
     1861 agcggatttc cctcttcacc aatcaacaaa ctgatttcac gcggcataaa ttctttaccg
     1921 gtcggcagtt tggcattaag gaagagcgtt tcgccaaggg tcatctcaaa caatgtgcga
     1981 acgggcatta cgacaaatgc ctgttcgtct tcaaccgcct gttgaagcgc ttctaacgag
     2041 gtgaaaaaag gaatgacgct ggtgccgtct tctttttccc agtgctgtaa atcaagcgca
     2101 ctatcttcaa ccacagcctc gccctgcgcc gccgtaccag gcacccagac ggtggattcc
     2161 agtagagtac ggaaaaaggc cgggcggtgc gccggttcgg ttgctgcttt ttccagcagg
     2221 tcttcaagtt cgttttttgt ttcggacata agaaccacaa ttcattcaac gttcggggcg
     2281 caaatgcctg atgcgctacg cttatcaggc ctacaaggga ttcgcaattt gttgaatttg
     2341 cagaatttgt aggccggata aggcgtttac gccgcatccg gcatattagt ttacgccgtt
     2401 aacagattag ctatcgtgcg cacaccaagt cccgtagcgc ccgcagacca ctgttcaacc
     2461 ggcgctttac ggtaagtcgc cgagcagtcg atatgcagcc agccttgctg atagttctca
     2521 acaaagtgcg acaggaagcc cgccgccgtg ctcgcgcctg ccggatacgc cgcgcttccg
     2581 gtattgttca gttcggcaaa gttagacggc agctggctgc ggtggaattc cgccagcggc
     2641 agacgccaga atggttcgtt ctcttgtgag gcactcgcca gtaaacgacc ggcaagcgca
     2701 tcgtcaaaac tgaacagcgc gtgataatca ttacccagcg cagttttcgc cgccccggtg
     2761 agggtcgccg catcaatgat catttccggt ttctgcgcac tggcatcaat cagaccatcg
     2821 gcaagcacca gacgcccttc ggcatcagtg ttcatcactt caactttttt accgttgcga
     2881 taggtgatga tatcgcccag cttgaacgca ttgccgctaa tcaggttatc cgcacagcag
     2941 aggaacagct tcacgcgctt gttcagtccg cgcataatgg caaatgccag cgccccggta
     3001 accgttgccg cgccgcccat gtccgacttc atcgagtcca taaacgcagt ctgtttgatg
     3061 ctgtagccgc cggagtcaaa agtgatacct ttacctacca ggcacgcgta caccggtgct
     3121 tctttatcgc cagttgggtt gtaatccagc gccagcaata ccggagaacg ttctgaaccg
     3181 cgcccgacag tgtgcagccc catataacct tgctcacgca gatcttcgcc tttggtgatc
     3241 cgataagtca cacgatcgcc cgcgacgttg ctgatcagat caacagcacg ctgtgccagt
     3301 tgcgatggtc ccaattcttc tgccggtgcg ttgatggtgt cacgcaccca gtcgatgatc
     3361 atcaggcggt tatccagttc ctggcgctgg gcatcgtcca gatccggcca ctccacttta
     3421 cgcgtgcctt tcgggccttt ataaccttgc cagaatgccc agcagcgatc cgcatcccag
     3481 ccttcaccgc ttaactgaac atgcttgatg cccagaccgt caatcttgcg ggccgcacgc
     3541 tggatcagcc ccagatcgtc tgccccgttc aaatgcaggg taatgccgtc attattaatg
     3601 ctgtaagttg ctttttctcc ccagcgcgcg tcggcaggtt gggtagagag ggtaatcttc
     3661 atcgcttctg tcattttagt tatccttatt cgtttaacag caaaacgggc tacccccagg
     3721 cagcccgttc attcactcgg cttcgtccag ccagactaac aaaatcgctt cgaggatttt
     3781 ctcgttggat gcctgcgggt cgtcgtcgaa atcttccaga tcgcaaatcc actgatgcat
     3841 atcggtgaat cgaaccgttt tcggatcaag atcgggatac gcatcgtaca gtgcttcgcc
     3901 aatttcgcgg ctatcggtcc acttaagtcc catactaacc tctgttaatg ctcacgcgca
     3961 tggttgatag tgtaacgcgg gatttcgacc actaaatctt cgtcggtgac gcgcgcctgg
     4021 cagcttaaac ggctttccgg ctccagtccc caggctttgt ccagcatgtc gtcttcctgc
     4081 tctgagcttt ccggcagtga gtcaaaacct tcacgaacga tgcagtggca ggtggtgcaa
     4141 gcacaggatt tttcacaggc gtgttcaatc tcaataccgt tacgcagcgc tgcgtcgaga
     4201 atggtttcac cgctattagc ttccagaaca gcgccatcag ggcagaggtc ctgatgaggc
     4261 aaaataacaa tctttggcat attaaacctc gtccacggaa tggcctttca gcgcacgacg
     4321 aaccgactgg tccatgcggc gagcggcgaa atcctgggtt tgtttgtcta cgtttttaat
     4381 cgcttgttcg atggcgtcaa catcatcgcc ctgcgccact tcactcaggt gagcggcagc
     4441 attgtcaatg acctgacgct ctgcggcgct taacagcgcg gcatcagcag ccagcgcgcc
     4501 gtgcagactt tccagcacac gcgccgcttc tactttttgt tctgccagca tacgggcttt
     4561 tacgtcctgc tcggcatagc tcattgagtc tttgatcatc gaagcgattt cgctatcggt
     4621 cagaccgtaa gacggtttga cctgaataga cgcctcaacg ccggtggatt tctccatcgc
     4681 cgtcacgctc aaaagaccgt cggcatcgac ctggaacgtc acgcgaatat gcgcaccgcc
     4741 agccggtagc gccggaatac cacgcagcgc aaaacgcgcc agtgagcggc agtcctgcac
     4801 cagttcgcgc tcaccctgca ttacatggat agacatcgcc gtctgaccat ctttaaaggt
     4861 ggtgaaatcc tgagcgcggg ccaccggaat agtggtatta cgcggaatca ctttctccac
     4921 caggccgccc atcgtttcga ggcccagcga cagtgggatc acatcaagca gcagcatttc
     4981 gctgtctggc ttgttaccca ccagaatatc cgcctgaatc gccgcgccaa tagcgacgac
     5041 tttatccggg tcgatggaag tcagcggtgg acgaccgaaa aattcgccta cccgttcacg
     5101 caccagcggc acgcgagtag aaccgcccac catcaccact tccagcactt cgtcagcttc
     5161 tacacccgcg tctttcagcg cgcgacgaca agccagtaag gttcgtttta ccagtggcgc
     5221 gatcagttca ttgaattgtt cacggctgat ttcgccctgc cagcccgcaa cgttaacggt
     5281 cacggagtcc gcatcgctca gcgcgatttt ggctgcaatg gtggcatcca gcagttcacg
     5341 ctgaacgcgg ttatcgctac gatcaggaat gtccgcctgc tcgcgaatgt aatccgccag
     5401 cagatggtcg aaatcatcgc cgccgagcgc ggaatcaccg ccggttgcca gcacttcaaa
     5461 cacgccgcga cttaagcgca gaatggaaat atcaaacgtc ccgccaccga ggtcataaac
     5521 agcgatcacg ccttcctgac cggaatccag cccgtaggcg atagccgcag cagtcggttc
     5581 gttaagtaag cgcagaacat gcaaccccgc cagacgcgcc gcgtctttgg tgccctgacg
     5641 ctgggcatcg tcaaagtacg ccggaacggt gataactaca ccatccagct cgcctgccag
     5701 ggcttcagtt gcccgcgccg ccagtgcttt gaggatgtcc gcagaaacgc gcaccgggtt
     5761 cagtagcccc gccgccgttt caatcatcgg caggccgttt tcgctggcct ggaattgata
     5821 aggcagatgc ggatagcgtt gctggatatc agccagcgag cgccccatca ggcgtttaac
     5881 agaactaatg gtgttggcgg tgtcaagcgc tgcgttagta cgcgcgtcat aacccaccga
     5941 atgcccttgc tgttgatagt gaacaacaga tggcagcagg tgacggcctt catggtcggc
     6001 taacgtttcg gcctgaccgc tgcgcactgt cgccaccagc gagttggttg tgcccaggtc
     6061 aataccagcc gccagacgac gctgatgcgg cgcagcactc aaaccaggtt cactaatttg
     6121 taataaggcc atgtttagct tccagaaatt aaaaatcgag cagtttttct tcgagttgtt
     6181 cggcactgct ccgcagttta tcgagaaaac gcagcttacg caccgtatcc gccgccgcgt
     6241 cccacgcctc gttgtctaac tgttcaacca tcaactgatg gcgggtatca aacatctttt
     6301 tcacacgttt gataaagctt tccagccgcg cttcatcttt cgcctgttcg atctcgtcca
     6361 gctcttcgcg cagctccaac tgttccatca ggaacgcggt gtcgcgcaca gtatgctgct
     6421 cgctggcgag atcaaaaccg tgtaaagaaa gcaaatattc cgcgcgcatt aacggatgac
     6481 gcagcgtttg ccaggcctgg ttaatggttg cagattgctg tacggcggcg agttgttccg
     6541 ccaggcttcc gctggcgaat ttatcaggat gatactgacg ttgtagatcc tgaaaacgca
     6601 ggctcagtgc ctgggtatcg agttgataac gggcaggcaa gccaaagagg gtgaagtaat
     6661 ccataacatt ctcagggctg cgatccgccg cgataacggc gaatcaggta aaacaaaccc
     6721 cacgcgcagg cgaccacggt ggggttatcg gtatgcgcat caaacgtgga agctttcgcc
     6781 gcaaccacac tcatctttaa cgttcgggtt ggtgaattta aacccttcat tcaggccttc
     6841 ttttacgaag tccagctgcg taccgtccag aaattgcagg cttttgccat cgaccacgac
     6901 tttcacgcct ttgtcttcaa acacgatgtc ttccggcgtc ggttcgtcaa caaattccag
     6961 tacataagcc atacctgaac atccggaggt tctcacgccc agacgcaggc caaacccttt
     7021 gccgcggtta gccagaaagg tatttactcg cgctgctgca ctgtcgctca gtgtaatcga
     7081 cataaccaaa cctcaactct tattttgctt cacgtttgct tttatagtcc gcaatggcgg
     7141 ctttgatcgc gtcttctgcc agaatagaac agtgaatttt caccggcggc agttcaagtt
     7201 cttcagcaat atcggtgttt ttgatcgcct gcgcttcgtc gagagacttc cctttcaccc
     7261 attcggtgac cagggagctg gaagcgatag cggaaccgca gccgtaagtt ttaaaacgcg
     7321 cgtcttcaat gataccttca tcgttgactt taatctgcaa cttcattacg tcgccacagg
     7381 ccggtgcacc caccatgccg ctgccgacgt tctcgtcgtt gttatcaaag gaacccacgt
     7441 tacgcggatt ctcgtaatgg tcgataactt tttcgctgta agccattata aattctcctg
     7501 attccgatac cgattaatga tgagcccatt cgatgctgtt cagatccacg ccctgcttgt
     7561 acatttccca cagcggagaa aggtcacgca gacgaccgat ggatttacga actaactcga
     7621 tggtgtagtc gatctcttct tcagtagtaa aacgacctaa agagaaacgg atagagctat
     7681 gtgccagctc gtcgttcagc cccagcgcgc gcagcacgta ggacggttcg aggcttgctg
     7741 acgtacaggc ggaacctgaa gaaactgcga ggtctttcag cgccataatc agcgactcac
     7801 cttcaacgta gttgaagctg acgttgagaa tgttcggcgc accgtgttcc aggtcaccgt
     7861 tcaggtaaac ttcttcgata tctttgatgc cgttccacag acggttacgc aggccgcgca
     7921 gacgttccat ctcggtcgcc atctcttctt ttgcgatgcg ataggcttcg cccattccga
     7981 cgatctggtg aacaggcaga gtgccggaac gcataccgcg ctcgtgaccg ccgccgtgca
     8041 tttgcgcttc gatgcgtaca cgcggtttac gacgcacata cagcgcaccg atacctttcg
     8101 ggccatagat tttgtgaccg gagaaagaca tcaggtcaac tttcaactgg ctcaggtcga
     8161 ttggcagttt gcccacgctc tgggttgcat caacgtgata gataatgcca cgagcacggc
     8221 acatttcgcc gatagccgcg atatcctgca ccacgccgat ttcgttattt acgtgcatga
     8281 tggaaacaag gatggtgtcg tcacgcatcg ccgcttcgag ttctttcagg tcgataatac
     8341 cgttacgctg cggtgccagg taggtgactt cgaaaccttc gcgctccagc tgacggcaag
     8401 tatccagtac cgctttgtgt tcggttttgc tggtgatgat gtgcttgcct tttttctgat
     8461 aaaagttggc tgcacctttg atcgccaggt tgtcagattc ggttgcacca gaggtaaaga
     8521 cgatttcacg cggatcagcg ccgaccagat cggcaatctg attacgggcg atatctaccg
     8581 cttcttcagc ctgccagccg aaacggtgag aacgagaggc cgggttacca aaggttccgt
     8641 ccatcgtcat gaactgcatc attttctcgg caacacgcgg gtccaccggc gtggttgcgg
     8701 agtagtcgag ataaatcggt aatttcattg ctctataaac tccgtacatc actcaatgca
     8761 aggaatcagg ctaccggctg gatgtacgac cgtgtttacg gagtatttag cactccggcc
     8821 tgattctgaa ttctttttat taagcgcgta acttaacgtc gatcgcgtct tgtgtgcggg
     8881 tgcgtggcgc gtcgtgagta tgctgacgac cagacacatc cagcacttcc tggttattaa
     8941 ccagttcgcc taaagtaatg ttgttgagaa aaccggtgag acggtcgctc aaatcacgcc
     9001 acagcgcgtg ggtcaggcat ttatcgccgc cctggcagcc gcctttaccc tgacaacggg
     9061 tggcatctac agattcgtca acggcgctaa tcacttcgcc aacggcgatg ctgctggcat
     9121 ctttgcctaa cagataacca ccgcctggtc cacgtacgct ggaaaccaga ccatttttac
     9181 gcagacggga aaacagttgt tccagataag aaagggaaat tccctgacgt tcggaaatat
     9241 cagccaacgg taccgggccc gcttcagagt tgagcgcaac gtcaagcatt gcggtcacgg
     9301 catagcgccc tttagatgtc agtctcatgt cttacttcac ctcaaactcg cccctgcccg
     9361 gggtttttta ttgtaaaagt gggggtattg catagcaggg tcaagtctga cattcccgag
     9421 taaattggtc aactatttac ttgactgatt tagtcgggta tttaaccttc agtgccattt
     9481 ttttatcgtg gcgtttgtac ctgttgtcgg atgcggtgtg aacaccttat ccgacctacg
     9541 attctgtaca ttgtaggcct gataagacgc gttaagcgtc gcatcaggca accactgtta
     9601 ttcggccttg ttacctttat tctgctgctc aatagaagcc agaatcccgc gcaggatatt
     9661 caattcctgg ctttccgggc gcgcacgggt aaacagacgg cgcaatttat tcatcacctg
     9721 ccccggatgg ttttcacgga taaaaccggt tgccagcagc gtttgttcca gatgaccata
     9781 aaaacgctcc agatcatcga ccagcggata cggcgtctct tcatgctcga cctgctcgcc
     9841 attttcctga gttgccagcc aggccatacg cacttcatag gcaataacct gaaccgccat
     9901 tgccaggttc agcgaactgt attccgggtt tgccgcaatc gcgacatgat aatggcattt
     9961 ctgcaactct tcattggtca agccgacgcg ctcacgacca aacaccagcg ccaccggggt
    10021 atttgccgct tcagcgacgc ttttcagacc gcattcgcgc gggtcgagca tcggccacgg
    10081 cagcgtgcgg gagcgtgcgc tggtgcccac caccaggcta caaccggcta acgcttcgtc
    10141 gagcgtatcg acgatgtgag cattaccaat cacatcgctg gcccctgcag ccagggcaat
    10201 cgcctgggag tcgggtttca ccagtggatt aaccagccac agattggtta atcccattgt
    10261 tttcatggca cgggcaacag aacccatatt gccggtgtgt gacgtctcca ccagcacaat
    10321 tcgaatattt tgcagcattg tctttcttca tctaaagatt attcacgcat cttatcataa
    10381 aacgaagaca gatgccgatc tcgctgctat actctgcgcc gttttcccgt tctttaacat
    10441 ccagtgagag agaccgatgc atccgatgct gaacatcgcc gtgcgcgcag cgcgcaaggc
    10501 gggtaattta attgccaaaa actatgaaac cccggacgct gtagaagcga gccagaaagg
    10561 cagtaacgat ttcgtgacca acgtagataa agctgccgaa gcggtgatta tcgacacgat
    10621 tcgtaaatct tacccacagc acaccatcat caccgaagaa agcggtgaac ttgaaggtac
    10681 tgatcaggat gttcaatggg ttatcgatcc actggatggt actaccaact ttatcaaacg
    10741 tctgccgcac ttcgcggtat ctatcgccgt tcgtatcaaa ggccgcaccg aagttgctgt
    10801 ggtatacgat cctatgcgta acgaactgtt caccgccact cgcggtcaag gcgcacagct
    10861 gaacggctac cgtctgcgcg gtagcaccgc tcgcgatctc gacggtacca ttctggcgac
    10921 cggcttcccg ttcaaagcaa aacagtacgc cactacctac atcaacatcg tcggcaagct
    10981 gttcaacgaa tgtgcagact tccgtcgtac cggttctgcg gcgctggatc tggcttacgt
    11041 cgctgcgggt cgtgttgacg gtttctttga aatcggtctg cgtccgtggg atttcgcggc
    11101 aggcgagctg ctggttcgtg aagcgggcgg catcgtcagc gacttcaccg gtggtcataa
    11161 ctatatgctg accggtaaca tcgttgctgg taacccgcgt gttgtgaaag ccatactggc
    11221 gaacatgcgt gacgagttaa gcgacgctct gaagcattaa tgactcaggc gggtgatatc
    11281 actcacccgc cctcgccttt caggcgctat tccgaaatac ttcctcaccg ctttactttc
    11341 tttcgtcact ctcccaccat ctttccccga ttaatggatg ataaagaagt cgcaaggaaa
    11401 ggttatggca ctgccagtga acaaacgcgt tcccaaaatt ctgtttattc tctttgttgt
    11461 tgccttctgc gtttatttag tgccgcgcgt tgccatcaac ttcttctatt atcctgacga
    11521 taaaatttac ggtcccgacc cctggtcggc ggaatccgtc gaatttacgg ctaaggacgg
    11581 tactcgtctg caaggctggt ttattccttc ttcgacgggc cctgctgaca acgccatcgc
    11641 aaccatcatt catgctcacg gcaatgccgg aaatatgtcc gcccactggc cgctggtcag
    11701 ttggttacct gagcgtaatt tcaacgtttt tatgtttgat tatcgcgggt ttggtaaatc
    11761 aaaaggcact ccgtcccagg ccggattgct ggacgatacg caaagcgcca tcaatgtggt
    11821 gcgccatcgc agtgatgtaa acccacaaca gctggtgctg ttcgggcaga gtattggcgg
    11881 ggcgaatatt cttgctgtta ttggtcaggg tgatcgtgaa ggcatacgtg cggtgatcct
    11941 cgactccaca tttgcctctt atgcaaccat cgccaaccag atgatccccg gcagtggcta
    12001 cttacttgat gagagttaca gcggcgaaaa ttacatcgcc agcgtcagcc cgatcccgct
    12061 tttactcatt cacggtaaag ccgatcacgt tatcccatgg cagcacagcg aaaagttgta
    12121 tagcctggca aaagagccaa aacggttgat cctaatcccg gatggcgaac acattgatgc
    12181 tttttccgat cgtcacggca atgtttatcg cgaacagatg gtggacttta tccttagcgc
    12241 gttgaatccg cagaactaac ccatgatcgc tagcacgata atcattcaca aaaccacctt
    12301 aagacatgct aatccactgg tcagaacagt ttaaaatgag aaaaattctg tgacgcttgc
    12361 caacatttct gatgattagc attcccttcg ccatttccct gagcaaactt tagctattct
    12421 tatcaattat gcttatggga gatctacaga tgatgcctac gcttgctcca ccatctgtcc
    12481 tttcggctcc ccagcgccgc tgccagatct tgctgacgct ctttcagccg gggttgaccg
    12541 ccaccatggc aaccttcagc gagcttaatg gtgtggatga tgatattgcc agtcttgata
    12601 tcagcgaaac aggacgggag atcctgcgct atcatcaact cacactgacg actggttatg
    12661 acggtagcta ccgggttgaa ggtacagtgc ttaaccaacg tttgtgttta tttcactggc
    12721 tacgacgtgg tttccgtctg tgtccgtcat ttattaccag ccaattcacc cccgccctga
    12781 agagtgaact gaagcggcgc ggaattgcgc gtaactttta cgacgatacc aatctacaag
    12841 cgttagtgaa tctctgctcc cgacggctgc aaaaacgctt tgaatcgcgc gatattcatt
    12901 tcctgtgtct gtatctgcaa tattgtttgc tgcagcacca cgctggaatt acgcctcagt
    12961 ttaatccgct ccaacgtcgc tgggcggaat cctgccttga atttcaggta gcgcaggaaa
    13021 ttggacgcca ctggcagcgt cgggcgctcc agcatgtacc gcctgatgag ccactgttta
    13081 tggcattact tttttccatg ttgcgggttc ccgatccact gcgggatgcg catcagcggg
    13141 acagacaatt gcgtcagtct atcaaacgtc tggtaaacca ttttcgtgag ctgggaaatg
    13201 ttcgttttta tgatgaacag gggttatgtg atcagcttta tatccacctc gcccaggcgt
    13261 taaatcgcag tttgtttgcc atcggtattg ataataccct gccggaagag ttcgccagac
    13321 tgtatccacg cctggtacgc accacccgcg cggcgctggc cggatttgaa agtgaatacg
    13381 gcgtccatct ttctgatgag gaaagtggtc tggtcgcggt gattttcggt gcctggctaa
    13441 tgcaggaaaa cgatctgcat gaaaaacaga ttattctact gaccgggaat gatagcgagc
    13501 gagaagcgca gattgagcag cagttacgcg aactaacgtt actgccgctc aacattaagc
    13561 atatgtcggt aaaggcattt ttgcagacag gcgctccgcg tggcgcggca ctgattattg
    13621 cgccttatac catgccgtta ccgctctttt caccaccgct gatctatacg gacctgacgc
    13681 tgacaacaca tcaacaggag cagatccgca aaatgctcga atcagcatga gggaacaact
    13741 ttcgggcgca aaaacattgc cggaagcgcc accagcgcca ttacccagaa cacgccgtgg
    13801 cccagatatt gatacaggaa accggcgaaa acggtcatga tagcgatact gccgcccatc
    13861 gcgacggcag agtaaaccgc ctgtaaacgg atgacttcgc taacctggcg agcagcaata
    13921 taacgcatgg cggccaggtg gcagaccgtg aaggtgccgc aatgcagaat ttgcaccact
    13981 atcaaccacg gcaacgccgt agttgctccc ataatgcccc agcgcactac gccgcaaatc
    14041 gccgagatca acagcatatc gcgagcacta caacggcgga aaagtttatt actcagcgca
    14101 aagataatga cttccgccac cacgcccagc gaccacaaat accccaccgc cgaggccgag
    14161 tagccagctg cctgccagta aatggcgcta aaaccgtaat aggccgcatg tgccccctgc
    14221 aataaacaaa cgcaggccag aaagcgccag ttctggcgaa ccagcgccaa ccacgcagac
    14281 caaccggtgc tctcctgctg gcggcttgcc ccttgtggct gaatcgtcgg acggatgaga
    14341 aagccgagca gcatggatgc cacgcccaac gtcaacagca cgaggatcac ccgataatca
    14401 aacatagtga ccagtttgcc cgtcagcgcc gagccaatga caaacgccac cgagccccac
    14461 agtcgcactt tgccgtaatc aagcgggaac tgcttttgcc acgtattcgc cagtgcatcg
    14521 gtcaacggta ccagcggtga gaaaaagagg ttaaagccaa tcatcaccag catcagccac
    14581 gctacgtgcg cccccgccca gaaggcgaca gcaaagagaa gtggcagcag tgccagcacg
    14641 cgcaaggcgg aaatcaggcg ggaaggatcg ctgacgcggg gcgcgatgag caaactcccg
    14701 agaaaacggg caaccagacc tgcccccaat aacaggccga tggtttctgg cgttaaacca
    14761 atccctttaa gccagacgct ccagaaaggt aaaaaaatgc cgtaactaaa aaagtatgtg
    14821 aaatagccga gcgccaacca gcgcgtggat tgcaaaacca tgagtccctc ccgtcaggag
    14881 gcgttagtct ggggttaatc gaatgttggt gcaagttgaa aaataagaat attcgagcct
    14941 gcttcccgat gtctgtaacg ccagttcaca agcactaaaa aactgcgatc cgcgacgcat
    15001 gttgcaaaaa taatgcgtaa ttatgccgtt acgcttgcca aacgttcctg cacaatggcg
    15061 atgaaatcgc gtaatgccag cttcatctcc cctttcttcc acgccatcag taaagcaatg
    15121 gaaggcacat ttccggcaat gggacgaaaa acaacctgcc cggtattaaa attattcata
    15181 taaccgggta tcaaagtgac gcccagcccc atgcccacca gattcatggt caccagaata
    15241 ttcgttgcca cctggacgat atttggctgg ctgttttctt gcgcaaacca cgctttaacg
    15301 atcggcgcaa gcgaaccgga atacgccgga tcggtactga cgaaattcac gccatccagt
    15361 tgcgcggcgg tgatctcttt ttcatgtgct aaagggtgat caaccggtaa caccaccact
    15421 aatggttcgt caaaaagctc cagataatca atctccgggc tataaacagg atggcgcatt
    15481 aagccgacgt cgagttcacc acggcgaatt ttttcctcct gttgcgtggt gattaaactc
    15541 accagctcaa tcaaggtgtc tggctgtctg agacgaaaca tcggtaatac ttttggcaat
    15601 aaattcactt ccgccgatgg cacaaagcca atggttaatt gtctgtcttc ctgaacaatt
    15661 ttccgcgccc gtaatttggc attttccgct tgttcgagga ttgccagcgc gtcctggaga
    15721 aaacattctc ccgccgccgt caacgcgact ttgcgcttat ccctcaccag taacggaaca
    15781 ccgacacagt tttcaagatc gcggatctgg ctgcttaacg aaggctgtga ggtatgcagt
    15841 ttttccgccg cacgggtaaa gttcagtgcc tgcgccactg cgacgaaata gcgtaaatgc
    15901 cgtagttcca tcaccttccc cttgttatcg aaaaaacgtc tcagctggta gaaattaact
    15961 atttcacata ttagcaacca accagcaaca tccttatggc acaaaaatag aaggtcaata
    16021 catcttatct ttcaggatta aaaaatatga ccacaccctc agatttgaac atttaccaac
    16081 tgattgacac ccaaaacggt cgggtcactc cgcgtattta taccgacccg gacatttacc
    16141 aactggagct tgagcgtatt ttcggtcgtt gctggctatt tctcgcccac gaaagccaga
    16201 tcccaaaacc cggtgatttc tttaacacct acatgggaga agatgcagtt gtcgtggtgc
    16261 gccagaaaga cggcagcatc aaggcgtttc tcaaccaatg ccgccaccgg gccatgcgtg
    16321 tgagttatgc agattgtggc aacagtcgcg cctttacctg cccgtatcac ggctggtctt
    16381 atggcattaa cggcgagttg atcgatgtac cgctggaacc tcgcgcctac ccacaagggt
    16441 tgtgtaaatc ccactggggg ctaaacgaag ttccttgtgt ggagagttat aaaggactga
    16501 tttttggcaa ctgggatacc agcgcaccgg gcctgcatga ttacctgggt gacattgcct
    16561 ggtatctgga tggcatgctg gatcgtcgcg aaggcggcac cgaaattgtc ggcggcgtac
    16621 aaaagtgggt gatcaactgt aactggaaat tcccggcaga gcagttcgcc agtgaccagt
    16681 atcatgctct gttcagccat gcttctgccg ttcaggtatt aggggcgaaa gatgatggca
    16741 gcgataagcg cctcggtgat ggacaaaccg cccgcccggt gtgggaaacc gccaaagatg
    16801 cgctgcaatt tggtcaggac ggtcacggta gcggtttctt ctttactgaa aaaccggatg
    16861 ctaatgtctg ggtcgatggc gcagtttcaa gctattaccg cgaaacctat gccgaagcag
    16921 aacaacgttt aggtgaagtt cgcgccctgc gcctggcggg tcataacaat atattcccca
    16981 cgctttcatg gctaaacggc actgccacac tccgcgtctg gcatccgcgc ggccctgatc
    17041 aagttgaagt gtgggcgttc tgtattactg acaaagccgc ctccgatgaa gttaaagccg
    17101 cttttgaaaa cagcgccact cgtgcttttg gtcctgctgg ttttctcgag caggatgact
    17161 cggagaactg gtgtgaaatc cagaaattgc ttaaaggcca ccgcgcccgc aacagcaaac
    17221 tgtgtctgga aatggggctt ggtcaggaaa agcgccgcga cgacggcatt cctggcatta
    17281 ctaactatat cttttcagaa actgccgctc gtggaatgta ccaacgctgg gccgatcttc
    17341 tgagtagcga aagctggcag gaagtgctcg ataaaaccgc cgcttaccag caggaggtga
    17401 tgaaatgagt gcgcaagttt cactggagtt acatcaccgc attagccagt ttctctttca
    17461 cgaagccagt ctgctggatg actggaaatt tcgtgactgg ttggcacagc tcgacgaaga
    17521 gattcgttac accatgcgca ccacggttaa cgcgcaaaca cgcgaccgtc gcaaaggcgt
    17581 ccagccgccg acgacctgga ttttcaatga caccaaagac cagctggagc ggcgaatcgc
    17641 ccgtctggaa acgggcatgg cctgggcaga agagccgccg tcacgcactc gtcacttaat
    17701 cagcaactgc caggtaagcg aaaccgacat cccaaacgta tttgccgtga gggtgaatta
    17761 tctactttat cgggcacaaa aagagcgcga tgaaacattc tatgttggaa cgcgtttcga
    17821 caaagttcgc cgtctggaag atgacaactg gcgcttgctg gaacgggata tcgtcctgga
    17881 tcaagcggtg atcacttccc ataacctgag tgtactgttc tgatgaatcg aatttatgcg
    17941 tgtcccgttg cggatgtgcc ggagggtgag gctctccgga tagatacctc gcccgtcatc
    18001 gccttgttca acgtgggcgg cgagttttat gccattaacg atcgttgcag ccatggtaat
    18061 gcgtcgatgt cagaagggta tctggaagat gacgccacgg tggagtgccc gctacacgcc
    18121 gccagttttt gcctgaaaac ggggaaagcg ttatgcctgc ccgccaccga tccgctcacc
    18181 acttatccag tacacgttga aggtggtgac attttcatcg acttaccgga ggcgcagcca
    18241 tgagcgatct gcataacgag tccattttta ttaccggcgg cggatcggga ttagggctgg
    18301 cgctggtcga gcgatttatc gaagaaggcg cgcaagttgc cacgctggaa ctgtcggcgg
    18361 caaaagtcgc cagtctgcgt cagcgatttg gcgaacatat tctggcggtg gaaggtaacg
    18421 tgacctgtta tgccgattat caacgcgcgg tcgatcagat cctgactcgt tccggcaagc
    18481 tggattgttt tatcggcaat gcaggcatct gggatcacaa tgcctcactg gttaatactc
    18541 ccgcagagac gctcgaaacc ggcttccacg agctgtttaa cgtcaacgta ctcggttacc
    18601 tgctgggcgc aaaagcctgc gctccggcgt taatcgccag tgaaggcagc atgattttca
    18661 cactgtcaaa tgccgcctgg tatcctggcg gcggtggccc gctgtacacc accagtaaac
    18721 atgccgcaac cggacttatt cgccaactgg cttatgaact ggcaccgaaa gtgcgggtga
    18781 atggcgtcgg cccgtgtggt atggccagcg acctgcgcgg cccacaggcg ctcgggcaaa
    18841 gtgaaacctc gataatgcag tctctgacgc cggagaaaat tgccgccatt ttaccgctgc
    18901 aatttttccc gcaaccggcg gattttacgg ggccgtatgt gatgttggca tcgcggcgca
    18961 ataatcgcgc attaagcggt gtgatgatca acgctgatgc gggtttagcg attcgcggca
    19021 ttcgccacgt agcggctggg ctggatcttt aaggaagcac gatgaaagaa aaaacgatca
    19081 ttattgtcgg tggcgggcaa gcggcggcaa tggctgcggc ctcgctacgc cagcaagggt
    19141 tcaccggtga gctgcatctg ttttccgatg agcgacatct tccttatgaa cgacctccac
    19201 tctcgaaatc catgttgctg gaagattccc cgcagttaca gcaggtgtta cccgctaact
    19261 ggtggcagga aaacaatgtt catctgcatt ccggtgtaac catcaaaacg ctgggtcgcg
    19321 acacacgaga gttagtgtta accaacggcg aaagctggca ctgggatcag ctttttatag
    19381 caaccggcgc ggcagctcga ccgctgccgt tgcttgatgc actgggagaa cgctgcttta
    19441 ccctacacca tgccggtgat gccgccagac tgcgagaagt tctgcagccc gaacggtcag
    19501 tcgtgattgt cggtgccgga actattggtc tggaactggc tgccagcgcc acgcagcgca
    19561 gcgcagatgt aaggtgacag tgattgaact ggcggcaacc gtcatgggcc gtaatgcacc
    19621 accgcccgtg caacgctatc ttttacagcg ccatcagcag gctggtgtgc gcattctgct
    19681 caataatgcc attgaacatg tggtcgatgg tgaaaaagta gaactgacgc tgcaaagtgg
    19741 ggaaacgctt caggctgatg tggtgattta cggtattggt atcagcgcca acgagcaact
    19801 ggctcgcgag gccaaccttg atactgccaa tggcattgtc attgatgagg cttgccgcac
    19861 ctgcgatccc gcgatctttg ccggtggcga tgtggcaatc actcgtcttg ataatggtgc
    19921 actacaccgc tgcgaaagct gggaaaacgc caataaccag gcgcaaattg ccgctgccgc
    19981 aatgttaggg ctaccgctac cgctactgcc gccgccgtgg ttctggagcg atcagtacag
    20041 tgataactta cagtttattg gcgatatgcg tggcgatgac tggctttgtc gtggcaaccc
    20101 ggaaactcag aaggcgattt ggtttaatct gcaaaacggc gtgcttatcg gtgcggtcac
    20161 gctgaatcag ggccgtgaga ttcgcccaat tcgcaaatgg atccagagcg gcaaaacgtt
    20221 tgatgcgaaa ctgctgatag atgagaacat cgcgcttaaa tcactgtaat caggataatt
    20281 agcgaatatc tcaatgcctg gggcgtggcg aggtgcaaga gtgtgtatta cgtttaaatc
    20341 acattatctt gcaaagggat tggttatgaa cacactacgt tattttgatt ttggagctgc
    20401 ccgccccgtt ttgttattaa ttgcccgtat cgccgtggtc ttaattttca ttatttttgg
    20461 ttttcccaaa atgatgggct ttgacggtac ggtccaatat atggcctcgt tgggcgcgcc
    20521 aatgccgatg ctggcagcga ttattgcggt agttatggaa gtgcccgccg cgatattaat
    20581 cgtgcttggc tttttcaccc gtccgctggc ggtgctgttt attttctaca cgctgggtac
    20641 ggcggtgatt ggtcaccatt actgggatat gaccggcgat gcggttgggc caaatatgat
    20701 taatttctgg aagaatgtca gtatcgctgg cgcgttcttg ctattggcaa ttaccggacc
    20761 gggggcaatt tctctcgatc ggcgttagga aagatgccgg atgcggcgtg aacgccttat
    20821 ccggcatttt ataaattaca gccactctac ccgcaacgac atctctgagg ttgttgattc
    20881 ccctggtgct agcgcaatga gatcaccccc ttccgggcga tgatgctcat ccggcgcatg
    20941 gctcatcggt tccagacaga agaaatcaaa cgcatatcct ttatcaaacg cagggtcgga
    21001 aacaaagatg aaataacacg gtgcaggtgg cgtcgtttcc atgatgatgg cataaccttc
    21061 ctgcggctgc tcgatgcggg cctgaccatt ccatccggca aaaccattgt tcacccactg
    21121 acgcggcaac ggcgcgagct ggttaaaatc cagttcctgc ggtagctgct cgcaaaactc
    21181 acccgccagc cactgctccc gttccagcca gtaaccgctc gcctgcgcct gaatccgcgt
    21241 ttgtggtgac aacggaaaat aaggatgcca gccggtacca aatggcagcg tctctgcccc
    21301 ttgattggtg acagagagcg tcaccgtcag cgtatccgcc gttaaatgaa acgcctgact
    21361 tacccgatag tgatagacac cgctgcgatg ttcatacacc agacacaaac tatcatcgct
    21421 gtgcgagaca cattgccact cgcccagcca gccatcgccg tgtagatagt gcgcatccca
    21481 ctcaacattc ggttgcagtt gatactcacg cccctgccag acaaaacggt tgccgctcac
    21541 ccggtttgca aatggcacca gcggaaaaca tgaggcatcg gttgccacac cgcttttttt
    21601 accaggacgt aagagcggcg tcgtatcgcg ccagaagcct tcgattacgc cgccctggtc
    21661 agaaacgtcc agctttagcg acccgtggga taaggtatag atggtcatcc gctaactcct
    21721 taatccggga agttaatcac aactttcccg catttgccgc tcgccatcag cacataggca
    21781 tcacctgcct gttctagcga gaagcgatgg gtaatggcgt tacgcggcca cagcttccag
    21841 tcagtcaaat cgtgggcgca tttttccata tggaacagac tggtcaccca ggagccgata
    21901 atccgccgtt gatggtgcat cagatcagcg ctgacctcga attccacttt tccggtttca
    21961 ccaatgtaaa ccacccgccc ccagtcagca gtggattgca gtgccagcaa gcgacctgcg
    22021 gcattaccgg aacaatcgag cgcaacatcc gcgccaccgt gggtaagttc agcaattatc
    22081 tgcggcagac cttcggtggt tgctaaatag ccgtgatcca tcccccctaa ctgttttgcc
    22141 atcgccagac gttccggcag catatcaacg ccgatgatcc gttttgcacc gcgacctttc
    22201 gccagcatca tcgccatcat gccgaccggc cccagaccga ccaccagcac gttatcgctg
    22261 ccggaaactt cgccgcgcaa aatcccttca taggccgtgc caacgccgca actgataaac
    22321 gcaccatctt cgtagctcag cgcatccggc aggaggatca gatctttttc ttccgccagc
    22381 agatattcgg catgaccgcc gtcacgctgc cagccgtaag ccgcttttcc tttgccagta
    22441 caagagatag gaaagccgcg gcggcagttc gggcaaaaac cacagccaga aatgtgatac
    22501 accagcacgc ggtcgccctc tttaaaatgg cggcagcctt gccccatcgc cacaatctgc
    22561 ccgcacggtt catgaccgtt gataaatccc tggtataacg gtttatcggg tgccgccgcc
    22621 gtggcacggt gttgatgata gatatagtgg acatcgcttc cgcaaatccc ggaggatttc
    22681 attttgatca gtacctggtt aatccccggc gtcggcaccg caacttcccg cagatcgacg
    22741 gttgaatttc ctagtaaata agcagccagc atcgttttca taaatccctc attaacaata
    22801 cgattaattt tcatccctgc agcacccgcg ccaggggcgt taaggtttag cgtttcgctt
    22861 tactgcttcg ctgggtcagc aagatattcg ccagcaccgc caccacgatg atgacgccgc
    22921 gtaccacctg ctggaaaaag gagttaatac cgagcagcac cagaccgtta ccgattagcg
    22981 taatcaccag cacaccaagc aatgtaccga acagggaacc gcgaccgccg gaaagtgccg
    23041 taccgccgac cacgaccgcg gcgatgacgt caaactccag accgtttgcg gcacctgcgt
    23101 taccggaacc gaggcgtgcc gccaacaaaa tgccggtcac cgccgctaat aatcccgaaa
    23161 gggtaaagat aagaatgcgc actcgacgaa cgttaatacc gcacaactgc gccgccgtgg
    23221 cattaccgcc aacggcaaaa accgagcgcc cgaaggcggt ttttcggcta atgaacacaa
    23281 acagcgcaaa caacaccatc atgatcagcg cggatactgg cacaccgaga aattgtccgc
    23341 ccagccagtc cagcacctcg ttttcgtcaa tcggcactgg cagtgcgttg gtcataaaca
    23401 gccccattcc gcgcagagcg ctccacagcc ccagtgtggc aacgaaactt ggtacgttaa
    23461 acacgccgcg cagcaccccg gcgagcgttc ccatcagcgc gcctaacagc aacaccagca
    23521 gacaagcaat cgccaacgga acttcaaatt gcagcaaaaa tgccaggcac accgagacaa
    23581 aagccaccat cggcccaacg ctgacatcaa tttcaccgga gataataatc agcgtcatcg
    23641 cccaggcggc aatcccaatg gtggcggcat cgcgcagcac gttcatctgg ttattcaatg
    23701 agataaagcc aggcgcgttc agggagaaga ccagataaag aatggcaatc accaccagca
    23761 aaccgatctc attaatatgg cgactgacaa attgtttgag cgagacgctc ttgccctgcg
    23821 gcaatggtaa tgacgaagca tacatcttca ggttcctcgg taaaattcat cagtgcacag
    23881 acagaatggc ggacatcagc tcatccacat tgaccggcga gtgaaactcc tgcgagaacg
    23941 tgccgtgctg taataacagg atgcggtcac acaccagcgg caattcctcc acttcactgg
    24001 agataaacac cacgcttttt ccttcggcag ccagctcacg gacaatacgg taaatctgct
    24061 gtttggcttc gatatcgacg ccgcgcgttg gctcgtcgag caacaaaatc tggctggcgg
    24121 catagaccca acgaccgatc accacttttt gctgattgcc accagaaagc gtgccgatgg
    24181 gtgtttcgct actggcagcc ttgaccgtca tccgctgcat cacctcttcg gtcaggcggc
    24241 ggatggtgga ccattgcagc acaccgttgg tgctgatttt ttgccgattg gtcagcactg
    24301 tattttcgtc aacgcctagc cagggaatga tccccgcttc tttgcggttt tctggcgtat
    24361 agccaatgcc gcgtttcagc atgtcgccgt aatcggggcg catgattttc tcgccgttga
    24421 taacaatttc gccctgttca tacgtctcca gcccaacaat cgccttcagc aattcactgc
    24481 gccctgcccc cagcaggcca gcaatgccaa gcacttcgcc acgacgtagc gtaaagctga
    24541 tatcctccag cttgggctta tggcgtaacg cacggacttc cagtacggcc tgatccacaa
    24601 tttcctgagg ggcaaccggc gcaatatcaa cgtgatcacg ccctagcatt agcgacacaa
    24661 tatgatgcgt ggaggtgttt tcgagcatca catcgcccgc cacctgacca tcgcgcataa
    24721 cggtggcata ggaggcaatg cggcggattt cttccatccg gtggctgaca taaatcaccg
    24781 ccacgcccag tgccgacatc tttttcaccg cactgatcac cagttcaact tctgcactgg
    24841 caagcgagct ggtgggttca tcaagaatga ccacgcgcgg ctcgcccttc atcacccgtg
    24901 caatttccac cagttgcttt tgcgccgggc ttagcgttga aacaagttgt tcaggactaa
    24961 cgtcaacgcc cagcgcctgt aagcaacgtt gggcatcctg cgccatttgc aggtaatcaa
    25021 tcatgccgtt gcggcgaggc cattgaccaa ggcagaggtt ttctgccacc gtcagccctt
    25081 ccaccagact taattcctga taaaccgcac gaacccccag ttcagcggca cggcgagtca
    25141 acgtagcttc gtcaccttcc agtcgcgtct cgccaatcca gatatcaccg ctatccgggc
    25201 gttcgctgcc ggtaagcatt cgaatgagag tcgatttgcc cgcgccgttt ttacctaaca
    25261 gcgcacgaac ttcgccttta ttgagcgtga agttaacgtt atccagcgca acgacgccgg
    25321 ggtaacgctt atttcctgcc accacttttg ctaccgggac tgcctctgtt gccgtgaaca
    25381 tagggacctc tgcgaatcag cgattagggc agaccatcaa cgtgcgttgc cagccactgt
    25441 ttaccatctt ccgttttggt atagagatcg attggcacct gaatcacttt ttcaccgtcg
    25501 gcttgtttat tgataacctt caatgtttgc gcgaaaacag cattgcccat tttcttaccg
    25561 gaaatatcca ctaccgcttt cagcacctga ttgttttcca gctcctgagc aatttcggtt
    25621 gtcatatccg aaccgaaaac agcaattttt ccggcctgat tttgattacg taccgctttt
    25681 accgcgccga gtgtcgcacc gcccgactcc cccataatgg cgttgagatc cggcgtggag
    25741 ataatcagtt tttcaccaac ggaaatcgct ttatctaaaa cagtcccttc ctggttagcg
    25801 acaatttgcg cgccgggaac gcgggatttt aatacttctt caaatccttt acgtcgctgc
    25861 acacaaactt caaaggcttc gcaattgatg acggcaattt tcggctggtc aattttattg
    25921 gcaataaaat aatcggcggc agcgttaccc agtttttacc aaattccagt ggatcgccga
    25981 ccagatacgc cgagacatat ttatcgacac ccttttgatt aatacaggtg ttgtagcaaa
    26041 tcaccggaat acccgcttca ctggcgcgac gaacggtacg gctactgcca ttttcagaca
    26101 ctgccgataa aataatggca tcgacattac gcgccacgag ggtatcaaca aaggtacttt
    26161 ctttcgaaat atccccctgg gcgttagttt caattaactg tacctgtact gaagaatctt
    26221 ttgccgcatc ctgaacgccc tgacgcactc cagcgtaata tccctgggta tcaaggtata
    26281 ttgcaccaat ggtcatttct ttatccgcag ccctggcaaa tagtgcgcta cctaatagcg
    26341 tagccattaa taatagatta cgggttgttc tcattttttt aggcatagtg cctcctgtag
    26401 ggtttttatt aacaacggct tattctaatt attttgtgat gagcggcagc gcgtgccgct
    26461 caaatattac agttaatagg gtttagtgaa cgtagttaaa aataaaaggc atcacagtgg
    26521 tgaataatgc cgctttcggc caggccggat taatcgccgt caattcagcg cgagcagact
    26581 catattgcgc aacctccccg agtcccgcgg acgccagcat cgccaccatc aggcattttt
    26641 ctttatgctg ctgttgtaaa tcgccataca gcgataacag gtcaggttgt gaaacagcaa
    26701 agaaatccgc ctcaatactg gttttcgcca tctcttgcgc ccactgtttc atttcactaa
    26761 acagttgctg tgcggtttgc tgttcaccca gcagtcgcag cgccattcct tgccagaaga
    26821 gataatcaac cggctgatcg ttgtaataac tgtggatgtt aatggtgcga tcgccggtcg
    26881 ccgccagacg taaacaacgc atcgcttcag tttcatcgcc ctgcgcgttg gcgcatatcg
    26941 cctgccagaa ccagatgtcg ttatcagttt gccccggtaa acggccttcg cttaaattct
    27001 ccggataata cagcgcggca tgaagcagtt cgctggcctg ctgcggctct ctggcatcaa
    27061 gatgctgcca ggcgcgtaat aactggttga ggataaactg actggtgacc ttcccttccc
    27121 cgccttccca cgggtggaat ttacgcgtgg cgagaatgtc cgccgctttg tctgcctgac
    27181 ccgtgagatg ccacaaattg agcagttctg cggtcatgtc gtcgcgtttc agcgcaattt
    27241 ccagattatt ttccagacgc gccagtcgtt tctccggtgt ggctccactt aacttatcca
    27301 gcaaatcccg ttcgaaaagc agacgtgcat cctgcggcgc aagctgataa gcattatcaa
    27361 gataacgcgc ggccagctca taatcgtgtt tcttattcca cgcatggatc gctaacccgc
    27421 gccagccgtc ggcaaactcc ggcgacatct ctacgcaacg ttgccataag gcaatggctt
    27481 tgttgtagct acgtttgttg tagtagaagc aggccagtaa atggcgagca aaccaacact
    27541 cttcaatact ctccagcgcc gccacttctt ccagcgtatt cgggaaacgg acaaactgcg
    27601 ggaagacatc aatggctttt gcgaccagtt cgccacgttc ggctttcggc agcaagctgg
    27661 cttgcaggta aagcggcagc gtgcgctggc agtccagtgc gttcagcatc tctgccgcca
    27721 gggtgggcat tccccagtta atcagttgcc cggcggtcat cagagcgtta acgtcgcgtc
    27781 cctgacacag accgcgccac tgcgcgaggg ctgattcgct acgaccatcg aaccagttca
    27841 gccaccacag agtggcgttc agcggataat cacgcagcag tttctcgcgc tgcaaacgcg
    27901 cgttgtcctg acgaccactt aacaccagca gcagattatg caagcaaagc acttcctgat
    27961 tggttgggca ggcacgaaga ctttgttggc aaaaatccag acccgcgtcg aagttatcat
    28021 tacgcgccgc cagtcgtgcc agaccataat agccgcctgc tttactattg ccgctccaga
    28081 ccgcacgcca gaaatcctct tcggcttgtt gatattgtcc ctgacgttcg taagcactgg
    28141 cgcgaatcaa acttgcttgt ccacactgcg gttttttgtt cagcgcatgt gcacgtttca
    28201 gcgcctgact ggcatacgcc accgcttgcg ggaaatctgc gcggttatat tccagcatcg
    28261 ccagcgccaa gttacagcga taatccagcg ggtccagcgc cacgccgcgc aggtagtaat
    28321 cgaacggtga acggctggcg tgatgatatt gctccagatg ctgaccgata aaccaggctt
    28381 catctgtact ggtaatgtct tgtgctgcca gtggcgcttt ggcgacgtcc ggcaacggca
    28441 actcctgcgg ctgatgttcc tgataactca gtacaatatt gccgtcggca tcggagagtt
    28501 caatggtcag tctgtcagga ttgataccgt gcaacacgcc ctggatggcg gtcgctggtg
    28561 tcagggccac ggcatcatcg agcaacgcgt tgcatttgcc gatttcgcgg atcgccagac
    28621 ggtatccgtt caacggagag atggcataca gcccccactc aatcccccgc tcactacgct
    28681 ggagttttat caccgcatcg cgggaggcat tttgcaccat gcccagagaa tgataaggca
    28741 ggaaatactg ctcgaaacgc ttctcttcgt aagcatcaag ccaggtaaaa tcaggctggt
    28801 tatcggcaaa aataccggtc atcagttcga tatacgggcc gttattgtcg gtcagactct
    28861 tatcccacgc ctggccaaat tcactgtgtc cccaactcca ctgtttttta cctggcgcaa
    28921 tatggtggtt ggcaacgtgc agcaaaccgc catcttcatc gtgacaccac gcgccgacaa
    28981 aatcgtactg cgatttttcg gccatatatg aggttggaac aggcacattt ttatagcgag
    29041 aaatgtccac tccggcggag tagtccactt tgtagtaagt gccggtggcg atggggaaag
    29101 cggagacggc ccgtttgccg tgatcaaaca ccgcagtcac atccggtggg aaaacgctct
    29161 gatgcccttc accccctttc actgccgggt tgacccacca caagaaatga cgcggcgtgg
    29221 cgttgccgtt atagacgcgg ctggcgattt ccagtgccgc ccggtcaggg cgcagggtga
    29281 aacctgtcat cacctgtaaa ccatgcatcg gctccgtttc gccgacccac accgtctgtg
    29341 caccgtcttc atgggcttcg agggtgaaat caacgggcat aaaggtggtc gggcgatggt
    29401 gttgcggcca gttaaactca atcccgccgg agatccacgg tcccagcatc cccaccagcg
    29461 caggtttaat gacttcattg tgatagacaa aatcgcgctg tttcacttta tcccatgcgc
    29521 gatgcacccg accgcccagt tccggcagga tcatcacttt gatgtagtcg ttttccagcc
    29581 acaccgcctg ctaggatttc agggttttct gctcgctcag cgtatcggtc acgccgtagg
    29641 gataaaccgc gccggacgat ccctgataaa cgcgattttc caggaacatg ggatgtatat
    29701 cctgcggccc ggtttcatag gtcgggatct caacgcgctc ttgccacact tttactggag
    29761 tcatggtgcc ctcaatatta acaagacata ctgaattaaa agatttgtgg cagtgtattg
    29821 aacaatctgg caatgttttc gcggaataat cacgcaatta actaaacaag gtttagtgaa
    29881 gatgagagcc tgcattaaca atcaacagat tcgccaccat aacaaatgcg tgattctgga
    29941 actgctgtac cggcaaaagc gcgccaataa atcaacgctg gcccggctgg cgcaaatttc
    30001 gattccggca gtcagtaata ttttgcagga actggaaagc gaaaaacggg tggtgaatat
    30061 tgacgatgaa agccagacgc gcgggcatag tagcggtaca tggctgattg cgccggaagg
    30121 tgactggacg ctgtgcctga acgtgacgcc caccagtatt gagtgtcagg ttgctaatgc
    30181 ttgtttaagt ccgaaaggcg aatttgagta tttacagatt gatgtaccga cgccgcaggc
    30241 gctgctgtcc gaaatcgaaa aatgctggca tcgccaccgt aaattgtggc cggaccgcac
    30301 catcaatctg gcgctggcaa tccacgggca ggttgatccg gtgaccggcg tatcgcaaac
    30361 catgccgcaa gcgccgtgga caacgccagt tgaggtgaag tatctgctgg aagagaagct
    30421 gggcattcgg gtgctggtcg ataatgactg cgtgatgctg gcgctggcgg agaaatggca
    30481 aaataattcg caggaacggg atttctgcgt gatcaacgtt gattacggca ttggctcgtc
    30541 gttcgtgatt aacgagcaaa tttatcgcgg cagtttgtat ggtagcggac agattggtca
    30601 caccatcgtt aatccggatg gagtcgtctg cgactgtgga cgttacggca gcctggaaac
    30661 cgtcgcctcg ttaagtgcat taaaaaaaca ggcgcgggta tggctaaaat cacaaccggt
    30721 taatactcag cttgagcctg aaaaactgac aaccgcgcag ttaatcgctg cctggcagag
    30781 tggagaaccg tggatcacca gctgggttga tcgctctgcc aatgccattg gtttgagtct
    30841 gtataacttc ctcaacatcc tcaatattaa tcagatttgg ttgtacggtc gcagttgtgc
    30901 ctttggtgag aactggctta atactattat tcgccagaca ggatttaacc cgttcgaccg
    30961 cgacgaagga ccgagcgtga aagcgacgca aattggcttc gggcaattaa gccgcgcaca
    31021 acaggtgctg ggaattggct atttgtatgt tgaggcgcag ttacgacaga tttgatggcg
    31081 cgataacgta gaaaggcttc ccgaaggaag ccttgatgat cataaacgaa aaattgcctg
    31141 atgcgctacg cttatcaggc ctacacggag attgcaatat attgaatttg caagattttg
    31201 taggcctgag aaggtgttca tgccgcatcc aacatgaaca acgagcacat tgtcagcaaa
    31261 tcaccgtttc gcttatgcgt aaaccgggta acgtgcgcag atgtcgagaa ctttaccttt
    31321 gatgcgctcg ataacggctt catcattgat gctgtccagc acgtcacaca tccagccagc
    31381 cagttctttc gcttcggctt ctttaaagcc gcgacgggta atcgccggag tacctacacg
    31441 aataccggag gtcacaaacg ggctcttcgg atcgttcggt acgctgtttt tgttgacggt
    31501 gatgttagca cggcccagag cggcgtctgc ttctttaccg gtcaggtttt tatcaaccag
    31561 atcaaccagg aacaggtggt tatcagtgcc gccggaaacc actttgtagc cgcgctcgag
    31621 gaacacttct accatcgctt tagcgttttt cgcgacctgc tgctggtaag ttttgaactc
    31681 aggctccatc gcttctttca gagcaaccgc tttaccggcg attacgtgca tcaacggacc
    31741 gccctgacca ccagggaaaa cggcagagtt cagttttttg tacagctctt cgctaccacc
    31801 tttcgccaag atcaggccgc cgcgcggacc cgccagggtt ttgtgagtgg tggtagtaac
    31861 aacgtgagca tgaggaaccg ggttcgggta gacgccagca gcaaccaggc ccgcaacgtg
    31921 cgccatatca acgaacagat aagcaccgat gctgtcagcg atttcacgca ttttcgccca
    31981 gtcaaccacg ccggaatatg cagagaaacc accgataatc attttcggct tgtgttcttt
    32041 ggcttgtttt tccagatcgg cgtaatcgat atgaccggta gcatcgatac cgtaaggaac
    32101 gatgttgtac agtttaccgg agaagttaac cggagaaccg tgagtcaggt gaccgccatg
    32161 cgccaggttc atacccagaa cggtatcacc tggttccagc agcgcggtgt agaccgcaaa
    32221 gttagcctgg gagccggagt gcggctggac gttagcgtag tcagcgccga acagttcttt
    32281 cgcacgatcg atcgccagtt gttcaacgat atcaacatac tcgcaaccgc cgtagtagcg
    32341 tttgcccgga taaccttcag catatttgtt ggtcagctga gaaccctgcg cctgcattac
    32401 gcgcgggctg gtgtagtttt cggaggcgat cagttcgatg tgctcttcct gacgtacttt
    32461 ttcctgctcc atagcctgcc acagttcggc atcataatcg gcaatgttca tttcacgctt
    32521 taacatccgc atctcctgac tcagctaaca ataaaatttt tggcctttat aggcggtcct
    32581 gttggacaac ggcgaacagt ataaccgaat cattgtgcga taacaggtct tgacaaagga
    32641 atttacgcaa acgattacct tcaggctacg caaggctttg gagaataaag agcttgcaac
    32701 cggaaacgga tttcttttca ggtttgtgat gcaaattttt cacttcatca cattctttct
    32761 gaaaaacacc aaagaaccat ttacattgca gggctatttt ttataagatg catttgagat
    32821 acatcaatta agatgcaaaa aaggaagacc atatgcttga cgctcaaacc atcgctacag
    32881 taaaagccac catcccttta ctggtggaaa cggggccaaa gttaaccgcc catttctacg
    32941 accgtatgtt tactcataac ccagaactca aagaaatttt taacatgagt aaccagcgta
    33001 atggcgatca acgtgaagcc ctgtttaacg ctattgccgc ctacgccagt aatattgaaa
    33061 acctgcctgc gctgctgcca gcggtagaaa aaatcgcgca gaagcacacc agcttccaga
    33121 tcaaaccgga acagtacaac atcgtcggtg aacacctgtt ggcaacgctg gacgaaatgt
    33181 tcagcccggg gcaggaagtg ctggacgcgt ggggtaaagc ctatggtgta ctggctaatg
    33241 tatttatcaa tcgcgaggcg gaaatctata acgaaaacgc cagcaaagcc ggtggttggg
    33301 aaggtactcg cgatttccgc attgtggcta aaacaccgcg cagcgcgctt atcaccagct
    33361 tcgaactgga gccggtcgac ggtggcgcag tggcagaata ccgtccgggg caatatctcg
    33421 gcgtctggct gaagccggaa ggtttcccgc atcaggaaat tcgtcagtac tctttgactc
    33481 gcaaaccgga tggcaaaggc tatcgtatcg cggtgaaacg cgaagagggt gggcaggtat
    33541 ccaactggtt gcacaatcac gccaatgttg gcgatgtcgt gaaactggtc gctccggcag
    33601 gtgatttctt tatggctgtc gcagatgaca caccagtgac gttaatctct accggtgttg
    33661 gtcaaacgcc aatgctggca atgctcgaca cgctggcaaa agcaggccac acagcacaag
    33721 tgaactggtt ccatgcggca gaaaatggcg aagttcacgc ctttgctgat gaagttaagg
    33781 aactggggca gtcactgccg cgctttaccg cgcacacctg gtatcgtcag ccgagcgaag
    33841 ccgatcgcgc taaaggtcag tttgatagcg aaggtctgat ggatttgagc aaactggaag
    33901 gtgcgttcag cgatccgaca atgcagttct atctctgcgg cccggttggc ttcatgcagt
    33961 ttaccgcgaa acagttagtg gatctgggcg tgaagcagga aaacattcat tacgaatgct
    34021 ttggcccgca taaggtgctg taatttgatg ttgccggatg gaaacatccg gcaacccttg
    34081 acgcggttta aattgccgcg tcgtcctctt caccggtacg gatgcgaatg acccgagcca
    34141 cgtcaaagac gaagatttta ccgtcaccga ttttgccggt ttgcgccgtg cgaataatgg
    34201 tatcgacaca ggtatcgaca atgtcgtccg gtacgacaat ctcaattttc actttcggca
    34261 gaaaatccac catatactcc gcgccgcggt acagctcggt atggcctttc tggcgaccaa
    34321 agcctttcac ttcggtcacc gtcatgccgg taataccgac ttcggccagt gcttcgcgga
    34381 catcgtccag cttgaagggt tttataatcg catcaatctt tttcatgcta ttccttgaaa
    34441 aggttgcctg tcttttgatc tgctaaacgt aacacataac gccaattcat tccttgaaat
    34501 cgtttgcatc cagctcgtgt cgggaaagca gtttataaaa ttctgtccgg ttgcgccccg
    34561 ccattctcgc cgcgtgggtg acgttgcctt tggtgatttg cagcagctta cgcaagtagt
    34621 tgagttcaaa atgattacgt gcctcaacaa aggttggcag cgccgtattt tcaccctcca
    34681 gcgcctgctc caccagcgca tcactaatca ccggagatga ggtcagcgcc acgcactgtt
    34741 caatcacgtt gaccaactgg cgcacattac ccggccagct cgcggtcatc aggcgtttca
    34801 tcgcatcggt agagaacgcg cggacaaacg gtttatgtcg ctctgccgcc tggcgcaaca
    34861 ggtgatttgc cagtagcgga atgtcttctg tgcgctccgc cagtgccgga attttcaggc
    34921 tgacaacgtt gaggcggtaa tagagatctt cgcggaattc cccgcgcgcc atcgcttttg
    34981 acagatcacg gtgagtggca gaaataatcc gcacattgat atcaatatcg cggttactgc
    35041 ccagcgggcg cactttacgc tcctgcaaca cgcgcagcag tttgacctgt aacggcgcag
    35101 gcatatcgcc aatttcatcg agaaatagcg ttccgccttc cgccgcctgg aataaacctt
    35161 cgcgattgct gacagcgcca gtaaacgcgc cacgcgcatg accaaacagc tccgactcca
    35221 gcaattgctc gggcaatgcg ccacagttaa tagcaataaa tggtttgctg ttgcgcgggc
    35281 tggcgttgtg gatagcctgg gcgaaaatct ctttcccggt gccgctctga ccgttaatca
    35341 aaacgctgac gtctgattgc gccaccagcc gcgcctgttc cagcaaacgc agcatcagcg
    35401 ggctgcgggt gacaattgcc tcgcgccagc gttcatcggt ggctggcgcg gattgctcca
    35461 gcgcatcgtc aattgcctga tatagcgcat ctttatcgac aggcttggtg aggaaactaa
    35521 aaacgccctg ctgtgttgca gcaacggcat cggggataga accatgcgca gtaagaataa
    35581 ttactggcat tcccggctgc actttctgga tttcagcaaa tagctgcata ccgtccattt
    35641 catccatccg caggtcgctg ataactaaat ctactttttc gcgattcagt acccgtaatc
    35701 cttcagcgcc actttccgcc gtgaccacac tgtagccttc gctggtcagg cgcaggccaa
    35761 gcagtttcag caatcccgga tcgtcatcga ccaataataa atgcgcaggt ttatggctca
    35821 tcaggagtga cctcatgggt ggatggcgcg ggcttttcac tctcgtgcgg cgtatccggc
    35881 gagaaatttc cagccggttt gcgggtcgag agctggcgtt caatatcggt caggttttcc
    35941 agcttgcggg tggtgagttc cagttgctgc tgtagaacgt gatgttgctg gcgcaatgta
    36001 tccagctcgc tgtcgctgga ctgctggagt ttactgtaac gttggcgctc ttccgccagt
    36061 tgcagttgca gcgcctgacc gtcgcgccag agttgataca gtgggcgaac ctgtgccggg
    36121 atctcggtac ttaacgcttc aatacgcgcg accagctggc ggcgctcata cggcgtaatt
    36181 ttggcgtcgg cgagcaaaat cccttgttta aaggtatttt gccagctgcc gtcgtcatat
    36241 tggcgagctt gctgacgcga ctgcgcaggc attaaacgat cagcacaatc catcgcccgc
    36301 agccagtaaa gcggattggt ttcggttgat ttaccttgca gcgcccagat gtcgctacat
    36361 tcagtagaaa gatagtcagc cagttgataa accggaattt tttcttctgc tggcgtatca
    36421 atggctggct tattgtgatt ctgcacgcaa cccagcaatg ccagacatgg cagccctgcc
    36481 agccacagcc gtcggggcaa taatcgttga aaaatgtgtc gcatattcac cagacttaaa
    36541 gcctatccca gtgggcgtaa ttgttgcaga cagtctggac atggacagcg cggagaaacc
    36601 ggggcgtaca tatcgtacgt gaagatttcg agcactaccc ggggccgaac tgacaaataa
    36661 aatagcctga taggataggc tgttagcatt atttcgtgtt tttcgacgac ggtaattcaa
    36721 tgcggaaaca aacgtcttgc ccgctctcgt cgaccagata cagttcccct tgcatacggc
    36781 gaatacaatc cctggcaatg cttaatccca gaccgctgcc cttcaccgcc ccttttcgct
    36841 ggtggcttcc ctgaaaaaag ggttcgaaga tcatggcgcg ttcctcttgc ggaatgggcg
    36901 tgcctgtatt gatgacatca atataaaccc gcgcaccatg taaactgctg cgaaggcaaa
    36961 tgttaccgga ttcagccccg tagtgcaccg cattggagta aagattatcc agtacgctca
    37021 tcagcagcat tggctccgcc aggcaagctg ttgctttgag atcgacgtcg gtatgcatca
    37081 ttttagcccg tgcgggcagg ctatgagcag aaaccactgt ctccaccagc ggtgctaatt
    37141 caacattctc cagttccacc gcactgtccg cctgtttacg gttgtaatca agcagttgtt
    37201 cgatcagttt ttgcaaattg cagctgctgc tatcaagaat gctcaccacc tctttttgct
    37261 ctggcgtaag cggcccgaca acctgatcag ccagtaattc agtgccctcg cgcatactcg
    37321 ccagtggcgt ttttaattca tgagataaat gtcttaaaaa ttgatggcgt tgggattcca
    37381 gccatgacag gcgctcactt aaccaaagaa tacgttgccc aaccgagcgt aactcgctcg
    37441 gtccacagaa cgaaacgcta ttgcccagag aacgcccttc ccccagacgg ttgatcatgc
    37501 gctcgatatt tttcaccggc ccgataatca tccgcgtgaa aagtagcacc atcaccagac
    37561 tcaccagaaa tagcaccagc gattgccaac caaaatattg cccacgttcg gcgatttcac
    37621 gctgaagttg ctgcccacga gagaacacca ctgtgcgcgt ggcctgcacc atttcggtat
    37681 tggcactggc aaaggcttca agacgcgcgg cggcggcagc atcgggacca ctgttgttac
    37741 actgaagttg ggccagattg ttcaaatcct gacgcaatgc ctggtaaagt ttatcgtctg
    37801 gcagtacgcc tgcgtgggca tcgagcattt cgctgtaacg cttgcgctgg ctttgataaa
    37861 ccttcgcaag cgtagggtcg tccagcacgc aatactgacg gtagctacgc tccatctcca
    37921 gcgccgcgtt ggtcatcgct tcactacgcc gggcatcaat aagcgtagtg cggttaacca
    37981 gcgccgcctg atcgctaagc gcattcaggc tttgccaggc ttgccatgcc agcaccaaca
    38041 ggggtagtag aattagcaaa aatgccagca ttaccagttg tcgtaatgag cggggaaaaa
    38101 cgggccagcg tttcaaggtg ttactctcgt cagacgcgaa tagcctgatg ctaaccgagg
    38161 ggaagttcag atacaacaaa gccgggaatt acccggcttt gttatggaat aaggcggtgc
    38221 ctaactcgac gtttcgcccg atggttgata tagctacgct gatatcagaa gttggacggc
    38281 aggcaccttg ttgtgcgtca ttcgaatttt atgtagcacg tcccgaaggg gctgacataa
    38341 gtcggtgaat gagccactgc ttactattat gcagtaactg tgccaataaa gaaaatagtt
    38401 tggtaacgta ctgattatac gttgctttga gggatttatg gcttcttcgc tatttgaatg
    38461 taaatcagcc actgtgtcgc taaaaagaga caacttaaga taaacttatt agattacaat
    38521 aaaatcaatc atttaagtgt cgccagaaag cgacacggta aaccacccat tgtcgtgatt
    38581 tacagacaca aaaaagctcc cggagttggg agcttatgat agtggttggt gcttaatgcc
    38641 gcatccgggc tgcaaaacca atgaactgat gacttacccc aactgcttac gcgcattgcg
    38701 gaaaatgcgc atccatgggc catcctcgcc ccagttttcc ggatgccagg agttgctgac
    38761 agtacggaaa acacgttccg ggtgcggcat cataatggtg actcgaccac tttcagtcgt
    38821 aaccgccgta ataccgttcg gtgaaccgtt cgggttagcc gggtaggttt cagtgacttt
    38881 gccgaagtta tcgacatagc gcagtgccac cagccctttg ctttccagtg ccgccagatg
    38941 cgccgcatca cgcacttcca cgcgcccttc accatgagag acagcaatcg gcatttgcga
    39001 gcccaccatc ccctgcaaca gcagagacgg gctttgggtt acttcaacca ggctgaaacg
    39061 cgcttcaaag cgatcggagg tgttacgcac aaaacgtggc cacaactcac tccccgggat
    39121 cagttcacgc aggttagaca tcatctggca accgttacat acccccaggg ccagcgtttg
    39181 cggacgatgg aagaaggttg caaactcatc gcgtacacgg tcattgaaca ggatagactt
    39241 cgcccaacct tcaccggcac ccagcacatc accgtaggag aaacctccgc acgcgaccag
    39301 ggcgtgaaaa tcctccagtc ccgtacgtcc agtcagcagg tcgctcatat gcacgtcgat
    39361 agcatcaaag cccccacgtt ggaaagctgc cgccatttca acatgcgagt tcacgccctg
    39421 ctcacgcagt acggcaactt tcggacgtgc gccagtggca atatacggtg ctgccacatc
    39481 ttcgttgata tcgaacgaca gttttacatt caggcccgga tcggcgtcgt tagatttcgc
    39541 ctgatgctcc tgatcggcac actccgggtt gtcacgcagg cgctgcatct gccaggtagt
    39601 ttctgcccac cagacacgca acgtagtgcg gctttcgctg aatacagtct gcccgttggc
    39661 ggtaatcaca aaacggtcac cggaaaccgc ctgcccaaca taatggacgc aatccgcaag
    39721 cccatgctgt gccagtacgg actcgaccgc ttcacggtca gcggcacgaa cctgaatcac
    39781 cgcacccagt tcttcgttaa acaacgccgc caggcgatcg tcacccagag aggcgatatc
    39841 cgcgtcaatg ccacaatgac ctgcaaaggc catttccgcc agcgttacca gcaggcctcc
    39901 atcagagcgg tcgtgatacg ccagcagctt acgctgtgca accagcgcct gaatcgcgtc
    39961 atagaagcct ttcagttgga caacatcgcg tacatctgcc ggtttgtcgc caagctgacg
    40021 ataaacctgc gccagcgccg ttgcgcccag cgcgttattg cctttgccca aatcaatcag
    40081 cagcagtgcg ttatcttcgg tacaaagctg cggcgtgatg gtgtgacgta catcttccac
    40141 gcgggcaaat gcagaaatca ccagcgacag cggcgacgtc atttcgcgct cttcgttacc
    40201 ttcctgccag cgggttttca tcgacataga gtctttaccc accgggatcg tcaggcccag
    40261 cgccggacaa agttcttcac ccacggcttt aacggcttca tacaggcccg catcttcacc
    40321 agggtggcct gccgccgcca tccagttggc ggaaagttta atgcgtttga tatcgccaat
    40381 ctgtgttgcg gcaatattgg ttaacgcttc accgaccgcc agacgggcag aggcggcgaa
    40441 atccagcagc gcaaccggcg cacgctcgcc aatcgccatc gcttcaccgt agtagctgtc
    40501 gaggctggcg gtagtgaccg cgcagttagc gaccggcacc tgccacggcc ccaccatctg
    40561 atcgcgcgcc accatgccgg ttacgctgcg gtcaccgatg gtcaccagga aagttttctc
    40621 cgccacggtc ggcagatgca gtacgcgttt caccgcgtct gcaatggtga tcccttcacg
    40681 gaccagcgca tcgcccttcg ctttcagcgt ttgtacatcg cgggtcatct tcggcgtttt
    40741 accaagcagg acgtccagcg gcagatcgat cggctgatta tcaaaatgac gatcgtgcag
    40801 agaaagatgc agttcttcgg tcgcttcacc aatcaccgcg tagggtgcgc gctcacgctt
    40861 acacagttcg tcaaacagcg gtaattgatc ggcagcaacc gccagcacgt agcgttcctg
    40921 ggattcgtta caccagattt ccagcgggct catgcccggt tcgtcgctta gaatatcgcg
    40981 cagttcaaat ttaccgccgc gtccgccgtc gctcaccagc tccggcatcg cgttagaaag
    41041 accgcccgca ccgacgtcgt ggataaacag aattgggtta gcatcaccaa gctgccagca
    41101 acggtcgatc acttcctggc aacgacgctc catctccggg ttgtcgcgct gtacggaagc
    41161 aaagtcgagg tcggcatcag actgaccaga cgccatggaa gacgccgcgc cgccgccaag
    41221 accgatgttc attgccgggc cgccgagaac gaccagcttc gcaccgacgt tgatctcgcc
    41281 tttttgtacg tgatcggcgc gaatgttgcc gatcccgccc gccagcatga tcggtttgtg
    41341 ataaccgcgc agctcttcgc cattgtggct gttcactttt tcttcataag tacggaagta
    41401 gccgttcagt gccggacgac caaattcgtt gttaaacgcc gcaccgccca gcgggccttc
    41461 ggtcatgatg tccagcgcgg taacaatgcg ctcaggctta ccgaaatctt cttcccacgg
    41521 ctgttcgaag ccaggaattc gcaggttgga tacggagaaa ccaaccaaac cagctttcgg
    41581 cttcgcgccg cgcccggtgg caccttcatc gcggatttca ccgccggaac cggtcgccgc
    41641 gcccggccac ggagaaatcg ccgtcgggtg gttgtgagtt tcgactttca tcaggatgtg
    41701 cgccggttcc tgatggaaat tgtagcggcc cgtttcgtgg tcagcaaagt agcggcccac
    41761 ttcagaacct tccattacgg cggcgttatc tttataagca gagagaacgt gatctggcgt
    41821 ggtttcgaaa gtatttttga tcatcctgaa cagcgatttc ggctgttgtt caccgtcgat
    41881 aatccagtcg gcgttaaaaa ttttgtggcg gcagtgctca gagttcgcct gagcgaacat
    41941 atacagctcg atgtcgttcg ggttacgacc aagctttgtg aaagcatcct gcagatagtc
    42001 aatttcatct tccgccagag ccaagccaag acgcaggtta gcgtcgatca gcgcctgacg
    42061 gccctgcccc agcaaatcaa cgcttgtgac cggagtcggt tgatggtggg caaacaactg
    42121 ctctgcatca tctaaatcaa aaaagaccgt ttccatcatg cggtcgtgca gttcagcggt
    42181 aacctgctgc cattgttcat tggtcagcgt accggcttct atatagtaag caacgccgcg
    42241 ctcaaggcgg tttacctgtt gtagcccgca gttatgggca atatcggtcg ctttcgaaga
    42301 ccagggagag atggtgccag gacgcggggt caccagcagg agtttgcctt gcggggcgtg
    42361 gctggcgagt gccggaccat atttcagcag gcgttcaagt tgtgcgtgct catcatcgtt
    42421 taacggcgca ttgaggtcag caaaatggac atactcggcg taaatattgt gaaccgggag
    42481 cctggcagcc tgaaaacgtg ccagcagttt gttgattcgg aatgccgaca gtgcaggcga
    42541 accacgcaga atttccatca taagtctctc gtcttctaag ctttcggtgt acccaagggg
    42601 ggggaaacgg gcgtcattat aaagaatctg atgcgctgac gaaaccgttt gcgtggaaat
    42661 aaaatcacca tcgtgaatta gcaacgcgtg ccgccaatgg ctgtaataag ttgccatctg
    42721 gcgcaggttt acgcaaaatg ccgctcattt atgagtaaac ctttcactat tattacgttt
    42781 tttcaagctg ggacgcgcac gacacagaga attaactaat tgaaaaaatt aaagattaat
    42841 tatctgttca tcggcattct ggcactgctg ctcgcggtcg ctctctggcc atccattccc
    42901 tggtttggta aagccgacaa ccgtatcgcc gccattcaag cgcggggaga gttgcgtgtg
    42961 agcaccattc atactcccct gacttataac gaaatcaacg ggaaaccttt tggcctggat
    43021 tacgaactgg cgaaacagtt tgccgattac ctcggcgtaa aactgaaagt gaccgtgcgg
    43081 cagaatatca gccagctgtt tgacgacctt gataatggta acgccgacct gctggcggca
    43141 ggactggtct ataacagtga gcgggtaaaa aattatcagc ctggccctac ctattattcc
    43201 gtgtcacaac aactggttta taaagtgggt cagtatcgcc cacgtacgct gggcaacctg
    43261 acggcggagc aactcaccgt tgcaccgggt catgtggtag ttaacgatct ccagaccctg
    43321 aaagaaacaa aattcccgga attaagctgg aaggtagacg acaaaaaagg ctctgcggaa
    43381 ttaatggaag atgtcatcga aggaaaactc gattacacca ttgctgattc tgtcgccatc
    43441 agcctgtttc agcgcgttca cccggagctc gccgtagcgc tcgatatcac cgatgaacaa
    43501 ccggtgactt ggtttagccc gttagatggc gataataccc tttccgccgc cctgctcgac
    43561 ttcttcaacg aaatgaatga agacggtacg ctggcacgca ttgaagagaa atacctgggg
    43621 catggcgatg attttgatta cgtcgatacg cgcacatttt tacgcgccgt cgatgcggta
    43681 ctgccgcagt taaagcccct gtttgagaaa tacgccgaag aaattgactg gcgtttgctg
    43741 gccgctattg cttatcagga atcgcactgg gatgcacagg ccacttcacc gacgggtgtg
    43801 cgcggcatga tgatgttaac caaaaatacc gcgcaaagcc tcggcattac ggatcgtacc
    43861 gatgccgaac agagcatcag cggtggcgtg cgttatttgc aggatatgat gagtaaagtg
    43921 ccggaaagtg tgccggagaa cgaacggatc tggtttgccc tcgctgcgta caatatgggc
    43981 tatgcgcata tgctggatgc ccgcgccctg acggcaaaaa ccaaagggaa tcctgacagt
    44041 tgggctgacg taaaacagcg tctgccttta cttagccaga aaccctatta cagcaagctg
    44101 acttacggct acgctcgtgg gcatgaagcc tacgcttatg tcgaaaatat tcgtaagtat
    44161 cagattagcc tggtgggtta tctgcaagag aaagagaagc aggctacaga agcggcgatg
    44221 caactggcgc aggattatcc ggcggtatcg cctacggagt tgggcaaaga gaaatttcct
    44281 tttctctcgt ttctttccca gtcgtcatca aactatttga cccattctcc ctctctgctg
    44341 ttttccagga aagggagtga agagaaacaa aattaatccg tcgaggattg cgcttttttc
    44401 tgcgctttaa tttcctggcg gcgcatgcga aagaagtcac tgagcaacgc cgcgcactca
    44461 tccgccagta ttccttccgt aatttccact cggtgattca tacccggatg atgcagcaca
    44521 tccattaaag atcccgcagc gccagttttc gcgtcacgcg caccaaagac cacgcgacca
    44581 atgcgactgt ggatcatcgc tccggcacac attacacatg gttcaagcgt gacatacaac
    44641 gtggcgtcga tcagacgata attttgcatc accagaccac cctgccgcag ggccatgatt
    44701 tctgcatgtg cggtgggatc atggcgacca atcgggcggt tccagccttc gccgattacc
    44761 cgattgttat gcactaatac cgcgccgacc ggcacttccc gctcatccca ggcacgtttc
    44821 gccagcgtca gcgcgtgacg catccaatat tcgtggctaa attcgacttc agacaaaaag
    44881 aaaactccgg ttataaaagc gcggcgcatt atacacggac gctatgcttt actctattcc
    44941 agttgctgga gttcaccgcg cggggttact cgccaacgat gctgacagaa ataaagcagc
    45001 ggattgtcct gattactgtc gctatagcca ctgtacagcc gcagcggcgt gccgattttg
    45061 cgctccagtt gtgcgacctt ttcatgtccc agacaacgca tcgtcaatac ccaaccacca
    45121 tagccacgct gaatttggct ggcgataaga ttaacccgcg gcagccaggg cgtatcgaaa
    45181 taaaccgctt caaccagcgg ctgcggagag ccggtaatca accagatatc agcatcggaa
    45241 cttaacaggt aggtggttaa tcgctcctga accagcggaa aggcggtaac attgtcgcga
    45301 aaccagcgca cgaaatcggc ctgcaacgtc tgtaaacgtg cttcgctgtg accaaaagtg
    45361 cacccccaca gaagcagact catcggccag cgtgccgcac gaccttttat caataacgca
    45421 atggctataa tcggtaacaa cggcaggaca agtaacgcat tcagcggttg gcgacgcagt
    45481 aaatagcgca gaaaactgcc gaacatatcc tgctgatgca atgttccatc taagtcaaaa
    45541 aacaccacac gacgctcgtg agttgccaaa ccatactcct ctggactgaa acatcctgat
    45601 taattttcgc tactacatag cctaacagat agatcatcac ttttccggca acaatcgacc
    45661 gcatcatggc taactggaat ttttaattca tggcaattag cggcaatgga atataaaatt
    45721 cactcgcgtg tgtctcatat tgcctgttgt cgccactatt tcatcgcgag gaaagatatg
    45781 aacggcttac ttcgtatccg tcagcgttac caagggcttg cccagagtga taaaaaactg
    45841 gcggattatc tgctgctaca acctgatacg gcgcgccatt taagctctca gcaactggcc
    45901 aacgaagccg gagtcagtca gtccagcgtc gtgaagttcg cgcaaaaact cggctataaa
    45961 ggttttccgg cgcttaagtt ggcgttgagt gaagcgctgg caagccagcc ggaatcgccc
    46021 tccgtgccca ttcataacca aatccgcggt gatgatccgt tacggctggt cggcgaaaaa
    46081 ctgattaaag aaaataccgc cgctatgtac gcaacgctaa acgttaatag tgaagagaaa
    46141 ctgcatgaat gcgtaacaat gttgcgctct gcgcggcgga taattctgac cggtattggc
    46201 gcttcgggtc tggtggcgca aaactttgcc tggaagctga tgaagattgg cttcaatgct
    46261 gccgcactgc gcgatatgca tgcgctactc gcaacagtac aggcgtcgtc ccctgacgat
    46321 ctgttattag ccatttccta caccggtgta cgacgcgagt taaacctggc ggcagatgag
    46381 atgctgcgag tgggcggaaa agtgctggcg attaccggct ttactccgaa tgccctgcaa
    46441 cagcgtgctt ctcattgcct gtataccatt gccgaagaac aagcgacaaa cagtgcttca
    46501 atctctgctt gtcacgctca gggaatgtta acggatttgc tgttcattgc gctgattcag
    46561 caggatctgg aactggcacc agaacgtatt cgtcatagtg aagcgctggt gaaaaaactg
    46621 gtctgagtaa agaatgcgcg tataatgccc gcccggtttg tgttgttttg agagtttcct
    46681 tatggcgctg ttaatcacta aaaaatgcat caattgtgat atgtgtgaaa tcaaataaaa
    46741 acacatttat tttcatagtg atacagcaat gtgttattga atgtgttgtt ggatttatgg
    46801 tgcaaaaaaa ttttgcataa gttcagcaac acaaagaaca ccactttctt taacgtcctc
    46861 cgcgccctaa aggacggggc tttacggcgc aaaaggcctg tgacatacaa cattaacttt
    46921 tcagtcaacc gtagcacgtt cttgtatacg ccgtgtctgc ggttttatat aaccctggct
    46981 ggaaacctct tatacaaagt cgattcgcca acatcataga tgatcgccac cttctggcga
    47041 gaaactcctg atgcaattag tcgcccggcc tgcgcccatt attctggtga aagtttggga
    47101 cggcgaccgc caattcgtcc ctgtgcgcga gcagcttcca gtcctgcttt tgttcgttca
    47161 acaatcagtt cacgttccat ttcagccagg gcacccatca catggaagaa aaagcgtccc
    47221 attggtgtgc tggtatcaat tgaatcagtc agactacgaa agttgatgcc tcgttcgcgc
    47281 aacgcctcca ccagcacgac aagatgccgc atactgcgcc ccagccgatc cagcttccag
    47341 acaaccagtg tgtcgcctgc cgataatgtc ttgagcagtt ttttcagccc cggtctttcg
    47401 gactttgtac cacttatctt gtcttcaaaa atcagctcgc atcctgcaca gttcaacgca
    47461 ttacgttgta gatcggtatt ctggtcattt gttgatacgc gtacatagcc aa
//
LOCUS       DAETQL010000027        46125 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.027, whole genome shotgun sequence.
ACCESSION   DAETQL010000027 DAETQL010000000
VERSION     DAETQL010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.027"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            95..2248
                     /locus_tag="K0W65_001593"
     CDS             95..2248
                     /locus_tag="K0W65_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBI3094896.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..4031
                     /locus_tag="K0W65_001594"
     CDS             2301..4031
                     /locus_tag="K0W65_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094897.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="K0W65_001595"
     CDS             complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="K0W65_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBI3094898.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4750..5661
                     /gene="panE"
                     /locus_tag="K0W65_001596"
     CDS             4750..5661
                     /gene="panE"
                     /locus_tag="K0W65_001596"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBI3094899.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5624..6214
                     /gene="yajL"
                     /locus_tag="K0W65_001597"
     CDS             5624..6214
                     /gene="yajL"
                     /locus_tag="K0W65_001597"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBI3094900.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="K0W65_001598"
     CDS             complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="K0W65_001598"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBI3094901.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQAAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7922..8164
                     /gene="xseB"
                     /locus_tag="K0W65_001599"
     CDS             7922..8164
                     /gene="xseB"
                     /locus_tag="K0W65_001599"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBI3094902.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8164..9063
                     /gene="ispA"
                     /locus_tag="K0W65_001600"
     CDS             8164..9063
                     /gene="ispA"
                     /locus_tag="K0W65_001600"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBI3094903.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9088..10950
                     /gene="dxs"
                     /locus_tag="K0W65_001601"
     CDS             9088..10950
                     /gene="dxs"
                     /locus_tag="K0W65_001601"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBI3094904.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11005..11922
                     /gene="yajO"
                     /locus_tag="K0W65_001602"
     CDS             11005..11922
                     /gene="yajO"
                     /locus_tag="K0W65_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBI3094905.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="K0W65_001603"
     CDS             complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="K0W65_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBI3094906.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="K0W65_001604"
     CDS             complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="K0W65_001604"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBI3094907.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="K0W65_001605"
     CDS             complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="K0W65_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBI3094908.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="K0W65_001606"
     CDS             complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="K0W65_001606"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBI3094909.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="K0W65_001607"
     CDS             complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="K0W65_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBI3094910.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="K0W65_001608"
     CDS             complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="K0W65_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBI3094911.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16289..16828
                     /locus_tag="K0W65_001609"
     CDS             16289..16828
                     /locus_tag="K0W65_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBI3094912.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17126..18010
                     /gene="tsx"
                     /locus_tag="K0W65_001610"
     CDS             17126..18010
                     /gene="tsx"
                     /locus_tag="K0W65_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBI3094913.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18187..18534)
                     /locus_tag="K0W65_001611"
     CDS             complement(18187..18534)
                     /locus_tag="K0W65_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBI3094914.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18663..19634)
                     /gene="secF"
                     /locus_tag="K0W65_001612"
     CDS             complement(18663..19634)
                     /gene="secF"
                     /locus_tag="K0W65_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBI3094915.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19645..21492)
                     /gene="secD"
                     /locus_tag="K0W65_001613"
     CDS             complement(19645..21492)
                     /gene="secD"
                     /locus_tag="K0W65_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBI3094916.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="K0W65_001614"
     CDS             complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="K0W65_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBI3094917.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="K0W65_001615"
     CDS             complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="K0W65_001615"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBI3094918.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23057..24127)
                     /gene="queA"
                     /locus_tag="K0W65_001616"
     CDS             complement(23057..24127)
                     /gene="queA"
                     /locus_tag="K0W65_001616"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBI3094919.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24220..24793
                     /gene="acpH"
                     /locus_tag="K0W65_001617"
                     /pseudo
     CDS             24220..24793
                     /gene="acpH"
                     /locus_tag="K0W65_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="K0W65_001618"
     CDS             complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="K0W65_001618"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBI3094920.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26771..28144)
                     /gene="proY"
                     /locus_tag="K0W65_001619"
     CDS             complement(26771..28144)
                     /gene="proY"
                     /locus_tag="K0W65_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBI3094921.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="K0W65_001620"
     CDS             complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="K0W65_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBI3094922.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="K0W65_001621"
     CDS             complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="K0W65_001621"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBI3094923.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="K0W65_001622"
     CDS             complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="K0W65_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HBI3094924.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32178..33380
                     /gene="sbcD"
                     /locus_tag="K0W65_001623"
     CDS             32178..33380
                     /gene="sbcD"
                     /locus_tag="K0W65_001623"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBI3094925.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33377..36520
                     /gene="sbcC"
                     /locus_tag="K0W65_001624"
     CDS             33377..36520
                     /gene="sbcC"
                     /locus_tag="K0W65_001624"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBI3094926.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36646..37827
                     /gene="araJ"
                     /locus_tag="K0W65_001625"
     CDS             36646..37827
                     /gene="araJ"
                     /locus_tag="K0W65_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBI3094927.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37970..38878)
                     /gene="mak"
                     /locus_tag="K0W65_001626"
     CDS             complement(37970..38878)
                     /gene="mak"
                     /locus_tag="K0W65_001626"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBI3094928.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39003..39914
                     /gene="rdgC"
                     /locus_tag="K0W65_001627"
     CDS             39003..39914
                     /gene="rdgC"
                     /locus_tag="K0W65_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBI3094929.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39992..40447)
                     /locus_tag="K0W65_001628"
     CDS             complement(39992..40447)
                     /locus_tag="K0W65_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094930.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40444..40779)
                     /locus_tag="K0W65_001629"
                     /pseudo
     CDS             complement(40444..40779)
                     /locus_tag="K0W65_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="K0W65_001630"
     CDS             complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="K0W65_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBI3094931.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41290..41967)
                     /locus_tag="K0W65_001631"
     CDS             complement(41290..41967)
                     /locus_tag="K0W65_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBI3094932.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="K0W65_001632"
     CDS             complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="K0W65_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBI3094933.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="K0W65_001633"
     CDS             complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="K0W65_001633"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBI3094934.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43173..43631)
                     /locus_tag="K0W65_001634"
     CDS             complement(43173..43631)
                     /locus_tag="K0W65_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBI3094935.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RECLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43751..44560
                     /gene="proC"
                     /locus_tag="K0W65_001635"
     CDS             43751..44560
                     /gene="proC"
                     /locus_tag="K0W65_001635"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBI3094936.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="K0W65_001636"
                     /gene_synonym="dgcC"
     CDS             complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="K0W65_001636"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBI3094937.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            45843..>46125
                     /locus_tag="K0W65_001637"
     CDS             45843..>46125
                     /locus_tag="K0W65_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3094938.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKEPERENRELRRSNNILRLAS"
BASE COUNT        11986 a        12032 c        11299 g        10808 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     3181 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     3241 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     3301 tggcgtttct gatgacagca ccagaatacg cttgttataa tagtcagtat tgttatgaag
     3361 acaaagtcag cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat
     3421 atttcacgac tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa
     3481 taagtaatac tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg
     3541 catctttcgc tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga
     3601 gaggactatt ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa
     3661 accgagtgtt ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta
     3721 taaatgccaa agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta
     3781 tgcataatct gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac
     3841 acattacctt tacgcttaat aaattaacaa cacaaccagc aataacaaaa atagatagcg
     3901 tgggtaatga gcaactttat cagattggca aaatctacca taagttggaa attaaacctg
     3961 agccagagca acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg
     4021 caactcaata aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat
     4081 atccagaaag ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc
     4141 gcaccatcgc catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat
     4201 cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt
     4261 tctgagtggc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc
     4321 tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct
     4381 tggcacgcag aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga
     4441 tggttttgct ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg
     4501 actccacttc gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag
     4561 agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata
     4621 cctgcaaagt taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt
     4681 cgcaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga
     4741 ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca
     4801 gcactttgta aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt
     4861 agtgtgaatc tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat
     4921 cccgattttc tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc
     4981 gatgccgtca aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac
     5041 aacggcatgg gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc
     5101 accacccatg ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg
     5161 catatcggtc cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa
     5221 accgtgttgc ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg
     5281 gccgtcaact gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta
     5341 cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc
     5401 gaagggcatc atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc
     5461 acagcagaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc
     5521 gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa
     5581 aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact
     5641 ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt
     5701 tcgcggcggt atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac
     5761 ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga
     5821 atatgacgtg atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac
     5881 tctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg
     5941 cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt ccgattggca atatgaccgg
     6001 cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg
     6061 ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct
     6121 gaaaattatc gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt
     6181 gatggcggca gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct
     6241 gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc
     6301 ttgctcgcgc agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag
     6361 ccaggttttg ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga
     6421 aaccacatca atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat
     6481 atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg
     6541 ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc
     6601 gagaatactg aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac
     6661 cgtcgggctt ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt
     6721 gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag
     6781 gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac
     6841 ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga
     6901 tgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc
     6961 gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct
     7021 gccaaagcgg ttccacagat aatgcgccgc ctgacgcacg ccaatttcat gcgctgcgcc
     7081 gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga
     7141 aacaccggag tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg
     7201 gaaaccacca ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc
     7261 cagatgaaca gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt
     7321 taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg
     7381 cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt
     7441 ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat
     7501 acccggaata cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg
     7561 aacttcgatg ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac
     7621 gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat
     7681 ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc
     7741 cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc
     7801 ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg
     7861 ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat
     7921 tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca
     7981 gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga
     8041 acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt
     8101 acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga
     8161 gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc
     8221 cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc
     8281 gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt
     8341 ggcgttagca caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac
     8401 tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg
     8461 acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg
     8521 ctggcgttct cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt
     8581 tcgatgattt ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca
     8641 ttagatttag acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt
     8701 cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat
     8761 aaagggcgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc
     8821 caggttcagg atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag
     8881 ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc
     8941 cggaagaaag cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa
     9001 cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa
     9061 taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg
     9121 gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc
     9181 tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc
     9241 tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt
     9301 gaccaattga tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc
     9361 cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc
     9421 gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt
     9481 ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg tgtgattggc
     9541 gacggcgcga ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgt
     9601 cctgatatgc tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg
     9661 cttaacaacc atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc
     9721 gggaaaaaag ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa
     9781 catattaaag gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc
     9841 ggcccggtgg acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac
     9901 ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca
     9961 gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg
    10021 ccgaaaagta gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa
    10081 acggcagcga aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc
    10141 atggtcgagt tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa
    10201 cacgcagtga cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc
    10261 tactccacct tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag
    10321 ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac
    10381 cagggcgctt ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg
    10441 agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg
    10501 tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa
    10561 ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt
    10621 ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat
    10681 atgcgttttg tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa
    10741 gcgctggtca ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa
    10801 gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt
    10861 attccgcaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg
    10921 gaagccaaaa tcaaggcctg gctggcataa tccctactcc actcctgcta tgcttaagaa
    10981 attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc
    11041 gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca
    11101 tggacactgc cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata
    11161 aatttctttg ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc
    11221 gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc
    11281 gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc
    11341 ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac
    11401 acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt
    11461 tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa
    11521 cagcactgct gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa
    11581 gaagagcgcg agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc
    11641 ccgctggcgc gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct
    11701 gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag
    11761 cggttaacgg gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg
    11821 ttgttaagta aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt
    11881 gatgagctgc tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa
    11941 acgccgtata aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg
    12001 tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga
    12061 tgaccgataa agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg
    12121 atccccatgc cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata
    12181 tccagaatac ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc
    12241 gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc
    12301 acgcccatat ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc
    12361 accaccagcg agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc
    12421 gccagcgatc ccatcgagcc aggaacgatt gggcttaatc cacttccgaa tccgacagca
    12481 agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac
    12541 aaaatggtca tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag
    12601 cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag
    12661 agcaacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc
    12721 accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc
    12781 agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg
    12841 cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag
    12901 tgcctgcccc tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc
    12961 atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc
    13021 gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc
    13081 tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt
    13141 atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa
    13201 ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg
    13261 atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc
    13321 agcaggatcg atatcaggga ggaaatggtt gcccgccacc agcgtatcag tgctgatcgc
    13381 cagggtctgt ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac
    13441 atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca
    13501 tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt
    13561 ccgtgaatct accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta
    13621 tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg
    13681 atcgcttcgt taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc
    13741 gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag
    13801 ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg
    13861 aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca
    13921 gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg
    13981 cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt
    14041 caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt
    14101 ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac
    14161 cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata
    14221 ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt
    14281 accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac
    14341 aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc
    14401 cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc
    14461 cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc
    14521 ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag
    14581 atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt
    14641 taaattgggg ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt
    14701 cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta
    14761 gcaatacgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac
    14821 ccagttgcat catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg
    14881 tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc
    14941 cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac
    15001 gtactggctg gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag
    15061 accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg
    15121 catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact
    15181 ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa
    15241 tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac
    15301 tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc
    15361 cagcgacctg cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga
    15421 tgagtgcgtc acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat
    15481 acgccgttgc gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg
    15541 gttcacccgc tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc
    15601 acccgacatt cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc
    15661 gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa
    15721 attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat
    15781 cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct
    15841 caccggtggc gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac
    15901 tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg
    15961 gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg
    16021 tggtgaaacg ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc
    16081 ccacgagacg agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac
    16141 ggcgtcctga ccaggtggtt aacggaaatc tattttaccc tgaactgacg atacaacaaa
    16201 ggcgcaacgc ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag
    16261 gctaattgcg acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct
    16321 gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca
    16381 cagcgtcagc acgctgaacc aggagatgac gcaactcaat caagaaacag tcaaaatcac
    16441 tcagcaaaac aggctgaatg caaaatccag cagtggggtt taccttctgc ctggagcgaa
    16501 aacaccggca agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac
    16561 gcctgataca gatggcacca cccttacact acgtattcag ggtgagtcga atgatccctt
    16621 gcccgcattc agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaactttca
    16681 ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga
    16741 tgttgatatt ccgttacaat taaaggggat ttctgtggat cagttaggct ttgtacgtat
    16801 ccacgacatt caacctgtta tgcagtaaac gtatttcggg acgattttgt gcgtcccgca
    16861 acatcttccc cgtcattttg ttactctgct tacatcacct ggattgatag taaaagtttg
    16921 caacaagggc gaaagtcagt acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg
    16981 attacgtaaa tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa
    17041 taggctcctc tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa
    17101 gggattttca aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact
    17161 ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc
    17221 cgactggtgg caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca
    17281 gatccgcaac gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt
    17341 ctatggttat gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg
    17401 gaaccacggt tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac
    17461 caatactgac cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta
    17521 cgacatgggt cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga
    17581 tatcgacact ggcctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca
    17641 gaactatggc gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt
    17701 tgtgccgatt accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga
    17761 ctggggttcc gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac
    17821 taataactct atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc
    17881 tgtcgtagct cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt
    17941 cggcaacggc aacttcaacg ttcgctctac cggctggggt ggttacctgg tagtaggtta
    18001 caacttctga ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt
    18061 gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa
    18121 ttcaatatat tgctgaaata acgtaggcct gataagcgta gcgcatcagg caattttacg
    18181 ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc
    18241 ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc
    18301 ttcggtatat ttcgaatgct catgatcgtg gcaatagaga cacaacaatt cccagttact
    18361 gccatcttcc gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag
    18421 gttagaataa acaaactcgc gggaacagcg accgcagacc cacggataga tttttaatgc
    18481 tttttcgcga tagccgcttt ctaaccgtgc atagtttttt gggatgatag ccatgaaaag
    18541 ttacctggat gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc
    18601 tccatacatc aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt
    18661 gattaaggca gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg
    18721 tgttcgcgct tcatacccag tttcagagcc aacgcagatg ctacgtagat ggaagatgca
    18781 gtgccgatgg aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg
    18841 aagagataca gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc
    18901 agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg
    18961 aagttttcac gaattcggtc cgataccacg atactgtcgt taagcgagta accgataacc
    19021 gacatcaacg atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc
    19081 agcgtaatga taacgtcgtg cgtcagcgca ataaccaccc ctgccgccag tcgccactca
    19141 aagcggaaac ctacgtacac gaggatagac agcagcgctg ccatcaacgc catcgcaccg
    19201 gtttgcgcaa ggtcagcccc cacgctcgga ccgacgaact caatacgctt cactgctgca
    19261 ttctgattgg tggattcgtt aatcaccttc agaacttggc tgcccagcac ctgaccgccg
    19321 gtttcgcctt cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc
    19381 agcattggct cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc
    19441 ggtttttcga gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag
    19501 ccgcgcacgc ccataataac gatagcagcg attaacagca gaccagagat gccgaaagcc
    19561 cagtagtccc agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta
    19621 tattcctgtg ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata
    19681 tagcaggttt acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc
    19741 gataccggta gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat
    19801 gactttaatc agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc
    19861 ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt
    19921 gatcagtacg ttcgcatcga ccgctaccgc aagggttaag acgatacccg cgataccagg
    19981 catactcagt gttgcgcctg gcaacagcga cataataccg acgatcaaga tcaagttggc
    20041 aatcagagca ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat
    20101 agaaaccagc agaccggcaa ggcaagcctc cagcccctgc tcaatgttct gcatacccag
    20161 ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg
    20221 cagcagcagt gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct
    20281 gttacccaga cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac
    20341 cgcacgacca tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat
    20401 cggtttgccg atgttgtcct tggtgaagtt agacataatg ttgccaccag cgctatcgag
    20461 cgagatgtta acctgcggct ggttgtattc gtcctggctg gaagtggagt cggtgatatg
    20521 gtcaccagtc agaattacgc gtttgtacag cacaaccggc tgaccttcgc gggtctgttt
    20581 cacttcagag tcgcccggta cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt
    20641 taccagacgg aattccagcg ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc
    20701 ctgaataccg ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc
    20761 cgccacgcca agttggttta cacggttacg caggatatta atgttctgct gcaccgcata
    20821 ttcacgcgct tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg
    20881 gctgctaatc accaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc
    20941 tttagcatcg cggaaagtga tgctcaggcc gtagttgttt tctttacgaa cagtggtata
    21001 cgggatgccc ttttcgcgca ggtcactgcg taggctatcg atattttgtt cctggagttt
    21061 gccaagcgcg gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag
    21121 gccgagcttc atcggctcag catgaatagc tgccagccag cgcggcgttg ccggggcaag
    21181 gttaagcgcc acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa
    21241 ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt
    21301 agcagttatt ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc
    21361 ggcgactccg cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag
    21421 cgcatacagc agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata
    21481 acggtttaac acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc
    21541 tttcggcagg acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag
    21601 cgcgatagca atgtagccgt tttccgctac tttggttacg cgaccaacca ggccaccgtt
    21661 cgtcagaact tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg
    21721 cttctgctgt ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa
    21781 aatcaaagac atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat
    21841 aaaaaagctc attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt
    21901 ctcgcccctg acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatag
    21961 ccttgcgtaa acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc
    22021 gcgcgcctaa tatttcgttg caacggtcga gatgatgcaa gtaagcgcgt gaataattgc
    22081 gacaggtgta gcaatcacac tcaggatcga gtgggccagt atcgctctta tatttcgcat
    22141 tgcggatttt caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg
    22201 gcattacgca gtcaaacata tcgataccgc gacgtacgcc ttcaaccagg tcttccggtt
    22261 taccaacgcc catcaggtaa cgcggtttgt ctgccggaat ttgcggacac acatgctcca
    22321 gaatacggtg catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat
    22381 caaagccgat atctaccaga cctttaacag atatatcacg taaatcttcg taaatactgc
    22441 cctgaattat accaaataac gcatttttgt taccaagact gtcaaaacgc tcacggctac
    22501 gcttcgccca acgcagagac atctccatag agcgttttgc gtaatcccag tcagcaggat
    22561 acggcgtaca ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca
    22621 tcgacttttc cgggtcgagg aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc
    22681 cctgttcggt gattttacga atatcaccaa ggctgaagac ctggaagccg ccggagtcgg
    22741 taagaatcgg tcctttccac tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt
    22801 cctggcccgg gcgcagccac aggtggaagg tgttgccgag gataatttgc gcgccagtgg
    22861 cttcaacttc ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac
    22921 aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg
    22981 tggtgtccag ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgttta
    23041 aaccagcgcc gcggaattac tccccgacgc gctcattaat tgcctgcgga ttgtacgtga
    23101 taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc ttctaccgcc gctttatagg
    23161 cattcatggt gtgttgataa ccggcaaagg ccgaaaccag cataatcagc gtcgactctg
    23221 gcaagtggaa gttcgtcacc agcgcatcga ccactttgta ctggaagccc ggatagataa
    23281 agatttgggt atcgtcaaag aacggttcga taagatcgtt ttttgccgcc tgtgccgcgc
    23341 tttccagcga acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg
    23401 ccgccagtac cgcgtctacc acatcctgcg gtacttcagc gtattccgag tgcatgatgt
    23461 gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca
    23521 caaacgccat ctccacgcct ttggcgcgta atttttccag caaaggctcg tcaaaatgca
    23581 gaccagcggt cggggctgca accgcgcccg gtttttcgct ataaacggtt tgataaagtt
    23641 cgcggtcagc gtcttcgtcc ggacgatcga tatacggcgg cagcggcata tggccgatgc
    23701 tgttgaggat atccagtacc gagcgttcat cattaaattc gacttcaaac agtgcgccgt
    23761 ggcgcgcggt cattgttgcg ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag
    23821 gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct
    23881 caaccagcac ttcaatcttg ccgccgctgg ctttacgccc aaacagacgc gccgggatca
    23941 cgcgggtatt attaaaaacc agaagatcgc cggggttgag cttatcgagt aaatcggtga
    24001 aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga cagctactgc
    24061 gttcaggcat gggatagtgg gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa
    24121 cgcgcatgac actgactcag actaaaataa gagtcgggta gtctagtgcc ggggcgctct
    24181 ccctgcaacc tttacccccc tttggataga atataatcga tgaatttttt agctcacctg
    24241 catttagccc atctcgcgga aagctcgctt tctggcaatt tactggctga tttcgtacgc
    24301 ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg gcattcatat gcatcgacgt
    24361 attgactgac aatctgccgg aagtccgcga agcacaggag tggtttcgta gtgaaacgcg
    24421 ccgcgttgcg cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc
    24481 gcagctgtcg ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat
    24541 gacgattttg ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga
    24601 gcagtggctg gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc
    24661 aagccgccgc ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta
    24721 tgacgccctc gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca
    24781 caaggcgtta taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga
    24841 atcccgtcag tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagaaac
    24901 ggtgacgcgg gtagcaccac ttcactggcc tcgccacggt tgattgccac cagtacacgt
    24961 tgctgattca gcacgcggac aaataccact acgttatctt ccgcatacag cacctgacag
    25021 ccgccacgac gcagcgcctg acttttctta cgcagcacaa tcattcgccg gtacagcgcg
    25081 aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg
    25141 tttttaccat ccagtcccac ttcatcaccg taataaatgc acggtacacc aggccaggtg
    25201 aacagccaga ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat
    25261 cgcgcagtat cgtggctatc gagctgatta aacatacgta attgttgttg atgagaaagc
    25321 cctgcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg ctgcggatca
    25381 taagagatgt cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc
    25441 atggcagcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg
    25501 acaatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccggc aacgtgctgc
    25561 atattattgc gcgccccacc cgcctccccc agcatatgca ccacatccag ccgccagccg
    25621 tccatattcc acggcgcttt cagccagtgg cggacaatac tgtcttcccc gcgataaatt
    25681 tcattcacca gactttccga ctgataatcc agcttcgtca agctggcata gccaagccag
    25741 tcgagcgccg tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagagttg
    25801 tgacaagctc cccccgtgcc aagattgtgc ctgtcaaacc aggcatggga atcgccactg
    25861 tggttaaaca cgccgtccag caccagccgc attcccagct gctgcgtatt gtgtcgcaaa
    25921 cgcagcaacg ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg
    25981 tatttatgta cgctgggagc tttaaacacc ggattgagat acagcgctgt cacgccaagc
    26041 tttttcagat acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat
    26101 ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct
    26161 gcatgatggt aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga
    26221 tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat atcaggtaca
    26281 tcgacggcaa actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac
    26341 caacgctggc gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg
    26401 gagagatcaa tcgccgcacg ccatgcggtg acgcctggct gcggctgact gcgctgctta
    26461 tgcattggta ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt
    26521 gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat
    26581 gggggcaccg gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat
    26641 caccttgcca tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg
    26701 acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag
    26761 aacgcaagta ttattggttt tcagccagct gacgatcgtg gcggcgttta aacatccagc
    26821 caatcaacag cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt
    26881 gataaccaat caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta
    26941 ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga
    27001 aggcgatttg cgacagcagg atcataatcc acacccatac cgtggcgaag gttgccagcg
    27061 aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca
    27121 gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga
    27181 aaattttcgg tgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata
    27241 cgtcactgtt aattgccgac agcgaagcgg tcagcacaac aaagttaaga atgctggcgg
    27301 caaaggtaat gcccatatgc tggaacgtca gtacgaacgg gctaccggca gtgccaacct
    27361 gattccacgg gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac
    27421 gcatcggcac ggagttaatc gcacgcggaa tcgatttctc aggatctttc gcttcaccgg
    27481 cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatta
    27541 ccatgccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg
    27601 tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga
    27661 tgatgatggt ggcgacttta aagaacgaga accagaattc cagctcaccg aagaccttca
    27721 cgctcatcag gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg
    27781 gcaccgtcgg gaacaagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga
    27841 caataaggat ttcaaaacag taggtccatc cggtaatgta acctgccagc gggccaaggt
    27901 tttcctgcgc ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca
    27961 gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg
    28021 gaccggccat tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac
    28081 ccagtgccat aaagcgtatg tggcgggtac ttagcccacg ctttagctta ttcttacttt
    28141 ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt
    28201 aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat
    28261 cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt
    28321 cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga
    28381 tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg
    28441 aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa
    28501 tagcggtcag caccggtacg gagatctgga tcagctggct taagccgagg ttagacacca
    28561 ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg
    28621 caaagaattc tgcacaagca caggtcaggc caaccgccgt gaccagacag gcgatgaaga
    28681 ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca
    28741 gaatagcagc gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacaga
    28801 acagcgccag gtacagcaaa gtcagaccaa cacccgccat caggccagcc cagacggtat
    28861 aacgggtcag cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga
    28921 taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag
    28981 aaaatgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa
    29041 tcgcgacaac agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca
    29101 cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca
    29161 ggctgtagat aaacagcggc agcgcggaat cacccgtcag cggcgcaatc cccacttcaa
    29221 aggaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg
    29281 ttgccagcag tacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc
    29341 caacttttgc cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc
    29401 cgaatgccgc agtccagacg tgttcgcctg cctgtaaacc gaccattgga gggaaaataa
    29461 tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg
    29521 atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt
    29581 tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac
    29641 tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg
    29701 acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa
    29761 accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa
    29821 agacatggct aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat
    29881 tttgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa gatgacaaag
    29941 gcggattaat cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt
    30001 ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc
    30061 acgatcgcta accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga
    30121 taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca
    30181 acgctaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttcc
    30241 ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg
    30301 ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc
    30361 agagtctgag cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg
    30421 agcaattgcg tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct
    30481 tccatccgct gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc
    30541 agcggctgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac
    30601 tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg
    30661 acatcacgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga
    30721 tgccgcccgg tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat
    30781 tgcgtaaact ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag
    30841 cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga aaataccgcc ctcttccgtg
    30901 gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg
    30961 cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc
    31021 ggttcccagc taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat
    31081 gaaaggcgca ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa
    31141 aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc
    31201 agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag
    31261 aaatcataag ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga
    31321 tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta
    31381 cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag
    31441 acgtggttta acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc
    31501 agcagtttaa attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga
    31561 gatgtcgggt tgagacttaa tccctgcatc ttaatcacct cttccaccgc cattggcgaa
    31621 atacggcgca ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa tggcttggtg
    31681 atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg
    31741 gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttgatgaac
    31801 tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca
    31861 ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt
    31921 tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt
    31981 ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat
    32041 gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt
    32101 tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg tataatcgcg accatgcttt
    32161 ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact
    32221 tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag
    32281 cacaaaccca tcaggtggat gcgattattg ttgctggtga tgttttcgat accggctcgc
    32341 cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct
    32401 gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg
    32461 atatcatggc gttcctcaat acaaccgtgg tcgccagcgc cggacatgcg ccgcaaatct
    32521 tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgcccgc
    32581 gtgacattat ttccagccag gcggggctta acggtattga aaaacagcag catttactgg
    32641 cagcgattac cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc
    32701 agcctctgcc catcatcgcc acgggacatt taacgaccgt gggttccagt aaaagtgacg
    32761 ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag
    32821 ccgactacat cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg
    32881 ttcgctattg cggttccccc attccactga gttttgatga atgcggtaag agtaaatatg
    32941 tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg
    33001 taactcaacc catggcggtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac
    33061 agtggcgcga tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg
    33121 agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag
    33181 tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg
    33241 aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagacc
    33301 tggatgaatc gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cgtaccctcg
    33361 ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa
    33421 aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgccat
    33481 taccggccca accggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta
    33541 tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga
    33601 taccgctgaa tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt
    33661 ctggagccag aatcgggcgc gtaaccagcc ggacggtaat ttgcaggtgc ctcgcgtaga
    33721 gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact
    33781 gacagcgacg ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc tgctttcgca
    33841 ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga
    33901 gttaaccggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc
    33961 ggcccgcact gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc
    34021 ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt
    34081 aattaccgcg cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt
    34141 gcagcaagaa gccagccgcc gtcaacaggc cttgcaacag gcgttagccg aagaagaaaa
    34201 agcgcaacct caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca
    34261 ctgggaacgc atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga
    34321 agtaaatact cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc
    34381 gaagcagtca gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca
    34441 cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca
    34501 aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa
    34561 acttaatgcg cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctaccgccct
    34621 ggcgcaacat gccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat
    34681 tgttccccaa caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga
    34741 acagacgcaa cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca
    34801 gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc
    34861 ccagcgtgca cagttacagg cgggtcagcc ttgtccactt tgtggttcca ccagccaccc
    34921 ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct
    34981 ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc
    35041 attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca
    35101 agcacttact caacaatggc aagccgtcac ggccagcctc aatatcacct tgcagccaca
    35161 ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact
    35221 cagccaacga catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta
    35281 tcaacagcaa attgaacaac gtcagcagca gctcttaacg gcattggcgg gttatgcact
    35341 gacattgcca caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca
    35401 gagctggcag caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac
    35461 accgattctg gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc
    35521 gctggataac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac
    35581 gttacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccaag cgcagtttga
    35641 caccgcgcta caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga
    35701 tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca
    35761 ggcgcaaacg ctggtcactc agacagcaga aacgctggca cagcatcaac aacaccgacc
    35821 tgacgggttg gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca
    35881 ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga
    35941 tgcagataac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa tgacgcagca
    36001 ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg
    36061 caagtttgcc caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac
    36121 ccggctgcac gtgcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt
    36181 tgttgatacc tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag
    36241 tttcctcgtt agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg
    36301 tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac
    36361 cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca
    36421 cgtagaagcg atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct
    36481 gggctacagc aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa
    36541 tacagagggg tgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta
    36601 ttggctacgg tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt
    36661 ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat tatgagcgtg
    36721 ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg
    36781 tattatgcac tggtggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac
    36841 tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc
    36901 acgctctctt cgtcttacct gatgctcgcc attggtcggc tggtatccgg ctttccgcat
    36961 ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc
    37021 accgccgccg tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg
    37081 ctgggaacgt atttaagtca ggaatgctgg cgttacacct ttttattgat cgctgttttt
    37141 aatattgcgg tgatggcatc ggtctatttt tgggtgccag atattcgcga cgaggcgaaa
    37201 ggaaatctgc gcgaacaatt tcactttttg cgcagcccgg ccccgtggtt aattttcgcc
    37261 gccacgatgt ttggcaacgc aggtgtgttt gcctggttca gctacgtaaa gccatacatg
    37321 atgtttattt ccggtttttc ggaaacggcg atgaccttta ttatgatgtt agttgggcta
    37381 gggatggtgc tgggaaatat gctaagtggc aggatttcag gacgttattc accactgcgc
    37441 attgcagcag tgactgactt tatcattgta ctggcactgc tgatgctctt tttctgcggc
    37501 ggcatgaaaa caacgtcgct tatttttgct tttatttgtt gcgcgggatt atttgccctt
    37561 tcagcacctc tgcaaatatt gttactgcaa aatgccaaag gaggagagtt attaggtgcc
    37621 gcaggtgggc aaatagcgtt taacctcggt agcgccgtcg gcgcatattg cggaggtatg
    37681 atgctgacgc tggggctggc atataattac gtggcgctgc ctgccgccct gctttcgttt
    37741 gctgcgatgt catcgttgct gctgtatggt cgctataagc gccagcaagc ggcggatagt
    37801 ccggtgctgg cgaaaccact ggggtagatt actgtcacgg tgcgcgttgc ttattgccgg
    37861 atgcggcgtg aacgccttat ccgccctacg cggttctggc acattttgta ggcctgataa
    37921 gacgcggcaa gcgtcgcatc aggcatgatg cgccaattgc ctacgttttt tactcttgtg
    37981 gccataacca cgcagcgccg cgtacgccgc tggaatcacc gtgcttcgcc ttactcaccg
    38041 gcgtttcaca ttcgccgccg aagacaaatt gtttaatcaa ctgcccaacc gtttgatata
    38101 aacggtctac attgctcatc ccgcccccca ggacaatcac atccggatcg agaatattca
    38161 cgacatgtgc cagcgatttt gccagccgca gctcgtagcg acgcaatgcc agttccgcta
    38221 ccggatcgct ttcttcaacc aggcggataa tttcactgcc tttcagcgca tgtccgctca
    38281 aacgacgata atccgtcgcg aatcccgtgc ccgaaataaa ggtttcaata caaccttgtt
    38341 taccgcaata acaagggact tcctcgcgat aacgcagttc gtcttcgtcc atccacggta
    38401 gcggattgtg tccccactca cctgccgtgc cattgccgcc gatatgcgtc cgcccattga
    38461 atgccacgcc cgcgccgcat cccgtgccga taatcacggc aaataccgtc tgcgctcccg
    38521 ctgccgcgcc atctactgct tctgaaaccg ccagacagtt ggcatcattt gccagccgca
    38581 cttcccgctg caacctcgcg cttaagtctt tatcgaatgg ctgaccgttg agccaggttg
    38641 aattgacatt cttcaccaca ccggtgtagg gcgaaatcga acctggaatg cccataccta
    38701 ccgttccgcg ctgccctgtc gcctgctccg ccatatcaac caacgtggcg atcgtttcaa
    38761 tagtctgccg gtaatcatca cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg
    38821 catcgcccag tgcaatcact tcagttttgg tgccgcctaa atcgatacct atacgcactg
    38881 tactctcctt atttttttca atatcaatag cgtagagacg gacaaccgga ttggcaatgc
    38941 aaggccgccg acaattcgtt atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta
    39001 tcatgctgtg gttcaaaaat ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg
    39061 cagaagagat ggaaaaacag ctagcctcga tggcatttac cccatgcggc agccaggaca
    39121 tggcgaagat gggctgggtt cctccgatgg gatcgcacag cgatgcgtta acgcacgttg
    39181 ccaatggtca aattgttatc tgcgcgcgca aagaagaaaa aatcctcccg tctccggtga
    39241 ttaaacaggc gctggaagcg aaaatcgcca aactggaagc ggaacaggcg cgtaagctga
    39301 agaaaaccga aaaagattcg ctgaaagacg aagtgctgca ctctctgctg ccgcgtgctt
    39361 tcagccgttt tagccagaca atgatgtggc tcgacacggt taacggtttg attatggtgg
    39421 actgcgccag tgccaaaaaa gcggaagata cgctggcatt actgcgtaaa agcctggggt
    39481 cgttaccggt tgtaccattg agcatggaaa acccgattga actgacgctg accgaatggg
    39541 ttcgctccgg tagtgcggca cagggcttcc agctgcttga tgaagccgag ctgaaatcgt
    39601 tgctggaaga tggcggcgtg atccgcgcga agaaacaaga tctgaccagc gaagagatca
    39661 ccaatcacat tgaagccgga aaagtggtga ctaaactggc gctcgactgg cagcagcgca
    39721 ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct taagttctgc gacgagctgc
    39781 gcgatcaaaa cgaagatatc gaccgtgaag atttcgccca gcgttttgat gccgatttca
    39841 tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg
    39901 aagcacaacg ttaattgctg attttccttt aatgccggat gcgacgcctg ccgcgtctta
    39961 tccggcgtac gaagccacac caggcatata attattcgct acggcgagca ataattttta
    40021 gcgcagcaat attatgcgtt ttacgccgta actgatctaa tcgcctcatc cctattcgcc
    40081 tgtcagcagc acgcagaata ttcactaacc cccatttatc gctggtaaat tgttttgtta
    40141 acagctccgc ttttggggta tcaatatatt cacgcaataa atcagaaatt gaacaaatat
    40201 ccgtcaggta aggataccag caagtttcac cgtgaaaatt acgcactcca gcgttacctg
    40261 caataaagtc tttcgggtaa tcccagatca cgtttttatc caacgtgatc caataacgcg
    40321 gtaaatcggt acttccacca ttttggctgc gcatgggata acgggtacag tgaatttgca
    40381 ggttgatcgt tggcgtcagt aagtcataga tctcccgctg taacttgctc catggacgtt
    40441 gtgtcattgt ttttcctcaa gccggtgacg cgcttcactg cgaattttcc gcgttgcccc
    40501 ggttttcact aaatcacgta actgcgaaag atcgggttgt tcgacaaata agagtaatga
    40561 tggatcctct tttaaccacg ccgttttcac atcggcagcc agctgtggat tattaatagc
    40621 cagcgaccga tcctgaggct cagtcaatgt cgttaacaac gacgccgggg tatgtgcgtt
    40681 gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc agcaatgcac gccaccattc
    40741 atcatctgat ttatctttta tcgtagccag ttccgcaaaa aatacctgga gatcttcact
    40801 gatatttata tcaaccttga tgaattatga atatatcgga aattgaaaaa atcgcagcaa
    40861 aggaaatatt attatgggtt aaagagagga tatttcagaa aaatgccctc ccgttcaagg
    40921 ggaaggcgag gaattacaga tagcggcaca gataagaggt aggttcggca acttgaagat
    40981 gaaactcact gtgaccggga acattaaaca ccgaaccggc ttcatacact tgccagtcgg
    41041 tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag
    41101 cggtgctgaa ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc
    41161 tgctggaaaa gccgatcgat ttcactttgc cggaaaagta ctcattactt tgaagcataa
    41221 actggccctg tctggtagtt gaacatctac ttcactatag gggccagagg cgctggctgt
    41281 cacgcaaaat tacaccagta attccgcagc cagccgtgca atcaatacgt tagagagcaa
    41341 gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca
    41401 atccagcatg atgacatcag ccccttttgc cagtaattct ttcccagcat caatgatttt
    41461 ttgttctgaa tcatgaatgg ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg
    41521 ccatttttgc gcctgaacgg gcagcatctc ctcaaccgga acgataaccc ccacctgatg
    41581 atcttcaaca atagaggaaa ccagtggagg caatattcgc gacggctcaa gaaagatcgt
    41641 attacgcgca gtcatactac taatgtttgc tgtactcatt aataaaatga cgtcataacc
    41701 ctgattatcg agcacttcaa ccacaccttg cagatctcgc tccactttac gacgcgaaac
    41761 atgggccagc tggttgtcat ttaataatgt gagaatggtg tcttcgcctg cttctggcgc
    41821 gtactccgcc atcacttctt cacgacttaa cttccccagc aggctatgat gggaaatatt
    41881 atcttcgtca atgtattccg tcaggagcgg caaaacctcc tgcataggta caatgccgat
    41941 ggtcaggatc gccaacgacg cactcattgt tcatccacct tacttttctt tcgtcgttaa
    42001 ttaccgggca agtgtgaagc accactgctg acacttactt gtgctacaaa tgacaaaaag
    42061 cgtagcagca gacgtgcacg gcataatgta aagattccaa atgattccag taatggaatg
    42121 ttattgttta atattctaat tattagaaaa acataaatta tgaaaaatgt gacgcagatc
    42181 ccttgtagtg gcactcagaa tcccttccga gcagtctgat gcggtcactc tttgtcaggg
    42241 tcctcatagc gttttttcgc atccatcgct tcctgagccg tcggatgttc actgatcaac
    42301 gagtctggtt taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt
    42361 cctttttcaa tcgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt
    42421 cttccttatt tcacgggatg aacgttaagt ataggcgctc taaaatcaac aattgatcgt
    42481 ctgtgccagg gcgctacgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat
    42541 gataatatgc gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc
    42601 ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag
    42661 tcggttaacc aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat
    42721 gaagtgacga ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgc
    42781 cgcagttacc gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct
    42841 ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac
    42901 aaaccgacgg ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg
    42961 aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg
    43021 gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt
    43081 aaagaaaaaa ttccacttaa agtaaaaatc tcaatacatc ccttactata ccaataaata
    43141 ttcaagaatg aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc
    43201 caccacttct ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt
    43261 ccgccggtct ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcac
    43321 tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcgaat taagcgccgc tgcgcctttc
    43381 tcgatggctt cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac
    43441 tgccggacaa tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata
    43501 aatcgcgatg gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata
    43561 cgttccgccg cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc
    43621 caaattgtca taaagtcatc ctttgttagg taatcctcta ttgtgtcgcg cttttgcctt
    43681 ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag
    43741 gagtgaggca atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat
    43801 tctcggcggt ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatacacccc
    43861 ctccccggat aaagtcgccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc
    43921 gcaagaagtg gcgcaaatcg ccgacatcat ttttgctgcc gttaaacctg gcatcatgat
    43981 taaagtgctt agcgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc
    44041 tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg
    44101 cgccatgccg aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc
    44161 gctggtaacc ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc
    44221 ggaagtaatt gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc
    44281 ctacgtattt atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg
    44341 cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga
    44401 aacgggagaa catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat
    44461 tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac
    44521 gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag
    44581 gccgccactt cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc
    44641 gatttcaacc actcacgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta
    44701 atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc
    44761 cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg acatgattac tgcaaactca
    44821 tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta
    44881 agcgccacaa tcgcttcatc gcccacatca tgaccccagg tatcgttgat gctcttgaaa
    44941 tggtcgatat cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat
    45001 tcattgcgta acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcga
    45061 gtactcatga cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg
    45121 acccagccaa acatcagagg ataaacgaca ataatgggaa gggagagcca ccattccagc
    45181 ggagcactat tgaacgacac ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc
    45241 accatcaaca ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga
    45301 cacagaatca tcaacatagc ggtggaaggc agcacgttta cgcccattac gcctacccac
    45361 attcccgcta atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg
    45421 gccctgctcg ctatctgcca ggctaaatgc ggccagacga acgcccagcc gaccaacacc
    45481 agccaccacc agcccggcgg cgggtgcgaa accagcgttg aagcaatcgg taagaacatg
    45541 ccagccaggc caaccgcacg gggtagtctg acgcgacggg cgaagcgcag cccggaccgc
    45601 tgatgttcgt tttgaggagt taaaggaggt tcctccccat gcgccgccgc ttttttgaaa
    45661 aagttttcat cattcattat ttttgggaac attcagaaac aattttccca aattatagag
    45721 acggggctaa aggcagaagt atgaaatttc gggtgagcca ctttcgtagc tcacccgtta
    45781 gcactatctg aaccgccccg ggaatcctgg agactaaact tcctgagaaa gaggtaaaca
    45841 ggatgactaa aaatactcgt ttttcccccg aagtccgtca acgggcagtc cgtatggttc
    45901 tggaaagtca gggcgaatat gactcacaat gggcgacaat ttgttccatt gccccaaaga
    45961 ttggctgtac gccggagact ctgcgtgtct gggttcgcca gcatgagcgg gataccgggg
    46021 gcggtgatgg agggctcacc accgctgaac gtcagcgtct gaaagagccg gagcgtgaaa
    46081 atcgtgaact gcgccgcagt aacaatatcc ttcgcctggc ttccg
//
LOCUS       DAETQL010000028        42872 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.028, whole genome shotgun sequence.
ACCESSION   DAETQL010000028 DAETQL010000000
VERSION     DAETQL010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42872
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.028"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(91..627)
                     /gene="ssb1"
                     /locus_tag="K0W65_001638"
     CDS             complement(91..627)
                     /gene="ssb1"
                     /locus_tag="K0W65_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBI3094939.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPYNEPPMDFDDDIPF"
     gene            881..3703
                     /gene="uvrA"
                     /locus_tag="K0W65_001639"
     CDS             881..3703
                     /gene="uvrA"
                     /locus_tag="K0W65_001639"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBI3094940.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3738..4094)
                     /locus_tag="K0W65_001640"
     CDS             complement(3738..4094)
                     /locus_tag="K0W65_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBI3094941.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4098..4514)
                     /locus_tag="K0W65_001641"
     CDS             complement(4098..4514)
                     /locus_tag="K0W65_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBI3094942.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4625..5338)
                     /gene="aphA"
                     /locus_tag="K0W65_001642"
     CDS             complement(4625..5338)
                     /gene="aphA"
                     /locus_tag="K0W65_001642"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBI3094943.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5740..5944
                     /locus_tag="K0W65_001643"
                     /pseudo
     CDS             5740..5944
                     /locus_tag="K0W65_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6447..7640)
                     /gene="tyrB"
                     /locus_tag="K0W65_001644"
     CDS             complement(6447..7640)
                     /gene="tyrB"
                     /locus_tag="K0W65_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBI3094944.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7893..8972)
                     /gene="alr"
                     /locus_tag="K0W65_001645"
     CDS             complement(7893..8972)
                     /gene="alr"
                     /locus_tag="K0W65_001645"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBI3094945.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9025..10440)
                     /gene="dnaB"
                     /locus_tag="K0W65_001646"
     CDS             complement(9025..10440)
                     /gene="dnaB"
                     /locus_tag="K0W65_001646"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBI3094946.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10523..11506
                     /locus_tag="K0W65_001647"
     CDS             10523..11506
                     /locus_tag="K0W65_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBI3094947.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11672..11914)
                     /gene="pspG"
                     /locus_tag="K0W65_001648"
     CDS             complement(11672..11914)
                     /gene="pspG"
                     /locus_tag="K0W65_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBI3094948.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12048..13085)
                     /gene="dusA"
                     /locus_tag="K0W65_001649"
     CDS             complement(12048..13085)
                     /gene="dusA"
                     /locus_tag="K0W65_001649"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBI3094949.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13448..14433)
                     /locus_tag="K0W65_001650"
                     /pseudo
     CDS             complement(13448..14433)
                     /locus_tag="K0W65_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14751..15266
                     /gene="zur"
                     /locus_tag="K0W65_001651"
     CDS             14751..15266
                     /gene="zur"
                     /locus_tag="K0W65_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBI3094950.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15308..15517)
                     /locus_tag="K0W65_001652"
     CDS             complement(15308..15517)
                     /locus_tag="K0W65_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBI3094951.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15633..16958)
                     /gene="dinF"
                     /locus_tag="K0W65_001653"
     CDS             complement(15633..16958)
                     /gene="dinF"
                     /locus_tag="K0W65_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBI3094952.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17031..17639)
                     /gene="lexA"
                     /locus_tag="K0W65_001654"
     CDS             complement(17031..17639)
                     /gene="lexA"
                     /locus_tag="K0W65_001654"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBI3094953.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17749..18117)
                     /gene="dgkA"
                     /locus_tag="K0W65_001655"
     CDS             complement(17749..18117)
                     /gene="dgkA"
                     /locus_tag="K0W65_001655"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBI3094954.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18288..20711
                     /gene="plsB"
                     /locus_tag="K0W65_001656"
     CDS             18288..20711
                     /gene="plsB"
                     /locus_tag="K0W65_001656"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBI3094955.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20866..21738)
                     /gene="ubiA"
                     /locus_tag="K0W65_001657"
     CDS             complement(20866..21738)
                     /gene="ubiA"
                     /locus_tag="K0W65_001657"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBI3094956.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21751..22248)
                     /gene="ubiC"
                     /locus_tag="K0W65_001658"
     CDS             complement(21751..22248)
                     /gene="ubiC"
                     /locus_tag="K0W65_001658"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBI3094957.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22471..24051)
                     /locus_tag="K0W65_001659"
                     /pseudo
     CDS             complement(22471..24051)
                     /locus_tag="K0W65_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24280..25200)
                     /gene="malM"
                     /locus_tag="K0W65_001660"
     CDS             complement(24280..25200)
                     /gene="malM"
                     /locus_tag="K0W65_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HBI3094958.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25352..26692)
                     /gene="lamB"
                     /locus_tag="K0W65_001661"
     CDS             complement(25352..26692)
                     /gene="lamB"
                     /locus_tag="K0W65_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBI3094959.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26764..27879)
                     /gene="malK"
                     /locus_tag="K0W65_001662"
     CDS             complement(26764..27879)
                     /gene="malK"
                     /locus_tag="K0W65_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBI3094960.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28244..29434
                     /gene="malE"
                     /locus_tag="K0W65_001663"
     CDS             28244..29434
                     /gene="malE"
                     /locus_tag="K0W65_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBI3094961.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29588..31132
                     /gene="malF"
                     /locus_tag="K0W65_001664"
     CDS             29588..31132
                     /gene="malF"
                     /locus_tag="K0W65_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HBI3094962.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31147..32037
                     /gene="malG"
                     /locus_tag="K0W65_001665"
     CDS             31147..32037
                     /gene="malG"
                     /locus_tag="K0W65_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBI3094963.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32408..33883
                     /gene="xylE"
                     /locus_tag="K0W65_001666"
     CDS             32408..33883
                     /gene="xylE"
                     /locus_tag="K0W65_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HBI3094964.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33927..34337)
                     /gene="psiE"
                     /locus_tag="K0W65_001667"
     CDS             complement(33927..34337)
                     /gene="psiE"
                     /locus_tag="K0W65_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBI3094965.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34463..34606)
                     /locus_tag="K0W65_001668"
     CDS             complement(34463..34606)
                     /locus_tag="K0W65_001668"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094966.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34607..34885
                     /locus_tag="K0W65_001669"
     CDS             34607..34885
                     /locus_tag="K0W65_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094967.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34932..37028)
                     /locus_tag="K0W65_001670"
     CDS             complement(34932..37028)
                     /locus_tag="K0W65_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBI3094968.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37028..37765)
                     /locus_tag="K0W65_001671"
     CDS             complement(37028..37765)
                     /locus_tag="K0W65_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBI3094969.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37762..38400)
                     /locus_tag="K0W65_001672"
     CDS             complement(37762..38400)
                     /locus_tag="K0W65_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBI3094970.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38514..38756)
                     /locus_tag="K0W65_001673"
     CDS             complement(38514..38756)
                     /locus_tag="K0W65_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3094971.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39219..40868)
                     /gene="pgi"
                     /locus_tag="K0W65_001674"
     CDS             complement(39219..40868)
                     /gene="pgi"
                     /locus_tag="K0W65_001674"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBI3094972.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41393..42742
                     /gene="lysC"
                     /locus_tag="K0W65_001675"
     CDS             41393..42742
                     /gene="lysC"
                     /locus_tag="K0W65_001675"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBI3094973.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
BASE COUNT        10673 a        11027 c        10523 g        10649 t
ORIGIN      
        1 agcctccata acggaggctt cttttttatg gctggcagat gctttaatca tccaccttaa
       61 aacaatataa cctattgttt taatgacaaa tcagaacgga atgtcatcat caaagtccat
      121 cggcggctcg ttatacggcg ctgccggagc ggactgctgc gggcgagact gcgcgccgcc
      181 gctgaactga ttgccaccct gcggctgctg aggctgaccc caaccgccct gcggctgacc
      241 accaccgata ttgccacctg ccggagcgcc accaccctga cgaccaccca gcatctgcat
      301 ggtgccgcca acgttcacca cgacttctgt ggtgtagcga tcctgaccgg attgatcggt
      361 ccatttacgg gtacgcagct gaccttcgat ataaacctga gaacctttac gcagatattc
      421 gctcgccact tctgccagtt tgccgaacag cacaacgcgg tgccattcag tctgttcttt
      481 catctcgccg gtcgctttat cacgccagga ttcggaagta gccagcgtaa tgttggcaac
      541 tgcgccacca tttggcatgt agcgtacttc cgggtcctga cccagattac caacgagaat
      601 aaccttgttt acgcctctgc tggccatgtt cgtgtctcct gaaaaaaatc gttctgaata
      661 agtgtaaacg cgcgattgta ccattaccaa tagcgctttt actatgttgt gacctcggtt
      721 ccgggaaaca aacctggcca gacattgtta cacaacactc cggataatgc attccaatac
      781 tgtatattca ttcaggtcaa tttgtgtcat aattaaccgt ttgtgatcgc cggtagcacc
      841 atgccaccgg gcaaaaaagc gtttaatccg ggaaaggtga atggataaga tcgaagttcg
      901 gggcgcccgc acccataatc tcaaaaacat caacctcgtt atcccccgcg acaagctcat
      961 tgtcgtgacc gggctttcgg gttctggcaa atcctcgctc gctttcgaca ccttatatgc
     1021 cgaagggcag cgccgttacg ttgaatccct ttccgcctac gcgcggcagt ttctgtcact
     1081 gatggaaaag ccggacgtcg atcatattga ggggctttct cctgccatct caattgagca
     1141 gaaatcgacg tctcataacc cgcgttctac ggtggggaca atcaccgaaa tccacgacta
     1201 tttgcgtttg ttgttcgccc gcgtcggcga accgcgctgt ccggaccacg acgtcccgct
     1261 ggcggcgcaa accgtcagcc agatggtgga taacgtgctg tcgcagccgg aaggcaagcg
     1321 tctgatgctg ctcgcgccaa tcattaaaga gcgcaaaggc gaacacacca aaacgctgga
     1381 gaacctggca agccagggtt acatccgtgc tcgtattgat ggcgaagtct gcgatctttc
     1441 cgatccgccg aaactggaac tgcaaaagaa acataccatt gaagtggtgg ttgatcgctt
     1501 caaggtgcgt gacgatctta cccaacgtct tgcggagtcg tttgaaaccg cgctggaact
     1561 ttccggtggt acagcggtag tggcggatat ggacgacccg aaagcggaag agctgctgtt
     1621 ctccgccaac ttcgcctgcc caatttgcgg ctacagtatg cgtgaactgg agccgcgact
     1681 gttttcgttt aacaacccgg caggtgcctg cccgacctgt gacggccttg gcgtacagca
     1741 atatttcgat cctgaccgcg tgatccaaaa ccccgagctg tcactggctg gcggtgcgat
     1801 ccgtggctgg gatcgccgca acttctatta cttccagatg ctgaaatcgc tggcagatca
     1861 ctataagttc gacgtcgaag cgccgtgggg cagcctgagc gcgaacgtgc ataaagtggt
     1921 gttgtacggt tctggcaaag aaaacattga attcaaatac atgaacgatc gtggcgatac
     1981 ctccatccgt cgtcatccgt tcgaaggcgt gctgcacaat atggagcgcc gttataaaga
     2041 gacagaatcc agtgcggtac gtgaagaatt agccaagttt atcagcaatc gcccatgcgc
     2101 cagctgcgaa gggacgcgtc tgcgtcggga agcgcgccac gtttatgtcg agaatacgcc
     2161 gctgcctgct atctccgaca tgagcatcgg tcatgcgatg gaattcttca acaatctcaa
     2221 actcgctggt cagcgggcga agattgcgga aaaaattctt aaagagatcg gcgatcgcct
     2281 gaaattcctc gtaaacgtcg gcctgaatta cctgacactt tcccgctcgg cagaaacact
     2341 ttccggcggt gaagcccagc gtatccgtct ggcgagccag attggtgcgg gcctggttgg
     2401 cgttatgtac gtgctggacg agccgtctat cggcctgcac cagcgcgata acgagcgcct
     2461 gttgggtacg cttatccatc tgcgcgatct cggtaatacc gtgattgtgg tggagcatga
     2521 cgaagacgca attcgcgccg ctgaccatgt gatcgatatc ggtcctggtg cgggtgttca
     2581 cggcggtgaa gtggtcgcag aaggtccgct ggaagcgatt atggcggtgc ctgaatcgtt
     2641 gaccgggcag tacatgagcg gtaaacgcaa gattgaagtg ccgaagaaac gcgttccggc
     2701 gaatccagaa aaagtgctga agctgacagg cgcacgcggc aacaacctga aggacgtgac
     2761 gctcacgctg ccagtcggtc tgtttacctg catcacaggg gtttcaggtt ccggtaaatc
     2821 gacgctgatt aacgacacac tgttcccgat tgcccaacgc cagttgaatg gtgcgaccat
     2881 cgccgaaccg gcaccgtatc gcgatattca gggactggag catttcgata aagtgatcga
     2941 tatcgaccaa agcccaattg gtcgtactcc acgttctaac ccggcgacct ataccggcgt
     3001 gtttacgcct gtgcgcgaac tgtttgcggg cgtaccggaa tcccgtgcgc gtggttatac
     3061 gccaggacgt ttcagcttta acgtccgtgg cgggcgctgc gaagcctgtc agggcgacgg
     3121 tgtgatcaaa gtggagatgc acttcctgcc ggatatctac gtgccgtgcg atcagtgcaa
     3181 aggtaaacgc tataaccgtg aaacgctgga aattaagtac aaaggcaaaa ccatccacga
     3241 agtgctggat atgaccatcg aagaggcgcg tgagttcttt gatgcggtgc cagctctggc
     3301 gcgtaagctg caaacgttga tggacgttgg tctgacgtac attcgcctgg ggcagtccgc
     3361 aaccacactt tctggtggtg aagcccagcg cgtgaagctg gcgcgtgagc tgtcaaaacg
     3421 cggcaccggg cagacgctgt atattctcga cgagccgacc accggtctgc acttcgccga
     3481 tattcagcaa ctcctcgacg tgctgcataa actgcgcgat cagggcaata ccattgtggt
     3541 gattgagcac aatctcgacg tgatcaaaac cgctgactgg attgtcgacc tgggaccaga
     3601 aggcggcagt ggcggcggcg aaatcctcgt ctccggtacg ccagaaaccg tcgcggagtg
     3661 cgaagcttcg catacggcac gcttcctcaa gccgatgctg taatcgttaa ggccgctttc
     3721 tgagcggcct tttcctttca gagttgtacc agcaatttac gcttttcttc cggtagtaaa
     3781 ttcactgcct gctgataaga cgcatccacc agataataga tttgcgaatc cggcagcgaa
     3841 ccgtcgagat acacggtgct ccagtgcgct ttattcagat ggcggctagg acgcacatcg
     3901 ctgtgctgct gacgtagcag ctccgccagc tccgggctgg ttttcagcga aacagctggg
     3961 cgattttcta cttctttcac catcgcaaac agtacatctt ccactttgat ctgcgtcgct
     4021 ttccagtcgt tatgcacgct ctgttctgcg cttggttttg ccatgcaata ttgtagcaac
     4081 tccgaaatgg tcatttttta ctccccttgt agtgtcgcga tgatgcgacg cgatccgccg
     4141 tggatgcgat gttcccccag ccagatgcct tgccaagtgc cggtctgaat acgcccttta
     4201 tgtaccggca atacaagcga tgttcccagc attgaggatt tgatatgaga aggcatatcg
     4261 tctgctccct catagtcatg ctcataattt ccgttgtcgg gaacggtgcg gaggaaaaaa
     4321 cactccatgt cgtggcgtac ggtgggatcg cagttctcat taagtgtcag agaggcggag
     4381 gtatgttgca gcaacagatg cagtaagccg atgttaacgc gcggcatatc agccagctga
     4441 ttcagaattt catccgttac cagatgaaac ccacgagatt tggcgctaag cgtgagcgtc
     4501 ttttgatacc acatgtgctg ctccttgata aaactctctt aatcagtttg cagcaagaca
     4561 gcgaaaggat aaaggtgtga ttaaaaaaac agcattcggg agagcatcac tctctcccgc
     4621 tctgtcagta ttctgaattg acgatcacct cttcaccaaa cgcacccgct tgtggcaagg
     4681 gtttgtaggt agagttggag gcgcgcagaa tgcggatacc acgagcgccg acatcgcgtg
     4741 cggcggtaat atcgttatcg gaatcgccat aaaaaattcg gatattttta tcctgcagcc
     4801 attgcgattt tgtattttgc cctggtttat cgcccgcaaa gattaccgga ttcatgttgg
     4861 tggcaggaat atgaaaatta tccgccagcg tttttgaaac cgtttctgtt ttcgtcgggc
     4921 tacgaccagt cacaaagaag atcgcgtcac cgcggcgtac atgcatatca atcagctggc
     4981 gagcgacctc ttttggaatg ctgaattcat cccagccatt attcattttt tcccagaaca
     5041 caggattttt cagataatct tcgctttctg gcgagaaggt ttttttgccg cgccagaacc
     5101 ccggactgga aaaaagtacc gtgtcatcga tatcaaaccc caccgccatt ggcggacgcc
     5161 ctgcgaggct attttcaatt tgtgcgaccg aaacccaatg aattggtgcc tgttcagcaa
     5221 gcctggcaac gttagtccca gggttaagcg gtgaaggaga tgaggccagg gcaacagcgg
     5281 aactgtttag agcgaacaat aagcaaacgg cactgattgc ctgtgtgatc ttgcgcatat
     5341 ttttccctaa atagtcagta tgttgaaact ttttattgtg agatttgttg caaaaaccat
     5401 ctgaccataa cgccagctgc cggggaaagg aaggattatc tgcggttttt gtgatgaaaa
     5461 gcaggatgat tatatttaac ccagtaaata cgttcctgtc tttttttgta taaaaaatgt
     5521 ttattactcg atgtaaggat acattaattt tacgtttatt atgatgaatc ctcggcaaat
     5581 gagcgaaatt tgatagaaca tttatctgct gtacgattta atgaacttaa tgatacagca
     5641 ttacaaaaac aatcgaagtt tataaagatg atttctgatt gaccagcccc ctaccctaca
     5701 atggtgatta attttattac tcgcgatgga gatgatgata tgaatatatc atatgtgaat
     5761 agcaacaaaa cgacatcttt acctgttgag ctagatgcac taaataataa agatatctct
     5821 tatgccaaag atttcttttt atacatagaa acacagttaa aaattgcaaa agatttttgt
     5881 agacctggag aagaagtatc aagttctatt gcaagtaaag tttttcacgc atttattgat
     5941 ttagttaaca aaatcagggg taagaaagac tttatgtata tttgcacgct ttgctgtttt
     6001 gccgaggagg ttaaaggtga ttattctcat tacaggacct ttttatttga tattggtaat
     6061 caatataagg ttaaacttac acaaagcgga aaaaagagtt ctctttaaca ttagaattta
     6121 acgatactat aattgaatct cagaaagtca caggcaataa ggcaaagcat attcttgagg
     6181 atatagaaat attctatcgt aacaaacccg atacttatta ttaaatttaa taatggttag
     6241 atgtgcagaa attattactc cacacgaaaa aatagggaaa ggaattccag ataattggga
     6301 taaacttaac aataaaagaa ctgacaacgt tttattatct ctcacgtaga acaacggcat
     6361 caaaaaaatc atctacaaag aaatagtgaa agcgacgaca gtttaatttc actgcaggct
     6421 gggtaactcc agcctgcttt cctgcattac atcaccgcag caaacgcctt tgccacacgt
     6481 tgtacatttg ccgtatttaa cccggcgaca cacatgcgac cgctggcgat gagatagaca
     6541 ccaaattctt cacgtagtcg gtcaacctga gcggcactta aaccggtata actgaacatg
     6601 ccgcgctgat taagcagata atcgaaattg cgttctggca tctctgtgct taataccttc
     6661 accaattcct gacgcattgc cagaatgcga gtacgcatct cttctacttc cgccagccag
     6721 ctggctttca atgcctcgtc attcagcact gcagccacca cctgcgcacc aaaattcggc
     6781 gggctggagt agttgcggcg aactgttgct ttcaattgcc ccagtacgcg gccagcggct
     6841 tcggcatctt cacacagaac agaaagtccg ccgacgcgct cgccgtaaag ggagaaaatt
     6901 ttcgagaacg aattgctcac cagagcgggt aatccagcac tggcaatggc gcgaatagcg
     6961 taggcatcct cttccatacc ggcactaaat ccttgatagg caatatcgag gaatgggata
     7021 agctcgcggg ctttgagaat ttcaatcacc gcatcccact gatcattagt gagatcggca
     7081 cccgttgggt tgtggcaaca tggatgcagc aacacaatac tgcgggcagg taatgttttc
     7141 agcgtcgcca acaggtcatt aaagcgcacg ctgttagtca cttcgtcata ccaggggtaa
     7201 gtactcactt cgaatccagc cccggcgaat attgctacgt ggttttccca agtaggatcg
     7261 ctgacccaga cgcctgattc cgggaagtag cgtttcagga aatccgcgcc cactttcaat
     7321 gcccctgagc cgccaagggt ttgaatggtt gctacgcgct gttgttgcag taccggatgg
     7381 tccgcaccaa acagcagcgg cgcaatggca tggcgatagc tgttaagccc ttccatcggt
     7441 aaataaagcg aagcgccatg gggctgcgca ttcaggcgcg cttccgcctc cgccacggct
     7501 ttcaattgtg gaataattcc gtcttcgttg tagtacagac cgatacttaa attcactttg
     7561 tcgctgcgag ggtcttcttt aaaacgctcc ataagcgtaa gaatcgggtc gccagcgtag
     7621 gcgtcaactt tttgaaacac gcgatggttc tccaggttta cgggcaggtg gttaaaacac
     7681 aataaaccgg aagaaggcga agatcgagtg gatgttcagg agcgaacggc aattagcaac
     7741 agagtgagac taatgataaa cgtacatccg ccagaggcac gacctttata agagcatgaa
     7801 agaacatcaa cttattgaat ttttaggatt ttattggccg gataaggcat tcacgccgca
     7861 tccggcacag acaatcaaat attacagaac gattaatcca cgtatttcat cgcgaccctt
     7921 gaagtcaggc gcgtaataag ttcgtaagcg cttactttcg tcatttcagc gatacgttct
     7981 acgggcaaac cttcgcccca taaaatgacc ggatccccgg ctttgtcctg cgcctgtgga
     8041 cctaagtcta cgcagatcat atccatcgcc acgcgcccga caatcggtac ttcgcgaccg
     8101 ttcaccagca ctggcgtacc ggacggcgcg gcgcgcggat aaccatcgcc atagcccatc
     8161 gcgactacgc caagacgggt atcacgttcg cttacccagg ttccaccata accaacaggc
     8221 tctccggctt tatgctcacg cacggcaatc aggctggagg ttagtgacat cactggctga
     8281 cagccaaaat cggcaccggt ggagcgatct tccagcggcg agacgccata aagaatgatg
     8341 cccgggcgca cccagtcaaa atgcgactgt ggccacagca gaatgccacc cgacgcggca
     8401 atggaacgtt gaccaggttt gccttcgcaa aaggtattaa agatagcgag ttgtttctcg
     8461 gttgcgccac attttggttc atccgcgcgc gcaaaatggc tgacgatatt caccggctga
     8521 cgaacgtttt tgcactgggt caggcgatga taaaacgcct cagcctgttc cggccttacg
     8581 cccagacggt gcataccggt atcgagtttc atccagacgg taaccggctc gtccaagcta
     8641 gcctcttcca gcgcagccag ctgttcttcg ttatgcacgg cggtatgaaa atgttgcgca
     8701 gaaatcgtcg gcagatctct ggcatcaaaa aagccttcga gtaacagtac aggtttggtg
     8761 attccccccg cacgcagtcg cagagcttct tcgagacggg ctacgccaaa ggcgtcagca
     8821 tcggggagcg ttcgcgcggt ctcaagaaga ccgtgaccat aagcgttcgc tttcaccacc
     8881 gcaaccattt tactggcagg tgccagttca cgaagacgtt gcaggttgtg tcgcagagcg
     8941 cggcggttaa tcacaacagt tgccgcttgc atttgtgttc cttgataatt gtttgcttta
     9001 attacctaat tcataaaata attattattc gtcgtcgtac tgcggccccg catagttgtc
     9061 gaagcgcgac cattgaccgt taaaggtcag gcgtaccgtc ccgattgggc cgttacgttg
     9121 tttaccgata ataatttccg cgatgccttt taaatcactg ttttcgtgat acacctcatc
     9181 acgatagata aacatgatca agtccgcatc ctgctcgata gagccagatt cacgcaggtc
     9241 ggagttgacc ggacgtttgt cggcacgttg ttccagagaa cggttcaact gggacagcgc
     9301 caccaccggc acgttcagtt ctttcgccag tgctttcagc gagcgggaga tttctgcaat
     9361 ttccagcgta cggttatcgg aaagcgccgg tacgcgcatc agttgcaggt agtcgatcat
     9421 gataagcccg atgccgccgt gttcacgggc aatacggcgt gcgcgggaac gcacttccgt
     9481 tggcgtcagg ccggaagagt catcgatata gatattgcgt ttttcgagca aaatacccat
     9541 ggtgccggaa atgcgcgccc agtcttcatc atcgagctgc ccggtacgga ttttagtctg
     9601 gtcaacgcgc gacagcgacg ccagagaacg catcatgatc tgttctgatg gcatctccag
     9661 cgagaagata agtactggtt tatcctgcaa cattgccgcg ttttcgacga ggttcatcgc
     9721 aaatgttgtt ttacccatcg acggacgcgc ggcgacgatg atcaaatccg acggctgcaa
     9781 gccagcggtt tttttgttga gatcgtcata accggtgttt accccggtaa cgccatcgtg
     9841 tggctgctga aacaactgct caatacgcgc cacggttgcg tcgagcacat cggcgatgtt
     9901 cttcggccct tcgtctttgt ttgcacgact ttcggcaatt ttaaagacgc gggattcagc
     9961 aaggtccagc agatcttcgc tggtacgccc ctgcggatca aaaccggctt cagcaatctc
    10021 attcgcaacc gagatcatct cacggacaac ggcacgttca cgcacgatgt ccgcataagc
    10081 actgatgttc gccgcacttg gcgtattttt tgacagctct gccagataag caaaaccacc
    10141 gacgctatcg agttgcccct ggcgttccaa cgattccgca agggtaatca gatcgatagg
    10201 actaccgctt tcctgcaaac gcgccatttc agtaaagata tgacggtgtg ggcgggtgta
    10261 aaaatcgtct gctaccacac gctcggctac atcatcccag cgttcgttat ccagcattaa
    10321 accgcccaac accgactgct ccgcttcgat cgagtgcgga ggcactttca gcccggcaac
    10381 ttgtggatcg cgttcgcggg gttcagcctg ctgtttgttg aagggtttat ttcctgccat
    10441 agtgaatgga gttaccgaaa taaagaatgg gtcgaaactt taccatatga agcagaccct
    10501 gacgatacgt tctggaggac acatggcaac acgaattgaa tttcacaagc acggtggccc
    10561 ggaagtactt caagccgtag agttcactcc tgccgatccg gcggagaatg aaatccaggt
    10621 cgaaaataaa gccatcggca tcaattttat cgacacgtat atccgcagcg gcctttaccc
    10681 gccgccatcg ctacccagcg gattaggtac cgaagcggca ggcatcgtga gtaaagtcgg
    10741 cagtggtgta aagcatatta aggctggcga tcatgtagtc tatgcgcagt cggcgttagg
    10801 cgcttacagc tctgtgcata acattaatgc ggataaagcg gcgattctgc ctgcggcaat
    10861 ttcttttgag caagctgcgg catccttcct gaaaggctta acggtttatt atctgctgcg
    10921 caaaacctat gaaattaaac ccgatgaaca gttcctgttc cacgcagcgg ctggcggcat
    10981 tggcttaatt gcctgccagt gggcaaaagc cctgggcgcg aaactcatcg gcaccgtagg
    11041 aaccgcgcaa aaagcgcaga gcgcgctaaa agccggcgcg tggcaggtta ttaactatcg
    11101 tgaagagaat ctggtcgagc gattgaaaga gatcaccggc ggtaagaaag tgcgcgtagt
    11161 gtacgattcc gtgggcagag acacctggga acggtcgctg gattgcctgc aacgccgcgg
    11221 cttaatggtc agttttggca actcatcagg tgcggttacc ggtgtgaact taggcattct
    11281 caatcaaaaa ggctcgttgt atgtgacacg cccttccctg caaggctata tcaccacgcg
    11341 ggaggaatta accgaggcca gtaatgaact gttctctttg attgccagcg gtgtgattaa
    11401 ggtcgatgtc gccgagcagc agaaatatcc gctgaaggat gcgcagcgtg cgcatgagat
    11461 tctggaaagc cgggcgacgc aaggttccag cctgctgatt ccataaaaga aatagggctt
    11521 ccacctggga agccctttct ttttatagtt cggctgtatg tagggtacag cacgatgaat
    11581 ctgttagagg cgcaatagtg acagatttga ttatcaattc ctattttgtt ctaaggataa
    11641 aaccttaggt tgtgatcatc cgcacaatcc cttagtaacg ccagcggtca taacgctgat
    11701 atttcggcat ttttggtgct ttaatcgcct taataaccca caccaccgca atcgccagta
    11761 gtaaccacgg cagcaactta atcatcaatg ccagcatacc gccgaggaac ataatggccg
    11821 tcgccacaac cagcgcggcg ataatgccca gcaacgaaac gccggtgacc atcagcatga
    11881 caaaaaagcc aatcacaaaa agtagttcca gcatgatgct ctcccaaata tgaaatctct
    11941 tgctggcatt acaagaatca tgccaaaaat aatctattga tttaacagca aaacgccccg
    12001 cgacggtgcg cagggcgtgg tgaatttgac tactttttgg tgaaaagtta acgcttatcc
    12061 gccaccagtt tgagcgcgtg ttccagcacg ttaatgtcag cacccgcttt atgggcattt
    12121 tcacttaaat aacgccgcca ctgccgcgcg ccaggaatac cctggaacaa acccagcata
    12181 tgccggataa tatggccgag atacgtcccc tggctgagtt catgctcaat gtacggatac
    12241 atggcacgca ctactgccac cggatcggca tcggtatccg aggaaccaaa gatctcccgg
    12301 tctaccgccg ccagaatacc cggattctga tacgcctcgc gcccgaccat cacgccatcc
    12361 atatgttgca aatgcgcttt agcttcttcc agcgacttga taccaccgtt aatcgacatt
    12421 gtcagatgcg ggaagtcacg cttcagttga tacacacgcg gataatcgag cggcgggatt
    12481 tcacggtttt ctttcgggct taaccccgaa agccaggctt tacgtgcgtg gatgatgaac
    12541 atctcacact cgcctttgcc ggaaacggta ttgatgaaat cgcagagaaa ttcatagctg
    12601 tcctggtcgt cgatgccaat acgcgttttc accgtcaccg gaatcgacac cacatcgcgc
    12661 atcgctttca cgcagtcggc aaccagctgc gcattaccca tcagacacgc accaaacatg
    12721 ccgttctgca cccggtcaga cgggcagccg acattcaggt tgatctcatc atatccgcgc
    12781 gcttctgcca gctttgcaca ctgcgccagc gccgccggat cgctaccgcc cagttgcaac
    12841 gctaccggat gttcttcttc actgtacgcc agataatcac ctttaccgtg aattatcgcc
    12901 cctgtggtca ccatttcggt ataaagcaac gtattgcggg aaagcagacg caagaaatag
    12961 cggcaatgtc tgtccgtcca gtcgagcatt ggtgcaacac taaaccgacc actccagtga
    13021 acgtcagttt tttcaggcat tgcgctggtt tggttgattt tttgcatttc agaattaccg
    13081 tgcattttca aatgtagaga ttattttatc gatatatcat ggggttatgt tattcagctt
    13141 cactgttcag gaggctcaat agcggggtac tataccataa caacaggaag cgcctgtctc
    13201 attgcaaaag aaaattgaga tcctctcaag gcatgaagct ctcacgaagt gatggaaata
    13261 atcttattag ccgttaactt tgttaaggcc aatgataaac aatccaggtt cgacgataaa
    13321 taaaaaatca cacattaaac tctggtgata tatctccctg ctaatagcat tgatagagaa
    13381 aaaagaaccc aataaatatt gggtcctttt atataatgcc ttccatacta tcgaagaact
    13441 tcacatatta tttctccgat ttaaccccga acaagtcata aattaattta gaagtgtctg
    13501 taagtatttt aatctcttcc tttgaggttg ggtcaaacga cttcgcgaag ctaattaatt
    13561 gtggtgcagc atctcgcatt ttgcttaaaa tatcaggatc gagcgttcct tcattcacca
    13621 gatgtgacat cttatccaga tagccatcaa aattcatttc tcccccatct gccagacgct
    13681 tcatctctcc caggtacttc ttcatatcac gttgatataa tttttcaaac tgagctttca
    13741 ggtagctctc attattttca ttaatataca ctgtttctga taaatctgcg taggcactag
    13801 aatatgaaag tgaaattaac agcgaagcaa ataattttaa gctgttgtca tcatgttcct
    13861 ggcaggcatt aacaaacgtt aaaaaaccag aacctattgc ttgaaaatgt atattcgcta
    13921 aaggttcatt atccttagat tcctcataaa atagctgaac cgtggaggga aggccattct
    13981 tagtttctac atcaaaagta cattgttcaa tgggcattgc atctttatta tccccaggat
    14041 tacatatact attaatcgcg aaatgaaaaa tagcccggtc aacaactgat gcaataaagt
    14101 cacgatcgtt taaatcctca tcagtgcaat cgtcagtata cttattgacc cacattttga
    14161 gatcatttat gtgctcaatt aaaaacacat ttttttacgt gcatgccata aattatgcgt
    14221 gattcgttga ataaacaaat ctatttttgc aataattata tcccgatgac gcagcgctgc
    14281 ataatcaata gatttaccat atatattatt taatttacct ttgattgaac ctctattatc
    14341 caatttatct ttggttgcat ctttattttc gaattgattt gaattgagtg ttttattaaa
    14401 gtatccagtt gcaccgggta ttatcttcaa catgattcat ctaccttttt tgtcgtaccg
    14461 tgtcctcctt ataattcttt tgccatctgg tcactatcaa aaatcgctca taatttctga
    14521 actacaggta tgcgtttaaa cccagttaaa taaatagcga gttaacttga agcaatttat
    14581 ttaaaaatga attattaatt attatcgtgt tgataatggt acgataaggc aacacaacac
    14641 cttacctaca aaacagaaaa tcaacattac ggcaacaata agggttctcg gtgtttttcc
    14701 tggaacatgg taaagtaagg acattcttaa cccccacttt gaggtgcccg atggaaaaga
    14761 ccacaacgca ggagttatta gcgcaggctg aaaaaatctg cgcgcagcgt aatgtgcgcc
    14821 tgaccccaca gcgcctggaa gtgttgcgcc tgatgagtct gcaggatggc gctatcagcg
    14881 cttatgatct gcttgattta ctgcgcgaag ctgaaccgca agccaagccg ccaacggttt
    14941 atcgcgcgct ggattttctg cttgagcaag gttttgtgca taaggtggaa tccaccaaca
    15001 gttatgtgct ctgtcatctg ttcgaccagc ccacgcatac gtcagccatg tttatttgcg
    15061 atcgctgcgg cgcggtgaaa gaagagtgtg cagaaggcgt ggaagacatt atgcatacgc
    15121 tggcggcaaa aatggggttt gccctgcggc ataatgtgat tgaagcacat gggctctgtg
    15181 cggcatgcgt ggaagtggaa gcgtgtcgtc atcctgaaca gtgccagcat gatcactctg
    15241 tgcaggtgaa aaagaaaccg cgttaagagg gcgtacatcc ttgtacacgt cgggcaggag
    15301 ggattaatta ccagcgatat tcattgcggg tttcccaatc cacgacctct ttttctgcct
    15361 gatctttctg ataaccataa cgttcctgga ttttaccgac cagttgatca cgtttacctt
    15421 caatgatcgt catatcatca tcggtcagtt tgccccattg ctctttcact ttacctttaa
    15481 actgtttcca gttaccgccg gcttcatctt tattcataat caagacctca tcgttaggtt
    15541 gtgaatgaga gtacgttcac ttttcttctg aacgtgagat taagtgtagt cagcaatttg
    15601 gcttaggata atttattcag aatttttaac cgtcacgttg cggcaaacca ggtaccgttg
    15661 cgccagtgac gccgccagat agccgccaga gaaagcccgc gcaacgccag aaagacggtt
    15721 aatgccagcc acaaaccatg attacccagc cacggcagcg taaggagcgt cagcgcaaaa
    15781 cctgcggcgg ccaccgccat actgttacgc atttcggcgg cacgcgttgc gcctataaac
    15841 atgccgtcca gcagataaca ccagacgcca accaacggca aaatcacctg ccagataaga
    15901 tagcggtcag ccagctgctg aatctgggtt aacgacgtca gcaacgcaat gatgtgttcc
    15961 cctgccagca aataaaccac cgaaaatagt aacgctacaa tccccgactg gcggcacgct
    16021 gcccgccata catccagcaa ctggctaccg tcgcgcgcac cgtatgcctg accggagtgc
    16081 gcttcaaccg cgtaggcaaa accatcaagc gcataggcgg taaaggtgag tagcgtcatc
    16141 agaaccgcgt taacagcgat aatgtcactc cccagtcgcg caccaagtac ggtgatcgcg
    16201 ccgaaacaga gttgcaacaa cagcgaacgc agcatgatat cgcggttaag cgccagcaag
    16261 cgacggaagt ttcctcgcca ggcagttttc agcatttcgc cggaaattcc gcgtagtttg
    16321 aggattttac gcaccattag cagaccaatc agcaatgttg catattccgc aataaccgtc
    16381 gccagcgccg cgccctgcac gttcatatgc agccccatca ccagccagac atccagcaca
    16441 atgttgagga tattgccgac cactaacaaa attactggcg cacgggcata ttgcacgccg
    16501 agtaaccaac cgagtaatac cagattcgcc agcgacgccg gagcgcttaa ccagcggatt
    16561 tcaagaaagc gccgcgcctg ttccaggacc gcttcactac cgccaacaat atgcagcgcc
    16621 agatcgataa tcggcgtacg caacagcgca attaacgccc cggcccccaa cgccaacagc
    16681 aacggttgca ccagcgcacg ggctaatgcc tgaggatttt tggcaccata agcctgcgca
    16741 gtcagcccgg tggtgctcat gcgtaaaaac agcaacagca taaagagaaa gctggtcgcc
    16801 gttgcaccaa ccgccacgcc gcccaaataa accggactat caagatgacc aattaccgcc
    16861 gtatcgacca gtcccagcaa cggaacggtg atattggaga aaatcatggg taaggcgaga
    16921 tgccagagtg ctttatcaga tgaagtgagg aatgccatgc agacaagcct gatgaagaga
    16981 gatgaaaaac aaaccgcgac gccaagcggc atcgcggtct cagagatatg ttacagccag
    17041 tcgccgttgc gaataacccc aaccgccagc ccttcaatgg tgaagctctg ctgacgaagg
    17101 tcaacgacaa ttggtttaaa ctcgctattt tctggcaaca gttcgacttt attgccctgt
    17161 tttttcaggc gcttaacggt aacttcgtca tcaatacgtg cgacaacgac ctgaccgtta
    17221 cgtacatcct gagttttatg cactgccagc aagtcgccat ccataatgcc gatatctttc
    17281 atcgacatcc cgctgacgcg cagcaggaaa tcagcattcg gcttgaacaa ggaaggatcg
    17341 acctgataat gaccttcaat atgctgttgc gccagaagcg gttcaccggc agccacacga
    17401 cctaccagcg gcaacccttc ttcctcttcc tgcaacagac gaatcccgcg tgatgcgccg
    17461 gaaacaattt caataacgcc tttgcgtgcc agcgccttca gatgttcttc agccgcgttt
    17521 ggggaacgga accccaaacg ctgcgcgatt tccgcacgcg tcggcggcat acctgtctgg
    17581 ctgatgtgat cacggatgag atcaaacacc tcttgttgcc tggccgttaa cgctttcatt
    17641 ccgccccctg ggtgtatata cagttatact gtgagtatat acagcaaaag gcgattttgg
    17701 aaccataaac tgcacaataa accagagatt tatcgaaatc tggaagggtt atccaaaatg
    17761 cgaccataac aggatgcacc aggtaatcac ggcgacgata atggcaatca gcaccgcggc
    17821 ggatcccata tcttttgcgc gtccggaaag ctcatggtat tcagagccaa ttcggtcaac
    17881 cactgcttcg atggcgctat tgaggatttc cacaatcatc accagcatca cggagctgat
    17941 aagcagcacg cgggtaattg cgtccacatc cagccagcag gcgatgacca ccgccaacaa
    18001 taccgctacg ccttcctgac ggaatgccgc ttcgttgatc catgcagcgc gtaaaccttt
    18061 ccaggaatag ccagcagctt tgataattcg ggtgaatcca gttgtattat tggccattga
    18121 aagaaccttt ttacattatg agcgtcaata tcagtgtacc gttaaatccg atgattcggg
    18181 tttgcgtaac gccgcgcgaa acatgagcgg ataccacaga atttcccatg actttctgct
    18241 atccttgccg cgcatttgca ttattaacca gaggctttac atcgtttatg tccggctggc
    18301 cacgaattta ctacaaatta ctgaatttac cattaagcat cctggtaaaa agcaagtcta
    18361 ttccggcaga tcctgccccg gaactggggc tggatacctc tcgtccaatt atgtacgttt
    18421 taccgtacaa ctcgaaagca gatttgctga cgttgcgcgc ccagtgtctg gcacatgatt
    18481 tgcctgaccc gttagagccg ctggaaatcg acggcacgct actgccgcgc tatgtgttca
    18541 ttcacggcgg gccgcgtgta ttcacctatt acacgccgaa agaagagtct attaagcttt
    18601 tccacgacta tctcgatttg caccgtagca acccaaatct ggatgtgcag atggtgccag
    18661 tatcggtgat gtttggtcgc gcgccggggc gtgaaaaagg cgaagtgaac ccgccgctgc
    18721 gtatgcttaa cggcgtacag aaatttttcg ctgtactgtg gctcggtcgc gacagttttg
    18781 tgcgtttctc gccgtcagtg tcgctgcgcc gtatggcgga tgaacacggc acggataaaa
    18841 ctatcgctca gaaactggcg cgcgtggcgc gtatgcactt tgcccgtcaa cgtctggctg
    18901 ccgtaggccc acgtctgcct gctcgtcagg atctgtttaa taagctgctc gcctcccgcg
    18961 ccattgccaa agcggtagaa gatgaagcgc gcagcaaaaa aatctcccat gaaaaagcgc
    19021 agcagaacgc gattgcgctg atggaagaga ttgcggcgaa tttctcttac gagatgatcc
    19081 gcctgactga ccgcattctg ggcttcacct ggaaccgatt ttatcagggc atcaacgtcc
    19141 ataacgccga gcgcgttcgc cagctggccc acgacggtca tgagctggtg tatgtgcctt
    19201 gccaccgcag tcacatggac tacctgctac tttcttacgt gctgtaccac caggggctgg
    19261 tgccgccgca tatcgccgcc gggatcaacc tgaacttctg gccggccggg ccgattttcc
    19321 gccgtctggg ggcgttcttt attcgccgta cttttaaagg caataaactt tattccaccg
    19381 ttttccgtga gtatctcggc gaactgttca gccgtggtta ttccgtcgag tacttcgtgg
    19441 aaggcggtcg ttcccgcacg gggcgtttgc tggatccgaa aaccggtacg ctgtcgatga
    19501 ccattcaggc gatgctgcgt ggcggcacgc gtccgattac gctgattccg atctatatcg
    19561 gttatgagca cgtcatggaa gtgggtactt acgccaaaga actgcgcggc gcgacgaaag
    19621 agaaagagag cctgccgcag atgctgcgcg gtttaagcaa gctgcgtaat ctcggtcagg
    19681 gttacgtcaa cttcggtgaa ccaatgccgt tgatgaccta ccttaaccag cacgtaccag
    19741 actggcgtga atctatcgat cccatcgaag cggtgcgtcc ggcctggtta acgccgacgg
    19801 tcaataatat tgctgccgat ctgatggtac gcattaacaa cgcaggcgcg gcaaacgcca
    19861 tgaacctgtg ctgtactgcg ctactggcat cacgtcagcg ctcactcacc cgcgagcagt
    19921 taaccgagca actcaactgc tacctggatc tgatgcgcaa cgtaccttac tccacggact
    19981 ctaccgttcc ttcagccagc gccagcgagc ttatcgatca cgcgctgcaa atgaacaagt
    20041 ttgaagtcga gaaagacact atcggcgaca tcatcattct gccacgcgag caagcggtgc
    20101 tgatgaccta ctatcgcaac aacattgcgc atatgttggt gctgccttcg ctgatggcgg
    20161 caattgtcac ccagcatcgc cacatctccc gcgacgtatt gatggagcac gtcaatgtgc
    20221 tttacccaat gctgaaagcg gagctgttcc tgcgctggga tcgcgacgag ttgccggacg
    20281 ttattgatgc gctggcaaat gagatgcaac gtcaggggct gattaccctg caagatgatg
    20341 agttgcatat caacccggcg cattctcgca ccctacagct gctggccgca ggcgcgcgcg
    20401 aaacgctgca acgttatgcc atcaccttct ggttgttgag tgccaacccg tcgatcaacc
    20461 gcggtacgct ggagaaagag agccgcaccg ttgcgcaacg tctctccgtg ctgcacggca
    20521 tcaacgcgcc ggagttcttc gacaaggcgg tgttcagttc tctggtgctg acgctgcgtg
    20581 atgaagggta tatcagcgat agcggcgatg ccgaaccagc agaaacgatg aaggtttatc
    20641 agttgctggc ggagttgatt acatcagacg tgcgtttgac gattgagagt gcgacgcagg
    20701 gcgaagggta atcagagaga attgccggat gcggcgaaaa cgccttatcc ggcctaccat
    20761 gacctgcaaa ttcaataaat tgcgattcac cgggtaggcc tgagaagcgc aacgcatcag
    20821 gcaacccaga agaaagccgg atgatcatcc ggctttttta catcatcaga aatgccagta
    20881 actcattgcc agtcctaaaa acagtaccag accaacatag ttattattca taaatgcttt
    20941 aaagcaggct tcacgctcgc ggttggcaat cagtttttgt tgataaacaa acagcgcgcc
    21001 agccaccaca attgaccagt aatatcccca gcctaagcca tttaactcac cgatgatcgc
    21061 catcagtgcc agtacgccaa tctgcaaaat accaataatc aatttatcgt attggccgaa
    21121 caggattgcc gtggatttaa tgccaatctt cacatcatca tcgcggtcaa ccatcgcata
    21181 ctgcgtgtcg taagccaccg cccagagaat attggcgagg aacattaacc agcaactcaa
    21241 tggcaccgac tcactcacag cggcaaaagc cattggaatc gaccagccaa acgccgcgcc
    21301 cagcaccact tgcgggagat gggtataccg cttcataaac gggtacaccc acgccaatgc
    21361 cagagcggca atcgacaaca gaatggtcat cgtattcagc gtcagaacca gtaaaaacga
    21421 aatcagtacc agcactacaa acagcgcgcg cgcctctttc tctgttaccg cgccgctggg
    21481 aagtggtcgg ttcgccgtgc gcttaacatg accatcaaac ttgcgatcag cataatcatt
    21541 caccacgcaa ccagcagcgc gcatcagcca gacacccgcg acaaacaccg ccatgatcca
    21601 gagctgagga acgcccggtg tcgccaccca caacgcccat aatgttggcc agagcagcag
    21661 taacgcgcca attggcttat ccgtacgcat taagcgatga aacgccagca gcttattctg
    21721 cgtcagactc cactccatat ttttttcctc ttagtacaac ggtgacgccg gtaaaaacag
    21781 ttctgttagc aacagcggtt taccgcttaa tcgcaggcgg gaacgtcgcc cccacagtcc
    21841 ggcatcacgg cctatctcga taaaatcccg ggttagtgtc gatgatgtga acagatagcg
    21901 tcctaacggc gttttaccca atttttgtaa cgctagttcc ggcccgctta atgttgatac
    21961 aggaacgacg gtacgaccgg caagccacgg ttcaccatcg gcacataaca aaatttcacg
    22021 taaccagtaa cgagactctt tcggcagcag cggcagttct tcggggattt cattctgctc
    22081 gacaaacccc tcgcggatca tcgtcacact caccgttttt ccctgctgtt caaaacgttt
    22141 tgtcatggaa tcctccagca acagccagtc gagcagttgc ggatccaggg cagggatctc
    22201 tttaaaatag cgcagcgcac gcagttgcgt taacgcgggg tgtgacatgc cgaactctcc
    22261 gttacataac gtaaaggcat tgtatcgcag aaaagtgttt acgggagatg taaagcaaat
    22321 taagtgatct aatgcgcaac aaaaacgcaa caatcgcatg aagtggtaaa acgctgttga
    22381 accttacgct ttgccgatgc ccgtgctggc gagggcaacc ataggggcat caccgcccat
    22441 gatgctagga acggtgatgt tttcctttac ttaaatacct ttattcttta tacgagtagc
    22501 tcgccaactc gcagtaaaaa ttgggaaaag cgcatattta ccatgagatt tggagctgaa
    22561 aagtgtttcc tgagtaagat taataggcaa catcttatgt ctggatttat agatacacgt
    22621 ggaattccaa ttggtatctt ttgtaggatt caacatatca tgtaagcgct gggagcttac
    22681 caccataaca ggcccatcat cgacagagga gaacaggata aatatattat cgtttcgtgt
    22741 taatttatta gtgttaaagg ttcttaactt ttcttcagtt tctataaata taggtgaaac
    22801 atcatcacta cgaatccact ctttataaag ataccaggca tttgctttga tcatatcatc
    22861 cgttgatcgt aaggcctgat cgataagttg gataaaaaaa ttgttatttt tatcaaccag
    22921 ccctttattc atattaaatc tgttaataaa atggatcatc tgttgggtat cgaaattcac
    22981 cttactgaat cgatgttgac gcataaaatt atcgaacttg tcatcttccc cagaatgtat
    23041 ataactacgt ataattggat cttttatata aggacaatgg ctaaatgttt tcttcatatt
    23101 ctcaataaag tttttattaa caatattgag aatttttaat tgcatcacca gttttactgc
    23161 cagaacggat ttcaatatca ttgcattatc tggcaatgta tcaatcattt ttataatgtt
    23221 attgatataa ccatcgagat cttcattccc aaatatatct gaataatcaa tagttaattc
    23281 caattgttct gtaatgcaat cttaaaaatt ccctgtcgac attcgcacat gatcaaacat
    23341 aacagctgtc aaattcgatt taataaatga tatttctttg gcagacagaa actctgctct
    23401 ggtgaagtgc gaagcgtaaa gataacaatt atcgaagttt gaatttctaa tctgacattc
    23461 gcgaaaatta ctatgaccaa gttgactctg cgagaagtca actgtatcaa gaatattttg
    23521 gctaaaatca acacattcgc cactcgtttt gagaaaaatc acattgtgta aacgtcgaca
    23581 tttaaatatg acattatcaa ctgttgcctt agtaaaattg caattctcaa tttctttaca
    23641 ttcgaaagtt acatgttgta gatttgatga taaaaaagac accttagaaa taacgtcacc
    23701 tttaaaatga caatttgtta aatcaaaatc agacaggtcg atatacccct gcgcatcttt
    23761 atccatcaga aaattacaag cgcattctat tttttcattt ctgtagaatt ttacacttat
    23821 agtatcatta ctaattgaga cttcacgtaa catatttatt gcatgatttt tttcgttccg
    23881 ttgttttata tttaaaaata tatcactgat ataaataaag taatctctgt ttaaacctgt
    23941 caccccattg aaattacgta gtaaatcaag atctactata ttatttcctt gcaccacatc
    24001 atgtttcatc aaagagttat tgccgccaga atttgtgata tgacctatca taaaacctct
    24061 gtaatacaga aataaaataa cctggaaaag tagcctgagc gtccagacac taccggcgaa
    24121 aggaaaagaa tctctgataa tgcattgaaa taatggacat tcttagtgaa gaagaagtga
    24181 agtactataa aaaatcgctc atatcccagg aaggaagcat aaaaaaaggt gcgccaggag
    24241 acgcaccagt tgttgcaaaa tcagcactgt ggggcgtaat taccccttgc cttttacact
    24301 gctgataaag gtggaacggg cagatgtcga tcccaggcgt tcagcttcat caagcagttt
    24361 taacgcctta tcaacatcac ctttcgcgac agcgtttttg atcgcggtat taaaataact
    24421 ttccgtgtcg ttgagcatcg gctcgctttt cttcaccggc accggagcag gtgcagccac
    24481 agctggtgcc gccgtgttgc ctaccgtaac cggagctgga gcggaagaac caaatagcgg
    24541 tcctaccaac acgctggagc tggagttcgt tttgactttc agtttcagta agccatcgct
    24601 ggtatgacga gcaaccggat cgggaatatc cgggatcgag ttaccgacgc ctttggcata
    24661 ggctttagcc gggtcgagca gttgggtcgt ctgctggaga tctttttccg tggtaaagac
    24721 cagaacataa agtttttgct gccccaacgc cggtgtcagg cgcataacgc cttccagccg
    24781 atctgcactc atcacgccta gttcctggta ggtgaaataa ctgctgggga agaaggctga
    24841 tggggtcatg ttctgatcaa gaatcagcac gttcggcgca aaaacgctgg tttgtttgtt
    24901 cacttcgctg gtcagcgtca gggtcagttc gccaatgttt gccgggacgc tgtacgcagc
    24961 aaccggacca ctgatgccgg gaacgttcag ttgttggccg ccggtcgcca gttgggtggt
    25021 ctgggtttta gattgatcga ccggtgtcca ggtgagttgt tgcagcgcgg cagatggaat
    25081 ggctggcgcg tcgctggtgt tttgcggtac gtagttaaca tcggcaaggc taattccagg
    25141 cgcgcttgcc agtaaccctg ctgataaaca gagggcgatg agacttttat tcattttcat
    25201 tgttttcacc tcaaaatctg gagctcagcg gtagccaggc aatagcgcgc taaacccgat
    25261 aatcagaggg gctttcgccc ctttcagata atgacaacct gtttttatgc cggatgcggc
    25321 gtaaacgcct tatccggccc aggttttgct attaccacca gatttccatc tgggcaccga
    25381 aggtccactc gtcgctgtcg ccacgaccga agctgccgcc gttgaaatca gcaggaacgg
    25441 ctttgccgta gttcgggtta accttgctat cgccgttgta gtcgtaaccc catttctcat
    25501 cccacttggc gtaggttgcg aagacacgaa tagccgggcg tgaccagatg ctgtcgccag
    25561 cctgccattg ttgtgcgagg gtaattttgt actgattgtt cttgtcgccg gtgcgctggg
    25621 attcgacgtt gtcgtagccg atttccatca cggtgctcat gattggcgtc cacttgtaca
    25681 tcgggcgaat accaacggtc caccacttgg tgccgttgtc gttatcccag ttgatatcct
    25741 ggtacatacc cacgtacatc atgtcccagt tgtcgcccat ggagatcgca ccgtggtcga
    25801 ggatacgcag catgtgaccg ttgttgttga tattgtaggc aaatttttcg ttatcaaacg
    25861 cgacgccaga accctgcgac agacctttac cctgcgaggt catcgagtca gtagcgtact
    25921 gaacaacaaa cttgttaaag cccttcagga cactctgagt atgttcagca gtgaataacc
    25981 agccgtcttt cgatgcgcca tcaaccagac gatagttatc acgcaggttg gcacgaccgt
    26041 agtcgacacc cagttctaat gtgccgcccg ggttgatttc catctgcgct aaacgcacat
    26101 cgaaaacgtc gttcgcggtt tcgttggtat agtcataaat attgttgctg gcaaaagagg
    26161 aagaaccacc agcttcagag gagcgggttg ctgccagaga gagtttaccg aagccaacat
    26221 cgatgttttc cagaccggca ccaggaccag aaatatccca gtagtagaag tcgatcatat
    26281 gaacgtcatg acgttggtag aagcgcttac ctgcccagat ggtggaacct ggcagccatt
    26341 cgatcaggtt ttttccctgc acgtttgctt cacggaaggc cggatcggta gcttcccagt
    26401 cattctgttg cgcgacggaa taggccacgt tagtgtcgaa atagaagctc ttatcgccct
    26461 ctttccacac ttcctgaccc aattttaatt cagcataagt ttcacattcg ttgccaagac
    26521 ggtatttact ttgagcaccg gtagtctgga aacactgttg ttcaccgccg ctacctgtcc
    26581 agccaatacc ggaacgtgca tagccgtgga aatcaacagc cattgcctga gcagacatta
    26641 cgcccgctgc gacggcaacc gccagaggaa gtttgcgcag agtaatcatc attctatctc
    26701 ctgagtcatt gcttttcttt tttcacatca cctgtgacag gctttgtgtg ttttgtgggg
    26761 tgcttaaacg cccggctcct tatgcagtcg acgacatgca gtgccatcct cacggaacag
    26821 atggcaacgc tctggcggca ggccgatagc gaatgtggca ccttcttcta ccaacaccac
    26881 gtcgttctgg cggtacacca ggttttgacg aatggaaggg atctggatat agatttgggt
    26941 ttcgttgccg agttgctcga cgacctgaac ttcaccctca aggatgacgt cagcgatatc
    27001 actcggcagt agatgttccg ggcgaatacc cagcgacata ttggctccaa cctggacatc
    27061 acggctttca acaggcagcc agacttgttg acgatttggc atcggcagct ccacctgcac
    27121 ttgatcgatt gcggtggcgg tcacttttac cggcaggaag ttcatctttg gcgaaccgat
    27181 aaatccggcg acaaaacggt ctgccggata gtggtacagt tccagcggtt tcccaacctg
    27241 tgccacgcga ccggcgtcca gcaccacgat tttgtcggcc agcgtcatcg cttcgacctg
    27301 atcgtgggtg acgtaaatca ttgtgcggcc caggcgttta tgcagacggg agatttcgat
    27361 acgcatttgc acacgcagtg cagcatcgag gttggagagc ggttcatcga gcaaaaatac
    27421 gcttggctcg gccaccagcg tacggccaat cgccacgcgc tgacgctgac caccggagag
    27481 cgctttcggt ttgcgatcca gcaaatgcgc cagttgcagc acttccgcca cctggttgac
    27541 gcgttggtta atcacctctt tttttgcgcc agccagtttc aggccaaatg acatgttttc
    27601 tgctactgac aggtggggat agagcgcgta agactgaaac accataccaa cgccgcgttc
    27661 tgctggcgga gtgtcattca tccgtttctc accgatgaac aggtcgccgc tggtgatcgt
    27721 ctcaagcccg gcaatcatgc gcagtaaagt cgatttaccg cagccagacg gtccgacaaa
    27781 caccacgaat tcaccttcat ggatatcgag attgatatct ttcgatacca cgacctcgcc
    27841 ccaggctttc gttacatttt gcagctgtac gctcgccatg cccttctccc tttgtaacaa
    27901 cctgtcatcg acagcaacat tcatgatggg ctgactatgc gtcatcagga gatggcttaa
    27961 atcctccacc ccctggcttt tttatggggg aggaggcggg aggatgagaa cgcggcttct
    28021 gtgaactaaa ccgaggtcat gtaaggaatt tcgtgatgtt gcttgcaaaa atcgtggcga
    28081 ttttatgtgc gcatccccac attaccgcca attctgtaac agagatcaca caaagcaacg
    28141 gtggggcgta ggggcaagga ggatggaaag aggttgccgt ataaagaaac tagagtccgt
    28201 ttaggtgttt tcacgagcac ttcaccaaca aggaccatag attatgaaaa taaaaacagg
    28261 tgcacgcatc ctcgcattat ccgcattaac gacgatgatg ttttccgcct cggctctcgc
    28321 caaaatcgaa gaaggtaaac tggtaatctg gattaacggc gataaaggct ataacggtct
    28381 cgctgaagtc ggtaagaaat tcgagaaaga taccggaatt aaagtcaccg ttgagcatcc
    28441 ggataaactg gaagagaaat tccctcaggt tgcggcaact ggcgatggcc ctgacattat
    28501 cttctgggcg cacgaccgct ttggtggcta cgctcaatct ggcctgttgg ctgaaatcac
    28561 cccggacaaa gcgttccagg acaagctgta tccgtttaca tgggatgccg tacgttacaa
    28621 cggcaagctg attgcttacc caatcgctgt tgaagcgtta tcgctgattt ataacaaaga
    28681 cctgctgccg aacccgccaa aaacctggga agagatcccg gcgctggata aagaactgaa
    28741 agcgaaaggt aagagcgcgc tgatgttcaa cctgcaagaa ccgtacttca cctggccgct
    28801 gattgctgct gacgggggtt atgcgttcaa gtatgaaaac ggcaagtacg acattaaaga
    28861 cgtgggcgtg gataacgctg gcgcgaaagc gggtctgacc ttcctggttg acctgattaa
    28921 aaacaaacac atgaatgcag acaccgatta ctccatcgca gaagctgcct ttaataaagg
    28981 cgaaacagcg atgaccatca acggcccgtg ggcatggtcc aacatcgaca ccagcaaagt
    29041 gaattatggt gtaacggtac tgccgacctt caagggtcaa ccatctaaac cgttcgttgg
    29101 cgtgctgagc accggtatta acgccgccag tccgaacaaa gagctggcga aagagttcct
    29161 cgaaaactat ctgctgactg atgaaggtct ggaagcggtt aataaagaca aaccgctggg
    29221 tgccgtagcg ctgaagtctt acgaggaaga gttggcgaaa gatccacgta ttgccgccac
    29281 catggaaaac gcccagaaag gtgaaatcat gccgaacatc ccgcagatgt ccgctttctg
    29341 gtatgccgta cgtactgcgg tgatcaacgc cgccagcggt cgtcagactg tcgatgaagc
    29401 cctgaaagac gcgcagactc gtatcaccaa gtaatgctgt gaaatgccgg atgcggcgtg
    29461 aacgccttgt ccggcctaca aaactgaaac gcatgtaggc ctgataagac gcatcagtgt
    29521 cgcatcaggc agttgttgtc ggataaggcg tgaaagcctt atccgtcctg gaatgaggaa
    29581 gaaccccatg gatgtcatta aaaagaaaca ttggtggcaa agcgacgcgc tgaaatggtc
    29641 agtgctaggt ctgctcggcc tgctggtggg ttaccttgtt gttttaatgt acgcacaagg
    29701 ggaatacctg ttcgccatta ccacgctgat attgagttca gcggggctgt atattttcgc
    29761 caatcgtaaa gcctacgcct ggcgctatgt ttacccggga atggctggaa tgggattatt
    29821 cgtcctcttc cctctggtct gcaccatcgc cattgccttc accaactaca gcagcactaa
    29881 ccagctgact tttgaacgtg cgcaggaagt gttgttagat cgctcctggc aagcaggcaa
    29941 aacctataac tttggtcttt acccggcggg cgatgagtgg caactggcgc tcagcgacgg
    30001 cgaaaccggc aaaaattacc tctccgacgc gtttaaattt ggcggcgagc aaaaactgca
    30061 actgaaagaa acgaccgccc agcccgaagg cgaacgcgcg aatctgcgcg tgattaccca
    30121 gaatcgtcag gcgctgagtg acattaccgc cattctgccg gatggcaaca aagtgatgat
    30181 gagctccctg cgccagtttt ctggcacgca gccgctctac acactcgacg gtgacggcac
    30241 gttgacgaat aatcagagtg gcgtgaaata tcgtccgaat aaccaaattg gcttttacca
    30301 gtccattacc gccgacggca actgggggga tgaaaagcta agccccggtt acaccgtgac
    30361 caccggctgg aaaaacttta cccgcgtctt taccgacgaa ggcattcaga aaccgttcct
    30421 cgccattttc gtctggaccg tggtgttctc gctgatcact gtctttttaa cggtggcggt
    30481 cggcatggtt ctggcatgtc tggtgcagtg ggaagcgttg cgcggcaaag cggtctatcg
    30541 cgtcctgctg attctgccct acgcggtgcc atcgttcatt tcaatcttga ttttcaaagg
    30601 attgtttaac cagagcttcg gtgaaatcaa catgatgttg agcgcgctgt ttggcgtgaa
    30661 gcccgcctgg ttcagcgatc cgaccaccgc tcgcacgatg ctgattatcg tcaatacctg
    30721 gctgggttat ccgtacatga tgatcctctg catgggcttg ctgaaagcga ttccggacga
    30781 tttgtatgaa gcctcagcaa tggatggcgc aggtccgttc cagaacttct ttaagattac
    30841 gctgccgctg ctgattaaac cgctgacgcc gctgatgatc gccagcttcg cctttaactt
    30901 taacaacttc gtgctgattc agctgttaac caacggcggc ccggatcgtc ttggcacgac
    30961 cacgccagcc ggttataccg acctgcttgt taactacacc taccgtatcg cttttgaagg
    31021 cggcgggggt caggacttcg gtctggcggc agcaattgcc acgctgatct tcctgctggt
    31081 gggtgcgctg gcgatagtga acctgaaagc cacgcgaatg aagtttgatt aagggagata
    31141 acaaaaatgg caatggtcca accgaaatcg caaaaagcac gtttgtttat tactcacctg
    31201 ctactgctac tttttatcgc ggcgattatg ttcccgctgc tgatggtcgt cgctatctcg
    31261 ctgcgtcagg gaaacttcgc gaccggtagc ctgatcccgg agcaaatctc ctgggatcac
    31321 tggaaactgg cgttaggttt tagcgttgaa caggctgatg gtcgcattac gccaccgcca
    31381 ttcccggtac tgctgtggct gtggaactcg gtaaaggtcg ctgggatttc cgcgattggc
    31441 attgtggcgc tctccaccac ctgcgcctac gctttcgccc gtatgcgctt tccaggcaaa
    31501 gcgacgctgc tgaaaggaat gctgattttc cagatgttcc cggcagtact ttcactggtc
    31561 gcgttgtatg cgttgtttga tcgtctgggt gagtacattc cattcattgg cctgaatact
    31621 cacggcggcg taattttcgc gtatctgggt gggattgcgc tgcatgtctg gaccatcaaa
    31681 ggctatttcg aaaccatcga cagttcgctg gaagaagcgg ctgcgctgga tggtgcgaca
    31741 ccgtggcagg ccttccgcct tgtcctgttg ccgctgtcag taccgattct ggcggtggta
    31801 ttcatcctgt cgtttatcgc tgccattact gaagttccgg tcgcgtcgct gttactgcgt
    31861 gacgtaaaca gctacaccct ggccgtgggg atgcagcaat acctcaaccc gcaaaactac
    31921 ctgtggggtg actttgccgc cgctgccgtg atgtctgcat taccgatcac catcgtcttc
    31981 ttgctggctc aacgctggct ggtcaacggc ctgacggcag gtggtgtgaa aggttaaaga
    32041 tgttgttctg ccaatgttat gccgctgcac cctcaactta cgttatccca acttgtgact
    32101 attattcggc gctccacgga gcgccttttt ttctttcgtc tgcaatctga atcgttcgcc
    32161 ggttaatatt cccatcatag aacttattat ttttacgtta tttgttttcc cacttacgat
    32221 aattctcttt cgtgctctga gtcacggcaa tagtattgtt tcatcaattt ttgataatta
    32281 tcacaattaa gatcacagaa aagacattac gtaaacgcat tgtaaaaaat gataattgcc
    32341 ttaactgcct gacaattcca acatcaatgc actgataaaa gatcagaatg gtctaaggca
    32401 ggtctgaatg aatacccagt ataattccag ttatatattt tcgattacct tagtcgctac
    32461 attaggtggt ttattatttg gctacgatac cgccgttatt tccggtactg ttgagtcact
    32521 caataccgtc tttgttgctc cacaaaactt aagtgaatcc gctgccaact ccctgttagg
    32581 attttgcgta gccagcgctc tgattggttg catcatcggc ggtgccctcg gtggttattg
    32641 cagtaaccgc ttcggccgtc gtgattcact taagatttct gctgtcctgt tttttatttc
    32701 tggtgtaggt tctgcctggc cagaacttgg ttttacctct ataaacccgg acaacacagt
    32761 acctatttat ctggcaggtt atgtcccgga atttgttatt tatcgtatta ttggcggtat
    32821 tggcgttggt ttagcctcaa tgctttcacc aatgtatatt gcggaactgg ctccagctca
    32881 tattcgcgga aaactggttt catttaacca gtttgcgatt attttcgggc aacttttagt
    32941 ttactgcgta aactatttta ttgcccgttc cggtgatgcc agctggctga atactgacgg
    33001 caggcgttat atgtttgcct cggaatgtat ccctgcactg ctgttcttaa tgctgctgta
    33061 taccgtgcca gaaagtcctc gctggctgat gtcacgcggc aagcaagaac agacggaaag
    33121 tatcctgcgc aaaattatgg gcaacacgct tgcaactcag gcagtacagg aaattaaaca
    33181 ctccctggat catggccgca aaaccggtgg tcgtctgctg atgtttggcg tgggcgtgat
    33241 tgtaatcggc gttatgctct ccatcttcca gcaatttgtc ggcatcaatg tggtgctgta
    33301 ctacgcgccg gaagtgttca aaacgctggg ggccagcacg gatatcgcgc tgttgcagac
    33361 cattattgtc ggagttatca acctcacctt taccgtactg gcaattatga ctgtggataa
    33421 atttggtcgt aagccactgc aaattatcgg cgcactcgga atggcaatcg gtatgtttag
    33481 cctcggtacc gcgttttaca ctcaggcatc gggtattgtg gcgctactgt cgatgctgtt
    33541 ctatgttgcc gcctttgcca tgtcttgggg tccggtatgc tgggtactgc tgtcggaaat
    33601 cttcccgaat gctattcgtg gtaaagcgct ggcaatcgcg gtggcggccc agtggctggc
    33661 gaactacttc gtctcctgga ccttcccgat gatggacaaa aactcctggc tggtggccca
    33721 tttccacaac ggtttctcct actggattta cggttgtatg ggcgttctgg cagcactgtt
    33781 tatgtggaaa tttgtcccgg aaaccaaagg taaaaccctt gaggagctgg aagcgctctg
    33841 ggaaccggaa acgaagaaaa cacaacaaac tgctacgctg taatcttcct gtccagcacg
    33901 ccgcgccatt tcggcgtgct gtctttttac tcccgcttca gccgtttcga attacacagc
    33961 cacagggtga tcaccagtaa caggatcgca gccgagtaga tcaacacatc cagtggcgat
    34021 ttatgatcga cgatgatcaa gcgcacaatg gcggtgatcc caatatagac aaagtaacgt
    34081 agcgggaagt gaaaaccgga ctgaaagtac ttcacaatca gcgcgataaa ttcgaaatag
    34141 agaaagtaaa ccaccagtcc ttctaccagc tcatatttgc tggtttgttc tggcgcgaac
    34201 agcacatcag ccagatgcac cgtttctttg ccgaggaaga caaccaaaat caggccgagg
    34261 cacagcaggc caagattgag tacggtctgc aaaatggtgg agataaactc cacgcgcgga
    34321 cgagagagtg acgtcatagc agtacctcct tcacagtggc gagcttcctg tataacgcaa
    34381 aaatgtgacg gagatctata ttttttgtgt atgttttgtt caacctggat ttggagcgcg
    34441 cctccaccaa agccggatat acctacccgt aggccggata agccgcgcct ggcggcgcat
    34501 ccggcaagac aaaccgcacc aaagccggta attcaaaaat cacccattca ggtgattccc
    34561 agccaccagc aatgcgcgaa aaatcgacaa tgttcctctg gaacaaatga aacgtaacct
    34621 gataaaggtg gtaaaaatga agccttattt tgctgctttg atgttatcag tctctgttct
    34681 tcctgcttat gctggtccgc tcggtactgc tgataaagcc gacctaccgc aaagcaatgt
    34741 ctccagccca atgatggccc agtcgctcag gcaaccagac ctgcaaccga tatcgacgga
    34801 caggaagaca gaatgttttc gcctttacac accagatcgt aaaccggggg tgaactgcgt
    34861 tcctgatggc tcaacaggtc attaagaatg agggtgccag tgagtgcgaa aactggcacc
    34921 tttcccatga cttagcggaa atttacgcta cggtcgctgg tcatgtcata caactggaac
    34981 ttacgcccaa gttgctgacc accgtcacgc gtcagcggcg tccagccaat cgccgcacgg
    35041 ctgcgtgtcg ggccagacga gaagagatcc aacggtaccg agacatacac gcctttggtg
    35101 aagtcccctt cgccgtactc ctctttcgaa acgttggtga tcgtggcata gccacccacc
    35161 accacgccgc tgtcaaagcg tttagcgatc tccagcgtgc cgcctttatc ccctgccaga
    35221 tactgcccga cgctggcttt caccaacacg tcctgggcga aagatggcgt ccagtaggcg
    35281 gtcagatgtc cggttttcac gctgtagtcg gtgaatttca tcatatcttt tgcactacgc
    35341 cagtcgcgct gtttaacgta gttggcatcc agaccaaacg cccagttact gtccagcgga
    35401 cgatacaaca cttcggcccc cgcaccgcca aacatggttt cgagataacc tccatagacc
    35461 tgaccgtaga agccgttgcc cagatgctgg aagtagttgg cttgcaggtt attcacatag
    35521 acatcgttct gcacatactc gcgcacatgg gtacgaacgc gcggtaagtg cgagtcctgc
    35581 ggtggattgg tgtagttaaa cttgtcgtag ttgttggcaa gatttgcaaa caggctgccg
    35641 gtggtcagta gatggtccgt cagccacaaa tctgccgttc ccatcacgcc cagctgatac
    35701 atgtaaaagt tttccgggcc accgaccgac tggttcagca ccggatcgat atggaaatca
    35761 aagcgtgatt tgtcgatata ccagccctgc tcggtggact tcggaaccac tggctcgacg
    35821 cgtttgtgcg ccagcgtcgt ttcgtggccc aacggctctc cggcgagatg atttttcagg
    35881 ctggcgacat cggtttccgt cgtcacttgc ggcatgttaa ggcgattttc cgtaatgcgg
    35941 atcgttttga tcccatccgg cagatcgttc atcacgatcc gattagcacg gatgatccct
    36001 tcgcgcgaat cacgatattt cacctgctcg ccggtaacat acagcgtatc gcctttcgcc
    36061 tggatctgcg gatcggcaag tccggcgttg tatttcagca acgttaactg attcgccacc
    36121 accgaatgct gcaaaatggc atcctgcggc tgcggttgat attgcgggcg ggcgttatcg
    36181 atgtaggacg ggcgcagatc gttaaagttg gtgcgcagcg tgacgccaaa cataaaggtg
    36241 ttgccacgtt catagctaag gttaacgtcg gcccaatcgg taacgcgata aatcgccccg
    36301 acgttaaact tacttttttg ctccagcttc ccggcaaaat cctgctgata attattacct
    36361 tcatactcca gtttcagacg cagcggttgc cagggcgtct ggtattccac gccgccaaac
    36421 agtgaggcag gaccgtggaa catctggcta ccgtcgatag atcccgcctg tttgtagctg
    36481 ttatcgcgat agcaatattt atcactggct gaacagagcg gattttttac attaccgctg
    36541 gtgcccagat atccccagcc caggccgagc gtaaaatcga acggccccca ggctttgctg
    36601 gcaacaagat attccgcatc aaacagcccc gtaccgccga tatcccgcgc accaaccgcc
    36661 acttgcggca gccagtaact ctcttcccac aaacgcagtt tgagatcgaa ggctttatct
    36721 ttatacgttt gatcgccaga gaacgcttcg acgctgctgt actgccgggt gcgcacgtcg
    36781 gtgtagcgca gcgttgtttc cagccacggg aagagttgca ctgaagctga gtaataacgg
    36841 tactgatcgt tatcgcgata gttcagactc aactcccctt cccgtgccat acgcgcggtg
    36901 ggagtttgta ataatcctac gccaccgaaa tccgactgcg acggaccaat gggcgcagga
    36961 tatgtttcgc cgtagcaagc tgtgctaatg cccagcgcca gtaagctaag cagatgtctt
    37021 tttttcatta ttggggtatc cgctgcgtca gagtctgaag aatgtcggcg ttaagggcat
    37081 caggcgtctc actccagacg gaatctgcga ggccaacata aataatgctg ccgggcatcg
    37141 gctctacgtg acgcttgttc cagtaagcca ccggcgcttt ttgcgtgcgt ccgtccgggt
    37201 aaacgaccca cgcgtagctg cgatccgcac cgctgagcag gctctggtca gagagatagc
    37261 tcgccacgtc gcgacctgga gtgaatggct gattgccagg atggctgata agcccgaaca
    37321 acgtgaccgt ggacggcggt ggtccgaccc acagcgtgta gttgccctgc aacggcgggt
    37381 taccgcgttc ggcaacgcgg acgatatcgg gatcaagatt gattttttgc ctgcctgtca
    37441 ctttcaatgc ctggatttgc tggcgtaagg cgttaatggc ggcggcatcg tcggtgctgg
    37501 aatctgcgcc ctgttctgcc agccgtgtca gcaacgcctg ctgctgacgc aatgctgccg
    37561 ccgttgccag ctcttcgcta atcaccgcac caggccacca gctgtttgcc agtcgaggtt
    37621 gccctaccag atcgattaaa tgctctgcgc ccgttagcgt tttggcctca ctgctgccat
    37681 tgctgaacac cttgacggtt cctgccgcga agaccgatga cgctcccaca ctcaaaagca
    37741 acgcgacaat agtttgttta atcattgttt tgccgccttg atgagagtgg ttttcaccgg
    37801 gaaataatcc gcgccgagat actgttccga ctggcgaatt tgcccttcgc tgtcgatcca
    37861 gtagcggtta tgccagcgcg cctggtcggt ggagacttct tcgtccagca cgcgaaccag
    37921 ggtttcatcg ctgccgacct tgacggtatc ggtgccatcc catttgaaga ctgagcgtgc
    37981 ggtggcgtag cgtacctgct ggtactcggt ccagcccatc gtgcgcgtcc atgttgcacc
    38041 atcaacaatt tgtgcgggtt taatcagcgg gtcggcggca aggttattca cttcaatcag
    38101 gttgtcgccg ccaagcaaag tcttcaccag acgaccatgt tgtgtgacaa gagtggcttg
    38161 atcctgagtg acccattttt gttgtccgtc ttcagcgaag gccagcacca caaacaattg
    38221 cggcccgcca ttaagctgca tgtactggct ggcgtagggc atattttgaa tatcatcgtc
    38281 cgtcagctgt acgcctggcg tgccgaacag actgtcccac agtgaattgc ccagctcttt
    38341 agtcgtggct gaacaggcct gtaatagcag gcagataaga atgagtgcag gtcgcttcac
    38401 gactcttctc cagaggggca gaataaccac accggagtgt ggttatggta aatcccttca
    38461 tacttcaagc cacagatgca atgactcaaa ttattttggg gatacatact ttattactgg
    38521 gtactggtgg tcgtggttgt ggtggtcccg gtattagaac catcaccgcc accggttgcc
    38581 gcgagcgcga cacccaccgc cgagcttacg gtgctgacgc tggtcgcgga ggaactcccc
    38641 gccgaaaccg acgttgctgc cgactctgcc gcttcgccca cctgtacagg tgcagcccac
    38701 gcagaagtcg ccgcaagcgc agatatggca aaaatgccat acagaacttt tttcataaca
    38761 atttcccttc attgaatgaa tggagattta cccaaaaaca tttcgggcgg gatcgagtat
    38821 acacaactaa agcatgcgaa ttacggcgcg gggaattagc gatggagttt taggataatt
    38881 ggaatgaata tcaaatataa gtttcaactt atatttaaat aacaataaaa atcataagct
    38941 taaaataaaa aacaaaagta attaatctgc gtattctccg aaataaacaa cgattaaaaa
    39001 tctattttag gattaacctc acggtatgat ttccgttaaa tcacagacaa ggggatttat
    39061 ctgataaaac aatgccgact tattgtcggc attgttttta aggttattta actcctcata
    39121 ctatgcatta atgtcgatat tggcatcagg ctccggttgc cggatgcggc gtaaacgcct
    39181 tatccggcct acatatcggc atcaggcacg catgatgatt aaccgcgcca cgctttatag
    39241 cggttaatca gaccattggt cgagctatcg tggctgctga tttctttatc atctttcagc
    39301 tctggcagaa tacggtttgc cagttgttta cccagctcca cgccccactg gtcgaaggtg
    39361 aagatgttca ggatcacgcc ctgagtaaag attttgtgct catacagcgc aatcaacgca
    39421 cccaggctga acggagtgat ttcgcgcagc aggatggagt tggtcgggcg gttaccttcg
    39481 aacactttga acggcaccac gtagtcaagc gttgccggat ctttaccctg atcgcgatat
    39541 tcctgctcaa ccacttcgcg ggatttacca aacgccagcg cttcggtctg ggcgaagaag
    39601 ttagacagca gtttctggtg gtgatcagag agcgggttat gggtgatagc cggggcgatg
    39661 aaatcgcacg gtaccatttt ggttccctgg tggatcagct ggtagaacgc gtgctgaccg
    39721 ttagtgcctg gttcacccca gataatcggg ccagtctggt aatccacaac gttaccgtta
    39781 cggtcaacat acttaccgtt ggactccata ttgccctgct ggaagtacgc cgcgaaacgg
    39841 tgcatatact ggtcatacgg cagaatcgct tcagtttccg caccaaagaa attgttgtac
    39901 cagatgccaa tcagcgccag caggacaggc aggtttttct cggcaggcgt ggtggagaaa
    39961 tgcttgtcca ttgcgtgtgc gccagaaagc agttcaacga agttatcaaa gccgatggag
    40021 agaacaatcg acaggccaat cgctgaccac aaagagtaac ggccgccgac ccagtcccag
    40081 aactcgaaca tgttggcagt atcaatacca aactcgccaa cggctttggc attggttgag
    40141 agcgccgcaa aatgtttcgc aacgtgtttt tcatcacctg ccgctttcag gaaccagtca
    40201 cgcgcgctat gggcgttggt catggtttcc tgagtggtga aggttttaga tgctaccagg
    40261 aacagcgtgg tttccgggtt tacttttttc agcacttccg agatgtgagt cccatcgacg
    40321 ttagaaacaa agtgcatgtt caggtggttt ttgtacggac gcagtgcttc ggtcaccatg
    40381 tacgggccga ggtcagaacc gccgatcccg atgttcacta cgtcagtgat tgctttgccg
    40441 gtataaccct tccactcacc ggagataatc gcttccgaga aggttttcat cttctccagc
    40501 accgcgttga cttctggcat aacatctttg ccatcaacca gaatcggggt attgctacga
    40561 ttacgcagcg ctacgtgcag cacggcgcgg ttttcagtgc ggttgatctt ctcgccagag
    40621 aacatcgact taatcgcgcc cgccagatcg cactctttcg ccagatcctg taatttcgcc
    40681 agcgtctctt cagtgatgcg gtttttggag taatccacca gcatctgatc gtcgaaggtt
    40741 gcggagaact tagaaaaacg atcgccgtct ttagcaaaaa gatcggcgat cgtaacgtct
    40801 ttcatttcat cgaagtgttt ctgtagtgcc tgccaggcag cggtctgcgt tggattgatg
    40861 tttttcatta gcaatactct tctgattttg agaattgtga ctttggaaga ttgtagcgcc
    40921 agtcacagaa aaatgtgatg gttttagtgc cgttagtgta atgttgagtg taaaccctta
    40981 gcgcagtgaa gcatttatta gctgaactac tgaccgccag gagtggatga aaaatccgca
    41041 tgaccccgtc gttgacaacc gccccgctca ccctttattt ataaatgtac tacctgcgct
    41101 agcgcaggcc agaagaggcg cgttgcccaa gtaacggtgt tggaggagcc agtcctgtga
    41161 taacacctga gggggtgcat cgccgaggtg attgaacggc tggccacgtt catcatcggc
    41221 tacaggggct gaatcccctg ggttgtcacc agaagcgttc gcagtcgggc gtttcgcaag
    41281 tggtggagca cttctgggtg aaaatagtag cgaagtatcg ctctgcgccc acccgtcttc
    41341 cgctcttccc ttgtgccaag gctgaaaatg gatcccctga cacgaggtag ttatgtctga
    41401 aattgttgtc tccaaatttg gcggtaccag cgtagctgat tttgacgcca tgaaccgcag
    41461 cgctgatatt gtgctttctg atgccaacgt gcgtttagta gtcctctcgg cttctgctgg
    41521 tatcactaat ctgctggtcg ctttagcgga agggctggaa cctggcgagc gattcgaaaa
    41581 actcgacgct attcgcaaca tccagtttgc cattctggaa cgtctgcgtt acccgaacgt
    41641 tatccgtgaa gagattgaac gtctgctgga gaacattact gttctggcag aagcggcggc
    41701 gctggcaacg tctccggcgc tgacagatga gctggtcagc cacggcgagc tgatgtcgac
    41761 cctgctgttt gtcgaaatcc tgcgcgaacg cgatgttcag gcacagtggt ttgatgtacg
    41821 taaagtgatg cgtaccaacg accgatttgg tcgtgcagag ccagatgtag ccgcgctggc
    41881 ggaactggcc gcgctgcagc tgctcccacg cctcaatgaa ggcttagtga tcacccaggg
    41941 atttatcggt agcgaaaata aaggtcgtac aacgacgctt ggccgtggag gcagcgatta
    42001 tacggcagcc ttgctggcgg aggctttaca cgcatctcgt gttgatatct ggaccgacgt
    42061 cccgggcatc tacaccaccg atccacgcgt agtttccgca gcaaaacgca ttgatgaaat
    42121 cgcgtttgcc gaagcggcag agatggcaac ttttggtgca aaagtactgc atccggcaac
    42181 gttgctaccc gcagtacgca gcgatatccc ggtctttgtc ggctccagca aagacccacg
    42241 cgcaggtggt acgctggtgt gcaataaaac tgaaaatccg ccgctgttcc gcgcgctggc
    42301 gcttcgtcgc aatcagactc tgctcacttt gcacagcctg aatatgctgc attctcgcgg
    42361 cttcctcgcg gaagttttcg gcatcctcgc gcggcataat atttcggtag acttaatcac
    42421 cacgtcagaa gtgagcgtgg cattaaccct tgataccacc ggttcaacct ccactggcga
    42481 tacgttgctg acgcaatctc tgctgatgga gctttccgca ctgtgccggg tggaggtgga
    42541 agaaggtctg gcgctggtcg cgttgattgg caatgacctg tcaaaagcct gcggcgttgg
    42601 caaagaggta ttcggcgtac tggaaccgtt caacattcgc atgatttgtt acggcgcatc
    42661 cagccataac ctgtgcttcc tggtgcccgg cgaagatgcc gagcaggtgg tgcaaaaact
    42721 gcatagtaat ttgtttgagt aaatactgta tggcctggaa gctatatttc gggccgtatt
    42781 gattttcttg tcactatgag gtagcctgag tttaacggac actccttcct gaaatagaat
    42841 ggcatcagaa ggagctaata atgagcagaa aa
//
LOCUS       DAETQL010000029        42271 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.029, whole genome shotgun sequence.
ACCESSION   DAETQL010000029 DAETQL010000000
VERSION     DAETQL010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.029"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            98..1815
                     /gene="narQ"
                     /locus_tag="K0W65_001676"
                     /pseudo
     CDS             98..1815
                     /gene="narQ"
                     /locus_tag="K0W65_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            2017..5130
                     /gene="acrD"
                     /locus_tag="K0W65_001677"
     CDS             2017..5130
                     /gene="acrD"
                     /locus_tag="K0W65_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBI3094974.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(5229..5288)
                     /gene="ypfM"
                     /locus_tag="K0W65_001678"
     CDS             complement(5229..5288)
                     /gene="ypfM"
                     /locus_tag="K0W65_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBI3094975.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            5669..6025
                     /locus_tag="K0W65_001679"
     CDS             5669..6025
                     /locus_tag="K0W65_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBI3094976.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            6029..7156
                     /gene="dapE"
                     /locus_tag="K0W65_001680"
     CDS             6029..7156
                     /gene="dapE"
                     /locus_tag="K0W65_001680"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBI3094977.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMLKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            7184..7384
                     /locus_tag="K0W65_001681"
     CDS             7184..7384
                     /locus_tag="K0W65_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBI3094978.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(7494..8192)
                     /gene="ypfH"
                     /locus_tag="K0W65_001682"
     CDS             complement(7494..8192)
                     /gene="ypfH"
                     /locus_tag="K0W65_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBI3094979.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(8266..10281)
                     /gene="tmcA"
                     /locus_tag="K0W65_001683"
     CDS             complement(8266..10281)
                     /gene="tmcA"
                     /locus_tag="K0W65_001683"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HBI3094980.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(10296..11159)
                     /locus_tag="K0W65_001684"
     CDS             complement(10296..11159)
                     /locus_tag="K0W65_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBI3094981.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(11327..12040)
                     /gene="purC"
                     /locus_tag="K0W65_001685"
     CDS             complement(11327..12040)
                     /gene="purC"
                     /locus_tag="K0W65_001685"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBI3094982.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(12253..13287)
                     /gene="bamC"
                     /locus_tag="K0W65_001686"
     CDS             complement(12253..13287)
                     /gene="bamC"
                     /locus_tag="K0W65_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBI3094983.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(13304..14182)
                     /gene="dapA"
                     /locus_tag="K0W65_001687"
     CDS             complement(13304..14182)
                     /gene="dapA"
                     /locus_tag="K0W65_001687"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBI3094984.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            14328..14900
                     /locus_tag="K0W65_001688"
     CDS             14328..14900
                     /locus_tag="K0W65_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBI3094985.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14900..15370
                     /gene="bcp"
                     /locus_tag="K0W65_001689"
     CDS             14900..15370
                     /gene="bcp"
                     /locus_tag="K0W65_001689"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBI3094986.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            15623..16240
                     /gene="hyfA"
                     /locus_tag="K0W65_001690"
     CDS             15623..16240
                     /gene="hyfA"
                     /locus_tag="K0W65_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HBI3094987.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            16240..18257
                     /gene="hyfB"
                     /locus_tag="K0W65_001691"
                     /pseudo
     CDS             16240..18257
                     /gene="hyfB"
                     /locus_tag="K0W65_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            18268..19215
                     /gene="hyfC"
                     /locus_tag="K0W65_001692"
     CDS             18268..19215
                     /gene="hyfC"
                     /locus_tag="K0W65_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HBI3094988.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQNYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            19232..20671
                     /gene="hyfD"
                     /locus_tag="K0W65_001693"
     CDS             19232..20671
                     /gene="hyfD"
                     /locus_tag="K0W65_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HBI3094989.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVDFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            20683..21333
                     /gene="hyfE"
                     /locus_tag="K0W65_001694"
     CDS             20683..21333
                     /gene="hyfE"
                     /locus_tag="K0W65_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HBI3094990.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            21338..22924
                     /gene="hyfF"
                     /locus_tag="K0W65_001695"
     CDS             21338..22924
                     /gene="hyfF"
                     /locus_tag="K0W65_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HBI3094991.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            22914..24628
                     /gene="hyfG"
                     /locus_tag="K0W65_001696"
                     /pseudo
     CDS             22914..24628
                     /gene="hyfG"
                     /locus_tag="K0W65_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            24638..25183
                     /gene="hyfH"
                     /locus_tag="K0W65_001697"
     CDS             24638..25183
                     /gene="hyfH"
                     /locus_tag="K0W65_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HBI3094992.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            25180..25938
                     /gene="hyfI"
                     /locus_tag="K0W65_001698"
     CDS             25180..25938
                     /gene="hyfI"
                     /locus_tag="K0W65_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HBI3094993.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            25931..26344
                     /gene="hyfJ"
                     /locus_tag="K0W65_001699"
     CDS             25931..26344
                     /gene="hyfJ"
                     /locus_tag="K0W65_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HBI3094994.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            26374..28203
                     /gene="hyfR"
                     /locus_tag="K0W65_001700"
     CDS             26374..28203
                     /gene="hyfR"
                     /locus_tag="K0W65_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HBI3094995.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            28204..28386
                     /locus_tag="K0W65_001701"
     CDS             28204..28386
                     /locus_tag="K0W65_001701"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HBI3094996.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            28408..29256
                     /locus_tag="K0W65_001702"
                     /pseudo
     CDS             28408..29256
                     /locus_tag="K0W65_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(29294..30355)
                     /locus_tag="K0W65_001703"
     CDS             complement(29294..30355)
                     /locus_tag="K0W65_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBI3094997.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            30568..32031
                     /gene="bepA"
                     /locus_tag="K0W65_001704"
     CDS             30568..32031
                     /gene="bepA"
                     /locus_tag="K0W65_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBI3094998.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            32052..32411
                     /gene="arsC"
                     /locus_tag="K0W65_001705"
     CDS             32052..32411
                     /gene="arsC"
                     /locus_tag="K0W65_001705"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBI3094999.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(32544..33290)
                     /gene="hda"
                     /locus_tag="K0W65_001706"
     CDS             complement(32544..33290)
                     /gene="hda"
                     /locus_tag="K0W65_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBI3095000.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(33340..34629)
                     /gene="uraA"
                     /locus_tag="K0W65_001707"
     CDS             complement(33340..34629)
                     /gene="uraA"
                     /locus_tag="K0W65_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBI3095001.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(34715..35341)
                     /gene="upp"
                     /locus_tag="K0W65_001708"
     CDS             complement(34715..35341)
                     /gene="upp"
                     /locus_tag="K0W65_001708"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBI3095002.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            35665..36702
                     /gene="purM"
                     /locus_tag="K0W65_001709"
     CDS             35665..36702
                     /gene="purM"
                     /locus_tag="K0W65_001709"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBI3095003.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            36702..37340
                     /gene="purN"
                     /locus_tag="K0W65_001710"
     CDS             36702..37340
                     /gene="purN"
                     /locus_tag="K0W65_001710"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBI3095004.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            37512..39578
                     /gene="ppk1"
                     /locus_tag="K0W65_001711"
     CDS             37512..39578
                     /gene="ppk1"
                     /locus_tag="K0W65_001711"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBI3095005.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            39583..41124
                     /gene="ppx"
                     /locus_tag="K0W65_001712"
     CDS             39583..41124
                     /gene="ppx"
                     /locus_tag="K0W65_001712"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBI3095006.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(41163..>42271)
                     /gene="pdeF"
                     /locus_tag="K0W65_001713"
     CDS             complement(41163..>42271)
                     /gene="pdeF"
                     /locus_tag="K0W65_001713"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBI3095007.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFISNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         9809 a        11095 c        11019 g        10348 t
ORIGIN      
        1 ttcgctataa actgtggcag atcaaataat cccatcactg actaaattgc gtttcagcga
       61 actggaacat taatgatttt ttgtggagaa gacgcgtgtg attgttaaac gacccgtctc
      121 ggccagtctg gcccgggcct ttttttacat tgtgctgctg tcgattcttt ccacgggtat
      181 cgctctgcta actctggcga gcagtttgcg cgacgctgag gctatcaata ttgccggatc
      241 gctgcgtatg cagagttacc gcctgggcta cgacttgcaa agtggcagtc cacaactcaa
      301 tgcacatcgc cagttgtttc agcaggcact gcattcaccg gtattaacca acctcaacgt
      361 ctggtatgtg ccagaagcag taaaaactcg ctatgcgcat ctgaatgcca actggctgga
      421 gatgaataat cggctcagca agggcgattt gccgtggtat caggccaata ttaataatta
      481 tgttaatcag atagacctgt tcgtgctggc tttacagcac tacgctgaac gcaaaatgct
      541 gctggtggtg gcgatttccc tggctggcgg tatcggtatt ttcacgctgg tcttttttac
      601 tttgcgccgc atacgccatc aggtggttgc cccgctgaat cagctggtta ccgccagtca
      661 gcgtattgaa cacgggcagt tcgactcgcc gccgctggat accagtctgc cgaatgagct
      721 tggtctgctt gcaaaaacct ttaaccaaat gtcgagcgag ctgcataaat tgtacctttc
      781 gctggaggcg tcagtagaag aaaagacccg cgatctccac gagaccaagc gtcgtctgga
      841 ggtgttgtat cagtgcagtg ttcccaggcg ctgaacacca gccagattga tgtgcattgt
      901 ttccgccata ttttgcagat tgttcgcgac aatgaagcag ctgaatatct ggagctgaac
      961 gttggggata actggcggat tagcgaaggg aagccaaacc ctgagctgcc aatgcagatt
     1021 ttaccggtga caatgcaaga gacggtttac ggcgaactgc actggcaaaa tagtcacgtt
     1081 tcatcatcag aaccgctgct taacagtgtt tcatcgatgc tgggacgcgg tttgtacttt
     1141 aatcaggcgc agaagcattt tcagcagtta ttgttgatgg aagaacgtgc gaccatcgcc
     1201 cgcgaattgc acgactcgct ggctcaggtg ctttcttact tacgtatcca gttgacgtta
     1261 ctgaagcgtt cgataccgga agataacgcc accgcacaaa gtatcatggc cgatttttcc
     1321 caggcgttga atgatgctta tcggcagtta cgcgagctgt tgactacttt ccgcctgacg
     1381 ctgcagcagg cggatctccc ctccgcgttg agggaaatgc tggatacgtt acaaaatcaa
     1441 accagcgcta aactgaccct cgactgccgt ctgccaaccc tggcactgga tgcgcaaatg
     1501 caggtgcatt tgttgcaaat tattcgcgaa gcggtgctga atgcgatgaa gcacgccaac
     1561 gccagcgaaa tcgccgtcag ttgcgtcacc gcgccggacg gcaatcacac ggtttatatc
     1621 cgtgataacg ggattggtat cggtgaaccg aaagaacccg aaggtcatta tggactgaat
     1681 atcatgcgcg aacgcgcgga acggctgggc gggacgctga ctttttcaca accttccggc
     1741 ggcggcacgt tagtgagtat tagctttcgc tccgcagagg gtgaggggaa ggggaacacg
     1801 ccggctgagg tgtaaataat taccctcaca ccggccctct ccctttaaag gcgagaggga
     1861 aaaacgtgcc catattgacc tgtaagcgag tgtgtcagca atctcatgat aatttacatt
     1921 aactcctttt tttctccacg attggctcgt accttgccgc tacagtgaag caagtcaagc
     1981 ctacaacaat acgcagaaac acgaggtcct cttttaatgg cgaatttctt tattgatcgc
     2041 cccatttttg cctgggtgct gacaatcctg ttgtgtctga caggtaccct ggcgattttt
     2101 tcattgcccg ttgaacaata ccccgatctc gcgccaccga atgtgcgagt gaccgctaac
     2161 tatcccggcg catcggccca gacgctggaa aacaccgtga cccaggttat cgagcaaaat
     2221 atgaccggcc tcgataatct gatgtatatg tcatctcaga gcagtggcac cggtcaggca
     2281 tctgtcactt taagttttaa agcaggcacc gatccggacg aagccgtgca gcaagtacaa
     2341 aaccagctgc aatcagccat gcgaaagtta ccgcaggcgg tgcaaaatca gggcgtgacg
     2401 gtgcgtaaaa ccggcgatac caacattctg accattgcct tcgtctctac cgatggctcg
     2461 atggataaac aggatattgc tgattatgtt gccagtaata ttcaggaccc gttaagccgc
     2521 gtgaatggcg tcggggatat cgatgcctat ggttcgcaat attccatgcg tatctggctg
     2581 gacccggcga aactcaacag tttccagatg acggctaaag atgtcactga tgccattgag
     2641 tcacagaacg cgcagattgc ggttgggcaa cttggtggta caccttccgt cgataagcag
     2701 gcgctcaacg ccaccattaa cgcccagtca ctgctgcaaa caccagaaca gttccgcgat
     2761 atcaccttgc gggtgaatca ggacggctca gaggtaaggc tgggcgatgt cgccaccgtc
     2821 gaaatggggg cggagaaata cgattatctt agccgcttca atggtaagcc agcctccggg
     2881 ttgggggtaa aactggcctc cagcgcaaac gaaatggcga cagcggagct ggtgctcaat
     2941 cgtctcgacg agctggcgca gtatttcccg cacggactgg aatacaaggt ggcgtatgaa
     3001 accacctcgt ttgttaaagc ctccattgaa gacgtggtga aaacgctgct ggaagctatc
     3061 gctctggttt tcctcgttat gtatctgttc ctgcaaaact tccgcgccac gctgataccc
     3121 actatcgccg tgccggtggt gttgatggga accttctccg tactttacgc cttcggttac
     3181 agcgtcaaca ccttaaccat gttcgcgatg gtgctggcga tcggtctgct ggtggatgac
     3241 gccatcgtgg tggtggaaaa cgtcgaacgt attatgagtg aggaaggact cactcctcgc
     3301 gaagccacac gtaaatcgat ggggcagatc cagggggcac tggtcgggat tgcgatggtg
     3361 ctttcggcgg tatttgtacc aatggccttc ttcggcggca ccaccggtgc catctatcgc
     3421 cagttctcta ttaccattgt tgcggcgatg gtgctgtcag tactggtagc gatgctcctc
     3481 actccggctc tgtgtgccac gctacttaag ccactgaaaa aaggtgagca tcacgggcaa
     3541 aaaggttttt ttgcctggtt taaccagatg tttaaccgca acgccgaacg ctacgaaaaa
     3601 ggggtggcga aaattctcca ccgtagcctg cgctggattg tgatttatgt cctgctgctt
     3661 ggcggcatgg tgttcctgtt cctgcgtttg ccgacgtcgt tcttaccgct ggaagaccgg
     3721 ggcatgttta ctacctcggt acaattgccc agcggttcaa cccaacaaca gaccctgaaa
     3781 gtcgttgagc aaatcgagaa atattacttc acccatgaaa aagacaacat catgtcggtg
     3841 tttgccaccg ttggttctgg ccctgggggt aacgggcaaa acgtggcgag aatgtttatc
     3901 cgcctgaaag actggagcga acgcgacagt aagaccggca cctcgtttgc cattatcgag
     3961 cgtgcaacga aggcgtttaa ccaaattaaa gaagctcgcg ttatcgccag cagcccacca
     4021 gcaattagcg gtcttggtag ttctgcaggt tttgatatgg agttgcagga ccacgctgga
     4081 gcgggtcacg atgcgctgat ggcagcacgt aatcagttgc tggcgctggc ggcggaaaac
     4141 ccggagctaa cccgtgtgcg ccataacggc ctcgatgaca gtccgcagtt gcagattgat
     4201 atcgaccagc gtaaagctca ggcgctgggc gttgatatcg acgatattaa cgacacactg
     4261 caaaccgcct ggggttcgag ctatgtgaat gactttatgg atcgcggtcg cgtgaagaaa
     4321 gtctatgtgc aggcagctgc gccgtatcgc atgctgccag atgacatcaa tctctggtat
     4381 gtccgaaata aagatggcgg catggtgccc ttctctgctt tcgcgacctc acgctgggaa
     4441 acaggctcgc cgcgtctgga acgctataac ggttattctg cggttgagat tgttggggaa
     4501 gccgcaccgg gggtcagtac cggtacggcg atggatatta tggaatcgtt agtgaagcag
     4561 ctgccaaacg gctttggtct ggagtggacg gcgatgtcgt atcaggagcg gctttccggc
     4621 gcgcaggctc cggcgctgta cgccatttcc ttgctggtgg tattcctgtg tctggcggca
     4681 ttgtatgaaa gctggtcggt accgttctcg gtaatgctgg tcgtgccgct gggggtaatc
     4741 ggcgcgctgc tggcaacctg gatgcgcggg ctggaaaacg acgtttactt ccaggtgggc
     4801 ctgttaacgg tcattggttt atcggcgaaa aacgccatcc tgatcgtcga gtttgctaac
     4861 gagatgaacc aaaaaggcca tgacctgttt gaagcgacgc tccacgcctg ccgtcagcgt
     4921 ttacgcccga ttctgatgac ctcgctggca tttatcttcg gcgtattgcc aatggcaacc
     4981 agcacgggtg ccggttccgg tggtcagcat gcggtgggta ctggcgtaat gggcgggatg
     5041 atttcagcta ctattctggc tatttacttc gtgccgctgt tctttgtgct ggtgcgccgc
     5101 cgcttcccgc tgaagccgcg cccggaataa gcaataaaaa aggcgacatg ccaatgtgtc
     5161 gcctttttca actttccgat taagaacctg ctcagcgggt tcttgctgtt tatactttgt
     5221 ctcaggaatt acttacgaag cataacttcg ataaagtctt tccagttccc cagttcacgt
     5281 tcaatcataa caacctctct tataattatg ggtattctac ggaaacaata taccgtggtg
     5341 aagctaattt actcgattgc tgcgatgact acctccgggg gacaaacctt atgtaaatac
     5401 tatggtcctg cggtgataat ttgtatgtga tacacagcaa catttcgaga tattcatacg
     5461 gcatctaata ctgatttaat tctggttaaa atacagacag ataacaagat gaatattctt
     5521 aatgtttacg ttaaaaatgt ttaatattat ttaatagttg ttaatttgaa tacttcgata
     5581 atgttatatt ttctgataat catttgcagg caaaatgttt tcacccttaa atgagtattt
     5641 attctcataa atcgaaaaag gattcattat ggttacactt tacggcatca aaaattgtga
     5701 caccattaaa aaggctcgcc gttggctgga agccaataac atcgactatc gttttcatga
     5761 ttaccgcgtc gatgggctgg acagcgaatt attgaacggt tttatcaacg aattaggctg
     5821 ggaagcgtta ctcaacaccc gtggtacaac ctggcgtaaa ctggacgaaa ctacccgcaa
     5881 taaaatcacc gatgcggcct ctgcggcggc attaatgact gaaatgcctg caattatcaa
     5941 acgtccattg ctctgcgcgc ctggtaagcc tatgctgctg ggtttcagtg attccagtta
     6001 tcagcaattt ttccatgagg tgtagtctat gtcgtgcccg gttattgagc tgacacaaca
     6061 gcttattcgc cgcccttccc tgagtcctga tgacgcagga tgtcaggctt tgttgattga
     6121 acgtttgcag gcgatcggtt ttaccgttga acgcatggat tttgccgata cgcagaattt
     6181 ttgggcatgg cgtgggcaag gtgaaacgtt agcctttgcc gggcataccg acgtggtgcc
     6241 gcctggcgac gccgatcgtt ggatcaatcc gccgtttgaa cccaccattc gtgacggcat
     6301 gttattcggg cgcggtgcgg cagatatgaa aggctcgctg gcggcgatgg tggtagcagc
     6361 agaacgtttt gtcgcacaac atcccaacca tacggggcga ctggcatttc tgatcacttc
     6421 tgatgaagaa gccagtgccc acaacggtac ggtaaaagtc gtcgaggcgt taatggcacg
     6481 taatgagcgt ctcgattact gcctggtcgg cgaaccgtcg agtatcgaag tggtaggtga
     6541 tgtggtgaaa aatggtcgtc gcggatcgtt aacctgcaac ctcaccattc atggcgttca
     6601 ggggcatgtt gcctacccac atctggctga caatccggta catcgcgcag cacctttcct
     6661 taatgaatta gtggctattg agtgggatca gggcaatgaa ttcttcccgg cgaccagtat
     6721 gcagattgcc aatattcagg cgggaacggg cagtaacaat gttattccgg gtgaactgtt
     6781 tgtgcagttt aacttccgct tcagcaccga actgactgat gagatgctca aagcgcaggt
     6841 acttgccctg cttgaaaaac atcaactgcg ctatacggtg gattggtggc tttccgggca
     6901 gccatttttg accgcgcgcg gtaaactggt ggatgcggtc gttaacgcgg ttgagcacta
     6961 taatgaaatt aaaccgcagc tactgaccac aggcggaacg tccgacgggc gctttattgc
     7021 tcgcatgggg gcgcaggtgg tggaactcgg gccggtcaat gccactattc ataaaattaa
     7081 tgaatgtgtg aacgctgccg acctgcagct acttgcccgt atgtatcaac gtatcatgga
     7141 acagctcgtc gcgtgatgag tggttctgca agaggaaata agcatggact ggctggctaa
     7201 atattggtgg attctggtga ttgtcttttt ggtaggcgtc ctgctgaacg tgattaaaga
     7261 tctcaagcgc gtcgaccata agaaatttct cgccaacaag ccggagcttc ccccgcatcg
     7321 tgatttcaac gataagtggg acgatgacga cgactggccg aaaaaggatc aaccgaagaa
     7381 gtaagctgag tgctcaaccg taggccggat aaggcgttaa cgccgcatcc ggcaatggtg
     7441 aacgatccct gatgcgacgc tcacgcgtct tatcaggcca ggtaatctgt cttttacatc
     7501 atttcaatca catcatcatc gccaggctta ccgccactta gcgcttggtc gaaataatgt
     7561 ttcggaatgg tataacgcaa gtgatccagg gcaaattgca tactgcgatt gtcgatcgca
     7621 tggcctaaat cctccacgat atccagcgtc acgtcaccac cggcactgat taacgcttcc
     7681 tgcgccgcaa ctgcatgcgc cagatcgata accggatctt caccaccatg aatcaagtga
     7741 attgtcgtgg ctgtagacgc ggtctccggc aggctcgcat agcgtccgtt aaaggcaatg
     7801 acgcgcgaag caaggcccgg ttcggcttta atgctctcca gcgccataat cgcgccttgt
     7861 gagaaaccaa ttagcgccgt ggcgttagcc cctactccgc tctgtttctg ccagtagcgt
     7921 accgtttcaa taaacgtcgg catgatggca tccacacgcg cctggcgatt atcttccgta
     7981 ataccctgca ctgaaaacca ctgacgcccc gccggattac cgctcggctc cgcgccaccg
     8041 acactcacca ccagcgcatc aggaaacagc ggtgcaaacc agctgcctat ttcccccatt
     8101 gccaccgggt tatccccgac accgtggaaa agcagtaaca actgttgtgc aggtttatcc
     8161 gggctttgaa caacaaaatg gtcatgtttc atggcgaact ccttaactga tgtcattaat
     8221 tttacgccgc tgactcacaa tgaccatggg agaaaattga aggagtcagt gaaaaaattg
     8281 ccattgcgtt atgcgatcgc gcagacgctc agtgcgaacc tcatttagtg cgaacagcgc
     8341 ctgcgcggcc tcttcccgct ggcgaaccag taacatcttg cggcctgaaa gtttaagtgt
     8401 ggtacataac tgcgcgtcac tggcgttttt ctgtaaacgc ccacgcagcg ccggtaatgc
     8461 cagttcactg gtttgtagca gacgcaataa gcaacctaac gatgttaata gcggacgatg
     8521 agcgaaagca aaaccggcca gttcaagcca gtcgtcgtca gaaaggatgg catcgtttag
     8581 tggatcaaca ggaagcattt cgccattcca ctgcgcgaga gcttgcgcat cgcgacgtaa
     8641 acggtaatgc tcacgttcag ccagctgttt acccgcatca ctcatcggta acagcgccat
     8701 cgccgtatag caaccgctgc tggcttcccg atgattaccc atccgcacca gcacaaaacc
     8761 gcagcgttgc cagaaacgcc ataactcccc agtgtaacca aaactcaccg aaagatagtc
     8821 gaggtcatgc gtatattgca aagctccagc aataagttgt cgccctgtgc cttcccgctg
     8881 acgagccgga tgaactgcta tccggctaac ccgccttccg cgcaatgtcg ccgccagtgg
     8941 attgctgccg tgcgccgcca gcgactgggc caccagatta ccccgcgggc gacgaaaacc
     9001 tgcccatacc gcctgactga gttcttgaga taatccgccc tcatccacca gccacaacgc
     9061 cccggcaatc tcgttttcgc cagccgcctg taaaaaatgt tgccctggtg catccatcat
     9121 ccggcgtaaa tccagcggcg aggtccggta gtgcgcacca gacaataact gataaacctt
     9181 taacggcgtt tctggctcgc ttcgccataa cgtttgttca aatgcggaaa tgacgatatt
     9241 gccttgtggt gtatgggtga agttttcatc gttaaaaacc agtgcctcac taaccatttt
     9301 ttccagcggg catccctgtg cccagcggat cggctgttgc agttcaaaac ggtgtaaatg
     9361 cggaaagcga gcgcaaaatt tcagcaaaaa accacgtccg gtaccttcgt agccctgcac
     9421 cgtagtggtt aacaacgttc gaggaaaacg cgataccagt tgatgcaaca acggcgcagg
     9481 tatggctgcg gcttcgtcga ccaccagcca gtcggcttgc tcatcgctgg ctaacaaggc
     9541 atccggtgca ataaagcgaa acttctcgcc cgcaaattgt gccagtacat ccgttgccgc
     9601 ttttgcgggc gcggtgacaa tcgcactgcc cgcaatacga gaaatgagtt gccctgccaa
     9661 cgccgactta ccgcgcccac gcgcagccgt taccgctgcc acgcccggcg gcatggtcag
     9721 tagctgctgt aagagttgct gttgttctgg ttgtggtgcg ccggtcgcgg ggtgccagtc
     9781 agtacgggga gtaaaatgcg ccaacgagaa cggctggttt tgccgccaga ggatagcgtc
     9841 gttatccgcc gtaagtacgc gtttgagatg ctggacaaaa tgcggcgtcg caatagggtc
     9901 agggcaatca ctccagcgca gcgagtcggc atcaggttgg ttttcccact cttcccatac
     9961 agggagtaac aaaaccagcc agcttcccgc tttcaacgtt ccgctaagtg ctgcaaaggc
    10021 agcggcatca aagccgtggc gggcgtcgaa taccgcatgc cggaactcgc gcccaagtaa
    10081 agtttgtagc gccgagggtg aacagtggtt ttcagcatct agctgcggcg aaatccacag
    10141 ccagtcgcca ggtaaggcat cacgcaactt aagagcatga tcaaaacacc aaccctcttc
    10201 cccgctcaac accagcaagc ggcagatccc ttcacgtttc atttgcgctg ttaatgtgta
    10261 aagcgcagtc agttcagcca tccctgcccc ggaaattaaa tgcttttacc aaaagtattg
    10321 cattgtgccg gatcgccgct gtcgaaacca cgtttaaacc agctgtagcg ttgctgagaa
    10381 gtgccatgtg tgaaactgtc tggtactact cgcccctgac tttgctgttg taaacggtca
    10441 tcgccgatgg cctgcgccgc attcagcgcc tcttccagat cgccagtttc cagaacgcct
    10501 tgctgctgca tactatgccc ccagacaccg gcaaaacagt cggcctggag ttccatacgc
    10561 acagataagc ggtttacttc cgcctgcgtc gcgttttgtt gcagttgacg aactttcggc
    10621 tcgatgccta acagtttctg cacatgatga ccgacttcat gggcgataac gtacccctgg
    10681 gcaaaatcgc catccgcgcc aagtttgtct ttcatgtcat catagaagga gagatcgata
    10741 taaactgtgc catccgccgg gcaatagaac ggccccataa tggactggcc cgccccgcag
    10801 ccggtgcgcg tcattccacg gtacatgacc agcttcggtt gctgataggt cttacccatc
    10861 ttttcgaact gttgtcccca ggtgtcttcc gtggttgcct gaatcaccga ggtgaatttt
    10921 gcggcttcgt cttcattagg gctaattgac cgcgttgatt gttgttgaga aaccggctgc
    10981 ccggtcatca acccggttaa atcaacgcca tagtagcctg caaccagcac gactatcagt
    11041 aaaatcagcc cgcctttacc gctcggcaga cggaaaccgg gaccgcccat tgacggaccg
    11101 ccagagctgt tgcgcctgtc ttcaacattg tcactttcac gtcgcccttg ccaacgcata
    11161 gatacctcaa caatatattc attatagaaa tgatcgtagg tggttcagag gaagattacc
    11221 acaggaaagc gggatgaaat ggcggaggaa tgagcgttca atgcatatat tccgagagcg
    11281 gttgtcttgc cgtgccagct gcacggcaag atgatgaaca gaaaaatcag tccagctgta
    11341 cacccaggcg gcgggcgacg gcttcatagg cttcgatcag gccaccgagg ctctggcgga
    11401 aacggtcttt gtccattttc tccagtgttt ctttgtccca caggcggctg ccgtccgggg
    11461 agaactcatc gcccagtacc acttcacctt tgtacagacc aaactccagc ttgaagtcga
    11521 ccagaatcag accagcatca tcgaacagtt ttttcagcac gtcgttcgct ttgtaggtca
    11581 gctctttcat acgcgccagg ttctctttgc tcacccagcc aaaggtttcg cagtaagatt
    11641 cgttgaccat cggatcgtgc atggcatcgt ttttcaggaa cagatcgaac agcggcgggt
    11701 tcagctcaat accttcttcg attccaagac gtttcactag agagccagca gcacggttac
    11761 gcacgacaca ctcaaccggc accatatcca gctttttcac cagacattcg gtatcggaga
    11821 gcagacgctc catttgagtc gggatacccg cttcagccag tttgctcata atgaagtagt
    11881 tgaacttgtt gttcaccata cctttgcgat caaactgctc aatgcgcgcg ccatcccctg
    11941 ctgacgtatc attgcggaat tcgagcacca acaggtccgg gttttccgtg ctgtatacgg
    12001 ttttcgcttt accacgatac aactcagctt gcttttgcat ctttatcact cctgggtgtg
    12061 aattaacgtt ttaaaatctt ttgctgtctg gtgtgccgga tgttttgtcg gatgcggcgt
    12121 gaacgcctta tccgaactac gtccgacaaa caaatttcgt gcgaattacc gctaaaatcg
    12181 cgttttcctg ccgacgcaca cgtttgcgta tcatatcaga aaaaagggcc ggatgattcc
    12241 agccctgtat ttttacttgc taaacgcagc ctggaagaca gctaccagcg cgtcgttctg
    12301 actctgagtc agagtatgac ctttcggatc gatgaactgt aggctgctgc ggttatctaa
    12361 atcgccaacc tgcagtttat agtcaccgga tgccaggcct ggatcgctcg cgcccagttc
    12421 ctgccagtcg ctgtcagaca gcggcttata agttacggcc atgttgccct gcgaacgggt
    12481 gctgtcggtc actttcatgc ccactttttc cagcgccgct ggcagacgtt gccaaaccac
    12541 attgaacggc ccgcgtacga ccagcattgg taaaccggtg tcatcagctg cactttgtac
    12601 gtccatagtg gtggaggcac gattttgcgc agcgttcgcg gcgtcagtgg cagatttatc
    12661 cagaccggcg gaaataacgt tcatcatctc cgtgctgtaa cgctgcatgg aagccgcgtc
    12721 tgcaaccggt ttgcccgcct gttccaggtt cagcagttta accgtaaccg cctgctgata
    12781 accctgcggc ttaacagaga tttgataacg accacgatac tgctcgtctt cgtccagacg
    12841 gttccattgt acccaatcgg tggtcagtgt ctgaccagca tcatcacgtt gggtgatggt
    12901 gtagtttttc gcctgcagca cgctaaccac ctgcggccac agagtattgc cacgaccatt
    12961 ttccaccagc aatgaagcgg tatcgcccgt gaactgggta cgcgcgccag aaaccagtgc
    13021 caacggctgg gctggtggac gaatgtccag cgccttaccg acagcaccac taccgttggt
    13081 caccgggatt gcataatcac cggaggtcac cggcaaaatc attccagccg gggcatgaag
    13141 ctccgcaagc ggtgccgctt ccaggtaggc ttcatcacca ctgacctgac gcttatagcg
    13201 tgagtcagaa ctacaggcag cgagtaataa aacaagcgaa acacccgcaa cctttgccag
    13261 gcgcgacttt tgaacagagt aagccatcaa atctccctaa actttacagc aaaccggcat
    13321 gcttaagcgc cgctctgacc gtctcacgac cactgtcggt gattggtgtc attggcaggc
    13381 gcagcgtatc ggtcgccaca agacccagtt ccttacatgc ccatttcacc gggattggat
    13441 tgggttcgac aaatagtttg ttgtgtaatg gcatcagacg ctgattaata acgcgtgcct
    13501 cggcaaaatg cccttctgct gccagtttgc acatctgggc catatcacgc gctgcgacgt
    13561 tagccgtaac ggaaataact ccatgaccgc ccaattgcat gaagtccagc gcgctcgcat
    13621 catcgccgct cagcagaaca aaatcatctg aaaccagctc tttgatctgg tttacacgcg
    13681 ttaagttccc tgttgcctct ttgattccga taatattttt tactttcgcc agacggccca
    13741 ccgtttccgg gagcagatcg cagccagtac gggacggcac attatacaga atttgcggca
    13801 ggtcagtatg ctcagcgatg gctttgaaat gctgatacaa accttcttgc gacggacgat
    13861 tgtagtaagg ggttaccgtc aggcagccga cgataccact gtcattgaag cgctgcgtca
    13921 ggctaatggc ttccgcagta gcgttagcgc cggtcccggc aattaccgga atgcgcccat
    13981 cagccagatc cagcgtcatc atcaccacat cagcatgttc gtcatgattt aaggtagcgg
    14041 actcgccagt ggtgccaaca gaaacgatcg ccgaagtacc gctggcgaca tgataatcaa
    14101 tcagtttttt caagctagcc cgacagacat tacctttttc atccatcgga gtaacaatcg
    14161 cgacaatact tcccgtgaac atgggccatc ctctgtgcaa acaagtgtct caatggtacg
    14221 tttggtatgg cattaaaagc aagcagacag aaccgttctg attgttgtat gcatgatttt
    14281 tttatgcttt ccttaagaac aactcacccc ttaaaggaat aaccagtttg acactgtcat
    14341 cgcaacatta tctggtgatc actgcgttgg gtgccgatcg ccctggaatt gtgaacacca
    14401 tcacccgtca tgtcagtagt tgcggctgta atattgaaga cagtcgcctg gcgatgctgg
    14461 gagaagagtt cacgtttatc atgctgcttt ccggttcatg gaatgccatc actctgattg
    14521 aatcaacgtt accgttgaaa ggtgccgaac tggatctttt aatcgtgatg aagcgcacga
    14581 cggcgcgtcc gcgtccgcca atgccagcat ctgtctgggt tcaggtcgat gttgcagact
    14641 ccccgcattt aattgaacgc ttcacagcac ttttcgacgc gcatcatatg aacattgcgg
    14701 agctggtgtc gcgcacacaa cctgctgaaa atgaacgggc tgcgcagttg catattcaga
    14761 taaccgccca cagccccgca tctgcggacg cagcaaatat tgagcaagcg ttcaaagccc
    14821 tatgtacaga actcaatgca caaggcagta ttaacgtcgt caattattcc caacatgatg
    14881 aacaggatgg agttaagtaa tgaatccact gaaagccggt gatatcgcac cgaaatttag
    14941 cttgccggat caagacggag aacaagttaa tttgaccgac ttccagggac agcgtgttct
    15001 ggtttatttc tacccgaaag ccatgacccc cggctgtacc gtacaggcct gcggcttacg
    15061 cgataacatg gatgagttga aaaaagcggg cgttgatgtg ctgggtatca gcaccgataa
    15121 acccgaaaaa ctctcccgtt ttgcggaaaa agagctgctt aactttacgc tcctgtctga
    15181 tgaggaccac caggtgtgcg aacaattcgg cgtctggggt gaaaagtcct tcatgggcaa
    15241 aacctacgat ggtattcatc gcatcagctt cctgattgac gctgatggca aaatcgaaca
    15301 tgtctttgac gatttcaaaa ccagcaatca ccacgacgtt gtgctgaact ggctgaaaga
    15361 acacgcctga ttactttgct ccattccgtg ctggctgcgc ttgcggccag catacctcac
    15421 ttctcgtgat caagatcaca ttctcgcttt cccctgcgac acgggtgtcg aatccatttt
    15481 ttgctgaacg ttaatgacca tcatttttgt accgttcaga atccagttaa tacataactt
    15541 attgaataga ttgagttaat cagaatggca tcctttatac aatatgaaat gcaatgtttc
    15601 atatcatttt caaggagccg acatgaaccg ctttgtggtg gccgaaccac tgtggtgtac
    15661 aggatgtaat acctgtctcg ctgcctgttc ggacgtgcat aaaacgcaag gtttacagca
    15721 acacccgcgc ctggccctgg cgaagacgtc aacaatcact gcccctgtcg tgtgtcatca
    15781 ctgtgaggaa gccccttgcc tgcaggtctg cccggtcaat gccatctctc agagggatga
    15841 tgcgatccaa ctcaacgaaa gcctctgtat tggctgcaag ctttgcgccg tggtctgccc
    15901 atttggcgca atcagcgctt caggaagccg tccggtgaat gcccatgcgc aatatgtttt
    15961 tcaggctgaa ggctcactca aagacggcga agaaaacgtg ccgccccaac atgcgttgct
    16021 gcgctgggaa ccgggtgtcc agaccgtcgc ggtgaaatgc gacctgtgtg atttcttgcc
    16081 agaaggtccg gcctgcgttc gcgcttgccc gaatcaggcg ttacggctga tcaccgacga
    16141 tagcctgcaa cgtcagatga aagaaaaaca gcgccttgcc gcaagctggt ttgccaatgg
    16201 cggggagtct cccctttccc tcactcagga gcaacactaa tggatgccct gcaattatta
    16261 acctggtcgc tgatcctcta tctgtttgcc agtctggctt cgctgttttt actcggtctg
    16321 gacagactgg ctattaagct ttccggcatc acatcgctgg tgggcggcgt gattggcatc
    16381 atcagcggaa ttacgcaatt acatgctggt gtaactttag tcgcccattt tgccacccct
    16441 tttgactttg ccgatttaac cctgcgaatg gatagcctct cggcatttat ggtgctagtt
    16501 atctccttgc tggtggtggt ttgttcgctc tattcattga cttatatgcg cgaatacgag
    16561 ggcaaaggcg cggcggcgat gggcttcttt atgaatcttt tcatcgcatc gatggttgcc
    16621 ctgctggtga tggacaacgc tttttggttc atcgtgctgt ttgaaatgat gtcgctgtct
    16681 tcctggtttc tggtcattgc caggcaggat aaaacgtcga tcaacgctgg catgctctac
    16741 ttttttatcg cccacgccgg atcggtgctg attatgatcg ccttcttgct gatggggcgc
    16801 gaaagcggca gcctcgattt tgccagtttc gcacgctttc actttctccg gggctggcgt
    16861 cggcggtgtt tctgctggcc tttttcggtt ttggcgcgaa agccgggatg atgccgttgc
    16921 acagctggtt gccgcgcgct caccctgccg caccatcgca cgcttcggcg ttgatgtctg
    16981 gcgtaatggt caaaatcggt attttcggca tcctgaaagt agcgatggat ctgctggcgc
    17041 aaacgggttt gcctctgtgg tggggcattc tggtgatggc gatcggcgca atctccgcgc
    17101 tcctgggcgt gctatatgcg ctggcggaac aggatatcaa acggctgctg gcctggagta
    17161 ccgtcgaaaa cgtcggcatt attttgctgg cagtcggtgt ggcgatggac ggtctgtcac
    17221 tgcacgaccc gctgctcacc gtggttggac tgctcggcgc actgtttcat ctgctcaacc
    17281 atgcgctgtt caaagggctg ctatttctcg gcgcggacgc gattatttcg cgtttgcata
    17341 cccacgacat ggaaaaaatg ggggcactgg cgaaacggat gccgtggaca gccgcagcat
    17401 gcctgattgg ttgcctcgcg atatcagcca ttcctccgct gaatggtttt atcagcgaat
    17461 ggtacacctg gcagtcgctg ttctcactaa gtcgtgtgga agccgtagcg ctacaacttg
    17521 cgggtcctat tgctatggta atgctggcag tcactggtgg gctggcagta atgtgcttcg
    17581 tcaaaatgta cggtattact ttctgtggtg cgccgcgcag tacacacgct gaagaggcac
    17641 aggaagtgcc aaatacgatg atcgtcgcca tgctactgct cgcggcactc tgcgtattaa
    17701 ttgcgcttag tgccagttgg ctggcaccga agataatgca tattgcccat gcgtttacca
    17761 atacccctcc cgtcactgtc gccagcggaa tagcacttgt acccggcacg tttcatacac
    17821 gggtcactcc ttcattactg ttgctgttac tactggcgat gcctttgctg ccaggccttt
    17881 actggctgtg gtgtcgttcg cgccgcgcag cgtttcgtcg cacaggagat gcctgggcat
    17941 gcggctacgg ctgggaaaac gcgatggctc cgtcaggcaa tggcgtgatg cagccgctgc
    18001 gtgtggtctt ctctgcgtta tttcgtctac gacaacagct cgaccctacg ctgaggctaa
    18061 ataaaggtct tgcgcacgtc accgccaggg ctcagagcac agaacccttc tgggatgagc
    18121 gggtgatccg ccccatcgtg agcgccaccc aacggctggt caaagaaata cagcatctgc
    18181 aaagcggcga ctttcgtctc tattgcctgt atgtggtcgc cgcactggtt gtgctgctaa
    18241 tcgctattgc cgtctaagga aatcaccatg agacaaactc tttgcgacgg atatctggtc
    18301 atttttgcgt tagcacaggc cgtgattctg ctgatgctaa ccccactttt tacgggtatt
    18361 tcccggcaga tacgcgcgcg tatgcactcc cgccgcgggc cggggatctg gcagaattat
    18421 cgcgatatcc acaaactgtt taaacgccag gaagttgcgc cgacatcttc aggtctgatg
    18481 ttccgcctga tgccgtgggt attaatcagc agcatgctgg tgctggcgat ggccttacca
    18541 ctatttatta ccgtttcccc ttttgcgggc ggcggcgatc tgatcaccct tatctatctt
    18601 cttgccctgt ttcgtttttt ctttgctctt tccgggctgg ataccggaag tccgtttgcg
    18661 ggagtcggtg ccagtcgcga gttgacgctc ggcattctgg tcgaaccaat gcttattctc
    18721 tcactgctgg tattggcgct gatagcaggt tccacgcata tcgagatgat cagcaatacg
    18781 ctggctatgg gctggaactc gccgctaacc accgtactgg cgttactggc ctgtggtttt
    18841 gcctgcttca ttgagatggg aaaaattccc tttgatgttg ctgaagcaga acaggaatta
    18901 caggaaggcc cgctgaccga atattccggt gccgggctgg cgctagcgaa atgggggctg
    18961 gggctgaaac aggtcgtgat ggcagcactg tttgtggccc tgtttctgcc ctttgggcgc
    19021 gcgcaagaac tttctctcgc ctgcctgctg acttcacttg tcgttacgct gctcaaggtt
    19081 ttgctgattt ttgtactggc ctcaatcgca gaaaacacgc tggcacgcgg gcgtttttta
    19141 ctcattcacc atgtgacctg gcttggcttc agccttgctg cgcttgcatg ggtcttttgg
    19201 ttaaccggtc tgtaaggagc actgacggaa tatggaaaat cttgctctga cgacgttaat
    19261 gctgcctttt atcggcgcac tggtcgtttc gttttcgcca caacgtcggg ccgccgaatg
    19321 gggggttttg ttcgccgcgc tgaccacgct gtgcatgttg tcgctgatct ccgcgtttta
    19381 tcaggccgat aaagttgccg tcacgttgac gttggtcaac gtgggggatg tggcattatt
    19441 tggcctggtc attgatcgcg tgagtacgct gattctgttt gtggtggtgt ttctcggttt
    19501 gctggtcacg atctactcca cgggttatct gacggataaa aatcgcgaac acccgcataa
    19561 cggcacgaat cgttattacg catttttgct ggtgtttatc ggcgcgatgg cgggactggt
    19621 actctcctcg acgctgctcg gtcagttgtt gttttttgaa attacgggcg gctgctcctg
    19681 ggcgttgatc agttattacc agagcgataa agcgcagcgt tcagcactaa aagcgttgct
    19741 tatcacccat atcggttcgt tggggttgta tcttgccgcc gccacgctgt ttttgcagac
    19801 cggaacgttt gtgcttagcg cgatgagcga gttacatggc gacgcacgtt atctggttta
    19861 tggcggcatc ctgtttgccg cgtgggggaa atcggcccag ctaccgatgc aagcgtggct
    19921 accggacgca atggaagcgc caacaccgat cagcgcctat ctccacgccg catcgatggt
    19981 gaaagtgggc gtttacattt ttgcccgcgc cattatcgac ggcggcaata tcccgcatgt
    20041 gattggcggc gttggcatgg tcatgacact ggtcaccatt ctttatggct ttctgatgta
    20101 tttgccacag caggatatga agcggttgct ggcctggtcg accatcactc aacttggctg
    20161 gatgttcttc ggcttgtcgc tctccatctt cggctcgcgg ctggcgctgg agggtagcat
    20221 cgcctacatc gtcaaccacg cgttcgctaa aagcctgttt ttccttgtag caggtgcgct
    20281 gagttacagc tgcggcacgc gcttgttgcc gcgtctgcgt ggcgtattgc acaccctgcc
    20341 gttgccaggc gtggatttct gcgtggcagc gctggcaatt accggtgtgc cgccgttcaa
    20401 cggcttcttc agtaaattcc cgctgtttgc cgccggtttt gcgttgtcag tggagtactg
    20461 gatcctgctg cccgccatga ttctgctgat gattgaatcg gtcgccagtt tcgcctggtt
    20521 tattcgctgg tttggtcgcg tcgtgccggg caaaccgagc gaggccgtcg ccgatgccgc
    20581 accgctgcca gggtcaatgc gcctggtgtt gattgtactg attgtgatgt cgctgatttc
    20641 cagcgtaatc gccgcgacct ggttgcagta aggagatgat gaatgaccgg ttctatgatc
    20701 gtaaataatc tggcgggact gatgatgctg acatcgctgt ttgtgattag cgtcaaaagc
    20761 tatcgcctgt catgcggatt ttacgcctgc cagtcgctgg tgctggtgtc tattttcgcc
    20821 actctctcgt gcctgttcgc cgcagagcaa ctgctgatct ggtccgccag cgcctttatc
    20881 accaaagtgc tgctggtacc gttaatcatg acttacgctg cacgaaatat tccccagaac
    20941 atcccggaaa aagcgttatt cggtccggca atgatggcac tgctcgcggc gttaattgtc
    21001 ctgctttgcg catttgtcgt tcagcccgtg aagctaccga tggctaccgg gctgaaaccg
    21061 gcgctggcgg tagcgttagg tcattttctg cttggcctgc tgtgcattgt cagccaacgc
    21121 aatatcctgc ggcaaatttt tggttactgc ctgatggaaa acggctccca tctggtgctg
    21181 gcgcttcttg cctggcgagc accggaactg gtggaaatag gtatcgctac cgacgccatc
    21241 ttcgccgtca ttgtgatggt gttactggca agaaaaatat ggcgtaccca cggcacgctg
    21301 gacgtgaaca acttgaccgc gctgaaggga taatgagatg agttattctg tgatgttcgc
    21361 tttactcctg ctcacgccgc tgcttttttc gctgctctgt tttgcctgcc ggaaacgggg
    21421 gctctctgcg acttgtacgg tgactgtatt acatagctta gggatcacac tgctgctgat
    21481 tctagcactc tgggtggtcc aaactgccgc tgatgcagga gaaatattcg ctgcgggact
    21541 gtggcttcat attgatggtc tgggcggttt gttcctcgcc attcttggtg tgattggctt
    21601 tctcaccggt gtttactcga ttggctacat gcgtcatgaa gtggcacacg gcgagctttc
    21661 acccgttaca ctgtgcgatt actacggttt cttccatctg tttttgttca ccatgctgct
    21721 ggttgttacc agcaataacc tgattgtgat gtgggcggcg atcgaagcca ccaccttaag
    21781 ctcggcgttt ctggtaggca tttacggtca gcgttcatcg ctggaatcag catggaagta
    21841 catcattatt tgtactgttg gtgtcgcttt tggtctgttc ggtaccgtgc tggtatacgc
    21901 caacgccgcc agcgttatgc cgcaggcaga aatggcgata ttctggagcg aggttcttaa
    21961 gcaatcgtcc ttgcttgacc caacattaat gctgttggcc tttgtgtttg tgctaattgg
    22021 ctttggtacc aaaaccgggc tatttcccat gcacgcctgg ctgccggatg ctcacagtga
    22081 agcgccgagt ccggtcagcg ccctgctctc cgccgtattg ctgaactgcg cgctgttggt
    22141 gctgattcgc tattacatca ttatttgcca ggccatcggc agcgatttcc ccaaccggtt
    22201 gttgctcatc ttcggcatgt tgtcggttgc cgtggcggca tttttcattc tggtacagcg
    22261 ggacattaag cgtctgctgg cgtactccag cgtggagaac atggggctgg tcgcggtggc
    22321 gctaggcatt ggcgggccgc tgggaatttt tgccgcgctg ctgcacacct taaaccacag
    22381 tctggcaaaa acgctgctgt tctgcggttc cggcaatgta ctgctcaagt acggcacgcg
    22441 cgatctcaac gtcgtctgtg ggatgctcaa aatcatgcca tttaccgccg tgctgtttgg
    22501 tggcggtgcg ctggcgctgg cgctggcagg gatgccgccc ttcaacattt ttcttagcga
    22561 atttatgacc gttaccgcag gactggcacg taatcacctg ctgattatcg tcctgctgtt
    22621 attgctgtta acgctggtgc tggcgggcct ggtacggatg gctgcgcggg tgttaatggc
    22681 gaaaccgccg caggccgtta accggggtga tctcggctgg ttgaccacct cgccaatggt
    22741 gattctgctg gtcatgatgc tggcgatggg aacgcatatt ccacaacctg tcatcaggat
    22801 cctggcgggc gcttccacta tagtcctctc agggacgcac gacctgcctg cacaacgtag
    22861 cacctggcat gattttttgc cttcaggcac cgcatctgtt tcggagaaac acagtgaacg
    22921 ttaattcatc gtcaaatcgt ggcgaagcga ttctcgccgc cctgaaaacg cagttccccg
    22981 gcgcggtgct ggatgaagag cgacaaacgc ctgaacaggt caccattacg gtgaaaatca
    23041 atctgctgcc tgacgttgta cattatcttt attatcaaca tgatggctgg cttccagtcc
    23101 tgtttggcaa cgacgagcgg acacttaacg gtcattacgc ggtttattat gccctttcaa
    23161 tggaaggggc cgaaaaatgc tggatcgtgg tgaaggcact ggtcgatgcc gacagtcggg
    23221 agtttccgtc agtcacaccg cgcgtccctg ccgcggtctg gggcgagcga gaaattcgcg
    23281 atatgtacgg gctgattccg gttggcctgc cggatcagcg tcgcctggtg ttgcccgatg
    23341 actggccgga agatatgcat ccgctgcgca aagatgcgat ggattatcga ctgcgccctg
    23401 aaccgacgac agattccgaa acgtatgcgt ttatcaatga gggcaacagc gatgcgcggg
    23461 tgatccctgt cggcccgctg catatcacct cctatgaacc gggtcacttc cgcttgtttg
    23521 tggatggcga gcaaattgtc gatgctgatt accgcctgtt ttatgtccat cgcggcatgg
    23581 agaaactggc agaaacgcgg atgggctaca acgaagtgac cttcttatcg gaccgcgtgt
    23641 gtgggatttg cggttttgcc cacagtgtgg cctataccaa ctcggttgaa aatgcactgg
    23701 ggattgaggt gccgcaacga gcacatacta ttcgctcgat tctgctggaa gtcgagcggc
    23761 tacacagtca tttgctcaac cttggcctct cctgccattt tgttggtttt gataccggct
    23821 ttatgcaatt tttccgcgtg cgggaaaagt cgatgacgat ggcggaattg ctgaccgggt
    23881 cgcgtaaaac ctacggtctg aatctgattg gtggtgttcg ccgcgatatt ctcaaagagc
    23941 aacgtctgca aacgctgaaa ctggtgcgcg agatgcgcgc cgacgtgtcg gagctggtag
    24001 agatgctgct tgctacgccg aatatggaac aacgcactca gggcattggc attctcgacc
    24061 gacaaatcgc ccgtgattat agccctgtag ggccgctgat ccgcgcagtg gttttgcccg
    24121 tgatttgcgc tttgatcacc cctacgccga ctacggcaat attccaaaaa cactgtttac
    24181 ctttaccggc ggcgatgttt tctcccgcgt gatggtccgt gtcaaagaga cgtttgattc
    24241 gctggcaatg ctggaatttg ccctcgacaa catgccggat accccactgc tgaccgaagg
    24301 ctttagctat aaacctcacg cattcgcgct gggctttgtt gaagcgccac gcggtgaaga
    24361 cgtgcactgg agcatgctcg gtgataacca aaaattgttc cgctggcgct gtcgtgccgc
    24421 cacctacgcc aactggccgg tgttgcgtta catgctgcgc ggcaataccg tttctgacgc
    24481 accgctgatt atcggtagcc ttgatccctg ctactcctgt accgaccgtg tgacgctggt
    24541 agatgtgcgc aagcgccagt caaaaaccgt gctgtataaa gagatcgaac gctacggcat
    24601 tgatcgtaac cgttcgccgc tgaagtaagg acagaagatg ctgaagttac tgaaaactat
    24661 tatgcgcgcc ggaaccgcga cggtgaaata tcccttcgcg ccactggagg tcagcccagg
    24721 ctttcgcggg aaaccggacc tgatgcccag ccaatgtatt gcctgcggtg cctgcgcctg
    24781 tgcttgtccg gcaaatgcgc tgactatcca gaccgacgac cagcaaaatt cgcgcacctg
    24841 gcagctctat ctggggcgtt gtatttactg cggacgttgt gaagaagtgt gcccgaccag
    24901 agccatccag cttaccaata actttgaact gaccgtcacc cataaagccg atctctatac
    24961 ccacgcgacg ttccatctac aacgttgcag ccgttgcgaa cgcccgtttg ccccgcaaaa
    25021 aaccgtcgca ctggctgctg aattgttggc acagcaacaa aatgctccac aaaaccgcga
    25081 aatgttatgg gcgcaagcga gcgtctgccc ggagtgcaaa caacgcgcaa cgctgatcaa
    25141 cgacgataca gatgtaccgc tggtggctaa ggagcagcta tgagtccagt gcttacgcaa
    25201 tatgtcagcc agcccatcac gctggacgag caaacgcaaa agatgaagcg gcatttgcta
    25261 caggatatcc gtcgctcggc ttacgtttat cgcatcgatt gcggcggctg caacgcctgt
    25321 gaaatcgaaa tttttgctgc catcaccccc gtattcgacg cagaacgttt tggcattaag
    25381 gttgtttcat caccgcgtca cgccgatatt ttgttattta ctggcgcagt cacccgggcg
    25441 atgcgtatgc ctgcacttcg ggcgtatgag tctgcccccg atcataaaat ctgtgtttcc
    25501 tacggcgcgt gcggtgtcgg cggcggtatt ttccacgatc tctacagcgt ctggggcggc
    25561 agcgacacca ttgtccccat tgatgtttgg atccccggct gcccgccaac accggccgcc
    25621 accattcacg gtttcgccgt ggcgctcggt ttgctgcaac agaagattca cgcggtggat
    25681 tatcgcgatc ccaccggggt gactatgcaa ccgttgtggc cgcagatccc gccatcacag
    25741 cgtatcgcca ttgagcgaga agcgcggcgg ctggcgggct atcgtcaggg gcgagaaatt
    25801 tgcgatcggc tcctgcgcca tttaagcgac gatcctacag gaaatcgggt taacacctgg
    25861 ttgcgcgatg ccgacgatcc acgtctcaat agtatcgttc agcaactctt tcgcgtactc
    25921 cgggggttac atgactgaag agtgcgggga aattgttttc tggacgctgc gaaaaaagtt
    25981 tgtcgccagt agcgacgaga tgccggaaca cagctctcag gtaatgtatt actcgctggc
    26041 tatcggtcat cacgttggcg tgattgattg tctgaatgtc gccttccgct gcccactgac
    26101 ggaatacgaa gattggcttg cactggtcga agaggagcaa gcccgacgta agatgctggg
    26161 ggtgatgact tttggtgaga ttgttattga cgccagccac accgccctgt tgacccgggc
    26221 attcgcgcca ctggcagatg acgcgacgtc tgtgtggcaa gcgcgtagca ttcaattcat
    26281 tcatctgttg gatgaaattg tgctggaacc ggctatctat ctgatggcca gaaaaattgc
    26341 gtgagaagga tttctcatta ataaggactg ttgatggcta tgtcagacga agcgatgttt
    26401 gccccgccgc aaggaataac aattgaagcg gtaaacggaa tgctcgcgga gcggttagcg
    26461 cagaaacacg gcaaggcgtc tttattacgc gccttcatcc cgctgccgcc gccgttcagc
    26521 ccggtacaac ttattgaact gcatgttctc aaaagcaact tctattaccg ctaccatgat
    26581 gatggcagcg atgtgacggc aacaacagag tatcagggcg agatggtcga ttattcgcgt
    26641 tacgccgtcc ttctcggcag tagtggaatg gcggagctac gctttattcg tacccacggc
    26701 agccgtttta ctccccagga ttgcacactg tttaactggc tggcacgcat tatcaccccg
    26761 gttctgcaat catggctcaa tgatgaagca cagcaggtgg cgctgcgttt gctggagaaa
    26821 gatcgcgatc atcatcgggt actggtggat atcactaatg cagtgctgtc acatcttgat
    26881 ctcgacgatc tgatcgctga cgtcgctcgt gagatccatc attttttcgg tctggcttca
    26941 gtcagtatgg tactgggcga tcatcgaaag aacgagaagt tcagcctgtg gtgcagcgat
    27001 ctttctgcct cacattgtgc gtgtctgcca cacaatatgc ctggcgacag tgtattgctg
    27061 acacaaacgc tacaaacccg acaaccgacc ttgacgcacc gtgcagatga tctgtttctc
    27121 tggcaacgcg acccgttatt actcttactt gcatctaacg gctgcgaatc tgcgctcctt
    27181 ataccgctta cctttggcaa ccatacaccg ggtgcattgt tgctggcgca tacctcttcc
    27241 actctcttta gtgaggaaaa ctgccagcta ctacaacaca tagccgatcg catcgctatt
    27301 gccgttggca atgccgatgc ctgccgtaga atgaccgatt tgcaggaaag tttgcagcaa
    27361 gaaaaccacc agcttagcga gcagctcctt tcgaatctgg gcgtcggtga cattatctat
    27421 caaagccagg caatggaaga cctgctccag caggtagata ttgtggcgaa gagcgacagt
    27481 acggtgttga tttgcggtga aaccggaacc ggcaaagagg tgatcgccag agcgatccat
    27541 caacttagcc cgcgacacga caagccgctg gtcaaaatca actgcgctgc catccccgcc
    27601 agtcttctgg aaagtgagtt attcggtcat gacaaagggg cgtttactgg tgcgattaat
    27661 acccatcgtg gtcgttttga aattgccgat ggcggcacgt tgtttctcga tgaaattggc
    27721 gatctgccgt tagaacttca gcctaaactg ctgcgcgtat tgcaggaacg ggagattgag
    27781 cgtctcggcg ggagtagaac gatcccggta aatgtcagag tcattgccgc caccaaccgt
    27841 gatttgtggc aaatggttga agatcgccag tttcgcagcg atctctttta tcgcctgaat
    27901 gtcttcccac tggaattgcc tccgctgcgc gaccgtccgg aagatatccc tcttttagca
    27961 aagcatttca cgcaaaaaat ggcgcgccat atgaatcgtt caattgacgc catcccgacc
    28021 gaggcgctac gccagttgat gtcgtgggac tggccgggca acgtgcgcga gctggaaaac
    28081 gtgattgagc gagcggtact gctgactcgc ggtaacagtc tgaatttaca tcttaatgtc
    28141 cgacaaagcc gtttactgcc gacgctaaat gaagattcag cgcttcgcag ttcaatggcg
    28201 tagttgctgc acccgacgac gccagagaat gacgaagaag aacgtcagcg cattgttcag
    28261 gtattgcgag aaaccaatgg cattgttgcc gggccccgtg gcgcggcgac acgattaggg
    28321 atgaagcgca ccacgctgct gtcacgaatg cagcgtctgg gaatctcggt tcgcgaggtg
    28381 ttgtaatctg cttttgcagg agtatgcatg agaaacaaac tctctttcga cttgcagttg
    28441 agcgccagaa aagcggcaat cgctgaacgg attgccgccc ataaaattgc ccgcagtaaa
    28501 gtgtcggtct ttttaatggc gatgtccgct ggcgtgttta tggcgatcgg atttactttt
    28561 tacctttccg ttatcgccga tgccccgtct tcataggcat taacccatct ggtgggcggc
    28621 ctttgcttta cacttggctt tattttgctg gcggtttgcg gcaccagcct gttcacctcg
    28681 tcggtaatga cggtaatggc aaaaagtcgg ggcgttatta gttggcgaac ttggctgatt
    28741 aacgcacttc tggtggcctg cggtaatctg gcaggtattg cctgtttcag tttgttaatc
    28801 tggttttccg ggctggtgat gagtgaaaac gcgatgtggg gagtcgcgat tttacactgc
    28861 gccgagggca aaatgcatca tacatttact gaatctgtca gcctcggcat tatgtgcaat
    28921 ctgatggttt gcctggcgct gtggatgagt tattgcggac gttcgttatg cgacaaaatc
    28981 gtcgccatga ttttgtccat caccctgttt gtcgccagtg gctttgagca ctgtatcgcc
    29041 aatttgtttg tgattccgtt cgccattgcc attcgccatt tcgcccctac ctccttctgg
    29101 caactggcgc acagtagcgc agacaatttt ccggcactga cggtcagcca ttttattacc
    29161 gccaatctgc tcccggtgat gctgggtaat attatcggcg gtgcagtgct ggtgagtatt
    29221 tgttatcggg ctatttattt acgtcaggaa tcctgaaaaa tcagcccggc gaaacagttc
    29281 gtcgggctga agattattct tgcgcgattt gcccatcggg ccaggcgtgg atcacggctt
    29341 tgatcagcgt cgccaatgga atggcgaaga atacgcccca gaatccccac aaaccaccga
    29401 agatcaccac cgataaaata atcaccagcg gatgcaggtt aaccgcttcg gagaacaaca
    29461 ccggtactag caggttgccg tccagcgcct gaataatcag atacaccgcg aagcagctcc
    29521 agaattcagt acctgcgcca aactggaata gcgccacgcc aaccaccgga atggtcacca
    29581 caaatgcgcc aatgtacgga ataagaaccg agaagccgac cagcactgcc agcagcagcg
    29641 aatagttcag cccgaagagc aagaacccca gccaggtggc gatccccacc acgatcatct
    29701 ccagcacttt gccgcggata tagttggtga tttgttgatt catctccttc cacacctgtc
    29761 ctgccagccc acggttacgc ggcagcaccc ggcgaacggc gttcagcatc tgctctttgt
    29821 ctttcagcag gaagaagacc atcaatggca ccagcaccag atagacggct atggtcagca
    29881 aaccgaccag cgaggcgagg gaaattttca ccaccgaatc gcccatggtc agcatccgac
    29941 tgcgcatatt ttcggccatt gcatcaataa tgcccgcgtc cattaacacc ggatagcggc
    30001 gcggcaacgt ggcggcaaag tcagaaagct tattgagcat ccccggcata tcgcggatta
    30061 agtagatgcc ctgttgccag gcgataggca gtaccacgaa cgccatcagt agcaatatac
    30121 cgacaaaaac caccaataca atcgacgtcg cccagcggcg ggagcagcca atagattgca
    30181 ggcgcacggt tggccattcc agcaaataag ccagcacaat agccaccagc aacggagcaa
    30241 gcaggccact aaagaaaaag ataatgccaa atccggcaac taaaataacc agcaaggcaa
    30301 tcgcttccgg gtcgctaaaa cggcggcgat accattgcat caacatttcg agcataaaga
    30361 ccttccctga acctcaagag cgggattgcg atccgcaatt gtatcgaaat gtcacaaaaa
    30421 agacttcgct ttttatgacg gattcaggaa actgaaaagt catttgagtg ggctaatctt
    30481 cgccgttaca ctcaaaggcg gcgaggtggg aacgatattt cacagtatcg gtcaaatgac
    30541 tatcttcaga aatacaggat agaggttatg ttcaggcagt tgaaaaaaaa cctggttgca
    30601 accctcattg ctgctatgac cattggtcag gtagccccgg cgtttgccga cagcgcagac
    30661 accttgccgg atatgggaac ctccgcagga agcacgcttt ccattggtca ggaaatgcag
    30721 atgggcgact attatgtccg ccagctacgc ggcagcgcgc cgttaattaa tgacccgctg
    30781 ttaacgcaat atattaattc gctggggatg cgtctggttt cgcatgccaa ttcggttaag
    30841 acaccgtttc atttttttct gatcaacaac gacgaaatta acgcctttgc tttctttggc
    30901 ggcaacgtgg tgctgcactc tgccctgttc cgttattccg ataacgaaag tcaactggct
    30961 tcagttatgg cgcacgaaat ctcccacgtc acccaacgtc acctggcgcg agcgatggaa
    31021 gatcaacaac gcagcgcgcc gctgacctgg gtcggcgcgt taggttctat tttactggcg
    31081 atggccagtc cgcaggcggg gatggcggcg ctgaccggta cactggcggg aacgcgccag
    31141 gggatgatca gtttcaccca gcaaaatgaa caggaagcgg accgcattgg tattcaggtg
    31201 ctgcaacgct cgggattcga tccacaggcg atgccgacct tcctggaaaa attgctcgat
    31261 caggcgcgtt actcctcgcg cccgccggaa attttattga ctcacccgtt gccggaaagt
    31321 cgtctggcag atgcccgcaa ccgtgctaat cagatgcgcc cgatggtggt gcagtcgtcg
    31381 gaagatttct atctggcgaa agcgcgcaca ctggggatgt ataattccgg acgtaaccag
    31441 ctcaccagtg atttgctgga tgaatgggcg aaaggaaacg ttcgtcagca acgagcggcg
    31501 caatatggtc gtgctttaca ggcgatggaa gccaataaat acgacgaggc gcgtaaaacg
    31561 ctgcaaccgt tactggcggc agaacctggc aacgcatggt atctcgatct ggctaccgat
    31621 atcgatcttg ggcaaaacaa agccaatgag gcaatcaatc gcctgaaaaa tgcccgcgat
    31681 ttacgcacca atccggtgtt gcagctcaac ctggcgaacg cttatctgca aggcggtcaa
    31741 ccacaagaag cggccaatat tctgaatcgc tacaccttta ataataaaga tgacagcaac
    31801 ggctgggatt tgctggcaca ggcggaagcc gcgctaaata accgcgatca ggagctggct
    31861 gcgcgagcag aaggttatgc gctcgccgga cgactcgatc aggccatttc gctgttgagt
    31921 agcgccagtt cgcaggtgaa attaggcagc ctgcaacaag cgcgttacga tgcgcgcatc
    31981 gaccagttgc gccagctgca gaaacgcttt aagccttata ccaagatgta attcgcatta
    32041 aaggaagaac aatgaccaaa caggtaaaaa tctaccataa cccacgctgt tcaaagagcc
    32101 gggaaacgct gaacctgctg aaagaaaacg gcgtggagcc agaagtggtg ctctaccttg
    32161 agacacccgc cgatgcggca acgctgcgcg atttgctgaa aatgctgggg atgaatagcg
    32221 cccgtgaatt gatgcgccag aaagaggatc tttataaaga actaaacctg gctgacagtt
    32281 cgcttagcga agaggcgctg attcaggcaa tggtggataa tccgaagctg atggagcgcc
    32341 cgattatggt cgcgaatggc aaagcgcgga ttggtcgtcc accggagcag gtactggaaa
    32401 tcgtgggtta agatgtgctc catcgctagt tgaagcacat tgccggatgc gacgcttgcc
    32461 acgtcttatc cggcctacga atactatgga ttaacttcgc gtaggtcagg cgttcgcgcc
    32521 gcatccgaca ataaacacct tatctacaac ttcagaattt ctttcacaaa cggaatggtc
    32581 agcttacgtt gcgcggtaat cgacgcacga tccaactgat ccaacgtcat aaatagcgtg
    32641 cgcatttctc tgtcgagccg cttcagcaag aaacgcccca catcttccgg cagttcaaaa
    32701 ccacgcaaac gcgcgcgtaa ctgtagcgcc tgcaacttat cttcatcaga aagtggctgc
    32761 aatttgtaga tctgccccca gtcgagacgc gacgcgagat ccggtaatcc cagattcaac
    32821 tgccgcggtg gacgatcgcc ggtgatcaac aaccgtgttt tgcccgattc cagaattcga
    32881 ttgtagagat cgaaaatcgc catctcccac aactcatcgc ctgcaataca ctcaatgttg
    32941 tcgatacaga ccagcgacaa atgctccata ccgtcgagca cttccggaac aaaccaggtg
    33001 cgtttatcca gcgggacata gcccaccgca tcgccacgct gcgacaattc cgcgcaagcc
    33061 gcgtgcagca gatggctgcg ccccgcgcct tcgcgtgccc agagatagat gtaaccgcta
    33121 tgttcctgac gcagcacgtt ttgcagcgcg gccagtaaag aggagttatc ccccggccag
    33181 aaacttgcaa aggtttcgtc gtcaggaaga taaagtggca aagagagctg tgccggtgtg
    33241 ttcagagata cctcaaccag gatttcacaa aatcgcgaga agtttaccac agaatcccat
    33301 gatgtttgaa ccgggcagca acactgcccg gtcggtacat tatttgtctg ttatatccgc
    33361 gtcttctgcg tccagcacca cttcttcagg gcgcagcaca ctgatcagtt tgaaaatcaa
    33421 acttaagccg ataccgacga tggtcgccag cgccatacct tttaactccg ccgcgccgat
    33481 gtttaccttc gcgccactga cgccgatgat caaaatcacg gaagtcagga tcaggttctg
    33541 tgctttgttg taatccactt tcgattcgat caaaacacga ataccggaag caccaatgac
    33601 accataaagc agcagcgaaa cgccgcccat caccggcaat gggatcatct ggatagcggc
    33661 agccagttta ccgacgcagg aaagcaggat agcgaaaatc gccgccccgc cgataaccca
    33721 ggtactgtaa acacgggtga tcgccatcac gccaatgttt tctccgtaag tggtatttgg
    33781 cgtagagcca aagaagccgg agatcacggt cgacaggccg ttagcaaaca tcgaacggtg
    33841 cagacctgga tcgcgcagca gatctttttt gacgatatta gccgttacta ccaggtgccc
    33901 tacgtgttcg gcaataacca ctaacgccgc tggcagaata gtcagaatgg caaaccactc
    33961 gaagcgcggc gtatagaggg ttggcagtgc aaaccagtga gcattaataa tcggcgtggt
    34021 atcgacaatt cccattgcga aagagagcgc gtaccccacc agcacgccaa ttaaaatcgg
    34081 gataattgcc aggaaaccac gaaacagcac ggaacctaaa accgtgaccg ccagggtggt
    34141 aatagagatg atgatggttt tggagtttgg cgtttgccct tcagccggga gtaaacccgc
    34201 cataccggca gctacgcccg ccagctccag accgatgacg gcaacgattg cgcccattgc
    34261 cgcaggtgga aacagcacgt ccaaccagcc ggtccccgct ttcttcacga taaaagaaac
    34321 caggcagaac agcacgccgc acataataaa gccgcccagc gcgacttcat accctaacgg
    34381 caacagtaac aataccggtg aaataaaggc aaagctggaa ccaagataag ccggaatttt
    34441 ccctttacag atgaagagat acagcagcgt tccaataccg ttaaataaca gtacagtcgc
    34501 cgggttaata tgaaataaga cgggcaccag gacggttgca ccaaacatgg cgaacaaatg
    34561 ttgcaaacta agcgggattg tctgtaaaag tggcggtctt tcactcaccc cgatagcacg
    34621 gcgcgtcata gtattatcct ctgtattatg tgttataggc gctttactca aaaaaaagcc
    34681 gactcttaaa gtcggcttta attattttta ttctttattt cgtaccaaag attttgtcac
    34741 cggcatcgcc gaggcccgga ataatgtatc cgtgctcgtt cagtccctgg tcaatcgatg
    34801 cagtatacag ttcgacgtcc gggtgcgctt tttccagcgc agcgatacct tctggcgcag
    34861 cgaccagcac cagaactttg atgctgctgc agcccgcttt tttcagcagg tcgatggtcg
    34921 cgataacgga accaccggtt gccagcattg ggtcaacgat cagcgccata cgctcatcga
    34981 tgttagaaac cagtttctgg aagtacggta ccggctccag cgtttcttca ttacggtaca
    35041 taccgacaac gctgatgcgc gcgctcggaa cgttttccag cacaccgtcc atcataccaa
    35101 gacccgcacg cagaattggc acaacggtaa ttttcttacc tttgatctgg tcgatttcta
    35161 ccgggccgtt ccagccttcg atagttactt tttccgtttc gaggtcggcg gtcgcttcgt
    35221 aagtcagcag gctacccact tcggaagcga gttcgcgaaa gcgcttggtg ctgatatctt
    35281 gctcacgcat cagtcccagc ttgtgtttga cgagtgggtg tttgacttcc acgatcttca
    35341 tactctttct cctttgaggg gcagccacaa aaaaatcgac ggattatacc tcctttcttc
    35401 agggcggcaa tattattttc gttgacttta gtcaaaatga taacagtttg agataaagtt
    35461 attttatatt caaatggtta tgaaagaaga ttattccatc cgaaaactaa cctttgccct
    35521 ggcacaagtc ttctttcgcc gcgcgcccgg ggaaaagacg tgcaaaaagg ttgtgtaaaa
    35581 cagtctcgca aacgtttgct ttccctgtta gaattgcgcc gaattttatt tttctaccgc
    35641 aagtaacgcg tggggaccca agcagtgacc gataaaacct ctcttagcta caaagatgcc
    35701 ggtgttgata ttgacgcggg taatgctctg gttggaagaa tcaaaggcgt agtgaagaaa
    35761 acgcgtcgtc cggaagtgat gggcggtctg ggcggcttcg gtgcgctgtg tgcattgccg
    35821 caaaaatatc gtgaacccgt gctggtttcc ggcactgacg gcgtaggtac caagctgcgt
    35881 ctggcaatgg acttaaaacg tcacgacacc attggtattg atctggtcgc catgtgcgtt
    35941 aatgacctgg tggtgcaagg tgcggaaccg ctgtttttcc tcgactatta cgcaaccgga
    36001 aaactggatg ttgataccgc ttcagcggtg atcagcggca tcgcggaagg ttgtctgcaa
    36061 tcgggctgtt ctctggtggg tggcgaaacg gcagaaatgc cggggatgta tcacggtgaa
    36121 gattacgatg tcgcgggttt ctgcgtgggc gtggtagaaa aatcagaaat catcgacggc
    36181 tctaaagtca gcgacggcga tgtgctgatt gcactcggtt ccagcggtcc gcactcgaac
    36241 ggctattcgc tggtgcgcaa aattcttgaa gtcagcggtt gtgatccgca aaccaccgaa
    36301 cttgatggta agccattagc cgatcatctg ctggcaccga cccgcattta cgtgaagtca
    36361 gtgctggagt tgattgaaaa ggtcgatgtg catgccattg cgcacctgac cggcggcggc
    36421 ttctgggaaa acattccgcg cgtattgcca gataataccc aggcagtgat tgatgaatct
    36481 tcctggcagt ggccggaagt gttcaactgg ctgcaaacgg caggtaacgt tgagcgccat
    36541 gaaatgtatc gcaccttcaa ctgcggcgtc gggatgatta tcgccctgcc tgctccggaa
    36601 gtggacaaag ccctcgccct gctaaatgcc aacggtgaaa acgcgtggaa aatcggtatc
    36661 atcaaagcct ctgattccga acaacgcgtg gttatcgaat aatgaatatt gtggtgctta
    36721 tttccggcaa cggaagtaat ttacaggcaa ttattgacgc ctgtaaaacc aacaaaatta
    36781 aaggcaccgt acgggcagtt ttcagcaata aggccgacgc gttcggcctt gaacgcgccc
    36841 gccaggcggg tattgcaacg catacgctca tcgccagcgc gtttgacagt cgtgaagcct
    36901 atgaccggga gttgattcat gaaatcgaca tgtacgcacc cgatgtggtc gtgctggctg
    36961 gttttatgcg cattctcagc ccggcgtttg tctcccacta tgccgggcgt ttgctgaaca
    37021 ttcacccttc tctgctgccg aaatatcccg gattacacac ccatcgtcag gcgctggaaa
    37081 atggcgatga agagcacggt acatcggtgc atttcgtcac cgatgaactg gacggtggcc
    37141 cggttatttt acaggcgaaa gtcccggtat ttgctggtga tacggaagat gacatcaccg
    37201 cccgcgtgca aacccaggaa cacgctattt atccactggt gattagctgg tttgccgatg
    37261 gtcgtctgaa aatgcacgaa aacgccgcgt ggctggatgg tcaacgtctg ccgccgcagg
    37321 gctacgctgc cgacgagtaa tgcccccgta gttaaagcgc cagctctgcc gctggcgttt
    37381 ttcaattcac ctgtaaatcg caagctccag cagttttttt cccccttttc tggcatagtt
    37441 ggacatctgc caatattgct cgccataata tccaggcagt gtcccgtgaa taaaacggag
    37501 taaaagtggt aatgggtcag gaaaagctat acatcgaaaa agagctcagt tggttatcgt
    37561 tcaatgaacg cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt gaaaggatgc
    37621 gtttcctggg gatctattcc aataaccttg atgagttcta taaagtccgc ttcgctgaac
    37681 tgaagcgacg catcattatt agcgaagaac aaggctccaa cgctcattcc cgccatttac
    37741 tgggcaaaat tcagtcccgg gtgctgaaag ccgatcagga attcgacggc ctctacaacg
    37801 agctattgct ggagatggcg cgcaaccaga tcttcctgat taatgaacgc cagctctccg
    37861 tcaatcaaca aaactggctg cgtcattatt ttaagcagta tctgcgtcag cacattacgc
    37921 cgattttaat caatcctgac actgacttag tgcagttcct gaaagatgat tacacctatc
    37981 tggcggtgga aattatccgt agcgatagca tccgttacgc gctgctggag atcccatcag
    38041 ataaagtgcc gcgctttgtg aatttaccgc cagaagcgcc gcgtcgacgc aagccgatga
    38101 ttcttctgga taacattctg cgttactgcc ttgatgatat tttcaaaggc ttctttgatt
    38161 atgacgcgct gaatgcctat tcaatgaaga tgacccgcga tgccgaatac gatttagtgc
    38221 atgagatgga agccagcctg atggagttga tgtcttccag tctcaagcag cgtttaactg
    38281 ctgagccggt gcgttttgtt tatcagcgcg atatgcccaa tgcgctggtt gaagtgttac
    38341 gcgaaaaact gactatttcc cgctacgact ccatcgtccc cggcggtcgt tatcataatt
    38401 ttaaagactt tattaatttc cccaatgtcg gcaaagccaa tctggtgaac aaaccactgc
    38461 cgcgtttacg ccatatttgg tttgataaag cccagttccg caatggtttt gatgccattc
    38521 gcgaacgcga tgtgttgctc tattatcctt atcacacctt tgagcatgtg ctggaactgc
    38581 tgcgtcaggc ttcgttcgac ccgagcgtac tggcgattaa aattaacatt taccgcgtgg
    38641 cgaaagattc acgcatcatc gactcgatga tccacgccgc acataacggt aagaaagtca
    38701 ctgtggtggt tgagttacag gcgcgtttcg acgaagaagc caacattcac tgggcgaagc
    38761 gcctgaccga agcaggcgtg cacgttatct tctctgcgcc ggggctgaaa attcacgcca
    38821 aactgttcct gatttcacgt aaagaaaacg gtgaagtggt ccgttacgca cacatcggga
    38881 ccgggaactt taacgaaaaa accgcgcgtc tttatactga ctattcgttg ctgaccgcag
    38941 atgcgcgtat caccaacgaa gtacggcggg tatttaactt tattgaaaac ccataccgcc
    39001 cggtgacatt tgattattta atggtgtcac cgcaaaactc tcgccgtctg ttatatgaaa
    39061 tggtagaccg cgaaatcgcc aacgcgcagc aagggctgcc cagtggtatc accctgaagc
    39121 taaataacct tgtcgataaa ggcctggttg atcgtctgta tgcggcctcc agctccggcg
    39181 taccggttaa tctgctggtt cgcggaatgt gttcgctgat ccccaatctg gaaggcatta
    39241 gcgacaacat tcgtgccatc agtattgttg accgttacct tgaacatgac cgggtttata
    39301 tttttgaaaa tggcggcgat aaaaaggtct acctttcttc cgccgactgg atgacgcgca
    39361 atattgatta tcgtattgaa gtggcgacac cgctgctcga tccgcgcctg aagcagcggg
    39421 tgctggacat catcgacata ttgttcagcg atacggtcaa agcacgttat atcgataaag
    39481 aactcagtaa tcgctacgtt ccccgcggca atcgccgcaa agtacgggcg cagttggcga
    39541 tttacgacta catcaaatca ctcgaacaac ctgaataacc ctatgccaat acacgataaa
    39601 tcccctcgtc cgcaggagtt tgctgcggtc gatcttggtt caaacagttt tcacatggtc
    39661 atagcccgtg tggtagatgg tgccatgcag attattggcc gcctgaaaca gcgggtgcat
    39721 ctggcggacg gcctggggcc agataatatg ttgagtgaag aggcaatgac gcgcggtttg
    39781 aactgtctgt cgctgtttgc cgaacggcta caagggtttt ctcctgccag cgtctgtata
    39841 gtcggcaccc atacgctgcg tcaggcgctg aacgccactg actttctgaa acgcgcggaa
    39901 aaggtcattc cctacccgat tgaaattatt tccggtaatg aagaagcccg tctgattttt
    39961 atgggcgtgg aacataccca accggaaaaa ggtcgcaaac tggttattga tattggcggc
    40021 ggatctacgg aactggtgat tggtgaaaat ttcgaaccta ttctcgttga aagccgccgg
    40081 atgggttgtg tcagctttgc ccagctttat tttcctggcg gggtcatcaa taaagagaat
    40141 tttcagcgcg ctcgcatggc ggcagcacaa aaactggaaa ctttaacctg gcaattccgt
    40201 attcagggct ggaacgttgc aatgggcgct tccggtacca taaaagccgc ccatgaagtg
    40261 ttaatggaaa tgggcgagaa agacgggata attaccccgg aacgtctgga aaaactggta
    40321 aaagaaattt tacgtcaccg taatttcgca tcgctgagtt taccgggtct ttccgaagag
    40381 cggaaaacag tcttcgttcc tggactggcg attttatgtg gtgtgtttga tgctttagcc
    40441 atccgtgaac tgcgcctttc tgacggggcg ttacgcgaag gcgtactgta tgaaatggaa
    40501 ggacgtttcc gtcatcagga tgtgcgtagt cgcaccgcca gcagcctcgc caaccagtat
    40561 cacatagaca gcgaacaggc ccgacgagtg ctggatacca ctatgcaaat gtacgaacag
    40621 tggcgggaac agcaaccgaa gctggcgcat ccgcaactgg aggcgctact gcgatgggcc
    40681 gccatgctgc atgaggtcgg gttgaatatc aaccacagcg gtttgcatcg ccactccgct
    40741 tatattctgc aaaacagtga cttgccgggt tttaatcagg aacagcagct gatgatggcg
    40801 acactggtgc gctatcaccg taaagcgatt aagctcgacg atctgccgcg ctttaccttg
    40861 tttaagaaga aacagttcct gccactgata cagctattgc gccttggcgt attactaaac
    40921 aatcaacgtc aggcaaccac cacaccgcca acattgacac tgattactga tgacagtcac
    40981 tggacactgc gtttcccgca tgactggttt agtcagaatg cgctggtact gcttgatctg
    41041 gaaaaggagc aagaatactg ggaaggcgtg gctggctggc ggttgaaaat tgaagaagaa
    41101 agtaccccag aaatcgccgc ttaatgcttt gcgggccgac gagaaatgtc ggcccgcatt
    41161 attcaggcac tttcgcgaat gggttcgatt tcattcagcg tatcaattaa cggttgcggc
    41221 ttaccaataa gataaccctg catataatcg atccccaaag agagcaccgc ctcgcggatc
    41281 tcttcgtttt caacgtactc tgccactacc cgcattttct tcattcgcgc cagatggcaa
    41341 atcgacgcca caatctgata atccagacta tttgacacaa tattgctgat aaagctgccg
    41401 tcaattttaa gcagatcggc attcacattt ttaagccgtg catagctggc gtagccggtg
    41461 ccgaaatcat caatcgcaat ctggcagcct aattcctgaa gatgctgcaa ggtgatttgc
    41521 gcctgcttaa cattggtcag agcattactt tcggtgactt caaaaataag ttgccacgct
    41581 tcaatctgat atttagccag caactgactg acttcaacag gaaaacgagc ctgacatacc
    41641 gaggttggag acagattaat agcaaaacgg tgagcgggca tcttcgctct gttttcagcc
    41701 ataaattgca gcgtatgctc aatgacccac atgtcgatac tcgacgataa accaaattcg
    41761 tgcgcgaccg gcaagaagct accggggccg atcagttcat cattctcacc tttcatgcgc
    41821 agaagaattt catggtaaac atcaccacgc ataccggtaa tcggctgggc catcaggaaa
    41881 aaatggttgt gttccagcgc ctgctgtagt cgattcatca tcgcgacttt atctttcaat
    41941 tcgcgttgca aatacattgc cccgcaacgc tgcatatttt ccggggcgtt ggtcacgatg
    42001 gaaagttcgg cgaccgtatt tagctctccc agcagcaggt agatatgatt cactggcgag
    42061 cgcacatagc agtaactgac gccaatctgc ggttgcatcg gcatcccatc ccaaaagaaa
    42121 cgaaattgct tgagatgact atccagtgcg gtaatgcgct cctggtgcga ttctgtattc
    42181 agtcgcagcg cgagatcgtt acccgaaagc tgataaacat cttcacccgg ttccagcaag
    42241 ggtgacagcc agtgagaaag tttttgcttg t
//
LOCUS       DAETQL010000030        41056 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.030, whole genome shotgun sequence.
ACCESSION   DAETQL010000030 DAETQL010000000
VERSION     DAETQL010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41056
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.030"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..160
                     /locus_tag="K0W65_001714"
     CDS             <1..160
                     /locus_tag="K0W65_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI3095008.1"
                     /translation="EIEQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVS
                     GGGVQELN"
     gene            389..979
                     /gene="uidR"
                     /locus_tag="K0W65_001715"
     CDS             389..979
                     /gene="uidR"
                     /locus_tag="K0W65_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HBI3095009.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1367..3070
                     /gene="uidA"
                     /locus_tag="K0W65_001716"
     CDS             1367..3070
                     /gene="uidA"
                     /locus_tag="K0W65_001716"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBI3095010.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLSIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3076..4101
                     /gene="uidB"
                     /locus_tag="K0W65_001717"
                     /pseudo
     CDS             3076..4101
                     /gene="uidB"
                     /locus_tag="K0W65_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4140..5405
                     /gene="uidC"
                     /locus_tag="K0W65_001718"
     CDS             4140..5405
                     /gene="uidC"
                     /locus_tag="K0W65_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBI3095011.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5631..7139)
                     /locus_tag="K0W65_001719"
     CDS             complement(5631..7139)
                     /locus_tag="K0W65_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBI3095012.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7240..8415)
                     /gene="manA"
                     /locus_tag="K0W65_001720"
     CDS             complement(7240..8415)
                     /gene="manA"
                     /locus_tag="K0W65_001720"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBI3095013.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8614..10260
                     /gene="fumB"
                     /locus_tag="K0W65_001721"
     CDS             8614..10260
                     /gene="fumB"
                     /locus_tag="K0W65_001721"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBI3095014.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10403..11806
                     /gene="fumC"
                     /locus_tag="K0W65_001722"
     CDS             10403..11806
                     /gene="fumC"
                     /locus_tag="K0W65_001722"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBI3095015.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11803..12732)
                     /gene="tus"
                     /locus_tag="K0W65_001723"
     CDS             complement(11803..12732)
                     /gene="tus"
                     /locus_tag="K0W65_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBI3095016.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12808..14109)
                     /gene="rstB"
                     /locus_tag="K0W65_001724"
     CDS             complement(12808..14109)
                     /gene="rstB"
                     /locus_tag="K0W65_001724"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBI3095017.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14288..15607)
                     /locus_tag="K0W65_001725"
     CDS             complement(14288..15607)
                     /locus_tag="K0W65_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBI3095018.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15832..16575)
                     /gene="rstA"
                     /locus_tag="K0W65_001726"
     CDS             complement(15832..16575)
                     /gene="rstA"
                     /locus_tag="K0W65_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBI3095019.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16704..17039
                     /locus_tag="K0W65_001727"
     CDS             16704..17039
                     /locus_tag="K0W65_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBI3095020.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17036..17758)
                     /gene="folM"
                     /locus_tag="K0W65_001728"
     CDS             complement(17036..17758)
                     /gene="folM"
                     /locus_tag="K0W65_001728"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBI3095021.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17795..19177)
                     /locus_tag="K0W65_001729"
     CDS             complement(17795..19177)
                     /locus_tag="K0W65_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI3095022.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19363..20307)
                     /locus_tag="K0W65_001730"
     CDS             complement(19363..20307)
                     /locus_tag="K0W65_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBI3095023.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20831..22363
                     /gene="pntA"
                     /locus_tag="K0W65_001731"
     CDS             20831..22363
                     /gene="pntA"
                     /locus_tag="K0W65_001731"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBI3095024.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22374..23762
                     /gene="pntB"
                     /locus_tag="K0W65_001732"
     CDS             22374..23762
                     /gene="pntB"
                     /locus_tag="K0W65_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBI3095025.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23787..24821)
                     /gene="tqsA"
                     /locus_tag="K0W65_001733"
     CDS             complement(23787..24821)
                     /gene="tqsA"
                     /locus_tag="K0W65_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBI3095026.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25232..25597
                     /gene="mdtJ"
                     /locus_tag="K0W65_001734"
     CDS             25232..25597
                     /gene="mdtJ"
                     /locus_tag="K0W65_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBI3095027.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25584..25913
                     /gene="mdtI"
                     /locus_tag="K0W65_001735"
     CDS             25584..25913
                     /gene="mdtI"
                     /locus_tag="K0W65_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBI3095028.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25952..26773)
                     /locus_tag="K0W65_001736"
     CDS             complement(25952..26773)
                     /locus_tag="K0W65_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBI3095029.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVVLRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26873..26956)
                     /locus_tag="K0W65_001737"
     CDS             complement(26873..26956)
                     /locus_tag="K0W65_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095030.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27049..27384)
                     /locus_tag="K0W65_001738"
     CDS             complement(27049..27384)
                     /locus_tag="K0W65_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBI3095031.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27781..29034)
                     /locus_tag="K0W65_001739"
                     /pseudo
     CDS             complement(27781..29034)
                     /locus_tag="K0W65_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29141..30061
                     /locus_tag="K0W65_001740"
     CDS             29141..30061
                     /locus_tag="K0W65_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3095032.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAELAKEPFVFFDPHVGTGLYDDI
                     LGLMRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDA
                     VSEMWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30196..31416
                     /gene="mlc"
                     /locus_tag="K0W65_001741"
                     /gene_synonym="dgsA"
     CDS             30196..31416
                     /gene="mlc"
                     /locus_tag="K0W65_001741"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBI3095033.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31541..32236
                     /gene="bioD"
                     /locus_tag="K0W65_001742"
     CDS             31541..32236
                     /gene="bioD"
                     /locus_tag="K0W65_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBI3095034.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32189..33481)
                     /gene="clcB"
                     /locus_tag="K0W65_001743"
     CDS             complement(32189..33481)
                     /gene="clcB"
                     /locus_tag="K0W65_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBI3095035.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISQTLHRDSIYRQHTAQHS"
     gene            complement(33640..34254)
                     /gene="dmsD"
                     /locus_tag="K0W65_001744"
     CDS             complement(33640..34254)
                     /gene="dmsD"
                     /locus_tag="K0W65_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBI3095036.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34297..35151)
                     /locus_tag="K0W65_001745"
     CDS             complement(34297..35151)
                     /locus_tag="K0W65_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBI3095037.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35153..35770)
                     /gene="dmsB"
                     /locus_tag="K0W65_001746"
     CDS             complement(35153..35770)
                     /gene="dmsB"
                     /locus_tag="K0W65_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBI3095038.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35781..37819)
                     /locus_tag="K0W65_001747"
                     /pseudo
     CDS             complement(35781..37819)
                     /locus_tag="K0W65_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37880..38989)
                     /locus_tag="K0W65_001748"
     CDS             complement(37880..38989)
                     /locus_tag="K0W65_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBI3095039.1"
                     /translation="MVNVSHIRTLSSVESTTSSPSIRLTVESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38972..40561
                     /locus_tag="K0W65_001749"
     CDS             38972..40561
                     /locus_tag="K0W65_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBI3095040.1"
                     /translation="MTDINHDNTAYPSSYFSGSPYLYGQVYTPSPDTRLTQINLLGVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNHTDYSTIGDSTSILKNEQGDRPVVFTQE
                     RQPEVTICSFSELVSSLLAEKVQTLKSSSYKDLSSSLNTVSRFLPEDMDLMSRSEWLA
                     VRDAMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDVDSKRRAFT
                     DEEMERLLVRVESEYQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGACGLDLKSFLEWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDATDVSLHSLRHWLATRMKERGANLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLAEVLKTALL"
BASE COUNT        10424 a         9965 c        10521 g        10146 t
ORIGIN      
        1 agaaattgaa caaaaaatgt tacagcacac gccgatcaga cgtctgggcc aaccgcaaga
       61 tattgctaac gcggcgctgt tcctttgctc gcctgcagcc agctgggtaa gcggacaaat
      121 tctcaccgtc tccggtggtg gggtacagga gctcaattaa tacactaacg aaccggtaaa
      181 cggtcgtatg tgttgtttac cgggatagcg tgtgcaattc atcaacgtct ctgctaaaca
      241 actggcagcc aaatcacggc tattggttaa ccaattgctg ggtgaaaagt atacgaatag
      301 agtgtacctt cgcactattc aacagcaatg ataggcgctc acctgacaac gcggtaaact
      361 agttattcac gctaactata atggtttaat gatggataac atgcagactg aagcacaacc
      421 gacacggacc cggatcctca atgctgccag agagattttt tcagaaaatg gatttcacag
      481 tgcctcgatg aaagccatct gtaaatcttg cgccattagt cctgggacgc tctatcacca
      541 tttcatctcc aaagaagcct tgattcaggc gattatctta caggaccagg agagggcgct
      601 ggctcgtttc cgggaaccga ttgaagggat tcattttgtt gactatatgg tcgagtctat
      661 tgtctctctc acccatgaag cctttgggca acgggcgctg gtggtcgaaa ttatggcgga
      721 agggatgcgt aacccacagg tcgccgccat gcttaaaaat aagcatatga cgatcactga
      781 atttgttgcc cagcggatgc gtgatgccca gcaaaaaggc gagataagcc cagacatcaa
      841 cacggcaatg acttcacgtt tactgctgga tctgacctac ggtgtactgg ccgatatcga
      901 agcggaagac ctggcgcgtg aagcgtcgtt tgctcaggga ttacgcgcga tgattggcgg
      961 tatattaacc gcatcctgat cctctcattt cgcgggctgg tgataactgt gcccgcgttt
     1021 catatcgtaa tttctctgtg caaaaattat cctccccgtg ttcggagaat tcccccccaa
     1081 aatattcacc gtagccatat gtcatgagag tttatcgttc ccaacactct cgaacgaacg
     1141 ttcgattgct tattttatgg cttctgtcaa cgctgtttta aagattaatg cgatccatat
     1201 cacgctgtgg gtattgcagt ttttggtttt ttgatcgcgg tgtcagttct ttttatttcc
     1261 atttctcttc catgggtttc tcacagataa ctgtgtgcaa cacagaattg gttaactaat
     1321 cagattaaag gttgaccagt atttttatct taatgaggag tcccttatgt tacgtcctgt
     1381 agaaacccca acccgtgaaa tcaaaaaact cgacggcctg tgggcattca gtctggatcg
     1441 cgaaaactgt ggaattgatc agcgttggtg cgaaagcgcg ttacaagaaa gccgggcaat
     1501 tgcagtgcca ggcagtttta acgatcagtt cgccgatgca gatattcgta attatgtggg
     1561 caacgtctgg tatcagcgcg aagtctttat accgaaaggt tgggcaggcc agcgtatcgt
     1621 gctgcgtttc gatgcggtca ctcattacgg caaagtgtgg gtcaataatc aggaagtgat
     1681 ggagcatcag ggcggctata cgccatttga agccgatgtc acgccgtatg ttattgccgg
     1741 gaaaagtgta cgtatcaccg tttgtgtgaa caacgaactg aactggcaga ctatcccgcc
     1801 gggaatggtg attaccgacg aaaacggcaa gaaaaagcag tcttacttcc atgatttctt
     1861 taactatgcc gggatccatc gcagcgtaat gctctacacc acgccgaaca cctgggtgga
     1921 cgatatcacc gtggtgacgc atgtcgcgca agactgtaac cacgcgtctg ttgactggca
     1981 ggtggtggcc aatggtgata tcagcgttga actgcgtgat gcggatcaac aggtggttgc
     2041 aactggacaa ggcaccagcg ggactttgca agtggtgaat ccgcacctct ggcaaccggg
     2101 tgaaggttat ctctatgaac tgtgcgtcac agccaaaagc cagacagagt gtgatatcta
     2161 cccgctgcgc gtcggcatcc ggtcagtggc agtgaagggc gaacagttcc tgattaacca
     2221 caaaccgttc tactttactg gctttggtcg tcatgaagat gcggatttgc gaggcaaagg
     2281 attcgataac gtgctgatgg tgcacgatca cgcattaatg gactggattg gggccaactc
     2341 ctaccgtacc tcgcattacc cttacgctga agagatgctc gactgggcag atgaacatgg
     2401 catcgtggtg attgatgaaa ctgcagctgt cggctttaac ctctctttaa gcattggttt
     2461 cgaagcgggc aacaagccga aagaactgta cagcgaagag gctgtcaacg gggaaactca
     2521 gcaggcgcac ttacaggcga ttaaagagct gatagcgcgt gacaaaaacc acccaagcgt
     2581 ggtgatgtgg agtattgcca acgaaccgga tacccgtccg caaggtgcac gggaatattt
     2641 cgcgccactg gcggaagcaa cgcgtaaact cgacccgacg cgtccgatca cctgcgtcaa
     2701 tgtaatgttc tgcgacgctc acaccgatac catcagcgat ctctttgatg tgctgtgcct
     2761 gaaccgttat tacggttggt atgtccaaag cggcgatttg gaaacggcag agaaggtact
     2821 ggaaaaagaa ctgctggcct ggcaggagaa actgcatcag ccgattatca tcaccgaata
     2881 cggcgtggat acgttagccg gcctgcactc aatgtacacc gacatgtgga gtgaagagta
     2941 tcagtgtgca tggctggata tgtatcaccg cgtctttgat cgcgtcagcg ccgtcgtcgg
     3001 tgaacaggta tggaatttcg ccgattttgc gacctcgcaa ggcatattgc gcgttggcgg
     3061 taacaattga cctacatggg cctcgggctt tgctacagcc tggtgaatat tccttatggt
     3121 tcacttgcta ccgcgatgac ccaacaacca caatctcgcg cccgtctggg cgcggctcgt
     3181 gggattgccg cttcattgac ctttgtatgc ctggcatttc tgataggacc gagcattaag
     3241 aactccagcc cggaagagat ggtgtcggta taccatttct ggacgattgt gctggtgatt
     3301 gccggaatgg tgctttactt catctgcttc aaatcgacgc gtgaaaatgt ggtacgtatc
     3361 gtggcgcagc cgtcattgaa gatcagtttg caaaccctga aacggaatcg cccgctgttt
     3421 atgttgtgca tcggtgcgct gtgtgtgctg atttcgacct tcgcggtcag cgcctcgtcg
     3481 ttgttctacg tgcgctatgt gttaaatgat accgggctgt tcactgtgct ggtactggtg
     3541 caaaacctgg ttggtactgt ggcatcggca ccgcttgtgc cggggatggt cgcgaggatc
     3601 ggtaaaaaga ataccttcct gattggcgct ttgctgggaa cctgcggtta tctgctattc
     3661 ttctgggttt ccgtctggtc actgccggtg gcgttggttg cgttggccat cgcttcaatt
     3721 ggtcagggcg ttactatgac tgtgatgtgg gcactggaag ctgataccgt agagtacggt
     3781 gaatacctga ccggcgtgcg aattgaaggg ctcacctatt cactattctc atttacccgt
     3841 aaatgcggtc aggcaatcgg aggttcaatt cctgccttta ttttgggatt aagcggatat
     3901 atcgccaatc aggtgtaaac gccggaagtt attatgggca tccgcacatc aattgcctta
     3961 gtaccttgcg gatttatgct actggcattc gttattatct ggttttatcc gctcacggat
     4021 aaaaaattca aagaaatcgt ggttgaaatt gataatcgta aaaaagtgca gcagcaatta
     4081 atcagcgata tcactaatta atattcaata aaaataatca gaacatcaaa ggtgcaacta
     4141 tgagaaaaat agtggccatg gccgttattt gcctgacggc tgcctctggc cttacctctg
     4201 cttacgcggc gcaactggct gacgatgaag cgggactacg catcagactg aaaaacgaat
     4261 tgcgcagggc tgataagccc agtgctggcg cgggaagaga tatttacgca tgggtacagg
     4321 gaggattgct cgatttcaat agtggttatt attccaatat tgttggcgtt gaaggcggag
     4381 cgtattatgt ttataaatta ggtgctcgtg ctgatatgag tacccggtgg tatcttgatg
     4441 gtgataaaag ttttggcttt gccctggggg cagtaaaaat aaaacccagt gaaaatagcc
     4501 tgcttaaatt aggtcgcttc gggacggatt atagttatgg tagcttacct tatcgtattc
     4561 cgttaatggt tggcagttcg caacgtacat taccgacagt ttctgaagga gcattaggtt
     4621 attgggcttt aacgccaaat attgatctgt ggggaatgtg gcgttcacga gtatttttat
     4681 ggactgattc aacaaccggt attcgtgatg aaggggtgta taacagccag acgggaaaat
     4741 acgataaaca tcgcgcacgt tcttttttag ccgccagttg gtatgatgat accagtcgct
     4801 attctctggg ggcatcggta cagaaagatg tttccaatca gatacaaagt attctcgaga
     4861 aaagcatacc gctcgacccg aattatacgt tgaaagggga gttgctcggc ttttacgcgc
     4921 agctcgaagg tttaagtcgt aataccagcc agcccaatga aacggcgttg gttagtggac
     4981 aattgacctg gaatgcgccg tggggaagcg tatttggcag tggtggttat ttgcgccatg
     5041 caatgaatgg tgccgtggtg gataccgaca ttggctatcc cttttcatta agtcttgatc
     5101 gtaaccgtga aggaatgcag tcctggcaat taggtgccaa ctaccgtgta actccgcaat
     5161 ttacgctgac atttgcaccg attgtgactc gcggctatga atccagtaaa cgagatgtgc
     5221 ggattgaagg cgcgggtatc ttaggtggta tgaactatcg ggtcagcgaa gggccgttac
     5281 aagggatgaa tttctttctt gctgccgata aagggcggga aaagcgcgat ggcagtacgc
     5341 tgggcgatcg cctgaattac tgggatgtga aaatgagtat tcagtatgac tttatgctga
     5401 agtaagaaat aacgccggag agaaaaacct ccggcgtttc agattgttga caaagtgcgc
     5461 gttttttatg ccggatgcgg cgtaaacgcc ttatccagcc tacaaaaact cataaattca
     5521 aagtgttgca ggaaaaggta ggcctgataa gcgtagcgca tcaggcaatc tctggtttgt
     5581 tttcagatga aaacgccgga gtgaaaattc tccggcgttt tggccgtgaa ttactgctgc
     5641 ggaattgccg gtacagccgg aacgttaaga gctggcatcg caaacatgcc aacaaaatct
     5701 tctaacgaca ttttctgccc atttaacgtt atctgaccgt tagcatattg caggctggtg
     5761 gtgatggtat tgtcctgcaa ggtggtcaga cggaacatct gccccattgc tgatgcacct
     5821 tcaacttgct gtttcgccag ttttttcgct tgatcttcct gataaccttc cagcttcgct
     5881 acctgagtca tcaactcagt tgccatatcc accggaatgg tcagtttcgc atccagagat
     5941 ttaaccgaac gatctacttc ctgcgccagc gtttgcggcg cttctttagt cgttgccgga
     6001 tctttcagga acagcgacag attcagcgca ctttcaccct gactgttttt ccagcttagc
     6061 ggcgcgatag taatcaccgg atcgcctttc aacatcagcg gcagggcgct aaagaaggct
     6121 tccgtcactt tctcctgata aagttcggga ttgttggcaa tttctggctg tgccagcagc
     6181 gcctgagttt gcgcgttata ttgctggcta aactgatgcc aggcttcacc atcaatctgg
     6241 ccgactttta aagtcagctt gccgctgccc agatcctgat tctgtacctt caggctgttt
     6301 agcgagtaat ccagttggct attgatcgtt ttaccgtcat tgaccagatc cgatttaccg
     6361 ctgatctcca tgccttccag cagtgccagt tctttgcctt ccactgaaat ggtcattttt
     6421 tccagtgaca gtttttggtt tcctacgcgc tcaccaaaac ttcccagcgt gctggaaccg
     6481 tcggttttca gattattaaa ggtcaactgc actttctggt tgtattcgtt aactgcgtct
     6541 atccgaccac tttgcgcctc tccggaaagg gagatggctt tgccgtctct gtcagcattt
     6601 aactggaact cgccgccgct aaaggcgact ttttcatcct tttgctcgta attcagtgga
     6661 ttgagcgaaa tatcggaact ggaatcaccg ctgtaaccaa tgcgcgagtt aatctcaaaa
     6721 ggcgtttcac cttttgccat atcaaacagt ggtttgctta cttcgttatt aaccagcgtg
     6781 gtttgaattg atgccatcga cgggatcagg ttcagttttt taagctgggc aagcgggaag
     6841 ggaccatgat caaccgattc gttgaagatg acgctctgac cgcttttaat ccacggattt
     6901 tctttcccgg caatgggttt caccaacagt tgcaactggc tgctgaatac gccgcgatga
     6961 tagttttgat aactcacttc caggttggat tcaggagctg tcagtttgag ttgcgcgttc
     7021 gcctgcgcga ccatgtcttc gagatgggtt tcaatcttct tgcctgtata ccatgcgccg
     7081 cctgtccaga ctacgcctag cgcaacaatg acgcctaccg ctaccagcga tttattcata
     7141 atgattatcc ataaaatgaa atcaggcgga ctggccgcct gaaggtgtta taagccttta
     7201 ataagcttag caagagatgt taattttttc agtaagctct tacagcttgt tgtaaacacg
     7261 cgctaaacgg ccgtggcctt tgacagtcac cggtgattcg ttggcggcaa taaacgctga
     7321 ttcacccggt ttaagctgta actgctgaga acctttccac aacgttgcat cgccttcgac
     7381 gcagaacaaa atggcggcac tctgctggct gatggtggtt tctttatcac taaggtcatg
     7441 cagcgagaag gcaaaatcat ccacaggaat cgggaagtcc agttctgcac cttgtttcac
     7501 cggctgggtc aacaactggt tagccggttt ggcttcgaat ttcacattgg caaccagttc
     7561 cggaatatca atgtatttag gcgtcagacc tgcacgcagc acgttatcgg agttcgccat
     7621 cacttccagc gccacgcctt gcaggtaagc gtgcggtgtt tcagcgaaca ggaacatcgc
     7681 ttcgccagga ttcaatttca ccacattcag taacagcggg gagaacaggc cgctgtcttc
     7741 cgggtaaaat tcagaaatta aacgaatcgt ttgccacggt tcaccctgct gactgtcgag
     7801 cgccgatttt aaaatcgcca gcgcgcggga tttttcttca ccctgcatat tcaacaggtt
     7861 ggcgaacagt tcgcttaaac gttcggcatg tggctgttgt aaaaagtgag caatcgccgg
     7921 atgtgcaccg gcgaccggct ggagtaggga gacaatctcg gaaaattcac gaaacgcgtt
     7981 catcgcaagg aaaggcgtca gcgcaaaaac cagctccggc ttgtggttag gatctttata
     8041 gttacgctcg gcggcatcca tcgggatacc tgcggcattt tctttggcaa aaccgatttc
     8101 agaattgtgt ttgtttggat gaacctgaat ggagagtggc tgtgctgcgc ataatacttt
     8161 gaacaggaaa ggcagttcgc caaagcgttt ggcaacggcc tctccgagca gagtcgattt
     8221 atcactctca atcacatcac gcagtgaaac gatatctccg gcggcattct gcactcgtga
     8281 actgcttttc ggatgtgcgc ccatccacag ctcggccatc ggctggctgg acggattttc
     8341 cataccataa agttcagtca acgccgtttt gctaccccag gcatagtttt gcactgagtt
     8401 aatgagtttt tgcatgatca atccctgttt taatgtggaa attaatcccg ctattaaagc
     8461 aagaatccta cgggaagtaa cctggagccg caaaaagtcg tactagtctc agtttttgtt
     8521 aaaaaactgt gtaggatatt gttactcgct tttaacaggg caacggaaca cccgcccaga
     8581 gcataaccaa accaggcagt aagtgagaga acaatgtcaa acaaaccctt tcattatcag
     8641 tctccttttc cactcaaaaa aaatgatact gagtattacc tgctaaccag tgaacacgtt
     8701 agcgtatctg aatttgaagg gcaggagatt ttgaaagtcg cacccgaagc gttaactctg
     8761 ttggcgcgtc aggcgtttca tgatgcgtcg tttatgctgc gtccggctca ccaacaacag
     8821 gtggccgaca ttctgcgtga cccggaggcc agcgaaaatg ataaatatgt ggcgctgcaa
     8881 ttcctgcgta actccgacat cgcggcgaaa ggcgttctgc caacctgtca ggataccggc
     8941 accgcgatta ttgttggtaa aaaagggcag cgtgtatgga ccggtggtgg tgatgaagcg
     9001 gcgctggcgc gcggtgtcta taacacttat atcgaagata atctgcgcta ctcgcaaaac
     9061 gcgccgctgg atatgtataa agaagtgaat accggcacca atctgccagc gcagatcgat
     9121 ctttatgccg ttgatggcga cgaatacaaa ttcctctgta tcgcaaaagg tggtggttcg
     9181 gcaaacaaga cgtatctcta tcaggaaacc aaagcgttac tgacgccggg gaaactgaaa
     9241 aattacctgg ttgagaagat gcgcacgctg ggtaccgcgg cctgtcctcc gtatcatatt
     9301 gcgttcgtta ttggtggaac ttctgcagaa acgaacctta aaacggtgaa actggcttcc
     9361 gcgaaatact atgatgaact gccaacggaa gggaatgagc acggtcaggc gttccgcgat
     9421 gtggaactgg aaaaagaatt actgatcgaa gcgcaaaatc tcggtctggg tgcgcagttt
     9481 ggtggtaaat acttcgctca cgacatccgc gtgattcgcc tgccacgtca cggcgcatcc
     9541 tgtccggttg gtatgggtgt ttcctgctct gctgaccgta atatcaaagc gaagatcaac
     9601 cgtcagggga tctggatcga aaaactggaa cataatccag gtaaatatat ccctgaagag
     9661 ctgcgtaaag cgggagaagg cgaagcggtg cgcgttgacc ttaaccgtcc gatgaaagag
     9721 atcctcgcac agttgtcgca gtatcccgtt tctacacgct tatcgcttaa cggcacgatt
     9781 atcgttggtc gtgatattgc tcacgccaaa ctgaaagagc ggatggataa cggtgaaggg
     9841 ctgccgcaat acattaaaga tcatccgatt tactacgcgg gtccggccaa aacgccggaa
     9901 ggttatgcct ccggttctct tggcccaacg accgccggac ggatggattc ttatgtcgat
     9961 caactgcaag cgcagggcgg aagtatgatc atgctggcga aaggcaaccg cagccagcag
    10021 gtgacggatg cctgtaaaaa acacggcggc ttctaccttg gcagtatcgg tggtccggcc
    10081 gctgtattgg cgcagggaag tattaagagc ctggaatgtg ttgaatatcc ggaactggga
    10141 atggaagcca tctggaaaat tgaagtggaa gatttcccgg cgtttatcct tgtggatgat
    10201 aaaggaaatg acttcttcca gcagatacaa ctcacacagt gcactcgctg tgtgaaataa
    10261 acagagccgc ccttcggggc ggttttttta catggcacga aagaccaaac atttgttatc
    10321 aaatagtaaa taatgagtga gctaaaagtt gcttaacgaa agcaaaacag aaagaaaaaa
    10381 ttaatcaggt gaggagcagg ccatgaatac agtacgcagc gaaaaagatt cgatgggggc
    10441 gattgatgtc ccggcagata agctgtgggg cgcacaaact caacgctcgc tggagcattt
    10501 ccgcatttcg acggagaaaa tgcccacctc actgattcat gcgctggcgc taaccaagcg
    10561 cgcagcggca aaagttaatg aagatttaga cttgttgtct gaagagaaag cgagcgccat
    10621 tcggcaggcg gcggatgaag tactggcagg acagcatgac gacgaattcc cgctggctat
    10681 ctggcagacc ggctccggca cgcaaagtaa catgaacatg aacgaagtgc tggctaaccg
    10741 ggccagtgaa ttactcggcg gtgtgcgcgg gatggaacgt aaagttcacc ctaacgacga
    10801 cgtgaacaaa agccaaagtt ccaacgatgt ctttccgacg gcgatgcacg ttgcggcgct
    10861 gctggcactg cgcaagcaac tcattccgca gcttaaaacc ctgacacaga cgctgagtga
    10921 aaaatcgcgt gcatttgccg atatcgtcaa aatcggtcga acccacttgc aggacgccac
    10981 gccgctaaca ctagggcagg agatttccgg ctgggtagcg atgctcgagc ataatctcaa
    11041 acatatcgaa tacagcctgc ctcacgtagc ggaactggct cttggcggta cagcggtggg
    11101 tactggacta aatacccatc cggagtatgc gcgtcgcgta gcagatgaac tggcagtcat
    11161 tacctgtgca ccgtttgtta ccgcgccgaa caaatttgaa gcgcttgcga cctgtgatgc
    11221 cctggttcag gcgcacggcg cgttgaaagg gttggctgcg tcactgatga aaatcgccaa
    11281 tgatgtccgc tggctggcct ctggcccgcg ctgcggaatt ggtgaaatct caatcccgga
    11341 aaatgagccg ggcagctcaa tcatgccggg gaaagtgaac ccaacccagt gtgaagcatt
    11401 aaccatgctc tgctgtcagg taatggggaa cgacgtggcg atcaacatgg gtggcgcttc
    11461 cggtaacttt gaactgaacg tcttccgtcc gatggtgatc cataatttcc tgcaatcggt
    11521 gcgcttgctg gcagatggca tggaaagttt taacaaacac tgcgcagtgg gtattgaacc
    11581 gaatcgtgag cgaatcaatc aattactcaa tgaatcgctg atgctggtga ctgcgcttaa
    11641 cacccacatt ggttatgaca aagccgcgga gatcgccaaa aaagcgcata aagaagggct
    11701 gaccttaaaa gctgcggccc ttgcgctggg gtatcttagc gaagccgagt ttgacagctg
    11761 ggtacggcca gaacagatgg tcggcagtat gaaagccggg cgttaatctg caacatacag
    11821 gtgcagccgt ggaatgatca aacgaagcgg ctgcgcctga ggtttataac ggtgctgcac
    11881 attgtcggca tcgtaattta acaactcacc aacgtccggc acgcccgcgc cattatcccg
    11941 attaatcagt gcaatcagtg gtgtagggca ggcgtgttgg acttgttttt gatctccttt
    12001 gtaccagacg cgggcaatcg gctgcacctt cactggacgt ttgattttta acttcgcgtt
    12061 ctgtggcagg gcagcgatat cctgatactc tcgctccagt tttctttgcc actcctcgcg
    12121 cgtccacggt gcgacactgc gtggtgattt caggcttttt tccagctgtg ccaggacttc
    12181 atcacgatgc aaattcttaa tgatatgttt attagcccaa ccaaagcgta aagtggcggg
    12241 gtcgtgcaga acggtgagcg tgcggtaagc attaagggtg atcagccccg gcaaatgacg
    12301 atgcacccat tcaaaacgtg ccgcggtggg gagttctgat tcaaccgtga cgatatgctc
    12361 gaacgtggtc ttgagtttat tgatgtgctg aatatgactg accaacgctg cttgcgaaag
    12421 gttatcgacc tggtaacaca acacgccagg cagacggaca gcggccttgc tgctgcgatt
    12481 ttcggactgt tgttgaataa ataaatggcg gaaatgacgc aacgccagcg actgcgcgtc
    12541 gttgccgaga tgttgtttta cctcaatacg attaagcgga ttatgctcat cctctttttt
    12601 tacctccggc aaagagaaca cgcgggcaac caatagcttg tgttgctcaa gatgagcggc
    12661 aaatgtagcc agctcttgtt ccatctggcg aaaggtagtg ttgagtcggt ctacgagatc
    12721 gtaacgcgcc ataatattaa ccactttagt tacaacatac ttattttata gcacagtcgt
    12781 ggtgacttga ccatcgtgcg cgtagtgtca ggcagaggta aattgcggga tgttatgcca
    12841 taacggccag ctaaacgaga agcgagcacc acccagttcg ctgatgtcac aattaaccgt
    12901 accgcccatt gtcagtgcta ttgagtggac aattgccagc cccagcccgc agccgccggt
    12961 tgagcgatcc cggctgggat cgaggcgaac aaaaggttca aagatatgtt cgcggttttc
    13021 tggggcaatc cctgggccat catcctcaac aattaatgtc gctctattcc ccgacagtag
    13081 caggctggtt tcaaccgttg aatggcagta gcgcagggcg ttattgagca aattatccag
    13141 cacgcgctcc attaagcgca tatccaacgc cgcataatgg ccttgcatga gcgtttttat
    13201 ccgtaccgtt ttatcgggcg ttactgcctg aatatctgcc agatgcgttg acagccacaa
    13261 cggcaggtct ggttcgctaa gatgaagctc gttttgtggg cgatcgagtc gggcataagt
    13321 cagcagctct tcaattaaag cttcaagttg actgatatca cgattcaacg cctgggattc
    13381 ggcggcgctc aggttatcgc tcatctccag tcgataacgc aggcgcacta acggtgttcg
    13441 cagttcgtga gcgataccgt caataagctg ttttttgctg gcaattaagg cgttgatatt
    13501 gtccgccatc tggttaaatg cgataccaag tcgttcaaag ctcgaaccct catcaaagtg
    13561 gatacgttca ttgagatgcc catcgccaaa tcgttgcgcc gctgcttcca gttttaacat
    13621 atcctgccag tgcggacgca tccagataaa caccggaaag gcgagggaaa tagcaataaa
    13681 agcgatcagg gcgatatcca gcaatcgcat ttgatggagg taataaagat aaggaacagg
    13741 accaactgcc agcacgtagt ggctgcgcgg gatacgctgc aaaaacgtgt actgatcgtc
    13801 cagggcgaca atttcgccgc cacgcagtcg gtgcatggaa atatcatcaa gatggtattt
    13861 actcagtggc tcgacacgca gatcgaaaga gagatttaaa tccatctctt tcagcgtctt
    13921 accccagtcg tgtgggggga tctctcgcaa ttcgctgcgc atcagataca gcgaactgtt
    13981 catcaaatca tccagcgact gtttgcccgc gcgttcggcg gtaaatttgt acaccagccc
    14041 aaccagcaga gacatcacaa ggaagcagac aaacaataac aggtaaaact ggataaacaa
    14101 ttttttcatt ggcatcgccc gcaacagaat actattcgta ttaattcacg actgtggagc
    14161 aatgtaaacc gccaactgga taagaccttg tttggaagat aatgtccgga acagacggat
    14221 gtatccccgg tgtattcata ttcagtaaat tcaattttcc accggggctt gtttattatg
    14281 ctaaggttta atttaacgtg acgctttgtt gttctttagt ctctctatcc ttaagaagac
    14341 gggctgatat tacaccggac attattgaac cattcacatt aagcgctgtt cttgccatat
    14401 caacgatcgg ctctatggca ataaaaataa ctactaatgc gacagggaaa ttaagtgttg
    14461 aaaggacaat cagcgccgcg aatgtcccac cactacctac gcctgctacc cctattgagc
    14521 caagcgcgat ggcaggtaaa agagttgcga ggaattgcaa tgagaaggga tcgataccca
    14581 ttgttggagc aatcatcgcc accatcacgg caggataaat cccagcgcag ccattttgtc
    14641 ccatgctgga accaaaagac gctgcaatat tagcaatcgt atttggaacc cccaattttt
    14701 ccaatgcaga aatagccaga ggaattgaag ccgcactact gcgagaaaca aacgcaaagg
    14761 taagtacttg ccagacggtc ttgtaatagt taacggggtt tttacctatt aacagcaaaa
    14821 tgcccgagtg aacaataaac atcacaaaaa cggccagata acaggcgacg atgaacccga
    14881 gcaaattggt aaactgtgaa agctgatacg ttgagaagac tttcgccatt agtgccatta
    14941 ccccataagg cgtcagcgca atgacaatac ggaccatttt cattacccat atttgaatcg
    15001 cgttgatccc cgcgctcaat ttttcgccct ctgctggtgc atctttcttt acttttaaca
    15061 gcgcaatacc agcaagaatt gtaaagatga cgatgccaat aaccgaaaca cttcttgccc
    15121 cggtcagatc aaggaagagg ttggttggga tcaatgacgt aatgagtcgc ggtaaagata
    15181 ccgcagctgt tttcgagacg tcatcgctgg ttaaagagga ttgcatatga ccaaaggcgc
    15241 tggcatcaag gccaattgca ctggcaatag ccaggccaac taaacctgca atcatgacta
    15301 aacacaacat acaggcaaca atagttaaag acatcttgcc aataccagcg ctattttcga
    15361 gcttattaat cgctgaaaga atcgaaatga atatgagtgg aacggcaaca agttttaata
    15421 aattaacata accgttacca gcaatattat accattcgat aacggtttca ctgcttgtct
    15481 caggaatgaa atgcagggag ataccaatga tagcccccag gaaaatcgcg ataaaaacac
    15541 ttttggaaag ggattcgctc ttttgatccg ttttgaaaat cccccatgag cagatcaaaa
    15601 aaagcacaat atatgtgaaa gttgtaaccg ttttttaagc ctcttgcctc attcagcacc
    15661 tgaaatgccg gaaagagttt gttgtaaaaa atctgcgcca taagacgcag ggacgaggcg
    15721 attatagaga aattttttct cattttcata tcaaatgtag ggggtttgga atttgaataa
    15781 gctcttatta attgaattcg ttaattgttc tctttttcag atgaacaaat attagtcatc
    15841 tgattgttta ttgtcactat cccatgcatg aggcgcaaaa agatagcctt tgttacgcac
    15901 agttttaatg cgataaggtt ctgcggcgtt atcgagcagt ttttttctta accgcgaaat
    15961 agccacgtcc acgctacgat ccagtccgtc ataactgacg ccgcgtaaat ttttcagcaa
    16021 tgcatcgcgg tccatgattt gcccggcatg cgtagctagt tcccacaata attcgaaatc
    16081 agctgtcgag agcgagattt cagtgttagc cagggtgact acgcggttga tggggtcgat
    16141 agttaacgtg ccgaaatgca gggctttgta gggagtaaga gacgtttcct gaagaccttt
    16201 ggtcagtgtg gcttgctcat tctgacgcaa atgcaaacgt aaacgcgcta gcaaaacagc
    16261 agggggcgtc gttttgagaa tatagtcgca ggcacccatt tccagtgcca ggatgtggtt
    16321 catatcgcta tcgagagagg ttagaagaac aatcggtcca gaccactttg cgcgtaaatc
    16381 acgacaaatg gtcatgccgt ccttgcctgg tagcatgatg tcgagtaaca ccaaatccgg
    16441 attttctcgc aaaatggttt cttcggcctg gtcgccgcgc ggttctacgg taacctgcat
    16501 atcatgtttt gccaggtacg cggcaatcag tgaaccgact tccgcatcat cttccacaaa
    16561 tacgatagtg ttcataacat tcacggtaga tataaaaacg tcacaataca ccgcgctgtt
    16621 tttacttact atcaatcgtt tctaaacaag ttttcatcag gtattctact gatgacttgt
    16681 tatggaagtg ttaaggtaaa aagatggggc tggtaatcaa agccgcgctc ggtgcgctgg
    16741 tagtgctgtt gattggtgtt ttagcaaaaa cgaaaaatta ttatatcgcc gggctgattc
    16801 cacttttccc gacctttgcg cttatcgcgc attatattgt tgccagcgaa cgtggcattg
    16861 aagccttacg cgcaaccatc atttttagta tgtggtcgat tattccctat tttgtttacc
    16921 tggtgtcgct gtggtatttc accggaatga tgcgcttgcc cgccgctttt gttggttctg
    16981 ttgcgtgttg ggggataagc gcatgggtgt tgattatatg ctggataaag ctgcattaac
    17041 gcagatgacg accgccatca agtgggaaac tgcgtccggt gacaaagcaa ctggtaagta
    17101 agtaatcgac caggtcgatc acttctttct cgccaggcgc ggttttcatc agtgatttat
    17161 tcagcgcctg ttgtcgatat tcggcatcat catgttcatt aaacaggatc agcgatggcg
    17221 caatagaatt cactttcact tccggtgcca gcttgcgggc aaacgagcgg gtcatattat
    17281 ccagtgccgc tttgcttgca gcatacgcaa catgtttgtc gctaccgcgc tccaccacat
    17341 aatcggtaaa gtgaatgata tcgctggcgg cgtgtccgtg cccacgcagt aatctttcca
    17401 gcgcatggtt gagcaggtat ggggtattaa cgtggatctg catcatgcaa gccagtacgt
    17461 cggccagtgg cgcacccggt ttttccgcaa tccacgcact ggcgttatgc aaaatagcac
    17521 gcagaccatg ggtggttttt agtacttcat cggcaaacgc catcacaccg tcgttggtcg
    17581 aaaaatcagc ctgaatacac tgcgcacctg cattaatcag tccatcaatg gctggatagt
    17641 gtgtccgata gctgacaatc accggttgct tttgattaat gaaatgccat gcgagggcga
    17701 ggccgatgcg acgacctccg ccagtaatta atattggcaa gggctgggtt ttacccatcg
    17761 ttatctcctt tgctatccaa cgatgggatg aacgttatcc caccagcatc caggtcgccg
    17821 gaactgaggc aattaacagc atgccaatca gcaccatttc ctgacggttc agtacattat
    17881 catgtgtatg cgttttgcgc gcatacagga aaaccagcag ccccggtgca tacagaacaa
    17941 cggataacag caagtgcatt ggaccagagg cgtacaataa ccataagcca taaatgcagg
    18001 caccgacacc taccgcttta tgcagtggac gggttgcgat tttcagcaag aacgcgccga
    18061 ccaggaaata gggcaccaga atcatttctg aggcgatggt cagcaacgtg ttgtaatcgg
    18121 aaccggtcag ccagatgagt accagacaaa tttgcacgca gatgttggtc aaccacagtg
    18181 aggcggatgg cgcagcttgt gcgttctggc gcgcaaaaat gcgtgggaat gctttatgag
    18241 tggcagccag gaacggaact tctgccgcca tgatggtcca gcttaagtac gcgccgcaaa
    18301 cggaaacgat caaaccggca gcgatgatga tttcgcccca tggtcccatc atttccacca
    18361 tcagaccggc cattgacggg ttacgaattt cagccagttc aggacgtgcc accacaccca
    18421 gcgaaagcag cgttaccagc aagtaaacgc ccagagcgga gagaaccgcc agcagtgtcg
    18481 ctttgccaac atcacgttta ttacgcgcac gcgcagaaac aaccacggca ccttccacac
    18541 caatgaaaac ccacagggtg atcagcatgg tgtttttcac ctgttcccaa acaggtacgc
    18601 caagtgcaag tccggtgaag tcgagcttga aggtatccag tttgaacatc atcatcgcca
    18661 gcacaacaaa cagacccagc ggcaacaatt ttgccagtgt cgccaccagg ttaatgctgg
    18721 cagcggtttg caccccgcgc aggatcaaaa agtgaacaat ccacaataaa gccgatgcac
    18781 cgactatcga ttgccaggta ttaccatcgc caaacaggcg caattccggc gtgtccgtaa
    18841 aaaagcttaa cgcggaaaaa acgatcacca gataggagac gttggcgatg accgcgcaca
    18901 gccagtatcc ccatgcggaa caaaagccga ttagctcgcc aaacccttcg cgggcatagg
    18961 taaagatacc gccatcaagt tcgggacgaa tgcgcgtgag gatcagcatg gcaaaggcca
    19021 gcaataaaat gccagcgcca gtaatacccc agccgatgag cagtgctgcc gggctggcaa
    19081 ctgccgccat attttgcggc agactgaaaa cacccgcgcc cagcattgag cttaatacca
    19141 gcgcggtgag tgcgctcagt cccagtttct tttccatcga tatccagtga ttaaaacgtg
    19201 ataagaagaa taaatattag ggcaaaacgc ataaaaatgg agtttgccac tgaagcgcgg
    19261 gattttacga agagatgtgt ctgcatgcaa tggtcgcggg gaaatttgtg actaaaaatg
    19321 cgaaaggcgg catcaagccg ccttatagga gtaactgaat agttatttat acagatctgc
    19381 gctgacggtc aggttattac cacgtttctg ccactggcgg gtgatgtggt aatacttagc
    19441 acctttcttc gcggcacgtt tcgcaacctg ataggagact tcggtcatgt tgccgtagtt
    19501 gccagagaat ttgatgctgt cgaacgggac catcatcgct gctgtcgctt tgttcagttc
    19561 ttcgacttta gtgccatccg ggagcgtgac ggtgtaacgc ccgccttttg atgactgggt
    19621 ttcaaagaag cgaccgactt cagaacttgg tgatgcggta gtcgcaacac ccgggatctc
    19681 aactttcttc gcggcttcgc caccggcagc cagagctgca cgtcctgctt cggaatctgc
    19741 cgggatcaca tccgggctct ggacgatacg tttcttagca tcttttttat agatgaatgc
    19801 agtaatacgc tggttgccgc cctggttggc atcgatttga cgaacgatgt agaaagagta
    19861 ggcacctttc gcttttgccg ctttggtgat ggcatcattg acttctggct ggctacgata
    19921 gaagccctgg acggtgaccg tgtcaaacgg ttcaatcaga acagcctgat ctttcggcag
    19981 ttcgacaaca ccgttaatta cgcgattact tgtttcttct gctttttcag catcggcttt
    20041 atagaggtca gcgaccacac gccagttacc gctgttacca aaatcagaag tgtcgacaac
    20101 ataaaaagag gcggcacctt ctttatctgc gcgacgagaa acggctttca ccgcttcgcc
    20161 aatagcatta aaacgaccgg taaccactac acggtcaaaa ggtttaaccg ctgccgcttg
    20221 ctccggtgtc agttctgttg ctgcgttaac ggagaatgcc gtagcagaaa gcagtgccga
    20281 cgccaggagg gtgttcttaa gcttcataaa aataatcctt cgccttgcgc aaaccaggta
    20341 ctggtattgt tattaacgag aaacgtggct gattattgca tttaaacggt gtaactgtct
    20401 gcgtcatttt tcatatcaca ttccttaaac caattttaat cctgctcaaa tgaccgtcta
    20461 tgcttaaaaa acagccgtat cagcatcatt actactgaag caactgaatt gtataagtta
    20521 atttaatgtt aagtagtgat tcgtgccggg gcgatgtctc gttttacccg accgtcgaag
    20581 acaattatca gtctttatcc ggcgttctaa ggtgtttatc ccactatcac ggctgaatcg
    20641 ttaatatttt gcgagttcac gccgaaatac tgatttttgg cgctagatca caggcataat
    20701 tttcagtacg ttatagggcg tttgttacta atttatttta acggagtaac atttagctcg
    20761 tacatgagca gcttgtgtgg ctcctgacac aggcaaacca tcatcaataa aaccgatgga
    20821 agggaatatc atgcgaattg gcataccaag agaacggtta accaatgaaa cccgtgttgc
    20881 agcaacgcca aaaacagtgg aacagctgct gaaactgggt tttaccgtcg cggtagagag
    20941 cggcgcgggt caactggcaa gttttgacga taaagcgttt gtgcaagcgg gcgctgaaat
    21001 tgtagaaggg aatagcgtct ggcagtcaga gatcattctg aaggtcaatg cgccgttaga
    21061 tgatgaaatt gcgttactga atcccgggac aacgctggtg agttttatct ggcctgcgca
    21121 gaatccggaa ttaatgcaaa aacttgcgga acgtaacgtg accgtgatgg cgatggactc
    21181 tgtgccgcgt atctcacgcg cacaatcgct ggacgcacta agctcgatgg cgaatatcgc
    21241 cggttatcgc gccattgttg aagcggcaca tgaatttggg cgcttcttta ccgggcaaat
    21301 cactgcggcc gggaaagtgc caccggcaaa agtgatggtg attggtgccg gtgttgcagg
    21361 tttggccgcc attggcgcag caaacagtct cggcgcgatt gtgcgtgcat tcgacacccg
    21421 cccggaagtg aaagaacaag ttcaaagtat gggcgcggaa ttcctcgagc tggattttaa
    21481 agaggaagcg ggcagcggcg atggctatgc caaagtgatg tcggacgcgt tcatcaaagc
    21541 ggaaatggaa ctctttgccg cccaggcaaa agaggtcgat atcattgtca ccaccgcgct
    21601 tattccaggc aaaccagcgc cgaagctaat tacccgtgaa atggttgact ccatgaaggc
    21661 gggcagtgtg attgtcgacc tggcagccca aaacggtggc aactgtgaat acatcgtgcc
    21721 gggtgaaatc ttcagtacgg aaaatggtgt caaagtgatt ggttataccg atcttccggg
    21781 ccgtctgccg acgcaatcct cacagcttta cggtactaac ctcgttaatc tgctgaaact
    21841 gttgtgcaaa gagaaagacg gcaatatcac tgttgatttt gatgatgtgg tgattcgcgg
    21901 cgtgaccgtg atccgtgcgg gcgaaattac ctggccggca ccgccgattc aggtatcagc
    21961 tcagccgcag gcggcacaaa aagcggcacc ggaagtgaaa actgaggaaa aatgtgcctg
    22021 ctcaccgtgg cgtaaatacg cgttgatggc gctggcaatc atcctgttcg gttggctgtc
    22081 aagcgttgcg ccgaaagagt ttcttgggca cttcaccgtt ttcgcgctgg cctgcgttgt
    22141 cggttattac gtggtgtgga atgtatcgca cgcgctgcat acaccgttga tgtcggttac
    22201 caacgcgatt tcagggatta ttgttgtcgg agcactgttg cagattggcc agggaggctg
    22261 ggttagcttc cttagtttta tcgcggtgct tatagccagc attaatattt tcggtggctt
    22321 caccgtgact cagcgcatgc tgaaaatgtt ccgcaaaaat taaggggtaa catatgtctg
    22381 gaggattagt tacagctgca tacattgttg ccgcgatcct gtttatcttc agtctggccg
    22441 gtctttcgaa acatgaaacg tctcgccagg gtaacaactt cggtatcgcc gggatggcga
    22501 ttgcgttaat cgcaaccatt tttggaccgg atacgggtaa tgttggctgg atcttgctgg
    22561 cgatggtcat tggtggggca attggtatcc ggctggcgaa gaaagttgaa atgaccgaaa
    22621 tgccagaact ggtggcgatc ctgcacagct tcgtgggtct ggcggcagtg ctggttggct
    22681 ttaacagtta tctgcatcat gacgcgggaa tggcaccgat tctggtcaat attcacctga
    22741 cggaagtgtt cctcggtatc ttcatcgggg cggtaacgtt cacgggctcg gtggtggcgt
    22801 tcggcaaact gtgtggcaag atttcgtcta aaccgttgat gctgccaaac cgtcacaaaa
    22861 tgaacctggc ggctctggtc gtttccttct tgctgctgat tgtatttgtt cgcacggaca
    22921 gcgtcggcct gcaagtgctg gcattgctga taatgaccgc cattgcgctg gtattcggct
    22981 ggcatttagt cgcctccatc ggtggtgcag atatgccggt ggtggtgtcg atgctgaact
    23041 cgtactccgg ctgggcggct gcggctgcgg gctttatgct cagcaacgac ctgctgattg
    23101 tgaccggtgc gctggtcggt tcttcggggg ctatcctttc ttacattatg tgtaaggcga
    23161 tgaaccgttc ctttatcagc gttattgcgg gtggtttcgg caccgacggc tcttctactg
    23221 gcgatgatca ggaagtgggt gagcaccgcg aaatcaccgc agaagagaca gcggaactgc
    23281 tgaaaaactc ccattcagtg atcattactc cggggtacgg catggcagtc gcgcaggcgc
    23341 aatatcctgt cgctgaaatt accgagaaac tccgcgctcg tggtattaat gtgcgtttcg
    23401 gtatccaccc ggttgcgggg cgtttgcctg gacatatgaa cgtattgctg gctgaagcaa
    23461 aagtaccgta tgacatcgtg ctggaaatgg acgagatcaa cgatgacttt gctgataccg
    23521 ataccgtact ggtgattggt gctaacgata cggttaaccc ggcggcgcag gatgatccga
    23581 agagtccgat tgctggtatg cctgtgctgg aagtgtggaa agcgcagaac gtgattgtct
    23641 ttaaacgttc gatgaacact ggctatgctg gtgtgcaaaa cccgctgttc ttcaaggaaa
    23701 acacccacat gctgtttggt gacgccaaag ccagcgtgga tgcaatcctg aaagctctgt
    23761 aaccctgacg gcctctgcgg aggccgtcac tctttattga gatcgcttaa cagaacggcg
    23821 atgctttgac ctcccgctgt ttgttcaagc gcaattttga caataattgt caacggcacg
    23881 gaaagcagca tacccaccgg tcctaacaac catccccaaa aaatcaacga caaaaatacc
    23941 accaatgtcg aaagccccag cccacgtccc atgatgcgcg gctccagaat attgccgaag
    24001 accagattaa tcagcagata tcccgccagc accagcaacg cttcgtagaa gccattaaac
    24061 accagtacct gagcgatagg ggggattgcc gcgagaactg aaccaatatt agggatgtaa
    24121 ttaagcgcaa aggccagcaa tccccagaca aaagcgaagc gaacatcgag tgcggcgagc
    24181 atcgcccagg cgaccaggcc ggtgatgatg ctgatggctg ttttcagcac cagataatga
    24241 gaaacactgt caatcgcacg ttgaatcgcc gccatccctt caaccggacg cgccatcatt
    24301 tgctgaaatt ttccgggcaa ttgtggcact tcgagcagca taaacagcac cgtcagcagc
    24361 aataaaaata ttgatgacat ggcattagat aactgcgtca ataagttggt gagcaacgtc
    24421 atcgccgcgt tcggatcaat gtaatgcgcc agttggtcaa ctgagacgtc aatccctacg
    24481 cgttgcaaca acggctcaag agcttgcagc ggcgtcataa tagagttgcg atattgcggt
    24541 aacgtccgcg tcaactcgtt gagcgcggaa cccagataag ctaatagcaa caccatcgcc
    24601 atcacgatga ttgtcatcaa aatcgacact gccagtacac gcggcacacg ccagcggacc
    24661 atatgttgca ccagcgggtt aagaataaca gcaataaata atgcgagaat aaacggcacg
    24721 atgatctcgg cggcaaaacg gataccgcag agaataatga ccagcattcc caacataatg
    24781 acgattttta ggccattgag cgtgatgatc ggctttgcca tgcttgtccc cgtttttctt
    24841 cctttgctaa taataatgtc tttttcgctc cgtcatcata acgaaaatca aaccgctcag
    24901 gtaaagaagt gaaaatattt tgagttaatt cttaagctat gatacaaatc aggcgtgttc
    24961 aactaccgag gacaattatc atctgcgatg acgagaagca acactgcgga taattgtaat
    25021 attatggaca atttgttcag gacgttattt tctacattta cccatcttcg tacctcatcc
    25081 actatcttgc tcgtaggtga gcagcactgg cgcaacgcgc tttagtcctt tcttctgcct
    25141 aagcttgccc tatgcgcagg gcttgaccac tttactttgg tttcgctgaa ttaagcgaaa
    25201 attaaaatag ttctcttgca ggagaaggac aatgtatatt tattggattt tattaggtct
    25261 ggctattgct acagaaatta ccggtacgct gtcaatgaaa tgggcgagcg tcagtgaggg
    25321 aaatggcggc tttattttaa tgctggtgat gatttctctg tcgtatatat ttctctcttt
    25381 cgccgttaaa aaaatcgcct taggcgtagc ttatgcgctg tgggaaggta tcggtatttt
    25441 atttattacc ttgtttagcg ttttgttatt cgacgaaagt ttatcgctga tgaaaattgc
    25501 cgggttaacc accctggtcg ccgggatagt gttgatcaaa tcaggtaccc gtaaagcgcg
    25561 taaacctgaa ctggaggtga accatggcgc agtttgaatg ggttcacgcc gcctggctgg
    25621 cattggcaat cgtgctggaa atcgttgcta acgtcttttt gaaattttct gacggctttc
    25681 gtcgcaaaat atttggcttg ctctccctgg cggcggtgct ggcggccttt agtgcgcttt
    25741 ctcaggccgt taaagggatc gacttgtctg tcgtttatgc attgtggggc gggtttggta
    25801 ttgccgccac gttagccgca ggttggatct tgtttggtca acggttaaat cgtaaaggct
    25861 ggattggcct ggtcttgctg ttggctggaa tgatcatggt gaaacttgcc tgatgaagac
    25921 gctgcccgcg ctgtcgggca gcgtttgaac attatttttg cgacagttga tccagcttgt
    25981 cgcgaaaacc ggtaacagaa atggcccggt tatcggcgcg ccagcgatct ttcgcggcag
    26041 gagccgaact ttgcacccca attaattgcc agccgtcatc ggtatgcaac atcagaggcg
    26101 aaccgctgtc acccggcaag gtatcgcact gatgtgacat caccgacgtt tgcgcccagc
    26161 cagtcacttc acagttttga tgactgtata acgtatcgag atgatcttca gggtatcccg
    26221 cctgagtcac tttacgacca gccgatttta atgcggcagt aagcgcggct ttatctccct
    26281 caaataacgg caacggtgta atgccagaag ggggattacg tagcacaatc aatccgaagt
    26341 cccacggtgc agctgcggga ggaacaatcc aaccatcccc atctgctttt aaccgctttc
    26401 ccagtgtcgg atcaacgcgg ccttctatgt cgtggatctc atagcgccaa agacctttat
    26461 ttgacacaaa acgcagcact accgccttat ccgctttacc cttaggcggc gtcaataaac
    26521 agtgtcctgc cgttaatgcc agattgggtg caatcagcgt cgccgtacat aaattgccgc
    26581 tggccgtttc cagttgccca accgcatccc acggtgattg ggtcgtgtca ttcactggca
    26641 cacgatcatc atgaccaaaa aacagggtgc tgacctcatc gtttgccgat ttggcaacgt
    26701 ctggtttatc tgcaaacaca aaagcagacg tcaaactaat tgcacccaac actacagcaa
    26761 tggttgtacg catatcacac tctggtgggt aattatgatt attaaaagca aaccctcata
    26821 aatactatag acgggacggg atgaaagtgg gagtaaaatc agataactac agtcaggaaa
    26881 gataaaaacg ggcggcaatc agtgcgcata aaataaggat gataagaatg aactcaaagc
    26941 gataacggcc caccatacta ccctccgcta aaggcggtgc taaacgttac ccgcttagca
    27001 ccgtaatttt caggcgcgag ggggcgcgcc agcattactg ttgaaaactt acgctgcggg
    27061 ttgtgcagca ggttttgccg gttgctgatg gccgtgtttc ccggcgtgtt tcttcgctgc
    27121 ctgcgctttt tgttcagggg ctttctgttc agctttcgta tttttatgat gctttttagc
    27181 cgcctgcgct ttctgggcag gtgctgcttt atgctgtttt ttatgatgtg tagttttcgc
    27241 tggcgctgct ttggtggtcg tcgcagtcgg agcaggtgtg gtcgcagtct ctgcagcaaa
    27301 ggcggcagaa gacagaccca tagcagcggc aacaaccaga gctaatactt ttttcattgt
    27361 cataccctca atttgttttt tcatttaacc ccactgcggg gccgttgaaa taactatatc
    27421 cctgggaatg gcagacttcc gtgagtggtt ggtttcagcg tgtaacgata tgtacaaacg
    27481 ctgaataaat tacagcgttg ataataacgt attgtgactt aaggaaaatt tagctaccaa
    27541 taatagtagt cttgatcggg ttaactatta tactacttac aaagcggtag aataactgcg
    27601 catcagtaat aaatgacaca cagcaaaatg aatccgttta tttgggtact tataatcctg
    27661 atgacgctag atgcgctgcg ggaattggct ggcgcttcgt ctattttagg atggctatta
    27721 acgttggttt gagcaggcaa taagtccgga cgggtattta ccgcagtccg gacttatttt
    27781 tcaggcgtgc agacgacgat gcaaacgcgt cccgaccagc agagcaatga ccagcatcag
    27841 agcaataaat gcgccgacgc cgttccagcc atagttatgc cagaaaacac cacccagcgt
    27901 cccggcaata ctcgacccca gatagtaact gaacagatac agcgaggagg cctggccttt
    27961 agcgcgtttt gcgcgggggc cgatccagct gctggctact gagtgggctg cgaagaatcc
    28021 ggctgagaag agtaacattc cggcaaagat cagccacagc gagctaaata aggtcatcag
    28081 taaaccaaac ggcataactc ccgtcgaaaa caacatcacc ggaccgcgcc catagcgggt
    28141 ggtcatggtt ccggctttgg gtgagctcca tgtaccggtc aaataagcca gcgataataa
    28201 gccaaccacg gcctgactga catgctaggg ggaaagcatc aaccgatagc cgatgtaatt
    28261 aaacagcgtg acgaacgacc ccatcagcaa aaagccttct gcgaacaata acggtaatcc
    28321 ccggtcacgc cagtgcagac gaaagttgat aaataacgtc ttagggcgca gcgaagtcgg
    28381 gcaaaaatgg cgtgattcag ggaggatttt ccagaacatc aacgccgagg ccagcgcgaa
    28441 acaaccgatt gccgccagag caattcgcca gttgaaaaag tccgtgaaga caccactaat
    28501 taagcgtccg ctcatgccgc caattgagtt gccgctgata tacaacccca tcgaaaaggc
    28561 cacgaaactg gggtggattt cctcgctaag ataagtcatg ccaacagctg ccacgccact
    28621 taacgaaagc ccaatcaagg cgcgcataat caaaatgccg tgccagctgg tcatcattgt
    28681 cgaaagtaac gtacaaatgg aggccaacag tagcgccgtg accatcactg gtttgcgacc
    28741 aatggcatcg gatagcgggc cagtaaatag caaaccgata gccaacatcg ccgtggaaat
    28801 ggacagtgaa atactactgt tcgcgggcgt taagccaaac tcctgcgaaa gcaccggaag
    28861 gataggctgc acacaataga gaagtgcaaa ggttgccagt ccggcagaga acagcgccag
    28921 ggtgacgcgc ataaattgcg gcgtaccgcg tttaataaat tgatttggct gagaaatgct
    28981 ttgcttgtca gtgtcgcttg ccggagcgcc atcaacagtt gtagtacggc tcacttgaaa
    29041 tccttgctaa atatgcctgt ggatcaggct tatacatagg gtaggaaaat cgaattgttc
    29101 tgtctaatat attaataatc tcaaataaga tgttttaaat atgaatattg aacttcgtca
    29161 tctgcgttac tttgttgctg ttgcggaaga gctgcatttt gggcgcgcgg ctgcccgcct
    29221 gaatatttcg caaccgccgc taagtcagca gattcaggca ctggagcaac aaatcggtgc
    29281 ccgactgctg gcacgaacta atcgcagtgt attgctgacg gcagcaggaa aacagtttct
    29341 tgcagatagt cggcaaatcc tgtctatggt ggatgacgct gccgctcgcg ctgaaagact
    29401 gcatcagggt gaagcggggg agttgcgtat tggttttact tcgtccgctc cttttattcg
    29461 ggcggtgtcc gatacgttat cgctgtttcg ccgtgattat cctgatgtcc atttacaaac
    29521 ccgcgaaatg aacactcgcg agcaaatcgc tccgctcatt gaaggaacgc tggatatggg
    29581 attgctgcgt aacacagcgt taccggagac gcttgaacac gcagtcatcg tccatgaacc
    29641 gcttatggcg atgatcccgt acgatcatcc cctggcaaat aatccgagtg taacactggc
    29701 tgaactggct gaactggctg aactggctga actggcgaaa gaaccctttg tcttttttga
    29761 tccgcacgtc gggactgggc tgtatgacga tattctcgga ctgatgcgac gttaccattt
    29821 gacgcccgtc atcactcagg aggtgggcga ggcaatgacc atcatcggtc tggtttccgc
    29881 cggtctgggt gtttcaattt tgcctgcgtc atttaaacgt gttcagctca acgaaatgcg
    29941 ctgggtgccg attgctgaag aggatgcggt ttctgaaatg tggttggtct ggccgaaaca
    30001 tcatgaacaa agtccagctg cgcgtaactt tcgtattcat ctgctgaatg ctctcaggtg
    30061 agggaaattt cagcgaaaaa gcccgaaaaa tgtgctgtta atcacatgcc taagtaaaaa
    30121 tttgacgaca cgtattgaag tgcttcacca tagcctacag attatttcgg agcgcgaaaa
    30181 tatagggagt atgcggtggt tgctgaaaac cagcctgggc acattgatca aataaagcag
    30241 accaacgcgg gcgcggttta tcgcctgatt gatcagcttg gtccagtctc gcgtatcgat
    30301 ctttcccgtc tggcgcagct ggctcctgcc agtatcacta aaattgtccg tgagatgctc
    30361 gaagcacacc tggtgcgaga gctggaaatc aaagaagcgg ggaaccgtgg ccgtccggcg
    30421 gtggggctgg tggttgaaac tgaagcctgg cactatcttt ctctgcgcat tagtcgcggg
    30481 gagattttcc ttgctctgcg cgatctgagc agcaaactgg tggtggaaga gtcgcaggaa
    30541 ctggcgttaa aagatgactt gccattgctg gatcgtatca tttcccatat cgatcagttt
    30601 tttatccgcc accagaaaaa acttgagcgt ctaacttcga ttgccataac cctgccggga
    30661 attattgata cggaaaatgg tattgtacat cgcatgccgt tctacgagga tgtaaaagag
    30721 atgccgctcg gcgaggcgct ggagcagcat accggcgtac cggtttatat tcagcatgat
    30781 atcagcgcat ggacgatggc agaggccttg tttggtgcct cacgcggggc gcgcgatgtg
    30841 attcaggtgg ttatcgatca caacgtgggg gtgggcgtca ttaccgatgg tcatctgcta
    30901 cacgcaggca gcagtagtct cgtggaaata ggccacacac aggtcgaccc atatggtaaa
    30961 cgctgttatt gcgggaacca cggctgcctc gaaaccatcg ccagtgtgga cagtattctt
    31021 gagctggcac agctgcgtct caatcaatcc atgagctcga tgttacatgg gcaaccgtta
    31081 accgtggact cattgtgtca ggcggcattg cgcggcgatc tactggcaaa agacatcatt
    31141 atcggggtgg gcgcgcatgt agggcgcatt cttgccatca tggtgaattt atttaaccca
    31201 caaaaaatac tgattggctc accgttaagt aaagcggcag atatcctctt cccggtcatc
    31261 tcagacagca tccgtcagca ggcccttcct gcgtatagtc agcacattag cgttgagagt
    31321 actcagtttt ctaaccaggg cacgatggca ggcgctgcac tggtaaaaga cgcgatgtat
    31381 aacggttctt tgttgattcg tctgttgcag ggttaacatt ttttaactgt tctaccaaaa
    31441 tttgcgctat ctcaatttgg gccaggaaag cataacttag actttcaagg ttaattattt
    31501 tcctagttta tatttgtgaa gcataacggt ggagttagtg atgctgaagc gtttctttat
    31561 taccggtaca gacacttctg tagggaaaac ggtggtttcc cgcgcattgc tacaagcgtt
    31621 agcctcccag ggaaaaaggg ttgcgggata taaacccgta gcgaagggga gcaaagagac
    31681 acccgaaggg ctgcgtaata aagatgcctt ggtgttgcag agtgtttcaa ccatcgaact
    31741 gccttatgaa gcagttaatc ctatcgcgtt aagcgaagaa gaaagtagcg tggcgcacag
    31801 ttgcccaatc aattacaccc tcatttcaaa cggcctggca aacctgaccg ataaagtcga
    31861 tcatgtcgtg gtagaaggga ctggcggctg gcgcagtctg atgaatgatt tgcgtccact
    31921 ctctgaatgg gtagtgcagg aacaactgcc ggtgttgatg gttgtcggta ttcaggaagg
    31981 ttgcattaac catgcactgc taacagctca ggcgatcgcc aacgacgggc tgccgctcat
    32041 tggctgggtg gctaaccgaa tcaacccagg actggcgcat tatgcggaaa tcattgatgt
    32101 gctgggtaaa aaacttccgg caccgctcat tggtgaactg ccttatctgc cgcgcgccga
    32161 acagcgtgaa ctggggcaat acatccgctt agctatgctg cgcagtgtgc tggcggtaga
    32221 tagagtcacg gtgtaacgtc tgcgaaatta ccgacgcaat tacgcaggca atcaataaac
    32281 cggggagtag ctgatactcc ccggtcattt cacatatcat caacgtcgac ataatcggcg
    32341 cgtgcgtggt tgccgccaac agtgtcgcca tcccggtcaa tccgagtaaa agcgtaattt
    32401 cttcgccatc agggaaccat aatcccaggc tacgaccata caacatgcca atggcaagac
    32461 cgataaatag cgtcggtgta aagacaccac cgggtgcgcc ggaaccgcta ctcgccagca
    32521 cggcacacag tttacaaagg aagatcccgg caatgatcat taacagtggt ggggcggtta
    32581 aaaaggattg tacggtgcta tagccgttgc cccacactgc aggtgtgaac agggaaagca
    32641 gacccacgat caacccgcct agtgccagtt gccagggcgg cgcaagtttg agactcacaa
    32701 atccacgatg acaggcgttc attaacgtta acaacagtgg tccgcacaga cctgccagca
    32761 cacctgtact gataatcagc gcatagtcac gagcctgaac cgtcactgag agttgtacgt
    32821 tgtagagtaa cgcgtcgcta tgattaatca gattgctaac cagccatgcc acgatggcgg
    32881 aaataatcac cgggccgaga gaggccaaca tcatagtgcc aaacagcact tcggctataa
    32941 ataaactgcc agcaagcggg gcacgatagg ccgcagccat tcccgccgcg gccccacagg
    33001 cgatccataa tttccattcc tggcgtggcg taaaacgttg ggcaaaacag gaggcggcaa
    33061 gggcagctaa aagaatcatc gcaccttcgc gaccaattgc actgccgctg gttactacca
    33121 gcagcgaggc aagcgattta accaggcttg ctgcgtaatc gaactgtcca tcggtttgca
    33181 acgcttccat gtaatcggtc ggcgcatgag ggcgttgttg ggtaaatttc tgccagccca
    33241 tcagcaacaa acccgccgcc agtccgccga gcgccggagt tagcagccgt cgccaggagg
    33301 aaaggtttgt cgctgcattg accagactgc cggagtcatt attgaggaac aaccactcca
    33361 gtagcagcat cgcatgacga aacccggcaa cggcaaagac agcgagaata ccgacgactg
    33421 ttgcgataag cagacggcgg aacatagtgc gcagatccgg atagatgtgt agatgatgca
    33481 tgagcagaaa tgggcaatga aacacgggcg actattttgc ggcaaaagag gccaaccgca
    33541 aaagcagtgc gtgctttaag agataaaaaa acgtgacatt gtaacgtatt attgccgaat
    33601 cgcgcgtaat cgtcaccatc cggcaatatt acggtgatcc tatcgaaaga gtggtttaac
    33661 cgcgacagga atcaacagtt gtgattgcca ttgcgccagc gttaagcgag ccagttcacc
    33721 cagtgcacgg tagaaagggt gttctgcttt ttcgataaaa acatcaagaa aacgtgttga
    33781 ccacggaaaa aggtgccatg ccagcaattc ttcgcattct gtctggcgac cattctccgc
    33841 caaccacgca gccatcaaca gcagtgaacc aaaatgatct tccggttcgt tttgcttcat
    33901 ttcaaactga atgcctttct cgcgcatcca ctgacgaagt gccaatgttg aatcgccaaa
    33961 cagcacagat tcgcgatcca gccagaccga cccccacggc ggagacggca gtgcccacgg
    34021 gccgacaaac aaacgctgcc aggcctgggc gtgagtctct tcacactgtg tctgaaaagc
    34081 cgtcaccagc ggcgctaatg acgcttctgg tagaggccac tgagtttccc agccgtcact
    34141 ggtgagtacc gcaaccagag gtgcggcttc cgcactctct ggagcgtaat aaaacagcgc
    34201 gcccaacacg cgcgccgcga cagaaaaatt atcttgctgt gaaaaatggg tcattcctaa
    34261 catcctgaaa tgtgcgggtt gccccgcacc aatttgttaa cctgcaatag ccattcctac
    34321 tgtcatgtgc aggccataaa agagcacacg cccgatcatc tcaccaacaa gtatgaggat
    34381 tagccccaga acaagtccgg caacgtgagg ttcacggcga cgaatgagcg gacaaagcca
    34441 gcatccaaga cctgcgcata acaataccac acgccagacc tgtaaggagg cgtaatctgg
    34501 taccagtgcg ctggcttgtt gcacggatga gtgaatagac gccagagaca aaccttgcag
    34561 cacaatcacc tcggcacaag cgatcagcgc cagaacgcta atgatggcaa atggtgtggt
    34621 attaaatgta acgcgggctg cccgcagaat tgcggcagca agtattggac cgctcaacaa
    34681 taccgtcagg aagaaagcca gcgtagtgta accgttatgc caggttggca cggtgtcgat
    34741 ttgatacacg caggtcatca tccagacgaa aatgacgccg agcgccatgc tgacaagtaa
    34801 ccagagtttc cccaacgctt gcggcatttt accgatgacc gccaccagcc accatagtcc
    34861 gccaacggca aaaaatatgg aaccggcagc aatctcgtta ctcagaccag aagctccgat
    34921 tcgattcagc gagttaaacg ctcgcagtgg cgatcccagg tgcatgacag aggcaataaa
    34981 cccgatgccc atcaacagcc agaggaaaaa catgccacga acaatgtgct gacggtctgc
    35041 gtcatttttg gcggcgaacc agccaattcc gctaacaatc agcgcgccta ctacgcattg
    35101 acccaatacg gtaaagataa ccagtggcca ttcatgccat ccatttccca ttttacactt
    35161 cctccggatt agccagataa ccagtggtat cgccggtcgg gcggctgttg gcgttaggtt
    35221 tgataacgat attgggtttt gtgaaatgcg cgcgcggcag tggcgcgacg gcagccagag
    35281 tgccgtgctt ctggcgcagc tcttcaattg gaccgaactc aagtgcacgc agcgggcagg
    35341 attcgacgca tatgggttgt ttcccctcgg cgacgcgcga ataacaacca tcgcacttcg
    35401 tcatgtgccc tttttcagca ttgtactgtg gtgcgccgta cggacaagcc atgtggcagt
    35461 agcggcagcc gatacaaaca tcttcatcaa ccaccacaaa gccatcttca cgcttatgca
    35521 ttgcgccgct ggggcagact tttgtacatg cggggtcatc gcaatggtta caggagatgg
    35581 agagataata agcgaacacg ttctggtgcc agatgccatt atcttcctgc cagtcgccgc
    35641 cagcatattc ataaatacgg cggaaactga cttccgggcc aagatcttta aaatctttgc
    35701 acgccagttc gcaggtttta cagccagtac agcggctgga atcgataaaa aatccatatt
    35761 gtgtggtcat gggctactcc ttaaaccttt tcgatctgga caagattgct gtgcgacggg
    35821 tttccctttg ccagcggtga agggcggtga gaggtcagaa tattgatact gccgccgtga
    35881 tcgacccggt caccaaacat atccgcttta agccacgcac cttgcccgat ggcggtaacg
    35941 ccaggcagaa tacgcggagt cacttttgcg gcaatcagca tttctccatt attgttaaat
    36001 acccgcacgg tatcgccatg acggataccg cgtgcctgag catcaatggg gttgatccac
    36061 acctcttgtg ggcaggcctg ctgtaacaca tcaatattgc cgtagctgga gtgggtacgc
    36121 gctttggttt ggcgacttca ctcagcatcc agtagatggg tttgcgttca aattttgctg
    36181 aggttgcggg ttgggcgagg atcacatagc ccatattccc tgcagattca tgagagataa
    36241 ggtcttcttg ctctgttggc atcaggtcag gcaacaggat atcgcaatac ttagccgagg
    36301 ccgtcatgaa gtggtcaatg ccaacaatca tctcgcactt gctgtcatcc tgaagcacct
    36361 catgggtgtg attgatgtcg ccatgttgat tgatcaatgt gttactggcg tagcaccata
    36421 aaaacttgat ggggacatcc agtttttctt ttccacgaac accatcacgg gtcgcggtca
    36481 ttttcgtacc atggtcgatg gcatctgtcc atgtaaaaac ggaaatctgc gttttaacag
    36541 gattctcgag catcgggaac cattctaccc ccagatccca gctaccttcg cgtacacctg
    36601 agttgccgcc gtttatgccg acgttaccgg taagaacgga aagcatggca atagcgcggg
    36661 acgtttgttc gccgttggag tgtcgttgtg gcccccaacc ctgacaaata taagcaggtt
    36721 ttgctgaacc gatctctcgt gccaactgga taattttttc tgccgggatg ctggtgattt
    36781 ttgctgccca ttccggcgtt ttagctatgc cgtcaggccc ttcgcccaga atataggctt
    36841 tataatgcgc gttacgtggt gcgttggcgg gcagcgtttt ttcatcgtaa ccaacacaat
    36901 atttgtcgag aaatggctga tcgaccatgt tttcagtaat cagtacccag gcaatcgcac
    36961 aggccagtgc gccatcggtg ccagggcgaa tgggcagcca ttcatcttca cgcccggcag
    37021 ctgtgtcgtt atagcgtgga tcgatgacga tcatgcgtgc gtttgaacgt tcgcgggctt
    37081 gctcgacgta gtaagtgaca ccaccgccgc tcatccgcgt ttctgccggg ttatttccga
    37141 acataacgac cagtttcgta ttggcgatat catccgggct gttgccatca ttggcaccga
    37201 acatataact cattgcggca ctgatctgtg cggtactgta gctgccatag cgactgagaa
    37261 aacccccgca agagttcatc agacggtacg ggacgttcga gttggtgata tttccgccat
    37321 ctacgcctgt tccgtacagg acatgtacag cctcattgcc gtaatctttc aggatccgcc
    37381 gaagattatc actgatggta tccagggctt cgtcccaact tatccgttca aatttacctt
    37441 caccgcgctc gccgacgcgc ttcatgggat atttcaacct gtcaggatga ttcatccgtc
    37501 ggcggataga gcgcccgcgt aaacatgccc gaacctgatg attaccgtaa acgtcgtcac
    37561 ctgtcgtatc agactccacc cagtacacgg tgtcatcttt cacatgcaaa cgtagcagac
    37621 agcggctccc gcagttaacg gtgcaggaac tccagaccgt tttctcttct accggagcct
    37681 ctgccgcccg gaccatttgg gaaaatggca gagtgaaagc actgcttgcc agcgcgagac
    37741 tgccaagtgc ggaggttttc atcagacttc tatggctgat ttcagccttc atgagcgcct
    37801 ctgtggtatg gattttcatc attactcact tattgctttt caaacaaaat gtcatgccag
    37861 aatttatggt tgtagtgggt tatatttttt cgatctcgac cagattagtg tgctgcgggt
    37921 ttcccttcgc cagtggtgaa gggcgcagag tggttagcgt attcacacat ccgccatggt
    37981 cgattttatc gccagacata ttgggctcgt gccaggctcc ctggcccata gcgctaactc
    38041 cagggagaat gcgtggtgtt actttggctg gtagccgaac ttcgccacga tggttaaaca
    38101 cccgcaccat atcgccgttg gcaatcccac gtttctgtgc atctataggg ttgatccaca
    38161 cctcctgacg gcaggctgcc ttcaggacat caatgttgcc gtaggtcgag tgagtacggg
    38221 atttgtaatg gaaaccaaac agttgcagtg ggaaggttct acgttcaggg gagtcccagc
    38281 cttcaaaggt tgaggcataa actggcagtg ggcttatcac ttcatctttt tccagttccc
    38341 aggtacgggc aatttccgcc agcttgctgg aataaatttc aatcttaccg gaaggcgttt
    38401 taagtggatt tgcctcgggg tcgtcacgaa atgctttgta ggcgacaaaa tggccattgg
    38461 gatctttacg cttatagata cccatttttt tcagttcgtc gtaagacggt aacgccggat
    38521 ctttggcaag cattttggcg tacagatgtt gtaaccattg ttcctgcgtg cgaccttctg
    38581 tgaacttttg atagacgtca ggtccaagac gtttcgcgac ttcactcagg atccagtaaa
    38641 tcggtttacg ttcgaatttt tcgctggtga ctggctggag gaaaatgaga tatcccatgt
    38701 taccggcgta gtcgttagga ataatatctt cctgctcaac ggtcatcagg tcttccagca
    38761 gaatgtcggc atattttgcc gatgaggtca taaagttttc gatgaccaca atcatttcgc
    38821 atttcgattc gtcctgcaga atttcatgcg ttttgttgat gtcagaatgc tgattaacga
    38881 gggtatttcc cgcgtagttc cagatggact ccactgtcag acggatggac ggacttgaag
    38941 tagttgactc aacgctactt aaggtcctga tatgactgac attaaccatg acaacactgc
    39001 atatccatcc agttatttct ctgggtctcc ctatctgtat gggcaagtct acaccccaag
    39061 tcctgacaca aggctgacac aaatcaatct actcggcgta gctaagcctc agttagtccg
    39121 tagagccaat ggtcgatata ctatccgctt cagactcaag ggacagacca caccgtttct
    39181 ttcagtatcg acccgcagca cagacaggag agtagccaca atgcgtcaga gagagcttgc
    39241 agccacagct aaggccttca tgttggatag acctgaagtc tcactacagg agcttacaga
    39301 gcatctccgg tctatggctg agcagttcct gacagacgct agtgacgatt actggaatgg
    39361 acttgaggtc gctactctgg tcgacgagaa gtccaacctg aaggagctag ccgctacgca
    39421 agccttgagt ctggaccagc agaagggcat aaggttagcg ctggaagtcc tgaccgcagc
    39481 acagcagaga gtcgacactg gtgatacctc tggtctgatt aagctgattg atgataataa
    39541 ccatactgat tactcaacta taggcgactc tacgtcaatt ctgaagaatg agcaggggga
    39601 tagaccagta gttttcacac aggagcgcca acctgaggtc actatctgta gtttttctga
    39661 gctagtgtcc tcgctgctgg ctgagaaggt ccagaccctg aagtcctcca gctacaagga
    39721 cctctcgtcc tcactaaata ctgtctcccg gttcctgcct gaagacatgg acctgatgtc
    39781 tcgctctgag tggctggctg tcagggacgc tatgctggct tctgaggtga gaccgtcgac
    39841 gattaacaag ctgctgacta aggcgaagat gtgtctggac tatggcctga tgaatgggca
    39901 acttgagggc cgcaacccga ttgagcgcat gaagttaact aaggatgtag actccaagcg
    39961 tagagcattc actgacgagg agatggagag acttctggtc cgtgttgagt ctgagtatca
    40021 gttcacacga cacacggctc acacgacctc tgaggcccgt agatgggcgt ctctggtgtc
    40081 tgtagttact ggcgctcggt ctgctgaggt ctgccacctg actaaacgtg acattgtaac
    40141 cttggacaat gggctggtct gtatcgacat caatgaagat ggggacggta agtctgtgaa
    40201 gaacaagcat tcagtccgac ttgtgccgtt gaccgatggg gcctgtggtc ttgacctgaa
    40261 gtccttcctc gaatgggtag acatgcagcc tgacgagggt cctctcttcg ggatgactcc
    40321 aagcgcatac tctagctggt ttaactcaag ggtcctgact gaagctctgg gagacgctac
    40381 ggatgtctct ctccacagtc tgaggcactg gctagcgacc agaatgaaag agcgtggagc
    40441 caatctggtg gacgctcagg gcatcttagg tcacagcagt cagtccatta cgtatgacct
    40501 gtacgggaaa ggacacgctg ttggtaggct ggctgaggtg ctgaagacag ctttacttta
    40561 agtaggaaga cccaaggtgg gagaccatat acggcttgat ggttgagacc ttgggtcact
    40621 gaagtctgac tgaagttagg gagggtgata ataatcataa tccctcctga ctataggccc
    40681 actggttcgc cttcggctgt ccgactctaa gtctgtctgt ggtcttattt aaactggttc
    40741 cctaactcag gtcctccaac ttggtgtctt acttaaactg gttccctaac tcaggcccct
    40801 ccaaatttcg ttgtctgtca gcgacttaaa tatcccctca ccttagaaga gtttagggtt
    40861 cagaccctta actatcacct taggagagac gggccgtcct ccacccaatc aaaaccttaa
    40921 gaggaggacc atacatggct attaaaattc agtgctacac cgagtccgac aatgcggctt
    40981 tcttcggtga cgattccgac caccagcacc agctaccagc accgacatat gacgaaccta
    41041 aggcccgcac aggtaa
//
LOCUS       DAETQL010000031        40545 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.031, whole genome shotgun sequence.
ACCESSION   DAETQL010000031 DAETQL010000000
VERSION     DAETQL010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.031"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..956)
                     /locus_tag="K0W65_001750"
     CDS             complement(<1..956)
                     /locus_tag="K0W65_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBI3095041.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1202..1828
                     /gene="gstB"
                     /locus_tag="K0W65_001751"
     CDS             1202..1828
                     /gene="gstB"
                     /locus_tag="K0W65_001751"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HBI3095042.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="K0W65_001752"
                     /pseudo
     CDS             complement(1825..2939)
                     /gene="yliI"
                     /locus_tag="K0W65_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="K0W65_001753"
     CDS             complement(3050..3433)
                     /gene="bssR"
                     /locus_tag="K0W65_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBI3095043.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3646..4971
                     /gene="rimO"
                     /locus_tag="K0W65_001754"
     CDS             3646..4971
                     /gene="rimO"
                     /locus_tag="K0W65_001754"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBI3095044.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5018..6346)
                     /locus_tag="K0W65_001755"
     CDS             complement(5018..6346)
                     /locus_tag="K0W65_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBI3095045.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6354..8696)
                     /locus_tag="K0W65_001756"
                     /pseudo
     CDS             complement(6354..8696)
                     /locus_tag="K0W65_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="K0W65_001757"
     CDS             complement(8874..9785)
                     /gene="gsiD"
                     /locus_tag="K0W65_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBI3095046.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="K0W65_001758"
     CDS             complement(9788..10708)
                     /gene="gsiC"
                     /locus_tag="K0W65_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBI3095047.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="K0W65_001759"
     CDS             complement(10726..12264)
                     /gene="gsiB"
                     /locus_tag="K0W65_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBI3095048.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="K0W65_001760"
     CDS             complement(12284..14155)
                     /gene="gsiA"
                     /locus_tag="K0W65_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBI3095049.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="K0W65_001761"
     CDS             complement(14142..15107)
                     /gene="iaaA"
                     /locus_tag="K0W65_001761"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBI3095050.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15311..16546
                     /gene="moeA"
                     /locus_tag="K0W65_001762"
     CDS             15311..16546
                     /gene="moeA"
                     /locus_tag="K0W65_001762"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBI3095051.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16546..17295
                     /gene="moeB"
                     /locus_tag="K0W65_001763"
     CDS             16546..17295
                     /gene="moeB"
                     /locus_tag="K0W65_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBI3095052.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="K0W65_001764"
     CDS             complement(17474..18136)
                     /gene="fsa"
                     /locus_tag="K0W65_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBI3095053.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18267..19166
                     /locus_tag="K0W65_001765"
     CDS             18267..19166
                     /locus_tag="K0W65_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBI3095054.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19172..21604
                     /locus_tag="K0W65_001766"
     CDS             19172..21604
                     /locus_tag="K0W65_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBI3095055.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21750..22565
                     /gene="ybiV"
                     /locus_tag="K0W65_001767"
     CDS             21750..22565
                     /gene="ybiV"
                     /locus_tag="K0W65_001767"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBI3095056.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22717..23982
                     /locus_tag="K0W65_001768"
     CDS             22717..23982
                     /locus_tag="K0W65_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HBI3095057.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRLLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24223..25815)
                     /locus_tag="K0W65_001769"
     CDS             complement(24223..25815)
                     /locus_tag="K0W65_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBI3095058.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26034..26954
                     /gene="ldtB"
                     /locus_tag="K0W65_001770"
     CDS             26034..26954
                     /gene="ldtB"
                     /locus_tag="K0W65_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBI3095059.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27012..28129)
                     /locus_tag="K0W65_001771"
                     /pseudo
     CDS             complement(27012..28129)
                     /locus_tag="K0W65_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
     gene            complement(28126..28593)
                     /gene="mntR"
                     /locus_tag="K0W65_001772"
     CDS             complement(28126..28593)
                     /gene="mntR"
                     /locus_tag="K0W65_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HBI3095060.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28779..28907
                     /gene="mntS"
                     /locus_tag="K0W65_001773"
     CDS             28779..28907
                     /gene="mntS"
                     /locus_tag="K0W65_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBI3095061.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29179..30762
                     /gene="opgE"
                     /locus_tag="K0W65_001774"
     CDS             29179..30762
                     /gene="opgE"
                     /locus_tag="K0W65_001774"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBI3095062.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30810..31325)
                     /gene="ompX"
                     /locus_tag="K0W65_001775"
     CDS             complement(30810..31325)
                     /gene="ompX"
                     /locus_tag="K0W65_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBI3095063.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31378..31443)
                     /locus_tag="K0W65_001776"
     CDS             complement(31378..31443)
                     /locus_tag="K0W65_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095064.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31678..32565
                     /gene="rhtA"
                     /locus_tag="K0W65_001777"
     CDS             31678..32565
                     /gene="rhtA"
                     /locus_tag="K0W65_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBI3095065.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32864..33367
                     /gene="dps"
                     /locus_tag="K0W65_001778"
                     /gene_synonym="pexB"
     CDS             32864..33367
                     /gene="dps"
                     /locus_tag="K0W65_001778"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBI3095066.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33771..34517
                     /gene="glnH"
                     /locus_tag="K0W65_001779"
     CDS             33771..34517
                     /gene="glnH"
                     /locus_tag="K0W65_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBI3095067.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34656..35315
                     /gene="glnP"
                     /locus_tag="K0W65_001780"
     CDS             34656..35315
                     /gene="glnP"
                     /locus_tag="K0W65_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBI3095068.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35312..36034
                     /gene="glnQ"
                     /locus_tag="K0W65_001781"
     CDS             35312..36034
                     /gene="glnQ"
                     /locus_tag="K0W65_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBI3095069.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36295..38519
                     /gene="ybiO"
                     /locus_tag="K0W65_001782"
                     /pseudo
     CDS             36295..38519
                     /gene="ybiO"
                     /locus_tag="K0W65_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38516..39442)
                     /gene="rlmF"
                     /locus_tag="K0W65_001783"
     CDS             complement(38516..39442)
                     /gene="rlmF"
                     /locus_tag="K0W65_001783"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBI3095070.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39718..39978
                     /locus_tag="K0W65_001784"
     CDS             39718..39978
                     /locus_tag="K0W65_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBI3095071.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40243..>40545
                     /locus_tag="K0W65_001785"
     CDS             40243..>40545
                     /locus_tag="K0W65_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBI3095072.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT        10095 a        10720 c         9867 g         9863 t
ORIGIN      
        1 gagcccgctt cgcctgcccc gagattcact tcgatcttat caccaaacca gacgcgctga
       61 gtcacaaagg tggcatcagg tttaattggc gtcacagttt caaagaagcg gaaaccccag
      121 gtcaataatt tctcagactc attaaaacgg atacggtcag ttttcgcccc cagcactacg
      181 gagattaaac gcatatcacc ctgggtagcc gaagcaacca gattatatcc cgcacctgcc
      241 gtggttcctg tcttcatgcc atcaacattc agattgctgc tccacagcag acggttacgg
      301 ttaggctgac gaattttgtt gaaggtgaac tctttctctt tatgaatggc gtactcttcc
      361 ggcacatcgt ggatcaatgc tttacccagc aatgccatat cgcgcgcggt actgaactgc
      421 cccggcgcat ccaggccgtg caccgtctgg aaggtagtgt tggtcagccc cagttttttg
      481 gcataaccat tcatcagacc aataaaggac tcctggctcc cggcaacgta atctgccagc
      541 gcaatacagg cgtcattacc ggactggata atcacacctt tgttcaagtc tgccaccgaa
      601 acctgatcgc ccggtttgag gaacattacc gatgaaccac gcagtgccgg atttcccgtc
      661 gcccaggcat ctttaccgac cgtcaccata tcggtgagtt taatcttatc ggccttaagc
      721 gcctgcccaa ccacatagct ggtcatgatt ttagtcaggc tcgcaggatc cagtttctca
      781 tccgcattgc cttctgccag cactttaccg ctggcgtaat ccattaaaat ccatgcacgc
      841 gcatccacgc tcggcgcttc aacggtttgt tcggccgcga atgccgttgg ggcaaaaagg
      901 aataaaaatg cagaacccgc tgcaagacca cgaaggagag aggagtattg cgtcataaat
      961 gccacccgag tatccattca aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt
     1021 aataacgcac acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca
     1081 atatcctgcc gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta
     1141 actttacttc accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag
     1201 tatgattacg ctgtggggtc ggaataattc aactaacgta aaaaaagtat tgctgacgct
     1261 cgaagaactg gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca
     1321 cgatgctgat tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga
     1381 aagtgatctc attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg
     1441 gcaaaaacgc ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga
     1501 ctgggcaaac cagacgctca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac
     1561 accaccggaa gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct
     1621 gtttgccctg ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg
     1681 tgtgggcgat atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg
     1741 gacaccgcgt ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg
     1801 caaagtggtg atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact
     1861 ggactcgtcg gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg
     1921 ctgccctctg tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat
     1981 cacaatgaca tctttatctt tcagctccca ataaataatt tttgctgcca ctgggggaat
     2041 ttatcgctgt tatagaaggc catgccgctc acagcgggcg aatctttcca gtaaaaaaca
     2101 ggttgctcgg tcccggcgac gatctcccct ttcgcttccg gtatcttaaa gcctgaatag
     2161 ttgattcccc aggttgccag cggccagccg tagtttttgc ctttttgcgg gatattaatt
     2221 tcatcaccac cgcgcgggcc atgttcattc agccacagtg cattactcca cggattcatc
     2281 gccattcctt gcggattacg aatgccataa gaccagatct cggcgcgcgc accggattcc
     2341 tttataaaag gattatcatc cgggatttcg ccctggtcgg tcagacgcac cagtttgccc
     2401 tgtaatttat ccagatcctg cgccgtcggg cgctgattgt tttcgcccag agcaataaaa
     2461 agataacctt taccgtcgaa taccagccgc ccgccaaaat ggttgccggt agacagtttt
     2521 ggcatctggc ggaagacggt gcggaagtcg gtcacttttg agagatcatc acttaagcgg
     2581 ccataaccca cagcagttcc ggctttgcca tcatcgccaa cttcggaata acttaaccag
     2641 atgcggcgag actgagcaaa atcaggcgct aaaaccacgt ccagcaggcc gccctgcccg
     2701 tgcgcccaaa cgtccggaac tccggaaagc ggcgcagata atccttttcc tgcttgccag
     2761 tgacgcaact cgccgccgcg cagagtgatt aacataccgt gattatcggg taaaaaggcc
     2821 agtgcccagg gatggtcgag tttgtcttgc agtacttcga catttaccgt tgcaggagcc
     2881 gcccagagag cggaagaaag acaaataagg ggcacaagga aaaaggattg tcgatgcata
     2941 acgcctccgg attgactctg gcttaaagcg tagtcagtgg aggagataac aaattcattt
     3001 ttacaaaaac ttaaacatga agggggagac gctttctccc ccttagtttt caggccttct
     3061 caagcatggc gtgcttctgc aggctctgga tactcagcgt taagctcatc agacaatttt
     3121 caagcttatc ggcgttgacg gtaataacag tcgggcaatc atggtgccca ctcatcaaac
     3181 atactgcggc tgtcgctaat gcttcttcag catgatgaag agcactccac tcttcctgat
     3241 ccagatgaag attcaaccgc agcgatttat cgtgcagttc gcgattcagt ttaaaaaagt
     3301 tatctcgtag atgattgctt tcgctgacgg acatgtatcc ttttgccttc ctcagttgca
     3361 cgtatgcagc gaggtcaacg cgtgcgtcga tcaaccggtt cagcagatcg attcgctgtc
     3421 tgtcaacgaa catacttcga tcctcctctt tccagccttg ttgcaattaa atagtatggc
     3481 tattttttga gcaaaatgct gtccaggatc aattcttcac gccgaattaa caaaatttac
     3541 atttccttcg cctccgccag cgaatgagat tgcgatacaa attttatcca gaactctgta
     3601 gaatccctgc cctgaccttt cctgtaactg aatatttttt aagctatgag caaagtaact
     3661 ccccagccga aaatcggctt tgtttccctt ggctgtccga aaaaccttgt cgattcagag
     3721 cgtattctca ccgaactccg cactgaaggt tatgacgtgg taccgagcta tgacgatgcg
     3781 aacatggtga tcgtcaacac ctgcggcttt attgacagcg cggtacaaga atcactggaa
     3841 gccattggtg aagcgttgaa tgaaaacggc aaggtaattg tgaccggttg tctgggggca
     3901 aaagaagatc agatccgcga agtccacccg aaagtgctgg aaatcaccgg gcctcatagc
     3961 tatgagcagg ttctggagca cgttcatcac tacgtgccaa aaccgaaaca caacccattc
     4021 ctgagcctgg tgccagaaca aggtgtgaaa ctgacgccgc gtcattatgc ctatctgaaa
     4081 atttcggaag gctgtaatca ccgctgcacc ttctgcatta ttccgtctat gcgcggcgac
     4141 ctgatgagcc gtccgattgg cgaagtgtta agtgaagcga aacgtctggt agatgcgggc
     4201 gttaaagaga ttctggtgat ctcgcaggat acttccgcct atggcgttga tgttaaacat
     4261 cgtactggct tccacaacgg cgagccggta aaaaccagca tggtcagcct gtgcgaacag
     4321 ttatcgaaac tggggatctg gacacgtctg cactacgttt acccttatcc gcatgtggac
     4381 gacgtcatcc cactgatggc agaaggcaaa atcctgccgt atctggacat tccgttgcag
     4441 cacgccagcc cgcgcattct caaactgatg aagcgtccgg gttctgtaga tcgccaactg
     4501 gcgcgcatca aacagtggcg cgaaatctgc ccggaactga ccctacgctc aacctttatt
     4561 gtcggcttcc ctggcgagac ggaagaagat ttccagatgc tactcgactt cctgaaagaa
     4621 gcacgtctgg atcgcgttgg ctgctttaaa tacagcccgg ttgaaggtgc agacgccaat
     4681 gccctgcctg accaggttcc ggaagaagtg aaagaagaac gctggaaccg tttcatgcag
     4741 ctgcagcagc agatttccgc cgagcgcctg caagagaaag tgggccgtga aattctggtg
     4801 attatcgacg aagtggacga agaaggcgcg attggtcgca gcatggcaga tgcaccggaa
     4861 atcgacggcg cggtttatct caatggtgaa accaacgtta agccgggtga tatcctgcgt
     4921 gtgaaagtcg agcacgccga tgagtacgat ttgtggggta gccgggttta attaattcag
     4981 gccgggcaaa atcatttgtc cggcctgcga ataatgatta ctgccagcta atctgtgccc
     5041 tgccgttttt cttactctta tacagccgct catccgccag cattttcaag cgttcgacgg
     5101 tagtacattc atttgaatgc gctacaccga tactggtgct taaattaatg cttcccacct
     5161 ggaaatgaac gatttctttt agtatcgata gcaactcttc cagcgtgccg ccagtataaa
     5221 tcaccgcaaa ttcgtcacct ccgaagcggt agactttacc tttatctacc gggatctgtg
     5281 atttaaggat tttcaccact ttcatcaaca cttcatcgcc cagcaaatga ccgaaggtgt
     5341 cgttaatgtt tttgaattta tcgacgtcaa acagacacaa ataaccgcct ttttccggca
     5401 gcgcttttaa atcttcatca aaggattttc tgcccattgc tttggtcagg tcgtccagga
     5461 acgccgagtt atacatactg tagagttttt tgacatcggc agccgaaaag aaaagcccgc
     5521 catccagtac gcgttcaaaa tggttattaa tgatggcttc tttctccaca ataccacgat
     5581 taatcactct gcgtaccaga tagaggcaac tggcacccaa tatcactgcc accaatgcgg
     5641 caaagaaaat aatcaatgta caaacccgca aataaacatc ttttttatac gcaaatacgt
     5701 acaatccggt cggcaaatag gcgaagtgct ccatcgcttc cgaaacggtg aagatggcgt
     5761 ttttagcatt atattcatca ccggtcaccg gtttaccgtt cttaaataga gagaacatca
     5821 ggttctcgct gtccatcacc agcgattttg acagtaaaga aaaacgctcc agaccaagca
     5881 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
     5941 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
     6001 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
     6061 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
     6121 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
     6181 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
     6241 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
     6301 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
     6361 tttactaatt ttccactttt atcccaagcg gagaatggca tgggtttctg gtacagatac
     6421 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
     6481 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
     6541 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
     6601 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
     6661 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
     6721 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
     6781 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
     6841 tgcagtaaga aatcatcctt cagcgacgca cgttgactaa aatgtctgaa ttaacccatt
     6901 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
     6961 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
     7021 gcgctcagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
     7081 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
     7141 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
     7201 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
     7261 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
     7321 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
     7381 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
     7441 ctgacgattt tattgcgttc atcaacacca aacaccgcct ccacggcttc cagaccacac
     7501 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
     7561 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
     7621 atttcggcat ctttcatttc cgcgatcgaa tttttgatgc tgataatctc tttcgcatcg
     7681 ccttcgcgaa ttttcagagc ctcgatctga tccgttgagt cgggcaaaaa cgccagcgta
     7741 ttaaccagct cggtcatttc agaaaattgc cgacggaaca cacgagtggt catgaaatat
     7801 accaccagca acacgataat aatgaacgca aacggtagat aaaaaagttt atctgatgcg
     7861 ttcaataaat taccgccagg ataataaaaa tagatttcga acaatgcatt atttggcact
     7921 gctgatgtct ttgcaatata ctccgtatcg acagaatcaa taatattata attaccttcg
     7981 ctattataaa taatcagttt ttcacgagcg agttgattct tggcaatgat ctcatgacct
     8041 tgtttcaaaa atagaacttc atcattagcg ctgatttgat caagatactc gttcagatac
     8101 cgccagttta aatcgacatg aattttatct accgctaaca aatcgtgatc tttgccgttg
     8161 atacgtagcg taaaagagat acatttttta cccggacgat caaagcaatt gtatgccgga
     8221 ttccagctgt tgttagagtc atctgaagcc accgaccacg gtcgtttcga tgggttaaaa
     8281 tcggcaaaaa taacccgccc tgactcgtcg acaagcggtc catttacttg ttcctgatga
     8341 taaacaacga ttttatcatt cgccctccgc gaaaagagat aatatttatt atcatcaagt
     8401 accagtccca ccgagtctaa aaatggcatc atttccattt tcagaatcac ggcgcgttga
     8461 atctcctctg tattttttaa taaatacagt gagtccttta atgtcagggc cgaacgttca
     8521 atatctttaa agaaatagcc aacgtttttc tgaataagtt caaagcggct atcggcacgt
     8581 tttaccgcgc gttccgatac cagttgatag ttaatcatga aaaaaagacc aataaatgac
     8641 agtgctgcca ataaaaataa aattatcagc cttatctttt cgtataaact cagcatgtta
     8701 atacatttaa caaaataact atctgaacgc tgtgccgctg caaaaatagt cccttgctgc
     8761 ggctttttaa ccaaattacc cgattctaca aaatcagcag aaagttacaa cacttgctta
     8821 gggcaataat ttcctgtgag gtgattaccc tttcaagcaa tattcaaacg taactatcct
     8881 ttaattttcg gatccagcgc atcgcgtaaa ccatcgccca acaaattgaa cgccagtacg
     8941 gtcagaaaaa tagccagggc cggaaaaaca gcgacatgcg gcgcgataac catatccgct
     9001 cgtgcctcat tgagcattgc tccccactct ggtgtcggcg gctgcgcacc gaggccgaga
     9061 aatgacaggc tggcggcaga gataatcgag gtgccaatgc gcatggtgaa aaacaccacg
     9121 atagaagaga cggtcccagg caggatatga cgcaataaaa tggtcatatc gctggcacca
     9181 atactgcgtg ctgactcaat aaaggtttgc tgtttcaaca ccagcgtgtt gccgcgcacc
     9241 aggcgggcaa acgcggggat ggaaaaaatg gcgactgcga taatcacgtt agcaatgccg
     9301 cttcccaaca ccgcaacaac agcgatcgcc agtaaaatac ccgggaaggc aaacagcaca
     9361 tcgcaaatgc gcatgatcag ccgatcccac cagccttcat aatatccagc caacaagccc
     9421 agcaacgtcc ctatcgccac accgataaac acggcaaaca cgcccgccgc cagcgagatt
     9481 tgcgcaccaa ccaggacacg gctgaaaatg tcgcgcccca gtgaatcgac gccaaaccag
     9541 tgctgcaaag aaggtccgtt attcagattg tcataatcaa aataattttc ggcgtcatag
     9601 ggagcgatcc agcgtgcaaa aatggccacc acaatcaata aaataacgaa taatgcggcg
     9661 gtcatcgcca tatgctgacg gcgaaatcgt cgccagaatt catgccacgg tgtacgtacc
     9721 tggtcaggtt tgaccagtgg catggcgttt aacaccgcct gacgtcgcca gttaaatagt
     9781 cgcatcctta cttgtacctg atagccgggt taatggcggc gtaaagcaca tccaccacta
     9841 agttgataag aataaattcc agcgagaaaa gcagaatttc cgcctgaatc accgggtaat
     9901 cgcgcatttc tacagagtca acgagtaagc gtccaagtcc gggccagttg aaaactttct
     9961 ccaccacgat agaaccaccg agcaaaaagc caaactgtaa gcccatcatg gtcactaccg
    10021 ggatcatcgc gttgcgtagc ccgtgtttga ggacaaccca ggtttcgctc acccctttcg
    10081 ccctcgcggt acgcatataa tcttcgctta aaacgtcgac aaatgacgca cgggtaaagc
    10141 gcgccatcac ggcggccact gccgcgccga gcgtcaggga gggtaaaatg tagtgctgcc
    10201 aactgtctgc tcccacagta ggcagccagc ccagttcaac ggagaatacc tgaattaaaa
    10261 acatccccag cgcaaatgcc ggaaacgaga tccccgacac cgcaatggtc atactcaatc
    10321 gatccggcca acgattacgc cagacggcgg cgataattcc cgccgccata ccaaatatca
    10381 ccgcccagac catactggtt atggtcagcc atagcgttgg cataaagcgg ctggcaatct
    10441 catcggcaac cggacgacgc gacaccatcg acaggccaaa atccccctgc acagcattgc
    10501 tgatatagtg ccagaactgg tgatacagcg gctgatccaa ccccagctgc tgacgcacca
    10561 gttctataac ctgcgcatca gcttcgggcc cggcaatcaa tcgcgccgga tcgccgggca
    10621 gcatgtggac aaataaaaac accagcaccg agacgataaa cagcgtcgga atcaacccca
    10681 gtaatcgttt gataacgtaa taaagcattc cactccctgc gttgcttatt gcaaatccgc
    10741 gtcttcaaag ctgaagccgg tatctggcat gatccaaaaa ccggtcaggt ttttactgtg
    10801 tgccgacacc agtttttcta ccaccagcgg gatccacggc gattcttgcc agatgatatc
    10861 ctgcgccgcc ttatataagc gggtcttttc cgccggatca ttcgttttca gtgcctgagc
    10921 caggaagtca tccacctgtt tattgctgta aaacgcggta ttaaacagcg tcggtggcca
    10981 gttttgcgag gcaaacagcg gcgatagtgc ccagtcggct tcgccggttg aagccgacca
    11041 gccagtgtag aacatccgca cgccactctc tttttgccct ttaccttcaa cttctgccgc
    11101 ccgctgtccg gcatccatcg cggtcacctg ggctttaatc ccgacctgcg ctaactgctg
    11161 ctgggtaaat tgcagcactt tctgcgcggt gctgtggtta tgtgacgacc acagcgtggt
    11221 actgaaaccg ttgggatatc ccgcctcttt cagtaattcg cgcgctttca ctggatcgta
    11281 aggccacggt ttataacttt gcgcgtaggc gatacttggc ggtaccacac cagtagctgg
    11341 agttgcatag cccgcaaagg caactttcac cagcgccgga cggttaatgg cgtaattcag
    11401 cgcctcacgg accttcgggt tatcgaacgg cttttgcgtc acgttcatac tgatataacg
    11461 ctgcataatt gacggactgg ccatcaactc gatatttttg tttttctcca gcagtgtggc
    11521 ctgctcgtag ggaatgggga aagcaaactg cgcttcaccg gtttgcagca ttgccgcgcg
    11581 ggtgttgtta tccgccaccg gacgccaggt tatgctgtcc agtttgggca atcctggctg
    11641 ccagtaaccc gcgaattttt tcaccttcac aaaatcggtc tgattccagg tatccagttc
    11701 atacggtccg gttcccaccg gatgaaaacc aatctccttg ccatattttt ccagcgctgt
    11761 cggtgaaatc atcgcggtcg ccggatgggc aagaatatta ataaacgctg agaacggctg
    11821 tttgagggta atctttaccg ttgtcggatc gatcgcctcc gttttggcaa tattcttata
    11881 caggttatag cgtttaagat gattcgccgg atcgctggcc cggtccagat tcgcttttac
    11941 cgccactgcg ttgaaatcgg tgccatcctg gaatttaatt ccttcccgca atttcacggt
    12001 gtaagtaatg ccgtcatcgg aaacggtata actctccgcc agcacgtttt tcagtttcat
    12061 ctctttatcc agaccgaaca gcccctggta aaacgatttc gctacggcct gagataacgt
    12121 gtcattcgcg tcatacggat cgagcgtggt gaaattcgat cctaccgcca ccaccacatc
    12181 tttggcagcg aatgcacaag atgccatcaa cgctgtcgca atgcccagcg ccactaaccc
    12241 actacggtgt acagctcttg ccattttatt ctccgcctga atgttatcta cgcatgaatg
    12301 cgtattctga ttgtggttgt gcgacgtaat gccccggccc gacgcattgc aacgagacgg
    12361 ctgccacctc ttcgccacgc agatgaatat tgctgggaag atcgtccgac agcagtacac
    12421 gctgcggtcg ttgtcgggac ggttcagcga ccggaactgc cgccagtaat ttacgcgtat
    12481 aaggatgctg cgggttttcg aagaccgcgc accgtgaacc aatttcaaca atttgcccga
    12541 gatacatcac cgccacacga tgactaatcc gctctaccac agccatatcg tgggagataa
    12601 acagatacgc aatgccgaaa tcacgctgga gatcgagcaa caagttgata atctgcccgc
    12661 gaatagaaac atccagcgcc gaaacggctt cgtcggcaat gatcactttt ggattcaatg
    12721 ccaacgcgcg agcaatgcag atgcgctggc gctgaccgcc ggaaaactca tgcgggtaac
    12781 gccaggcatg ttcaggtaac aggcccacgc gctccagcaa ccacgcaacg cgtgcagccg
    12841 cgtctttacc tggcaataaa ccgtgtacac gcagcggttc gataatcgaa tcaccgatgg
    12901 tctgacgtgg gtccagcgaa gcgtaagggt cctgaaaaat aaactgaata tcccggcgca
    12961 atgcctgaag tttgccaggt gacaaggtat cgattcgctg accgttaaag ataatttcgc
    13021 cgccctgcga ttcgaccagg cgcagcaacg cccgcccggt agtggattta ccgctgccag
    13081 actcgcccac cagcgatagc gtttcgccag gccagagatc aaaactgact ttctcaacgg
    13141 catgcacttc ccgcgttacg cgattcaaca aaccgctgcg caaagggaaa cgggtgacaa
    13201 gattacgcac ccgtaaaaca ggttcgccat ccaccaccgt tttctgctcg atggggggtt
    13261 cctgtttcgc tggatgttca agcgatatca atgggaaacg tcggggataa tctaaccctt
    13321 tcatcgcacc aagttgcgga acagcagcta acagcgcacg ggtgtaagga tgttgcggtg
    13381 catgaaaaat ctgttcgacg gtacccgttt ccaccgcctc gccctgatac atcaccagta
    13441 cccgatcggc aatctctgcc accacgccca tatcgtgagt gataaagata acgcccatcg
    13501 acatctcttt ttgcaatact ttgattaatt gcaggatctg cgcctgaata gtgacatcca
    13561 gcgcggtggt tggctcatcg gcaatcagca ccgccgggcg gcatgacagc gccatcgcaa
    13621 tcatcactcg ctggcgcatc ccgccagaga gttgatgcga ataacgtgaa agaatggttt
    13681 gtgcctccgg aatgcgtacc tgatccagca tccgcttcgc ctcgaccatc gcttcttcac
    13741 gactggcgtt ctgatgcaga cgaattgatt cggcaatctg ttcacccaca gtaaatactg
    13801 ggttcagcga tgtcatcggc tcctgaaata tcatcgccat atccgcaccg cgcacatggc
    13861 gcatttgtgc agcgctctgc tcgctaagtt caatcacatc acgactgcgc cgccgcaaca
    13921 gcattttatc gcactgtact aaaccgcccg cctgttccaa cagacgcatc aatgccagcg
    13981 cagtcactga cttaccggag ccggattcgc ctacaattgc cagcgtctca ccgcgttgca
    14041 gactaaaaga gagattgcgg accgcagcta ttttctgctg gtcctgcata aaggcaatat
    14101 taagattttc aaccgccagc acattaccgg catcaagttc atcactgtgt ggcaacggtg
    14161 tccccttttt cacggtagat accggtggtt ggcgtatcgc ctgcgtagcc ccaggcgcga
    14221 tacattcctt cggtgttaaa cggtagcgcg acattccctt catggtcgat agcgattaag
    14281 ccaccgctac cgccaagcgc agggagtttt tccattacta cccgctcgca ggcttccgcg
    14341 agacttaatc cgccgtaatc cattaacgcg gcgatgtcat atgccgccag cgcgcggatg
    14401 aagacttcgc ctgtgccggt acaagaaacc gccacactgg cgttattggc gtagcatccg
    14461 gcacccacta agggactatc gccaactcgt ccgggtaatt tattggtcat tccgcctgtg
    14521 gacgtggctg ccgccagatt gccgtctaaa tccaacgcca cggcccccac ggtgcccatt
    14581 ttctgttttt catccagtgg cgcaccgcta tggtcgagga ctgttgcccc ttcctcgcgc
    14641 gctgccagta gttgttcata acgcaaaggc gtggagaaaa tctccggtga gacgcgctcc
    14701 atgccatgag caaacgcaaa attttctgcc ccttcgccaa tcatcatcac atgcgggctt
    14761 tgctccatca ccagccgggc ggcaagaacc ggattacgca gatgactaac gcccgccacc
    14821 gcaccggctt tcagggtgtt accatccatc acacaggcgt ccagttcatg ggtttcatca
    14881 cgcgtaaaga cagcgccaat tccggcgtta aacagtggac actcttccag cagacgcacc
    14941 gcttccgtca ccacatccag cgcactttcg ccggcttcca gcattttctg cccggtttca
    15001 acaatggcag acaacgcctc gatgtagcgt aattcctgtt gcagactcat ctgcgcgcgg
    15061 ctaattgcac ctgcgccacc atgaattgca atgactgctt tgcccatagt attcgtccat
    15121 taagggtggc tcgccacttt tgctataaat atcagaattt ttatgaatta ctcattcaat
    15181 ttttgaatat agaaagatgg atatggcatg taaaggcagg agccaacgct acgcccgagg
    15241 tggcatacag tctatggcgt ttttctgaca taataggcaa attcgatttt gcctccgcag
    15301 gagtgttttc atggaattta ccaccggatt gatgtcgctc gacaccgcgc ttaatgagat
    15361 gctttctcgc gttaccccac tgaccaccca ggaaacgctg ccactggttc agtgttttgg
    15421 tcgtattctg gcgagcgatg tcgtttcgcc actggatgtc ccggggtttg ataactccgc
    15481 tatggacggc tacgcggtgc gtttagccga tattgcctcc gggcaaccgc tgcccgttgc
    15541 cggtaaatcc tttgccggtc agccatacca tggtgaatgg cctgcgggta cctgcattcg
    15601 tattatgacc ggtgcgccgg tgccggaagg ctgcgaagcg gtggtgatgc aggagcagac
    15661 tgaacaaacg gacaatggcg tgcgttttac tgctgaagtg cgtagcgggc aaaatattcg
    15721 ccgtcgcggt gaagatatct ctgcaggtgc ggttgttttc ccggcgggaa ctcgcctgac
    15781 taccgcagag ctgccagtaa ttgcttcact ggggattgcc gaagttccgg tgattcgtaa
    15841 agtgcgtgta gcgctttttt ctaccggtga tgaactccag ttgcccggtc agccgctggg
    15901 cgacggccaa atctacgaca ccaaccgtct cgccgtacac ctgatgttag aacagctggg
    15961 ttgcgaggta atcaacttag ggattatccg cgacgatcca catgccctgc gcgccgcatt
    16021 tattgaagcc gacagccagg cggatgtagt gatcagttcc ggcggtgttt cagtgggtga
    16081 agcggattac accaaaatga ttcttgaaga gctgggggag atcgccttct ggaagctggc
    16141 gattaaacca ggtaaaccgt tcgcgttcgg taaactcagc aatagctggt tctgcggcct
    16201 gccgggcaac ccggtttcag cgacgctgac cttctatcaa ctggtacagc ctttgctggc
    16261 aaaactaagc ggcaacaccg ccagcggcct gcccgcgcgc cagcgcgtac gcacagcgtc
    16321 ccgcctgaaa aaaacgccag gacgtcttga tttccagcgc ggcgtgctgc aacgcaacgc
    16381 cgacggtgaa cttgaagtga cgaccaccgg acatcagggt tcacatatat ttagctcctt
    16441 tagtctcggc aactgcttta tcgtgctgga acgcgatcgc ggcaatgtgg acgtgcgcga
    16501 atgggtggaa gtagaaccgt ttaacgcgtt gttcggaggc ctgtaatggc ggaactcagc
    16561 gatcaggaga tgctgcgcta caaccggcaa atcattctgc gcggttttga ttttgacggt
    16621 caggaggcgc taaaagattc tcgtgtgctg gtagtcggcc tcggtggact cggctgcgca
    16681 gcttcgcagt atctggcaag cgccggtgtc ggcaacctga cgctgctcga cttcgacacg
    16741 gtttcgctct cgaatctgca acgccagaca ctgcacagtg atgccacggt cgggcaaccg
    16801 aaggtggaat ccgcccgtga cgccctgacg cggatcaacc cacatatcgc gattacgcca
    16861 gtcaatgcac tgctagatga cgcagaactt gcagcattga ttgctgaaca cgatctggtg
    16921 ctcgactgta cggataacgt tgcggtacgt aatcaactga acgcaggctg ttttgccgcg
    16981 aaggtgccgc tggtttccgg cgcggcaatt cgtatggaag ggcaaatcac ggtcttcact
    17041 tatcaggacg gcgaaccgtg ctatcgctgc cttagccgtt tgtttggcga gaacgcgtta
    17101 acctgcgtag aagcaggcgt gatggcaccg ttgataggcg taattggttc gttgcaggca
    17161 atggaagcga tcaaactgct ggcaggttat ggcaaaccgg ccagcgggaa aatcgtcatg
    17221 tacgatgcga tgacctgtca gtttcgcgaa atgaaactga tgcgcaatcc ggggtgtgag
    17281 gtgtgcgggc agtaattgcc acctccaagc ctgataagac gcgacaagcg tcgcatcagg
    17341 catttgctcc agacgccaga tgcggcgtga acgccttatc tggcctacgg ttcgggcaca
    17401 gatttccaag cgcggcaggc atcggtgcac tccgccacgt catcagcaat ttatgaggat
    17461 gcagaacgtc cggttaaatc gacgttctgc caaacgctcc ctgccagtcc tgctcaaact
    17521 tcgtcacagc ggcttccacc gccggatagc taatcatctg ttgtgccaca tccagtggca
    17581 gagtaattga ttcacatccg gccagtaagc agtccagcgc ctgacgcggg gttttgaaac
    17641 tcgctgccag cactttcgcc tgcggcgcat gcattttcaa taactggtgt aagtcggtca
    17701 cagtctgaat gccgctaccg ccctgagcat caatacgatt aacgtaaggc gcaacatatt
    17761 ccgcacctgc cagcgccgac agcagccctt gtgccgcgcc atataccgcg gttcccagcg
    17821 tcggaatccc ttccgctttt aacatcttaa tcgctgccag cccctcggct gtcaccggaa
    17881 ctttcaccac gatatccgca ataatagaac gcagcttaag cgcgtcattc accatccctt
    17941 cagcagtggt agccattacc tgggcaaaca gacgcccctg accgcccatc gcttcatgaa
    18001 gttgcggaag cacaacatcc agcggttttt tacccgcggc gataatgctt gggttagtgg
    18061 tcacacccgc cagcggaaaa atacgtgaca gcgccttcac cgcaacaacg tctgaagtat
    18121 ccagatacag ttccataacc atcctcaaaa tgtacttttt gtctgaatgt agactatcac
    18181 gggaaaactc tctgaacagt tgacgcacat caatctaact ttcattcgaa agtattttaa
    18241 tgtttgaacg aaacagagag gcaactatga ttttcaatat tcagcgctac tcgacccatg
    18301 atggccccgg tatccgcacg gtcgtatttc ttaaaggctg ttcgctgggc tgccgctggt
    18361 gtcagaaccc ggaaagccgc gcccgtacgc aggatctgct gtatgacgca cgactgtgtc
    18421 tggaaggctg cgagctgtgc gctaaggccg cgccggaagt gattgagcgc gcgctgaatg
    18481 gtttgcttat tcatcgggaa aagttaaccc cggagcatct gacggcgtta accgactgct
    18541 gtccgacaca ggcgttaacc gtgtgtggtg aagtgaaaag cgttgaggag atcatggcga
    18601 ccgttctgcg cgataaaccg ttttacgatc gcagcggcgg cggcttaacg ctttcgggtg
    18661 gtgaaccctt catgcagccg gaaatggcga cggcgctgct gcaagcctgc cacgaggcag
    18721 gcattcatac tgcggtagaa acctgtctgc atgtgccgtg gaaatatatc gccccttctc
    18781 tgccctatat cgatctgttt cttgccgatt taaaacacgt tgccgacgcg ccgtttaaac
    18841 agtggaccga cggtaacgcc gccagggtgc tggataacct gaaaaaactc gccgcagcgg
    18901 gcaaaaaaat cattatccgc gtgccgctga ttcagggctt taatgccgac gaaacctctg
    18961 taaaagccat taccgatttt gccgccgacg agctgcacgt tggcgaaatt cattttctgc
    19021 cctaccacac gctgggcatc aacaaatatc acttacttaa tctgccctat gacgccccgg
    19081 aaaaaccgct tgatgcgcca gagctgctcg attttgccca gcagtatgcc tgccagaaag
    19141 ggttaaccgc gaccttacga ggataacaac catgaccaca ctgaaactgg acacgctcag
    19201 cgaccgcatt aaagcgcaca aaaatgcgct ggtgcatatt gtgaagccgc cagtctgtac
    19261 cgagcgcgcg cagcactata ccgagatgta tcaacaacat ctcgataagc cgatcccggt
    19321 acgtcgcgcg ctggcactgg cgcatcacct ggcgaatcgc accatctgga tcaaacacga
    19381 tgagttgatc attggcaacc aggcaagcga agttcgcgcc gcgccgatct tcccggaata
    19441 tactgtctcg tggatcgaaa aagagattga tgatctggca gatcgtcccg gtgctggctt
    19501 tgcggtgagc gaagagaaca aacgtgttct gcatgaagtg tgcccgtggt ggcgcggtca
    19561 gacggtacag gatcgctgct atggcatgtt taccgatgag caaaaaggtc tgctggcgac
    19621 cggcatcatt aaagcggaag gcaatatgac ctccggcgat gcgcacctgg cggtcaattt
    19681 cccgctgctg ctggaaaaag ggcttgatgg tctgcgtgag aaagtagcgg aacgtcgctc
    19741 gcgcatcaat ctgacggtgc tggaagattt gcacggcgaa cagttcctga aagcgattga
    19801 tatcatgctg gtggcagtca gtgaacacat tgaacgtttc gctgccctgg cgcgtgcaat
    19861 ggccgcgacc gaaacccgcg aaagccgtcg cgatgaactg ctggcgatgg cagaaaactg
    19921 cgatcttatc gcccaccagc cgccgcagac tttctggcag gcgctgcaac tgtgttactt
    19981 catccagttg attttgcaga ttgaatctaa cggtcactcg gtgtcgtttg gtcgtatgga
    20041 tcagtatctc tacccgtact atcgccgcga cgttgaactg aaccagacgc tggatcgcga
    20101 acacgccatc gaattgctgc atagctgctg gttgaaattg ctggaagtga acaaaatccg
    20161 ctccggctcc cactcaaaag cctctgcggg aagtccgctg tatcagaacg tcaccattgg
    20221 cggacaaaat ctggttgatg gtcaaccaat ggacgcggtg aatccactct cttacgcgat
    20281 cctcgaatcc tgcggtcgcc tgcgttccac tcagcctaac ctcagcgtgc gttaccatgc
    20341 aggaatgagt aacgatttcc tcgacgcctg cgtacatgtg atccgctgcg gcttcgggat
    20401 gccggcattc aacaacgacg aaatcgtgat cccggaattt attaaactcg gtattgaacc
    20461 gcaggacgct tatgactacg cagcgattgg ttgtatagaa accgccgtcg gtggcaaatg
    20521 gggctatcgc tgtaccggca tgagctttat caacttcgcc cgcgtgatgc tggcggcgct
    20581 ggaaggcggg cgtgatgcca ccagcggcaa agtgttcctg ccacaagaaa aagcgttgtc
    20641 ggcaggtaac ttcaacaact tcgatgaagt gatggacgcg tgggatacgc aaatccgtta
    20701 ctacacgcgt aaatcaatcg aaatcgaata tgtcgtcgac accatgctgg aagagaacgt
    20761 gcacgatatt ctctgctcgg cgctggtgga tgactgtatt gagcgagcga aaagtatcaa
    20821 gcaaggcggc gcgaaatatg actgggtttc tggcctgcag gtcggtattg ccaacctcgg
    20881 caacagcctg gcggcagtga agaaactggt gtttgaacag ggcgcgattg gtcagcaaca
    20941 gctggctgcc gcactggcgg atgacttcga tggcctgact catgagcaac tgcgtcagcg
    21001 gctgattaac ggtgcgccga agtacggcaa cgacgatgat accgtcgata cgctgctggc
    21061 tcgcgcttat cagacctata tcgacgaact gaaacagtac cataacccac gctacggtcg
    21121 tggtccggtt ggcggcaact attacgcggg tacgtcgtct atctccgcta acgtaccgtt
    21181 tggcgcgcaa actatggcaa cgccggacgg gcgtaaagcc cacaccccgc tggcagaagg
    21241 cgctagtccg gcctctggta ctgaccatct cggccctact gcggtcattg gctcagtggg
    21301 taaactgcct acggcagcga ttctcggcgg cgtgttgctc aaccagaaac tgaatccggc
    21361 aacgctggag aacgaatctg acaagcagaa actgatgatc ctgctgcgta ccttctttga
    21421 agtgcataaa ggctggcata ttcagtacaa catcgtttcc cgcgaaacgc tgctggaggc
    21481 gaaaaaacat ccggatcagt accgcgatct ggtagtgcgt gtcgcgggtt attccgcctt
    21541 cttcaccgcg ctctctccag acgctcagga cgatatcatc gcccgtactg aacatatgct
    21601 gtaatctctt cggcccggct gcggcgtcgc cgggctaaat cattcacatc atcaatttca
    21661 tccttacttt cattcgaaat ataatttgtg ctctgcgtca cattgttatt acactgttac
    21721 ggtttcattt tcgagccagg agcacaccta tgagcgtaaa agttatcgtc acagatatgg
    21781 acggtacttt tcttaacgac gccaaaacgt acaaccaacc acgttttatg gcgcaatatc
    21841 aggaactgaa aaagcgcggc attaagttcg ttgtcgccag cggtaatcag tattaccagc
    21901 ttatttcgtt ctttcctgag ctaaaggatg agatctcttt tgtcgcggaa aacggcgcac
    21961 tggtttacga acatggcaag caactgttcc acggcgaact gacccgacat gaatcgcgga
    22021 ttgttattgg cgagttgcta aaagataagc aactcaattt tgtcgcctgc ggtctgcaaa
    22081 gtgcatatgt cagcgaaaat gcccccgaag catttgtcgc actgatggca aaacactacc
    22141 atcgcctgaa acctgtaaaa gattatcagg agattgacga cgtactgttc aagttttcgc
    22201 tcaacctgcc ggatgaacaa atcccgttag tgatcgacaa actacacata gcgctggatg
    22261 gcattatgaa acccgtcacc agtggttttg gctttatcga cctgattatt cccggtctac
    22321 ataaagcaaa cggtatttcg cggttactga aacgctggga tctgtcaccg caaaatgtgg
    22381 tagcgattgg cgacagcggt aacgatgcgg agatgctgaa aatggcgcgt tattcctttg
    22441 cgatgggcaa tgctgcggaa aacattaaac aaatcgcccg ttacgctacc gatgataata
    22501 atcatgaagg cgcgctgaat gtgattcagg ctgtgctgga taacacatcc ccttttaaca
    22561 gctgacctct ctaccggagc catgcgctcc ggttttccct tctttttaca tcctgtacat
    22621 cacatcaaat cattaaactt gcattaactt atttataact tgtattgtca catgcaaatt
    22681 agcatagttt cgaatgaaag ttaatgcaag gtggttatgg cttctacttt taccagcgac
    22741 acattgcctg ccgatcacaa agcagctatc cgtcagatga agcacgcgct gcgggcgcag
    22801 cttggcgatg tccagcagat ctttaatcag ctaagcgatg acattgccac gcgagtggct
    22861 gaaatcaacg cactcaaagc acagggcgat gccgtctggc cggtgctgtc ttatgccgat
    22921 atcaaagcag gtcatgttac tgcagagcag cgcgaacaga ttaaacgtcg cggttgtgcg
    22981 gtgataaaag gccatttccc ccgcgaacaa gcgctaggct gggatcagtc gatgctggac
    23041 tatctgaacc gcaaccgctt tgacgaggtc tacaaaggcc ccggcgataa tttcttcggg
    23101 acgctcagcg cttcacgtcc cgagatttac cccatctact ggtcgcaggc gcaaatgcag
    23161 gcccgccaga gtgaagaaat ggcgaatgcg cagtcgtttc tcaatcgtct gtggacattt
    23221 gaaagtgatg gaaagcaatg gtttaacccg gatgtgagcg tcatctaccc tgaccgtatc
    23281 cgcctccttc cgcccggaac gacctccaaa ggtcttggag cgcataccga ctccggggcg
    23341 ctggaacgct ggctgcttcc agcgtatcag cacgttttcg ccaacgtctt taatggcaat
    23401 ctggcgaaat acgatccctg gcatgcggca catcgtacgg aagttgaaga gtacacggtg
    23461 gacaacacca ccaaatgttc cgtgtttcgg acattccagg gctggactgc gctctcggat
    23521 atgctgcctg gtcaggggtt gctgcacgtc gtgcccattc ctgaagccat ggcgtacgta
    23581 ctgttacgtc cgctgcttga tgatgtgccg gaggatgaac tgtgcggcgt agcgcccgga
    23641 agagtgttgc cggtatcaga gcaatggcat ccactgttaa ttgaggcgtt aaccagcatt
    23701 ccaaaactcg aggccggaga ctccgtctgg tggcactgcg acgtcatcca ttccgttgcc
    23761 cccgttgaaa atcaacaggg ttggggcaac gtgatgtaca ttcctgcggc accgatgtgc
    23821 gagaaaaatc ttgcctacgc gcacaaggtg aaggccgcac tggaaaaagg cgcatcgccg
    23881 ggcgacttcc cgcgcgagga ctatgaaaca aactgggaag gacgctttac gcttgccgac
    23941 ctcaacattc acggtaagcg agcgttgggc atggatgttt gagagttaat ggaagtgaat
    24001 tgatgtggga ttaagaaaat cgattcgacg ttactcagat tgctgacaag tgcgctttct
    24061 taatgcctga tgcggcgtta acgccttatc cggcctacaa agtcctgcag attcaatata
    24121 ttacaggtac tgcgtaggcc tgataagcgt agcgcatcag gctattttgc atttgtcatc
    24181 agccgtcgtc accccgacag tatcggggtg acggataatc acttactcga tccctttact
    24241 acgcaggtaa tcttcgtaat taccgctaaa gtcgatcacg cgttccgggg tgatttccag
    24301 aatgcgggtc gccagggagc ttacgaactc acggtcgtgt gaaacaaaga tcagcgtgcc
    24361 ctgatacagt tccagcgcca tgttcagtga ctcgatggat tccatatcca ggtggttggt
    24421 cggttcgtcc atgatcagaa tgttcggctt ctgcatcatt aacttaccaa acagcatccg
    24481 ccctttttcc ccaccggaaa gcactttagc tggctttttg atgtcgtcct ggctgaacag
    24541 caaacgaccg agaatgctgc gtaccgcctg ctcgtcatcg ccttcctgct tccactgact
    24601 catccattcg aacacggtca gatcattttc aaactcatat tcgtggtcct gggcgtagta
    24661 accaatgcgc gcgttttcag accattttac ggtgccgctg tccggttgca gatcgcccac
    24721 cagcgttttc agcagcgttg atttaccgac gccgttggta cccagtaccg caagtttttc
    24781 accgacctcc agcagcaggt tgagattttt aaacagtgga ccgttatcaa accctttggt
    24841 cagaccttcc acttccagcg cgttacggaa cagtttctta tcctgttcaa aacggataaa
    24901 cgggttctga cggctggagg ctttcacctc ttccagtttg attttatcaa tctggcgcgc
    24961 gcgcgaagtt gcctggcgag atttcgaggc gttggcgcta aagcggctaa cgaaagattg
    25021 caactcagca atctgcgctt tcttcttggc gttatcggcc agcagacgtt cacgcgcctg
    25081 ggtcgccgcc gtcatgtact catcgtagtt acccggataa acgcgcagct cgccgtaatc
    25141 cagatccgcc atgtgggtac agaccatgtt aaggaagtga cggtcgtgcg agatgatgat
    25201 catggtgctg tcacgctcgt tcagcacctg ttccagccag cgaatggtgt cgatgtcgag
    25261 gttgttggtc ggttcgtcga gcaggagaat atccggctca gcaaacagcg cctgcgccag
    25321 aagcacacgc agcttccagc caggagcaac ttcactcatc gggccgtagt gctgttccac
    25381 tggaattccc acgccaagca acagttcacc ggcgcgagct tccgcagagt aaccgtccat
    25441 ttcgccgtat ttcacttcca gatcggccac tttatagccg tcttcttcac tcatttccgg
    25501 caaagcatag atgcggtcgc gctcctgctt cacttcccac aactctttat gccccatgat
    25561 caccgtatcc agcacagtga actcttcaaa ggcaaactga tcctgacgca gcttaccaat
    25621 gcgctcgttg ggatcgaggg aaacgttacc cagcgtcggc tcaaggtcgc cgccgagaat
    25681 cttcataaag gtggatttac cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc
    25741 gccgaatttg acggaaatgt tttcaaacaa cggcttactg ccgaactgca tggtgacgtt
    25801 actggaaact aacacaggcg tatcctgaaa agagatatga caaaccgcgc attatgccat
    25861 aagtctgaaa gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa
    25921 tgtgatttcg tacacatctg atttcactgt gagctggaat gaacttataa tgcgcttcca
    25981 atactctaat attctcaacc caatggcctg ccaggcacaa aatctcgctt aacatgaata
    26041 tgaaattgaa aacattattc gcagcggcct tcgctgttgt cggcttttgc agtaccgcct
    26101 ctgcggtaac ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga
    26161 tcaccattcc tgaaggtaac actcagccgc tggagtattt tgccgcggag taccagatgg
    26221 ggctttccaa tatgatggaa gcgaacccgg gtgtggatac cttcctgccg aaaggcggta
    26281 ctgtactgaa cattccgcag cagctgatcc tgccggatac cgttcatgaa ggcatcgtca
    26341 ttaacagtgc agagatgcgt ctgtattact atccgaaagg gaccaacacc gttatcgtgc
    26401 tgccgatcgg cattggtcag ttaggcaaag atacgcctat caactggacc accaaagttg
    26461 agcataagaa agcaggcccg acctggacgc cgaccgccaa aatgcacgca gagtaccgcg
    26521 ctgcgggcga accgctaccg gctgtcgttc cggcaggtcc ggataacccg atgggtctgt
    26581 atgcactcta catcggtcgc ctgtatgcta tccatggcac caacgccaac ttcggtatcg
    26641 gcctgcgtgt aagtcatggt tgtgtgcgtc tgcgtaacga agacatcaaa ttcctgttcg
    26701 agaaagtacc ggtcggtacc cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg
    26761 agccagacgg cagccgttat attgaagtcc ataacccgct gtctaccacc gaagcccagt
    26821 ttgaaggtca ggaaatcgtg ccaattaccc tgacgaagag cgtgcagaca gtgaccggtc
    26881 agccagatgt tgaccaggtt gttcttgatg aagcgatcaa aaatcgttcc gggatgccgg
    26941 ttcgcctgaa ttaatcgtca aaacgtaaaa caaaggcgga ctcataatcc gcctttttta
    27001 tttgccagac cttaccaggc cgggatcata actaacaaaa cgtatcccac cagcgccgcc
    27061 cacaacagca tcggtattga atagagatgg aaacgccacc agatgcggcg atcgttcgcc
    27121 atacgtagcg caatcaaatt tgccagcgat cctggtaata aaccaaagcc ccccacgttt
    27181 accgcccatg ccagtaataa agacggaggt acataattga gcagcaaaat ggtactcggc
    27241 acgttactga tcacctgcga taaaccgact gccgttaacc ataacccggg ttcagataaa
    27301 tgactcacgt tacccaacac gccttgcaac gctggaagct gggtcagtaa atggacgtcg
    27361 ataaacatcg ccataaacac cagcagcaac gtccagtcca cactcaacac cacgcgacgc
    27421 gccagcagca caaagcccgc cgccacaatc accagtcccc acagctcttg tttgaactcc
    27481 agcgccgtca gaaagacgat atacagcccc agacaattcc acaccagccg cggtttccac
    27541 tccggtgttt gcacccccgt atggtattgc agcgcctttc cagggaaaca acaccagcac
    27601 aacagcagga gtgtcagcat cattgcgcca gccatcggtg ccatttgggc aataaatccg
    27661 gcaaacgaaa gaccagaacg tccccagata agaatatttt gcgggttgcc aattggcgtc
    27721 agtagcgaac cagcgttgac tgccagcgcc tcaaaaataa tcagccgatt aaccgggatc
    27781 tcacacagtc tttttagcgt gatagtcagc ggaacaacaa taaacagcgc gacatcgttg
    27841 gtcagaaagg tagaaagcag cgccgccgcc agcaccataa acatcgccag ccgacgctcc
    27901 gtagcaaagc ggcgcaccat tttgcgcccc agcacatcaa aataaccgct taactccaca
    27961 cctttggtca gcagcatcag gccgcttaag gtgatgatgg tgtgccagtc gatagcagca
    28021 ggccaggatt tcggtgcaaa gggcacgaaa aagcttaatc cgataccaac aagaattaat
    28081 aactgaaaaa acgatcgcct tgcagcgtgc gtaaaaaagg caggctcatt tggcaccgtg
    28141 tttctgggta aacaaacgaa aagcgtcgag cgtctcttca ctaacatggt gctccatgcc
    28201 ttccgcgtcg cgacgggcga tttccggact gacgcccaac accaacaaga aattttcgac
    28261 tatctgatgt cgctcgcggc tttcctgcgc cagcttctct ccttctgccg ttaaaaacac
    28321 gccacgccag gggatcattt caatcagccc catggttgcc agccgcttaa gcattttagc
    28381 caccgtcggt tgcgaaactc ccagacgagc agccatgtcc acctgacgag cttcccccac
    28441 ttccctgatc aagtcagaaa tcagctcaac gtaatcatca ataagctcgc gccgatgcgc
    28501 ctctctgacc tggcggaacc cttcaacgtg ctcttccacg ttcactaact gcgtcacttt
    28561 ttttgctatt ggcgtacctg cgcgacgact cattgtgctt cctcattatg gtgacgcata
    28621 catagcgttc tcatttcgtg cgcacattgt aaaccagagt tgcgaaggta caaaaaatta
    28681 acgttttagc aatagctata taatatagcc tgtgctatat ctgtatgtaa tgcaatcatc
    28741 cctcaaggat cgacgggatt agcaagtcag gaggtcttat gaatgagttc aagaggtgta
    28801 tgcgcgtgtt tagtcattct ccctttaaag tacggttaat gctgctctct atgttgtgcg
    28861 atatggtcaa caacaaaccg cagcaagata aaccttcaga taaatagcgg cgtcgcggta
    28921 cgccgcttca ctcctgcttt tatgcaggca taacgcgttt tggtctgaaa aaaaccactt
    28981 tttgtcggat ttgcaatccc cttcgcaaaa gatttgttcg tcagtagttg acctgaacgg
    29041 cggctcgctc tatcttcttg cagccctgcg tatattgcgg ctcgcggatg cggacccctt
    29101 tcccctcttc acgcactctt gcaggtattg acccttgacg ccagggtaag cacatggcgt
    29161 ttgttacgat agtggcatat gaatttaacc ctcaaagaat cgcttgttac ccgtagccgg
    29221 gtatttagcc cgtggactgc gttctacttt ttacagtcgc tattaattaa cctcggctta
    29281 ggttacccct tcagtttgct ctacaccgct gcgtttacgg ctattttgct tttgctatgg
    29341 cgaacattgc ctcgcgtaca aaaagttctg gtcggtgtca gttcgctggt ggcggcttgt
    29401 tatttccctt ttgctcaggc ctacggcgcg cctaatttca atacattgct ggcattgcac
    29461 tccaccaata tggaagagtc gaccgaaatc ctgacgattt ttccgtggta cagctacctg
    29521 gtcggcttat ttatttttgc gctcggcgta atagcaatca ggcgaaaaaa agagaatgaa
    29581 aaagcgcgct ggaatacctt cgacagcctg tgtctggtat tcagtgtggc gacatttttc
    29641 gttgctcccg tgcaaaacct ggcctggggt ggcgtattta aactgaaaga tactggctat
    29701 ccggtatttc gttttgctaa ggatgtcatc gtcaataata acgaggtgat tgaagagcaa
    29761 gaacggatgg caaaactttc cggaatgaaa gatacctgga cggtcactgc cgttaagccg
    29821 aagtatcaga cctatgtggt agtgatcggt gaaagcgcgc gtcgcgatgc cctcggtgcc
    29881 tttggcggtc actgggacaa taccccgttt gccagcagcg ttaacggttt gatatttgct
    29941 gactacattg ctgccagtgg ctccacgcag aaatcgcttg gcttaacgct caatcgcgta
    30001 gtcgatggca aaccacagtt tcaggataac tttgtcaccc tggcaaatcg cgcgggcttc
    30061 cagacctggt ggttttccaa ccagggccaa atcggcgaat acgatactgc tatcgccagc
    30121 atcgccaaac gtgcagatga agtgtacttc ctgaaagaag gtaattttga agcagataaa
    30181 aacacgaaag acgaagcgtt actggatatg accgctcaag tgctggcgca agagcactcg
    30241 caaccgcagc tgattgttct gcatctgatg ggctcgcatc cgcaggcctg cgacaggaca
    30301 caaggcaaat acgaaacctt tgtgcaatcg aaagaaacgt cgtgctatct ctataccatg
    30361 acgcaaacgg acgatttact gcgcaagctg tacgatcagt tacgcaacag cggcagcagc
    30421 ttctctctgg tttacttttc tgaccacggt ctggccttta aagagcgcgg caaagacgtg
    30481 caataccttg cccatgatga taaatatcag caaaatttcc aggtgccttt tatggtcatt
    30541 tccagcggcg ataaagcgca tcgggtgatt aaagcccgcc gctcagccaa tgacttctta
    30601 ggctttttct cgcagtggac gggaattaaa gcgaaggaaa taaatatcaa atacccgttt
    30661 atatctgaga agaaagccgg gccgatatac atcaccaact tccagttaca gaaggtggat
    30721 tacaaccatc tcggaaccga tattttcgac ccgaaacctt aaaaacaaaa atccgccccg
    30781 agaggcggat ttttatatca ccaaagtgat tagaagcggt aaccaacacc ggcaatccag
    30841 gtgcctacgt caacgctacg aatacggctc tgctcgtaag agaagtccag agcaacgttt
    30901 tccatcgggt tgaactgcag accagcaccg taggagaaac cgtagtcgct ggtgtcgtgt
    30961 ttgtaggtcg ggtattcagt ggtctggaat ttaccataac ccacacccac tacaccgtag
    31021 atgcttgccc agtcgttaat gcggtaagcc ggaccagcag tgatgccgta gtactggttt
    31081 ttgttgtagt caccagagct tgcagtacgg cttttctcgg tgtaagtgaa agaaccgatc
    31141 acacccagcg ggctgttgtc ttcttcatag cggtatttca ggttgaaacc gcccattttg
    31201 ttcatttggc cctgagcgtc gctctgtgcg taaccgccag ttacggtaga agtcgcagct
    31261 acggaagtac ctgcggtgaa agccagaact gcggccagtg ctgaaagaca tgcaattttt
    31321 ttcataacca cctcaaatgt gattcaaata agtcctaagt tttaaatata tcaaaaatta
    31381 atgggaaact cttcgcgatt tgtgatgtct aacgggccat ttcatgtaac agaacgtttc
    31441 catacaccgc tatccatcta aatttaaatc actttttcag agaactgcgt aagtattacg
    31501 catgttttcc ctgtcattca tccagattat tcctaatcac cagactaatg attccatcaa
    31561 tcctggcgca ttttagtcaa aacgggggaa aattttttca acaaatgctc aaccagcatt
    31621 gggtatatcc agtacactcc acgctttact taagtctaga tatttgtggg agaaaggatg
    31681 cctggttcat tacgtaaaat gccggtctgg ttaccaatag tcatattgct cgttgccatg
    31741 gcgtctattc agggtggagc ctcgttagct aagtcacttt ttcctctggt gagcgcaccg
    31801 ggtgtcactg cgctgcgtct ggcattagga acgctgatcc tcatcgcgtt ctttaagcca
    31861 tggcgactgc gctttgccaa agagcaacgg ttaccgctgt tgttttacgg cgtttcgctg
    31921 ggtgggatga attatctttt ttatctttct attcagacag taccgctggg tattgcggtg
    31981 gcgctggagt tcaccggacc actggcggtg gcgctgttct cttctcgtcg cccggtagat
    32041 ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc tgctaccgct ggggcaagac
    32101 gtttcccatg tcgatttaac cggctgtgcg ctggcactgg gggccggggc ttgttgggct
    32161 atttacattt taagtgggca acgcgcagga gcggaacatg gccctgcgac ggtagcaatt
    32221 ggttcgttga ttgcagcgtt aattttcgtg ccaattggag cgcttcaggc tggtgaagca
    32281 ctctggcact ggtcggttat tccattgggt ctggctgtcg ttattctctc gaccgctctg
    32341 ccttattcgc tggaaatgat tgccctcacc cgtttgccaa cacggacatt tggtacgctg
    32401 atgagcatgg aaccggcgct ggctgccgtt tccgggatga ttttcctcgg agaaacactg
    32461 acacccatac agctgctggc gctcggcgct atcatcgccg cttcaatggg gtctacgctg
    32521 acagtacgca aagagagcaa aataaaagaa ttagacatta attaaattta catttctgta
    32581 tggttatgca taaccatgca gaatttctcg ctacttttcc tctacaccgt atttatatat
    32641 cgaattatgc aaaagcatat ttattccgaa aattcctggc gagcagataa ataagaattg
    32701 ttcttatcaa tatatctaac tcattgaatc tttattagtt ttgtttttca tgcttgttac
    32761 cactattagt gtgataggaa cagccagaat agcggaacac atagccggtg ctatacttag
    32821 tctcgttaat tactgggaca taacatcaag aggatatgaa attatgagta ccgctaaatt
    32881 agttaaatca aaagcgacca atctgcttta tacccgcaac gatgtctccg acagcgagaa
    32941 aaaagcaaca gtagagttgc tgaatcgcca ggttatccag tttattgatc tttctttgat
    33001 taccaaacaa gcgcactgga acatgcgcgg cgctaacttc attgccgtac atgaaatgct
    33061 ggatggcttc cgcaccgcac tgatcgatca tctggatacc atggcagaac gtgccgtgca
    33121 gctgggcggt gtagctctgg ggaccactca agttatcaac agcaaaaccc cgctgaaaag
    33181 ttacccgctg gacatccaca acgttcagga tcacctgaaa gaactggctg accgttacgc
    33241 aatcgtcgct aatgacgtac gcaaagcgat tggcgaagcg aaagatgacg acaccgcaga
    33301 tatcctgacc gccgcgtctc gcgacctgga taaattcctg tggtttatcg agtctaacat
    33361 cgaataaatc catcgctgat ggtgcagaac tttagtaccc gataaaagcg gcttcctgac
    33421 aggaggccgt tttgttttgc agcccacctc aacgcactta tttagtgcat ccatctgcta
    33481 tccccagctg attaagtaaa ttttttgtat ccacatcatc acacaatcgt tacataaaga
    33541 ttgttttttc atcaggtttt acgctaaata atcactgtgt tgagtgcaca attttagcgc
    33601 accagattgg tgccccagaa tggtgcatct tcagggtatt gccctataaa tcgtgcatca
    33661 cgtttttgcc gcatctcgaa aaatcaatga gttgcaaaat tggcacgatt ttttcatata
    33721 tgtgaatgtc acgcagggga tcgtcccgtg gatagaaaaa aggaaatgct atgaagtctg
    33781 tattaaaagt ttcactggct gcactgaccc tggcttttgc ggtttcttct catgccgcgg
    33841 ataaaaaatt agttgtcgcg acggataccg ccttcgttcc gtttgaattt aaacagggcg
    33901 ataaatatgt gggctttgac gttgatctgt gggctgccat cgctaaagag ctgaagctgg
    33961 attacgaact gaagccgatg gatttcagtg ggatcattcc ggcactgcaa accaaaaacg
    34021 tcgatctggc gctggcgggc attaccatca ccgacgagcg taaaaaagcg atcgatttct
    34081 ctgacggcta ctacaaaagc ggcctgttag tgatggtgaa agctaacaat aacgatgtga
    34141 aaagcgtgaa agatctcgac gggaaagtgg ttgctgtgaa gagcggtact ggctccgttg
    34201 attacgcgaa agcaaacatc aaaactaaag atctgcgtca gttcccgaac atcgataacg
    34261 cctatatgga actgggcacc aaccgcgcag acgccgttct gcacgatacg ccaaacattc
    34321 tgtacttcat caaaaccgcc ggtaacggtc agttcaaagc ggtaggtgac tctctggaag
    34381 cgcagcaata cggtattgcg ttcccgaaag gtagcgacga gctgcgtgac aaagtcaacg
    34441 gcgcgttgaa aaccctgcgc gagaacggaa cttacaacga aatctacaaa aaatggttca
    34501 gtactgaacc gaaataataa cgctacacct gtaaagcgca ctggcagttc cctctccccc
    34561 ctggggagag gattagggtg aggggcgcaa acccgctccg gggccattaa ttaccctgaa
    34621 tttgattatt tacaccacgg taacaggaac aacatatgca gtttgactgg agtgccatct
    34681 ggcctgccat tccgcttctg attgaaggtg ccaaaatgac cctgtggatt tcggtcctcg
    34741 gtctggctgg cggtctggta atcggattgc tggcaggttt tgcacgcacc ttcggtggtt
    34801 ggatagccaa ccacgtcgcg ctggtcttta ttgaagtgat ccgcggcaca cctatcgtcg
    34861 tccaggtgat gtttatctat ttcgccctgc cgatggcgtt taacgactta cgcatcgacc
    34921 catttactgc ggcggtggtc accatcatga tcaactccgg cgcgtacatt gcggaaatca
    34981 cgcgtggtgc ggtgctgtct atccacaaag gttttcgtga agcaggactg gcgctcggtc
    35041 tttcacgttg ggaaaccatt cgctacgtca ttttaccgct ggcactgcgt cgtatgctgc
    35101 cgccgctggg taaccagtgg atcatcagca ttaaagacac ctcgctgttt attgttatcg
    35161 gcgtggcgga actgacccgt caggggcaag aaattattgc cggtaacttc cgcgcccttg
    35221 agatctggag cgccgtggcg gtgttctatc tgattattac cctggtgctg agctttattc
    35281 tgcgtcgtct ggaaagaagg atgaaaatcc tgtgattgaa tttaaaaacg tctccaagca
    35341 ctttggccca acccaggtgc tgcacaatat cgatttgaac attgcccagg gcgaagtcgt
    35401 ggtgattatc gggccgtccg gttccggtaa atcgaccctg ctgcgctgca tcaacaaact
    35461 ggaagaaatc acctccggcg atctgattgt cgatggcctg aaggtcaacg atccgaaagt
    35521 tgacgagcgc ctgattcgcc aggaagcagg tatggtgttc cagcagtttt acctcttccc
    35581 gcatctgacg gcgctgggaa acgtcatgtt tggtccgcta cgcgtgcgtg gcgcgaacaa
    35641 agaagaggcg gaaaaactgg cgcgtgagct gctggcgaaa gtcggtctgg cagaacgtgc
    35701 acatcactac ccttccgaac tttctggtgg tcaacagcag cgtgtggcga ttgcccgcgc
    35761 gctggcggtg aagccgaaaa tgatgctgtt tgatgaaccg acttccgctc ttgacccgga
    35821 actgcgccat gaagtgctga aggttatgca ggatctggct gaagaaggga tgacgatggt
    35881 gatcgtgacc cacgaaatcg gttttgccga gaaagtagct tcgcggctga tctttatcga
    35941 caaaggccgg attgcggaag atggcaatcc gcaggtgttg atcaagaacc cgccgagcca
    36001 gcgcttgcag gaatttttgc agcacgtctc ttaataagac acattgcctg atgcgctacg
    36061 cttatcaggc ctacaggata tctggcaact tattaaaatt gcatgaactt gtaggacgga
    36121 taaggcgttc acgccgcatc cggcaaaaaa gcccgcacgt tgtcagcaac ctgcttaata
    36181 tcccttcctc cctttcaccc gaaagggagg cacaccagat tcctctcatt taaaatcgcc
    36241 cctcccccag catctatact tatctttttg ctctgttttc tcactggagg agtcatgcgg
    36301 tggatcctgt tcatcctctt ctgcctgctg ggcgcacctg cccacgcggt atccataccc
    36361 ggcgttacaa ccacaacgac aacggactca acgactgaac cggccccgga accggatatc
    36421 gaacaaaaaa agcggcctat ggcgcactgg cggatgtgct ggataacgac acctcgcgta
    36481 aagagttgat cgaccagttg cgtactgtcg ccgcgacgcc ccctgcggaa ccggtcccga
    36541 agatcgtgcc gccgacgctg gttgaagagc aaaccgtgct gcaaaaggtc accgaagtca
    36601 gccgccatta tggtgaagcc ctttccgccc gcttcgggca actttatcgc aatatcaccg
    36661 gctccccgca taagccattt aatccacaaa ccttcagcaa tgcgctgagc catttttcaa
    36721 tgttagcggt attagtgttt ggtttttact ggctgattcg cctgtgcgca ctgccgctat
    36781 atcgcaaaat gggtcagtgg gcgaggcaaa aaaatcgtga gcgcagcaac tggttgcagc
    36841 ttccggcgat gattattggg gcgtttatta tcgacctgct gttactggcc ctgacattat
    36901 ttgtcggcca ggtattaagc gacaacctga atgcaggcag tcgcaccatc gctttccaac
    36961 aaagtttgtt tctcaacgcc tttgccctga ttgaattttt caaagccgta ctacgcctga
    37021 ttttttgccc aaacgtggcg gagctgcgcc cgttcacgat tcaggacgag agcgcccgtt
    37081 actggggtcg tcgcctgagc tggttaagca gcctgattgg ctatggcctg attgtggccg
    37141 tgccgattat ctctaatcag gtgaatgtac agataggtgc gctggcgaac gtcatcatta
    37201 tgctgtgcat gaccgtctgg gcgttgtacc tgatcttccg aaataaaaaa gagattaccc
    37261 agcatatgct caactttgcg gagcattcgc tggctttttt cagcctgttt atccgcgcct
    37321 ttgcgctggt gtggcactgg ctggcaagcg cctattttat catgctgttt ttcttttcgt
    37381 tgttcgatcc gggcaacagt ctgaaattta tgatgggtgc aacggtgcgc agcctggcga
    37441 ttattggtat cgcagcgttt gtttccggta tgttttcccg ctggctggcg aaaaccatca
    37501 ccctctcgcc acatactcag cgtaactatc cggagctaca aaaacggctg aatggctggc
    37561 tttcggcggc gctgaaaacg gcgcgtattc tgaccgtctg cgtggcagta atgttgctgt
    37621 tgagcgcatg gggattgttc gatttctgga actggctgca aaacggcgcg gggcagaaaa
    37681 ccgtagatat cctgatccgc atcgcactca ttcttttctt ctcggcggtt ggctggacgg
    37741 tgctcgccag tttgatcgaa aaccggctgg cttcggatat tcatggccgc ccgctaccca
    37801 gcgcccgtac gcgtaccctg ctgacgctgt tccgtaacgc gctggcggtg attatcagta
    37861 ccatcaccat catgattgtg ttgtcggaaa tcggcgtcaa tatcgcgcca ttgctggcag
    37921 gtgccggggc attaggtctg gctatctcgt ttggttcgca aacgctggta aaagatatta
    37981 tcaccggggt atttattcag tttgaaaacg gcatgaacac tggagatttg gtgactatcg
    38041 ggccgttgac cggcacagtg gaacggatgt cgattcgctc cgtgggcgtg cgccaggata
    38101 ccggggcgta tcacatcatt ccgtggtctt cgataaccac ctttgctaac ttcgtccgcg
    38161 gtattggttc ggtagtggca aactacgatg ttgatcgcca tgaagatgct gataaagcca
    38221 atcaggcact gaaagatgcg gtaacggaat tgatggaaaa cgaagaaatt cgcgggctga
    38281 ttattggtga accgaacttt gccgggattg tcggcttaag caataccgcg tttacactgc
    38341 gtgtttcgtt caccacgctg ccactcaaac agtggacggt acgctttgcc ctcgacagcc
    38401 aggtgaaaaa acatttcgac ctggcgggcg ttcgcgcgcc agtgcagact tatcaggtgc
    38461 tgcctgctcc gggcgcgacc ccggctgaac cgctgccgcc gggggagcca acgctttaac
    38521 gttggcgatt aacaaaacgg cggcgctgct cgtcgttcat aaaggtccag gcaataaagc
    38581 gactctgctt ttgcccctgg gccatctctt tttttaccac cttcaccgcg cccacgtcgg
    38641 tcagagcacg atacaacggc ggtaagtttt caccacgaga taccagcgag gtaaaccaca
    38701 tcacctgctt cgcgaagcct ttgctctctt caatcatctt tttgataaag ttgacttcac
    38761 cgccttcaca ccacaactcc tgttgctgac caccaaagtt cagtgcatcg tctttgttca
    38821 gccccaggtt acggcgttta cgctcactac ctgcccgcgc cgcagcggcg gaatcgtgga
    38881 atggcgggtt acacaaggtc gcgtcgtatt gctcgttttt gtggatgatg ccgttaaaaa
    38941 tcgccccact ctctttttgc cgacgcagac gaatggcgcg gttaagcccc ggattagcac
    39001 tgataatcgc ctgcgcactg cttaacgcct ggctgctggt ttcactaccg gtaaaacgcc
    39061 agccatattc atgtacgcca atcagcggat aaatacagtt cgcaccaacg ccgatatcca
    39121 gaatgctggc attcgctgga attgttccgc ttgcttcagc cagtaaatcg gcaaggtgat
    39181 gaatataatc cgcccggccc ggtactggtg ggcagagaaa accgtcgggg atatcccagt
    39241 tcgctacggc gtaaaaatgg gccagcaacg ccttattgag cgccttcacc gccagcggat
    39301 tggcaaagtc tacgctttgc tcccctgcgg gtgtaagcgt gaggaattgc ctgagttcag
    39361 gattgacctg acaaagcgtg gcgagatcgt agcggctgtg atgacggttg cgcggatgca
    39421 accccggttt ctgggcggac atagcattct cctttaagca gcggcgtaag atacccgcat
    39481 aacgcgacag ggtaaatatc tggctgcgtt tatcattccc caaaacctta acatggctat
    39541 ggtttatggc aattcatcat aaaaatcagc attatgcatg tcattgattt gtcttatttt
    39601 gtaaaaatat atcgttcaaa aaataagcat ttctctgtca attttttgcg tcatttgcat
    39661 cgcgcaggcg acctacactt atcaggcact acccgttgta tttatgagga tgtcattatg
    39721 aaaaagtgcc tcacactact gattgccacc gtcctgagcg gaatctctct cacggcttat
    39781 gccgcgcaac cgatgagtaa cctggacagc ggtcagctac ggcccgccgg taccgtttcg
    39841 gcaactggcg catcaaacct aagcgatctg gaggataaac tggcagaaaa agcgcgcgaa
    39901 caaggcgcga aaggttatgt cattaattcc gcaggcggaa atgaccagat gttaggtact
    39961 gcaaccatct acaaataacc ccaccaacgt aactgcattc gcctctggat gcagttttcg
    40021 cctgttttgt aatcaaaaaa catcaaatgt gaacgcaatg taaataaatg tatttctttt
    40081 tcgcgcaatg ggtgatagaa aatcgcatca agtgataatg cttatcaaaa ttattatcac
    40141 ttgcacgaac actatcacgg gattaacagt ggcatcgcat ccgcagagat gctttctcgt
    40201 ggcagtgaaa atttcaacat ataagaaaaa gtcacctgca aaatggaaaa caatcgcaat
    40261 ttccctgcca gacaatttca ttcgctcacg ttctttgccg gtctttgtat tggtattacg
    40321 cctgtggcac aggcactcgc cgccgaaggg caagctaacg cggatgacac gctggttgtc
    40381 gaagcatcaa cgccttcgct ttatgcgcca caacaatctg ccgatccgaa attctcgcgt
    40441 ccggtagcgg atactacccg cacgatgacg gtgatttctg aacaagtgat taaagatcag
    40501 ggcgcaacca accttaccga cgcgctcaaa aacgtccccg gcgtg
//
LOCUS       DAETQL010000032        38787 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.032, whole genome shotgun sequence.
ACCESSION   DAETQL010000032 DAETQL010000000
VERSION     DAETQL010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38787
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.032"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..247)
                     /gene="yefM"
                     /locus_tag="K0W65_001786"
     CDS             complement(<1..247)
                     /gene="yefM"
                     /locus_tag="K0W65_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBI3095073.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            530..571
                     /gene="hisL"
                     /locus_tag="K0W65_001787"
     CDS             530..571
                     /gene="hisL"
                     /locus_tag="K0W65_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBI3095074.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    547..670
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            717..1616
                     /gene="hisG"
                     /locus_tag="K0W65_001788"
     CDS             717..1616
                     /gene="hisG"
                     /locus_tag="K0W65_001788"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBI3095075.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1622..2926
                     /gene="hisD"
                     /locus_tag="K0W65_001789"
     CDS             1622..2926
                     /gene="hisD"
                     /locus_tag="K0W65_001789"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBI3095076.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2923..3993
                     /gene="hisC"
                     /locus_tag="K0W65_001790"
     CDS             2923..3993
                     /gene="hisC"
                     /locus_tag="K0W65_001790"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBI3095077.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3993..5060
                     /gene="hisB"
                     /locus_tag="K0W65_001791"
     CDS             3993..5060
                     /gene="hisB"
                     /locus_tag="K0W65_001791"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBI3095078.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5060..5650
                     /gene="hisH"
                     /locus_tag="K0W65_001792"
     CDS             5060..5650
                     /gene="hisH"
                     /locus_tag="K0W65_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBI3095079.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5650..6387
                     /gene="hisA"
                     /locus_tag="K0W65_001793"
     CDS             5650..6387
                     /gene="hisA"
                     /locus_tag="K0W65_001793"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBI3095080.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6369..7145
                     /gene="hisF"
                     /locus_tag="K0W65_001794"
     CDS             6369..7145
                     /gene="hisF"
                     /locus_tag="K0W65_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBI3095081.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7139..7750
                     /locus_tag="K0W65_001795"
     CDS             7139..7750
                     /locus_tag="K0W65_001795"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBI3095082.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="K0W65_001796"
     CDS             complement(7936..8913)
                     /gene="wzzB"
                     /locus_tag="K0W65_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBI3095083.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="K0W65_001797"
                     /pseudo
     CDS             complement(9059..10179)
                     /gene="ugd"
                     /locus_tag="K0W65_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="K0W65_001798"
     CDS             complement(10429..11835)
                     /gene="gndA"
                     /locus_tag="K0W65_001798"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBI3095084.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11944..12303)
                     /locus_tag="K0W65_001799"
     CDS             complement(11944..12303)
                     /locus_tag="K0W65_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095085.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12953..13516)
                     /locus_tag="K0W65_001800"
     CDS             complement(12953..13516)
                     /locus_tag="K0W65_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095086.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13723..14661)
                     /locus_tag="K0W65_001801"
                     /pseudo
     CDS             complement(13723..14661)
                     /locus_tag="K0W65_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14724..15098)
                     /locus_tag="K0W65_001802"
     CDS             complement(14724..15098)
                     /locus_tag="K0W65_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HBI3095087.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15102..16250)
                     /gene="wzy"
                     /locus_tag="K0W65_001803"
     CDS             complement(15102..16250)
                     /gene="wzy"
                     /locus_tag="K0W65_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HBI3095088.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16270..17175)
                     /gene="rfbG"
                     /locus_tag="K0W65_001804"
     CDS             complement(16270..17175)
                     /gene="rfbG"
                     /locus_tag="K0W65_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HBI3095089.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYIIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17168..18058)
                     /locus_tag="K0W65_001805"
     CDS             complement(17168..18058)
                     /locus_tag="K0W65_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HBI3095090.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18088..19344)
                     /gene="wzx"
                     /locus_tag="K0W65_001806"
     CDS             complement(18088..19344)
                     /gene="wzx"
                     /locus_tag="K0W65_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HBI3095091.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19360..19905)
                     /gene="rfbC"
                     /locus_tag="K0W65_001807"
     CDS             complement(19360..19905)
                     /gene="rfbC"
                     /locus_tag="K0W65_001807"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBI3095092.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19910..20788)
                     /gene="rfbA"
                     /locus_tag="K0W65_001808"
     CDS             complement(19910..20788)
                     /gene="rfbA"
                     /locus_tag="K0W65_001808"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBI3095093.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20847..21746)
                     /gene="rfbD"
                     /locus_tag="K0W65_001809"
     CDS             complement(20847..21746)
                     /gene="rfbD"
                     /locus_tag="K0W65_001809"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBI3095094.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21746..22831)
                     /gene="rfbB"
                     /locus_tag="K0W65_001810"
     CDS             complement(21746..22831)
                     /gene="rfbB"
                     /locus_tag="K0W65_001810"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBI3095095.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(23204..24097)
                     /gene="galF"
                     /locus_tag="K0W65_001811"
     CDS             complement(23204..24097)
                     /gene="galF"
                     /locus_tag="K0W65_001811"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HBI3095096.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24272..25666)
                     /gene="wcaM"
                     /locus_tag="K0W65_001812"
     CDS             complement(24272..25666)
                     /gene="wcaM"
                     /locus_tag="K0W65_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HBI3095097.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25677..26897)
                     /gene="wcaL"
                     /locus_tag="K0W65_001813"
     CDS             complement(25677..26897)
                     /gene="wcaL"
                     /locus_tag="K0W65_001813"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HBI3095098.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26894..28174)
                     /gene="wcaK"
                     /locus_tag="K0W65_001814"
     CDS             complement(26894..28174)
                     /gene="wcaK"
                     /locus_tag="K0W65_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HBI3095099.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28354..29832)
                     /gene="wzxC"
                     /locus_tag="K0W65_001815"
     CDS             complement(28354..29832)
                     /gene="wzxC"
                     /locus_tag="K0W65_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HBI3095100.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29834..31228)
                     /gene="wcaJ"
                     /locus_tag="K0W65_001816"
     CDS             complement(29834..31228)
                     /gene="wcaJ"
                     /locus_tag="K0W65_001816"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HBI3095101.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31283..32653)
                     /gene="cpsG"
                     /locus_tag="K0W65_001817"
                     /gene_synonym="manB"
     CDS             complement(31283..32653)
                     /gene="cpsG"
                     /locus_tag="K0W65_001817"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HBI3095102.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32846..34282)
                     /gene="cpsB"
                     /locus_tag="K0W65_001818"
     CDS             complement(32846..34282)
                     /gene="cpsB"
                     /locus_tag="K0W65_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBI3095103.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34285..35508)
                     /gene="wcaI"
                     /locus_tag="K0W65_001819"
     CDS             complement(34285..35508)
                     /gene="wcaI"
                     /locus_tag="K0W65_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HBI3095104.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35505..35984)
                     /gene="gmm"
                     /locus_tag="K0W65_001820"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35505..35984)
                     /gene="gmm"
                     /locus_tag="K0W65_001820"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBI3095105.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35984..36952)
                     /gene="fcl"
                     /locus_tag="K0W65_001821"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35984..36952)
                     /gene="fcl"
                     /locus_tag="K0W65_001821"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HBI3095106.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36955..38076)
                     /gene="gmd"
                     /locus_tag="K0W65_001822"
     CDS             complement(36955..38076)
                     /gene="gmd"
                     /locus_tag="K0W65_001822"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBI3095107.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38102..38650)
                     /gene="wcaF"
                     /locus_tag="K0W65_001823"
     CDS             complement(38102..38650)
                     /gene="wcaF"
                     /locus_tag="K0W65_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBI3095108.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(38666..>38787)
                     /locus_tag="K0W65_001824"
     CDS             complement(38666..>38787)
                     /locus_tag="K0W65_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBI3095109.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT        10355 a         9713 c         8729 g         9990 t
ORIGIN      
        1 caataatgtc cttttccgtt cctttgcctg atttcaggct atcgattgag tccatcaatc
       61 tccgggcgtt agcgggggaa cgcagtagat aagccgtctc ttccagcgaa ttgtattctt
      121 cgagtgacat cagaacacaa gcctctccat tctgacgagt aatgaggatc ggggcatgat
      181 cttcaacggc tttcatcatt gttgccgaca aattctgacg cgcttcgctg tagctaattg
      241 tacgcatgta aatctcctat tttgtacagt tcattgtaca atgataggtg ttaattaact
      301 atttattaat tagtttgtag atcaagacat tgccagcgag acgaaaaatc agactttgct
      361 ctttttggtg ctgtcaagtt agaggacagt cctcttagcc ccctcctttc cccgctcatt
      421 cattaaacaa atccatcgcc ataaaatata taaaaaagcc cttgctttct aacgtgaaag
      481 tggtttaggt taaaagacat cagttgaata aacattcaca gagattttta tgacacgcgt
      541 tcaatttaaa caccaccatc atcctgacta gtctttcagg cgatgtgtgc tggaagacat
      601 tcagatcttc cagtggtgca tgaacgcatg agaaagcccc cggaagatca ccttccgggg
      661 gcttttttat tgcgcggttg ttaacggttc agacaggttt aaagaggaat aagaaaatga
      721 cagacaacac tcgtttacgc atagctatgc agaaatccgg ccgtttaagt gatgactcac
      781 gcgaattact ggcgcgctgt ggcattaaaa tcaatcttca cacccagcgc ctgatcgcga
      841 tggcagaaaa catgccgatt gatattctgc gcgtgcgtga cgatgacatt ccctgtctgg
      901 taatggacgg cgtggtagac cttgggatta tcggcgaaaa cgtgctggaa gaagagctgc
      961 ttaaccgccg cgcccagggt gaagatccgc gctactttac cctgcgtcgt ctggatttcg
     1021 gcggctgccg cctttcgctg gcaacgccgg ttgatgaagc ctgggacggt ccgctctcct
     1081 taaacggtaa acgtatcgcc acctcttatc ctcacctgct caagcgttat ctcgaccaga
     1141 aaggtatctc ttttaaatcc tgcttactga acggttctgt cgaagttgcg ccgcgtgccg
     1201 ggctggcgga tgcgatttgc gatctggttt ccaccggtgc cacgctggaa gctaacggcc
     1261 tgcgcgaagt cgaagttatc taccgctcga aagcctgcct gatccagcgc gatggcgaaa
     1321 tggaagaatc taaacagcaa ctgatcgaca agctactgac ccgtattcag ggtgtgatcc
     1381 aggcgcgcga atcaaaatac atcatgatgc acgcaccgac cgaacgcctg gatgaagtca
     1441 tcgccctgct gccaggtgcc gaacgcccaa ccattctgcc gctggcgggt gatcaacagc
     1501 gcgtagcgat gcacatggtg agtagcgaaa ccctgttctg ggaaacgatg gaaaaactga
     1561 aagcactggg tgccagttca atcctggtcc tgccgattga gaagatgatg gagtgatcgc
     1621 catgagcttt aacacaatca ttgactggaa tagctgtact gcggagcaac aacgccagct
     1681 gttaatgcgc ccggcgatct ccgcttctga aagcattacc cgcaccgtta acgatattct
     1741 cgataacgtg aaaacgcgtg gcgatgaggc cctgcgtgaa tacagcgcga agtttgataa
     1801 aaccacggtt accgcgctga aggtgtctgc tgatgagatc gccgccgcca gcgaacgcct
     1861 gagcgacgag ctaaaacagg cgatggcggt ggcagtaaag agtattgaaa ccttccacac
     1921 tgcgcaaaaa ctgccgccgg tagatgtaga aacgcagcta ggcgtgcgtt gccagcaggt
     1981 cacgcgcccg gtggcttcag tgggtttgta tattccgggc ggctccgccc cgctcttctc
     2041 aacggtatta atgctggcaa ctccggcgcg tattgcgggc tgtaaaaaag tggtgctgtg
     2101 ctcaccgccg ccgattgccg atgaaattct ttatgcggcg cagctgtgcg atgtgcagga
     2161 cgtgtttaac gtcggcggcg cacaggccat tgccgcgctg gcgtttggta cggaatctgt
     2221 gccgaaagtg gacaaaatct tcgggccggg taacgccttt gtcaccgaag caaaacgcca
     2281 ggtaagccag cgtctggacg gtgcggcgat cgatatgccc gcaggcccgt cggaagtgct
     2341 ggtgattgct gacagcggcg ctacgccgga ttttgtggct tctgatttgc tttctcaggc
     2401 tgaacacggc cctgactcac aggtaatttt actgaccccc gatgctgata tggcgcgtcg
     2461 cgttgccgag gccgtcgaac gccaactggc agaactgccg cgtgccgaaa ccacccgcca
     2521 ggcactgaac gccagccgcc tgatcgtgac taaagattta gcgcagtgcg tggagatctc
     2581 caaccagtac ggcccggagc acctgatcat tcagacccgc aacgcccgcg aactggtcga
     2641 tggcatcacc agcgccggtt cggtatttct tggtgactgg tcaccggaat cggcaggcga
     2701 ctacgcctcc ggcaccaacc acgtgctgcc gacttacggt tacaccgcca cctgttccag
     2761 cctcgggctg gcagatttcc agaaacgcat gaccgtacag gaactgtcga aagaaggttt
     2821 ctccgcgctg gcttcgacca ttgaaacact ggccgccgcc gagcgcctga ccgcccacaa
     2881 aaatgccgtt actttgcgtg ttaacgtcct taaggagcaa gcatgagcac cgtgactatt
     2941 actgatttag cgcgtgaaaa cgtccgcaac ctgacaccgt atcagtcggc gcgtcgtctg
     3001 ggcggtaacg gcgacgtctg gctgaacgcc aacgaatatc ccacagccgt ggagtttaag
     3061 cttactcagc aaacgctcaa ccgctacccg gaatgccagc caaaagcggt gattgaaaat
     3121 tacgcgcaat atgcaggcgt aaaaccggaa caggtgctgg tcagccgtgg cgcggacgaa
     3181 ggtattgaac tgctgatccg cgctttttgc gaaccgggta aagacgccat cctctactgc
     3241 ccgccaacgt acggcatgta cagcgtcagc gctgaaacca ttggtgtcga atgccgcaca
     3301 gtgccgacgc tggaaaactg gcaactggac ttacagggca tttccgacaa gctggacggc
     3361 gtaaaagtgg tctatgtttg cagccccaac aaccccaccg ggcaactgat caatccgcag
     3421 gattttcgca ccctgctgga gctaacccgc ggtaaggcga ttgtggttgc cgatgaagct
     3481 tatatcgagt tttgcccgca ggcatcgtta gctggctggc tggcggaata tccgcacctg
     3541 gcgattttgc gtaccctttc gaaagccttc gcgttggcgg ggcttcgttg cggatttacg
     3601 ctggcaaacg aagaagtcat caacctgctg atgaaagtga tcccccccta cccgctctcg
     3661 acgccggttg ccgacattgc cgcacaggcg ttaagcccgc agggaatcgt cgccatgagc
     3721 gaacgggtgg cgcaaattat tacagaacgc gaatacctga ttgccgcact gaaagagatc
     3781 ccctgcgtgg agcaggtttt cgactctgaa accaactaca ttctggcgcg ctttaaagcc
     3841 tccagtgcag tgtttaaatc tttgtgggat cagggcatta tcttacgtga tcagaataaa
     3901 caaccctctt taagcggctg cctgcgaatt accgtcggaa cccgtgaaga aagccagcgc
     3961 gtcattgacg ccttacgtgc ggagcaagtt tgatgagtca gaagtatctt tttatcgatc
     4021 gcgatggaac cctgattagc gaaccgccga gtgattttca ggtggaccgt tttgacaaac
     4081 tcgcctttga accgggcgtg atcccggaat tgctgaagct gcaaaaagcg ggctacaagc
     4141 tggtgatgat cactaatcag gatggtctgg gaacacaaag tttcccgcaa gcggattttg
     4201 atggcccgca caacctgatg atgcagatct tcacctcgca aggcgtgcag tttgatgaag
     4261 tgctgatttg tccgcacctg cccgccgatg agtgcgactg ccgtaagccg aaagtaaaac
     4321 tggtggaggg ttatctggct gagcaagcga tggatcgtgc caacagttat gtaattggcg
     4381 atcgcgcgac cgacattcaa ctggcggaaa acatgggcat taatggttta cgctacgacc
     4441 gcgaaaccct gaactggcca atgattggcg agcaactcac taaacgagac cgttacgccc
     4501 atgtagtgcg caacaccaaa gagacgcaga ttgacgtcca ggtgtggctg gatcgcgaag
     4561 gtggcagcaa gattaacacc ggcgttggct tctttgatca catgctggat cagatcgcca
     4621 cccacggcgg tttccgcatg gaaatcaatg tcaaaggcga cctctatatc gacgatcacc
     4681 acaccgtcga agataccggc ctggcgctgg gcgaagcgtt aaaaattgcc ctcggcgaca
     4741 aacgcggtat ttgccgcttt ggttttgtgc tgccgatgga cgaatgcctt gcccgctgcg
     4801 cactggatat ctctggtcgc ccgcacctgg aatataaagc cgagtttacc taccagcgtg
     4861 tgggcgatct cagcactgaa atgatcgaac acttcttccg ttcgctctct tacaccatgg
     4921 gcgtgacact acacctgaaa accaaaggta aaaacgatca tcaccgtgta gaaagcctgt
     4981 tcaaagcctt tggtcgtacc ctgcgccagg ccatccgcgt cgaaggcgac accctgccct
     5041 cgtcgaaagg agtgctgtaa tgaacgtggt gatccttgat actggctgcg ccaacctgaa
     5101 ctctgtgaag tctgccattg cgcgccacgg ttatgaaccc aaagtcagcc gtgacccgga
     5161 cattgtgttg ctggccgata aactgttttt acccggcgtc ggcaccgcac aagcagcgat
     5221 ggatcaggtg cgtgagcgcg agctgtttga tctcatcaaa gcctgtaccc aaccggtgct
     5281 gggcatctgc ttagggatgc aactcctggg acgacgcagc gaagagagca atggcgtcga
     5341 cttgctgggc atcatcgacg aagacgtgcc gaaaatgacc gactttggtc tgccactgcc
     5401 acatatgggc tggaaccgcg tttatccgca ggcaggcaac cgcctgtttc aggggattga
     5461 agacggcgcg tacttttact ttgttcacag ctacgcaatg ccagtcaatc cgtggaccat
     5521 cgcccagtgt aattacggcg aaccgttcac cgcggcggta caaaaagata acttctacgg
     5581 cgtgcagttc cacccggagc gttctggtgc cgctggcgct aagttgctga aaaacttcct
     5641 ggagatgtga tgattattcc ggcattagat ttaatcgacg gcaccgtggt gcgtctccat
     5701 cagggcgatt acggcaaaca acgcgattac ggtaacgacc cgctgccgcg cttacaggat
     5761 tacgctgcgc aaggtgccga agtgttgcac ctggtggatc tgaccggggc aaaagatccg
     5821 gctaagcgtc aaatcccgct gattaaaacc ctggtcgcgg gcgttaacgt cccggtgcag
     5881 gttggcggcg gtgttcgtag tgaagaagac gtggcggcgt tactggaagc tggcgttgcg
     5941 cgcgtggtgg tcggttccac cgcagtgaaa tcaccggacg tagtgaaagg ctggtttgaa
     6001 cgtttcggtg ccgatgcgct ggtgctggca ctggatgtcc gtattgacga gcaaggcaac
     6061 aagcaagtgg cagtcagcgg ctggcaagag aactcgggcg tttcactgga acaactggtg
     6121 gaaacctatc tgcccgtcgg cctgaaacat gtgttgtgta ccgatatctc gcgcgacggc
     6181 acgctggcgg gatctaacgt ctctttatat gaagaagtgt gtgccagata tccgcaagtg
     6241 gcgtttcagt cctccggcgg cattggcgac attaacgatg ttgctgccct acgtggcact
     6301 ggcgtgcgcg gcgtaatagt tggtcgtgca ttactggaag gtaaattcac cgtgaaggag
     6361 gccatcgcat gctggcaaaa cgcataatcc cgtgtctcga cgttcgtgat ggtcaggtgg
     6421 tgaaaggcgt acagtttcgc aaccatgaaa tcattggcga tatcgtgcca ctggcaaaac
     6481 gctacgccga agaaggtgca gatgaactgg tgttctacga tatcaccgct tccagcgatg
     6541 gccgtgtggt agataaaagc tgggtatctc gcgtggcgga agtgatcgac attccgtttt
     6601 gtgtggcggg tgggattaag tctctggaag atgccgcgaa aattctttcc tttggcgcgg
     6661 ataaaatttc catcaactcc cctgcgctgg cagacccaac attaattact cgcctggccg
     6721 atcgctttgg cgtgcagtgt attgtggttg gtattgatac ctggtacgac gccgaaaccg
     6781 gtaaatatca tgtaaatcaa tataccggcg atgaaagccg cacccgcgtc actcaatggg
     6841 aaacgctcga ctgggtagag gaggtgcaaa aacgcggcgc cggagaaatc gtcctcaaca
     6901 tgatgaatca ggacggcgtg tgtaacggtt acgacctcaa gcagctgaaa aaagtgcgtg
     6961 aagtttgcca cgtcccgctg attgcctccg gtggcgcggg caccatggaa cacttcctgg
     7021 aagccttccg cgatgccgac gttgacggtg cgctggcagc ttccgtattc cacaaacaaa
     7081 taatcaatat tggcgaatta aaagcgtacc tggcaacaca gggcgtggag atcaggatat
     7141 gttaacagaa caacaacgtc gcgaactgga ctgggaaaaa accgacggtc tgatgccggt
     7201 gattgtgcaa cacgcggtat ctggcgaagt gttaatgctg ggctacatga acccggaagc
     7261 cttagacaaa accatcgaaa gcggtaaagt caccttcttc tcgcgcacta aacagcgact
     7321 gtggatcaaa ggcgaaacct cgggcaattt tctcaatgtg gtaagtattg ccccggactg
     7381 cgacaacgac acattactgg tgctggcgaa tcccatcggg ccaacctgcc acaaaggcac
     7441 cagtagctgc ttcggcaaca ccgctcacca gtggctgttc ctgtatcaac tggaacaact
     7501 gctcgccgaa cgtaaatatg ccgacccgga aacctcctac accgccaaac tgtatgccag
     7561 cggcactaaa cgcattgcgc agaaagtggg cgaagaaggc gtggaaaccg cactggcagc
     7621 aacggtacat gatcgctttg agctgaccaa cgaggcatct gacttgatgt atcacctgct
     7681 ggtgctgttg caggatcagg atctggattt aacgacagta attgagaacc tgcataaacg
     7741 gcatcagtga gttacgggct gagcaggtgt gatgttgttg tcacacccgg agccattgcc
     7801 cgatgcgcaa acttatcgtg cctacacatt atctcaacac acgtaggccg gataagatgc
     7861 gccagcatcg catccggcaa aaaaaacggg catggtgtca ccaccctgcc cgtttctctt
     7921 aaatgcacaa taatattact tcgcgttgta attacgcaga gcgttacgcc ccaacacaat
     7981 ccccgcgcca accatgccgc ccagaagcac tgccagaatc aaggtgattg cctttttcgg
     8041 actatcgcga cgaataggta acgtcggttt catcacatag cggtaagcat gaatatcaag
     8101 atcatcaaac tttaattttt caatatccaa caggttttga cgtgtctgat agtagtttgg
     8161 tgagaacacc aacggacgag tcgcttcatg cttaatcatc gactccagcg cttcgctccc
     8221 tagaaggaac aacgtatctt gcgtcacatc ttcagtctgc tgaacctgtg gctttgtcac
     8281 ctgcgcctga ttcgcatact gcaacgcttc ctgaatctga cggatacgca gatctttctg
     8341 ctcctgcgcg accacttcct gggttcttaa agagtcctgc aagtttttcc gtcccagagc
     8401 aatgttgtcc ttgagatcct tttctagctc ttgattcact ttatcatcaa cttgctgaat
     8461 gtattgggcc aacttcattt gtgcgccctc tgcagtttgc ccaacataag aaacagtcaa
     8521 tggtaattgc tggttcttaa cagaaggttc gatggtaagt ttttctggct cttcctgatt
     8581 atccagcgtt tctgctaatg cagagaaggc agaactgaag cgaccaatta acgtctcctg
     8641 caaatccgat actttcggtg cagcctgacc atagataaca ttcatggcat tgttatagcc
     8701 agcaatttgc cccacgtcgg gctgagtgat aattgctgtt gacgtccatt tctccttcgc
     8761 tactgccaaa taaccaatag ccagggcaat agccacaatg acggaaatga taattgtcat
     8821 cttgccacgc cacaactgca ctagtaaatc aatcaaatca atctgttccg ggtcatggtt
     8881 ttgcccagaa acattattat tttctactct catcattacc ctaactgacg gaaatttcca
     8941 agtccgcata gtttagcgat aatccccgtg aatgctatag gataagtgat aaaaataatg
     9001 aattattgcg gaggtaacaa aagacaatca gggcgtaaat agccctgata acaagatgtt
     9061 agtcgctgcc aaagagatcg cgggtgtaga ccttatctgc cacatcctta agctcttctg
     9121 ccatacggtt ggagataatg acgtcggctt gttgtttgaa ggtggcgaga tcacgttcca
     9181 ggcgagagtt gaagaatgag tcttctttca tcaccggctc gtagatgatc acttcaacgc
     9241 ctttcgcctt gatacgcttc ataatcccct gaatagaaga cgcacggaag ttatctgacc
     9301 cgctcttcat aatcagacga taaataccca ccacttgcgg tttgcatcga caattgccga
     9361 gatcaggtta ttcggcacag actggtagtt cgccagtaac tgcttggtat ctttcggcag
     9421 acaataacca ccataaccaa acgacggatt gttgtaatgg ttgccaatac gcggatcgag
     9481 acaaacgcct tcgataatct ggcgagtatt cagacctaaa ctttctgcat aactatccag
     9541 ttcattaaag tatgctacgc gcatcgccag ataggtatta gcgaaaagtt taatcgcttc
     9601 tgcttcagtg gagtcggtaa acagggtcgg gatattttgc ttaatcgcgc cttcctgtaa
     9661 caacgcagca aaacgttctg cgcgttctga acgctcaccg atgacaatac gtgacggatg
     9721 gagattatcg taaagggctt taccttcgcg gaggaattcc ggagagaaaa taatattttc
     9781 agtacgatat ttcttatgca tcgctgcggt aaaaccaacg ggaaccgttg atttgatgac
     9841 cataaccgca taaggattta tctcaactac gtctttaatt actgattcta cactggatgt
     9901 attgaaataa ttagttttag gatcatagtc ggttggagtg gcgatgatga cataatcagc
     9961 atcccggtag gcttcatttt tatctaatgt ggcattaaag tgtattttat ctgattgcaa
    10021 aaactgctga atttccttat caacaatagg agatatccga tcattcagca tagcaacgcg
    10081 tgacggtaaa atatctaatg ccacaacctc atgattttgt gcgattagaa gcccgtttga
    10141 caagcctaca tagccagtac cggaaatggt gattttcatt tcatgctctc agaattaact
    10201 taactgtgaa tcatgatgtt tttagcatcg tgataagatg ctaaaagttt taacgctacg
    10261 ttgtcagatg gtcaacgcaa cgaaaaacaa ttcagatttt ctttataaga atagctgatg
    10321 tttattaagt taatattaag cagtgaaatt tagtctataa gatatttggc aaaaaaaagc
    10381 ccggtgcaat atacgccggg cctcaatttt attgttggtt aaatcagatt aatccagcca
    10441 ttcagtatgg aacacacctt ctttatcaat gcgcttataa gtatgcgccc cgaaatagtc
    10501 acgctgtgcc tggatcaggt tcgcaggcag aaccgcggca cggtagctat cgtaataggc
    10561 aaccgcagcg gcgaaggtcg gaaccgggat accgttctgt actgcataag cgacgacatc
    10621 gcgcagcgcc tgctggtagt catcggcaat ttgcttgaag tacggggcca gcagcaggtt
    10681 agcgatctgc ggattttcgg cataagcatc ggtgattttc tgcaggaact gcgcacggat
    10741 gatgcagcca gcacggaaaa tcttcgcgat ttcgccgtag ttcagatccc aattgtattc
    10801 ttcagacgca gcacgcagct gagagaagcc ctgagcgtaa gaaacgattt tgcccagata
    10861 cagcgcactg cgaacttttt cgatgaactc agccttgtcg cctgctgact gagcttgcgg
    10921 accagagaga actttagatg cggcaacgcg ctgatctttc agagaagaga tataacgtgc
    10981 aaacacagac tcggtaatca gcgacagcgg ttcgccgaga tccagcgcgc tctggctggt
    11041 ccatttaccg gtgcctttgt ttgctgcttc atccagaatc acatcaacca ggtagttacc
    11101 gtcttcatct tttttggtga agatatcttt agtgatgtcg atcaggtagc tgctaagttc
    11161 accgttattc cactcggtga aagtctgggc cagttcttca ttagatagat tcaggccacc
    11221 tttgagcaga gaatacgctt cagcaatgag ctgcatatct ccgtattcaa taccattgtg
    11281 aaccatcttc acataatggc ctgcgccgtc ggcaccaata taagtaacgc atggctcgcc
    11341 gtcttctgcc acggcagcga ttttagtcag gataggcgca accagctcat aagcctcttt
    11401 ctgcccgcca ggcataatag aaggaccttt cagcgcgccc tcttcaccgc cggaaacacc
    11461 ggtaccgatg aagttaaagc cctctgctga aagctcacga ttacgacgaa tggtgtcctg
    11521 gaagaaggta ttaccaccat caatgatgat gtcaccttta tcgagatatg gtttcaggga
    11581 atcaatcgca gcatccgtgc ctgcacctgc tttcaccatt aacaggatgc gacgaggcgt
    11641 ttccagagat tcaacaaact ctttcaccgt atagtaagga gccagtttct tgcctggatt
    11701 ttcagcaatc acttcttccg tcttttcgcg ggagcggttg aaaatagaga cggtataacc
    11761 acgactttcg atgttgagcg caaggttgcg ccccatcact gccataccga ctacgccgat
    11821 ctgttgcttt gacattgttt actcctgtct tctatattat taaccgtcta ttaaccgtta
    11881 tattattaac cgtcgtgatt ttcatcttct aatcaaggaa tacatatggt tacagcatgc
    11941 ttcttacttt atagcgataa gtatgccatt gggtgtatcc ataagaactt tgaattttgg
    12001 atacttttct ggattaaaat tagcatagct tcgtttagat ataacaataa acgaactatt
    12061 aaaagatttc atcttctcta ttaaatcatc ctgtgggatt acctcacact cgggattact
    12121 aatagtccaa tcactatatc ttgctaatcc aaagacgtca ccaattgcca tccctttata
    12181 gaagtaaata ccatcctcca agcctaattg gataagatta tctccatata actcactata
    12241 ttcacttgct ttcttcatca gttcatagct atagcgactt tctaagcgtg aattaaaatt
    12301 caagtattca gaataaaaat atttgaattc aaaatacaac gatcgccccg aaaaaaaaat
    12361 aatacacaca aggataaaat atcgtcccaa cttgtaattt gtaatgttga tatcttttat
    12421 taaacttgca attgagatcg caacctgcaa tactgacaat gggtaaatgg gtgataaata
    12481 cctgtcacct tgcgtaacag aaaaccaaaa taagacatat gttaaaacaa tcgagaaaaa
    12541 tacccaaagt attttattat tcctaagtgt aaagacagac agtatagcat aaaaaacaac
    12601 atcactgcca actttattaa ttgcttgttg caaatctaat gtggcaggac ttataccatg
    12661 tcgagcttgt tctgctgttt gaacttgtag atcaatttca ttccataggt agtgaccaaa
    12721 tatatttcct ccagcaggac taaaaggatc accagagatc caaaaactac ggacatacca
    12781 ataaatgcca ccaagaattg ttgccaagct aaaaataaga aatgtctgta tgtttctctt
    12841 tttcgctatc aatataacaa atatgatggc tccatatatg gcaccaaata gttttacgcc
    12901 acaagcaatt ccaataaaaa aaccacataa aaaaattaat tcattttgtg ttttattgtt
    12961 ttctgagcga atatatataa gaatactggc tgcagcaaaa caaagatagc gttaaatcaa
    13021 tatatgcata accaagatga tctttaagtg gattgatagt aaatagtagg attgtagcaa
    13081 taatgcctgg ataataagtt ttcgtaattt cccatgaaca gccaacaagt ccacaggcta
    13141 ttacaaaaaa acagatattt gctaaaaact gagctaacct tacatctcct aactgtaaac
    13201 ctaaagccat taaaagatct gcattttgcg ggaacagagg aaaacgcaaa tattgttcaa
    13261 caacaattgc atggtgctca actaatgaac gagccaaagg taagtgatac atagtatcat
    13321 cccaatcacc gggagctcgc agggacgata acaatgtaac cacaaaaatc aaaacaagaa
    13381 ataaaataac aaacagcgta ctgaactcaa tattatttat tgccagccat ctaaattgct
    13441 gacgtttaat aatgcttaaa acccaacaca ccgtaaaaat taaaaagata gcaatcttat
    13501 taaattgccc gaccatgccc agaaagtaaa ttatcaaaat atttaaagac atccctatgc
    13561 ataggcaata aaaaataaag aaaatttgtt aagtcctgcc accccctcct taaaaaaaga
    13621 tgacaataca atattaccaa tgacaaaaaa tgtcattcca aaagcaaaac aatacacaac
    13681 atacaaatac aacggcaatt caatcatagt aaaatactct atttacatta tcagtttata
    13741 gtttttttca gaaagataaa ataatgcctt catttcacgt ctagttgttg tatttgaatg
    13801 caaaataatg ccacatacaa atgccattat tgacgataac atgatggagg cagttaaaaa
    13861 ggcagtcgga aatcttctca ccaaacctgt atcaatataa tcaacaatga tgggcaatcc
    13921 taaaattata gacaaagcag caagtaaaac gccgataata ctaaaaaagt atagtggccg
    13981 ttcaagcgag taaagtttaa ttattgtttt cagaatcttg aacccatcac tccaagttga
    14041 tagtttgctc acactccctt cagatcgctc gccatatttt gtattaacct caccatactt
    14101 catacgtaat tctaaagcat gaattgtaag ctcagtttcg atttcaaatc cccttgatag
    14161 acatggaaaa gatttaacat agcgtctgga aaatacacga taccccgaca acatatcagt
    14221 aaaattccct ttaaagatag tctgaacagt accagtcaac aatttatttc ctcagcgatg
    14281 cccttttctg tatgtatttt gatctccatt ctcttgacga catcctacaa ccatatccaa
    14341 ttgatttttt atgagatggt taatcatttc tggagcagaa cttgcatcat aagtgtcatc
    14401 tccatcaacc attagataaa tatccgcatc tacatctgag aacatacgtc ttacgacatt
    14461 ccccttgcct ttcagaggaa cagagtgaac tgttgctcca gcatcctctg ccacttgtgc
    14521 tgtactgtca gtcgaattat tatcaaatac atatatagaa gcagttggaa tattcgtttt
    14581 gaaatcattt atgacttttg ctattgcctt ctgctcatta taacaaggaa taattacagc
    14641 gatattaaaa gagtgataca taagcccctc atataaacta tttagctaat gtgcaagtaa
    14701 ttgtgttttt aacaaatcgt ttactaacga aaaatgaaat attttgatgc gcaatatcct
    14761 aaaaccaaac taaaaagaga aaaggtgaac aatgtaaata ttggagataa agctaagaga
    14821 tcaaataaaa cgccgaataa ataactcatc gcccccataa aaaatataaa tataaaatat
    14881 ctagccttgg ttgcagatac tttaaatgag catcttgcat ttgcaaaaaa agaaaaggtt
    14941 gctgcacata taaaaccaac aatgttactt cggccttgac caaaagctaa attattataa
    15001 cacgcataaa atacccccca atgtaaagca gtattgacaa ttccaattaa aaaataacta
    15061 aaaaaagaac tggtcaataa cttccctatt tttaacatcc tttattttgc tccagaagtg
    15121 aggttattac taatttggat attttctata gaaaataccc ataaaactac aacgcctata
    15181 ataaaaattc ccatgggtgt aaaaagaaat ggattagtta tagaaacggt aatactgaaa
    15241 aaaagtatta acgacatcat taatgatgtt tttgtactat ttaaaaaata gcctttcaaa
    15301 aagataagca acataggaac taatgatata aatacaccaa taacgcctgt tttctgaagt
    15361 atctcaagag gtactatttc aatccttaat cgatctaata taaatgaacc aaatcctctt
    15421 ccaaacaaga acgtcgctaa atcaacattt ttataataaa aatataaatc attaaatcta
    15481 acagagtcag aatcttcact gcgtgatccg accatgtaaa gcataattat aaatagaacg
    15541 ctgcccaata ttattataat agtatttaaa tatagcttat gtaatttaaa actggctaat
    15601 aacagtgata atgttgtaaa aagtaataca cctcttgatt ctgtcaataa tatcgcaagc
    15661 actattatga gacattttaa atattttttg tcgacaaaac tgataatcgc accgacacca
    15721 aaaaagtaga aggatttgga aaaaaaagcc ccatcgggac gaaaaaagaa ctcgctagtc
    15781 aatgataagt gttcataaat taaattaaaa tttaacaaac cgaaattcaa caaaattatg
    15841 tatgaaatat ataacagtga tccatataat gcaaatgcat taactatttt aataaaaatc
    15901 tcacatgaaa atacttttaa agtatatctt agtgtaaata gatatataag tgacagcata
    15961 aaaaggtatg gttgcacatc aaacagtata tcatttattt tattaccagc taataagccg
    16021 acaaagaagc cataaaaagg aaataaaagc aaccatataa aaagactgac acatttttgg
    16081 gtgattgaaa aattgaatgt aactaaatta aaaattaaaa gtataaaact aaatacaaat
    16141 aataaatatc gcaaagggaa tattcccaat ggctccagta atcttccacc accaccaata
    16201 atcagttcaa tacataaaaa tgttataaaa attttattta tattattcat attcgtaagg
    16261 tgatgttttt taaaatatgt ttaaaatcaa cattaataaa atataaattt tatcagcctt
    16321 tttccgcctt aacttccaaa aacttaaagc caagatcaaa ttgcctatat aacctttacc
    16381 caattgatta ttcacaaagc tatcatcagc taaggcatta gctattttag ttacttcttt
    16441 tcgataccac ttgtttctca ccaaaccaag ccgtttgtat tttgctttaa aactaatgtt
    16501 tgctcccact tggttgtttt catgctgtct gtacagcatt gttggttttc tatcaatata
    16561 ccacgaataa tttctcgtcc gcgcaaatga atataaaaac caatcatgca gacagatatc
    16621 ttgtgcagca tttttattat ttatgatgaa cttcttaaat tcaataagcg tttctttttt
    16681 tataatatat gtacaccccg gacctgctgc ttcaaaaaaa taatcgaatt gagtttgcga
    16741 ctgagcctta tcaactaaat tttttcgccc tgatggataa tatgcaatta cattgctgga
    16801 ataacaattc gcattattaa ataccaagca gtcaattgca ttttttattt tatctttata
    16861 ccaaatatcg tcctgatcac taaaacagat ataatcatag ttttcaagat cgacatcttt
    16921 aattaatcta taaaaatttt tacctgcccc cccaaactta ttgacgcttg gaagtatatt
    16981 tataattgag ggatatgata attgaaattc ttcacaaata tttaatgtat tatcagttga
    17041 taaatcatct gatataaaaa ttgagatgtc tacatctttt tgttcaataa tactttttaa
    17101 ttgttcctca atccagcatt cgccattata cgtagccata agtacagcta ctcttttttg
    17161 atcagaacta tgcattataa cgaccgcccc cagtaattcc tcttattcca tccagcgtag
    17221 ccaaaatatg ttttttcata tattcaattt tatttttttg agttgcgata attaaagtcg
    17281 tatgaattaa agaaaaaaca acttccctaa tagagagtaa aagaggcaca tgattttttc
    17341 taagcaaaag aaatgcattt cttactcgat aataacgacg tactggcgag tgaattggta
    17401 tgttaatccc acagattttt actgacttat ccccaatagc atgaaccatt tctatatcag
    17461 gaatgatatg aactaatatg ccatagttta agcatctaag acaccattct gtatcgacat
    17521 aatcaataaa aagttcctca tccatcattc caacaatctc aaaaacttct tttgaaacca
    17581 tggttccgga tgagattgtt actgaacttt taaacggtgt ctgtccttca gtgacatgaa
    17641 ttttctctcg aagaccattt tttttgatat tacaaatagg ataataatgt gacttatctc
    17701 tatcaataaa aactgggcca gctatttttg cattttcatt atccatatag caggaaagtt
    17761 ttttgataaa ctcattgcga atacggctat cttgatcaaa aaaaattatc ttctctgccc
    17821 ctttttcaag agaatatcga atcccaatat tttgtgcttt tgctatacct tcattcctac
    17881 caagacaaat taaatgaact ttttcaatgt ctgctaattt atctttcaag gtgaaattag
    17941 tcctattatc gactactaca acgtaacaat tttgctcctt tagttcagtg atcagtgcat
    18001 tcagattttt aagttcggga ttatatgtca caatgacagc gtaaatatta ctattcatta
    18061 tccaagtgac tcagtaattg gttaatttta gttttcatat acagaacaca ttctagtata
    18121 aagccattgt aataacagag catctaaaat tacacgtatg ctccaggata ttgcggcacc
    18181 cagtaaccca tatttttcga ccataaaata caaaagggct aaataaggaa ccaattcagc
    18241 acaatgaatc aaagcagtaa ttttagattt tcctaatgcc tggattgcag agaatggtat
    18301 cattgccagc gcattgaaga aaaaaccgat caataacaca tttaatatat taaaggaatt
    18361 aatttgataa ttctcaccaa accatatttt caatacaagc cctgaatata acaagcctat
    18421 gattatcacc ggcaaacaga ttagaaacat taaaagcaat gatttattta cattacgttt
    18481 aaattcttta aagtctttga tgttacttaa ccttggaaac actgccctcc cgattgctgc
    18541 aggtattatt cctaatttta gaataacctc cgctggtgca gaataaaatg caactttatc
    18601 tgcccccata atatttgaca ctataaatcg atcaaaatac accatgaccg gacttatgat
    18661 attacttact gtcatccaac cgccaaagaa aaagagtcgt ttaaaggttt tataacaaaa
    18721 atgaaccccc gaaattttaa tatcattcct gacataatag gcagagacta aaatcgaaat
    18781 aactcttgca aatattaaac cagcaaccgc tgccgacaat gtagcactat aaaaaacaaa
    18841 tatggccgga attcccgcga tgcaagagct tgatatagat ttttgaatat ttacaatgcc
    18901 aaatttttca tccccctcaa gaatggctga ccataattga tttagaataa ataatggaat
    18961 gcaaatagcc aacagtttaa atgctagttg tatatcacta tgttcaacac cagaaatttt
    19021 tagataatta acaattccat cagagaaaat caataataaa aaagcaccaa agcatgaaaa
    19081 tagcaccaaa aaagatgtac ttgttgaaat taccttggtt ctttcatgat gattatcacg
    19141 atgaattgca atttctctaa taacagagcg agtcaggcct acatcaaaaa ttccagcata
    19201 cccaacaagt gcaattgcaa ttgtataaac accgaaacct tcaggcccca aacctcgagc
    19261 gagaaatcct agcgagggga tagcaattaa agttggtatt gcatagccaa aaaggttcca
    19321 gacactattt tttattatgc tcattattac atttctttat catataaaat aatcagcatc
    19381 tacaaatttt tgaccattaa tatctttatc agaaagcaat ggattttgaa ccggccattc
    19441 tatatttata cttttgtcgc tccataaaat cccacgctct tgcatttttg aatagaaatt
    19501 atttgttttg tagacaaatt ctgcttcatc actaagaacg caaaacccat gagcgaatcc
    19561 ttccggtatc cataattgac gcttattctc cgccgacaaa tttacaccaa cccacttgcc
    19621 aaatgttggt gatgatttcc gaatatcaac agctacgtca aaaacttcac cgacaacaca
    19681 gcgtaccagt tttccttgag cataaggttc cagctgatag tgcagtccgc gtaacacacc
    19741 cttagttgat ttagaatggt tatcctgaac aaactcaacc tttcgtccta cagcctcttc
    19801 gaaaactttc tgattgaagc tttccataaa aaagccccgc tcatcaccaa atacttttgg
    19861 ttcaaaaatt agcacatcag gaatttcagt tttaattacg ttcattttat taataacctt
    19921 taatcatttt cagcagatac tgaccataag catttttctt cagcggctcg gctaatacct
    19981 tcacttgctc agcatcaata aagcctttac ggtgagcaat ttcttccggg caggaaactt
    20041 tcagcccctg acgctcttca attgttgcaa tgaagttgct tgcttcaata agactttgat
    20101 gtgtccccgt gtccagccat gcataaccac gccccatcat agcgacagac aaacgtcctt
    20161 ggtccatata aatacggtta atatcggtaa tttccagttc gccacgtgca gatggtttaa
    20221 ggtttttcgc catttctaca acgtcattgt catagaaata aagcccagta accgcatagt
    20281 tactttttgg ttcctgcggt ttttcttcca gactaattgc cgtaccgtta tcatcaaatt
    20341 ccaccacacc gtagcgctca ggatcattta cgtgataggc aaaaaccgtt gcgccactct
    20401 ctctgttaac agcggtatcc attaatttcg gcaggtcgtg accatagaag atattatcac
    20461 caagtaccag cgcacaatca tcaccaccta taaattcttc accaatgata aatgcttgcg
    20521 ccagaccatc cggactcggt tgtactttat attgcagatt aagcccccac tggcttccat
    20581 caccaagcaa ttgttgaaaa cgtggtgtat cctgcggcgt actgataatc agaatatctc
    20641 gaatacccgc caacataagc gttgaaagcg gataataaat catcggttta tcataaatcg
    20701 gtagcagttg tttgctcaca gccattgtca caggataaag acgagtgccg gaaccaccag
    20761 ccagaataat acctttacgc gttttcattt caccattcct tttaattcat ctcgctctag
    20821 catcatgagc gagatgcaat aatttgttaa attgccgtgg tcgtaaataa ttcgttgagc
    20881 atacgtttca cgcccacctg ccagtcaggc aagacaagcg caaagttctg ctgaaatttt
    20941 tcggtattga ggcgagaatt atggggacga cgtgctggtg tgggataggc cgttgtcggc
    21001 acggcattaa gtttgttaag agcaaggttg atccccgctc tgcgcgcttc ttcaaatacc
    21061 agagcggcat aatcgtgcca ggttgttgta ccaccagcaa ccagatggta caaaccagca
    21121 acttctggtt tatttgcggc cacgcgaatg gcatgagcgg tgcaatcagc cagtaattca
    21181 gcccccgttg gtgcgccgaa ttgatcgttt attacagcca actcttcgcg ttcttttgcc
    21241 aggcgcaaca tcgttttggc aaagttattt cctttaccag cgtataccca gctggtacgg
    21301 aaaataagat gctttgcgca atgttcttgt aacgcttttt ccccagctag cttggtttca
    21361 ccgtagacat ttagcggtgc tgttgcatcc gtttccagcc atggcgtgtc accgttgcct
    21421 gggaaaacat aatcagtgga gtagtgtata acccaggctc cgacttcatt agctgctttt
    21481 gcaatcgctt cgacgcatgt cgcgttaagt aattgcgcga aattcggttc tgattccgct
    21541 ttgtctactg cagtgtgagc agcagcatta acaataacat cggggcgaat ttttttgacg
    21601 gtttcagcca caccttcggg gttgctgaaa tctccacaat aatcagtgga gtgaacatcg
    21661 agcgcaatca agttacccag aggagccaga gcacgctgca gttcccaacc cacctgccct
    21721 gttttgccga aaagcaggat attcattact gacggccctc atagttttgt tcaatccacg
    21781 actgataagt accgcttttc acattctcaa cccaatttgt attagccaga taccattcca
    21841 ccgttttacg aatcccgctc tcaaacgttt cttgcggttt ccagcccaat tcgcggctaa
    21901 ttttatcggc atcaatggcg taacggcggt catgccccgg gcgatcggca acataagtaa
    21961 tttgctcacg ataagatttc tctttcggta caatctcatc caacaaatca caaatagtga
    22021 gcactacgtc gatgtttttc ttttcgttat gtccaccaat gttataagtt tcacccgctt
    22081 taccttcggt tacgacggta tataacgcac gagcatgatc ctctacatac aaccagtcgc
    22141 gaatttgatc ccctttacca taaataggta atgccttacc ttccagagca ttcagaataa
    22201 ccaatggaat aagtttttcc gggaagtgat aaggaccata attgttcgag caattagtca
    22261 caatggttgg taaaccataa gtccgcttcc aggcacgtac caaatgatcg ctggaagctt
    22321 tagacgcaga atacgggcta cttggcgcgt aagctgtcgt ttccgtaaat agcggtaacg
    22381 cttcgttatt atttgcttca tccggatggg gtaagtcacc ataaacttca tcagtagaaa
    22441 tatgatgaaa gcggaaactt tttttctttt catcattcag agcagaccag taattgcgcg
    22501 ccgcttctaa aagaacataa gtccccacaa tattggtttc aataaatgcc gcagggcccg
    22561 ttattgaacg gtcaacgtgg ctctctgccg ccaggtgcat caccgcgtct ggctggtgct
    22621 gtgcgaaaat acgcgacatg gctacagcat cgcagatatc tgcatgctca aaagcataac
    22681 gttcagaatc agaaacatca gcgagtgatt ccaggttccc ggcgtatgtt aatttatcga
    22741 cattaacaac actatcttgc gtattattta ttatgtgacg aacaacagca gaaccaataa
    22801 accctgcgcc accagtaaca agtatcttca cttttttatt ccatatagct atagagcatg
    22861 ctgtgaaata gactgctcca gatttgatta atagaggtat taatacacgc taccgcccct
    22921 ggcttaacag ctaccagtgc actgcatgca tctcaacgat gtggcgagcg ttagccactc
    22981 gcgccaaatc cgaaaaattc aaacgttaat tgtcttacca aacagccgta gaaacaagga
    23041 aaatcctgga aaaatttgaa taatatgagc tatgtaactt cttgttttta taggctttat
    23101 aaaaacggca acggcaggaa aattcaatga gcaaaactta tgccacgaat cttcactgcc
    23161 gttataattt tcttatcaac cgttacatcc ggtcagattt tcattattcg cttaacagct
    23221 tctcaatacc tttacggaac ttcgcccctt ctttcaggtt gcgtaggcca tacttcacaa
    23281 acgcctgcat atagcccatt tttttgccgc agtcgtaact gtcgccggtc atcagcattg
    23341 catcaacgga ttgttttttc gccagctcgg caatagcatc agtcagctga atacgtcccc
    23401 atgcaccagg ctgagtacgt tccagttccg gccaaatatc ggcagaaagc acatagcgac
    23461 ctacggccat gatgtctgag tccagcgtct gcggctgatc cggtttttcg ataaattcaa
    23521 caatgcggct gactttaccc tcacggtcca gcggctcttt agtctggatg acggagtatt
    23581 cagagaggtc acccggcata cgttttgcca gcacctggct gcggcccgtt tcgttgaaac
    23641 gtgcaatcat ggcagcaagg ttgtaacgta gcgggtcggc gctggcatcg tcgatcacaa
    23701 cgtctggcag taccacgaca aatgggttgt caccaatggc aggtcgcgca cacaaaatgg
    23761 agtggcctaa acctaaaggt tcgccctgac gcacgttcat aatgatcacg cccggcggac
    23821 agatggactg tacttccgcc agcagttgac gcttcacgcg ctgctcaagg agtgattcta
    23881 actcataaga ggtgtcgaag tggttttcga ccgcgttctt ggacgcgtga gttaccagga
    23941 ggatttcttt gatccctgca gccacaatct cgtcaacaat gtactgaatc attggcttgt
    24001 cgacgattgg tagcatctct ttgggtatcg ccttagtggc aggcaacata tgcatcccga
    24061 gacccgctac aggaataact gcttttaaat tcgtcattat ttcatccacc tgtaaaatgg
    24121 ttgctgaatt atagcttgtt cgattttttt cgccagcatc aattaccctg aattgattac
    24181 tgaattactt gtgatgttac gccgcttcgt tgtggattgc agtagcattg ttcctaagta
    24241 tggctccatt tttccaggaa tggtcgcaaa tctactccct ccgttccggc aatctaaagt
    24301 taatcttttc cacattaaca atatggtgat taatcctgtc gatatcgacg gagctttgcc
    24361 ctctttcatt caccgcatga acatttgcaa gagacagcag tgtttctttt ttcgccataa
    24421 agacgccgcg aacgtctttg cgcatgtcga agttcatgct caatgctggt ccaactgagg
    24481 attcctgcat cacattgata tttcgcataa aaagatgttg cggcttgttg tgtaactcca
    24541 gcgaggcacg cttcatctca atgttagtta gcgacacaaa ggagacggca ttcccggcgg
    24601 agatttggat gccgcgcaat ttataagcaa gatgggtatt atccagttga atattattca
    24661 ctcggaaatt ttgcggtatc gagagatatt tgcctttaat taccccatag ccgattaaca
    24721 tcccggcgct attaatcatt tcaatattat caatcacgaa attgtcacaa ccgtaaatag
    24781 cgactgtcgc gttatcaatg cccgctttct tactgaaatc cggcgtgata ttgcgggcat
    24841 tgatattacg aataacaaaa tgtttaccat tttcaacatg gatcaactgc cgacaatccg
    24901 atcccgtgat attcgccacg acaaagtttt tcactgcctg gtcttccggg tagttgttat
    24961 cataagtgct tcccgcaagg cctatgccga tgccccagtt gattttgccg ttggtacagt
    25021 tgatacgctc gatgacatgg tcagatatca aaatatcact gtcgttaatt gccacgttcc
    25081 attcaatggc gtcgccctgt aagtcgctga acttacagtt ggtaatgttg gcaccgataa
    25141 tctggttatg aaatccctgg cgtaagatgg cgtaattagc gtggctgaca gtgaggttat
    25201 cgatggtcag gttgcgcatg acccgtttgt ttttgccgcc aatataaatc tgcattaccg
    25261 ggccaaagcc gctcatcgcc agccctttga tggtgcagtc agaaccacgc acatccaggg
    25321 taatgttatg cataccgccg ccctctcccc ctgtcacctg gctgccgtcc tgtaagacaa
    25381 atcgccctct gccgttgccg cgcaggcttc caaggatgtg taacgttttg ccgggaggga
    25441 tgaagatgcc ggtgttgata ttgtcacaaa cgaatccggc aggcacgacg accgtttgcc
    25501 cttcgctgaa ggcttgttta aatgaggcga tccagtcatg tgggttgtag tcgttaatgt
    25561 taacgctttg tcgggcggga agtgcgcgag cgaaaggggt atggaggaag gcaagcgccg
    25621 agcttgccgt caggaaggtg cgtcgggaga gtgttttaaa tggcatacgt tctcctctat
    25681 aaagcctgca gcaagctggc gagttctcga ttaatcacct gctggttaaa atcgtgttcg
    25741 actttttcgc acgcacgttt gacgaccgga gccagttcgt cggtatccag ttggctaaac
    25801 gccgccagac gttgtgccag tgcgcgagca tcgttctcag gcagcagcca gccggattta
    25861 tcggcctcca ccagttcagg aattccgcta tgcagagtag aaaccaccgg aataccgacc
    25921 gccatcgctt ccattagcgc caccggaatg ccttccatat cgccatccgc gcccgttacc
    25981 gatggcaaca ggaagacatc cgcgtcgtcg agcatctcct tcacttcatg gctcggttta
    26041 aagcccggca tctctaccac atcttccagt tgatattgtt cgatgatggt gcgcaggcgt
    26101 cgttcccaca ggccaatgcc gaggatgcga tagcgaaatg ccacgccctg ctctttcaac
    26161 tgacggcagg cttcaatcgc cacatgcagg ccttttttct cggttaagcg tgcgacagat
    26221 atgatttcca gcggcgttgc gggcgctttc accggacgcg ggctaaagcg cgtcatgtcc
    26281 acgcccatac gcgatacggc gattttttct ctggggcagc ccattttttg cagccttccg
    26341 gcccacagat cgcttatcgg taacatcagg tcgccacggc gaaacagttg ttgatattcg
    26401 ggagtgtagt ggttgagcac ttcccgactg gagatatcaa taccgtggaa gatggtggca
    26461 attttgccgc gaatgatacc cagttcgcgt agttttgctg cggttacccc cgccgggcca
    26521 aaatgagcga tgaacacatc ggcgcgaaac ggtgttgcga cctgaccaca aatggcagac
    26581 aaaatcaggt tccgcgactc ggcaccatag cgtttgaggt tgagcgcatg ccaggtattt
    26641 ttacgatgaa tgccacgtaa ggtctggctg gcgcggtggc gcagtttcgc tactttgccc
    26701 gcaggttcgt cctgtaacca gcgggttctg gcgacaaggt tgtatttcgt ccatgccgcg
    26761 tgggtgttct ctgtatcgcc tttttgcaaa gcgacaatct ccacttcaaa gcccatatca
    26821 ataaacgcgg taatttgatt gaggacgaag gtttccgacg acagcgggaa tttcaataag
    26881 aagaagccga ccttcatttc acctccccga tgcgctcaag cacagactgc accatctgca
    26941 ttcccgtctg acgttcgcga ctgacggctt cgttaaatcg agcgttcagc accggaagct
    27001 ggcctaaggt atccgcaacc atcgcttgca ggctgccgtc cagtaaatga cgaatatcaa
    27061 tcgccatctc tggcagtccc agctgctgca taatcccggc ggatttatgt tcatagttga
    27121 tggcaatcgc cggagtagca aagttcattg agataatggc ggagtgcagg cgcgtaccga
    27181 cggtgagttc acaggctccg agaattttgc ccatttccag atcgttgagt tcatccatca
    27241 ctacgtggta acgggcagga tcgctgatgt gctggcgcag attgagcgcc accatgcggt
    27301 cgtctttgtt atagctgtcg atacccgtac aggtggagag cgcaatcacc tgatagcctt
    27361 catcgagaat gcgattgacc accccggcaa aggctttttc atacgcttgt tgagtggtgc
    27421 cgagacgttt gtcgaacggt gccagttcgc gcagggtaat tgccactgtt ttctgttgtg
    27481 cggcaacgtc cagccagtgt tgaacggcat agctggcggt gaagtcttcg gtgtggtgat
    27541 cgaccagcca cgcggtatcg acgccatgtt ccacttttgc ggtggtgata tttctgcgtt
    27601 tcatcaaatc aaggctgacc gattcgcgca ggatcagcgc gtcgcagtga ccaaagacat
    27661 agttcgccag ttggttaaat tgctcatcct ggaaggggcc gacgctgtga ccaatcataa
    27721 acagcggctt tttcgccata aatgtgcaaa gcgcatgttc aaactgcggc acgccgtaga
    27781 gatcgacaaa aaacgatccg ccgacctgaa taatggcgtc gtaacctgac agcaggcgca
    27841 cgaagtcggt gaatccctgg gcgatggcga tattgcgcag cttgccagtg tcggtgacgc
    27901 gtgagagcaa tacctgatgc tggtagcggc gacggaggac ttttttaatg cgcccgacaa
    27961 cgcccgccgc gctattgtgt tgtgtcatct gcaggaacag cggatcgccc attaccgggc
    28021 ggttgagcag ccaggaagaa ctgaccggat agcggctcat cacgtccact tcggtatgtg
    28081 gattgaggat gttgatggca tcaagtaagc cgcgcaagat ggcgctgtcg ccacgattgc
    28141 cgcaagtgtg gttgcccaga ataagtaatt tcattttttc ctcataaatt tgatgccagg
    28201 tgaggctgtg tttgttgtgt tcgttgccgt tttttgcctg atgcgacgct ggcgcgtctt
    28261 atcaggccta caggtcccga gcacagaacc gtaggacgga taaggcgttt tacgccgcat
    28321 ccggcaaccg ttgtcggaac cgaaaacagc aattcacccc gcccgtaaaa gcattttcat
    28381 tttttcgctg cgacaaaact gacgcttcac ttccaccacc agcggatggc gtgacagcac
    28441 aatcatcacc acaaacgcca gcacgcccgc ggctatttgc accgccagca gcatccccag
    28501 cgccagttgc cctttcagca caatgcccag cgcataactg accaccagcg tcggcaacga
    28561 gagataaaac ggcagccaca aactcaggat gtactggcga taactggaac caagaaccgg
    28621 tttaatcatc acgaagtaac tcagaatggt gttgataatc tgcaccagca ggaaaccgag
    28681 cgtgacgccg attgcgcccg ccatctgccc accaataaca atcgccggaa taaacagaaa
    28741 tgttttgaat acgttgaatt taaagctgat atcgacccgc gctttcgcca tcagcagcga
    28801 accaatcggg ttacccacgg agcgcagcag acccacaata cacagcaatt gcagcaccgg
    28861 aataatgctg ttccactttt cgccgaatac cagcggtacg aagttattcg acaccaccat
    28921 cagccccagc agcgccggaa agttgataat ccccactatc gacagcagct tgtagaagtt
    28981 aacacgcagc ttttcggtgt cgtcctgaat tttggcaaac gccggaaaca acacgcgggt
    29041 gatgattggg ttcagcttca tcggcggtac gacggccacg ttgtacgcca ggttgtatcc
    29101 ccctgccacg cccgcgccaa gaatacgcgc cagcaccagc gttgaaaggt tggtgttgag
    29161 atagttgatg atgctgtccg ccgtcagcca ggcaccaaaa cgtaagttcg gtgccacaga
    29221 cgccagcgag aagtgcagac cggggcaata aattttgcgt ccaaagtagc caaacagcag
    29281 tgttctcacc gcactattga ccagataacc gaggatcgcg gtcatcgcca gcggccagaa
    29341 atgggcgcta actaccgtaa aagtgaagcc cgccagcacc gcgctggttt cgatcatgcc
    29401 gattttgttg aactccagct ccttctgcat caacgcgcgg aactgttgcc cgtgggggat
    29461 caccacaaac gccagcgaca atgttttaat caacggtgcc aggtccgggt tattcagcac
    29521 gtcgccgatg acatcactca acaaaaacac cgccacacat accacaagcc ccagcccgac
    29581 gttcagccag tacagcgtgg tgagttcaag gtgactgatt tcttttcgct gaataatcga
    29641 gttagcgata ccgaagtcag aaagcgtatc tgccagcgcg ataatcacca gcgacacagt
    29701 cagcaggccg aactggtggt tgtcgataat ccgcgccagc acggtcatct gcaccagccc
    29761 gaggccgatg atgatcaccg tggcaatagc cgaccacttc gcgccgctga tggttttttc
    29821 acgtaagctc atatcaatat gccgctttgt taacgaagcc cttgaatacc gtcaggaaaa
    29881 cgattttgat atcgaaccag acgctccatt cgcggatgta ctcaaggtcg aactcgacgc
    29941 gtttttccat tttctccagc gtgtcggttt cgccgcgcca gccattaatc tgcgcccagc
    30001 cggtaatgcc cggtttcact ttatggcgca gcatgtagcc ttcaatgagc tggcgatact
    30061 gttcgttatg cgctactgcg tgcggacgtg gaccgacaat cgacatcccc ccggtcagca
    30121 cattgataaa ctgcggcaat tcgtccagcg aggtgcggcg cagaaagttc cccactttag
    30181 tgacgcgcgg atcgttctgc gtcgcctggg tcaccacttt gtcgttctcc atcactttca
    30241 tggaacggaa cttccacact ttgatcggct tgccatccat accatagcga gtctggcgga
    30301 aaataaccgg ccctggtgaa ctgagtttca ccgccagcgc aatacagcac agcaccgggg
    30361 agatcagcag cagaataagc gtcgccagca caatgtcttc cgcacgtttt agcaggcggt
    30421 taacccccga aagcggcgtg tcatacagcg gcaccaccgg aacgccgttc atctcttcga
    30481 gacgtgaatg gagaatgttg aaggtaaaga cgtcggggat cagcagcacc gaacaggtgg
    30541 tatccgccag ttgatggacc agttttttca ctcgtgcgcc gtcgcacatt tgcatcgcga
    30601 tatagacgtt atgaatcttg cccgcttttg catcctcgac cagctgttgc aggttacccg
    30661 cccagtcgtt agaaacgccg cccggtttcg ggtcgtggta aacgcccacc acttcaaacc
    30721 ctaaccacgg ctgattacgg aaactctcca tcagcatttg tccggcggct aaatcccccg
    30781 ctacggcgac catccgcttg ttgtagccat gattacgcag ccagcccgcc ccaatgcgaa
    30841 tacatgaacg gcaaaccacc agtccgatgc tggtcagccc ataccacgcc agccagattt
    30901 tcagttgcgt gtcgaaatca ttgttgaacg ccaccagtcc ggcgctgaaa atcacgctta
    30961 aggtccagtt ttgtagcaac agggcaaatt ctgtcgctgc ccgaacaccg cgccatgagc
    31021 gataaaaatc ggtgatgccg cccagcatct ggaacaccac cagcgtaatc agcgccacca
    31081 acaggtgcat gtagaggaat gacagtccgc tgacttcgca aaccagccat agcccggcaa
    31141 acatgatggt gatatctgaa aagcgttgca ccatagagat taacgatgca ttggttttcg
    31201 ctcgctcgcg cttttttaga tttgtcatcg ttgttcctgt tattagcccc ttacccgcag
    31261 tggggtaagg gaagatccga cattactcgt tcagcaacgt cagcagagtt cgcgttcgcg
    31321 cttccatcag cggcacatca ccgcgcgatt ccacattcag gcgcaccacc ggttcggtat
    31381 tggaggtgcg caggttaaag cgccagtcgg caaaggtcat gctgatgcca tcgctgcgat
    31441 ccaccgccag cgcctcacgg ctaaaatgct gttccacgcg gttaatcgcc tcaacgggtt
    31501 gcgccagttt gctgttgatc tcaccgcttg ccggaaacgc cgccatccgg tcgcgcacca
    31561 gttcgcccag cgtttttcct ttcaggcaca ccagttcggc gaccagcagc cacgggatca
    31621 tgccgctgtc gcagtaagcg aaatcacgga agtaatggtg ggcgctcatt tcgccaccgt
    31681 agatggcgtc ttccttgcgc atccgttctt taataaaggc gtgtccggtt ttcgacatta
    31741 ccggcgtgcc gcctgcagca gtcaccacat caatagtgtt ccaagagaga cgcggatcat
    31801 ggatgatctt cgcgccggga tttttttcga ggaacgcttc tgccagcagg ccgacaatgt
    31861 agtagccctc gataaactgc cctttttcgt caaacagaaa acagcggtca aaatcgccat
    31921 caaaggcaat gcccatatcc gcgccgtgtt tgatgaccgc attgcgggtg tcgtcgcgac
    31981 attccggcag cagcgggtta ggaataccgt tggggaaatt gccgtccggt gtgttgtgta
    32041 ctttgattaa ttccacgggc gcgccgaggg ctttaaagcg ggcttcaatg gcgtccacca
    32101 ccggacccgc tgcgccattc ccggagttga tcaccagctt gagcggcgtg agatttttga
    32161 cattgatata accgaacagg tgatcaacgt aagcgtcacg caggttgatt tgctgatagc
    32221 gaccgcgttt ggtttcatcg acgggaggaa agtcgttggc ttcagccaga cgctggacgt
    32281 cgcgcagtcc ggtatcaccg ctgatcgtgc gagccccctc gcgcaccagc ttcatgccgt
    32341 tgtaatccat cggattatgg ctggcagtaa cttcaatgcc gccatccacg ccgagatgga
    32401 acgtggcgaa atagatctct tcggtgccgg acataccgat atccagcaca tcgacgcccg
    32461 catcctgtaa accttttgcc agcgccagtt ttaaggtttc gctggtgaga cggacatcac
    32521 cgcctaacac aatggttttc ggtttgagaa attcgccata ggcgcgacca atgcgccagg
    32581 cgatatcttc attcagttct tcgcctaatt tcccgcgaat atcataggct ttaaagcagg
    32641 ttaatttttt catgtcgtta ccctttttca ggcaatagtt ggccctgacc gaaacggcca
    32701 attttatttt tgtcgttatt tacaccgcgg tttcgcattc attgcctgat gcgacgctgc
    32761 cgcgtcttat caggcctaca aaaccgaacc gtaggtcgga taaggcgttc acgccgcatc
    32821 cgacaaacag cgcctgatgc gacgtttaca cccgtccgta gcgatccgcg aagcgcacca
    32881 catcatcctc ttcgagataa gagccggagc gcacttcaat taaatcgagc ggaattttcc
    32941 ccgggttttc caggcagtgc gtcgccccca gcggaatata aatggactcg ttttcaccaa
    33001 gcagtttgat atcaccatca atggtgactt ttgccgttcc cgcgaccact acccagtgtt
    33061 ccgcgcggtg atggtgcatc tgtaccgaca aaccctcgcc cggtttcacg gtgatgcgtt
    33121 tcacctggta gcggtcgccc gcgtcgatag agtcatattt gccccacgga cgatacactt
    33181 cgcgatgcac ccgatgctca tggcgaccat cggctttgat ctgctcgacc acttttttca
    33241 catcctgtac cgcgttacgg tcggcaatca gcaccgcatc tttggtctgc accactacca
    33301 gatctttcac cccgacggtg gtgaccaggc ctgattcggc atacacatag ctgttttcag
    33361 ttttgtgatt aatcacatcg ccgtggcaaa cgttgccctc ggcggtgtgg gcgctgatct
    33421 cccataatga agaccaggaa ccaacatcgc tccagcccgc atccatcggc accacaacag
    33481 catctgccgt aggttccatg accgcgtaat ccaccgactc ttccgggcag gcgagaaacg
    33541 cttcttcatc cacacgaata aaatcgagat ccggatcgac ggcgctcatc gctttttcgc
    33601 aggcatcgag gatatccggg cgatattttt tcagttcttc gagatagcgt ccggcgcgga
    33661 acaggaacat gccgctgttc cagtaatatt cgccgcttgc tacataggcc tgggcggttt
    33721 ccagattcgg tttttcgaca aactgcgcca cttcaaaggc caccgtatcc tgctcaccca
    33781 cagacacttc accgcgacga atatagccat aaccggtttc tggtagatcc ggcacaatgc
    33841 caaaggtcac cagcttgccc gcttcggcat aaggcatggc attacgcacg gcggcacgga
    33901 acgcgtcttc atcggcaatc acatgatccg ccgccaatac cagcattaac gggtcgctct
    33961 ccgggctatg acgttttgcc gccagcgccg ccagtgcaat ggcaggtgca gtgttacgcc
    34021 ctgccggttc gagaataatg ttctcggtga gtttgttcag ttgacgcagc tgttccgcga
    34081 caataaagcg gtgctgctca ttgcaaatca ccaccgggct ttcgcactcc acgccgttca
    34141 ggcggcagat ggtggtttgc agcatggtga gatcgccttt caggcataaa aactgcttgg
    34201 ggtaaagtac gcgggaaagc ggccataaac ggctaccgga gccacctgcc atcacaactg
    34261 gatagagttt cgactgcgcc ataattatcc ccgaatatca tttataaatt gacgtaacac
    34321 gttctcttta tcgagcgtgc gttcggcata ttcacgtgcc accgtgttat gtttgggcag
    34381 caggagcgcc tgacgaatcc ccgccaccag cgcctcgacc gattccggtt ctacgcaaac
    34441 cgcaataccc ggaaaggttt cgcaaagctg ccccagttct gtgtgggcct ctgcggtaat
    34501 caccgcgtta ccgcctaccg ccagaatatt ggtcagtttc gacggcaata cggcatctgc
    34561 cgcgccgcgt ttttgcacca ccagatggca atcgcccatc ttcagcagtg caggtaaagc
    34621 gtcatacgat tgcagcggga aaaattgcat gttgcgcagt ccacgctgct gcgccatttt
    34681 ttccagccgc gctttgccgc cgccctgccc gacaatggca aaaatcagcg gttcatcgcg
    34741 caggagatcg gcagcttcaa taacgttttc cagcccctgc ttttcaccaa tattgccgga
    34801 gtaaagaatg atttttttgt tatccggcag gccaagctgg ttacgaaggg catcaacatc
    34861 ggcgtctgca acatgctgaa aacgggcaat ttccgaccag ttggggaaga agatgacgtt
    34921 atccgccgcc acgccttttt cgatggcttt attcatcatc gaacgcgaaa tcgtggaaac
    34981 gttatcgacg ttatgcagtc cgctacgttc gaacgccgtt gccagctgtg ccactttgcc
    35041 gcctttgcct tttccggcaa ggcccagccc cagcatggcg tccacttcgt aatcctgaat
    35101 atgcagcacg gtacgcgcac cagagagctt cgccagcagg cgcattcccg gcgtgcaaaa
    35161 cagcgttggc actacgccaa taatgcgatc cggcttccag cgacgttgcg ccatcagtgg
    35221 aaagaaactg ctgacggcaa aactgccgag atgcaacaag cgtttcaggg tgctcggctg
    35281 ttttggcacg tacagcgggc agcgccacac cgtggcggcc ccttcttctc gtttgtagcg
    35341 ccaggcggaa tagttctcac ccacctgcca ttgcgggtag taaggcggtg cggtaatgac
    35401 ccgcacctca tgaccttgtg ccgccaacca ttccaccatc tcgccggtgt atttgccgat
    35461 gccggttaac tccggcgagt agttaatgcc gtagaccagt attttcataa tccgggtact
    35521 ccggcacgct tctcagcgag aaaataggct cggctgttag cgtgaacatc gttactggcg
    35581 agcagcgcgt ccggcgtcag ccagcggtaa tcgtcatgct gctcatccgg cagtaacagc
    35641 tcttcttccg ctactctgaa gcgaaaaccg agcaccacat agtgagtggt gaaatccgtg
    35701 ccggagaagt tatcgtcata aaaatgctgc cagacaccgt aaaactggcc tgctgttatc
    35761 ggcagacgca gccccagttc cgccatcgtc agccgctcaa atgcggcttc cagcgtttcg
    35821 tctttctgca cgcgccctcc cggcacaaac cagtaaccct gcgccgggcg gttggttctt
    35881 ttgccaagca gaaactcgcc gcgactgttc tcgacaataa agtcgagaga gacaagcggt
    35941 gtggagcgca ctacagtggc aaagtcttcc tgacgtaaaa acatcattcc ccccgaaagc
    36001 ggtcttgatt ctcaaggaac cactggtaag tgctggcaag ccccgcttcc agtgagattt
    36061 cgtgatacca gccaagctga tgcaggcgcg tcacatccag cagtttgcgc ggcgtaccat
    36121 ccggtttgct ggcatcaaaa accacccgac ctttgtaacc caccactttg gcgatggttt
    36181 gcgccagctc gcggatagtg cagtcaacgc ccgtgccgac gttaatgtgc gacagcatcg
    36241 gttgggtatt ctccagccag acttcatgcg ccagctccat gacatgaatg ctcgccgccg
    36301 ccatatcatc gacgtgcagg aattcacgca tcggtgtacc gctgccccat accaccacgt
    36361 ccggcgcatt ctgtgccgtc gcctcgtgga agcgacgcag caatgctggt atcacatggg
    36421 agttactcgg gtggaagttg tcatgcggcc cgtacaggtt ggtcggcatg acagtgcggt
    36481 aatcgcgtcc atactggcgg ttgtaagatt cgcacagttt gatcccggcg attttggcaa
    36541 tggcgtaagg ctcgttggtc ggctccagcg tgccctgcaa taactcgctt tctgccatcg
    36601 gctgttttgc cagtttcggg tagatgcagg acgatccgag aaacatcagt ttgttcacgt
    36661 cgttctgatg cgcggcgtga atgatgttgc tctcaatcat catgttctgg tagatgaaat
    36721 ccgccggata ggtgttgttg gcaacaatgc cgcccacttt cgccgccgcc agatagacct
    36781 ggtcaatgcg ttcgctggca aagaaatcat gcacggcgcg gctgtccagc aggttcagtt
    36841 catcgcgggt acgtaatacc agctccacat caccgcgctg ttcgagctgc cgcttgatgg
    36901 cagaaccgac catcccacaa tggccagcga taaaaatgcg ttgtttactc atgcttatga
    36961 ctccagcgcg atcgccacgt cgtagccgtg agatttcagc agagagtgtt ttttcgccgc
    37021 ttcgaggtca ttagccacca tttcagacac catctctctg agggtgattt ccggtttcca
    37081 gcccagtttt tcgtgcgctt tggtcgggtc gccgagcagc gtttcaactt ctgccggacg
    37141 gaagtaacgc ggatcaacgg cgataatcac atcacccggt ttaacgcccg gcgcgtcatg
    37201 cccggtgacg gaaaccacaa tgcccttctc ttcaacaccc gtgccttcaa agcgcagttt
    37261 gatgcccagc tgtgctgccg ccatttccac aaactgacgt acggagtact gaacgccggt
    37321 cgcgataacg aaatcttccg gctgttcctg ttgcagcatc atccactgca tttttacgta
    37381 gtctttggcg tggccccagt cacgcaggga atccatattg ccgaggtaca ggcacgactc
    37441 cagcccctgg gcgatgttgg cgattgcgcg ggtgattttg cgggtaacga aggtttcgcc
    37501 gcggcgcggg gattcatggt tgaagagaat tccgttacaa gcgtacatgc cgtaggattc
    37561 gcggtagtta acggtgatcc agtaggcgta cagtttggcg accgcatacg gagatcgcgg
    37621 gtagaacggc gtggtttctt tctgcggaat ttcctgcacc agaccataca gttcagaggt
    37681 ggaagcctga tagaaacgag ttttcttttc cagaccgagg aagcggatcg cctccagcag
    37741 gcgcagcgta cccatcgcgt cgacgtcagc ggtatattct ggtgactcaa aagagaccgc
    37801 aacgtggctc attgcgccta ggttgtacac ttcatccggc tgtacttcac gcaaaatgcg
    37861 cgtcaggtta gaggtatcac tcaggtcgcc ataatgcaga tggaatttcg ggttgcaggt
    37921 gtgcggatcc tgataaatgt gatccacgcg ctcggtgttg aatgacgatg cgcgacgctt
    37981 aataccatgc acctcgtaac ctttttccag cagaaactct gccaggtaag aaccgtcttg
    38041 tccggttaca ccggtgatga gagcgacttt tgacatgtct tattcctctg tatttttgaa
    38101 tttattcagt ttcaacgcgt tcgcgtatca ccactgcggg attcccccgg caaaccacat
    38161 ttgccggaag cgatttaaaa acactgcttc gtgcacccac gacggtgccg tcgccgattg
    38221 tgacgcctgg ggcaacaaag acatcggttg ccagccagca tttctcgcca atcacaatag
    38281 gcgtggcgtt aatggtgaaa tgttgacttg catggtcgtg gctaccggtg cataaataac
    38341 ttttttgcga tatcaccgaa tgtgcgccaa tggttatttc accgagggta tataaattga
    38401 cgtcatcgcc aacccacgcg taatcaccta aggttaattt ccacggatag gtaattttta
    38461 ctgacggacg aataactacg ttttttccta tttttgctcc gaataaacgt aataaaaaag
    38521 cccgccagcg atacaatact tgtggcgacc aggcaaatat tgttgcctgt actgcccacc
    38581 ataattgcac tttaatagcg ttgccgcccc ggaacccttt cggcaccgag aaaccgctta
    38641 aatcttgcat tggttttcct tatattcaga ctttgttata taaggcttta gtcttacctg
    38701 tcgtacgttg gcgtaaatgc caggataatt ctgcccagaa gccaggtaca tgtaatatct
    38761 gccgttggac ttttttggcg tcagcac
//
LOCUS       DAETQL010000033        37628 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.033, whole genome shotgun sequence.
ACCESSION   DAETQL010000033 DAETQL010000000
VERSION     DAETQL010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.033"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..176)
                     /locus_tag="K0W65_001825"
     CDS             complement(<1..176)
                     /locus_tag="K0W65_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HBI3095110.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="K0W65_001826"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="K0W65_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBI3095111.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="K0W65_001827"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="K0W65_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBI3095112.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="K0W65_001828"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="K0W65_001828"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBI3095113.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="K0W65_001829"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="K0W65_001829"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBI3095114.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="K0W65_001830"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="K0W65_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBI3095115.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="K0W65_001831"
     CDS             complement(5180..5422)
                     /locus_tag="K0W65_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBI3095116.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="K0W65_001832"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="K0W65_001832"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBI3095117.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="K0W65_001833"
                     /gene_synonym="fabE"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="K0W65_001833"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBI3095118.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="K0W65_001834"
     CDS             complement(8339..9313)
                     /locus_tag="K0W65_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBI3095119.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="K0W65_001835"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="K0W65_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBI3095120.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQILIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="K0W65_001836"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="K0W65_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HBI3095121.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="K0W65_001837"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="K0W65_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBI3095122.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="K0W65_001838"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="K0W65_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBI3095123.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="K0W65_001839"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="K0W65_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBI3095124.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="K0W65_001840"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="K0W65_001840"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBI3095125.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="K0W65_001841"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="K0W65_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HBI3095126.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="K0W65_001842"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="K0W65_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBI3095127.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="K0W65_001843"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="K0W65_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HBI3095128.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /locus_tag="K0W65_001844"
     CDS             23180..23383
                     /locus_tag="K0W65_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBI3095129.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="K0W65_001845"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="K0W65_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBI3095130.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="K0W65_001846"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="K0W65_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBI3095131.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="K0W65_001847"
     CDS             26388..26660
                     /locus_tag="K0W65_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HBI3095132.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="K0W65_001848"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="K0W65_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBI3095133.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="K0W65_001849"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="K0W65_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBI3095134.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="K0W65_001850"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="K0W65_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBI3095135.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="K0W65_001851"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="K0W65_001851"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBI3095136.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="K0W65_001852"
                     /pseudo
     CDS             complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="K0W65_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31907..32305)
                     /locus_tag="K0W65_001853"
     CDS             complement(31907..32305)
                     /locus_tag="K0W65_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBI3095137.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32499..33626
                     /gene="zapE"
                     /locus_tag="K0W65_001854"
     CDS             32499..33626
                     /gene="zapE"
                     /locus_tag="K0W65_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBI3095138.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33845..34273
                     /gene="rplM"
                     /locus_tag="K0W65_001855"
     CDS             33845..34273
                     /gene="rplM"
                     /locus_tag="K0W65_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBI3095139.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34289..34681
                     /gene="rpsI"
                     /locus_tag="K0W65_001856"
     CDS             34289..34681
                     /gene="rpsI"
                     /locus_tag="K0W65_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBI3095140.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35076..35714
                     /gene="sspA"
                     /locus_tag="K0W65_001857"
     CDS             35076..35714
                     /gene="sspA"
                     /locus_tag="K0W65_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBI3095141.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35720..36217
                     /gene="sspB"
                     /locus_tag="K0W65_001858"
     CDS             35720..36217
                     /gene="sspB"
                     /locus_tag="K0W65_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBI3095142.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="K0W65_001859"
     CDS             complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="K0W65_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBI3095143.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMPFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9116 a         9413 c         9900 g         9199 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatgcc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaactggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatctg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagagt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgggcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgttcg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctca ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag atcctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gcctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 cagacaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacctggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ctggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag gacaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca ctaattattg
    27061 aactaattaa taccacacta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctgggcaaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggccg
    30661 acgaagaggt gctggtatca agcgtcactt ctacttccag tggtttgccg ttacgcagca
    30721 ggccaagctt cactttcgtg cccggctctg tagtcgcgat acgagagcgc aactcagcaa
    30781 agctattcag cggtttgccg ttgaggctgg taataatatc gcccgctttg acgcccgctt
    30841 tcgctgagcc agaacttggc aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt
    30901 tgaaggcttt ggcgatatcg gcactcatct cggtgccttt gatgcctaac aaaccgcgtt
    30961 tgatttcacc aaagtcgata agctgctgcg ccagtgttcg cgccatatta ctggggatgg
    31021 caaatccaat cccgacgctc ccgccgccag gcgcaaggat tgcagtgttg atgccaatta
    31081 actcaccgtt aaggtttaat agtgcaccgc cggagttacc gcggttaatg gaagcatctg
    31141 tctggataaa gttttccaga ccttcaagat tcaacccgct gcggcctaat gcggaaacaa
    31201 tgccagaggt ggcggtttgc ccaaggccaa atgggttacc gaccgctacg gcaaaatcac
    31261 cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt taatttgctc gggttttgaa
    31321 tttataacag ggcgatatcg ctctggtcat cgctaccaat cagttttgca tcaaactcgc
    31381 gcccatcatt gagctgaata ctgattttct gtgcctgatt aatcacatgg ttgttggtca
    31441 gcacatagcc tttactggcg ttgatgatga caccggaacc taaaccttcg aagggttgtg
    31501 caggttgatc cggtaaatca tcaccaaaaa actttttgaa ttcttccggg attttctgtc
    31561 cctgactggc cgttccttcc acccgtacgc tcaccactgc cggaagcact ttttccagca
    31621 ttggagccag actggggaga ggggcctgat cggcaacctg gcctggaatc gacgcgacgg
    31681 cctgaaatga cgccgagaga gttaacccgg cacttaacgc taatgcactc aacagctggg
    31741 tttgtttttt cattattcct gctctcgtac ctgaatgata agaaaagaga ttcaaaacag
    31801 tttgatgtta ttgaattttc agacggctaa caatgagaca ccagattaaa tgatagtcgg
    31861 ggagggagag aagaggaggg cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg
    31921 cgccagtacg cagcaggccg gatgcgcctt cagaatagtc gcgaggcacc tgcaccggtg
    31981 cctgatcgtt gctggcttca gactctgcca gacgattacg gaacgggttt gcttcagcag
    32041 acagttccgg cagcaggctg ctggagcttt ttgccatgtg ctgatacagc tggcgatagt
    32101 cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc
    32161 gatactcgtc cagttcagct ttattctttt ccagttcgta ctgcaacgcc tgttgctggc
    32221 gtagtttacg attaccaaaa cgcatggcca cagcaccaat aatgatgccg acgactaacc
    32281 caattagcgc atattcccag gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg
    32341 ttggcttcag gctccatgcc tgcggctgat tatgccacta taaccgttaa ttccacagaa
    32401 gtggaatccc gactgcatat cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac
    32461 aacggcgcac cgattattca aggaataaca ataagatcat gcaaagcgtt accccaacat
    32521 cgcaatacct gaaggcgctc aatgaaggca gccatcaacc cgacgacgtt caaaaagagg
    32581 ccgtcagccg cctggaaatt atttatcaag aactcatcaa tagcaggcca ccagccccca
    32641 ggacgagtgg gctaatggcg cgggtcggta agctgtgggg taaacgcgaa gacacaaagc
    32701 atacgccagt gcgtggctta tatatgtggg gcggtgtagg acgcgggaaa acctggctga
    32761 tggacctttt ctatcaaagc ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt
    32821 ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg gcagaccgat ccgctggaaa
    32881 ttattgccga tcgctttaaa gccgaaactg acgtgctctg ttttgacgaa ttttttgttt
    32941 ctgatattac cgacgccatg ctacttggcg gtctgatgaa agccctgttc acccgcggta
    33001 ttaccctggt agcgacgtca aatattccgc cggacgaact ttatcgaaat ggcctgcaac
    33061 gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg
    33121 acgctggtgt tgattatcga ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac
    33181 tcaacgatga aacccgggcg cagatggata aactatggtt ggcgctggcg ggggtgaaac
    33241 gagaaaattc accgacgtta gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga
    33301 accagacgct ggcggtttct tttactacgc tgtgcgtcga cgcccgcagt cagcatgact
    33361 atattgcgct ctcacgcctg tttcacacag taatgttgtt tgatgtgcca gtcatgacgc
    33421 ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc
    33481 atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga aatttatcag ggtgagcggc
    33541 tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc
    33601 tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat cacaaaaagg ggtcgatctt
    33661 tgaccccgac ttctctataa tcctgcgacc ccacgttaca agaaagtttt tttcccaaaa
    33721 ctttttgtgt gctggcatag gctattcgaa ggggtaggtt tgccggactt tgtcgtgtga
    33781 acctcaacaa ttgaagacgt ttgggtgttc accaacgtgt aactatttat tgggtaagct
    33841 tttaatgaaa acttttacag ctaaaccaga aaccgtaaaa cgcgactggt atgttgttga
    33901 cgcgaccggt aaaactctgg gccgtctggc tactgaactg gctcgtcgcc tgcgcggtaa
    33961 gcacaaagcg gaatatactc cgcacgtaga taccggtgat tacatcatcg ttctgaacgc
    34021 tgacaaagtt gctgtaaccg gcaacaagcg tactgacaaa gtgtactatc accacaccgg
    34081 ccacatcggt ggtatcaaac aagcgacctt tgaagagatg attgctcgcc gtcctgagcg
    34141 tgtgattgaa atcgcggtta aaggcatgtt gccaaaaggc ccgctgggtc gtgctatgtt
    34201 ccgtaaactg aaagtttacg cgggtaacga gcacaaccac gcggcacagc aaccgcaagt
    34261 tcttgacatc taatcgggat tataggcaat ggctgaaaat caatactacg gcactggtcg
    34321 ccgcaaaagt tccgcagctc gcgttttcat caaaccgggc aacggtaaaa tcgtaatcaa
    34381 ccaacgttct ctggaacagt acttcggtcg tgaaactgcc cgcatggtag ttcgtcagcc
    34441 gctggaactg gtcgacatgg ttgagaaact ggacctgtac atcaccgtta aaggtggtgg
    34501 tatctctggt caggctggtg cgatccgtca cggtatcacc cgcgctctga tggaatacga
    34561 cgagtccctg cgttctgaac tgcgtaaagc tggcttcgtt actcgtgacg ctcgtcaggt
    34621 tgaacgtaag aaagtcggtc tgcgtaaagc acgtcgtcgt ccgcagttct ccaaacgtta
    34681 attggcttct gctacggcag aaaacaattt gcgaaaaaac ccgcttcggc gggttttttt
    34741 atggctaaat tctgaatcag cgtaaaaaat gggaagttgc tttttgctgc cgtctgaaag
    34801 acaggtaaaa caaaaaccac atcgccaata agggtctaag tcaactattt cagactaaag
    34861 cgcatctctt tttccccatt tccggcatcc actcaccaca aaggtcgcaa aatctggtaa
    34921 actatcatcc aattttctgc ccaaatgtcg ggtattgctc attttttgtt tgattttcga
    34981 acaaagagag tcgttcctta ttgggtaaca caacttctga ctggccacct ggtggctggt
    35041 agcagtaaaa attctgacta tacctggagg ttttcatggc tgtcgctgcc aacaaacgtt
    35101 cggtaatgac gctgttttcc ggtcctactg acatctatag ccatcaggtc cgcattgtgc
    35161 tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga aaaggacaat ccgcctcagg
    35221 atctgattga cctcaacccg aatcagagcg ttccgaccct ggtggatcgt gagctgaccc
    35281 tgtgggaatc tcgcatcatt atggaatatc tggatgagcg tttcccgcat ccgccactga
    35341 tgcctgttta cccggtagct cgcggtgaaa gccgtctgta catgcatcgc atcgaaaaag
    35401 actggtacac gctgatgaac actatcatca acggttcagc ttctgaagca gatgccgcac
    35461 gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt cttcggtcag aagccgtact
    35521 tcctgagcga tgagttcagc ctggtcgatt gctaccttgc tccgctgctg tggcgtctgc
    35581 cgcaactggg catcgagttc agcggcccgg gtgcgaaaga gctgaaaggc tatatgaccc
    35641 gcgtctttga gcgtgactct ttccttgctt ctttaactga agcagaacgt gaaatgcgtc
    35701 tgggccggag ttaatctgta tggatttgtc acagctaaca ccacgtcgtc cctatctgct
    35761 gcgtgcattc tatgagtggt tgctggataa ccagctcacg ccgcacctgg tggtggatgt
    35821 gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt gacgggcaaa tcgtactcaa
    35881 cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtgc gctttaacgc
    35941 gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc tggctatcta
    36001 cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg atgaagatac
    36061 cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt cggttattga
    36121 tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc cgcagccacc
    36181 acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaacaggcc caggcggcct
    36241 gttttgtctt tttaatgact taataaaata gcgtgattgt ggcaatcata ttcaccacga
    36301 aaccgactgc catggggatc gctgtccgct tcaccaccgc aaaaggcgaa acgcctgcaa
    36361 ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac cgcgcgcgct agcgtcgtgg
    36421 caaaatgcat cggcataatc attacaaccg ctggtacatg tagtccggct gcgatattcg
    36481 gaataagact ggcaaatggc ataaacggcg cattgccaga gcccatcaca atggcacaaa
    36541 tggcaatgac tagcgccata ataatcatca cgccaatacc gcccagacca gaatgctccg
    36601 cacccctgat aaccgcatca acagtgccaa tcgtcgttaa gcctttcgca aatatttcac
    36661 ccgcgaccac cagcgttacc acgttgacaa actgcgtacc catgccgtca aaaaacgcct
    36721 gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa gaactcaaca aacatcgtca
    36781 ccgtcaaact tagtaacatc accaccacca gatgcagttc tgactgcatc agcccgacgt
    36841 gggcgaggaa cagcgagccg agcatcagga ttaacggcat cacaggtaaa atggcgtaat
    36901 agagcggcgg gacattatcg agagtttttt gctctgcctg ttcgtgattg atatttttat
    36961 cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat cgccaccgag ataatgacgc
    37021 aagaggcgac cggaagctgg tagtggaaga agtatgtggc aattttcatt cccgcgacct
    37081 gggctgcaaa aatggagttc gtttccagaa tcccccactc aatggacatc gtggttgcga
    37141 taaccgccac cgcagagagg cgacttactc ccagactcac cagcgtcgga aataaggtga
    37201 ccatcagcaa catacccaga ccggaggcgc tggtaataaa ctgcgccatg atttggccga
    37261 tgacgtaagt tgccgacaga ataatatacg gcgagcgaat gagttttaac gggcggctta
    37321 gcaagctcac catcgcgcaa ctggctccta tgcgctccat gtagcgggca taaccgccca
    37381 ccgccataat cgacagcccc aatccggcaa tgcggttgga cagcatgcgc aggatttcat
    37441 tataaatgtc gacgatcaag tagccgctgc ttttggcggg atcgagtaca ccaccgaacc
    37501 ctaaccagac tccgcacatc atcaggaaga cacctgcggc agccagcacc acctgaggtt
    37561 tgtagttttt caggatcaaa tagcccatcg taattaatac gatgacagat ataattatgc
    37621 cgaacatt
//
LOCUS       DAETQL010000034        36587 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.034, whole genome shotgun sequence.
ACCESSION   DAETQL010000034 DAETQL010000000
VERSION     DAETQL010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.034"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(156..1391)
                     /locus_tag="K0W65_001860"
     CDS             complement(156..1391)
                     /locus_tag="K0W65_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBI3095144.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="K0W65_001861"
     tRNA            complement(1538..1614)
                     /locus_tag="K0W65_001861"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="K0W65_001862"
     tRNA            complement(1657..1743)
                     /locus_tag="K0W65_001862"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="K0W65_001863"
     tRNA            complement(1764..1839)
                     /locus_tag="K0W65_001863"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="K0W65_001864"
     tRNA            complement(1898..1974)
                     /locus_tag="K0W65_001864"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="K0W65_001865"
     CDS             complement(2077..3462)
                     /locus_tag="K0W65_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI3095145.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="K0W65_001866"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="K0W65_001866"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBI3095146.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="K0W65_001867"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="K0W65_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HBI3095147.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="K0W65_001868"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="K0W65_001868"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBI3095148.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="K0W65_001869"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="K0W65_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBI3095149.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="K0W65_001870"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="K0W65_001870"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBI3095150.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="K0W65_001871"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="K0W65_001871"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HBI3095151.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="K0W65_001872"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="K0W65_001872"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBI3095152.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="K0W65_001873"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="K0W65_001873"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBI3095153.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="K0W65_001874"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="K0W65_001874"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBI3095154.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="K0W65_001875"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="K0W65_001875"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBI3095155.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="K0W65_001876"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="K0W65_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBI3095156.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="K0W65_001877"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="K0W65_001877"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBI3095157.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="K0W65_001878"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="K0W65_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBI3095158.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="K0W65_001879"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="K0W65_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HBI3095159.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="K0W65_001880"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="K0W65_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBI3095160.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="K0W65_001881"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="K0W65_001881"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBI3095161.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="K0W65_001882"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="K0W65_001882"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBI3095162.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="K0W65_001883"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="K0W65_001883"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBI3095163.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="K0W65_001884"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="K0W65_001884"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBI3095164.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="K0W65_001885"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="K0W65_001885"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBI3095165.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="K0W65_001886"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="K0W65_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBI3095166.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="K0W65_001887"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="K0W65_001887"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBI3095167.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="K0W65_001888"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="K0W65_001888"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBI3095168.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="K0W65_001889"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="K0W65_001889"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBI3095169.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="K0W65_001890"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="K0W65_001890"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBI3095170.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="K0W65_001891"
     CDS             complement(31267..32913)
                     /locus_tag="K0W65_001891"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBI3095171.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="K0W65_001892"
     CDS             complement(32916..32966)
                     /locus_tag="K0W65_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095172.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="K0W65_001893"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="K0W65_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBI3095173.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..34981
                     /locus_tag="K0W65_001894"
                     /pseudo
     CDS             33502..34981
                     /locus_tag="K0W65_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="K0W65_001895"
     CDS             complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="K0W65_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBI3095174.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35463..36302
                     /gene="hdfR"
                     /locus_tag="K0W65_001896"
     CDS             35463..36302
                     /gene="hdfR"
                     /locus_tag="K0W65_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBI3095175.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36398..36473)
                     /locus_tag="K0W65_001897"
     tRNA            complement(36398..36473)
                     /locus_tag="K0W65_001897"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36438..36440),aa:Trp,seq:cca)
     gene            complement(36482..36558)
                     /locus_tag="K0W65_001898"
     tRNA            complement(36482..36558)
                     /locus_tag="K0W65_001898"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36522..36524),aa:Asp,seq:gtc)
BASE COUNT         8941 a        10012 c         9216 g         8418 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acggacgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcactgg agatcccatt
    34621 accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa
    34681 acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg
    34741 gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg cgatgtggtt
    34801 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tactgaaagt
    34861 tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga
    34921 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
    34981 aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct tcagcacctt
    35041 caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta atacgcgtca
    35101 tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca cggcatactg
    35161 ctacaaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga
    35221 aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca ccatgacgag
    35281 agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg
    35341 ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg
    35401 cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca
    35461 ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc
    35521 gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc agacaactgg
    35581 aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt ttaaccgctg
    35641 ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgca
    35701 aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc gcctcgttgt
    35761 gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat gcccatacag
    35821 gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ctgcatgaac
    35881 gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt agtagtcagt
    35941 tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta aagggagatc
    36001 ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca ggtttgatcg
    36061 gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag caacagattg
    36121 cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc
    36181 ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg ctgcaaaata
    36241 gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat gaagtgtatt
    36301 aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg attatttcag
    36361 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
    36421 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
    36481 gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac
    36541 cgactgaact accgctccac cgaattcttt tacaaccacc ggtttta
//
LOCUS       DAETQL010000035        35759 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.035, whole genome shotgun sequence.
ACCESSION   DAETQL010000035 DAETQL010000000
VERSION     DAETQL010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.035"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..247
                     /locus_tag="K0W65_001899"
     CDS             <1..247
                     /locus_tag="K0W65_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBI3095176.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="K0W65_001900"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="K0W65_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBI3095177.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="K0W65_001901"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="K0W65_001901"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBI3095178.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="K0W65_001902"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="K0W65_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HBI3095179.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /locus_tag="K0W65_001903"
     CDS             complement(3258..3542)
                     /locus_tag="K0W65_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095180.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="K0W65_001904"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="K0W65_001904"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBI3095181.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="K0W65_001905"
     CDS             complement(4443..5651)
                     /locus_tag="K0W65_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3095182.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /gene="rcdA"
                     /locus_tag="K0W65_001906"
     CDS             5735..6271
                     /gene="rcdA"
                     /locus_tag="K0W65_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HBI3095183.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="K0W65_001907"
     CDS             complement(6446..8131)
                     /locus_tag="K0W65_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBI3095184.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="K0W65_001908"
     CDS             8401..8778
                     /locus_tag="K0W65_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HBI3095185.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="K0W65_001909"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="K0W65_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBI3095186.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="K0W65_001910"
     CDS             9225..9512
                     /locus_tag="K0W65_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBI3095187.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="K0W65_001911"
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="K0W65_001911"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HBI3095188.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="K0W65_001912"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="K0W65_001912"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBI3095189.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="K0W65_001913"
     CDS             11269..11745
                     /locus_tag="K0W65_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBI3095190.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="K0W65_001914"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="K0W65_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBI3095191.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14437
                     /gene="potG"
                     /locus_tag="K0W65_001915"
     CDS             13304..14437
                     /gene="potG"
                     /locus_tag="K0W65_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBI3095192.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="K0W65_001916"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="K0W65_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBI3095193.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="K0W65_001917"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="K0W65_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBI3095194.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="K0W65_001918"
     CDS             16302..16790
                     /locus_tag="K0W65_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBI3095195.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="K0W65_001919"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="K0W65_001919"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBI3095196.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="K0W65_001920"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="K0W65_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HBI3095197.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="K0W65_001921"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="K0W65_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBI3095198.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="K0W65_001922"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="K0W65_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBI3095199.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="K0W65_001923"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="K0W65_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3095200.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="K0W65_001924"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="K0W65_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBI3095201.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="K0W65_001925"
     CDS             complement(22242..22757)
                     /locus_tag="K0W65_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI3095202.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDTRNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..25175
                     /locus_tag="K0W65_001926"
     CDS             23406..25175
                     /locus_tag="K0W65_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBI3095203.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="K0W65_001927"
     CDS             25386..25709
                     /locus_tag="K0W65_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBI3095204.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /gene="amiD"
                     /locus_tag="K0W65_001928"
     CDS             25706..26536
                     /gene="amiD"
                     /locus_tag="K0W65_001928"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HBI3095205.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="K0W65_001929"
     CDS             complement(26533..27546)
                     /locus_tag="K0W65_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBI3095206.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..29075)
                     /locus_tag="K0W65_001930"
     CDS             complement(27645..29075)
                     /locus_tag="K0W65_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HBI3095207.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="K0W65_001931"
     CDS             complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="K0W65_001931"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBI3095208.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="K0W65_001932"
     CDS             complement(30124..31842)
                     /gene="poxB"
                     /locus_tag="K0W65_001932"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBI3095209.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="K0W65_001933"
     CDS             complement(31975..32943)
                     /gene="hcr"
                     /locus_tag="K0W65_001933"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBI3095210.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="K0W65_001934"
                     /gene_synonym="priS"
     CDS             complement(32955..34607)
                     /gene="hcp"
                     /locus_tag="K0W65_001934"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBI3095211.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILPLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="K0W65_001935"
     CDS             complement(34751..35650)
                     /gene="lysO"
                     /locus_tag="K0W65_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBI3095212.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8910 a         8839 c         9193 g         8817 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat
     2341 aagcttctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggc gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccat
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattgtag cctgcccggt attttgccgc
     3721 tgcgacgact gcgctgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcattac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaggc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc gcgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatgctaatt tacgataatg tgttttacca gaattcaggg cgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tcagccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggcaaa acgtcttgcc ccacagctga tgaactgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg ctccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta tttactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt
    11341 gaatgtgata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 ctagaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgccagcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccgtgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcttggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gtgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctgg ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagctgtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg atccgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcttggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggg cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctagtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggaaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca tcaagtgaat ttatatgcaa taaacatgat
    18961 taaataattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaactggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 tttccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgcgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg gggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gtatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggcgac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tataaataca aaaggcgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tgggtgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat cccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatactttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaac ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata atcgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcct gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga ggcgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gcgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat tgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28381 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28441 acctctggtc cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc
    28501 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28561 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28621 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28681 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28741 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28801 agagccagct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28861 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
    28921 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28981 gccgctagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    29041 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    29101 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    29161 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29221 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29281 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29341 caaccgtgcg acgttatttt ccagggcata catcccggcg gcagccagaa tcccggactg
    29401 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29461 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29521 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29581 gcgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29641 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29701 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29761 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29821 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29881 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29941 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    30001 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    30061 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    30121 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30181 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30241 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30301 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30361 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30421 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30481 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    30541 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30601 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30661 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30721 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30781 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagcgc
    30841 tttatccaga aacttgcgat cggttttttc ttccaccagt ggcagcaatg cgcgcagggt
    30901 tgatttgata tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg
    30961 attaatatca atctgaataa ttttggcatc ggtcgggtag aacgcacggt aggggaactg
    31021 agtaccgagt agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaatcc
    31081 gattaacccg gtcattccaa catcatacgg attatcgtat tcgacatgct ctttaccgcg
    31141 tagggcatga acaataggtg ctttaatttt cccggcaaac tcaactaact ctttatgcgc
    31201 ccccgcgcag ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc
    31261 cagtttgcgt aactcttctt cttccggcat cacgactggc tgtggcgcat gataccagtg
    31321 cgtggttgcc ccttctggcg caggttttaa cgccacgtcg cccggtaata caacaacgga
    31381 aacgccacgg ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc
    31441 cgggctggaa accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt
    31501 ttcctggaaa tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac
    31561 cggaacgtta ttgcggtggc aatcgaatag gccgttgatc aagtgcaggt tgcctgggcc
    31621 gcacgatccg gcacagaccg ccagttctcc tgaaagttgt gcttcagcgc cagcggcaaa
    31681 cgccgccact tcttcatggc gggtggacat ccactcgatg gtgcccatgc gattaagact
    31741 gtcgctaaga ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga
    31801 ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa
    31861 tgtgataacg gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg
    31921 aggcactaac ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc
    31981 gagaaccaaa tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc
    32041 ggcgtcggtc agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt
    32101 acagcagccg caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag
    32161 cgcctccagt agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt
    32221 gaatttcaga ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa
    32281 acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc
    32341 gcaggtcatc acggtacgtg aagctaagtc aggtacatct gccagcagtt cgcgagtgag
    32401 gcgaccagcg ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt
    32461 acgccactca tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac
    32521 atcggcctgt ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc
    32581 gcagcctgcc gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc
    32641 catcgcgtcc gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga
    32701 gccgacaccg tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt
    32761 ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata
    32821 ttgcccggcg cgatatgggt agtgatcgtg gcaaatcagg gaaatcgtcc acacatccgg
    32881 cgtttcttgc gtaatgtgat gaacctgtat ccgccacggg cattgattcg ttggcatcgt
    32941 catcgacaaa ctccttacgc gttcaacagt tgcttcatgt cttcttcaac agtggtgata
    33001 gaacgcaggc cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt
    33061 gcagtcgggc cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgaca
    33121 atcgctttct gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag
    33181 cccagtttct ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga
    33241 cctgcatcta ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga
    33301 tatttaccac aggcgagggg caggatcagg cagtcatccg gcacgctggt ggcgaaatcg
    33361 gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc
    33421 agtttttcac ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga
    33481 ccaaagccca cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc
    33541 gcctgggcga tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca
    33601 acgatgctgc gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag
    33661 ttcgaggtca tcacgatggg gccagggaaa cgagcgaact ccacttgctg attctgccag
    33721 ccgctgccgt agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca
    33781 ggcagcattt cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg
    33841 ttgtagagat ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg
    33901 acgttgacct gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg
    33961 ctcatcactt tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata
    34021 tcggcaggcc aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg
    34081 tcgtattgac cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac
    34141 aggcacagca gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg
    34201 gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat cgctcaccag ttgcaggtca
    34261 gccatcgggt tatcgacgcg cgcgttggca tctacagcca ggcattgtgc tttcagcgcc
    34321 tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg
    34381 ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg
    34441 ccgtattcac gcgcttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa
    34501 tcctgaaggt cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg
    34561 ttttctgccg gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt
    34621 aaagttatat ttaatataca tgtttaaggg taagacgctt aacgcgggga taaaagggat
    34681 ttttcatgca actttaaggg agattgattt agcgcaattt tggcggcagg gaactaccgc
    34741 cagagaggta ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa
    34801 aaccgtgaac aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca
    34861 gggtgaaatc cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa
    34921 tcagcccagg gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg
    34981 cgctcccgat taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc
    35041 cgaaaccgga ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa
    35101 ttaaaccacc aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat
    35161 taaggacaat ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa
    35221 gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac
    35281 tcagaccaat ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca
    35341 tcgcaatacg cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct
    35401 ccagccacat cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat
    35461 aatgcagaat cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca
    35521 taaaaaagag aataaggtaa accatccagc ttaatagctg attaatagct tttaacgcag
    35581 cttgttggcg aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc
    35641 cagaaaacat gaaaatccag tccttgcaaa gaagaagtcg aaatgcgcga tgacacacta
    35701 ctgaaagcgg aagggcgagt aaagttgcaa ttaaaaggaa atgttatgca tcagagggg
//
LOCUS       DAETQL010000036        35224 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.036, whole genome shotgun sequence.
ACCESSION   DAETQL010000036 DAETQL010000000
VERSION     DAETQL010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.036"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            86..862
                     /gene="exuR"
                     /locus_tag="K0W65_001936"
     CDS             86..862
                     /gene="exuR"
                     /locus_tag="K0W65_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBI3095213.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1207..1869
                     /locus_tag="K0W65_001937"
     CDS             1207..1869
                     /locus_tag="K0W65_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBI3095214.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1873..2256
                     /gene="mzrA"
                     /locus_tag="K0W65_001938"
     CDS             1873..2256
                     /gene="mzrA"
                     /locus_tag="K0W65_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBI3095215.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2403..2757
                     /locus_tag="K0W65_001939"
                     /pseudo
     CDS             2403..2757
                     /locus_tag="K0W65_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2795..3100
                     /locus_tag="K0W65_001940"
     CDS             2795..3100
                     /locus_tag="K0W65_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBI3095216.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3103..3507
                     /locus_tag="K0W65_001941"
     CDS             3103..3507
                     /locus_tag="K0W65_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBI3095217.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3497..3796
                     /locus_tag="K0W65_001942"
     CDS             3497..3796
                     /locus_tag="K0W65_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HBI3095218.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3982..4278
                     /locus_tag="K0W65_001943"
     CDS             3982..4278
                     /locus_tag="K0W65_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBI3095219.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4348..5334
                     /gene="yqjG"
                     /locus_tag="K0W65_001944"
     CDS             4348..5334
                     /gene="yqjG"
                     /locus_tag="K0W65_001944"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HBI3095220.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5627..5995
                     /locus_tag="K0W65_001945"
     CDS             5627..5995
                     /locus_tag="K0W65_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBI3095221.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="K0W65_001946"
     CDS             complement(6202..7098)
                     /gene="yhaJ"
                     /locus_tag="K0W65_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HBI3095222.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7203..7904
                     /locus_tag="K0W65_001947"
     CDS             7203..7904
                     /locus_tag="K0W65_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBI3095223.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7927..8091
                     /locus_tag="K0W65_001948"
     CDS             7927..8091
                     /locus_tag="K0W65_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095224.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8303..9613)
                     /locus_tag="K0W65_001949"
     CDS             complement(8303..9613)
                     /locus_tag="K0W65_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBI3095225.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9641..10972)
                     /locus_tag="K0W65_001950"
     CDS             complement(9641..10972)
                     /locus_tag="K0W65_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBI3095226.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="K0W65_001951"
     CDS             complement(11247..12611)
                     /gene="tdcG"
                     /locus_tag="K0W65_001951"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBI3095227.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12683..13072)
                     /locus_tag="K0W65_001952"
     CDS             complement(12683..13072)
                     /locus_tag="K0W65_001952"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBI3095228.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="K0W65_001953"
     CDS             complement(13086..15380)
                     /gene="tdcE"
                     /locus_tag="K0W65_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HBI3095229.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="K0W65_001954"
     CDS             complement(15414..16622)
                     /gene="tdcD"
                     /locus_tag="K0W65_001954"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBI3095230.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="K0W65_001955"
     CDS             complement(16648..17979)
                     /gene="tdcC"
                     /locus_tag="K0W65_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBI3095231.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="K0W65_001956"
     CDS             complement(18001..18990)
                     /gene="tdcB"
                     /locus_tag="K0W65_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBI3095232.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="K0W65_001957"
     CDS             complement(19089..20027)
                     /gene="tdcA"
                     /locus_tag="K0W65_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBI3095233.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20217..20561
                     /gene="tdcR"
                     /locus_tag="K0W65_001958"
     CDS             20217..20561
                     /gene="tdcR"
                     /locus_tag="K0W65_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HBI3095234.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20817..21357
                     /locus_tag="K0W65_001959"
                     /pseudo
     CDS             20817..21357
                     /locus_tag="K0W65_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21379..21726
                     /locus_tag="K0W65_001960"
     CDS             21379..21726
                     /locus_tag="K0W65_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095235.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21895..22545
                     /locus_tag="K0W65_001961"
     CDS             21895..22545
                     /locus_tag="K0W65_001961"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095236.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(23166..23542)
                     /gene="rnpB"
                     /locus_tag="K0W65_001962"
     ncRNA           complement(23166..23542)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="K0W65_001962"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(23575..24720)
                     /gene="garK"
                     /locus_tag="K0W65_001963"
     CDS             complement(23575..24720)
                     /gene="garK"
                     /locus_tag="K0W65_001963"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HBI3095237.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(24817..25707)
                     /gene="garR"
                     /locus_tag="K0W65_001964"
     CDS             complement(24817..25707)
                     /gene="garR"
                     /locus_tag="K0W65_001964"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBI3095238.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(25737..26507)
                     /gene="garL"
                     /locus_tag="K0W65_001965"
                     /pseudo
     CDS             complement(25737..26507)
                     /gene="garL"
                     /locus_tag="K0W65_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(26523..27857)
                     /gene="garP"
                     /locus_tag="K0W65_001966"
     CDS             complement(26523..27857)
                     /gene="garP"
                     /locus_tag="K0W65_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HBI3095239.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            28232..29803
                     /gene="garD"
                     /locus_tag="K0W65_001967"
     CDS             28232..29803
                     /gene="garD"
                     /locus_tag="K0W65_001967"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBI3095240.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(29855..30679)
                     /gene="agaR"
                     /locus_tag="K0W65_001968"
     CDS             complement(29855..30679)
                     /gene="agaR"
                     /locus_tag="K0W65_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HBI3095241.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            30928..32208
                     /gene="kbaZ"
                     /locus_tag="K0W65_001969"
     CDS             30928..32208
                     /gene="kbaZ"
                     /locus_tag="K0W65_001969"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HBI3095242.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            32231..32704
                     /gene="agaV"
                     /locus_tag="K0W65_001970"
     CDS             32231..32704
                     /gene="agaV"
                     /locus_tag="K0W65_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HBI3095243.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            32715..33494
                     /locus_tag="K0W65_001971"
     CDS             32715..33494
                     /locus_tag="K0W65_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HBI3095244.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            33484..34293
                     /locus_tag="K0W65_001972"
     CDS             33484..34293
                     /locus_tag="K0W65_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HBI3095245.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            34380..34814
                     /locus_tag="K0W65_001973"
     CDS             34380..34814
                     /locus_tag="K0W65_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBI3095246.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            34811..>35224
                     /locus_tag="K0W65_001974"
     CDS             34811..>35224
                     /locus_tag="K0W65_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBI3095247.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         9122 a         9092 c         8413 g         8597 t
ORIGIN      
        1 tcatcaaatt tatatatgac caatggcata cccatgaata aagtggtatg ataacttcgt
       61 ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt tgtatcaaca
      121 acttgctgct gacctgaaag agcgcattga acagggcgtc tatctggtgg gtgataaact
      181 gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag ttcgtgaagc
      241 catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag gtattcatgt
      301 ggtttccaac cagccgcgtc atcagcaggc ggctgacaat aatatggaat tcgccaatta
      361 cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg ccgagttcgc
      421 ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg aacaggcgcg
      481 cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc aggtagccct
      541 ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc agcgtagcca
      601 taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg ataactggtg
      661 tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatcctcatg ctgctaagct
      721 ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa ccagcgacga
      781 tttcgagttc aatgccgacc gctatctgtt cgccgaaaac ccggtagtgc atctcgatac
      841 ggccaccagc ggcagcaaat gaaattcctg tccgacaggc gctgcaatgc gcctgtttgt
      901 ctcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg ccaccctccc
      961 ctgcaatcag caaaatcaga ctccacggac atgtaatttt gataacgaac aacgttgacc
     1021 tttgttacaa ttagattcaa tttgaattta tgcttttgaa tgctttctta tctcacgttt
     1081 taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata ccaaaacagc
     1141 aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat tcaccaggaa
     1201 taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga ttttgaaacc
     1261 ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat tttgttcctt
     1321 gaaaatggct tgcttccggc ggccttttta ccgggcgaca gtttactggt attggtcggc
     1381 gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct gaccgttgcc
     1441 gccagcctcg gttgctgggt cagctatatt caggggcgat ggctgggcaa tacccgcacc
     1501 gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca ccatcttttt
     1561 cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcatttgt cagaacactg
     1621 ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt cttcaactgg
     1681 atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct cggcaaaacg
     1741 ccggtatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct cccggtggtg
     1801 ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa atatggaaat
     1861 cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg gtccggcact
     1921 gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca gcaagagtct
     1981 acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt ttcgatctgg
     2041 catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa cgacactctg
     2101 ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct cgacagaaca
     2161 ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat gcagtggctg
     2221 acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg aaacatatca
     2281 ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt taacactacc
     2341 gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg gaaggttcaa
     2401 gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc ggtagttatg
     2461 ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc tatgccgaaa
     2521 aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa gtccgggcca
     2581 actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag cagaaagatg
     2641 aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat gccgatagaa
     2701 cggaaactgg cagaagcgca ggaagagctg aaaaagctgg aagcgcgcga ctactaactc
     2761 acaatagtca ctacttactc acctggagaa aactatgtcg aaagaacaca ctacggaaca
     2821 tctgcgtgct gagttgaaat ccctttccga tacgctggaa gaggtgctta gctcatctgg
     2881 cgagaagtcg aaagaagagt tgagtaagat tcgtagcaaa gcggagcagg cactgaaaca
     2941 gagccgttat cgcctgggtg aaaccggtga tgccatcgcc aaacaaaccc gtgtcgcggc
     3001 ggcgcgtgct gatgagtatg tgcgtgaaaa tccgtggacc ggcgtgggaa ttggcgctgc
     3061 aatcggtgta gtgctcggcg ttctgctgtc gcgtcgttaa ttatggcgga cactcatcac
     3121 gcgcaagggc ccggtaaaag cgttctgggc atcgggcagc gaattgtttc catcatggtt
     3181 gaaatggtgg aaacacgtct gcggctggcg gtggtggagc tggaagagga aaaagctaat
     3241 ctctttcaac ttttactgat gctgggcctg acgatgcttt tcgctgcatt tggtcttatg
     3301 agcctgatgg tgctaattat ttgggcggtt gatccgcaat atcgcctgaa tgcgatgatt
     3361 gccaccaccg tggtgttgct gctactggca ctgattggcg gtatctggac gttacgtaaa
     3421 tcgcgtaaat ccaccttgct gcgccataca cgccatgagc tggcaaacga tcggcaactg
     3481 ctcgaggagg agtcccgtga gcagtaaagt cgaacgtgaa cgacgtaagg cgcaactgct
     3541 tagccagatc cagcaacaac ggctggatat ttccgccagt agtcgtgaat ggctggaggc
     3601 aacaggcgct tacgatcgtc gctggaatat gctgctaagt ctgcgctcct gggcgctggt
     3661 tggcagtaga gtgatggcga tctggacgat tcgccatcct aatatgctgg tccgctgggc
     3721 cagacgcggt tttggcgtat ggagcgcctg gcgtctggtt aaaacgaccc tcaagcagca
     3781 acagcttcgc ggttaacttc cctctggccg gagcaattcc ggccttatcc atcaaatttt
     3841 ttgaatattt ttgacagttt tccttgctaa caatcatcat tcaccacgtt tatgattctc
     3901 tccatcgaca gcaacgacgc taatagcgcg cccttgcaca aaaaaacaat cagcagcctg
     3961 agtggcgcag tggagagtat gatgaaaaaa ttagaagatg ttggtgtact ggtagcgcac
     4021 attttaatgc cgattctgtt tattaccgca ggctggggaa aaatcaccgc ttactctggt
     4081 acccaacaat atatggaagc aatgggtgtc ccgggtttta tgctgctgac ggcattttta
     4141 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4201 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cggccgcctg
     4261 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4321 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4381 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4441 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4501 aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4561 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4621 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4681 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4741 actgttcccg tgctgtggga caaaaagaac cacaccatcg tcagcaacga atcagcagaa
     4801 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg tgattactac
     4861 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     4921 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     4981 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5041 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5101 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5161 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5221 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5281 ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg ttaaaaaaag
     5341 gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt cggatgcggc
     5401 gtgaacgcct tatcaaacct acaaagcgtt tatattcaat atgttacaat gaccgcgtag
     5461 gccagataag cgtagcgcat ctggcgattt tgcgcttgcc atcagtctta gcgcgccttc
     5521 acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac gtctattctt
     5581 aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg actggtatct
     5641 gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag agtactggat
     5701 gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata aaatgttagg
     5761 ctggcaacgc gctggtggcg aaggcatcct gacgataatc tacggtattc tggtgttttt
     5821 accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg cgtggtgggc
     5881 actgctattc ttaatcccgt ttatcggctg gcttattatc atcgtcttta actgccaggc
     5941 gggtacgcca ggcgaaaacc gctttggacc agatccaaaa ctggaacaag aataatgatt
     6001 aagcaccttg taaaaggtgc ttcaaattgt tgacaaagtg cgctttgttt atgccggatg
     6061 cggcgtaaac gccttatcca gcctacaaat tgccaaaatt caatatattg cagtgactgc
     6121 gtaggcctga taagcgtagc gcatcaggca gttttgcgtt tgtcagcagt cagaagcacc
     6181 ttgtgaaagg tgcttaatag attattttcc agcgaacaac tttggaattt cccgcagaca
     6241 ccaggatttc gcttccccca tactgtcacg acgccaggcc ataataatat cgatctcgct
     6301 ggtcgattcc gggctgacga cacgcaaccg cccttccgca atatcttttt cgaccatcgg
     6361 atacggcatc gtcgccacgc caagccccgc cagtaatgcc tgacgtttat cttcaatcgt
     6421 gctcaccgtt aagcgcggct gtttgtccag cagctgtacg gtcaacaccg ggcgctcacg
     6481 agcggtatcc gctaccgcaa ttccacgata tttcacacgc gtcacttcag ataacggttc
     6541 cggctcctga tgaatcgggt gatcaggcgc ggcaacgtag acgttcatta acgtatagag
     6601 cttgcgcgag ttgatctccg acgaggaacg aaaatgcata tccggcgcga taacgatatc
     6661 cgcccgcccc tgctccagcc gttcccacgc ccccgccagc acttctgtga tgattgccag
     6721 ttgggtattg gcttttgccg ccagtttgtc gattaacggg aaaaaggcag gtgtcggtac
     6781 cagcgcttcg gtcacaatgg tgagatgcgt ttcccaaccg cgcgcgagag cttccgcatc
     6841 agtagtcagt ttatctgcgg cttccagcaa aacgcgccct cgctccagca acatcctccc
     6901 gacattggtg aatttggtgc gatggcccga gcggtcaaac agcaccacat ccagctcttc
     6961 ttccagtttt tgcatggtgt agctaagtgc ggaaggcacg cgtcccagct catccgctgc
     7021 cgccgcaaaa ctgccccggc gatcgatcgc atccataacc cgtagtgctt ccagcgttaa
     7081 tgccctttct ttggccattt cgttctcatt caggaaattt gaacataccg ggcagaatat
     7141 ctggctaaca atgtagcgtc cagcccctta ccataaaagg aagtaaagag aggtcaagaa
     7201 ttatgattac tacccgaact gccaggcagt gtggacaagc agactacgga tggttgcagg
     7261 cccggtatac tttttccttt ggacactact tcgacccgaa attgttaggc tatgcctccc
     7321 tgcgtgtgct taaccaggaa gtgctggccc caggtgccgc ctttcagccg cgaacctatc
     7381 ccaaagtcga tattttaaat gtgattctgg atggagaagc agagtatcgc gacagcgaag
     7441 gcaatcatgt tcaggccagc gccggagagg cgttgctgct ctctacccag ccgggtgtca
     7501 gctatagcga acacaatctc agcaaagaca aaccgttaac gcgaatgcag ctttggctgg
     7561 atacctgccc gcagcgagag aatccgctga ttcaaaagct ggcgcttaat atggacaagc
     7621 agcaattaat cgcctcgcca gagggtgcga tgggaagcct gcaattacgc caacaagtgt
     7681 ggctgcacca tatcgtgctc gacaaaggcg aaagtgcgaa tttccagttg catgggccac
     7741 gcgcgtattt gcaatcgatt cacgggaaat ttcatgcgct tacgcatcat gaagagaaag
     7801 cggcgctaac ctgcggtgat ggggcgttta ttcgtgacga ggctaacatt acgctggttg
     7861 ccgattcccc actgcgcgct ttgctgatag atttgcctgt ctagttgttt ttacagggag
     7921 atgatgatga gtaaaaaatc gaccaaaaag cgccagccag cgaagcccgt ggtggcgaaa
     7981 gaacctgctc gcaccgccaa aaattttggc tatgaagaga tgttgagcga gctggaagct
     8041 atcgtcgcgg atgctgaaac gcgtttagcc gaggatgaag ctaccgcgta aagacaggtt
     8101 gccggatgcg gcgtaaacgc cttatccggc ctacaaaaca caacaacatc aacaaattaa
     8161 acaccccgta ggccggataa gatgcgccag catcgcatcc ggcatcaggt acaaaacaca
     8221 acaacatcaa caagttgagc accccgtagg ccggataagg tgcgccagca tcgcatccgg
     8281 cacaatactc aaaacctggc gtttatctgg ccttgctcgc cataatctcg ataatctgcc
     8341 gatccgtttg ctgcatcgaa tggcttgcta acgcacacag gttggcaatc gactgctcaa
     8401 catcatgcgc cacgatccct tcattgccgg tcacggcggt atcatccagc gccattaaca
     8461 ccgctttcca cgcagccgaa gcactggtcg aaaccttcat cgcgcagctg ttcgacgcac
     8521 catcgcaaat catgccgctg acatcgccga tcatactgct gatcgccatc gagatagttt
     8581 cataacgccc atccaccagc catgccatcc cggcggcggc ccccattgct gcggtcgttg
     8641 cggcacaaag tgcagacaaa cgcggtaact ggttatggat gtaaattgcg ctcaaatgag
     8701 aaagcatcag cgcacgcgcc agtcgttcat catccgctcc gaagtgttct gctacaacca
     8761 ccacaggcat tattgcagtg atcccctggt tacccgagcc ggagttactc atagccggaa
     8821 gcgtagcgcc gcccatacgc gcatcggatg ccgcgctggt acgaatcaca atggatgaag
     8881 agagatcttt cgccagcaaa ccgcgctcgc actgtttttc cagcgtcgcg ccaatatgca
     8941 gcccccactt accgctcaaa ccttcctgcg ataacgcaca atttagcttc gcggaatcga
     9001 gaataaagcg gatcgccgca aatgggactt cattgacgaa cttcaggatc tcagccagcg
     9061 tcgttctgga aagcaccgtc agcggcgact cttgctcgcc ctctgccaca cacgcctggt
     9121 gggtaaacac cacaccattg tgcgtctcaa tatgcacaat gttggtatgc ccgccgacga
     9181 tggtgacaca cgcccacttc tcaccgttcc agactttggc gcgtgagaag aggatttcat
     9241 tgcaaggttc ctggatctta acagagactt tccccgccgc cagcagtgct ttggcatcgg
     9301 taattgcctg cgcagttgcg tctttcagca cttccagccc ggcgttggca tttccaccta
     9361 acgcccccag cgccgccgca atcggcagcc ccaccattcc cgtgccggga acggtgacgc
     9421 ccagaccgtt cttcatcaga tttggcgaaa cccaggcttc tacacgttca accggacctt
     9481 ccagttctgc cgcagcaacc gccgccgcca gcgccagtga aatcggttca gtacatccca
     9541 gcgccggttt tacttcctcc tgaacggcga ggatgtaacg ctgccataac ggatttaaag
     9601 tcgaatcaaa catattaaaa accttaaaat ttcaggtaaa tcaagaaaat gccaggaaca
     9661 gagaaacaca aagcaacaaa ccagtgacga taatcaggta cagagacatc cctttgtatt
     9721 tgtgcaatgc cggtactttg taaaccagcc acgccgggat caggcaccct accatgccga
     9781 aaatcgggct acagatagag gtgaagctca acaccggtgc gttcagtacg atggcgctcc
     9841 aggccagcaa aatggcgaaa atcatgatgc cgcgctgaac aagattttcg ttaatcttct
     9901 cggcaggcat cttgcgacgc aggatgttca ttacgatccc ttgcgttgct tcgcgaaagc
     9961 ctaaatagac gccaaagaat gcagtcatga cagcaaagat attgagaatg acgctgacca
    10021 ctttcaccca cgctgcgccg tcaccgctaa taaactgtgc ggcaatcgcc agcgcggaaa
    10081 tattctgctc gtaggctttt accgcttcgt catgtcccat cgccagcgtg aacgacacgg
    10141 cgtagaaaaa gacggtgaca aacaaaatgc caaacgcgat attcattgcc cgcaatgctt
    10201 tatgccgcgc cacttcaatt gatttttccc gcgagcgata agagatcacc atcgggctta
    10261 acgtctggat aaacagaatc gacgtcaggg taaacggcag cgtaataatg gcgtttttca
    10321 ccagcagccc cagcggcggt agcgaaccga cgttatacag atgccacatt cccaccatcg
    10381 acacgcccag cgcagcgact accagcagct tggtcagcac catgtcggtc gaaattttga
    10441 ataacaattt ctcgccgcgt gaagagatcg ccaccagaat gcaaatcagt accagaccat
    10501 aaaagggact gtctgacagc aacccttccg tcacgccgaa ggtatgcaga taggaagcac
    10561 tatcgttggt gatggcggtg gaatagacga acatccaaat caccagcatt acgaaataga
    10621 gcgcacctaa caggatgccc cagtttttac ctaaataacc gctaatgaca ctcgggtaat
    10681 ctttacattc tggtgattct gccagcgtat taataaacaa ccgctgaaac agatacattg
    10741 ccgggtaacc aatcaccgat gagagcaaaa atacccacaa tcccatcaaa ccgacctgca
    10801 ccgggagaaa aacaatcccc gcgccaatcg ccatcccgat actcataatc acccagccgg
    10861 tgtcagtact gtcgaatttg atcgcttctc gccactcgct ctcactcatt ccagcacgac
    10921 ccgccggggt cgaagcgtct gcaatgacgc ctttattcga tgcaatttcc ataatttctc
    10981 gctcaatatt ttgtagggct tattattttt ttccgagccg catcaaggcg atatgcggtc
    11041 tacgtttttg caggcgagtt attagaataa agaaatgata cgcgcgaggt cagagaaaat
    11101 cttcacaatc aagactttaa aataacggag atggataaga attttctact aaattaatca
    11161 caagagaata ttcattctct atttgcggca gatcacaaaa aaaaggtgca catttgtgca
    11221 cccaaggatg aaagctgaca gcaatgtcag ccgcagacca ctttaatggc cagtcctccg
    11281 cgtgatgttt cgcggtattt atcgttcata tctttgccgg tttcatacat cgtctcgatc
    11341 actttatcga gtgaaacacg cggtgccgag gtgcggcgca tcgccatccg cgcggcgttt
    11401 actgctttca cggcattaat ggcattacgt tcaatgcacg ggatttgtac ctgtccggca
    11461 accggatcgc aggtcagccc aaggttatgc tccatcgcga tttccgccgc attgcatacc
    11521 tgcgccggac tgccgcccag tagttcagtt aaccctgccg ctgccattga acaggccacg
    11581 ccaatctccc cctgacagcc gacctccgcg ccagagatgg aggcgttcat tttatacagc
    11641 gcgccaatag cccccgctgc cagaaaatag cgggcaattg accgctcgtt taccggacga
    11701 cggaacttat cgtaataagc cagcactgcc ggaataatgc cgcacgcacc gttagtcggt
    11761 gccgttacca cgcgcccgcc agctgcgttt tcttcactaa ccgccagcgc gtacatgttg
    11821 atccagtcga tgacattcat cggatcgtta gagatgttat cgctagaaac cagctgacga
    11881 cgcagcgcta cggcacgaca cggcacattg agcggaccag gcagcacgcc ttcagtgttc
    11941 atcccgcgtt caataccgtc atgcatcact tgccagatac gggcaaaccc tgcgtcaatt
    12001 tccgctttgc tgcgcagcgc cagctcgttg tgcatcatca ggccagatat agacaggccg
    12061 ttgtaatcac acattttcag cagttcacct gctgagtgga aatcgtaagg tacggacgtt
    12121 tcgacatcgt gcgacaggcc gaagtgttct tcttcgacaa taaacccgcc gccgacagag
    12181 taataggttt tacttaatag cgcttcctgt cccttccagg cagtgatccg cattccgttc
    12241 tcatggcgag gcaacatttc gggatggaag ataatgttct ttgctacagg aaaatcaaca
    12301 atatgcgcac cagatgccac tggcagccgc ccgctgcgcg ttactaactc tataaatgca
    12361 gggatctcat caatgacaac atcctgcgga ctgtttcctg ccagtcccat gataatggcg
    12421 acatccgtgg catggccttt gcccgtcagt gacaacgacc cgtacagatc gaccacaata
    12481 tggctcgtcg cggttaataa gccgctactt tccagccgat caataaaact ttttccggca
    12541 ttcattggcc ccacggtatg cgaactggag ggaccaatcc caattttgaa aatatcgaat
    12601 gcactaatca tatccacacc ctcggattgc cgttcagtga agtggagcgg aacgaccttg
    12661 cgaccgtccc gctcacgagg ctttacgcac tacgtactgc gatagcttca atttccagct
    12721 tcacatcttt cggcaaacgc gcaacctgca cacagctccg ggtcgggtag gtcgcctgat
    12781 gctcatcgaa gaactgctta tagacttcat tgatggtggc aaaatcattc agatcggtga
    12841 tgaacacggt catcttgatg atatcgccca cgctcagccc ggcagcaacc acgatcgctt
    12901 tgacgttttc gaggcttaaa cgcgcctgat cttgcacatc agccgggatc tcaccggtct
    12961 gcgggcaaac cggaatttgc ccggaggtga agaccatgct gcctaaatca acgccctgaa
    13021 cataagggcc gattgcgcct ggggcacgtt gcgtttcgat aatctttttc atacatcctc
    13081 cggcgtcaga gcgcctgggt aaaagtacgt gaaataacat cctgttgctg ttcacgggtc
    13141 agtgcgttga agcgcacggc gtagccagag acacggattg tcaggttagg atatttttcc
    13201 gggtgctcga tggcatccag cagcatttcc cgattcatta cgttgacgtt gaggtgttga
    13261 ccgccttcga catccgcttc gtggtggaaa tacccatcca gcaggccgac aaggttggtt
    13321 ttacgtactg gatcttcttt gcccagcgcc gcaggaacga ttgagaaggt gtacgagatc
    13381 ccatctttgg cgtaggtgaa cggcagtttc gccaccgacg tcaatgaggc cacggcacct
    13441 ttgcggtcac gaccatgcat cgggttagcg cccggcgcga acggtgttcc ggcgcgacga
    13501 ccgtccggcg tattaccggt tttctggccg tacaccacgt tagaagtgat agtcagaatc
    13561 gactgggtag ggacggcgtt gcgataggtt ggcagcgctt taattttctt cataaagcgt
    13621 tcaaccaagt cgcaggcaat gctgtctacg cgctcgtcgt tgttgccgta ctgcggatat
    13681 tcaccgtcga tttcaaagtc caccgccagg ccgttttcgt cacggattgg tttcacgcgg
    13741 gcatatttga tggcagacag ggagtccgtc gccaccgaca ggcccgcgat gccgcatgcc
    13801 atagtgcgat agacatcacg atcgtgcagc gccatcagcg aagcttcgta gctgtacttg
    13861 tcgtgcatgt agtgaatgat attcagcgcg ctgatgtact gcaccgccag ccagtccatg
    13921 aagtgatcga ggctgtccat cactttgtcg taatccagca cgtcgtccat cagcggtgct
    13981 gttttcggcc cgacctgaat cttcagcttc tcgtccaccc cgccgttaat tgcgtagagc
    14041 agcgttttcg ccaggttagc gcgtgcacca aagaactgca tttgcttacc aatcaccatt
    14101 gggctgacgc agcaggcaat cgcgtaatcg tcgctgttga agtcagtacg catcagatcg
    14161 tcattttcat actgcaagga agaggtgacg atcgacacct gcgcggcata ttttttgaag
    14221 gcaatcggta attcttccga ccaaagaatg gtcaggttag gttccggtgc cggccccata
    14281 gtgtgcaggg tgtgcaaata gcggaaggag tttttggtca ccagcgtacg accgtccagc
    14341 cccatcccgc cgatcacttc cgtcgcccag attgggtcgc cggagaacag cgaatcaaat
    14401 tccggtgtac gcaggaagcg taccatacgg atcttcatga tgaagtgatc gatcagttcc
    14461 tgtgcctgct gctcattgag gacgccagct ttaaagtcgc gctcaatgta gatatccagg
    14521 aacgatgccg tgcgccccag cgacatcgcg ccgccatttt gcgatttcac tgccgccaga
    14581 taagcgaagt agagccactg caccgcttcc tgtgcattct gcgccgggcg agagatatca
    14641 aagccatatt tcgccgccat ttcctgaatc tgcaacagcg catgacgatg ctctgccagc
    14701 tcctcacgca gacggatggt ggcttccaga tcctcgcctt tttccagacg agactggaga
    14761 tcggcaaatt gcagttcgcg ttcacgcacc agataactga tgccatacag cgctacgcgg
    14821 cgatagtcac cgataatgcg cccacgacca tagccatctg gtaaaccggt cagcacgcca
    14881 gatttacggc agcgcagcat atccggtgag taaacatcaa atacgccctg gttatgggtt
    14941 ttacgcagat cggtaaacag atattcaaat tcactgtcca tttcacggcc ataggcgtgg
    15001 aatgaacttt taatcatatt aatgccaccg aacgggtgta gcgcacgttt caacggcgca
    15061 tccgtttgca ggccaacaat tttttccagc ggctggttaa tatatcccgc atcatgagcg
    15121 gtaattgtgg tggcaatatt ggtatcgaaa tcaaccggcg cgtgggttgc gttttcgata
    15181 cggatgcctt ccattacctt ttcccacaat tccgtggtgg caggcgtcgc ttcggcgaga
    15241 aaagattcat cgccttcata cggtgtatag ttatgttgaa taaaatcgcg gacattaatt
    15301 tcgcttttcc agtccgtacc tttaaagcca agccatgcgt cagcgtacag cttatcgctg
    15361 gtatcaatat ctaccttcat gaaaaataat ctctctacaa tacttcaact aaattatgca
    15421 aattctgcgg gcgcgttaac tttacctaaa tgaatggcat ccaaagcaat cattttttct
    15481 tcgttagtcg gaataacgac gcaaatgaca tgcgcatttt cactggaaac aattcgctcg
    15541 ccaaaagagt tagaacgatt attcatttct gtatcaatct ctacgcctaa tacagccaaa
    15601 tgttccatga ccagacgacg aattaaactt gaattctctc ctattccgcc ggtgaatata
    15661 attccatcca gccgacgtaa tgaagctgca tgtccggcaa tatgacgggc aattcggtga
    15721 acaaaggttt taattgccag ttgcgcgcgt tcgtgacctt catgccaggc tttttccaga
    15781 acacgtaaat ccgaagaaag accggaaata cctaataatc ccgactcttt attcactacg
    15841 cgttccaggt cacccaggct ctggttggtt tggctggcga cccaggacat cgcaccaaag
    15901 tcgacatcgc cactgcgggt acccatcatc aagccttcca gcggcgtcat tcccattgag
    15961 gtgtcaacac tctgaccgtt gcgaactgcg cagattgacg cgccattgcc aagatgcgcc
    16021 acaaccaggc cggagtcatc ttccgccaga ttcagcagcg aatgtgcgcg ctgggaaaca
    16081 tagcggtgcg acgtgccgtg gaaaccataa cggcgtacac ctaactcttc ataatatttc
    16141 cacggcaggc cgtataaata agcttccgga gccatcgtct ggtggaaact ggtatcaaat
    16201 accgccacct gagttacgcc cggaaataat tgctgcgccg attcaatacc acttaaattt
    16261 gcgtaattat gcaggggtgc cagtggagaa acgcgacgga tattatcaat gacttcatcg
    16321 gtaataatgg cggactcggt aaaaatactg ccgccgtgag cgatgcggtg gccaattaag
    16381 gccacgctgt catttaaatt ccgtttttcc agttcaaatg caattgcctt caatgcacct
    16441 tcgtagctgt ggtgagccag cggtgctggc tctcccccat ttacggataa gaatgcattt
    16501 tccgagttaa taccgtcggc aatacctgac attaatactt cacaatcgct ggcatcgagt
    16561 acggaaaact taatcgaaga cgaaccacag ttaataacca aaacaaccgg aaattcattc
    16621 atctcttttc tcatcctgag ttacggatta aaacagtttg tatacgatgt tcaggatggt
    16681 cagcagacca atcacggtaa caaacacgtt atccagacga ccacggtatt tcgccagaga
    16741 cggcgcttta cggatagcat acatcggcaa caggcacagc agggatgcga taatcggtgc
    16801 gcccatggct tcaatcaggt cgaggatgtt cgggttggcg taggcaacaa cccaggtgga
    16861 gcccatgatg aagatcatgc tgagagtatt cagtttaccc agcgacactt tggttttgtc
    16921 acctttataa ccgaacttca gaatcagacc attcaagcct tccagcgtcc ccagatagtg
    16981 accgaagaaa gatttgaaga tagccacgag tgcgatgatg gaagccgcat attccagtgt
    17041 aatcgcgaac gttgttttcg taccggtcat ggacgcaaag tggttagcca gataagaaag
    17101 cactggaata ttctgcgctt tggcttccgc catgttggcc ggagacagag taaacaggca
    17161 gctaaaggca aagaacatca ccactgcaac catcagcatg ctggcacgag aaatgatttg
    17221 ggaacattta cgttcggtga agtcgcgacc gaagtctttc tcatactctt cacgtttaga
    17281 aaccacgaag gaagagacga ttggcgagaa gttaaaggag aaaaccatga tggaaatccc
    17341 cagccagaca gtgatcagga taccgtcatg accggttaac gacagcgaac cgaggtcaac
    17401 ctggtcgata actgcagagt tccagtaagg gatcagcgac agagaaatca gcaccaggct
    17461 ggcgataaac ggccatacca ggtagctcat cactttaacc atcagatcct taccaaacca
    17521 gatgacgaaa gccatcagca gcaacaggaa cagcgccaca aagccgcgat tcagcggtgc
    17581 aaatccgagc tggttttccc agaacgtcat aaaggtattg gtaatagtaa cgccataaat
    17641 ccacagcagt gggcaaatcg cgaagaagta caggaacgtg ataaccacgc cgccagtttt
    17701 accaaaatgc tcttccaccg tttccgtaat gttgccggaa gggttagagc cagaaagaca
    17761 cagacgcgcc agcgcccggt ggcaataaaa tgcgatgggg tatgccaata ccaacatcag
    17821 aagaatcggg atcagtccgc caaaacctgc gcggataggg aagaacagca ccccggcgcc
    17881 gattgccgta ccaaacaagc ctaacgtcca tgtggtatct gatttacgcc aggacgattg
    17941 ttttgtctgg ctggatacaa tgctatctga agtactcata tcctatcctc aacgaattaa
    18001 ttaagcgtca acgaaaccgg tgatttgaga gacgcgagaa agatcgatat tgccgccgga
    18061 aataatactg acggtttttc tattttgaat atattggtct aatttaccgc ttaataatgc
    18121 agcacatgcc agagcgcctg cgccttcggt gacgacttta ttgcgctgaa ttaaggcaat
    18181 catactgttt ctgatttcgt cttcgctgac cagcacgatg tcatcgacta attcacgaac
    18241 gatttcgtaa gttaaattac ccgggcggga gacatcacaa ccatccgcca gggtgccggt
    18301 agttcggtgc gtggttattt ctccggagtg gaaagaagcc gccatgccgt gaacgttttc
    18361 tgactgtacg ccaataacac gaatggtcgg gttaatagat ttaattgcca ccgcaatacc
    18421 agcaattaaa ccaccaccac caattggcac aatcacgtta tcgacatcat agagatcttc
    18481 cataatttcc agaccaatcg ttccctggcc agcaatcact ttcggatcat cgtaaggtgg
    18541 gataaaaata cgaccttcca tttcgacaat ttcgctcact ttagcgatag tgtcgttgaa
    18601 gttatcacca tgcagaacga cttctgcgga gtagtcgcac gttgccgcta ctttggattt
    18661 tggcgcacct tttggcatca ccactttacc gtcgataccc agcatcgcgc aggagagcga
    18721 aaccccttgc gcatggttgc ccgcagaaca ggccaccacg cctttgcgtt tttccgcatc
    18781 ggtcagtgaa cttaatttat taaatgcgcc acgaatttta aatgaacccg tacgctgcat
    18841 attttcaaac ttcaggaata tttcaccttt gcaacgttca ctaaaatagt tggagcgagg
    18901 catgcctgtt ttataaattc gcccagccag tcgttgtttc gcttcaataa tatcatcaat
    18961 agcaaccggc agatcgtatg taatatgcat tataaaacct cttcgcctga attaaatatg
    19021 taggtaaccg taaccgacac ctgcaaaaca gacaggtgga ttatttatgt ttaaataaaa
    19081 acaaacaact aaccaacttc tattaattgc cttcgtctac acccattata agatgaatac
    19141 tctttggcta attccaccaa aaccgatgct gcttttttaa tacgataatt tttcgaccat
    19201 accgcggcat attgtgccac aggtaatgtt tcttcaaccg gaatagtaat aaattgatta
    19261 gaaccaaaag gtgacgtcat atcacaagga attacagtta agaaatcagc attgagaaca
    19321 agattataaa ttgtcacgac tgagtcggtt ttaacgatgt tttcaatact gatgccattt
    19381 ctttgtaacg tagtaagcag ttcgctgtag taccccatat tagtttgtgg caacacccac
    19441 tgttcgttct tcaacgactc cagcgtggtg gtgccggtgc atgttcggga cttactggct
    19501 accagcacaa actcggactc gaacagcggc tcaacatgta aatcctgaag cttcatttct
    19561 gcacttaacg taccaatcgc aaaatccagt cgaccgtcgc ggattgccgg taagaacgaa
    19621 gacagttgcg cttcatacat agaaacctgc gctttcggga acacctcttt gaacttgttg
    19681 atcatccctg acataaaagt aaaaccaatc aatgaaggaa aaccaaatga gacttccacc
    19741 accgcttcag aagacatacc gcttatctca ttaaccatat ttttcatttc acgggtaatg
    19801 gattcggaac gggagagtaa caattgaccg gcaggtgtta atgttacacc ggtatttttc
    19861 cgcaccacta attccacacc aaaataatct tcaatatcgt taatgatttt actgacggcc
    19921 ggttgagtta accctaattc ttttgcagcc gagccgatag agccacttct aatgacttcc
    19981 tgaaagacta ccaggtgctg cgttttcgga agaagaatag tgctcataac gacctacgtt
    20041 aattacctca ttgacggcat gaagtgtatc aaaatgaaat aaacaggata tgtgcgacca
    20101 ctcacaaatt aactttcatt actttccaag gtatcgttat tacaattaaa catctttaaa
    20161 tatcaacaag ttaaagcata aaaatcagca taaaccctga tttttatcaa aaaaaaatga
    20221 cggggataac catattttat ggcgataaca tcattcgtta tgtggttaat acaaaaaagg
    20281 ggctgagacc atatttcaag caattaccgg ataattacca ggcaaaattt gagttaaatt
    20341 taatgagcaa gttctcaaat tttattataa ataaaccatt ttcagcgata aatactgcgg
    20401 cacgtcacat tttttcacgc tatttgttgg agaacaaaca tttattttat caatatttta
    20461 aaatttcgaa tacaggtatt gatcatctcg aacaattgat taacgtcaac tttttctctt
    20521 ctgacaggac gtcattttgt gaatgcaatc gttttccata aattcttctc ccctcatagg
    20581 cgacgaatag cattttgtgt tgaggatcac aaaacgaata attgctggtc gccgcgataa
    20641 ggtcagacga agtcaacaag ggggattttc actgtgcttt acatcagcgt aggacacaga
    20701 atgttgcttc gtttactgta acgccgggta aatgagcgtt ttttgatagt gcgtaagtgt
    20761 cgtgcgagga aaataagcat ctattattgt ttgtcatgac gacaaggcag gcatcaatga
    20821 aaggcttccc aattgcacat atttttcacc cttcaatccc gccaatgcac gcagtggtta
    20881 acaatcacaa tagaaatatt gattattgga cggtaaaaag aaagtttgca gaaattgtct
    20941 ccaccaatga cgttaataaa atttacagta taagtaatga actccggaga gtattatctg
    21001 caattactgc attgaatttc tatcaaggcg atgttccttc tgtcatgatt ccgaatccaa
    21061 ccggaaaata tgagtccatt catcatagat atttctacag gagaacatga tgattatatc
    21121 atacaaacat tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc
    21181 gtcaataaaa aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc
    21241 acccaaaaag aagccatctt aacaccccct aggcacttta ataaaacctg cattacttca
    21301 gattgttggc aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta
    21361 tttataaagg ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat
    21421 ttagtccgtc gaaaaatgaa tgaccttcct gaaagcccca taggaaataa cctcgaagca
    21481 ctagcaccag gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac
    21541 gcgttacaac caagtggaaa atgcattatc actggtgatt ttcaaaaaga attagcgtac
    21601 ttacaaaatg taattcttta taatgactcg tctcttcgtc tggatttttt aggttataac
    21661 acccaaatta ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa
    21721 aactaggaaa tatctacagg caatatcaat gttaattgcc cattaaaaga tatttacaat
    21781 gaaatcagga ggttgaacgt aatctttagt tgtgggactg gagatatagt tgatctatcc
    21841 tctctggact tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct
    21901 aatactattc taaatcattt taaattggat tcaacaaatt ttactaatgc caacatgttt
    21961 caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa
    22021 ataacgcctg ttttaataag cattagcgat atgtattctg aagaaaaaat caagtttgtc
    22081 gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt
    22141 gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca
    22201 aaagaaatat ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca
    22261 gagatgaaag aattctcatc aaaaaaaaca tatgattaca tcgatacaat cattgaagat
    22321 tatgaaaata tcaaagaaaa tgcttatctg gtcgtccccc aaattaatta tactatggat
    22381 ttaaacatag aagactctag ctcagaagag ttaataccct cgagaaagac gaaaattctc
    22441 cggacaatgg ctttgaggtc ggggaatata acacatatga agcatataac tcagagaatc
    22501 aatattttac cagagaggac tatacgtata attacgacct tttaaatgca atatagactt
    22561 aataacttct tacaaagtct attattaata ccgtagatat tcatcttacc ctgtgcacca
    22621 aaagcaaaaa cttatcgacg cgggtttgaa actgatggca accgcaaaaa tgcctgatgc
    22681 gctacgctta tcaggcctac gccatctctg caatatattg aatttgcgtg cttttgtagg
    22741 caggataagg cgttcacgca gcatccggca tgaataaagc gcactcaaca atctgaaacc
    22801 cgccggaacg gtttattacg tacatcaggt gaaactgacc gataagccgc tttcttttgg
    22861 gtatagtgtc gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa acccgccgaa
    22921 gcgggttttt acgtatatca ggtgaaactg accgataagc cgctttcttt tgggtatagc
    22981 gtcgtggaca gtcattcatc tttctgcccc tccaaaagca aaaacccgcc gaagcgggtt
    23041 tttacgtaca tcaggtgaaa ctgaccgata agccgctttc ttttgggtat agcgtcgtgg
    23101 acagtcattc atctttctgc ccctccaaaa gcaaaaaccc gccgaagcgg gtttttacgt
    23161 aaaacaggtg aaactgaccg ataagccggg ttctgtcgtg gacagtcatt catctaggcc
    23221 agcaatcgct cactggctca agcagcctac ccgggttcag tacgggccgt accttatgaa
    23281 cccctatttg gccttgctcc gggtggagtt taccgtgcca cggactgtta ccagccgcgc
    23341 ggtgcgctct taccgcaccc tttcaccctt acctgatccc gcttgcacgg gccatcggcg
    23401 gtttgctctc tgttgcactg gtcgtgggtt tcccccccag gcgttacctg gcaccctgcc
    23461 ctatggagcc cggactttcc tcccctccac ccgtctcccc cgaagaggac gacgacgaag
    23521 cggcgactgt ctggtcagct tcggcgcgca gtatagaggg tttgcgcgcc cttgtcaccc
    23581 cgtgttgcgc attccaatcg ccagtgtcgc ggcaatatta cgtgaagcac ggcagatatt
    23641 gtcataagcc ccgcggaatg cttcgtccaa cgtacctatg ctggtcaata cgctgaagac
    23701 cgcatcaatg ccatgctgat gtacaacgcc aacatcatcg gtcaggctac ccgcaatgcc
    23761 aatcaccggt ttatggtact tcttcgccac gtttgcgacg ccaatcggta ccttgccgtg
    23821 aatactctgg ctgtcaatac gcccttcacc ggtgatcacc agcgtacaat cgtgaatatg
    23881 ttcctccaga ttcagcgccg tagtgacgat ttcaatacca cttttcagtt ccgcaccaag
    23941 aaacgccatc agcgccgcac ccataccgcc cgcagctcct gcgccgggga catcttttac
    24001 atcaacatgc agcgcttttt taatgacatc ggcatagtga gagaggttat tgtccagctc
    24061 aacaatcatc gcttcactgg ctcccttttg tgggccaaag atgcgcgatg cgccgttatc
    24121 gcccaccagc ggattggtga catcacaagc gacgcgaatg acgcaatctt ttaagcgcgg
    24181 atcgaggccg gaaatatcaa tatcattcag agtattaaga ctaccgccgc caaaaccaat
    24241 ttcattgccg ttggcgtcgc ataatttcgc ccccagcgcc tgtaccatgc ctgcgccgcc
    24301 atcatttgta gcgctaccgc caatgccgat aataatgttt gtcgcgccgc tctccagcgc
    24361 ctgcaggatt aactcgcctg tgccgcgtga agtggtcacg agtggatcgc gtttttccgc
    24421 aggtaccagc tccagcccac tggccgccgc catttcaata aacgcggttt tgccatcgcc
    24481 ggagatcccc caactggcat tcactttctc gcccagcggc cctgtaaccc aggcgtgacg
    24541 ttcagaaccc tgggtggctg caatcatcgc ttccaccgtt ccttcgccac cgtcggcaac
    24601 cggaatagaa acgtactgtg catcaggaaa aatttcccga aatccttttt ctatcgcctg
    24661 cgcaacctcg ctggcagata aactttcttt ataagagtct ggggcgatta cgattttcat
    24721 acctatgcct gttaccacat gacgccggag ggcgtttctc ttattcggcc tggattccag
    24781 gcccggattg caatacgcca tccgggcacg acgtcattaa cgagtaactt cgactttcgc
    24841 cagtttttcg tagtagcaca ccagggcgct atgatccgcc gttcctaaac catctgctcg
    24901 cagtgcctgc atcatctcca taaccgcagc tgtgagcggc agttgcgcgc cgacgccgtg
    24961 ggaagtatcc agcgcatttg ccagatcctt aatatgcaga tcaatacgga agcccggctt
    25021 gaagttgcgg tccatcacca tcggcgcttt ggcatccagc acggtactgc ccgccagtcc
    25081 accgcgaatt gcctgataca ccaggtccgg gttaacgccc gctttagttg ccagcgttaa
    25141 cgcttctgac atcgcggcaa tattcagcgc cacaatgacc tgattcgcca gtttggtgac
    25201 gttacctgca ccaattgccc cggtatgcac cacggaaccc gccattgctt tcatcaaatc
    25261 atagtatttg tcgaaaatag ccttgtcgcc gcccaccatc actgacagcg taccgtcgat
    25321 ggctttcggt tcaccgccgc tcaccggagc atccagcata tcaatgcctt tcgctttcag
    25381 cgcttcgctg atttcacggc ttgccagcgg tgcgatagaa ctcatatcga tcaataccgt
    25441 acctggcttc gcgccttcaa taatgccatt ctcacccagc gccacctctt tcacatgagg
    25501 ggagtttggc agcatggtta tgatgacgtc gcactgttca gcgatcgctt tagccgtaga
    25561 ggctgtttct gcacctgcag caatcacgtc agcaatagct tctgggttac ggtcagcaac
    25621 caccagcgag taacctgctt tcagaaggtt tttactcatt ggtttaccca taatccccag
    25681 gccaataaaa ccaactttca tagtcatatc aatcatctct cttgttgcgg tggtggttat
    25741 tttttaaagg tatcagccag tttatgagtg gcagagcgga agacgccgag atcgctgccg
    25801 acagccacaa acgtcgcgcc ccattccaga taacgacgcg catcggcttc gaccggcgcg
    25861 aggataccgc tgggtttgcc gtgcgcgctg gcacggttaa aaatgtgctg aattgctttt
    25921 tgtacatccg ggtgtgatgc attgccgaga tggcctaatg ccgcggccag atcgctgggg
    25981 ccgacgaaga tgccgtctac gccttcggta gcggcaatgg catcgacgtt atctacgccc
    26041 tgctgacttt ctatctggac cagaatagtg atgttcttgt tcgactgagc gaaataatcc
    26101 gccacggtgc caaacatatt ggcgcggtga gaaacggaga cgccgcgaat gccttccggt
    26161 gggtaacggg ttgatgccac cgcctgctct gcttcctctt ttgtttctac aaaaggaatc
    26221 aggaagttat agaaaccgat atccagaaga cgcttaataa tttccggctc gttggtcggc
    26281 actcacacta ctggcgcgct ggcgctgcct ttcaaggcca ttaactgcgg aataaacgtg
    26341 gagatatcgt ttggcgcatg ttcgccatcc agcaccagcc agtcaaaccc agccaaacca
    26401 agaacttcag tgctaatcgg gttagagagt gctgaccagc aaccaatttg tacctgtttc
    26461 gcagccagtg cggctttgaa tttattcggg aaaacatcgt tattcatcgc ttataccttt
    26521 gcttatttct gcaattccat acgtttaatg tcgccaacta cgaagaggta gcagaccatc
    26581 gccatcagcg ctgaacatcc cacgaaaacc agtgctgcat tgaaggagtg cagttcactt
    26641 accaggtagc caatcaccag tggagtgaca atggaggcaa cattgccaaa gacgttaaag
    26701 acgccgccac agaggccaac aatctctttc ggcgcggtgt cagaaatcac cggccagccc
    26761 agagcaccaa atcctttgcc aaaaaaagcc agcgccatca gcatgaccac cagcgtggtg
    26821 ttgttggtgt agttacataa gatgatggtg gaagccagca acattcccag cacaatcggt
    26881 agcttacgtg ccagggtcag ggataaaccg cgtttgatca gataatccga gaagacacct
    26941 cccagcacgc cgcccgcaaa accacacagt gccggaatcg aggcgaccag acccactttc
    27001 agaatcgaca tgcctttttc ctgcaccaga taaatcggga accaggtgag gaagaaccag
    27061 gtgatggtgt tgataaaata ttgtccgaaa aatacgccca acatcatgcg gttagagagc
    27121 aattgcttga tgtaatgcag tttgggaccg cttgctgccg cgctgcccgg ctttttgtgg
    27181 tccatatcga ccaccgcgcc attttcagag ataaacttca gctcttcctc agacatacgt
    27241 gggtgatctg tcgggttatg aatcaacttg atccacagcg ccgtcagcac aaaacaaatc
    27301 acccccataa cggtaaagac gtgctcccat ccccaggcga aagtcagcca gccaagcaga
    27361 ggcgaaaaga gcgccagcga gaaatattgt gccgagttaa agatggcgga ggcagtacca
    27421 cgttctttcg tcgggaacca ggcggcgaca atacgggcgt tcgccgggaa ggatggagct
    27481 tccgagaatc ccagcataaa gcgcataaag aacatggaga tccctgccca agccagcggg
    27541 aacatatcaa caaagccttg caggaaggtg aacagcgacc agaaaaagag gctgtaggtg
    27601 taaacttttt tcgagccaaa cttatcaagc aaccagccgc cggggatttg catcagcaag
    27661 taggcccagc caaaagcgga gaagatgtaa cccatcgaaa ccgcacttaa ctgcaactct
    27721 tttgccactt cggtaccagc aatagacagc gttgcacgat cggcgtagtt aacggcggta
    27781 acaataaaaa taatcagtaa tattaaatag cgggtatgca cacctttcct tttttcgtca
    27841 acggtgtcca gaatcatttt atttacctcg ggtacttatg ctgattttta ttattatggg
    27901 gaaggtgtta tttataagtt tcatttatgc cgtaacgaca atgaactcgg gaattagtat
    27961 aagcagcgcg agaataataa tcattgtgca aatgctaatt taattaatac tttttaaata
    28021 ttattttgag catatgcaca taaggttgcg cgctaaagca cagatttgcg ctttaccttg
    28081 ccgggcggca ctgcaatccc taaaatgatt gacattgatc acatttctgc gtttaaactc
    28141 ctgacattct tatttcaccc aatgaaatca tttattttta aatgagacca ggtcctcatt
    28201 ttaataaccc ctggctggag aatattgcac aatggccaac atcgaaatca gacaagaaac
    28261 gccaactgcg ttttatataa aagttcacga cacagataat gtggcaatta ttgttaatga
    28321 taatggcctg aaagcaggaa cgcgttttcc ggatgggctg gaattaattg aacatattcc
    28381 ccaggggcat aaagtcgcat tgctggacat tccggctaat ggtgaaatta ttcgttatgg
    28441 cgaagtgatt ggttacgccg tgcgtgcaat cccacgcgga agctggatcg acgaatcaat
    28501 ggttgtacta ccggaagcgc cgccgttaca cacgctgcca ctggcaacca aagtcccgga
    28561 acccttaccg ccgctggaag gatacacctt tgagggctat cgcaatgccg atggcagcgt
    28621 gggcaccaaa aacctgctcg gcatcaccac cagcgtccac tgtgtggcag gtgtggtgga
    28681 ctatgtagta aaaatcattg aacgcgatct gctaccgaaa tacccgaacg tcgatggcgt
    28741 ggtggggctg aatcatttgt acggttgtgg cgtggcgatt aacgcaccgg cagcagttgt
    28801 acctattcgt accattcaca atatttcgct gaaccctaac tttggcggcg aagtaatggt
    28861 gattggcctg ggttgtgaaa agttgcagcc tgagcgcctg ctgactggaa cggatgatgt
    28921 gcaagctatt ccagtagaaa gcgccagcat tgtcagtttg caggatgaaa agcatgtcgg
    28981 ttttcagtcc atggtcgagg atattttgca ggtcgccgaa cgccatctac aaaaactgaa
    29041 tcaacgtcag cgagaaacct gtccggcttc agaactggtt gtcggcatgc attgcggtgg
    29101 cagcgatgcg ttttctggcg taacggcaaa cccggcggtt ggctatgcgt ctgatctact
    29161 ggtgcgctgc ggcgcaacgg tgatgttttc agaagtcacg gaagtgcgtg acgcgatcca
    29221 tctgctgaca ccacgcgcag tgaacgaaga ggtcggcaaa cggctgctgg aggagatgga
    29281 gtggtacgat aactatctca atatgggaaa aaccgaccgc agcgccaacc cttcgccggg
    29341 caacaagaaa ggcggtctgg caaacgtggt agagaaagcg ctcggctcca ttgctaaatc
    29401 gggcaaaagt gcaattgttg aagtgctgtc gcccggtcaa cgcccgacta aacgcggatt
    29461 aatttacgcc gcgacgccag ccagcgattt tgtctgtggc acgcaacagg tggcttcggg
    29521 tatcaccgtg caagtgttta cgaccggtcg tggtacgccg tacggcctga tggcggtacc
    29581 cgtcattaaa atggcacccc gcaccgagct ggcgaaccgc tggtttgatt taatggatat
    29641 taatgcgggc accatcgcta ccggcgaaga aactattgaa gaggagggct ggaagttgtt
    29701 ccactttatt ctcgacgtcg ccagcgggaa gaagaaaacc ttctcggatc aatggggact
    29761 gcataaccag ctggcggtgt ttaacccagc accggtgacc tgataattca attcgtatta
    29821 tgcccgatag cacacgttta tcgggcatat tgagtcacag cgaagacgcg tgatccccga
    29881 ccagaatcac ttcaacccca gcctttcgca gtccttccag actatccgca ggaatgcctt
    29941 catcaacaat gatcatgtcg atacgttgag tatcaatgat cttatgtaaa ctggaacgat
    30001 tgaatttact ggaatcggtg accacgatga tccgttccgc aacttcgcac atccgacggc
    30061 ttaaacgggc ttcatcttca ttatgtgtgc tgacgccgcg ctccagatcg atcgcatcta
    30121 caccaagaaa cagcatatcg aagtggtaat tttgcagcga ttgctcagcc tgatcgccgt
    30181 aaaaagattg cgactgacgg cgcaaatgcc cgccggtcat cagcagctca acgccttccg
    30241 cttccagcaa cgcattagcc acgttcatac cgttggtcat cgcaattacg tcagtgtact
    30301 tgcgcatcag acgagcaatc tcaaaagtgg tggtcccgga atcgaggatc acccgatgac
    30361 ctggctgaat caactcaacg gcagctttcg caacgctgcg tttcatcgcg gtgttcagtg
    30421 cgcttttatc ttccactgat ggctcgactg acggcgtcgt gctatcgcag atcaacgcgc
    30481 caccataggc acgcacagcg atcccctgct tttccagaaa cgccagatca ttgcggatcg
    30541 tcacagtaga tacgccatac aatgccgaca gatcgttaac ctgcacactc ccttgctgtc
    30601 gcagacgctg aatgatctgt tctcgtcgct cgctggtgcc tgtcactcgc ttctcacctg
    30661 aagcgtcggt attactcata gtaagtcctt tcgtaaaact ttcgtttcat ttcgttttgc
    30721 ctattaacgc ctttctatta agcaaatgca agcccacctt gcccattggc gcaagctact
    30781 ctcgtttcag tgactttcat tacgtttctt ttgtgaatca gatcagaaaa ctattatctt
    30841 tcgttttatt tttatctcac catgacgcag tatcaattga aacaaaacga aagatcaata
    30901 tcgcagtaat ctgaactgga gaggaaagtg aaacatctga cagaaatggt gagacagcac
    30961 aaagcgggca aaacaaatgc aatttatgcc gtttgttccg cacatccgct ggtgctggag
    31021 gctgcaatcc gctacgccag tgcaaaccaa acgccgttac tgattgaagc aacctccaat
    31081 caggtagacc agttcggcgg ttataccgga atgacgcccg ccgattttcg cggctttgtt
    31141 tgtcagctcg ccgactcgtt gaatttcccg caggatgcgc tgattctggg tggtgaccat
    31201 ctggggccaa accgctggca aaacctgcca gccgctcagg caatggccaa tgccgatgat
    31261 ttgattaaaa gctacgttgc ggcaggattc aaaaaaatcc accttgattg cagcatgtcc
    31321 tgtcaggacg atccaattcc cttaactgat gacatcgtgg ctgaacgcgc cgcccgtctg
    31381 gcgaaagtgg cggaagaaac ctgtcgcgaa cactttggcg aagccgatct ggagtatgtc
    31441 attggtaccg aagtgccggt acctggcggc gcgcatgaaa ccttaagcga gctggcggtc
    31501 accacgccgg atgccgcccg cgccacgctg gaagcccatc gtcacgcctt tgaaaagcaa
    31561 ggtttgaatg ccatctggcc acgcattatt gccctggtgg ttcaacccgg cgtcgaattc
    31621 gatcacacca acgttattga ttatcagccc gccaaagcga gcgccttaag ccagatggtc
    31681 gaaaactacg aaacgctgat tttcgaagcg cactctaccg attatcaaac gccgcaatct
    31741 ctgcgccagc tggtgattga ccactttgcc attctgaaag ttggcccggc gctgaccttc
    31801 gccctgcgtg aagccctttt ttctctggcg gcgattgaag aagaactggt accagcgaaa
    31861 gcctgttccg gtctgcgtca ggtgctggaa aacgtgatgc tcgaccgccc ggaatactgg
    31921 caaagccact accacggtga cggcaacgcg cgtcgtctgg cgcgtggtta cagctactca
    31981 gatcgcgtgc gctattactg gccggacagc cagattgatg acgctttcgc tcatctggtt
    32041 cgtaatctgg cggattcacc aattccgcta ccgctcatca gccagtatct gccgctgcag
    32101 tacgtgaaag ttcgctccgg cgagctgcaa ccgacgccac gggaactcat tataaaccat
    32161 attcaggaca tcctggcgca gtaccacaca gcctgtgaag gccaataagc aaaacaaaga
    32221 ggaacatgct atgccaaata ttgttttaag ccggattgat gaacgcttga ttcacggtca
    32281 ggtcggcgtt cagtgggtcg gatttgcggg ggcaaatctg gtgctggtag ccaacgatga
    32341 ggttgccgaa gatccggtac aacaaaacct gatggaaatg gtactggcag aagggatcgc
    32401 cgtacgtttc tggacgctgc aaaaagttat cgacaacatt catcgcgccg ccgatcgaca
    32461 gaaaatcctg ctggtttgta aaacacccgc cgatttcctg acgctggtga aaggtggcgt
    32521 tccggtgaat cgcattaacg ttggcaatat gcactacgcc aatggcaaac aacaaatcgc
    32581 caaaacggtt tctgtggatg cgggcgatat cgcagcattt aacgacctga aagccgctgg
    32641 ggtggaatgc ttcgttcagg gcgtcccgac agagcctgct gtggacctct ttaaattact
    32701 ttgagggatt catcatggaa atcagcctgt tgcaggcatt tgcgttgggc attatcgcct
    32761 ttatcgctgg cctagatatg tttaacggct taacccatat gcaccgcccg gtggtcctcg
    32821 gcccgttggt cgggctggta cttggcgatc tgcataccgg aattttaacc ggcgatacgc
    32881 tggaactagt gtggatgggg ctggccccgc tggcgggcgc acagccgcct aacgtgatta
    32941 tcggtactat cgtcggcacg gcgtttgcca ttactactgg cgtgaaaccc gatgtcgcgg
    33001 tcggtgtcgc cgtacctttc gctgtcgcag tacagatggg gattaccttc ctgttctcgg
    33061 tgatgtctgg cgtgatgtct cgctgcgacc ggatggcgga gaacgccgat actcgcggca
    33121 ttgaacgcgt gaactatctg gcactgctgg cactcggcac tttctatttt ctctgcgctt
    33181 tcctgcctat ctacttcggc gcggaacatg caaaaaccat cattgatgtc ctgccgcaac
    33241 gattaatcga cggcctcggc gtcgcgggcg gcatcatgcc agcaatcggt tttgccgtgc
    33301 tgctgaaaat catgatgaaa aacgtctaca taccctactt catcctgggc tttgttgccg
    33361 caacctggct caagttaccg gtgctggcta ttgctgccgc cgcgctggcg atggcgctga
    33421 tcgatctgct gcgtaaatct cctgaaccga ctcaacctgc ggcacagaaa gaggaatttg
    33481 aagatggcat ctaatcaaac caccctgccg aacgtcagcg aaaatgaaga aacactgctg
    33541 actggcgtca atgaaaacgt gtatgaagat cggagcattg ccgcggagct gacgaaaaaa
    33601 gatatcaacc gcgtcgcctg gcgttccatg ctgttacaag cgtcttttaa ctacgaacgt
    33661 atgcaggctt ctggctggtt gtacggtctg ctgcctgctc tgaaaaagat ccacactaat
    33721 aaacgcgacc tggcgcgcgc catgaagggg catatgggtt tcttcaatac ccatccgttt
    33781 ctggtgacgt ttgttatcgg cattatcctt gcgatggagc gttctaagca ggacgttaac
    33841 agtattcaga gcaccaaaat tgccgtcggt gcgccgcttg gcgggattgg cgatgcgatg
    33901 ttctggctaa cgctactgcc gatttgtggc gggataggcg caagcctcgc gctacaaggc
    33961 tctattcttg gcgcagtcgt ctttattgtg ctgttcaacg tggtacactt ggggctgcgt
    34021 tttggtctgg cgcattatgc ttaccgcatg ggcgtggcgg cgattcccct tattaaagcg
    34081 aataccaaaa aagtcggcca tgctgcgtca atcgttggaa tgacggtaat cggcgcactg
    34141 gtggcaacct atgttcgttt aagcaccacg ctggaaatca cggcgggcga cgcagtagtt
    34201 aagttacagg ctgatgttat cgacaaactg atgcctgcct tcttaccgct ggtctacacc
    34261 ctgaccatgt tctggctggt acgccgcggc tagagtccgc tgcgcctgat tgcggttacc
    34321 gtggttctag ggatcgtcgg taaattctgc catttccttt aaatacaatg aggtattaga
    34381 tgttaagtat tattttgaca gggcatggcg ggtttgccag tggcatggaa aaagcaatga
    34441 agcaaatcct cggcgatcaa tcgcagttta ttgccatcga ttttcccgaa acctcaagcg
    34501 ccgcgctgct tacatcgcag cttgaagagg ccatcgccca actggattgt gaagacggca
    34561 ttgtttttct gacggatttg ctgggcggca cgccgtttcg cgtagcttca acgctggcga
    34621 tgcaaaaacc gggctgtgaa gtgattaccg gcaccaattt gcaactgcta ctggagatgg
    34681 tgctggagcg cgaagggtta agcggtgaag aattccgtgt acaggcgctg gagtgtggac
    34741 accgagggct gaccagtctg gtggacgagt taggccgctg ccatgaagaa tgtccggtcg
    34801 aggaaggaat atgacacacg ttctgcgcgc cagaaggctg ctgactgaag agggatggtt
    34861 cgatgaccat cagttgcgtg ttgctgacgg tgtcatcgca gcaatcgaac cgattccagt
    34921 gggcgtgact gaacgcgatg cggaacggct ctgccccgct tacatcgaca cccatgtaca
    34981 cggtggtgcg ggcgttgatg taatggatga cgcgccggat gtactcgaca agctggcaat
    35041 gcacaaggca cgcgaaggtg tcggcagttg gttaccgacc accgtaaccg cgccgcttaa
    35101 taccattcat gcggcgctga aacgtattgc tcaacgttgc caacgcggcg gacctggtgc
    35161 gcaagtgctg gggagttatc tcgaaggacc gtacttcacg ccgcagaata aaggcgcgca
    35221 tccg
//
LOCUS       DAETQL010000037        35016 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.037, whole genome shotgun sequence.
ACCESSION   DAETQL010000037 DAETQL010000000
VERSION     DAETQL010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35016
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.037"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..835)
                     /locus_tag="K0W65_001975"
     CDS             complement(<1..835)
                     /locus_tag="K0W65_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBI3095248.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDYH
                     GP"
     gene            927..1013
                     /locus_tag="K0W65_001976"
                     /pseudo
     CDS             927..1013
                     /locus_tag="K0W65_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1418..1894)
                     /locus_tag="K0W65_001977"
     CDS             complement(1418..1894)
                     /locus_tag="K0W65_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBI3095249.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1953..3242)
                     /gene="bioA"
                     /locus_tag="K0W65_001978"
     CDS             complement(1953..3242)
                     /gene="bioA"
                     /locus_tag="K0W65_001978"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBI3095250.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3329..4369
                     /gene="bioB"
                     /locus_tag="K0W65_001979"
     CDS             3329..4369
                     /gene="bioB"
                     /locus_tag="K0W65_001979"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBI3095251.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4366..5520
                     /gene="bioF"
                     /locus_tag="K0W65_001980"
     CDS             4366..5520
                     /gene="bioF"
                     /locus_tag="K0W65_001980"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBI3095252.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5507..6262
                     /gene="bioC"
                     /locus_tag="K0W65_001981"
     CDS             5507..6262
                     /gene="bioC"
                     /locus_tag="K0W65_001981"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBI3095253.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            6255..6932
                     /gene="bioD"
                     /locus_tag="K0W65_001982"
     CDS             6255..6932
                     /gene="bioD"
                     /locus_tag="K0W65_001982"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBI3095254.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7511..9532
                     /gene="uvrB"
                     /locus_tag="K0W65_001983"
     CDS             7511..9532
                     /gene="uvrB"
                     /locus_tag="K0W65_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBI3095255.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9724..10632)
                     /gene="yvcK"
                     /locus_tag="K0W65_001984"
     CDS             complement(9724..10632)
                     /gene="yvcK"
                     /locus_tag="K0W65_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBI3095256.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10897..11041
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            11029..12018
                     /gene="moaA"
                     /locus_tag="K0W65_001985"
     CDS             11029..12018
                     /gene="moaA"
                     /locus_tag="K0W65_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBI3095257.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            12040..12552
                     /gene="moaB"
                     /locus_tag="K0W65_001986"
     CDS             12040..12552
                     /gene="moaB"
                     /locus_tag="K0W65_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBI3095258.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12555..13040
                     /gene="moaC"
                     /locus_tag="K0W65_001987"
     CDS             12555..13040
                     /gene="moaC"
                     /locus_tag="K0W65_001987"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBI3095259.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            13033..13278
                     /gene="moaD"
                     /locus_tag="K0W65_001988"
     CDS             13033..13278
                     /gene="moaD"
                     /locus_tag="K0W65_001988"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBI3095260.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13280..13732
                     /gene="moaE"
                     /locus_tag="K0W65_001989"
     CDS             13280..13732
                     /gene="moaE"
                     /locus_tag="K0W65_001989"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBI3095261.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13869..14573
                     /locus_tag="K0W65_001990"
     CDS             13869..14573
                     /locus_tag="K0W65_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBI3095262.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14778..15491
                     /locus_tag="K0W65_001991"
     CDS             14778..15491
                     /locus_tag="K0W65_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBI3095263.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15527..16483)
                     /locus_tag="K0W65_001992"
     CDS             complement(15527..16483)
                     /locus_tag="K0W65_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HBI3095264.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16483..17724)
                     /gene="clsB"
                     /locus_tag="K0W65_001993"
     CDS             complement(16483..17724)
                     /gene="clsB"
                     /locus_tag="K0W65_001993"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBI3095265.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPRQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17721..18482)
                     /locus_tag="K0W65_001994"
     CDS             complement(17721..18482)
                     /locus_tag="K0W65_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBI3095266.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18615..19025
                     /locus_tag="K0W65_001995"
     CDS             18615..19025
                     /locus_tag="K0W65_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HBI3095267.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18987..20093)
                     /locus_tag="K0W65_001996"
     CDS             complement(18987..20093)
                     /locus_tag="K0W65_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3095268.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(20104..21237)
                     /locus_tag="K0W65_001997"
     CDS             complement(20104..21237)
                     /locus_tag="K0W65_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3095269.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(21230..22966)
                     /locus_tag="K0W65_001998"
     CDS             complement(21230..22966)
                     /locus_tag="K0W65_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3095270.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22959..23954)
                     /gene="hlyD"
                     /locus_tag="K0W65_001999"
     CDS             complement(22959..23954)
                     /gene="hlyD"
                     /locus_tag="K0W65_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBI3095271.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23957..24628)
                     /gene="cecR"
                     /locus_tag="K0W65_002000"
     CDS             complement(23957..24628)
                     /gene="cecR"
                     /locus_tag="K0W65_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBI3095272.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24857..26044
                     /gene="rhlE"
                     /locus_tag="K0W65_002001"
                     /pseudo
     CDS             24857..26044
                     /gene="rhlE"
                     /locus_tag="K0W65_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(26251..26733)
                     /gene="ybiA"
                     /locus_tag="K0W65_002002"
     CDS             complement(26251..26733)
                     /gene="ybiA"
                     /locus_tag="K0W65_002002"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HBI3095273.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26853..29003
                     /gene="dinG"
                     /locus_tag="K0W65_002003"
     CDS             26853..29003
                     /gene="dinG"
                     /locus_tag="K0W65_002003"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBI3095274.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            29031..29993
                     /gene="ybiB"
                     /locus_tag="K0W65_002004"
     CDS             29031..29993
                     /gene="ybiB"
                     /locus_tag="K0W65_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBI3095275.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30134..31219
                     /gene="hcxB"
                     /locus_tag="K0W65_002005"
     CDS             30134..31219
                     /gene="hcxB"
                     /locus_tag="K0W65_002005"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HBI3095276.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31448..31708)
                     /locus_tag="K0W65_002006"
     CDS             complement(31448..31708)
                     /locus_tag="K0W65_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBI3095277.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31973..32239)
                     /gene="ybiI"
                     /locus_tag="K0W65_002007"
     CDS             complement(31973..32239)
                     /gene="ybiI"
                     /locus_tag="K0W65_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HBI3095278.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPSVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32313..32990)
                     /gene="ybiX"
                     /locus_tag="K0W65_002008"
     CDS             complement(32313..32990)
                     /gene="ybiX"
                     /locus_tag="K0W65_002008"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBI3095279.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(33032..35016)
                     /locus_tag="K0W65_002009"
                     /pseudo
     CDS             complement(33032..35016)
                     /locus_tag="K0W65_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8295 a         8974 c         9464 g         8283 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg
       61 acaatggatt gttataaata ttaacatctg cataaatgtg cagattcatg atactgtctg
      121 gcaatgtgtt cagctgattg ttagggatat atagtgattg cagacgttgt ggaagttcgg
      181 gcagtctgtg tagttggttt tccccaacgt ttaaaagcct cagattttga ggaaggggcg
      241 gtagctcttg taatctgtta tcaaaagcac ttagttcctg tagttcacat ggtaattctg
      301 gtaaggcagt aagctggttg ttgccaacag aaagtttttg tagagccggg ggaagatcag
      361 gtagcgtctc caggctattg agaagggctg agagtgactg caaggaagat ggaagagaag
      421 gcaaacatgt taaaactctg ttaagtgaaa catcaagagc gaccaggtga ggcggaagag
      481 ccgggagcct gctcagtctg ttatcgctgg ctttaagtac agttaaggac ggagggagtt
      541 ctggcagaga gcgtagctca ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt
      601 atgggagaga gcgtaatctg agtaaactta aattgagggc tggctcttga aaagccagac
      661 atattttcag taatcgaact gcctgtgttc ggtcttctgt tgcagcacct tccttggccc
      721 agttatccca gatgcggtga taatccgtga tattttgctc ttctacggat aaacgggata
      781 tacatcctaa accacaaaag gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt
      841 attgatcctc attgtttata tattaatata ttcgtgcaaa tggagtgagg attctttaaa
      901 aaaacgcgcc tgtaaatagt taagaacagg aagaactgtt ggggatgtgg ctggccgaaa
      961 atgaaggggt gaagttctgg ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc
     1021 cctacctttt catcaagcca gtagtctgcc caccacagca tcatttctct gcgcttatcg
     1081 agatactgag catggttgta aattcagcta acccagtgcg ttgacaaaaa attagcgcaa
     1141 gaggacaaaa aatcaccttg cgctaatgct ctgtatcagg tcactaatac catctaagtg
     1201 gttgattcat agtgactgga tatgttgtgt tttgcagtat tatgcagtct attttttaag
     1261 ctaaatgtat tataatgcat tgatattaat gatttttact gtttctcggt cagctttttt
     1321 atactaactt gagcgaaacg ggaaggtaaa aagacaaaaa gttgttttta atacctttaa
     1381 gtgataccag atggcattgc gccatctggc agagtgatta actaaacatc gcggtaatcg
     1441 atgcgcttgc cagagagtgg aaatgaacgt taaacccgac catcgcgccg ctggctcctt
     1501 catcgacatc aatacgctct acatccagcg cgtgaacggt aaaaatgtag cgatgggttt
     1561 cgcctttcgg cggcgctgcg ccatcgtatc cggttttacc aaaatcggta cgcgtctgca
     1621 aaacgccgtc tggcattgct accagaccag agccaaaccc ttgcggtaat acgcgggtat
     1681 cagcgggtaa attaacaact acccagtgcc accagccgga gccggttggc gcatccgggt
     1741 cgtagcaggt gacaacaaaa cttttcgttc ccgcaggaac atcatcccac gccagatgcg
     1801 gtgaaatatt atcgccatcg taacccatgc cgttaaagac atgacgatgc ggcaacttat
     1861 cgccatcgcg cagatcgtta ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa
     1921 gtgtagccag aaaccctcac gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt
     1981 accgcgcggt taaccgctgc ggtcagacgc tgcaactgtt gcgggagaat aatatagggc
     2041 ggcatcaggt aaatcagttt gccaaaaggc cggatccata ccccctgttc gacaaagaat
     2101 ttttgcagcg ccgccatatt caccggacga gtggtttcga ccacaccgat tgcccccagt
     2161 acgcgcacat cggcaaccat ttcggcatca cgggctggtg ccagttgctc gcgcagctgc
     2221 acttcaatag ccgccacctg ctgctgccat tcgccggatt cgataatcgc taggctggcg
     2281 tttgctgccg cgcaggccag cggattgccc ataaaagttg gcccatgcat aaagcagccg
     2341 gcttcgccgt tactgatggt ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc
     2401 attgtgccgc cggttaaggc tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat
     2461 tcacaggcaa acagtttgcc ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc
     2521 aagatacctt cgcgatcgca cattttgcgg attcgtttta accattccgg atggtacatg
     2581 cgcatcccgc ctgcgccctg gacaatcggc tcaatgatca ccgctgcgat ttcatgacga
     2641 tgcgccgcca tcaggcgggc aaaaccaacc atatcgcgtt catcccattc accatccatg
     2701 cggctttgcg gggcgggagc aaacaagttt tctggcagat agcctttcca caggctgtgc
     2761 attgagttgt ccggatcgca caccgacatc gcgccaaagg tatcgccatg ataaccattg
     2821 cggaaggtca gaaaacgctg gcgcgcttcg cctttggctt gccagtactg caacgccatt
     2881 ttcatcgcca cttccaccgc tacggaaccg gagtccgcga gaaaaacgca ctccagcggt
     2941 tgcggcgtca ttgccaccag tttgcggcat agctcgatgg cgggcgcatg ggtgataccg
     3001 ccaaacatca catgcgacat ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg
     3061 tgattgtagc cgtggatcgc cgcccaccag gacgacatac cgtcaaccag gcgtctgccg
     3121 tcagacaaaa ttagctcgca accttcggcg ctcgccaccg gataaaccgg cagaggggag
     3181 gtcatggatg tgtatgggtg ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc
     3241 ataatcgact tgtaaaccaa attgaaaaga tttaggttta caagcctaca ccgaattaac
     3301 aacaaaaaac acgttttgga gaagccccat ggctcaccgc ccacgctgga cattgtcgca
     3361 agtcacagaa ttatttgaaa aaccgttgct ggatctgctg tttgaagcgc agcaggtgca
     3421 tcgccagcat ttcgaacctc gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg
     3481 agcttgtccg gaagattgca aatactgccc gcaaagctcg cgctacaaaa ccgggctgga
     3541 agccgagcgg ttgatggaag ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc
     3601 aggatcgacg cgcttctgta tgggcgcggc atggaagaat ccccacgaac gcgatatgcc
     3661 gtacctggaa caaatggtgc agggggtaaa agcgatgggg ctggaggcgt gtatgacgct
     3721 gggcacgttg agtgaatctc aggcgcagcg cctcgcgaac gccgggctgg attactacaa
     3781 ccacaacctg gacacctcgc cggagtttta cggcaatatc atcaccacac gcacttatca
     3841 ggaacgcctc gatacgctgg aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg
     3901 cattgtgggc ttaggcgaaa cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa
     3961 cctgccgacg ccgccggaaa gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc
     4021 gcttgccgat aacgatgacg ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg
     4081 gatcatgatg ccgacctctt acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca
     4141 gactcaggcg atgtgcttta tggcaggcgc aaactcgatt ttctacggtt gcaaactgct
     4201 gaccacgccg aatccggaag aagataaaga cctgcaactg ttccgcaaac tggggctaaa
     4261 tccgcagcaa actgccgtgc tggcagggga taacgaacaa cagcaacgtc ttggacaggc
     4321 gctgatgacc ccggacaccg acgaatatta caacgcggca gcattatgag ctggcaggag
     4381 aaaatcaacg cggcgctcga tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg
     4441 gtggcgcaag gagccggacg ctggctggtg gcggatgatc gccagtatct gaacttttcc
     4501 agtaacgatt atttaggttt aagccatcat ccgcaaatta tccgtgcctg gaagcagggg
     4561 gcggagcaat ttggcatcgg tagcggcggc tccggtcacg tcagcggtta tagcgtggtg
     4621 catcaggcgc tggaagaaga actggccgag tggcttggct attcgcgggc gctgctgttt
     4681 atctctggtt ttgccgcgaa tcaggcagtc attgccgcga tgatggcgaa agaggaccgt
     4741 attgttgccg accggcttag ccatgcctca ttgctggagg ctgccagttt aagcccgtcg
     4801 cagcttcgcc gttttactca taacgatgtc gctcatctgg cgcgactgct tgcttccccc
     4861 tgtccggggc agcaactggt ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg
     4921 ccactggcgg aaatccagca ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat
     4981 gcccacggca cgggcgttat cggggagcag gggcgcggca gctgctggct gcaaaaggta
     5041 aaaccagaat tgctggtagt gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg
     5101 ctttgctcaa gtacggtggc ggattatctg ctgcaattcg cccgccacct tatctacagc
     5161 accagtatgc cgcccgctca ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt
     5221 gatgaggggg atgcacggcg cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga
     5281 gtacaggatt tgccgtttac gctggctgat tcatgcagcg ccatccagcc attgatcgtc
     5341 ggtgataaca gccgtgcgtt acaactggca gaaaaactgc gccagcaagg ctgctgggtc
     5401 acggcgattc gcccgccaac cgtacccgct ggtactgcgc gactgcgctt aacgctaacc
     5461 gctgcgcatg aaatgcagga tatcgaccgt ctgctggagg tgctgcatgg caacggttaa
     5521 taaacaagcc attgcagcgg catttggtcg ggcagccgca cactatgagc aacatgcaga
     5581 tctacagcgc cagagtgctg acgccttact ggcaatgctt ccacagcgta aatacaccca
     5641 cgtactggac gcgggttgtg gacctggctg gatgagccgc cactggcggg aacgtcacgc
     5701 gcaggtgacg gcgttagatc tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc
     5761 cgcagatcat tatctggcag gagatatcga atccctgccg ttagcgactg cgacgttcga
     5821 tcttgcatgg agcaatctcg cggtgcagtg gtgcggtaat ttatccacgg cactccgcga
     5881 gctgtatcgg gtggtgcgcc ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc
     5941 gttacccgaa ctgcatcagg cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt
     6001 tttaccgcca gatgaaatcg aagagtcgct gaacggcgtg cgttatcaac atcatattca
     6061 gctcatcaca ctatggtttg atgatgcgct cagtgccatg cgttcgctga aaggcatcgg
     6121 tgctacgcat cttcatgaag ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg
     6181 attgcaactg gcctggccgc aacaacaggg gcgatatcct ctgacgtatc atcttttttt
     6241 gggagtgatt gctcgtgagt aaacgttatt ttgtcaccgg aacggatacc gaagtgggga
     6301 aaaccgtcgc cagttgtgca cttttacaag ccgcgaaggc agtaggttat cggacggcag
     6361 gttataaacc ggtcgcctct ggcagcgaaa agaccccgga aggcttacgt aatagtgacg
     6421 cgctggcgtt acagcacaac agcagcctgc agctggatta cgcaacggta aatccttaca
     6481 ccttcgcaga atccacctcg ccgcacatca tcagcgccca agagggcagg ccgatagaat
     6541 cattggtaat gagcgccgga ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg
     6601 aaggtgctgg cggctggttt acgccgcttt ctgacacttt cacttttgca gattgggtaa
     6661 cacaggaaca actgccggtg atactggtag ttggtgtgaa actcggctgt attaatcacg
     6721 cgatgttgac tgcgcaggca atacaacacg ccggactgac tctggcgggt tgggtggcga
     6781 acgatgttac gcctccggga aaacgtcacg ctgaatatat gaccacgctc acccgcatga
     6841 ttcccgcgcc gctgctggga gagatcccct ggcttgcaga aaatccagaa aatgcggcaa
     6901 ccggaaagta cataaacctt gccttgttgt agccattctg tatttggtta aattgcgagc
     6961 gagatcgcgt cttcgattga ctgcaattta accaattaaa ttctaaaata atcacgaaaa
     7021 aaattttact tccgcctcat gcggcgaatg tgggaattgc ccaggcggcg ggggataggg
     7081 gctggagaca gttatccact attcctgtgg ataaccatgt gtattagagt tagaaaacac
     7141 gaggcaagcg agagaatacg cggcttgcac gcgaattggc gttaaagacg gctcaaagaa
     7201 atatctttta ttttttaact ggttagataa atgcaatggc agtcactgaa caggcatctc
     7261 ttgccataaa actgtcatca ctcatcttga caaatgttaa aaaagccgtt gctttgggga
     7321 taacccggta aggctggagt tttatctcgc cacagagtaa attttgctca tgattgacag
     7381 cggagtttac gctgtatcag aaatattatg gtgatgaact gtttttttat ccagtataat
     7441 ttgttggcat aattaagtac gacgagtaaa attacatacc tgcccgccca actccttcag
     7501 gtagcgactc atgagtaaac cgttcaaact gaattccgct tttaaacctt ctggcgatca
     7561 gccagaggcg attcgacgtc tcgaagaggg gctggaagat ggcctggcgc accagacgtt
     7621 acttggcgtg actggctccg ggaaaacctt caccattgcc aatgtcattg ctgaccttca
     7681 gcgcccaacc atggtacttg cgcccaacaa aacgctggcg gcccagctgt atggcgaaat
     7741 gaaagagttc ttcccggaaa acgcggtgga gtatttcgtc tcctactacg actactatca
     7801 gccggaagcc tatgtaccga gttccgacac cttcattgag aaagatgcct cggttaacga
     7861 acatattgag cagatgcgtt tgtccgccac caaagcgatg ctggagcggc gtgatgtgat
     7921 tgtagtggcg tctgtttccg cgatttatgg tctgggcgat cctgatttat atctcaagat
     7981 gatgctccat ctcacggtcg gtatgattat cgatcagcgc gcgattctgc gccgactggc
     8041 ggagctgcaa tacgctcgta atgatcaagc attccagcgt ggtactttcc gcgttcgtgg
     8101 cgaggtgata gatatcttcc cggcagaatc ggatgacatt gcacttcgcg tggaactgtt
     8161 tgacgaggaa gtggaacgat tgtcgttatt tgacccgctg accgggcaga ttgtttccac
     8221 tattccacgt tttaccatct acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt
     8281 acaggcgatg gaggagatca aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa
     8341 caacaaactg ctggaagagc agcggctgac ccagcgtacc cagtttgatc tggagatgat
     8401 ggacgagctg ggctactgtt cggggattga aaactactcg cgcttcctct ccggtcgtgg
     8461 accgggtgag ccaccgccga cgctgtttga ttacctgcct gccgatgggc tgctggtcgt
     8521 cgatgaatct cacgtcacca ttccacaaat tggcggcatg tatcgcggtg accgggcgcg
     8581 taaagagaca ctggtggagt acggcttccg cctgccatca gcgctggata accgtccgct
     8641 taagtttgaa gagttcgaag cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg
     8701 taattacgag ctggaaaaat ccggcggcga tgtggtggat caggtggtgc gtccaaccgg
     8761 attgcttgac ccgattatcg aagtgcggcc ggtggcgaca caggttgatg atcttctttc
     8821 ggagattcgt cagcgagcgg caattaacga acgcgtgctg gtcaccacac tgaccaagcg
     8881 aatggcggaa gatcttaccg aatatctcga agaacacggt gagcgcgtgc gttatcttca
     8941 ctcagatatc gacaccgtcg agcgtatgga gattatccgc gacttgcgtc tgggtgagtt
     9001 cgacgtattg gtagggatca acttactgcg tgaaggtctg gatatgccgg aagtgtcgct
     9061 ggtggcgatc ctcgacgctg acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca
     9121 gaccattggt cgtgcggcac gtaacgttaa cggtaaagcg attctctacg gcgataggag
     9181 cactccatca atggcgaaag cgattggcga aaccgaacgt cgccgtgaga aacagcagaa
     9241 gtacaacgag gaacacggca ttacgccgca aggcttgaac aagaaagtgg tcgatatcct
     9301 ggcgctgggg cagaacattg ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat
     9361 tgttgagccg gataatgtgc cgatggatat gttgccgaaa gcgttgcagc agaaaatcca
     9421 tgaactggaa gggctgatga tgcaacacgc gcagaatctg gagttcgaag aagcggcaca
     9481 aattcgtgac cagttgcatc agttgcgtga gttgtttatt gccgcatcgt aacaggatag
     9541 cgaagaagac tgatgacaaa cggaaaacag tctgatgcgc tacgcttatc aggcctacat
     9601 tttctccgca atatattgaa tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca
     9661 tccggcataa acaacgcgca ctttgtcttc aataggaagc cggaattttc cttcggattt
     9721 ccgttaacct aaagcctgta acgccttttc cagcgcgtta tgtaacaact ggcggtcatg
     9781 acgatacgga atatcgctgg cctccagtac ctcctggatc acaatccgct ctttcaccgc
     9841 cgagacatcc acttttggcc cgacgatgac cgcatcaatg acctttttac caacatactg
     9901 ctccataatt gccagcttgc tttccagctt caaattagcc gcaggtaaac tcaactcacg
     9961 ccccagattg ccgatataaa ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc
    10021 cttcagcagc agaattggca tcaggctggt ataaaaactg ccaggcccaa taatgatgag
    10081 atccgcttca ttgatagcgt gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa
    10141 taactcttga atcggcgtag ttaactggtc gatattgacc tcgccgtaaa cttcatgccc
    10201 ctgatcgtca atcgccatca gatcaacagg atgctctgac attggaatca aatgcgtatc
    10261 cactttcagc agattacgaa ttaaattgat ggcttccaga ggccgcacgc taaggtgatc
    10321 cagcgccttt aacatcaagt ttccgagatt atgaccggaa agttcgccat tgccgccaaa
    10381 acggtattca aacatcgcgg aggcgacgtt cggttccgtt atcagctggt tgaggcagtt
    10441 gcgcatatcg ccccaagcaa tgccgccttc tgaacggcga atacgccccg tcgagccacc
    10501 attatccgtg gtggtgacga tacccgttaa acgagaaccc aaagacgaaa gtgatgagag
    10561 aacgcgtccc agtccatgcc ctccgccgag agcaacgaca cgatcaagat cagccagcgt
    10621 acgattgcgc atatagtttc ctggagtcag attatccgcg ctacagtagc gcaaattgcg
    10681 gggaaacagc aattaacctg ccaatattga ggatacaaac tattttcttc tatctctaaa
    10741 ggacgatgca cgctatgcct ccctgatgat gtatatcaaa gtaaaaccgc cattttccct
    10801 tattctgtag cgaaatagca cgatcatgac gctatatata tgattacata gcgaaagtgt
    10861 ggatggtaaa aatctcattt acacgctagt atcggcttaa ccactaaaca ctctagcctc
    10921 tgcacctggg tcaactgata cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc
    10981 agggcgaagg aagaaatgac ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa
    11041 ctgactgatg tatttgcgcg taagttttac tacttgcgcc tgtcgattac cgatgtgtgt
    11101 aactttcgtt gcacctactg cctgccggat ggctacaaac cgagcggcgt caccaataaa
    11161 ggctttctta ccgtcgatga aattcgccgg gttacgcgcg ccttcgccag tctgggcacc
    11221 gaaaaagtgc gcctgacagg aggagagccg tctttacgcc gcgactttac cgatatcatc
    11281 gccgctgtgc gggaaaacga cgctatccgc cagattgcgg tcaccaccaa tggttaccgt
    11341 ctggaacgcg atgtggcgaa ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc
    11401 gacagtttgg acgcccgcca gtttcacgct attaccgggc aggataaatt caaccaggtc
    11461 atggcaggga ttgatgctgc atttgaggcc ggttttgaga aggtcaaagt caataccgtg
    11521 ctgatgcgtg atgttaatca tcaccagctc gacacctttc tgaactggat ccagcatcgc
    11581 cctatccagc tgcgtttcat cgaactgatg gaaacgggcg agggcagcga gctcttccgt
    11641 aagcatcaca tctctggtca ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac
    11701 caattacgtc aacgcagcga cggtcccgcg caagtctttt gccatccaga ttacgccgga
    11761 gagattggcc ttatcatgcc gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc
    11821 gtttcctcca ttggtaaact ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc
    11881 gatctgctgg aaggcgatac ccagcaacag gcgctggaag cgcgtatttc agcggcgctg
    11941 cgggagaaga aacagaccca tttcctgcat caaaacaaca ccggtattac gcaaaactta
    12001 tcgtacattg gcggctaaaa cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa
    12061 tttatcccga cccgtattgc tattcttacg gtttctaatc gtcgcggtga agaagacgat
    12121 acctccggtc actatctgcg cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa
    12181 gccattgtga aagaaaaccg ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc
    12241 gacgatgtac aagtggtatt gattacgggt ggtactggcc tgacggaagg tgatcaggct
    12301 cccgaagcgc tgctgccgtt gttcgaccgt gaggttgaag gttttggtga agtgttccgt
    12361 atgttgtcgt ttgaagagat tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc
    12421 gctaacaaaa cgctgatttt cgccatgccg ggttcgacca aagcgtgccg taccgcatgg
    12481 gaaaatatca tcgcgccgca gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat
    12541 ttgaagaaat aagtatgtcg caactgaccc atatcaacgc cgctggcgaa gcgcacatgg
    12601 tggatgtctc cgccaaagcg gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca
    12661 tgcgcagcga gacgctggcg atgattattg atggtcgcca ccacaaaggc gacgtatttg
    12721 ccactgcgcg tattgccggt attcaggcgg caaaacgcac ttgggatctg atcccgctgt
    12781 gtcacccgct gatgctcagc aaagttgaag tcaatttaca ggccgagccg gagcacaatc
    12841 gggtgcgtat agaaacctta tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt
    12901 taaccgcggc ctccgtggcg gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag
    12961 atatggtgat tggtccggta cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta
    13021 aggtggaagc ggatgattaa agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca
    13081 gatgcaaccg aagtggctgc ggattaccca accgttgaag cgttacgcca gcacctggct
    13141 gcgcagagcg atcgctgggc gctggcgctg gaagatggca aattgctggc tgccgtcaac
    13201 cagacgctgg tgagttttga ccatccgctg actgacggcg acgaagtagc tttcttcccg
    13261 ccggtaaccg gaggttaaga tggcagaaac caaaattgtt gtcggcccgc agccgttcag
    13321 cgtaggagaa gagtacccgt ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt
    13381 caccggaaag gtgcgcaacc ataacctggg cgacagcgtc aaagcattaa ccctcgaaca
    13441 ctatccgggg atgactgaaa aagcactggc agaaattgtt gatgaagcgc gtaaccgctg
    13501 gccgctgggg cgcgtcacgg tgattcaccg catcggggaa ttatggccgg gcgatgaaat
    13561 cgtttttgtc ggtgtcacca gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat
    13621 tatggattat ctcaaaaccc gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga
    13681 ccgctgggtt gaagcccggg agagcgatct gcaggcggca aaacgctggt agttttttgt
    13741 tagccggata aggcaccgct gcgtccggca atttcaccgc tcagtacatg cgtttacgct
    13801 gcgtcgatgc acagcctcat cattttgcag tatccttaag atattcctta tatcttcagg
    13861 agatcgtcat ggacagattc ccacgttctg attcaatcgt acaaccctgg gctggcttgc
    13921 aaacttatat ggcgcaagtc tatggctgga tgaccgttgg cttgttgctg accgcatttg
    13981 ttgcctggta tgcggctaat tccgcggccg tgatggagct gttgttcact aaccgtgtct
    14041 ttttaatcgg tctgatcatc gcgcaattag cattggttat tgtgttatca gcgatgattc
    14101 aaaagctgag cgcaggtgta acgacgatgc tctttatgct ttattcggcg ctgacgggtc
    14161 ttacgctttc cagtatattc attgtctata ccgctgcttc tatcgccagt actttcgtcg
    14221 ttactgccgg gatgttcggc gcaatgagcc tgtacggtta caccacgaag cgcgatttaa
    14281 gtggctttgg caatatgctg tttatggcgt taatcggcat tgtgctggca tcgctggtca
    14341 acttctggct gaaaagcgaa gcattgatgt gggcagttac ctacatcggc gtgattgtct
    14401 ttgtcggatt gacggcgtat gacactcaga aattgaaaaa tatgggtgag cagattgata
    14461 cccgcgacac gtcgaacctg cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg
    14521 acttcatcaa cctgttcctg atgttgttac ggatcttcgg caaccgccgt taattctttt
    14581 tcaccatcac tatcccggcc atgtgtcggg atttttttct tactaaaaac gacacttaac
    14641 aagtaccagg tataaaatat gccttatgag cgagcggaat cgttttccgt atccataaat
    14701 acaaaagtat tttgtatgcg tggttttaaa tttattgatg gtgaatatta atgttttttc
    14761 taaggacaac taaaagaatg gaatcatact cgcaaaacag caataaatta gattttcagc
    14821 acgaggccag gatattaaac ggtatatggc tcattaccgc tttaggtttg gtggcaaccg
    14881 caggactagc ctggggagct aagtatctcg aaattacggc aaccaaatat gattcaccac
    14941 caatgtatgt cgccataggg ttattattac tttgtatgta tggcttaagt aaggatatca
    15001 acaagataaa tgccgccatc gcgggcgtaa tatatctgtt tttactctct ttggtggcga
    15061 ttgtcgttgc aagtttagtt cctgtatatg ccattatcat cgtgttcagc actgcgggcg
    15121 cgatgttttt aatcagtatg ctggccggtt tattatttaa tgttgatcct ggttctcacc
    15181 gttttatcat tatgatgacg ttgacagggt tggccctggt aatcatcgtg aatgcggcat
    15241 taatgagtga acggcccatt tgggtaataa gttgcttaat gattgtgtta tggccaggca
    15301 ttatctcgca tggacgaaat aagctccttg aactggcagg aaaatgccat agtgaagagt
    15361 tgtggagtcc ggttcgttgc gcttttacag gtgcattaac actctattac tattttatcg
    15421 gcttctttgg gatacttgcc gcgatagcta taacgcttgt ctggcaaagg catacgcgtt
    15481 tttttcatta gtcgtaaatg cagatactaa agaacttaac taaacttcac atcgccgctt
    15541 catttttcgc ccgtagcttc ttcgcttggc tttccagtag caggtaacag atcagcgcca
    15601 gcagcagcgg gataaagtaa tacaggacgc gataagcgag cagggcggcg ataatgctcc
    15661 ctttcgaggt atgctcccca gccagtagtg cgataaacac cgcttccagc acaccgatcc
    15721 ccgccggaat atgcacgatg acgccagcaa tactactaac cagtaacacg cccagtacaa
    15781 agaaatagtt cacgctttga ccaagtaaca gccagataat cgcccccatt accatccagt
    15841 taacgctgga aatcagcatc tgagcgaggg cgaatttcca tgaaggcagc accagctttt
    15901 gccctttgat ggtcatatgg cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga
    15961 taatcatcag taagccaatg ccgagaatac gcagcgtagt ttgatcgaca taccagtaat
    16021 ccggcaactc caccacgcct gcggtaaaga taatccctgc cagtaaaata tagcccagcc
    16081 agttggtggt aatactgagc gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc
    16141 gggagtacaa acgatagcgc ataccaatgc cgccgaccca ggtactgagc gtcaggttga
    16201 aggcgtagca gataaacgac accagcatca cctggcgctt cgccagtttg tgaccgcagt
    16261 aaaagcgggc gagcaggtca tagcagccgt aaatcagata gctgacgacc accagcccga
    16321 ccgcactaag cagcgcaacg cgattgtagt cgcggatgac cttccagacc tcttcccagt
    16381 ccactttttt ggcgtagacc accagcaaca caatcaccgc gataaaaaac agccaggtga
    16441 ggatcttctt tgctaagcgc cagcgcgggt gtgatttact catcaggggt ttacccccgt
    16501 gttttcagtt tctacccgat cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg
    16561 cgtcagacgt ggtgtgtgtg ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa
    16621 gtggaacgcc agcacgcttt tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc
    16681 atccacctgc tgacaatctg cggcaataat gccgttcaga ttatcgcgca gcgtctggtt
    16741 aaaatgacga tcgtggatga tgacatttgc ttcgagattc agtgacaaac tgagcggatc
    16801 gagattactg gaccctactg tcgcccagtg atcgtccatc aatgccactt tgccgtggag
    16861 cgggcggcgg cggtactcaa aaacctgaac gccgccttta accagatagt tatacagcaa
    16921 gcgcgcaccg actctgacaa tcggcatatc cggttcgccc tgaatgatca gtttgatccg
    16981 caccccgcgc cgtgccgctt tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta
    17041 ggcgttggcg ataatcactt cccgctgcgc ctgagtgagc attttcagat aatggcgttc
    17101 aatatcatcg cgatgttctt cgttatcgcg ccagaccagc aatacctgcg cttctcccgg
    17161 ctggcggttc tcttccgctt tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtct
    17221 aggcaggttt tccagctcaa actggagaat atcttcgaca atcggccctt caaggcgtac
    17281 cgcgtaatcc tgtttagcct ctggaccgta gctggacata tgctcggcgg agtaattcag
    17341 cccgccaata aaggcgatac gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa
    17401 cacattggtg cgcataccaa aaaggcgagg gcggggatcg tagtagcgga acactacgcc
    17461 agctgccgtc agttcattga caaactcatc gctgagatcc ggcgaaccgt agccatccag
    17521 caagacttcc gctttaaccc cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt
    17581 gccgacgtta tcctcaaacc agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc
    17641 aatcgcctta aacaccgcgg gataatattg ctcgccgttt tccagcaact ggatcttatt
    17701 gccttcgcgc cagctacatt tcataaatga atctccgcac ttaaaggggc atgatcagaa
    17761 aggtgtcgcc atgtccgcag cggcaacgcg gttggcgcgc tggcgctggc atttttgacg
    17821 tagatcctgt ccagtcgtag tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg
    17881 tgggcgcggg taaaaatctc atccagtccg gcctgcactt ttaacggatg attagctttt
    17941 tgccgccagt cattgaaatc acccgccacc aataccggtt cgccgtccgg tagctcattc
    18001 acccattcgg cgagcatcgc aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt
    18061 acgcacatca catgaatcgc ttttccggtc atcggcggca caatgcggca gtagagcacg
    18121 ccgcgctttt ccgcaccatc gaccgaaaca tcgcgattct cataatgttc aatgggataa
    18181 cgcgacagta cggcgttgcc gtgatgccct tccgggtata cggcattgcg accgtaggca
    18241 aaatcgctcc acatagtgtc ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc
    18301 acatgcagcg gatgaacttc gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg
    18361 ctgacggtac gcacggcatc gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta
    18421 aagcctttgt gaatgttgat ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc
    18481 ataattttcc tgtctttgcc tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag
    18541 gtgcggtctt acggaatttt ccgtaaagtt cggtactctg agtaagtaga gataaattct
    18601 tcaggagaga agccatgaag tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct
    18661 ggcagattat gttgcattta ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga
    18721 ctctggttca cgtcggcgtg ggattatgcg cactgtattg tgtcacggta gtgatgatgc
    18781 tggtgtttca gcgccacccc gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc
    18841 tgaccacgac ctggtatttt ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac
    18901 tggaaaacaa ctttttgctg gcaattgcag ggctggcgat ccttgctggc ccggcggtag
    18961 tgtctttgct ggcgaaagat aagaagttac atcaccttac gtctaaacat cgcgtacgcc
    19021 gctgaccctg tcgtggccgt tatcaccagt agcggccaca aactattcca cacaatatcc
    19081 agactcgcat ccttcaaata aatctgcttg gtaatgtccg taaagtggcg aatagggtta
    19141 atccacgtca gattttgcag ccacactggc atgttttcca ccggcgaaac gtaaccggaa
    19201 aggagaatgg cgggcatcat aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag
    19261 cagagtgatg aaatcaacag accgaatccc accagcgata aaccgtaaat caccatcgta
    19321 aagtagaaca gcgctagcga tccggcgaag gggatttgat acgcccagat accaatcgcc
    19381 agcacaatgg tggcctggaa ggtggcgaca attaacgccg gtacggcttt gccgatgaag
    19441 atctgccagg tggtgagcgg cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg
    19501 cgggcgacgg aaagtgaagt gacgatcatt acgccgatag tggtgatcat ggcgatcagc
    19561 gacggcacca caaaccattt gtagtcgaga ttcgggttat accagttgcg taccaccagc
    19621 tcgctgttgt tgggtttcgg ttttccttcc agcagctcct gctgataatt tttgacgatc
    19681 tgttgcaggt agttggcggc aatttgcgca ctgttggagt tacgcccgtc gaggatcaac
    19741 tgcaacggcg cggtctggaa ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc
    19801 agtagtaacg ccttttgtgt gtcgatggtc gggcggatct cctgtgggct tttcagcagc
    19861 agcacatgtg taaaggcgct ggcgcgggca aaacgttgag taagttccac cgaatgctcg
    19921 ccgttatctt catcgtagat ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac
    19981 gggaacagga tcacctgaat tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg
    20041 cgcagcaacg actgcaactc tttgcggatt aacgtccata agcgatgaaa catgctcttc
    20101 tccctaatcc agccgacgtt tggttttcag ccacgtcagg ccgataaaca tcaccgccga
    20161 agcgatcaaa aacagcacgt ttaccaccag caccactgga atattcccgg cgaggaacag
    20221 gctttgcagg gtgctgacga aataacgagc gggaataatg tacgtcaccg cgcggatcac
    20281 cgcgggcata ctgtcgatct gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc
    20341 ggcgttcagg gcgacctgag cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag
    20401 ccccattccc agggtactga gtaaaaacag gctggagata aaaaacagaa tcagcagcga
    20461 accgcgatac ggcacgccga gaataaacac tgacaccagc atacacagca acatcgccag
    20521 catcccgaga aagtaataag ggatcagctt acacagcagc agttccgtgc gggtaatctc
    20581 cgtagagagc agagcctcca tggtgccgcg ttcccattct cgcgccacca ccagcgaggt
    20641 gagaatcgcg ccgatgaccg tcatgataat ggtcaccgca cccgggataa taaagtgctg
    20701 gcttatcgcc gccgggttaa accagtagcg ggtttgtaca tcaataagcg gttcaaaagt
    20761 ctgcccgttg tcctccgctc gctgcatttg ccagatctgc cagatccctt cgacataccc
    20821 ctgcacaaag ttagcggtat tcggctcact gccgtcggtg atcacctgaa tcggtgcggt
    20881 ggcgttggcg cgctccatct gttcggcgaa atccacggga ataaccacca gaccgcgaat
    20941 tttccccgac tgcattttgg cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat
    21001 gtagggcgaa ccggtcatgg tgtgggtgaa atccagcgcc gcttcgctac gctgttccag
    21061 caaaatcccg acccgcagct tgctggagtc gaggttaatg ccgtaaccaa aaataaacag
    21121 cagtagcagt gggatcacta ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg
    21181 cgtctctttg acgcacagcg cccgcacgcg atgccaggaa aggatcgggt tactcattgc
    21241 tatgctcctt atcccagtcg tggatcaact gaataaaggc ttgctccatg gtgggatcgg
    21301 gttgctcatc gttagctgac tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt
    21361 tcccacggta caccaggccg atgcggtcgc aatattccgc ttcatccata aagtgggtgg
    21421 tgaccatcac cgtgacgcct ttctctacca tgctgttgat gtgcagccaa aattcacggc
    21481 gggtgagggg gtcaacgccg gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca
    21541 tcagcgaaca ggccagcgcc agccgctgtt taaaacctaa tggcagttca tcggtggcgt
    21601 gtgaggcaat acttttcagg ccaaatgcct cgctcatacg ggagattttt tcgttctgcg
    21661 cccgaccgcg taagccatac acaccagaga aaaagcgtaa attctgttcg accgtcaggt
    21721 tgccgtagag cgaaaatttt tgcgccatat agccgagatg ctggcgcgct ttaccggaac
    21781 tctctttcag atccatcccc agcaccagcg cctggccgga agtcggcacc agcaaaccgc
    21841 acatcatctt aaaggtggtc gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct
    21901 ccccacgttt aacggcaaag ttgacgtgat cggtggcggc aaaatcacca aatttcttgg
    21961 tcagttcttt cgcttcgatc accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg
    22021 cacccagcgg cgattctgag gttccggcac cgcccagcaa atcaataaac gcatcttcaa
    22081 aacgcggcgt agtttcgttg atgttgattt ccggcatccc gtcggcatgg cgaatatcgt
    22141 ctggtgtggc ctcttttttg aggatcagac gtaccgattt cccctgaatc atgccatcgc
    22201 tgacctgcgg cagtttcaag gcgcgttgca acagtttgcg gttgccctcg tgtggactgg
    22261 tcatcagaaa gctgcgtccg gccatggttt gtgtcagggc ttttggttct ccctgataca
    22321 gcaactcgcc ttcgttcatc agtaacacgt cacggcactg ctcggcttcg tcgagatacg
    22381 aggtactcca gaggattaac atcccttcgc ccgccagctc atgcaccatc tgccacagtt
    22441 cgcgccgtga gatagggtca acgccgacgc cgggttcatc gagcagcaac actttcggtt
    22501 cgcccaccag ggtacaggcc aggccgagtt tttgtttcat cccaccggag agcttgcccg
    22561 ccaggcgtcc ggtaaacggc ccaagagacg taaactccag caggcgagca aaagtttgct
    22621 tacgtgcctc gccggtgacg ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg
    22681 tgagatcttc atacagacca aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc
    22741 cgtcgttttt gatcggatca aagccaatca ccgtggcact gccgctgtcg ggtttcagta
    22801 atcccgccaa catccgcatc agtgtggttt tacctgcacc gtccggcccc accaaccccg
    22861 tcacataacc ggcgtgaatg gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg
    22921 gaaagcgttt ttccaggccg ttcagcgtga taacggcatc attcatgtcc tgcctcgtca
    22981 ccgaattgta ccgtcactgg cattccctgg cgtaacgcat catcggcgtc ggtcaccaca
    23041 atacgcaggc gatagacgag gtcggtacgc agatccggtg tttcgacggt tttcggggta
    23101 aattcagcag tcggcgaaac gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca
    23161 tcggtataaa gcagcacttt gcgccccggc tgggcctggt caagattccg ttcatcaaca
    23221 taagcgcgca cccacaccgg acgcgttagt gaaacggtaa acaccgtgcc accttcattg
    23281 agaaccgtgc ctggctctac cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac
    23341 gttgagtcct gtaaattcag ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg
    23401 gctttcgcct gagcgatgtc ctgttcacgg ttaccggaac ggtactgacg caatttatcc
    23461 tgtgctgatt tcagcgttgc ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca
    23521 tttgccgaaa tggtgcggct tttccacagt ccttgctggc ggttatagaa gttctgcgca
    23581 tagtcatagg cggcttgcgc ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg
    23641 cgatacccgg caagcatcag atcatactgc gcctgtgcca ccgagacgcc cgctttcgcc
    23701 tgcatcaggg caatctcata cggcttgtga tccagttcgc ccagcacctg gcccgctttg
    23761 atagcatcac cttcgtccac cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga
    23821 ttcaccgtac gaatatccac gttgccatac agcgtcaggc cgttatcctg gcggctttga
    23881 taccaccagt agcctccggc aaccacggcg gcaagtacca ctaccgccag tccgatcacg
    23941 acaggttttt tcatcactac aaactccttt gcgataatcc ttgcaaaatc aggtcgatat
    24001 gacaggtcac cgtctggttg atcagttcgg tcttttcttc atcgaacgcg gtccagccgg
    24061 tacgtaacag aattgtttct ttgccaagac ggaacgccag aatctcacca atcaacgcat
    24121 gggtatgaag gatcatgcgg gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc
    24181 gtgtcaggtg gctgtgtagc ggactaatca cctgttcgtg caccaggtgg taggctgccg
    24241 tgggagagag ctgctcacgg gagataaact tgctgaggtt gacggtgtca tcctgggtga
    24301 gcagcttaat catgttcctg caggcgcgaa ggatcagttc acggatggca gcccgatcag
    24361 gctgtggttg ggcgaacaag cgttcggctt cctcggcatg cggacggaac tgctcgccaa
    24421 taaaatcggc aatccactgg gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt
    24481 aggtgatggc agcgatattc tgcccggcct gggcggctat ctcgcgggtg gtggcgttca
    24541 ttccatattc accaaactgc gccagtgcgg cagcaatcag ctgttttttc gcctgttcac
    24601 ccttgattgt catggcagga ttattcatcg cacagtccat tcttaatcaa atgattgatt
    24661 aagattatga ctccatatgg gagttgtcca gtatggctaa gaattttagc tacgccagtc
    24721 acagggataa tttatgcgct gcgtcacaaa aactgctaca ctccgctccc tcatgacatt
    24781 gtggtttttg tcattttcct tttcagtatc tccctgaaaa ctacaccggt aacggtcggg
    24841 gcggttcgga gtagttatgt ctttcgattc tttgggttta agccctgata tcctgcgcgc
    24901 cgttgccgag cagggttacc gtgaacccac ccctattcag cagcaggcga tccctgctgt
    24961 gctggaaggc cgcgacctga tggctagcgc ccagaccggc accggcaaaa cagcgggctt
    25021 tacgctgccg ctgttgcaac acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc
    25081 ggtacgtgcg ctcattctta ccccgacccg tgaactggcg gcacagattg gcgaaaacgt
    25141 ccgtgattac agcaaatacc tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat
    25201 taacccacaa atgatgaagt tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg
    25261 tttgctggac ctggaacatc aaaatgcagt gaagctggat caggttgaaa tcctcgtcct
    25321 cgatgaagct gaccgcatgc tcgacatggg ctttatccac gatatccgtc gcgtgttaac
    25381 aaaactgcca gcgaaacgcc agaacctgct gttctctgcg accttctctg acgatattaa
    25441 agccctggcg gaaaaactgt tgcacaaccc gctggaaatc gaagtggcac gccgcaatac
    25501 cgcgtctgat caggtgaccc agcacgttca ctttgtcgat aagaaacgca aacgcgaatt
    25561 gctgtcgcac atgattggta aagggaactg gcagcaggtg ctggtgttta cccgtaccaa
    25621 acacggcgct aaccatctgg ctgagcagct caataaagat ggcatccgta gtgtggcgat
    25681 ccacgggaat aaatcgcaag gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga
    25741 cattcgcgta ctggtggcga ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc
    25801 gcacgtcgtc aactatgaac tgccaaacgt accggaagat tatgtccacc gtatcgggcg
    25861 taccggtcgt gcggctgcta ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa
    25921 actgctgcgt gatatcgaaa aactgctgaa aaaagagatc ccgcgcattg cgattccggg
    25981 ctatgagccg gacccgtcaa tcaaagccga accgatccag aacggtcgcc agcaacgtgg
    26041 cggcatcagg ctgatgaaca aacgcaaaac tgcctgatgc gctacgctta tcaggcctac
    26101 gtgaactctg caatatattg aatttgcatg cttttgtagg ccggataagg cgtttacgcc
    26161 gcatccggca tttcacaaca agcacttgtc agcaatttga gaacacggga aaaaatttat
    26221 ctgttttacc gcccataagc caccgagacg ttacttctct atagccaatt gttcgcgcaa
    26281 ctccattaaa aggtagccca gtcgattctt gcccttacca ttaccaccgt ctccccagta
    26341 agcatcgttt tccgtatgct caaccagttt tgcgggcgcg gttgccagca agagcacacg
    26401 cagttctgca tgctgttcga atttagcgcg aagtgcttta cgcatcacct gttctttaac
    26461 cgactcccag tttttacgaa gtggcttgga acgattacgc cccatgcgag cggcgaccat
    26521 gggggaagaa acccgacgga tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg
    26581 aaaatagtgt tcggaggtgg gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa
    26641 gttagaaaaa tcaccgtagt catcgctggt gctgtagaaa tttatgatgg tgtcttgcat
    26701 gacgtgttgg atcctttgtg ctcgaacggg cattaaaccg cattatgttg gtggttattg
    26761 cgagccgctt tccagaaaca gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata
    26821 ttggctgttt atacagtatt tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc
    26881 aaattgccgc ctggtataag gcgcttcagg aacagatccc cgactttatt ccccgtgcgc
    26941 cgcagcggca gatgattgcg gacgtcgcca aaacgctggc cggagaagaa gggcggcatc
    27001 tggcgattga agcccccacc ggcgttggga aaacgctctc ctatttgatc cccggcatcg
    27061 ccattgcccg cgaagagcaa aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg
    27121 atcagattta cagcaaagat ttaccgctgc tgaaaaagat cattcccgat cttaaattca
    27181 ctgccgcttt cgggcgtggg cgctacgttt gtccacgtaa tctgacggcg ctcgccagca
    27241 ctgaacccac gcaacaggat ctgctggcgt ttcttgacga cgaactgacg ccaaataatc
    27301 aggaagagca aaaacgttgt gcgaagctga agggcgatct cgacacttat aaatgggatg
    27361 gtctgcgcga tcatacggat atcgctatag atgatgatct ctggcgtcgt ttgagtaccg
    27421 acaaagccag ctgcctcaac cgtaactgtt actactatcg tgaatgcccg ttttttgtta
    27481 ctcgtcggga gattcaggaa gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg
    27541 cgatggaaag cgaagccgta ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag
    27601 gtcatcacct gccagatgtg gcccgggatg cgctggagat gagcgctgaa atcaccgtgc
    27661 cctggtatcg actacagctg gacttgttca cgaaactggt cgctacctgc atggagcagt
    27721 ttcgcccgaa gaccatccca ccgctggcga tccctgaacg tttgaatgcg cattgtgaag
    27781 agttgtatga gcttatcgcc tcgttaaaca acattctcaa tctctacatg cctgccgggc
    27841 aggaggcaga gcaccgtttt gcgatgggcg aactgcctga tgaattgctg gagatctgcc
    27901 agcggctggc aaaactcacc gagatgctgc gtggtctggc ggagttattt cttaacgatt
    27961 taagtgagaa aaccggcagc cacgatattg tgcgtctgca tcggttgatt ttacagatga
    28021 accgcgcgct ggggatgttt gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc
    28081 aatcttccgg tgcgccggtg accaaatggg cgacgcggga agagcgcgaa gggcagctac
    28141 acctctggtt tcactgcgtg ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc
    28201 gcagtattcc gcacattatt gtcacctccg caaccttgcg ttcgctgaat agtttttcgc
    28261 gtttgcagga gatgagtggt ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt
    28321 ccccctttaa ccactgcgaa cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt
    28381 ccatcgacaa cgaagagcaa cacattgccg aaatggcggc ctttttccgt gaacaggtgg
    28441 agagcaaaaa atatctcggt atgttggtac tgtttgccag cggacgggcg atgcagcgct
    28501 ttctcgacta tgtgacggat ttacgcctga tgttgctggt gcagggcgat cagccgcgtt
    28561 atcgcttagt tgaactgcac cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg
    28621 gcttacagtc atttgccgaa gggcttgatt tgaaaggtga tctgctcagc caggtgcata
    28681 tccacaaaat cgcttttccg cccatcgaca gcccggtggt gatcaccgaa ggggaatggc
    28741 tgaaaagcct caaccgctat ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc
    28801 tgattcagca ggttgggcga ctgattcgaa gccacggttg ctggggcgaa gtggttatct
    28861 acgacaaacg cttgctgaca aaaaattatg gcaagcgact actggatgca ttaccggtat
    28921 ttccgataga gcaaccggaa gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat
    28981 ccccacgccg tcggcggcgt taatgatgtg agtcaggtaa ggagtcgtaa atggactatc
    29041 gcaaaatcat taaagagatc gggcgcggga aaaaccacgc gcgcgattta gaccgggata
    29101 ctgcgcgcgg tctgtatgct catatgctca atggtgaagt ccctgacctt gagttgggcg
    29161 gcgtattgat tgcgctgcgt atcaaagggg aaggggaagc agagatgctc ggcttttacg
    29221 aagccatgca aaatcacacc atcaagctga cgccgcctgc aggcaagccg atgccgattg
    29281 tcatccccag ttacaacggc gcgcgtaaac aggctaacct gacgccatta ttggcgattc
    29341 tgctgcataa actcggtttt cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg
    29401 tgctgacaga aaccattttc gaactgatgg gtattacacc aacgttgcac ggcgggcagg
    29461 cgcaggcgaa gctcgacgaa catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc
    29521 cgctggaaaa acaactggcg atgcgctggc ggatgggcgt gcgtaacagt gcacataccc
    29581 tggcgaaact ggcgacgcca tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc
    29641 atccggaata cattggacgc gtcgcgaagt tctttagcga tatcggcggg tgggcgttac
    29701 taatgcatgg tactgaaggc gaagtgtatg ctaatccgca gcgctgcccg cagatcaatc
    29761 tcattgaccg tgaagggatg cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt
    29821 tactgccaca agcaaaagat ccggaaacca cggcgcagtg gactgagcgt tgcctggctg
    29881 gcagcgaagc gattcccgaa tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg
    29941 gtgaagcggc aactatcagc gatggcctgg cgcgcgttaa tcaggcattt taatttttat
    30001 ctcagccgga tgagccacgt catccggctt ttaccccacc gcaatatgaa attcctgcat
    30061 ctttattgac cttcccgcgc ccggcgtgca gcataaaaaa acaacaaaca cataacataa
    30121 acaggagtta accatggaaa gtggtcatcg ctttgatgct cagacgctgc acagttttat
    30181 tcaggctgta tttcgtcaga tgggtagcga ggaacaagaa gcgaaattag ttgccgatca
    30241 tttaatcgcg gcaaacctgg cagggcatga ttctcatggt attggcatga tcccaagcta
    30301 tgtgcgttcc tggagtcagg ggcatctgca aattaaccat catgccaaaa tcgttaaaga
    30361 ggcgggggcg gcagtcacgc tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga
    30421 agcgatggcg ctggggattg agaaagcgca tcagcacggt attgctgccg tggcgctaca
    30481 taactcgcat catatcggcc gtatcggtta ctgggcggag cagtgttcag cggcggggtt
    30541 tgtctctatc cactttgtta gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg
    30601 cgacagccgc tttggcacca atcccttctg tgtggttttc cctcgtaaag ataatttccc
    30661 gctgttgctt gattacgcca ccagcgccat tgcatttggc aaaacccgcg tcgcctggca
    30721 taaaggcgtc cccgtgccgc caggttgcct gattgacgtt aacggcgtgc cgacgaccaa
    30781 tccggcggta atgcaggagt cgccgttggg gtcgctgttg acctttgccg aacataaagg
    30841 ctacgccctt gcagcgatgt gtgaaattct tggcggggcg ctttctggcg gtaaaacgac
    30901 gcatcaggaa acgttgcaaa ccagccccga tgccattctt aactgcatga ccactatcat
    30961 catcaacccg gaactgttcg gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga
    31021 gtgggtgaaa gcctcgccgc atgatgacga taagccgatt ttgctaccgg gcgagtggga
    31081 agtgaacacg cgtcgcgaac ggcaggagca ggggattcca ttggatgcgg gaagctggca
    31141 ggccatttgt gatgcggcgc ggcagattgg tatgtcggaa gagacgttgc aggctttctg
    31201 tcagcagtta gccagctaaa aaaaagcccg tccagtggcg gacgggcaaa caagggtaac
    31261 ataggatcaa tgagggttag agcatatgcg tctgtcggca aacagacagg gaaatacttg
    31321 tgctggacgt agcgtaaacg cctgatccgt ccaacggttc ggattttgta ggcctgataa
    31381 gacgcggtag cgtcgcatca ggcatctgtt gccggatgcg gcataaacgc cttatccgtc
    31441 ctacgaatta cttataaatt accgcagtac cgcttaattt attgttgttg gtggcggaag
    31501 tgatgctgta tccactggca ccggctgccg ccgctttctc agccagtttc gcttccaggg
    31561 catcgagggt ggatgcgcca tcggcagaaa ccacgccgat tttattcatg ttctgtgcct
    31621 gggatgccgt taccggttcc gccgcgaaca cgccaaatga cagagttgaa agagccatag
    31681 cagcaacaac agtattgata gttttcataa ttaatctctc gcaggtgatt tttgtataag
    31741 tgacgttgtt tcgtcgatgt gatgagtatc acgttttttt acgagagata aaatcgaaga
    31801 gaattgaccg ccttgttcaa ataaattgat tgatatctaa catactgata aaccatttat
    31861 ttgttgtact tatggacttg ttttatgccg cgtggcagtc acagcagcgt taaaggcacg
    31921 gaatgccacg caaaatacaa attatactcg ctatgcatct cgccagttgt agttaacgta
    31981 actgtctgtc tttcgaacct ctgcgattat atcctgtata cgctggtttt tgtaaatctt
    32041 tctcctgctg acaatgaata cataagcgca cgctaggaat ggcttcccga cgggcttgcg
    32101 ggatgggggc accgcactct tcacattcat acaggctttc gccacgcgga atttcacccc
    32161 gagcgcgggc aatcgcatct tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt
    32221 tagcccaagc ggatgccata tcgtcctccc catatcaatg cttgtacagt taagtgtagc
    32281 taatataggg acgaacctgg gcagttcaag cgtcagatct ccgaccattc ccgcagcaga
    32341 ttatgataaa gattgagcag cgacaggatc tcttcatttt caccatagcg gcttttcagc
    32401 gactgaatat ttttgtccag ttcaaacagc atggcgcgct ttttatcatc gcggatcatc
    32461 gactggatcc acataaatga tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc
    32521 aggctgctgg agggatacaa caccagatca cctgccggga gttttacccg atgttgtcca
    32581 aaggtgtcat taacgaccag ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc
    32641 gtggcagaaa ggtcagtgcg catccagccg ttttgtggat ggctgcgtac tgcgccatcc
    32701 acatggaaac cataggtttc attgttctga tagcgattaa acagcggcgt ggaaagggta
    32761 cgcggcaagg cagcggcaaa gaacaatgca tgttggttaa ccgcgttcag cacctcattt
    32821 tgcagtgcgg cgtataacgc gctgcgggtg tctacttgct gattgttctt aacttgtgca
    32881 ccttgtgctc cggtggtgac gcgtccatcc acccattcgg cttgttccag ttgttcgcga
    32941 aaatgggcga cgtcctgtgg cgataacacg ccgggaatgt ggtacatcat acaagttctc
    33001 caaaaagtgg ggcctgcgcc ccacatctga atcagaaatg catattggct gtgagcaaga
    33061 aggttcttgg ctcgcccggg tgataacggt agccgctctt gttgattgag gcgacgtaat
    33121 cggtatcaaa caggttgtaa acgtttagct ggaagtcgag attgcgatta acccgatacc
    33181 ccagtttggc atcggcgacc cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt
    33241 ctgaaccttt atgtatactg ccgatatagc gtgcgcccgc gccaacagag atatcatcgg
    33301 ttgcctgata ttggctccat aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg
    33361 aaccatcctg ggcaacatct ttgccgtttt tgatggttgc tttttgctgg gtatagccgc
    33421 caatcacctg ccacgcggga gtgatattcc ctgccacgga tatctcatag ccttcgacgc
    33481 gtttcttacc gtattgcgag taagtcccgt catcattttg ctcaacttca ttttcgatat
    33541 cagtacggaa cagcgcggcg gtgagcaaca ggcgtttatc cagaacctgc catttggtgc
    33601 caatctcgct ggtgttggct ttttgcggtt taaaatcggt gcggttggca ctgttaccac
    33661 tgccagactg cgcaagggcg aagttgttgc cgcccggagg ctgctgggaa acggcatagt
    33721 taatatagac attgccgttt tccgtcaggt ggtacagcgc cccggctttc cagttcacca
    33781 gattgcccga cttggcggtg tcgacggtgg tgactggaga accttttgcc acaccagctg
    33841 ggcaggtgat ggcaccgcgt ccgctgccgc cgcaggcggt ggcactgtca tattcagtat
    33901 gataattatc cagacggatc ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat
    33961 cgaaggcgta aattgcgaag gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca
    34021 ggccgccggg atgaattctg ctgtcaggat gataaatatt taccgccggt aacgtcaccg
    34081 gattaacgcc atagttagtc tgcgtttcac gggtaaattc cacgccggta ctgacatcat
    34141 gaccgataga accggtatag aacgtcgagg tcaggttggt ctggttggtg agaattttat
    34201 tactcacatc tttggtattc gccgtgcgtg actaggtcca gctattcaca tcgctggtgg
    34261 gctgagtaat attcgacgcc ccgcccataa tcgccgtcat caggtaatcc tgctttacgc
    34321 gcgaccaacg ggtagtattg cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca
    34381 tggtggcggt gtcggtggtc gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag
    34441 tatcaacttt tccggaatga ttcagggccg ccgctcccgc agatggggca gaatagcccg
    34501 gcaaaccgat ggtcggaatg ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat
    34561 taagatacaa acgatttgct gtaccaaggc caaaagcgac agaaggggcg acgccgtaac
    34621 gctcattttt gactttgtcg cgtccggcat cgtgggtttt ttcgcccatc acattcaggc
    34681 gcaccgcggt agtatcacca atgacctgat tgacgtccag cgtgccgcgg cggaaccagg
    34741 cgctgccaat actggcggag gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca
    34801 tattgatcga gcctgtcggt gcgctgcgcc cgtagtcggt gccggacggc cctttaatca
    34861 cttcgacctg ctcggtattg aaggtgtcgc gcgagacgct gccgatatcg cgaataccat
    34921 caatataaat actgttagag gtatcggcac cacgcatata aatggcgtcg ccagtggtgg
    34981 agttaccgtt ctcacccgca aaaaacgcac ccacgc
//
LOCUS       DAETQL010000038        34903 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.038, whole genome shotgun sequence.
ACCESSION   DAETQL010000038 DAETQL010000000
VERSION     DAETQL010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.038"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            858..3143
                     /gene="nrdA"
                     /locus_tag="K0W65_002010"
     CDS             858..3143
                     /gene="nrdA"
                     /locus_tag="K0W65_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBI3095280.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3377..4507
                     /gene="nrdB"
                     /locus_tag="K0W65_002011"
     CDS             3377..4507
                     /gene="nrdB"
                     /locus_tag="K0W65_002011"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBI3095281.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4507..4761
                     /gene="yfaE"
                     /locus_tag="K0W65_002012"
     CDS             4507..4761
                     /gene="yfaE"
                     /locus_tag="K0W65_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBI3095282.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="K0W65_002013"
     CDS             complement(4815..5465)
                     /gene="inaA"
                     /locus_tag="K0W65_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBI3095283.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5644..5886
                     /locus_tag="K0W65_002014"
                     /pseudo
     CDS             5644..5886
                     /locus_tag="K0W65_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="K0W65_002015"
     CDS             complement(5928..7004)
                     /gene="glpQ"
                     /locus_tag="K0W65_002015"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBI3095284.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="K0W65_002016"
     CDS             complement(7009..8367)
                     /gene="glpT"
                     /locus_tag="K0W65_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBI3095285.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8640..10268
                     /gene="glpA"
                     /locus_tag="K0W65_002017"
     CDS             8640..10268
                     /gene="glpA"
                     /locus_tag="K0W65_002017"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBI3095286.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10258..11517
                     /gene="glpB"
                     /locus_tag="K0W65_002018"
     CDS             10258..11517
                     /gene="glpB"
                     /locus_tag="K0W65_002018"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBI3095287.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11514..12704
                     /gene="glpC"
                     /locus_tag="K0W65_002019"
     CDS             11514..12704
                     /gene="glpC"
                     /locus_tag="K0W65_002019"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBI3095288.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12897..13847
                     /locus_tag="K0W65_002020"
     CDS             12897..13847
                     /locus_tag="K0W65_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBI3095289.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13888..14358)
                     /locus_tag="K0W65_002021"
     CDS             complement(13888..14358)
                     /locus_tag="K0W65_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBI3095290.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14413..15615)
                     /locus_tag="K0W65_002022"
     CDS             complement(14413..15615)
                     /locus_tag="K0W65_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBI3095291.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15702..16244)
                     /locus_tag="K0W65_002023"
     CDS             complement(15702..16244)
                     /locus_tag="K0W65_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095292.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16523..16948
                     /gene="nudI"
                     /locus_tag="K0W65_002024"
     CDS             16523..16948
                     /gene="nudI"
                     /locus_tag="K0W65_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBI3095293.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16987..17589)
                     /locus_tag="K0W65_002025"
     CDS             complement(16987..17589)
                     /locus_tag="K0W65_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBI3095294.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQNKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17897..19036
                     /gene="arnB"
                     /locus_tag="K0W65_002026"
     CDS             17897..19036
                     /gene="arnB"
                     /locus_tag="K0W65_002026"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBI3095295.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19040..20008
                     /gene="arnC"
                     /locus_tag="K0W65_002027"
     CDS             19040..20008
                     /gene="arnC"
                     /locus_tag="K0W65_002027"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBI3095296.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20008..21989
                     /gene="arnA"
                     /locus_tag="K0W65_002028"
                     /pseudo
     CDS             20008..21989
                     /gene="arnA"
                     /locus_tag="K0W65_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21986..22876
                     /locus_tag="K0W65_002029"
     CDS             21986..22876
                     /locus_tag="K0W65_002029"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBI3095297.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22876..24528
                     /gene="arnT"
                     /locus_tag="K0W65_002030"
     CDS             22876..24528
                     /gene="arnT"
                     /locus_tag="K0W65_002030"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HBI3095298.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24525..24860
                     /gene="arnE"
                     /locus_tag="K0W65_002031"
     CDS             24525..24860
                     /gene="arnE"
                     /locus_tag="K0W65_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBI3095299.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24860..25246
                     /gene="arnF"
                     /locus_tag="K0W65_002032"
     CDS             24860..25246
                     /gene="arnF"
                     /locus_tag="K0W65_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBI3095300.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="K0W65_002033"
     CDS             complement(25240..25512)
                     /gene="pmrD"
                     /locus_tag="K0W65_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBI3095301.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25617..26972)
                     /gene="menE"
                     /locus_tag="K0W65_002034"
     CDS             complement(25617..26972)
                     /gene="menE"
                     /locus_tag="K0W65_002034"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBI3095302.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26969..27931)
                     /gene="menC"
                     /locus_tag="K0W65_002035"
     CDS             complement(26969..27931)
                     /gene="menC"
                     /locus_tag="K0W65_002035"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBI3095303.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27931..28788)
                     /gene="menB"
                     /locus_tag="K0W65_002036"
     CDS             complement(27931..28788)
                     /gene="menB"
                     /locus_tag="K0W65_002036"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBI3095304.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28803..29561)
                     /gene="menH"
                     /locus_tag="K0W65_002037"
     CDS             complement(28803..29561)
                     /gene="menH"
                     /locus_tag="K0W65_002037"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBI3095305.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29558..31228)
                     /gene="menD"
                     /locus_tag="K0W65_002038"
     CDS             complement(29558..31228)
                     /gene="menD"
                     /locus_tag="K0W65_002038"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBI3095306.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31317..32612)
                     /gene="menF"
                     /locus_tag="K0W65_002039"
     CDS             complement(31317..32612)
                     /gene="menF"
                     /locus_tag="K0W65_002039"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBI3095307.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="K0W65_002040"
     CDS             complement(32691..32996)
                     /gene="elaB"
                     /locus_tag="K0W65_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBI3095308.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33051..33512)
                     /locus_tag="K0W65_002041"
     CDS             complement(33051..33512)
                     /locus_tag="K0W65_002041"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI3095309.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33577..34494
                     /gene="rbn"
                     /locus_tag="K0W65_002042"
     CDS             33577..34494
                     /gene="rbn"
                     /locus_tag="K0W65_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBI3095310.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34685..34873
                     /locus_tag="K0W65_002043"
                     /pseudo
     CDS             34685..34873
                     /locus_tag="K0W65_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8536 a         9339 c         8648 g         8380 t
ORIGIN      
        1 cgtaatgcct gaaaggcttt gcctgctggc gtgacaactc gctttgatat catatccctg
       61 gcataaatca atggccgccg cgacaccgtt agcagagaaa agagatgcaa tcattgacgg
      121 gagtagagat aaatactcct tgcgtagaaa gataatccgc atattaatct gaccatcatt
      181 ttatataaga cggcatattt aaacgcatta tacaatatgg gttatctttc ttttgtaaat
      241 tgtccaaccc gatgcaatgg ctattttgtt attatttgat gaataatatc agtgcgtcat
      301 aattcaagtt aataacctta agggatacca gttatattta aactaaatta aagtaatgaa
      361 taattttctt ataatataag gtaaattaac aaaatggctt cgcatttaac aataaccgaa
      421 tagaaaacaa ccatttcgac atcaacaatc gcttacattc gcttatatat tgaccacaac
      481 tgatacatca gattatgtga tgactcgtgc ttagatcaat ttttgcaatc attagcaaaa
      541 agattaataa accatctata tcaatttatc caacctattc tgccgttcaa gaaatcgccg
      601 aacagttatt tttaacaaat ttttctcttc ccattgactt tcccggacac cttgtctgac
      661 ctaaggtgcg cgaaagccac tttttccttc ctgagttatc cacaaagtta tgcacttgca
      721 agagggtcat tttcacacta tcttgcagtg aatcccaaac atacccccta tatatagtgt
      781 tctaagcagc ttcccttact acaggtagtc tgcatgaaac tattgcggaa agaattccaa
      841 aaacaggtac gacatacatg aatcagaatc tgctggtgac aaagcgcgac ggtagcacag
      901 agcgcatcaa tctcgacaaa atccatcgcg ttctggattg ggcggcagaa ggactgcata
      961 acgtttcgat ttcccaggtc gagctgcgct cccacattca gttttatgac ggtatcaaga
     1021 cctctgacat ccacgaaact attatcaagg ctgccgcaga cctgatctcc cgtgatgcgc
     1081 cggattatca gtatctcgcc gcgcgcctgg cgatcttcca cctgcgtaaa aaagcctacg
     1141 gccagtttga gccgcctgcg ctgtacgacc acgtggtgaa aatggtcgag atgggcaaat
     1201 acgataatca tctgctggaa gactacacgg aagaagagtt caagcagatg gacacgttta
     1261 tcgatcacga ccgtgatatg accttctctt atgctgccgt taagcagctg gaaggcaaat
     1321 atctagtaca gaaccgcgtg accggcgaaa tctacgaaag cgcccagttc ctttatattc
     1381 ttgttgccgc gtgcctgttc tcgaactacc cgcgtgaaac gcgcctgcaa tacgtgaagc
     1441 gtttttatga cgcggtttcc acatttaaaa tttcgctgcc gacgccaatc atgtccggcg
     1501 tgcgtacccc gactcgtcag ttcagctcct gcgtactgat cgagtgcggt gacagcctgg
     1561 attccatcaa cgccacctcc agcgcgattg tgaaatatgt ttcccagcgt gccgggatcg
     1621 gcatcaacgc cggacgtatt cgtgcgctgg gcagcccgat tcgcggtggc gaagcgttcc
     1681 ataccggctg tatcccgttc tacaaacatt tccagacagc ggtgaaatcc tgctctcagg
     1741 gcggtgtgcg cggcggtgcg gcaacgctgt tctacccgat gtggcatctg gaagtggaaa
     1801 gcctgctggt gttgaaaaac aaccgtgggg ttgaaggcaa ccgcgtgcgt catatggact
     1861 acggggtaca aatcaacaaa ctgatgtata cccgcctgct gaaaggtgaa gatatcaccc
     1921 tgttcagccc gtccgacgta ccggggctgt acgacgcgtt cttcgccgat caggaagagt
     1981 ttgaacgcct gtataccaaa tatgagaaag acgacagcat ccgcaagcag cgtgtgaaag
     2041 ccgttgagct gttctcgctg atgatgcagg aacgtgcgtc taccggtcgt atctatattc
     2101 agaacgttga ccactgtaat acccatagtc cgtttgatcc gaccatcgcg ccagttcgcc
     2161 agtctaacct gtgcctagaa attgccctgc cgaccaaacc gctgaacgac gtcaacgacg
     2221 agaacggtga aatcgcgctg tgtacgctgt ctgctttcaa cctgggcgca attaatagcc
     2281 tggatgaact ggaagagctg gcgattctgg cggttcgtgc acttgacgcg ctactggatt
     2341 atcaggatta cccgatcccg gccgccaaac gtggagcgat ggggcgtcgt acgctgggta
     2401 ttggtgtgat taacttcgct tactatctgg cgaagcacgg taaacgctac tccgacggca
     2461 gcgccaacaa cctgacgcat aaaaccttcg aagcgattca gtattacctg ctgaaagcgt
     2521 ctaatgagct ggctaaagag caaggcgcgt gcccgtggtt taacgaaacc acttacgcga
     2581 aagggatcct gccgatcgat acctataaga aagatctgga tgccatcgct aatgagccgc
     2641 tgcattacga ctgggaagca ttgcgtgaat caatcaaaac gcacggcctg cgtaactcca
     2701 cgctttctgc cctgatgccg tccgagacct cttcgcagat ctctaacgcc actaacggta
     2761 ttgaaccgcc gcgcggttac gtcagcatca aagcgtcgaa agacggtatt ttgcgccagg
     2821 tggtgccgga ctacgagcac ctgcacgacg cctatgagct gctgtgggaa atgccgggta
     2881 acgatggcta cctgcaactg gtaggtatca tgcagaaatt tatcgatcag tcgatctctg
     2941 ctaacaccaa ctacgatccg tcacgtttcc cgtcaggaaa agtgccgatg cagcagttgc
     3001 tgaaagacct gctcaccgcc tacaaattcg gggtcaaaac gctgtattat cagaacaccc
     3061 gtgacggcgc tgaagacgcg caggacgatc tggtaccgtc aatccaggac gatggctgcg
     3121 aaagcggcgc atgtaagatc tgatattgag atgccggatg cggcgtaaac gccttatccg
     3181 gcctacggct cggtttgtag gcctgataag acgcgccagc gtcgcatcag gctccgggtg
     3241 ccggatgcag cgtgaacgcc ttatccggcc tacggctcgg atttgtaggc ctgataagac
     3301 gcgccagcgt cgcatcaggc acaggatgcg gcgtaaaatg ccttatccgg cattaaactc
     3361 ccaacaggac acactcatgg catataccac cttttcacag acgaaaaatg atcagctcaa
     3421 agaaccgatg ttctttggtc agccggtcaa cgtggctcgc tacgatcagc aaaaatatga
     3481 catcttcgaa aagctgatcg aaaagcagct ctctttcttc tggcgtccgg aagaagttga
     3541 cgtctcccgc gaccgtatag attaccaggc gctgccggag cacgaaaaac acatctttat
     3601 cagcaacctg aaatatcaga cgctgctgga ttccattcag ggtcgtagcc cgaacgtggc
     3661 gctattgccg cttatttcta ttccggaact ggaaacctgg gtcgaaacct gggcgttctc
     3721 agaaacgatt cattcccgtt cctatactca tatcattcgt aatatcgtta acgatccgtc
     3781 tgttgtgttt gacgatatcg tcaccaacga gcagatccag aaacgtgcgg aagggatctc
     3841 cagctattac gatgaactga tcgaaatgac cagctactgg caccttctgg gcgaaggcac
     3901 ccacaccgtt aacggtaaaa ccgtaaccgt tagcctgcgc gagctgaaga aaaaactgta
     3961 tctctgcctg atgagcgtta acgcgctgga agcgatccgc ttctacgtca gctttgcttg
     4021 ttcctttgcc tttgcagaac gcgaactgat ggaaggtaac gccaagatta ttcgcctgat
     4081 tgcccgcgat gaagccctgc acctgaccgg tactcagcat atgctgaatc tgctgcgcag
     4141 cggcgcggat gatccggaga tggcggaaat tgccgaagaa tgtaagcagg agtgctatga
     4201 cctgtttgtc caggcggcac agcaagagaa agactgggcg gattatctgt tccgtgacgg
     4261 ttcgatgatt ggtctgaata aagatattct ctgccagtac gttgaataca tcaccaatat
     4321 ccgcatgcag gcagtcggtt tggatctgcc gttccagacg cgctccaacc cgatcccgtg
     4381 gatcaacacc tggctggtgt ctgataacgt gcaggttgct ccgcaggaag tggaagtcag
     4441 ttcttatctg gtcgggcaga ttgacgcgga agtggacacc gacgatttga gtaacttcca
     4501 gctctgatgg cccgcgttac cctgcgcatc actggcacac aactgctgtg ccaggatgaa
     4561 cacccttccc ttctggcggc gctggaatcc cacaatgtgg cggttgagta ccagtgccgc
     4621 gaaggttact gcggctcctg tcgcacccga ctggtcgcag gtcaggtgga ctggattgcc
     4681 gaaccgttag cctttattca gccgggggaa attttgccct gttgttgccg ggcaaaaggc
     4741 gatattgaaa tcgagttgtg atcgggtact agcgccagat acgacgctta cgcgtattat
     4801 ctggcctaca acgattacat ggcgtagtaa tacgctttca cctgctccca gtccgcttta
     4861 gggatcggct ccagatattt ttccagctgg cggaagtcat gattaatcgc tttatcgcga
     4921 cgcaagcggc gacgactttt ctccagatca agaaaaccag cttctgcctt accttctgtt
     4981 ttcacataga tatggcgaac atagcaacag ccatgctgac gattaacgct atgcattttc
     5041 ttaaacgcca gtgccactgc tttcaacatg gcttgccgta cttcgtcaga ataaggcgat
     5101 actgcatgcc gggcatacca gtcagcaatg ctgatgaacc ccgccatatc ttcagtcacc
     5161 agcaacgcgc gccattcacc ctcaattttc accgcttcac caaaaacgat cttcggcacg
     5221 atgacaccgg cccgttccag ttctttaatt accgcaacct cacggacaat cgttggtcgg
     5281 ccgaacggat aacgtactga atgaaacaga tgatgcgtca tgcgctttac atacagcttt
     5341 ttgccgttgc gctcgacgca ttgcaccccg ctcataccat tacggcgata gttaggctct
     5401 tcaacccagt cgccctctgt tgcccaccag tgattaaatt cgtcgtactt tgctgaaact
     5461 gccataccct atcgcctgtc atttttatta acgacaatga ctataggtgg ttacctgagg
     5521 aaaatcttaa tgaaacgtgt cgtattaatg aatttctttt gccgctacag gtgtgacacc
     5581 gcttttgccc ttcatttgca gcgcatagat tccaccagcc actaccacta atagtgcgcc
     5641 cagcagataa aatgtgaatt taatcagtcg gatacagcga tgaattttat tcgacaggga
     5701 ttaggcatcg ccttacaacc agagttaacg ctgaaaagca ttgcaggtga attgtgttcc
     5761 gttcctcacg aaccaacctt ctatcgacag atttcgttgc tgactaaaga aaagccggta
     5821 gaaggcagtc cactgttttt actacaaatg tgcatggaac aattagtggc gattggaaaa
     5881 atttgatatg agacaggatg gcgcatgaac gccatcctgc agtaacatta ctctttatta
     5941 agaaatttta ctgccttatc agggaaatca gtaaacagcc catttacacc cgctttgtta
     6001 tacagagcat catataactg attcacatca ggagtgtatt caggcagttt atctgaccgc
     6061 acggtataag gatgcactac cagtttattc tgctgggcat cttgcaccat gccagtgagt
     6121 ttgatattac ccggctgcga tgtctcctca atcaacatat ggtaatccgg accaatacca
     6181 tctgcatatt ccgccacctg tttcatggct cccggcttaa acatccagtc gtagttgtaa
     6241 ttaacccagc ttccatccgg ctgtttctgc tgcgtttcat tccagtcggt ataggcaatc
     6301 agctgtacca gattgagctc catgcccatt ttgggttcca gctcattctt aatacgcttc
     6361 agctcatcag catcaaaaca ttgcaaataa actttatcgt ctttaccggt gtaaccatat
     6421 ttcttcagca cttccagcgt ttttgccgca atatccttcc cttcctgatg atggaaccac
     6481 ggcgctttga tttctggata gataccgata tttttcccgg tagagtgatt taacccctga
     6541 acaaattcaa tctcttcttc aaaggtgtgc acccggaagt cggacttacc cattgggaaa
     6601 cgccccggat aagtctgtac ttttttaccg ttttcaatat cgaaaccttc ggtaaatttc
     6661 agcgacttaa tttcatccag cgtgaaatct atcgcgtagt aacgaccgtc tttgcgcgcc
     6721 cgatccggga aacgatcggc aacatcagta acacgatcga gataatggtc atgcagaaca
     6781 accagatggt cgtctttggt catcaccaaa tcctgttcca gataatccgc tccctgcgca
     6841 tacgccatcg cttttgctgg cagcgtatgc tccggcaaat atccactggc accgcgatgg
     6901 gcgatgacta ttttttcgtt gctgtccgcc gccattgcac tgcttcccat gactatagtg
     6961 ctcatcatga tcgccatgct aaggtttttc agcttcaatt tcatgccatt agcctccgtt
     7021 gcgtttttgc agtaattgtt catggcgacg tttttcgcca atcatcacaa caatcaacaa
     7081 gataaccgcc agaatgctgc cgccaatcat taccataaag ccgccatccc agccgaagaa
     7141 gtccacggtg tagccaacaa tcgcgctcgc cgccaccgaa ccgcccaggt aaccaaacag
     7201 cccggtaaag cccgctgccg tacctgccgc ttttttcggt gccagttcca gcgcatgcag
     7261 accgatcagc atcacaggac cgtagatcag gaagccgata acaatcatac aaatcatatc
     7321 gacggttggg ttacctgccg ggttcatcca gtaaacgata gtcgcgatgg tcaccagtgt
     7381 cataaagaag acgccggttg ccccacggtt accacggaag actttgtccg acatccagcc
     7441 gcacagcagg gtgcccggaa tacccgcata ctcatagaag aagtaggccc aggaggattt
     7501 atccagcgcg aaatgcttaa cctctttcag ataagtcggt gaccagtcga ggatgccgta
     7561 acgcagcaga taaacgaaca cgttggcgat ggcgatgtac cacagcagtt tgttcggcag
     7621 cacgtactgc atgaagattt gcttcgcggt cagctcctgt tccgcttttt cgttatagtc
     7681 gtccggataa tcatttttgt actcttcgat cggcggcaag ccacaggatt gcggggtatc
     7741 gcgcatcatc gcaaaggcga ataatgccac cagaatggcg cagaaagcag gcatatagag
     7801 cgccgcatgc cagtcattga accaggccat ccccagcagg aacagcagcg gcggaatacc
     7861 accaccgacg ttgtgcgcac agttccacac tgacacaatg ccgccacgtt ctttctgcga
     7921 ccaccagtgc accatagtac gaccacacgg cggccacccc atcccctgga accaaccgca
     7981 gaggaacaac agtacaaaca tcaccgcaat gctcgacgtc gcccatggca caaagcccat
     8041 aaacaacatc actgccgccg ccagaatcaa acctgcgggc aggaaaacgc gcggattcga
     8101 gcgatccgat accgaaccca tgatgaattt cgaaaatcca taagcaatcg agatccccga
     8161 aagggcaaaa cctaaatcac cgcgtgagaa tccctgctca accagataag gcatagcaag
     8221 cgcaaagttc ttacgaacca aatagtaagc cgcatagcca aagaatatcc ccaggaaaat
     8281 ttgccagcgc aatcgacgat aagtcggatc gatctccgcg gcaggtaagc gcgctttgtg
     8341 tggcgctggt ttaaaaatac tcaacattga tagcctccgt ggcccgtggt cttatttatg
     8401 attaacagcc tgattcagtg agagaacctg ccgtttcttg agttgccgcg atgttaagaa
     8461 aacattcata aattaaatgt gaattgccgc acacattatt aaataagatt tacaaaatgt
     8521 tcaaaatgac gcatgaaatc acgttttact ttcgaatcat gagcgaatat gcgcgaaatc
     8581 aaacaattca tgtttttact atggctaaat ggtaaaaaac gaacttcaga gggataacaa
     8641 tgaaaactcg cgactcgcaa tcaagtgacg tgattatcat tggcggcggc gcaacgggag
     8701 ccgggattgc ccgcgactgt gccctgcgcg ggctgcgcgt gattttggtt gagcgccacg
     8761 acatcgcaac cggtgccacc gggcgtaacc acggcctgct gcacagcggt gcgcgctatg
     8821 cagtaaccga tgcggaatcg gcccgcgaat gcattagtga aaaccagatc ctgaaacgca
     8881 ttgcacgtca ctgcgttgaa ccaaccaacg gcctgtttat caccctgccg gaagatgacc
     8941 tctcattcca ggccactttt attcgcgcct gcgaagaagc agggatcagc gcagaagcta
     9001 tagacccgca gcaagcgcgc attatcgaac ctgccgttaa cccggcactg attggcgcgg
     9061 tgaaagttcc ggatggcacc gttgatccat ttcgtctgac cgcagcaaac atgctggatg
     9121 ccaaagaaca cggtgccgtt atccttaccg ctcatgaagt cacggggctg attcgtgaag
     9181 gcgcgacggt gtgcggtgtt cgtgtacgta accatctcac cggcgaaact caggcccttc
     9241 atgcacctgt cgtggttaat gccgctggga tctgggggca acacattgcc gaatatgccg
     9301 atctgcgcat tcgcatgttc ccggcgaaag gatcgctgct gatcatggat caccgcatta
     9361 accagcatgt gatcaaccgc tgccgtaaac cttccgacgc cgatattctg gtgcctggcg
     9421 ataccatttc gctgattggt accacctctt tacgtattga ttacaacgag attgacgata
     9481 atcgagtgac ggcagaagag gttgatattc tgctgcgtga aggggaaaaa ctggcccccg
     9541 tgatggcgaa aacgcgcatt ttgcgggcct attctggcgt gcgcccgctg gttgccagcg
     9601 atgacgaccc gagcggacgt aacgtcagcc gtggcatcgt gctgctcgac catgctgaac
     9661 gcgatggtct ggacggattt atcaccatca ccggtggcaa actgatgacc tatcggctga
     9721 tggctgaatg ggctaccgac gcggtatgcc gcaaactggg caacacgcgc ccctgtacga
     9781 ctgccgatct ggcactgcct ggttcacaag aacccgctga agttaccttg cgtaaagtca
     9841 tctccctgcc tgccccgctg cgcggttctg cggtttatcg tcatggcgat cgcacgcctg
     9901 cctggctgag cgaaggccgt ctacaccgta gcctggtatg tgagtgcgaa gcggtaactg
     9961 cgggtgaagt gcagtacgcg gtagaaaatt taaacgttaa tagcctgctg gatttacgcc
    10021 gtcgtacccg tgtggggatg ggcacctgcc agggcgaact ctgcgcctgc cgcgctgccg
    10081 gactgctgca acggtttaac gtcacgacgt ccgcgcaatc tatcgagcaa ctttccacct
    10141 tccttaacga acgctggaaa ggcgtgcaac ccatcgcctg gggagacgca ctgcgcgaaa
    10201 gcgaatttac ccgctgggtt tatcagggac tgtgtggtct ggagaaggag cagaaagatg
    10261 cgctttgata ctgtcattat gggcggcggc ctcgccggat tactctgtgg cctgcaactg
    10321 caaaaacacg gcctgcgctg tgccattgtc acgcgtggtc aaagcgcgtt gcatttctca
    10381 tccggttcgc tggatttgct gagccatctg ccagatggtc aacccgtggc agacattcac
    10441 agtggactgg aatctttgcg tcagcaggca cctgcacatc cttactctct tctcgggcca
    10501 caacgcgtgc tcgatctcgc ttgccaggcg caggcattaa tcgctgaaag cggtgcgcaa
    10561 ttgcagggca gcgtagaact ggctcaccag cggattacgc cgctcggcac tttgcgtgca
    10621 acctggttaa gttcgccaga agttcccgtc tggccgctac ccgcgaagaa gatatgtgta
    10681 gtggggataa gcgggctgat ggatttccag gcgcatcttg cggcggcttc gttgcgcgaa
    10741 ctggatcttt cggttgaaac cgcagaaata gagctgccgg aactggatgt cctgcgcaat
    10801 aacgccaccg aatttcgcgc ggtgaatatc gcccgtttcc ttgataatga agaaaactgg
    10861 ccgctgttac ttgatgcgct tattcctgtc gccaatacct gcgaaatgat cctgatgccc
    10921 gcctgcttcg gcctggccga tgacaaactg tggcgttggt tgaatgaaaa actaccttgt
    10981 tcactgatgc ttttgccaac gctgccgcct tccgtgctgg gcattcgtct gcaaaaccag
    11041 ttacagcgcc agtttgtgcg ccaaggtgga gtgtggatgc cgggcgatga agtgaaaaaa
    11101 gtgacctgta aaaatggcgt agtgaatgaa atctggaccc gcaatcacgc cgatattccg
    11161 ctacgtccac gtttcgcggt tctcgccagc ggcagtttct ttagtggcgg actagtagcg
    11221 gaacgtaacg gcattcgcga gccgattctc ggccttgatg tgctacaaac cgccacgcgg
    11281 ggtgaatggt ataagggcga tttttttgcg ccgcaaccgt ggcagcagtt cggtgtaacc
    11341 actgatgaga cgctacgccc gtcgcaggca gggcaaacca ttgagaacct gtttgccatc
    11401 ggttcggtgc tgggcggatt tgatcccatc gcccagggat gcggcggcgg tgtttgtgcc
    11461 gtcagtgctt tacatgccgc tcaacagatt gcccaacgcg caggaggcca acaatgaacg
    11521 acaccagctt cgaaaactgc attaagtgca ccgtctgcac caccgcctgc ccggtgagcc
    11581 gggtgaatcc cggttatcca gggccaaaac aagccgggcc ggatggcgag cgtctgcgtt
    11641 tgaaagatgg cgcactgtat gacgaggcgc tgaaatattg catcaactgc aaacgttgtg
    11701 aagtcgcctg cccgtccgat gtgaagattg gcgatattat ccagcgcgcg cgggcgaaat
    11761 atgacaccac gcgcccgtcg ctgcgtaatt ttgtgttgag tcataccgac ctgatgggta
    11821 gcgtttccac gccgttcgca ccaatcgtca acaccgctac ctcgctgaaa ccggtgcggc
    11881 agctgcttga tgcggcgtta aaaatcgatc atcgtcgcac gctaccgaaa tactccttcg
    11941 gcacgttccg tcgctggtat cgcagcatag cggctcagca agcacaatat aaagaccagg
    12001 tcgctttctt tcacggctgc ttcgttaact acaaccatcc gcagttaggt aaagatttaa
    12061 ttaaagtgct caacgcaatg ggtaccggtg tacaactgct cagcaaagaa aaatgctgcg
    12121 gcgtaccgct aatcgccaac ggctttaccg ataaagcacg caaacaggca attacgaatg
    12181 tagagtcgat ccgcgaagct gtgggagtaa aaggcattcc ggtgattgcc acctcctcaa
    12241 cctgtacatt tgccctgcgc gacgaatacc cggaagtgct gaatgtcgac aacaaaggct
    12301 tgcgcgatca tatcgaactg gcaacccgct ggctgtggcg caagctggac gaaggcaaaa
    12361 cgttaccgct gaaaccgctg ccgctgaaag tggtttatca cactccgtgc catatggaaa
    12421 aaatgggctg gacgctctac accctggagc tgttgcgtaa catcccgggg cttgagttaa
    12481 cggtgctgga ttcccagtgc tgcggtattg cgggtactta cggtttcaaa aaagagaact
    12541 accccacctc acaagccatc ggcgcaccac tgttccgcca gatagaagaa agcggcgcag
    12601 atctggtggt caccgactgc gaaacctgta aatggcagat tgagatgtcc acaagtcttc
    12661 gctgcgaaca tccgattacg ctactggccc aggcgctggc ttaaactccc ttctgatgcc
    12721 cggtaagcat gtggttaccg ggcatttttg cgtacacgat tccgtgccca atgtatgcgt
    12781 tgcaacgcag tgaaaattcc tctgaaaacg tctcgcaaag gctgaaactg gcagatgtca
    12841 aaggcctggg ataaccgtaa tgtcgcgtca tcataaatat caggtgacgg acaaccatga
    12901 ccgaatcaac aacctcctcc ccgcatgatg cggtatttaa aacctttata ttcacacccg
    12961 aaaccgcacg ggattttctc gaaatacatt taccagaacc actgcgcaag ctttgcaacc
    13021 tgcaaacctt acgcctggaa cccactagtt ttattgaaaa aagtttacgc gcttactact
    13081 cggatgtttt gtggtccgtg gaaaccagcg acggtgacgg ctatatctac tgcgtgattg
    13141 aacatcaaag ctctgcagaa aagaatatgg cttttcggcc aatgcgctat gccactgccg
    13201 ccatgcagag tcacctagat aaaggctatg acagagttcc gctggtggtg ccattgctgt
    13261 tttatcatgg cgaaacatcg ccctacccgt actcacttaa ctggctggat gagtttgacg
    13321 atccgcaact tgcccggcag ttgtacaccg aagcttttcc gttggtggat atcaccatcg
    13381 tacctgacga tgagatcatg caacatcggc gtatagctct gctggaactg attcaaaagc
    13441 atattcgcga ccacgattta atcggcatgg tcgacaggat caccacgctt ttggttagag
    13501 gcttcactaa tgacagccag ctacaaacac tgtttaatta tctgctgcaa tgcggcgata
    13561 cctcccgttt cacccgtttt attcaggaga ttgccgaacg ttcaccacta caaaaggaga
    13621 gattaatgac tattgctgaa cggctacggc aggaaggaca tcaaattggc tggcaagaag
    13681 gtaaattagt aggtttgcag caaggtaaat tagaaggttt gcaggaaggt atgcatgaac
    13741 aagccattaa aattgctttg cgcatgctgg aacagggcat tgatcgtgac caagtgctcg
    13801 cggccaccca gctaagcgaa gccgatctgg cagcgaataa ccactaatta acacaggccc
    13861 acagccgatc cccatgggcc tttgatatca ataactacct tttatgcgtg gcccattttt
    13921 gcctgattta aacatcgcca gtcgttgatc cagggcatcg ctgtagagca tcgtgtcaac
    13981 gccaacagcg acaaagttcg ctccccacgc caggcattgc tgcgccatat caggagccac
    14041 agccagaaaa ccagccgctt tacccgcagc acggatccgc cgaatgctgg tttcaataat
    14101 tcgctgcact tccgggtgcc cggcgttatc cgggtagccc aacgacgcag aaagatccgc
    14161 tggtccaata aacacgccat caatcccttc gacgtcgagg atctcgtcca ggttatccag
    14221 tgccgtttta ctttccacct gcactaacag acaaagcgaa tcgttgactt gcgccatgta
    14281 attctcaatg cgtccccagc gcgcagcccg tgccacactg gccccaacac cacgctcacc
    14341 gtagggaggg ttggacaaat tttgccggag ggggaatgtg ccggtgcagt aaaccggcac
    14401 agagtggcga aatcagacgg ataaagtcat ggactcaaca acctcaatcc agccatgctc
    14461 actagcgatg tcctggccgt ttaaccaacg gcgtagcata ttcagtgcca tcattgcgca
    14521 cacttcctga cggatagcca ggctgtagcg tgtagtgctg aaacgcacac gcagagcgaa
    14581 agtgccgtct ggcgtggcta gcgcaaagtt gagatgctcg ttctcgaaac ccgaaacagc
    14641 cagtgccagc ccggcaaaat ggttggcccg ccgttctgta atccagtgcg cagtttgcgc
    14701 cagggtttct tcctgtgaag gaatcacttc acacgccagc aatggagcac ctgcgcgaga
    14761 aagttgcaaa gccaataaac caccggtgaa ttgctcgctc aacgtcaggc tgaactggcg
    14821 gttttgcaat tcgcgactga tctgcgcggg cagtccttcg gtgccttcga aaatcacgct
    14881 ctgtccggca acacgtttaa cgtccagcca cagtttttcc atcgcctgtt gctcgcttgc
    14941 cggtccggtg agtttcagtt cgatgatcgg cattgaggag cgatagccca ttgttacgcc
    15001 cggcggcagt tgtagagtgt ccaggctttg tgccagatcg ctttccgaac gaccaaaggt
    15061 agttaaacgc agacaaaccg gcggctgtgg taaagaaaaa cgctcgcgca ggcgcggcag
    15121 gatttcgtgc tcgaccatca ccttaaattc tgacggtacg ccgggagtaa agaacatcag
    15181 gcaacgatta agctgcacgg caaaaccaca ggcggtgcct accgggttat tgataaattc
    15241 agcactggca ggcagctccg cttgtttacg gttgctcggt gccattactc gtccacgttc
    15301 gtggaaatag cgttccatct ctttgagcca ggcttcatgc agcaccaggc cttcaccttt
    15361 tgctgtcgca gcggcgagtg cgcttaaatc atcgctggtc ggtcccagcc cgccgttaac
    15421 gatcagcaca tcggcgtgct gactacgttc gcgaagaatg gtgactaagt catcaaggtt
    15481 atcccccacc gtattgcggc gagataatgg cagcccctga tgaaagaaaa aatcggccag
    15541 ccaggcagcg ttagtgtcaa cgatttgccc gtataacact tcatccccgg tggatagcat
    15601 ttccactttt aacatggtta tctcctgctt ttgttatcga atcactataa cgcaaaggga
    15661 gataaggcgt agtaagaaac tgaatgccgc gccggaaggg atcagaaact ggcgttaacc
    15721 ccaacataca ggccgtcagc cacagcgttg tcgcggttac cgtctttacc cgacagattc
    15781 aggtagcgat aacccgcctc aatactgact ggacgcataa tggtgtaacg cgcgccagca
    15841 ttcgcttcct cataactttg aataccgctg gagagcgaat ccggagagta gtaatactcg
    15901 ccaaacaaac ggaagctgtt gccaattttc cactgcaaac cacctcctac cgctgcggca
    15961 taaccttcat cgccgtaatt cgggttggtg tacacgcctt ttccgccaac ggtcgccatt
    16021 aacggcccga gaggcagatt caaccccagc cccacgcccg cgacgtcacc gtcgtcgtcg
    16081 ttatgtgtcc agttaccgct taaagccagg cccgtcgatt cagtcccaaa accgacacca
    16141 atattggtgt attctttacc cgcctggccg ctgatgctca ttgcattgac cgatgcagaa
    16201 accaacagca ttccggccag acctgctagc gcaatttttt tcattatccc ctttcccaca
    16261 agcaattaat caaaattccc aaagctgcgg gattgtactg cctggcacag aggattcaat
    16321 gagcgaaaaa agggatctag tcacttattg taactaaata cttcttaaat aattaaacgc
    16381 cgaaaaaata aacacacttt accgatacac acaaaaataa taataagtgc agttatcatt
    16441 gcaaaagaga ttactgtcac tttcacatat ttctgagtta ccgtattctc atggtttctt
    16501 tttatttata aggaataccc ctgtgcgaca acggactatt gtatgccctt tgattcaaaa
    16561 tgatggtgct tatttgctgt gcaaaatggc cgacgatcgc ggcgttttcc ccggtcaatg
    16621 ggcgctttcg ggtggcggcg tggagtctgg cgaacgaatt gaagaggcac tacgccgcga
    16681 aattcgcgaa gaactgggag aacagctgct tttgacagaa atcacgccgt ggaccttcag
    16741 cgatgatatt cgcaccaaga catatgcaga tggtcgcaag gaagagattt atatgattta
    16801 cctgattttt gactgcgttt ctgccaaccg agaagtgaaa ataaacgaag agtttcagga
    16861 ctacgcgtgg gtaaaacctg aagatctggt gcattatgat ttaaatgttg ccacccgaaa
    16921 aacgttacgt ttgaaaggtc ttctttaaca atgatgacag ccatcactgg ctgtcagatt
    16981 gaaaatttag tggttaacga attccccatc cagataaact ttgccttttt ccacatgcat
    17041 gactaaacca tccagataat caggtttaaa tatcgcatca cgcttatttt tagcaatata
    17101 tgtcaggcaa tgattatggg tgaaaataac gatattttta tcaggcgctt tgttttgtaa
    17161 gtccttaatt gcactataaa tctcattacc gcactgaaga agtcgtttat ctaccgtcag
    17221 ctttttaccc gcggaaaacc aggtcgccga ctgaatggtc cggacggtat tactggaata
    17281 aagatcgaaa tcagagatgt cagcgctaaa agcgttgccc agttcacggg catcctgggt
    17341 acctttaacc gtaatacctg ttttatctga caagcattga ttggttgaac gatcgcaacg
    17401 ttcagcatgg cgaaacaaaa cgacaaccgg gtgctgctgt gccagtctgg ccaatgtttt
    17461 attgtcgata cgtggcaaac cattgctact ccaggcggca tgagtaccca gtccggcaat
    17521 tgcagcgagc gccagtaaaa tgatgaaata tttttttgac ttcaatgatg agcggcaaaa
    17581 agctaacacg taaactccac ctatagacaa gcgcaaccag acaattaccg tgaaattgag
    17641 cgacatttct ggcgataatt agcagttggt gtaatattaa aaatcctacg atgtcggcaa
    17701 aatgcctcaa atttttgcca aatgcaaagc ctaaataaga aaaaatataa aaatttcaat
    17761 atttacgtct aatattagtt tcttaaggtt aagttaatat tctatcctta aaatttcgct
    17821 ccaaatggca aaatatacac aacactcttt atagcaaata taagtggaca ggtattcaat
    17881 ggcggaagga aaagcaatgt cagaattttt gcctttttcg cgaccagcaa tgggcgtgga
    17941 ggaactcgct gcagttaaag aggttctcga atccggttgg atcacaaccg gtccgaagaa
    18001 tcaggcgctt gagcaagcct tttgccagtt gacgggaaat cagcatgcca tcgcggtcag
    18061 ttcagccacc gccggaatgc atatcacgct aatggcgttg gaaattggca agggcgatga
    18121 agtgattacg ccttccctga cctgggtttc aaccctcaat atgatttcct tgctgggtgc
    18181 aacgccggta atggtggatg tcgaccgcga tacgctgatg gtcacgcctg aagcgatcga
    18241 atcagccatt acgccacgca ctaaagccat cattccggtg cattatgccg gtgcgccagc
    18301 agatattgac gccattcgcg ccattggcga acgttacggc atcgcagtta tcgaagatgc
    18361 tgcccatgcc gtcggtacgt attacaaagg gcgacatatt ggcgcaaaag gtaccgctat
    18421 tttttcattt catgccatta aaaatattac ctgtgctgaa ggtggcctga ttgtaactga
    18481 taatgaaaac cttgcccgcc agctacggat gctgaaattt cacggtctgg gtgtcgatgc
    18541 ctatgacaga caaacctggg gccgtgcacc gcaggctgaa gtcttaacac cgggctataa
    18601 gtacaatctg accgatatta acgccgcgat tgccctgaca cagttagtca aattagagca
    18661 cctcaacacc cgtcggcgcg aaattgccca gcaatatcag caagcactgg cagctctccc
    18721 ctttcagcca ttaagccttc ccgcctggcc gcacgttcac gcctggcatc tgtttattat
    18781 tcgtgtcgat gaacaacgtt gtggtatcag tcgcgatgcg ttgatggaag cgttaaaaga
    18841 aagaggcatt ggtaccgggt tacatttccg cgccgctcac acacaaaaat attatcgcga
    18901 gcgttttccc acgctgtcgt taccgaatac cgaatggaat agcgaacgca tctgttcgtt
    18961 gccgctgttc ccggatatga ctaccgccga tgccgaccgc gtcatcacag cccttcagca
    19021 actcgcagga caataagcca tgtttgaaat ccaccctgtt aagaaagtct cggtggttat
    19081 tcccgtttat aacgagcagg aaagcttacc ggaattaatc aggcgcacca ccacagcctg
    19141 tgaatcgttg gggaaagagt atgagatcct gctgattgat gacggcagta gcgataattc
    19201 cgcgcatatg ctggtcgaag cctcacaagc ggagaacagc catattgtgt ctattttgat
    19261 taaccgcaat tacgggcaac attcagcgat tatggcggga ttcagtcacg ttactggcga
    19321 cttaattatt acccttgatg ccgatctcca gaatccgcca gaagaaatcc cccgcctggt
    19381 ggcaaaagcc gatgaaggtt acgacgtggt agggactgta cgccagaacc gccaggacag
    19441 ctggtttcgt aaaacagctt cgaagatgat taaccggctt attcagcgca ccactggcaa
    19501 agcgatgggt gactacggtt gtatgctgcg cgcctatcgc cgtcatattg tcgatgcgat
    19561 gttgcactgc catgaacgca gcacctttat cccgattctg gcgaatatct tcgcccgccg
    19621 tgcaattgaa attccagtac atcatgccga gcgtgagttt ggtgaatcca aatacagttt
    19681 tatgcgcctg attaatttga tgtacgacct ggtgacctgc cttaccacaa cgccgctacg
    19741 tatgctgagt ctgctcggca gcattattgc gattggaggt tttagcattg cggtgctgct
    19801 ggtgatttta cgcctgacct tcggaccaca atgggcggca gaaggcgtct ttatgctatt
    19861 tgccgtgctg tttactttta ttggcgctca gtttatcggc atgggattac tcggtgaata
    19921 tatcggcagg atctacaccg atgtccgcgc ccgcccccgc tattttgttc agcaagttat
    19981 ccgtccatcc agcaaggaaa atgaataatg aaaaccgtcg tttttgccta ccacgatatg
    20041 ggatgcctcg gtattgaagc tctgctggct gccggttacg aaattagcgc catttttacc
    20101 cataccgata atcccggtga aaaagccttt tatggttcgg tggctcatct ggcggcggaa
    20161 agagacattc cggtttatgc gccggataac gttaatcatc cgctgtgggt ggaacgcatt
    20221 gcccaactgt caccagaggt gattttctct ttttattatc gccatcttat ttgcgacgag
    20281 atttttcagc tcgctcccgc aggtgcattt aatctgcacg gttcgctgtt accaaaatat
    20341 cgtggtcgcg cgccgctgaa ctgggtgctg gtgaacggtg aaacggaaac gggcgttaca
    20401 ttgcaccgaa tggtaaacgt gccgatgctg gggccattgt ggcgcaactg cgcgttgcca
    20461 ttgcgccaga cgatatcgct attacgctgc atcataaatt gtgccatgcc gcgcgccagc
    20521 tactggaaca gacattaccc gccattaaac acggtaatat tctggaaatc gcccagcgcg
    20581 aaaacgaagc cacctgtttt ggtcgcagaa cgccggatga cagtttcctt gaatggcata
    20641 aaccggcatc cgtactgcac aacatggtac gtgccgttgc cgatccgtgg ccgggtgcct
    20701 tcagctatgt tggcaatcag aaattcaccg tctggtcgtc gcgtgttcat cctcatgcca
    20761 gcaaagcaca gccggggagc gtgatttctg ttgcgccact gctgattgcc tgtggcgatg
    20821 gcgcgctgga aatcgtcacc ggacaggcgg gcgacggcat tactatgcag ggctcgcaat
    20881 tagcgcagat gctgggcctg gtgcaaggtt cacgcttgaa tagccagcct gcctgcaccg
    20941 cccgacgccg tacccgggta ctcatcctcg gggtgaatgg ctttattggc aaccatctga
    21001 cagaacgcct gctgcgcgaa gatcattatg aagtttacgg tctggatatt ggcagcgatg
    21061 cgataagccg ttttctgaat catccacatt ttcactttgt cgaaggcgat atcagtattc
    21121 attccgaatg gattgagtat cacgtcaaaa aatgtgatgt cgtcttgccg ctggtggcga
    21181 tagccacgcc gattgaatat acccgcaacc cgctgcgcgt atttgaactc gattttgaag
    21241 agaatctgcg cattatccgc tactgcgtta agtaccgtaa gcgaatcatc ttcccgtcaa
    21301 cttcagaagt ttatgggatg tgtagcgata aatacttcga tgaggaccat tctaatttaa
    21361 tcgtcggccc ggtgaataaa ccacgctgga tttattcggt gtcaaaacaa ttacttgatc
    21421 gggtgatctg ggcctatggc gaaaaagagg gtttacagtt caccctcttc ctcccgttta
    21481 actggatggg accacgactg gataacctta atgcagcgcg aattggcagc tcccgcgcta
    21541 ttacgcaact cattctcaat ctggtagaag gttcaccaat taagctaatt gatggcggaa
    21601 aacaaaaacg ctgctttact gatatccggg acggtatcga ggcgttgtac cacattatcg
    21661 aaaatgcggg aaatcgctgc gacggcgaaa ttatcaacat tggcaatcct gagaacgaag
    21721 cgagcattga ggaactgggc gagatgctgc tggcgagctt cgaaaaacat ccgctgcgcc
    21781 atcatttccc accgtttgcg ggctttcgcg ttgtcgaaag tagctgctac tacggcaaag
    21841 gatatcagga cgtagagcat cgtaaaccga gcatccgcaa tgcccaccgc tgcctggact
    21901 gggagccgaa aattgatatg caggaaacca tcgacgaaac gctggatttc ttcctgcgca
    21961 ccgttgatct tacggataaa ccatcatgac caaagtaggc ttacgcattg atgtcgatgc
    22021 ctttcgtggc acccgtgaag gcgtgccgcg tctgctggaa atcttgagta agcataatat
    22081 tcaggccagc atttttttca gcgtcggccc ggacaatatg ggccgccatc tctggcgact
    22141 ggtgaagcca cagtttttgt ggaagatgct gcgctcaaac gcggcatcgc tttatggctg
    22201 ggatatttta ctggcaggta cggcctggcc aggtaaagag attggtcatg ccaatgccga
    22261 tatcattcgt gaagcggcta aacatcacga agtcggtctg cacgcctggg atcaccatgc
    22321 ctggcaagcc cgtagcggta actgggatcg gcaaacaatg atcgacgata ttgcacgcgg
    22381 tcttcgcact ctggaagaga ttatcggtca accggtaacc tgttctgccg ctgcgggctg
    22441 gcgtgccgac cagaaggtga tcgaagcaaa agaagcgttc catttgcgct acaacagcga
    22501 ttgtcgtggg gccataccgt tccgtccact gcttgaatca ggaaaccctg gcactgcgca
    22561 aattccggtg accttaccca cctgggatga agtgattggt cgggatgtga aagcagaaga
    22621 ttttaacggt tggttactca accgcatcct gcgagataaa ggcacgccgg tttataccat
    22681 tcatgcagaa gttgaaggct gcgcttatca gcataatttt gtggatctcc tcaaacgcgc
    22741 agctcaggaa ggcgtgacat tttgcccttt aagcgaactg ttatcagaga cgttgccgct
    22801 cggacaagtt gttcgcggaa atattgccgg acgtgaaggc tggctgggtt gccaacaaat
    22861 tgcgggtagt cgctgatgaa atcggtacgt taccttatcg gcatcttcgc gtttattgcc
    22921 tgctattacc tgttaccgat cagcacgcgt ctgctctggc aacccgatga aacgcgttat
    22981 gcggaaatca gtcgagaaat gctggcatcc ggcgactgga ttgtgcccca tctgttaggg
    23041 ctacgttatt tcgaaaaacc cattgccgga tactggatta acagcattgg gcaatggcta
    23101 tttggcgcga ataactttgg tgtgcgggca ggcgttatct ttgcgaccct gttaactgcc
    23161 gcgctggtga cctggtttac tctgcgctta tggcgcgata aacgtctggc tctactcgcc
    23221 acagtaattt atctctcatt gtttattgtc tatgccatcg gcacttatgc cgtgctcgat
    23281 ccgtttattg cattctggct ggtggcggga atgtgcagct tctggctggc aatgcaggca
    23341 cagacgtgga aaggcaaaag cgcaggattt ttactgctgg gaatcacctg cggcatgggg
    23401 gtgatgacca aaggttttct cgcccttgcc gtgccggtat taagcgtgct gccatgggta
    23461 gcgacgcaaa aacgctggaa agatctcttt atttacggct ggctggcggt tatcagttgc
    23521 gtactgacgg ttctcccctg gggactggcg atagcgcagc gggagcctga cttctggcac
    23581 tattttttct gggttgagca tattcaacgc tttgcactag atgatgccca acatagagct
    23641 ccgttctggt actacgtgcc ggtcatcatt gccggtagcc tgccgtggct gggattactc
    23701 cccggtgcac tgtacacagg ctggaaaaac cgcaagcatt ccgcaaccgt ctatttgttg
    23761 agctggacga taatgccgct gctgtttttc tccgtcgcta aaggcaaatt gcccacctat
    23821 attctttctt gctttgcacc tctggcaatg ctgttggcgc attacgcgtt gctggcagca
    23881 aaaaataatc ctctggcgct gcggattaat ggctggatta acatcgcttt tggcgtcact
    23941 ggcattattg ccacatttgt gatctccccc tggggaccaa tgaacacgcc ggtgtggcaa
    24001 accttcgaga gctataaagt cttttgtgcc tggtcgattt tttcgctatg ggcatttttc
    24061 ggctggtaca ccttaacaaa cgtcgaaaag acctggtctt tcgccgcgct ttgcccgctg
    24121 gggctggcgt tgctggtagg attttcaatt cctgacagag tcatggaagg aaaacatccg
    24181 caattttttg tcgagatgac acaagaatca cttcagccaa gccgctatat tcttactgat
    24241 agcgtcggtg ttgccgcagg tctggcatgg agcctgcaac gcgatgacat catcatgtat
    24301 cgccagacag gtgagttgaa atacggcctt aattatccgg atgcgaaagg gagatttgtc
    24361 agcggtgatg agttcgcaaa ctggcttaat caacatcgtc aggaggggat tattacactc
    24421 gtgctttcgg ttgaccgcga tgaagatatc aacagtctcg ccattccgcc cgcagatgcc
    24481 atcgttcgtc aggagcgtct ggtgctgatt cagtatcgtc ccaaatgatc tggctaacat
    24541 tagtctttgc cagcttgctt agcgttgccg ggcagttgtg tcagaaacag gcaacctgct
    24601 ttgtggcgat aaacaaacgg cgcaaacata tcgtgctgtg gctgggactg gcgctggctt
    24661 gtcttggtct tgccatgatg ctctggctgc tggtcttgca gaacgtaccg gtaggcattg
    24721 cctacccgat gttaagtctg aattttgtct gggtgacgct ggctgcagta aaactgtggc
    24781 acgaaccggt atcgccgcgt cactggtgtg gggtggcgtt cattattggt ggcattgtga
    24841 tcctcgggag tacggtgtaa tgtgcctgat atggggatta ttcagcgtca ttattgcctc
    24901 agttgcgcaa ttaagcctgg gttttgcggc aagtcatctg ccgccgatga cgcacttatg
    24961 ggattttatc gcggctctgt tagcttttgg cctggatgcc cggatactgt tactcgggct
    25021 gctgggttat ctgctttccg ttttttgttg gtataaaacg ctacataaac ttgcgctaag
    25081 caaagcctat gccttgctca gtatgagtta tgtgctggta tggattgctt caatggtact
    25141 tcccggctgg gaagggactt tttcgctgaa agcactactg ggagtagcct gtattatgag
    25201 cgggttgatg ctgattttcc tgcccacgac aaaacaacgt tactgagttt tccctgccgc
    25261 tgtacactgc cgttcccact catcggggct ataactgctt gccgagagga ctttcaccgt
    25321 atgcagcttt tcacgattaa tacaataaag cgcattctgc aaaggcgaga gtaaatcccc
    25381 cactttcacg gcgaagtcac tcttcacttc ggcgatcatt tttatcgcgc cgccagcatc
    25441 gcagagcata agtacatgtc tgttatcttg tttattgcag cacgattttt tgtgccagcc
    25501 attccattgc attatcctgt ttgctaagag ttttcacatc aatttacaga ttaacagata
    25561 aactctttat caacggcaat ggaatggcag aaatgagaag tgaaacggag ggttaattat
    25621 tgctgacgtt gcacccactc ttttagcgcc tgacgtgaaa ttttaatacc gccgtttttc
    25681 agctccggcg gtaaagtcag ccagcgcacc ggttgttgaa aacgagccag cttatctttc
    25741 acccattcgc caagatcaac ggtttgctgg tcatactcca ccaccgctac cggccgatga
    25801 ccaaactcct tgtcggcaac ggaaacgata aacacctgca aaaccgcagg atgtgcagca
    25861 ataacacgct cgacttcttc cggctgaata ccctcaccgc cactgaagaa cagattgtct
    25921 aaacgtccga caatggtcag cttgccatta tgcatctcac cgcgatcgcg cgtagcgtac
    25981 cagccttcgt cattaaccag tgaaaccagt tgcccgttac gccagtaacc ttctgccata
    26041 ctggcagccc gcagccacac ttcattatta acgattttca cttcccgacc cggcagcggc
    26101 aaaccaacgt ctgccaggcc gtcggcttct ttcgcacata ccgtggaggc aaactcggtc
    26161 agaccatagc cgcaaaagca acgaatcccc tgctcgcgcg cctgttccgt caactcgacc
    26221 gggatagccg cgccgccaag taacaccgct ttcagggaaa cggaactgcg gttaaccagc
    26281 aaacgccaga gttgtgttgg caccagtgaa gcgtgagtac agcctgccag catttgctcc
    26341 aatggctgtt tatcacgcac cgtcatccgc gcaccagcgt ataaccagcg ccacataatt
    26401 ccctgaccgg agacgtgaaa cagcggtaaa gagagcaacc agtcatcgtg atcgccaaac
    26461 ggaatcagcg ataacacacc ttgcgcactg gcaagatggg cctgataagt atggacagcg
    26521 gctttcggca aaccggtaga acccgaggtc aacgtcattg agcacagacg cgtcggctgc
    26581 cacgcagcgg catgtacgcc ttcaaccagc tgaatgtgca gcgacgttaa tgccggaaac
    26641 gtgttttccc catccggcac cagcgcaaat tgcagcgtca gattgggcag caattcttca
    26701 agcaacggtt gcggcagctg agggttcacg ggcaacaccc gcgccccgca ttgcagtaac
    26761 gccagccagg cgagcagcgt ttgcggcgta ttccacgccc gcaacatcac gccgctgccc
    26821 tcaaccaccc cctgcgccgc aaagcccgta gctaactcat cgacgcgagc acaaagctcg
    26881 cgccagttga gttgctcgtc attaagacgt aaggcgatgg cttctccccg cacttgccgc
    26941 cagtgacgcc acggccagtc agagaagatc ataacaactg ctcctgtaca tcagcgtcca
    27001 ctaacggcag tgggctcccc ggccagcgac gaacctgctg cgcctgcatc agatccagcg
    27061 tgtccagtcc cggaatggtg tctggcgtta accaggcagc aatccgcgcc agttgcgtta
    27121 gacctaagct cgactcaatg gaagaactga tcaccgccgt cagccccagc gcatgcgccg
    27181 cctgtacctg ctcgcgtact ttttccagac tgcccgtgag cgtgggtttg ataactaccg
    27241 cgcgcacgcc ctcttcagcc acaaaggcaa aatccggctc gcgcaggctt tcatcccagg
    27301 caatggcaat gccggtttca cgggcaaacg ctcgcgaatc atcgcgggtt ttgcacggct
    27361 cttcgagaaa cgcgatgcgg tggcgataat ccgggttaac atatttggca aactgctgac
    27421 ctttcagcgg tgtccaggcg cgatttgcgt caagacgcaa atgcagatcc ggaattgcct
    27481 ccagcaacag attcaccacc atgccgtcgc gcaccgcttc gtacaatccc actttgacct
    27541 tcgccacttt ctcgcccggc atatcggcaa gtttgaggat cagatcgtcc ggatcgccat
    27601 tacacagcgg tgccgcacgg tagttggctg cttgcggcaa cgtatctgtc agttctgcca
    27661 atgcacagct tacgccaaag gccacggaag gcatctgcgg tagctcgcaa tcgcctgcca
    27721 gccagttatt tacccaggca agcagcacgc tttgcgcctc ttcccagttt tcctggctga
    27781 agcccggcag tggggagatc tccccccacc cttcgcgctc gccctcacgc aggcaaacgt
    27841 acagcccgtc gcgggttttt aaccgcctgt cgcgcagaac cacccccgcg tccatgggga
    27901 tctgccagcg gtatacctgc gcgctacgca ttacggattc cgtttaaatt tgctgaaatc
    27961 aggctgacgt ttctggttga aagcgttgcg accttcctga ccttcttccg tcatgtagaa
    28021 cagcatggtg gcgttgcccg ccagctcctg cagccccgcc tgcccgtcac agtcggcgtt
    28081 cagcgcagct ttcaggcagc gcagcgccat cgggctgttt tgcagcattt cgcggcacca
    28141 acggacggtt tctttttcca gatccgccag cggtaccacg gtgttgacaa ggcccatatc
    28201 cagcgcctgt tttgcgtcgt actgacggca caggaaccag atttctcgcg cttttttctg
    28261 cccgacgata cgagccatgt aggaagcgcc ccagccgccg tcgaaggaac cgactttcgg
    28321 gccagtctga ccgaagatgg cattatctgc tgcgatagtc aggtcgcaca tcatgtgcag
    28381 aacgtgacca ccgccgatgg agtagccagc caccatcgcg acaaccggtt tcggacaggt
    28441 acggatctga cgctggaagt ccagcacgtt caggtgatgt acgccggaat catctttata
    28501 gccgccgtaa tcaccacgca ctttctggtc accaccggag cagaacgctt tatcgcctgc
    28561 gccagtcaga atgatcacgc cgatattgtc gtcataacgc gcatctgcca gtgcctggat
    28621 catctctttt accgtcagag gacggaaggc attgcgcacc tgcggacgat taatggtgat
    28681 ttttgcgata ccgtcggtgg atttttcata acgaatgtcc tcgaaacctt cggagcagtc
    28741 gtgccattca accggtgcgt aaggcattgc ttcatcagga taaatcatat tgtgtccttt
    28801 agtcagaaac gcaagatctg cgccagactt gcgattacgc cagcgggatt ttcccgatgc
    28861 gcgttatgtc cggcgcgagg aatgacatgg cagtcggcag ccagttccgc cgccagggcg
    28921 cggaatttgc tgtcacgttc accacataaa taataaaacg caaatgtgcg ggcgctaagg
    28981 ttagcacgta aatcaggctg gacggcgaga gaagtcgcct ccagcatggc ggcaagggtt
    29041 gcgccattat tgttgctgcg cagcgccacc agctcccggc gttgatcgtc attgagcgag
    29101 gcaaaaacag gctgttgata ccagtcggca aataccgccg ttaacggttc tgtgcgaaaa
    29161 cgctgcgccc attggcgatc ggaacgctga cgttccgcac gttgttcagc attttgcagc
    29221 cccggatgcc cgccttcgac aataacccca caaagccccg ccagcccctg gcaagccgcc
    29281 atcatcgcca cccgtccgcc aagcgagtac cccaccagcc agaagtcaag gatgttgtaa
    29341 ctaaccaagg ttttacgcag taagtcggtg acatcatcaa atccatcgac gctaatcgcc
    29401 gccgaaccac catgacctag gagatcaaca tacaaccgtg agtagtcggc aaacgcctcg
    29461 cccacttctt gccattcgtg gcaatcgccg gaaaaaccgt gcaaaaacac cagccagggt
    29521 aaacctagtt ttccgtgttt tgcctgcgcg tgcaggatca taaatggctt acctgcgcca
    29581 gaagttgctg gagcgtttgc gcaccatcgg tgtcgttaac caccatttca atcaccgtgg
    29641 tggttggcgt gcgccaggcg tcggcaaatg ccgtttcaag ttcctgccag ttttgcggat
    29701 gatgatattt cagctcgaac atcgcggcgg cgtgctcaaa atggacgttt tgcggcatca
    29761 gatagaaacg ctcacgctcg ctttgcggcg ttggcaacag cgagaaaatt tgcccgccgt
    29821 tgttgttcac cacaattaat accagcggcg cagaaacctg acgcaataac gccagcgcgt
    29881 tgagatcgta aagcgcggag agatcgccca caatcgccag cgtcggtttg ccgcttgccc
    29941 gctgaacgcc tgcggcggtc gaaagcagtc cgtcgatacc gctggcccct cggttgctgt
    30001 acaccgggta acctgccgga agttgcgaaa gcgcatcaat cagacgtacc accaggctgt
    30061 taccaacaaa caattgcccc tgttcaggca gatagtcgct gatgcgatgc gccagttgcg
    30121 cttcgccaaa cgcatcacgg cgggcaataa ccgcctgcat tgcctgttcc gccaggcgcg
    30181 ggatttcaac gcaccagggc tggcgttttt ctgccggatg cagctccagc cagtcggcaa
    30241 tattggcaat taagcgacgt ccgcgatggt gtgccggatc aagtcgccct tcaatgtcat
    30301 caacaatcca gtactcttct ggttcacagc ttgcctgcca ttgcaggagc cgtttccccg
    30361 tcaggctact tcccagttgc accacaattt gcgcctgctg cagctcgctg gtcgctttgg
    30421 cattgcctaa ccaaagatcg gcacacggca gcggctgccc ggtttgtgac agcacatcgc
    30481 caatcagcgg ccagccaaga gtttgcgccc acagggcaac ttttttgccc tcttccgcac
    30541 tcatgcgccc ggcaaccacc acgccgcgct tttgtcgcca gaagaaccag tcgcgctgtt
    30601 tttcactttc cagacgaggc gcttcacgca gccacggttt gtcgtcctgc caccagtcac
    30661 ccagacgctg ttgccagcta agcccggtat cgtccatttc gccatacagc ggttcagcaa
    30721 acgggcagtt gatatggact ccccccgcat gaagcgtacc gagagcgtgg tcgatggtag
    30781 aaaccagcca acgtgcgggg atatcctggg tcgggcgcgg caatgaaata ctgtgcgtgg
    30841 ggtgagaggc gaacattccc ggctggcgaa ttgcctgatt cgcgccgcag tcaattagct
    30901 ccggcgggcg atcggcggtt aagagaatca gtttttctcc ggttaacccg gcttcaatca
    30961 gtgccggata gagatttgcc accgccgtgc cggaggtcac aatcaccgcc accggctgct
    31021 tgctgacttt cgccagcccc agcgccagat gccccaaccc acgctcatcg aaatgggtgt
    31081 ggtgaatgaa tgcggaattc tccgccgccg ctaacgttaa cggtgtagaa cgcgagcctg
    31141 gggcgataca gatgtgtctg acgccgtgac gcgttaatgc ttccagaatg accgccgccc
    31201 agcgtcggtt aaatgcgctt actgacatga gtttgtccgg tatcaatatt gcggctaagt
    31261 ataaggagct acaaaaatag attattgata tgaatcggta atgatgcgac tcattactat
    31321 tccatttgta ataaagtacg cagccctgcc gctttgttgt cgatttcctg ccactcttgc
    31381 tcggggtcgg aaccacggac aatgcccgcg ccagcatata atcgcacgac attgccgcta
    31441 atttttgctg agcgcaggga aacgcagaat tcgctttgtt gtaatgagag atagcccgca
    31501 gaaccggcgt accattctcg ggtgaacggt tcgtgacggg cgataaactg tcgcgccaga
    31561 tcgcgcggta agccagcaac tgctgccgtt ggctgcaact gatgtaaaca gatcacatca
    31621 tccgctttgt tgagtgaagt ccagatacag cggcgaagat gctgcacttt acgcagacgc
    31681 agtacctgcg gcggtaaaac atccagcgtc tgggtatcgg cctgtaatcg ttggcagata
    31741 tcttccacca ccagcatgtt ctcgcgctgg tttttatcat ccgccatcag ccactctcct
    31801 aactgctgcg cctgcttatc atcaggatga tttgctactg ttcccgccag cgcttcagta
    31861 cgcagcgctt tgtcacgccg ccgccataac cgttccggtg aagagccaag aaaagcattt
    31921 tcgccatcaa aggccatgta aaaatggtag caattcagat tcagtcgacg actggcagcc
    31981 atcatcgccg ccgcgttgac cggacttgcg aaatgcaggt cagttgcccg agcgagcacc
    32041 actttgtcga gctcaccttc ggcgatggtt ttcgttgcca gttcgattaa ttgcgtccag
    32101 cccgttttgt ccggccagtg ttgttctcgc gtggtggtta aatgtaaccc aggcaagggc
    32161 ttgatactca ccagtgtggc gataaattct tttgcctgaa tcgcatcgtg ctgaagggag
    32221 ctttcgctga atagcgtcag ccgcagcgtg gctttaccgc cacagcgtcg ccattccagg
    32281 cggggtaaaa gtaaattgcc ctgcgacggg tcaaaagcat tcagccccca aatgcgtaag
    32341 tcagcgtgtt ccgggtgctg gcgaagaaaa cgttgtgcct ggtccaacga cgtaaaacgg
    32401 gtaatcgcgc ccaggacggc agcttcttca tcaccattac gttgttgcca gtagaattgc
    32461 gggtacgttt gctgactggc cagccagctc aaggcatcaa aagcgtcttt gagagggaaa
    32521 ggaatatcga taacccgaat gccgggtgtc gccggaatct cttgcgacaa atggcgcagt
    32581 agattttcca gcgccgtagt aagtgattgc acgcggacct ctccctgtta aaagcctcac
    32641 attatacggg gtactacaaa aaaaatgcag taccccggtg tagggaggtt ttaacggcgt
    32701 gccagcaaca gtcctagtac cagcccgacg gccgcgccca caccaattcc ttgccagggt
    32761 ttttcgtgga cgtagtcatc agcacgataa acagcctgct tcgcccgata gtaataactg
    32821 tctgaagcct ggctaacccg ttttttcaca tcatccagcg ctttttctgc atgcgctttc
    32881 agctcaacat atttctgatc ggcgggatcg ccagaggagc ggagcacctc ttccagtgtt
    32941 tcactaagca gcgtcaggtc gtcatcgata cgtgtatcac caaactgatt agacattctc
    33001 gttctccatt tgcgtaaaac ctgtcactaa ctatagacaa gagttgttga ttacgcctga
    33061 attacctcgc gtgccatccc aatgtgtggg ataccatctt cttcatagac ctccgtcaca
    33121 gggataaaac cgaaactctg gtagaagttt tgcagatgcg cctgcgcccc gaggtaaaca
    33181 ggcttatcag gccagtgatg cgtacatgtt tcgagtgttt tactcatcag ctgctgcccc
    33241 actttttcac cacgcaacgc ttcactgaca atcacccgac ctataacgac cggctcaaga
    33301 tcatcatcac ttttcagaat cctcgcatac gccaccagtt catcattttt ccaaccgaga
    33361 atatggcgat tatcccccgt caaatcatca ccatcgatat cctgataagg gcaattctgc
    33421 tcgacaacaa acaccgcgca acgcagttgt aataaggcat aaagctggga aaccgataat
    33481 tcagagtgat gcagatcttg ccattcaatc ataatgctct ccttttagat gtatgcctta
    33541 tactagccgg aatatttttt gaaacgtgat gaactcatgg aattaatttt tttaggtact
    33601 tcagccggtg tgccaacccg cacgcgcaat gtcacggcaa tattgcttaa cctgcaacat
    33661 ccgacccaga gcggactttg gttgtttgac tgcggtgaag gcacccagca tcagctactg
    33721 cataccgcct ttaaccctgg aaaactggac aagattttta tcagtcacct tcatggcgat
    33781 catctttttg gtttacccgg cttgctgtgc agtcgttcta tgtcaggcat tatccaaccc
    33841 ttaacgattt atggtccgca aggtatccgt gaatttgtgg aaaccgcgct gcggattagc
    33901 ggctcatgga ccgattatcc gctggaaatt gtcgaaattg gcgctggcga aattctcgat
    33961 gatggcctgc gcaaagtaac cgcttatccg ctggaacacc cactggaatg ttatggctat
    34021 cgtattgaag aacatgataa accgggtgca ttaaatgccc aggcattaaa agctgctggc
    34081 gtgccgcctg gcccactgtt tcaggaatta aaagcgggca aaacaatcac gctggaagat
    34141 ggaaggcaga ttaacggcgc agattactta gctgctccag tgccaggtaa agcgctcgct
    34201 attttcggcg ataccggccc ctgcgatgcc gcacttgacc tggctaaagg tgtcgatgtc
    34261 atggtgcacg aagcgacgct ggatataacc atggaagcca aagccaatag tcgcggccat
    34321 agctctacac gccaggctgc gacactagcc cgtgaggctg gagtcggcaa gctaatcatt
    34381 acccacgtca gctcgcgcta tgatgacaaa ggttgtcaac acctgttacg tgaatgcagg
    34441 tcaattttcc cggcgactga actggcgaat gatttcaccg tgtttaacgt ttaactctgc
    34501 ttattattaa aaacagggtg aaacttgccc tgttatagca acccgcgcta catatatcca
    34561 cccacacagg catattgagc gatttttgat atttattcat ctccttacgc actatattca
    34621 gagcaataag ttgcctcttt tcatcttgat tcagcacaac ctttgcaact aaaaggagcc
    34681 catgatggtt acagttgtca gcaattattg tcaattatct caaacccaac tcagtcagac
    34741 atttgcagaa aaatttactg tgaccgagga attactgcag tctttaaaaa aaacagcgtt
    34801 atccggagat gaagaaagta ttgagttact gcataatatt gcgttaggtt atgatgaatt
    34861 tggaaaaaaa gctgggtaat gctgccaact tactgattta gtg
//
LOCUS       DAETQL010000039        34687 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.039, whole genome shotgun sequence.
ACCESSION   DAETQL010000039 DAETQL010000000
VERSION     DAETQL010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34687
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.039"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..2100
                     /locus_tag="K0W65_002044"
     CDS             <1..2100
                     /locus_tag="K0W65_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HBI3095311.1"
                     /translation="NFNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFN
                     AQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLL
                     GSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQ
                     AGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGN
                     LAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYE
                     SISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLS
                     ASRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPT
                     DYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVY
                     ATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQD
                     EESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGA
                     GNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTL
                     EINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLK
                     QPKLRGNVQTAQREVAP"
     gene            2097..3185
                     /locus_tag="K0W65_002045"
     CDS             2097..3185
                     /locus_tag="K0W65_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HBI3095312.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3273..4571)
                     /gene="exuT"
                     /locus_tag="K0W65_002046"
     CDS             complement(3273..4571)
                     /gene="exuT"
                     /locus_tag="K0W65_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HBI3095313.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5056..6468
                     /gene="uxaC"
                     /locus_tag="K0W65_002047"
     CDS             5056..6468
                     /gene="uxaC"
                     /locus_tag="K0W65_002047"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBI3095314.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6483..7970
                     /gene="uxaA"
                     /locus_tag="K0W65_002048"
     CDS             6483..7970
                     /gene="uxaA"
                     /locus_tag="K0W65_002048"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBI3095315.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8053..8604
                     /locus_tag="K0W65_002049"
     CDS             8053..8604
                     /locus_tag="K0W65_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBI3095316.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8609..9853)
                     /gene="sstT"
                     /locus_tag="K0W65_002050"
     CDS             complement(8609..9853)
                     /gene="sstT"
                     /locus_tag="K0W65_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBI3095317.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10449..11414)
                     /gene="alx"
                     /locus_tag="K0W65_002051"
     CDS             complement(10449..11414)
                     /gene="alx"
                     /locus_tag="K0W65_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBI3095318.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11685..12683)
                     /locus_tag="K0W65_002052"
     CDS             complement(11685..12683)
                     /locus_tag="K0W65_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBI3095319.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12762..13454)
                     /locus_tag="K0W65_002053"
     CDS             complement(12762..13454)
                     /locus_tag="K0W65_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBI3095320.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13531..14034)
                     /locus_tag="K0W65_002054"
     CDS             complement(13531..14034)
                     /locus_tag="K0W65_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBI3095321.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14119..15255
                     /gene="rlmG"
                     /locus_tag="K0W65_002055"
     CDS             14119..15255
                     /gene="rlmG"
                     /locus_tag="K0W65_002055"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBI3095322.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15540..15854
                     /gene="higB"
                     /locus_tag="K0W65_002056"
     CDS             15540..15854
                     /gene="higB"
                     /locus_tag="K0W65_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HBI3095323.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15851..16267
                     /gene="higA"
                     /locus_tag="K0W65_002057"
     CDS             15851..16267
                     /gene="higA"
                     /locus_tag="K0W65_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HBI3095324.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16312..18330)
                     /gene="fadH"
                     /locus_tag="K0W65_002058"
     CDS             complement(16312..18330)
                     /gene="fadH"
                     /locus_tag="K0W65_002058"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBI3095325.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18556..20907)
                     /gene="ygjK"
                     /locus_tag="K0W65_002059"
     CDS             complement(18556..20907)
                     /gene="ygjK"
                     /locus_tag="K0W65_002059"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBI3095326.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20924..21343)
                     /locus_tag="K0W65_002060"
     CDS             complement(20924..21343)
                     /locus_tag="K0W65_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095327.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21321..21674)
                     /locus_tag="K0W65_002061"
     CDS             complement(21321..21674)
                     /locus_tag="K0W65_002061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095328.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21675..21995)
                     /locus_tag="K0W65_002062"
     CDS             complement(21675..21995)
                     /locus_tag="K0W65_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095329.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22129..23562)
                     /locus_tag="K0W65_002063"
     CDS             complement(22129..23562)
                     /locus_tag="K0W65_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBI3095330.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23625..24074)
                     /locus_tag="K0W65_002064"
     CDS             complement(23625..24074)
                     /locus_tag="K0W65_002064"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBI3095331.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24071..27157)
                     /gene="ebgA"
                     /locus_tag="K0W65_002065"
     CDS             complement(24071..27157)
                     /gene="ebgA"
                     /locus_tag="K0W65_002065"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HBI3095332.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27340..28323)
                     /gene="ebgR"
                     /locus_tag="K0W65_002066"
     CDS             complement(27340..28323)
                     /gene="ebgR"
                     /locus_tag="K0W65_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBI3095333.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28541..28872
                     /locus_tag="K0W65_002067"
                     /pseudo
     CDS             28541..28872
                     /locus_tag="K0W65_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28914..30293)
                     /gene="ygjG"
                     /locus_tag="K0W65_002068"
     CDS             complement(28914..30293)
                     /gene="ygjG"
                     /locus_tag="K0W65_002068"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBI3095334.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30711..32231
                     /gene="aer"
                     /locus_tag="K0W65_002069"
                     /pseudo
     CDS             30711..32231
                     /gene="aer"
                     /locus_tag="K0W65_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32282..32905)
                     /gene="nfeR"
                     /locus_tag="K0W65_002070"
                     /gene_synonym="yqjI"
     CDS             complement(32282..32905)
                     /gene="nfeR"
                     /locus_tag="K0W65_002070"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HBI3095335.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33182..33927
                     /gene="nfeF"
                     /locus_tag="K0W65_002071"
                     /pseudo
     CDS             33182..33927
                     /gene="nfeF"
                     /locus_tag="K0W65_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(33981..34056)
                     /locus_tag="K0W65_002072"
     tRNA            complement(33981..34056)
                     /locus_tag="K0W65_002072"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34021..34023),aa:Ile,seq:cat)
     gene            34181..34687
                     /gene="mug"
                     /locus_tag="K0W65_002073"
                     /pseudo
     CDS             34181..34687
                     /gene="mug"
                     /locus_tag="K0W65_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8468 a         8920 c         9102 g         8197 t
ORIGIN      
        1 aatttcaacg gttatgatgc agatgatgtc agtgacagta atactgacgt cagcgatctc
       61 tattatcgtt atgatttttt acgtcgttat tatgtgcagg cgggtcgcat ggacaaccgc
      121 acactattta atgcacaagg cgggaacttt acctttaact ttctgccact cggtgcaatc
      181 gatgggatgc gtatcgggtc gacactgagc tatttaaacc aggcgcaaag ccagcaagga
      241 accccagtaa tggttctgct ttcgcgcaat tctcgtgttg acgcttatcg taatgagcaa
      301 cttttgggat cgttttatct caatagtggt tcgcaattta ttgataccag ttcctttccg
      361 ccaggcagct acagcgtggc attaaaagtc tacgaaaata accaactcac ccgcaccgag
      421 cttgtgccgt ttaccaaaac cggcggtctg actgacggaa atgcgcaatg gttcttacag
      481 gcaggtaaaa ctacatcaca ggtttctgat gatgaaagtt cagcttatca gctaggagta
      541 cgcctgccat tacatccgca atatgagctc tacgcagggc tggcgaatgc tgatgatgtg
      601 agtgctttcg agttaggtaa taactggacg gcagatttag gcagggtggg gaatcttgca
      661 atcagcgcca gcgtgttccg taacgatgac ggcggcaaag gtgatatgca acaggccaac
      721 tggagtaatc cgggatggcc gacgttgggc ttttatcgga ccaactctga cggcgatgct
      781 tgtacaaccg acagcagaga gagctataac gccttaagct gttatgaaag tatttccgcg
      841 acagtttcac tgaattttgt cggctggaat atgatgctgg gttatacctg cacacaaaat
      901 aacactgatg atagtttgcg ttgggataaa cagcagagct ttgaaaataa ctatcttcgc
      961 cagacaacag cgcaaagtat ctctgaaact gtacaactta gcgcttcccg cgctattgtg
     1021 atgcgtgact ggattttgag tacgtcagtc ggtgttttcc atcgtaatga caacggtggc
     1081 gataacgacg acaacggctt gtacttatcg ttttcgttat ctgacacgcc aacgatggat
     1141 agcaataaca acagccattc aaccaatgtg cctacggatt atcgttatag cgaacaggat
     1201 ggcgatcaaa cgtcatggca gttatcgcat accttttata acgattcatt cagccataaa
     1261 gaacttggcg taaccgtcgg aggcctgaac accgatacca taaacagcgc ggttaacggg
     1321 cgttgggatg ggcaatacgg aaatgtctac gctaccgtat ctgatagtta tgaccgtaag
     1381 aatcatgatc atctttcggc ctttacgggc acttatagct ctacgctggc tgtcagtcgc
     1441 tatggcgtta atttgggagc cagtggtaca gacgatttgc tgggtgcggt actggtggat
     1501 gtgaaaggct tctctgaaca ggatgaagag agtcaggatc tgcaactcga agcgcgggtg
     1561 gcaggcagcc gaactttgca gcttggtcaa agtgacagtg tgttgttccc ttatcctgga
     1621 tttcagtctg gttttgttga ggttaacgac agtagccagg gcaatcagca ggggacaaca
     1681 aacatcatta acggtgcagg gaatcgtgaa ttaatgttgt tgcctggcaa gctgcgctat
     1741 cgcgaagtgt ctgccagctt taactacaac tatatcggtc gtttattact gccggcagcg
     1801 gtgaaaaaat tcccgattgt ggggctgaac agcgccatgt tactggtagc tgaagatggc
     1861 ggatttacac ttgaaattaa cggcagcgaa aaagagctct atctgctttc cgggcagcaa
     1921 ttccttaagt gtccactgag tgttgtaaag aaacgcgcca gcattcgtta cagcggagat
     1981 gttacttgta gtgtggtgac ttattcacaa ttaccggagt cgattcaggt tcaggcacag
     2041 ttgaaacagc ctaaattacg tggaaacgtt cagacggcgc aaagggaggt tgcaccatga
     2101 gaaatcgatt gattgcggcg atattgggct tgtttggcac gctcactggc gttcaggcag
     2161 ctcctgacgt gaccagtgaa attacgtatg atttggcatc tggtagagcg gattattact
     2221 tctggaagga tgaagcgtcg gcaggaaata atggatatat gtggtatgaa tgttcgtatc
     2281 ctgacctcca acaaacctgt acagctaatg gaaatatatc gacagtacaa atctatttaa
     2341 ctgaacaacg cagtgggatg cgttggccgg taaaactcaa aggatttaaa acagccattg
     2401 taagtagcga tgaagcgccg ctaggatgca aggggggcaa agggcttcag acgaatctta
     2461 aggattctaa tagatcttca tgtacagaag atggtcaaca ttattatata tacgatacaa
     2521 agtttcttac gctctacctt gagcagacag agatgaagaa tttgccgatt ggtggcgtct
     2581 ggaaggggaa agttaaatta cattcgaaca gcccggccca ggactatttc gcaaatatta
     2641 ccctgaatac gctcgacccc aaccatattg acgtgttctt cccggagttc gcccacgcca
     2701 cgccaagggt gcagttagac ttgcatccaa caggaagcgt taacggcagc aactacgcgc
     2761 aagatctgac catgttggac atgtgcctgt acgatggttt taacggtaat gccatcagtt
     2821 atgaaatcat gctcaaagat gaagggcgac cagctgcagg gcgcagagac ggttacttct
     2881 ctatctatcg tcagggaggg accaccaccg acgagggaga acgcattgat taccgggtca
     2941 aaatgtacaa cccggaaacc ggtgggcaaa ttgatgtgcg caataatgaa aatatggtct
     3001 ggaacagcat taacctgaaa cgtgtgcgtc cggtggtact gcccggtatt cgctatgccg
     3061 tgatgtgtgt gccgacaccc ttgactctgg cagtcgataa attcagcgtg atggataaac
     3121 aggccgggta ctacatgggc aaattgtcag taatctttac gccttccttg ccaaccatca
     3181 attaaccctg caaaccggac aaacgctttt ttctatataa acgacgaagc cgcctgataa
     3241 ggcggcttcg tcgttataac ttacgttaaa cgttaatgtt gcggtgcggg atcgttatgt
     3301 gtttcctgcg ccacctcaat cgccggtttg ttctgcaata cggtccagat aaccagcgca
     3361 cccagcaggt caaatactgc cagaactgcg aacagcgggc tgaagccgat ggtgtcagcc
     3421 agtgcaccga caaccagcgc aaacagcgta cttgccagcc atgcggacat cccggttaaa
     3481 ccgttcgccg tcgccacttc gttacgaccg aacacgtcag aagagagcgt aatcagcgcg
     3541 ccagacagtg cctggtgggc aaaaccaccg atacacagca gcataattgc gacatacggg
     3601 ttggtgaaca gaccgatcat acccgggcca atcatcagca cagcacccag cgttacgacc
     3661 atcttacggg aaacgatcag gttcacacca aaccaacgct ggaacagcgg cggcaggtaa
     3721 ccaccgagga tacaaccgag gtcagcaaac agcatcggca tccaggcgaa catcgcgatc
     3781 tctttcaggt taaagccgta aactttaaac atgaacagcg ggatccacgc gttaaaagta
     3841 ccccaggccg gttctgcaag gaaacgcggc agtgcgatac cccagaactg acggttacgc
     3901 aggatctgac caacggacat tttcttcgcc gtgctcacct ggtgctgggc ttcctgacca
     3961 ttaataatat agtcgcgttc ttcatcggtc agatgcttct ggtcgcgcgg atgtttatag
     4021 aaaatcagcc atgccatcgc ccagataaag ctcaatgcac cggagatgat aaatgccatc
     4081 tgccagctgt gcatcacgat tgcccatacc accagcggcg gcgcaatcat cgcaccaatc
     4141 gaagaaccta cgttaaagta gcctactgcg atggaacgct ctttcgccgg gaaccattcg
     4201 gagctggctt tcagacccgc cgggatcatc gctgcttccg cggcaccgac cgcaccacga
     4261 gcaacagcta ggccacccca gctacctgcc agcgcggttg caccacagaa cacggcccac
     4321 agcacagcaa acattgcata accgattttc gtacccagca catccagtac ataacctgct
     4381 accggttgca tgaccgtata agcagcagaa taggctgcga tgatatagga atactgttgg
     4441 gtggagatgt ttaactcttc catcagagtt ggcgcagctg ccgccacagt gttacgcgtc
     4501 aggtaaccaa gcacggtgcc gagcgtcacc agtgcgatca tataccaacg taacccttta
     4561 attttacgca tgtaaaacct catcgttttg ttatatccgc aggaagcgcg gccatcatcc
     4621 cgccagaggc tgaagatgtt agttacgacc gggtaaccga aaaaaaaaac cggcacgccc
     4681 cgaacgttgc catgaaagtt tcgtgctaat tcggcttccg taccggttaa cccgattcat
     4741 ctaacaaatg aatgaatcgg caatttgcat tccgtcgact tatgatttgc gacggcagaa
     4801 agataacttg tcatacaacc ttaaaaggtg agagccatca caaatgtgcg aatatttgta
     4861 gggacattac ctgacgacag cgaggccagt actggcgcgg cctgcagcga gatttaccac
     4921 tttgagagta atttttttaa ctacgtttat tgatctaact cacgaaaata tcttcggact
     4981 ctggaaattg gtgtgataac tttgtcagca tcgcaccata agcaagctag ctcactcgtt
     5041 gagaggaaga cgaaaatgac tccgtttatg actgaagatt tcctgttaga taccgaattt
     5101 gcccgccgtc tgtatcacga ctacgcaaaa gaccagccga ttttcgatta ccattgccat
     5161 ttgccgccgc agcagattgc ggaagactat cgttttaaaa acctgtatga catctggttg
     5221 aagggcgatc actacaaatg gcgcgctatg cgtaccaacg gtgtggctga gcgtctgtgt
     5281 actggtgatg cgtctgaccg tgaaaaattt gacgcctggg cggcgactgt tccgcacact
     5341 atcggcaacc cgttatacca ctggacgcac ctcgaactgc gtcgtccgtt cggtatcact
     5401 ggcaaattgc tttctccgtc aactgccgat gaaatctgga acgaatgtaa cgaactgctg
     5461 gcgcaggata acttctccgc gcgcggcatc atgcagcaga tgaacgtgaa aatggtcggc
     5521 accaccgatg acccgatcga ttctctggag catcacgcag agatcgccaa agacggctct
     5581 ttcaccatca aagtgctgcc gagctggcgt ccggacaaag ccttcaatat cgaacaggcg
     5641 acctttaacg actacatggc gaagctgggc gaagtttccg ataccgacat tcgccgcttt
     5701 gctgacctgc aaactgcctt gaccaaacgt ctggatcact tcgccgctca cggctgtaaa
     5761 gtgtctgacc atgcgctgga cgtggtgatg tttgctgaag cgaacgaagc ggaactggac
     5821 agcattctgg cgcgccgtct ggctggcgaa accctgagcg agcacgaagt ggcacagttc
     5881 aaaactgcgg tactggtgtt ccttggtgcc gaatatgcac gtcgcggctg ggtacagcag
     5941 taccacattg gcgcgctgcg taataacaac ctgcgtcagt tcaaactgct ggggccggat
     6001 gtaggctttg actccatcaa cgaccgtccg atggcggaag agttgtctaa gctgctgagc
     6061 aagcagaacg aagaaaacct gctgccgaaa accattctct actgcctgaa cccgcgcgat
     6121 aacgaagtgc tgggcaccat gatcggtaac ttccagggcg aaggtatgcc gggcaaaatg
     6181 cagttcggtt ccggctggtg gtttaacgat cagaaagacg gtatggaacg tcagatgacc
     6241 caactggcgc agctcggtct gctgagccgc tttgtcggta tgctgactga cagccgtagc
     6301 ttcctgtcat acacccgtca cgaatacttc cgccgcattc tgtgccagat gatcggtcgc
     6361 tgggtggaag cgggcgaagc accggcggac atcaacctgc tgggcgagat ggtgaaaaat
     6421 atttgcttta acaatgcgcg tgactacttc gccattgaac tgaactaagg tctggggttg
     6481 atatgcaata catcaagatc catgcgctgg ataacgtcgc ggtcgcttta gcagatttgg
     6541 ctgaaggcac agaagtcagt gtcgataacc agactgttac gctgcgccag gatgttgctc
     6601 gtggacataa atttgcgtta acggatatcg caaaaggggc caacgtcatc aaatatggcc
     6661 tgccgattgg ttatgcattg gcggatattg cggcggggga acacgtccac gcccacaata
     6721 cgcgcacgaa tctgagcgat ctggatcagt atcgctatca acttgatttt caggatctgc
     6781 ctgcgcaagc ggcagatcct gaagtgcaga tctatcgtcg cgctaacggc gatgtcgggg
     6841 tgcgtaacga gctgtggatc ctgccaaccg tgggctgtgt caacggcatc gcgcggcaaa
     6901 tccagaaccg tttcctgaaa gagaccaaca acgccgaagg taccgacggc gtgttcctct
     6961 tcagccacac ctatggctgc tcacaactgg gcgacgatca catcaacacc cgcaccatgc
     7021 tgcaaaacat ggtgcgccac ccgaacgcgg gcgcagtgct ggtgattggt ctgggctgtg
     7081 aaaacaacca ggttgccgca ttccgtgaaa cgttgggcga tatcgatcct gaacgcgttc
     7141 atttcatgat ctgccaacag caggatgatg agatcgaagc cgggatcgag catttgcatc
     7201 agctgtataa cgtgatgcgc aacgataaac gcgagccagg caaactcagc gaactgaagt
     7261 ttggtctgga gtgcggtggt tctgacggtc tttctggtat tactgctaac ccgatgctgg
     7321 ggcgtttctc tgactacgtg attgctaacg gcggtactac cgtactgacc gaagtgccgg
     7381 agatgtttgg cgcagagcag ttgctgatgg accattgccg cgacgaagca acgtttgaaa
     7441 aactggtcac catggtcaac gacttcaaac agtactttat tgcccatgat cagccgatct
     7501 acgaaaaccc atcaccgggg aacaaagcgg gcggtatcac cacgctggaa gacaaatcac
     7561 ttggctgtac ccagaaagcg ggttccagtg tcgtggttga cgtgctgcgt tacggcgagc
     7621 gtctgaaaac gccagggctg aacttgttaa gtgcgccggg taacgatgcc gtagcgacca
     7681 gcgccctggc gggtgcgggc tgccatatgg tgctgttcag tactggtcgc ggcacgccat
     7741 acggtggatt tgtgccgacg gtgaaaatcg ccaccaacag tgaactggcg gcgaagaaaa
     7801 aacactggat cgactttgac gcgggtcagc tgatccacgg taaagcgatg ccgcagttgc
     7861 tggaagaatt tatcgacacc atcgttgagt ttgccaacgg taagcaaacc tgcaacgagc
     7921 gtaacgactt ccgcgaactg gcgatcttca aaagcggcgt aacgctataa agtcgaaatg
     7981 agttgttaat aaaaaacggc gtttcataat gtgaagcgcc gtttgctttt ccatacctgc
     8041 taaggattta tcatgaccgc gtattggctg gcccagggcg tgggtgtcat cgcctttctg
     8101 attggtatca caacattttt caatcgtgac gaacgtcgct tcaaaaagca gctttcggtc
     8161 tatagcgccg ttattggcgt acattttttt cttctgggca cctatcccgc tggtgccagt
     8221 gccatcctta atgccattcg tacattgatt accttacgca cgcgcagctt atgggtaatg
     8281 gcgattttta ttgtgctgac tggcggaatt ggcctcgcga agttccatca tcctgtcgaa
     8341 ctattgccgg ttatcggtac gattgtcagt acctgggcgc tgttccgctg taaagggctc
     8401 accatgcgct gcgtgatgtg gttttcaacg tgttgctggg tgattcacaa cttctgggcg
     8461 gggtcgatag gcggcacgat gattgagggt agttttctgc ttatgaatgg cctgaatatc
     8521 attcgtttct ggcggatgca gaaaagggga attgatccgt ttaaagtaga gaaaacccct
     8581 cccgccatag acgaaagggg ttaaacaatt aattacgcag ggcgctattt gccagacgat
     8641 cgtcttccgc ctggcaagct gccgcagtga acagcacgtc agttgaagag ttcagcgcgg
     8701 tttcgcaaga gtcctgcaat acgccgataa taaagccgac ggcaaccacc tgcatggcga
     8761 tatcgttcga aataccgaac atattacagg ccagtgggat cagcagcaaa gaaccccccg
     8821 ccacgccgga tgcgccacag gcacacagag aagccactac gctcaacagc agcgctgtgg
     8881 gcagatcgac cggaataccc agcgtattaa ccgcagccag cgtcaacacg gtaatggtga
     8941 ttgctgcgcc cgccatattg atggtggctc ccagcggaat agaaactgaa taggtatcgc
     9001 gatccagatt cagcttttca cacagcgcca tattcaccgg aatgttcgct gcagagctgc
     9061 gggtgaagaa ggcatacaca ccgctttcgc gcaggcacag cagcaccagc gggaacgggt
     9121 tacgacgaat tttccaccac accagcaatg ggttaaccac cagcgccacc agtaacatac
     9181 agccaaccag cacgaccagc agttgcgcgt agccccacag tgtggagaaa ccggtggttg
     9241 ccagggtaga agaaaccagc ccaaaaatac cgaacggtgc gaagcgaatg accagtttca
     9301 ccataaaggt aacggcattc gacatatcat tgaccaggtt tttggtggtc tcgttaccgt
     9361 gacgcagtgc gaagccgagg ccgatcgccc acaccagaat cccgatgtag ttacctttca
     9421 gcagggcgtc gatggggttg gaaaccatgc tcattaccag tccgcgcatc acttcgacaa
     9481 tgcctgacgg cggcgaaata tcacccgcgc tactggacaa gtgcagggta gaagggaagg
     9541 caaagctgaa gactactgcg gccagagctg cagagaaggt gcccagcaga tagaggaaca
     9601 aaataggacg gatattggtt ttctgcccgt gctggtggtt agcaatagat gccattacca
     9661 gcatcaacac caggatgggg gcaacagctt tcagtgcgcc gacgaacaaa gtacctaaca
     9721 gaccaacagc ttccgccgcg ggttttgaga tccatgccag aagaatcccc agaacaaggc
     9781 cgaccaggat ttgttttacc aggctgccat gagccagacg ccggaatagc cccggtgaac
     9841 gttgcgtagt catttttcga tcctttcatt gtgttgtcgc acatccctgg tgcgttctgt
     9901 cgacgcatct ttcaggatgt aacaaagtgt ttgcaaggtc gagtataagg aaagttacag
     9961 cttcgggaag cgaaaaatgc tggattttaa agagaggtca tgttttgcaa cttaatggtt
    10021 gcatttattt aacataaaca gagggaagga ctggctacta aggctgatta gataacatat
    10081 gggcaaaact atttggaata tgagaacggt tgcgctcctt taacgtgtta tgccatataa
    10141 agtcactggc gcagggagcg cgcagggggc ggccaatcgc cgccgccccc tgcaccccca
    10201 ggctctggcg aacaaaatcg ccgctgcgcg gttccctcag cctatccctt gcggctaccg
    10261 ggtcgggcgc aaggtaacgt ccctgtaaga tgcgccctcg ccccggcctc cagggaacgc
    10321 ttcggcgatt ttgacgccac caacacccgt gcggtctttt tgtaaagagc tataagccga
    10381 ttacataatt aatagctcac ctttgtctgg aatgagttat ctctaggaat ggcttaatat
    10441 acagcgaatc attctccccg ctgcttatca tgccgataat tcacccaggc gttgataata
    10501 aacgtcatca ccagaatgcc aaacaccacg cccagcgaga cggcgattgg aatatggtag
    10561 aagtcgacaa tcagcatctt gataccgata aacaccagaa tcaccgccag gccatatttg
    10621 agcatcgaga aacgctctgc tacgcccgcc agcaggaaat acatcgcacg caggccgagg
    10681 attgcaaaca ggtttgaggt cagcacaatg aacgggtcag tggtcacggc gaagatagcc
    10741 ggaatgctat ccacggcaaa aatcacgtcg ctcaattcca ccagaatcag taccagcatc
    10801 agcggtgtgg cgtacagcaa tccattctta cgcacaaaga aatgctcgtt gtcgatggtg
    10861 tcggtcatgc gcaaatgacc gcgtagccag cgcaccagcg gcttgtcgcc aatgcctgat
    10921 tcatcttcat gggcgagcgc catcttcacg ccggtaaaca gcaggaaggc accgaagata
    10981 tacaggatcc agtcgaactg cgaaatcagc cagctgcctg tgaagatcat gatggtacgc
    11041 agaacaatcg ccccgagcac gccatacacc agcacgcgac gttgtaatgc cgccggaacg
    11101 gagaaatagc tgaacaacat cagccagaca aagacgttat cgaccgccag cgatttctca
    11161 atcagataac ccgtgagaaa ggccagggcc tgtggatcgg caacggcgcg accttcggtt
    11221 tgcaccagat accaccagaa agcggcgtta aacagtaacg acagcgtcac ccagaccagc
    11281 gaccaggccg cagcctgttt catggtcatg gcatgtgccc cacgacgccc ctgcaacaac
    11341 aggtcgatag ccagcataat ggcgacaaca acagcgaatc cgccccatag caacggcgtg
    11401 ccgacagtat tcatagaagt tccttacaca taaaaaaacg gccaacgtcg gaagacgtca
    11461 gccgctgctt tttatgcata gacctcgcct tccggcaagg tctcacttac aacaaaaaag
    11521 gaatacgtct gcgtattcct ttcgggttgc ccggtgaccg gatgtggttt ttcactcatc
    11581 gtaatgacga tcgaccggca atgaagttac tcccctttgc aggtaacaaa gtatgacaga
    11641 ccattcagtc agtcaatggt ctgttacata ccttttacac tgttttactt tggcaagtat
    11701 agttttacgc tatctgccgg aaaaatcacc ccagtttggc ggcggatctc ggtcagcagt
    11761 ttggctgtga tgcgactgac cgccagccct ggatgatcca ccagatgctc atccaccagg
    11821 gtagcgaaca gctctgcttc gtagagcatg gtattaatat gctgcggctg ggtgagatcc
    11881 tgcatctggc tgccacgcgg cacaaagcag actttctggc attcagacag tttttcaatc
    11941 accagcgatc ccgcttcgcc ctgaatctcg cttcccagga cagaatcact gacctttgag
    12001 tgctgcaagg tgacgctgaa atcaccgtaa tccatcacca ccacgccgtg ggcgtcaacg
    12061 ccgcttgcca gcaaactcgc cgttgcctgc acgcttttcg gctcaccaaa taacgccacc
    12121 gccgacgcca ggcagtaaaa gccgatatcc ataattgaac cgttagagaa tgccggatta
    12181 aaggtgttgg gattctcacc gtccaggtaa cgttgatacc gcgaggaata ctggcaatag
    12241 ttgaaaaaga ctttacgcag tttgcctgct ttcggcagcg cctggcgcaa caaatgaaag
    12301 ttcggcaggc aggcggtttt aaatgcctca aacagcacca cctgattttc gcgcgcacag
    12361 gcaatggcgg catccacttc cgccagattc gacgccagcg gtttttcgca aatcacatga
    12421 attttatggc taaggaaaag ttgtgtctgg gaaaagtgca gggaattcgg gctggcaata
    12481 tacaccgcgt caatggcatc gctttccgcc atcgcttcca gcgacgtaaa cagatgctcg
    12541 acagaaaaat cattggcgaa gtgctgagcc tgctcaaggc tgcgggaata tacggcggtt
    12601 aacttgtatt taccgctctc atgggcggcc tcgacgaact ggcgagtgat ccagttcgta
    12661 ccaatcacag cgaaacgtat cataacgcgt ggatactcca taaagggatt ctgtcgtcaa
    12721 tgtagcacgc ctttttcatc ttcgacgtgc gcagatacgc attattcttt cgccggacag
    12781 ccatgctcac taaacggccc aatcatgacc gatggcccga gatatttcgg ctggcgatgg
    12841 aggatttttg cgtcaatcac cgcgctgaca cgagcgagtt tctcccgcag ttgagtataa
    12901 aaaccgtgct tcatattgag gtctggcgcg ttaaacccga tagcgtcgat gccgtactgt
    12961 tttgccagcc agatggtgcg ttggttatga aattcttgcg agataatggt gatatggttt
    13021 tccccaaaca cctttttggc acgcactacc gaatccagcg ttgagaatcc ggcatagtcg
    13081 caaaagatca ctttcgccgg tacgccttta gcgatcaaag cctgctgcat acctgatgct
    13141 tcatcgtaat ttttacgtcc gttatcgcca ctcaccagta gccatttcac ttttccggcg
    13201 tggtacaacg ctgctgcggt atcaatacgt cgggtaaagt agcgattacc cggcctcgcc
    13261 cccagcaata aacccacgtt acgcgccggg acggcgttga catcgcccca ggtcagctgc
    13321 ttgctggcgt tgaccataac gcgatcggcg ataaaaatgg tcgctcctgc cacaagtaac
    13381 agtaagcagg caatctttag agtccggcga gaaaaacaaa tacggagaag aaggcgggca
    13441 aatgcgcgca gcatggtcgt tatccttaac gaaagggggg aggtcgattt taggcgctaa
    13501 agaaagaaaa gataacgcct gacatcattt tcagattttg tcctgcccaa gcgacaactg
    13561 cgttagccac agccgggtgt cgaactcaag ctggtggtac tgcggctcca tatggcagca
    13621 caattgatag aacgctttgt tatgctcttt ctccttcagg tgcgccagct cgtgcaccac
    13681 gatcatgcgc aaaaacggtt ccggcgcgtt gcgaaacacg gtagcgacgc ggatctctgc
    13741 ttttgctttc agcttgctgc cctgcacacg agaaacagcg gtatgtaacc cgagcgcgtt
    13801 ttttagcaca tggatcttat tgtcatacat cactttattg atcggcgggg cattacgcag
    13861 aaactgattt ttcagatcct gagtatattg ccagagagct ttatcggtgg cgtagtcgtg
    13921 tgttcccgga tagcgttttg ccagcacatc gcccagacgc tgttcgttaa tcagtgttcg
    13981 cacctgcgaa agcagctgct cgggataacc ctgaagataa gtaagattgc tcataaccgc
    14041 ccacgaaaaa gagaaaaagg gtatactcac gcaccctttt caggggcaac gtcgaaattt
    14101 tatcattcag gagggccgat gagccactta gacaacggtt tccgttcact gacactacaa
    14161 cgttttccgg cgacggatga cgttaacccg ctacaggcgt gggaagcggc agatgaatat
    14221 ttgctgcaac agttggacga cacagaaatc ctcggcccgg tgttgatcct gaatgatgcc
    14281 tttggtgcgt taagctgcgc gctggcggaa cataagccgt acagcattgg cgactcatac
    14341 atcagtgaac tggcgacgcg cgagaattta cgcctaaacg ggattgatga gtcgagcgtg
    14401 aagtttctcg acagcaccgc cgactacccg caacagccgg gtgtggtgct gatcaaagtg
    14461 ccgaaaacac tggcattgct ggaacagcaa ctgcgtgcgc tgcgcaaagt ggtcacgtcg
    14521 gatacacgta ttattgccgg tgccaaagcc cgtgacattc acacttccac gctggaactg
    14581 ttcgaaaaag tgctcggccc gaccaccacc acgctggcat ggaaaaaagc gcgcctgatt
    14641 aattgcacgt tcaatgaacc gccgctggcc gatgcgccgc agaccgttag ctggaagctg
    14701 gaaggtactg actggactat ccataaccat gcgaatgtct tctcccgcac cgggcttgat
    14761 atcggcgcgc gcttctttat gcaacatctg ccagagaatc tcgaagggga gattgtcgat
    14821 ctcggttgcg gtaatggcgt tattggtctg acgctgcttg ataaaaaccc gcaggcgaaa
    14881 gtggtgtttg tcgatgaatc gccgatggcg gttgcttcca gccgtttgaa cgttgaaacc
    14941 aacatgccag aggcgttgga tcgcagcgag tttatgatca acaacgcgct ctccggcgtg
    15001 gagcctttcc gctttaatgc tgtgctctgc aaccctccgt ttcaccaaca acatgcgctg
    15061 accgataacg tcgcctggga gatgttccac tacgcccgcc gctgcctgaa aatcaacggc
    15121 gagctgtata tcgttgccaa ccgccatctg gattacttcc ataaactgaa gaaaattttc
    15181 ggcaactgca ccactattgc gacgaataat aaatttgtgg tgctgaaagc ggtgaagctg
    15241 ggacgtcgtc ggtaagattg ggagttacct gccggatgcg gcgtaaacgc cttatccggc
    15301 ctacgttcag cacgttaccc ccaggccgga taagacgcgg caagcgtcgc atccggcaat
    15361 gtctgctcag ttctggaatc gcgcttccat aaaacaaaaa atccctgtca ccatcctcta
    15421 cattctcttg tttagcgttt ttctacgttt attcttccgt cacacagata aattcatccg
    15481 ttgccaatct gtcaacggat gttatcatat tgccaatttg tcaacggacg tgatgaagaa
    15541 tgcacctgat aactcaaaaa gcattgaaag atgctgcgga aaaatacccg caacataaaa
    15601 cggagttggt ggctctgggg aacacgattg ctaagggata tttcaaaaaa cctgagtcat
    15661 taaaagcagt attcccatct ctggataact tcaaatatct ggataagcat tatgttttca
    15721 atgttggggg caatgaatta cgtgttgtag caatggtctt ttttgaatcg caaaagtgct
    15781 acatacgtga agttatgacg cataaagaat acgatttctt taccgctgtt catcgtacta
    15841 aggggaaaaa atgattgcga ttgccgacat cttgcaagca ggagaaaagc taactgctgt
    15901 ggcacctttt ctggcgggta ttcagaacga ggaacaatac acccaggcgc tggaactggt
    15961 agatcatctg ctgctcaacg atcctgaaaa ccccttgctg gatctggtgt gtgccaaaat
    16021 aaccgcgtgg gaagaatcag cgcccgaatt tgcggaattt aatgccatgg ctcaagccat
    16081 gcctggcggt atagccgtga ttcgtaccct tatggatcaa tatggtttaa ccctttccga
    16141 tctgccggaa attggcagta aatctatggt gtcacgcgtt ttgagcggga agaggaaatt
    16201 aacgctggaa cacgctaaaa aattggcaac gcgattcggc atttctcccg ccttgtttat
    16261 tgattaacgc gacgtgccga atgcgttgca tccggcgctt caaataacga tttaaatctc
    16321 cagcgccagc cgtgttccct gggcaattgc ccgtcgtgcg tccagctcca tagccacatc
    16381 gcagccgcca attaaatgca cagttttccc gctatcaatc agcggttgcg ccagcgcgcg
    16441 gtttggctct tgccctgcgc agatcaccac attgtccact gctaatacct gcgtttcgcc
    16501 gttgatcacc acatgcagcc cgtcatcgtc aatcttctga taacttacgc ctgggatcat
    16561 tttcacaccc cgcgagagca gggtggtgcg atggatccag ccggtggttt tgcctaaccc
    16621 ctgtcccggt ttgctggctt tgcgctggag catcacaatt tgccgtgggc tacgcgggat
    16681 ctgcattccc tgcgggctta agccaccagc ctgttgtagg ctgctgtcga tcccccactc
    16741 attacagaac ctggcgatat tctggctggt ggattcgccc ggctgactta aatacatcgc
    16801 cgtatcaaaa ccaatcccgc cacaaccgat gatggcaacc ttgttgccta ccggcgcttt
    16861 gtcgcgcagt acatcgagat aactcaatac cttcggatga tcgatcccgt cgataggcgg
    16921 aacacgcggc acgatcccac tggcgaggat cgtttcatcg aacgcctgta actgatccgc
    16981 cgtcacggtg tgattgagtt tcagcgtcac gcccgtcact tcgatcatcc ggcggtagta
    17041 gcgcagcgtt tcgtaaaact cctctttgcc ggggatctgt ttggcgatat taaactgccc
    17101 gccgatctcg ctgtgcgcat caaacaatgt tacctgatgc ccacgcgccg ccgcgttaat
    17161 ggcaaacgcc agcccagcag gtccagcgcc gaccaccgcc agattttttt tctgcacggc
    17221 gggaaggatt ggcattttgg tttcgtggca ggcgcgagga ttcaccaggc acgaggtgac
    17281 tttgccaacg aagatctgat cgaggcagcc ctggttgcag ccaatacaag tgttgatctc
    17341 atcggctcgc cctgattgcg cttttgacag cagttccgca tccgcgagaa acggtcgcgc
    17401 catcgatact atatcggcat cgccgcgcga gagaatatcg tcggcaacct gcggatcgtt
    17461 aatccggttg gtggttacca gcggcagcga gacgtggcct ttcagtttgc gcgtgaccca
    17521 gctaaatgcg ccgcgcggca cgggcgtggc gatggtcgga atccgcgctt cgtgccagcc
    17581 tattccggtg ttgataatgg tcgcgcccgc cgcttcaatg gcctgcgcca gttctaccgt
    17641 ttcggcaaaa gtgccgccgc cttcgactag gtcgagcatc gacaaccggt aaataataat
    17701 gaagtcgttg ccgacgcgtt cgcgcaccgt acgcactact tctacggcaa atcgcatccg
    17761 gttgcggtaa tcgccgcccc actggtcact acgctgattg gtgcgcagcg tcagaaattc
    17821 gttgatcaaa tacccttcag agcccatcac ttcgacgcca tcgtatccgg cctcccgcgc
    17881 cagttgcgcg cagcgggcga aatcgtcgat cagttgcagg atctcttcat gggtgagttc
    17941 gtgaggaacg aaacggttga tgggggcctg caatgcggac ggtgcgacca gatgcggttg
    18001 gtagctgtag cgcccggtat gcaaaatttg cagggcgatt ttgccgcctt cctgatgtac
    18061 ggcttcggta atggtgcgat ggtgtgggat ctggctggcg tcgttgagca ttgcgccacc
    18121 ttccatgcca acgcctgtta aatctggtgc aatgccgccg ctgacaatca gcgccacgcc
    18181 gtgacgggcg cgttcggcat aaaacgcagc cagccgttca gcaccgtccg ggtattcctc
    18241 cagcccggtg tgcattgaac ccatcaacac gcggtttttt aacgtggtaa aacctaaatc
    18301 cagcggggcg aacagcgacg ggtagctcat aaaattgtcc agtatgtaaa ataattgtta
    18361 tgtagtcgga tgagttctaa tttagccgtc gatggagaaa agggaaaagg ggagtgcggg
    18421 gtttgtgatg ggattcaaaa agaatacgcc cggtggtaac gccggatgcg acgcttgccg
    18481 tgttttatcc ggcttggggg attgcacatg tagggcggat aaggcgttca cgccgcatcc
    18541 gccagccgtt gcaaattact gcttacggaa aaaatcgtta tacaacatat acaaatgagc
    18601 ggcactccag gagaaatttg gtgcgccttg ctgcgcgcct gtcagcgggt tgtaattctc
    18661 ctgaattggg ccatcggcgg ttaatccttt ggcgtgcctg aagaacgtat ccgccagctt
    18721 cagggcatca tcacgataac cgtaacgctc catccctttc agaccaaacc agaactgatc
    18781 cacccatacg cgcccgcgcc agtagatatc agcgccaaaa gccggattgg ttaacgccgc
    18841 cgttcccagc ggaacaaatg tgttgaactc tttaggatcg agcatcacct tcaccaccgc
    18901 gtcggcattg gcctgcgttg ccgcaccgtt aaacagcggc gaccagcctt ccggcccttt
    18961 accgcgctca acaatcggtt tgcccgcgca gccgttcgcc agtggtttat cttcaatacg
    19021 tacgtcatag tagaactggg tagtcgggtc gaacatacag gtgttgatgt agtccgcgag
    19081 ctgctgtgcc aactggcgat agcgtttggc ctcttctggc ttaccgagga tggttgccat
    19141 ctccgccaga taatggttat cgctgtacat atagctggcc tgatccaccg actcctgcaa
    19201 tagcgagtag cccagcaacg ttccgtcctg actgcggttt tcggcgaatt tcaccgtcca
    19261 gtcgctacgt ttgccgccat tagcgacata tttatccaac tgttctttgt cgataaaacc
    19321 aaagacggcg gcatcatcac gccccgattc ccacgacgca gcgacctgtg ccggaatttc
    19381 cagactgtcg tactggcctt tctccaccac gcgggcgtag ttgttcagcc cagactgcgt
    19441 ctcttcttta tcgccttttt tcaccgtaaa cagcatctcg ccgctctcgg tgttgtgggc
    19501 tttgtcgcgg gtcgcgccat attccggcac cccgttgccg ttatgatcgc ggttacgtaa
    19561 ccaccagtcg tgataggcca ccagtttcgg gtacatctct gccaaccagg ctttatcctg
    19621 ggtgacgttg tatacttcca tcaccgacca ggcggcaagg ctgggtttgg tattacgttc
    19681 gttccagttg ccgccatcgc caccacgctc ggggctaagg ttccacgcta tcaggtcggg
    19741 gacaaagccc acatcctgcg gacgcacgct gtcgccaggc tggatctgcc aggagaagac
    19801 cgcgcggata ttctctttgg cgatatccgg attgaaatgc gccatcgcaa acgcctgctt
    19861 ccaggtatcc cacggccagg tctgattgcc ggagaaccag cgtccggtca ccgacggcgt
    19921 gacggtgtta taattcaccg caccgcccgg tgagcgccag ttaccgttga gcgtttcgat
    19981 cgctttcacc gcgacgcgcg tctgttccgg cgtcgcatcc ggattggtta accctttctt
    20041 caggtattct tcccagcgtt gctgcgaggc ggtgagataa aacgccggac gcgccagaat
    20101 atcgcggatc tgcatttgct ctttgctaac ttcctgagcg gtcagcagat gggaataggt
    20161 ggtgtagagc gtggtcgaac cgttgatatg cgccttactg gtaaagcgat taccgttgat
    20221 ttcagtctgc accggcaggg atttatgcac ctgatattcc gattcgccgg aggtcagcag
    20281 atcccaggtg gcgcgcactt tgccaaaggt gactttcagg ccatcacggg tggcgctgat
    20341 tttgcgctga tagtcagggt attcgccagc aatggtttta tcggaaagcg gtttcccttc
    20401 tttcgcttcc agtttttcca gcagttcgcc atcccacacc agatccagcg gtttattgct
    20461 gatgatttta gtttccagta gtgacgtgcg cggcgtggcg aagcgcagag tcatttcgac
    20521 ctgcacatct tttgctgtca gtttttgcac cagcgcgccg ggaatactgt atgcctccag
    20581 cgtgaagttg actttcttgc cgtcttgcca gacagtcagg cggtcgaaat tgctggccat
    20641 aaagttgatg tattcttccg tcagcagcgc aacgcccgga aagccgccca tggtgttcgg
    20701 gccgtctggc aacagatgac catgccaggc tccgagatcg aaaaacggat taaaacgctg
    20761 gtgatcgtcg taatcgtaat ccttcatgta ctgcggcgca ccagtacggt taatcacgtt
    20821 tttataattg tcggcgttag cggcatgagc ggaaaaactt atcagcagag cgcaggttac
    20881 tggcgttaaa atagttttga ttttcatgag attacagtcc ttattaccag tagaaatatt
    20941 gcatcaggaa gatttggtcg ctgggatcgt tagaggcatt tttcatgtag aagcggctat
    21001 caaaagccgt ggcgaaacgt ccgccgccat attcgtacca gataccaaac tgcatgaaag
    21061 aggtggtttc atcgtcagcg tggtcagggc gatgtttggc gtaggtgtaa gcggtggaga
    21121 gataaacgcc ttttaacgct tcatacatgg cgctcaccat catcccgctt tcacttcccg
    21181 gtacgtccag cccgtcggca cgtccggtat ggtgccaggc gcgagcggca aagttagggg
    21241 taaacaggcc cgtgagatag agttgtcctg tgccgtcggt aatttccaga ccattcatcc
    21301 aggtgatgcc ttcggcaagg tcatactgga tgtaaccgtt catccattgc cggataggtg
    21361 tatttgtcgt cgtagcgatc gtacttgcca aaatgtagcc aggctttgct ttcgtcatga
    21421 tgtccggcag gtgtggcggt gacgctataa cgaaagttgc cgcttaagtt ctgtaccttc
    21481 agcgcggtca taatgtcgcg ggtattggga atgacgtagc ccaaatcggg tgtgaagtcg
    21541 ccccaccatt gcaggtcatc cagcgaggaa tcctgacgca gactgagcat gatttcggtg
    21601 ccatcattgg tacgataacc gccatagaaa cggttaatcc caccttcgaa accaccccag
    21661 ctatggtcag gcacctaggc attaccgtcg tggtcggcct gcacggtcca gccttcgccg
    21721 taaatcaggc cgaaccagga gccgtacttc atctccaggc caccttcaat ataggtgccg
    21781 tcgctgtatt tatgtttatt ctcaccctct aaatccatat atccaccaag ccctaattcg
    21841 ccgtaaaaac gaaatgaagg agaagatgtc gaggtaattg cgggtgtttt tgtgtcgtca
    21901 tgttctgccg gacgtgcttc ttctcctgcc gcagaaaagg cgtagcaaaa cggcagagca
    21961 agtaatgctc tgcatggtgc agtgattaat ttcattgtgt taatccatga gctaaagaaa
    22021 aagtacagcc ttttatcgtt gtaattataa aaatataaag gcagaaacta ttttccctct
    22081 cctcatccag gaagagggaa aatgcctgct cattttttat tagtctgctt aatcaacatt
    22141 gctggctggc gtcgcttcaa tatggcgctc ttcagccgtt aatcccttta tatatttgct
    22201 gtatttccac cacgcaaagc cgaggaagat aacaataccg ccgacgttat aaaaaatgat
    22261 ggtcagaata ttcgcgccag tcgggaatgt cgaagcgaca aaccctacgg caaatatcgc
    22321 aatcagcatt gaaacaacga taataccggt gcggcgggag cccatacgga aatcgcgtgg
    22381 caggtgatct aatttggcgc gtaaattcag gtaagccagc atgatgaata acggcggaag
    22441 catggacgct gcggcggtca tattaataat ggtattcatc agatcctgca cggtattgga
    22501 accgagcatc gggataatca tcagcgggat gacgatcaga aactggatcc atgctgcgcg
    22561 cgccggaacg ccgttttcgt taagttcgac ggttttctta ccaaagatgc cttccgggat
    22621 ttcggagaag aaaattttca ccggcgttgc ggtccacatc agcagggaac cgaacattgc
    22681 ggtaaaggac accagaccga caaagcgatt catcaacgct tccggcagac caaaatacgc
    22741 cgccatgccg tggaatacct gcaccgatcc gccggtaaat ttcaactctt tgctgctgac
    22801 gaagacgtta atcagcaccg aggagacgga atacagtacg ccgataaaaa tcccggcgag
    22861 gatgatcact ttaacgaacg atttcgaacc gcctttgacg tcgttaacgt acaccgcgac
    22921 ggactccgcg ccacctgcgg ccataaagat ccaggtggta acgccgagga acgcccagtt
    22981 gaagttcggg atcatcgcgt caacggtgat cggatcggca ggctgtacgc cgccaaccag
    23041 tgccgtacct gccagtaaaa tgtaggagag cgttaacagc agcatcagcg ttgaagtgac
    23101 ggaggtaatt ggccccagca ttttcgcccc gttggtggaa acccaggtgg agaaggcgaa
    23161 cagcaccata ctgataacgg tggtggcaac cggcgtcata atatattcgt agccgaggaa
    23221 ggcatacgaa gcataagcaa taacgcgcgg caacagcgag gtgaaaaaga acagatttac
    23281 gaaccagtaa gtgtaggcag taataaatgc ccaacgtccg cccagcgaac tttttaccca
    23341 cgcgtagaca ccggcttctg agtttttatt taacgaaaca aattctgcga tgatcagaca
    23401 gaagggaata aaataaaaaa tcgtcgcgag gaaaaacatc ggtgccgagg ccagtccaag
    23461 ctcaatatta ttattgataa cgttgttaaa gctgtaaacg gcggcaaaag tcagcgagag
    23521 caagccgaat ttgccgattg tattacgttt ggtatcagac ataacacatc tccgaaaatg
    23581 tgtagggtag cggtggcggc aaactccttt tgccgccgta gttgttattt gttatggaaa
    23641 taaccatctt cgatggtgac tttgagaacc acttttttga ccgcgttatt gcagataaac
    23701 cgatacgctt catggatatc gcaaataacg atttgccctt cgtggacctc tacggtttct
    23761 ccgcagcctt ttaaatattc acggtcagtt tcatcacgat aatattccac tacctgtaat
    23821 gtctctttcg gcgcatattc aattttttgc tgcccttgca ggtagtaatg cacttcaaaa
    23881 tagcgtcgat gcccggtaaa tagcgcatcg gtcgcggagt cattttccac acggtaggtc
    23941 aatgaatcac cgatggagtg ggcgacgcca ggctgaatgt tgtcgatatt ttcaatcgct
    24001 tcaacgcagc gttgccactt cttgccagag gcgtaaatct ggcggaactg ttctaagtta
    24061 tcgatgatcc tcattgctta ttctcgctgt gcaaattcgt ggaaaagaac cctgcgccga
    24121 actcatacga cgccaggctt tgcgcggtag cttctccgcc agaaaccggc agcaacgtaa
    24181 agccgtagct gaagtcacgg aaccagacgc gccaggagtc cagcacctcg ctgccccagg
    24241 agttggagcc gaggccaagt agctggtggt cgaggttcag ggtgatgtca tcactgcgct
    24301 gcagctcgtt acagtgctgg gcagcgtgga tgttttcctg ggtatagtgc caggcgctga
    24361 agttaattgg gcgctgcgga accaccagca gaccgttgcc gtggcggtta gtcagtgccg
    24421 tccagcggac atgctgacgg ttgccgttgt tctgcgggaa gggatagttc tcgaacatgg
    24481 catcgacggt gctgcgccag atatcgatga tgttagcctg ctggctgtcg gcgtagtttt
    24541 cgcccggtcc acgaccgtaa tacgccacct gatcgtattc gccgttaatc cccatggtga
    24601 aaccgatgca cggaatgatg tgcggatagt cgccgtaacg ctcgccggaa agcgccacgt
    24661 taacctggcc atcggcagcg atgcgccaga tgtaggtaca gcgcatcccg aagtcaaaca
    24721 ccggcggtgc aataactgtg cggctgatga tcaacacttc accatcgctc tgttctacgg
    24781 caaagtcgcg cagatgttcc tgcatgatct gcaaatgatt cggctgccac aacccttcgt
    24841 actcctgctt gtggttgtcg atcatcggct tgaagaagtt gatctttggc tcgcgggtca
    24901 gcagcgactc gccatttacc tgccaggatg tcggtttgcc actcattttt gagaaggtga
    24961 tcgcgaagtt gtagccgcga acggtgcagc tcaaacgatc gtcttccagc gtcagcggac
    25021 gcgcattatt tggtgcgaaa ggcactggct gcgcggtgtt ttccttcagc gggaactgat
    25081 aagtggcgat agaatgcccg gcttcgctgt agcgggtgcg ggaatctttg gtcaccgtaa
    25141 tgttgaggaa cgcttcgcgg gcgtccagct gcggcagcgt gatctgcaag ggggcttcgc
    25201 tgttcggcgc aacgtcgcgc agtttaatct gctgcgtcgc gagcgtttca ccttcggcgc
    25261 gcacctctgc gtgcagggtg tagtcatcaa gcgtggtaaa ccacagttta ttttcgactt
    25321 tcaactcgcc gcgagtcagc gcgtggattt ttaccggcgc gataacctgt ttgtactctt
    25381 tcaggcccga tcccggcgtc tgatcggaat agatcaaacc atcaagacag aagttatagt
    25441 tgttgggata gtcgccgtag tcgccgccga atttatacca gacattgccg tggtcgtcct
    25501 gtgcctggat cccgtggtcg caccactccc agacataatg accctgaatg caatcgtgct
    25561 tatagaagac gttctggtac tccgtcagcc cgcccggtcc gttccccatc gcatgagcat
    25621 attcacagat gatgcgcggc ttcggatgcg ggtattcacc aaactcattc atcagcggca
    25681 cgcgggtgta catggtggaa ataatatcga ccacttcagc atcgcgatct tcttcgtaat
    25741 gaaccagtcg cgtgtcatcc agcgctttcg ccgcatggta catcgcgcgg atgttacagc
    25801 catagccgga ttcattgccc agcgaccaga tgatgatcga cggatggttt ttctgtgcgt
    25861 ggatatggcg aacaatgcgc tcgacgtaga ctttttccca ctgcggatcg tcggtaatac
    25921 ggctgatatc gccgacatta gcaaagccgt gcgattcgac gtcggtttcc gccatcacaa
    25981 acaggccgta gatatcacac agttcgtaaa aacgcggatc gttcgggtag tgagcggtac
    26041 gcacggagtt gatattgtgc tgcttcatca actggagatc tttctcgacg cgatccattc
    26101 caacggcgcg gcctttgcga tgatcgttgt cgtgacggtt gacgccgtgc agcatcacat
    26161 aacggttatt gatccagaac agaccgtcgc gcactttgat atcacggaag ccaacgcgtt
    26221 gtggcaccac ttccagaacg ttgccgttgg cgtctttcag cgtcatgacc agatggtaaa
    26281 gataagggga ttctgctgac cattgctgcg gctgttcgac agtaaaagca aagctggcgc
    26341 tggtcagttt ttcaattgcc aaatgatcaa tggcgctgct gtgcaccacg cgttcgccat
    26401 caaacagggt atattccagc gtcgtgacga caggggaggc ggcgagattt tccagcacca
    26461 cttcgcagga aagcgtggca tcgcaatagg cttcgtcaaa gtcggtacgc acagtgaaat
    26521 cgttaatatg cgttaggtgt tttccgacca gataaacatc gcggaagatc cccgccgacc
    26581 accacatatc ctggtcttcc acgtaggtag agtccgccca ctgcatcacg cgcacacaca
    26641 acaggttgtc gccggtttta accatcgcgc tgatgtcaaa ctctgcggtc aggcgactgc
    26701 ccttgctgaa acccacatac tgaccgttaa cgtagacttc aaaataggtt tcgacgccgt
    26761 caaatttaat cagcgtctgt ttaccctgcc agccgtcgct gagggtgaaa atacgttgat
    26821 aggcaccggt tgggttatcg ctggggacaa acggcacatc gatggggaac ggaaaacctt
    26881 cgtcggtata ttgcagtttg ccgtgacctt ccatttgcca catggcgggg acggtaatat
    26941 gcccccagtc agccattaac tcagaggtga aggcttctgg tacttgcagc ggatggtcaa
    27001 aaaagtggaa attccactga ctgcttaagg gcagaaacag gctgctggtt tcgcgggcaa
    27061 aggtacgcgc ttgcgcaaca gaatcatatg aaaaaaagta cgcacgcggc gcaagtcggt
    27121 tttcgtgggt gagctgaatg ttttcccagc gattcatagg gcctcccggt agagagaaat
    27181 cggaaccatt catggtgatg gtagtttgtg taattgctgg gggtttagta aaatttttac
    27241 taaaaagcag caggtaaatg catttacttt aactggatca cgaaaataaa cggcagcact
    27301 gtccggcaaa aatgattgcc gaacagtgtg taaggggatt taacgggtcg tgccgcgcag
    27361 ttttaatttg ctgggaacga agactagcag cggcagcgcg cgaccatcgc gggctttttc
    27421 atacaccagg ttaacgccct gacttcccat catctcggaa tggatgcgca cggtggagag
    27481 cggcggaaag gtaaatcgcg cggtggggat atcgttaacg ctgataagcg aaatatcttg
    27541 tgggatgttc aggccacgtt catgaattgc ccgcagtacg ccgatagcaa tggaatcgga
    27601 agcaacaaac agtgccttcg gatagtcttc ccgcgccagc atttgttttg ccagttcata
    27661 acccgacgaa ctggaaaaac cgccgcgcca gatatcctct tcgcgtacca cttgtttcag
    27721 tcggccatat tccgcaaagg cgacctcacg aatatccgcc ttgccaggct catcttcacc
    27781 gccaataaaa ccaatacgat taacgccctg gttgatatag aagtcgatga tttctttact
    27841 gatgcgtgcc agatcgatat ccaccgcatc gtaaccgctg ccgggttcgt gaaagtcgat
    27901 aaaacagata ttgtcggtca acgcgctggc agcggcgcgc agggcgggcg tgggtttgcc
    27961 gacaattaaa ataccggtga cgtttttaat gtctggtaat ccgttgtgtt cataacagtt
    28021 ggtcagttcg atcgccagct tttcgcactg agtttcaatg ccgtggcgga tcgccagata
    28081 gtaaggatcg ttgatctcca gctcctgctg gtagctgtag atagccagaa tatggtgttg
    28141 gttgactgcg cctgtctgta gtttacgggc gctactggtc ttgtactcca gcttttcggc
    28201 gatctcgaga atgcgatgtt tcgtctcttc tttcacattc aatgtcggat cgtcatttaa
    28261 gaccctggat actgtcgcca gggatacgcc agcttcgatt gcgatgtctt ttaatgttgc
    28321 catgtttacc ttttttactt tccctgatgt gcgcccccat tgtagaggaa gtgcttatcg
    28381 ggtcacctgt tttcagtaaa taaagcgcgt ttctttgacg ctgtaacggt aacacagcgg
    28441 cttaggaatg tgatcacgcc tgcattctcc atttttagcc tacaggattg agaaaaactt
    28501 ttctatagtt tagtaaaatt ttactcgaag ggaggaagca atggaaaccg tggcttacgc
    28561 tgattttgca cgtctggaaa tgcgcgtcgg aaagattgtg gaagtgaaac gccatgaaaa
    28621 cgccgacaag ctgtacatcg tacaggttga tgtggggaaa aaacactgca aaccgtgacc
    28681 agcctggtgc cgtactacag cgaagaggag ctgatgggga aaacggtggt ggtcttatgc
    28741 aatctgcaaa aagcgaaaat gcgtggtgaa acgtcggaat gcatgctgtt gtgtgcggaa
    28801 accgatgacg gcagtgaaag cgtgttgtta acgccggagc ggatgatgcc tgcgggcgta
    28861 cgcgtcgtat aaaaagatcg gatggcgacg tcgtatcgcc atccgatttg atattacgct
    28921 tcttcgacac ttactcgcat ggccgccagc gccttacgcg ccgctttgat caccagttca
    28981 cactgttcaa tggtcagtgt cagtggcggt tcaatgcgga tcgttttggc gttattgagc
    29041 gttccggcca ccagtacgcg ctggcggaac atctcgctgg caaagttata gccgatttcg
    29101 ttatcaacaa actcaatcgc catcaacatc cctttaccac gcgcttcctg taccagatcg
    29161 ggatattccc gcgccagttg acggaaaccg tccagcaaca tatcgccttt ttgctcagcc
    29221 tgagccggta agttctgctc cagcaacaca ttgatggtcg ccagcgccgc cgcacaggcc
    29281 agcgggttgc cgccaaaggt ggtggtatgc aggaatgggt tgtcgaacag aactgaaaac
    29341 acctcttcag tggcgatggt cgcgccaatc ggcatcacgc cgccgccgag ggcttttgcc
    29401 agacacagaa tgtccggctg aacgttctca tgttcgcagg caaacatctt gcccgtgcgc
    29461 cccatgcccg tttgtacttc atcgaggatc atcagtgcgc cgaactcatc gcacagctta
    29521 cgcacggcgg tgagatagcc cggcggcggc agaattacgc caccttcgcc ctgaatcggt
    29581 tcgaggatca ccgcagccac atcatcaccg gtttttttgc actcattaag agccgtgcgc
    29641 atggcttcga tattgccaaa cggcacatga cggaaccctg gcagcagtgg cataaacggt
    29701 ttgcggaagg tcgatttcgc cgtggccgac agcgcgccaa gtgatttacc gtggaacacg
    29761 ccgctggtgg caataaaagt aaatttgccg cgcggcgact gataagcctt cgccagcttc
    29821 agcgccgctt caacggactc ggtgccgcta ttacagaaga agctgtattt cagtttaccg
    29881 ggcgttagcg cagcaagggt tttcgccagc atagcccgta acggatcgag tagctcctgg
    29941 ctgtgcagtg gttgtttcgc aagttgattc tgtacggcgg aaaccacaac tggattacgg
    30001 tgccccacgt tgaaaattcc aaaacctccc aggcagtcga taaactcctg tccctgggtg
    30061 tcgacaagcg tatttaaacc tcccgcttgc cactctacgg ctccgtaatc cccgccggcg
    30121 gtaacagatt tgcgatactc taaaaacccc ggattgacat gctctttgaa gtattcaatc
    30181 acctctcggt taagtgcttt catctcctca tgatccagcg ttcgcttctc aatgagattc
    30241 agggcgtggg cgctgcacgc taaagccgat gcgctcgaag gtaacctgtt caaaatatgc
    30301 tccggaggct cgcgtatcac atgataccga tttaagtatt gcagggattg cgccactccg
    30361 gctgcgatcg cgaaaatcgg gataaacaca aatgcggtta tcatttgtgc aatatttaat
    30421 gctatccgtt aacatttgtt gttattttgc acggtttttg tcgggtatgg atttgatgat
    30481 tgctgcgcaa atgcacgttt tcagggcaat aattgcctga tttttgggca agataattaa
    30541 cgttttcgta tagtaacaga gtgaaagtat tttaaatcaa agaattagat ttgtgcttta
    30601 atcgcaaatt gcgatctaaa tcaaattaat cggttaaaga taaccgcagc ggggccgaca
    30661 taaactctga caagaagtta acaaccatat aacctgcaca ggacgcgaac atgtcttctc
    30721 atccgtatgt cacccagcaa aataccccgc tggcggacga taccactctg atgtccacta
    30781 ccgatctgca aagctatatc actcatgcta atgacacttt tgtgcaggtg agcggcttta
    30841 ccttgcaaga gttacaaggg cagccgcaca acatggtgcg tcacccggat atgccaaaag
    30901 cggcgtttgc ggatatgtgg ttcaccctga aaaaagggga gccctggagc ggcatcgtga
    30961 aaaatcgccg caaaaatggc gaccattatt gggtgcgggc caatgcggta ccgatggtgc
    31021 gcgagggaaa aatcagtggc tatatgtcga ttcgtacccg ggcgacggat gaagagattg
    31081 cggcggtgga gccgctgtac aaagcgttga actccggacg taccagtaag cgtattcata
    31141 aaggcctggt ggtgcgtaaa ggctggctgg gtaaactgcc ttcattaccg cttcgctggc
    31201 ggacgcgtgg agtgatgacc ctgatgttta tcttgctggc ggccatgctt tggtttgttg
    31261 ctgccccggt ggtgacgtat ttcctctgtg tgttagtggt attgttggca agcgcttgtt
    31321 ttgaatggca gattgtgcgc ccgatagaaa atgtcgcccg tcaggcactg aaggtggcga
    31381 ccggagagcg taatagtgtt gagcacctga atcgcagcga tgagctgggg ctgacattac
    31441 gcgcggtagg gtagcttggc ctgatgtgcc gttggctaat taacgatgtc tcaagccagg
    31501 tgtccagtgt cagaaatggc agtgagacgc tggcgaaagg caccgatgaa ctgaacgaac
    31561 atacccagca gacagttgat aacgttcagc aaacggtggc gaccatgaac caaatggcgg
    31621 cgtcggtgaa acagaactct gccacggcgt cggctgccga taaactttct atcaccgcca
    31681 gtaatgcggc agtgcagggt ggggaggcga tgaccacggt gatcaagaca atggacgata
    31741 tcgccgacag tacctagcgc attggcacca ttacttcgct gattaacgat attgcgtttc
    31801 agaccaatat tctggccctg aatgcggcgg tggaagcggc gcgtgccggc gaacagggca
    31861 aaggttttgc agtggtggca ggggaagtgc gtcatttagc cagccgcagc gctaatgctg
    31921 ccaacgatat tcgcaagctg attgatgcca gtgctgataa ggtgcaatcc ggttcgcagc
    31981 aggtacacgc cgccggacgg acgatggaag atattgtggc acaggtgaaa aacgtcaccc
    32041 agttgatcgc ccagattagc cattcaacgc tggaacaggc cgatgggctt tccagcctga
    32101 cccgtgcagt ggatgagctt aacctgatca cccagaaaaa tgccgagctg gtggaagaga
    32161 gtgcgcaggt gtcggcgatg gtgaaacacc gcgccagccg actggaagac gcggtgacgg
    32221 tgctgcatta atcggtatgc cggatccggc aattgcaccg cgccactggc ggatgcggcg
    32281 attaatccag ttgcgtaata tcaaaagcgg cgcggtcgat caccgcaatg atctttttta
    32341 tttgtgcatc actgatatcg ctctggttga cgcgtaaatc cagcaccgct ttaaaattat
    32401 ccagcgcccg cttcatttgc gggttctggc gcagcgccgc gccaacgcaa cgcgctttga
    32461 tgcgttcttc aatcatctcc acttgttcgc ggttttcttc cagccactgc gcgccttgtt
    32521 cggtcagcgc aatctgcttc ttaccacctt cctcttcgcg gatggtaatc agcgactgct
    32581 cctgcagaaa atccagcgtc gggtagatga cgcccgggct tggggtgtaa ttcccctggg
    32641 ttagattctc aatcgcttta atcaattcgt aaccgtggct gtcatcgcgc gagagaatat
    32701 ccagaatcac cagacgcaat tcaccgtgac caaagaaacg ttgccgacga ccgccgccgc
    32761 gaccgtggcg accaccgcaa cattgaccgt gttcatgctg gtgaccgtgt ccgcagtgct
    32821 cgtgttctga cttctcacct ttgcagcagc cctcatggcg tggctggcct tcatgtttac
    32881 aacacccttc gtgatgatgg ctcatatcag cctccttttg cttgattaga tatatctaat
    32941 ttatatctga tgtgagcatt actgcaagta caaaaatgat ttttaaacgt ggaagcacct
    33001 cacatctagg ccggataaga cgttcacgcc gcatccggca gcattgctat ggcactgaat
    33061 gccttcacat acttcagaca tatcagttct acaaatcgct tgcatttatc atgattaaca
    33121 atcattatca tttgcgaatt ttatttagat atatctgatt accatcacga aggcgataac
    33181 aatgaataac accccccgct acccgcagcg cgttcgcaat gatctgcgct tccgtgaact
    33241 gactgtgcta cgcgttgagc gcatcagcgc cggttttcag cgcattgtcc ttggcggcga
    33301 ggcgttggac ggttttacat cgcgcggctt tgacgatcac agcaaactct tctttcctca
    33361 acctgacgct cactttgtgc cgccaacggt aacggaagag agcatcgtct ggccggaagg
    33421 accacgccca ccgtcgcgtg actatacgcc actgtatgac gaactacgcc atgaactggc
    33481 gatcgatttc tttattcacg acggtggggt cgccagcggc tgggcgataa acttacggtg
    33541 gcaggtccgc gcggttcgct ggtggtgccg gaagattacg cgtatcagct gtatgtctgc
    33601 gatgaatccg gaatgcccgc actgcgccgc cgcctggaaa cgttgagcaa acttgccgtt
    33661 aaaccgcaag ttagtgcgct ggttagcgtg cgggataacg cctgtcagga ttatctcgcg
    33721 caccttgatg gttttaatat cgaatggctg gcacatgatg agcaggcggt agatgcgcgt
    33781 ctggcgcaga tgcaaatccc tgccgatgat tacttcatct ggataaccgg cgaaggtaaa
    33841 gtcgttaaga atttaagccg ccgctttgaa gcggaacagt atgacccaca gcgggtacgt
    33901 gcagcggctt actggcacgc aaagtaactg atatataaaa agaaaaaggc tgacgatttc
    33961 tcgtcagcct ttgctatatc tggtggcccc tgctggactt gaaccagcga ccaagcgatt
    34021 atgagtcgcc tgctctaacc actgagctaa ggggccatgg tagcggatta taaagtaact
    34081 ccgtgccgca atccagccat taccgcgcgc ctgctgtttt tataaacaat gtattttcaa
    34141 tccgttatac ttttcctggt gatgttagaa aggagtaaat atggttgagg atattttggc
    34201 tccagggtta cgggtcgtgt tttgcggtat caaccctggg ctttcatccg ccgggactgg
    34261 ttttcccttt gctcatccgg caaatcgctt ctggaaggtg atatatcagg ccgggtttac
    34321 cgaccgtcag ttgaagccgc aggaggcaca gcatctgctg gattatcgtt gtggcatcac
    34381 caaactggta gaccgtccaa cggtgcaagc caatgaagtt tcaaagcagg agctgcacgc
    34441 aggtgggcgt aagctgattg aaaaaattga agattatcag ccgcaggcgt tggctattct
    34501 gggcaaacaa gcatatgaac agggattcag ctagcgcggt gcacagtggg ggaaacaaac
    34561 gctcaccatt ggttcgacgc agatttgggt gctgccaaat cccagcggtt taagtcgcgt
    34621 ttcactggag aaactggttg aagcgtatcg cgagctggac caggcgctgg tagtgcgtgg
    34681 gcgataa
//
LOCUS       DAETQL010000040        34652 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.040, whole genome shotgun sequence.
ACCESSION   DAETQL010000040 DAETQL010000000
VERSION     DAETQL010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.040"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..332)
                     /locus_tag="K0W65_002074"
     CDS             complement(<1..332)
                     /locus_tag="K0W65_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095336.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
     gene            702..1652
                     /gene="corA"
                     /locus_tag="K0W65_002075"
     CDS             702..1652
                     /gene="corA"
                     /locus_tag="K0W65_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBI3095337.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1693..2073)
                     /locus_tag="K0W65_002076"
     CDS             complement(1693..2073)
                     /locus_tag="K0W65_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HBI3095338.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2087..2503)
                     /locus_tag="K0W65_002077"
     CDS             complement(2087..2503)
                     /locus_tag="K0W65_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095339.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="K0W65_002078"
     CDS             complement(2562..3452)
                     /gene="rarD"
                     /locus_tag="K0W65_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBI3095340.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3504..3971)
                     /locus_tag="K0W65_002079"
     CDS             complement(3504..3971)
                     /locus_tag="K0W65_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBI3095341.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4136..5005
                     /gene="pldA"
                     /locus_tag="K0W65_002080"
     CDS             4136..5005
                     /gene="pldA"
                     /locus_tag="K0W65_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBI3095342.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5138..6967
                     /gene="recQ"
                     /locus_tag="K0W65_002081"
     CDS             5138..6967
                     /gene="recQ"
                     /locus_tag="K0W65_002081"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBI3095343.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7031..7651
                     /gene="rhtC"
                     /locus_tag="K0W65_002082"
     CDS             7031..7651
                     /gene="rhtC"
                     /locus_tag="K0W65_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HBI3095344.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="K0W65_002083"
     CDS             complement(7713..8333)
                     /gene="rhtB"
                     /locus_tag="K0W65_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBI3095345.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8444..9466
                     /gene="pldB"
                     /locus_tag="K0W65_002084"
     CDS             8444..9466
                     /gene="pldB"
                     /locus_tag="K0W65_002084"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBI3095346.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9474..10274
                     /gene="yigL"
                     /locus_tag="K0W65_002085"
     CDS             9474..10274
                     /gene="yigL"
                     /locus_tag="K0W65_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBI3095347.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10350..11249
                     /gene="bioP"
                     /locus_tag="K0W65_002086"
     CDS             10350..11249
                     /gene="bioP"
                     /locus_tag="K0W65_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBI3095348.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11137..12090)
                     /gene="metR"
                     /locus_tag="K0W65_002087"
     CDS             complement(11137..12090)
                     /gene="metR"
                     /locus_tag="K0W65_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBI3095349.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12326..14587
                     /gene="metE"
                     /locus_tag="K0W65_002088"
     CDS             12326..14587
                     /gene="metE"
                     /locus_tag="K0W65_002088"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBI3095350.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14626..15441)
                     /locus_tag="K0W65_002089"
     CDS             complement(14626..15441)
                     /locus_tag="K0W65_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBI3095351.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15703..16464
                     /gene="udp"
                     /locus_tag="K0W65_002090"
     CDS             15703..16464
                     /gene="udp"
                     /locus_tag="K0W65_002090"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBI3095352.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16605..18032
                     /gene="rmuC"
                     /locus_tag="K0W65_002091"
     CDS             16605..18032
                     /gene="rmuC"
                     /locus_tag="K0W65_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBI3095353.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18127..18882
                     /gene="ubiE"
                     /locus_tag="K0W65_002092"
     CDS             18127..18882
                     /gene="ubiE"
                     /locus_tag="K0W65_002092"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBI3095354.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18896..19501
                     /gene="ubiJ"
                     /locus_tag="K0W65_002093"
     CDS             18896..19501
                     /gene="ubiJ"
                     /locus_tag="K0W65_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBI3095355.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19498..21138
                     /gene="ubiB"
                     /locus_tag="K0W65_002094"
     CDS             19498..21138
                     /gene="ubiB"
                     /locus_tag="K0W65_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBI3095356.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21217..21486
                     /gene="tatA"
                     /locus_tag="K0W65_002095"
     CDS             21217..21486
                     /gene="tatA"
                     /locus_tag="K0W65_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBI3095357.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21490..22005
                     /gene="tatB"
                     /locus_tag="K0W65_002096"
     CDS             21490..22005
                     /gene="tatB"
                     /locus_tag="K0W65_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBI3095358.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22008..22784
                     /gene="tatC"
                     /locus_tag="K0W65_002097"
     CDS             22008..22784
                     /gene="tatC"
                     /locus_tag="K0W65_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBI3095359.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22826..23608
                     /gene="tatD"
                     /locus_tag="K0W65_002098"
     CDS             22826..23608
                     /gene="tatD"
                     /locus_tag="K0W65_002098"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBI3095360.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="K0W65_002099"
     CDS             complement(23605..24093)
                     /gene="rfaH"
                     /locus_tag="K0W65_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBI3095361.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24260..25753
                     /gene="ubiD"
                     /locus_tag="K0W65_002100"
     CDS             24260..25753
                     /gene="ubiD"
                     /locus_tag="K0W65_002100"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBI3095362.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25799..26500
                     /gene="fre"
                     /locus_tag="K0W65_002101"
     CDS             25799..26500
                     /gene="fre"
                     /locus_tag="K0W65_002101"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBI3095363.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="K0W65_002102"
     CDS             complement(26692..27855)
                     /gene="fadA"
                     /locus_tag="K0W65_002102"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBI3095364.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="K0W65_002103"
     CDS             complement(27865..30054)
                     /gene="fadB"
                     /locus_tag="K0W65_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBI3095365.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30244..31575
                     /gene="pepQ"
                     /locus_tag="K0W65_002104"
     CDS             30244..31575
                     /gene="pepQ"
                     /locus_tag="K0W65_002104"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBI3095366.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31575..32189
                     /locus_tag="K0W65_002105"
     CDS             31575..32189
                     /locus_tag="K0W65_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBI3095367.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32228..33679
                     /gene="trkH"
                     /locus_tag="K0W65_002106"
     CDS             32228..33679
                     /gene="trkH"
                     /locus_tag="K0W65_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBI3095368.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33691..34236
                     /gene="hemG"
                     /locus_tag="K0W65_002107"
     CDS             33691..34236
                     /gene="hemG"
                     /locus_tag="K0W65_002107"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBI3095369.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8177 a         8737 c         9347 g         8391 t
ORIGIN      
        1 ttctgctgac cgttttcgat gtacaaaccg agcggcgcat agctttcatc atagatgccg
       61 ccgttcatcg ccatctgcac ctgaccctga ctattaatat ccgccagcag agcatgtaac
      121 gttccccacg cttcgccatt ggctttttgc cagtacattt tcacccgctc tgtttgagga
      181 ttaacggtat acgcctgtac ggtcagcgtc ggatctgaga gtgcgcaatc atcagcggca
      241 acagcaaaca agggaagtaa ggtgagggtg agaaaaatcc gtttgagatt caaggtgatc
      301 attcctttac caatgagtag ccgatgcgcc attataggtc ctagatgtgg gattttttta
      361 tcctgttagc gaccttgacg agtaccaaaa agcgcgaagt tcaactattg ttctgtggtg
      421 ttctgttgcg tgttgacggc aaaattttgc tggcgtaaca tgcgcgcacg atcactctaa
      481 gaggacattc gccttggaca cacccagtag atactggctc actatcctgt catccaggat
      541 caactcctaa ggctatccct ttttgctgat agccttagcg gttgtcagcg acctcaattt
      601 ttcccgtcgc gctgagtcag gctgtttaat ggtctgaaac ccaatttgtt tctgtgtgcc
      661 caccgaactg tccgatattt taagcattgg gagtcccggt catgctgagc gcatttcaac
      721 tggaaaataa ccgactgacc cggctggaag tcgaagagtc acaacccctt gtaaatgcag
      781 tatggattga tcttgtcgaa ccggacgacg acgagcgact gcgcgtacaa tctgaacttg
      841 gccagagcct ggcaacccgc ccggaactgg aagacatcga agcatcggca cgtttctttg
      901 aagacgacga cggcctgcat attcactcct tcttcttctt tgaagatgcg gaagatcacg
      961 ccggtaactc cactgtggca tttaccatcc gtgatggtcg tctgtttact ctgcgtgagc
     1021 gtgaactgcc cgcttttcgt ctgtatcgta tgcgtgcccg tagccagtcg atggtagacg
     1081 gtaacgccta cgagttgctg ctggatctgt tcgaaaccaa aatcgaacag ttggcagatg
     1141 aaattgaaaa tatctatagc gacctggagc agttgagccg ggtgattatg gaagggcatc
     1201 agggcgatga gtacgacgag gcgctctcca cgctggcgga actggaagat atcggctgga
     1261 aagttcgcct gtgtctgatg gatacccagc gcgctctcaa cttcctggtg cgtaaagcgc
     1321 gtttaccggg tgggcaactg gagcaggcgc gtgaaatcct gcgcgatatc gaatccctgc
     1381 tgccgcataa cgaatccctg ttccagaagg tgaacttcct gatgcaggcg gcaatgggtt
     1441 ttatcaacat cgagcagaac cgcatcatca aaattttctc ggtggtatcc gtcgtgttcc
     1501 tgccgccaac gctggttgct tccagctacg ggatgaactt tgagtttatg ccagaactga
     1561 agtggagttt cggctaccct ggcgcgatta tctttatgat cctcgcgggc ctggcaccgt
     1621 atctgtactt taagcggaag aactggttgt aaaaagaagg cggtggctta gcctggctaa
     1681 gccacctgtt attcaaaggc tccaggtatt taaccctttt acctctttct cataaaacca
     1741 tttgttcgtg ttaacagcaa cataggctgc tacggcaatt cccagaatgt taacgccaat
     1801 taatgcacca acaaatagag atagaatgcc aagtaataga actataattg cttttttcca
     1861 taagcccaga acaaataaat agatccagga acagaagaaa gcaatgaaat tcatttgaat
     1921 agttaatcgt tgtcttactt ttaatgcttt aaatgctgct ttatattctg gtgttgctcc
     1981 ccagaatcca ggaaagccat gttgatcata aaaattaaat cggtatttcc atttttcact
     2041 taatgaatca tcgttcatat attccttact cataaatact ccataactat tgttttgatg
     2101 aatcagtagg tgcaagcatt agcatactga aagtggaaaa ataacaaatc aaaaaaatca
     2161 tcgaaccatt gcctgaacag gcaaaatctt cggctatcat tgtgatgata gagatgatat
     2221 atactgctaa tgtaccaaaa acataaattt ttatatatat gaaaccacta tcacggagtc
     2281 gttggcaatt catgttgatg acgagataat ggagtgcgat ggtagagact ataacaagaa
     2341 aacctgcttc cccatcgtta aaaaagataa taagaaaggc aaaaatgaaa tttattaaaa
     2401 taaataaaaa tatatatcgg tgtctggaaa tcttaccgtt agatgttggg ataaacatac
     2461 gtaacataaa ttttacatcc ttgtgtgagt ctcctgtcag catggcaata tgcccactct
     2521 catacaagag tcggcatatt ttttcagaat atatttattt tttatttgga cgttctacgc
     2581 tgcgtataaa tcgcgtccat cacaaaaatt gccagcgcca cccaaataaa ggtgaaagtc
     2641 accatcttat cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc
     2701 gtcgggccaa tgtactgaaa aaagcctaac gttgagagac gcaggcgcgt ggcagcggca
     2761 gtaaaacaca acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc
     2821 gacatcgggt tttgccccat atggctggtt gaactgtcgg caatagcaaa caggtaaatc
     2881 gccgccacgg gcagcagcca catggtttca attaacatgc cggtttgtgc ttctaccgca
     2941 atcttcttgc gcaccaggcc gtagaaggca aaactaaatg ccagccccag cgcgataata
     3001 ggtagcgaac caaaagtcca cagctggacc aacacgccac atatcgccag aatcaccgcc
     3061 agccattgca tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc
     3121 agcgggttaa taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag
     3181 ataaacagta gccagttgcc accaatcagt acggcagaga ctgccagcat aaaaattttc
     3241 tgtggcgtct gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc
     3301 accataaaga aaaacgacca gatcacgcga tgcgtcagga tctcatcggc gggcacgtag
     3361 taaatcagct tgaagtatgc tggcgcaata ccccaaataa aataagcggc aagagcgagt
     3421 aatacgccct gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca
     3481 gcagtttacc tgcttttatg tcttcaacct accatatagg tggcggtggc actggcaata
     3541 taaagctgtt cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc
     3601 aacaggctac tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga
     3661 agatcaatgg tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg
     3721 tggcgggtta aggtacttcc cacgcacacc agaccggcgg cgacatccag cgccgacgca
     3781 atgactccgc cgtgcaaaat gctttgcgcc cagttaccca ccatcatcgg ctgattttta
     3841 aaggccagct gtgcgaactc cttttcgtaa cgctccagtt ccatccccaa tgcgcggtta
     3901 aatggcatgt gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt
     3961 acggcagaca tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt
     4021 tgttgttggt ttctacttta ggaagggata actaacggca atggagttaa gtatgtaaaa
     4081 tagcccgcag aaaaacattc accttatcaa taattcgtta cggagaacac gaccgatgcg
     4141 gactctgcag ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga
     4201 ggcaacggtg aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat
     4261 gctgcaggag catgacaatc cgttcacgct ctatccttat gacaccaact acctcatcta
     4321 cacccaaacc agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc
     4381 gcgtaaggat gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt
     4441 aggcccaaac tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa
     4501 tagcgaagag tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt
     4561 tgccaccgat taccgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca
     4621 cgactctaac gggcgttcag acccgacctc ccgcagctgg aaccgccttt atactcgcct
     4681 gatggcagaa aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac
     4741 tgacgataac ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca
     4801 cctcggtgat gcggtgctca gtgcgaaagg acagtacaac tggaacaccg gctacggcgg
     4861 cgcggagtta ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta
     4921 cagcggctat ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg
     4981 ggttatgcta aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaatccag
     5041 catccaggat taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa
     5101 atagcgcctg tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa
     5161 tctggagtcc ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc
     5221 cggccaggaa gaaattatcg acactgtgct ttccggtcgc gattgcctgg ttgttatgcc
     5281 cactggtggc ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac
     5341 cgtggttgtt tcaccgctga tttcgttgat gaaagatcag gtggatcaac tacaggccaa
     5401 cggcgtggcg gcggcgtgcc ttaactcgac gcaaacccgc gaacagcaac ttgaagtgat
     5461 gacaggctgc cgcaccgggc aaattcgctt actgtatatc gccccggaac gcctgatgct
     5521 ggataacttt cttgagcatc tggcgcactg gaatccggtg ttattagccg tcgatgaagc
     5581 gcactgtatc tcccaatggg gtcacgattt ccgcccggaa tatgccgcgc tcggtcagtt
     5641 gcgccagcgg ttcccgacgc tgccgtttat ggcgctgacc gccacagccg acgacaccac
     5701 gcgccaggat atcgtgcgcc tgctggggct gaacgatccg ctgattcaaa tcagcagttt
     5761 tgaccgtccg aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat
     5821 gcgctacgtg caggaacagc gcggtaagtc tggcattatc tactgcaaca gccgggcgaa
     5881 agtagaagac accgctgcgc gcctgcaaag caggggtatt agcgcggcgg cctatcatgc
     5941 cgggctggaa aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgatctgca
     6001 aattgtggtg gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt
     6061 ggtccacttt gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg
     6121 gcgtgatggc ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct
     6181 gcgccgttgt ctggaagaga agccgcaggg gcagttgcag gatatcgagc gccacaaact
     6241 caatgcgatg ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta
     6301 ttttggcgaa gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa
     6361 acagtacgac ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca
     6421 gcggtttggg atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg
     6481 cgactatggt catgacaaac tgaaagtcta tggtatgggc cgtgataaaa gccatgaaca
     6541 ttgggtgagc gtgatccgcc agctgattca tctcggcctg gtgacgcaaa atattgccca
     6601 gcattctgcc ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt
     6661 gcaacttgcc gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg
     6721 cggcaactat gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga
     6781 tgaaagtaat gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga
     6841 acagatgccg atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct
     6901 ggaacgcttt ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga
     6961 agagtagtca gcagcataaa aaagtgccag tatgaagact ccgtaaacgt ttcccccgcg
     7021 agtcaaatgt atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat
     7081 gagccccggt cccgatttct tttttgtctc tcagaccgct gtcagtcgtt cccgtaaaga
     7141 agcgatgatg ggcgtgctgg gcattacctg cggcgtaatg gtttgggctg ggattgcgtt
     7201 gcttggcctg catttgatta tcgaaaaaat ggcctggctg catacgctga ttatggtagg
     7261 cggtggcctg tatctctgct ggatgggtta ccagatgcta cgcggtgcac tgaaaaaaga
     7321 ggctgtatct gcgcctgcac cacaggttga gctggcgaaa agcgggcgca gtttcctgaa
     7381 aggcttactg accaatctcg ccaatccgaa agcgattatc tattttggtt cggtattctc
     7441 attgtttgtc ggcgataacg ttggcactac cgagcgctgg ggcattttcg cgttgattat
     7501 tgtcgaaaca ctggcgtggt ttaccgtcgt tgccagtctg tttgccctgc cgcaaatgcg
     7561 ccgtggttat caacgtctgg cgaagtggat tgatggtttt gccggggcgt tatttgccgg
     7621 atttggcatt catttgatta tttcgcggtg atgccagacg cgtcttcaga gtaagtcgga
     7681 taaggcgttt acgccgcatc cgacattatt tttcacgcat gcctcgccga tgctaacagc
     7741 gctcccacca gcataaacaa cgagccgaaa atcttattca gcgccttcat ctgctttggt
     7801 cctttaatcc atagagcaat cctttgagca agggtggcgt aaccgatcat cacaataata
     7861 tcgaccacaa tagtggtgac gccgagcacg atatactgca tcagttgcgg ctgttgcggc
     7921 atgatgaatt gcggaaatag cgccgccaga aacacaatac ttttgggatt ggtgagattc
     7981 acaaaaactg cgcgctggaa caaatgtcga cgcgattgag tagaggccag cgatttaaga
     8041 tcaattgcgc cagcggcgcg ccactgctgg attcccagcc aaatcaagta agccgcgcct
     8101 gcccacttta acacttcaaa cgcaatcact gagcgggaaa atagcgtccc caaccccacg
     8161 ccaaccagca caatatgaat cgccagtccg gtctgaagcc cagcaataga cgccaccgcg
     8221 ccgcgataac cgtggttgag cgaggtggtc atagtgttga ttgcaccaga gcctggcgac
     8281 aggcttaaaa tgatcgatgt cagcaggtag gcaaaccacc attctaaggt catgatgaac
     8341 tcccggtgtg tctatttttg tgccacaata cgctactgtc gcagcgttgt gtcaggcacg
     8401 cttaaaaaaa cgattttacg tggtttaaga ggcagattac ccgatgtttc agcagcaaaa
     8461 agactgggaa acaagagaaa acgcgtttgc tgcttttacc atgggaccgc tgactgattt
     8521 ctggcgtcag cgtgatgaag cagagtttac tggtgtggat gacattccgg tgcgctttgt
     8581 ccgttttcgc gcacagcacc atgaccgggt ggtagtcatc tgcccggggc gtattgagag
     8641 ctacgtaaaa tatgcggaac tggcctacga cctgttccat ttggggtttg atgtcttaat
     8701 catcgaccat cgcgggcagg gacgttccgg tcgcctgtta gccgatccgc atctcgggca
     8761 tgttaatcgc tttaatgatt atgttgatga tctggcggca ttctggcagc aggaggttca
     8821 gcccggtccg tggcgtaaac gctatatact ggcacattcg atgggcggtg cgatctccac
     8881 attatttctg caacgccatc caggtgtatg tgacgccatt gcgctaactg cgccaatgtt
     8941 tgggatcgtg attcgtatgc cgtcatttat ggcacggcag atcctcaact gggccgaagc
     9001 gcatccacgt ttccgtgatg gctatgcaat aggcaccggg cgctggcgcg cgttgccgtt
     9061 tgctatcaac gtactgaccc acagcagaca gcgatatcga cgtaacttac gcttctatgc
     9121 tgatgaccca acgattcgcg tcggtgggcc gacctaccat tgggtacgcg aaagtattct
     9181 ggctggcgaa caggtgttag ccggtgcggg tgatgacgcc acgcaaacgc ttctcttaca
     9241 agctgaagag gaacgcgtgg tggataaccg catgcacgac cgtttttgtg aactccgcac
     9301 cgccgcgggc catcctgtcg aaggaggacg gccgttggta ataaaaggtg cttaccatga
     9361 gatccttttt gaaaaggacg caatgcgctc agtcgcgctc cacgccatcg ttgatttttt
     9421 caacaggcat aactcaccca gcggaaaccg ctctacagag gtttaaattt cttatgtacc
     9481 aggttgttgc gtctgattta gatggcacgt tactttctcc cgaccatacg ttatcccctt
     9541 acgccaaaga aactctgaag ctgctcaccg cgcgcggcat caactttgtg tttgcgaccg
     9601 gtcgtcacca cgttgatgtg gggcaaattc gcgataatct ggagattaag tcttacatga
     9661 ttacctccaa tggtgcgcgc gttcacgatc tggatggcaa tctgattttt gctcataacc
     9721 tggatcgcga cattgccagc gatctgtttg gcgtagtcaa cgacaatccg gacatcatta
     9781 ctaacgttta tcgcgacgac gaatggttta tgaatcgcca tcgcccggaa gagatgcgct
     9841 tttttaaaga agcggtgttc aaatatgcgc tgtatgagcc gggattactg gagccggaag
     9901 gcatcagcaa agtgtttttc acctgcgatt cccatgaaca actgctgccg ctggagcagg
     9961 cgattaacgc tcgctggggc gatcgcgtca acgtcagttt ctctacctta acctgtctgg
    10021 aagtgatggc gggcggcgtg tcgaaaggcc atgcgctgga agcagtggcg aagaaactgg
    10081 gctacagcct gaaggattgt attgcgtttg gtgacgggat gaacgacgcc gaaatgctgt
    10141 cgatggcggg gaaaggctgc attatgggca gtgcgcacca gcgtctgaaa gatctgcatc
    10201 cggagctgga agttattggt actaatgccg acgacgcggt gccgcattat ctgcgtaaac
    10261 tctatttatc gtaatcgttc tttatttggt cagttgtcaa cctgatactt cgctacaatg
    10321 gatactcgtt aatcaaagag ttttccattg tggcgttact tatcatcacc acgattctgt
    10381 gggccttctc ctttagcttt tatggcgagt accttgcggg gcacgtcgat agctattttg
    10441 cggtgctggt gcgcgttggc ttggcggcac tcgtttttct gccgtttctg cgtacccgta
    10501 gcaatagcct gaaaacggtc ggcctgtata tgctggtggg cgcgatgcag ctcggcgtga
    10561 tgtatatgct gagtttccgc gcttatctct acctgacggt ctccgaattg ttgctgttca
    10621 ccgtactgac gccgctctac atcacgctga tttatgacat catgagtaag cgccgtctgc
    10681 gctggggcta tgcttttagc gccttgctgg cggtgattgg tgccgggatt attcgctatg
    10741 atcaggtcac cgaccatttc tggactggct tgttgctggt gcaactctcc aatatcactt
    10801 ttgccatcgg catggtgggt tacaaacgcc tgatggaaac tcgcccgatg ccgcagtata
    10861 acgcctttgc gtggttctat cttggcgcgt tcctggtggc ggtaattgca tggttcttgc
    10921 tgggaaatgc gcagaaaatg ccgcaaacca cgttgcaatg gggcattctg gtgtttcttg
    10981 gcgtggtggc ttccgggatt ggctacttta tgtggaacta cggcgcgacg caggtggacg
    11041 ccggaacgct gggcattatg aataatatgc acgttccggc agggctgctg gtaaacctgg
    11101 ctatctggca ccaacagccg cactggccaa cgtttattac aggcgcgctg gtgatcctgg
    11161 cctcactgtg ggtgcatcgt aagtgggtcg ctccgcgctc ttcacaaacg gcagatgatc
    11221 gcaggcgtga ttgcgcgctg agcgaataaa cgcttccgta actggctgac gctgctcgcc
    11281 atcgcgcacg gcggcgtaca gtcggctcca caagccttcg cccagggttt tggtcaccac
    11341 cagaccctgg cgctcaaaac tctctactac ccaatgcggt agcgcggcaa tacccatccg
    11401 cgcggcaacc atttgaatca acaataaggt gttatcgacg cttttcagtg acgggctgac
    11461 gcctgccggc tgaagaaaat gccgccagac atccagtcga ctacgctgca ccggataaat
    11521 taatagcgtc tcgctggcga gatcttccgg tgtaatacgc gtttttgccg ccagtggatg
    11581 gtcaggtgct aacaccaggc gtacttcata atcgaacatc ggcgaataat gcaggccact
    11641 gcgcggcagg atatcggagg tcatcaccag atccagctcg ccctgttgca acgcgggctg
    11701 cgggtcaaac gtcacgcccg atttaaaatc catctctacc tgcggccagt tcttatggaa
    11761 atttcctaaa gcgggtgtca gccactgaat acagctatgg cactcaatgg caatgcgcag
    11821 acgcgtctgc tgcggttcat tgcaggcttg cagtgcctgg ctgatttgcg gcagcacctg
    11881 gtttgccagt tgcaacagga tttctccctg cggtgtaaag cgtagcggct ggctcttacg
    11941 cacaaatagc cggaagccaa ggcgttgttc cagatcgcta aactggtgag acagggcgga
    12001 ttgcgtctga tgcaacgttg ccgcagcggc tgcgagcgag ccgcagttcc gcaacgcttg
    12061 tagcgttttc aggtgtttta cttcgatcat gaaagtcctt cacttcggca tgaataattt
    12121 gcgcttgagg aatatacagt aaccgccaat tatggatgtg taaacatctg gacggctaaa
    12181 atcattcgtc ttttaaattt atggtgcgtt ggctgcgttt ctccaccccg gtcacttact
    12241 tcagtaagct cccggcgatg aataaacttg ccgccttccc taaattcaaa atccatagga
    12301 tttacatata attagaggaa gaaaaatgac aatattgaat cacaccctcg gtttccctcg
    12361 cgttggcctg cgtcgcgagc tgaaaaaagc gcaagaaagt tattgggcgg ggaactccac
    12421 gcgtgaagaa ctgctgacag tagggcgtga actgcgtgct cgtcactggg atcaacaaaa
    12481 gcaagcgggt atcgacctgc tgccggtggg cgattttgcc tggtacgatc atgtactgac
    12541 caccagtttg ctgctgggta atgttccgcc acgtcatcag aacaaagacg gttcggtaga
    12601 tatcgacacc ctgttccgta ttggtcgtgg acgtgcgccg actggcgaac ctgcggcggc
    12661 agcggaaatg accaaatggt ttaacaccaa ctatcactac atggtgccgg agttcgttaa
    12721 aggccaacag ttcaaactga cttggacgca gctgctggag gaagtggacg aggcgctggc
    12781 gctgagccac aacgttaagc ctgtgctgct ggggccggtt acttacctgt ggctggggaa
    12841 agtgaaaggt gaacagtttg accgccttag cctgctgaac gacattctgc cggtttatca
    12901 gcaagtgctg gcagaactgg cgaaacgcgg catcgagtgg gtacagattg atgaacccgc
    12961 gctggtactg gaactaccac aggcgtggct ggacgcatac aaacccgctt acgacgcgct
    13021 ccagggacag gtgaaactgc tgctgaccac ctattttgaa ggcgtaacgc caaatctcga
    13081 cacgattact gcgctgcctg ttcaggggct gcatgtggac cttgtacatg gtaaagataa
    13141 cgttgttgaa ctgcacaagc gcctgccttc tgactggctg ctgtctgcgg gtctgatcaa
    13201 tggtcgtaac gtctggcgcg ccgatcttac tgagaaatat gcgcaaatta aggacattgt
    13261 cggcaaacgt gatttgtggg tggcatcttc ctgctcgttg ctgcacagcc ccatcgacct
    13321 gagcgtggaa acgcgtcttg atgcagaagt gaaaagctgg tttgccttcg ccctacaaaa
    13381 atgccatgaa ctggcactgc tgcgcgatgc gctgaacagt ggtgacacgg cagctctggc
    13441 agagtggagc gccccaattc aggcgcgtcg tcactctact cgtgtacata atccggcggt
    13501 agaaaagcgt ctggcggcga tcaccgccca ggacagccag cgtgcgaatg tctatgaagt
    13561 gcgtgctgaa gcccagcgtg cgcgttttaa actgccagcg tggccgacca ccacgattgg
    13621 ttccttcccg caaaccacgg aaattcgtac cctgcgtctg gatttcaaaa agggcaatct
    13681 cgacgccaac aactaccgca cgggcattgc ggaacatatc aagcaggcca ttgttgagca
    13741 ggaacgtttg ggactggatg tgctggtaca tggcgaggcc gagcgtaatg acatggtgga
    13801 atactttggc gagcacctcg acggatttgt ctttacgcaa aacggttggg tacagagcta
    13861 cggttcccgc tgcgtgaagc caccgattgt cattggtgac attagccgcc cggcaccgat
    13921 taccgtggag tgggcgaagt atgcgcaatc gctgaccgac aaaccggtga aagggatgct
    13981 gacggggccg gtgaccatac tctgctggtc gttcccgcgt gaagatgtca gccgtgaaac
    14041 catcgccaaa cagattgcgc tggcgctgcg tgatgaagtg gccgatctgg aagccgctgg
    14101 aattggcatc atccagattg acgaaccggc gctgcgcgaa ggtttaccgc tgcgtcgtag
    14161 cgactgggat gcgtatctcc agtggggcgt agaggccttc cgtatcaacg ccgccgtggc
    14221 gaaagatgac acacaaatcc acactcacat gtgttattgc gagttcaacg acatcatgga
    14281 ttcgattgcg gcgctggacg cagacgtcat caccatcgaa acctcgcgtt ccgacatgga
    14341 gttgctggag tcgtttgaag agtttgatta tccaaatgaa atcggtcctg gcgtctatga
    14401 cattcactcg ccaaacgtac cgagcgtgga atggattgaa gccttgctga agaaagcggc
    14461 aaaacgcatt ccggcagagc gcctgtgggt caacccggac tgtggcctga aaacgcgcgg
    14521 ctggccagaa acccgcgcgg cactggcgaa catggtgcag gcggcgcaga atttgcgtcg
    14581 ggggtaaatc caaaccgggt ggtaatacca cccggtcttt tctcattaca gcgacttctt
    14641 cccaccatac tgcttaaacc attccagcat acgctgccag ccatctttcg cagattcggc
    14701 atgatagctc gggcgataat cagcgttgaa tgcatgcccg gcatccgggt acacgataat
    14761 ctctgctttc gcattagcag cccgcagcgc ctggcgcatc gtttcaacgc tctcctgcgg
    14821 aatgctgtta tcctgaccac catataagcc gagaaccggc gcgttaagat cggttgcgat
    14881 atcaacaggt tgtttcggtg aattcagcga cttgtcgccc gtcagtttgc cgtaccacgc
    14941 cactgcggct tttagctgtg gattatgcgc ggcatacagc caggtgatac gtccacccca
    15001 gcagaatccg gtgatcatta aacgatgaac atcgccgccg ttgcgggaag cccaactggc
    15061 gacatgatcg agatcggcca gcacctgcga gtcaggcact tttgctacca gaccgctaag
    15121 caacgtgggg atatcggcaa aatcattcgg atcgccttcg cggaagtaaa gttcaggtgc
    15181 gatagccaga tacccctcca gcgccagacg gcgacaaatg tcgcggatat gttcatgcac
    15241 gccaaaaatt tcctgcacta caatgaccac tggcagtggg ccatcgcttt gctttggtct
    15301 ggcatggtaa gcaggcatgt tatccccttg tgaagggata gaggtgaagc ccgccacaat
    15361 cgcgtcgtcc ggggtctgaa cgatggtcga agcgagaggc gatgcagcag gtgcaaatcc
    15421 agattgttgt gttgttgcca tggtattctc cgtaccctta taaaaatgtt gcgcaatgtt
    15481 aactatagtc agcatgcaac aaatcacatt gcctgaatcg gctcatcttt tatgcagtcc
    15541 tgcagaatga agagtgattt atgtgatatg aatcactttt ggtgggtaaa tttatgcaac
    15601 gcatttgcgt catggtgatg agtatcacga aaaaatgtta aacccttcgg taaagtgtct
    15661 ttttgcttct tctgactaaa ccgattcaca gaggagttgt atatgtccaa gtctgatgtt
    15721 tttcatctcg gcctcactaa aaacgattta caaggggcta cgcttgccat cgtccctggc
    15781 gacccggatc gtgtggaaaa gatcgccgcg ctgatggata agccggttaa gctggcatct
    15841 caccgcgaat tcactacctg gcgtgcagag ctggatggta aacctgttat cgtctgctct
    15901 accggtatcg gcggcccgtc tacctctatt gctgttgaag agctggcaca gctgggcatt
    15961 cgcaccttcc tgcgtatcgg tacaacgggc gctattcagc cgcatattaa tgtgggtgat
    16021 gtcctggtta ccacggcgtc tgtccgtctg gatggcgcga gcctgcactt cgcaccgctg
    16081 gaattcccgg ctgtcgctga tttcgaatgt acgactgcgc tggttgaagc tgcgaaatct
    16141 attggcgcga caactcacgt tggcgtgaca gtttcttctg ataccttcta cccaggtcag
    16201 gaacgttacg atacttactc tggtcgcgta gttcgtcact ttaaaggttc tatggaagag
    16261 tggcaggcga tgggcgtaat gaactatgaa atggaatctg caactctgct gaccatgtgt
    16321 gcaagtcagg gcctgcgtgc cggtatggta gcgggtgtta tcgttaaccg cacccagcaa
    16381 gagatcccga atgctgagac gatgaaacaa accgaaagcc atgcggtgaa aatcgtggtg
    16441 gaagcggcgc gtcgtctgct gtaattctct tctcctgtct gaaggccgac gcgttcggcc
    16501 ttttgtattt ttgcgtagcg cctcgcagga aatgcctttc caactggacg tttgtacagc
    16561 acaattctat tttgtgcggg taagttgttg cgtcaggagg cgttgtggat ttctcaatca
    16621 tggtttacgc agttattgcg ttggtgggtg tggcaattgg ctggctgttt gccagttatc
    16681 aacatgcgca gcaaaaagcc gagcaattag ctgaacgtga agagatggtc gcggagttaa
    16741 gcgcggcaaa acaacaaatt acccaaagcg agcactggcg tgcagagtgt gaattgctca
    16801 ataacgaagt gcgcagcctg caaagtatta acacctctct ggaggccgat ctgcgtgaag
    16861 taaccacgcg gatggaagcc gcacagcaac atgctgacga taaaattcgc cagatgatta
    16921 acagcgagca gcgcctcagt gagcagtttg aaaacctcgc caaccgtatt tttgagcaca
    16981 gcaatcgccg ggttgatgag caaaaccgtc agagtctgaa cagcctgttg tcgccgctac
    17041 gtgaacaact ggacggtttc cgccgtcagg ttcaggacag cttcggtaaa gaagcacaag
    17101 aacgccatac cctgacccac gaaattcgca atctccagca actcaacgcg caaatggccc
    17161 aggaagcgat caacctgacg cgcgcgctga aaggcgacaa taaaacccag ggcaactggg
    17221 gcgaggtagt attgacgcgg gtgctggagg cttccggtct gcgtgaaggg tatgaatatg
    17281 aaacccaggt cagcatcgaa aatgacgccc gctcgcggat gcagccggat gtcatcgtgc
    17341 ggctgccgca gggaaaagat gtggtgatcg acgccaaaat gacgctggtc gcctatgaac
    17401 gctattttaa cgccgaagac gactacaccc gcgaaagcgc gctacaggaa catatcgcgt
    17461 cggtgcgtaa ccatatccgt ttgctgggac gcaaagatta tcaacagctg ccggggctgc
    17521 gaactctgga ttacgtgctg atgtttattc ccgttgaacc cgctttttta ctggcgcttg
    17581 accgccagcc ggagctgatc accgaagcgt tgaaaaacaa tatcatgctg gttagcccga
    17641 ctacgctgct ggtggcgttg cgtactattg ccaacctgtg gcgttatgag catcaaagcc
    17701 gtaacgccca gcaaatcgcc gatcgtgcca gcaagctgta cgacaagatg cgtttgttca
    17761 tcgatgacat gtccgcgatt ggtcaaagtc tcgacaaagc gcaggataac taccgccagg
    17821 caatgaaaaa actctcttca ggtcgcggaa atgtgctggc gcaggcagaa gcgtttcgcg
    17881 gtttaggagt agaaattaaa cgcgagatta atccggattt ggctgaacag gcggtgagcc
    17941 aggatgaaga gtatcgactt cggtcggttc cggagcagcc gaatgatgaa gcttatcaac
    18001 gcgatgatga atataatcag cagtcgcgct agcccattag gggtagttaa gccgggtaga
    18061 aatctagggc atcgacgccc aatctgttac acttctggaa caattttttg atgagcaggc
    18121 attgagatgg tggataagtc acaagaaacg acgcactttg gttttcagac cgtcgcgaag
    18181 gaacaaaaag cggatatggt cgcccacgtt ttccattccg tggcatcaaa atacgatgtc
    18241 atgaatgatt tgatgtcatt tggtattcat cgtttgtgga agcgattcac gattgattgc
    18301 agcggcgtac gccgtgggca gaccgtgctg gatctggctg gtggcaccgg cgacctgaca
    18361 gcgaaattct cccgcctggt cggagaaact ggcaaagtgg tccttgctga tatcaatgaa
    18421 tccatgctca aaatgggacg cgagaagctg cgtaatatcg gtgtgattgg caacgttgag
    18481 tatgttcagg cgaacgctga ggcgctgccg ttcccggata acacctttga ttgcatcacc
    18541 atttcgtttg gtctgcgtaa cgtcaccgac aaagataaag cactgcgttc aatgtatcgc
    18601 gtgctgaaac ccggcggccg cctgctggtg cttgagttct cgaagccaat tatcgagccg
    18661 ctgagcaaag cctatgatgc atactccttc catgtgctgc cgcgtattgg ctcactggtc
    18721 gcgaacgacg ccgacagcta ccgttatctg gcagaatcca tccgtatgca tcccgatcag
    18781 gataccctga aagccatgat gcaggatgcc ggattcgaaa gtgtcgacta ctacaatctg
    18841 acggcagggg ttgtggcgct gcatcgtggt tataagttct gacaggagac cggaaatgcc
    18901 ttttaaacct ttagtgacgg caggaattga aagtctgctc aacaccttcc tgtatcgctc
    18961 acccgcgctg aaaacagccc gctcgcgtct gctgggtaaa gtattgcgcg tggaggtaaa
    19021 aggcttttcg acgtcattga ttctggtgtt cagcgaacgc caggttgatg tactgggcga
    19081 atgggcaggc gatgctgact gcaccgttat cgcctacgcc agtgtgttgc cgaaacttcg
    19141 cgatcgccag cagcttaccg cactgattcg cagtggtgag ctggaagtgc agggcgatat
    19201 tcaggtggtg caaaacttcg ttgcgctggc agatctggca gagttcgacc ctgcggaact
    19261 gctggcccct tataccggtg atatcgccgc tgaaggaatc agcaaagcca tgcgcggagg
    19321 cgcaaagttc ctgcatcacg gcattaagcg ccagcaacgt tatgtggcgg aagccattac
    19381 tgaagagtgg cgtatggcac ccggtccgct tgaagtggcc tggtttgcgg aagagacggc
    19441 tgccgtcgag cgtgctgttg atgccctgac caaacggctg gaaaaactgg aggctaaatg
    19501 acgccaggtg aagtacggcg cctatatttc atcattcgca cttttttaag ctacggactt
    19561 gatgaactga tccccaaaat gcgtatcacc ctgccgctac ggctatggcg atactcatta
    19621 ttctggatgc caaatcggca taaagacaaa cttttaggtg agcgactacg actggccctg
    19681 caagaactgg ggccggtttg gatcaagttc gggcaaatgt tatcaacccg ccgcgatctt
    19741 tttccaccgc atattgccga tcagctggcg ttattgcagg acaaagttgc tccgtttgat
    19801 ggcaagctgg cgaagcagca gattgaagct gcaatgggcg gcttgccggt agaagcgtgg
    19861 tttgacgatt ttgaaatcaa gccgctggct tctgcttcta tcgcccaggt tcataccgcg
    19921 cgattgaaat cgaatggtaa agaggtggtg attaaagtca tccgcccgga tattttgccg
    19981 gtcattaaag cggatctgaa acttatctac cgtctggctc gctgggtgcc gcgtttgctg
    20041 ccggatggtc gccgtctgcg cccaaccgaa gtggtgcgcg agtacgaaaa gaccttgatt
    20101 gatgaactga atttgctgcg ggaatctgcc aacgccattc agcttcggcg caattttgaa
    20161 gacagcccga tgctctacat cccggaagtt taccctgact attgtagtga agggatgatg
    20221 gtgatggagc gcatttacgg cattccggtg tctgatgttg cggcgctgga gaaaaacggc
    20281 actaacatga aattgctggc ggaacgcggc gtgcaggtgt tcttcactca ggtctttcgc
    20341 gacagctttt tccatgccga tatgcaccct ggcaacatct tcgtaagcta tgaacacccg
    20401 gaaaacccga aatatatcgg cattgattgc gggattgttg gctcgctaaa caaagaagat
    20461 aaacgctatc tggcagaaaa ctttatcgcc ttctttaatc gcgactatcg caaagtggca
    20521 gagctacacg tcgattctgg ctgggtgcca ccagatacca acgttgaaga gttcgaattt
    20581 gccattcgta cggtctgtga acctatcttt gagaaaccgc tggccgaaat ttcgtttgga
    20641 catgtactgt taaatctgtt taatacggcg cgtcgcttca atatggaagt gcagccgcaa
    20701 ctggtgttac tccagaaaac cctgctctat gtcgaagggg taggacgcca gctttatccg
    20761 cagctcgatt tatggaaaac agcgaagcct ttcctggagt cgtggattaa agatcaggtc
    20821 ggtattcctg ccctggtgag agcatttaaa gaaaaagcgc cgttctgggt cgaaaaaatg
    20881 ccagaactgc ctgaactggt ttacgacagt ttgcgccagg gcaagtattt acagcacagt
    20941 gttgataaga ttgcccgcga gcttcagtca aatcatgtac gtcagggaca atcgcgttat
    21001 tttctcggaa ttggcgctac gttagtatta agtggcacat tcttgttggt cagccgacct
    21061 gagtgggggc tgatgcccgg ctggttaatg gcaggtggtc tgatcgcctg gtttgtcggt
    21121 tggcgcaaaa cacgctgatt ttttcatcgc tcaaggcggg ccgtgtaacg tataatgcgg
    21181 ctttgtttaa tcatcatcta ccacagagga acatgtatgg gtggtatcag tatttggcag
    21241 ttattgatta ttgccgtcat cgttgtactg ctttttggca ccaaaaagct cggctccatc
    21301 ggttccgatc ttggtgcgtc gatcaaaggc tttaaaaaag caatgagcga tgatgaacca
    21361 aagcaggata aaaccagtca ggatgctgat tttactgcga aaactatcgc cgataagcag
    21421 gcggatacga atcaggaaca ggctaaaaca gaagacgcga agcgccacga taaagagcag
    21481 gtgtaatccg tgtttgatat cggttttagc gaactgctat tggtgttcat catcggcctc
    21541 gtcgttctgg ggccgcaacg actgcctgtg gcggtaaaaa cggtagcggg ctggattcgc
    21601 gcgttgcgtt cactggcgac aacggtgcag aacgaactga cccaggagtt aaaactccag
    21661 gagtttcagg acagtctgaa aaaggttgaa aaggcgagcc tcactaacct gacgcccgaa
    21721 ctgaaagcgt cgatggatga actacgccag gccgcggagt cgatgaagcg ttcctacgtt
    21781 gcaaacgatc ctgaaaaggc gagcgatgaa gcgcacacca tccataaccc ggtggtgaaa
    21841 gataatgaag ctgcgcatga gggcgtaacg cctgccgctg cacaaacgca ggccagttcg
    21901 ccggaacaga agccagaaac cacgccagag ccggtggtaa aacctgctgc ggacgctgaa
    21961 ccgaaaaccg ctgcaccttc cccttcgtcg agtgataaac cgtaaacatg tctgtagaag
    22021 atactcaacc gcttatcacg catctgattg agctgcgtaa gcgtctgctg aactgcatta
    22081 tcgcggtgat cgtgatattc ctgtgtctgg tctatttcgc caatgacatc tatcacttgg
    22141 tatccgcgcc attgatcaag cagttgccgc aaggttcaac gatgatcgcc accgacgtgg
    22201 cctcgccgtt ctttacgccg atcaagctga cctttatggt gtcgctgatt ctgtcagcgc
    22261 cggtgattct ctatcaggtg tgggcattta tcgccccagc gctgtataag catgaacgtc
    22321 gcctggtggt gccgctgctg gtttccagct ctctgctgtt ttatatcggc atggcattcg
    22381 cctactttgt ggtctttccg ctggcatttg gcttccttgc caataccgcg ccggaagggg
    22441 tgcaggtatc caccgacatc gccagctatt taagcttcgt tatggcgctg tttatggcgt
    22501 ttggtgtctc ctttgaagtg ccggtggcaa ttgtgctgct gtgctggatg gggattacct
    22561 cgtcagaaga cttacgcaaa aaacgcccgt atgtgctggt tggtgcattc gttgtcggga
    22621 tgttgctgac gccgccggat gtcttctcgc aaacgctatt ggcgatcccg atgtactgtc
    22681 tgtttgaaat cggtgtcttc ttctcacgct tttacgttgg taaagggcga aatcgggaag
    22741 aggaaaacga cgctgaagca gaaagcgaaa aaactgaaga ataaattcaa ccgcccgtca
    22801 gggcggttgt catatggagt acaggatgtt tgatatcggc gttaatttga ccagttcgca
    22861 atttgcgaaa gaccgtgatg atgttgtagc gcgcgctttt gacgcgggag ttaatgggct
    22921 actcatcacc ggcactaacc tgcgtgaaag ccagcaggcg caaaagctgg cgcgtcagta
    22981 ttcgtcctgt tggtcaacgg cgggcgtaca tcctcacgac agcagccagt ggcaagctgc
    23041 gactgaagaa gcgattattg agctggccgc gcagccagaa gtggtggcga ttggtgaatg
    23101 tggtctcgac tttaaccgta acttttcgac gccggaagag caggaacgcg cttttgttgc
    23161 tcagctacgc attgccgcag aattaaacat gccggtattt atgcactgtc gcgatgccca
    23221 cgagcggttt atgacattgc tggagccgtg gctggataaa ctgcctggtg cggttcttca
    23281 ttgctttacc ggcacacgcg aagagatgca ggcgtgcgtg gcgcgtggaa tttatatcgg
    23341 cattaccggt tgggtttgcg atgaacgacg cgggctggag ctgcgggaat tgttgccgtt
    23401 gattccggcg gagaaattgc tgatcgaaac agatgcgccg tatctgctcc cgcgcgatct
    23461 cacgccaaag ccatcatccc ggcgcaacga gccagcccat ctgccccata ttttgcaacg
    23521 tattgcgcac tggcgtggag aagatgccgc atggctggct gccaccacgg atgccaatgt
    23581 caaaacactg tttgggattg cgttttagag tttgcggaac tcggtgttct tcacactgtg
    23641 cttaatctct ttattaataa gattaagcaa tagcatggag cgagcctcac catcgggttc
    23701 ggtgaaaatg gcctgaaagc cttcgaacgc gccttcggta ataatcacct tatcacccgg
    23761 ataagggggg gccggatcga caatgtcttt cggtttatat accgatagct gatgaattac
    23821 cgccgatggg actatcgctg gcgacgcgcc aaagcgcaca aagtggctga cgccacgcgt
    23881 agcgctgatg gtcgtggtat gaatcacttc tggatcaaat tccacaaaca ggtagttagg
    23941 gaataacggt tcactgactg cggtacgttt accccgtacg attttttcca gggtgatcat
    24001 cggagcgagg caattaaccg cctgtctctc aaggtgttcc tgggcacgtt gaagttgccc
    24061 gcgcttgcag tacagtaaat accaggattg cataatgact cttatccgtt taatcggggc
    24121 gcaaggatag caaaagcttt acgctaagtt aattatattc cccggtttgc gttataccgt
    24181 cagagttcac gctaatttaa caaatttaca gcattgcaaa gatgaacgcc gtataatggg
    24241 cgcagattaa gaggctacaa tggacgccat gaaatataac gatttacgcg acttcctgac
    24301 gctgcttgaa cagcagggtg agctaaaacg tatcacgctc ccggtggatc cgcatctgga
    24361 aatcactgaa attgctgacc gcactctgcg tgctggtggg cctgcgctgt tgttcgaaaa
    24421 ccctaaaggc tactcaatgc cggtgctgtg caacctgttc ggtacgccaa agcgcgtggc
    24481 gatgggcatg gggcaggaag atgtttcggc gctgcgtgaa gttggtaaat tattggcgtt
    24541 tctgaaagag ccggagccgc caaaaggttt ccgcgacctg tttgataaac tgccgcagtt
    24601 taagcaggtg ttaaacatgc cgacaaagcg actgcgtggt gcgccctgcc aacaaaaaat
    24661 cgtctctggc gatgacgtcg atctcaatcg cattcccatt atgacctgct ggccggaaga
    24721 tgccgcgccg ctgattacct gggggctgac cgtaacgcgc ggcccgcata aagagcggca
    24781 gaatctgggc atttatcgcc agcagctgat tggtaaaaac aaactgatta tgcgctggct
    24841 gtcgcatcgc ggcggcgcgc tggattatca ggagtggtgt gcggcgcatc cgggcgaacg
    24901 tttcccggtt tctgtggcgc tgggtgccga tcctgccacg attctcggtg cagtcacccc
    24961 cgttccggat acgctttcag agtatgcgtt tgccggattg ctacgcggca ccaaaaccga
    25021 agtagtaaag tgtatttcca atgacctcga agtgccagcc agtgccgaaa tcgtcctgga
    25081 agggtatatc gatcctggtg agatggcgcc ggaagggccg tatggtgacc acacaggtta
    25141 ctataatgaa gtcgataatt tcccggtgtt taccgtgacg catattaccc agcgtgaaga
    25201 tgcgatttac cattccacct ataccgggcg tccgccagat gagcccgcgg tactgggcgt
    25261 ggcgttgaac gaagtgtttg taccgattct gcaaaaacag ttcccggaaa ttgtcgattt
    25321 ttacctgccg ccggaaggct gttcttatcg tctggcggta gtgacgatca aaaaacagta
    25381 cgccggacac gcgaagcgcg tcatgatggg cgtctggtcg ttcttacgcc agtttatgta
    25441 cactaaattt gtgatcgttt gcgatgatga cgtcaacgca cgcgactgga acgatgtgat
    25501 ttgggcgatt accacccgta tggacccggc gcgggatact gttctggtag aaaatacgcc
    25561 tattgattat ctggattttg cctcgcctgt ctctgggctg ggttcaaaaa tggggctgga
    25621 tgccacgaat aaatggccgg gggaaaccca gcgtgaatgg ggacgtccca tcaaaaaaga
    25681 tccagatgtt gtcgcgcata ttgacgccat ctgggatgaa ctggctattt ttaacaacgg
    25741 taaaagcgcc tgatgcgcgt ttgttttgcc ctatttatcg atccgacaga gaaagcgcat
    25801 gacaacctta agctgtaaag tgacctcggt agcagctatc acggataccg tatatcgtgt
    25861 ccgcatcgtg ccagacgcgg ccttttcttt tcgtgctggt cagtatttga tggtagtgat
    25921 ggatgagcgc gacaaacgtc cgttctcaat ggcctcgacg ccggatgaaa aagggtttat
    25981 cgagctgcat attggcgctt ctgaaatcaa cctttacgcg aaagcagtca tggaccgcat
    26041 cctcaaagat catcaaatcg tggtcgacct tccccacgga gaagcgtggc tgcgcgatga
    26101 tgaagagcgt ccgatgattt tgattgcggg cggcaccggg ttctcttatg cccgctcgat
    26161 tttgctgaca gcgttggcgc gtaacccaaa ccgtgatatc accatttact ggggcgggcg
    26221 tgaagagcag catctgtatg atctctgcga gcttgaggcg ctttcgttaa agcaccttgg
    26281 tctgcaagtg gtgccggtgg ttgaacaacc ggaagcaggc tggcgtgggc gtactggtac
    26341 cgtgttaacg gcggtattgc aggatcatgg tacgctggca gagcatgata tctatattgc
    26401 cggacgtttt gagatggcga aaattgcccg tgacctgttt tgcagtgagc gtaatgcgcg
    26461 ggaagatcgc ctgtttggcg atgcgtttgc atttatctga gatataaaaa aacccgcccc
    26521 tgacaggcgg gaagaacggc aactaaaact ttattcagtg gcatttagat ctatgtcgca
    26581 tccggcaatt ggtgcacgat gcctgatgcg acgctggcgc gtcttatcag gcctacattg
    26641 gtgccggatc ggtagaccgg ataaggcgtt cacgccgcat ccggcaagtg gttaaacccg
    26701 ctcaaacacc gtcgcaatac cctgacccag accgatacac atcgtcgcca gaccaaactg
    26761 aacgtctttg cgttccatca gattcagcag cgtggtgctg atacgcgcac cggaacaacc
    26821 cagcggatga cccagcgcga tcgcaccacc attgaggttg atcttctcgt caatctgctc
    26881 cattagcccg agatctttaa tgcacggcag gatctgcgcg gcaaaggctt cgttcatctc
    26941 aaacacgccg atatcgctgg cagaaagccc cgcttttttc agcgccagct tcgaggccgg
    27001 aaccgggccg taacccataa tcgatgggtc acaaccaacg accgccatcg aacgcacacg
    27061 agcgcgcggc ttaagaccta attcatgggc gcggctttca ctcatcacca gcatggcagc
    27121 tgcgccatcg gaaagtgcag aagatgtgcc cgccgttacc gtaccgttta ctggatcaaa
    27181 cgccggacgc agcgtggcga gggcttccac ggtggtttcc gggcgaatca cttcgtcgta
    27241 attaaactgc ttcaggacgc cgtcggcatc gtgaccacca gttgggatga tttcattttt
    27301 aaatgcggcc gactgcgtgg cggcccaggc gcgggcgtgt gaccgcgcgg caaaggcatc
    27361 ctgcatttca cggctgatac cgtgcatacg cgccagcatt tctgccgtta agcccatcat
    27421 gcccgccgct ttggcgacat tgcggctcag gccggggtga aaatcgacgc cgtgactcat
    27481 cggcacatgg cctatatgct ccacgccgcc aaccagacat gcctgcgtat cgccagtcat
    27541 gatcattcgt gctgcgtcat gcagtgcctg catggatgaa ccacacaagc gattgacggt
    27601 aaccgccggg acagagtgcg gcacttctgc cagcagcgcc gcgttacggg cgatgttaaa
    27661 accctgctcc agcgtctgtt gcacacaacc ccagtaaata tcgtcgaggg ccgccgcttc
    27721 cagcgccggg ttacgcgcca gcaggctacg cattaaatga gcggagagat cttccgcacg
    27781 cacgttacga aaagcaccgc ccttcgaacg gcccatcggg gtgcgaattg catcgacaat
    27841 gacaacctgt tccattgtga ctccttaagc cgttttcagg tcgccaaccg gacgggctgg
    27901 ctcaaccgga ggatagtacg gttcgttatg acgcgcttta ttacgcagac cttccggcac
    27961 ttcatacagc gggccgaggt gctgatattg ctgtgccata tcgaggtatt ttgcgctacc
    28021 gagggtgtcc agccagcgga acgcgccgcc gtggaacgga gggaagccca ggccgtagac
    28081 cagcgccata tccgcttccg ccggagtggc gataatgccc tcttccagac agcgcaccac
    28141 ttcgttgacc atcgggatca tcatgcgggc gataatctct tcttcgctga aatcgcgctt
    28201 cggttggctc acttctgcca gcaggtcttc aacggcggcg tcttcttctt tcttcggctt
    28261 acctttgctg tcttctttat aacgccagaa accgaggccg ttcttctgac caaagcggtt
    28321 ggcatcaaac agcgcgtcga tggcatcgcg gtaatctttc tgcatccgct gcgggaagcc
    28381 tgctgccatg acagcctgag cgtgatgcgc ggtatcaatg cccacaacgt ccagcagata
    28441 tgccgggccc atcggccagc caaactgttt ttccatcact ttgtcgatct tgcggaaatc
    28501 cgcgccgtcg cgcagcagct ggctgaaacc ggcgaaatac gggaacagca cgcggttaac
    28561 aaagaagccg gggcagtcgt taaccacaat cggcgtcttg cccatcttgc tcgcccaggc
    28621 gacaactttc gcgatggttt cgtcggagct tttctcgccg cgaataattt ctaccaacgg
    28681 cattcggtgg accgggttaa agaagtgcat cccgcagaag ttttccgggc gttccagcgc
    28741 gttggccagt tcgctgatag gaatggttga agtgttagac gccagcacgg tatcctggcg
    28801 tactttttgt tcggtttctg ccagtacggc ttttttcact ttcgggtttt caacaaccgc
    28861 ttctaccaca atatccacgc ggtcaaatcc ggcgtagtcg agcgttgggt ggatcgtgga
    28921 gatcacgcca gccagtttca gaccatcgat tttgccgcgc tcaagctgct tgttcagcag
    28981 tttcgcggct tcggtcatgc cgagggttaa cgacttgtcg ttgatatctt tcatgacaac
    29041 cggcacgcct ttccacgcgg actggtaagc gataccgcca cccatgatgc ctgcgcccag
    29101 cacagcagcc tgtttcgggg tttcaacgtc tttggtgagt ttcttcgctt tgccttttac
    29161 atattgatcg ttaaggaaaa tgccgaccag tgcgcgagct tcgttggtat gcgccagcgg
    29221 gacaaaactt ttgttttcca gatttaaggc ttcttcacga ccaaaacggg ccgcagcttc
    29281 aatggttttt actgcggtga tgggggccgg ataatgtttc cccgccgttt gcgcgaccat
    29341 tcctttagcg atggtgaagc tcatggtggc ttcaatcttg ctcagtttta gcggttcctg
    29401 cttcggctga cgtttcgctt tccagtcgag gtcgccgtta atggcctggc gtaaaatcgc
    29461 catcgcccct tcaatcagtt tttctgcttt gactacgcca tccaccagac cgattttcag
    29521 cgcctgatcc gcgccgacat ctttaccggc ggcaatgatt tccagtgcac tgtcagcgcc
    29581 cagcatacgt ggcatacgta cagaaccgcc aaagcccggc atgatgccca gtttggtttc
    29641 cggcagaccg atgcgcagat ccggcgtcgc cagacgataa tcggtcgcca gcacgcattc
    29701 gcagccacca cccagcgcat agccgttgac ggcagcaatg gtcggcaccg gcagatcttc
    29761 caggcgatta aacacgctat tggcaaagtg cagccactga cttaactgtt cttcaggaac
    29821 gaggaacagg gacaaaaatt cggtgatatc agcaccgacg ataaaggctg ctttgttcga
    29881 acgcagcagc agccctttta gatctgattg ctgttccagc acgccgatgg cctcgccgag
    29941 gctggcgacg gtcgcagtgt cgagtttatt aactgaacct ggggcatcaa ataccagttc
    30001 ggcaatgcca tcttccagcc agtcaaggta cagggtgtcg cctttgtaaa gcatgtcagt
    30061 ctcctgaatc cgcaaggtaa tctggtcgta ccagatgcat cggagtgtgt gttttgtgtt
    30121 aaaaatatgc aaataaaaga ttaaagaaat gccgatctga tcacgctcgg cagaaatcac
    30181 gctctggatg aacgatgtgc taagatgcgg agacttaagg tcaaaaaaac agaagggtaa
    30241 aaaatggaat cactggcctc gctctataaa aatcatatag ctaccttaca agaacggact
    30301 cgcgatgcgc tggcgcgctt caagctggat gcattactta ttcactccgg cgagctgttc
    30361 aacgtttttc tcgacgatca tccctatccg tttaaagtga acccgcaatt caaagcgtgg
    30421 gtgccggtaa ctcaggtgcc aaactgctgg ttgctggtgg atggcgtgaa caagccgaaa
    30481 ctgtggttct atctgccggt tgattactgg cacaacgtcg aaccgctgcc gacctccttc
    30541 tggactgaag atgtggaagt gatcgcgctg ccgaaagccg atggcattgg tagcctgctg
    30601 cctgctgcgc gcggcaatat cggttatatc ggtccggtgc cggaacgtgc gctgcaactg
    30661 ggtattgagg ccagcaacat caacccgaaa ggggtgatcg actacctgca ttattaccgc
    30721 tccttcaaaa ccgagtacga actggcctgt atgcgtgaag cgcagaaaat ggcggtcaat
    30781 ggtcatcgtg cggcagaaga agcgttccgt tctggcatga gcgagttcga tatcaatatt
    30841 gcctatctga ccgcgaccgg tcatcgtgat accgacgtac cttacagcaa cattgtggcg
    30901 cttaacgaac acgctgcggt gttgcattac accaaactgg accaccaggc accggaagag
    30961 atgcgcagct tcctgctgga tgccggggcc gaatataacg gctatgcggc tgacctgacc
    31021 cgtacctggt cggcaaaaag cgacaacgac tacgcacagc tggtgaaaga cgtaaatgat
    31081 gaacaactgg cgctgatcgc caccatgaaa gcaggcgtca gctatgtgga ttaccacatc
    31141 cagttccatc agcgcatcgc caaactgctg cgtaaacatc aaatcatcac cgatatgagt
    31201 gaagaagcga tggtcgaaaa cgatctcacc ggaccgttta tgccgcacgg tattggccat
    31261 ccgctgggcc tgcaggtgca tgacgtcgcc ggttttatgc aggatgatag cggtacacac
    31321 ctcgcggcac cggcaaaata tccgtacctg cgctacaccc gtattctcca gccgggcatg
    31381 gtgttaacca tcgaaccggg tatctacttc attgaatcgc ttctggcacc gtggcgtgaa
    31441 gggcagttca gcaagcactt caactggcag aaaattgaag cactgaaacc gttcggcggc
    31501 attcgtatcg aagacaacgt ggtgatccac gaaaacaacg tggaaaacat gacccgggat
    31561 ctgaaactgg cgtgatggaa agctggttaa ttcctgcggc accggtcacg gtcgttgaag
    31621 agatcaaaaa gagccgcttc ataacgatgt tggcgcatac cgatggcgtt gaggcggcga
    31681 aagcgtttgt tgaatcggtg cgggcagaac accccgatgc ccgccaccat tgcgtggcgt
    31741 gggtggcggg tgcgccggat gattctcaac agctgggttt ctctgacgac ggggagccgg
    31801 cgggaacggc aggtaaaccg atgctcgccc agctaatggg cagcggcgtc ggggaaatta
    31861 ccgctgtggt agtgcgctac tacggcggca tattgcttgg caccggtggg ttagtgaaag
    31921 cgtatggcgg cggcgtgaat caggcgctgc gccagctaac gacccaacgc aagacgccat
    31981 taaccgaata tactttgcaa tgtgaatata gtcagttaac cggcattgaa gcgttgctgg
    32041 ggcagtgtga cggcaaaatt atcaacagtg attatcaggc attcgttctg ctgcgggtgg
    32101 cgcttccggc ggcgaaagtg gctgaatttt ccgcaaagct ggcggatttt agccgtggtt
    32161 cattgcaatt gttagcgatt gaagaataat ccccacttcg ttttgcagaa ctaaggaagc
    32221 ggcagagatg cattttcgcg ccattacccg aatcgttgga ctactggtca tcttattttc
    32281 agggaccatg attatccctg ggctggtagc actcatctac cgggatggag cgggccgcgc
    32341 ttttacccag accttttttg tcgccctcgc cattggctct atgctgtggt ggccgaaccg
    32401 caaagagaag ggcgaactga aatcccgtga ggggtttctg atagtggtgc tgttttggac
    32461 cgtgctgggt agtgtcggtg cgctcccttt tatcttctcg gaaagcccga acctcacgat
    32521 taccgatgcg ttttttgaat ctttctctgg cctgaccacc accggggcca ctacgctggt
    32581 ggggctggat tcgctccctc atgccatcct cttttatcgc cagatgctgc aatggtttgg
    32641 cgggatgggg atcatcgtgt tagcggttgc gatactgcct atcctcggcg tgggtgggat
    32701 gcagctctat cgcgcagaaa tgcccggccc gctgaaagat aacaaaatgc gcccgcgaat
    32761 tgcggaaacg gcgaaaactc tgtggttgat ttatgtcttg ctgaccgtcg cctgtgcgct
    32821 ggcgttgtgg tttgccggaa tggatgcctt tgatgccatc ggccatagct ttgcgactat
    32881 cgctattggc ggcttctcga cacatgatgc cagtatcggt tatttcgaca gtccgactat
    32941 taacactatc attgctatct tcctgctgat ctccggctgt aactacggtc tgcacttttc
    33001 actgttaagt gggcgtagtc tgaaggttta ttggcgcgat ccggaatttc gcatgtttat
    33061 cggcgtacag tttacgctgg tggttatttg tacactcgta ctgtggtttc ataatgtcta
    33121 cagttcggcg ctgatgacaa ttaaccaggc gtttttccag gtggtatcga tggcgacaac
    33181 cgccgggttt acgactgaca gcattgcccg ctggccgctc tttttgccgg tactgctttt
    33241 atgttcagct tttatcggcg gttgcgccgg gtcaacgggc ggtggcctga aagtgatccg
    33301 catcctgctg ctgtttaagc aggggaaccg tgagctgaaa cgactggtgc atccgaacgc
    33361 cgtctatagc attaagctgg ggaatcgcgc actgccggaa cgtatcctcg aagccgtttg
    33421 gggatttttc tccgcctatg cattggtgtt tattgtcagt atgctggcga ttatcgccac
    33481 gggcgtggat gatttttctg cctttgcctc agttgttgcg acattgaata acctggggcc
    33541 agggcttggc gtggttgctg ataactttac cagtatgaac ccggtggcta aatggatcct
    33601 gattgccaac atgctgtttg gtcgtctcga ggtctttaca ttactggtgc tctttacccc
    33661 gactttctgg cgtgaatgat ggagtaatac gtgaaaacat taattctttt ctcaacaagg
    33721 gacggacaaa cgcgcgagat tgcctcctac ctggcttcgg aactgaaaga actggggatc
    33781 cagacggatg tcgccaatgt gcaccgcatt gaagaaccac aatgggaaaa ctatgaccgt
    33841 gtggtcattg gtgcttctat tcgctatggt cactaccact ctgcgttcca ggaatttgtc
    33901 aaaaaacatg cgacgcgact gaattcgatg ccgagcgcct tttattccgt aaacctggtg
    33961 gcgcgcaaac cggagaagcg tactccacag accaacagct acgcgcggaa gtttctgatg
    34021 aactcgcaat ggcgtcccga tcgctgcgcg gtcattgccg gggcgctgcg ttacccacgt
    34081 tatcgctggt acgaccgttt tatgctcaag ctgattatga agatgtcagg cggtgaaacg
    34141 gatacgcgca aagaagttgt ctataccgat tgggagcagg tggcgaattt cgcccgagaa
    34201 atcgcccatt taaccgacaa accgacgctg aaataagcat aaagaataaa aaatgcgcgg
    34261 tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat aatgcgccac
    34321 cactgacacg gaacaacggc aaacacgcag ccgggtcagc ggggttctcc tgagaattcc
    34381 gacagagaaa gcaaaaataa atgcttgact ttgtagcggg aaagcgtatt atgaataccc
    34441 cgcgccgctg agaaaaggcg aagcggcact gctctttaac aatttatcag acaatctgtg
    34501 tgggcactcg aagatacgga ttcttaacgt cgcaagacga aaaatgaata ccaagtctca
    34561 agagtgaaca cgtaattcat tacgaagttt aattctttga gcatcaaact tttaaattga
    34621 agagtttgat catggctcag attgaacgct gg
//
LOCUS       DAETQL010000041        34141 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.041, whole genome shotgun sequence.
ACCESSION   DAETQL010000041 DAETQL010000000
VERSION     DAETQL010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.041"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            679..816
                     /locus_tag="K0W65_002108"
     CDS             679..816
                     /locus_tag="K0W65_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095370.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1416..2195
                     /gene="ipaJ"
                     /locus_tag="K0W65_002109"
     CDS             1416..2195
                     /gene="ipaJ"
                     /locus_tag="K0W65_002109"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HBI3095371.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3138..4067)
                     /gene="virB"
                     /locus_tag="K0W65_002110"
     CDS             complement(3138..4067)
                     /gene="virB"
                     /locus_tag="K0W65_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HBI3095372.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4284..4520)
                     /locus_tag="K0W65_002111"
     CDS             complement(4284..4520)
                     /locus_tag="K0W65_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBI3095373.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(4524..6425)
                     /gene="ipaA"
                     /locus_tag="K0W65_002112"
     CDS             complement(4524..6425)
                     /gene="ipaA"
                     /locus_tag="K0W65_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HBI3095374.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6434..7432)
                     /gene="ipaD"
                     /locus_tag="K0W65_002113"
     CDS             complement(6434..7432)
                     /gene="ipaD"
                     /locus_tag="K0W65_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HBI3095375.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7483..8574)
                     /gene="ipaC"
                     /locus_tag="K0W65_002114"
     CDS             complement(7483..8574)
                     /gene="ipaC"
                     /locus_tag="K0W65_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HBI3095376.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(8594..10336)
                     /gene="ipaB"
                     /locus_tag="K0W65_002115"
     CDS             complement(8594..10336)
                     /gene="ipaB"
                     /locus_tag="K0W65_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HBI3095377.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10342..10809)
                     /gene="ipgC"
                     /locus_tag="K0W65_002116"
     CDS             complement(10342..10809)
                     /gene="ipgC"
                     /locus_tag="K0W65_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HBI3095378.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10866..11492)
                     /gene="ipgB1"
                     /locus_tag="K0W65_002117"
     CDS             complement(10866..11492)
                     /gene="ipgB1"
                     /locus_tag="K0W65_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HBI3095379.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11508..11897)
                     /gene="ipgA"
                     /locus_tag="K0W65_002118"
     CDS             complement(11508..11897)
                     /gene="ipgA"
                     /locus_tag="K0W65_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HBI3095380.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11910..13394)
                     /gene="icsB"
                     /locus_tag="K0W65_002119"
     CDS             complement(11910..13394)
                     /gene="icsB"
                     /locus_tag="K0W65_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HBI3095381.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13708..15324
                     /locus_tag="K0W65_002120"
     CDS             13708..15324
                     /locus_tag="K0W65_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HBI3095382.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAGVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15334..15696
                     /gene="ipgE"
                     /locus_tag="K0W65_002121"
     CDS             15334..15696
                     /gene="ipgE"
                     /locus_tag="K0W65_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HBI3095383.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15696..16154
                     /gene="ipgF"
                     /locus_tag="K0W65_002122"
     CDS             15696..16154
                     /gene="ipgF"
                     /locus_tag="K0W65_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HBI3095384.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16163..17278
                     /gene="mxiG"
                     /locus_tag="K0W65_002123"
     CDS             16163..17278
                     /gene="mxiG"
                     /locus_tag="K0W65_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HBI3095385.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17286..17537
                     /gene="mxiH"
                     /locus_tag="K0W65_002124"
     CDS             17286..17537
                     /gene="mxiH"
                     /locus_tag="K0W65_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HBI3095386.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17550..17843
                     /gene="mxiI"
                     /locus_tag="K0W65_002125"
     CDS             17550..17843
                     /gene="mxiI"
                     /locus_tag="K0W65_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HBI3095387.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17849..18574
                     /gene="mxiJ"
                     /locus_tag="K0W65_002126"
     CDS             17849..18574
                     /gene="mxiJ"
                     /locus_tag="K0W65_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HBI3095388.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18571..19098
                     /locus_tag="K0W65_002127"
     CDS             18571..19098
                     /locus_tag="K0W65_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HBI3095389.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            19040..19735
                     /gene="mxiN"
                     /locus_tag="K0W65_002128"
     CDS             19040..19735
                     /gene="mxiN"
                     /locus_tag="K0W65_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HBI3095390.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19728..20135
                     /gene="mxiM"
                     /locus_tag="K0W65_002129"
     CDS             19728..20135
                     /gene="mxiM"
                     /locus_tag="K0W65_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HBI3095391.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20119..20547
                     /gene="mxiM"
                     /locus_tag="K0W65_002130"
     CDS             20119..20547
                     /gene="mxiM"
                     /locus_tag="K0W65_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HBI3095392.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20677..21309
                     /gene="mxiE"
                     /locus_tag="K0W65_002131"
     CDS             20677..21309
                     /gene="mxiE"
                     /locus_tag="K0W65_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HBI3095393.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21296..22996
                     /gene="mxiD"
                     /locus_tag="K0W65_002132"
     CDS             21296..22996
                     /gene="mxiD"
                     /locus_tag="K0W65_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HBI3095394.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            23015..24082
                     /gene="mxiC"
                     /locus_tag="K0W65_002133"
     CDS             23015..24082
                     /gene="mxiC"
                     /locus_tag="K0W65_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HBI3095395.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24095..26155
                     /gene="mxiA"
                     /locus_tag="K0W65_002134"
                     /gene_synonym="virH"
     CDS             24095..26155
                     /gene="mxiA"
                     /locus_tag="K0W65_002134"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HBI3095396.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26168..26569
                     /gene="spa15"
                     /locus_tag="K0W65_002135"
                     /gene_synonym="spaK"
     CDS             26168..26569
                     /gene="spa15"
                     /locus_tag="K0W65_002135"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HBI3095397.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            26573..27865
                     /gene="spaL"
                     /locus_tag="K0W65_002136"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26573..27865
                     /gene="spaL"
                     /locus_tag="K0W65_002136"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HBI3095398.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            27958..28296
                     /gene="spa13"
                     /locus_tag="K0W65_002137"
                     /gene_synonym="spaM"
     CDS             27958..28296
                     /gene="spa13"
                     /locus_tag="K0W65_002137"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HBI3095399.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28283..29161
                     /gene="spa32"
                     /locus_tag="K0W65_002138"
     CDS             28283..29161
                     /gene="spa32"
                     /locus_tag="K0W65_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HBI3095400.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29155..30036
                     /gene="spa33"
                     /locus_tag="K0W65_002139"
                     /gene_synonym="spaO"
     CDS             29155..30036
                     /gene="spa33"
                     /locus_tag="K0W65_002139"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HBI3095401.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            30041..30691
                     /gene="spa24"
                     /locus_tag="K0W65_002140"
                     /gene_synonym="spaP"
     CDS             30041..30691
                     /gene="spa24"
                     /locus_tag="K0W65_002140"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HBI3095402.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30706..30966
                     /gene="spaQ"
                     /locus_tag="K0W65_002141"
     CDS             30706..30966
                     /gene="spaQ"
                     /locus_tag="K0W65_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HBI3095403.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30966..31736
                     /gene="spa29"
                     /locus_tag="K0W65_002142"
                     /gene_synonym="spaR"
     CDS             30966..31736
                     /gene="spa29"
                     /locus_tag="K0W65_002142"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HBI3095404.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31743..32771
                     /gene="spa40"
                     /locus_tag="K0W65_002143"
                     /gene_synonym="spaS"
     CDS             31743..32771
                     /gene="spa40"
                     /locus_tag="K0W65_002143"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HBI3095405.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32784..33026
                     /locus_tag="K0W65_002144"
     CDS             32784..33026
                     /locus_tag="K0W65_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095406.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
     gene            complement(33633..34141)
                     /locus_tag="K0W65_002145"
                     /pseudo
     CDS             complement(33633..34141)
                     /locus_tag="K0W65_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        10594 a         5034 c         6865 g        11648 t
ORIGIN      
        1 ccggctactc attccctgct tgtgcaggat tttaatttcc ataactgtct caaaagtgac
       61 cataagctct cctgaatcag gagagcagat taccccctgg atctgatttc aggcgttggg
      121 tgtggatcac tattgcaccg ttcgtgacac ttgttgacta cctgtgcggc atctggtatc
      181 aaggtggcgt gaagccggtt caggttctca tgcggaacgt tggaacctat atccccccga
      241 taccaaaggc gaaatgtaag tgacaaaatg caatgaacac gttctccgtt aactgaggag
      301 catggtgata aataaaggta aatgcagaag gtgtagagtc tcgcagattt ctgacatcat
      361 agagcctaac attagatgca taacgctaca gattgtagaa tctctacagg tgaaatatac
      421 actggttggc gtttatgtcg caaaacatgt cattcaggtt catctcacca ataaggatat
      481 gagtgaagtg gaggataagt gagtctgctg tcagagtttt ctggtgtatg tcagtaactg
      541 cgaaccatgt ctgcttgata tgggagctta tggaggctct ctgtacaagg ttagggagct
      601 aacaaagcag ggataaaaag tccgcctgat gcaggcacgg ttcgttaaat cttttgccat
      661 tggtaacaaa atggtgcgat gaatgttcag gctatcttta tcttgatggt ggttcagctg
      721 tttggtaaag aaatcgctgt aacaatagaa gaactgcagg cagtactggt cccacatcgt
      781 ttgctcagtc aactggtgaa gttccgtagc gcgtaaatta atgctttgca tgaaacgtta
      841 ctggagtgga tgaaagaata cctgcatata tcattactgc gttggattac cagtacaaca
      901 gattgaatga actggacatg ttgatagacg gcattgaaaa gcagcttata agcatagtgc
      961 ggcagagtta attggagagc acttcgcctt ctgagtatta agtaagcatg gtggtacctg
     1021 cggatactat ctattcactg tgctcggtcg gcagccttac tgaccaagga gttgggggag
     1081 ttgataacaa agttgaaaaa acagctctca accaaggtgg ttattgtaac aatagcaaat
     1141 aagctggtgt gacctgtatc acaataaccg attatggacg taaatacgac aaaaatatgt
     1201 cagtatcagg cggtattaat tgctgatgct gatggacatt aactcttaat aaaagggtgg
     1261 atgctgaaag gttgttatgg tgacggacgt gatgacgacg gaaaagagta tgaggttttg
     1321 gatttaaagt tcgttcgggg aataaggacc cggtcagcta attctcatag tagttcgcat
     1381 tgctggatgc aatactgtgc tgcaattact cgtcgatgtc ggaacaacgg aagccttgca
     1441 aacggggatg tattcataca ggtgttatgt tatatggggt tttattgcag ggggctatac
     1501 caagagaata tatgatatct catcagactg atgtgagggt aaatgagaat agagtaaacg
     1561 aacaaggttg ttttttggcg aggaagcaga tgtatgacaa ttcttgtgga gctgccagtt
     1621 tactctgtgc tgcaaaggaa ttaggggttg ataaaatacc acaatacaaa gggtctatgt
     1681 ccgaaatgac aaggaagagt tctcttgatt tggataatcg ttgtgagcgg gatttatact
     1741 taataactag tggtaattat aatccgagaa tacacaaaga taatatcgcg gatgccggtt
     1801 attctatgcc tgataagata gtcatggcta cgaggcttct tggattgaat gcttatgtcg
     1861 ttgaagaaag taacattttt tcgcaagtaa ttagttttat ttatcccgat gcaagggact
     1921 tattaattgg catgggatgt aatatagtac atcaacgaga tgtgttatct tctaaccaaa
     1981 gagttttgga agctgttgct gtatccttta ttggagttcc tgtcggcctc cactgggtgt
     2041 tgtgtagacc tgatggaagc tatatggatc cagctgtagg agaaaattat tcttgttttt
     2101 caactatgga attaggtgca agaagaagta attctaactt tataggatat actaaaatag
     2161 gtatttccat agttataact aatgaggctt tgtaaattgt cgttttttat ctgtgaagga
     2221 gcttgaatat tttggggggg cataacaaat gatattactg agtacaaatt tagctatatt
     2281 gggcggatta gttgtgtggg aagacgaaat gtttctcttg aaataagtga actccctcaa
     2341 ggaatgattg tgtgcttaaa tatttaagtg ctggtaagtt tgagaaggct gacatatttt
     2401 gcagagcaaa cagtgattga cttggatgat atttttattt tagtcactcg ttgtaattgg
     2461 agtatgcttt attttatttg aaaatagatg tattttatac ccgcaattta aactgtaagc
     2521 cctatgaggg gtatggatat tataagttac tctttgacca agcataactt ttggggttag
     2581 aaaaataaat ctcttgttaa taattaatat gattttaaaa tcaaacttgt ttataattat
     2641 aaaataatgt aagtcggtca ttttatggga agattaggag aataaaacag taattatatt
     2701 tgagaaatct gctaatatat tcttaaagct tttttgatac tgtgaacgaa gtctgttaat
     2761 ggtggatttt ttgtttatca ccatgtaaaa aaaattgagt agtaaaagcg ttgactgcta
     2821 atcctcgctg tttttattga aacgattaaa aatacgctga atattttcgg ccagtcactc
     2881 atattcacat aaaattttga ccatcatgcc gcatccctta gttgcacaat tcattttcaa
     2941 tgatatgaat attactaatt tcaccagata attttctcgt ggtcagcagg gtatgagttt
     3001 tcaccgtaat tttaaggaaa cacagtgcgt ggggattgaa taacagatag ccgacggaat
     3061 taccccaccg gctgaaagtc aagttcaacg aagattttta ttatctgaat tgggcagttt
     3121 acatcagtgt tcgatgttta tgaagacgat agatggcgag aaattatatc ccgaatagct
     3181 tcatctattt ctctctgaac tgttttgttt attttagata atgtaaatat tagagttctg
     3241 tttgttttat tctcttttct ttttatgtaa gtccgtttgt ctttagaaat aaataacgag
     3301 tcaactttta gcgaagggtt ttgctttctg tttttgcttt tctttatgat gtttaaaatt
     3361 tcttttttat atatgtccgg gggcaaattt gtatcaaggt cttttatttc ttcctttaat
     3421 attggtagtg tagaactaag agattcatta gccttttcaa gtcctttata ataattgaat
     3481 aatatcttgt agtcattaaa gttaagttct gacgcgattg gaaatagaga tattatttcc
     3541 tgtggaacgc ttgctgcctg aaaggcacga gtgactttcg cgcgagacag attctctttt
     3601 ttggctatgt ctttatagga catccctgat actttcagaa aattaagacc aataccaagt
     3661 tctcggatgc tatgctcttt tgctgtttgt atatcgtttg ctagttttct ggcatcaaga
     3721 gtagatatat attcttttga atatagaact tcaagatctg ctcctgcata tattgcagat
     3781 gctcttctac gagtgccatc cagaatttca attctaccat caatctccct tcctattaca
     3841 gggaagaatt gttgtagctt tatagttttt atgatatcgg ctaatgattc ttctgttagg
     3901 gattcttgat cgcgtacatt cgttttttgg ttgacgaagg ttaaatcttg gatcctttta
     3961 tgaggaatct tggctttgat aaaggaaact ttattaccag aatggagtgt aaactctttc
     4021 ttttggcctt cctttatact taacaagtcg ttgcacaaat ccaccatatc acaccctgtt
     4081 tattcatatt gatttatatt gacaaaagtt aaatgcagtg tgagcaagat tgtatgtaac
     4141 tgttgatgat agtcaacggt agtcttaaaa attactattt taatagctgg attgatgcaa
     4201 tttatcttta ttggataaaa gaataatatt cttgtggagc tctgatgtga gagctccata
     4261 gatgaaaaaa tgcttatcaa ttactatatt gattttgtac tatttttgac tacagaaata
     4321 agatctccaa tagtaataat gtgttctaat gtagattcat ctatacgtag attgaactca
     4381 aaactaatca tatccagcat ttcaatcaga gaaagagaat cggcagaaag atcttcaata
     4441 aatttagttt cttcagatag tttgctgtac gctataccat agcaaaaggc cactattgat
     4501 aatatttttt cttttatcat attttaatcc ttattgatat tctttaatac ttttgatagg
     4561 gaagtggtta catcttttgc tgctttatat atattattgt tttcagctgt aatatcattg
     4621 ttatttttta aatcagatat atcatctgta agtaattctg cagaggtatc gtcaattttt
     4681 gacaatacct tgctgagggc gctagatact tcttttgcct tttcatagat cgcatgattt
     4741 ttatcaattg tatctgcgtt ggtttctgtt acgtagtttc ttatatttgc atctgtaact
     4801 tttaatttgt tgtttattct atgttctgtt gtggattttg taatgtcctt tgatttttcc
     4861 tgtaactctc gttcttgtgt tcctgattta gttccaatga gagatataag attacttagg
     4921 gagtttgtta ctttttttga agcttcaaat atcgtttccc gtgttaaata tgtgtctcca
     4981 tttcttgata aaaaatcagt ctgaagtact tttgaatcag tgtttgagtt gaatttattg
     5041 atttgcgaag ctactctatg agaaagagaa gcgctacttt tagttgtatc tgatgagtta
     5101 tcattactgc ttgtcactcc ttccgggtgg agaggggggg tggttgaaga gttctgtatg
     5161 ttcagaggcc tgtgtccccg agaaagagag ttatttgcag atctttcttg actactcgta
     5221 gttaacaggt ctgaacctat tccatccgaa ttatctggag tatggttgaa agccttttct
     5281 aaagctgaat tatttgcaaa tgtctggaca gtatctgata tagagtgtgg tagaacggaa
     5341 gttacttttt gaactagtgc tctttgtgga tttagtaatg agtttccgtt acgggataat
     5401 agatcgtttg tctgagaagt aggagactgt gcatcatttt ctgggttctc gtgatagcta
     5461 ttgtcatata ctctgttgtc aaaaactcta ttatcgtaag ttctattatc attatttata
     5521 tgatagtgaa ttacaggttg ggatgttttc tcattggctc cgggggttgg tccgccatct
     5581 ggcatatctt cgggaggtgt agggaggcca tcttggatag gatgcgaatc ttttatatct
     5641 tctaaaatat cattaaggac ttttctcaac agctcaggaa acacggctac tgtatttaag
     5701 tctcttgaca gtttacctat accaaatcct ttactatcaa tagtattttt acgttgattg
     5761 cgcaaactat caaaggcaag ttttgcctgt gcgtcaacta aatcatctag tttgagagat
     5821 gaaacagttt cattacttaa ctttaattca gcaagaatat tattcttgat aaatgatgat
     5881 acttcagtat tgaggagttt ttgtcccaat gataaagctt caccagatgt gtgttgttgt
     5941 gctaatagtg caagtctgtg ctcaatgttt tgtcgtaatt gatggagttt cttattcttt
     6001 gccgaccatg gtccaaaggc agaatcttgg gataatttat cttttataat tagttccgaa
     6061 tactgtgcga caattttttg aagtcccttt tcgttttcaa aattaagacc attttgatgt
     6121 actaaggagt acaaaacatt tgaaaattcg agaatctcgc ttttggcata cgcattcatt
     6181 gagttggaat aaatgcttct taacacatct tctttccctg aggaagaaaa taaaaaatca
     6241 agacatttct gattaaaaga cgttgcaaag ttttcttttt cagacactga tgctggtgtt
     6301 tttagagaag tttgcgaact atggatatcg gatattttgc tttttaactc gctgtattct
     6361 gttgatgatg gtgaagttgc cttatataag aatgttggcg cttgagtatt atttacatta
     6421 tgcatggcgc acctcagaaa tggagaaaaa gtttatctgt atctgtacat gagcttattg
     6481 tactactcaa aacctttact aaattatcaa aaatactatt ggcattactg tatttttgaa
     6541 ctaaagtttg aagattattt ttcattgttt catcttcggc agagaatccg gcattccatg
     6601 cctgatattt tgcattatct agcacaacct cgccatttcc acctagatta tctaagcttt
     6661 ttaacatatt gtctattggg gtcatgttta tactgacaac atatcccccg tttttttgag
     6721 ataccttgcc gattgttcca cctaattctg taagccattt atttgcttgt tcctgactaa
     6781 cagtattatt tgctggatat agcggtttat ctttatattt ttccttgagt tcttccaatg
     6841 cctttttaag cgagttgact tgtaatttca cggagtttcc gtcgttacct ccgggagaga
     6901 tccagccggc aagactggaa agaacagcgc taaaatcttg atacatttga gtatatgaac
     6961 taacggcatg ttcatatact ttcagatact gttcattaat atcattgatg gagtttgcaa
     7021 ttttagccca cagttctcta tgagatatca tttgatctcc atcaagctcg gcttctttcg
     7081 gagctgaatg taataattct cttgcgtctt tattaattgg atattcgtgc ctggaaagaa
     7141 tatctaatag ttgagcggat ttatttacat ccatgctaat ctgagatgaa tgtaatgcta
     7201 tttcttctag cgatgtttta gtcaacgttt tttgtgaaag ctctttcttt aatgcctgat
     7261 ttgttgttct gatattatga agggtgtcgt tgagcatagt aagggaactt acaggatgag
     7321 aactggtcgt tgtttttata tcagaattaa ctgtttcggt tgatgaaccg ttggtattgt
     7381 ttggactgaa tgatgaggtg gaaatactat tagtcagagt tgttatattc atggttattt
     7441 ccttacagga agagccatat attattatgg ctcttggata tattaagctc gaatgttacc
     7501 agcaatctga ctggctgtcg aattctttga ttggttgatg ctgtcaatta tattcaataa
     7561 tttttgtatt aattgatttg tcgcttggga tgcttcttta gatacttggg atgcttcctc
     7621 tgcttgttta ctgctggcct gactgattag ttgttcttct tcttcaagag cagatgcata
     7681 acgccctcct gatgtggata tattaccagc aacggctgat gtttccattg ttgcacggcc
     7741 aatgttttgt ttttgctgcg cagaaagggt attgagctcg taagttcttc tctgggtgtc
     7801 aattttatcc tgcaaagaaa tatcgggaga actaagagaa gttttatgtt cagttgacag
     7861 ggatatatta tctggcgcca gtttattttt acctaaaaat tttgtgctag agttagtttg
     7921 tggtgaggtg atatttgtat ctatttgttt atttaaccct aatttagaac ctgcaagctc
     7981 tttttcaaga gattgagcag tggcaaggtt ctttcttaag gctccttttt ggtcgctaat
     8041 ccctgaatgc gtttttttgg caccgatacc cgttatacct acttgtgtga ctgctccagt
     8101 aatgcttgat gatagggctg ccaggccttg ccgaacaatg ttctctgcag ctgattttgt
     8161 agcatcgaac gcaatcaatg acaattgaga gcccaattta gtttctgcag tgcggagagc
     8221 agaaagtaga acggctacac taataattaa agaaacagca ttagaagaaa gactggaaat
     8281 atccagagtg ttctctggag taagggtgtg actcatctct gaaagatctt tcatcttttt
     8341 gtcagtaagg cgagcaatga tttctgaatc atgctgaacc tgctctgata actttaaaag
     8401 ttgatcatca tttaatgtgg ttgttaatac gggattttta ccgacattaa gtggaatatg
     8461 cgctgcaatc tgctgataat tttgtgattt ttgtgtttcg gaagaacttt gagtttgttt
     8521 tgtggatata tctgtatata aaatctgggt tggttttgtg ttttgaattt ccataacatt
     8581 ctccttattt gtatcaagca gtagtttgtt gcaaaattgc ttttgcaaca tcagttctat
     8641 tagcctgaga gttggacatt gaggctaata gatctgcaat tacttcctgc aattggccga
     8701 atttttctat tgcttcactg atatattttg acagttgttc aacttgatat ttcgataatg
     8761 tcaggtctgc tagatttgtc gacgcgctgt tctggaaaac agcagaagcg acacttcctc
     8821 ccgcttgtgt ggcagagtta acactttctc ctaataaaac tgcttggttt aaatgggtgg
     8881 aaataatttg tttggatatt acttcatcac ccgctgcacc aagaaattta tttaatctgg
     8941 caacagcatt agtgattaaa tcgtccagtt gagaagaaaa attcttgaga aactttggta
     9001 taaggtctgt gagggtttta cctattattt tgccaatatt ttctgcaagt tttgctgctg
     9061 cctgtttacc aacagtggct acgagaacga ctgctgcaac taggacaaga gcgcctgcga
     9121 ttgcccccag aatagagcca atcattttgg cttttttcga gtcgacgccc aagccttcga
     9181 gcatttttgt aaatgcatct gaaaggagtt tgattaaggg ttcaatgact gctttcatga
     9241 tcggattcag ggcttgttcc atgaaggaat tgccggtcgc tgcttgtact atagcatccg
     9301 taaccataag agctaaacca acagctgcca gtgctagaga ggctcctcca gaaaaagctg
     9361 ctgcaacaac actaacgata gttaaaagtg ccccaagtat tttcccaaca caacccatta
     9421 ctctgttgag ttcttctgct ttacgtactt cagcagcata ctcatcagat tttctctcca
     9481 tttcagtttt tcttgattct tggagagact ggaatagagc cagatcattt tttaaagatt
     9541 cttcattatt ttttccaact agttgaataa aggttgccat caattgagta acactggcaa
     9601 gtccggttaa tgatttctgc tgggttgata gctgttcagc agatgctgtg tttgaaaatg
     9661 cagaaaaaga gtctatttct ttttcgagtt gcattgattt atctgtaagt ttgctatgaa
     9721 ttgacagggt tttctgctca atcaatgtcc tgtctttaac tgctgcgtct tttttgatag
     9781 tgagttgtat ttcttcccgg cttaattttt tcttttctgg tgactctggg tcgagttcgg
     9841 ataatcttgt ttgaatttgg ttaattttat tttctaggtc ttttatttta gaatctgcgt
     9901 tttttagttt attaatttgt ttttcatagt ctctggttag tccttcagtt tcagatagaa
     9961 gagtgttaat tttatcggag aattctaggt ttttttgctg tcttgcctgt tgctgggact
    10021 tccaagcagt aattttattt gttaatgcag ttaaagattt ttcaccgagt atttgaataa
    10081 ggtttccaat taaaagcgtt agttgggaac ttgcatttaa tgactttggt gctttaagtt
    10141 cagggattaa tatatttgaa gttgagtgtg cattagttgt attaatattt tggttagcag
    10201 taagatcagc aattgtaaga gaaaataatt tgttagctgc atcatttgca gcttggatag
    10261 tattgtctcc aagctcagtg gaagccaata ttttggcaag aggaaaacca gtggttgtgg
    10321 tgcttacatt atgcataata attactcctt gatatcctga attgcgtcca agtatgattg
    10381 tgcttttatt tttaattttt catcattgct gtgttgaatt acgagttcga agcactcttt
    10441 agcttttaag ggggctttca accgaagctg gcattgtcca gtatggaata ctggtgtata
    10501 gtcatttttt cctaatgcaa aagcgacagc ataaaggtct gctgcttgtt ggaactgttc
    10561 ttttatctga taaatagctg cgagtcccat aatgtagtct acattgtaaa agtcgtatat
    10621 acataaaaac ctgaagaaaa cttcagcttc ctctattctt cctttgttgt aaaagtcata
    10681 agcatatgaa taaatgtcat ccatcatatc atcaggaatt gcattaatat ctttcagtgt
    10741 agcgccagag ttaattgcat caattactgc agtagagatg ctttcatttt cggtgatatt
    10801 taaagacata aggtctcctt tttgtctgct ttactttaat aagtataaga tttaatataa
    10861 aagatttaat ttgtattgct ttgacggtat acagccttta gaacctctgc gcttatttct
    10921 atttgtaaaa cttttgttga ttcattaaat attttatcgt agacggaact ggatactaga
    10981 tttgtaagat ccttcatttg tgtgttaaac aaatccatgt cactgctata gcgttttaaa
    11041 atctttgctc tgaggccaga tgaggcaatc tcgtttagtg ctgtatgact gatagatgtt
    11101 tgtgctgatt tttcatcaag ttttatatcc gggaattttt tttcaatagc tgcaaatatg
    11161 ttttttttgt tatctgaggt caaaacaact ccattgtgta tggccatttc atctatactt
    11221 ctgtttatca ttgaagagac ataagtggtt cgctcttggc tcatccaaca aaatagttga
    11281 ttccccgaat tactatcttt tattttgtct atgacgctgt ccctgatttt atttatattc
    11341 accgggcatc caaaattctt ctgaggaaag cgttgtttta agttaaaaac agataatatt
    11401 ttctttacaa gcttattgcg acttaacgaa tcaaatgatg ggcgaggaat cgcgaattca
    11461 acctgtggaa gtattttgtt tagaatttgc atgatacccc ctatatgtta gttcacttct
    11521 gaagtgatgt ttgcaatggt tagtttaata caaaataaaa attcatacaa agattcaatg
    11581 agttcatcag gtgaaagata ttttttgtag tgtttcttgg tcaaaacaca ttgatgagta
    11641 tcagggagaa gacagataga agaaccataa tctctactca aagagtagtt aaggatgagt
    11701 atgtgttctg gattaataat attttctgta aaaagttcat gttcattaag aagtccggtc
    11761 aaaaagatgt attcatcatt atctgttata caaacaggta tttgttcttc gagcaatata
    11821 aaggcttgat ttttatcgtt aaaatcaagc tttgcacctg ttatttcata aagtttatca
    11881 tatagtttgc gacacatttc tatggccttc tatatattag aatgagagtt attcaataaa
    11941 agcttattga tctgtaataa gtcgtgaatg taaagtttgg ttccaagatc tggcgattta
    12001 agagaattgt aataatcaat aatttttatt gatttttcga tgatttcttc ttttttatct
    12061 gttgcttcgg tttttaattc gtttaataca cgagtcatat tgctgggtgc tgattttact
    12121 aagtatgagt ggcaagcgtt gatgaccgca tcaaagtctt ctgaagatac attttcagta
    12181 ggatgtttgt aaagtgaaat tgataaaggc ttgaatgttt ttggtgtgtg gataaggtta
    12241 attttattga aacttttaag atagctgcgt cgtaattgag tcaatctgga aagatcaaaa
    12301 tcagattcaa tatacttcat tatttcgtta taagactgat tgagactttc aatgcgttgc
    12361 ctaactttat ttgcatacgc gtatacatcg ttaggtgtag caaccaattt aacgtcaggg
    12421 agtggaaagt atatttcaga atttcctgct ttaagaacat taagtgccat accagcacag
    12481 ttttcagatt tactgattaa tttataattt gccaattgtc tgaaagcatc tgctttcttt
    12541 ttgcatataa attttgacat gtttgtttca tcaaggccaa aaaggttatg gctgatttta
    12601 ccatcctttg ttttattatc acctgataat ggaatgtata ctttatcagc actgaccccc
    12661 caataaccat aggggtcttt tttttgatta gctctgggaa aaaatatttt tttgttgtta
    12721 ttttgatctt gttttgaatt taataagttt ctaacctctt ggcctgcatc aagtctttcg
    12781 gctgtttggt ctgaaatcat atttagtttg tcttgcttat aggtatcgac actgtaacct
    12841 gagcgttttt caaaaaggta gtttatttct gtatcttttg taacattccc gccgggatac
    12901 cagctgagat aggtatgagt tggttcctgt agtaaattgt tttttatact gatactagta
    12961 tgccctaaat gggttatgcc tctatttccc cagcaacgaa aaactaaatg tctttcaaga
    13021 tttttggctg taccgatgcc atgaaaacgc tctgcggtat tcaacattaa tttgatgtct
    13081 ctacctgtga aagagtgttg aattgagttt ttagctgctt cataggcggc aaacgcactt
    13141 tctgcagatt tttcgctagc taatgtattg aggaaaagca ataaggattt ttgattctct
    13201 agtcgataat tttttagttg ttcatttaat atctgtgaat ttatctttgc attaatagtg
    13261 cttaatgttc tgaaaaagag atcctttagg ttgaatttct gtgctatcaa tataggtcca
    13321 tgctctgtat cttcaacgcg tataggccct tttgtattgc ttgcgtcaat gaaattgcta
    13381 attttgagga tcatacttta ttaactctcc attacttggt gattgtgggg atgtaaaatg
    13441 tatttggtat ataatatatt caattaataa atttagaaac ttgagcctgt taacataatc
    13501 aaattttctt ttgctgtaca taatatgtac ctcgtgagca tatgtagtgc tcgtttcatc
    13561 atgaaatccc acaagataaa gtgcctgatg tatcaggctc ggagtgttat agaaaaagag
    13621 agaaccctgt tgaataagta tcttttttgt tgtaaaacga ggtacaaaac tgcaatcaat
    13681 gccccattta tgactgagga taattaaatg cacataacta atttgggatt gcatcaggtt
    13741 tcatttcaaa gcggagattc ctataaaggc gcagaagaaa ccggaaagca caaaggtgta
    13801 agcgtgattt catatcaaag agttaaaaat ggagaaagaa ataaggggat cgaagcgctt
    13861 aatcgattat atttacagaa ccaaacaagc ttaacaggaa aaagcctttt gtttgcgcgt
    13921 gacagagctg gagttttttg cgaggcaata aagttggcag gtggtgatac gtcaaaaatt
    13981 aaagccatga tggaacgatt agatacctat aaacttggtg aagttaataa aagacatatt
    14041 aatgagctta ataaagtaat aagtgaagag ataagagcac agctaggcat taaaaataaa
    14101 aaggaattgc aaacgaaaat aaaacaaata tttactgatt atttaaataa taagaattgg
    14161 ggaccggtcg acaagaatat tagtcatcat gggaaaaatt atggttttca attaactcct
    14221 gcctctcata tgaagatagg gaataaaaat atatttgtca aagagtataa tggaaaagga
    14281 atttgttgtg cttctacgag agagagtgat catatcgcga atatgtggct ttcaaaagtg
    14341 gtggatgatg aggggaagga aattttttcg ggtatcagac atggagttat atcagcctat
    14401 ggattgaaaa aaaactcatc tgaaagagct gttgctgctc gtaataaagc tgaggagtta
    14461 gtaagcgcag cattatatag cagaccagag ttattatcac aggctttgtc tggtaaaaca
    14521 gtagatttaa agattgtttc gacttctctt ctgacgccga ccagtttaac cgggggagag
    14581 gagagtatgt taaaagatca ggtgaacgca ttaaaagggt taaactctaa aagggggaag
    14641 ccaacgaaat tattaattcg gaatagtgat ggtcttctga aagaagtaag cgttaatcta
    14701 aaagtggtga catttaactt tggtgtaaat gaattagcgc taaaaatggg cttaggctgg
    14761 aggaatgttg acaagcttaa tgatgaatca atatgttctt tgttggggga taatttcctc
    14821 aaaaatggtg tgattggagg ttgggctgca gaagcaatag aaaaaaatcc accatgtaag
    14881 aatgatgtga tatatctggc taaccagata aaagagattg taaccaaaaa gttacaaaaa
    14941 aatgataatg gagagccgta taaattgtca caaagaatga ctctattggc ttatactata
    15001 ggtgctgtac cttgctggaa ttgtaagagt gggaaggaca gaacaggtat gcaggatgct
    15061 gaaattaaaa gagaaataat aagaaaacat gaaacaggtc agttttctca attaaatagt
    15121 aaattatcct cagaagagaa aagattattt tctactattc taatgaatag tggtaatatg
    15181 gaaatccaag agatgaatac tggtgtgccc ggaaataaag tcatgaaaaa attgccgcta
    15241 tcttcgttag agctatctta ttctgaaaga ataggggacc caaaaatatg gaatatggtg
    15301 aaagggtatt cgtcatttgt ataagaggaa tatatggaag atttagcaga tgttatttgc
    15361 cgagccttgg gtatcccttt aattgatatc gatgatcaag caatcatgct tgatgatgat
    15421 gtgctaatat atattgaaaa agaaggagat tcaataaatt tattatgccc tttttgtgca
    15481 ttgcctgaaa atataaatga tcttatatat gcgttgagcc taaattactc agaaaagata
    15541 tgcttagcta ctgatgatga aggcggaaat ctaattgcgc gattagatct taccgggata
    15601 aatgaattcg aggatgtata tgttaataca gaatattata tttcgcgtgt gcgttggctg
    15661 aaggatgaat ttgcgcgaag aatgaagggg tattaatgtc ccgtttcgta tttattttgc
    15721 tgtgttttat cccatattta gggagagctg attgttggga taaggctggt gaaaggtaca
    15781 atattccatc aagtttgtta aaagcgattg cggaaaaaga atccggattt aataaatctg
    15841 cagtcaatgt taataacaat ggaagtaaag attatggtat aatgcaaata aatgactttc
    15901 attctaaaag acttagagaa atgggatatt ctgaggaaat gctaattagt catccatgtc
    15961 tttctgtaca ttatgcagca aagttattaa atgagtttat gatgatgtat ggaagagggt
    16021 gggaggctgt tggagcctat aatgcaggaa cgtctccgaa aaagaaaaaa gaaagattaa
    16081 agtacgcaga ggatatctat agacgctatt tgcggatagc agcagagagc aagcagaata
    16141 atcgaaggat ataagaggat ttatgtctga ggcaaagaac tcaaatcttg caccattcag
    16201 gttattagtg aaactcacaa acggtgtcgg ggatgagttt ccgttatatt atgggaataa
    16261 cttaattgtt ctcggacgga cgatagaaac acttgagttt ggaaacgaca atttcccaga
    16321 aaatattatt cctgtaacag atagtaaatc ggatggtata atttatttga ccataagcaa
    16381 agataatatc tgtcaattta gtgatgaaaa aggtgagcaa attgatatca attcacaatt
    16441 taattcattt gaatatgacg ggatttcttt tcaccttaaa aatatgaggg aggataaatc
    16501 gagaggccat atactaaacg gaatgtataa gaaccactcg gtgttttttt tctttgctgt
    16561 tattgttgtg ttaattataa ttttttccct ctctttaaag aaagatgaag taaaagaaat
    16621 tgctgaaatt atagatgata agagatatgg aatagtcaac actggacagt gcaattatat
    16681 tcttgccgaa acacaaaatg atgcggtatg ggcgtctgta gccttaaata aaaccggatt
    16741 taccaaatgc agatatattc tggtgtcaaa caaagaaata aatagaattc aacaatatat
    16801 caatcaacga ttcccattta tcaatttgta tgtgttgaat ttagtgagtg ataaagcgga
    16861 attactggtg tttttaagta aagaaagaaa tagttcaaaa gataccgaac ttgataaatt
    16921 gaaaaatgct ctgattgttg aatttccgta tatcaaaaat attaaattca actatcttag
    16981 cgatcataat gcaagggggg atgctaaagg tattttcaca aaggtaaatg tccagtataa
    17041 agagatttgt gaaaataata aagttacata ttctgtcaga gaagaactga cagatgaaaa
    17101 gcttgagctc attaatagac tgatttctga acataaaaat atatatggag atcagtatat
    17161 tgagttttct gtacttttga tagatgatga ttttaaaggt aaatcatatc ttaacagcaa
    17221 agacagttat gtaatgttga atgataaaca ctggtttttt ttagataaaa ataagtgagg
    17281 ataaaatgag tgttacagta ccgaatgatg attggacatt gagttcatta tctgaaactt
    17341 ttgatgatgg aactcaaaca ttacaaggtg aactaacatt ggcactagat aaattagcta
    17401 aaaatccttc gaatccacag ttgctggctg aataccaaag taaattatct gaatatacat
    17461 tatataggaa cgcgcaatcc aatacagtga aagtgattaa ggatgttgat gctgcaatta
    17521 ttcaaaactt cagataatag ggagcattca tgaattacat ttatccagtc aatcaggttg
    17581 atattatcaa agccagtgat tttcaatctc aagagatatc aagtctggaa gacgtcgtgt
    17641 cggctaaata tagtgatatt aagatggata cagatattca agtatcacaa ataatggaga
    17701 tggtaagcaa tccagaatca ttaaacccag aatctttggc caagttacag acgacgctct
    17761 caaattattc aataggagta tcattagctg gcacgttagc aagaaaaaca gtttcggctg
    17821 ttgaaacttt attaaagtct taatttatat gattaggtat aaaggtttta ttttattctt
    17881 gttgctgatg ttgattggat gtgagcaacg tgaagagtta atttctaatt tatctcaaag
    17941 acaggcaaat gaaataatat ctgtgctaga acgccataat attactgcta gaaaagttga
    18001 tggaggtaaa caggggatct cggtacaagt cgaaaagggg acatttgcat cggcagttga
    18061 tttgatgcgc atgtacgatt tgccaaatcc ggagagagtt gatatctcac aaatgtttcc
    18121 tacagattca ttagtgtctt ctccaagagc tgaaaaggcc cgtttatata gtgctattga
    18181 gcaacggctg gaacagtctt tagtttctat tggtggtgtt atttcggcaa aaatacatgt
    18241 tagctatgat cttgaagaaa aaaatatatc ttcaaaaccg atgcatatat cagtaatcgc
    18301 tatatatgac tcaccgaaag agtctgaact attagttagt aatattaagc gatttttgaa
    18361 aaacaccttt tctgatgtta agtatgaaaa tatatctgtc atattaactc cgaaagaaga
    18421 atatgtttat acaaatgtac aacctgttaa ggaagttaaa tcggaatttt taacaaatga
    18481 agtaatatat ttatttctcg ggatggctgt actagttgtc attcttttgg tatgggcatt
    18541 caaaacaggg tggttcaaga gaaacaaaat atgataagaa tggatggaat ttataaaaaa
    18601 tatctttcaa taatttttga tccagcgttc tatataaata gaaatcggtt gaatttgcct
    18661 tctgaactgt tagaaaatgg cgtaatcaga agtgagatta ataatctcat aattaataaa
    18721 tatgatctaa attgcgatat tgaaccttta agcggggtaa ccgctatgtt tgttgccaac
    18781 tggaatttac ttccagctgt tgcttatttt atcggtagtc aagagtctcg gttaattaat
    18841 catagtgaga tggttattag ttattatggg ggcaaaatca gtaagcaagg ggaggccgct
    18901 attcgaagtg gtttttggca tcttattgcg tggaaggaaa atatatctgt tggtatatat
    18961 gaaagaataa acttattatt taatccaatt gctttggagg gaaattatac gcctgtagaa
    19021 agaaatttgt cccgattaaa tgaaggtatg caatatgcaa aaaggcactt taccggtatc
    19081 cagacatcat gcctatgatg gtgttgttat aaagagaatt gaaaaggaat tatgtaaaac
    19141 cataaaagat agagacactg agagtaagaa gaaagcaatt tgtgttataa aggaggctac
    19201 gaaaaaagct gaatcactgc gtatagatgc tgtatgtgat ggttatcaaa ttggtataca
    19261 gactgctttt gagcatatta ttgactatat ttgtgaatgg aagttaaagc aaaatgaaaa
    19321 tagaagaaat attgaggact atataactag tttgctttca gaaaatttac atgatgagag
    19381 gatcattagc actttattgg agcagtggct tagttcgttg cgtaatacag taacagaatt
    19441 aaaagtggtt ttgcccaagt gtaatttagc actgaggaaa aagttagaac ttgatctcca
    19501 taaatatcgc tcagatgtca agattatttt aaaatattct gaagggaata attatatatt
    19561 ctgtagtggt aatcaagtag tggagtttag tcctcaggat gtaatatcag gtgttaaaat
    19621 agaacttgcg gaaaaattaa caaaaaatga taaaaaatat ttcaaagagt tagcacataa
    19681 gaaactgaga caaattgcag aagacttgtt aaaagagaat cctgttaatg attaatcaaa
    19741 taaatgcaag caatgctctg cagcaaagat taaatagtga agaagttgtt aatttaaatg
    19801 agagattatc atcttcgcaa tcatttgatg aggatataat ctatgaaata atgcaatatt
    19861 tctcacagag tgaattaaat tccatagata atgatgaact gcacaataag attgaacaat
    19921 tattcaattc acgttttcct tatcttacag ctgcacaaaa aagttcactt cttaataaac
    19981 tcatagatgc aaatcaatat gtcgatttgc atgagggatt ttatgcatca ttgtccatat
    20041 ataacaatat tgatttctat attaagacta caaccttcga ttcattaatt agtgtctttg
    20101 aagcagggag agaggcagat gattcgacat ggtagtaata agttgaaaat atttatttta
    20161 agtatattgc tattaacact gagtgggtgt gctttaaagt catcatctaa ttctgaaaaa
    20221 gaatggcata ttgttcctgt aagtaaggat tatttttcta ttccaaatga tttattatgg
    20281 tcgtttaata caaccaataa aagtataaat gtttactcta aatgtattag tggtaaggcg
    20341 gtttatagtt ttaatgcagg taaattcatg ggcaacttta atgttaagga agtagatggg
    20401 tgcttcatgg atgcacaaaa gatagctata gataaactat tttctatgct gaaagacggg
    20461 gttgttttaa aaggtaataa gataaatgat accatcctta tagagaagga tggggaagtt
    20521 aaattaaaat taattcgagg gatataattg tattgtgagt aaatataaag gtctaaatac
    20581 aagtaatatg ttttacattt actctagtgg acatgaacca gttaacgttg agcttgtaaa
    20641 agataaagaa cgtaacataa ttgagctggc tccagcatgg aagggttttt ttttgtccga
    20701 aatcaaaata taaaattcag tgataatgtg aactatcatt atcgctttaa cattaatagt
    20761 tgtgcaaaat ttcttgcttt ttgggactat ttcagtggtg ctctagtaga gcatagccat
    20821 gctgaaaaat gtattcattt ctatcatgag aatgatcttc gggatagttg taataccgaa
    20881 tcgatgcttg ataagttaat gttaagattc atcttttcat ctgaccagaa tgtaagtaat
    20941 gcactggcta tgatacgaat gactgagtca tatcatttgg ttctttatct tctgagaact
    21001 attgagaaag aaaaggaggt aagaataaag agtctgacag agcattatgg tgtgtcggag
    21061 gcttatttta gatctttatg tagaaaagct ttaggggcaa aagtcaaaga acaactgaac
    21121 acttggcggt tagtaaatgg tttattagat gtatttttgc acaatcaaac aatcacttca
    21181 gctgccatga ataatgggta tgcatcaaca tcccattttt caaatgagat aaaaacacga
    21241 ctaggctttt ctgcaagaga attatcgaat attacttttt tagtgaaaaa aataaatgaa
    21301 aaaatttaat attaaatctt tgactctctt gattgtattg ttaccattga tagttaatgc
    21361 taataatatt gactctcatt tattagaaca aaatgacata gcgaaatatg ttgctcaaag
    21421 tgatacggta ggatcttttt ttgaacgttt ttctgcgtta ttaaattatc ctattgttgt
    21481 tagcaaacaa gctgcaaaaa agagaatatc tggggagttt gatttatcta atccagaaga
    21541 aatgcttgaa aaactgacat tacttgtggg actcatatgg tataaagatg gaaatgcatt
    21601 atacatatat gactctggag aattaataag caaagttatt ttgctggaga atatatcact
    21661 taattatctt attcagtatc tgaaggatgc taacctttat gatcacagat atcctataag
    21721 aggaaacatt agtgataaaa cattctatat atctggtcct ccggcattgg ttgaactggt
    21781 agctaataca gcaactttgc ttgataagca ggtttcttct attgggacag ataaagttaa
    21841 ttttggggtt ataaaattaa aaaatacatt tgttagtgac agaacttata atatgagagg
    21901 ggaggatatt gtcatcccag gggtggctac tgttgtagaa cgtttattaa ataatggtaa
    21961 ggctctgagt aacagacaag cccaaaatga tccgatgcca ccctttaata ttacacagaa
    22021 agtatctgag gatagtaatg acttttcatt tagttcagtt accaatagtt ctatattaga
    22081 ggatgtatca cttattgcat atccagaaac caattctata ttagtcaaag gtaatgatca
    22141 gcaaatacag attattcgcg atattatcac tcagttagat atagccaaac gacatataga
    22201 attatcatta tggataatcg atattgataa atctgaatta aataaccttg gtgttaattg
    22261 gcaaggaacg gcttcatttg gtgattcgtt tggtgcgtca tttaatatga gctcatcagc
    22321 atcgataagc actttggacg gcaataagtt tattgcttca gttatggcat taaatcagaa
    22381 aaagaaagca aatgtagttt caaggccggt gattttgaca caagagaata ttccggctat
    22441 atttgataat aacaggactt tttatgtgtc attagttggt gagcgcaatt cttcgttgga
    22501 gcatgttacg tatggaacat tgataaatgt tatacctcgc ttttcatctc ggggacaaat
    22561 tgagatgtct ttaacgatag aggatggaac aggaaatagt cagtcaaatt acaattataa
    22621 taatgaaaat acatctgtcc ttccagaagt tggaaggacc aaaatcagta caatagcgag
    22681 agttccacaa ggcaaaagtt tgttgatagg tggttatacg catgaaacga actctaacga
    22741 aataatatct attccttttc tctcctctat tcctgtaatc ggtaatgtgt tcaagtataa
    22801 aacaagtaat atcagtaata ttgtaagggt ttttctgatt caaccccgtg agataaagga
    22861 gtcaagttat tataatactg cagaatataa aagcctcata tctgaaagag aaatacaaaa
    22921 gactacacaa ataatcccat ctgaaactac tttgctggaa gacgaaaaat cattggtttc
    22981 atacttaaat tactaactat aaagtaggtg atgtatgctt gatgttaaaa atacaggagt
    23041 ttttagctct gcattcattg ataagttgaa tgcaatgaca aattcagatg atggagatga
    23101 gactgctgat gcagagcttg attctggctt ggctaatagc aagtatattg actcatctga
    23161 tgagatggct tccgctcttt cgtcatttat aaacagaaga gaccttgaga aactgaaagg
    23221 aacaaatagt gatagtcagg aacgtatttt agatggggaa gaagatgaaa ttaatcacaa
    23281 gatttttgat ttaaagagaa cgttaaaaga taaccttcct ctagatcggg attttataga
    23341 cagactaaag agatatttta aagatccaag tgatcaagtc ttagcattaa gggaactttt
    23401 gaatgaaaaa gatcttactg ctgaacaagt cgaattatta actaaaatta ttaatgagat
    23461 aatatcaggt agtgaaaaaa gtgttaatgc tggaataaat tcagctatac aggctaaatt
    23521 atttggcaac aaaatgaaac ttgaaccaca gcttttgcgt gcatgttatc gtggttttat
    23581 catggggaac atatcaacaa cagatcagta tatagaatgg cttggtaatt ttggttttaa
    23641 tcacagacat acaattgtga attttgtaga gcagtcactg attgtagaca tggattctga
    23701 gaaaccgagc tgtaatgctt atgagtttgg ttttgtgtta tctaaattaa ttgcaattaa
    23761 gatgattaga acttcagacg taatttttat gaagaaactg gaatcctcaa gcttgctaaa
    23821 agatggcagt ttaagtgcag agcagctatt gctaacttta ttatatattt ttcaatatcc
    23881 aagtgaaagt gagcaaattc ttacttctgt tatagaagta tcacgagcca gtcatgagga
    23941 ttctgtagtg tatcaaacat atctatcttc tgttaatgaa agtcctcatg atatatttaa
    24001 aagtgaaagt gaaagagaaa ttgcgatcaa tattctacga gagcttgtca caagtgcata
    24061 caagaaagag ctttctagat aacaggagat aaaagtgatc cagtcttttc ttaagcaagt
    24121 aagtactaag cctgaattaa ttatattagt gcttatggtt atgatcattg ctatgttgat
    24181 cataccatta cctacatacc ttgttgattt tttaataggg cttaatattg tacttgctat
    24241 tctcgttttt atgggatcat tttatattga aagaatattg agtttttcta cattcccttc
    24301 ggttttgttg ataacgacat tatttcgtct cgctttatca ataagtacta gtcgactaat
    24361 tttagttgat gcggatgcgg gtaaaattat tactacattt ggccagtttg tcattggtga
    24421 tagtctggct gtaggttttg ttattttttc aatagtaact gttgtgcagt tcattgttat
    24481 aacaaaagga tctgaaaggg ttgcggaggt agctgctcgc ttttctcttg acggtatgcc
    24541 ggggaaacaa atgagcattg atgcggattt aaaggctgga attattgatg ccgcaggagc
    24601 taaagaaaga cgcagtattc tggaacgtga gagtcaattg tatggttcat ttgatggagc
    24661 aatgaagttc attaaaggtg atgcaattgc tggcatcatt attatttttg tcaatttaat
    24721 aggaggtatt tctgttggta tgagtcaaca tggaatgtcc ctatctggtg cattatctac
    24781 ttataccata ctaactattg gtgacggatt ggtctctcag attcctgctc tgttaatttc
    24841 tataagtgca ggatttatcg ttacaagagt taatggtgat agcgataata tgggacgtaa
    24901 cataatgtcc cagatatttg ggaatccttt tgttctcatt gttacatcag ctcttgcctt
    24961 ggcgataggg atgttgccag gttttccgtt ttttgttttt ttcctgatag cagttacttt
    25021 gacggcttta ttctattata aaaaggtcgt agaaaaagaa aaaagtctgt ctgagtctga
    25081 ttctagtggc tatactggta catttgatat tgataatagc catgactcat ctttggcaat
    25141 gatagaaaat ctggatgcta ttagttcaga aaccgttcct ttaattttat tatttgccga
    25201 aaataagata aatgcaaatg atatggaagg tcttattgaa aggataagaa gtcagttctt
    25261 tattgattat ggtgtcaggc ttccaactat tttatataga acaagcaatg agcttaaggt
    25321 cgatgatatt gttttgttaa taaatgaggt gcgtgccgat agttttaata tatattttga
    25381 caaagtgtgt attacagatg aaaatggaga tatagatgct ctaggtattc ctgttgtttc
    25441 aacttcatat aatgaacgtg ttatttcttg ggtagacgtt tcatatacag aaaatctaac
    25501 taatattgat gctaaaatta aaagtgctca agatgaattt tatcaccagt tgtcacaagc
    25561 tttattaaac aacataaatg agatttttgg tatacaagaa acaaaaaata tgttagatca
    25621 gtttgaaaat cggtatcctg atctattaaa ggaagtcttc cgacatgtga ctatacagag
    25681 aatttctgag gtattacaaa gattgcttgg agaaaatatt tctgttcgca atttaaaact
    25741 tattatggag tctttggcgc tttgggctcc cagagaaaaa gatgtcataa cattagttga
    25801 acatgtccgt gcatcacttt ctaggtatat ttgtagtaaa atagctgttt ctggtgagat
    25861 taaagttgtg atgctttccg gatatattga ggatgcaata agaaaaggga taaggcaaac
    25921 ctctggtggc tctttcttga atatggatat agaggtttcg gatgaggtaa tggaaacttt
    25981 agcacatgct ttgagagaat tgagaaatgc aaaaaaaaat ttcgttcttt tggtatcagt
    26041 agatatacgt aggtttgtta aaagacttat agataacaga tttaagagta tactcgttat
    26101 atcgtatgct gagattgatg aagcatatac cattaatgta ttaaagacta tttagtgagg
    26161 tttaaatatg agtaacatta atttagttca attagttaga gatagtcttt tcacgattgg
    26221 ttgtcctcca tcaattatta cagatttaga cagccattca gcgattacca tttctttgga
    26281 tagtatgcct gctattaata tagctcttgt caatgagcag gttatgttat gggctaattt
    26341 tgatgcacca agtgatgtaa aacttcagtc atctgcttat aacatattaa atttaatgtt
    26401 aatgaacttt agttatagca ttaatgagct ggtagagctt catcgttctg atgagtactt
    26461 acagttaaga gttgttatta aagatgacta cgttcacgat gggattgttt ttgcagagat
    26521 tctgcatgag ttttatcaga ggatggaaat cttaaatggg gtcttataat caatgagcta
    26581 tacaaaattg ctcactcaat tatcttttcc taatagaatc tcggggccaa tcttggaaac
    26641 aagtcttagc gatgtttcga ttggtgagat ttgtaacatt caggctggaa ttgaaagtaa
    26701 tgaaattgtt gcaagagctc aggttgtagg atttcatgat gaaaaaacaa tattaagctt
    26761 gattggaaat tctcgtggac tttcacggca aacgttgatt aagcccactg cccagtttct
    26821 tcatacgcaa gtcggccgtg gattattggg agcagtagtc aatcctttag gggaggttac
    26881 tgataagttt gctgttacag ataacagtga aattctttat cgacctgtag ataatgctcc
    26941 tccgctatat agtgaaaggg ctgcaattga gaagcctttt ttaacaggta ttaaggttat
    27001 tgattcttta ctcacgtgtg gtgaaggaca gcgaatgggg atttttgcgt cagctggttg
    27061 tggcaaaact tttctcatga atatgctcat tgaacatagt ggtgctgata tatatgttat
    27121 tgggttaatt ggtgagcgag gtcgagaggt tactgaaacg gttgattatt tgaaaaactc
    27181 tgagaaaaaa agcaggtgtg ttttagtata tgcaacttcg gattactctt cggttgatcg
    27241 ttgtaatgct gcatatatag ccactgctat agccgaattt tttaggactg aaggacataa
    27301 agtagcgctt tttattgatt cattaacaag gtatgccaga gcattacgtg atgtggcctt
    27361 agccgctgga gaatcacctg ccagaagagg ctatccggtt tcggtttttg atagcttacc
    27421 cagacttctt gaaaggccag gaaagttaaa ggcaggtggc tctattactg cattttacac
    27481 tgttcttttg gaggatgatg attttgctga tccattagct gaagaggtaa gatccatttt
    27541 agatggacat atatatttga gcagaaatct agcccaaaaa ggacaatttc ctgcaattga
    27601 ttccttaaaa agtataagca gggtatttac acaggttgtt gatgaaaaac atcgtattat
    27661 ggccgctgca tttcgggagc tactttctga aatagaagag ctaaggacaa ttattgactt
    27721 tggtgaatac aaaccggggg agaatgcctc tcaggataaa atctacaaca aaatatctgt
    27781 tgttgagagt tttctgaagc aagattatcg actcggtttt acttatgagc agacaatgga
    27841 gcttattggt gaaacaatta gataaggttt taaaaatcaa agataaatat caacggtctg
    27901 ttaaattaat tgaagcacac atattaacac taaaaaaaaa actctttgta tcgagatgtg
    27961 gaggcattag ataaaaggat tatatacttc ctgcaactgg agaatgactt agaacctgta
    28021 ggcgctcaga gtgttagtca gcttttcaat acaagacgta agatcgctat agtaaaaaag
    28081 catattatcc aatatcagtc tgaacgaatt cttctaaagg gaagaattga agaaattcaa
    28141 aaagatattg atgaggcaaa tgcttctaaa agaaaactgc ttcataaaga gagtaaaata
    28201 tgtaaacgga ttggcttgat taaaagaaat aactttgcca aacagttgat acttgatgag
    28261 ttatcacaag aggatatgaa gtatggcatt agataatata aacctaaatt ttagtagtga
    28321 caaacagata gaaaaatgtg agaaactatc ttcaatagat aatattgata gtctcgtttt
    28381 gaagaagaag aggaaggttg aaattcctga gtactcttta attgcatcta actattttac
    28441 tattgataag cactttgaac ataagcatga taaaggagaa atttatagtg gcattaaaaa
    28501 tgcgttcgaa cttagaaacg aacgagcgac atattctgat attccagaat caatggccat
    28561 taaagaaaat attttgatac cagatcaaga tatcaaagca agggaaaaaa taaatatcgg
    28621 cgatatgagg gggatctttt catataataa gagtggaaat gcagacaaga acttcgaaag
    28681 aagtcatact tcttctgtaa accctgataa tctgctagaa tctgataata gaaatggtca
    28741 aattggttta aaaaatcata gcttgtctat tgataagaat attgctgaca tcatttcttt
    28801 actaaatgga agtgttgcta aatcatttga gctgcctgta atgaataaaa atactgcaga
    28861 cataacccca tccatgtcat tgcaagaaaa atcaatagtt gaaaatgata aaaatgtttt
    28921 tcaaaaaaat agtgaaatga cttaccactt taaacagtgg ggggctggac attctgttag
    28981 tatttcagtg gagtctggtt cttttgttct aaaaccgtca gatcaatttg taggaaataa
    29041 acttgactta attttgaaac aagatgctga gggtaattac agatttgata gcagtcaaca
    29101 taataagggg aataaaaata atagtacagg atataatgaa cagagtgaag aagaatgcta
    29161 agaattaaac attttgacgc taacgaaaaa ctacagattt tatatgcaaa gcaactctgc
    29221 gagcgttttt cgattcagac attcaaaaat aaatttacag gcagtgaaag tttagtcact
    29281 cttacttccg tgtgtgggga ttgggtaatt cgtattgata cattatcttt tttgaaaaaa
    29341 aaatacgagg tattttcagg attttctaca caagaatctt tactgcattt atcaaaatgt
    29401 gtctttatag agtcgtcatc tgtattttcg attccagaac tgtctgataa gattactttc
    29461 cggatcacga atgaaatcca gtatgcaact actgggagtc atttatgctg tttttcctct
    29521 tctttaggta ttatttattt tgacaagatg ccggtattac gtaatcaagt ttctcttgac
    29581 tcattgcatc atcttttaga gttttgctta ggttcatcta atgtaaggct ggctacttta
    29641 aaaagaattc gcactggtga tataatcata gttcagaaac tttataattt attattgtgt
    29701 aatcaagtta ttattgggga ttatattgtg aatgataata atgaggcaaa aattaatctg
    29761 tcagaaagta atggtgagtc agaacacaca gaagtttctt tggcattatt caattatgat
    29821 gatatcaatg taaaagtgga ctttattctt ttagaaaaaa atatgacaat caatgaacta
    29881 aaaatgtatg tagaaaacga attattcaag tttcccgatg acatagttaa acatgtaaat
    29941 attaaagtaa atggttcttt ggttgggcat ggggaacttg tttctattga ggatggttat
    30001 ggtatcgaga ttagttcttg gatggtaaag gagtaatctc atgctgagtg acatgtccct
    30061 catcgctaca ctttcttttt ttacgctatt accattttta gttgctgctg gtacctgtta
    30121 tataaagttt tctattgttt ttgtaatggt acgaaatgca cttggtttgc agcaggttcc
    30181 atcaaatatg actctcaatg gtattgcatt gattatggct ttgttcgtga tgaaacccat
    30241 tattgaagct ggttatgaaa attatctaaa tggccctcaa aaatttgata caatatctga
    30301 tattgtccgt ttttctgatt ctggcttgat ggaatataag caatacttga aaaagcatac
    30361 agatcttgag ttagcacgtt ttttccaaag gtctgaagaa gaaaatgctg atttaaagag
    30421 tgcagagaat aatgattatt cattgttttc cctcttgcct gcatatgcgc taagtgaaat
    30481 caaagatgcg ttcaaaatag gtttttattt atatttgcca tttgttgttg ttgatcttgt
    30541 tatttcaagc attttacttg cacttggtat gatgatgatg agtccaatca caatatcagt
    30601 accaataaaa cttgtattat ttgttgcgct tgatgggtgg ggtatcctgt ctaaggcctt
    30661 gattgaacaa tatatcaata ttcctgctta gatgatggag gtaatgtgtc tgatatagtt
    30721 tatatgggta ataaggctct ttatttaatc cttatctttt ccttatggcc agtaggtata
    30781 gctacggtta ttggattatc tattggtttg ttacagacag tgactcaact ccaagagcag
    30841 acacttcctt ttggtataaa gcttataggt gtctcaatat ctttgctact tctttctgga
    30901 tggtatggtg aggttttatt gtctttttgt catgaaataa tgtttttaat taagagtggg
    30961 gtttgatgga catttcaagc tggttcgaaa gtattcatgt gtttttaata ctcctgaacg
    31021 gcgttttttt tagattggct ccattatttt tctttcttcc atttttaaat aacggtataa
    31081 tttctccatc tattagaata cctgtgattt ttcttgttgc atcaggatta attacttctg
    31141 gtaaggtaga cataggttct tctgtttttg aacatgttta tttccttatg ttcaaggaaa
    31201 taattgttgg cctccttctc tctttttgct tgtctcttcc cttttggata tttcatgctg
    31261 ttggtagcat tattgacaac cagcgtgggg caacgcttag tagttcaatt gatcctgcca
    31321 atggtgttga tacgtctgag ttggcaaaat ttttcaatct tttttctgca gttgtatttc
    31381 tatacagtgg tggtatggtc tttattttag aatccataca attgtcttat aatatatgcc
    31441 cgttattttc tcaatgttct ttccgtgtct caaatatctt aacatttctg actttattgg
    31501 caagtcaggc tgttatttta gccagtcctg ttatgatagt attgttacta tcagaagtat
    31561 tacttggtgt attatcgaga tttgctccgc agatgaatgc tttttccgta tcattaacta
    31621 ttaaaagttt acttgcaata tttattattt tcatctgttc ttctactatt tacttttcta
    31681 aagttcaatt tttcctcggt gaacataaat ttttcacaaa tctatttgtt agataaaata
    31741 ttatggcaaa taaaacagaa aagccgacac ctaaaaaact aaaggatgcc gcaaaaaaag
    31801 gacagtcatt taaatttaag gatttaacga ctgttgttat tattctggta gggacattta
    31861 ctataatatc attcttttcc ttaagtgatg taatgctctt atacagatat gtaataatta
    31921 atgacttcga aattaatgag ggtaaatact tttttgcagt ggttattgtc ttttttaaga
    31981 taattggctt cccacttttt ttctgtgttc tttcggctgt gttgccaaca ttggttcaaa
    32041 caaagtttgt tcttgcgact aaagctatca agattgattt ttcagtatta aaccctgtta
    32101 aagggttaaa aaaaatattt agtataaaga caataaaaga atttttcaaa agcattctgc
    32161 ttcttattat tctagcatta acaacctatt tcttttggat taatgaccga aaaataattt
    32221 tttctcaggt gttttctagt gttgatggct tatatcttat ttgggggagg ctgtttaagg
    32281 atataatatt atttttcttg gcattttcta ttcttgttat tattcttgac tttgtgattg
    32341 agttcatttt atacatgaaa gatatgatga tggataaaca ggagataaaa agagaatata
    32401 tagagcaaga gggacacttt gagacaaagt cgagaaggcg tgagttgcat atcgagattc
    32461 tttcagagca gactaaatct gatatacgta attcaaaatt agtggtaatg aacccgactc
    32521 atattgcaat tggtatttat tttaatccag aaatagcgcc tgcacctttt atttctctca
    32581 ttgaaactaa ccagtgtgcc ttggctgtca gaaaatatgc aaatgaagtt ggtataccga
    32641 ctgtgcgtga tgtgaaatta gctagaaaac tatataaaac acatacaaaa tatagttttg
    32701 ttgattttga acacttggat gaagtcctac gtcttattgt ttggcttgag caggttgaaa
    32761 acactcatta ataagtaaag gagatgtgtt atatgggagt taatttctgt aataaaatag
    32821 gtattgatca gtctgaattt gaaatagaat cttccatcat taactccatt gctaatgagg
    32881 tattgaaccc aatatctttc cttagcaata aggatataat aaatgtttta ctcagaaaga
    32941 tttcatctga atgtgacctg gttagaaaag acatttatcg ttgtgctctg gagttggtcg
    33001 ttgaaaaaac acctgatgat ttataatcat tcataataac tcttttgctg atataaatat
    33061 ttttgttgaa gatatttatg tagtcacagg atcaattgta gaaaattgtt atgtttttgt
    33121 taggaactgc actaaataat atatgataag acagcaaaaa cgactgacca taatccttct
    33181 tttattagga gttgataaga gagactattc atcctgtaat gttaaaactc tgttatatag
    33241 tattcgcgat tatgcaaagt ctgtaaatga tcatgagata ctaaccgaga gtaatcgttt
    33301 attatctcat tgcatttcag atagtaacgg agctttcttt aaaagtagta aatacgttcc
    33361 ccttaagtac ttacgtaaaa ggagaatagc acgaaagata ccaaatgatt agcactttgc
    33421 tagtaagctt gctggtaaac tatattgacc gttgggtttg ttttgagact gtaatttaaa
    33481 ttgtgtaatt gcctgttttt gatatgttta ctccaacaac ggagacaggt aaattatgga
    33541 cgaaaagaag cttaaagccc ttgcggctga actggctaaa ggcctcaaaa ctgaggccga
    33601 cctcaatcag ttttcccgta tgctgacgaa atatcccggc tcttaaaacg atagtggctc
    33661 aggctttcat ttttcagcgt tccccagaag atttccatcg gagcgttgtc gtaacagtta
    33721 cctttacgcg acattgatgt tttcagacca gactgctcct gtatgacccg gtaatcgtat
    33781 gcgcagtact gtgaacctcg agcggagtgg tggattagcc cggcaggtgg gtgctggctc
    33841 ctgagcgcca taaacagggc tttacccgtc aactctttcg tcatgcgctc tcccatggcg
    33901 taaccgacaa tttcgcacgt ataaacatct ttgacgccag cgagatacag ccatccttcc
    33961 cgggtggcaa catacgttat atctgcgacc cagacctgat taggtgctgt gggcgtaaag
    34021 ttctgattca gcagattcgg cgtaaccggc agattgtggt cagagcttgt ggtcgccctg
    34081 aacttgcgtt tctgcttaca gtgcagcctc agctccttac gaagacgtgc cagtctgtca
    34141 c
//
LOCUS       DAETQL010000042        33535 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.042, whole genome shotgun sequence.
ACCESSION   DAETQL010000042 DAETQL010000000
VERSION     DAETQL010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.042"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="K0W65_002146"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="K0W65_002146"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HBI3095407.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="K0W65_002147"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="K0W65_002147"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBI3095408.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="K0W65_002148"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="K0W65_002148"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBI3095409.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="K0W65_002149"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="K0W65_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBI3095410.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="K0W65_002150"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="K0W65_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBI3095411.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="K0W65_002151"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="K0W65_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBI3095412.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="K0W65_002152"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="K0W65_002152"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBI3095413.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="K0W65_002153"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="K0W65_002153"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBI3095414.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="K0W65_002154"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="K0W65_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBI3095415.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="K0W65_002155"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="K0W65_002155"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBI3095416.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="K0W65_002156"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="K0W65_002156"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBI3095417.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="K0W65_002157"
     CDS             8212..9075
                     /locus_tag="K0W65_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBI3095418.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="K0W65_002158"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="K0W65_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HBI3095419.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="K0W65_002159"
     CDS             10411..11028
                     /locus_tag="K0W65_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBI3095420.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="K0W65_002160"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="K0W65_002160"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HBI3095421.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="K0W65_002161"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="K0W65_002161"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBI3095422.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="K0W65_002162"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="K0W65_002162"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBI3095423.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="K0W65_002163"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="K0W65_002163"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBI3095424.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMGVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="K0W65_002164"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="K0W65_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBI3095425.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="K0W65_002165"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="K0W65_002165"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBI3095426.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="K0W65_002166"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="K0W65_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBI3095427.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="K0W65_002167"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="K0W65_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBI3095428.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="K0W65_002168"
     CDS             21859..23568
                     /locus_tag="K0W65_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBI3095429.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="K0W65_002169"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="K0W65_002169"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBI3095430.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="K0W65_002170"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="K0W65_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="K0W65_002171"
     tRNA            27625..27719
                     /locus_tag="K0W65_002171"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="K0W65_002172"
     CDS             27991..29202
                     /locus_tag="K0W65_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI3095431.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29614..30657
                     /locus_tag="K0W65_002173"
     CDS             29614..30657
                     /locus_tag="K0W65_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBI3095432.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31172..31600
                     /locus_tag="K0W65_002174"
                     /pseudo
     CDS             31172..31600
                     /locus_tag="K0W65_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase"
     gene            complement(31630..32781)
                     /locus_tag="K0W65_002175"
                     /pseudo
     CDS             complement(31630..32781)
                     /locus_tag="K0W65_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            32846..33494
                     /locus_tag="K0W65_002176"
                     /pseudo
     CDS             32846..33494
                     /locus_tag="K0W65_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8402 a         8815 c         8264 g         8054 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatgggtgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa
    29401 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
    29461 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
    29521 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
    29581 actatatcac cacatgtttt acaggctcag aaaatgaacg atagattatg ctttgaagtg
    29641 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
    29701 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
    29761 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
    29821 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
    29881 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
    29941 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
    30001 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
    30061 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
    30121 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
    30181 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
    30241 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
    30301 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
    30361 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
    30421 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
    30481 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
    30541 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
    30601 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
    30661 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
    30721 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
    30781 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
    30841 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
    30901 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
    30961 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
    31021 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
    31081 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
    31141 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
    31201 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
    31261 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
    31321 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
    31381 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
    31441 tgttacaccg atagtgacgc cagccttgct aagccgtgag ggttttttat atccgacgcc
    31501 agcgacctcc attcccagag acctgcttga acctttgcca aaaggagcat tttcgattgg
    31561 tgagattggg cagtatgaca aatacaagac gacccatact gatcctaccc acgtaatgtg
    31621 gacacggccc taagcgagat tatggttttc aaattgttcc gggctgagac cgccacaggc
    31681 actgtgacga cgccagcgat tgtaatcaca ctcgatataa ttaaacaccg ttgcccgcat
    31741 tatttcccgg ctgctaaagc gttccccgtg gatacattcc accttcagcg aatgaaagaa
    31801 gctttccaca caggcattgt cataacagta gcctttcgca ctcatactgc cacgcaggtt
    31861 gtgtcgcttc agcattgcct gataatcccc tgaacagtat tgaccaccac ggtccgtatg
    31921 aacaatgacg ctttccgggc gtcttctccg ccacaacgcc atttgcagtg catcacaggc
    31981 cagttgtgct gtcattcgcg gtgacatcga ccagccaatg acggcgcgtg accacaggtc
    32041 gatgactact gcgagataca accagccctc atcggtacgc aagtacgtga tgtcacccgc
    32101 ccacttctgg tttcgggcca ctggcgctga agtcctgctc cagcagattc tccaatacgg
    32161 gcaggccatg tgcacggtag ctgaccgggc tgaacttccg gctggctttc gcccgcagcc
    32221 cctgacgacg caggctggcg gcaatggttt taatattgaa ctccggcagt tcgtcagcaa
    32281 ggcggggagc accgtatcgc tgctttgcct caaagaatgc cttatggaca gcggcatcgc
    32341 aggtgagccg aaactgttgg cgcaggctca tctggtgacg acgcctgagc cagacatacc
    32401 agccgctgcg ggcaacccga agtacacgac acatcgcttt gatgctgaac tctgcccgat
    32461 gattttcgat aaagacatac ttcatttcag gcgcttcgcg aagtatgtcg cggccttttg
    32521 gaggatggac agttcctcag cctgctccgc cagttgtcgt ttaaggcgga cattttcagc
    32581 ggccagttcg ctttcgcgct ctgacgaact catttgttgc tgctgtttac tgcgccaggc
    32641 ataaagctga gattcataca ggctgagttc acgggctgcg gcggccacac cgatgcgttc
    32701 agcgagtttc agggcttcgt tacgaaattc aggcgtatgt tgtttacggg gcttcttgct
    32761 gattgatact ggttttgtca tgagtcacct ctggttgaga gtttactcac ttagtcctgt
    32821 gtccactatt ggtgggtaag atcaccctcc attaaaacta aaacgggcgc tttctgatat
    32881 atcatctgta agcgcagtaa taccattagt agcacctcgc gcattgatta ttgaatatta
    32941 taactggaat gttatatttc ttgttatgat gctgttcagt attataataa tgttattatc
    33001 accaaagata actcatgcaa atgagaaagt aatatgtcaa aacagtgcat cctttgtgac
    33061 caaagagttt atctgcggca ctatacttgt ctcattaata ctcagttctc tattttgcta
    33121 catatcaata tcaccatcaa ttttcatgca tacttatggc ctgagtactt ttagttactc
    33181 aattattttc tccttctcag ttttattttt cataatcgga aatcaattat caaagattga
    33241 aaaattatcc aacctatggt ttttattacc caatagccaa ttttatcgca ttacaaataa
    33301 atggttcaaa aacaggattt gccgcatcta ttacaggttt tactcaaaca ataagcgctg
    33361 gtacaatcag tttcctgtca gtaaccgtaa gcgacaacgg tgttggtccc ggaactactt
    33421 taggtcttgg ttcactaata acttcattac tgtcaatagc cgtaatcgcc attgcaggaa
    33481 ataataaaaa gtaagtgtac tctgactgcg tatgtatcta cttgaaccgc cccgg
//
LOCUS       DAETQL010000043        31186 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.043, whole genome shotgun sequence.
ACCESSION   DAETQL010000043 DAETQL010000000
VERSION     DAETQL010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.043"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            389..2047
                     /locus_tag="K0W65_002177"
     CDS             389..2047
                     /locus_tag="K0W65_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBI3095433.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2044..3000)
                     /locus_tag="K0W65_002178"
     CDS             complement(2044..3000)
                     /locus_tag="K0W65_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HBI3095434.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3151..4266
                     /gene="macA"
                     /locus_tag="K0W65_002179"
     CDS             3151..4266
                     /gene="macA"
                     /locus_tag="K0W65_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBI3095435.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4263..6209
                     /gene="macB"
                     /locus_tag="K0W65_002180"
     CDS             4263..6209
                     /gene="macB"
                     /locus_tag="K0W65_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBI3095436.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="K0W65_002181"
     CDS             complement(6282..6506)
                     /gene="cspD"
                     /locus_tag="K0W65_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBI3095437.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6829..7149
                     /gene="clpS"
                     /locus_tag="K0W65_002182"
     CDS             6829..7149
                     /gene="clpS"
                     /locus_tag="K0W65_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBI3095438.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7180..9456
                     /gene="clpA"
                     /locus_tag="K0W65_002183"
     CDS             7180..9456
                     /gene="clpA"
                     /locus_tag="K0W65_002183"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBI3095439.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9859..9946)
                     /locus_tag="K0W65_002184"
     tRNA            complement(9859..9946)
                     /locus_tag="K0W65_002184"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9910..9912),aa:Ser,seq:gga)
     gene            complement(10200..10418)
                     /gene="infA"
                     /locus_tag="K0W65_002185"
     CDS             complement(10200..10418)
                     /gene="infA"
                     /locus_tag="K0W65_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBI3095440.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10703..11407)
                     /gene="aat"
                     /locus_tag="K0W65_002186"
     CDS             complement(10703..11407)
                     /gene="aat"
                     /locus_tag="K0W65_002186"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBI3095441.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPDDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="K0W65_002187"
     CDS             complement(11449..13170)
                     /gene="cydC"
                     /locus_tag="K0W65_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBI3095442.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="K0W65_002188"
     CDS             complement(13171..14937)
                     /gene="cydD"
                     /locus_tag="K0W65_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBI3095443.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="K0W65_002189"
     CDS             complement(15060..16025)
                     /gene="trxB"
                     /locus_tag="K0W65_002189"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBI3095444.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16569..17063
                     /gene="lrp"
                     /locus_tag="K0W65_002190"
     CDS             16569..17063
                     /gene="lrp"
                     /locus_tag="K0W65_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBI3095445.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17198..21226
                     /gene="ftsK"
                     /locus_tag="K0W65_002191"
     CDS             17198..21226
                     /gene="ftsK"
                     /locus_tag="K0W65_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBI3095446.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21381..21992
                     /gene="lolA"
                     /locus_tag="K0W65_002192"
     CDS             21381..21992
                     /gene="lolA"
                     /locus_tag="K0W65_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBI3095447.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22003..23346
                     /gene="rarA"
                     /locus_tag="K0W65_002193"
                     /gene_synonym="mgsA"
     CDS             22003..23346
                     /gene="rarA"
                     /locus_tag="K0W65_002193"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HBI3095448.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23437..24729
                     /gene="serS"
                     /locus_tag="K0W65_002194"
     CDS             23437..24729
                     /gene="serS"
                     /locus_tag="K0W65_002194"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBI3095449.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24969..27412
                     /gene="dmsA"
                     /locus_tag="K0W65_002195"
                     /pseudo
     CDS             24969..27412
                     /gene="dmsA"
                     /locus_tag="K0W65_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27423..28040
                     /gene="dmsB"
                     /locus_tag="K0W65_002196"
     CDS             27423..28040
                     /gene="dmsB"
                     /locus_tag="K0W65_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBI3095450.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28042..28833
                     /locus_tag="K0W65_002197"
     CDS             28042..28833
                     /locus_tag="K0W65_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBI3095451.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28869..29495)
                     /locus_tag="K0W65_002198"
     CDS             complement(28869..29495)
                     /locus_tag="K0W65_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBI3095452.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29810..30958
                     /locus_tag="K0W65_002199"
     CDS             29810..30958
                     /locus_tag="K0W65_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3095453.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7689 a         7644 c         8374 g         7479 t
ORIGIN      
        1 tggtcgtttt atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa
       61 aaacgaaggc gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag
      121 cgggttgccc catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat
      181 cctttaggcg taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg
      241 ggcttatcag caagagggag tataacgcga ttattcgctc atttttcaga caattgccat
      301 gcttaaatgt gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa
      361 ggataagtca catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt
      421 tcgcggtatc aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa
      481 cgcgtggggt aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga
      541 tctctaccat tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga
      601 acatcatctg catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg
      661 ccgttatcgg ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt
      721 ttatcgtctg gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct
      781 cgataaagac ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt
      841 gcgtttaatg ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg
      901 cacggtgcca aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg
      961 tgagttgtcc tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc
     1021 aatggtgcag ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg
     1081 tttaatgcgg cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa
     1141 ccggatgatt gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc
     1201 tactttattg caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct
     1261 aattgaagat ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt
     1321 gaatcttttg cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac
     1381 tccagtggaa catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct
     1441 ggggccaagt ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa
     1501 tcgtccgtca tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg
     1561 ggttatcaat gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt
     1621 cattgagttt gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat
     1681 tgaatggcat gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg
     1741 cagcctgttg aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct
     1801 ggatatggaa cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca
     1861 aatcccggaa aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc
     1921 ttccaaaccc gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc
     1981 cgtaccaacg ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc
     2041 ggattaaccg cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg
     2101 ccgacggtat tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg
     2161 tgctggaagg cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc
     2221 gttgtaatca gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg
     2281 cgtttcattg ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc
     2341 cgcttccatc acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat
     2401 ttcctgatgt ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt
     2461 cccctgatat tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg
     2521 gaacaggatt gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt
     2581 gaactgctca ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata
     2641 taagctgaat tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc
     2701 ctccagcaat tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg
     2761 cagtcgcggc tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat
     2821 ccactcaacg cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact
     2881 acagcgatgc cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga
     2941 caggctgctg agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat
     3001 gaaatcaatg gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat
     3061 agtgtgatta ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc
     3121 ggattggcat atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt
     3181 tacgttattg cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac
     3241 gcacccgtgc cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg
     3301 ctggcgaccg gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt
     3361 cagttgaaaa cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg
     3421 gtcattgatc ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag
     3481 ctacgcgcgc agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc
     3541 cgtcagcaac gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg
     3601 acggagatgg ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat
     3661 caggcctctc tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg
     3721 gctggggaag tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa
     3781 gcaccgaaca ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct
     3841 gaagcggatg taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat
     3901 ccactgacgc gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac
     3961 gacgctattt tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg
     4021 gatatgaccg cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct
     4081 ctgtcggcgt taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat
     4141 ggtgaaacac gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt
     4201 gtcaaagggc tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca
     4261 caatgacgcc tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc
     4321 aggttgaggt gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg
     4381 ttggcgcttc gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg
     4441 ccaccagcgg cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc
     4501 tggcgcaact gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc
     4561 atttaaccgt cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac
     4621 agcgactgct tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt
     4681 attatccggc acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga
     4741 tgaacggcgg tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg
     4801 gcgaagaggt gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg
     4861 tcacccacga tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg
     4921 aaattgtgcg caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg
     4981 ttgtcaatac agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga
     5041 tggcatggcg ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta
     5101 ttatcggtat tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg
     5161 tgctggcgga tattcgttct attggtacga atactattga tgtctatccc gggaaagatt
     5221 ttggcgatga cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc
     5281 aaaaacaacc gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt
     5341 ataacaatgt tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct
     5401 atggcatgac cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc
     5461 aggtcgtggt tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg
     5521 ttggcgaggt gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa
     5581 aacagtcgat gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt
     5641 ccgggcgagt tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat
     5701 ttgacagcgc cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga
     5761 aggatttctt tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta
     5821 ctttacaact gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg
     5881 taatgaatat tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg
     5941 ctgtaggtgc gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt
     6001 gcctggtcgg tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc
     6061 ttttcttacc cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct
     6121 gctcgacggc caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg
     6181 atccagtaga tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc
     6241 tggcattttg cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct
     6301 acttcgacgg gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac
     6361 tgaacggatt gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga
     6421 gcgaaaatat cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg
     6481 aaccacttaa cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa
     6541 gatggaataa accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac
     6601 aatttagaga attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa
     6661 tcaaaaattt gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa
     6721 cgatagatga tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca
     6781 gcggcagggc gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg
     6841 aacgactggc tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg
     6901 ccatctatgt ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt
     6961 gacgtgttac aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct
     7021 gttcactacc aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa
     7081 gtggcgatgg tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa
     7141 aaagcctgaa tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa
     7201 ctcagtttaa atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc
     7261 gagcacttgt tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt
     7321 tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc
     7381 gttctgcctg ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta
     7441 ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac
     7501 gttctggtcg ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat
     7561 gaagtcagcc gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg
     7621 acacagtctt ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa
     7681 cgtatggaga atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca
     7741 ctaattggtc gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa
     7801 aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt
     7861 gcctggcgaa ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct
     7921 ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt
     7981 aaagcgttgc tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc
     8041 cacaccatta tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc
     8101 aaaccgttgc tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc
     8161 agcaacattt tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact
     8221 gaaccgtcga tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg
     8281 caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa
     8341 tacattaacg atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct
     8401 cgcgcacgtc tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag
     8461 tccgtggtgg cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat
     8521 accctgaaaa aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt
     8581 gaggcgctga ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg
     8641 gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag
     8701 ctttcgaaag cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc
     8761 cataccgtca gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt
     8821 ttgctgaccg atgcggtaat caagcatcca catgcggtgc tgctgctgga cgaaatcgag
     8881 aaagcgcatc cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc
     8941 gataacaacg gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc
     9001 ggggtacggg aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat
     9061 gcgatggaag agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt
     9121 atctggttcg atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc
     9181 gagttgcagg ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt
     9241 aactggctgg ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc
     9301 atccaggaca acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac
     9361 ggcggtcagg tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag
     9421 agtgcacaaa agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga
     9481 agtccgtaat ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc
     9541 ctatttttgg aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa
     9601 aaagggtgct gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag
     9661 tatttgagtt cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg
     9721 cgaacgcgtt cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc
     9781 ccaccatgtt cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta
     9841 cgagctcttc tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta
     9901 tacacacttt ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta
     9961 ccaaacctca tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga
    10021 agaattttaa tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac
    10081 atcgagacaa gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa
    10141 ggccggttaa accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat
    10201 cagcgactac ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc
    10261 actttgtcgc ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca
    10321 gtaaccacgt gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga
    10381 acggtacctt gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta
    10441 ccgtaatttg aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc
    10501 taaatcgcaa caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa
    10561 aagcgcaacg tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt
    10621 ccttatcggc agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg
    10681 gggagaaatg tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga
    10741 aattattcgg caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg
    10801 gaatttcgca ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct
    10861 taccgccatg accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt
    10921 ccatccggct gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac
    10981 cgccgacaag ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa
    11041 ggcgatggta ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg
    11101 cacagccttc aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt
    11161 tatgaaatcg cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg
    11221 gatcgggcga ccaccagagg atggggtcgt ctggagaaaa ccacggaaaa ataccacgct
    11281 ggtaagccat taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag
    11341 gctcacgtaa tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca
    11401 agcgcatgac cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg
    11461 cttgaactgg taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat
    11521 aatttgcccg ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg
    11581 ggtgaccatt aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct
    11641 ttcggttgtg gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg
    11701 taacagcgta cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc
    11761 ttcacctaac caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg
    11821 caagatctcc gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg
    11881 cagtgtggcg ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc
    11941 cgcttcattc aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc
    12001 ccatgcgcgg gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat
    12061 cgctatatgt tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg
    12121 ctccggataa gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt
    12181 ttgggtatca gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc
    12241 agaggcaatg acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa
    12301 cgcggctaac gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc
    12361 aacgccgcca gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat
    12421 tatcgcttgc gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat
    12481 ttctgtattc tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc
    12541 ttgcccttgc agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt
    12601 ttgcccggtg ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa
    12661 cataacgctg cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac
    12721 caccataatc accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc
    12781 gagcgtatca acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag
    12841 tccggcaggg gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac
    12901 gcggaaagtc gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat
    12961 tgcggcacca cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac
    13021 ccccgcaacc gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc
    13081 gagcagcgtc acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata
    13141 cagtgccaga tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa
    13201 tgtggcgaat gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg
    13261 gccatcctgc ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac
    13321 cattaacgtt gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact
    13381 gtgagcatca aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa
    13441 cgcacgggcc accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc
    13501 aacaggtgta tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc
    13561 cagcgctgct tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg
    13621 caattttgct gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga
    13681 ttctggtgat aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag
    13741 aaaaccagaa agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac
    13801 tgcacgttgg cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg
    13861 cgacgtgata aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc
    13921 ctcaccgcgc tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc
    13981 accaacagcc tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa
    14041 ctctggcgca aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg
    14101 accaaaatcc agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga
    14161 cagcgaggta aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat
    14221 tgtccgttgg cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg
    14281 accaaaaata cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg
    14341 agcaagagcg agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat
    14401 aaacaacgga attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg
    14461 gaagatcgcc accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata
    14521 gcgtgcatag taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc
    14581 cgcaggttta ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg
    14641 gcggatggca aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac
    14701 cacccatgcg cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc
    14761 acggggaata ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc
    14821 aatgatcaat atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga
    14881 gatgacgctt tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc
    14941 acgcttagca ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat
    15001 aaaggcgacc catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat
    15061 tattttgcgt cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca
    15121 gtaccggccg aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag
    15181 acgccaggaa tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag
    15241 ccgttttcca gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca
    15301 acaaacagac cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga
    15361 cgaacgccag tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc
    15421 agaatgatgt tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg
    15481 cggaaaccgt cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc
    15541 gcctcttcaa ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag
    15601 aaaccgtcgc aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc
    15661 aggccgagat agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg
    15721 ccgttatcgc cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca
    15781 aaaatgatct cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga
    15841 ccggtcagat cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg
    15901 ccgccttttt ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca
    15961 gcagcggtgt atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg
    16021 cccatgagat ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac
    16081 gtaaaaaaag agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca
    16141 ggtcaaattc tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat
    16201 taaatgatga ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg
    16261 ttttgctttg acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat
    16321 tcttatatgc gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg
    16381 tatggcgtgg acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc
    16441 accgtagacc caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt
    16501 agcgactctg aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga
    16561 caataataat ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta
    16621 acattcttaa tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg
    16681 tgggactttc cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta
    16741 ttcagggcta tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg
    16801 ttgagattac tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac
    16861 aaaaacttga agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga
    16921 aaacacgcgt gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc
    16981 tgcctggcgt caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc
    17041 gtctggttat taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca
    17101 ctcctgttaa tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc
    17161 atcgggcagg aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga
    17221 caaagaagtc acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat
    17281 ccttattgtc ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga
    17341 ccccagctgg tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg
    17401 tgcgtggttg gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt
    17461 cattattgtc ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga
    17521 ttattttgcc gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg
    17581 tctggcggca atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag
    17641 cttactaagc actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct
    17701 ctgcgtttgg gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga
    17761 aaaactcggc ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga
    17821 tgatacctgg gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca
    17881 cggcaaacag catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa
    17941 acggttggcg gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc
    18001 cggtaagcgg atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga
    18061 cccggacgac gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga
    18121 tccattatta aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac
    18181 ggcgacacaa agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc
    18241 tgttgatgtt ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac
    18301 gggagagccg gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca
    18361 gcctgcagtg caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta
    18421 tgcacctgca gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc
    18481 ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac
    18541 ataccagact gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc
    18601 gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc
    18661 cgcgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga
    18721 acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa
    18781 atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt
    18841 ttctccgctg gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt
    18901 tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg
    18961 gattggcccg cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc
    19021 gtcttacggt attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca
    19081 gcgcaatcag tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca
    19141 ggatgaactg gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca
    19201 acatgatgtg cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg
    19261 tcagtttgcg caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc
    19321 gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca
    19381 cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc
    19441 acagcagcag tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc
    19501 acaacagcag gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca
    19561 gcagcaatat cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca
    19621 acaaccggtt gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc
    19681 gcaggatacc ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa
    19741 accgacgacg ccactgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc
    19801 ggtagatacc tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt
    19861 ccgtattaaa gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact
    19921 taacctggct cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg
    19981 ttcactttcg acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg
    20041 tctggagtta ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc
    20101 caaattccgc gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga
    20161 gccggtggtt gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc
    20221 cggtaaatct gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga
    20281 agatgtgcgt ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat
    20341 tccgcacctg ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg
    20401 gtgtgttaac gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct
    20461 ggcgggttat aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc
    20521 gtactggaag ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata
    20581 cattgtcgtg ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga
    20641 agagctgata gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc
    20701 aactcagcgt ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg
    20761 tatcgccttt accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc
    20821 ggaatcactg ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt
    20881 acgtgtccat ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa
    20941 agcgcgtggt cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg
    21001 tgcgggtggt ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt
    21061 tgtcactgaa aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta
    21121 taaccgtgca gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg
    21181 gcacaacggt aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg
    21241 gataaggcgc ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc
    21301 tttcctcaag ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg
    21361 acgtaatttg aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct
    21421 tagtagcaag cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag
    21481 tcagcagctt ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc
    21541 aggaaggtca gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac
    21601 aacctgatga aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg
    21661 ttgagcaagc tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga
    21721 ttgcccgcaa ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact
    21781 ttgtcctgac gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac
    21841 gtgatggcac aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc
    21901 agctgaaatc ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc
    21961 aaggcgtcac ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc
    22021 gatttttcgg ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca
    22081 cagtatatcg gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa
    22141 gccgggcatt tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc
    22201 gctgaagtga ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct
    22261 ggcgtgaaag agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc
    22321 cgcactattc tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt
    22381 ctgccacata ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg
    22441 tttgagctta attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt
    22501 acagaggata ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt
    22561 ggtcaggata ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc
    22621 gacgcgcgcc gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat
    22681 agcggtaagc gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc
    22741 cgctttgata acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta
    22801 cgtggtagcg cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc
    22861 gatccgttat atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc
    22921 gatccacggg cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg
    22981 gcggaaggtg aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc
    23041 aacgctgtct acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat
    23101 gacgtgccgg ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg
    23161 caggaatatc gttatgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc
    23221 ccgccggaaa tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag
    23281 attggtgaaa agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac
    23341 cgttaatgtt atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc
    23401 acatctccca tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt
    23461 aatgagccag acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat
    23521 aagctgggcg ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa
    23581 gcggagcgta actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc
    23641 gagcctttac gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag
    23701 ctggatgctt tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca
    23761 gatgaagtgc cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc
    23821 ccgcgtgagt ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc
    23881 ctcgactttg cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag
    23941 attgctcgca tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat
    24001 ggctacagtg agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt
    24061 cagctgccga aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac
    24121 accagtaact atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt
    24181 gaaatcatcg atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt
    24241 tctgaagccg gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac
    24301 aaagttgaaa tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg
    24361 actggtcatg cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt
    24421 tgcactggcg acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg
    24481 gcacagaaca cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt
    24541 cgtatgcagg cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg
    24601 aacggttctg gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag
    24661 gctgatggtc gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat
    24721 attggctaat acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct
    24781 ccctttgata ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa
    24841 atctacctat ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg
    24901 ctatagaaat aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt
    24961 gagccattat gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg
    25021 gtttggtaaa aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac
    25081 cttttagtcg gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg
    25141 ttatctggag cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg
    25201 tggacggtga aatcaaatat gtcgaaacga caataccggc gatgacaatt acgacggcct
    25261 acactaggtt cgcgcctgcc tgcgtgggcg ttccatgcgt caccgtgtct acaatccgga
    25321 ccgcctgaaa tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat
    25381 tagctgggaa gaagcctacg acatcatcgc gaccaatatg cagcgcctga tcaaagagta
    25441 cggcaacgag tctatctatc tgaactatgg caccggtacg ctgggcggca ccatgacccg
    25501 ctcctggccg ccgggaaata ccctagtcgc gcgcctgatg aactgctgcg gcggttatct
    25561 taaccattac ggtgactact cttccgcgca aattgcggaa ggtttgaacc atacctacgg
    25621 cggctgggca gatggtaaca gcccgtcgga tatcgaaaac agtaaattgg tagtgctgtt
    25681 tggtaataac ccaggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca
    25741 ggcacgccag aaatctaatg ctcgcatgat catcatcgat ccgcgctata ccgacaccgg
    25801 tgccgggcgc gaagatgagt ggatccctat tcgtccagga acagatgccg cactggttaa
    25861 cggtctggcg tacgtcatga tcactaaaaa cctggtggat caggcattcc tcgataaata
    25921 ttgcgttggc tatgacgaga aaaccctgcc cgccagtgcg ccgaaaaatg gtcattataa
    25981 agcttatatt ctgggtgaag ggccagatgg cgtggctaaa acaccggaat gggcctcgca
    26041 aatcaccggt gttccggcag acaaaatcat caaactggct cgtgaaatcg gcagtaccaa
    26101 accggcgttt atcagccagg gatggggccc gcagcgtcac gctaacggtg aaatcgcaac
    26161 ccgtgctatc tcgatgctgg cgattctgac cggtaacgtt ggtattaatg gaggggacag
    26221 cggcgcgcgt gaaggttcat acagcttacc gtttgtccgt atgccgacct tggaaaaccc
    26281 gatccagacc agcatttcga tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat
    26341 gacggcgctg cgtgatggtg tgcgcgggaa agataagctg gatgtgccga tcaaaatgat
    26401 ctggaactat gccggtaact gcctgattaa ccagcattct gaaatcaacc gtacccatga
    26461 aatcctccag gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc
    26521 atcggcgaaa tatgctgaca tcctgctgcc tgactgcacc gcttccgaac agatggactt
    26581 tgcactggat gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa
    26641 accgcgcttt gaatgtaaga ccatctatga aatgaccagc gaactggcaa aacgtcttgg
    26701 cgttgagcaa cagtttactg aaggccgtac ccaggaagag tggatgcggc atctgtatgc
    26761 ccagtcgcgg gaagcgattc ctgaactgcc aacgtttgaa gagttccgca agcaggggat
    26821 ctttaaaaag cgcgacccac aagggcatca cgttgcttat aaagccttcc gtgaagatcc
    26881 gcaggcaaac ccactgacta cgccatcggg caaaattgag atttattcgc aggcgctggc
    26941 tgacattgcc gctacctggg aattgccaga aggcgatgtg atcgatccac tgccgatcta
    27001 cacgccgggc tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac
    27061 aggtttccac tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc
    27121 ggcttgccgt caggaaatgt ggatcaaccc gcttgatgcc caaaaacgcg gtatccacaa
    27181 cggcgataaa gtcaggatct ttaacgatcg tggtgaggtt catattgagg cgaaagtgac
    27241 gccacgaatg atgccgggcg tggtcgcact gggtgaaggc gcctggtatg acccggatgc
    27301 aaaacgtgtc gataagggcg gttgtatcaa cgtactgacc actcaacgtc cgtctcctct
    27361 cgctaagggg aatccgtcac atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac
    27421 cgatgacaac ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct
    27481 gcgagctggc ttgtaaagac tacaaagatt tgacgccaga agtcagtttc cgccgcattt
    27541 atgaatatgc tggcggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg
    27601 cctactatct gtcgatttcg tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc
    27661 cgagcggtgc gatgcataaa cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca
    27721 ttggctgccg ttactgtcat atggcttgcc cgtatggcgc accgcaatat aacgaaacga
    27781 aaggccatat gaccaaatgc gatggttgtt atgaccgtgt tgcggagggt aaaaagccga
    27841 tctgtgttga atcttgcccg ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta
    27901 aaaaacatgg cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga
    27961 atattgtgat caaacccaat gccaatagcc gcccgaccgg ggataccact ggctatctgg
    28021 caaacccgaa ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc
    28081 acggtcttcg ggcaatgtgt ggcgggtggt tttatcgttc tggctttggc gctgctcaaa
    28141 ggcgacctgc gagcagaagc ccagcagcgt gttatcgcct gcatgtttgg cttatgggta
    28201 ctgatgggca ttggctttat cgcctctatg ctccatcttg gttcaccgat gcgcgccttt
    28261 aactcgctca accgggtagg ggcttcagca ctcagtaacg aaatcgccag cggttcgatc
    28321 ttttttgctg taggcggcat cggctggttg ctggcaatgc tgaaaaagct gtcaccggca
    28381 ttgcgtacgc tatggctggt ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg
    28441 gtgcgtgtgt ataacagcat tgataccgtt ccgacctggt acagcatctg gacgccgatg
    28501 ggctgggcga tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggcgtg
    28561 atgtcagtga tgcagggcac agagctggca actattcata gttctgtgca gcaggccgca
    28621 gcgctggtgc cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc
    28681 ctgtgcttgt ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc
    28741 gtctcattca ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg
    28801 catatgaccg tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggccccg
    28861 gttctctttt atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca
    28921 ggaatatggt tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc
    28981 agctcgcaag ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga catgcgatcc
    29041 cacgccgaat gccgggtaat ttcattaaag gtccctgatg catcagtcac gacaaagaca
    29101 tcaaaccctt cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca
    29161 ccggcaataa ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta
    29221 tcccaggcgt taatattgcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc
    29281 agttctagaa ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag aatagttggt
    29341 aagttgaaat acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt
    29401 tcgatatccc gtacaaggga aagtaaacca gcctggtgat caacaagcaa aacggcagca
    29461 tcatttttat caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg
    29521 gtacgtctga aatcggtcag acgcgatgca ttgctctgaa agcatagacg ggaaatatga
    29581 gtttgctgtg accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca
    29641 ataaaaacaa tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata
    29701 taaatcgata ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg
    29761 gcggtggcat gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta
    29821 tacccggcct gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt
    29881 gttaaataca ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg
    29941 cgttgtcagc tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt
    30001 cattaaacgc attggcttta accgcagcta ttatctgtcc tccttcattt ttgctgctgg
    30061 ctgtgccggc cttggcctga tgattggttt ctggagctgg ttggcgtggc gttttgtcgc
    30121 gggcgtcggc tgtgccatga tttgggtggt tgttgagagc gcgctgatgt gcagtgggac
    30181 gtcacgtaac cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg tgggaacgtt
    30241 tttaggccag ttactggtca gcaaagtctc aaccgagctg atgagcgtat tgccgtgggt
    30301 tacaggtttg acattggcag ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca
    30361 ggctgaaaac catgattcga cgtcaattac ggcaatgcta aaactccgtc aggcgcggct
    30421 tggcgtgaat ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc
    30481 tctgtacctc aatcacaaag gggtgagcaa tgccagtatt ggtttctgga tggcggtact
    30541 ggtcagtgcg ggtatcctcg gacaatggcc gattggacgt ctggcggata agtttggtcg
    30601 actgctggtt ttgcgtgttc aggtctttgt cgtcattctc ggcagtattg cgatgcttag
    30661 ccaggcggcg atggccccag cgttattcat cctcggtgcc gctggcttta cgctatatcc
    30721 ggtggcgatg gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca
    30781 agccttactg ttgagctata ccgtgggaag tctgcttggc ccgtcattta gcgctatgct
    30841 aatgcagaat ttctccgata atttattgtt tatcatgatc gccagcgtat cgtttatcta
    30901 tttgctgatg ctgcttcgca acgccggtca tacgccgaaa cccgttgctc acgtgtaaat
    30961 gaattcaagc aaagtgtgaa cttactgttt cacactctgc ttttttgttt cttctatctg
    31021 acttgctttt ttccaaattt tattcgttta aaaataaaat gtgcagcagg ttataatttt
    31081 gcatttcgct atttccgcac ttcttatttg ccgcgcataa tccctcgttt tacagatgcc
    31141 cctttaattt tggcgaagga tttgtctatg gctgggaatg ttcagg
//
LOCUS       DAETQL010000044        30666 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.044, whole genome shotgun sequence.
ACCESSION   DAETQL010000044 DAETQL010000000
VERSION     DAETQL010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30666
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.044"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..588
                     /gene="tuf"
                     /locus_tag="K0W65_002200"
     CDS             <1..588
                     /gene="tuf"
                     /locus_tag="K0W65_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBI3095454.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            818..1201
                     /gene="secE"
                     /locus_tag="K0W65_002201"
     CDS             818..1201
                     /gene="secE"
                     /locus_tag="K0W65_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBI3095455.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1203..1748
                     /gene="nusG"
                     /locus_tag="K0W65_002202"
     CDS             1203..1748
                     /gene="nusG"
                     /locus_tag="K0W65_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBI3095456.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1907..2335
                     /gene="rplK"
                     /locus_tag="K0W65_002203"
     CDS             1907..2335
                     /gene="rplK"
                     /locus_tag="K0W65_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBI3095457.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2339..3043
                     /gene="rplA"
                     /locus_tag="K0W65_002204"
     CDS             2339..3043
                     /gene="rplA"
                     /locus_tag="K0W65_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBI3095458.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3456..3953
                     /gene="rplJ"
                     /locus_tag="K0W65_002205"
     CDS             3456..3953
                     /gene="rplJ"
                     /locus_tag="K0W65_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBI3095459.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4020..4385
                     /gene="rplL"
                     /locus_tag="K0W65_002206"
     CDS             4020..4385
                     /gene="rplL"
                     /locus_tag="K0W65_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBI3095460.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4705..8733
                     /gene="rpoB"
                     /locus_tag="K0W65_002207"
     CDS             4705..8733
                     /gene="rpoB"
                     /locus_tag="K0W65_002207"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBI3095461.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8810..13033
                     /gene="rpoC"
                     /locus_tag="K0W65_002208"
     CDS             8810..13033
                     /gene="rpoC"
                     /locus_tag="K0W65_002208"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBI3095462.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="K0W65_002209"
     CDS             complement(13275..14408)
                     /gene="thiH"
                     /locus_tag="K0W65_002209"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBI3095463.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="K0W65_002210"
     CDS             complement(14405..15175)
                     /gene="thiG"
                     /locus_tag="K0W65_002210"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBI3095464.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="K0W65_002211"
     CDS             complement(15177..15377)
                     /gene="thiS"
                     /locus_tag="K0W65_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBI3095465.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="K0W65_002212"
     CDS             complement(15361..16116)
                     /gene="thiF"
                     /locus_tag="K0W65_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBI3095466.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="K0W65_002213"
     CDS             complement(16109..16744)
                     /gene="thiE"
                     /locus_tag="K0W65_002213"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBI3095467.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="K0W65_002214"
     CDS             complement(16744..18639)
                     /gene="thiC"
                     /locus_tag="K0W65_002214"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBI3095468.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18720..18820)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="K0W65_002215"
     CDS             complement(18873..19349)
                     /gene="rsd"
                     /locus_tag="K0W65_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HBI3095469.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19444..20217
                     /gene="nudC"
                     /locus_tag="K0W65_002216"
     CDS             19444..20217
                     /gene="nudC"
                     /locus_tag="K0W65_002216"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBI3095470.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20257..21321
                     /gene="hemE"
                     /locus_tag="K0W65_002217"
     CDS             20257..21321
                     /gene="hemE"
                     /locus_tag="K0W65_002217"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBI3095471.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21478..22149
                     /gene="nfi"
                     /locus_tag="K0W65_002218"
     CDS             21478..22149
                     /gene="nfi"
                     /locus_tag="K0W65_002218"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBI3095472.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22192..22782
                     /locus_tag="K0W65_002219"
     CDS             22192..22782
                     /locus_tag="K0W65_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBI3095473.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22969..23241
                     /gene="hupA"
                     /locus_tag="K0W65_002220"
     CDS             22969..23241
                     /gene="hupA"
                     /locus_tag="K0W65_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBI3095474.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23254..23949
                     /locus_tag="K0W65_002221"
     CDS             23254..23949
                     /locus_tag="K0W65_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBI3095475.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="K0W65_002222"
     CDS             complement(23951..24376)
                     /gene="zraP"
                     /locus_tag="K0W65_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBI3095476.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24614..25990
                     /gene="zraS"
                     /locus_tag="K0W65_002223"
     CDS             24614..25990
                     /gene="zraS"
                     /locus_tag="K0W65_002223"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBI3095477.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25987..27312
                     /gene="zraR"
                     /locus_tag="K0W65_002224"
     CDS             25987..27312
                     /gene="zraR"
                     /locus_tag="K0W65_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBI3095478.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27309..28598)
                     /gene="purD"
                     /locus_tag="K0W65_002225"
     CDS             complement(27309..28598)
                     /gene="purD"
                     /locus_tag="K0W65_002225"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBI3095479.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28610..30199)
                     /gene="purH"
                     /locus_tag="K0W65_002226"
     CDS             complement(28610..30199)
                     /gene="purH"
                     /locus_tag="K0W65_002226"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBI3095480.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7191 a         7982 c         8440 g         7053 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggg cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggtcgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc tgagctaatt gccgataaca
      601 tttgacgcaa tgcgcactaa aagggcatca tttgatgccc tttttgcacg ctttcgtacc
      661 agaacctggc tcatcagtga ttttctttgt cataatcatt gctgagacag gctctgttga
      721 gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg tttgcctcgc gatcgcgggg
      781 tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg agtgcgaata ccgaagctca
      841 aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt gtggtggcat tgctcctggc
      901 ggcgattgtc ggcaactacc tttatcgcga cattatgctg ccgctgcgtg cgctggccgt
      961 agtaattctg attgctgcag cgggtggtgt cgcgctgtta acgacaaaag gtaaagctac
     1021 cattgctttt gcccgtgaag cgcgtaccga agtccgtaag gtcatttggc cgactcgcca
     1081 ggaaacattg cacaccacgc tgattgtggc tgcggttacc gcagtaatgt cactgatcct
     1141 gtggggactg gatggtattc tggttcgcct ggtatccttt atcactggcc tgaggttctg
     1201 agatgtctga agctcctaaa aagcgctggt acgtcgttca ggcgttttcc ggttttgaag
     1261 gccgcgtagc aacgtcgctg cgtgagcata tcaaattaca caacatggaa gatttgtttg
     1321 gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg tggcggtcag cgtcgcaaaa
     1381 gcgaacgtaa attcttccct ggctacgtcc tcgttcagat ggtgatgaac gacgcgagct
     1441 ggcacttggt gcgcagcgta ccgcgtgtga tgggcttcat cggcggtact tccgatcgtc
     1501 ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa ccgcctgcag caggttggtg
     1561 ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat ggtccgtgtt aatgatggtc
     1621 cgttcgctga cttcaacggt gttgttgaag aagtggatta cgagaaatct cgtctgaaag
     1681 tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct ggacttcagc caggttgaaa
     1741 aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt tgcacaaggc gtgagattgg
     1801 aatacaattt cgcgcctttt gtttttatgg gccttgcccg taaaacgatt ttttatatca
     1861 cggggagcct ctcagaggcg ttattaccca acttgaggaa tttataatgg ctaagaaagt
     1921 acaagcctat gtcaagctgc aggttgcagc tggtatggct aacccgagtc cgccagtagg
     1981 tccggctctg ggtcagcagg gcgtaaacat catggaattc tgcaaagcgt tcaacgcaaa
     2041 aactgattcc atcgaaaaag gtctgccgat tccggtagta atcaccgttt acgctgaccg
     2101 ttctttcact ttcgttacca agaccccgcc ggcagcagtt ctgctgaaaa aagcggcagg
     2161 tatcaagtct ggttccggta agccgaacaa agacaaagtg ggtaaaattt cccgcgctca
     2221 gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact ggtgccgaca ttgaagcgat
     2281 gactcgctcc atcgaaggta ctgcacgttc catgggcctg gtagtggagg actaagaaat
     2341 ggctaaactg accaagcgca tgcgtgttat ccgtgagaaa gttgatgcaa ccaaacagta
     2401 cgacatcaac gaagctatcg cactgctgaa agagctggcg actgctaaat tcgtagaaag
     2461 cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa tctgaccaga acgtacgtgg
     2521 tgcaactgta ctgccgcacg gtactggccg ttccgttcgc gtagccgtat ttacccaagg
     2581 tgcaaacgct gaagctgcta aagctgcagg cgcagaactg gtaggtatgg aagatctggc
     2641 tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt attgcttctc cggatgcaat
     2701 gcgcgttgtt ggccagctgg gccaggttct gggtccgcgt ggcctgatgc caaacccgaa
     2761 agtgggtact gtaacaccga acgttgctga agcggttaaa aacgctaaag ctggccaggt
     2821 tcgttaccgt aacgacaaaa acggcatcat ccacaccacc atcggtaaag tggactttga
     2881 cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt gcgctgaaaa aagcaaaacc
     2941 gactcaggcg aaaggcgtgt acatcaagaa agttagcatc tccaccacca tgggtgcagg
     3001 tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac taatgccttt acgtgggcgg
     3061 tgattttgtc tacaatctta cccccacgta taatgcttaa tgcagacgta tatccgagat
     3121 attcgggttg tggcaaggcg gcaactgagt gagtcgccaa gagcatagct aactatgtga
     3181 ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa tagcgacgga tagaaaagat
     3241 ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag accgcaggag tttcgcaaga
     3301 aacttaatcc cctgcgtaga cggtgacaga acgctaagat tattctttta tattctggct
     3361 tgtttctgct caccgtaatt aagacgctct ctccgtttgg aggagtgaag tgagttccag
     3421 agattttctc tggcaaacat ccaggagcaa agctaatggc tttaaatctt caagacaaac
     3481 aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc gctgtctgca gtagttgcgg
     3541 attcccgtgg cgtaactgta gataaaatga ctgaactgcg taaagcaggt cgcgaagctg
     3601 gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg tgctgttgaa ggtactccgt
     3661 tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat tgcatactct atggaacacc
     3721 cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc gaatgcaaaa tttgaggtca
     3781 aagccgctgc ctttgaaggt gagctgatcc cggcgtctca gatcgaccgc ctggcaactc
     3841 tgccgaccta cgaagaagca attgcacgcc tgatggcaac catgaaagaa gcttcggctg
     3901 gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa agaagctgct taatcgcagt
     3961 tatcttttta acgcattcgc ttacgtataa acttattctg atattcagga acaatttaaa
     4021 tgtctatcac taaagatcaa atcattgaag cagttgcagc tatgtctgta atggacgttg
     4081 tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc cgctgctgct gctgtagctg
     4141 tagctgctgg cccggttgaa gctgctgaag aaaaaactga attcgacgta attctgaaag
     4201 ctgctggcgc taacaaagtt gctgttatca aagcagtacg tggcgcaact ggcctgggtc
     4261 tgaaagaagc taaagacctg gtagaatctg caccggctgc tctgaaagaa ggcgtgagca
     4321 aagacgacgc agaagcactg aaaaaagctc tggaagaagc tggcgctgaa gttgaagtta
     4381 aataagccaa cccttccggt tgcagcctga gaaatcaggc tgatggctgg tgacttttta
     4441 gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt cacactgttt gactactgct
     4501 gtgcctttca atgcttgttt ctatcgacga cttaatatac tgcgacagga cgtccgttct
     4561 gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca ggcattgcgg aaagtgttcc
     4621 attttccggt caacaaaata gtgttgcaca aactgtccgc tcaatggaca gatgggtcga
     4681 cttgtcagcg agctgaggaa ccctatggtt tactcctata ccgagaaaaa acgtattcgt
     4741 aaggattttg gtaaacgtcc acaagttctg gatgtacctt atctcctttc tatccagctt
     4801 gactcgtttc agaaatttat cgagcaagat cctgaagggc agtatggtct ggaagctgct
     4861 ttccgttccg tattcccgat tcagagctac agcggtaatt ccgagctgca atacgtcagc
     4921 taccgccttg gcgaaccggt gtttgacgtc caggaatgtc aaatccgtgg cgtgacctat
     4981 tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg agcgcgaagc gccggaaggc
     5041 accgtaaaag acattaaaga acaagaagtc tacatgggcg aaattccgct catgacagac
     5101 aacggtacct ttgttatcaa cggtactgag cgtgttatcg tttcccagct gcaccgtagt
     5161 ccgggcgtct tctttgactc cgacaaaggt aaaacccact cttcgggtaa agtgctgtat
     5221 aacgcgcgca tcatccctta ccgtggttcc tggctggact tcgaattcga tccgaaggac
     5281 aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg cgaccatcat tctgcgcgcc
     5341 ctgaactaca ccacagagca gatcctcgac ctgttctttg aaaaagttat ctttgaaatc
     5401 cgtgataaca agctgcagat ggaactggtg ccggaacgcc tgcgtggtga aaccgcatct
     5461 tttgacatcg aagctaacgg taaagtgtac gtagaaaaag gccgccgtat cactgcgcgc
     5521 cacattcgcc agctggaaaa agacgacgtc aaactgatcg aagtcccggt tgagtacatc
     5581 gcaggtaaag tggttgctaa agactatatt gatgagtcta ccggcgagct gatctgcgca
     5641 gcgaacatgg agctgagcct ggatctgctg gctaagctga gccagtctgg tcacaagcgt
     5701 atcgaaacgc tgttcaccaa cgatctggat cacggcccat atatctctga aaccttacgt
     5761 gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa tctaccgcat gatgcgccct
     5821 ggcgagccgc cgactcgtga agcagctgaa agcctgttcg agaacctgtt cttctccgaa
     5881 gaccgttatg acttgtctgc ggttggtcgt atgaagttca accgttctct gctgcgcgaa
     5941 gaaatcgaag gttccggtat tctgagcaaa gacgacatca ttgatgttat gaaaaagctc
     6001 atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg accacctcgg caaccgtcgt
     6061 atccgttccg ttggcgaaat ggcggaaaac cagttccgcg ttggcctggt acgtgtagag
     6121 cgtgcggtga aagagcgtct gtctctgggc gatctggata ccctgatgcc tcaggatatg
     6181 atcaacgcca agccgatttc cgcagcagtg aaagagttct tcggttccag ccagctgtct
     6241 cagtttatgg accagaacaa cccgctgtct gagattacgc acaaacgtcg tatctccgca
     6301 ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg aagttcgaga cgtacacccg
     6361 actcactacg gtcgcgtatg tccaatcgaa acccctgaag gtccgaacat cggtctgatc
     6421 aactctctgt ccgtgtacgc acagactaac gaatacggct tccttgagac tccgtatcgt
     6481 aaagtgaccg acggtgttgt aactgacgaa attcactacc tgtctgctat cgaagaaggc
     6541 aactacgtta tcgcccaggc gaactccaac ctggatgaag aaggccactt cgtagaagac
     6601 ctggtaactt gccgtagcaa aggcgaatcc agcttgttca gccgcgacca ggttgactac
     6661 atggacgtat ccacccagca ggtggtatcc gtcggtgcgt ccctgatccc gttcctggaa
     6721 cacgatgacg ccaaccgtgc attgatgggt gcgaatatgc aacgtcaggc cgttccgact
     6781 ctgcgcgctg ataagccgct ggttggtact ggtatggaac gtgctgttgc cgttgactcc
     6841 ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt acgtggatgc ttcccgtatc
     6901 gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag caggtatcga catctacaac
     6961 ctgaccaaat acacccgttc taaccagaac acctgtatca accagatgcc gtgtgtgtct
     7021 ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg gtccgtccac cgacctcggt
     7081 gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc cgtggaatgg ttacaacttc
     7141 gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag accgtttcac caccatccac
     7201 attcaggaac tggcgtgtgt gtcccgtgac accaagctgg gtccggaaga gatcaccgct
     7261 gacatcccga acgtgggtga agctgcgctc tccaaactgg atgaatccgg tatcgtttac
     7321 attggtgcgg aagtgaccgg tggcgacatt ctggttggta aggtaacgcc gaaaggtgaa
     7381 actcagctga ccccagaaga aaaactgctg cgtgcgatct tcggtgagaa agcctctgac
     7441 gttaaagact cttctctgcg cgtaccaaac ggtgtatccg gtacggttat agacgttcag
     7501 gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc tggaaatcga agaaatgcag
     7561 ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga tcctcgaagc gggtctgttc
     7621 agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag ctgagaagct cgacaaactg
     7681 ccgcgcgatc gctggctgga gctgggcctg acagacgaag agaaacaaaa tcagctggaa
     7741 cagctggctg agcagtatga cgaactgaaa cacgagttcg agaagaaact cgaagcgaaa
     7801 cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg tgctgaagat tgttaaggta
     7861 tatctggcgg ttaaacgccg tatccagcct ggtgacaaga tggcaggtcg tcacggtaac
     7921 aagggtgtaa tttctaagat caacccgatc gaagatatgc cttacgatga aaacggtacg
     7981 ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc gtatgaacat cggtcagatc
     8041 ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg acaagatcaa cgccatgctg
     8101 aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc agcgtgcgta cgatctgggc
     8161 gctgacgttc gtcagaaagt tgacctgagt accttcagcg atgaagaagt tatgcgtctg
     8221 gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg tgttcgacgg tgcgaaagaa
     8281 gcagaaatta aagagctgct gaaacttggc gacctgccga cttccggtca gatccgcctg
     8341 tacgatggtc gcactggtga acagttcgag cgtccggtaa ccgttggtta catgtacatg
     8401 ctgaaactga accacctggt cgacgacaag atgcacgcgc gttccaccgg ttcttacagc
     8461 ctggttactc agcagccgct gggtggtaag gcacagttcg gtggtcagcg tttcggggag
     8521 atggaagtgt gggcgctgga agcatacggc gcagcataca ccctgcagga aatgctcact
     8581 gttaagtctg atgacgtgaa cggtcgtacc aagatgtata aaaacattgt ggacggcaac
     8641 catcagatgg agccgggcat gccagaatcc ttcaacgtat tgttgaaaga gattcgttcg
     8701 ctgggtatca acatcgaact ggaagacgag taattctcgc tcaaacaggt cactgctgtc
     8761 ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg agcaaatccg tgaaagattt
     8821 attaaagttt ctgaaagcgc agactaaaac cgaagagttt gatgcgatca aaattgctct
     8881 ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa gttaaaaagc cggaaaccat
     8941 caactaccgt acgttcaaac cagaacgtga cggccttttc tgcgcccgta tctttgggcc
     9001 ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc ctgaaacacc gtggcgtcat
     9061 ctgtgagaag tgcggcgttg aagtgaccca gactaaagta cgccgtgagc gtatgggcca
     9121 catcgaactg gcttccccga ctgcgcacat ctggttcctg aaatcgctgc cgtcccgtat
     9181 cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc gtactgtact ttgaatccta
     9241 tgtggttatc gaaggcggta tgaccaacct ggaacgtcag cagatcctga ctgaagagca
     9301 gtatctggac gcgctggaag agttcggtga cgaattcgac gcgaagatgg gtgcggaagc
     9361 aatccaggct ctgctgaaga gcatggatct ggagcaagag tgcgaacagc tgcgtgaaga
     9421 gctgaacgaa accaactccg aaaccaagcg taaaaagctg accaagcgta tcaaactgct
     9481 ggaagcgttc gttcagtctg gtaacaaacc agagtggatg atcctgaccg ttctgccggt
     9541 actgccgcca gatctgcgtc cgctggttcc gctggatggt ggtcgtttcg cgacttctga
     9601 cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac cgtctgaaac gtctgctgga
     9661 tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt atgctgcagg aagcggtaga
     9721 cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc ggttctaaca agcgtcctct
     9781 gaaatctttg gccgacatga tcaaaggtaa acagggtcgt ttccgtcaga acctgctcgg
     9841 taagcgtgtt gactactccg gtcgttctgt aatcaccgta ggtccatacc tgcgtctgca
     9901 tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc aaaccgttca tctacggcaa
     9961 gctggaactg cgtggtcttg ctaccaccat taaagctgcg aagaaaatgg ttgagcgcga
    10021 agaagctgtc gtttgggata tcctggacga agttatccgc gaacacccgg tactgctgaa
    10081 ccgtgcaccg actctgcacc gtctgggtat ccaggcattt gaaccggtac tgatcgaagg
    10141 taaagctatc cagctgcacc cgctggtttg tgcggcatat aacgccgact tcgatggtga
    10201 ccagatggct gttcacgtac cgctgacgct ggaagcccag ctggaagcgc gtgcgctgat
    10261 gatgtctacc aacaacatcc tgtccccggc gaacggcgaa ccaatcatcg ttccgtctca
    10321 ggacgttgta ctgggtctgt actacatgac ccgtgactgt gttaacgcca aaggcgaagg
    10381 catggtgctg actggcccga aagaagcaga acgtctgtat cgctctggtc tggcttctct
    10441 gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa gatgctaacg gtgaattagt
    10501 agcgaaaacc agcctgaaag acacgactgt tggccgtgcc attctgtgga tgattgtacc
    10561 gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt aaaaaagcaa tctccaaaat
    10621 gctgaacacc tgctaccgca ttctcggtct gaaaccgacc gttatttttg cggaccagat
    10681 catgtacacc ggcttcgcct atgcagcgcg ttctggtgca tctgttggca tcgatgacat
    10741 ggtcatcccg gagaagaaac acgaaatcat ctccgaggca gaagcagaag ttgctgaaat
    10801 tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa cgctacaaca aagttatcga
    10861 tatctgggct gcggcgaacg atcgtgtatc caaagcgatg atggataacc tgcaaactga
    10921 aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt tccttcaaca gcatctacat
    10981 gatggccgac tccggtgcgc gtggttctgc ggcacagatt cgtcagcttg ctggtatgcg
    11041 tggtctgatg gcaaagccgg atggctccat catcgaaacg ccaatcaccg cgaacttccg
    11101 tgaaggtctg aacgtactcc agtacttcat ctccacccac ggtgctcgta aaggtctggc
    11161 ggataccgca ctgaaaactg cgaactccgg ttacctgact cgtcgtctgg ttgacgtggc
    11221 gcaggacctg gtggttaccg aagacgattg tggtacccat gaaggtatca tgatgactcc
    11281 ggttatcgag gggggtgacg ttaaagagcc gctgcgcgat cgcgtactgg gtcgtgtaac
    11341 tgctgaagac gttctgaagc cgggtactgc tgatattctc gttccgcgca acacgctgct
    11401 gcacgaacag tggtgtgacc tgctggaaga gaactctgtc gacgcggtaa aagtacgttc
    11461 tgttgtatct tgtgacaccg actttggtgt atgtgcgcac tgctacggtc gtgacctggc
    11521 gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt atcgcggcac agtccatcgg
    11581 tgaaccgggt acacagctga ccatgcgtac gttccacatc ggtggtgcgg catctcgtgc
    11641 ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc atcaagctca gcaacgtgaa
    11701 gtcggttgtg aactccagcg gtaaactggt tatcacttcc cgtaacaccg aactgaaact
    11761 gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg ccttacggtg cggtactggc
    11821 gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt gcaaactggg acccgcacac
    11881 catgccggtt atcaccgaag taagcggttt tgtacgcttt actgacatga tcgacggcca
    11941 gaccattact cgtcagaccg acgaactgac cggtctgtct tcgctggtgg ttctggattc
    12001 cgcagaacgt accgcaggtg gtaaagatct gcgtccggca ctgaaaatcg ttgatgctca
    12061 gggtaacgac gttctgatcc caggtaccga tatgcctgcg cagtacttcc tgccgggtaa
    12121 agcgattgtt cagctggaag atggcgtaca gatcagctct ggtgacacct tggcgcgtat
    12181 tccgcaggaa tccggcggta ccaaggacat caccggtggt ctgccgcgcg ttgcggacct
    12241 gttcgaagct cgtcgtccga aagagccggc aatcctggct gaaatcagcg gtatcgtttc
    12301 cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc accccggtag acggtagcga
    12361 tccgtacgaa gagatgattc cgaaatggcg tcagctcaac gtgttcgaag gtgaacgtgt
    12421 agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg cacgacattc tgcgtctgcg
    12481 tggtgttcat gctgtgactc gttacatcgt taacgaagta caggacgtat accgtctgca
    12541 gggcgtaaag attaacgata aacacatcga agttatcgtt cgtcagatgc tgcgtaaagc
    12601 taccatcgtt aacgcgggca gctccgactt cctggaaggc gaacaggttg aatactctcg
    12661 cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa gtgggtgcaa cttactcccg
    12721 cgatctgctg ggtatcacca aagcgtctct ggcaaccgag tccttcatct ccgcggcatc
    12781 gttccaggag accactcgcg tgctgaccga agcagccgtt gcgggcaaac gcgacgaact
    12841 gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc ccggcaggta ccggttacgc
    12901 gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa gctccggctg caccgcaggt
    12961 gactgcagaa gacgcatctg ccagcctggc agaactgctg aacgcaggtc tgggcggttc
    13021 tgataacgag taatcgttaa tccgcaaata acgtaaaaac ccgcttcggc gggttttttt
    13081 atgggtgtta ttcagagggc aaattcttca cggaaaatat taagaaaggc agggcgaaac
    13141 gaggaagaag cttttcgccc tggacaacat tcctgctgac ggcactacca taaaaaaaca
    13201 caacgctttc aacaaccttg cgtttttcat cttccgtagc ggctcggaat aaatttacgt
    13261 tcttacattc cgtctcatag tctttgcgag gcgcgtccca gatagctgtc ccagtctttc
    13321 cataccggct gcaaaccctg agcggttaac acggcagcaa ccgcttccgg tctgcgatcg
    13381 tcgtgcggtg agaactgttc caactcgggg tgattatcgg catagccacc tggctgcgtt
    13441 ttcgagaagg cgctgacgtt attgatcgcc agcggaataa cgcgatcgcg aaaccacggt
    13501 gattcccgcg tggagagtga cagttcaatc tccggtgcaa gcagtcggaa ggcgcagatg
    13561 gtttgcacta actggcgttc atccataatc gacgcaggct caatgccgcc agtacacggg
    13621 cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt aatgctgttg cagccatagc
    13681 aaatgttcgg caaccatata gcagtcaacg cgccagttgt cggaaaggcc aattagcgcg
    13741 ccgaggccta tcttatcaat ccccgcacgc cccagccgat ccggcgtttc cagccgccag
    13801 aagaagtcct gttttttgcc tttcaaatga tggcgggtat aagtcgcctc gtgatatgtc
    13861 tcctgataaa ccatcacccc atccagacca agttgcttta actcggcgta ttccgtctcc
    13921 gccagcggtt gcacttccat ttgtagtgaa gagaactgtt cacgcagggc ggggagatga
    13981 cgacgaaagt aatccatccc cactttcgcc tgatgttcac cagtgactaa tagcagatgt
    14041 tcaaagccca tctcccgtat agcggcgctt tccctggcaa tatccgcttc atccagcgtt
    14101 ttgcgcttga tgcgattact catggaaaat ccgcagtacg tgcagtcgtt agcgcaaaga
    14161 ttggaaagat aaagcgggac gtagaaacta actgtgttgc caaatcgctg acgggtcaga
    14221 cgctgcgcct gttgggccag ttgttccaga tagccactgg cggcaggcga taacagcgcc
    14281 atcatgtcgt cgcgggtgag ttgcgaggca tttagcgccc gctctacgtc agcagccgtt
    14341 ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt gtcgccagcg attgctgaag
    14401 gttttcatgc cgatgcctcc agaaatccgg tcagcgggct ggtggcatga gcaaaataac
    14461 tgcggctgcc cggtccggac tgacgtgcca gcaggcctac ttctaccgcc agacgaaatg
    14521 ccttcgccat gttgacggga tcgtccgcga cggcaatcgc cgtattcact aacaccgcgt
    14581 cggcccccat ttccagcgcc tgcgtggcat ggctgggaac gccgatgcca gcatcgacaa
    14641 ccaccggaac tgtggcctgc tggataataa tctccagcat ggcacgggtt tccagtccct
    14701 gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc acagccgact tcttccagac
    14761 gtttacacaa taccggatcg gccccgcagt aaggcagcac gacaaatccc tgttgtacca
    14821 gcgtttcggc ggctttcagg gtttcgatgg gatcgggcaa cagccagcgg gcgtcagggt
    14881 gaatctccaa ttttagccag tttgtgccta acgcttcacg tgccagatgg gcggcgaaaa
    14941 tggcttcttc cgctgttttc gccccggaag tattcggcag cagggccaca cccgccgcga
    15001 taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc gacacgtttc atcgccagtg
    15061 tcaccagctg gctgccggaa gcgcggatcg cctccaccat cagttgtgaa gaagcgaatt
    15121 tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc cgcaatacgt aacatctcaa
    15181 ccccctgcaa taacctgaaa aagcaggatc tggtcgccat cctgcacgat atgttgcgtc
    15241 cactgctcac gcgggacgat ttgctgatta atcgccagag ccgcgcccgc ttgtcgttgg
    15301 tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg cgcactgcat cggttgatcg
    15361 ttaaacagga tctgcattgc ttcctccgca taccgggcaa ccactggcgc ggcgcaacgc
    15421 caggctgcgc cactggctcg acttaccgtc gaacagtcgg agttctcccg caggcgtctc
    15481 tataccgctt aataacttaa tggcttccag tgcctgcaaa gtgcccatca ccccgaccac
    15541 cgggccaacc acgcccgccg tgcggcagtt gcgttctggc tgctggttat ctggccacag
    15601 gcagcggtaa cacccctgct cccagggcgg tgtcagtacc atcaactgac cgccaaatcc
    15661 gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg caggcggcat taatctcctg
    15721 gcgagtcgcc atattgtcgg tacagtcgag caccacatcg gcttgtgcaa ccgcatcttt
    15781 caacgcctca cccgttaacc gttgttgtaa tgctgtcagt tgaatgtcgg gattcaactg
    15841 tgtcagtcgc tgttggctga cctgcgattt cgggctgttg atatcttcag tggtaaagag
    15901 gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca tctgccagta ccagcgtccc
    15961 gacgccagcc cctgccaggt atagcgcagc aggtgtaccc agcccgccaa gaccaataat
    16021 cagcacctgg ctgtcgagca gtttttgctg cccgtcaaga gcgatatcgt cgagcagaat
    16081 ttggcggcta taacgcataa agtcacggtc attcatcgcc aactcctgca atttccagca
    16141 actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat ggcgctgacg acggcgatac
    16201 tgccgacacc cgttgctatc accgcaggcg cgcgtgccag actgataccg ccaatcgcca
    16261 cggtgggata atccgccagg cgctcaacat gccgtgccaa ctgttccagc ccctgcggtg
    16321 cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc cagcgcgata taagaggggc
    16381 gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt cgaaacgccc agccgcaggc
    16441 ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc ttcctgcccc aaatggacgc
    16501 catacgcctg atgcttgatc gcgagccgcc agtaatcgtt gataaacaat cgcgcgttat
    16561 agcggcggcc cagcgcaatt gccgccacga catcggcttc cacctcttca tcgcgccgat
    16621 ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa cagacgttcg atccactgta
    16681 cgctgtccac caccgggtac agtcctaaac gaaaaggtac aggaggaaaa tcaggctgat
    16741 acatcacgct tcctccttat gcaggtagat ttctccgcct ctggcacgga agttctccga
    16801 catatccgcc attcccactt caatggtttg cgcggcggcg taatcgcgta cttcctggct
    16861 gattttcatc gagcagaatt ttggcccaca catggagcaa aaatgggcga ctttgccgga
    16921 ctcttggggc agggtttcat cgtgataagc gcgggcggta aacgggtcga gggccagatt
    16981 aaactggtct tcccagcgaa attcgaagcg ggctttcgac atggcgttat cgcgaatttg
    17041 cgcgcccgga tgcccttttg ccaggtcagc agcgtgggcg gcaatcttat aagtgataag
    17101 cccctgctta acatcttctt tattgggcag acccagatgc tcttttggcg ttacgtaaca
    17161 gagcatcgcg cagccaaacc agccaatcat cgccgcacca atccccgacg tgaagtggtc
    17221 atagcccggt gcaatatcgg tagttagcgg ccccagagtg taaaacggcg cttcgtggca
    17281 gtgctctaac tcctcggtca tattgcggcg gatcatctgc atcggcacat ggcctgggcc
    17341 ttcaatcatc acctgtacgt catattccca ggcgattttg gtcagttcgc ccagcgtatg
    17401 caattcggca aactgcgctt catcgttggc gtcctgaata gaaccggggc gcagaccgtc
    17461 gcccagcgac agcgaaacgt cataagcggc acagatttca caaatttcgc ggaagtgttg
    17521 atagaggaaa ttttcctgat gatgggagag gcaccatttc gccataatcg aaccgccgcg
    17581 agagacgata ccggtcaggc gtttcgcggt catcggcaca tagcgcagca gtacgcccgc
    17641 atggatagtg aagtaatcca caccttgctc ggcctgttcc agcagcgtgt cgcggaacgc
    17701 ttcccaggta agatcttcgg cgatcccgtt aaccttctcc agcgcctggt agatcggcac
    17761 tgtaccgatc ggcaccggac tgttacgcaa aatccactcg cgggtttcgt gaatatagcg
    17821 accggtggag agatccatca ccgtatccgc tccccagcgc gtggaccata ccagcttttc
    17881 cacttcttct tcgatggaag aggtgaccgc cgagttgccg atattggcgt taacttttac
    17941 caggaagtta cggccaataa tcatcggctc cgattccgga tgattaatgt tggccgggat
    18001 aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc gcagtgatat tttccggcag
    18061 atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa acctcgctac ggatgcgctc
    18121 gcggcccata ttctcgcgga tggcgatgaa ttccatttcc ggcgtgatga tgccctggcg
    18181 ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg cgttttggcg ttaataagcc
    18241 gctaaaacgt agctcgtcga gaccatcatc tgccagccgc gttctggtgt aatcggaact
    18301 gcgcacggta agttcttcgg tatcggcgcg cgcatcgatc cacggctggc gtagttttgc
    18361 cagcccttgc tgcacgttaa tggcgatctg cggatcacca tacgggccgg aggtgtcgta
    18421 gaccggaatc gcttcgtttt cttcgtactg cggctgttct ttgctaccgc caattagcgt
    18481 cgggctaagc tggatctcac gcatcggcac gcgcacgccg gggtgtgtgc cagtgatata
    18541 aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg tcgataaaat gttgggcccg
    18601 ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata gctcattcca aaaagttaag
    18661 gacgtggctt gtcagacgac ggatgaagca agagacgatc gccgcaaagg cgatgcgata
    18721 gcagattaac tcttgttccc ttcgcaggta ttagcctgat caggttccgc ggatcccgaa
    18781 taaacggtct cagccagtta aggcactccg acaagaaatt agcccccaca aacggggcgt
    18841 tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag gatgtttgac gcgggcggcg
    18901 tcaagcacca gcagaatcag cttatcttcc agcacaaagc gcgcttccag cgcctcgccg
    18961 atgtcagata aaacctgttg aaactcaagg taattatcat gatcgatggc ggtttccagg
    19021 ctggaatcgt agtaatccat aatctgttgg gtattggctt cgagttgcgg ccaaatcttc
    19081 gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa gaatgcgctc ataaatactg
    19141 aaatgtccgg cagacaagta atcgaccagg ctctgacaaa aatcatcaag ggctttttcg
    19201 tttagcctca tgtacgattc tttgccaggc ttaatgccaa ccagattgta gtaagccacg
    19261 agcagatgct tacgtacatg tagccagcga tcaaccagtt tgttacttcc tctgacgcgc
    19321 tccgtcaggt tatcgagctg gttaagcatg attgactccg caagtttgta ttcaaaaact
    19381 gctcagtgag aaatgtaaaa accatgttaa acatgccagt gatgcaaagg tagtgcaaga
    19441 gctatggatc gtataattga aaaattagat cacggctggt gggtcgtcag ccatgaacaa
    19501 aaattatggt tgccgaaggg agaattgcca tatggcgaag cggcaaattt cgatcttgtg
    19561 ggtcagcgcg cactacaaat cggcgaatgg cagggggaac ctgtttggtt aatacaacag
    19621 cagcggcgtc acgatatggg gtcggtacgt caggtcattg atctcgatgt tgggctgttt
    19681 caactggccg gacgtggcgt gcaactggcg gagttttacc gatcgcataa atactgtggt
    19741 tactgcgggc atgaaatgta tccgagcaaa accgaatggg cgatgctgtg tagccattgc
    19801 cgtgagcgtt actacccgca aatcgccccc tgcattattg ttgccatccg tcgcgatgat
    19861 tcgctcctcc tcgcccagca tacccgccat cgtaacggtg tccatacagt acttgccgga
    19921 ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc gggaagtgat ggaagagagc
    19981 ggaattaaag ttaaaaactt gcgttacgtg acttctcagc cgtggccgtt tcctcagtct
    20041 ttaatgaccg cgtttatggc ggaatatgac agcggcgaca tcgtgatcga cccgaaagaa
    20101 ttgctcgagg cgaactggta tcgctatgac gatttgccgt tactcccgcc gcccggcacc
    20161 gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc gggcagagta tgagtgatga
    20221 tacactgacc gcctgacgca ctaaggaaca gccaaaatga ccgaacttaa aaacgatcgt
    20281 tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc cagtatggat gatgcgccag
    20341 gcgggtcgct atctaccgga atataaagcc acgcgcgccc aggcgggcga ttttatgtcg
    20401 ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc aaccgctgcg tcgctacccg
    20461 ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc cggacgcgat ggggttaggg
    20521 ctctattttg aagccggaga aggtccgcgt tttacctcgc cagtcacctg caaagccgac
    20581 gtcgataaac tgccaattcc ggacccggaa gatgagctgg gttacgtgat gaacgcggtg
    20641 cgtaccattc gtcacgaact gaaaggcgaa gtgccgctga ttggtttttc cggcagcccg
    20701 tggacgctgg caacctacat ggtggaaggc ggcagcagca aagccttcac cgtgatcaaa
    20761 aaaatgatgt atgccgatcc gcaggcgctg cacgctctgc tcgataaact ggcgaaaagc
    20821 gtcactttgt atctgaatgc gcagattaaa gccggtgctc aggcagtgat gattttcgac
    20881 acctggggcg gcgtgcttac cgggcgcgat tatcaacagt tctcgctcta ttacatgcat
    20941 aaaattgttg atggtttact gcgtgaaaac gacggtcgcc gcgtgccagt cacgctgttt
    21001 accaaaggcg gcggacagtg gctggaagcc atggctgaaa ccggttgcga tgcgctgggc
    21061 ctcgactgga caacggacat cgccgatgcg cgccgccgtg tgggcaataa agtcgcgttg
    21121 cagggtaata tggatccgtc gatgctgtac gcgccgcctg cccgcattga agaagaagta
    21181 gcgactatac ttgcaggttt cggtcacggc gaaggtcatg tctttaacct tggtcacggc
    21241 attcatcagg atgtgccgcc agaacatgct ggcgtgttcg tggaggcagt gcatcgactg
    21301 tctgaacagt atcaccgcta agagcctgat ccatcaggct attttatttg ccattttgag
    21361 gccgggcagt gctcaaaatc ctcacgtact acgtgtacgc tccggttttt ccgcgctgtc
    21421 cggcctcaaa ctgccttcaa caattacgcc tgttgaacca ggctctaagg agcgattatg
    21481 gatctcgcgt cattacgcgc tcaacaaatc gaactggctt cttctgtgat ctgcgaggat
    21541 cgactcgata aagatccacc ggatctgatc gccggagccg atgtcgggtt tgagcagggc
    21601 ggagaagtga cgcgagcggc gatggtgctg ctgaaatatc cctcgcttga gctggtggag
    21661 tataaagttg cccgcatcgc caccaccatg ccttacattc caggttttct ttccttccgc
    21721 gaatatcctg cgctgctggc agcgtgggag atgctgtcgc aaaagccgga tttagtgttt
    21781 gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg tcgccagcca ttttggctta
    21841 ttggtggatg tgccgaccat tggcgtggcg aaaaaacggc tctgcggtaa attcgaaccg
    21901 ctctccagca aaccgggcgc gctggcccca ctgatggata aaggcgagca gctggcctgg
    21961 gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg ctaccggcca tcgggtcagc
    22021 gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag gctatcgtct gccggagcca
    22081 acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt tcgtgcgcta tacagcaaat
    22141 cagccctaat tcaggtaaac tgcggataat ttccgtattt gagaactcaa catgttacaa
    22201 aacccaattc atctgcgtct ggagcgcctg gaaagctggc agcatgtcac tttcatggct
    22261 tgcttatgcg aacgcatgta ccccaattac gccatgttct gccagcaaac cggttttggt
    22321 gatgggcaaa tttaccgtcg tattctcgat ctcatctggg aaacgctgac cgttaaagac
    22381 gcaaaagtaa atttcgacag ccaactggag aaatttgaag aagcgattcc ttcagccgac
    22441 gatttcgatc tgtacggcgt ttatccggca atcgatgcct gcgtggcgtt aagtgaactg
    22501 gtccattcgc gtttgagtgg tgaaacgctc gaacacgcgg tggaagtgag taagacctcc
    22561 atcacgaccg ttgcgatgct ggaaatgact caggctggtc gcgaaatggg cgatgaagag
    22621 cttaaagaaa acccagctgt agagcaagaa tgggacattc agtgggaaat attccgactt
    22681 ttagctgagt gcgaagaacg cgatatcgag ctgataaaag gccttagggc agacctgcgt
    22741 gagtcgggtg agagcaatat tggtataatt tttcagcaat aagaccagaa aacgtgattt
    22801 aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc cccgtctggt ctacatttgg
    22861 ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag agtgctattc tggcatttcc
    22921 gtcgcactcg atgcttagca agcgataaac acattgtaag gataacttat gaacaagact
    22981 caactgattg atgtaattgc agagaaagca gaactgtcca aaacccaggc taaagctgct
    23041 ctggagtcca ctctggctgc aattactgag tctctgaaag aaggcgatgc tgtacaactg
    23101 gttggtttcg gtaccttcaa agtgaaccat cgcgctgagc gtactggccg caacccgcag
    23161 accggtaaag aaatcaaaat tgccgcagct aacgtaccgg catttgtttc tggcaaggca
    23221 ctgaaagacg cagttaagta agattgcgtg gcagtgaaca gttttaacga aggggtggtt
    23281 tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg caggtgcgct gtttctcact
    23341 gcctgtagcc acaactcttc acttcctccc tttaccgcca gtggatttgc tgaagaccag
    23401 ggcgcggtac gcatctggcg aaaagacagc ggcgataatg tgcatctgct tgccgtgttt
    23461 agcccgtggc gcagtggcga taccacgacg cgagagtatc gctggcaggg cgataacctc
    23521 acgctcatca atatcaatgt ttacagcaaa ccgccggtga acattcgcgc gcgttttgac
    23581 gatcgcggcg atctgagctt tatgcaacgt gaatccgatg gggaaaagca gcagctttct
    23641 aacgaccaaa tcgatttata ccgttatcgt gctgatcaga tccgccagat tagcgatgcc
    23701 ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc atgcgatgga acagaccgtg
    23761 accacctgcg aagggcaaac cattaaacct gatttagatt cgcaggcgat agcgcatatc
    23821 gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg catggctgga agcgcccgaa
    23881 ggttcgcaat tactgttagt ggcaaactct gatttctgtc gctggcaacc caacgagaaa
    23941 acgttctgat ttaccagtgg cccataccca tatgaccgcc accaccgcag ccgccgtagc
    24001 ccatgcccat tccggctccg cgcggaatac ccgcttcagc catcgcgata tctcgtttca
    24061 cccgtaactc atctaacgac tgacgcaaat tctccatctc tttggcgacc gcgttaattt
    24121 tgctgctatc cggtgggttc gcggctaaca gggcattgta ttcataacgc ttcgtcacca
    24181 gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt atggattttc tgccacgctg
    24241 tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca cataccgtgt ccgccgtgag
    24301 caaatgcaga tgtcgatccc atcgccattg ctgaaagcgc catcattacc agggcaattt
    24361 tcgtgttccg tttcatggtt aatcctccag tggttgtctt acttcgggta ttgcatcttc
    24421 cgtgccaacg atgaaacgct gatatgacgg gtaatctggc atgataaacg agtaaaaatg
    24481 actcgcctgc tgcgggtagc gagtcatttt tactcattga aacttaagcc tttgtgttac
    24541 agcgcagggt aagcgctgat aaaagatggc atgatttctg ctgtcagaaa gggatgagca
    24601 ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac tccttagcta aatggttaag
    24661 cgcgatcctc cccgtggtca ttgttgggct ggtagggttg tttgcggtga cggtgattcg
    24721 cgattatggg cgcgagactg ccgccgccag acaaacgctg ctggaaaaag gcagtgtact
    24781 tattcgcgct cttgaatccg gctcgcgcgt cggcatgggg atgcgcatgc atcatgcgca
    24841 gcagcaggcc ttactggaag aaatggccgg gcagcctgga gtacgttggt ttgcggtcac
    24901 ggatgaacaa ggaacaatcg tgatgcatag caactccggc atggtgggaa aacagcttta
    24961 ttcccctcag gaaatgcagc agttacatcc gggagatgaa gaagcgtggc ggcggatcga
    25021 tagcgcagac ggcgagcctg ttctggaaat ttatcgccag tttcaaccga tgtttgctgc
    25081 tggaatgtac cggatgcgcc atatgcagca gtatgccgcg acaccacaag caattttcat
    25141 cgctttcgac gccagtaata ttgtgagtgc cgaagatcgt gagcagagaa acaccctgat
    25201 tattctcttc gccctggcga cggtcttgct ggcaagcgta ttgtcattct tctggtatcg
    25261 ccgctatctg cgctcgcgcc agcttctaca agatgaaatg aagcgcaaag agaagctggt
    25321 ggcgctgggg catcttgcgg caggcgttgc ccacgaaatc cgtaacccac tttcctcgat
    25381 taaaggactg gcgaaatact ttgccgagcg cgcgcctgca gggggagaag cgcatcaact
    25441 ggcgcaggtg atggcgaaag aggccgaccg tttaaaccgc gtggtaagcg agttgctgga
    25501 actggttaag ccaacgcatc tggctttgca ggcggtggat ctcaacacgc tgattaacca
    25561 ctcattacag ctggtaagcc aggatgcaaa cagccgggag atccagttac gctttaccgc
    25621 caacgacaca ttaccggaaa ttcaggccga tccggacagg ctgactcagg tcctgttgaa
    25681 tctctatctc aatgctattc aggcgattgg tcagcatggc gtgattagcg tgacggccag
    25741 cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc ggtaagggaa ttgcggcaga
    25801 tcagcttgaa gccatcttca ctccgtactt caccaccaaa gccgaaggca ccggattggg
    25861 gctggcggtc gtgcataata ttgttgaaca acacggtggt acaattcagg tcgcaagcca
    25921 ggagggaaaa ggctcaacgt tcaccctctg gcttccggtc aatattacgc gtaaggaccc
    25981 acaaggatga cgcacgataa tatcgatatt ctggtggtgg atgatgacat tagccactgc
    26041 actattttgc aggctttact gcgcggctgg ggctataacg tcgcgctggc gaacagcggg
    26101 cgacaggcgc tggagcaggt gcgggaacag gtttttgatc ttgtgctttg cgatgtgcga
    26161 atggcggaga tggacggcat cgccacgctg aaagagatca aagcgttaaa cccggcaatt
    26221 ccggtgctga ttatgactgc gtactccagc gtcgagacgg cggtagaggc actgaaaact
    26281 ggggcgcagg attatctcat caagccgctg gatttcgata acctacaggc gacgctggaa
    26341 aaggcgctcg cgcatacgca cagtattgat gctgaaacac ctgcggtgac tgccagccag
    26401 ttcggtatgg tcggtaaaag cccggcgatg caacacctgc tcagtgaaat cgccctcgtc
    26461 gcgccatcgg aagccacggt gctgatccac ggcgattccg gcaccggtaa agagctggtc
    26521 gccagggcga ttcacgccag tagcgcacgt agcgaaaaac cgctggtaac gctcaactgt
    26581 gcggcactca acgaatcctt gctggaatct gaattgttcg gtcacgaaaa aggggcgttt
    26641 actggagccg ataaacgccg ggaagggcgc tttgttgagg cggacggcgg cacgctgttt
    26701 ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc gtctgctgcg tgcgattcag
    26761 gagcgcgaag ttcagcgtgt cggtagcaac cagactatct cggttgatgt ccggctgatt
    26821 gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg ggcgttttcg ccaggatctc
    26881 tactatcgcc tgaatgtggt ggcgattgaa gtaccatcgc tgcggcaacg gcgggaagat
    26941 atccctctgc tggctggcca ttttcttcag cgctttgccg agcgtaatcg caaggcggta
    27001 aaaggcttta cgccacaggc gatggatttg ctgatccact acgactggcc gggaaatatt
    27061 cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc tgaccgggga atatatttcc
    27121 gaacgcgagc tgccgctggc gattgccagt acgccgatcc cgctggggca aagtcaggat
    27181 attcagccgc tggtggaagt ggaaaaagag gtgattctgg cggcgctgga gaaaacgggc
    27241 ggcaacaaaa ccgaagccgc ccgtcagtta gggatcacgc gcaaaacgct attggcaaaa
    27301 ctgtcgcgtt agttctgctc gcgttcgatg gcgcgccaac cgatatcttt ccggcagaag
    27361 caatcatccc agtggatatc ggtcattaag gcgtaggcgc gtttctgcgc ttctgccacg
    27421 gtatgaccca gcgcggtgac gcacagtacg cgcccgccgc tggttaccac ctgctcgtca
    27481 tccgccagtt ttgtgcccgc gtggaacact ttgccgtcag ccacttcttc cagcggcagg
    27541 ccgtggatca catcgccggt gcggtaatcg cccggatatc cacccgcagc catcaccacg
    27601 ctgagagaag cacgttcatc ccactctgac gttttctcgt ccagtttgcc ttcacaggcc
    27661 gccaggcaga gctcaaccag atcggacttc atgcgcagca taatcggctg ggtttccgga
    27721 tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc cctgtttgtc gatcatcagg
    27781 cccgcataga gaaaaccggt gtaggtgttg ccttccgccg ccatgccttt cacggttggc
    27841 cagatgatac gttccatggt gcgctgatga acttcatcgg ttactaccgg cgcgggggag
    27901 taagcgccca tcccgccggt gtttggtccg ttatctttat cgcctacgcg tttgtgatcc
    27961 tggctggtag ccatcggcag cacatgctcg ccgtccacca tcacgataaa gctcgcttct
    28021 tcgccatcga ggaactcttc gataacgatg cgatgacccg cgtcgccaaa agcgttgccc
    28081 gccagcatat cgtgaacagc cgcttccgct tcttccagcg tcatcgccac gataacgcct
    28141 ttcccggcag ccagaccgtc cgctttaatg acgattggcg cgcctttctc acgcagatac
    28201 accagcgcag gttctacctc ggtgaagttc tggtattccg ccgtagggat cttatggcgg
    28261 gccaggaaat ctttggtaaa cgcttttgag ccttccagtt gggccgcacc tgcggttggg
    28321 ccgaagattt tcagcccggc ggcgcggaag gtatcgacca cgcctttcac cagcggcgct
    28381 tccgggccga cgatggtcag atcaatcttt tcgttttgtg cgaaatccaa cagcgccggg
    28441 atatcggtca cgccgatagc aacgttttgc agcgcgggtt ccagcgcagt gcctgcatta
    28501 cccggagcag caaaaacagt ctcaaccagc ggcgactggg ccgctttcca ggccagcgcg
    28561 tgctcgcgcc cgccgttacc aatcactaat actttcatct attgctccat taatggcgga
    28621 agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc gtcggcggcg gcaatcactt
    28681 cgtcatcacg gatggaaccg ccaggctgga ttacgcaggt cacgcccgca gcggcggcgg
    28741 catcaatacc gtcgcggaac gggaagaacg cgtcagaagc catcgaggaa cctttcactt
    28801 ccaggccttc atcggcagct ttaataccgg cgattttcgc ggagtacacg cggctcatct
    28861 ggcccgcgcc aataccgatg gtcatattgt ttttggcata gacgatagca ttggatttca
    28921 cgaatttcgc cactttccag cagaacagcg catcacgcag ttcctgttcg gtcggctgac
    28981 gttgggtgac gacgcgcagt tcttccgcac cgaccatccc caggtcacga tcctgaacca
    29041 gcagaccgcc gttcacgcgt ttgaaatcaa gacccggaac acgctcgccc cactgaccgc
    29101 aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt cagggcttct tcgctggcgg
    29161 aaggcgcaat aatcacttcg acaaactgac gagaaatgat ggcctgtgcg gtttccgcat
    29221 ccagctcgcg gttaaaggca atgatgccgc cgaatgcgga ggttgggtcg gttttgtacg
    29281 cgcgatcgta agcatcaaga atggaattgc cgatagctac accgcaaggg ttggcgtgct
    29341 tcacaatcac acatgccggc tcggcgaact ctttcacgca ctccagcgcc gcatcggtat
    29401 cggcgatgtt gttataagag agggctttac cctgaacctg ggttgcggta gcaacggagg
    29461 cttctttcac attctcttct atatagaagg cagcctgctg gtggctgttc tcgccgtaac
    29521 gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg tgggaagcga ccggcggctt
    29581 ctttgctttc accgtggtac gccggaacca tgctgccgaa gtagttggca atcatgctgt
    29641 cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc gaaacgggtt gcaagcgtca
    29701 gcgatccttc gttgtcatcc atctctttaa taatggcgtc gtagtcgctg ctcttcacca
    29761 cgattgcgac atctttatgg ttcttagcgg cggagcgcac catggtcggg ccgccgatat
    29821 cgatgttctc aaccgcatct tccagcgagc agccttcacg agcaacagtc tgggcgaacg
    29881 gatacaggtt aacaaccacc atatcgatag gctggatctg atgttcttcc ataatggcat
    29941 cgtcctggcc gcgacggccc agaatgccac catgtacttt cggatgcagg gtcttcacgc
    30001 gtccatccat catctccggg aaaccggtgt aatcggaaac ttcggttacc ggcagacctt
    30061 tttctgctaa cagacgggca gtgccccctg tagacagcag ctccacaccg cgtgcggaaa
    30121 gtgcctgggc gaattcgacg ataccggctt tgtcagaaac actgagcaga gcgcggcgga
    30181 ctggacgacg ttgttgcatg gtaaatcccc tgggtttgac tattacagag agcgttagct
    30241 gaatttttcg cgaaaaactc agctaacgcc cctaacgggg catccttatt tttcgcccgc
    30301 attgtaacga aaacgtttgc gcaacgctcg cgaatttttc tctttcaatg gtgatcacaa
    30361 ttttgacagt ggttaccgtg ggaaaaatac agaaattaca ttgatgattg tggataactc
    30421 tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat gcagcagtca gtcatttttc
    30481 tgcaattttt ctattgcggc ctgcggagaa ctccctataa tgcgccttca tcgacacggc
    30541 ggatgtgaat cacttcacac aaacagccgg ttcggttgaa gagaaaaatc ctgaaattca
    30601 gggttgactc tgaaagagga aagcgtaata tacgccacct cgcgacagtg cgctaaagcg
    30661 cgtcgc
//
LOCUS       DAETQL010000045        30297 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.045, whole genome shotgun sequence.
ACCESSION   DAETQL010000045 DAETQL010000000
VERSION     DAETQL010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30297
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.045"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="K0W65_002227"
     CDS             complement(<1..816)
                     /gene="wcaD"
                     /locus_tag="K0W65_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBI3095481.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYILIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="K0W65_002228"
     CDS             complement(791..2008)
                     /gene="wcaC"
                     /locus_tag="K0W65_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBI3095482.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="K0W65_002229"
     CDS             complement(2005..2493)
                     /gene="wcaB"
                     /locus_tag="K0W65_002229"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBI3095483.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="K0W65_002230"
                     /pseudo
     CDS             complement(2496..3335)
                     /gene="wcaA"
                     /locus_tag="K0W65_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="K0W65_002231"
     CDS             complement(3428..5590)
                     /gene="wzc"
                     /locus_tag="K0W65_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBI3095484.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="K0W65_002232"
     CDS             complement(5593..6036)
                     /gene="wzb"
                     /locus_tag="K0W65_002232"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBI3095485.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6042..7181)
                     /gene="wza"
                     /locus_tag="K0W65_002233"
     CDS             complement(6042..7181)
                     /gene="wza"
                     /locus_tag="K0W65_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HBI3095486.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7490..7639
                     /locus_tag="K0W65_002234"
     CDS             7490..7639
                     /locus_tag="K0W65_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095487.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7840..9423
                     /locus_tag="K0W65_002235"
     CDS             7840..9423
                     /locus_tag="K0W65_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBI3095488.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="K0W65_002236"
     CDS             complement(9875..11728)
                     /gene="asmA"
                     /locus_tag="K0W65_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBI3095489.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="K0W65_002237"
     CDS             complement(11750..12331)
                     /gene="dcd"
                     /locus_tag="K0W65_002237"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBI3095490.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12423..13064)
                     /gene="udk"
                     /locus_tag="K0W65_002238"
     CDS             complement(12423..13064)
                     /gene="udk"
                     /locus_tag="K0W65_002238"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBI3095491.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13382..13624
                     /locus_tag="K0W65_002239"
                     /pseudo
     CDS             13382..13624
                     /locus_tag="K0W65_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13623..14564
                     /gene="mdtA"
                     /locus_tag="K0W65_002240"
                     /pseudo
     CDS             13623..14564
                     /gene="mdtA"
                     /locus_tag="K0W65_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14564..17686
                     /gene="mdtB"
                     /locus_tag="K0W65_002241"
                     /pseudo
     CDS             14564..17686
                     /gene="mdtB"
                     /locus_tag="K0W65_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17687..20752
                     /gene="mdtC"
                     /locus_tag="K0W65_002242"
     CDS             17687..20752
                     /gene="mdtC"
                     /locus_tag="K0W65_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBI3095492.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20753..22168
                     /locus_tag="K0W65_002243"
     CDS             20753..22168
                     /locus_tag="K0W65_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3095493.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22165..23568
                     /gene="baeS"
                     /locus_tag="K0W65_002244"
     CDS             22165..23568
                     /gene="baeS"
                     /locus_tag="K0W65_002244"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBI3095494.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23565..24287
                     /gene="baeR"
                     /locus_tag="K0W65_002245"
     CDS             23565..24287
                     /gene="baeR"
                     /locus_tag="K0W65_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBI3095495.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24478..24810
                     /locus_tag="K0W65_002246"
     CDS             24478..24810
                     /locus_tag="K0W65_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBI3095496.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24957..26318
                     /gene="yegQ"
                     /locus_tag="K0W65_002247"
     CDS             24957..26318
                     /gene="yegQ"
                     /locus_tag="K0W65_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBI3095497.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26660..26977)
                     /locus_tag="K0W65_002248"
     CDS             complement(26660..26977)
                     /locus_tag="K0W65_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095498.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27383..28282
                     /gene="yegS"
                     /locus_tag="K0W65_002249"
     CDS             27383..28282
                     /gene="yegS"
                     /locus_tag="K0W65_002249"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBI3095499.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28364..29137)
                     /locus_tag="K0W65_002250"
     CDS             complement(28364..29137)
                     /locus_tag="K0W65_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI3095500.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29243..30283)
                     /gene="gatD"
                     /locus_tag="K0W65_002251"
     CDS             complement(29243..30283)
                     /gene="gatD"
                     /locus_tag="K0W65_002251"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBI3095501.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7012 a         8046 c         7829 g         7410 t
ORIGIN      
        1 acctaaatcg cccagacggg tggaaatata aggaaacgcg attaccacac caacccggaa
       61 tacagccaga gaaatcaatg ctaacggtaa ttttttctta atcgcctctt tattcagata
      121 ttgaaatgcc cactccagca aataaaacag gataaaggtc ataacccctg aaaacgatcc
      181 ggataatatt atccctgcga gaatcagggc atctgtttta ggcgttttga taccaaactg
      241 tttgatgctg agccaaattg agattaatgc cagagcgaaa aatgccggtt cgaaataaag
      301 tgctgtcgtg cgcttgccgc cgaatttaat gaaattcagc acatagctgt tgctgtaaat
      361 cagatatttc gaaatcgact ccataatact gctgccaccg gtgaggataa tttgcgccat
      421 ctccaccgcc gccagcgcca ccaccagtcc gaccaccaga taaaagaaac gtaatatctt
      481 acgatggttg tgtggcgaaa ttgttttaaa acgaatactc cacaccatgc caataatgat
      541 caaaatatag acaaacagca tagttgaagt gacgtattta ccggcatcca acgactgacc
      601 aaacaggtag ttaaacgccg tgagtcccgc gccaatccct aaggcaatca tcaatttttt
      661 aatgctgatg cgttctaaaa acagcagtaa caagacaggt aaaaaagtga cgatggtgat
      721 ggggaaactt tcgccaagct gggcgatttt gacgttaacc agcaggtaga tcagtggcag
      781 cagcaggtag ctacagattc tgatagaagt tgacatactc ctccagcatc tgttgtccac
      841 tgtaggcggc gcgactgcgt tggctgaact cagccagcgt ggtaccaaat atcgcctgcg
      901 cgatttccgg tttgcttaac tgtaccagtt gcagcacctc ttcttcactg acggttttac
      961 caccggattt ttgcagcact tcccgcgccg catcgctatg ggtggcaatc accggcacgc
     1021 caatcgatag cgcctcacac aaaatcagcg ggtagttatc gacacgagaa ctgaacacca
     1081 gcgcatccat ctgattgagc gcgctcatca acttgcgctt gtcagtttca aagccgtgat
     1141 taaccacgtt gccagcggtg aacggcgaga acttaccaaa ggtatgcagt tcgattttgt
     1201 cgcccagcgc catcatctca cgcaccagtt gctggttagt tttgccgtcg taacgcaggt
     1261 catgcgccac cactgcgatt ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg
     1321 ccagaatcgc ttcggttgcc atatcaatgc cattattgat aatccggcaa cgccctggcc
     1381 cgtacagact attgaaagcg tcagccacat gctggctggg ggaaataaac tgacagccca
     1441 gcgccagcat ctcgcggaat aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa
     1501 tcttcaccgg cggatagtta tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt
     1561 cgcaaccgtc ggtaaaggcg cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga
     1621 ccagggtgac atccggttta tggtttttca ctttttcgca aaagcgcacc acgctgttaa
     1681 gattcaacca gtagctgtgc agcacatgaa aatgcaggac caccggaccc ggtgtgcgag
     1741 taatggtgcg atataactca ttgaaattgc cgaacagatc gcgattaaac agacgaaaca
     1801 acgcaatgtt cgccatcgcg gtcatccgcg gcgtatgttt gatgacctgc ggatagttct
     1861 gatggctgac gctctctttg ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc
     1921 cctgttgcag cgcacgctgg tggagatcta acgccacacc tgctgccccg ccttccgcca
     1981 gtcgcacatt aaattgcaga atattcattt aatcaccttc actcgcgctt tttctcccac
     2041 caccagcgcg ttgtccggga cagagtcgag caccacgctg cccgcaccca cggtgacgtt
     2101 gttaccaagc gtgatatcac caagaataat gacgttggca ccgagttcga cgccgttgcc
     2161 aatgtgtgga catgccatgt tatcggcacc ccgattgccg atagtgacgc cgtggcgaat
     2221 ggtgaaatca tcccccgcca ccacgttttt attgatcact acggcgtaac cgtgatggat
     2281 agtaaagcgg cggccaatgg tcgcggcggc ctggatttca taaccgaaaa agcattcggt
     2341 gataatgcga tacagcacca gcagcggggc cgcccacaga ttgttgagga cgttcttttt
     2401 gcgccacacc gaacaaaaat gagcgacgcg ataggcaagt accatgcagc acgggcgtaa
     2461 actccagctg ttggcgcgca gatcttccag catctttagc gcccccgaat accatcagcc
     2521 agacgtttgc cgttacgcac cgacagcagc gtcaacaagg tgcgccaggt catacgttta
     2581 ttgcggatct ggtagagggt aaacagctga tattttttac tggcgcggtc gaatttgtct
     2641 ttgtgcttgc ggtaaaagtg gaagtatccg gagaattttt tcggtgacga ggtgatctgc
     2701 atttcgccgt gattgatatg caggatctgc gtcgcctctt ccactttcca cggttcgccg
     2761 tactccacca ccatccgcag gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg
     2821 aacaaacact ctttgaaacg ccatgcccag gtaaagacct gattgccaat gatattacgt
     2881 ttatagaaca ggcggcgtga gtacggcgat ttgggataca gtggtaagct cgccggttgg
     2941 gaataaactt ctccctagca aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt
     3001 tgctgtttat gggcgaggaa gacgctcagg cggttgggcg tccattcatc atcatcatcg
     3061 atcccggtga tgtattcccc ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc
     3121 ccgctgttaa tgtcgttgtg gatgtaagtg atacgcggat cgttgagggc agtgacgtac
     3181 tgttgcagtt gttcccagga ggtggagcaa tcatccacga tgatcatctc ccagttgctg
     3241 tagtcctggc gcaggaccga ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt
     3301 ggcatataga ttgagatcag cggattgtct ttcataggtt gttctcccgg cttatgagct
     3361 ggtttgcttg ccggatgcgg cgtgagcgcc ttatccggcc tacggggcgg tgcgaatgca
     3421 ggcgtcgtta tttcgcatcc gacttatatt cgtattcgta atagccataa tcctgatacg
     3481 cgctggcgcg gcggaagatg gagttcagaa tcaccccttt caccggaata ccgttttgct
     3541 caaagcggct cagactggtt tccacttctt tcaatgtgtt gaccgcataa cgcgccacca
     3601 ttaacgtggt tccgacatga cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg
     3661 gcgtatcaat caacaccagg tcatagtttt tactcgccca gttcaccagt tcggcaaagc
     3721 gttcgctcat taacagttca gaaggatttg gcggcacctg accgcgcggg atcaggtcaa
     3781 atttggcaat agaggtcggt ttagcagcgg tagtaatatc gccctgacca atcagaatct
     3841 ccgacagccc attgacatta ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat
     3901 cgcagtcgat caacaacacg cgtttattgg tctggctgat caccgccgcc aggttggcgc
     3961 agacaaaggt tttaccgatt gacgggctaa ccccggtcat catcaacaca ttgttctgcg
     4021 cctgcatcat cgcgaagtgc agactggtac gcagactacg gatggcttca atcgccagat
     4081 cggttggatt ccccactgcc agtagctggc tctgtttata gcgtttaacc cctttgatgg
     4141 ttttgacgct atcgcgcgct ttctgccact ccgacagcgg gatgctggca tagacgctga
     4201 ttccgtgttc ttccagcact tgcgggcttt cgatgccgcg attaaacaac gagcgcagca
     4261 gcacacccac gatagagagc atcaggccaa ggataatcgc gccgaggata atcagccctt
     4321 tcttcggttt tagcacgcca ggctgggtga ttgccgggtc aataatgcgc acatcgccga
     4381 cggtgctggc ttcggtgatt ttcagctcct gctctttatt cagcagttgc atatagacct
     4441 gctgaccaga ctcgacatcg cgggtcagac ggacaatttc ctgctggttt ttcggcatcg
     4501 ccgttacgcg accgttaagt ttggctttct cttcttccag cgcctgacgt ttctccagca
     4561 gcgtgcggta cgccgggtga actttggtgt acagcttgga gatttccgcc tctttaaagg
     4621 tcagttcgtt caactgcgcg tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa
     4681 gcggcagatc aacagaatct ttatcctgac ggaaggcatt cagtttgttt tcggcaacat
     4741 cgaggcggct acgtacttcc ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt
     4801 ccgccgattt gcgctcaata ttttgttcct gatagttacg agcgatgctg ttaagaatgt
     4861 cgcggatctg ttcgcgatct tcaccggtat aagtcaggct cagtacgcct gcgtctttgc
     4921 cgttctccgt taccgtcagg ctgttttgca gttggttgat catccccaac gtggagtatt
     4981 tggtgacggt aaactcactg cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc
     5041 cttctttttt cagcatctgg cccgcttgcc cacgggcgct aaagccgcca tcactgctca
     5101 gggtgtagtt tttgtcatcc agcacattaa gcgtaaacac ctgatccgcc atctctttcg
     5161 ggcggttaaa ggtagtcact ttcaccgtct cgttctgacg ccccatcagg cgatcccagc
     5221 ccgccccgaa aatcgggaac gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca
     5281 ccgttttacc aagcaccaga cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg
     5341 gtttgttggc taatgccgag ccgatatcct gcaccagcga attgccgctg ttttgctcga
     5401 tttgtaccag tgcgtcggca ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac
     5461 agagggcaaa tacgacggtg atgccaatca cccaccagcg cgcttcaatg acggtgccga
     5521 ccaggcgacc aatatcgatt tcatcactgc ccgttaccgg agcggcatgt tgttttactt
     5581 tttctgtcat tcttatacct gctctgcgtt caatgcctgc gcccactggc gggcagaccg
     5641 ttcaagtaat gtgtacaccg ctgcaaacgt ttcccggctt ttgtgatacg gatcggggat
     5701 ttcacattcg ttatcccagt gaccaaacag catcactttg ccgcgcatct ccggtgccat
     5761 ctcgcataag cgttcgatat ggcgcttatc catggtcaaa atcaggtcat agttgcgaca
     5821 caggcggcgg ctgatttgtc gtgcacagtg gccttccaga gacagttgat gttctgcggc
     5881 gacgctgata gcggtaggat cagcgccctt accgaccagc gcgccgagtc cagcggactc
     5941 cactttcagt tccgggtgat aacgttgcag taagcgttcc gccatcgggg aacggcaaat
     6001 attgccgaca cagacaacta agatgttgtt aaacatgacg attaccagtt atgaatgtcg
     6061 ctggctgtat ccgtcatgta acggacaccg ctaatagttg gcagcaactg attgatcaga
     6121 cggttccagc gggaaaccgg ggcggtggtg acatacacca cgtcataagg ttgcaggcgg
     6181 aattctgtcg ccatcaccag cgacgtcgca tcggacatat ccagctggta gatattggca
     6241 atcttgccgt tacgcccgcc ctcgcctttc agcggacgaa tgacaaagat gccgctggcg
     6301 ttggaggtgg tcatgtcgat accttcggca ttggccagag cttcagtcag ggtcatgccg
     6361 ctaaagtcca ttttcagggt gctctgtttc ttcacttcac ccatcacaaa tactttcaga
     6421 tcatcattac gcggcacgta gagaatatcg ccgggataaa gcagtcggtt ctgattgagg
     6481 tcgccgtttt gcatcagcgc ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc
     6541 accacgttgc gccagtcagc ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga
     6601 atggtcagtg gtacgttggt gatcgcctgt tgaccggatt tattcacctg acctgagata
     6661 taggcctttt gtgagcggaa ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac
     6721 gtcgctaagc gcccggtaat atcactgcgg atttcagcga gcgttttccc gactacgtga
     6781 accttgccga tatacgggta aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg
     6841 ctggagctgc ggtactgacc agctggcgtg gtgagttccg ggtgatccca gacggtgaca
     6901 ttaagaacgt cccccggccc gacgcgatac tggtaattcg cgatctcgct ttccagcgtc
     6961 atattggggc gcgctacatt cgggcgtggg cgtaattggt caatcaggcg cggggtcagc
     7021 ggataaacat tcaccatttt gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct
     7081 ttgcccatcg tcgacatatt gctgcccgga agtactgtgc aaccgcttat caaggttact
     7141 gacaccaata atggcatcaa tttcattttg gatttcatca ttgtttattt atcactttgg
     7201 cagagtaatt atcctgtgca ctattaatag caatgtcgcc atgcacattt accttgcagt
     7261 taattgaata aaaatttaac tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta
     7321 ttgacagaat aattcagact ggtctttcag gcatccagac acgctaccgc ccctggcttt
     7381 ttagctacca atacactgat tgagcttaat ttttcaaacc ctctctgcat acaatcgttg
     7441 atgagaaagg gttattgcgg aaattaactt ccgaatataa ggtgacatta tggtaattga
     7501 atattggctc gccaataatg caggaggaag tgttacagct aacggaatag caggcaaggt
     7561 aacaattcgg caattggcta tttttaagaa ttatattaag tatcattcaa tatggttttt
     7621 aggagtttct ttaggttgac aatatttaat attgcgtctc cacatgcgat atttcttaaa
     7681 taatgtttta ttattaccgc ttttaattca gggataatgt gaggttatta ccctcaaata
     7741 atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca tgatcgaatt
     7801 gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat tgccgatccg
     7861 tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg cattgataac
     7921 ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg
     7981 gttaccgggc tgctgctggc gatgttaatg cgcctgttac tgctagcgtc aatctcctgg
     8041 ctggtcaccc tgactcaacc gctgttcagc ttccgctcgt ttacctttag cgcccgcgac
     8101 ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga gctgaacgaa
     8161 cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa gttctggggc
     8221 gtggtgacgc aaattgtggt actggacgcc attttctcac tcgactcagt gattaccgcc
     8281 gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc tatcagcctg
     8341 atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac catcgttatt
     8401 ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg tttcggcttc
     8461 gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat cgaggcactc
     8521 aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaatcagac gctacgccag
     8581 cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc ggaactggac
     8641 gccaaaaccg cgtccatgct ggtggatcat ggaaaccagc agatctttaa tccgcaggaa
     8701 cggcggatga ttgagcgggt acttaatctt aaccagcgta ccgtaagcag cattatgacg
     8761 tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagaagaaat ccgccagttg
     8821 ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgacgc agaagatttg
     8881 ctcggtgttg ttcacgttat cgacctgcta caacagtcac tgcgcggcga accgctcaac
     8941 ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt gttaccagcc
     9001 ctggagcagt tccgtaatgc ccgcacgcac tttgcttttg tggtggatga gtttggctcg
     9061 gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa cttaccgaac
     9121 gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg ttcctggacg
     9181 gcgaatggtc atatgccgct ggaggatctg gtgcaatatg tgccgctgcc gctggatgaa
     9241 aaacgtgaat atcacaccat tgccgggctg ttgatggagt atttgcagcg tattccaaaa
     9301 cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt ggaaagccat
     9361 cgcgtgcaga aggtgcagat tataccgctg cgtaaggatg gcgagatgga gtacgaggtg
     9421 tgatggtatc ggccatttta cccaatgctg cgtaacgcct tataacttca gcaatgaagc
     9481 gttgtctgtg gtaaacggac atcacagcgg gggtgtgggt ccggctgtaa atcgccgaat
     9541 cgtttcctgt aggctggggc agcccacatg gatgtgggct gagggcgcgt tttacaggga
     9601 tgttacctcg cgcccgtacc cgatagccga aaggaataag atgagggaac cgcgccagcg
     9661 gcgatttacc gccgggagcc cgggttgcca gggtggtggc gattgagcca ccctggcacg
     9721 ttcaccggct tgagcgttcc atagtagcga ggaacatcaa gtgaacggaa cagccttcga
     9781 tcttacatat tcagaatata atagtgccgc cgcgaacgcc tgatgcgacg ctttacgcgt
     9841 cttatcaggc ctacaggaca ggtcacctgg taacctacat cttctccagc aacttcttca
     9901 catctttgcc attgcgggaa tctttattcc gctcggccca gtcattcagg cgacgtttcg
     9961 cttcgtcctg tagatgtttg cgcagtaact gatccacttg caggctgtaa ttgagttgct
    10021 gccagttgcc ataaacccgc agcggtactg gcgtttcttt gaggaaatca atcagtttgc
    10081 tttccccgtt ccagccaccc acgacccgaa tatcaaactg ggtgtcgcag gtttgatctg
    10141 ccagattcaa catgccttcc cctgtcagcg ccagcacagg cgattgacct tgcatgtcgt
    10201 ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt aaagcggtca agccgcgtca
    10261 cgttatcgaa gttttcagcg gccttcacat caccgccatt acgctctacc gcttgctgaa
    10321 tcatctgctg gaagttcatc ccttccatac gtgtgtcggt catctcaaca tgcgcctgtc
    10381 cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc accggagaaa tcaccagcca
    10441 gtgacatttt tccggtcaac gaaatcggat agttaaacgc cttcagaatg gtaccaatct
    10501 caacgttctc cagccgtggc tggaagttta tccgcggatt tattgatgtc gcgtccagcg
    10561 tgcccggcag tgaaaccttt ccaccgttaa gcttgccctg cagttgagta atttccagca
    10621 aacccgactt gttggtcatt tgcgtggcaa catctgtaaa attcattccg cgccagcgca
    10681 cgttactggc ctgcaacaaa atatcagctg taaagccttg cagtccctga taggccggtt
    10741 catcaatacg cgaagaaatg accgggcgcg gcaacgttga ttggctctgc ccctgctgcg
    10801 cggcaccgtt ttcaccattc gcggtttcat taagcgggat gaggttgtca agattcagtt
    10861 gcgggaattg cagtctcagc tgccattccg gtttctctgt cagcgtgacc tgtgcttgtc
    10921 cgctcagcgt actgtcattg gcggtcaaac taatttggtt aaatgaaagg cgtttatgtg
    10981 actcctgcca ctgggactgg aaactcccct gcccctgaat accttgtttt ggtaaatcgg
    11041 caccctgcaa ctgccagtta atttgttcaa tagccgctgt taaatcatgc ggataatcag
    11101 aagcatctac cgtaccgtta agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc
    11161 cggagaactc aaatgagcca cgatgttggg gatcttgttc catttgcagg cggatattgc
    11221 ggattgtcac ctgctcgtca tcttcatgct ggaacaccag aacgctgtcc gccaccttaa
    11281 gactggatat atcaaacgac catccgcgat cgtctgacag atccggcaag gtattgtcgc
    11341 gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc agttggatca
    11401 ctgccccttt tagcatcacc tgcttaacgc tcagttgatg actcagtagt ggtaaaagcg
    11461 ccacgtccag acgcatgttg tcggcgcgaa ccagtggctg gcttgcgccc tgggcggtga
    11521 gagacattcg cccggagagg atactaagct gcggccagac gtgccaacgc agtggcccgt
    11581 cgagctgcaa ttgataaccg ctacgtgcag caacttgctt gaccatatag tcgcggaaat
    11641 cattcggatt caccagcaac actaacgcag ataacccggc caccagcacg accaagagta
    11701 tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat tagtctttat
    11761 cgattcggct ggctaccgcg ccctgctggt tgcgatattt cgcatcttca cggcggttgt
    11821 aaggtcgcgc cgccgggccg gaaagcggct caaagctcag cgcaccaatt aacatgcccg
    11881 gacgcagcgc cagcggcagc ttaccggagt tgtagaactc cagcacaatg caaccagacc
    11941 agcccggatc gatacggtgc gcggtgacgt gcaccatcag ccccagacgc gccaaagagg
    12001 aacgcccgtc cagccagccc accagatcgg ctggcagcgt caccgactcc agtgtcaccg
    12061 ccagcgccag ctctcctggg tgaagataga acgcctcgcc ctcgtcgaga acgatctcat
    12121 cgctcatcac gcggtcaagc gcggcgctca cttcatcttt gggaccgctc agatcgataa
    12181 acgctgccgt gtgaccacgg aaggtacgaa atttattgcc caggcgtaca tccaccgtcg
    12241 cgccgttaat acgctccact ggcggacgtg ggttgatcga caaacggcct tcatcaagcc
    12301 aggcttcaat atctcggtca cacagacgca tggcattttc tcctttcgca catcactccc
    12361 ttaacgccaa ttacgtcaag ggcataccaa cttatcactg aacggtacac aatttatcaa
    12421 gcttattcaa agaactgact tattttcgct ttcaatatat cgatcgcgat gcggtttttc
    12481 ccgccgcgcg gcacgataat gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac
    12541 atcgggcgca cggttttttg atattgcgcc atcacggaat ccattgaacg cccacgctcg
    12601 ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca gcggggtatc aacgaaaatg
    12661 gagaagttaa gttcgtcacg caaacgcgca tccgtcagca acaaaatgcc ttcgagaatg
    12721 atgaccttct tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc aacatagctg
    12781 taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt gtaaatgctc aagcaacagg
    12841 ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa cgcgttcttc catcgacaga
    12901 tggctttgat ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc atcaccgact
    12961 tgctcacgta attcacgata aagggtactg gcaataagac tcttgccgga agccgatgcg
    13021 ccagcgatac cgataatgac gcactgatga gactgatcag tcatatattt agcgacctga
    13081 ttaacctgga tgttaggaag ggggcgacga agcgccaaac gcggcaatta tagggatttc
    13141 atccgtctga taccagtcga atagcgttgc cgcgcgctca gagttaattg ttgacaaaga
    13201 attcccggtg gcaaattacg ttgatcagtt ttatgtaagg taaaaaatgt tatacgcagt
    13261 tgcgcaaatt atccgccttt acgtcacttt atgagcaatt cgcatataaa atgtaaaact
    13321 tttgtactag cataaacaca gaaacgaata ctggcgacct ggccttgcgg ataaagcggt
    13381 aataagcaaa caatcacagc atgtattaat tgccctgccc cacccgctgc ttcacctggt
    13441 cagtttaggt ttagtctcgt ttatctttac ccttgtctcg cttgagcttt cgcagtttgg
    13501 cacccaactc gccccactgt ggttcccgac gtccatcatg atggtggcgt tttatcgcca
    13561 tgccgggcgc atgtggccgg gaattgcgct gagctgttcg ctgggaaata tcgccgcatc
    13621 cattccagga aggccagcag gtcaaagcag gcgatttact ggcagaaatt gaccccagcc
    13681 agttcaaagt tgcattagca caaacccagg gccaactggc aaaagataaa gccacgcttg
    13741 ctaacgcccg ccgtgatctg gcgcgttatc agcaactggc aaaaaccaat ctcgtctccc
    13801 gtcaggagct ggatgcccaa caggcgctgg tcagtgaaac cgaaggcacc attaaggctg
    13861 atgaagcaag cgtcgccagc gcgcagttgc aactcgactg gagccgcatc accgcaccag
    13921 tcgatggtcg cgttggtctc aagcaagttg atgttggtaa ccaaatctcc agtggtgata
    13981 ccaccggaat tgtggtgatc acccagacgc atcctatcga tttagtcttt accctgccgg
    14041 aaagcgatat cgctactgta gtgcaggcgc aaaaagccgg aaaaccgctg atggtagaag
    14101 cctgggatcg caccaattcg aagaaattaa gtgaaggcac gctgttaagc ctcgataacc
    14161 aaatcgatgc cacaaccggt acgattaaag tgaaagcacg ctttaataat caggatgatg
    14221 cgctgtttcc caatcagttt gttaacgcgc gcatgttagt cgacaccgaa caaaacgccg
    14281 tagtgatccc aacagccgcc ctgcaaatgg gcaatgaagg ccattttgtc tgggtgctga
    14341 atagcgaaaa caaggtcagc aaacatctgg tgacgccggg cattcaggac agtcagaaag
    14401 tggtgatccg tgcaggtatt tctgcgggcg atcgcgtggt gacagacggc attgatcgcc
    14461 tgaccgaagg ggcgaaagtg gaagtggtgg aagcccagag cgccaccact ccggaagaga
    14521 aagccaccag ccgcgaatac gcgaaaaaag gagcacgctc ctgatgcagg tgttaccccc
    14581 gagcagcaca ggcggcccgt cgcgcctgtt tattatgcgt cctgtcgcca ccacgctgct
    14641 gatggtggcg atcttactcg ccgggattat cggttatcgc gccctgcccg tttcggcgct
    14701 gccggaagtg gactatccga ccattcaggt ggtcacgctc tacccaggtg ccagcccgga
    14761 tgtcatgacc tctgccgtta ccgcgccgct cgaacgccag ttcgggcaga tgtctggcct
    14821 gaaacagatg tcgtcgcaaa gttccggcgg tgcgtcagtt atcactttgc agttccagct
    14881 aacattaccg ctcaatgtcg ccgagcagga agtgcaggcc gcgattaacg ctgcgaccaa
    14941 cttgttgccg agcgatctgc ctaacccgcc ggtttacagc aaagtgaacc cggcagatcc
    15001 gccgatcatg acgctcgccg tcacctcaac cgccatgccg atgacgcaag tggaagatat
    15061 ggtggaaacc cgcgtcgcgc agaaaatctc gcagatttcc ggcgtcggcc tggtgacgct
    15121 ttccggcggt cagcgtccgg ctgttcgcgt caaacttaac gctcaggcga ttgccgccct
    15181 cggcctgacc agcgaaaccg tgcgcaccgc cattaccggc gctaacgtta actcggcaaa
    15241 aggtagcctc gacggccctt cccgtgcggt cacgctttcc gcgaacgacc agatgcaatc
    15301 cgccgaagag tatcgccagc taatcatcgc ctaccagaac ggcgcgccaa ttcgtctggg
    15361 cgatgtcgca actgtagagc aaggtgcaga aaacagctgg ctcggcgcgt gggcgaacaa
    15421 agaacaggcc attgtgatga atgttcagcg ccagcccggt gctaacatta tctccaccgc
    15481 cgacagcatt cggcagatgc tgccacagct cactgagagt ctgccgaaat cggtgaaggt
    15541 gacagtgctt tccgatcgca ccaccaatat ccgcgcatcc gtcgatgata ctcagtttga
    15601 attgatgatg gctatcgcgc tggtagtcat gattatctac ctgtttttgc gcaatattcc
    15661 ggcgaccatc attcccggtg ttgctgtacc gctgtcgtta atcggcactt tcgcggttat
    15721 ggtgtttctc gatttttcaa tcaataacct gacactgatg gctttaacta tcgccaccgg
    15781 attcgtggtc gatgacgcca tcgtggtgat cgaaaacatt tcccgctata tcgaaaaagg
    15841 cgaaaaaccg ttggcggcgg cgctcaaggg cgcaggtgaa atcggcttta ccattatctc
    15901 gctgaccttc tcactgattg cggtgttgat cccactgctg tttatgggcg atatcgtcgg
    15961 gcgactgttc cgcgaatttg ctattaccct ggcggtagcg attttgatct cagcggtggt
    16021 gtcgctgacc ctgacaccga tgatgtgcgc gcggatgctc agccaggagt cgttgcgtaa
    16081 acagaacagc ttctcccgtg cctcggaaaa aatgttcgac aggataatcg ccgcctatgg
    16141 tcgtggactg gcgaaagtgc tgaatcatcc gtggctgacc ttaagcgtgg cactcagcac
    16201 gctgctgctt agcgtgctgc tgtgggtgtt cattccgaaa ggtttcttcc cggtacagga
    16261 caacggcatt attcagggca ctttgcaggc accgcaatcc agctcctttg ccaatatggc
    16321 ccagcgacaa cgccaggtcg cggacgtgat tttgcaggat ccggcagtgc aaagcctgac
    16381 ctcatttgtt ggcgttgatg gcactaaccc gtcgctgaat agtgcacgtt tacaaatcaa
    16441 cctcaaaccg ttggatgaac gtgatgatcg ggtgcaaaaa gtcatcgccc gtctgcaaac
    16501 ggcggtagat aaagtgccgg gcgtcgatct cttcctgcaa ccaacgcagg acctgaccat
    16561 cgatactcag gtcagccgca cccagtacca gtttaccttg caggccacgt cactggatgc
    16621 gctcagtacc tgggtgccac agttgatgga aaaactccag caactgccac agctttctga
    16681 tgtctccagc gactggcagg acaaagggct ggtggcgtat gtcaatgttg atcgcgacag
    16741 cgccagccgt ctggggatca gcatggcgga cgtcgataac gccctgtaca acgcgtttgg
    16801 tcagcgactg atttccacta tttatactca ggctaaccag tatcgcgtgg tgctggagca
    16861 caataccgaa aataccccag gcctcgcggc gctggatacc attcgcctga ccagcagcga
    16921 cggtggcgtg gtgccgttaa gctcaattgc caaaattgag cagcgttttg cgccgctttc
    16981 catcaaccat ctggatcagt tcccggtaac aaccatctcc tttaacgtgc cggataacta
    17041 ttcgctgggc gatgcggtgc aggcgattat ggacaccgaa aagacgctga atctgccggt
    17101 ggatatcacc acgcaattct agggcagcac cctcgccttc cagtcggcgc tgggcagcac
    17161 tgtctggctg attgtcgcgg cggtggtggc gatgtatatc gtgctcggca ttctgtacga
    17221 gagctttatt cacccgatca ccattctctc gacgctaccc accgcagggg tcggcgcgct
    17281 gctggcatta atgattgccg gtagcgaact ggatgtgatt gcgattatcg gcattatttt
    17341 gctgatcggt attgtgaaga agaacgccat catgatgatc gacttcgcgc tggctgctga
    17401 gcgcgagcaa ggcatgtcgc cgcgcgatgc aatctaccag gcttgtctgt tgcgttttcg
    17461 tccgatcctg atgaccaccc tggcggctct gcttggcgcg ctgccgctga tgttgagtac
    17521 cggggtcggc gcggaactgc gtcgtccgtt aggtatcggc atggtcggcg gtctgattgt
    17581 cagccaggtg ctgacgctgt tcaccacgcc ggtgatttat ctgctgttcg accgcctggc
    17641 attgtggacc aaaagtcgct ttgctcgcca tgaagaggag gcgtaagtga agttttttgc
    17701 cctattcatt taccgcccgg tggcgacgat tttactgtcg gtcgccatta ccctgtgcgg
    17761 catactgggt ttccgtatgc tgccggtcgc cccgctgccg caggtcgatt ttccggtgat
    17821 tatggtcagc gcctcgctgc ccggtgcgtc gccggagaca atggcgtctt ccgttgccac
    17881 gccgctggag cgctcacttg ggcgcattgc cggggttagt gaaatgacct ccagcagttc
    17941 gctcggcagc acgcgcatta ttttgcagtt tgattttgac cgggatatca acggcgcagc
    18001 gcgtgatgtg caggcggcga tcaacgctgc acaaagtttg ctgcccagcg ggatgcccag
    18061 ccgcccgacc tatcgcaaag cgaacccgtc ggatgcgcca attatgatcc tcacgctgac
    18121 atccgatact tattcgcagg gtgaactgta cgatttcgcc tcgacgcagc tggctccgac
    18181 gatttcgcaa atcgacggtg tcggtgatgt cgatgtcggt ggcagctcgc tgcccgccgt
    18241 gcgcgtcggg ctgaccccgc aggcgctgtt taatcagggc gtgtcgctgg acgacgtacg
    18301 cactgccatc agcaatgcca acgtgcgtaa accgcagggg gcgctggaag atggcactca
    18361 ccgctggcag atccagacca acgatgaact gaaaaccgcc gctgaatatc agccgctgat
    18421 tattcactac aacaacggcg gcgcggttcg tctgggcgat gtggcgacgg tgactgactc
    18481 agtgcaggat gtgcgcaacg ccgggatgac caacgccaaa ccggctattt tactgatgat
    18541 ccgcaaactg ccggaagcca atattatcca gacggtagac agcatccggg caaaattacc
    18601 agagttgcag gagaccattc cggcggcgat tgatctgcaa attgcccagg atcgctcccc
    18661 caccattcgc gcctcgctgg aagaagtcga gcaaacgctg attatctcgg tggcgctggt
    18721 gattctggtg gtctttttat tcctgcgctc gggtcgcgcc actattattc ccgccgttgc
    18781 ggtgccggtt tcgctgattg gtacgtttgc ggcgatgtac ctgtgcggat tcagtctcaa
    18841 taatctttcg ctgatggcgc tcaccattgc tactggtttc gtggtggatg acgccattgt
    18901 ggtgctggaa aacattgccc gtcatctgga agcggggatg aaaccactac aggccgcgct
    18961 gcaaggtact cgcgaagtcg gctttacggt gctgtcgatg agtctgtcac tggtggcggt
    19021 gttcctgccg ctgctgttga tgggcggatt gccgggccga ctgttacgcg agtttgccgt
    19081 gacgctttct gtcgccattg gtatttcgtt gctggtttcc ctgacgctaa cgccaatgat
    19141 gtgcggctgg atgctaaaag ccagcaagcc gcgtgagcaa aagcgactgc gtggttttgg
    19201 ccgcatgttg gtagccctgc aacaaggcta cggcaagtcg ctgaaatggg tgctcaatca
    19261 tacccgtctg gtgggcgtgg tgctgcttgg caccattgcg ctgaatatct cgatcccgaa
    19321 aaccttcttc ccggagcagg acaccggcgt actgatgggt gggatacagg cggatcagag
    19381 tatttcgttt caggcgatgc gcggcaaatt gcaggatttc atgaaaatta tccgtgacga
    19441 tccggcagtg gataatgtca ccggctttac cggcggttca cgagttaaca gtgggatgat
    19501 gtttatcacc ctcaagccac gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg
    19561 tctgcgcgtg aagctggcga aagaaccggg ggcgaatctg ttcctgatgg cggtacaaga
    19621 tattcgcgtt ggtgggcgtc agtcgaacgc cagctaccag tacacgttgt tatccgacga
    19681 cctggcggca ctgcgcgaat gggagccgaa aatccgcaaa aaactggcga cgttgccgga
    19741 actggcggac gtgaactccg atcagcagga taacggcgcg gagatgaatc tggtttacga
    19801 ccgcgacacc atggcacggc tgggaatcga cgtacaggcc gccaacagtc tgttaaataa
    19861 cgccttcggt cagcggcaaa tctcgaccat ttaccagccg atgaaccagt ataaagtggt
    19921 gatggaagtg gatccgcgct atacccagga catcagtgcg ctggaaaaaa tgttcgttat
    19981 caataacgaa ggcaaagcga tcccgctgtc gtatttcgct aaatggcaac cggcgaatgc
    20041 cccactatcg gtgaatcatc agggattatc ggcggcctcg accatctcgt tcaacctgcc
    20101 gaccggaaaa tcgctctcgg acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg
    20161 tgtgccttcg acggtgcgcg gcagttttgc cggcacggcg caggttttcc aggagacgat
    20221 gaactcgcag gtgatcctga ttatcgccgc catcgccacg gtgtatatcg tgctgggtat
    20281 cctttacgag agttacgtac atccgctgac gattctctcc accctgccct cggcgggcgt
    20341 tggagcgctg ttggcgctgg agctgttcaa tgccccgttc agcctaatcg ccctgatagg
    20401 gatcatgcta ttaatcggca tcgtgaagaa aaacgccatt atgatggtcg attttgcgct
    20461 tgaagcccaa cggcacggta acctgacgcc gcaggaagct attttccagg cctgtctgct
    20521 gcgttttcgc ccgattatga tgaccaccct ggcggcgctg tttggcgcgc tgccgctggt
    20581 attgtcgggc ggtgacggct cggagctgcg gcaacccctg gagatcacca ttgtcggcgg
    20641 tctggtgatg agccagctcc tgacgctgta taccacgccg gtggtgtatc tctttttcga
    20701 ccgtctgcgg ctgcgttttt cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga
    20761 tcttcccgac agcacccgct ggcaattatg gattgtggct ttcggcttct ttatgcagtc
    20821 gctggacacc accatcgtaa acaccgccct tccctcaatg gcgcaaagcc tcggggaaag
    20881 tccgttgcat atgcacatgg tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc
    20941 cgccagcggc tggctggcgg acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt
    21001 gctgtttact ctcggttcac tgttttgcgc gctttccggc acgctgaacg aactgttgct
    21061 ggcacgcgcg ttacagggcg ttggcggtgc gatgatggtg ccggtcggca ggttgacggt
    21121 gatgaaaatc gtaccgcgcg agcaatatat ggcggcgatg acctttgtca cgttacccgg
    21181 tcaggtcggt ccgctgctcg gtccggcgct cggcggtctg ctggtagaat acgcgtcgtg
    21241 gcactggatc tttttgatca acattccggt ggggattatc ggtgcgatcg ccacattgat
    21301 gttaatgccg aactacacca tgcagacgcg gcgctttgat ctttccggct ttttattgct
    21361 ggcggttggc atggcggtat taaccctggc gctggacggc agtaaaggta caggtttatc
    21421 gccgctggcg attgctggcc tggtcgcagt tggcgtggtg gcactggtgc tttatctgct
    21481 gcacgccaga aataacaacc gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt
    21541 ttcgctgggc ctggcgggga gctttgccgg acgtattggc agtggcatgt tgccctttat
    21601 gacaccggtt ttcctgcaaa ttggcctcgg tttctcgccg tttcatgccg gactgatgat
    21661 gatcccgatg gtgcttggca gcatgggaat gaagcgaatt gtggtacagg tggtgaatcg
    21721 ctttggttat cgtcgggtac tggtagcgac cacgctgggt ctgtcgctgg tcaccctgtt
    21781 gtttatgact accgctctgt tgggctggta ctacgttttg ccgttcgtcc tgtttttaca
    21841 agggatggtc aactcgaccc gtttctcctc catgaacacc ctgacgctga aagatctccc
    21901 ggacaatctg gcgagcagcg gaaacagcct gctgtcgatg attatgcaat tgtcgatgag
    21961 tatcggcgtc accatcgccg ggctgttgct ggggcttttt ggttcgcagc atatcagcgt
    22021 cgacagcggc accacacaaa ccgtctttat gtacacctgg cttagcatgg cgtttatcat
    22081 cgcccttccg gcgttcatct ttgccagagt gccgaacgat acgcatcaaa atgtagctat
    22141 ttcgcggcga aaaaggagcg cgcaatgaag ttctggcgac ccggtattac cggcaaactg
    22201 tttctggcga ttttcgccac ctgcattgtc ttgctgatca gtatgcactg ggcggtgcgt
    22261 atcagttttg agcgtggctt tattgattac atcaaacacg gcaatgaaca gcgattacag
    22321 ctgttaagtg atgcgcttgg cgagcagtat gcgcagcatg gtaactggcg cttcctgcgc
    22381 aacaacgatc gctttgtctt tcagatcctg cgttcatttg aacacgataa ttcggaagat
    22441 aaacccggcc cgggtatgcc gccgcacggc tggcgtaccc agttctgggt ggtcgatcaa
    22501 aacaacaaag tactggttgg tccgcgagcg ccgattccac ctgacggtac acggcgaccc
    22561 attctggtca acggtgcgga agtgggcgcg gtgattgcct cccctgttga gcggctgacc
    22621 cgcaatactg atatcaattt cgataaacaa cagcggcaaa ccagctggct gatcgtcgcc
    22681 ctggcaacgt tactcgcggc gctcgccacc tttctgctgg cgcgcggttt gctggcaccg
    22741 gtaaaacgac ttgtcgatgg cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta
    22801 acgcccacca gtgaagatga actgggcaaa ctggcgcaag acttcaacca gctcgccagc
    22861 acgctggaga aaaaccagca aatgcggcgc gattttatgg ccgatatttc tcacgaactg
    22921 cgtacgccat tagcggtgct gcgcggtgaa ctggaagcca ttcaggatgg cgtgcgcaaa
    22981 ttcacgccgg agacggtggc gtctttacag gcggaggtcg gtacactgac caaactggtt
    23041 gacgatcttc atcagttgtc gatgtctgat gaaggcgctc tcgcctatca aaaagcaccg
    23101 gtagatttga tcccactgct ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagc
    23161 cgtggcctga aactgcaatt ttccctgcca gacagtatca ccgtatttgg cgatcgcgac
    23221 cgtttaatgc agttattcaa taacttactg gaaaacagcc tgcgctacac tgacagcggc
    23281 ggcagcctga aaatctctgc cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac
    23341 agtgcgccag gtgtcagtga cgatcagcta caaaaattgt ttgaacgttt ttatcgcacc
    23401 gaaggttccc gcaaccgcgc cagcggcggt tccgggctgg ggctggcgat ttgcctgaac
    23461 attgttgaag cccataatgg ccgcattatt gccgcccatt cgccttttgg cggggtaagc
    23521 attacagtag agttaccgct ggaacgggat ttacagagag aagtatgacc gagttaccaa
    23581 tcgacgaaaa cacaccgcgt attttgatcg tggaagatga accgaagctg gggcagttgc
    23641 tcattgatta tctgcgtgct gcgagctatg cgccgacgct tatcagccac ggcgatcagg
    23701 tactggcgta tgtgcgccag acaccaccgg atctgatcct gttggatctg atgctccctg
    23761 gcaccgatgg cctgacatta tgccgggaaa ttcgtcgttt ttccgatgtt ccgatcgtga
    23821 tggtgacggc aaaaatcgaa gagatcgacc gcctgctggg gctggagatt ggcgccgatg
    23881 actacatctg caagccgtac agcccacggg aagtggtagc gcgtgtcaaa accattttgc
    23941 gccgttgtaa accgcaacgc gaacttcagc aacaggatgc cgaaagcccg ttgattatcg
    24001 acgaaggtcg ttttcaggct tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat
    24061 ttcgtctgct taaaacgctc tctcacgaac caggaaaagt gttctcccgc gagcaattgc
    24121 tcaatcatct ttatgacgac taccgcgtag taaccgaccg caccattgac agccacatta
    24181 aaaatctgcg ccgcaagctg gaatctctcg acgccgaaca gtcatttatc cgcgccgttt
    24241 acggcgttgg ttaccgctgg gaagccgacg cctgccgcat cgtttagttt tagcgacatt
    24301 actttgttag ccggagatgg cgttcagacc cgatccggca acattattcc acgcatggtc
    24361 agcaaactga cattatctct ccgccctggc aggcatcaga aaatcgctca tactttaatc
    24421 agtaaacagc acctttagat gctgttttcg ataagcaatt tcaatcaagg agtcattatg
    24481 gctggttggt ttgaactcag taagagcagt gataatcagt tccggtttgt gctaaaagcg
    24541 ggcaatggtg agactatcct caccagcgag ctgtatacct caaaagcctc tgcggaaaag
    24601 ggcatcgcgt cggtgcgtag caacagcccg caagaagagc gctatgagaa aaaaacggca
    24661 agcaacggca aattctattt caatctgaaa gccgctaatc atcaaattat cggctccagc
    24721 cagatgtacg ccaccgcgca atctcgtgaa accggaattg cctccgttaa agccaatggc
    24781 acaagccaga cggtgaaaga caatacgtaa ttacgatgcc gggcgtgggt tgttggcgtc
    24841 cggcgcttta ccagatcccc cctacagcgc tacaatgccc gcccttaaag tgggggcctc
    24901 ccctaaccgc ttcatcaggt gaagcggatc tgacctgtca tcagaacgag agaattatgt
    24961 ttaaaccgga actcctttcc ccggcgggaa cgctgaaaaa tatgcgttac gctttcgctt
    25021 atggcgcaga tgctgtttat gcgggccagc cgcgttactc cctacgtgtg cgcaacaacg
    25081 aattcaacca cgaaaatctt cagctcggca tcaatgaagc ccacgcgctg gggaaaaagt
    25141 tttatgtcgt ggtcaacatt gcaccgcaca acgccaagct gaaaaccttt atccgtgacc
    25201 tgaaaccggt ggtggaaatg gggccggatg cgctgattat gtccgatcca gggctgatta
    25261 tgctggtgcg tgagcacttc cctgaaatgc cgatccacct ctcggtgcag gctaacgccg
    25321 tgaactgggc gacggtgaaa ttctggcagc aaatgggact gacccgcgtg atcctctctc
    25381 gcgagctgtc gctggaagag attgaagaga tccataatca ggtgccggat atggagatcg
    25441 agatcttcgt tcacggcgca ctgtgcatgg cctactccgg tcgctgcctg ctctctggct
    25501 atatcaacaa gcgcgacccg aaccagggca cctgcaccaa cgcctgccgc tgggagtaca
    25561 acgtccagga agggaaagaa gatgacgttg gcaacatcgt acacaagtac gagccgattc
    25621 cggtgcaaaa tgttgagccg acgctgggta tcggcgcgcc aaccgacaaa gtgtttatga
    25681 tcgaagaagc ccagcgtccg ggcgagtata tgaccgcgtt tgaagatgag cacggcactt
    25741 acatcatgaa ctcgaaagat ctgcgcgcca tcgcccatgt agaacgcctg accaaaatgg
    25801 gcgtgcattc gctgaaaatc gaaggtcgta ccaaatcttt ctactattgc gcacgcaccg
    25861 cacaggttta ccgcaaagct atcgatgacg ccgctgcggg aaaaccgttc gataccagcc
    25921 tgctggaaac tctggaaggt ctggcgcatc gtggctatac cgaaggtttc ctgcgtcgtc
    25981 atactcacga cgattatcag aactacgaat acggttattc agtttctgac cgccagcagt
    26041 ttgttggtga gtttaccggt gagcgcaagg gggacctcgc agcggtagcg gtgaaaaata
    26101 aattctccgt tggcgacagt cttgagctga tgacgccgca aggcaacatt aactttaccc
    26161 ttgagcacat ggaaaacgcc aaaggcgaag ctatgccggt cgcaccaggc gatggttata
    26221 ctgtgtggct cccggtcccg caggatcttg agctaaatta cgcgctgcta atgcgtaatt
    26281 tctccgggga aaccacgcgt aacccccacg gtaagtgatt aatttcgatt atttttcccg
    26341 gatggaaaat tcttagaaac cgatcacata cagctgcatt tattaaggtt atcatccgtt
    26401 tcgctgaaaa acataatcca taaaatgcta gctgtaccag gaaccacctc cttagcctgt
    26461 gtaatctccc ttacacgggc ttattttttg tacataagct gtaaagaagg attgatccct
    26521 gataaccgtc ttccattgac cacatcgata gaatccccct tcatagcaca atgcctttca
    26581 cgtaacggta tcgtgctcgc ccaggttccg gctacgcaca gccagaacgc gcatgtttga
    26641 cgcttacaaa aaaatattct cactctccac atttgaatgt cagacgagcg acgccatgta
    26701 atcctgcacc ttctgtcttc aggtcaacta tctgcatttt tttgccctga gtaacacaga
    26761 aatgggctgc atcatttttt actatatttt ctgcaccaga tattctgccc ctggctaaag
    26821 aagcttcggc ttcggtgtag tattggttat cgagtttacg ctgaatatta cttttatatg
    26881 caagaccaaa tttaccgata cttgtttcat cattatgcac agcacaacca gatataagaa
    26941 aaatactaat taatgatata gcagctatct ttttcatctc accttccccc attaaatacc
    27001 aacgacactc tctagtgttt aaatataata atggcatgat tattataatt gaataggatt
    27061 ataataaatg ttctgtacaa catttcctac ataagtagga attacggaca ttgaggccct
    27121 tcagggtaac tccatggggg ctttaatata ttatattgaa gatgccactg tttagttgaa
    27181 tattaggtat atgctctttt ttgaaattta tcggtggcag ccgttagtat tcgctgtccc
    27241 cattgcaagc tcctggtggt aaccactgaa tcctccatac ttgaactgac tttttatcct
    27301 ccgactttca tcctgttctg actccacctt ttgttttctg ctctacacta tctacagacc
    27361 aatcataaag gcacatacga tcatggcaga atttcccgcc agcttactga ttcttaatgg
    27421 taaaagtact gacaatctac ccttgcgcga agcaattatg ctgttgcgtg aggaaggaat
    27481 gacgatccat gtgcgggtca cctgggagaa aggtgatgcc gcacgatttg tagaggaggc
    27541 ccggaagttg ggcgtcgcaa cggtgattgc cggtggtggc gatggcacca ttaatgaagt
    27601 ttctacggcg ttgattcagt gtgaggggga tgacataccc gcgctgggaa ttttgccatt
    27661 aggaaccgcc aatgattttg ccaccagtgt agggattcct gaggcactgg ataaggcgct
    27721 gaaactggca attgccggta atgccattgc gatagatatg gcgcaggtca acaaacaaac
    27781 ctgttttatt aatatggcga caggcggatt tgggacgcgt attaccacag aaacgccgga
    27841 aaaattaaaa gccgcgctgg gtggcgtctc ttacatcatt catggcttaa tgcgcatgga
    27901 tactctgcaa ccggaccgtt gtgaaatccg cggtgaaaac tttcactggc aaggtgacgc
    27961 cctggtcatt ggtattggta acgggcgtca ggccggtggc ggtcaacaat tgtgcccgaa
    28021 cgcgttaatt aacgatggct tgctgcaact gcgcattttt accggcgatg aaattcttcc
    28081 ggctctcgta tcaaccttaa aatctgacga agataacccg aatattatcg aaggcgcttc
    28141 gtcgtggttt gatatacaag ccccacacga aatcactttt aatcttgatg gcgaaccgtt
    28201 gagtgggcaa aacttccata ttgaaatact tccggcggcg ttgcgttgtc gattaccacc
    28261 agattgtcca ttattgcgtt aatcaaacta ttctctcttg aaccagggag aatccgtaat
    28321 gccggatgcg gtgtaaacac cttatccggc atacagaaca atactacacc gtaattaatt
    28381 ttacccgtgt cttctgcaat gccaatttat ccacctctga cacagcatca tcggtaacaa
    28441 taatgtcaat tttttcgatt ggcagtacct gattaaaacc gcgacgatta aatttcgatg
    28501 aatcgagaac ggcaatcact ttatttgcgg cggtgaccat cgccccgcta atcgaataac
    28561 cttcattaaa ggtcgtaata ccattaaccg catcaatacc atcagcaccg acaaacatta
    28621 aatcggcatt aatatcctgc aatgaacgct cggcaatgga accatgcatc gagcgcgttt
    28681 tatggcggac cgtgccacca cagacgacca gagtaatgtc tttattttcg gaaagggcaa
    28741 acgctgccgg gagactgttg gtaatcaccg tgatattttt agcggtcatt aatccttcgg
    28801 cgataagcat tgtggtactt ccgctgtcga gaataacggt catcccttca tggatcattt
    28861 tgactgccgc ctgcgctatt ctgttttttg gcgcgctggc gagctgaaat cgctctttaa
    28921 acccgacttc ctgggtctcg gtttcactat taccggacat tattttcgcc gcaccgccat
    28981 gaaagcgcgt aaccacgcct ttttgttcga gaaagcgcaa atcggcacgg attgtcgctt
    29041 ccgaggcagc aaatactccc gccagatcct gaacaagcac ggttccctgt tcattcacta
    29101 attggatgat cttattcctt cgctcgaatg aattcatcgt catttaccca tattcaattg
    29161 tggctagtgt aaacgaaggg ggaccattgg tgaatgatct gttttgcaca ataccgggcc
    29221 agcatcacgc tggcccgcgg tttcatggaa tgagcaacac tttgccctgc atggcattac
    29281 gagcgatgtc acgcaccgcc tgggtgaagc tttcaaagct tccacggtga gcgattaatg
    29341 gctccaggct taacttacgt tctgtcagca accggctcgc cgtttcccac tcctgccccg
    29401 gccaagggct ggaatagttc atccaactgc cgataaccgt cagctcttta cgcaatattt
    29461 tgccaaacgt tgccgatgtt aaatgcagat cctgatgcaa cgtgcccacc agcgccagtt
    29521 gggcatgagg accggcaatc tctaccgcca gttcgacagt ttgcggtacg ccagccgtct
    29581 cgaggataag ctgattaaag cgcagttcgc gtaaaacgct ctgcatttgc ggcgcgctca
    29641 tttcgctact gttaaatgtt tgcatcgcac cgaaagattt tgccagtgcc agtttttctg
    29701 agctaatgtc gatcgccgtc acactctttg ctcccagcgc gacagcgcac tgaatagcca
    29761 gcaggccaat ggttccggca ccaataataa taacgttttt attctcacaa ccttgcgcta
    29821 aatgaaaagc atgcaggcca acggtaatcg gctcaataaa agccccatcc tcaataggca
    29881 tatccgtggg tagagcaaag acattttttc gcttaacgac aatatattca gcaaatccac
    29941 catcacgccg tgagccaata aaatcatatt ttgcgcactg ggaataaaac cctttcaaac
    30001 actctggaca agtaaaacag ggtaataacg gcacacaggc aaccgcatcg ccagggtgta
    30061 aatcatcaac accggatccc accgcatcaa tatagccgct aaattcatgg cctaacgtta
    30121 ttggataata atgtgcacca tttttaaata tcctgggtaa atcggaacca cataagcccg
    30181 agctggcaat ttttacccgc acctcatcct gatgtttaat ttcaggaatg acgctttttg
    30241 caacgcgcac gataccatca gtatcattca ctactgattt cataaaaact cctgatt
//
LOCUS       DAETQL010000046        29946 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.046, whole genome shotgun sequence.
ACCESSION   DAETQL010000046 DAETQL010000000
VERSION     DAETQL010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.046"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            56..850
                     /locus_tag="K0W65_002252"
     CDS             56..850
                     /locus_tag="K0W65_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBI3095502.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            872..2281
                     /locus_tag="K0W65_002253"
     CDS             872..2281
                     /locus_tag="K0W65_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3095503.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2259..3038
                     /locus_tag="K0W65_002254"
     CDS             2259..3038
                     /locus_tag="K0W65_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBI3095504.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3035..3895
                     /locus_tag="K0W65_002255"
     CDS             3035..3895
                     /locus_tag="K0W65_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBI3095505.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4043..4618)
                     /locus_tag="K0W65_002256"
                     /pseudo
     CDS             complement(4043..4618)
                     /locus_tag="K0W65_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4635..4895)
                     /locus_tag="K0W65_002257"
     CDS             complement(4635..4895)
                     /locus_tag="K0W65_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBI3095506.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4886..6157)
                     /locus_tag="K0W65_002258"
     CDS             complement(4886..6157)
                     /locus_tag="K0W65_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI3095507.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6235..6597)
                     /gene="queD"
                     /locus_tag="K0W65_002259"
     CDS             complement(6235..6597)
                     /gene="queD"
                     /locus_tag="K0W65_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBI3095508.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6916..8715
                     /gene="cysJ"
                     /locus_tag="K0W65_002260"
     CDS             6916..8715
                     /gene="cysJ"
                     /locus_tag="K0W65_002260"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBI3095509.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8715..10427
                     /gene="cysI"
                     /locus_tag="K0W65_002261"
     CDS             8715..10427
                     /gene="cysI"
                     /locus_tag="K0W65_002261"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBI3095510.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10501..11235
                     /gene="cysH"
                     /locus_tag="K0W65_002262"
     CDS             10501..11235
                     /gene="cysH"
                     /locus_tag="K0W65_002262"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBI3095511.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11499..11651
                     /locus_tag="K0W65_002263"
     CDS             11499..11651
                     /locus_tag="K0W65_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBI3095512.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11845..12936
                     /locus_tag="K0W65_002264"
                     /pseudo
     CDS             11845..12936
                     /locus_tag="K0W65_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13082..14119)
                     /gene="iap"
                     /locus_tag="K0W65_002265"
     CDS             complement(13082..14119)
                     /gene="iap"
                     /locus_tag="K0W65_002265"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBI3095513.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14371..15279
                     /gene="cysD"
                     /locus_tag="K0W65_002266"
     CDS             14371..15279
                     /gene="cysD"
                     /locus_tag="K0W65_002266"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBI3095514.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15281..16708
                     /gene="cysN"
                     /locus_tag="K0W65_002267"
     CDS             15281..16708
                     /gene="cysN"
                     /locus_tag="K0W65_002267"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBI3095515.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16708..17313
                     /gene="cysC"
                     /locus_tag="K0W65_002268"
     CDS             16708..17313
                     /gene="cysC"
                     /locus_tag="K0W65_002268"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBI3095516.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17363..17686
                     /locus_tag="K0W65_002269"
     CDS             17363..17686
                     /locus_tag="K0W65_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBI3095517.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17880..18191
                     /gene="ftsB"
                     /locus_tag="K0W65_002270"
     CDS             17880..18191
                     /gene="ftsB"
                     /locus_tag="K0W65_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBI3095518.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18210..18920
                     /gene="ispD"
                     /locus_tag="K0W65_002271"
     CDS             18210..18920
                     /gene="ispD"
                     /locus_tag="K0W65_002271"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBI3095519.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18920..19399
                     /gene="ispF"
                     /locus_tag="K0W65_002272"
     CDS             18920..19399
                     /gene="ispF"
                     /locus_tag="K0W65_002272"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBI3095520.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19396..20445
                     /gene="truD"
                     /locus_tag="K0W65_002273"
     CDS             19396..20445
                     /gene="truD"
                     /locus_tag="K0W65_002273"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBI3095521.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSCEVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20426..21187
                     /gene="surE"
                     /locus_tag="K0W65_002274"
     CDS             20426..21187
                     /gene="surE"
                     /locus_tag="K0W65_002274"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBI3095522.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21181..21807
                     /gene="pcm"
                     /locus_tag="K0W65_002275"
     CDS             21181..21807
                     /gene="pcm"
                     /locus_tag="K0W65_002275"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBI3095523.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21947..23086
                     /gene="nlpD"
                     /locus_tag="K0W65_002276"
     CDS             21947..23086
                     /gene="nlpD"
                     /locus_tag="K0W65_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBI3095524.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23149..24141
                     /gene="rpoS"
                     /locus_tag="K0W65_002277"
     CDS             23149..24141
                     /gene="rpoS"
                     /locus_tag="K0W65_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBI3095525.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24261..24668)
                     /locus_tag="K0W65_002278"
     CDS             complement(24261..24668)
                     /locus_tag="K0W65_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBI3095526.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24815..25408
                     /locus_tag="K0W65_002279"
     CDS             24815..25408
                     /locus_tag="K0W65_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HBI3095527.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25408..26355
                     /locus_tag="K0W65_002280"
                     /pseudo
     CDS             25408..26355
                     /locus_tag="K0W65_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26628..27191)
                     /locus_tag="K0W65_002281"
     CDS             complement(26628..27191)
                     /locus_tag="K0W65_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095528.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27356..27607
                     /locus_tag="K0W65_002282"
     CDS             27356..27607
                     /locus_tag="K0W65_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3095529.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27609..29705
                     /locus_tag="K0W65_002283"
     CDS             27609..29705
                     /locus_tag="K0W65_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3095530.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7327 a         7411 c         8097 g         7111 t
ORIGIN      
        1 tcgtcacatc atcatcggca acgaaggtgc attgtgctat atcactgaag taacagtgaa
       61 aatctttaaa tacaccccgg aaaacaacct cttctacggc tatatcctgg aagacatgaa
      121 aaccggcttc aacatcctgc gtgaaatcat ggtggaaggg tatcgtccgt cgatcgctcg
      181 tttgtatgac gctgaagatg gcacccaaca cttcacccat tttgccgacg gaaaatgtgt
      241 gctgatcttt atggctgaag gtaaccctcg cattgcgaag gcgacgggcg aagggattgc
      301 ggaaatcgtt gcccgctacc cgcagtgcca gcgcgtggac agcaaactga ttgaaacctg
      361 gtttaacaac ctgaactggg gaccggataa agtggctgcc gaacgtgtgc agatcctcaa
      421 aaccggcaac atgggcttta ccaccgaagt gtccggctgc tggagctgca tccacgaaat
      481 ctacgaaagc gttattaacc gtattcgtac tgagttcccg cacgccgacg acatcaccat
      541 gctgggcggt cactcctctc atagctatca gaacggcacc aacatgtact tcgtctatga
      601 ctacaacgtt gttgactgta agccggaaga ggaaatcgac aagtaccaca atccgctcaa
      661 caaaatcatc tgcgaagaaa ccattcgcct cggcggttcg atggtgcacc accatggcat
      721 tggtaaacat cgcgttcact ggagcaagct ggaacacggc agcgcgtggg cgttgctgga
      781 agggctgaaa aagcagttcg atcctaacgg cattatgaat acgggtacta tctatccgat
      841 tgaaaaataa tgtatcaggc agcgttccgc gatgacgggg cgttgccttt tcggcttctc
      901 aggcgagaag ccgttcttat taccggacaa tgaaggggta aagatgaaca cttcaccggt
      961 gcgaatggat gatttaccgc ttaaccgttt tcactgtcga attgctacgc tcactttcgg
     1021 cgcacacctg accgacggtt atgttctagg cgtcattggt tacgccatta ttcaacttac
     1081 gcccgccatg caactgacgc cgtttatggc gggaatgatc ggcggctcgg cgctccttgg
     1141 tttgtttctt ggcagcctgg ttcttgggtg gatctccgac catattggtc ggcaaaaaat
     1201 cttcaccttc agctttttgc tgattacgct cgcttcgttc ttgcaatttt ttgccaccac
     1261 gccagagcat cttattgggc tgcgcatttt gatcggcatt ggtctgggag gcgattactc
     1321 agtaggtcac accttgctgg ctgaattttc cccgcgccgc catcgcggta ttttgctggg
     1381 cgcattcagc gtggtgtgga ccgtaggcta tgtgttggca agtattgccg gacatcactt
     1441 tatttccgaa aacccggagg cctggcgctg gttgctggca tcggcagctc tgcccgcgtt
     1501 gttgattacg ttattacgct ggggaacgcc agaatcgcca cgttggctac tgtgccaggg
     1561 gcgttttgca gaagctcacg ctatcgtgca tcgctatttt ggtccccatg ttttactggg
     1621 cgatgaagtg gtaacggcga cccataaaca catcaaaacc ttgttctctt cgcgttactg
     1681 gcggcgcacg gcgtttaaca gcgtcttctt tgtctgcctc gtaatcccat ggtttgtgat
     1741 ttatacctgg ctgccaacta tcgcccagac tattggtctg gaagatgcgc tgactgccag
     1801 cctgatgctt aatgcgttgt taattgtggg cgcgctgctg ggattagttc tgacgcacct
     1861 gctggcacat cgcaaatttt tgctgggaag ttttttgctg ctggcggcaa cgctggtagt
     1921 aatggcctgt ttgccttccg gcagttcatt aacgctgctg ctttttgttc tcttcagcac
     1981 caccatttcg gcagtcagta atctggtggg cattttgcct gcggaaagtt ttcctactga
     2041 cattcgctcg ctgggagtcg gttttgccac cgccatgagt cgacttggcg cggcggtaag
     2101 tactggcctg ctgccgtggg tgctggcgca gtggggaatg caagtcacct tattgctcct
     2161 agcgacagtg ttgttggttg gttttgttgt gacctggcta tgggcaccag aaactaaagc
     2221 cctcccgctg gtggcggcgg gaaatgtagg aggtgcgaat gaacattctg ttagcgttta
     2281 aagccgaacc ggatgccgga atgctggcgg aaaaagagtg gcaggcggcg gctcagggta
     2341 atagcggacc ggatgtttcg ctactgcgaa gtttactcgg tgctgatgaa caggccgctg
     2401 ccgcgctgct gttagcgcag agaaaaaacg gtacgccgat gtctttaacc gcgttgagca
     2461 tgggggatga acgggcgttg cactggctgc gctatctcat ggctcttggg tttgaggaag
     2521 ctgtattgct ggagacggcg gcagatctgc gctttgcgcc ggaatttgtc gctcgccata
     2581 ttgccgaatg gcagcgtcag aatccgctgg atctgataat taccggcagc caaagcagcg
     2641 aagggcagaa tgggcaaacg ccatttttgc tggcggaaat gctgggctgg ccctgcttca
     2701 cccaggtgga gcgtttcact ctcgacgcgc tgtttatcac cctcgaacag cgtactgaac
     2761 atgggttgcg ctgttgccgg gttcgcttgc ctgcggtgat tgccgtgcgt cagtgcggtg
     2821 aagtggcctt gccagtaccg ggaatgcgcc agcgaatggc tgcggggaaa gcagaaatta
     2881 tccgtaaaac tgttgctgcc gaaatgccag cgatgcagtg tctgcaactg gcaagagcag
     2941 agcaacgacg cggagcgacg ctgattgatg ggcaaacggt agcagaaaaa gctcaaaagc
     3001 tatggcagga ctatttacgc caaaggatgc agccatgaat atcgcaattg tgacaataaa
     3061 tcaggagaat gccgcaatag cgagctggct ggcggcgcag gatttcagcg gttgtacgct
     3121 ggcgcactgg caaatagaac ctcagcccgt agtggcggaa caggtgctgg atgcgatggt
     3181 ggaacagtgg cagcgaacgc ctgcggacgt tgttctcttt ccgccgggaa cgtttggcga
     3241 tgaactgtcg acccggctgg cctggcgttt acatggtgca agcatctgtc aggtgacttc
     3301 gttagatatt ccaaccgtaa gcatgcgcaa atcccactgg ggaaatgcgt taacggcgac
     3361 gctgcaaaca gaaaaacgcc cgttatgcct ttccctggcg cgtcaggcgg gagcagcgaa
     3421 aaacgccacg ttgccctctg gaatgcagca actgaacatt gtcccaggtg cgcttcccga
     3481 ctggctggtt agcacagagg atctgaaaaa tgttagccgc gacccattag ctgaggccag
     3541 acgggtgctg gtggtgggac agggcggaga ggcagataac caggaaatcg ttatgctggc
     3601 agaaaagctg ggagccgaag tgggctacag tcgggcgcga gtgatgaacg gtggcgttga
     3661 tgctgaaaaa gtgattggta tttccggaca tctgctggca cctgaggtgt gcattgttgt
     3721 tggcgcttcc ggcgcagcag cgttaatggc gggcgtacgc aatagcaagt ttatggtggc
     3781 gattaatcat gacgccagcg cagcggtatt ttcacaggca gatattggcg tggttgatga
     3841 ctggaaagtg gtgctggagg cactggtgac aaacattcac gcagaatacc aataacgtgc
     3901 tgccggatga tcctgcctac ggttctggac tggggacctg taggcctgat aagacgcgtt
     3961 aagcgtcgca tcaggcgctg aatgtcggat gtggcgtgcg gccaacaaat caaaacaaat
     4021 tcaattggtt acgattatcc cgtcaaagca attttttcgc taacgtccag actccggtat
     4081 tcgtggtgga aagcgccacg acacccgcgt taatcgcatt acgcgcatct tcttcatcgc
     4141 ataccagccc gccagcaatc agcggttggc ggattttttc cgtcacccag cccagcactt
     4201 tgggcatgca gcctggcagg atctcaatac aatccggatt cgattgcgca acttacttat
     4261 caatgttgtg aaacgaaatc gaatcaacaa taaacaggtg atgaatacag aaaaaaactt
     4321 ccgctcttgc cgcttttagc atcgatgctt tggtgctgat aattccatcc gcctcggtca
     4381 ccagtttcaa aaactgaatc accacttctt tatttgacgc gccttccagc aaatcaacat
     4441 gaataaaagc atatttcccg gcgttcttaa tctttttaac gatattactg atggtgcaga
     4501 tattgccgta caacacggaa ataaactggc attcagaatc gatggccagt tgcaggctgg
     4561 cattatcttt aacggcagca atcaccggat tctggcggag caggtgtaag aggggcataa
     4621 attatcctta ttatttatcc aaagcggtag gtgatgccga atccggaagg cggatagcgc
     4681 cactgttgta gtgtggattc gtcgcacagc aaacggcagg tgccacactc caggcagccg
     4741 cgatagtcaa tacgtaagtc accttcctgt gtgagagaaa aaagacctac cggacaggcg
     4801 ttaatcaacc gttgtaccat ctgacgctca acggagtcag ccggaacaat gtgcggcgca
     4861 tcagcaacgc gccagagatt acgggctaca gacatcgcag actcctgata acatcgcccg
     4921 ccagatgcca caggccatga cgacgtaaat gatgccagaa taactggcgc agcggtggaa
     4981 caggtttatc accctgatcc cataaatcgc gggaaatatc ctgcattaac gcaggccacg
     5041 cccgatacct gccagggcgt tgcaaaagcg ccggaacatg ctgataacgt tgtagaacat
     5101 cccatagcag gctgcgctct acgttgtgat gataaagcgc aaacagattt tgcggctcgc
     5161 ggtgctggca ggcgcttatt agcgtttgtg ccgccgcctg tgcgccagtc agcgccatat
     5221 ccatgccgcg cacggaaatt ccggtattga cgcaactgcg caacgcatcg cccaccagca
     5281 gccagccgtt accggcgtat tgcaccggca tactgtgcaa gccaccttct ggcaccagat
     5341 gcgcaccata ctccagtgat tccgtgtttt tgataagcgt gcgcactgcc ggatgcgctt
     5401 taaagcgagt cagcagctcg cttgccggaa cacgactttg cgtaagggaa gagagcgggc
     5461 aaacaatccc taacgagagc gtttgttgat tagtataaag aaatgcgccg ccgggtaagt
     5521 catcacagat tccgccgctg aacagcaacg ctgcgccttc gttattctcc agatgaaaac
     5581 gttcttcaat agcggatgtt tccagcgaca gcacttcttt gatccccaac gccatcgctt
     5641 cgccagcagg acgagtcact aacccgtgac gttcagccag gacgctgttg gcaccttctg
     5701 ccagcaccac ataacgggcg cggagaatat cgtcaccaca aataacgcca cagactctgc
     5761 cgttttcttc atacagtgca tccaccgtcg ctccggggat gcattcgaca ccttcttttt
     5821 cggcttcggc aaccagccac ggatcgaacc gtgcacgtaa tacattccag gattcaccgc
     5881 cgggctgtaa gctggaaaac gtcgttgcgc catccggcgt taacagggaa aggctttcgt
     5941 gagtgatgcg tcgttcaaga ggcgcggtca gatgaaattg cgggaggagt tccgcgagtg
     6001 catgggtata taaccgcctg ccggaaagat ttttgctgcc ggggatttca gcgcgttcga
     6061 gtaacaaaac ggataaatcc gctcgcgcgc agcgtaacgc gcaagcggtg cctgcaatac
     6121 cggcaccaat aataataatg tcgcagtcgt cttccatgtc atgccccaga tatccagaac
     6181 acaacaccct aacatagcgt tactaaaggg aaattgaccg ccgacacact cttatcattc
     6241 gccgcgatag atacaccctg cggtgcaggt ttcttttacc atcaccgcac ttaacagcgg
     6301 cacaacgggt ttaacctgat cccaaatcca ttttgctaaa acttcgctgg ttggattttc
     6361 cagacctgga atatcattga gataatggtg atcgaggcgc tcataagttg gcttaaacgc
     6421 cgctttcagt tcagcgaaat cgataatcca gcccgtatgc ggatcgactt ccccggtaat
     6481 ttccagtcgc accataaagg aatgcccgtg caggcgacca catttatgcc cttccgggac
     6541 atgtggtaag cggtgagcgg cttcgaaggt gaaatcttta aataacgtgg tggacatcat
     6601 aatttctcta caggacgata aaaccgccgt agagtaccgg aaaggctgtt ttttcgcatt
     6661 atctaaaacg actttgttgc gcaaaatcgc tgatttatct taatgattgg ctaaattcat
     6721 ttgtttttca ttaggttggt taatctattt tgttgttaaa gactattgct aaaacaggtt
     6781 agtcgatttg gttattagtt atcgctatcc cgtctttaat ccacaccgtt caccccgtta
     6841 accttacctt ctcttctgtt ttatgggcgc tgacagggcg cagaaacagc tttgcttact
     6901 ggaacataac gacgcatgac gacacaggtc ccaccttccg cgttgcttcc gttgaacccg
     6961 gagcaactgg cacgccttca ggcggccacg accgatttaa cgcctaccca gcttgcctgg
     7021 gtttctggct atttctgggg cgtgctcaac cagcagcctg ttgcgcttgc agtgacgcca
     7081 gcgcccgccg cagaaatgcc gggtataacg attatctctg cttcgcaaac cggcaatgcg
     7141 cgccgggttg ctgaagcatt acgcgatgat ttattagcag caaaactgaa cgttaagctg
     7201 gtgaacgcgg gcgactataa attcaaacaa atcgccagcg aaaaactgct catcgtagtg
     7261 acgtcaacgc aaggggaagg ggaaccgccg gaagaagccg tcgcgctgca taagttcctg
     7321 ttctccaaaa aagcgccaaa gctggaaaac accgcgtttg ccgtgtttag cctcggcgat
     7381 agctcttatg aatttttctg ccagtccggg aaagattttg acagcaagct ggcggaactg
     7441 gggggtgaac gcctgctcga ccgtgtcgat tctgatgttg aataccaggc tgctgccagc
     7501 gagtggcgcg cccgcgtggt tgatgcgctt aaatcgcgtg cgcctgtcgc ggcaccttcg
     7561 caatccgtcg ctactggcgc ggtaaatgaa atccacacca gcccgtacag caaagacgcg
     7621 ccgctggtgg ctagcctctc tgttaaccag aaaattactg ggcgtaactc tgaaaaagac
     7681 gttcgccata tcgaaattga cttaggcgac tcgggcctgc gttaccagcc gggtgacgcg
     7741 ctgggcgtct ggtatcagaa cgatccggca ctggtgaaag aacttgtcga actgctgtgg
     7801 ctgacaggcg atgaacctgt cactgtcgag ggcaaaacgt tgcctctgaa cgaagcgcta
     7861 cagtggcact tcgaactgac cgtcaacacc gccaacattg ttgagaatta cgccacgctt
     7921 acccgcagtg aaacactgct gccgctggtg ggcgataaag cgaagttaca gcattacgcc
     7981 gcgacgacgc tgattgttga catggtgcgt ttctccccgg cacagcttga tgccgaagcg
     8041 ctaattaatc tgctgcgccc gctgaccccg cgtctctatt ccattgcctc ctcgcaggcg
     8101 gaagtcgaga acgaagtaca cgtcaccgtt ggtgtggtgc gttacgacgt ggaaggccgc
     8161 gcccgtgccg gtggtgcctc cagcttcctc gctgaccgcg tggaagaaga gggcgaagtc
     8221 cgcgtattta tcgaacataa cgataacttc cgcctgcccg ctaacctgga aaccccggtg
     8281 attatgattg gcccaggcac cggtattgcg ccgttccgcg cctttatgca gcaacgcgcc
     8341 gctgacgaag cgccaggtaa aaactggctg ttctttggca atccgcattt tacggaagac
     8401 ttcctgtatc aggtggagtg gcagcgctac gtcaaagagg gtgtgctgac gcggatcgat
     8461 ctcgcctggt cgcgcgacca aaaagaaaaa gtttacgtac aagataaact gcgcgaacag
     8521 ggcgcggagc tgtggcgctg gatcaatgac ggtgcccaca tttatgtctg cggcgacgct
     8581 aatcgcatgg cgaaagacgt tgagcaggca cttctggaag tgattgccga atttggtggc
     8641 atggacaccg aagcggcgga tgaattttta agtgagctgc gcgtagagcg ccgttatcag
     8701 cgagatgtct actaatgagc gaaaaacatc cagggccttt agtggtcgaa ggaaaactga
     8761 cagacgccga gcgcatgaag cttgaaagca actacctgcg cggcaccatt gcggaagatt
     8821 taaacgacgg tctgaccggc ggctttaagg gcgataactt cctgctgatc cgcttccacg
     8881 gcatgtatca gcaggatgac cgcgacatcc gcgccgaacg tgctgaacag aagctggagc
     8941 cgcgccacgc gatgctgttg cgctgccgtc tgccgggtgg agttatcacc accaaacagt
     9001 ggcaggcgat cgataaattt gccggtgaaa acaccatcta tggcagcatt cgcctgacca
     9061 accgccagac gtttcagttc cacggcattc tgaaaaagaa cgtcaaaccg gtgcaccaga
     9121 tgctgcactc ggtcggtctt gatgcgctgg cgactgctaa cgacatgaac cgtaacgtac
     9181 tctgcacctc gaacccttac gagtcgcagc tgcacgcaga agcgtacgag tgggcgaaga
     9241 agatttctga acatctgctg ccgcgtaccc gcgcgtatgc ggagatctgg ctcgatcagg
     9301 aaaaagtcgc caccaccgat gaagaaccga tccttggtca gacttacctg ccgcgtaagt
     9361 tcaaaaccac ggtagtgatc ccgccgcaga acgatattga tctgcacgcc aacgacatga
     9421 acttcgtggc gatcgcggaa aacggcaagc tggtgggctt caacctgctg gtgggcggtg
     9481 ggctttctat cgaacacggt aacaagaaaa cctacgcccg cacggcgagt gagtttggct
     9541 atctgccgct agagcatacg ctagcggtgg cggaagccgt cgtgacaact cagcgtgact
     9601 ggggtaaccg aaccgatcgt aaaaatgcca aaaccaaata cacactggag cgcgtggggg
     9661 ttgagacgtt taaagcggaa gtggagcgtc gcgcggggat caaatttgaa ccgatccgcc
     9721 cgtatgaatt tacaggacgc ggcgatcgta ttggctgggt taagggcatt gatgataact
     9781 ggcacctgac gctgtttatc gaaaatggtc gcatccttga ttatccgggg cgtccgctga
     9841 aaactggcct gctggagatc gcgaagatcc acaaaggtga tttccgcatt acggcgaacc
     9901 agaatctgat catcgccggt gtgccggaaa gcgagaaagc gaagatcgag aaaattgcca
     9961 aagagagcgg gttaatgaat gccgtcacgc cgcagcgtga aaactcgatg gcctgcgtgt
    10021 cattcccgac ttgcccgctg gcgatggcgg aagcggagcg tttcctgccg tcttttatcg
    10081 acaacatcga taatttaatg gtgaaacatg gtgtcagcga tgagcatatc gtgatgcgtg
    10141 taacaggctg cccgaacggt tgtggtcgcg cgatgctggc ggaagtgggc ctggtgggta
    10201 aagcaccggg tcgctacaac ctgcatcttg gcggcaaccg cattgggaca cgtatcccac
    10261 ggatgtataa agaaaacatc accgagccgg aaatcctggc gtcgcttgat gaactgatag
    10321 ggcgctgggc gaaagagcgc gaagcgggtg aaggcttcgg cgactttacg gtgcgtgcgg
    10381 gcatcattcg cccggtgctc gatccggcgc gcgatttgtg ggattaacca tcagcccggt
    10441 cttgtaggcc tgataagacg cgtgagcgtc gcatcaggca aggcaaacag tgaggaatct
    10501 atgtccaaac tcgatctaaa cgccctgaac gaactgccga aggtagatcg cattctggcg
    10561 ctggcagaaa ctaacgccga actggaaaaa ctggacgctg aaggccgcgt tgcctgggcg
    10621 ctggataatc tgcccggtga atatgtactt tcttccagct ttggcattca ggcggcggtg
    10681 agcctgcatc tggtgaatca gattcacccg gatattccgg tgatcctcac cgacaccggc
    10741 tatctgttcc cggaaaccta ccgctttatt gacgagttaa cggacaaact caagctcaac
    10801 ctgaaagtgt accgcgctac cgaaagcgcc gcctggcagg aagcacgcta cggcaaactg
    10861 tgggaacagg gcgttgaagg cattgaaaag tacaatgaca tcaacaaagt cgaaccgatg
    10921 aaccgggcgc tgaaagaact gaatgcgcaa acgtggtttg ctggcctgcg gcgtgaacaa
    10981 tccggcagcc gtgccaattt accggtgctg gcaattcagc gtggcgtatt taaagtgctg
    11041 ccgattatcg actgggataa ccgaactatt tatcagtacc tgcaaaaaca tggcctgaaa
    11101 tatcacccat tatgggacga aggatattta tcggtgggcg atacccatac aacccgtaaa
    11161 tgggaacccg gcatggcgga agaagaaacg cgtttctttg gcttaaaaag ggaatgtgga
    11221 ttacacgaag ggtaaatatt gccggataaa ttaaacgcct ctgtcagaaa tgatgggggc
    11281 gtttttattt tgcgagcagc attccggcat ttaaaggtga acatcgcagg gaaaattaac
    11341 aggtatagaa tggcgttggg tgcttgaggc tgtctggcgt taagcattcg cgaggttcca
    11401 gatggacaaa agccccaggc gatatttcta tcaacctgag gctcgcgttc gaacgtagca
    11461 attcggatgt tagtctcttc tttgcaggga ggcaagacat gctgacaaaa tatgcccttg
    11521 tggcaatcat cgtactgtgt tgtacagtac tgggattcac gttgatggta ggtgactcgt
    11581 tatgtgagtt gagtatcaga gaacgtggta tggagtttaa ggcagttctc gcttacgaat
    11641 cgaagaagta gccaccacgc gggggtaaaa ccccgcgtac tgaattgtga tggtttggtc
    11701 tcctgcaccc tttttttaaa tttatatttt ttatgtgaat atatagtctc tatttattat
    11761 tatgaatttc aaatgaaaat cattaattta ttcataatgt aaatgtttta ttggctgata
    11821 tatatcatag atagttttta aaatatgcgt aaatatcctt taagtttact gaaggataaa
    11881 aatattgtga ctttctttga tttctgggga aaaaaccgac gtggcgagaa agagggtatc
    11941 gacgactatc accttctttg ctggcatttg ctggatgtgg ccgcaatggg ctatttaatg
    12001 gttaaaagaa attgcttcgg gctggctgat tattttcgtc aattagggat ttctgacaag
    12061 gaacaggcag ctcgattttt cgcctggttg ctgtgctggc acgatattgg aaaatttgcc
    12121 cgctcttttc aacaacttta cctgccccct gaattcaaga ttcaggaagg tgccagaaaa
    12181 aattacgaaa aaatctctca ttcaacgctg ggctactggt tgtggaatca ttatttaagt
    12241 gaatgtcagg agttacttcc ttcatcttcg ctctctccgc gcaaacttag gcgagtgata
    12301 gaaatgtgga tgcccgtgac gacagggcat catggtcggc cccctgatcg tatggatgaa
    12361 ttggataatt ttctgcctga agacaaagct gcagcacgag attttctcct tgaaatcaag
    12421 ccactgtttc cgcgaataga gatccccaca ttctgggatg atgacgaggg cgttgaactt
    12481 ataaaacaac tttcctggta tatctctgca accgtcgtac ttgcagactg gacagggtcg
    12541 tcaacgcgat tttttccacg cgtcgcacag gcaatggata ttgaagatta ctggcaaaag
    12601 gctttagttc aggctcaaaa cgcattaacc gtctttccgc cacaagcaga aaccgcgcct
    12661 ttcaccggaa ttaatacgct gtttcctttt attgagaata cgacaccgtt acagcaaaag
    12721 gtgctggatc tggatatcaa tcagccagga ccacagttgt ttattctgga agatgtgacc
    12781 ggcgcaggta aaacagaagc ggcgcttatc ctggcgcaca ggttgatggc tgcgggtaac
    12841 gctcaggggc tgttttttgg cctgccaaca atggcaacgg ccaatgccat gtacgatcga
    12901 ctggtcaaaa cctggcttgc tttctattcg ccagaggtgt tccccgctcc agcggagata
    12961 aaccggcgca atacggtgcg gttttcccgg aagtaaaccg gacgcactgg atgcgatgat
    13021 gggtatcact tagaattccc cgcccctgcg gtagaactcc cagctcccat tttcaaaccc
    13081 atcaagacgc cttcgccagc tccttcacca acggtagcat tatccgcata acgtcacggc
    13141 agcgacgttc tattcttcca ggaagtgcct tatcaatatg ttgttgatta tccagtctta
    13201 cgtcatgcca gctatttccc gccgggaagg caggtgtttt tgcgcgttgc tgataaccat
    13261 ccttatttcc aagattccag ttagtcgctt ccaccgaaag tacagcaatg cccgctttgt
    13321 cgaatatttc tgcgtcatta caacccccag tgcctttcgg ataattttta ttcaaacccg
    13381 gattggtcgt tgcggctatt ccgtgactgc gtgcaattgc cagcgccctg tcgcgcgtta
    13441 atttccttac tgcttcaggg gtttttacac cgctgttgaa atacaattta tcgccaacaa
    13501 ttaagttatc gagattaatc accagcagcg tatttttctt ttcggtgtca ctcatccgct
    13561 tgagtaaatt ctcagcgcct aattttcctt cctcttcgcc gctggtcgcc acaaatcgaa
    13621 taccatactc ggtaggcgta tttttcaggc gttctgccag ttccagcatg acacctaaac
    13681 ctgcggcgtt atcatctatt ccttgtaacg tcagtccgcc gagattggca tctgcgtcag
    13741 catcgctcag cggggcgtaa gtatccagat gcgccataat gatgatctgc tgcggcgctt
    13801 tgccttcatg agcggcaatc accgtacttc ctgtcacgtt atgccagttc ttgcgattat
    13861 cgcgggcggt ataaatatac cgactattaa atgtccgaat atcactgcga taacccattt
    13921 gctgaaactg ttggcgaata taatcggcag ataacatttc tgctggagtt ccggtcatgc
    13981 gtcccggaaa gaaagtagca atatgtcgtg cctgggtatt agcaaaatcg ccaggtttag
    14041 gtgacgaggc gtgtacgggg agaataaagc atacgccgag cgccagggca gcggtacggt
    14101 ggcgcaatgc ggaaaacata gtgagtcctt aaataccatg caaatttttt taccgccata
    14161 gtatgaaact gccgctgcgc taaaacaatt tcaaatcttc ctaaacgccc gaaatccggt
    14221 gccttaagca ctttttgata ttagctttgc caaatcgtta ttccgttaag gaactactca
    14281 ttctaattgg taatttcatt cgttctctta cgctccctat agtcgaaaca tctgatggca
    14341 agaaaatagc ggtattgcaa aggaacggtt atggctcaaa aacgacttac tcacctgcgg
    14401 caactggagg cggaaagcat ccacattatt cgcgaggtgg cggcagaatt ctcaaatccg
    14461 gtgatgctct actctatcgg taaagattcc agcgtcatgc tgcatctggc gcgcaaggcg
    14521 ttttatccag gtacgctgcc tttcccgttg ctgcatgtcg ataccggctg gaaattccgc
    14581 gagatgtatg agttccgcga tcgtacggcg aaagcctacg gctgcgagct gctggtgcat
    14641 aaaaacccgg aaggcgtggc gatggggatt aatccattcg tgcacggcag cgcgaaacat
    14701 accgatatta tgaaaactga aggcctgaaa caggcgctga acaaatacgg ttttgatgcc
    14761 gccttcggtg gtgcgcgccg tgacgaagag aaatcccgcg ctaaagagcg aatctactct
    14821 ttccgtgacc gcttccatcg ctgggatccg aaaaatcagc gcccggagct gtggcacaac
    14881 tacaacgggc aaattaacaa aggcgaaagc atccgcgtct tcccgctctc taactggacc
    14941 gagcaggata tctggcaata catctggctg gaaaatatcg acattgtgcc gctgtatctc
    15001 gctgcggaac gtccggttct ggaacgcgac ggtatgttga tgatgattga tgacaaccgt
    15061 atcgacctgc aaccgggcga agtgatcaaa aaacggatgg tgcgtttccg tacgctgggc
    15121 tgctggccgc tgaccggtgc ggtggagtca aatgcacaaa cactgccgga aatcatcgaa
    15181 gagatgctgg tttccaccac cagtgaacgt cagggtcgcg tgattgaccg cgaccaggcg
    15241 ggatccatgg agctgaaaaa acgtcagggg tatttttaag atgaacaccg tacttgcaca
    15301 acaaatcgcc aatgaaggcg gcgtcgaagc ctggatgatt gcgcaacaac ataaaagcct
    15361 gctgcgtttt ctgacctgtg gtagcgtcga tgacggcaaa agtaccctga ttggtcgtct
    15421 gctgcacgat acccgccaaa tctacgaaga tcagctctca tcgctgcata acgacagtaa
    15481 gcgtcacggc acccagggcg aaaagctgga tctggctctg ctggtggacg gcctgcaagc
    15541 tgagcgcgaa cagggcatca ctattgatgt ggcctaccgc tatttctcta ccgagaagcg
    15601 taaatttatt atcgccgaca ccccagggca cgagcagtac actcgcaata tggcgactgg
    15661 cgcatcgaca tgtgaactgg cgatcttact gattgatgcc cgtaaaggcg tgctcgatca
    15721 aacccgtcgt cacagtttta tctccacact gttggggatc aaacatctgg tcgtggcgat
    15781 caacaaaatg gatctggtgg attacagtga agagacgttc acccgtattc gtgaagatta
    15841 tctgaccttt gccgggcagc tgccgggtaa tctggatatc cgctttgtgc cgctctccgc
    15901 actggaaggt gacaacgtgg cttcgcaaag tgaaagtatg ccgtggtaca gcggtctgac
    15961 actgctcgaa gtgctggaaa ccgtggagat ccagcgagtg gtggatgccc agccaatgcg
    16021 cttcccggtg cagtacgtta accgcccaaa tctcgatttt cgtggctacg ccggaatgct
    16081 ggcatccggt cgcgtggaag tcgggcaacg tgtaaaagtg ctgccctctg gtgtggaatc
    16141 aaacgtcgcg cggatcgtga cttttgatgg tgatcgcgaa gaagtctttg ccggagaagc
    16201 gatcaccctg gtgctgacgg atgagatcga tatcagccgt ggtgatctgc tgctggcggc
    16261 agacgaagcg ttaccggcgg tgcagagtgc gtcggtggat gtggtatgga tggcggaaca
    16321 gccgctttcc ccgggccaga gttatgacat caaaattgcc ggtaagaaga cgcgtgctcg
    16381 tgttgatggc attcgttatc aggttgatat taataacctt acccaacgcg aagttgaaaa
    16441 cctgccgctg aacggcatcg gcctggtgga tctcactttt gacgagccac tggtgttaga
    16501 tcgctatcag caaaatccgg ttaccggtgg gctgattttt atcgatcgcc tgagcaatgt
    16561 gaccgtgggt gccggtatgg tgcacgagcc agttagccag gcaacagctg cgtcatctga
    16621 attcagtgca ttcgaactgg aattgaatgc cctggttcgt cgccactttc cgcactgggg
    16681 cgcgcgcgat ttgctggggg ataaataatg gcgctgcatg acgaaaacgt cgtctggcat
    16741 agtcatccgg tcactgtgca acaacgcgaa ctacaccacg gtcatcgtgg tgtagtgctg
    16801 tggtttaccg gcctctccgg gtccggtaaa tcaacggtcg ccggggcgct ggaggaggcg
    16861 ttacataaac tcagcgtcag tacgtatctg ctggatggcg acaatgttcg ccacggatta
    16921 tgcagcgatc tcggttttag cgatgccgat cgtaaagaga atatccgtcg cgtcggtgaa
    16981 gtggcgaatt tgatggttga agccggactg gtggtgctga ccgcatttat ctcgccacac
    17041 cgcgccgaac gccagatggt tcgcgaacgc gtaggagaag ggcgctttat tgaagtgttt
    17101 gtcgatacgc cgctggcgat ttgcgaagcc cgcgatccaa aaggcttata taagaaagcg
    17161 cgtgccggtg aactgcgcaa ctttacggga atagattccg tttacgaagc gcctgaatcg
    17221 gcagaaattc atctcaatgg tgaacaatta gtaacaaatt tggtacagca attattagat
    17281 ctgttgagac agaacgatat tatcagatcc tgagacacca ccgggtttcc atgcccggtt
    17341 cgtcaaagta acaggatcaa atatgcgtaa tagccataac attacactaa caaataacga
    17401 cagccttact gaggatgaag aaactacatg gtcactgcct ggtgccgtgg tcggttttat
    17461 ctcctggtta tttgcgctgg cgatgccgat gttgatttat ggctctaaca cgctgttctt
    17521 ctttctctac acctggcctt tctttctggc actgatgcct gtcgcggtag tggtggggat
    17581 tgcgctgcat tcattgatgg acggaaagct acgctacagt attgttttca ccctggtgac
    17641 agtaggcatt atgtttggcg cactgtttat gtggctactg ggctaatttg tactttcttg
    17701 tctcctctgt tcataattca aaccgtaact aataatgaga ttatgttctg cacgccctgg
    17761 gtatacgtaa caatgggcaa atgtggtaca tttgcccgcg ttgtcgcggt atccccaaca
    17821 gaggatgtag agtcgtcttc ggatgcatgg gatgatgatg cagtttttca gggggcagga
    17881 tgggtaaact aacgctgctg ttgctggcta ttctggtctg gctacagtat tcgctgtggt
    17941 tcggtaagaa cggtatacat gactataccc gcgtcaatga tgatgtggcg gcactgcaag
    18001 ctacaaacgc gaaacttaaa gcgcgaaacg atcaactttt tgccgaaatt gacgatctca
    18061 atggcggcca ggaggcgctc gaagagcgtg cgcgtaatga actcagcatg accaggccgg
    18121 gcgaaacttt ttatcgtctg gtgcctgacg cgtcgaagcg cgcacagtct gcggggcaaa
    18181 acaatcgata aatcagcccg ggaattaaca tggcaaccac tcatttggat gtttgcgccg
    18241 tggttccggc ggccggattt ggccgtcgaa tgcaaacgga atgtcctaag caatatctct
    18301 caatcggtaa tcaaaccatt cttgaacact cggtgcatgc gctgctggcg catccccggg
    18361 tgacacgtgt cgtcattgcc ataagtcctg gcgatagccg ttttgcacaa cttcctctgg
    18421 cgaatcatcc gcaaatcacc gttgtagatg gcggtgatga gcgtgccgat tccgtgctgg
    18481 caggtctgaa agccgctggc gatgtgcagt gggtattggt gcatgacgcc gctcgtcctt
    18541 gtttgcatca ggatgacctt gcgcgattgt tggcgttgag cgaaaccagc cgcacgggag
    18601 ggatcctcgc cgcaccagtg cgcgatacta tgaaacgtgc cgaaccgggc aaaaatgcca
    18661 ttgctcatac cgttgatcgc aacggcttat ggcacgcgct gacgccgcaa tttttccctc
    18721 gtgagctgtt acatgactgt ctgacgcgcg ctctaaatga aggcgctacc attaccgacg
    18781 aagcctcggc gctggaatat tgcggatttc atccgcagtt ggtcgaaggc cgcgcggata
    18841 acattaaagt cacgcgcccg gaagatttgg cactggccga gttttacctc acccgaacca
    18901 tccatcagga gaatacataa tgcgaattgg acacggtttt gacgtacacg cctttggcgg
    18961 tgaaggccca attatcattg gtggcgtacg cattccttac gaaaaaggat tgctggcgca
    19021 ttctgatggc gacgtggcgc ttcatgcgtt gaccgatgca ttgcttggcg cggcggcgct
    19081 gggggatatc ggcaaactgt tcccggatac cgatccggca tttaaaggtg ccgacagccg
    19141 cgagctgcta cgcgaagcct ggcgtcgtat tcaggcgaag ggttataccc ttggcaacgt
    19201 cgatgtcact atcatcgctc aggcaccgaa gatgttgccg cacattccac aaatgcgcgt
    19261 gtttattgcc gaagatctcg gctgccatat ggatgatgtt aacgtgaaag ccactactac
    19321 ggaaaaactt ggatttaccg gacgtgggga agggattgcc tgtgaagcgg tggcgctact
    19381 cattaaggca acaaaatgat tgagtttgat aatctcactt acctccacgg taaaccgcaa
    19441 ggcaccgggc tgctgaaagc caatccggaa gactttgtgg tggtggaaga tttgggcttt
    19501 gagcctgatg gtgaaggtga gcatattctg gttagaatcc tcaaaaacgg ctgcaatacc
    19561 cgttttgtgg cggatgcact ggcgaaattc ctgaaaattc attcctgtga agtcagcttc
    19621 gctgggcaaa aagacaaaca tgctgtaacg gaacagtggt tatgtgctcg cgtgccgggc
    19681 aaggaaatgc ccgatctgag cgcctttcaa ctggaaggct gccaggtgct ggagtatgcg
    19741 cggcacaagc gcaagctgcg tttaggggcg ctgaaaggta acgcctttac cctggttctg
    19801 cgcgaagtga gcaatcgcga tgacgttgaa caacgtctga tcgatatttg cgtaaaaggt
    19861 gtaccgaact acttcggtgc ccaacgtttt gggattggcg gtagcaactt gcagggcgcg
    19921 ctgcgctggg cgcaaaccaa tactccggtg cgcgatcgca ataaacggag tttttggttg
    19981 tcggcagccc gcagtgcgtt gtttaatcag attgttgctg agcgcctcaa aaaagcagac
    20041 gttaatcaag ttgttgacgg cgatgcgcta caattagccg gacgtggtag ctggtttgta
    20101 gcaaccaccg aagaactggc ggaattacag cgtcgcgtca acgataaaga gctgatgata
    20161 accgccgcat tgccgggcag tggcgaatgg ggaactcagc gtgaagcgct ggcattcgaa
    20221 caagcagctg tcgccgcaga aactgaatta caagctttac tggtgcgcga aaaagttgaa
    20281 gccgcgcgca gagcgatgct gctgtatccg caacaattaa gctggaactg gtgggatgac
    20341 gtcaccgtag agatccgttt ctggcttccg gcgggtagtt ttgcaaccag cgttgtcagg
    20401 gaacttatca acacaacagg tgattatgcg catattgctg agtaattatg acggggtaca
    20461 tgcacccggt atacaaacgc tggcgaaagc cttgcgtgag tttgctgacg ttcaggtggt
    20521 cgcccccgat cgtaaccgca gcggcgcttc aaattctctg acactggaat cctccctgcg
    20581 cacgtttacc tttgaaaatg gtgatattgc tgtgcaaatg ggaaccccga ccgattgtgt
    20641 ctatcttggc gtgaatgctc tgatgcgtcc gcgcccggac attgttgtgt ccggaattaa
    20701 cgccgggccg aatctggggg atgatgttat ttattccggt acggtagccg ccgcgatgga
    20761 aggccgtcat ttaggttttc cggcgctggc cgtctcgctt gacgggcata aacattacga
    20821 cactgccgcg gcggtaacct gttcaatttt gcgcgcactg tgtaaagagc cgctgcgcac
    20881 cggacgtatt cttaatatta acgttcagga tttacccttg gatcaaatca aaggtattcg
    20941 cgtgacgcgc tgcggtacac gacatccggc agatcaggtg atcccgcagc aagatccgcg
    21001 cggcaatacg ctgtactgga ttggcccgcc gggcggtaaa tgtgatgccg gtccggggac
    21061 cgattttgct gcggtagatg agggctatgt ctccatcacg ccgctgcatg tggatttaac
    21121 tgcgcatagc gcgcaagatg tggtttcaga ctggttaaac agcgtgggag ttggcacgca
    21181 atggtaagca gacgcgtaca agcacttctg gatcaattac gtgcgcaagg tattcaggat
    21241 gagcaggtgc tgaatgcact tgccgccgtg ccgcgtgaaa aattcgttga tgaagcgttt
    21301 gaacaaaaag cctgggacaa tatcgccttg ccgataggtc aggggcagac aatttcgcag
    21361 ccgtatatgg tggcgcgaat gaccgaatta ctcgagctga cgccgcagtc gcgggtgctg
    21421 gaaattggca ctggttcggg atatcaaacg gcaatcctgg cgcatcttgt ccagcatgtt
    21481 tgctcggttg aacggattaa aggcttgcag tggcaggcac gtcgccgcct gaaaaatctt
    21541 gatttacata atgtttcaac ccgtcatggt gatggatggc aaggttggca ggcacgtgcg
    21601 ccgtttgacg ctatcattgt tacggcggca ccgccggaaa ttccaactgc gctaatgacg
    21661 cagctggacg aaggcgggat tctcgtctta cccgtagggg aggagcacca gtatttgaaa
    21721 cgggtgcgtc gtcggggagg cgaatttatt atcgataccg tggaggccgt gcgctttgtc
    21781 cctttagtga agggtgagct ggcttaaaac gtgaggaaat acctggattt ttcctggtta
    21841 ttttgccgta ggtcagcgta tcgtgaacat cttttccagt gttcagtagg gtgccttgca
    21901 cggtaattat gtcactggtt attaaccaat ttttcctggg ggataaatga gcgcgggaag
    21961 cccaaaattc accgttcgcc gcattgcggc tttgtcactg gtttcgctat ggctggcagg
    22021 ctgttctgac acttcaaatc caccggcacc ggtcagctcc gttaatggca atgcgcctgc
    22081 aaatactaat tctggtatgt tgattacgcc gccgccgaaa atggggacga cgtctacagc
    22141 gcagcaaccg caaattcagc cggtacagca gccacaaatt caggctactc aacaaccgca
    22201 aatccagcca gtgcagccag tagctcagca gccggtacag atggaaaacg gacgcatcgt
    22261 ctataaccgt cagtatggga acattccgaa aggcagttat agcggcagta cctataccgt
    22321 gaaaaaaggc gacacacttt tctatatcgc ctggattact ggcaacgatt tccgtgacct
    22381 tgctcagcgc aacaatattc aggcaccata cgcgctgaac gttggtcaga ccttgcaggt
    22441 gggtaatgct tccggtacgc caatcactgg cggaaatgcc attacccagg ccgacgcagc
    22501 agagcaagga gttgtgatca agcctgcaca aaattccacc gttgctgttg cgtcgcaacc
    22561 gacaattacg tattctgagt cttcgggtga acagagtgct aacaaaatgt tgccgaacaa
    22621 caagccaact gcgaccacgg tcacagcgcc tgtaacggta ccaacagcaa gcacaaccga
    22681 gccgactgtc agcagtacat caaccagtac gcctatctcc acctggcgct ggccgactga
    22741 gggcaaagtg atcgaaacct ttggcgcttc tgaggggggc aacaagggga ttgatatcgc
    22801 aggcagcaaa ggacaggcaa ttatcgcgac cgcagatggc cgcgttgttt atgctggtaa
    22861 cgcgctgcgc ggctacggta atctgattat catcaaacat aatgatgatt acctgagtgc
    22921 ctacgcccat aacgacacaa tgctggtccg ggaacaacaa gaagtgaagg cggggcaaaa
    22981 aatagcaacc atgggtagca ccggaaccag ttcaacacgc ttgcattttg aaattcgtta
    23041 caaggggaaa tccgtaaacc cgctgcgtta tttgccgcag cgataaatcg gcggaaccag
    23101 gcttttgctt gaatgttccg tcaagggatc acgggtagga gccaccttat gagtcagaat
    23161 acgctgaaag ttcatgattt aaatgaagat gcggaatttg atgagaacgg agttgaggtt
    23221 tttgacgaaa aggccttagt agaagaggaa cccagtgata acgatttggc cgaagaggaa
    23281 ctgttatcgc agggagccac acagcgtgtg ttggacgcga ctcagcttta ccttggtgag
    23341 attggttatt caccactgtt aacggccgaa gaagaagttt attttgcgcg tcgcgcactg
    23401 cgtggagatg tcgcctctcg ccgccggatg atcgagagta acttgcgtct ggtggtaaaa
    23461 attgcccgcc gttatggcaa tcgtggtctg gcgttgctgg accttatcga agagggcaac
    23521 ttggggctga tccgcgcggt agagaagttt gacccggaac gtggtttccg cttctcaaca
    23581 tacgcaacct ggtggattcg ccagacgatt gaacgggcga ttatgaacca aacccgtact
    23641 attcgtttgc cgattcacat cgtaaaggag ctgaacgttt acctgcgaac cgcacgtgag
    23701 ttgtcccata agctggacca tgaaccaagt gcggaagaga tcgcagagca actggataag
    23761 ccagttgatg acgtcagccg tatgcttcgt cttaacgagc gcattacctc ggtagacacc
    23821 ccgctgggtg gtgattccga aaaagcgttg ctggacatcc tggccgatga aaaagagaac
    23881 ggtccggaag ataccacgca agatgacgat atgaagcaga gcatcgtcaa atggctgttc
    23941 gagctgaacg ccaaacagcg tgaagtactg gcacgtcgat tcggtttgct ggggtatgaa
    24001 gcggcaacac tggaagatgt aggtcgtgaa attggcctca cccgtgaacg tgttcgccag
    24061 attcaggttg aaggcctgcg ccgtttgcgc gaaatcctgc aaacgcaggg gctgaatatc
    24121 gaagcgctgt tccgcaagta agtaagcatc tgtcagaaag gccagtctca agcgaggctg
    24181 gcctttttct tttgtttggg aagcttgtgt ttttgtcgcc tggataagac acgtcagcgt
    24241 cctacccggc aatcagcatt ctatgtgctc atcatcttgc gcaccaactg cataaacagc
    24301 tcctgctctt ctccactcaa acgccccaaa aattcctcat ccacgctgtc accaataggt
    24361 atcgccgcag caagtagctt ttctccttcg gcagtcagcc agacaaagcg tcgccgcttg
    24421 tcagcgacgt catgttcacg tctgactaag ccacgatttt ccattcttgc caacatttct
    24481 gctagcgttg ctttggtgct gactgctgct tctatcagtg ccacctgttc aataccaggt
    24541 ttatcagcaa tggcgcgcat caccgcgtac tgtggtttgg tcaggtcagg taactcgtgc
    24601 tgccagcgag ccgtgtgctg ctgaaaaagc tgtcgtaact gatggaacgc tttatttcgt
    24661 aacgccatgt aaactccggg ctaattctct gtcgctatca tagcggtttt ttagtgacga
    24721 agaagatgtt gttacttttc aatccttgcc gctgtggaaa tgaaggagta tgttaataat
    24781 aacgttcgca tacgaataat taagagaata agcaatgaaa ctgatcgtcg ggatgacagg
    24841 ggctaccggt gcgcctcttg gtgtggcatt actgcaagcg ctgcgtgaga tgccgaatgt
    24901 cgagactcat ctggtgatgt cgaagtgggc gaaaaccacc attgaactgg aaacgcctta
    24961 cagcgctcgc gatgttgctg ccctcgcaga cttcagccat aacccggcgg atcaggcggc
    25021 gatcatctca tccggttctt ttcgtaccga cggcatgatc gttattccgt gcagtatgaa
    25081 aacgctcgcc ggtatccgcg ctggttacgc cgatggcctg gtagggcgcg cggcggacgt
    25141 cgtgctcaaa gaaggccgca aactggtgct ggtgccgcgt gaaatgccgc ttagcaccat
    25201 ccatctcgaa aatatgctcg cactttcacg catgggcgtg gcgatggtgc cgccgatgcc
    25261 tgccttttat aaccatcccg aaacggtaga tgacattgtc caccatgtgg tagcccgcgt
    25321 gctggatcaa tttggcctcg aacatcccca cgccaggcgc tggcaaggat tgccgcaggc
    25381 ccggaatttt tctcaggaga atgaataatg gcatttgatg atttacgcag ctttttacag
    25441 gcgcttgatg accacggcca gttactgaaa atcagcgaag aagtgaacgc tgagccggat
    25501 ctggcagcag cagctaacgc caccgggcgt atcggcgacg gcgcgcccgc gctgtggttt
    25561 gataatattc gcggctttac cgatgcccgc gtggcgatga ataccatcgg ttcctggcag
    25621 aaccacgcga tttcccttgg cctgccacca aacaccccgg tcaaaaagca gattgatgag
    25681 tttatccgcc gctgggataa cttcccgatt gccccggagc gccgcgccaa tccagcctgg
    25741 gcgcagaaca ccgtcgatgg cgaagagatc aacctgttcg atattctgcc gctgtttcgc
    25801 ctgaatgatg gcgatggcgg tttctatctc gacaaagcgt gcgtggtttc ccgcgatccg
    25861 ctcgacccgg ataacttcgg caagcagaac gtcggcatct accgcatgga agtgaagggc
    25921 aagcgtaagc tcggcctgca accggtgccg atgcacgata tcgccctgca tctgcataaa
    25981 gcagaagagc gcggtgaaga tctgccgatt gcgatcacgc tcggtaacga tccgatcatc
    26041 acgctgatgg gggccacgcc gctgaaatac gatcagtctg agtatgaaat ggcaggcgcg
    26101 ctgcgtgaaa gcccgtaccc gatcgccacc gctccgctga ccggttttga tgtgccgtgg
    26161 ggttcagaag tgatcctcga aggggttatc gaaagccgta aacgcgaaat cgaagggccg
    26221 ttcggtgagt ttaccgggca ctactccggt gggcgcaaca tgaccgtggt gcgcatcgat
    26281 aaagtctctt atcgcagcaa accgattttc gaatcactct atctcggtat gccgtggacc
    26341 gaaatcgact acctatgact aggtaattta ttcaagataa ccgggaagag attaaaaaag
    26401 acgggaatag attggaatca acaagttagg aactcaaggt tttcaagata aagcgagtcc
    26461 aaaaatgtta aacggttcga tatcaataaa acattcaaga taactcacag gttcttacaa
    26521 tagcctttaa agaccgttca aaaacgattc aggtgtttat tatagcgctg atgggtgcaa
    26581 gcgccaaaac caatgtaatt ttagcacagc taggcacttg cactgggtta tgcgctggct
    26641 gctttctgag gtagatttaa gtgatcgccc ttagcttcgg acaacgcgta aatctctcta
    26701 accttttcgt ctggcaaatt accaaggctt aacgctatgg ctctacgaac tccaactaga
    26761 gcgtgcaact cttggatgtg ctcatccaga agaatgagta attgctccgc tccttttctc
    26821 ccaagaacat cagctacttg ttgtttttct tgtggtttaa gaaatgacaa gaacattaga
    26881 atttgagcat caacattatc cgcattctct tttttgggtt ctttggaatc ttttagttca
    26941 acatcaccct ctcccgtcat gagccattcc aaggtaacat tttcgctttc tgctatcttt
    27001 tttaactgat acgggcgtgg tatcggtgcg taatcccgcc tgttgtaata attttttaaa
    27061 gtgccttcat tgatccccca tgcccgagca gccgcatttc ttgaagggta tcgttctact
    27121 aacctaaaga ttctttcgat aatcggttct tttccatcgg caggaaaaga accttggggt
    27181 gcttttctca ttcttggttc ctttctatct aattcattga taaataataa aattaaaaaa
    27241 taaaccataa aaacaccaaa gaaccctttg catggaatct ttggtatctt aatctttatc
    27301 catacggata tccgaccgga tatccgcgcg ggtaattttg taggttaaag gaaagatgag
    27361 acaaagagaa gatagtgacc gcgattggcc ttcgctgaag attcagctgg cgctcaaaga
    27421 taacgggtgg tcttccttcc gggagctttc actccatgct ggttatgctc aggattcact
    27481 gaaagcggta gttcgcacac caaacaaaaa atacgaacgc gtaatttctg acgctatagg
    27541 tgtaccgcca gaaatcatct ggcccagccg gtatcgtaac gatggattta tcaggaaggc
    27601 gtcgtgatat gcagtcgtac gttaccgtaa atgacctgct cggcgtacca ggaatgccag
    27661 ccaccacaaa aggcatccga caggcgttac agcgtttttc tcgtgatctt ggtgacgtta
    27721 gtcgccgtcg ggaaggcaca aaagccattg agtaccacat tgattgtctg cctgaaataa
    27781 cacgaaaggc actgcgtgaa cgctatgtgg aacagctggt ggctacagag aacaatgttt
    27841 ctgaagtgaa agctgttacc agaaaaacac gcaatcctga cgctgtccag gcaatcgaag
    27901 catatcgcgg ttctccacaa ctgatggaag aacgcctgaa tgcgctgacc gaaaaccagc
    27961 gctgggtatc tgaagcaaga gctgcgctgg tggtggaagt gctgaagctg gaaagcgccg
    28021 gtaaccccgg gcgactgaaa gccattaact ttcttgttga aaaagcccgt aaaggtgagc
    28081 tgccggagcg cctgcaacag gccgcagtta acgccaatgc aaaacgtggc gctaatcgta
    28141 ctatcagccg cgatccactt taccagtggg ttctgaaata caaccagtcg cagaacgccg
    28201 ccgaacggct gctgttactg gcaccgggaa aacgcgacga aataaaaccg gaagaaatca
    28261 gctggctgcc tgaatttctg gcgcagtatc gccaggtgaa cggcagaccc atgtcagaag
    28321 cctacgagga ttttgtcgca gaatggcagc gtcgccatgc ggacgaaccg tacatgctgg
    28381 aggtgatgcc ttcctatgat gttgtccggt atgccatgaa aaaactgccg gaagtggtga
    28441 aacaaaaagg gcgcgttacc ggcagtgaat accgtcagct tgaaggattc acccgccgcg
    28501 actggacagc tatgccggtg aattatgtct ggattggtga cggtcacggt atgaagctga
    28561 aatgtgcgca tccgatccac gggcgtccat tctcaccgga agtcacgttt gtgatcgatg
    28621 gtggaacacg gtttgttgtt ggctggagtc ttgaccttgc cgaaaacgtg ttcgccgttg
    28681 ccggtgcgat acagcacggc attcgcaacc acggaaaacc gttcctgtat tactcggata
    28741 acggttccgg tgaaacggct gacatgctgg acaaagaggt cgtgggtatt ctgccacgcc
    28801 tggggattaa acaccctacc ggtattgccg gtaatccaca gggacgcggc attattgaac
    28861 gcctgaaccg gacactgccg atgcgtatag ccaggaggta tcgcacctat tttggcaagg
    28921 gtgctgaccg ggaatcgttg cgtgtactta accgtgatct gcgttcagcg tttaacgccc
    28981 tgcaacagga caagcctctg aacgaccgcc agaaagcggc aatgcgcgag ctgccatcgt
    29041 gggcggagct tattgaggct attcgtgaag gcgtggaatg gtacaacaac cgcccgcatt
    29101 ctgagctgcc gatgaaaccg aacggacggc attacagccc gacagagttc agaaaaaaac
    29161 gccaggcaga agaagacacc gaaattgagt ggctgagtga tcttgagctg cgcgacatgt
    29221 tccgcccaat ggtggaacga ccggtaagac gctgcgaaat tcagtggctg aacaatattt
    29281 actacgcgcc ggagttgcgt gacgaacacg gtcgcaaagt gcttatcagt tatgacattc
    29341 atgacgccga gcgaattacg gtacgtcgca aggacggcag ctttatctgt gaggcgatat
    29401 ggaacggcaa taaacgcgca gcctttgctg tcagtgctga atatcacaaa caacagcagc
    29461 gtattaaagg aatgcgcaaa cgcgctgagg aaaaaattcg tgatgctgaa gatgaaggta
    29521 ttcagattct ggaacacaaa caggctgaac cctggctgag taatgtttat cgcccggttg
    29581 gtaatgtggt ggcagttcag caaccggaat atgaagaaga gcatgacgaa gaatttgaac
    29641 gcgatttccg gctggggatg caaaaacttt ttgccatgca ggaagaagat gatccgctgg
    29701 cctgaataaa aaaacagtcc gcgggaacgg actgttaaat aaaggaaaca aagctgattt
    29761 gaaataaccg aggaaatact atgactgata ttgatgatgt ttgaggtgta ctggcaatag
    29821 cggacactac catttgttct ttttttaagc agccatctga tgatattttt ccctgaaggc
    29881 tgccggggag atatttgagg tgtactggca atagcggaca ctaccatttg ttcttttttt
    29941 aagcag
//
LOCUS       DAETQL010000047        29472 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.047, whole genome shotgun sequence.
ACCESSION   DAETQL010000047 DAETQL010000000
VERSION     DAETQL010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.047"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            72..353
                     /locus_tag="K0W65_002284"
     CDS             72..353
                     /locus_tag="K0W65_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBI3095531.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            319..1396
                     /locus_tag="K0W65_002285"
                     /pseudo
     CDS             319..1396
                     /locus_tag="K0W65_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(1789..2130)
                     /locus_tag="K0W65_002286"
     CDS             complement(1789..2130)
                     /locus_tag="K0W65_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBI3095532.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2143..3015)
                     /gene="copD"
                     /locus_tag="K0W65_002287"
                     /pseudo
     CDS             complement(2143..3015)
                     /gene="copD"
                     /locus_tag="K0W65_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3019..3393)
                     /gene="yobA"
                     /locus_tag="K0W65_002288"
     CDS             complement(3019..3393)
                     /gene="yobA"
                     /locus_tag="K0W65_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBI3095533.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3532..3762
                     /gene="holE"
                     /locus_tag="K0W65_002289"
     CDS             3532..3762
                     /gene="holE"
                     /locus_tag="K0W65_002289"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBI3095534.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3864..4520
                     /locus_tag="K0W65_002290"
                     /pseudo
     CDS             3864..4520
                     /locus_tag="K0W65_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            4544..5206
                     /gene="exoX"
                     /locus_tag="K0W65_002291"
     CDS             4544..5206
                     /gene="exoX"
                     /locus_tag="K0W65_002291"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBI3095535.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5203..7263)
                     /gene="ptrB"
                     /locus_tag="K0W65_002292"
     CDS             complement(5203..7263)
                     /gene="ptrB"
                     /locus_tag="K0W65_002292"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBI3095536.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7473..8132)
                     /locus_tag="K0W65_002293"
     CDS             complement(7473..8132)
                     /locus_tag="K0W65_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBI3095537.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8459..8815)
                     /locus_tag="K0W65_002294"
     CDS             complement(8459..8815)
                     /locus_tag="K0W65_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBI3095538.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            complement(8882..9173)
                     /gene="yebG"
                     /locus_tag="K0W65_002295"
                     /pseudo
     CDS             complement(8882..9173)
                     /gene="yebG"
                     /locus_tag="K0W65_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9307..10485
                     /gene="purT"
                     /locus_tag="K0W65_002296"
     CDS             9307..10485
                     /gene="purT"
                     /locus_tag="K0W65_002296"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBI3095539.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKSDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10541..11182)
                     /gene="kdgA"
                     /locus_tag="K0W65_002297"
                     /gene_synonym="eda"
     CDS             complement(10541..11182)
                     /gene="kdgA"
                     /locus_tag="K0W65_002297"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBI3095540.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11219..13030)
                     /gene="edd"
                     /locus_tag="K0W65_002298"
     CDS             complement(11219..13030)
                     /gene="edd"
                     /locus_tag="K0W65_002298"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBI3095541.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13265..14740)
                     /gene="zwf"
                     /locus_tag="K0W65_002299"
     CDS             complement(13265..14740)
                     /gene="zwf"
                     /locus_tag="K0W65_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBI3095542.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15078..15947
                     /gene="hexR"
                     /locus_tag="K0W65_002300"
                     /gene_synonym="yebK"
     CDS             15078..15947
                     /gene="hexR"
                     /locus_tag="K0W65_002300"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBI3095543.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16075..17517
                     /gene="pyk"
                     /locus_tag="K0W65_002301"
     CDS             16075..17517
                     /gene="pyk"
                     /locus_tag="K0W65_002301"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBI3095544.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17648..18619)
                     /gene="lpxM"
                     /locus_tag="K0W65_002302"
                     /gene_synonym="msbB"
     CDS             complement(17648..18619)
                     /gene="lpxM"
                     /locus_tag="K0W65_002302"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBI3095545.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18739..20061)
                     /gene="mepM"
                     /locus_tag="K0W65_002303"
     CDS             complement(18739..20061)
                     /gene="mepM"
                     /locus_tag="K0W65_002303"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBI3095546.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(20077..21009)
                     /gene="znuA"
                     /locus_tag="K0W65_002304"
     CDS             complement(20077..21009)
                     /gene="znuA"
                     /locus_tag="K0W65_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBI3095547.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21088..21843
                     /gene="znuC"
                     /locus_tag="K0W65_002305"
     CDS             21088..21843
                     /gene="znuC"
                     /locus_tag="K0W65_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBI3095548.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21840..22625
                     /gene="znuB"
                     /locus_tag="K0W65_002306"
     CDS             21840..22625
                     /gene="znuB"
                     /locus_tag="K0W65_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBI3095549.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22772..23782)
                     /gene="ruvB"
                     /locus_tag="K0W65_002307"
     CDS             complement(22772..23782)
                     /gene="ruvB"
                     /locus_tag="K0W65_002307"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBI3095550.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23791..24402)
                     /gene="ruvA"
                     /locus_tag="K0W65_002308"
     CDS             complement(23791..24402)
                     /gene="ruvA"
                     /locus_tag="K0W65_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBI3095551.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            complement(24541..24606)
                     /gene="yobI"
                     /locus_tag="K0W65_002309"
     CDS             complement(24541..24606)
                     /gene="yobI"
                     /locus_tag="K0W65_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HBI3095552.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24676..25278
                     /locus_tag="K0W65_002310"
     CDS             24676..25278
                     /locus_tag="K0W65_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HBI3095553.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(25280..25801)
                     /gene="ruvC"
                     /locus_tag="K0W65_002311"
     CDS             complement(25280..25801)
                     /gene="ruvC"
                     /locus_tag="K0W65_002311"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBI3095554.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(25836..26576)
                     /locus_tag="K0W65_002312"
     CDS             complement(25836..26576)
                     /locus_tag="K0W65_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI3095555.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(26605..27057)
                     /gene="nudB"
                     /locus_tag="K0W65_002313"
     CDS             complement(26605..27057)
                     /gene="nudB"
                     /locus_tag="K0W65_002313"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBI3095556.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(27175..28947)
                     /gene="aspS"
                     /locus_tag="K0W65_002314"
     CDS             complement(27175..28947)
                     /gene="aspS"
                     /locus_tag="K0W65_002314"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBI3095557.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            29257..>29472
                     /locus_tag="K0W65_002315"
     CDS             29257..>29472
                     /locus_tag="K0W65_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HBI3095558.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDA"
BASE COUNT         7227 a         7572 c         7235 g         7438 t
ORIGIN      
        1 ttattaaaaa tcaaccacac ccgccagtat tctgtatatt tactggcggt catatcgtga
       61 gaggtatggc aatgaatctt gtgacactca aaacgtgggg aaaactcaga tatccggata
      121 acccaccatc aatatcaacg ctgagacgat gggcaaggaa tggaaacatt tatcctgcac
      181 ctgaactaca cgggaggagt tacagggtgg ttccggaggc tttctatatc aacccaaata
      241 aggttgatac cgatataaca caccatcagc ctaatgggcg acaagggaga gacagtccgt
      301 taatggagaa gttaaaacat gcagcggaaa aaatacgatc ccaatttgcc tagaaactta
      361 acatatcgaa ggagagacaa agcatattac tggcgcaacc ctctgacgaa agaagaattt
      421 acactaggta aaatttcaag aagagatgca gtagcgcagg caattgaagc aaatcattat
      481 atatacaaaa actactctcc tgctgcccta attgaaaagc ttaaaggggt cgactcattt
      541 actatggcag gctggattga acgttacaaa acgattctta taaggagaaa agcgtccaga
      601 aatacttata aaactcgggt aaatcaactg gagacaataa aagaaaaatt aggaggggtt
      661 ttactgacag aaataaccac tcgccatatc gccgagtttc ttgatttgta gattgaagga
      721 gggaaaaaca cgatggcagg atcaatgcgt tctgtgttat ctgatatgtt ccgcgaagcc
      781 attgttgaag gacgtatatc tcaaaatcca gtaacgccaa caagagcacc gaaaatagta
      841 gttacaagag aacggctgaa actaaagaca tacaactgca tcagggaggc agcagatcaa
      901 cttccggcat ggttcccatt agctatggac ttagcccttg taacaggaca acgtcgcgaa
      961 gacataacga atatgcggtt cagtgatatt tatgatgatc gtctccacat caggcaaatt
     1021 aagacaggaa tgatgattgc tatccccctg tcactcagcc ttcctgtcgc tggtttacga
     1081 cttggtgcag tagttgaaca gtgccgcatg gtaagtaggg gggattatct aatcagtgcc
     1141 gggattagaa aaaacagccc tgacggcagc attcacccgg acggcctgac aaagaaattt
     1201 gtcgcagcca gaaaattaac aggtatcact gttcagtgaa aacccaccaa cttttcacga
     1261 aatcagaagc ctggctggac gattgtacaa agaaatatgt ggagaagaat ttgctcagcg
     1321 tctacttggc cacacatcgg agaagacaac aaaaatgtat cttgatgaga gagaaaaaac
     1381 gtacttactg ctctgatttt aacgtaaatg gattgttaaa tgtattttgg ttgtgatata
     1441 accaaaaaag accggaatac agaaattcga gtaaatttcg gggaatttcg gggagacgtt
     1501 tgcaactaat tgattttaaa tacaattaaa aaaagaccga atacgattcc tgtattcggt
     1561 ccagggaaat ggctcttggg agagagccgt gcgctaaaag ttggcattaa tgcaggctta
     1621 gttgccttgc cctttaagaa tagatgacga cgccaggttt tccagtttgc gtgcaaaatg
     1681 gtcaataaaa agcgcggtgc tcatcagctt aaatgttaaa aaccgcccgt tctggtgaaa
     1741 gaactgaggc ggttttttta ttggaaatca aaaggctatt ttaggtaatt aacagagttt
     1801 ttcagctcgt tctataaacg gtgccagact cattttttcg ccgggattgt taggatcatc
     1861 aatctgaatc accgaaatgg gttgggcatt ggtcttccca ctggcaactt ccttttgtgc
     1921 gatatcgttt aaaggatact gcacgagggt acttgggttg atgacataca aagcattacc
     1981 cggtcggcaa gtcagcatta cctcttcgcg attaaacgcc catttgtctt tacccacttc
     2041 aaaacgactc acggttatca cctgtggagc cgccagcgcc gctgcagaac tggtgagtaa
     2101 cagaaacgcc agaatacttt ttttcatcat tttcgtaatc catcaaaaag gttcgagagt
     2161 cgcaagcagg ctgatgatca ccagcactac cgcacctatc gcccattcga gtttagtcat
     2221 ccacacaaac cagggtgccg cacgatcttc atcctgcctc atgcgcggta caagaacata
     2281 acgattagcc agcgcaatga ccaccataat catcaccagg atggctttga gcaataagag
     2341 ttggccccag taagtcgtaa gcgtgggggg aaatccggta atcaacaatg cattaagcac
     2401 gccgcttgcc agtacgccga tcaccgcaaa atgcccgcac caggaaaaac gcatcagcgc
     2461 ctgaatagcc tagtgtcgcc aacggccttt gatgagttgc atacaccaga gcaccggcag
     2521 taatccccca aaccacgccg ccgcacaaat caggtgaatc gcgtgattag tctgatggat
     2581 tttcgctgtt accccttcat tcagcgtcgc ataccccact cccgccagca gaataaattg
     2641 cgcggtagtg agcataaaca gcaaacgtgg catattccgc ggttgcataa gggcgacgat
     2701 caacgttacg agggcgagaa caatttgcca taaccagacg ccaccaaact gcgtttgcaa
     2761 aacagcctgc caaatgtttg gcgaaaatac atctgtccat cccgttccca tcaacccgcc
     2821 ctgaacggcg agcattgcag tggcactgat caaactccag acggcagcgt gctgttgtaa
     2881 acgcagagaa cgcttcgtca gcaagcgacg aatcattaag ggggccagcc aggcaccata
     2941 cattgcaaag ccaaagacca gcatcaacga ggtgaaatgg ataaatcgca gcgcgatcca
     3001 ggtaaacgcc agcatgattt atttcacgct aaaggtgtag tgtcctttcg ttttatgccc
     3061 gtccaccgaa acaacatgcc agtcaacggt gtaggttccg ggtttcagcg aatcggccag
     3121 tggaacaatc agttgctttt ggtcctgctc attccgcttc gctggtaatg ttttaatatt
     3181 ttcgtttttt ggccccgtga tttttgcacc actgaatcct gtttcaacgc cttccgagaa
     3241 gtttaaagtg attgcctgcg gggcagctgt cacttgcgcg tttgccgcag gatactgatg
     3301 cgttaaatgc gcatgtgccc agacggaagg tgtcaccaac gaagtggtca ggattgccag
     3361 cgcgtagcga agggagcgtg cagttgaagc catattatct attccttttt gtaataactt
     3421 ttttacagag cataaccttg tctaatgtct gagtcgagga tcatcaattc cggcttgcca
     3481 tccaggctca ctcttagtaa cttttgcccg cgaatgatga ggagattaag aatgctgaag
     3541 aatctggcta aactggatca aacagaaatg gataaagtga atgtcgattt ggcggcggcc
     3601 ggggtggctt ttaaagaacg ctacaatatg ccggtgatcg ctgaagcggt tgaacgtgaa
     3661 cagccagaac atttgcgcag ctggtttcgc gagcggctta ttgcccaccg tttggcttcg
     3721 gtcaatctgt cacgtttacc ttacgagccc aaacttaaat aaaacttata cagagttaca
     3781 ctttcttaca taacgcctgc taaattatga gtgttttcta aaccgccccc ataatttgca
     3841 gtcattttga aaaggaagtc attatgtcgt tctggaaagt tgcagcagcg caatatgagc
     3901 ctcgtaaaac ctcactcacc gaacaggtag ctcatcatct ggagtttgtg agggccgccg
     3961 ccaggcaaca atgtcagctg ttagtttttc catcgctttc tttactgggg tgtgattatt
     4021 cgcgacgtgc ccttcctgcc ccacccgatc tttcactgtt agacccgctt tgttatgccg
     4081 caacgaccta gcgaatgacc atcattgccg gccttcccgt tgaatataac gatcgcttta
     4141 ttcgtggaat tgcggtgttc gcaccgtggc gaaaaacgcc tgggaactac catcagagtc
     4201 atggtgcatg tctgggccgt cgttccagaa ccatcacggt ggtcgatgaa caaccgcaag
     4261 gtatggatat ggacccgacc tgttcactgt ttacgacagg gcaatgtctg ggagaacccg
     4321 acctgctggc gtcggcccgt cgcttacaat ttttttcaca tcagtactcg attgccgtgc
     4381 tgatggcaaa tgcccgtggt aacagtgcac tatgggatga atatggtcgt ctcatcgttc
     4441 gcgccgatcg tggttcatta ttgttagtcg gtcagcgttc atcacagggt tggcaaggcg
     4501 atatcattcc attacgctag gctttttcgg cctggatcat gccatgttgc gcattatcga
     4561 tacagaaacc tgcggtttgc agggagggat cgttgagatt gcctctgttg atgtcattga
     4621 cggaaaaatc gtcaacccca tgagcctcct ggtgcgcccc gatcgtccta ttagtccaca
     4681 agcgatggcg attcatcgca tcaccgaagc catggtcgcc gataaaccgt ggattgaaga
     4741 tgtgatccca cactattacg gtagtgaatg gtatgtcgcg cacaacgcca gctttgaccg
     4801 ccgcgtactg cctgagatgc ccggtgagtg gatttgcact atgaaactgg cccgtcgttt
     4861 gtggccaggg ctcaagtaca gcaatatggc gttatataaa acacgcaagc tcaatgtaca
     4921 gacaccgctg ggcctgcatc atcaccgcgc gttgtatgac tgttatatca ccgccgcgtt
     4981 gcttatcgat attatgaaca cctccggctg gacggcagaa cagatggccg atatcaccgg
     5041 acgtccgtcg ttgatgacaa ccttcacctt tggcaaatac cgtggcaaag cggtttccga
     5101 cgttgccgaa cgctatccgg gctatctgcg ctggttattt aataacctgg acagcatgag
     5161 cccggagctg cgtttaacac tgaaacatta tctggaaaat acttaatccg caggcgtagc
     5221 gggtaatgtt ccctgcgcca gcgcgaccag aaaagcatat tccatcgcta cgccttcgta
     5281 cgatttaaag cggccagatt taccgccatg gcctgagtcc atgtcggtac agagcaataa
     5341 aagatggtca tcggttttca gctcgcgcaa tttagcgacc cattttgccg gttcccaata
     5401 ttgcacctga gaatcgtgta aaccggtcgt taccagtaaa tgcggataag cctgtgcggt
     5461 gacgttgtca tacgggctgt agcttttcat gtactcgtaa tattgcggat cctgcggatt
     5521 tccccactct tcaaactcac cagtggtaag aggaattgat tcatcaagca tcgttgttac
     5581 aacatcaaca aacggtacct gggcgataac gccgtggaat aattccgggc gttgattaat
     5641 tgcaacgccc atcaacatgc cccccgcact tccgcccatc gcataacaga gcgaaggaga
     5701 gccatagccc agttttaaca atgcatcgca ggcatcaaga taatcattaa acgtattttt
     5761 cttcttcaga aatttaccgt cttcgtacca ttgttgcccc agctcaccac cgccgcgaac
     5821 atggacaatg gcgtagacaa agccacgatc taacaaactc aagcggctaa aactgaaatc
     5881 ggcatcaata cttgcgccgt aagaaccata gccatacacc agcaacgggt tgtgtccttt
     5941 gcgaaaatgt ttgcgatgat agaccagcga aaccggaact tcgacgccat cacgggcgac
     6001 tatccacagg tgttcactgc ggtaattcgc cgcatcaaaa ccaggcactt ctgtttgttt
     6061 taatacacga cgctcaccgg tatccatatc cagttcaaac aaagtgtctg gtgtggtcat
     6121 ggaagaataa ccataacgca atcgcgcggt ttcaggttct ggattgtagg caatccaggt
     6181 cacataggcc ggatcatcaa aggcaatgcc aatgacttcc cgggtcttgc ggttaatttg
     6241 gcgcaaactg gttaacccgc gctgacgctc ttcaaccacc agccagtcgg taaacagcgt
     6301 aaacccttcc agcatgatgt tttcgcgtgg cggaattaac tcttcccact gttgctcatc
     6361 acgcatacgg gtacggtata agccaaagtt tttgccgtgg cgattggaac gcagataaaa
     6421 acgatgctgg tagtgatcaa ggctgtattc gtgatctttg cggcgcggca gaaaaacaaa
     6481 cggctcggca tcggccattt ccgcgtccag caggcgaact tcactggtgg tggcgctggc
     6541 cagatgaatg acgacatagt gcttcgacgt cgttttatgc aggctgacat aataggtatc
     6601 gtctttttct tcgtagatca gtgtatcttg cgatgctggc gtaccgatgg cgtgacgcca
     6661 gacctgataa ggcagcagcg tcaccggatg cttgcgaaca tagtagaaag tccaggagtc
     6721 atttgcccag acaaagctgg gttcaacgtt atccagcagt tccgggtacc agttacctgt
     6781 ttccagatta cgaaaacgaa tgccgtactg gcgtcgggaa agaaaatctt ctgccagcgc
     6841 cataatggtg ttatcgggcg taatcgccat tccgcccatc gaataaaact cactatgagc
     6901 tgcgcgcttg ttggcatcga gcaatgtttc ccactcatcc cactcttcgc tgaatgccga
     6961 ttgacgctgg tagatagcat attcacagcc tggttcataa atatgccgat agcggtagcc
     7021 attttttatg tagggcgcag aaacttctct ttgcggaatg cggtcgatga tttcctttaa
     7081 gatgcgatcc tgcaaggctt gttgtgaggc catcacccga tgaccgtaac tattttcttg
     7141 ttgcaggtag tccaggactt ttggctgaga acgcgtatcg tcccgcagcc agtagtaatt
     7201 atcgatgcgc gtatcgccat gaagcgtcat ggcgtgggga atgcgggcgg cttttggtag
     7261 catgttattg ttctttctgg ttggaaacat cttataaggg tggcaaaact caccggggat
     7321 gcaagcgaaa cagggtcgtc attgcttaga tgatgacagg taatggcgcg gatatcgaat
     7381 gttatgcaga cagagaaaat cagcctgttc aaatggctgt gcggttctgg atagcccgaa
     7441 atagtcaact tcaggctatc cagagagcgg aattattccg ccaaagtgcg tttttgctgt
     7501 tcgagatcgc gttcaatgcc ttcacgaaca tcctggggga ttttcagcgc gtcacccagt
     7561 gcattcaggt aactgcgttc cataaaatgg tcaatatcaa tagccgcgca gctcaggaaa
     7621 tagatttcca gcgcctcttc ttcattgcgg accccggtag ccaggcgttg tggatccagc
     7681 ggttgttcga ttgcctgctc aatgagtaca cgcccctgct cttccacgcc ggcttcacgc
     7741 aattgctggt cgattgccgc acgttcattg gcatcaatat gaccatcact tttagcggca
     7801 aagaccagcg caaggatcaa acgttccgta cgctcatcca gcggcgtact ttgcgcgcca
     7861 aactgcggtt cgtcctgatg cgccgcgcga attttatctt tgtatttatt ccacagcacc
     7921 gtacccgcta ccgctccgcc gccaaccagt aacgcgttgg tgccatattt cgtaagaagt
     7981 ttacgtgctg atttatttgc gaccagcagc ccagccagtc cgcctaatgc gcctgacact
     8041 aacagtttgc ccaatccctg atccgcagac gaggaggtag aagaactgct ttgcccaagc
     8101 agggattgca gttgatttaa ccagttagcc atatttgctc ctcaataacc atttttagtg
     8161 cctgtagcat agcggatgag gatgtcagaa aatgtatatc gagacaaaag atgcgcaaat
     8221 aagcggggcg gtaggccgtt attcaaagaa attcgcgcca ggtaaggtat ttactgtata
     8281 tcaggtgata agggtatttt aggtgaaagt agtttgtcgt tttgagtggc attgtttgat
     8341 gctgattgcc cgatgcgacg ctgttgcgtc ttctcaggcc tacgattgtg gtgcgttctg
     8401 ggacggacaa ggcactcgcg ccgcatccgc caacggtcaa tcgaattatc agaagccatt
     8461 aacgccgctg atattccgcc attcccgctt tgcagtccac gcagacctgg taatgaatat
     8521 cggcactttt accacgcacg gttagcggta ctgaccattt atcatcttta ccctgaatgt
     8581 cctgcaaact gacccaggcc acgggatcgg cctgaccgac aattttttga tcatctgccc
     8641 agcgcgccac gcgattttgt tgataatcac gttttacgct cgcggcaatt ccggcagcat
     8701 ccagaccttc acactttggg aaagtgaccg acttgctggt ttcattattg gcagcgaaaa
     8761 ctgatgcgca ggcagaaacc aacaaccgcc ctaaaaacgc ccctcttttt ttcatgtttt
     8821 tctccatagc acaatgattc aggagaaagc atggtacaaa ttgtcaggag cgcaagttgc
     8881 ttcaggccgc gtgtgaggca tcttcctctt cggattcagc accgacgacc tgtggggacg
     8941 gtaatttgcc ggttttcaga atggtgctca gcacctcttt ttgttctgcc agccatagcg
     9001 aaagggcttc acgttgctcg tcttccattt gcactggaga atttgtcagc taggtgtcaa
     9061 gcagatccgc cgtatcaagc attttgtcat aagcatcggc ttcctttttt gctggtaaac
     9121 gacattttct cttcgccctc acgaatgact acgtatttaa cttcaaccgc catttgcagc
     9181 ctctcataat aactgaaatt ttatacagta tatttctttt cggttgagaa atcaacatca
     9241 gcaataaaga cacacgcaaa cgttttcgtt tatactgcgc gcggaattaa tcaggggata
     9301 ttcgttatga cgttattagg cactgcgctg cgtccggcag caactcgcgt gatgttatta
     9361 ggctccggtg aactgggtaa agaagtggca atcgagtgtc agcgtctcgg cgtagaggtg
     9421 attgccgtcg atcgctatgc cgacgcacca gccatgcatg tcgcgcatcg ctcccatgtc
     9481 attaatatgc ttgatggtga tgcattacgc cgtgtggttg aactggaaaa accacattat
     9541 atcgtgccgg agatcgaagc tattgccacc gatatgctga tccaacttga agaggaagga
     9601 ctgaatgttg tcccctgcgc tcgcgcaacg aaattaacga tgaatcgcga gggtatccgt
     9661 cgcctggcgg cagaagagct gcagctgccc acttccactt atcgttttgc cgatagcaaa
     9721 agccttttcc gcgaggcggt tgccgccatt ggctatccct gcattgtaaa accggtgatg
     9781 agctcttccg gcaaggggca gacgtttatt cgctctgcag agcaacttgc tcaggcatgg
     9841 gaatacgctc agcaaggcgg tcgcgccgga gcgggccgcg taattgttga aggcgtcgtt
     9901 aagtctgact tcgaaattac cctgctaacc gtcagcgcgg tggatggcgt ccatttctgt
     9961 gcaccagtag gtcatcgcca ggaagatggc gactaccgtg aatcctggca accacagcaa
    10021 atgagcccgc ttgcccttga acgtgcgcag gagattgccc gtaaagtggt gctggcactg
    10081 ggcggttatg gtctgtttgg tgtcgagctg tttgtctgtg gtgatgaggt gattttcagt
    10141 gaggtctccc ctcgtccaca tgataccggg atggtgacgt taatttctca agatctctca
    10201 gagtttgccc tgcatgtacg tgccttcctc ggacttcctg ttggcgggat ccgtcagtat
    10261 ggtcctgcag cttctgccgt tattctgcca caactgacca gtcagaatgt cacgtttgat
    10321 aatgtgcaga atgccgtagg cgcagatttg cagattcgtt tatttggtaa gccggaaatt
    10381 gatggcagcc gtcatctggg ggtggtactg gctacttcag agagtgttgt tgacgccatt
    10441 gaacgcgcga agcacgccgc cggacaggta aaagtacagg gttaaacccg gcaaaaaaac
    10501 gctacaaaaa tgcccgatcc tagatcgggc attttgactt ttacagctta gcgccttcta
    10561 cagcttcacg cgccagctta gtaatgcggt cgtaatcgcc cgcttccagc gcatctgccg
    10621 gaaccagcca ggaaccaccg atgcacagca cgcttttcag tgccaggtag tcacggtagt
    10681 tagccggaga aataccaccc gtcgggcaga aacggacctg ggagaacgga cccgcgatcg
    10741 cctgcagggc tttcacgccg ccgttagctt cagccgggaa gaatttgaac tctttcaaac
    10801 cgtagtccat acccagcatc agttcggaaa cagtgctgat ccccggaatc agaggaatag
    10861 tcccttcggt agcagctttc agcagcggct cggtcagacc cgggctaatt gcgaactgtg
    10921 cacccgcttc agtgacttct gccagctgct gtggattcag caccgtaccg gcacccacaa
    10981 tcgcttcagg cacttctttg gcgatagcac ggatagcgtc aactgcacac tcggtacgca
    11041 gagtcacttc cagaacgcgc accccaccag caaccaacgc ttttgccatc ggcaccgcgt
    11101 gttccagttt ttttaccacg ataaccggta caaccgggcc ggtggtcagg attgattctg
    11161 cacttgtttt ccagtttttc atcagagttt tctctcgcct gattacaaat ttgtcgtctt
    11221 aaaaagtgat acaggttgcg ccctgttcgg caccggacag tttttcacgc aaggcgctga
    11281 ataattcacg tcctgttccc acgcgtgacg cgctcaggtc aggaatgtgc ggttcgcgag
    11341 cagccagttc cgcttcgtct accagcagtg tcagttcgcc tgtctgtcca ttcacacgaa
    11401 tgatgtcccc gtcgcgcact tttgccagca gcccgccatc gtaggcttct ggtgttacgt
    11461 ggatagctga cggcacttta cctgaagcgc cggagagtcg tccatcggta actaacgcaa
    11521 ttttgaaaca ccggtccaat aatacaccaa gtggcggcat gagtttatgt aattctggca
    11581 ttccgttcgc ttttggcccc tgatgacgga caacaacgac acaatcgcgg tccagcaaac
    11641 ccgcttcaaa ggccggcata acgtcatgct ggctttcaaa aacaaccgct ggcgcttcaa
    11701 tcacctggtt ctcaaccggc acggcagagg ttttcataac cgcacggccc aggttaccgc
    11761 ttaacacttt tgtcccacca tgatgagaga aaggttgttc gaaggaagcg atcacattgc
    11821 tgtcgagtga tttttccgcc ccttcccgcc agtccagttc accattattc agccatggtt
    11881 caagggtata acgagacaga ccaaagcctg ccaccgtatt gacatcttca tgcagcaggc
    11941 ctgctttgag cagttcacgc accagaaccg gtacgccacc tgccgcctgg aagtggttaa
    12001 tatcggccgg accgttcggg tagagacgtg ccatcagcgg tacaacatca gaaaggtcag
    12061 agaagtcatc ccagttaatc tgaataccgg ccgcgcgcgc catcgccacc aggtgcatgg
    12121 tgtggttagt ggaaccaccg gtcgccagca gtgcaacgat accgttcacc accactttct
    12181 catcgatcat cttaccgatc ggcatccatt cattaccatt accggtcatg cgtgtaacct
    12241 gacgcgcagc ggcagcggtc aaagcatcgc gcagcggaga atccggatga acaaaagaag
    12301 agcctggcaa ctgcatcccc ataaattcca ccaccatctg gttggtgttg gcagtaccgt
    12361 agaaagtaca tgttcccggc gcatggtaag acgcggcttc tgactccagt aaggccatgc
    12421 ggtccacttt accttcggca taaagctggc gaatacgcac tttttcttta tttggcaaac
    12481 cgcttgccat cggtccagac ggcacaaaca ccgcaggcaa atgaccaaac gacagggctg
    12541 ccatcgtcag acccgggaca atcttgtcgc acacaccgag gaacagagca ccatcaaaca
    12601 tgttatggga cagccccacc gccgcagaca tcgctatcac ttcgcggctt agcagcgaca
    12661 attccattcc atcctgcccc tgggtgacac catcacacat cgccggaaca ccgcccgcaa
    12721 cctgaccaac cgcattcgct tcatgcaggg ctttacgaat gatttctgga tagtgttcat
    12781 aaggctggtg cgcggagagc atgtcgttat aggaggtgat gatggcgata ttgttacgca
    12841 acatgctttt caaagaggct ttgttttctg gctggcaggc agcgaaaccg tgtgccaggt
    12901 taccgcatgc caactgcgaa cgatgaacgg tcgacgtttt cgcttgttct atccgggcga
    12961 gataagcaga gcgagtctcg cgcgaacgtt caatgattcg atttgttacg cgtaacaatt
    13021 gtggattcat aaaggctcct gaaattgagt tgtcagagca ggatgattca caacgcgttt
    13081 cattcagagg atttatgagt gaaacgcctg taaccggagc tcatagggca aaaacgtttc
    13141 agtcagtgta ataaaaaaag cctcgtgggt gaatccgcac gaggcctgaa agtgtaaaaa
    13201 ttgttctaca atctgtgcaa gatcatgtta ccggtaaaat aaccataaag gataagcgct
    13261 gatattactc aaactcattc caggaacgac catcacgggt aatcatcgcc accgaggcaa
    13321 cgggtcccca ggttccggcc tgatacggtt tcggcgcatc attgtccatc gcccacgcct
    13381 cagtaatgga gtctacccat ttccaggctt cttccacttc gtcgcgacgt acaaacagtg
    13441 cctgaatacc acgcatggtt tccagcagca aacgttcata ggcatccgcc agatgcgtct
    13501 gattaaaggt ttctgaatag ctcagatcca gcttggtgat ttgcaggtta tgtttgtggt
    13561 caaggccagg aactttattc agtacctgga tatccacgcc ttcatcaggt tgcagacgga
    13621 tagtcagttt attctgcggc agatcctgcc acgactcttt aaacagattc agttcaggtg
    13681 ttttgaaata gaccacgact tcagaacatt tggtcggcag acgtttacca gtacgcaggt
    13741 agaatggcac accggcccag cgccagttat caatgtcgac gcggatcgcc acgaaggttt
    13801 ctgtattgct gcttttgttc gcgccctctt cttccagata tcccggcact tttttgccct
    13861 gggcgaagcc cgcagtatat tgcccgcgta cggttttttc gcgtacgttg gagcggtcga
    13921 tgcggcgcag ggacttcagt actttcactt tttcatcgcg gatgctgtct gcgctcaggt
    13981 cagatggcgg agacatcgca atcatgcaga gaatttgtag caggtggttc tgaatcatgt
    14041 cacgcatctg accggcttta tcaaaatagc cccagcgccc ttcgatcccc acttcttctg
    14101 ccacggtaat ctcaacatga tcaatggtgc gattgtccca gttattcaca aacagggagt
    14161 tagcaaaacg cagcgccaac aggttcagca ccgtttcttt accaagatag tggtcgatac
    14221 ggtaaacctg gcactcctcg aagtattcgc caacctgatc attgatttcc tgcgaggtcg
    14281 ccagcgacgt ccccagcggt ttctccatga ctacgcgtgc cggtttagca ttcagttttg
    14341 cctcgccaag ccctttgcaa attgcgccaa aagtgctggg cggcatggca aagtagttaa
    14401 tagtgatacg atttttttga tccagcatcg cgccgagacg gttgaatgca gcagtgtcat
    14461 tgacatcgag attacaaaaa tccagacgtg cactcagggt gtcccataaa ccttcatcaa
    14521 tggtttcttt catgaaagtt tcgagcgcct cgcggacaac tttggtatat gccgctttat
    14581 cccagtcagc acgccctacg ccgataatcc gggtgtccgg gttgagctga ccgacttttt
    14641 ccagttgata cagggaaggc agcaatttac gacgcgcaag gtcgcctttc gcgccgaaaa
    14701 tgaccaggtc acaggcctgg gctgtttgcg ttaccgccat gtcattctcc ttaagttaac
    14761 taacccggta cttaagccag ggtatacttg taattttctt acggtgcact gtactgcttt
    14821 tacgagcttg cgaaaactgt aaacgcttat ccacccgtgc gattacggga aaagcgcgca
    14881 aagcgcggca aaactgataa aaaaatcatc gttttccatt cggtaaaaat ctgacactga
    14941 tcatgttatg aaaaaaaata acaacttttt tatctgcttt tgtcattaac acggcacaca
    15001 ggcgtaatat ctgacaaaac tgcatttcga tttctttcag tgcggaaatc gtcattaccc
    15061 gtgagtctct ttacatcatg aatatgctgg aaaaaatcca gtctcagctg gaacatttga
    15121 gcaaatcaga gcgcaaagtt gccgaggtca ttctggcttc gcccgataac gcgatccatt
    15181 cgagtattgc tgctctggcg ctggaagcca atgttagcga accgacggtg aatcgtttct
    15241 gtcgcagcat ggacacgcgc ggttttcctg attttaaact tcatctggca caaagtctgg
    15301 cgaatggcac tccctatgtt aatcgcaacg tcaatgaaga tgacagcgtt gaatcataca
    15361 cggggaaaat atttgagtcc gcaatggcaa cgctcgatca tgtccgtcat tcactggata
    15421 aatctgccat caaccgcgcc gtcgacttgc tcactcaggc aaaaaaaatc gcctttttcg
    15481 gattaggctc ttctgccgcc gttgcccacg atgcgatgaa taagttcttt cgttttaatg
    15541 ttccggtggt gtactccgat gatatcgtgc tgcaacgcat gagttgtatg aattgtagcg
    15601 acggagacgt ggtggtgctg atttctcaca caggaagaac aaaaaatctg gtcgagctgg
    15661 cgcagctggc acgcgaaaac gacgccatgg tgattgccct cacctctgcg ggtaccccgc
    15721 tcgcccggga agcaacgctg gcaattaccc tcgacgtacc ggaagatact gacatttata
    15781 tgcccatggt ttctcgactt gcacagctga ccgtgataga tgtgctggcg acaggattta
    15841 ctttgcgacg cggtgcaaaa ttcagagata acttgaagcg ggtcaaggaa gcgctgaagg
    15901 aatcgcgttt tgataagcag ttacttaatt taagtgacga tcgctaaaag cgactgtcac
    15961 tgtcctaatc ttatacgaca tccgaatgag attaatttat cgccatcgcg gcgttatttc
    16021 attcggattt catgttcaag caacacctgg ttgtttcagt caacggagta ttacatgtcc
    16081 aaaaggcttc gcagaacaaa aatcgttacc acgttaggcc cagcaacaga tcgcgataat
    16141 aatcttgaaa aagttatcgc ggcgggtgcc aacgttgtac gtatgaactt ttctcacggc
    16201 tcgcctgaag atcacaaaat gcgcgcggat aaagttcgtg agattgccgc aaaactgggg
    16261 cgtcatgtgg ctattctggg tgacctccag gggcccaaaa tccgtgtatc cacctttaaa
    16321 gaaggcaaag ttttcctcaa tattggggat aaattcctgc tcgacgccaa cctgggtaaa
    16381 ggtgaaggcg acaaagaaaa agtcggtatc gactacaaag gcctgcctac tgacgtcgtg
    16441 cctggtgaca tcctgctgct ggacgatggt cgcgtccagt taaaagtact ggaagttcag
    16501 ggcatgaaag tgttcaccga agtcaccgtc ggtggtcccc tctccaacaa taaaggtatc
    16561 aacaaacttg gcggcggttt gtcggctgaa gcgctgaccg aaaaagacaa agcagacatt
    16621 aagactgcgg cgttgattgg cgtagattac ctggctgtct ccttcccacg ctgtggcgaa
    16681 gatctgaact atgcccgtcg cctggcacgc gatgcaggat gtgatgcgaa aattgttgcc
    16741 aaggttgaac gtgcggaagc cgtttgcagc caggatgcaa tggatgacat catcctcgcc
    16801 tctgacgtgg taatggttgc acgtggcgac ctcggtgtgg aaattggcga cccggaactg
    16861 gtcggcattc agaaagcgtt gatccgtcgt gcgcgtcagc taaaccgagc ggtaatcacg
    16921 gcgacccaga tgatggagtc aatgattact aacccgatgc cgacgcgtgc agaagtcatg
    16981 gacgtagcaa acgccgttct ggatggtact gacgctgtga tgctgtctgc agaaactgcc
    17041 gctgggcagt atccgtcaga aactgttgca gccatggcgc gcgtttgcct gggtgcggaa
    17101 aaaatcccga gcatcaacgt ttctaaacac cgtctggacg ttcagttcga caatgtggaa
    17161 gaagctattg ccatgtcggc aatgtacgca gctaaccacc tgaagggcgt tacggcgatc
    17221 attaccatga ccgaatcggg tcgtaccgcg ctgatgacct cccgtatcag ctctggtcta
    17281 ccaattttcg ccatgtcgcg ccatgaacgt acgctgaacc tgactgctct ctatcgtggc
    17341 gttacgccgg tgccctttga tagcgctaat gacggcgtag cagctgccag cgaagcggtt
    17401 aatctgctgc gcgataaagg ttacttgatg tctggtgacc tggtgattgt cacccaaggc
    17461 gacgtgatga gtaccgtggg ttctactaat accacgcgta ttttaacggt agagtaagta
    17521 cgttgccgga tgcggcgaaa acgccacatc cggcctatag ttcaatgata gttcaacaga
    17581 tttcgaatat tctgaagcaa acttgaactt atcatcaggc gaaggcctct cctcgcgaga
    17641 ggctttttta tttgatggga taaagatctt tgcgcttata cggctggatt tcgcccggtt
    17701 tgcgagtttt cagcaatttt agtatccagg tgtattgttc tggtcgcgga ccaacaaaaa
    17761 tctcgacttc ttcattcatc cgccgctcaa tcgtatgatc atccgcctct aacagatcat
    17821 ccatcggtgg gcgcacctga atcgtcagac gatgcgtctt gccatcataa atcggaaaca
    17881 gcggtacaac gcgcgcacgg catactttca tcaaacgacc aatcgcgggc aacgtcgctt
    17941 tataggtggc aaagaaatcc acaaattcgc tgtgctctgg gccatgatcc tgatcgggta
    18001 aataatatcc ccagtacccc tgacgtaccg actggatgaa tggtttaata ccgtcatttc
    18061 tcgcatgcag acgaccgcca aagcgacgac gcaccgtgtt ccagacataa tcaaaaaccg
    18121 ggttgccctg attatggaac atcgctgcca ttttctgccc ttgcgaggcc atcagcatgg
    18181 caggaatatc gacggcccaa ccgtgcggca ccagaaagat aactttctcg ttattacgcc
    18241 gcatctcttc gatgatctcc agcccttgcc agccaacgcg cggctgaatt ttctccggcc
    18301 cgcgtattgc caactcagcc atcattgcca tcgcttgcgg cgcggtggca aacatctcat
    18361 caacaatcgc ttcgcgttca gcttcactac gttctggaaa gcagagcgac agattgatta
    18421 acgcacgacg gcgtgagctt tttcccagtc gtccggcaaa acgtcccagc cgtgccagaa
    18481 tgggatcacg gaactttggc ggcgttaaag cgatacccgc catcgctgct acacccagcc
    18541 atgctcccca gtagcgcggg tggcgaaagg atttatcaaa ctcaggaatg tattcgctat
    18601 tatttttttt cgtttccatg cttttccagt ttcggataag gcaaaaatca atctggtgat
    18661 agtgtagcgg cacaacttgc cccgcaccaa ataaaaaaag ccggtactga ctgcgtaccg
    18721 gctgcgaatg gatgttaatt aatcaaaccg tagctgcggc acaatctctt tggcctgcgc
    18781 caggaattcg cgacgatcgg agccggtcag cccttcggta cgcggcagtt ttgccgtcag
    18841 cgggtttacg gcctgctggt ttatccatac ttcatagtgc agatgcggcc cagttgaacg
    18901 tccggtatta ccggaaagcg cgatacggtc gccacgtttc accttctgtc ccggtttcac
    18961 cagaatcttg cgcaagtgca tataacgcgt ggtgtagctg cgaccatgac gaatagccac
    19021 ataataacct gctgcgccac tgcgtttggc aaccaccact tcaccgtcac ccactgaaag
    19081 cactggcgta ccttgtggca tggcgaaatc aacacctctg tgtggtgcaa cgcgacctgt
    19141 caccggatta gttcgacgcg ggttaaagtt agacgagata cggaactgtt tcgccgtcgg
    19201 gaatcgcaag aatcctttcg ccagaccagt accgttacga tcgtagaatt tgccatcctc
    19261 agcgcggatt gcgtaataat ctttaccttc tgaacgcaaa cgtacgccca gcagctggct
    19321 ttgctcacgt ttaccatcaa gcatttctcg agacattaat accgcaaatt catcgccttt
    19381 tttcagtttg cggaaatcca tttgccactg catggcttta atcactgcgc tcacttcggc
    19441 gctggttaaa ccggcgtttc tggcgctggc aacaaagctc cctccgacgg tacctttcag
    19501 cagattgtta acccactctc cttgctgcat ttcgctggtc attttaaaac cgttagcggc
    19561 agtacggtca taggttcggg tttcacgacg agacacttcc caggtgaggc gctgcagttc
    19621 gccgtccgcg gttaatgtcc aggagagttg ttgaccgatt ttcaggttac gcaattcttt
    19681 gtcggcagca gcaagttggg tgatatcacc catatcaata ccatactgat tgagaatgct
    19741 gcttagcgta tcgccagtgg aaacaacata ttcatgcacg cccgcttcac cggcgatttt
    19801 gtcatccagt tcgtcctggg gaatggcttc atcttcttgt gcagcttgat caatcggctc
    19861 actggcttca ggtaagagcg aacgaatttc gttctgttcc agctcaatgg ttttgacaat
    19921 tggcgtggca tcacggtgat aaacataggg ccgccagaca gcgacggcca gagtaagaac
    19981 ggtgagcgac cccaacataa cgcggtgtgg tcgcggtaaa ttattaaacg ccagggcgac
    20041 agagcgggct atctgttgca cgtaatcact tcctcattaa tctcctttca ggcagctcgc
    20101 atactggttg gctaattgac tcaggaattc tgaatagctt gttttaccca gtttgatatt
    20161 cgtccccagg ggatccaacg ttcccatacg aacggatgtc cctcgtgcga cgctctcaac
    20221 gaccgctggc ctgaactgtg gctcagcaaa aacgcaggtt gctttttgct caaccaactg
    20281 tgttcttatt tcatgtaaac gctgcgcgcc aggttgaatc tcagggttaa cggtaaaatg
    20341 accaagcggt gtcagtccga actgtttttc gaaatagccg taagcatcgt gaaaaacgaa
    20401 ataacctttc cccttgagcg gcgcgagctc gttaccaacc tgcgtttcgg ttgaggccaa
    20461 ttgtgcctca aaatccttca ggttggcgtc aagtttggct cgactttgcg gcataagttc
    20521 cactaatttt ccatggattg caaccgctgt agcccgcgct atctctgggg aaagccaaag
    20581 atgcatgttg aaatcgccgt gatggtgatc ttcgtcactt ttttccgcgt ggtcgtgatc
    20641 atcatcatcg ccgtgaatac ttttcatcag tagcggtttc acatcttcaa gctgcgcaat
    20701 cgttacctgc ttcgcttctg gtaatttgct taccggtttt tgcataaacg cttccatctc
    20761 cgggccaacc caaacgacta agtccgcgtt ctgtaagcgt tttacatccg atggacgcag
    20821 tgaataatca tgttctgaag cgccgtcagg cagtaaaacc tccgtttctg ttacaccatc
    20881 agcaatggca gaagcgatga acccaacggg tttaagcgaa gcgacaacgg cggcatctgc
    20941 ggcctgtgtt gcacctcccc agagagcggc ggataatgct gcgaaaagaa gcgttttttt
    21001 atgtaacata atgcgaccaa tcatcgtaat gaatatgaga agtgtgatat tataacattt
    21061 catgactact gcaagactaa aattaacatg acaagtctgg tttccctgga aaatgtctcg
    21121 gtttcttttg gccaacgccg cgtcctctct gatgtgtcgc tggaacttaa acctggaaaa
    21181 attttgactt tacttgggcc aaatggcgca ggtaagtcga cactggtacg ggtagtgctc
    21241 gggctggtaa cacccgatga aggggttatc aagcgcaacg gaaaactgcg catcggctat
    21301 gtaccgcaga agctgtatct cgacaccacg ttgccactga ccgtaaaccg ttttttacgc
    21361 ttacgccctg gtacacataa agaagatatt ttgcctgcac tgaaacgtgt ccaggccggg
    21421 cacctgatta acgcaccgat gcaaaagctc tctggtggcg aaacgcagcg tgtactatta
    21481 gcgcgagcat tgttaaattg cccgcaatta ttagtgctgg atgaacccac tcaaggcgtg
    21541 gatgtaaatg gccaagtggc gttatatgac cttattgacc agttgcgtcg tgaactggat
    21601 tgtggcgttt taatggtttc tcacgatctg catctggtga tggcaaaaac cgatgaagtg
    21661 ctgtgcctga atcaccacat ttgttgttcc ggcacaccgg aagttgtttc cctgcatccg
    21721 gagtttattt caatgtttgg tcctcgtggt gctgaacaac tgggtatcta tcgccatcat
    21781 cataatcatc gtcacgattt acagggacga attgttttgc gtcggggaaa tgatcgctca
    21841 tgattgaatt attatttccc ggttggttag ccgggatcat gctcgcctgt gccgcgggtc
    21901 cgctgggttc gtttgtagtc tggcgtcgta tgtcttattt cggtgatacg ctggctcatg
    21961 cctcattact tggtgtcgcg tttggtttgt tgctggacgt gaatccattc tatgcggtga
    22021 ttgccgttac gctgctgctg gcgggcggtc tggtatggct ggagaagcgt ccacagctgg
    22081 cgatcgacac gttattaggg attatggcgc acagtgccct gtcgctgggc ctagtggtcg
    22141 ttagtctgat gtctaacatt cgtgttgatt tgatggctta cctgttcggt gatttactgg
    22201 cagtgacgcc agaagatctc atctctattg cgattggcgt ggtcatcgtg gtggctattt
    22261 tgttctggca atggcgcaat ttgctgtcga tgacgattag cccggatctg gcgtttgttg
    22321 atggtgtgaa attacagcaa gtgaaattgt tgttgatgct ggtgacggca ttgacgattg
    22381 gtgtagcgat gaaatttgtc ggtgcgttga ttattacttc gttgctgatt attcctgcag
    22441 ctactgcgcg tcgctttgcc cgtacgccgg aacagatggc tggtgtcgct gttttggtgg
    22501 ggatggtggc agtaactggc ggtttaacct tttccgcgtt ttacgatacg ccggcgggtc
    22561 cgtcggtggt tctatgtgcg gcactgttat ttattatcag tatgatgaaa aagcaggcca
    22621 gctaatctat cgctgaacac atttgtcgga tgcggcgcga gcgccttatc cgacctacgg
    22681 ttcgctatct ctggctggcc tgataagacg cgaacagtgt cgcatcagac acactgccag
    22741 tgtcggatgc ggcgcgagcg accaatccga cttacggcat ttctggcggc gttatgccaa
    22801 agtgattcca cgcccgcgtc gtcgccatac gcccgcgcgg tgtacgctgc aaaaagcctt
    22861 gctgaatcaa ataaggttcc agtacatctt caatggtttc acgttcttcg ccaatggctg
    22921 ccgccaggtt atccagacct accggcccac caaagaactt atcgattacc gccagcaaca
    22981 atttgcggtc catataatcg aaaccttcag catcgacatt caacatatcc agcgcctgag
    23041 cagcgatatc tgccgagatg gtgccatcgt gcttcacttc ggcgaaatca cgcactcgac
    23101 gcagcagacg gttggcaata cgtggcgtac cgcgagcgcg acgagctact tcctgcgcac
    23161 cgtcatcact catctcaagc cccataaagc gtgcactgcg actgacgata tattgcagat
    23221 ccggcacctg ataaaactcc agacgttgca caataccaaa acggtcgcgc aacggtgatg
    23281 tcagcgaacc tgcgcgcgtg gttgcgccaa tcagggtaaa cggcggcaaa tcaattttaa
    23341 tggagcgtgc cgccggacct tcaccaatca tgatatccag ttggtagtct tccattgccg
    23401 ggtacagcac ttcttcaaca actggcgata aacggtggat ctcatcaata aacagcacgt
    23461 catgcggttc aaggttagtg agcatcgcag ccaaatcgcc cgccttttcc agcaccggac
    23521 cagaagtcgt gcgtaaatta acgcccattt cattggcgac aatgttggca agcgtagttt
    23581 tacccaaccc cggaggacca aaaatcaaca aatgatcgag ggcatcgccg cgcagtttcg
    23641 ctgctttgat gaaaatctcc atctgtgaac gaacctgcgg ctgaccaaca tactcttcca
    23701 gtaatttagg gcgaatggcg cgatctgcta catcttccgg caaagtggta ccggcagaaa
    23761 tcagacggtc tgcttcaatc atcctttacc tcataacgcg gcgcgtaggg cttcgcgaat
    23821 taatgtttca ctgctggtgt cagggcgagc gattttgctc accatgcggc ttgcttcttg
    23881 tggtttatag cctagcgcca ccagcgcggc aactgcttct tgttcagcat cgtcggtcgc
    23941 cgggctagca ggagacgtga gtaccaggtc ggcggctggc gtaaagagat cgccatgcaa
    24001 acctttaaat cggtctttca tttcaacaat caagcgttcg gcggtttttt tgccaatacc
    24061 cggcagtttc accagtgccc ccacttcttc acgctcaacg gcattaacga actgctgcgc
    24121 tgacattccg gagaggatcg ccagggccaa cttcgggccg acgccgttgg ttttgatcaa
    24181 ctctttgaac aacgtgcgtt cttgtttatt gttaaaaccg tacagcagtt gcgcgtcttc
    24241 acgcaccaca aagtgggtga aaacgatcgc ttcctgaccc gcttcaggga gttcataaaa
    24301 acaggtcatc ggcatatgca cttcatagcc tacgccgccc acttcaatta acaccagcgg
    24361 gggttgtttt tcaatgatga tgcctctgag tctgcctatc acatgacgct cctgcgtaat
    24421 gaatcaaaga taatgctgta tgataaaaaa atgctggata gatatccagc gaaggatgaa
    24481 gaaaacttgc gaggtgtccc gatgatctga aaaatggcac agtataattt attcaacagg
    24541 ttatattgga agcaaatatt tgaatattac atattcagtg aagaaatgcg taataaaaat
    24601 atacattgcg actcctgaaa aataattttt ttatgctatt acgtatattt gtatctattt
    24661 caatggaatg acaacgtgaa tattaattac cctgctgaat atgaaattgg agatatcgtc
    24721 tttacatgta taggtgctgc cttatttggt caaatatcag ctgcatcaaa ttgctggagt
    24781 aatcacgtcg ggatcattat cggtcataac ggtgaagact tcctggttgc agaaagccgg
    24841 gttccccttt caaccatcac cacgctatct cgttttatta aacgttcgtc taatcaacgc
    24901 tatgctataa agcgattaga cgctgggctg acagaacaac aaaaacaacg aattgttgaa
    24961 caggttccct cccggctacg caaactttac cacacgggtt ttaaatacga atcttcgcgc
    25021 cagttctgtt caaaatttgt ctttgatatt tataaagagg cgctatgtat tccggtgggt
    25081 gaaatagaga cgtttggaca actgttaaat agcaatccga atgcaaaact cactttctgg
    25141 aaattctggt tcttaggttc tattccgtgg gagcgtaaaa ccgtcacgcc agccagtttg
    25201 tggcatcatc cgggtttggt gttgattcac gcggagggag ttgaaacgcc tcagccggaa
    25261 ctgaccgagg cggtataact taacgcagtc gccctctcgt caggttcagc cgcgattcgc
    25321 tcatctgcat cgcattctga ctaacgtggc agtgggtgat ggcaatcgcc agcgcatcgg
    25381 cggcatccgc ctgtggatta gcgggcagtt tcagcaaggt gcggaccata tgctgcacct
    25441 ggcttttttc ggcactaccc atacctacca ccgtttgctt tacctgacgt gccgcgtatt
    25501 caaataccgg caattcctga ttcaccgccg caacaatcgc cacgccgcgc gcctggccca
    25561 gtttcagggc tgagtcagcg ttcttcgcca taaagacttg ttcaatggcg aaataatcag
    25621 gctggaactg ggtgatgatt tccgtcacgc ccgcatagat gagcttcaga cgagacggta
    25681 aatcatccac tttggtgcgt atgcatccgc tacccaggta ggacagttgc ctacctacct
    25741 ggcggatgac gccgtagccg gtcacgcgcg aacccgggtc aatgccgaga ataatagcca
    25801 tcacgcgtct ccgttttgct gtttagcagg catcatcaga gagtcgctgc aacctcatca
    25861 gagatttcac cgttatggta aacttcctgc acgtcgtcgc aatcttccag catatcgatc
    25921 agacgcatca gtttcggcgc ggtttctgca tccatatcag ctttggtgga cgggatcatg
    25981 gaaacttccg cgctgtctgc tttcagacct gccgcttcca gagcgtcgcg cactttaccc
    26041 atttcttccc atgcagtgta gacatcaatc gcgccgtcat catatgtcac aacgtcttca
    26101 gcacctgctt ccagagctgc ttccatgatg gtgtcttcgt cgcctttctc gaaggagatc
    26161 acgccttttt tgctgaacag ataagctacg gaaccgtcag tgccgaggtt accgccacat
    26221 ttgctgaatg cgtgacgcac ttcagcaacg gtacggttgc ggttgtcaga cagacattca
    26281 atcatgattg ccgtgccgcc aggaccgtaa ccttcgtaga tgatggtttc catgtttgca
    26341 tcatcatcac cgcccacacc acgtgcaatt gcgcggttca gtgtgtcacg ggtcatgttg
    26401 ttagacagtg ctttatccac cgccgcacgc agacgcgggt tagcgtccgg atcgccaccg
    26461 cccagcttag ccgcggttac cagctcacga atgattttag tgaagatttt accgcgctta
    26521 gcatcctgcg cagctttacg atgtctggtg ttggcccatt tactatgacc tgccataaaa
    26581 atatctccag atagccctgc ctgttcaggc agcgttaatt acaaactgtt caatcgcctg
    26641 ccggttgctc caggacttag tgagcgccgc cgcagcaggc gcatcaagcc acttgtaagc
    26701 cagatgttca gtgaaaacga tctgccgctc gtgcggaagc gcaagacaga accatgattc
    26761 cgtattacgc gtcacgcccg gcgcatagcg atgacgtaaa tgtgaaaaaa tttcaaactc
    26821 taccgtgcgc tgacagtcaa ttaaggtcag ttgttcagcg acaacatcaa tggtaacctc
    26881 ttcctttact tcgcgcatgg cagcttgcgg cgcggtttca ccctcttcca cgctgccggt
    26941 taccgactgc cagaaatcgg gatcgtcacg ccgctgcaac atcagcaccc gtttcgtatc
    27001 ttgtgcatag atgaccacta agatcgaaac gggacgctta tacactttat ccttcacgct
    27061 gcctcttcgt tgactgcctt cgctcatccc attcacatag ttatctatgc tcatgggagt
    27121 tcactcagtt gccgccttga tgcaacgcga atgatttcgt gtatttgagt catatcagtt
    27181 attctcagcc ttcttcacaa cctgaatgct cagctcagcc agtgcagtcg ggttagcaaa
    27241 gctcggtgct tcagtcatca gacacgctgc cgccgtggtt ttcgggaagg cgataacgtc
    27301 acggatattg tcggtgccag tcagcagcat ggtcagacgg tcaagaccga atgccagacc
    27361 tgcgtgcggc ggagtaccgt atttcagggc gtcgagcagg aagccgaatt tctcgcgctg
    27421 ttcctcttcg ttgataccca gaataccaaa caccgtctgc tgcatatcac cattatggat
    27481 acgtactgaa ccaccgccca cttcgtaacc attgatgacc atatcgtaag cgttcgccac
    27541 cgcattttcc ggtgcagctt tcagttctgc agcagtcata tctttcggtg aggtgaacgg
    27601 atggtgcatt gccgtcaggc cgccttcacc gtcgtcttca aacatcggga agtcgataac
    27661 ccacagcggt gcccatttgc tttcgtcggt cagaccaagg tctttaccca ctttcaggag
    27721 cagtgcgccc atcccgtcgg caacaatttt cttgttgtcg gcaccgaaga aaatcatatc
    27781 gccatcttgc gcggcagtac gatccaggat ggcttcgatg atttctgcat taaggaactt
    27841 cgctaccggg ctgttgatac cttccagacc tttcgcgcgt tcgttaactt tgatgtaagc
    27901 cagacctttc gcgccgtaga ttttaacgaa gttaccgtat tcgtcgatct gcttacgggt
    27961 cagcgatgcg ccacccggaa cacgtagagc ggcaacacgg cctttcggat cgttcgctgg
    28021 ccccgcaaat actgcaaact caacagattt cagcagatcg gcaacgtcgg tcagttccat
    28081 cgggttacgc agatccggtt tatcagaacc ataacggcgt tctgcttccg caaaggtcat
    28141 taccgggaaa tcgcccagat ccacgccctt cacttccagc cacaaatgac gcaccagcgc
    28201 ttccatcact tcacgcactt gcggcgcggt catgaaagaa gtttccacat cgatctgagt
    28261 aaattcaggc tgacgatcag cgcgcaggtc ttcgtcacgg aagcatttaa cgatctgata
    28321 gtaacggtca aaaccggaca tcatcagcag ctgtttgaac aactgtgggg attgcggcag
    28381 tgcgtagaat ttacctttgt gcacacgaga aggcaccagg tagtcacgcg cgccttccgg
    28441 cgtggctttg gtcagcatcg gagtttcgat gtcgaggaag ccgtggtcat ccataaaacg
    28501 gcgcaccagg ctggtgattt tagcgcgggt tttcaggcgt tgcgccattt ccgggcgacg
    28561 caggtcgagg tagcggtatt tcagacgcgc ttcttcggtg ttgacgtggt tagagtcaag
    28621 cggcagaaca tctgcgcggt tgatgatagt cagcgaggac gccagcactt caatttcgcc
    28681 ggtcgccata tcgcggttaa tatttttttc gtcacgcgca cgtacggtgc ccgtaacctg
    28741 aatgcagaac tcattacgca gttcagaggc cagctttaac gcgtccgcac gatccggatc
    28801 gaaaaatacc tgcacgatac cttcgcggtc gcgcatatcg atgaagatca ggctaccaag
    28861 atcacgacga cggttgaccc aaccacacag agtcacctgc tgccccacgt gggacaaacg
    28921 gagctgtcca caatattctg tacgcatgag atatccctta acttagctgc cggcggatgc
    28981 cccctgctgc gcaggtgacc aagtcgcagc gttagctgta tgtcacaact gaatgaaaaa
    29041 aggcggctat tatactggaa attctgccgc accgtaagag cctggcccgc gctggaacgc
    29101 ctcgttacca ctttatatcg ggcctgaaac cagactctac gccagtttgc tataaaggtg
    29161 ttgcccgaac tcataaaaat taacaaaatt tgtcgttccg ccatcggcta atcgcattaa
    29221 ggtgtgaggc acgattttgt tttttcagga gtcagcatgc ttgaacttaa tgctaaaacc
    29281 actgcgttgg tggtgatcga tttacaagaa ggcatcttac cttttgccgg tggtccacat
    29341 actgccgatg aggtagttaa tcgcgccggg aagctggcgg cgaaatttcg cgccagcggt
    29401 cagcccgtgt ttctggtgcg agttggctgg tctgccgatt acgccgaagc attaaaacag
    29461 ccggttgatg cc
//
LOCUS       DAETQL010000048        29128 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.048, whole genome shotgun sequence.
ACCESSION   DAETQL010000048 DAETQL010000000
VERSION     DAETQL010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29128
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.048"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="K0W65_002316"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="K0W65_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBI3095559.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="K0W65_002317"
     CDS             complement(2138..2584)
                     /locus_tag="K0W65_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBI3095560.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="K0W65_002318"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="K0W65_002318"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBI3095561.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="K0W65_002319"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="K0W65_002319"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBI3095562.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="K0W65_002320"
     CDS             complement(5347..6009)
                     /locus_tag="K0W65_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBI3095563.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="K0W65_002321"
     CDS             6182..6595
                     /locus_tag="K0W65_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBI3095564.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="K0W65_002322"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="K0W65_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBI3095565.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="K0W65_002323"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="K0W65_002323"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HBI3095566.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="K0W65_002324"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="K0W65_002324"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBI3095567.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="K0W65_002325"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="K0W65_002325"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBI3095568.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="K0W65_002326"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="K0W65_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBI3095569.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="K0W65_002327"
     tRNA            complement(9861..9948)
                     /locus_tag="K0W65_002327"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="K0W65_002328"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="K0W65_002328"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBI3095570.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="K0W65_002329"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="K0W65_002329"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HBI3095571.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="K0W65_002330"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="K0W65_002330"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HBI3095572.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="K0W65_002331"
     CDS             14005..14592
                     /locus_tag="K0W65_002331"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBI3095573.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="K0W65_002332"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="K0W65_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBI3095574.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="K0W65_002333"
     CDS             14984..15841
                     /locus_tag="K0W65_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBI3095575.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="K0W65_002334"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="K0W65_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBI3095576.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="K0W65_002335"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="K0W65_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBI3095577.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="K0W65_002336"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="K0W65_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBI3095578.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="K0W65_002337"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="K0W65_002337"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HBI3095579.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="K0W65_002338"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="K0W65_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="K0W65_002339"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="K0W65_002339"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HBI3095580.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="K0W65_002340"
     CDS             complement(22884..24023)
                     /locus_tag="K0W65_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBI3095581.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="K0W65_002341"
     CDS             complement(24069..26165)
                     /locus_tag="K0W65_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBI3095582.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="K0W65_002342"
     CDS             complement(26165..26911)
                     /locus_tag="K0W65_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBI3095583.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /locus_tag="K0W65_002343"
     CDS             complement(26908..27552)
                     /locus_tag="K0W65_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBI3095584.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="K0W65_002344"
     CDS             complement(27659..27964)
                     /locus_tag="K0W65_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095585.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="K0W65_002345"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="K0W65_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HBI3095586.1"
                     /translation="MSRKMTGFVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            28973..>29128
                     /locus_tag="K0W65_002346"
     CDS             28973..>29128
                     /locus_tag="K0W65_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3095587.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGT"
BASE COUNT         7052 a         7380 c         7648 g         7048 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagacaaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctagcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaaatcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataaaatggt
    28921 aatgctgcca acttactgat ttagtgtatg atggtgattt taaggtgctt gcgtggcttc
    28981 catttccatc agatgtcctt cctgctccgc tactgaaggc gtggtgcgta acggcaaaag
    29041 cactgccgga catcagcgct atctctgctc tcattgccgt aaaacatggc aactacagtt
    29101 cacttacacc gcctctcagc ccggtacg
//
LOCUS       DAETQL010000049        29044 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.049, whole genome shotgun sequence.
ACCESSION   DAETQL010000049 DAETQL010000000
VERSION     DAETQL010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29044
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.049"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="K0W65_002347"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="K0W65_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBI3095588.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="K0W65_002348"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="K0W65_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HBI3095589.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="K0W65_002349"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="K0W65_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBI3095590.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="K0W65_002350"
     CDS             4843..5082
                     /locus_tag="K0W65_002350"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095591.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="K0W65_002351"
     CDS             5092..5262
                     /locus_tag="K0W65_002351"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBI3095592.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6723
                     /gene="fdhF"
                     /locus_tag="K0W65_002352"
     CDS             5311..6723
                     /gene="fdhF"
                     /locus_tag="K0W65_002352"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBI3095593.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6724..6990
                     /locus_tag="K0W65_002353"
     CDS             6724..6990
                     /locus_tag="K0W65_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HBI3095594.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7084..7773
                     /locus_tag="K0W65_002354"
     CDS             7084..7773
                     /locus_tag="K0W65_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBI3095595.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="K0W65_002355"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="K0W65_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBI3095596.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="K0W65_002356"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="K0W65_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBI3095597.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="K0W65_002357"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="K0W65_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBI3095598.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="K0W65_002358"
     CDS             complement(10475..12193)
                     /locus_tag="K0W65_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBI3095599.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="K0W65_002359"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="K0W65_002359"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBI3095600.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="K0W65_002360"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="K0W65_002360"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBI3095601.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="K0W65_002361"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="K0W65_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBI3095602.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="K0W65_002362"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="K0W65_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBI3095603.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="K0W65_002363"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="K0W65_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="K0W65_002364"
     CDS             18432..18746
                     /locus_tag="K0W65_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HBI3095604.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="K0W65_002365"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="K0W65_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBI3095605.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="K0W65_002366"
     CDS             20570..21862
                     /locus_tag="K0W65_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBI3095606.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="K0W65_002367"
     CDS             complement(22016..23665)
                     /locus_tag="K0W65_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBI3095607.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="K0W65_002368"
                     /gene_synonym="yjcD"
     CDS             complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="K0W65_002368"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HBI3095608.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="K0W65_002369"
     CDS             complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="K0W65_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBI3095609.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26421..26744
                     /gene="soxS"
                     /locus_tag="K0W65_002370"
     CDS             26421..26744
                     /gene="soxS"
                     /locus_tag="K0W65_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBI3095610.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="K0W65_002371"
                     /pseudo
     CDS             complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="K0W65_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28753..29034
                     /locus_tag="K0W65_002372"
     CDS             28753..29034
                     /locus_tag="K0W65_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HBI3095611.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7283 a         7776 c         7354 g         6631 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcgctgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcgatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtaaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacactaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
    25801 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
    25861 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
    25921 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
    25981 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
    26041 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
    26101 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
    26161 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
    26221 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
    26281 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
    26341 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
    26401 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
    26461 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
    26521 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
    26581 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
    26641 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
    26701 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
    26761 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
    26821 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgctt gcgcagccag ttaacctgct
    26881 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
    26941 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
    27001 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
    27061 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
    27121 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
    27181 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
    27241 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
    27301 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
    27361 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
    27421 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
    27481 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
    27541 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
    27601 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
    27661 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
    27721 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
    27781 aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
    27841 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
    27901 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
    27961 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
    28021 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
    28081 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
    28141 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
    28201 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
    28261 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
    28321 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
    28381 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
    28441 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
    28501 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg
    28561 tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca
    28621 caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc
    28681 agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag
    28741 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
    28801 agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat
    28861 gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg
    28921 gggttgcttg gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg
    28981 atgatagaag agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta
    29041 gaag
//
LOCUS       DAETQL010000050        28676 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.050, whole genome shotgun sequence.
ACCESSION   DAETQL010000050 DAETQL010000000
VERSION     DAETQL010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28676
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.050"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..648
                     /locus_tag="K0W65_002373"
                     /pseudo
     CDS             1..648
                     /locus_tag="K0W65_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            645..1031
                     /locus_tag="K0W65_002374"
     CDS             645..1031
                     /locus_tag="K0W65_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095612.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1056..3269
                     /gene="tssI"
                     /locus_tag="K0W65_002375"
     CDS             1056..3269
                     /gene="tssI"
                     /locus_tag="K0W65_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBI3095613.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3292..3737
                     /locus_tag="K0W65_002376"
                     /pseudo
     CDS             3292..3737
                     /locus_tag="K0W65_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3759..4166
                     /locus_tag="K0W65_002377"
     CDS             3759..4166
                     /locus_tag="K0W65_002377"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HBI3095614.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4163..6451
                     /locus_tag="K0W65_002378"
     CDS             4163..6451
                     /locus_tag="K0W65_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBI3095615.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6448..7437
                     /locus_tag="K0W65_002379"
     CDS             6448..7437
                     /locus_tag="K0W65_002379"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095616.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7537..7740
                     /locus_tag="K0W65_002380"
     CDS             7537..7740
                     /locus_tag="K0W65_002380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095617.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7758..8078
                     /locus_tag="K0W65_002381"
     CDS             7758..8078
                     /locus_tag="K0W65_002381"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095618.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8144..8376)
                     /locus_tag="K0W65_002382"
                     /pseudo
     CDS             complement(8144..8376)
                     /locus_tag="K0W65_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8486..9256)
                     /gene="yafV"
                     /locus_tag="K0W65_002383"
     CDS             complement(8486..9256)
                     /gene="yafV"
                     /locus_tag="K0W65_002383"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBI3095619.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9437..9883
                     /gene="ivy"
                     /locus_tag="K0W65_002384"
     CDS             9437..9883
                     /gene="ivy"
                     /locus_tag="K0W65_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HBI3095620.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9926..12370)
                     /gene="fadE"
                     /locus_tag="K0W65_002385"
     CDS             complement(9926..12370)
                     /gene="fadE"
                     /locus_tag="K0W65_002385"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HBI3095621.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12610..13188
                     /gene="lpcA"
                     /locus_tag="K0W65_002386"
     CDS             12610..13188
                     /gene="lpcA"
                     /locus_tag="K0W65_002386"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBI3095622.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13394..14161
                     /locus_tag="K0W65_002387"
     CDS             13394..14161
                     /locus_tag="K0W65_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBI3095623.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14132..14872)
                     /locus_tag="K0W65_002388"
     CDS             complement(14132..14872)
                     /locus_tag="K0W65_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBI3095624.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15256..15777
                     /locus_tag="K0W65_002389"
                     /pseudo
     CDS             15256..15777
                     /locus_tag="K0W65_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15953..16450
                     /gene="rayT"
                     /locus_tag="K0W65_002390"
     CDS             15953..16450
                     /gene="rayT"
                     /locus_tag="K0W65_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HBI3095625.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16667..18379)
                     /locus_tag="K0W65_002391"
                     /pseudo
     CDS             complement(16667..18379)
                     /locus_tag="K0W65_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18351..19136
                     /gene="lafU"
                     /locus_tag="K0W65_002392"
     CDS             18351..19136
                     /gene="lafU"
                     /locus_tag="K0W65_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBI3095626.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19207..20262
                     /gene="dinB"
                     /locus_tag="K0W65_002393"
     CDS             19207..20262
                     /gene="dinB"
                     /locus_tag="K0W65_002393"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBI3095627.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20314..20607
                     /gene="yafN"
                     /locus_tag="K0W65_002394"
     CDS             20314..20607
                     /gene="yafN"
                     /locus_tag="K0W65_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HBI3095628.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20610..21008
                     /gene="yafO"
                     /locus_tag="K0W65_002395"
     CDS             20610..21008
                     /gene="yafO"
                     /locus_tag="K0W65_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HBI3095629.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21018..21470
                     /locus_tag="K0W65_002396"
     CDS             21018..21470
                     /locus_tag="K0W65_002396"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI3095630.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21716..22759
                     /locus_tag="K0W65_002397"
     CDS             21716..22759
                     /locus_tag="K0W65_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBI3095631.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22821..23435
                     /locus_tag="K0W65_002398"
                     /pseudo
     CDS             22821..23435
                     /locus_tag="K0W65_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23492..24949)
                     /gene="pepD"
                     /locus_tag="K0W65_002399"
     CDS             complement(23492..24949)
                     /gene="pepD"
                     /locus_tag="K0W65_002399"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBI3095632.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25211..25669
                     /gene="gpt"
                     /locus_tag="K0W65_002400"
     CDS             25211..25669
                     /gene="gpt"
                     /locus_tag="K0W65_002400"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBI3095633.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25761..27005
                     /gene="frsA"
                     /locus_tag="K0W65_002401"
     CDS             25761..27005
                     /gene="frsA"
                     /locus_tag="K0W65_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBI3095634.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27063..27464
                     /gene="crl"
                     /locus_tag="K0W65_002402"
     CDS             27063..27464
                     /gene="crl"
                     /locus_tag="K0W65_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBI3095635.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27503..28558)
                     /gene="phoE"
                     /locus_tag="K0W65_002403"
     CDS             complement(27503..28558)
                     /gene="phoE"
                     /locus_tag="K0W65_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBI3095636.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7084 a         7125 c         7608 g         6859 t
ORIGIN      
        1 acaaatattg atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat
       61 caaaatactg gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat
      121 accgttacag atgactggac tatatgggaa aaagctattt ttatgcgagc gatatattta
      181 tctgttcagc aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc
      241 gctaaaaaat ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag
      301 aatgaagact ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat
      361 gttgatttta aaaacagtac ggttcatgtt gattcagcgg gcctggaaaa agtagcgaaa
      421 tgtattaact gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt
      481 ggttacttag atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg
      541 atgaatgtgg gaatggagct ttgggaacga taccttaaaa acgcaactat aacgttgaat
      601 atcataatgc ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta
      661 ttatagtgat tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg
      721 cagttgctca atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca
      781 tcttttttat gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat
      841 ttgttggtga gtattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc
      901 aagatgttcc tattaaggaa atgttctgga atttgcaggg ggatttgaat ctgacatgtt
      961 ggtttcatta taaagacggg caatggagag tgattactta tattttttgg ccaccagggg
     1021 ctagatttta atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata
     1081 aaccagttgg aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg
     1141 gtatgagcac aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac
     1201 aggctgcggc ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag
     1261 tagctgctgc gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc
     1321 aggatgcagc ggcgtcgctt gcagccggtg ctccgggggc gcataacgtc acagtaagtg
     1381 gtagtgcggt gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg
     1441 ggaatctgtt ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg
     1501 tttcccccgc tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta
     1561 tcgaactgga cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg
     1621 tcggccatga gagccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc
     1681 tgacccatac cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg
     1741 aagtactgga tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc
     1801 gcgcctggca ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg
     1861 aatgggggat ttactggtgg tttgagcaca gcgagaacag ccacacgctg gtgctggtgg
     1921 atgccatcaa tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag
     1981 ggctgaaact ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg
     2041 gaaagtgggt gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg
     2101 tggccagtcc gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt
     2161 acttcactac gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc
     2221 caggcagccg ggcacatggg gccggtcata tccgtaccct catgaccggg tacaccttta
     2281 cgttgatgaa tcacccgacg gcagaaatta atcaggaata tctgctggta cagaccactc
     2341 tgtttttacg ggataatgcg cagcacagcg gtcaggatca gcattttacc tatgtcacca
     2401 cgtttgaact gcaccccacc agcgaagttt accgcccaca acgaaccctg agcaaacccc
     2461 acaccaaagg cccgcagagc gccatcgtca ccggcccggc ggggcaggaa atctggacgg
     2521 ataaatatgg ccgggtgaag gtgcagtttg gctgggaccg ttacggaaag aatgacgaaa
     2581 acagttcctg ctggatccgc gtcagctatc cgtgggcggg taaaggattc ggcatgatcc
     2641 agatcccgcg tattggccag gaagtactgg tggatttcaa aaacggcgat ccggatttgc
     2701 ccatcatcgt ggggcgcacc tataaccagg acaccatgcc gccgtggggg ctgccgggca
     2761 tggcgtcgca gagtgggata ttcagccact cattgcaggg agggccgact aacggaaata
     2821 tgctgcgctt cgatgataaa acgggtgcgg aagaggtgaa gttccatgcg gaaaaagacc
     2881 tcaataccac ggtaaaaaac aatgaaaccc acacggtcat ggtcgatcgc ataaaaacca
     2941 ttgtcaaaaa tgaaaccatc agcgtgggag aagaccgtaa tgccaccatc acaaaaaatg
     3001 atggtctttc cgtcaaactg gctcaaacca tcaatgtcgg aacgacatat cgtctggatg
     3061 ttggcgatca atttaccctt cgttgcggca atgcagcgct tgtattacac aaggatggct
     3121 cgattgagtt ttgcggaaaa caactgttac tgcacaccag cgatgttatg caactcattg
     3181 gcagtggcat tgatatgaat ccggatggcg gcacagccat gactgcggat gatattgccc
     3241 cgttacctac ccccgacgtt tctgaataaa aaatataaaa ggaatttttc tatgaccagc
     3301 ctttaccgta ttcaggaggg ctgctttgcc ctgcctgaaa cttttcttga tcgcaccgtc
     3361 aatatcttcg tcccatcggg caatgaaagg gcaactccca gtctgaatat tttccgcgat
     3421 acgctcaggc cagatgaaaa tctgaccacc tatattgacc gccagatagc cttgatgaaa
     3481 aaaaaaacct aggccagcac cgggtacttt cgcggggaac ggcactggcg ggaactggcg
     3541 aagctgccat gacaggtgaa cagattctcg caactcatcg ttctggaaaa acagaagtgt
     3601 accagcgtca ggccgggttt atcaccgggc cagcaaaggt actaatgttg acgctgacca
     3661 ctcagcgtcc ttttgatgat cacaccgacc agctatggac tgcatggttg actagttttc
     3721 agcctgctaa aagctaaaaa aaacagggag gttttgtcat gtatgaagcc gcacgggtgg
     3781 atgaccccat ctaccacacc agcgcccttg ccgggttttt gataggggcc atcataggta
     3841 tcgccattat tgccgtcgcc agctttgctt tttttacctg cggctttttg gccgggctga
     3901 ttttgagttt tctggctgat cagatagctt ccggagtcct gcaactgggt gaggctattg
     3961 ggcgctcgat acattccaca gtgggaaaaa ttctgaccgg ttctgataat gtcagcacca
     4021 acagtcgccc ggccgcccgg gccgtactga gtacggtgat atgcgaagac cattccccgg
     4081 aaatccgcat tgcccaaggt tccggcaaca tctacatcaa cagccagctg cccgtaagga
     4141 tgaccacact gagtgtgatg cagtgattga ggacggctcc cccaatgtct ttctcggcgg
     4201 cggtacgcag acggtactgg aaatcagccc ggaaatccct gactggctgc gtcaggtggt
     4261 ggatgtgctg tatgtggtcg ccggaatgct tggcgggctg gctggcgcct ggcgtcaggc
     4321 ggcaaagatg gggtcgaaat ttggtacaaa atgtgcggca aaatttattg gtggcgaaat
     4381 ggtcgggatg gccatctctg acacagtcat gggattgttc agcaatccgg tagatgtcac
     4441 cactgggcaa aaaattttgc tgccggagac ggattttacc ctgccaggac gcctgcctgt
     4501 cacctgttcg cgcttttacg ccagccacct ggaaactgaa ggattgctgg gcagaggatg
     4561 gcggctgaac tgggagataa acctgcgtga ggatgaaaca tatatcacct tcatcggtgt
     4621 acagggtcgg gaactgagtt atccgaaaga gatgctgata ccgggccacc agatattcga
     4681 cccggaagag cagttttacc tgagccgtct gcatgacgga tgctatgtgc tgcattacac
     4741 cgactgcagc tattacgtgt ttgatgaatt tgatgaccac ggcgtcgcgc cgttactttt
     4801 tatggagacg ccgtaccggc agcgtattgc tttcgggcgt gagaacggca ggctggtgcg
     4861 ggtggcctcc agcagcggac accatctgtt attgcaccgc acgatgacgc aagcagggga
     4921 acgcctgtcg catattgaac ttttgaaggg cgggcgtccc ggtaacctgg tggaatatcg
     4981 ttacgacgat aacgggcaac tgactggcgt ggtgaaccgg gctggtgtga cggtgcgcca
     5041 gtttgcctat gaaaatggcc tgatgacaga gcatcgcaac gcgacgggct ttacctgtac
     5101 ctaccactgg gaagagattg aaggtttccc gcgcgtggtg gaacacacga ccagcgacgg
     5161 cgaggattac cggttccact atgattttgc tggcgggcag acggtggtaa ccggcaggcc
     5221 ggagcagaag tggcagtggt ggtttgatga agagacgtat gtgactgcgc accggacgcc
     5281 gggcggcgga atgtaccgct tcacgtacaa cgaaaaccat tttccggtag cggtggaact
     5341 ccccggcgaa cgcagggtaa cgctggaata cgacaccctg agccgggtgg tgaaagagac
     5401 ggacccggca ggccgggtga cacagacgca gtggaacggc agttttgctg aaatcacgcg
     5461 ccgggcgctg gatgacgacc acgtatggaa ggcggattac aacgaacacg gccaggtgat
     5521 acgggagacg gacccggaag ggcgcgtcac gcggtacggg tatgatgatc aggggctgcc
     5581 ggagacggtc tgccaccccg gtaaacagca ggaccgttac acctggaacg cgctggggtt
     5641 actgagcagc caccgtcgca tcacgggcag tgtgcagtcc tggcagtata cgcagcgcgg
     5701 gatgctggcg cgccatacgg atgaagagaa acgggagacg cgctggcagt acaccccgga
     5761 agggctggtt gccagtctga gcaacggcaa cggggctcag taccggttca gctacgacgg
     5821 cgacggacgg ctgacgggag aacagcgccc ggacggcctg ataaggatgt ttgcgctgaa
     5881 tgcggacggg tttccggtca tcatcccgac gcagggcaca gagggcgggg tgcgcaatga
     5941 acagcaggag cgtgatgcgc tgggccgcct gctgcgcagt gacacgcagc acagtacacg
     6001 gaccttcagc tacaaccgtc ttgaccagat aacggaagtg acgctcaccc ccacggaaga
     6061 aggggaacgg ctgcaccaca tgcaggcgga cacggtgcgt tttgcgtatg accgcagcgg
     6121 gtggctgacg gcagagcaca gcgtgcacgg cagtatcaaa taccggcgcg atgcgctggg
     6181 caatcccacc gatatcacgt tgccggacgg gcagcatctc agtcatcttt actacggcag
     6241 cgggcatctg ctccagacgg cactggacgg tatcacggtc agtgaatatg agcgtgacag
     6301 cctgcaccgt caggtgatac gcacgcaggg gaaactggcg acgttcagcg gctacaacgc
     6361 cgacaaccgg ttaagctggc agcgcagcct gccaggtggc agccagaatc cacagcaggc
     6421 ggtactcccc cggcgcagaa cgacagcgtg acggcgcggg actatatctg gaacgcagac
     6481 ggtgaagtcg gcggcatcaa tgacaaactg cgcggttgcc tggtgttcag ctatgaccgc
     6541 agcggctggc tgaccagccg cacggggcag atgtatgacc atgaccatta ttattacgac
     6601 aaagcgggca acctgctgac ggatgagtat cagggagcgg tgatggataa ccgcctgccg
     6661 ggttacgggc gtgaccgcta ccgctataac gaatggggcg aactgacaga gcgcagggac
     6721 cagcagctgg agtggaacgc gcagggccag ttaacgcggg tcatcagcag caacagcgag
     6781 acgcggtatc agtacgatgc gctgggcagg cgcatcagca aggcgaccag caatctccat
     6841 accgaccggg gagaacgaag ccggacaacg tttgtgtggg aagggttccg gctgttacag
     6901 gagacaacgt ggcagggaaa gcggacatac ctgtacgatg cggaacagcc gtacacgccg
     6961 gtggctgcca tcacggggcg cggagagagc cagaaaatct ggtattacca cacggacctg
     7021 acgggcacgg tacacgaggt aaccgcgccg gacgggacgc tggtgtgggc gggctaccag
     7081 gcgggatttg gtgaaaaccg gggcgacatc agcaacagcg gggcgtactt tgagcagccg
     7141 cagcggctgc cggggcagta ctttgacgaa gaaacagggc tgcattacaa tctgttcagg
     7201 tattatgcgc cggagtgcgg gaggttcgta agccaggacc cgataggatt aaacggtggg
     7261 ttgaacttat atgcttatgc gcctaatccg ttaggttgga tcgatccgct aggactgaat
     7321 tctttaggaa tagattcaaa taatatattt agaggagatg gtaactataa aggtggcgga
     7381 attggacagc caaagagtgg cacaatatca gcacaggaca ttattgatca tctctaagaa
     7441 aaaggtgatg ggcgtttaac atcttttagt actaaaaaaa catctagttc caactctcgt
     7501 ggtgcagatt ttttctcaaa aaaccatgtc ttcaaagtgg cagtttctga tctcgatgat
     7561 ttagttaaaa ataatcagat cactgtttta acacaagaaa aagtaaaagc aatatttggt
     7621 ttagaatcaa gcaagaaaat tagaaaagaa cttggtaata ttcttcagaa tatggctagg
     7681 aataatgagg tgttagttca tgggcaaatt cttggaaata aagttagccg atgtaattag
     7741 ttagtggaga ggaagttgtg gacgaaatta gaataagaat tgctggtaat acggctgcgc
     7801 cttgtttcca tgtgctaaaa aataagggat ataaagtaac aatggaaact catgcattat
     7861 ctgacgattg tttagattgt gtatatgatt ggtatgcgga aaaaaatgga caatttttta
     7921 gtgcaacgag tccagaagag ttacttggat tagttgcaat gtgggaagtt cgtggtaatg
     7981 actggagagc taatttgaat gagataaata cttatcaaaa agccatagaa gaatccccgt
     8041 tttttgataa aaacggcaat gaaatagagt ctgactaaat tttattgata tagcaacgat
     8101 aataaccgga aatttcttcc agatctttcc ggctttttat tatttaactt tcgttcagca
     8161 cactaaacat cccgtctcgc atccccttaa ctggctgttt aacctgataa agttccctgc
     8221 gcagttcctg tacttcgttg ttatcccaag caggatcaaa catccctccg agatcttcac
     8281 ggtaacgccc gtgccgatct caaatcccgc ttttctcagc catttggcgc tgattttgat
     8341 caccggcgtg tcgctgcggt tgggcagata acccacggta cagcgacgct gtgttttgga
     8401 aatggtgact tctggcgtaa tattgtgttt aagttagggg ctttcgtaat ctagcccagc
     8461 ccccggccct aaacacaacc cccactcacc acaacctaaa ctcatccgca tcccgccatg
     8521 ccggaaactt ttctctatat tcccgcaatg ccaccatcga cagctccgca tcaatacgcg
     8581 ttgccttatg cgcgtcggcg gtagcgataa tctcgccttg cggattaatc acccggctgt
     8641 caccgcgata atggcagccg ttgccatcgc tgccgacgcg attgcatccc gccacatacg
     8701 cctgattctc aatcgcgcgg gccgtcagca atgcctgcca gtgcagagag cgcggagctg
     8761 gccagttggc gacgtacagg gcgaggtcat aatcgttgag attgcgcgac cacactggaa
     8821 aacgtaagtc gtagcacacc agcggcaaaa tacgccagcc gcgccattcc acaatcactc
     8881 gcgcattgcc cgctttataa tgcagatgct catctgccat gcggaacaga tgacgcttat
     8941 cataaaaatg taccgtgccg cccggctcaa ccagcaaaaa gcggttaacc gaaccagact
     9001 ccgtttgtaa tgcaacactg cctgcaatca gcgcattgca ctgctgcgcc ttagctgtca
     9061 tccagttcac tacgtcatct tgtgcaagcg acgaagctgc cgcttccatg gcaaagccgc
     9121 tggtaaacat ctccggtaga acgatcacat cgcgcccggt aataccttcc agttgacgat
     9181 caaaatggcg caggttggca ggaccatcca cccacaccaa cggttgctgc aaaagcgtaa
     9241 tcttcaaact aggcacggtg tacaactcct ttatgcgaag ggttttataa ctttaacacc
     9301 ttatcaggca gttgccttag cgcagaataa attgataaca aatgctgata ttggaaatat
     9361 ctgatttgca aattatcgtg ttatcgccag gctttaggag gttaataaca tgggcaggat
     9421 aagctcggga ggaatgatgt ttaaggcaat aacgacagtc gccgcactgg tcatcgccac
     9481 cagtgcaatg gcgcaggatg atttaaccat tagcagcctt gcaaagggcg aaaccaccaa
     9541 agctgcgttt aatcagatgg tgcaagggca taagctacct gcatgggtga tgaaaggcgg
     9601 tacttatact cccgcacaaa ccgtaacgtt gggagatgag acgtatcagg tgatgagcgc
     9661 gtgcaaaccg catgactgtg gctcgcaacg tatcgctgtg atgtggtccg agaaatctaa
     9721 tcagatgacg gggctgttct cggctattga tgagaaaacg tcgcaagaga aactcacctg
     9781 gctgaatgtg aacgatgcgc tttcgattga tggtaaaacg gtgctgttcg cggcgttgac
     9841 cggcagcctg gaaaaccatc cggatggctt taattttaaa taattagcgg ataaagaaac
     9901 ggagcctttc ggctccgtta ttcaattacg cggcttcaac tttccgcact ttctccggca
     9961 actttaccgg cttcgtcgcc agctcttccg gatcaaagtc atcaacgtta atactgcgca
    10021 gacggctttc ttccgctttc accagaatag cggcttcatc tttatcaatc agccccttcg
    10081 tcagcgcgtt gtgcgccagt tcatccagac gggtaaatgg cagattttta cccagctctt
    10141 tacagatccg ctgatgaatc gggtcggcgg caatcacatc caccaacgcc tcttccagta
    10201 aaccaaccgg gttatgctcg cttggcgtca ggtactgacc gcgaccaata cgtgaacgag
    10261 tggcgttcgg cacttgtaaa atcttcgcca ttttatgatc cagcttgtca gacggtgcca
    10321 gataatgacg tccggtcggg aagatcacca cattcagcag cccggcaacc acgcggttcg
    10381 ggaagttttg cagtaaattg tccattgcct gttctgcctg atacagcgca tcctgtacgc
    10441 cccagtgcac cagcggcaga tcggcttcat tacggccttc gtcgtcataa cgcttcagaa
    10501 cggcagaggc gaggtaaagc tggcttaaaa tatcccccag acgtgccgag atacgctcac
    10561 gacgtttcag gctgccgccc agcagtgcca tcgagacatc agaaagcagg gcgaggttgg
    10621 cgctcaggcg gttcaggtgc tgatagtagc gtttagtggc atcgccggtt ggcgtgctgc
    10681 tggttaaacc gcgcgtcagg cccagccaga agctgcgaac tttgttgcta ccgacgtgac
    10741 cgatatgttt gaacaacagt ttatcgaacg cgttgacgtc attgttcttc gccgcttcca
    10801 tctcttccag cacgtacgga tggcaacgaa tcgctccttg tccgaagatc atcatgctgc
    10861 gggtcagaat gttagcccct tcaacggtga tggcaatcgg tgcgccctgg taagcacgcg
    10921 ccaggaagtt gctttgcccg agcataatgc ctttaccgcc ggtaatatcc atcgcatcaa
    10981 taatcgactg ctgcccgcgg tgggtacagt gatacttaac gatagccgac agcacggcag
    11041 gtttttcgcc gagcataatg ccgtaggtaa tcagcgatgc cgcagcatcc attacataag
    11101 cgttcccggc aatacgcgcc agcggctctt caatcccttc catcttaccg atagagattt
    11161 tgaactgacg gcgaatgtga gaatacgcgc ccgttgccag cgctaccgat ttcacgccgc
    11221 cggttgagtt ggaaggcagg gtgatgccgc ggcctaccga gaggcactcc accagcatcc
    11281 gccagccttg cccggccatt ttcggcccgc cgatgatgta atcgatcggc acgaagacat
    11341 ctttaccgcg cgtcggtccg ttctggaacg gtacgttcag cgggaagtgg cgacgaccaa
    11401 tttccacgcc cggcgtggtg gttgggatca gcgcacaggt aatgcctaaa tcttctgcac
    11461 cgccgagtaa tttttccggg tcggagagtt taaacgccag cccaagcacg gtcgcaatcg
    11521 gtgccagcgt aatgtagcgt ttgttccagg tcagacgcat ccccagcacc tgctggccct
    11581 gccattcgcc catgcagaca atcccggtgt ccggaatcgc gcccgcatcg gaacccgctt
    11641 ccgggctggt cagtgcaaag caggggatct cctgaccacg cgccagacgc ggcagatagt
    11701 gatctttctg ctcgtcagtg ccgtaatgtt gcaacagttc gcccgggcct aatgagtttg
    11761 gcacgccgac ggtaatcgcc aggatcccgc tcacgccaga gagtttttgc agcacacgag
    11821 actgggcata agctgagaac tccagtccgc cgtactcttt tttgataatc atcgcgaaga
    11881 agcgatgctc tttaaggtac gcccacaact ccggtggcag atccgccagc tcatgagtaa
    11941 tctggaaatc attcgccatc cggcaggctt cttctaccgg gccgtcgaga aacgcttgct
    12001 cttcagcggt caggcgcggc tgcggatagt tatgcagctt tttccagtcc ggcttgccct
    12061 ggaacaagtc gccctcccac caggtggtgc ccgcatcaat cgcttctttc tcagtgcgcg
    12121 acatcggcgg catcacctta cggaaaccgc gaaataccgg cgcggaaatc atcgacttac
    12181 gcataggcgc aaagttaaat ggcacgagga taatggtcag aggcaccagt acccacgccg
    12241 accacagacc agcaacgccg agggcggctg tccaggcgag caaaatcaga ctgctgataa
    12301 ataagctcac gcggtgatag aacaacgcgc cgagcaggac aaccgtagcg agaatactca
    12361 aaatcatcat aacgaaaagc cccttacttg taggaggtct gaccacttgt gatgatatgg
    12421 ttgtagtgga tgtaaaaaca tttagcaatg tgtttacaat ataattacaa caaagctcac
    12481 attgttgctg tttttatccg cacttcaggt caaaaagtcc tggtcatagc acctgcccgt
    12541 acttctcgct tttggcggta tccggtacac tgcattttgt ctattacatt tatgctgaag
    12601 gatatcctca tgtaccagga tcttattcgt aacgaactga acgaagcggc ggaaacgctg
    12661 gctaactttt taaaagatga cgccaatatt cacgccattc agcgcgcggc ggtcctgtta
    12721 gcagacagct ttaaagccgg tggcaaagtg ctttcctgcg gcaacggcgg ttcccattgc
    12781 gacgctatgc actttgccga agagttgacc ggtcgctacc gtgaaaaccg tccgggctac
    12841 ccggcgattg ctatttctga cgttagtcat atttcctgcg tcggtaatga tttcggtttc
    12901 aatgatattt tctcccgcta cgttgaagcg gtaggtcgcg aaggcgatgt actgctgggg
    12961 atctccacct ccggtaactc tgcaaacgtg atcaaagcga tcgcagcggc gcgtgagaag
    13021 gggatgaaag tgatcaccct gaccggtaaa gacggcggta aaatggctgg cacggcggat
    13081 atcgaaattc gcgttccgca ctttggttat gccgaccgca ttcaggagat tcacattaaa
    13141 gtgatccata tcctgatcca gttaattgaa aaagagatgg ttaagtaagt ctggcgtagg
    13201 ccggataagg cgtttacgcc gcatccggca cttgtgcgct gatgcctgat gcgatgctga
    13261 cgcgtcttat catgcctaca aatctgtacc cgaaccgtag gccgaataat tcgtttacgc
    13321 cgcatccgac ctgaaaattc ttaaatcaat cttcgccggg gtccatgcgc tcccgctgtt
    13381 gtggaggtta cccatgtgcg aattgctcgg gatgagcgcc aacgtcccta ccgatatctg
    13441 ctttagtttc accggacttg tacagcgtgg tggtggaacc gggccacata aagatggctg
    13501 gggcattacc ttttacgaag gtaaaggctg tcgcacattt aaagatccac aacccagctt
    13561 taattccccc atcgccaaac ttgtccagga ctacccgata aaatcctgtt cggtggtggc
    13621 tcatattcgc caggctaatc ggggcgaggt ggcgctggaa aatactcacc catttacccg
    13681 cgagttatgg gggcgtaact ggacttatgc ccataacgga caactgacgg gctacaaatc
    13741 actggaaacc ggcaacttcc gcccggtcgg tgaaaccgac agcgaaaaag ccttttgctg
    13801 gctgctgcat aaattaacgc agcgttaccc gcgcacgccg ggcaacatgg cggcagtgtt
    13861 taaatatatc gcctcactgg cggatgaact gcgggcgaag ggcgttttca acatgctgct
    13921 ttcggacggg cgctatgtaa tggcgtattg ttcgactaat ttacactgga tcacccgccg
    13981 cgcgccgttt ggcgtggcaa cgttgctgga tcaggatgtg gaaatcgact tcagctcgca
    14041 gaccacaccg aatgatgtgg tcacggtgat tgcgacacag ccgctgacgg gcaatgaaac
    14101 ctggcaaaaa attatgccgg gcgaatggcg cttattttgc ctcggggagc gtgtagtttg
    14161 atgccagttg tggctgcacc acttcgtggc ttaacggctt gctgaccacg taacggccat
    14221 tggccacaga aacggtcggt ggcttacggg tttgctcaaa gtagtcgtag cccggcttca
    14281 gctgctccca gaagtcctta aagttggaat atttatggcg cttcatattg gcgtcggtca
    14341 tgcggaatgg gtaaatactc acctgcacgc tcggctgacc aaacaccagc gcaccagtaa
    14401 cgaactggaa gatctcatca ataccctgat tggtcattgc gtagcagccg atggatacac
    14461 aatcgccgtg gatcatcagg tatttccctt cataaccatg cgcacggtca taagcattgg
    14521 ggaatccaat attaatcgct ttgtagtaac ggctgtctgg ttttaactga ttacgctgga
    14581 cgctataaaa cccttccggg cttttgaaat cgccctgacg ctgttttggc cctaagccgc
    14641 cggaatattt acagatttta taactgtcga gcagttgata ttgctcgccc attttgacgt
    14701 aaagatcgag cgtacgttct tccttgaaga tctggatgta gacaggggat cccatcaact
    14761 gctgcttata ctctttgctc actggcgtgg tcgagctact gctgcccagc aaaccggcaa
    14821 acgaaacgca cgggatcaac aacatcgcaa gaattaatgc gattttacgc atactgctta
    14881 ttccttgata aaacggttac acacgccagg acggcaaaat ggatcccaaa tcggaatagt
    14941 ctggatttgg aaggctcaca ttatcaccaa aagagtttta cgcaagcctg tcggcgctgg
    15001 gtttacaaat ttagcacagg ggcagttttc aatagtccga aaagcgcatg tatcatttat
    15061 ttcctaatat tatgaaatct caatagtaat atgcaaataa gcaattaata atcccgttta
    15121 ctcctccggt cttttaaaat tctcaccttc attcagccgg tttatgtctt tcatgtcttc
    15181 gttcttgctc ggcagatttt tgcatccagg cgtgttttcg ttatgcgttt tgctcccttt
    15241 actcgccagc gccaccacaa ccgggagcag ataaaacagc atccagaatg gtttcctgcg
    15301 ccgctcaagg cgagtgacag acgctggcag gcgctggcgg aaaacaacca ctttttaagc
    15361 agcgatcacc tgcataacat caccgaagtg gcgattcacc gcctggagca gcagcttggc
    15421 aagccttacg tctggggcgg cacgcggccc gatcaaggct tcgattgtag tgggttagtt
    15481 ttttatgcct acaacaagat ccttgaggcg aaactcccgc gcacggccaa tgagatgtac
    15541 cactaccgtc gggcaacgat tgtggcgaat aacgacctgc gccggggaga tttgctattt
    15601 ttccatatcc acagccgcga gattgccgac catatggggg tgtatctggg cgacggtcag
    15661 tttattgagt cactgcgcac cggggaaacc attcgggtaa gccggttagc cgaacctttc
    15721 tggcaggacc attttttagg cgcgcgcagg attttgacgg aagagatgat tttgtaggcc
    15781 ggataaggcg tttacgccgc atccggcggt tgtgcacgga tgcctgatgc gacgctgacg
    15841 cgtcttatca tgcctacaaa tctgtgcgtt tttgcgagcc gctttcccga tataacacag
    15901 cgtcattgtc ggcctttcct tttcctctta ccgttgagaa aaaggagtcg ctatgtcgga
    15961 atatcgtcgt tattacatca aggggggaac atggtttttc acggtaaatt tacgcaatcg
    16021 tcgaagccat cttctgacca cccagttcca gacgctccgt aacgccatta ttaacgttaa
    16081 gcgagacaga ccttttgaaa ttaacgcctg ggtcgttttg ccggagcata tgcattgcat
    16141 atggacatta cccgaaagcg atgatgattt ttcctctcgc tggcgggaga ttaaaaagca
    16201 gttcacccat gcttgcggat tgaaaaatat ctggcaaccg cgtttttggg agcatgccat
    16261 tcgcaataca aaagattatc gccatcatgt tgattatatt tatataaatc cagagaaaca
    16321 tggctgggta aagcaggtga gtgattggcc attctcaacg ttccatcgcg atgtcgcgcg
    16381 agggttatat cccatcgatt gggcgggaga cataacggat ttaagtgccg gggagcgtat
    16441 cattttataa ctgtgcgctg gtgccggatg cgcttacgct tatccggcct acaaatcgtg
    16501 ctttaacggt aggccggata aggcgttcac gccgcatccg gcagttatgc gcagatgcct
    16561 gatgcgacgc tggcgcgtct tatcatgcct aaaaacttgt gcctgaacgg taggccggat
    16621 aaggcgttta cgccgcatcc ggcaattgtg caccgatgcc ggattcttac atcaacgccc
    16681 ccataatctt caactccagc tcatccggca cttcgttata cgacagcaca tgcagccccg
    16741 gcgcaaacaa ccttgcataa cgcggcagca aagggcgtag ctgcggcggc accagcagca
    16801 ccggatcttt ccccgccgct ttcatctgct ctttcacctg tggcatcgtg ctctggaact
    16861 ggttgagcat attcgggtca accggcacgc tgtcgagcat cactttgccg gcctgttgcg
    16921 cctgattcac tacgttggtc agcagatttt ccagctcatt attcagcgta tacaccgtca
    16981 gctcctgctt gcgaacgaac ggatgagtaa tgctgcgccg cagcgccagg cgcacatcgg
    17041 ccgccagcag aatatgatct ttagtcaccg tgctactggc gaccagcacg gtggcgatgg
    17101 tgacgatatc gcgcagggaa acgccttcgg tcagcagcgc ccggtacact ttcagcaatt
    17161 ggctgtaatt gagcgccgcg cttaaatcct ccgccaggcg cggtgccatc gacgacaaac
    17221 ggttatgcaa ctgcgtgatg tcatcatagt taaacaaatc aggaatatag ctgcgcacaa
    17281 tcttgttcac atgggtggca atcacactgg cgctgtcgat cacctgatac cccatattca
    17341 gagccttcgc tttctgcgca ggctggatcc aggtgaccgg tatcccgtac gccgggtcgt
    17401 tccccagcac gccgtcaatc tcgccgtagg tttcgctgga gggcagcgcc atcagtttat
    17461 ccgccggaat atccgcttca tcagcctcaa tgccgttgat gaaaatagcg tactggctgg
    17521 gcttgaggcg gaagttttcc cgaatgcgga tctccggcag cagcacgccg ttaccgtcgg
    17581 aaatcacctg ccgaacgccg cgaatccgct gggtgagcgg gttgccctgc gctttgtcca
    17641 ccagcgccac cagcttgtag ccgaggctta agctgatggg ctcgatcagc ggaatagttt
    17701 cccaactgac ctgttgttcg ctggtttcag tgatggtgcg ggtcagcgtt tcgaggcttt
    17761 tctcttccgc ctccgccgcc tgcgggcgtt tgctcatccg ccagccggta aagccaagca
    17821 gggcgctgaa cagcaaaaac ggcagatgcg gcatccccgg caccaccgcc agcacgaaca
    17881 tgatcccggt ggcggtgtag agcaccgacg ggcttgccag caactggtgg cgcacgtcat
    17941 gggcgatatc gccgttgtcg ctgacgcggg tgacgataat cgccgccgcg gtggagagca
    18001 gcagggaagg gatctgcgcc accaggccgt cgccgatggt catcagcacg tattgctgga
    18061 aggcggcgtc ggcgctcagg ttgtatttga agatgccgat acagacgccg ccgctcaggt
    18121 tgatcgccag aatcatcatc ccggcgatgg cgtctccgcg tacaaacttc gatgccccgt
    18181 ccatcgcgcc gtagaaatcg gcctcgctgg caacatcttt acgccgggtt tgcgcctgcg
    18241 cctggttgat caatccggcg ttaagatcgg cgtcaatcgc catctgtttg ccgggcatcg
    18301 cgtccagggt aaagcgggca gaaacctcgg aaatacgctc ggcctctttg gtgacgacga
    18361 taaagttgat tgtcaacagc gtcagtaaat ccgagcgtga aagcattatc gccgcgctgc
    18421 acgggcagtc gatttttaac ggtggtgggc tgtcgccgtt aaataaaatc agcccgtctc
    18481 atccgccaaa acctgccacc gtggcggtgc cggaagagac ggagaaaaaa gcgcgtgatg
    18541 tgaatgagaa aacggcgctg ctgaagaaga aatccgccac cgagcttggc gagttggcaa
    18601 ccagtatcaa caccattgcc cgtgatgcgc atatggaagc gaatctggag atggagattg
    18661 ttccccaggg attacgcgtg ctgattaaag acgaccagaa tcgcaatatg ttcgaacgcg
    18721 gcagcgcgca gattatgccg ttctttaaaa ctttgctggt ggagctcgcg ccagtgttcg
    18781 actcgctcta taacaaaatt attattaccg ggcataccga tgcgatggcc tacaaaaaca
    18841 atatctacaa caactggaac ctttcgggcg atcgcgcgct ttcagctcgt cgggtgctgg
    18901 aagaggccgg aatgccggaa gataaagtga tgcaggtaag cgcaatggcg gaccagatgc
    18961 tgctggacgc gaagaatccg caaagcgcgg gcaatcggcg tattgagatt atggtgctga
    19021 ccaaaagtgc gtccgatacg ctgtatcaat actttggtca gcatggggat aaagtggtgc
    19081 agccgctggt gcaaaagctg gataagcagc aggtgctttc gcagcgaatg cgctaaatgc
    19141 tgaatcttta cgcatttctc aaaacctgaa atcactgtat actttaccag tgttgagagg
    19201 tgagcaatgc gtaaaatcat tcatgtggat atggactgct ttttcgcagc ggtggagatg
    19261 cgcgacaatc ccgctctgcg cgatatccct attgctattg gcggcagccg cgaacgtcgg
    19321 ggggtgatca gcaccgccaa ttatcccgcg cgtaaatttg gcgtacgtag cgctatgcct
    19381 actgggatgg cgctcaaatt atgcccacat ctcaccttgc ttccggggcg ctttgacgcc
    19441 tacaaagaag cctcaaatca tatccgtgaa atcttctcgc gctacacctc gcgcattgaa
    19501 ccgttgtcac tggatgaggc ttatctcgat gtcaccgata gcgtccattg ccacggttct
    19561 gcgaccctca tcgcccagga aatccgccag acgatcttca acgagctgca actgacggca
    19621 tctgcgggcg tggcaccagt aaagtttctc gccaaaatcg cctccgacat gaataaaccc
    19681 aacggtcagt ttgtgattac gccggcagaa gttccggcat ttttacaaac cttaccactg
    19741 gaaaaaatcc ccggcgtcgg caaagtctcg gcggcaaaac tggaagcgat ggggctgcgg
    19801 acctgcggtg atgtacaaaa gtgtgatctg gtgattctgc ttaaacgctt tggcaaattt
    19861 ggccgcattt tgtgggagcg tagtcagggg attgacgagc gcgacgttaa cagcgaacgg
    19921 ttacgaaaat ccgtcggcgt ggaacgcacg atggcggaag atattcatca ctggtctgaa
    19981 tgtgaagcga ttatcgagcg gctgtatccg gaacttgaac gccgtctggc aaaggtaaaa
    20041 cctgatttac tgattgctcg ccagggggtg aaattaaagt tcgacgattt tcagcagacc
    20101 acccaggagc acgtctggcc gcggctgaat aaagctgact taatcgccac cgcgcgtaaa
    20161 acctgggatg aacgccgcgg cgggcgcggt gtgcgtctgg tggggctgca tgtgacgttg
    20221 cttgacccgc aaatggaaag acaactggtg ctgggattat gatgtatact attatgtata
    20281 ttctggtgtg cattattatg agggtatcac tgtatgcatc gaattctcgc tgaaaaatcg
    20341 gtcaatatca ctgagttacg taaaaaccca gctaaatact ttattgatca accagttgcg
    20401 gttctttcta ataatcgccc cgcaggatat ctcttaagtg ccagcgcatt cgaagcgtta
    20461 atggacatgc ttgctgaaca agaggagaaa aagcccataa aggcgcgctt tcgtccaagt
    20521 gctgcaagat tagaggaaat tacacgccgc gctaaacaat atcttaatga tatgacggat
    20581 gatgatttca atgactttaa ggaataagga tgcgggtatt caaaacaaaa cttattcgcc
    20641 tgcaacttac agcagaggaa cttgatgcgt taacggcgga ttttatttcc tataagcgtg
    20701 acggtgtttt gccagatata tttggtcgcg atgcactcta cgacgactcg tttacctggc
    20761 cattaatcaa atttgagcga gttgctcata ttcatctggc aaatgtgaat aatccatttc
    20821 cgccacagtt gcgccaattc agcagaacga atgacgaagc gcatttggta tattgtcagg
    20881 gtgcgtttga tgagcaagca tggttgctca ttgccattct gaagcctgaa cctcataaac
    20941 tggctcgaga taacaaccaa atgcataaaa ttggaaaaat ggcagaagcg tttcgcatgc
    21001 gtttttgaat ttatattatg aataacatac aaataagaga ttatcagcct ggcgattttc
    21061 agcaactatg cgctattttc attagagcgg ttacgatgac cgtcagtcag cattattcac
    21121 tacaacaaat tgccgcctgg gcgcagattg acgaatctcg ctggaaggag aaactcgcga
    21181 aatcacaagt gcgggttgca gttattaatg caaaactggt cggctttatt acctgcgttg
    21241 aacactatat cgatatgtta tttgttgacc ctgaatacac ccgccgtggg gttgccagcg
    21301 ctttgttaaa accttttatt aagtctgaat ccgaacttac ggtggacgca agcataaccg
    21361 caaaaccctt ttttgaacgt tatggttttc agacagttaa gcagcagcgc gttgaatgcc
    21421 ggggagcgtg gtttattaat ttttatatgc gttataaacc gcaacattaa atccagcttg
    21481 caatgaaaat aacgcccgcc tagtatgtgc gcgttttctt attccacaaa ctgcaaggag
    21541 gtaaatcatg acaaatcctt tatcaatgac tctttgcaga cctttccagg attaattctt
    21601 tttttcttgc cctggattcg tctgccattt cctgattttt taattgttac tctcaataat
    21661 tcaagtcgca gcacatgcaa cttgaagtat gacgagtcta tttacggaat ggtatatggg
    21721 caattatatt cgtcccttat ccgatgcggt atttaccatc gcatctgatg acctgtggat
    21781 cgagagttta gcgatccaac aattacacac cacggcaaat ttacccaaca tgcagcgcgt
    21841 agtggggatg ccagatttac accccggacg cggctacccg attggcgcag cgttcttctc
    21901 cgcaggccgt ttttacccag cactggtcgg caatgatatc ggctgtggca tggcgctatg
    21961 gcaaacagat attctcgctc gcaaatacaa cgccgataag tttgaaaagc gattatcagc
    22021 tttggatgac gttgctgaag aaagctggct ggaggaaaac ctgccgtcag catttgcaca
    22081 gcatccgtgg cgcagctcgc ttggctctat cggtggtggc aatcatttcg ctgaactgca
    22141 acaagttgat caaatcatca acgctgaact gtttgcactg gcaggtctgg atgcgcagca
    22201 tctgcaactg ctggttcata gcggctcgag ggggctaggg cagtctattt tacagcggca
    22261 tattgcctcg ttttcgcatc atggtttgcc tgaaggcagt gacgacgcgc tggcgtttgc
    22321 gcgttttaat cgccacctga tcgctttgcg cataatgcaa caggttaagg ccaccggaag
    22381 tccggttctg gatgtggcgc ataactttgt tagcgcgtgt cgaatcggtg atcaacaagg
    22441 ggtcttacat cgtaaaggtg ccacgcctga tgactgtggt ctggtggtta ttcccggttc
    22501 acgcggtgat tactccaggc tggttcagcc cgtcaggagt gaggaaacat tgcattcgct
    22561 ggcgcatggg gctgggcgta aatgggggcg caccgagtgt aaagggcgtc tggcagcgaa
    22621 atacacagcg acgcaactct cgcgtactga acttggcagt cgggtaattt gtcgcgataa
    22681 acaactcatc ttcgaagaag cgccacaagc ctataaatcg gctgaaagtg tggtgcaatg
    22741 tcagggcagg ccggattaat tattcctgtc gcgcggctgc gtccggtgct aacgctcaaa
    22801 aacagtggag ggaaaaaagg atgatcttgc tataactctc ctcagcgcag gggccggaag
    22861 aatgttgtct cgcagtgaaa aaggcgctgg acaggctaat taaagaagct gcccgacaag
    22921 acgtcgcggt aacggtgctg gaaacagaaa caggccgcta ctctgacacg ctgcgttcgg
    22981 cgctgatttc tctggatggc gacaacgcat gggcgttaag cgaaagctgg tgtggcacta
    23041 ttcagtggat ttgcccgagc ccgtatcgcc cacatcacgg gcgtaaaaac tggtttctgg
    23101 gcattgggcg ttttaccgct gatgagcagg agcaatccga tgcgatccgg tatgagacgc
    23161 tgcgttcgtc ggggccgggc ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca
    23221 cgcatttggc atccggtatt agcgtgaagg ttcagtcaga gcgcagccag catgctaaca
    23281 aacggctggc gcgattgctg attgcctgga agctggatca gcagcaatag gaaaatagcg
    23341 cggtgctgaa atcacagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgcgac
    23401 gaacgtttac agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg
    23461 attaaacgcc atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt
    23521 cagtcagcag tgtccagtaa tgacctacgc tttcgatgtg aacttgctca tccggagagt
    23581 gtggaccggt gatagttggc ccgatagaaa ccatgtccat ttccggatac ggttttttga
    23641 acagaccaca ttccaggccc gcgtggataa tctggatgtt cggcgtcttg ttgaacaggc
    23701 gctgataggt ttcacgtacc agatgcatca ccggagaatt agcgtccggc tgccagccag
    23761 gatatgcgcc tttcgcttcg gttttcgcgc cagccagttt acccagcgaa tccagcatgc
    23821 tcaccacgta gtctttaccg ctgtcgatta gtgaacggat caggcagtga atttctacgt
    23881 tattgtcagt catggtcacc acaccgacgt tcagggaggt ttcgaccaca ccttttgcca
    23941 cgtcggaatt gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc
    24001 gagatttcgc aatcagggca gctttatcgt tcgctacaga gtccagcaac aaggccagat
    24061 ttttctcttt ctctgccagc tcgtttttca ggatctcctg ataggtattc accagagatt
    24121 tcagtgcgtc gactttatca gctgcgacag caatggtcgc aaaggcttca cgcgggatgg
    24181 cgttacgcag tgtgccgccg ttgaaatcga taaggcgcag gtccagttct tctgcatgac
    24241 ccgccaggaa gcgcaccagc agtttgttgg cattacccag gccaacgtgg atttcaccgc
    24301 cggagtgacc gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa
    24361 ccgcttcacg atctaaatgc aggttggagg tgaagtcgat accccccgca caacccatgt
    24421 agatttcacc ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc
    24481 tctgtaagcc gaacgcaccg tccataccgg cttcttcggt catggtcagc agcacttcca
    24541 gcgggccgtg aaccacgttt tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc
    24601 cgttatccgc acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct
    24661 ggataggatc tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga
    24721 ggtgggcctg taagacgacc ggtttacgat tttccatacc tgcggtagca ggttgacgaa
    24781 tcaggatatt acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa
    24841 caatgtattc agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg
    24901 caaaaatatc ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc
    24961 ttgtcgatca cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac
    25021 acaagacagg cttgcgagat atgtattgag aataccactt tatcccgcgt cagggagagg
    25081 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
    25141 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
    25201 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
    25261 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
    25321 tggcggtctg gtaccggggg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
    25381 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
    25441 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
    25501 tgttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
    25561 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggatcgaaca
    25621 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
    25681 cctggcattg ccgggcgttg ttctttttaa cttcaggcag gttacaatag tttccagtaa
    25741 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
    25801 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
    25861 ctgtgcagtc ggctcttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
    25921 tgatgtggat ctggcgcggt attgacccac gtgaaatcct cgacgtccag gcacgtattg
    25981 tgatgagcga tgctgaacgt accgacgatg atttatacga taccgtgatt ggctaccgtg
    26041 gtggcaactg gatttatgag tgggccaccc aggcgatggt gtggcaacaa aaagcctgta
    26101 cggaagaaga tccgcaactc agtggtcgac actggctgca tgcggctacg ttgtacaaca
    26161 ttgccgccta tcctcatctg aaaggagatg acctggccga gcaagcgcag gctttatcaa
    26221 accgcgccta tgaagaggcc gcccagcgtc tgccgggcac gatgcggcag atggagttta
    26281 ccgtacccgg cggtgcgccc atcaccggct ttttgcatat gccgaaaggc gatggcccgt
    26341 tcccgacagt attaatgtgt ggtggtctgg atgcgatgca gacggactat tacagcctgt
    26401 atgaacgtta ttttgcgccg cgcggcattg cgatgctgac tattgatatg ccgtcggtgg
    26461 gcttttcttc aaaatggaag ctcacccagg actccagcct gttgcatcag cacgtcttaa
    26521 aggcgctgcc taacgtaccg tgggtggatc acactcgcgt cgcggccttt ggtttccgtt
    26581 tcggcgctaa cgttgccgtg cgtctggcat accttgaatc gccgcgtctg aaagcggttg
    26641 cctgtcttgg tccggtagtt cataccctgt tgagtgattt taagtgccag caacaggtgc
    26701 cggaaatgta tcttgacgtt ctggcgagtc gtttggggat gcatgatgct tccgacgaag
    26761 cgttacgcgt ggagctgaat cgctattcat taaaagtgca aggattgctg gggcgtcgct
    26821 gcccaacgcc aatgttatca ggctactgga agaacgatcc gttcagcccg gaagaggact
    26881 cacgcttaat cacctcatca tctgctgacg gtaaattatt agagatccca tttaacccgg
    26941 tgtatcggaa ttttgacaaa ggtcttcagg aaatcaccgg ctggatcgaa aaacgcttgt
    27001 gttaaaaatt tgctaaattt tgccaatttg gtaaaacagt tgcatcacaa caggagatag
    27061 caatgacgtt accgagtgga cacccgaaga gcagattgat caaaaaattt accgcactag
    27121 gcccgtatat tcgtgaaggt aagtgcgaag ataatcgatt ctttttcgat tgtctggctg
    27181 tatgcgtcaa cgtgaaaccg gcaccggaag tgcgtgaatt ctggggctgg tggatggagc
    27241 tggaagcgca ggaatcccgt tttacctaca gctaccagtt tggtctgttc gataaagcag
    27301 gcgactggaa gagtgttccg gtaaaagata ctgaagtggt tgaacgactg gagcacaccc
    27361 tgcgtgagtt tcacgagaag ctgcgtgaac tgctgacgac gctgaatctg aagctggaac
    27421 cggcgaatga ttttcgtgat gaaccggtga agttaacggc gtgagtgaaa tgtgccggat
    27481 gcatcacatc cggcaatatt cattaaaact gatacgtcat cccaaccgcg acagtatcat
    27541 cattattaat attcagctta ttatcgctat ccagttggtt gattttataa tcaacaaacg
    27601 ctgacatatt tttgttgaaa taatacgtag caccgacgtc gatataattg accagatctt
    27661 catcaccgat gccctcaata tctttccctt tcgataagac ataacccagc gatggacgca
    27721 gaccaaagtc aaactggtat tgagcgaccg cttcaaagtt ctgtgtctta ttggcaaagc
    27781 cgccagttat tggcgtcatt ttgcgtgttt cagaatagaa agttgccaga taaatattat
    27841 tggcatcgta tttcagacct gttgcccatg cttctgcacg cttgcctgtg ccacggcttt
    27901 gcaggttctg ctcgttggtg cgatctgagt tggtataggc cccactaatg gcgaaatcgc
    27961 tgccgccaaa gtcatatgtc aatgacgtgc cgaagccatc gccgttttgc tttttaacgt
    28021 cgcggttttc gtttttccct tgatattgca gggttaagtt caggccatcg ataacgccga
    28081 agaagtcggt gttccgatac gtcgccagac cgctggcgcg tttggtcata aagttgtcgg
    28141 tctgcgccga ggaatcgcca ccaaattccg ggaacatatc ggtccaggct tccacgtcat
    28201 acaacgcgcc caggttacga ccataatcga aagaacccaa atctttatat ttcaacccgg
    28261 caaaagcgag acgcgttttt tgctgtgcag tatcactctc cgctttattt ccggcaaact
    28321 ccgcttccca ccgaccataa ccagtcagtt gatcgttaat ttgagtttca cctttaaaac
    28381 caaaacggat ataactctgg tcgccatctt tactggcgtt atcactcata taatgcatgg
    28441 ctttaacttt gccatagaca tccagtttat taccgtcttt attatatatt tctgcagcct
    28501 gtacagatgc agatgccaca atgcccatca ccactaatgc cagagtgctc tttttcattt
    28561 tcattcctga ttttaattaa cgcgcgaata ttcagcggga gagccccgtt gaaaacagga
    28621 aagtttttaa cctgagattg ttaaagatat attacagatt aatgatattc tgaaaa
//
LOCUS       DAETQL010000051        28422 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.051, whole genome shotgun sequence.
ACCESSION   DAETQL010000051 DAETQL010000000
VERSION     DAETQL010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.051"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            563..1333
                     /locus_tag="K0W65_002404"
     CDS             563..1333
                     /locus_tag="K0W65_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBI3095637.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1324..1797
                     /locus_tag="K0W65_002405"
     CDS             1324..1797
                     /locus_tag="K0W65_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBI3095638.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1904..2443
                     /gene="dnaT"
                     /locus_tag="K0W65_002406"
     CDS             1904..2443
                     /gene="dnaT"
                     /locus_tag="K0W65_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBI3095639.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2446..3183
                     /gene="dnaC"
                     /locus_tag="K0W65_002407"
     CDS             2446..3183
                     /gene="dnaC"
                     /locus_tag="K0W65_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBI3095640.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3232..3726
                     /locus_tag="K0W65_002408"
     CDS             3232..3726
                     /locus_tag="K0W65_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBI3095641.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3966..6257
                     /gene="opgB"
                     /locus_tag="K0W65_002409"
     CDS             3966..6257
                     /gene="opgB"
                     /locus_tag="K0W65_002409"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBI3095642.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6395..7417)
                     /gene="lgoD"
                     /locus_tag="K0W65_002410"
                     /pseudo
     CDS             complement(6395..7417)
                     /gene="lgoD"
                     /locus_tag="K0W65_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7556..8470
                     /locus_tag="K0W65_002411"
     CDS             7556..8470
                     /locus_tag="K0W65_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBI3095643.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8685..10046
                     /locus_tag="K0W65_002412"
     CDS             8685..10046
                     /locus_tag="K0W65_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3095644.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="K0W65_002413"
     CDS             complement(10095..11759)
                     /gene="tsr"
                     /locus_tag="K0W65_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HBI3095645.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASPEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="K0W65_002414"
     CDS             complement(11878..12324)
                     /gene="hpaR"
                     /locus_tag="K0W65_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HBI3095646.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12596..13885
                     /locus_tag="K0W65_002415"
     CDS             12596..13885
                     /locus_tag="K0W65_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HBI3095647.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPSDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13882..15348
                     /gene="hpaE"
                     /locus_tag="K0W65_002416"
     CDS             13882..15348
                     /gene="hpaE"
                     /locus_tag="K0W65_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HBI3095648.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15350..16201
                     /gene="hpaD"
                     /locus_tag="K0W65_002417"
     CDS             15350..16201
                     /gene="hpaD"
                     /locus_tag="K0W65_002417"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HBI3095649.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16211..16591
                     /locus_tag="K0W65_002418"
     CDS             16211..16591
                     /locus_tag="K0W65_002418"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HBI3095650.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16659..17462
                     /gene="hpaH"
                     /locus_tag="K0W65_002419"
     CDS             16659..17462
                     /gene="hpaH"
                     /locus_tag="K0W65_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HBI3095651.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17473..18276
                     /gene="hpaI"
                     /locus_tag="K0W65_002420"
     CDS             17473..18276
                     /gene="hpaI"
                     /locus_tag="K0W65_002420"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HBI3095652.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18298..19653
                     /gene="hpaX"
                     /locus_tag="K0W65_002421"
     CDS             18298..19653
                     /gene="hpaX"
                     /locus_tag="K0W65_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HBI3095653.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19663..20553
                     /gene="hpaA"
                     /locus_tag="K0W65_002422"
     CDS             19663..20553
                     /gene="hpaA"
                     /locus_tag="K0W65_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HBI3095654.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20580..21517
                     /locus_tag="K0W65_002423"
                     /pseudo
     CDS             20580..21517
                     /locus_tag="K0W65_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21744..23302
                     /gene="hpaB"
                     /locus_tag="K0W65_002424"
                     /pseudo
     CDS             21744..23302
                     /gene="hpaB"
                     /locus_tag="K0W65_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23320..23832
                     /locus_tag="K0W65_002425"
     CDS             23320..23832
                     /locus_tag="K0W65_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HBI3095655.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24225..26375
                     /gene="btsT"
                     /locus_tag="K0W65_002426"
     CDS             24225..26375
                     /gene="btsT"
                     /locus_tag="K0W65_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBI3095656.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26425..26628
                     /locus_tag="K0W65_002427"
     CDS             26425..26628
                     /locus_tag="K0W65_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBI3095657.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26639..27595
                     /gene="yjiA"
                     /locus_tag="K0W65_002428"
     CDS             26639..27595
                     /gene="yjiA"
                     /locus_tag="K0W65_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBI3095658.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27664..>28422)
                     /locus_tag="K0W65_002429"
     CDS             complement(27664..>28422)
                     /locus_tag="K0W65_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HBI3095659.1"
                     /translation="NYTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMART
                     EDFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQ
                     HADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQ
                     ALVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSF
                     YLTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6955 a         7425 c         7127 g         6915 t
ORIGIN      
        1 ttaaatgttt ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc
       61 atatcagcat actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa
      121 atgataacct ggtgaaatag cctggagaca tttaaaatat ctacattaag ttgttagaaa
      181 tacttaatga aaaacagaaa tctcattcat ttaatttata attaaatgat atctcttccc
      241 gcagaatgcg ggaatcacac aatcaatatt tattcaataa aatctgatga ttttaacttg
      301 ttaacactaa aaaacgcgcc cagctatcta ttatcctgac ccgtaaaggc taaacaataa
      361 attcattata gtgataatat tcatgtgata gtttgtttgc ggcgagagat aattcgcttt
      421 ttatcaccga gcttgtccca ataaccagga ttaatcagcg caattttgat cttaacctac
      481 cgcaaaacaa ccgtgcttca tgatacaaat ggattctcat tatctgaaca atacgcagca
      541 cgtctatgac aaagggcgag ttatgcaaac tgagcaacag cgagccgtaa cgcggctttg
      601 tatccagtgt ggattatttc ttttgcaaca tggtgcggaa agcgcgttgg ttgatgagct
      661 ttcctcacga ctgggtcggg cgctgggaat ggacagcgtc gaaagttcta tctcttcgaa
      721 cgccatagtg ctgacaacta ttaaagatgg gcaatgcctg acatcgacac gtaaaaatca
      781 cgatcgcggc attaatatgc atgtggtgac tgaagtccag cacattgtga ttcttgcgga
      841 gcatcatctg ctggattaca aaggcgtaga gaaacgattt agccaaattc aaccattacg
      901 ttacccaaga tggctggtag ccttaatggt tggcctttct tgcgcctgtt tctgtaaact
      961 caataacggt ggctgggatg gtgccgtcat caccttcttt gccagtacga ccgcgatgta
     1021 tatccgccag ctgctggcac aacgtcatct tcatccacag atcaactttt gccttaccgc
     1081 tttcgccgcc accaccattt ccggattgct tttgcaactc cccactttca gcaatacccc
     1141 caccattgcg atggccgcca gcgttctgct gctagtgccg ggctttccgt tgattaatgc
     1201 cgtcgccgat atgtttaaag gccacatcaa taccggactg gcacgctggg cgatcgccag
     1261 tctgctgaca ctggctacct gcgtcggcgt agtgatggca ctgacgattt gggggctacg
     1321 cggatgggtg tgatcgaatt tctgttagcg ttggcgcagg atatgatcct cgccgccatt
     1381 cctgcggtcg gctttgcgat ggtgttcaac gttcccgtgc gggcgttacg ctggtgtgcg
     1441 ctgcttggct cgataggtca tggttcccga atgatcttga tgaccagcgg gttgaatatt
     1501 gagtggtcaa cctttatggc ttctatgctg gtcggtacca ttggtattca atggtcgcgc
     1561 tggtatctgg cgcatccgaa agtgtttacc gtggcggccg ttatccctat gttcccgggc
     1621 atatcggctt ataccgcaat gatttcgtcg gtaaaaatca gccagttagg ttacagcgaa
     1681 ccgttgatga ttaccctgtt aaccaacttt cttacagctt catcgattgt tggtgcgtta
     1741 tccatcggtc tttccattcc tggattatgg ttgtaccgca agcgccctcg cgtataaaat
     1801 tgcctcgctt aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa
     1861 gttatctccc ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt
     1921 gaccccggac gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa
     1981 agctgaaggc ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac
     2041 gcctgcacgc ctggctgaac tgctggcgct ggaagaaaag ctagcgcgtc cgggaagcga
     2101 tgtcgctctg gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtacccat
     2161 ggggaaattc gccatgtatc cggactggca acccgatgcc gattttatcc gcctggcggc
     2221 gctatggggc gtggcgctaa gagagccggt gaccaccgaa gaactggcct cattcattgc
     2281 ctactggcag gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg
     2341 cagcctgcaa atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt
     2401 cagtgaacct gacagccaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc
     2461 gacctgatgc aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg
     2521 ggtgaagagc tactggcctg gcagaaagaa caaggggcga tccgctccgc cgctctcgaa
     2581 cgtgaaaatc gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcgtccactg
     2641 catcagaact gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta
     2701 agcaaagcgc gccagtatgt cgaagagttt gacggcaaca tcgccagctt catcttttct
     2761 ggtaagccag gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta
     2821 cgcggtaaat ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc
     2881 ttcaggaata gcggtaccag cgaagaacaa ctgcttaacg atctgagcaa cgttgatctg
     2941 ctggtgatcg atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac
     3001 cagatcgtcg atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat
     3061 atggaagaga tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac
     3121 agtttatggg tgatcttcaa ctgggatagc taccgtagcc gggtaacggg taaagagtat
     3181 taatccccgt ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa
     3241 actattaaac atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg
     3301 catgctgcgt cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac
     3361 agcacgacac aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcgga
     3421 aaccaagcct taagcgcaga taacatgaac aatgccgcag gcattctgca atactgcgcg
     3481 aagcaaaagc tggcttcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag
     3541 ctgggcctga acagtgaaga gcaaaaagaa gacaccaact atctggacgg tattcagggt
     3601 ttgctgaaaa caaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg
     3661 gcagaaaagg tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt
     3721 tcctgaaccg cgaaatgtta cccggctgac gcggctctct cctgcccttt tccctgctcc
     3781 tggacggttt tccccctgtt acacgggaag cgaccgcgat caaaaaagtc tgtttataaa
     3841 ctaattccta acaacatcgc atttttttga cactagaatt gcagcaatat ggcgacgcca
     3901 ttacattgtg cttctcaaaa agcttaaacg ttggctaaac ggcccggttt agtgaggatc
     3961 gcccattgtc agaactactc tctttcgccc tttttctcgc ctctgtgctg atttacgcat
     4021 ggaaagcggg acgtaacacc tggtggtttg cagccacgtt aacggtgctg gggctatttg
     4081 tcgttttaaa tatcaccctg tttgccagcg actattttac tggcgatggt attaacgacg
     4141 cggttctcta taccttaacc aacagcctga ccggtgctgg cgtcagcaaa tacatcctgc
     4201 cgggtatcgg cattgtgctg gggctgacgg cggtgttcgg tgcgctgggc tggatcctgc
     4261 gccgtcgtcg tcatcatccg caccattttg gttacagcct gctggcgctc ttactggcgc
     4321 tgggttcggt ggacgccagc ccggcatttc gtcagataac ggaactggtg aaatcccagt
     4381 cacgcgatgg cgacccggac tttgcggctt attataaaga gccgtcgaaa actatccctg
     4441 acccgaaact caacctggtt tatatctacg gcgaaagtct cgagcggacc tattttgata
     4501 acgaagcttt cccggatctc acgcctgaac tgggcgcgtt gaaaaatgaa ggcctggatt
     4561 tcagccacac gcagcagctg ccaggaacgg attacacgat tgcgggcatg gtggcttctc
     4621 agtgcggcat accgctgttc gccccctttg aaggcaacgc ctccgcctct gtctccagct
     4681 tcttcccgca gaacatctgt ctgggcgata tcctgaaaaa ctcgggttat cagaactatt
     4741 tcgtgcaggg cgcaaatctg cgttttgccg gtaaagatgt gttcctgaaa tcgcacggct
     4801 tcgaccactt atacggctca gaagagctga aaagcgtggt agccgacccg cactatcgca
     4861 acgactgggg attctacgac gataccgttc tcgatgaagc gtggaaaaag tttgaagagc
     4921 tttcccgctc aggtcagcga ttctcactgt ttaccctgac agtcgatacc catcacccgg
     4981 atggttttat ctcgcgcacc tgtaaccgca aaaaatatga ttttgacggt aagccgaatc
     5041 agtcattcag cgcggtaagt tgcagccagg agaatatcgc gacgtttatc aacaaaatca
     5101 aagcgtcacc gtggtttaaa gatactgtca tcgtcgtctc ttctgaccat ttagcgatga
     5161 acaacacggc gtggaaatac ctcaataagc aggatcgcaa taacctgttt tttgtcattc
     5221 gtggcgacaa gccgcagcaa aagacgctgg cagtgaagcg taacacgatg gataacggcg
     5281 cgacggtgct ggacattctc ggtggcgata actatctcgg acttggtcgt agcagtttat
     5341 ccgggcagtc gatgtcggaa atcttcctca atatcaaaga gaaaacattg gcgtggaagc
     5401 cagatatcat ccgcctgtgg aaattcccta aagagatgaa agagttcacc atcgaccagc
     5461 agaaaaacat gattgccttc tcgggtagcc atttccgttt gccgctgctg ttgcgggttt
     5521 cagacaaacg cgtggaaccg ctgccggaaa gcgaatactc agcaccgctg cgtttccagc
     5581 tggccgattt cgctccacgc gacaatttcg tctgggttga cagttgctac aagatggcac
     5641 aactctgggc tccggaactg gcactttcca ccgactggtg tgtctcgcag gggcagcttg
     5701 gcggtcagca aattgttcag catgttgaca aaacaacatg gaagagcaag acggcattta
     5761 aagatacggt catcgacatg gcgcgttaca aaggcaatgt cgatacgctg aagattgttg
     5821 ataacgatat tcgctacaaa gccgacagtt tcatctttaa cgtcgccggt gcgccagaag
     5881 aggtgaaaca gtttagcggg atttcccgtc cggagtcgtg gggccgctgg tccaacgcgc
     5941 agctgggcga tgaagtaaaa atcgagtaca agcatccgct gccgaagaaa tttgacctgg
     6001 tgattaccgc caaagcatac ggcaataacg ccagccgtcc tattccggta cgcgtaggca
     6061 atgaagaaca aactcttgtg ctgggcaatg aagtgaccac caccacactg catttcgata
     6121 acccaaccga tgccgacaca ctggtaattg tgccgccgga acctgtctca accaacgaag
     6181 ggaatatcct cggacactcg ccgcgtaagc tcgggatcgg catggtggaa attaaagtgg
     6241 tagaacgtga agggtaattc ctgactgatg tgtaggtcgc atagggtgcg acctacacgc
     6301 cttctccggc ttacaacaca ttattaagaa acaaaaaaaa cagcgcccaa cacttccccg
     6361 gacgctgcat ggcaacttta ataatctcgc cttatcagaa agtaattaca cccttaatca
     6421 actcacgatt gttaatcaca tcgcgctcgt aggtttctgc cagcgtggcg aacggatagc
     6481 gatgggttaa catcatgtcg gcagtgattt tcccttccgc catcagtcga ccgactttag
     6541 cgaaatcttc cggcgtggcg ttgcggctgc ccatcatcgt cgtttctttt ttatggaatt
     6601 ccggatcgga gaactgcaac tcacctttaa acagaccgac aaataccacc gtgccgccgt
     6661 gacgaatcag attcacggta ttattcatcg catgttgatt acctgtcgcg tcgatcactt
     6721 tctgcgccag cgaaccacca aactgcgccc gcagctgcgc gtcaaaatct tcggctgacg
     6781 ggtccagcac gggcaactcc agacgcgttg ccacatgttc acggcgcgcc ggactggtat
     6841 ccgccaccac cacctgtgcg ccatcggctt tagcgattgc cgccgcgccc agaccgattg
     6901 gccccgcccc gaccaccagc acctgctcgc cgggagcaat ggctgcgcga cgcaccgcat
     6961 gagcgctaat agcgaaaggt tcaatcaatg cggccgcctg cgggtcaata ccgtctgcgg
     7021 gcaaaatgtt cgccaccggc acgctcaaat actcactaaa accgccatcc tgatgcacgc
     7081 caatgactga aattttttca cagcagttgg tacgcccgct tttacacgcc gggcattgct
     7141 gacaggcaac ataagggatc acagcaactt gctgaccatt tttaagatca gcaatatttt
     7201 tacccagccc aacaatctcc ccacatattt catggcctaa aacccgtgga taactaaaaa
     7261 atggttgatt tccaccctag gcatgaatat cggtaccgca aatcccgaca gacttaattt
     7321 ttattaatgc ttcattgtca cccggaatag gtatctcgcg ttgtttccag actaattctt
     7381 tcggctgctg gcaaattaaa acattcatcg tagacatgat tcggctatcc attttttgtt
     7441 gccttagata tcaatgaaaa tgaaaaatgg cagcggaacg gaaaatctgt tttgtgaaaa
     7501 cacacgcata aaaatgtttt aaatcgggtt ttaatggact cacaaggagg aaaatatgag
     7561 tcgttcacaa aatttacgcc acaatgtgat taaccaggtc attgatgata tggcgcgggg
     7621 tcatatccct tccccacttc catcgcaaag tgcgctggcg gaaatgtaca acatcagccg
     7681 cactacagtg cgtcatattc tcagccaatt acgcgaatgc ggcgtcttaa cgcaggttgg
     7741 aaacgactat gttattgcgc gtaaacctga ccatgatgac ggtttcgcct gtactaccgc
     7801 ttcaatgagt gaacaaaaca aagtgtttga gcaggctttt ttcacgatga taaaccagcg
     7861 tcagttacgc cctagggaaa ctttttctga actgcaactg gctcgggccg cgggtgtcag
     7921 cccggtggtt gtaagagaat atcttttaaa attcggccgt tataatctta ttcaaagcga
     7981 aaagcgcggc cagtggagca tgaagcaatt cgatcaatcc tacgccgagc agctgtttga
     8041 gctacgcgaa atgctcgaaa cacattcact tcagcacttt ctcaacctgc ctgatcatga
     8101 tccccgctgg ctgcaggcga aaaccatgct agaacgtcat cggctactgc gtgacaacat
     8161 cggcaatagc tttcgcatgt tctcgcaact tgacagagat tttcactcct tactgctttc
     8221 agctgccgac aatatctttt ttgatcaatc gcttgagatc atctcagtca tctttcattt
     8281 tcactatcaa tgggacgaaa gcgatcttaa acagcgcaat atcattgccg tcgatgaaca
     8341 tatgaccatt ctcagcgctc tgatttgccg cagtgacctt gatgccaccc ttgcgctccg
     8401 taaccatttg aattcagcta aacaatcaat gattcgatcc attaatgaga acacgcgtta
     8461 tgctcattaa gtacggatta aaaacagcaa actattagcg ggtgcacggt tttatcaaaa
     8521 gataaatacc gggaaattcc cgcttaccta tgctcacaat cagtcgacta aaaatgtttt
     8581 tgtgagcggt agtaaagtcc taaaacttta acctgccgtt aatgtcaacc ttgcgtaatg
     8641 cacaattacg taaacaatta aaattataaa tacaggagtc cggcgtggaa aaagaaaata
     8701 tcaccctcga tccgcgttct tcatttactc catcttcgtc ggcagatatt cccgtgccac
     8761 cagatggatt agttcaacgc agtacccgaa ttaaacgcat tcaaaccacc gccatgttgt
     8821 tattattttt tgcggcggta atcaattatc tcgaccgcag ttcgctgtcg gtagcaaatt
     8881 taacgattcg tgaagaattg ggattaagtg ccaccgaaat cggcgctttg ctctccgtgt
     8941 tttcactcgc ttacggcatt gcgcaacttc cttgcggccc actattggat cgtaaaggcc
     9001 cacgcctgat gctgggactg gggatgttct tctggtcact gttccaggcc atgtctggca
     9061 tggtgcacag ctttacgcag ttcgtgttgg tgcgtatcgg tatggggatt ggtgaagcgc
     9121 cgatgaaccc atgcggtgta aaagtcatta acgactggtt caacatcaaa gagcgcggac
     9181 gcccgatggg cttcttcaac gcagcttcta ccattggcgt tgctgtaagc ccaccgattc
     9241 tggcggcaat gatgctggtg atgggctggc gcgggatgtt tattaccatt ggtgtactgg
     9301 ggatttttct cgccatcggc tggtatatgc tctatcgcaa ccgcgagcac gtagaactga
     9361 ctgccgttga acaagcttat ctcaatgcag gtagcgtcaa tgcccgccga gatccgctca
     9421 attttgccga atggcgcagc ctgttccgta accgcacaat gtggggaatg atgctcggat
     9481 tcagtggcat caactacact gcgtggctgt atctggcctg gcttcctggt tacctgcaaa
     9541 cagcctataa cctggattta aaaagcacag ggttgatggc ggctatccct ttcctgtttg
     9601 gggctgccgg gatgctggtc aacggttacg ttactgactg gctggtcaaa gggggaatgg
     9661 ctccgattaa aagccgtaag atctgcatta ttaccgggat gttctgttct gccgccttta
     9721 cgctgatagt accacaagcg acaacatcca tgatggcggt tctgctgatt ggcatggcac
     9781 tgttctgtat tcactttgcc ggaacatcct gctggggctt gatccacgtc gcagttgctt
     9841 ctcgcatgac tgcgtcggtg ggcagtatcc agaactttgc cagcttcatc tgcgcctctt
     9901 tcgcgccgat cattactggt tttattgttg atacaaccca ctcattccgt ctggcactaa
     9961 tcatttgcgg ttgcgtcacc gcagcgggag cactggcgta catcttcctg gttcgccagc
    10021 cgatcaacga cccgcgtaaa gattaaactc cctatgcccg ataacatttt gcttatcggg
    10081 catttttatg gcgattaaaa cgtttcccag ttctccccgc tatctgccac tgccatttta
    10141 cgcggcgtag ctggcgtcac ggtttttacc acagccgatg tttcacgctg ctgttgctgc
    10201 tgttgctgaa tccggaacac tgccacagct tcggtcaggc gactggcctg ctcttccagc
    10261 gcggcggcgg cagcggcaga ttcttccacc agcgcggcgt tctgttgagt tacccggtcc
    10321 atctcagcaa ccgctaagcc aacctgatcg ataccacggc tctgctcatc agaagcagaa
    10381 gctatttcgc ccataatgtc cgtcacgcgg gtcacagcac tgacaatctc cgccattgtt
    10441 tccccggcgc tttcgaccag cgtagagcca acatccactt tccccaccga gtcttcaatc
    10501 aggcttttaa tttcacgaac cgcctgggcg ctacgctggg ccagattacg cacttctccc
    10561 gcaaccaccg caaaaccacg tccttgctca cccgcacgcg ctgcttcaac cgccgcgttc
    10621 aaagccagaa tattggtctg gaaggtaatg ccgtcaatta cgctgataat atcggcgatt
    10681 ttctgcgaac tggtggagat atcgcgcata gtctgcacca cgttatctac cactttgccg
    10741 ccgcgttgcg ccgtttcaga agcacttaat gccagatggc tggcctggcg cgcattctcg
    10801 gcgttctgtt tcaccgttgc ggtcagttgc tccatgctgg ctgccgtctc ttccggcgaa
    10861 gcggcctgtt gctcggtgcg cgaagagaga tcattattgc cggtagcgat ttcgctggca
    10921 ccgctataga tggcattggc cccgttgcgc acatcaccga cggtacgcat cagctctccc
    10981 tgcatatggc gcaaactctc tgccagttgc cccatctcat tagagccatc cacctcaatc
    11041 ggtttcacca gatcgccgcc tgcaatatga cgaatgctgt caatcaggcg attcattggc
    11101 gctaccagcg aggctttaat accgaaccag acggcgaaga tgaccgccag tacgacgatc
    11161 atcacgccca ccagaatcca catcgcctgg ctgtaggagg cattgttatc gctgacggcg
    11221 atatcataga gccgatcgtt ttgctccatg taagccacat actgcttctc gaaaccgtcc
    11281 tgatatccct gggtcggctg atcaaagaac tcgttgattt tgcctgcacc taacagttgg
    11341 atcagctccg ccagcgcatt gtgataaata tcgtaattac gtttgatctc tgccgctgcg
    11401 gcggtgctct gacgcgggtc acgcggcaac gcttcgtaat ccgcccagtt tttttccgcc
    11461 tgtttcagcg aaatactggc actctgcatc agctcagcaa cggttgaacc gctaccaata
    11521 ttattctgat ccatcatgta gcggataccc gcgcggttga gggtgttacg cgtctgcaac
    11581 aacgcgaccc agctgccatt cagcgtgggt tgctgctggc gaatggtttg taaaacagtg
    11641 aaattttctt tgtcattctt taaggcatta aaaaacagac cgcctgatgt cagttgtaaa
    11701 aggccaaaaa cggccaaaac cagcagtaag ctggtcacga tcttgatacg ttttaacatg
    11761 gtttctcttt cctgtggacc gatgcaagat tttcggcctg gaaagcaaaa actttatgaa
    11821 aaggtgtaac ccgccatcca tggaaaaacc aggcgctggt tatcagatac taaaaagtta
    11881 ttcattatcg cccgggatgt cttcttgtcg ggaattgccc agagcaataa attcctctaa
    11941 cagatgggtt aactgttgca ttttctcggc agtaaactga gcttcaatct gccgataggc
    12001 ttcttcaatc tgtgtctgag cgcggttata cagcgcctgc ccctctttgg tcagcgagat
    12061 atagagcttc cgctgatcat tgatcggctt taatcgcaac actaaaccgt cgcgttccat
    12121 ccgcgtgagg atcccggtca ggcttgggcg caaaatgcag gcgcgatacg ccagatcgtg
    12181 aaaatccatt gatgggcttt ccgccagaat acgcacgatg cgccactgtt gctcagtcaa
    12241 attatgccgt ttaacgattg ggcgaaaata actcatcgcc gcttctcgcg cctggagcaa
    12301 cgcaatggtt agtgagtcgt gcattatctt tcccctgaaa aattcaaaat cattaatata
    12361 gaaacaatta atcatcacgt taccgggact gagctgcccc cgcaattctt cattttatca
    12421 aaattgatga tattttatta acttactgat atttaatatt ttattatctt taaatgtaaa
    12481 tagtttgtta attagatcac atttatatca cttacttttg ccaaactacg caatcgggaa
    12541 taaaagtaat cattaacata ttaatgatta agatgatcga atccgaggag tggtaatgaa
    12601 aggcactatc ttcgccgtag cgttgaacca tcgcagccaa cttgatgcct ggcaggaagc
    12661 gttccagcaa tctccctaca aagccccgcc taaaactgcg gtctggttta ttaaaccgcg
    12721 caatacggtg attggttgcg gtgaaccgat tccctttcca cagggtgaaa aagtactgag
    12781 cggtgcgact gttgcgctga ttgtggggaa aacggcgacg aaagtacgtg aagaagatgc
    12841 ggcagagtac atcgccggat atgcgctgga taacgacgtc agcctgccgg aagagagctt
    12901 ttaccgtccg gcaatcaaag caaaatgtcg tgatggattc tgccccattg gcgaaaccgt
    12961 ggctctcagc aatgtcgata atctgactat ctataccgag atcaacgggc gtcctgccga
    13021 tcactggaat accgccgatt tacaacgtaa cgccgcacag ctgctgagcg ccctgagcga
    13081 atttgccacg ctgaatccaa gcgatgctat tctgctcggc acgccacagg cgcgcgtgga
    13141 aatacagcca agcgatcgcg tgcgtgttct cgcagaaggt ttcccgccgc tggaaaatcc
    13201 ggtagtggac gaacgtgaag tgaccacgcg caagagcttc ccaacgctgc cacacccgca
    13261 cggtacgctg tttgccctcg gcctgaacta cgccgaccac gccagcgaac tggaatttaa
    13321 gccaccggaa gagccgctgg tgttcctgaa agcgccgaat accctcactg gcgacaacca
    13381 gacctccgtg cgcccgaaca atattgaata catgcactac gaagcggagc tggtggtagt
    13441 tattggcaag caggcgcgta acgtcagcga agccgatgcc atggattatg tcgcgggcta
    13501 caccgtgtgt aacgactacg ccattcgcga ctatctggaa aactactacc gccctaacct
    13561 gcgggtcaaa agccgcgacg gactgacgcc gatgctttca accatcgtgc cgaaagaggc
    13621 gatcccggac ccgcataatc tgacccttcg caccttcgtc aacggcgagt tacgccagca
    13681 aggcaccacc gccgatctga tcttcagcgt gcccttcctg atcgcctact taagcgaatt
    13741 tatgaccctg aatccgggcg acatgatcgc caccggcaca ccaaaaggct tatctgacgt
    13801 ggtgcctggc gatgaagtag tggtggaagt agaaggcgtg ggccgcctgg tgaaccgaat
    13861 tgtgagtgag gaaacagcga aatgaaaaaa gtaaatcatt ggatcaacgg caaaaatgtt
    13921 gcaggtaacg actacttcca gactaccaat ccggcaacgg gtgaagtgct ggcggatgtg
    13981 gcctctggcg gtgaagcgga gatcaatcag gcggtagcgg cagcgaaaga ggcgttcccg
    14041 aaatgggctg atctgccgat gaaagagcgt gcgcgcctga tgcgccgtct gggcgatctg
    14101 atcgaccaga acgtgcctga gatcgccgcg atggaaaccg cggacacggg cctgccgatc
    14161 catcagacca aaaatgtgtt gatcccacgc gcttctcaca actttgaatt tttcgcggaa
    14221 gtctgccagc agatgaacgg caagacctat ccggttgacg acaagatgct caactacacg
    14281 ctggtgcaac cggtaggcgt ttgtgcgctg gcgtcgccgt ggaacgtgcc gtttatgacc
    14341 gccacctgga aggtcgcgcc gtgtctggcg ctgggcaata ccgcggtgct gaagatgtcg
    14401 gaactctccc cgctgaccgc cgaccgcctg ggtgagctgg cgctggaagc tggtattccg
    14461 gcgggcgtgc taaacgtggt acagggctac ggcgcaaccg caggcgatgc gctggtccgt
    14521 catcatgacg tgcgtgccgt gtcgttcacc ggcggtaccg ccaccgggcg caacatcatg
    14581 aaaaacgccg ggctgaaaaa atactccatg gagctgggcg gtaaatcacc ggtgctgatt
    14641 tttgaagatg ccgatattga gcgtgcgctg gacgccgccc tgttcaccat cttctcaatc
    14701 aacggcgaac gctgcaccgc cggttcgcgc atctttattc agcagagcat ctatccagaa
    14761 ttcgttaaac gctttgccga acgcgccaac cgtctgcgcg tgggcgatcc gaacgatccg
    14821 aatacccagg ttagggcgct tatcagtcag caacactggg aaaaagtctc cggctatatc
    14881 cgtctcggca ttgaagaagg cgcaaccctg ttggcgggcg gcccggataa accgtccgac
    14941 ctgcctgcgc acctgaaagg cggcaacttc ctgcgcccaa cggtgctggc ggacgtagat
    15001 aaccgtatgc gcgttgccca ggaagagatt ttcgggccgg tcgcctgcct gctgccgttt
    15061 aaagacgaag ccgaaggctt acgcctggca aacgacgtgg agtacggcct cgcgtcgtac
    15121 atctggacac aggatgtcag caaagtgtta cgcctggcgc gtggcattga agcaggcatg
    15181 gtgttcgtca acacccagaa cgtgcgcgac ctgcgccagc catttggcgg cgtaaaagcc
    15241 tccggcaccg ggcgtgaagg cggtgagtac agcttcgaag tgttcgcgga aatgaagaac
    15301 gtctgcattt ccatgggcga ccatccaatt ccgaaatggg gagtctgata tgggtaagtt
    15361 agcgttagcc gccaagatca cgcacgtacc gtcgatgtat ctctctgaac taccaggaaa
    15421 aaaccacggt tgtcgccagg gtgcgatcga cgggcataaa gagatcagca agcgttgccg
    15481 ggaaatgggc gtcgatacca ttatcgtttt cgatactcac tggctggtca acagtgctta
    15541 tcacatcaac tgtgcagacc attttgaagg cgtctacacc agcaacgagc tgccgcattt
    15601 tattcgcgac atgacctaca actacgaggg caacccggag ttggggcagc taattgccga
    15661 ggaagcctta aagctcggcg tgcgggcaaa agcgcacaat attcccagcc tgaaactgga
    15721 atacggcacg ctggtaccga tgcgctacat gaatgaagac aagcacttca aagtggtctc
    15781 catttcggct ttctgcacgg ttcacgattt tgccgacagc cgcaagctgg gcgaagcgat
    15841 tctgaaagcg atcgaacagt acgacggcac cgtggcggtc cttgccagcg gttcgttatc
    15901 gcaccgcttt attgacgatc agcgtgcgga agaagggatg aacagctaca cccgcgagtt
    15961 cgaccgccag atggacgagc gtgtggtgaa gctgtggcgc gaaggccagt tcaaagagtt
    16021 ttgcaatatg ctgccggagt acgccgacta ctgctacggc gaaggcaata tgcacgacac
    16081 ggtgatgctg ctggggatgc tcggctggga taaatacgac ggcaaggtgg agtttattac
    16141 cgagctgttc ccaagctctg gcaccggtca ggttaacgct gttttcccgc tgcccgcgta
    16201 aggacgtttt atgccgcact ttatcgtgga atgtactgaa aatattcgtg aagaagcccg
    16261 cttacctgag ctgtttgcca gtgttaacac tgcactggcg gcaacgggga tttttccgct
    16321 tgggggaata cgtagccggg cgcactggat cgatacctgg caaatggctg atggcaagca
    16381 cgattacgcc tttgtgcata tgacgctaaa aatcggcagc ggacgcagtc tggaaagccg
    16441 tcaggaagtg ggcgaaatgc tgtttgatct gattaaaacg cacttcgcct cactgatgga
    16501 gagccgttac ctagcgcttt catttgagat tgcagagctg cacccgacgt tgaatttcaa
    16561 acagaacaac gtacacgcgt tgtttaaata aatttgcacc agcttccagg ccggatgata
    16621 aggcgctgcc gcatccggca atgccacagg atatcgctat gttcgataaa cacacccaca
    16681 ccctgatcgc ccagcgtctg gatcaggcag aaaaacagcg cgaacagatc cgcgcgatct
    16741 cgctggatta cccggagatc accatcgaag acgcctacgc cgtgcagcgc gaatgggttc
    16801 gcctgaaaat cgccgaaagt cgcacgctga aaggccacaa aatcggtctg acctcgaaag
    16861 cgatgcaggc aagctcgcag atcagcgaac cggattacgg tgcactgctg gacgacatgt
    16921 tcttccacga tggcagcgat atcccgaccg atcgctttat cgtgccgcgc attgaagtgg
    16981 agctggcttt tgtgctggca aaaccgctgc gtgggccaaa ctgcacgctg ttcgacgttt
    17041 acaacgccac ggactatgtg atcccggcgc tggagctgat cgacgctcgc tgccacaaca
    17101 tcgatccgga aacccagcgc ccgcgcaaag tattcgacac catttccgat aacgccgcca
    17161 acgccggagt gatcctcgga ggtcgtccga tcaaaccaga cgaactggat ctgcgttgga
    17221 tctccgccct gatgtatcgc aatggcgtga ttgaagaaac cggcgtcgcc gctggtgtgc
    17281 tgaatcatcc ggcaaacggc gtggcctggc tggcgaacaa actcgcaccg tatgacgtac
    17341 aactggaaac cgggcaaatc attctcggtg gttcgttcac cctcccggtt ccggcgcgta
    17401 agggcgacac cttccacgtc gattacggca acatgggctc cattagctgc cgctttgttt
    17461 aaggagagaa cgatggaaaa cagttttaaa gcggcgctga aagcaggccg tccgcagatt
    17521 ggattatggc tggggctgag cagcagctac agcgcggagt tactggccgg accaggattc
    17581 gactggttat tgatcgacgg tgagcacgcg ccgaataacg tgcaaaccgt gctcacccaa
    17641 ctacaggcga ttgcgcccta ccccagccag ccagtggtac gcccatcgtg gaacgatccg
    17701 gtgcaaatca aacaactgct ggacgtaggc acacaaacct tactggtgcc gatggtacaa
    17761 aacgccgacg aagcccgtaa agcggtacgc gccacccgtt atccccccgc cggtattcgc
    17821 ggtgtgggca gtgcgctggc tcgcgcctcg cgctggaatc gcattcctga ttacctgcaa
    17881 aaagccaacg atcaaatgtg cgtgctggtg cagatcgaaa cgcgtgaggc aatgaagaac
    17941 ttaccgcaga ttctggacgt ggaaggggtc gacggcgtgt ttatcggccc ggcggatctg
    18001 agcgccgata tgggttatgc cggtaatccg cagcacccgg aagtacaggc cgccattgag
    18061 caggcgatcg tgcagatccg cgaagcgggc aaagcgccgg ggatcctgat cgccaatgag
    18121 caactggcaa aacgctatct ggaactgggc gcgctgtttg tcgccgtcgg cgttgacacc
    18181 accctgctcg cccgcgccgc cgaagcgctg gcagcacggt ttggtaatag ttcttctatt
    18241 tcgatggcga agcaaaacaa caactctgtc tactaaacgt acgatgtgga gcgcatcatg
    18301 acgacctcta ccctgcaata taaagataat aaaactgttg aaattgaaca tcgtgtcatt
    18361 aataaattat ttcgtcgcct aatcattttt ctttttattc tgtttgtttt ttcgtttctt
    18421 gatcgcatca atataggatt tgccggactg acaatgggca aggatcttgg cctgacgtcc
    18481 acaatgtttg gcctcgccgc aacactcttt tatgtcacgt acgtcatgtg cgggatcccc
    18541 agtaatatga tgttagcgaa aattggcgct cgtcgatgga tcgctgggat catggtggtc
    18601 tggggaattg cttccacctg cacaatgttt gcaacaagcc ccgaaacact ctatctactg
    18661 cgtatgctgg ttggtattgc tgaggcgggt tttttacctg gtattttggt ttatctgacc
    18721 tggtggttcc cggcatatca tcgggccaga gccaatgcct tatttatgat agcgatgcca
    18781 gtcactatga tgctgggatt gatcctttca ggctacattc tggccttgga tggactgtgg
    18841 aatctaaaag gctggcaatg gctctttttg ctggaaggtc tgccatcggt cattcttggt
    18901 gtcgtcacct ggttttatct caacgacaca ccagaccagg cgacctggct tgatgaaaac
    18961 gaaaaacagg ctttaaaaac gatgattctc cgtgaacaag agagtgcatt tgcccatgca
    19021 tctgttccac gctcaacctt acgtgaagtc ctgaccccag ccatcatgct atatacactt
    19081 gcttattttt gcctgactaa tactctgagt gcgattaata tctggacgcc gcaaatccta
    19141 cagagtttca atactggcag tagcaacatc atgattggtt tgctggcggc catcccccaa
    19201 ttctgcacaa tttttggcat gatctggtgg agtcgtcgtt ccgaccgtct taaagaacgc
    19261 aaaaagcata cgattcttcc ctacctgttt gccgctgccg gatggttact cgcttctgcg
    19321 acagatcaca gcctcattca actgagcagc atcattatgg cctcaatggg atcatttacc
    19381 gcgatgacta tcttttggac aacaccggat caagttataa gcttgcaatc gcgtgccgtt
    19441 gcgatggctg tcatcaatgc tgtcggcaat gtaggttcag cagtcagtcc attacttatc
    19501 ggtattttga aagatgcaac cggcagtttt agctcaggtt tgtggtttgt agcaggattg
    19561 ctggtactgg gcgcgttagt catgacccga atccctatgg ggagtcggga acactctctt
    19621 tcatctgaac ttatcgcacc aaaaccgcaa taaggagtca tgatgtccag tgaagctatc
    19681 tttgaacatg gttatgaaaa tattgatttg gtccgggaat atgatgcccg ctacgctggt
    19741 gaagatgtgc attatgaaac atttgcccgg ttagcgaatt tctttggacg cgatatgcgc
    19801 cctcactggc acgatagata ttttcaactc cattatctgg tcactggacg cattacttta
    19861 caactggatg agcacttcta ttctgtgcgc gcaccgcttt ttgtactaac accaccatcg
    19921 gtgccacata ctttctttac tgatcctgat agcgatgggc atgttctgac cgtacgtcag
    19981 gagttaattt ggcccctggt agaaaagctc tggcctggta gtggagatgc cgcaatgcta
    20041 caaggtatct gtctgtcact ggagggaatg gaacatacac tggcggcatt aaatcattat
    20101 tggccgataa tatccctgga gtttcaacaa aataagcaag gacgggaaat tctattgcaa
    20161 tcactggcgc aaagtatttt tacaatccta ctgcgtgaag caccaccaaa tgatatttca
    20221 acatgcagcg tgcgcggcga gatgtgttta ttccagaagt ttaatcggct gattgacgaa
    20281 aattatcgcc aacatttaat ggtccctgaa tatgcaacaa tgctcggtat gagtgaatca
    20341 cggcttaccg agctgtgccg acgttttgcc aatcaatcgc cgaagagatt gattttcgaa
    20401 cgagtgagcc gggaagccag acggcttctg ctctatagcg agcaaagcat caaccagata
    20461 gcccttgatc ttggctacaa agatcccgcc tatttcgccc gttttttcaa ccgaatgatg
    20521 ggttgctcac caacacaatt ccgtggtcgc tgaccattta tcaacctgag agactcgaca
    20581 tgaaaatatc tttacgctca ctatttgtgg cgtgcctgat tgcgccagtg gcattcacct
    20641 acgcctccga accgcaataa atgaaaattg tactacttgg aacgaaaggt ggcccttctt
    20701 tattaaaagc tgaccgttta ccgatggcaa cggcacttat tattggcaat aaaacctggt
    20761 taattgatgc tggttacggg gccagcttac aactggtaaa aaataatatt ccactgcgcc
    20821 agattgataa tattttactc acccatttac acagtgatca tattctggac tatccatcag
    20881 tcttaatgaa tgcatgggct agcggtctgc aggacaaaac tattcatgta ttcggtccgc
    20941 caggcacccg agccatgacc gctgccagtt ggcaggtctt cgatcgggac atcacactgc
    21001 gtatggaaga agaaggaaaa cccgatcctc gcaaactcgt tacagctcaa gatgttggtg
    21061 aaggcgtagt ttataaagat gataccgtga ccgtgactgc attaaaggtc cctcattctc
    21121 ctttccctga cggtgaggct tttgcttatc gcttcgacac acaaggcaag cgaattgtct
    21181 tctcaggaga tacctcgtac tttcccccac tggcaacttt ctcagaaggc gctgatatac
    21241 ttgtgcatga ggccgtacat gagccctccg tcgctaagct ggcagacagt attggcaatg
    21301 gtaaaacact ggcaaaagcc attgcttctc accatacgac tattgaggat gtaggcaaaa
    21361 ttgccaatga agcaaaagtg aaaaaactca tccttagcca cctggtacca atcaccgttc
    21421 cggatgaaac atggaagcaa gaagcacaga aaactattca ggtcctatcg tcgttggtca
    21481 tgaaaatatg acgattacca ttccattcgc cccataaaaa attccgtccc cgttagccct
    21541 tgttagcggg gaatattatg atttgcctta ctgtaagttg atcacgatcg cagtttacta
    21601 tttaactcca ccttgctgtc gaaaagtacc aacgttcgcc tgaaacgtct cttcccgaca
    21661 accgcgttca acgcttcaat taaataacaa aaacaacaca caaaattaac aatcgtattc
    21721 cgattaatac tgtagaggtc gacatgaaac cagaagattt ccgcgccagt acccaacgtc
    21781 ctttcaccgg ggaagagtat ctgaaaagcc tgcaggatgg tcgcgagatc tatatctatg
    21841 gcgagcgagt gaaagacgtc accactcatc cggcatttcg taatgcggca gcgtctgttg
    21901 cccaactgta cgacgcgctg cacaaaccgg agatgcagga ctctctgtgt tggaacaccg
    21961 acaccggcag cggcggctat acccataaat tcttccgcgt ggcgaaaagt gccgacgacc
    22021 tgcgccagca acgcgacgcc atcgctgaat ggtcacgcct gagctatggc tggatgggcc
    22081 gtaccccaga ctacaaagcc gctttcggtt gcgcactggg cgcgaatccg ggcttttacg
    22141 gtcagttcga gcagaacgcc cgtaactggt atacccgtat tcaggaaact ggcctctact
    22201 ttaaccacgc gattgttaac ccaccgatcg tcatttgccg accgataaag tgaaagacgt
    22261 ttacatcaag ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt
    22321 tgccaccaac tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat
    22381 gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtgaa
    22441 attaatctcc cgcgcctctt atgagatggt cgcgggtgct accggctcgc catacgacta
    22501 cccgctctcc agccgcttcg atgagaacga tgcgattctg gtgatggata acgtactgat
    22561 cccatgggaa aacgtgctga tctaccgcga ttttgatcgc tgccgtcgct ggacgatgga
    22621 aggcggtttc gcccgtatgt atccgctgca agcctgtgtg cgcctggcag tgaaactcga
    22681 cttcattacg gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg
    22741 tgtgcaggcc gatctcggtg aagtggtggc gtggcgcaac accttctggg cattgagtga
    22801 ctcgatgtgt tcagaagcga cgccgtgggt caacggggct tatttaccgg atcatgccgc
    22861 actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattataga
    22921 acgcaacgtt accagtggcc tgatctacct cccttccagt gcccgtgacc tgaacaatcc
    22981 gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg aacggtatgg atcacgtcca
    23041 gcgcatcaag atcctcaaac tgatgtggga cgccattggc agcgaatttg gtggtcgtca
    23101 cgagctgtat gaaatcaact actccggtag ccaggatgag attcgcctgc aatgtctgcg
    23161 ccaggcacaa agctccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc
    23221 ggaatacgac cagaacggct ggactgtgcc gcacctgcac aacaacgacg atatcaacat
    23281 gctggataag ctgctgaaat aacgcagcag gaggttaaga tgcaattaga tgaacaacgc
    23341 ctgcgctttc atgacgcgat ggccagcctg tcggcagcgg taaatattat caccaccgag
    23401 ggcgacgccg gacaatgcgg gattacggca acggctgtct gctcggtcac ggatacacca
    23461 ccgtcgctga tggtgtgcat taacgccaac agtgcgatga acccggtttt tcagggcaac
    23521 ggcaagttgt gcgtcaacgt cctcaaccat gagcaggaac tgatggcgcg ccacttcgcg
    23581 ggcatgacag gcatggcgat ggaagagcgt tttagcctct catgctggca aaaaggtccg
    23641 ctggcgcagc cggtgctaaa aggttcgctg gccagtcttg aaggagagat ccgcgatgtg
    23701 caggcgattg gcacacatct ggtgtatctg gtggagatta aaaacatcat cctcagtgca
    23761 gaaggtcacg gacttattta ctttaaacgc cgtttccatc cggtgatgct ggaaatggaa
    23821 gctgcgattt aagctccagc cccttcacgc attcgtcgaa ggggcatttt tattgtgatc
    23881 tcaatcacat atctacccga tgaataattc ttgcccaacc gctcaccgcc attcctgacc
    23941 gcttacatcc ctaaaataac cactcagtta tttaccttac tttacgcgca cgtatctctg
    24001 gcaacatcac tgcaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac
    24061 ggcttcggcc tatgattaac aaattactct atggattttg gtttgcagga aggcagcaag
    24121 tgagtgaatc cccgggagct tacaaaagta agtgtcagag gtgaacgaac gcagctgccg
    24181 cacctgtaag ccaaaagacg acgagtaaaa tgccaggttt tactatggat acgaaaaaac
    24241 tattcaagca cataccctgg gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag
    24301 ttgcattacg tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc tctgtgtcgg
    24361 tgtatctggt ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg atgaaactcg
    24421 accccacgcg cgcgacgcct gcggttatta acaacgacgg tctgaactac gttccgacca
    24481 accgttacgt gttgtttggt caccacttcg ccgctatcgc cggtgctggt ccgctggtgg
    24541 gaccggttct cgccgcgcag atgggctacc tgcctggcac gctgtggctg ctggcggggg
    24601 tagtgctggc cggtgcggta caggacttta tggtgctgtt tatctcctct cgccgtaacg
    24661 gcgcatctct cggtgagatg atcaaagaag agatggggcc agtaccgggg actatcgcgc
    24721 tgtttggctg tttcttaatc atgatcatca tcctcgccgt tctggcgctg attgtggtta
    24781 aagccctggc cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc
    24841 tgtttatggg tatctacatg cgctttatcc gtccggggcg tgtgggtgaa gtctctgtta
    24901 ttggtatcgt gctgctggtt gcctctatct acttcggtgg cgtgattgct cacgatccgt
    24961 actggggtcc ggcactgacc tttaaagaca ccaccattac cttcgcgctg attggctatg
    25021 cgtttgtttc cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa
    25081 ccttcctgaa aatcggcgtt atcgtcggcc tggcgctggg tatcgtggtg ctgaacccgg
    25141 aactgaaaat gcctgcgatg acccagtaca ttgacggtac tggcccgctg tggaaaggcg
    25201 ctctgttccc gttcctgttc atcaccatcg cctgtggtgc ggtatctggc ttccacgcgc
    25261 tgatctcttc cggtacgacg ccgaaactgc tggctaacga aaccgacgcg cgtttcattg
    25321 gctacggcgc aatgctgatg gagtccttcg tggcgattat ggcgctggtt gcagcgtcca
    25381 tcatcgaacc gggtctttac ttcgcgatga acaccccgcc tgctggcctt ggcatcacca
    25441 tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat catcatggcg cagctgaaag
    25501 acgttaccgc acacgcggca gcgaccgtca gctcctgggg cttcgtgatt tcgccagagc
    25561 agatcctgca aaccgcgaaa gacattggtg agccttctgt cctgaaccgt gcaggtggtg
    25621 cgccgacgct ggcggtaggt atcgctcacg tgttccacaa agtgctgccg atggctgaca
    25681 tgggcttctg gtatcacttc ggtattctgt tcgaagccct gttcatcctg accgcgctgg
    25741 atgcgggtac ccgttctggc cgctttatgc tgcaagacct gctgggtaac ttcatcccgt
    25801 tcctgaaaaa aaccgactct ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc
    25861 tgtggggcta cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt
    25921 ggccgctgtt cggtatctcc aaccagatgc tggcagccgt agcgctggta ctgggcaccg
    25981 ttgtgctgat taagatgaag cgcacccaat acatctgggt aactgttgtt ccggctgtat
    26041 ggctgcttat ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc aacccgcaga
    26101 tggaaggctt cttctacatg gcaagccagt acaaagagaa gattgctaac ggtactgacc
    26161 tgacggcgca gcagattgcc aacatgaacc acatcgttgt gaacaactac accaacgcag
    26221 gcctgagtat tctgttcctg attgtggtgt acagcatcat cttctacggt ttcaaaacct
    26281 ggcttgcggt gcgtaacagc gacaaacgta ctgacaaaga aacaccgtac gttccaatcc
    26341 cggaaggcgg cgtgaagatc tcttcgcacc actaaccgtg tttagccccg cttcggcggg
    26401 gctttgttct tatcagagtg aactatgttt ggtaacttag gacaggcaaa aaaatatctc
    26461 ggccaggcgg cgaagatgtt gattggtatt ccagactacg acaactacgt cgagcatatg
    26521 aagaccaacc atcccgacaa gccgtacatg agctatgaag aattcttccg cgaacgccag
    26581 aacgcgcgct acggcggcga tggtaaaggc ggtatgcgct gttgttaatg gagagagcat
    26641 gaacccgatt gcagttaccc tactcaccgg ttttttaggc gcgggcaaaa ctaccctgct
    26701 gcgccatatt cttaacgaac aacacggcta caagattgcc gtgattgaaa acgaattcgg
    26761 cgaagtctcc gttgatgacc aactgattgg cgaccgcgcc acgcagatca aaacgctgac
    26821 caacggctgc atctgctgtt cgcgctccaa cgagctggag gacgcactac tcgacctgct
    26881 ggataatctc gacaagggca atattcagtt cgatcgtctg gtgattgaat gcaccggcat
    26941 ggccgatccc ggcccgatta ttcagacctt tttctcccat gagattttat gccagctcta
    27001 cctgctggac ggcgtgattg cgctggtgga tgcggtacat gccgatgagc agatgaacca
    27061 gttcaccatc gcccagtccc aggtgggcta cgccgaccgt attctgctga ctaaaaccga
    27121 cgtcgcaggc gaagcagaga aactgcgtga acgcctggcg cgcatcaacg cccgcgcacc
    27181 ggtctacacc gtcacccacg gcgacatcga cctgggcctg ctgttcaaca ccaacggttt
    27241 tatgctggaa gagaacgtcg tcagcaccaa accgcgtttc cactttatcg cggataaaca
    27301 aaacgatatt tcgtcaattg tggtggaact ggattacccg gtagatatca gcgaagtttc
    27361 ccgcgtgatg gaaaacctgc tgctggaatc ggcggataaa ctgctgcgtt acaaagggat
    27421 gctgtggatt gacggcgaac ctaaccgcct gctgttccag ggcgtccagc gcctctacag
    27481 cgccgactgg gacaggccgt ggggcgatga aaaaccgcat agcacaatgg tgtttatcgg
    27541 gattcagttg ccggaggaag agattcgggc ggcgtttgcg ggattgagga agtaagttcg
    27601 ggaaagatca actttttgag agttccaccg caacattatt acgagtggaa ctctttagat
    27661 atgtcagacc aaccaaagct tcttcaattg cgttttcatt ttttcatgtc tcattttcag
    27721 ttcagccaat ccccattctg aagaattaca aacatctttt gttaaataaa aagagctttt
    27781 actattaagt ttctcataaa tagatttctt tttgataaaa tcatagtgtt gtgtttcaga
    27841 atttttggaa ccacttatga gtgttaaatt acctatttta tgcagccagt agacatgctc
    27901 ttgaggttgg aatctattaa tccagtattc attaactaat gcttgaggaa gaatatgctc
    27961 aattgtgatt ctccccgtat aggttttaat cacgctctca tcttgttgct ccatatctaa
    28021 gcgaagcaaa attgctttta ttaagttcac ttgattcgga cgaggctcat ataaatcttc
    28081 atccagagca gctataaaac ctgaattatc tgcatgttgg tttatctgat ttatcacaga
    28141 atcaaaaggc atatcattat ttattgctac cagagcagaa taacaaacca tttctctttg
    28201 gcttttaatt tgttttttta accacccatg catatatacc ttctcaaagg cggtaataaa
    28261 ttgagaaaaa tcatctaaat taaaatcttc tgtacgagcc attcgattca tgaatgccat
    28321 aaccggagga atccactcat ctacccctaa attacttagc gatgctattt ttcgtcgtat
    28381 tgaagggtgt tcaaaatcat tctctaaaat cttagtataa tt
//
LOCUS       DAETQL010000052        28407 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.052, whole genome shotgun sequence.
ACCESSION   DAETQL010000052 DAETQL010000000
VERSION     DAETQL010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.052"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="K0W65_002430"
     CDS             complement(<1..142)
                     /gene="yjdI"
                     /locus_tag="K0W65_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HBI3095660.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            323..1954
                     /gene="dcuS"
                     /locus_tag="K0W65_002431"
     CDS             323..1954
                     /gene="dcuS"
                     /locus_tag="K0W65_002431"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBI3095661.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1951..2670
                     /gene="dcuR"
                     /locus_tag="K0W65_002432"
     CDS             1951..2670
                     /gene="dcuR"
                     /locus_tag="K0W65_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBI3095662.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3241..4581
                     /gene="dcuB"
                     /locus_tag="K0W65_002433"
     CDS             3241..4581
                     /gene="dcuB"
                     /locus_tag="K0W65_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HBI3095663.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4659..6305
                     /gene="fumB"
                     /locus_tag="K0W65_002434"
     CDS             4659..6305
                     /gene="fumB"
                     /locus_tag="K0W65_002434"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBI3095664.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6428..7057
                     /locus_tag="K0W65_002435"
     CDS             6428..7057
                     /locus_tag="K0W65_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBI3095665.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7196..8617)
                     /gene="melB"
                     /locus_tag="K0W65_002436"
     CDS             complement(7196..8617)
                     /gene="melB"
                     /locus_tag="K0W65_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HBI3095666.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8720..10075)
                     /gene="melA"
                     /locus_tag="K0W65_002437"
     CDS             complement(8720..10075)
                     /gene="melA"
                     /locus_tag="K0W65_002437"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBI3095667.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10358..11266
                     /gene="melR"
                     /locus_tag="K0W65_002438"
     CDS             10358..11266
                     /gene="melR"
                     /locus_tag="K0W65_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBI3095668.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11465..13732
                     /gene="adiA"
                     /locus_tag="K0W65_002439"
     CDS             11465..13732
                     /gene="adiA"
                     /locus_tag="K0W65_002439"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBI3095669.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14057..14818
                     /gene="adiY"
                     /locus_tag="K0W65_002440"
     CDS             14057..14818
                     /gene="adiY"
                     /locus_tag="K0W65_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HBI3095670.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14955..16292
                     /gene="adiC"
                     /locus_tag="K0W65_002441"
     CDS             14955..16292
                     /gene="adiC"
                     /locus_tag="K0W65_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBI3095671.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16396..18039
                     /gene="eptA"
                     /locus_tag="K0W65_002442"
     CDS             16396..18039
                     /gene="eptA"
                     /locus_tag="K0W65_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBI3095672.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18036..18704
                     /gene="basR"
                     /locus_tag="K0W65_002443"
     CDS             18036..18704
                     /gene="basR"
                     /locus_tag="K0W65_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBI3095673.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18705..19805
                     /gene="pmrB"
                     /locus_tag="K0W65_002444"
     CDS             18705..19805
                     /gene="pmrB"
                     /locus_tag="K0W65_002444"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBI3095674.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19781..19870)
                     /gene="pmrR"
                     /locus_tag="K0W65_002445"
     CDS             complement(19781..19870)
                     /gene="pmrR"
                     /locus_tag="K0W65_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBI3095675.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19982..21484)
                     /gene="proP"
                     /locus_tag="K0W65_002446"
     CDS             complement(19982..21484)
                     /gene="proP"
                     /locus_tag="K0W65_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBI3095676.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21748..22626)
                     /locus_tag="K0W65_002447"
     CDS             complement(21748..22626)
                     /locus_tag="K0W65_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HBI3095677.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEGLRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22623..24851)
                     /gene="crfC"
                     /locus_tag="K0W65_002448"
     CDS             complement(22623..24851)
                     /gene="crfC"
                     /locus_tag="K0W65_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBI3095678.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25254..25589
                     /locus_tag="K0W65_002449"
     CDS             25254..25589
                     /locus_tag="K0W65_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBI3095679.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25747..26190
                     /gene="yjdN"
                     /locus_tag="K0W65_002450"
     CDS             25747..26190
                     /gene="yjdN"
                     /locus_tag="K0W65_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HBI3095680.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26323..27111
                     /gene="phnC"
                     /locus_tag="K0W65_002451"
     CDS             26323..27111
                     /gene="phnC"
                     /locus_tag="K0W65_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBI3095681.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27136..28152
                     /gene="phnD"
                     /locus_tag="K0W65_002452"
     CDS             27136..28152
                     /gene="phnD"
                     /locus_tag="K0W65_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3095682.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28258..>28407
                     /locus_tag="K0W65_002453"
     CDS             28258..>28407
                     /locus_tag="K0W65_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HBI3095683.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMATF"
BASE COUNT         7297 a         7421 c         6935 g         6754 t
ORIGIN      
        1 acgactttcg tttgagatta aataacttgc cgttgccacg tacgcaattg ccagaatgct
       61 gacatatcgc agtgttgaaa tagacatcga tcttttcgcc ggtataacag cgataacccc
      121 cgtccagtag cgcctgatcc atgaccttcc ctctctcatc agtatgatta tcgctataag
      181 catagccccg gaatcttcat acagcactgg cagtttccgt tgccagagtc tcttccccta
      241 tatagccaat catttttact ccctttgaat tacccgcctc atcagagata atgcttaaga
      301 aatttgtcac acaaggaagc tgatgagaca ttcattgccc taccacatat tacgcaaacg
      361 tccgatgaaa ttgagtacca ctgtgatctt aatggtcagc gcggtactgt tctcggtgct
      421 attggtggtg catctgattt acttctcgca aatcagtgat atgacgcgag acgggctggc
      481 caacaaggca ctggcagtgg cgcgtaccct cgccgactcg ccggaaatcc gtcagggctt
      541 gcagaaaaaa ccgcaggaga gtggcatcca ggccatcgcg gaagccgtgc gcaaacgcaa
      601 cgatctgctg tttattgtcg ttaccgatat gcaaagtctt cgctactcac atcctgaagc
      661 ccagcgtatt ggtcagccat ttaaaggtga tgacatcctt aatgcgctga atggcgaaga
      721 aaatgtcgct atcaatcgcg gttttctggc gcaggcttta cgcgtattta cccccatcta
      781 cgatgaaaat cataaacaaa ttggcgtggt ggcgatcggc cttgagttaa gccgcgtgac
      841 ccaacagatc aatgacagtc gctggagcat tatctggtcg gtattatttg gcatgctggt
      901 cggactgatt ggcacctgca ttctggttaa ggtactgaaa aaaatccttt tcggcctgga
      961 accctacgaa atctccacgc tgtttgagca acgccaggcc atgttgcagt ctatcaaaga
     1021 aggcgtcgtt gccgtggacg atcgcggcga ggtcacgctg atcaacgatg ccgcacaaga
     1081 attgctgaat taccgtaagt cgcaggacga tgagaaactg tcgacgctca gccactcatg
     1141 gtcacaggtg gtagatgtct cggaagtgtt acgcgacggt acgccgcgcc gcgacgaaga
     1201 gattacgatt aaagaccggc tattactgat caacaccgtt ccggtgcgca gtaatggcgt
     1261 tatcatcggt gccatttcaa ccttcaggga caaaactgaa gttcgtaaac tgatgcagcg
     1321 actcgacggt ctggtcaact atgctgacgc acttcgtgaa cgatcccacg aatttatgaa
     1381 taaattgcat gtgattctcg gattattgca tctgaagagt tataagcagt tggaagatta
     1441 cattctcaaa acagccaata actatcagga agagattggc tctctgctgg gtaagatcaa
     1501 atctccggtt atcgctggtt ttttaatcag caagattaac cgcgcgaccg atttaggcca
     1561 tacgctgatt ttaaacagtg aaagccagct gccagacagc ggcagtgagg accaggtcgc
     1621 gacgctgatt accacattgg gaaatctgat agaaaacgcg ctggaagcat tagggccgga
     1681 acccggtggc gaaattagcg taacattgca ctaccgtcac ggctggctgc actgcgaagt
     1741 taacgatgat ggaccgggga tcgcacccga caaaatcgat cacatttttg acaaaggtgt
     1801 ctcgacaaaa ggaagcgagc gaggcgtcgg tttagcactt gtcaaacaac aggtagaaaa
     1861 tctcggcggc agcatcgccg tggaatcgga accggggatt ttcacacaat tttttgtcca
     1921 gataccctgg gacggggaga ggtcgaacag atgatcaatg tattaattat cgatgacgac
     1981 gcaatggtcg cggagctgaa tcgccgatac gtagcacaaa tcccaggctt tcaatgctgt
     2041 ggaacagcct cgacgctgga gaaagccaaa gagattatct tcaatagcga tgcgcctatc
     2101 gacctgatat tgctcgatat ctatatgcaa aaagagaacg ggctcgattt actgcctgtc
     2161 ctgcataacg cgcgttgcaa aagtgatgtg attgtcatct cctccgcagc cgatgcggca
     2221 accattaaag attcgctgca ttacggtgtc gtggattacc tgatcaaacc cttccaggct
     2281 tcccgctttg aagaggcgct caccggctgg cggcaaaaga aaatggcgct ggaaaaacat
     2341 cagtattatg accaggccga gcttgatcag ctaatccacg gcagcagctc caacgaacag
     2401 gatcctcgtc gcttgccaaa aggcttaacg ccgcagacgt tacgcacgct gtgccagtgg
     2461 attgacgcgc atcaggacta tgagttttca accgacgagc tggctaacga ggttaacatc
     2521 tcgcgtgttt cctgccgaaa atacctcatc tggctggtca actgccacat cttgttcacc
     2581 agtatccatt atggcgtcac ggggcgtccg gtttaccgtt accgtattca ggcagaacac
     2641 tactcactgc tgaaacaata ttgccaataa cggatcgctg gttatctgta agtaataatt
     2701 attaccgtaa tgcaaaatac ccctggcaca agttacatcg ttaatcagta aaacttgtgc
     2761 cagatcaaat ataattatcc ctccatcatc gttaaaaatt aatatctctt cagatgaacg
     2821 gtgtttttaa tttcaaaacg ataacaaaag ttaattaact attatgtcac ccgcattatg
     2881 tgtattttta cccacaaatg ggtagatcag attaatctat aaacctaatg atatctgccc
     2941 tgagaacaaa aaatagaccg ataaatatca ataagataac agcaaacaaa acattaacat
     3001 ctgcgcagta caaactataa acccatcgcc agagagtttt tctctctgaa aaagccgctt
     3061 atcacagtgc ataaatttgc cgctgcttta atcagccaat attcactgtg aggtatttgc
     3121 taaagccggt aacgaccaaa cggatattta gtcaggctct gaaaacagtt catacaaaac
     3181 agaacgtgac tgtgatctat tcagcaaaaa tttaaatagg attatcgcga gggttcacac
     3241 atgttattta ctatccaact tatcataata ctaatatgtc tgttttatgg tgccagaaag
     3301 ggtggtatcg cgctgggttt attaggcggt atcggtctgg tcattctggt cttcgtcttc
     3361 caccttcagc caggtaaacc accagttgat gtcatgctgg ttatcattgc ggtggtggcg
     3421 gcatcggcga ccttgcaagc ttcgggcggt cttgatgtca tgctgcaaat tgccgagaag
     3481 ctgctgcgcc gcaacccgaa atatgtctca attgtcgcgc cgtttgtgac ctgtacactg
     3541 accattcttt gcggtacggg tcatgtggtt tacaccattc tgccgatcat ctacgacgtc
     3601 gccattaaga acaacatccg tccggaacgt ccgatggcgg caagttctat cggtgcacag
     3661 atggggatta tcgccagtcc ggtgtcggtt gcggtcgtgt ctctggttgc gatgctgggt
     3721 aatgtcacct ttgatggtcg ccatcttgag ttcctcgatc tgctggcaat caccattcca
     3781 tcgacgttaa tcggtatcct ggcgatcggt atcttcagct ggttccgcgg taaagatctg
     3841 gataaagacg aagagttcca gaaattcatc tccgtaccgg aaaaccgtga gtatgtttac
     3901 ggtgataccg cgacgctgct ggataaaaaa ctgccgaaaa gcaactggct ggcaatgtgg
     3961 attttcctcg gggcaatcgc tgtagtcgcc cttcttggtg ctgattcgga cctgcgtcca
     4021 tccttcggcg gcaaaccgct gtcgatggta ctggttattc agatgtttat gctgctgacc
     4081 ggggcgctga ttattatcct gaccaaaacc aatcccgcgt ctatctcaaa aaacgaagtc
     4141 ttccgttccg gtatgatcgc catcgtggcg gtgtacggta tcgcatggat ggcagaaacc
     4201 atgttcggtg cgcatatgtc tgaaattcag ggcgtactgg gtgaaatggt gaaagagtat
     4261 ccgtgggcgt atgccattgt tctgctgctg gtttccaagt ttgtaaactc tcaggctgcg
     4321 gcgctggcgg cgattgttcc ggtcgcgctg gcgatcggcg ttgatccggc atacatcgtg
     4381 gcttcagcac cggcttgcta cggttattac atcctgccga cttatccgag cgatctggca
     4441 gcgattcagt ttgaccgttc cggcaccacc cacatcggtc gcttcgtcat caaccacagt
     4501 tttattctgc cggggttgat tggtgtgagc gtatcgtgcg tcttcggctg gatcttcgcc
     4561 gcgatgtacg ggttcttata aatgcacttt gcgtgccgcc cgtgactacg cggcacgcca
     4621 ttttcgaata acaaatacag agttacaggc tggaagctat gtcaaacaaa ccctttatct
     4681 accaggcacc tttcccgatg gggaaagaca ataccgaata ctatctactc acttccgatt
     4741 acgttagcgt tgccgacttc gacggcgaaa ccatcctgaa agtggaacca gaagccctga
     4801 ccctgctggc gcagcaagcc tttcacgacg cttctttcat gctacgtccg gcacaccaga
     4861 aacaggttgc ggctattctt cacgatccag aagccagcga aaacgacaag tacgtggcac
     4921 tgcaattctt aagaaactcc gaaatcgccg ccaaaggcgt gctgccgacc tgccaggata
     4981 ccggcaccgc gatcatcgtc ggtaaaaaag gccagcgcgt gtggaccggt ggcggtgatg
     5041 aagaagcgct gtcgaaaggc gtctataaca cctatatcga agataacctg cgctactcgc
     5101 aaaacgcggc gctggacatg tacaaagagg tcaacaccgg cactaacctg cctgcgcaaa
     5161 tcgacctgta cgcggtagat ggcgatgagt acaaattcct ttgcgttgcg aaaggcggcg
     5221 gctctgccaa caaaacgtat ctctaccagg aaaccaaagc cctgctgact cccggcaaac
     5281 tgaaaaactt cctcgtcgag aaaatgcgta ccctcggtac tgcagcctgc ccgccgtacc
     5341 atatcgcgtt tgtgattggc ggtacgtctg cggaaaacaa cctgaaaacc gtcaaattag
     5401 caagcgctca ctattacgat gaactgccga cggaagggaa cgaacatggt caggcgttcc
     5461 gcgatgtcca gctggaacag gaactgctgg aagaggccca gaaactcggt cttggcgcgc
     5521 agtttggcgg taaatacttc gcgcacgaca ttcgcgttat ccgtctgcca cgtcacggcg
     5581 catcctgccc ggtaggcatg ggcgtctcct gctccgctga ccgtaacatt aaagcgaaaa
     5641 tcaaccgcga aggtatctgg atcgaaaaac tggaacacaa tctaggtcag tacattccac
     5701 aagaactgcg tcaggccggt gaaggcgaag cggtgaaggt cgaccttaac cgcccgatga
     5761 aagagatcct cgcccagctt tcgcaatatc cggtttccac tcgtttgtcg ctcactggca
     5821 ccattattgt gggtcgagat attgcacacg ccaagctgaa agagctgatt gacgccggta
     5881 aagaacttcc gcagtacatc aaagatcacc cgatctacta cgcgggtccg gcgaaaaccc
     5941 ctgccggtta tccatcaggt tcacttggcc caaccaccgc aggtcgtatg gactcctatg
     6001 tggatctgct gcaatcccac ggcggcagca tgatcatgct ggcgaaaggc aaccgcagcc
     6061 agcaggttac cgacgcgtgt cataaacacg gcggcttcta cctcggtagc atcggcggtc
     6121 cggcggcggt actggcgcag cagagcatca agcatctgga atgcgtcgct tatccggagc
     6181 tgggtatgga agctatctgg aaaatcgaag tagaagattt cccggcgttt atcctggtcg
     6241 atgacaaagg taacgacttc ttccagcaaa tcgtcaacaa acagtgcgcg aactgcacta
     6301 agtaacctct tcggcccagc gcctggcagc atgctgccag gtgatccccc tgggccacct
     6361 cttttgcgat tgtaatttca tgcttgctgg tgaatagtca gtattttccc tgacttgcga
     6421 actcaccatg acccgcacac tcaagccgtt aattcttaac actggcgcac tggcgctaac
     6481 gttaatcctg atttataccg gcatttcggc ccatgacaaa ctcacctggc tgctggaagt
     6541 gacaccggtg attatcgtcg tgccgctact acttgccacc gccagacgtt acccgttaac
     6601 gctgctgctc tatacgctca tcttctttca cgccatcatc ctgatggtag gcgggcagta
     6661 cacctacgcg aaagtccccg ttggttttga ggtgcaggaa tggctggggt tgagtcgtaa
     6721 tccgtatgac aagctggggc actttttcca ggggctggtg cctgcactgg tggcacgaga
     6781 aattctcgtt cgcgggatgt acgtgcgcgg acgtaaaatg gtggcgtttc tggtctgttg
     6841 cgtggcgctg gcgataagcg cgatgtatga attaatcgag tggtgggcgg cactggcgat
     6901 gggccaggga gcggatgatt ttctcggtac ccagggcgac cagtgggaca cgcaatccga
     6961 tatgttctgc gcgctgcttg gcgcattaac gacagtgata ttgctcgctc gctttcattg
     7021 ccgccagcta cggcgctatg gcttgataac tggataacaa cgtgcacttt gttgatgccg
     7081 gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc attacattaa
     7141 gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt ccacgtcagg
     7201 ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg ggcggtactt
     7261 tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca ttgaggcgat
     7321 agaaacggaa gtagagaatc agcgttacca tgaaaaacag agtcggtaga gcaatcataa
     7381 taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca tagccaatca
     7441 tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc accaccatag
     7501 tctgcacgga gtaagcgatg ctttcacagc gtacgtgcag tttatattca ccgtaatcaa
     7561 cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc acattcagca
     7621 aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc attaacagga
     7681 gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg cgtcgggata
     7741 atgatttaac taagcgtggg aagaatacta acgtcaccag gttagcagct cccgcatacg
     7801 acagataata ggggaacaaa tccgcatcac cgataacata tgagaaataa tagatagcaa
     7861 atccggtaat aatgttgctg gctacattat aagcaagagc catacccaag aggcatgaaa
     7921 gctgatcgtt tttataaata agcgcaacga tggctttaag tgtcagatgg cttccttcag
     7981 cagacgattg attgtctgac gaaaagactt catgcacatt gcgcagagtg atgatggttg
     8041 aaacaataaa aaaggcgatc aataccagag tgaacatctg aaagccaaat ccccgatcgc
     8101 caccgccgac ataattaaca aatggtagcg tcacacctgc cgtaacaaag cctgccagac
     8161 tggcaaaaaa acgcggataa ggaaccagtt gttcgcgttc acgtttatcg agcgtgatgg
     8221 ttggaaccag cgaccagaag ggaatatcca taatggtgta agtcatgccc cagaggatgt
     8281 aggtcacgca aacaaagaca atctgagtag taccttcaaa cagatgcgca ctaaagagga
     8341 gaaataagat tacagagttt gccaacgtac cgatcaggat ccagggtttg aacttacccc
     8401 atcgcgatcg cgtagcattt acaatccatc ccataatcgg atcgttaata gcatcccaga
     8461 tcctcgccac cagaaacaaa gtaccgacca aacccacaga cagcccgacg acatcggtgt
     8521 agtaatacat gaggtacata tacacaatgc cgatcgcaaa atccttcccg aacgctccaa
     8581 atccataact gagttttgta gtcattgaaa tgctcatagg gtatcgggtc gctgttacca
     8641 acgcccgcct tctgttattg gtatcgtcag aggtgtgcgc cggaataaat cccccggcgc
     8701 agtagcgttt agtcgcgttt taacggtgca accagcttgg cagccagtcg ccgtgggcgg
     8761 caatcaggtc gtcaacaaga gcatatattt cgtcaatgcc cagcacggcg gcagtatgcg
     8821 ggtccatcat cgcggcgtgg taaacacggt cgcgattttc cgtaagaata gcttcggtca
     8881 gcagcgtctg tacgttgatg ttggtttgca tcagggcggc cagatgcgaa ggtagcgtac
     8941 cgactttggt cggctgaatg ccattagcat caaccagaca ggctacttcc acgcaacatc
     9001 cttgtggcag gttatcaatc aaaccatcgt tacggacgtt gccgtaaatc acactcggct
     9061 cgccagtcca gatagcgttc atgattgtgc tggcatattc ccgtgacggt ttaatatcaa
     9121 tccgggaggc gtttttatac tcctccagct ctttatgcca gttcgccagc tgctcgacgc
     9181 agcgtttcgg gtactcatcc agcggtactt tataacgctc aatcaaatcc tcacgaccta
     9241 gcttaataaa ccacggcgta tattcggcaa aatgttctga tgattccgtg acgaagtagc
     9301 ccagcttttt gaacatttcg tagcgcacaa tattctggca gcgagtatta ccatgaatat
     9361 ttggcttcgg tgcctgccct gcttcataag ccgccagcag ttccgggtag agattcacgt
     9421 aactgccgtc ggcggttttg cgctccagct ccaggtaaaa cgccatatgg ttgatacctg
     9481 cgcaacggta acgcagcgta gccgggtcga tattgaggtc acgcgccagc tcttccgccg
     9541 ttccctgcac cgaatggcac agcccgacct gtttgatata cggatagcgg gcatacatcg
     9601 cccaggtatt catcgccatt gggttaacat agttgagcat ggtggcatcg gggcagactt
     9661 ccgtcatgtt ctcgcaaatt tgccacagat gcggaatggt acgtagcgcg cgcataatac
     9721 cgcccggccc caacgtatcg gcaatggttt gttccagacc atgccgctta cagacctcga
     9781 aatcagtcac cgtgcaaggt tcataaccgc caatctgaaa tgccaccacg acaaaatcgg
     9841 catcctctaa ggcttctttc tgttgggtgt ggcaggtgat tttgccgctg gcccctgctg
     9901 aatccatcag cttacgcacc acaatatgcg actcttccag gcgggtgggg tcaatgtcca
     9961 tcagggcaat atgcgccgtt ttcagcgcct cgcgatggaa cacatcacca agaatatttt
    10021 taacgaaaat cgtcgaacca gcgccgataa atgtaatttt gggtgcagac atcatgcaga
    10081 tctcctggct tgcttgaata acttcatcat ggcaggcgaa tctgcagaaa acctccggct
    10141 tccaggttga gcattcccat aaactcagat cctgcgtgaa gcagcagtaa atctgagttt
    10201 atgggaataa attacctgaa aagtagagtt ttcggcctca tgatgctcgg acagtcccct
    10261 cgcccctctg ggaagagggt gaaaaccgtg ctcccactcg cagtcatcct ccctcactcc
    10321 tgccataatt ctgatattct aggaaagaga gccatccatg aatacagata cgtttatgtg
    10381 cagcagcgac gaaaaacaga cccgcagccc gctgtcgctg tattcggaat accagcggat
    10441 ggagattgag tttcgcgcac cgcatatcat gcccaccagc cactggcatg gtcaggtcga
    10501 agtgaatgtg cctttcgatg gcgatgtgga atacctgatc aacaatgaaa aagtgaatat
    10561 caatcagggt catatcacgc tgttctgggc ctgtacaccg caccaactaa cagataccgg
    10621 aacctgtcag agcatggcga tttttaatct gccgatgcat ctgtttctct cctggccgct
    10681 ggataaagac ctgattaacc acgtcactca cggcatggtg atcaaatcac ttgccacaca
    10741 gcaacttagc ccgtttgaag tacgccgctg gcagcaggaa ttaaacagtc cgaatgagca
    10801 aattcgccag ctcgccattg atgaaattgg cctgatgctc aagcgattta gcctctctag
    10861 ctgggaaccg attctggtca ataaaacctc gcgcacacac aaaaacagcg tctcgcgcca
    10921 tgcgcaattt tatgttagcc agatgctggg ctttattgcc gaaaactatg atcaggcgct
    10981 gaccatcaac gatgtggctg agcacgtcaa acttaacgcc aactatgcaa tggggatatt
    11041 ccagcgggtc atgcaattga cgatgaaaca gtacattacc gcgatgcgca tcaaccacgt
    11101 tcgcgcgtta ctgagcgata ccgataaaag tattctcgat attgccctga cggcaggctt
    11161 tcgttcaagt agtcgttttt acagcacgtt cggcaaatat gtcggcatgt cgccgcaaca
    11221 ataccgcaaa cttagccaac agcgccgcca gacgtttccc ggctaaagac gatatcagta
    11281 tcagccaaaa aaatagttta gccgtcgata ataagcagtg aaaataattt aaaataatca
    11341 cataacgtat tgtttataaa actaaatatt tcaaaaatgt ttgtttttca cgcgctttac
    11401 agcccgaaaa ggccggaaga tacttgcccg caacgaagat tccttcataa ccgggtaagc
    11461 aatgatgaaa gtattaattg ttgaaagcga gtttctccat caagacacct gggtcggtaa
    11521 cgccgttgag cgtctggcag atgctttaag ccagcaaaat gttaccgtga ttaaatccac
    11581 ctcctttgat gatggttttg ccattctctc ttcaaacgaa gccattgact gcctgatgtt
    11641 cagctatcaa atggagcatc cggacgaaca tcaaaacgtc agacaattga tcggtaagct
    11701 tcatgagcgc caacaaaacg tgccggtctt cctgttgggc gatcgggaaa aagccctcgc
    11761 cgcaatggac cgggatctgc tggagcttgt cgatgaattc gcctggattc tggaagatac
    11821 cgccgacttt atcgccggac gcgccgttgc cgcgatgacc cgctaccgcc agcagctgtt
    11881 gccgccactg ttcagcgcgc tgatgaaata tagtgacatc catgaatatt cctgggcagc
    11941 gccaggccac cagggcggcg ttggttttac caaaacaccc gccggacgtt tctaccatga
    12001 ctactatggt gaaaatctgt tccgtaccga catgggcatc gaacgaactt ccctcggttc
    12061 tttgcttgac catactggcg catttggcga aagcgaaaaa tatgccgcac gcgtatttgg
    12121 tgccgatcgc tcctggtcgg tagtcgtcgg tacttccggc tctaaccgca ccatcatgca
    12181 ggcttgcatg accgataacg atgtcgtggt cgttgaccgt aactgccata aatccatcga
    12241 acaaggtttg atgctgacag gcgcgaaacc ggtctatatg gtgccaagcc gcaaccgcta
    12301 cggcattatc gggccaatct atccgcagga aatgcaacct gaaaccttgc aaaagaaaat
    12361 cagtgaaagc ccgctgacca aagacaaagc cgggcaaaaa ccgtcttact gcgtggtaac
    12421 caactgcacc tatgacggcg tgtgttataa cgctaaagaa gcgcaggatc tgctggaaaa
    12481 aacctccgat cgtctgcact ttgacgaagc ctggtacggc tatgcacgtt tcaacccgat
    12541 ctatgccgat cactatgcca tgcgcggcga acctggcgat cacaacggtc ctaccgtttt
    12601 cgccacccac tccatccaca aactgctgaa tgcgctgtca caggcttctt atattcatgt
    12661 acgtgaaggt cgtggggcga ttaacttctc ccgcttcaac caggcctaca tgatgcatgc
    12721 caccacctcc ccgctgtatg ccatctgcgc atccaacgac gtggcggtgt cgatgatgga
    12781 cggcaacagc ggcctgtcac tgacacagga agtgattgac gaagcggttg atttccgtca
    12841 ggcgatggcg cggctatata aagagttcac cgctgacggt agctggttct tcaaaccgtg
    12901 gaacaaagaa gtcgtcaccg acccacaaac cggcaaaacc tatgactttg ctgacgcacc
    12961 aaccaaactg ctgaccaccg ttcaggactg ctgggtaatg catccgggcg aaagctggca
    13021 cggcttcaaa gatattccgg ataactggag tatgctcgac ccaattaaag tcagcatcct
    13081 tgctccggga atgggtgaag atggtgaact ggaagaaacc ggtgttccgg cggcgctggt
    13141 cactgcctgg cttggtcgcc acggcattgt gcctacccgc accactgact tccaaattat
    13201 gttcctgttc tctatgggcg taacccgtgg gaaatggggg actctggtta acaccctttg
    13261 ctccttcaaa caccactatg acgccaacac accgctggcg caggtgatgc cggaacttgt
    13321 tgaacaatat cctgacactt acgcgaacat ggggattcac gatctgggtg acaccatgtt
    13381 tgcctggctg aaagaaaaca accctggcgc acggttgaac gaagcctatt ccggcctgcc
    13441 ggtggcggaa atcaccccgc gtgaagcgta caacgcgatt gtcgacaaca atgtcgaact
    13501 ggtatccatt gaaaatctgc caggacgcat cgcggcaaac tcagttatcc cgtatccgcc
    13561 aggaatcccg atgctgctgt ctggtgaaaa cttcggcgat aaaaacagtc cgcaagtaag
    13621 ttatttacgc tcgctgcaat cctgggacca ccatttccct agatttgaac acgaaactga
    13681 agggactgaa attattgacg gtatttacca cgttatgtgc gtgaaagcgt aaccactatt
    13741 ccgctgaagg cgtaattgtt taaataacat tacgccgcct ggccttaggc cttttgagta
    13801 tggcaacgtt ttcataaaaa ttgctgcaaa caaaaatgtc atactttttg cgcggcccca
    13861 ccccacgctt ttttttgcct gttatttatc ctgtaaaaaa atatgtacat gagaaattac
    13921 tataaaaatt tgtaatatta gtaaaactcg ttatttttat gcatgtttat attcatcatg
    13981 caattatata accatttccc ggtatcgctt tgctttagcg agaactggtc ttttattcgc
    14041 actcaggagt acatgtatga ggatttgcag cgaccaacct tgtattgttt tattgactga
    14101 aaaagatgtc tggataaggg tgaatgggaa agaacctatt agccttaaag ctaaccatat
    14161 ggcgttatta aattgtgaaa ataatattat cgacgtctcc tctcttaaca acactttggt
    14221 tgctcatatt agtcacgaca tcatcaaaga ttacctccgg tttctgaata aagatctctc
    14281 gcaaatacca gtatggcaac gtagcgctac gcccatactc accctgccat gcctgacgcc
    14341 agacgtcttt cgcgttgccg cgcaacacag catgatgccc gcagaaactg agtcagaaaa
    14401 ggaacgaaca cgcgcattat tattcacggt gctatcccgt tttctcgaca gtaaaaaatt
    14461 cctttcatta atgatgtata tgttacgtaa ttgtgtaagt gacagcgttt atcaaattat
    14521 tgaaagcgat attcacaaag actggaatct tagtatggta gccagttgtt tatgtcttag
    14581 cccaagtctg ttaaagaaaa agttgaaaag cgaaaacacc agttatagcc aaataatcac
    14641 cacctgccgc atgcgttatg ccgtaaatga attaatgatg gacggtaaaa atatctctca
    14701 ggtatcacag tcctgcggct acaacagtac gtcgtacttt atttctgtct ttaaagactt
    14761 ctacggtatg acgccgctgc attatgttag tcagcacaga gaacgcactg tcgcctgatt
    14821 tttaacctta acgaagagct atattaataa cggcatcagc gataacccgg tcgataataa
    14881 ttcaactatc gaatgcaggc gtatgatatg acgtaattta ttgtcacgaa gctcgccttc
    14941 gcaggagttt aattatgtct tcggatgctg atgctcacaa agtgggctta atccccgtca
    15001 ccctgatggt gtcggggaat attatggggt caggtgtttt tctgttacct gcaaacctgg
    15061 cctccactgg cgggattgcc atttatggat ggttggtgac gattatcggt gcgctggggc
    15121 tctcgatggt atacgccaaa atgtcgttcc tcgacccaag tcctggtggt tcttacgctt
    15181 acgcccgccg ctgctttggc ccgtttctcg gttatcaaac caacgtcctc tactggctgg
    15241 cctgctggat cggcaatatc gccatggtgg tcattggcgt aggatattta agttacttct
    15301 tcccgattct gaaagatcca ttggtattaa ccatcacctg cgtcgtggtg ctgtggatct
    15361 tcgtcctgct gaacattgtc ggtccgaaaa tgatcacccg cgtgcaggcc gttgccactg
    15421 tgctggcgct gatccccatc gtcgggattg ccgtatttgg ctggttctgg ttccgtggtg
    15481 aaacctatat ggcggcatgg aacgtcagcg gcctgggcac cttcggtgcg attcaaagta
    15541 cccttaacgt tacgctgtgg tcgttcatcg gtgtggaaag tgcctccgtt gccgcaggtg
    15601 tggtgaaaaa cccgaaacgc aatgtcccta tcgccaccat tggtggggta ttgattgccg
    15661 ccgtttgcta tgtgctctcc accaccgcga ttatggggat gatccctaat gccgcactgc
    15721 gcgtttctgc ctcgccattc ggtgatgccg cacggatggc gttgggtgac accgctgggg
    15781 ccattgtttc cttctgcgca gctgcgggtt gcttaggttc actgggcggc tggacgttgc
    15841 tggcgggtca aacggcgaaa gccgctgccg atgacggact gttcccaccg atttttgccc
    15901 gtgtaaataa agcgggtacg ccagtggcgg ggctgattat cgtcggtatt ttgatgacca
    15961 tcttccagct cagcagcatt tcaccaaacg cgaccaaaga gttcggtctg gtttcttccg
    16021 tctcggtcat ctttacactg gtgccatatc tttacacctg tgcggcgtta ctgctgctcg
    16081 gccacggtca ctttggtaaa gcacgcccgg catatctggc tgttactacc attgccttcc
    16141 tctactgcat ttgggccgtg gtggggtccg gggcgaaaga ggttatgtgg tcatttgtca
    16201 ccctgatggt catcaccgcc atgtatgcgc tgaattacaa ccggctacat aaaaacccgt
    16261 atcccttaga tgcaccaata agcaaagatt aattcccctt aatccagcaa acataaaagc
    16321 caaccttaag aacttaaggt tggcttaatt ttgctttgcg agcatatgcg cactttgttc
    16381 gatggaaaca ccgtgatgtt gaagcgccta ctaaaaagac cctctttgaa tttactcgcc
    16441 tggctattgt tggccgcttt ttatatctct atctgcctga atattgcctt ttttaaacag
    16501 gtgttgcagg cgctgccgct ggattcgctg cataacgtac tggttttctt gtcgatgccg
    16561 gttgtcgctt tcagcgtgat taatattgtc ctgacactaa gctctttctt atggcttaat
    16621 cgaccactgg cctgcctgtt tattctgatt ggcgcggctg cacaatattt cataatgact
    16681 tacggcatcg tcatcgaccg ctcgatgatt gccaatatta ttgataccac tccagcagaa
    16741 agttatgcgc tgatgacacc gcaaatgtta ttaacgctgg gattcagcgg cgtgcttgct
    16801 gagctgattg cctgctggct aaaaatcaaa cctgccacct cgcgtctgcg cagtgttctt
    16861 ttccgtggag ccaatattct ggtttctgta ctgctgattt tgctggtcgc cgcactgttt
    16921 tataaagact acgcctcgtt gttccgcaat aacaaagagc tggtgaaatc cttaagcccc
    16981 tctaacagca ttgttgccag ctggtcatgg tactcccatc agcgactggc aaatctgccg
    17041 ctggtgcgaa ttggtgaaga cgcgcaccgc aacccgttaa tgcagaacga aaaacgtaaa
    17101 aatttgacca tcctgattgt cggcgaaacc tcgcgggcgg agaacttctc cctcaacggc
    17161 tacccgcgtg aaactaaccc gcggctggcg aaagataacg tggtctattt ccctaatacc
    17221 gcatcttgcg gcacggcaac ggcagtttca gtaccgtgca tgttctcgga tatgccgcgt
    17281 gagcactaca aagaagagct ggcacagcac caggaaggcg tgctggatat cattcagcga
    17341 gcgggcatca acgtgctgtg gaatgacaac gatggcggct gtaaaggtgc ctgcgaccgc
    17401 gtgcctcacc agaacgtcac cgcgctgaat ctgcctgatc agtgcatcaa cggcgaatgc
    17461 tatgacgaag tgctgttcca cgggctggaa gagtacatca ataacctgca gggtgatggc
    17521 gtgattgtct tacacaccat cggcagccac ggtccgacct attacaaccg ctatccgccg
    17581 cagttcagga aatttacccc aacctgcgac accaatgaga tccagacctg taccaaagag
    17641 caactggtga acacttacga caacacgctg gtttacgtcg actatattgt tgataaagcg
    17701 attaatctgc ttaaagaaca tcaggataaa tttaccacca gcctggttta tctttctgac
    17761 cacggtgaat cgttaggtga aaatggcatc tatctgcacg gtctgcctta tgccatcgcc
    17821 ccggatagcc aaaaacaggt gccaatgctg ctgtggctgt cggaggatta tcaaaagcgg
    17881 tatcaggttg accagaactg cctgcaaaaa caggcgcaaa cgcaacacta ttcacaagac
    17941 aatttattct ccacgctatt gggattaact ggcgttgaga cgaagtatta ccaggccgcg
    18001 gatgatattc tgcaaacttg caggagagtg agtaaatgaa aattctgatt gttgaagacg
    18061 atacgctgtt attgcaggga ctgattctgg cggcgcaaac cgaaggctac gcgtgcgatg
    18121 gcgtgacaac cgcgcggatg gcggaacaaa gccttgagga cggtcattac agcctggtgg
    18181 tactggattt agggttaccc gacgaagatg gactgcattt tctcgcccgt atccggcaga
    18241 aaaaatacac cctgccggta ctgatcctca ccgctcgcga tacgctgacc gacaaaatcg
    18301 ccgggctgga tgtcggtgcc gatgactatc tggtgaagcc ttttgcgctg gaagagttac
    18361 acgcccgtat ccgcgccctg ctacgacgcc ataataatca gggcgaaagt gagctgattg
    18421 ttggcaatct gacgctgaat atgggtcgcc gtcaggtatg gatgggcggt gaagagttga
    18481 ttctgacgcc caaagaatat gctctgctgt cacggttaat gctcaaagca ggcagtccgg
    18541 tacatcggga aattctctac aacgacatct ataactggga caatgaaccc tcgaccaaca
    18601 ccctggaagt gcatatccac aatctgcgcg acaaagtggg caaagcccgt atccgcaccg
    18661 tgcgcggctt tggctatatg ctggtcgcga atgaggaaaa ctaattgaat ctgatgcgtt
    18721 ttctgcgccg acctatatcg ctgcgccaac ggctgatatt gaccatcggg gccattttgt
    18781 tggtgtttga gctgatcagc gtcttctggc tatggcatga aagtaccgag cagattcagc
    18841 tgtttgagca ggcgctacgc gacaatcgca acaacgatcg ccacatcatg cgtgagatcc
    18901 gcgaggcggt cgccagcctg attgtccccg gcgtctttat ggtcagcctg acgctattta
    18961 tctgctatca ggcggtacgc cgcatcaccc gcccgctggc ggagctgcaa aaagagctgg
    19021 aagcgcgcac cgccgacaac ttaacgccca ttgccattca cagcgccacc ctcgaaatcg
    19081 aagcggtggt ttcggcgttg aacgatctgg tcagtcgcct gaccagcacg ctggataacg
    19141 aaaggttgtt taccgctgac gtcgcgcacg aactgcgaac gccactggcg ggggtgcgtt
    19201 tgcatctgga actgctggcg aaaacgcatc acattgatgt agcaccgtta gtggcacggc
    19261 ttgatcagat gatggagagc gtctcccagc tgctgcaact ggcgcgtgcc ggacagtcat
    19321 tttcttccgg taattatcaa catgtaaaac tgctggaaga tgtcattctc ccctcgtatg
    19381 acgaactcag taccatgctc gaccagcgac agcaaaccct gctattgcca gagagcgccg
    19441 ccgacatcac cgttcagggt gatgcaaccc tgctgcggat gttattgcga aacctggtag
    19501 aaaacgccca tcgttacagc ccgcaaggca gcaacattat gattaagctg caagaagatg
    19561 gcggagcggt catggcagtt gaagatgaag gaccaggtat tgatgagagt aaatgcgggg
    19621 agttgagtaa agcgtttgta cgtatggaca gccgttatgg cgggattggt ctggggttaa
    19681 gtattgtcag ccgcattaca cagttgcatc acgggcagtt tttcctgcaa aaccggcaag
    19741 agacttccgg cacgcgggcc tgggtacggc tgaagaaaga tcagaacgtg gcaaaccaga
    19801 tataaagaaa gctgctgacc accagcacgc tgaacacggt agttaaactt tcataaacac
    19861 ggtttttcat tgcactctcc tccgttaacc tggaggagag tatgcgcgtc agagattaag
    19921 tgaaccttaa gagttcaatt ggcctgataa gacagcgtca catcaggcca tccgtttcag
    19981 cttattcatc aattcgcgga tgttgctgca ccaggcgggt acgtttcgcc tgcaaatcgg
    20041 caatctcgtg gtcaatatta tcgattttct gctcgatatt atcgtaatgc tcgacgagaa
    20101 tttccttcgc ttcctgtata tctgacgccg ccggtgtcgc acctttcaac ggacgatttg
    20161 ccgtctcttt catggttacg ccggtgatta aaccaatcac cgccactacc atcaggtaat
    20221 aggcaggcat catcagattc tgcgagcttt cgaccagcca ggccgccagt gttggcgtca
    20281 gaccggcaac cagcaccgaa atattaaatg ccgccgccag cgcgctgtaa cggatatgcg
    20341 tcgggaacat cgctggcaag gtagaagcca taacgcccat aaagcagtta aggatcaccg
    20401 ccagcatcag taacccggca aaaatcaggc cgatgacgtt actgttaatc agaataaacg
    20461 ccgggatcgc caacacaaac agtgcaacac taccaagtag cacaaacgga cgacggccaa
    20521 aacggtcact cagcaagccc atcaccggct ggacaaacag cataccgatc ataatggcga
    20581 taataatcag caccccgtgg tcttcggagt aatgcaggtt atgcgacaaa taactcggca
    20641 tataggtcag caacatgtag taagtcacgt tggtggcaat taccagacca atacatgtca
    20701 acaggctgcg ccagtattta gtggcaatct ctttaaacga gactttcggg ccatcctgca
    20761 aaccttcgcg gtcgccctgt tccagtttat caacatgctg ctggaacgcc ggagtttctt
    20821 ccagcgcatg gcgcaggtaa agcccgataa tccctaacgg cagagcaata aagaacggaa
    20881 tacgccagcc ccagtcgagg aagttcgctt cgccgacaat ggtcgaaatt aacaccacca
    20941 cgcccgcacc cagcacaaac ccggcaatag aaccgaagtc cagccagctg cccataaagc
    21001 cacgtttacg gtcaggggag tattccgcaa caaatatcga cgccccggta tattcaccac
    21061 cgaccgagaa accttgtgcc atcttacaga tcaacagcag aatcggtgcc caaataccaa
    21121 tcgtgtcgta ggacggtatt aagccaatac agaacgtact gatcgacata atcacaatag
    21181 tgatagcgag gatcttctgg cgaccatatt tatcgcccaa cataccaaag aagagtccgc
    21241 caagcggtcg aatcagaaag ggaacggaga aagtggcaag tgcagcaacc atctgcacgc
    21301 tggggtcagc ccccgggaaa aaaactttac ctaatgcgta agcaacaaaa ccataaacac
    21361 caaaatcaaa ccattccatt gcattaccca gtgatgctgc ggtaatggct ttacgcagtt
    21421 taccgtcatc aataatggtg acatcacgaa gggtaatcgg ttttactttt ttccttttca
    21481 gcatagcttt cctcgcagag ttggcccatt tccgcaaacg ccagaaaaat cagcgcgacg
    21541 gccgcaaaag gggagcggga attgcaggat gcaatctctg taacaagcgt agcgggtttg
    21601 cttacaccct ccggttaagg aatgtacaat caaaccagtt gacgcctgaa ttgggcagtt
    21661 aatgaccttt ttaccctacc agtgtttaaa tttgtgatca acttcacaca tggatttcgt
    21721 ttaggactca ttgatgtaac tgattattta cgccaaattt acagagaaga tttcctcgac
    21781 cattgccccc accaaacgct gcgctgaaca gagtcgtggc atccccagcg cgacagtttg
    21841 ccagcgttgg gtcactgacc agcaaacggg ttgtttatct gccgtggtcc actttcccag
    21901 ccaacttaaa aaatcagcat gatccccgcc cgtcggaacg gtcgaagagg caaaccactg
    21961 atggtaatag tgacgcgtag tcggtgccgc attgatactc tctgccagat gttgtgtcgc
    22021 tttctcgcgc aaatcaccca gcaacaacac caccgcttgc gggcttccca gacggcaggc
    22081 atcgccaaat gcaccccagc gcaacccctc cagaacgata aaacagcgcc caggcaacga
    22141 ccaggcgtta tacgccccag cacgccagcg ggtaaaaatt tgctcgctct gatcacctgc
    22201 ctgcaccagt aggcctcgtt gcgttaacgc cttttccttc cagcgacaac gggctgaaaa
    22261 ccactcatac aaggcggcaa tttgctgggg gagctccggg aaagcagagt gctggcggtg
    22321 atccattttt tgcagcagcg ccagatgctg cgcgatctgc gtcagcgcca gatgaccggg
    22381 atccagcatc gacgccagtt tttccaccat tgtcagtcgc aggctaagcg gcgcatttaa
    22441 catgccgccg ttcgtccatg tctgaagctg cacacgcgaa agaagttcct gcatcagtcg
    22501 ttcacgaaac tgctgctgat gggcttgagg atggcgggca tcatcgccct gcgccagatc
    22561 cactaaaaag cgcggatgaa ccgactccag cacgcgaccg gggccgtcca gtaacgtctt
    22621 ggtcaatatc gttctgccgt gaaaagtgtt tgaatatcat cgcgtaacag ctgagcgtcg
    22681 gtgtaaatcc agctgtgagt catcacagtc tgctgcaatt gctgctgcat cagcctgacc
    22741 accgattcat tttgttgacg cagagccaga ctttcgcgta aacgcgtctg taattctgtc
    22801 aaacatgaag cgaactcagc gaaaaaagta ttcatgcctg ccgtaacaga ttcatcgacc
    22861 tgctctgcca gaactttagc catttgttgg caatagagat cgacttctgc gcttaatgct
    22921 cgttgcaaca cactgtaatc aaccgtttct gtcggggatt tctcatttcc ccgtccccag
    22981 tcgggctgat tcaaccagcg cgaaaaagtc tcacgcacaa cgcctaaacg cgtgctctgc
    23041 tcgtccgttg cttcctggcg cgaaatgact tcactgaaca gctggcgagt gttgaagtgg
    23101 ggaactacgc cgtgaaaaac aggaaaatga aacccaggac gaaaccctga ctcgctcaat
    23161 tccattttga cttgttgctc aatggggcga ataacatcgg ttaacacccg gcaaagggtg
    23221 gattccatct cggcaaaacg cagcgtaaag tcgcgactga tggtgttctg cgccgtctgt
    23281 aacagtgtct cacagcgggt acgaatctcg cttaacggct ccgaatcatc ctgaaacaag
    23341 gcggctaact gcgcattcac cgcatcttgc tgttgacgca gaaagtggtt ggcggaggtc
    23401 agggccagct cgatttcatg tttaatctcg ccgctcacct gcgcctgatt gagttgcaat
    23461 agctgcaaac tttcttcgac ctgatggata ttttgccgca attgttcaca agcgacgttt
    23521 aacccgtgcg cacgaaaatc caggtattcc cgcgcctgct gcgcgtaatt caacagttta
    23581 tgcgcagcag atcgcagagc atacaatgag gcgttagcgt aagcggcatg aagcaacgcc
    23641 tgaattggct gggcgaacag cgaatcttcc cacaactgat cggcagcatg acgaatatgt
    23701 tcgaggtccg ccagatcggc atgacgccag cgcctgccga gcgcggcatg ggcaaaatct
    23761 tccacccagc gttgttgctc tggcgctggt aacttaccgt tgttggctaa ctcatagcgc
    23821 gcccgattcg ccaggtagcc ccacatcgac gacaccggga atatctgctg tggcgtaata
    23881 cagcctttca tcagcgtccc ggaaatcagt gcccgcacct ggtcggcgtc gtcactgtta
    23941 cgatcctgtt gatcgaactt attgaccagc acatacagcg gcaccgattg ccccaccgcc
    24001 aaaatcgcct cacggacctc ttcatcggag atcgatttca gttgcgtata atccagcacc
    24061 gccagtaccg ccgaggcgcg tgccagctgc tggttaagca ttttttgcag atgcggttgc
    24121 ccggcttcat ttggcccggg ggtatccagt aacgtcaact gaccgggata actttccagc
    24181 cccgccagat ggacaaactc cacttcaatc acgggaatat gctcaatggc ggcgtaagca
    24241 gaaaaaggaa aatcgacgtc cagcgccttc gccagtcgca ctaaatcatt caaacttttc
    24301 agacaatgaa aaataggctg ggcacccaga taatattttt cgaaagcgac gccattttcg
    24361 atccgctgca taagcgcacg catatcttta tctatttcca gcacatcggt cagatgctta
    24421 atatcgcaat cacgcaggcg ctgttgtaat tgttgaatta aacaatcgat tggcgcgaca
    24481 tgtgaaaaat gcagtaccgg ttccttttgc ccgggcgtat ggcgaataag cgtcggcagc
    24541 gcagtcattg ggcgattacg attaggcaga acctccgtac caacaatggc attaatggtg
    24601 gttgatttcc ctgctttcat ggtaccgaca attgccagca ccatttccag tcgggaaatt
    24661 ttacacaact cattattcag catcgcgtga cgttcggcga tattaggctg actccagggt
    24721 aaagccagtt gtggcgcgtc gtctccgggt acagagagag gcattttttc cagtaactgc
    24781 aactgttggc gagaaagctg taacaggcgt tcagcctcct gacttaactc atacagggtc
    24841 tgtgtgtaca tagaaaattc ttccttaaag caaattttgt tacttttatt tagccagatt
    24901 gtttttgagt tctgttttcg gcttttataa ttactgcaag aaataatttt atatttagtg
    24961 tgttgttttt ttatcagaat aaataacgtc ttctgatgcg tttaaaacgt cagaaagata
    25021 aaaatatcat gtgaataaaa aaaagaacaa gtagagcatt aacattatct taaataataa
    25081 atagaggcaa aaagattatt ttctttttgc gtttcctttc aaatgaaaac gatcgtcgtc
    25141 taaaatcagc agtacccccg acaaactcag ggattttgtg tataattgcg gcctttttcg
    25201 gcaatctgcc gttttttggc gcttttgccc tgctgacttt tgaggaaatc cacatgtcat
    25261 taccacactg cccaaaatgc aactccgaat acacttacga agataacggc atgtacatct
    25321 gcccggaatg tgcctacgaa tggaacgacg cagaacctgc acaggaaagc gacgagctga
    25381 tcgttaaaga tgctaacggc aatctgctgg ctgacggcga cagcgttacc atcattaaag
    25441 atctgaaggt gaaaggtagc tcttcgatgc tgaaaattgg caccaaagtg aaaaacatcc
    25501 gcctggttga aggcgaccat aacatcgatt gcaaaatcga cggttttggt ccgatgaaac
    25561 tgaaatctga gtttgtgaaa aagaactgat tgtattgtga tcggtaagcc ggataaggcg
    25621 ctcgcgccgc atccggcaac gatgccgact gcctgatgcg acgcttgcgc gtcttatcag
    25681 gcagcctctc atctgtataa atttcgaact acacttaact ggcttctctt aactgaggtc
    25741 accatcatgc cgttaagtcc ctacctctct tttgccggta actgttccga cgcgattgcc
    25801 tattatcaac gtacgttggg cgcggaactg ctctataaaa tcagcttcgg cgaaatgccg
    25861 aaatcagcgc aggacagcgc cgagaactgc ccttccggaa tgcaatttcc cgataccgcc
    25921 atcgctcatg ccaacgtgcg cattgccgga agcgacatca tgatgagcga tgctataccg
    25981 tcaggaaaag ccagctactc cggctttacg ctggtgctcg attcgcaaca ggtcgaagaa
    26041 ggaaaacgct ggtttgataa ccttgccgct aacggaaaaa tcgaaatggc ctggcaggaa
    26101 actttctggg cgcatggctt tggcaaagtc accgataaat ttggcgtacc gtggatgatt
    26161 aatgtcgtca aacaacaacc aacgcaataa cccgccggga ggcccgccct cccgcactgt
    26221 catcgaattc ccgttaactc ttcatctgtt agtcactttt aattaaccaa atcgtcacaa
    26281 taatccgcca cgatggagcc acttttttag ggaggctgca tcatgcaaac gattatccgt
    26341 gtcgagaagc tcgccaaaac cttcaatcag catcaagcgc tgcatgcggt tgatctgaac
    26401 attcatcaca gtgaaatggt ggctctgctt gggccgtcgg gttccggcaa atccaccctt
    26461 ttacgtcatt taagcggttt gattaccggc gataaatccg ccggtagcca tatcgagcta
    26521 ctgggccgca ctgtccagcg cgaaggccgc ctggcccgcg atatccgcaa aagccgcgcc
    26581 aacaccggct acatctttca acaattcaac ctggtgaacc gcctgagcgt actggagaac
    26641 gtgctgattg gcgcgctcgg cagcacgccg ttctggcgca cctgttttag ctggttcacc
    26701 ggcgagcaga aacagcgcgc gttacaggcg ctgacccgcg ttggcatggt gcattttgcc
    26761 catcagcgcg tttccaccct ctccggcggc cagcagcaac gtgtggcgat tgcccgtgcg
    26821 ctgatgcagc aggcgaaagt gattctggcc gatgaaccca tcgcctcgct ggacccggaa
    26881 tcagcgcgca tcgtgatgga caccctgcgc gacatcaacc agaacgacgg catcaccgtg
    26941 gtcgtcacgc tgcatcaggt ggattacgcc ctgcgctact gcgaacgcat cgtcgccctg
    27001 cgtcaggggc acgtcttcta cgacggcagc agccaactgt ttgataacga acgttttgac
    27061 catctctacc gcagcattaa ccgcatcgaa gagaacgcga aagctgcctg atatccccat
    27121 cattgaggaa aacgaatgaa cgctaagata attgcctcgc tggccttcac cagcatgttc
    27181 agcctcagca ccctgttaac cccggcacac gccgaagagc aggaaaaggc gctgaatttc
    27241 ggcattattt caacggaatc acagcaaaac ctgaaaccgc aatggacgcc gtttttacag
    27301 gatatggaga agaaactggg cgtgaaggtc aacgccttct ttgccccgga ctacgcgggc
    27361 attatccagg ggatgcgctt caataaagtg gatattgcct ggtacggcaa cctgtcggcg
    27421 atggaagcgg tggatcgcgc caatggccag gtcttcgccc agacggtcgc ggcggatgga
    27481 tcgccgggtt actggagcgt gttgatcgtc aacaaagaca gcccgatcaa caacctgaac
    27541 gatctgctgg cgaagcggaa agatctcacc ttcggcaatg gcgatcctaa ctccacctct
    27601 ggcttcctcg tccccggtta ctacgtcttc gctaaaaaca atatctccgc cagcgacttc
    27661 aagcgcaccg tcaacgccgg gcatgaaacc aacgcgctgg ccgtcgccaa caagcaagtg
    27721 gatgttgcca ccaacaacac cgaaaacctc gacaagctga aaacctccgc gccggagaag
    27781 cttaaagagc tgaaggtgat ctggaaatcg ccgctgatcc taggcgatcc gatcgtctgg
    27841 cgcaagaatc tgtccgaaac taccaaagac aagatctacg acttctttat gaactacgga
    27901 aaaacgccgg aagagaaagc ggtgctggaa cgcctgggct gggcgccgtt ccgcgcttcc
    27961 agcgacctgc aactggtgcc gattcgccag ctcgcactgt ttaaagagat acaaagcgtg
    28021 aaaggcaata aagggctgaa tgagcaggac aagctggcga aaaccaccga gattcaggcg
    28081 cagctggatg acctggaccg cctgaacaac gcgttaagcg cgatgagttc ggtgagtaaa
    28141 gcggtgcagt aaatcgtagg tcggataaga cgccccggcg tcgcatccga caatgtgtag
    28201 gcgttgatgc cggatgcggt gcaagcacct tatccggcct acagaccgga gccaaacatg
    28261 caaaccatca ccatcgcccc acccaagcgt agctggttct cgcttctgag ctgggccgtt
    28321 gttctcgacg tgctggtcgt ctcgtggcag ggcgcggaaa tggccccgct tacgctgatt
    28381 aaagacggcg gcaacatggc aaccttc
//
LOCUS       DAETQL010000053        28365 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.053, whole genome shotgun sequence.
ACCESSION   DAETQL010000053 DAETQL010000000
VERSION     DAETQL010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.053"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="K0W65_002454"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="K0W65_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(1023..2045)
                     /locus_tag="K0W65_002455"
     CDS             complement(1023..2045)
                     /locus_tag="K0W65_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3095684.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /locus_tag="K0W65_002456"
     CDS             complement(2059..3561)
                     /locus_tag="K0W65_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBI3095685.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="K0W65_002457"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="K0W65_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="K0W65_002458"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="K0W65_002458"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBI3095686.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="K0W65_002459"
     CDS             complement(5877..6218)
                     /locus_tag="K0W65_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBI3095687.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="K0W65_002460"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="K0W65_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBI3095688.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="K0W65_002461"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="K0W65_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBI3095689.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="K0W65_002462"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="K0W65_002462"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBI3095690.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="K0W65_002463"
     CDS             12897..14240
                     /locus_tag="K0W65_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBI3095691.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="K0W65_002464"
     CDS             complement(14302..14508)
                     /locus_tag="K0W65_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBI3095692.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="K0W65_002465"
     CDS             14833..15387
                     /locus_tag="K0W65_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBI3095693.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..16388)
                     /locus_tag="K0W65_002466"
     CDS             complement(15450..16388)
                     /locus_tag="K0W65_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095694.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="K0W65_002467"
     CDS             complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="K0W65_002467"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBI3095695.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17530..19473
                     /gene="cpdB"
                     /locus_tag="K0W65_002468"
     CDS             17530..19473
                     /gene="cpdB"
                     /locus_tag="K0W65_002468"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBI3095696.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19599..19979)
                     /locus_tag="K0W65_002469"
     CDS             complement(19599..19979)
                     /locus_tag="K0W65_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBI3095697.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20068..20928
                     /gene="qorB"
                     /locus_tag="K0W65_002470"
     CDS             20068..20928
                     /gene="qorB"
                     /locus_tag="K0W65_002470"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBI3095698.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21037..22002
                     /locus_tag="K0W65_002471"
     CDS             21037..22002
                     /locus_tag="K0W65_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI3095699.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22110..22772
                     /gene="ytfE"
                     /locus_tag="K0W65_002472"
     CDS             22110..22772
                     /gene="ytfE"
                     /locus_tag="K0W65_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBI3095700.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="K0W65_002473"
                     /gene_synonym="dagA"
     CDS             complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="K0W65_002473"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBI3095701.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="K0W65_002474"
     CDS             complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="K0W65_002474"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBI3095702.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25376..25921
                     /locus_tag="K0W65_002475"
     CDS             25376..25921
                     /locus_tag="K0W65_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HBI3095703.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25905..26552)
                     /locus_tag="K0W65_002476"
     CDS             complement(25905..26552)
                     /locus_tag="K0W65_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBI3095704.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26764..27555
                     /locus_tag="K0W65_002477"
     CDS             26764..27555
                     /locus_tag="K0W65_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HBI3095705.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27565..>28365
                     /locus_tag="K0W65_002478"
     CDS             27565..>28365
                     /locus_tag="K0W65_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBI3095706.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINS"
BASE COUNT         6960 a         7551 c         6992 g         6862 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aattgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatcttttt tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt
    16321 gtatgcgatg actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg
    16381 ggtaacatat ggctacctta ttttatttgg ttattttatg tcgccattaa atatctcatt
    16441 atagaagtaa actatcaaaa aacgctcatt attaaaatgt aacttactag caattgaatt
    16501 tttgcacttt aaatacaatc tctttttatt tataactttt taggttggga taaatcagga
    16561 tggcagaatc aggaaataca ctgtttctgc catctgaatt tagtaaatgg acactctgaa
    16621 ccccggattc aggaacgact cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg
    16681 aacgtgcgct ccggcagctg cagccacagc atgtccggcg gcggtgtccc aaatattcgt
    16741 tggtccgaag cgcgggtaca gctgcgcctg tccttccgcc accaggcaga atttcagcga
    16801 agagccgatg gacgtggtct gatgttcgcc aagctgttgc agatactctt tcagctccgc
    16861 atctgcatgg gaacggctga taaccaccag cggcgggcgc gcatcgcgga cctgaatcag
    16921 cttgcgcaca ccgcactctt ctttccaggc cttgccttct gccgcgctgt acattacgtt
    16981 cattaccggc gcatacacca cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt
    17041 aacggtgaat tcgccattac gtttaataaa ctctttagta ccatccagcg ggtctaccag
    17101 ccagtaacgc tgccagtgct gacggacttc ccaaccggga ggatcttctt cagaaaggac
    17161 cggaatatcc ggtgccagcg tacgtaaacc gtccatgata acggtgtgag cggcaatatc
    17221 cgctgccgtt accggagaat tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc
    17281 gtagacctgc ataatggcat cgcctgcatt ccgtgcaagc tggcatactt gatctaacat
    17341 ttctccacct cgtctctgtg agcggtgtta acttattgtt ttacttatac cctatcgtta
    17401 atgaatgcgc caactgtgat agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc
    17461 actgttctat aagtaaggcg tttattctcc cttttctttc gtattcccga tgataaaagg
    17521 atgtccctga tgattaagtt tagcgcaacg ctcctggcca cgctgattgc cgccagtgtg
    17581 aatgcagcga cggtcgatct gcgtatcatg gaaaccactg atctgcatag caacatgatg
    17641 gatttcgatt attacaaaga caccgccacg gaaaaattcg gactggtacg tacggcaagc
    17701 ctgattaacg atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg
    17761 attcagggga gtccgctggc cgattacatg tcggcgaaag gattaaaagc aggtgatgtt
    17821 catccggttt ataaggcgct gaatacgctg gattatacgg tcggtacact cggcaatcat
    17881 gaatttaact acggtctgga ttacctgaaa aatgcgctgg cgggagcgaa attcccttat
    17941 gtaaatgcca acgtcattga cgccagaacc aaacagccaa tgtttacacc gtatttaatt
    18001 aaagataccg aagtggtcga taaagacgga aaaaaacaga cgctgaagat tggctatatt
    18061 ggcgtcgtgc cgccgcaaat catgggctgg gataaagcta atttatccgg aaaagtgacg
    18121 gtgaatgata ttaccgaaac cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc
    18181 gatgtcattg tcgttctggc gcattccggg ctgtctgccg atccgtataa agtaatggcg
    18241 gaaaactcag tttattacct cagtgaaatt ccgggcgtta acgccattat gtttggccat
    18301 gctcacgccg ttttcccggg taaagatttt gctgatatcg aaggggctga tatcaccaaa
    18361 ggcacgctga atggtgttcc ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg
    18421 gtcgacttac aactcagtaa tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa
    18481 gcacggccga tttacgacat cgccaataaa aaatccctcg cggcggaaga cagcaagctg
    18541 gtagaaacac tcaaagccga tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt
    18601 aaatctgccg acaatatgta tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa
    18661 gtcgtgaaca acgcgcaaaa agcgtatgtc gaacattaca ttcagggcga tccggatctg
    18721 gcaaaactgc cggtgctttc agctgccgca ccgtttaaag ttggtggtcg caaaaatgac
    18781 ccggcaagct atgtggaggt ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt
    18841 tatctctacc ccaatacgct gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg
    18901 ctggagtgct ccgccggaca gtttaaccag attgctccta acagcacgaa accacagtca
    18961 ctcatcaact gggatggttt ccgcacttat aactttgatg ttattgatgg tgtgaattat
    19021 cagattgatg ttacccaacc cgcccgctat gacggcgagt gccagatgat taatgccaat
    19081 gcagagcgga ttaagaacct gacctttaat ggcaagccga ttgatccgaa cgctatgttc
    19141 ctggttgcca ccaataacta tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc
    19201 catatcgctt ttgcttcacc ggatgagaac cgctcggtgc tggcagcgtg gattgctgat
    19261 gagtcgaaac gtgcggggga aattcatccg gcggcagata acaactggcg tttagcaccg
    19321 atagctgccg ataagaaact ggatatccgt ttcgaaactt ccccgtcaga taaagccgca
    19381 gcgtttatta aagagaaagg gcagtatccg atgaataaag tcgcgaccga tgatatcggg
    19441 tttgcgattt atcaggtgga tttgagtaag taaaacactt ctttttcggc ctataaatca
    19501 accgcatccg gcatttattg gcggatgcga tgctgccgta tcttatccac cctacaagcc
    19561 atgaaccgta gaccggataa gcgcagcgca tccggcagtt atgccgcacg ttcatcccgc
    19621 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    19681 ctcacctgct cgcccagcgg cgtgaggcta tattccacat gcggcggcac caccggatac
    19741 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    19801 tcactcaccc caccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    19861 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    19921 cactgttccg caaagagatt accctctttc agttgttgtg acaggctaac ctgactcatt
    19981 tcatacttac ctttttgcac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    20041 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    20101 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    20161 gttcgtaatc cggcaaaagc ccaggccctg gcagcacaag gcattaccgt gcgtcaggct
    20221 gactacagcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    20281 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    20341 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    20401 ctcggcctcg ccgatgagca catcgagacg gagaaaatgc tggctgattc tggcatcgtt
    20461 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    20521 ctggaacacg gcgtatttat cggtgcggct ggcgatggca aaatcgcctc agcaacgcgg
    20581 gcagattatg cgacagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    20641 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    20701 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    20761 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    20821 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ctgcccaacg
    20881 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    20941 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    21001 tgaacgtttc ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc
    21061 ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt gccggaatac
    21121 ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctacccatt
    21181 gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg
    21241 atgctcacta tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt
    21301 actggcgcgc ctgtttccac gatgattatc ggcaccctgc cggtggtgat tcctgtcttt
    21361 gccaatctgc tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca
    21421 ctgatttgta ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc
    21481 cccgattttg actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc
    21541 tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat
    21601 ccgatgatgt gggcgacggc gcaggcgctg gtcacgctgc cggtttctct catcggctat
    21661 ctcgtcgcct gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc
    21721 cgtccgctgg tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc
    21781 gcactctgct ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt
    21841 gttttcgaaa cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg
    21901 ctaatgacgc tgagcggtat cgcgctgtta gtgattggcg tggtcatcgc ggtcagagca
    21961 aaaccagaaa aacctttaac tgaatctgtc tcagaaagtt gacatgctgg cagtgagtta
    22021 aataagcctc tgctacgtaa gggttatagc ttttgcctta aaggtgtatt taaaatacat
    22081 cttatcttat taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac
    22141 tggcgctctc tatccctcgc gcttcggctc tgtttcgtaa atatgatatg gattactgct
    22201 gtggcggtaa gcagacgctg gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca
    22261 ttgaagctga actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc
    22321 cgctggcaga aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcagc
    22381 tgccggagct gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg
    22441 tgccaaaagg gctgacaaaa tacctgacca tgctgcatga agagctttcc agccacatga
    22501 tgaaagaaga gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg
    22561 ggccaatcag cgtaatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatca
    22621 aacacactac caataacgtc acaccgccgc cagaagcctg caccacctgg aaagcgatgt
    22681 ataacggcat taatgaactg attgatgacc tgatggatca catcagtctg gaaaacaatg
    22741 tactgttccc acgcgcccta gcgggtgagt gatctaaaag ctggatggca ttgcgccatc
    22801 cagcatgata atgcggttat ttccgcagtt cagcagcccg cttcttacca ataaacagcc
    22861 agcccaaccc cagcgcgata aaccacagcg gggtaaccag cagcgcctgg cgagtgtcat
    22921 cttccagtgt cagcaacacg accacgaaca caaagaacgc catacatacc cagcacatca
    22981 gcttgccgag cggcatcttg tagatcgact tttcatgcag atgaggacgc tgtttgcggt
    23041 acaccaggta agagcaaagg ataatcgtcc agacgaacat aaacagaatc gcggaaacgg
    23101 ttgtaatcat cgtgaacgcg ccaatcacgc taggattcac atacaacatc accacgccgc
    23161 cgagcagaca gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt
    23221 tagcgaacgc tttcggcgca acaccttcct gcgccagacc aaacagcata cggctggtag
    23281 agaagacacc gctgttagcg gaagacgccg cagaggtcag caccacaaag ttgatcacgc
    23341 tggcagccgc aggcaaacct accaacacaa acagctcaac gaacgggctt ttctccggga
    23401 ctaccgaact ccacggtgtc acggacataa tcacaatcag cgcgaagacg tagaacataa
    23461 tgatacggat cggaatggag ttaatcgcgc gtggcagtga tttctccgga tctttggttt
    23521 ccgcagctgt tgtacctacc agctcaatcc ccacgaaagc gaaaaccgct atctggaatc
    23581 cggcaaagaa gccacttaaa cctttcggga accagccgcc gtcattccac aaatgcgcga
    23641 atgacgcttc cacaccggtc ggtgactgaa agtgcatcgc caccatgacc aggccgacga
    23701 caatcagcga cacgatggcg acgattttga tcatcgcaaa ccagaactcc atctcaccga
    23761 acattttcac ggtggcaagg ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga
    23821 cccagtcgga gagatcgggg aaccagaact gagcataagc cgtgatcgcc accacgtctg
    23881 ccataccggt tacaacccag cagaaccagt aagtccagcc ggtgaaatat cccgcccacg
    23941 gccccagtaa atcggaagcg aagtcactaa aagatttgta ttccagattc gaaagcagca
    24001 attcccccat tgcccgcatc acgaaaaaga gcataaaacc aatgatcata taaacgaaaa
    24061 tgatcgacgg cccggcaagg ctaatcgttt tgccggaccc cataaacaac ccagtaccaa
    24121 tggcacctcc aatggcaata agctgaacat gtcggtttgt gagattgcgc cgtagcgact
    24181 gttcagccgg agcctgatca tcggcaacga cttttacctg atctaccatg ttttttcttc
    24241 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    24301 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    24361 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    24421 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    24481 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    24541 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcacct
    24601 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    24661 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    24721 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    24781 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    24841 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    24901 ttcacgcctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    24961 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    25021 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    25081 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    25141 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    25201 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    25261 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    25321 gttagcttat tcataggcta tgattgagga acaagacgtg gagcaggagg aaaccatgcc
    25381 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    25441 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    25501 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgtcggtgg tgacgcgtga
    25561 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc cgcaaactac
    25621 ccaaacgcaa ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt
    25681 cgaacccggt aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt
    25741 ctatgcaatg gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca
    25801 gatggtgaaa atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg
    25861 gaataatcaa caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta
    25921 gatgaagggt caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct
    25981 tccagtgcct ggggggaaac catactgata tgataaacta ggccaagcgt atgctggttg
    26041 atcaaataat atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatacct
    26101 ggcttaaaaa tattgtattt cttcccttca tccaataagg attccattaa ctgcaagtga
    26161 gcatggttaa tttctaagag acgctgtgat tttgcataat gcacaccttt actttgattt
    26221 tcactatgca caatttttaa aaaccaagga ttagcaatat aataatccca tgtaaaatca
    26281 ataattttct caatggcttt ttgtggcgga agatctgaga aatcaagggc cttttctttt
    26341 tgtcgaatat cgttccaggt atattccagc acttcgataa ataaattttc tttattacca
    26401 aagtggtgat aaaccgtctg tttactacac cctgctgcct ttacaatatt atctactctg
    26461 gccccttcat aaccatattc ggcaaattca ttaaccgcac atagcagcaa tttttccttc
    26521 aggctctgta cgttgctggt atcaatatac ataacgcttc cccacgacct cttcgtggtg
    26581 actatcataa cctgatttga tttcagcttc agaaaaaagg gcaaaaactt gtactttcat
    26641 caggttatca aatgtcctgt gatcgccatc gcatatcaac caactagatg gtttatcttt
    26701 ttatcctcca gtcgcaagct aatcatcacc aaggaaacaa aattcaaaaa agagagctat
    26761 tgtatgaaat ccactcgtcc tgttgccgtg attaccggag ccactcgcgg tattggaaag
    26821 ggctgtgcgc aggagcttgc tcgtggcggg tttaacctgc tgattaacga tcgtcccgat
    26881 gccgacagcg tagaaaaact gcatatcaca caacaagaat gtttggcgga aggcgtggaa
    26941 gtgatttgct tccctgctga tgtcggtgat ctatctctgc atgaggagat gctcgacgcg
    27001 gcgcaaaacc agtgggggcg gctggactgt ctgctcaata acgcggggat ttcagtgaaa
    27061 aaacgcggtg acctcctcga cctcgaacca gacagcttcg atcaaaacat tgccattaac
    27121 acccgcgcgc cgttcttcct tgctcaggca ttcagtaagc gcttactggc acagccgaaa
    27181 ccggaagcgg agctgccgca ccgctcgatt attttcgtca gttccatcaa cgccatcatg
    27241 ctggcgatga atcgcggcga atacaccatt gccaaaaccg ccgtttctgc cgctgctcgt
    27301 ctgtttgctg ctcgcctttg taatgaacaa attggcgttt atgaagtgcg ccctggtctc
    27361 atcaaaactg atatgactat tccggcaacg gcttattacg acgaactgat tgccaaaggt
    27421 ctggtgccgt ggggacgttg gggttatccg gctgatattg cttccaccgt ccgcgcgatg
    27481 gcggaaggta aactgattta cacctgtggt caggcggtcg caatcgacgg cggcttaagt
    27541 atgccgcgct tctgaggctg aatcatgaga gatctgttac aacgcccgga tttattcagt
    27601 atcaataccg cgacactggg ctataaaacg ccgctacccg ccattattga tgcctgcgcc
    27661 gcacgcggta ttggcgcaat tgccccctgg cgcagggagt tgcagggtga agatctccag
    27721 caaatcaccc gccagcttgc cgccagtaac atgagcgttt ccggtttgtg ccgtagcact
    27781 tactacaccg cgccgacact ggcggaacgc aaactggcga ttgatgataa ccgccgggcg
    27841 ctggacgacg cagcggtgct taacgccgcc tgttatatgc aggtggttgg cggtctgcca
    27901 acgggcacta aagatctata tgaagcgcga gaacaagtga aacaagggat tcgccagttg
    27961 ctgccacatt cgaaagatgt cggcgtgcca attgcgctgg agccactgca cccgatgaca
    28021 gctgccgatc gctcttgcct gtgcacgtta cgtcaggcgc tggactggtg tgatgaactc
    28081 gatcccgatg gtgagtttgg cctcggcgtg gcggtggatg tttatcacgt ctggtgggat
    28141 ccggatctcg ccagtcagat cctgcgtgcc ggaaaacgca tcctcgcgtt tcatgtttcc
    28201 gactggttaa tgccgaccac tgatctggtc aatgaccgtg gtatgccggg agatggcgtc
    28261 atcaatattc cgtcaattcg tcggctggtt gaaaacgccg gatttaatgg tgctattgaa
    28321 ctggaaatat tctcgcctta ctggtggcag aaagatatta acagc
//
LOCUS       DAETQL010000054        27601 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.054, whole genome shotgun sequence.
ACCESSION   DAETQL010000054 DAETQL010000000
VERSION     DAETQL010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.054"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="K0W65_002479"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="K0W65_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBI3095707.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="K0W65_002480"
     CDS             complement(1614..1937)
                     /locus_tag="K0W65_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBI3095708.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="K0W65_002481"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="K0W65_002481"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBI3095709.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="K0W65_002482"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="K0W65_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBI3095710.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="K0W65_002483"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="K0W65_002483"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HBI3095711.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="K0W65_002484"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="K0W65_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBI3095712.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="K0W65_002485"
     CDS             7537..8574
                     /locus_tag="K0W65_002485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBI3095713.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENSCQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="K0W65_002486"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="K0W65_002486"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBI3095714.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="K0W65_002487"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="K0W65_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBI3095715.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="K0W65_002488"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="K0W65_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBI3095716.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="K0W65_002489"
     CDS             10745..11626
                     /locus_tag="K0W65_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3095717.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="K0W65_002490"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="K0W65_002490"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBI3095718.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELHPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="K0W65_002491"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="K0W65_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBI3095719.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="K0W65_002492"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="K0W65_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="K0W65_002493"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="K0W65_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBI3095720.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="K0W65_002494"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="K0W65_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBI3095721.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="K0W65_002495"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="K0W65_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBI3095722.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="K0W65_002496"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="K0W65_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBI3095723.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="K0W65_002497"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="K0W65_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBI3095724.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="K0W65_002498"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="K0W65_002498"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBI3095725.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="K0W65_002499"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="K0W65_002499"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBI3095726.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="K0W65_002500"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="K0W65_002500"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBI3095727.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="K0W65_002501"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="K0W65_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBI3095728.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="K0W65_002502"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="K0W65_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBI3095729.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="K0W65_002503"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="K0W65_002503"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBI3095730.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="K0W65_002504"
     CDS             24893..25273
                     /locus_tag="K0W65_002504"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBI3095731.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="K0W65_002505"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="K0W65_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBI3095732.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="K0W65_002506"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="K0W65_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBI3095733.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27538
                     /locus_tag="K0W65_002507"
                     /pseudo
     CDS             26442..27538
                     /locus_tag="K0W65_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6808 a         6863 c         7235 g         6695 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaactc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggtgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaactgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgtaaaatg
    27541 aggtgtactg gcaatagcgg acactaccat ttgttctttt tttaagcagc catctgatga
    27601 t
//
LOCUS       DAETQL010000055        26565 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.055, whole genome shotgun sequence.
ACCESSION   DAETQL010000055 DAETQL010000000
VERSION     DAETQL010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26565
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.055"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            348..641
                     /locus_tag="K0W65_002508"
     CDS             348..641
                     /locus_tag="K0W65_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HBI3095734.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            713..1372
                     /locus_tag="K0W65_002509"
     CDS             713..1372
                     /locus_tag="K0W65_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBI3095735.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1464..1910
                     /locus_tag="K0W65_002510"
     CDS             1464..1910
                     /locus_tag="K0W65_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBI3095736.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="K0W65_002511"
                     /pseudo
     CDS             complement(2117..3017)
                     /gene="hlyE"
                     /locus_tag="K0W65_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3390..3809
                     /locus_tag="K0W65_002512"
     CDS             3390..3809
                     /locus_tag="K0W65_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBI3095737.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3809..5077
                     /gene="umuC"
                     /locus_tag="K0W65_002513"
     CDS             3809..5077
                     /gene="umuC"
                     /locus_tag="K0W65_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBI3095738.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="K0W65_002514"
     CDS             complement(5123..5653)
                     /gene="dsbB"
                     /locus_tag="K0W65_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBI3095739.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="K0W65_002515"
     CDS             complement(5799..7340)
                     /gene="nhaB"
                     /locus_tag="K0W65_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBI3095740.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7561..8280
                     /gene="fadR"
                     /locus_tag="K0W65_002516"
     CDS             7561..8280
                     /gene="fadR"
                     /locus_tag="K0W65_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBI3095741.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8332..9865)
                     /locus_tag="K0W65_002517"
                     /pseudo
     CDS             complement(8332..9865)
                     /locus_tag="K0W65_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
     gene            10195..11493
                     /gene="dadA"
                     /locus_tag="K0W65_002518"
     CDS             10195..11493
                     /gene="dadA"
                     /locus_tag="K0W65_002518"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBI3095742.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11503..12573
                     /gene="dadX"
                     /locus_tag="K0W65_002519"
     CDS             11503..12573
                     /gene="dadX"
                     /locus_tag="K0W65_002519"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HBI3095743.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12741..14155)
                     /locus_tag="K0W65_002520"
                     /pseudo
     CDS             complement(12741..14155)
                     /locus_tag="K0W65_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14766..16502)
                     /locus_tag="K0W65_002521"
     CDS             complement(14766..16502)
                     /locus_tag="K0W65_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBI3095744.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16597..17511)
                     /gene="ldcA"
                     /locus_tag="K0W65_002522"
     CDS             complement(16597..17511)
                     /gene="ldcA"
                     /locus_tag="K0W65_002522"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBI3095745.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17611..18222
                     /gene="emtA"
                     /locus_tag="K0W65_002523"
     CDS             17611..18222
                     /gene="emtA"
                     /locus_tag="K0W65_002523"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBI3095746.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18224..18958)
                     /locus_tag="K0W65_002524"
     CDS             complement(18224..18958)
                     /locus_tag="K0W65_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBI3095747.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19159..19413
                     /locus_tag="K0W65_002525"
     CDS             19159..19413
                     /locus_tag="K0W65_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBI3095748.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19463..20923)
                     /locus_tag="K0W65_002526"
     CDS             complement(19463..20923)
                     /locus_tag="K0W65_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBI3095749.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20860..21417)
                     /locus_tag="K0W65_002527"
     CDS             complement(20860..21417)
                     /locus_tag="K0W65_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HBI3095750.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21443..22297)
                     /locus_tag="K0W65_002528"
     CDS             complement(21443..22297)
                     /locus_tag="K0W65_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HBI3095751.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22297..23106)
                     /locus_tag="K0W65_002529"
     CDS             complement(22297..23106)
                     /locus_tag="K0W65_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBI3095752.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23116..23874)
                     /locus_tag="K0W65_002530"
     CDS             complement(23116..23874)
                     /locus_tag="K0W65_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3095753.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23871..24851)
                     /locus_tag="K0W65_002531"
     CDS             complement(23871..24851)
                     /locus_tag="K0W65_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBI3095754.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24851..25873)
                     /locus_tag="K0W65_002532"
     CDS             complement(24851..25873)
                     /locus_tag="K0W65_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HBI3095755.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26223..>26565
                     /locus_tag="K0W65_002533"
     CDS             26223..>26565
                     /locus_tag="K0W65_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095756.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSNDNIL"
BASE COUNT         6674 a         6472 c         6856 g         6563 t
ORIGIN      
        1 tatggccttt gctgaactca acattgacat tcttacccgc agcaaagctc gtggaggtta
       61 gcagtgcaag cagcaataca gccttactga tgctcttgat tttcattggt agtctcctgt
      121 tcagaatgag tacgatgaac tgttataata taacaatccc ttacgggaga attccagtgg
      181 cttttctgcg ttcggtaaaa tcccggcgcg gcattttttg ccggggggat tacttataaa
      241 ggggcatgga ctcaacaaac cgcagaaatc tacagctggc tggcttgtgg cgcgggtttc
      301 attgatgtta gcttatgcca aaacctggca tcttaaagag taaaagtatg ttttgtgcga
      361 tttatcgaag cagcaagcgt gaccagacct atttatatgt cgaaaaaaaa gacgattttt
      421 cgcgtgttcc tgaggaactg atgaaaggtt ttggtcagcc tcagttagcg atgattctgc
      481 cgttggatgg gcggaagaaa ctggtcaatg ctgatattga aaaggttaaa ctggcattaa
      541 ccgagcaagg ttactatttg cagttaccgc caccacccga agatttgctg aagcaacatc
      601 tttccgtcat ggggcagaaa acagacgaca ctaacaaata accgatatcc ggcggtggca
      661 ttatctttgt cggcgcgggt tttcatatcc acgataaggt gaggggaacg ttatgtatca
      721 acatcacaac tggcaaggtg cgctgctgga ttatccggtg agtaaagtag tctgtgttgg
      781 cagtaactat gccaaacata ttaaagagat gggcagcgca gtgcccgaag agccagtgct
      841 gtttattaaa ccagaaacgg cactgtgcga tctgcggcag ccattggcga tcccatccga
      901 tttcggttca gttcatcatg aagtcgaact ggcggtgttg attggcgcga cgctgcgtca
      961 ggcaacggaa gagcatgtcc gcaaagccat tgccggttac ggcgtggcgc ttgatctgac
     1021 attgcgtgat gttcagggaa aaatgaagaa agccgggcag ccgtgggaaa aggctaaagc
     1081 gtttgataac tcttgtccgc tttccgggtt tatccccgcg gcggaattta ccggcgatcc
     1141 acaaaataca acgctgggcc tgagcgtaaa cggcgaaaaa cgccagcaag gtacgactgc
     1201 ggacatgatc cataaaatcg ttccgttgat cgcttatatg agcaagtttt ttaccctcaa
     1261 ggccggtgac gttgtgttga caggcacgcc tgatggcgtc ggcccgttgc aaagcggtga
     1321 tgagctgaca gtcactttcg atgggcattc tttgacaact cgcgttttgt aatacttttt
     1381 gccgccagaa agcggcggca aaacttgcat cgctgtgcca gactggttat aaggtgcgtt
     1441 ttaacgtaat ggcggaacac ctgatgagcg atgtaccttt ctggcaaagt aaaaccctgg
     1501 acgaaatgag cgatgcggaa tgggagtcgt tgtgtgatgg ttgcggtcag tgttgcttgc
     1561 ataaactgat ggatgaagac accgacgaaa tctacttcac taacgtcgcc tgtcgccagc
     1621 tcaatattaa aacctgtcaa tgtcggaact acgaacgtcg tttcgagttt gaacccgact
     1681 gcattaaatt aacccgtgaa aatctgccaa cattcgaatg gctgccaatg acctgcgctt
     1741 atcggttgct ggcggaaggt aaagatttac ctgcgtggca tccgctactt actggttcga
     1801 aagcggcaat gcatggtgaa cgtatctctg tgcgtcatat cgcagtgaaa gaatcagaag
     1861 tgattgactg gcaggatcat atcttaaata aacctgactg ggcgcagtga taaatttaat
     1921 agatgctgat gggggctttc ttaatatacg tctggatgct cgccatttcg tagccctgtt
     1981 tttgaaagcg ctctgtcata atgagagtta cccgatacct tattatcaac aggtaactct
     2041 caatctttaa aaaacgtctg tgtttttaaa tgtgataaac cttatacctt gagtacatgg
     2101 agagaataat cgcttatcag acttcaggta cttcaaagag tgtcttttta ccgtgtcttt
     2161 tctgatactc attacaggtg ttaatcattt ttttggccgc tgcttttagc aaagaaagca
     2221 ttaaatcatc ataatcaaca tagaatctgg ttgtttcagt ttccgttttt atctccccga
     2281 tggcggctat ttcggtggtt aatttcaatt tggcggcatc gatatcttta ttcgcttgtt
     2341 taaccgtgtt agacagggtg gtaaagaaac tctgcacaga ttttaacttg ttcttcaatt
     2401 ctggaatcag tttcccttca actacgcccg cagcaataga ataggaaatg attaaaccaa
     2461 atggaccggc gacgacatcg gctgcggcac cggcatacgc ttccttcctg attttatcta
     2521 cctgtgactg gaaatagctg cttttttctg aaaaatcatt ggttaactgg ctatctaacg
     2581 ccagcagttt cccggaagcg ttgttgaaac tttgtgagct taccagcagg gatttttgcg
     2641 cttcattcag cttcgtgatg ccgtcatcca gtaccttaat gaggggcgga tgctttcttc
     2701 tcattgtact catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac
     2761 cattcataca ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt
     2821 ttaatatcgc cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac
     2881 tcttttatgg tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa
     2941 agatctaatg ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cgttttatct
     3001 gcaacgattt cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct
     3061 gttttacaac tattaataaa ttttacttca tctaattcat agttagccgg gcgggatgcg
     3121 tcaatgtctt tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc
     3181 gaatgcttct attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc
     3241 gtcgccagaa ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatgcagc
     3301 gacaaatatt gatagcctga atcagtattg atctgctggc aagaacagac cactgtatat
     3361 aaaaacagta taacttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg
     3421 aaattgtgac ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag
     3481 cagattacgt tgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga
     3541 cttacttcgt caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt
     3601 tactgattgt cgatagtgct attaccgcca gccatggtga tattgtcatc gctgctgttg
     3661 acggcgagtt tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga
     3721 acagtgcgta ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg
     3781 tgatccacgt cgttaaggcg atgcgctgat gtttgccctc tgtgatgtaa acgcgtttta
     3841 tgccagctgt gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtgctatc
     3901 gaataatgac ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gcgttaaaat
     3961 gggcgatccc tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag
     4021 cagcaattat gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact
     4081 atcgccccgc gtcgagattt acagtattga tgaagcattc tgcgatctga caggtgtgcg
     4141 taattgtcgc gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac
     4201 ccatcttact gttggtgtgg ggatcgccca gaccaaaacg ttggctaagc ttgccaatca
     4261 tgcggcaaaa aaatggcagc ggcagacggg tggggtggtg gatttatcaa atctggaacg
     4321 ccagcgtaaa ttaatgtctg ctctccccgt ggatgacgtc tgggggattg gacggcggat
     4381 cagcaaaaaa ctggacgcga tggggatcaa aaccgttctc gatttggcgg atacagatat
     4441 ccggtttatc cgtaaacatt ttaatgtcat gctcgaaaga acggtgcgtg aactgcgcgg
     4501 cgaaccctgt ttgcaactgg aagagtttgc accgacgaag caggaaatta tctgttcccg
     4561 ctcgtttggt gaacgcatca cggattatac gtcgatgcgg caggccattt gtagttacgc
     4621 tgcccgggcg gcggaaaaac ttcgcagtga gcatcaatat tgtcggttta tctccacatt
     4681 tattaagacg tcaccatttg cgctcaatga accttattac ggcaatagcg cgtcggtaaa
     4741 actgctgacg cccactcagg acagcaggga tatcattaac gctgctacgc gatctctgga
     4801 tgccatctgg caagcgggcc atcgctacca aaaagcgggc gtgatgctgg gggatttctt
     4861 cagtcaggga gtcgcgcagc tcaatttatt cgatgacaac gcaccgcgcc ccgggagtga
     4921 gcaattgatg gcggtaatgg atactctgaa tgctaaagag ggcagaggaa cactctattt
     4981 tgccgggcag gggatccaac aacaatggca gatgaagcga gctatgcttt caccacgtta
     5041 tacaacgcga agttctgatt tactgcgggt caaataaata tagcggcagg aaaaaagcgc
     5101 tcccgcagga gcgccgaatg gattagcgac cgaacagatc acgttttttc gctttaaacg
     5161 gctgggaaat caccaccagc accgcgacaa tcaggtaagc gataaaaata ccgagcagcc
     5221 actgcggcat ttccatacct aaaaaatccc actgacgctc ggcgcaatcg ccagaggcga
     5281 caaacacttg cggcacccat ttatccagcg gcagccattc cgggaaacga accataaaat
     5341 cacaggtggc aaatggcgaa ggatagagct gaagcatggt gtgctcgtaa gttaactgca
     5401 caccgcggaa cgcactatac aaccagataa ccatcgctac ataacgcagc ggagttttcg
     5461 gggcgatcgc gccaatcagc gcagcaccca gaacgccgaa taacgcgcag cgttcataaa
     5521 tacagagcac gcaaggtttc agtaacatca catgctggaa ccacagcgcc gtcagttcca
     5581 gtgccagagc agtaaacgcc atcaacagcc acgcgccccg gccttgtgaa cattggttca
     5641 aaaatcgcaa cataatcatt tccctgcaat atgcatagag tgcgcagttt aaaccaattc
     5701 attcgctgcg ccaccaggag gcagcaaaaa taggacgcaa ttggacaatt atcgggcgaa
     5761 aaggcaaacc gggcatttat atgcccggta agttgttatc aaagcgttgc tatccagccc
     5821 atttgcataa accattcggt tacaggggca agcgtaaact cgacgcagag caagccgacg
     5881 agtgtcagta cgagggtgta aggcagggcc atccacacca tgcggccata agagaggcga
     5941 atcaatggcg cgagtgcaga ggtcagcagg aacaggaacg cagcctgacc gttcggcgta
     6001 gcgacagagg gcagattggt accggtatta atggcgaccg ccagcagctc gtattgcttc
     6061 aacgtgatag cgccactttc cattgccgct ttcgcttcgt tgatataaat cgtccccacg
     6121 aagacgttat ccgaaatgga tgacagcaga ccgttgaaaa tatagaacag cgacagctga
     6181 gcatgttcag atgcctgcaa cacaaactga ataatcggcg aaaacagttg ttggtcgata
     6241 atcaccgcga ctaccgagaa aaacaccgtc aacagtgcgg tgaatggcag agattcggtg
     6301 aaggctttac cgatagcatg ttcatcggtg acaccggtca atgatgttgc cagaataatg
     6361 actgacaaac caatcaagcc aacttccgcc agatgcaacg ccagcgcagt caccagccag
     6421 acgccaataa tcgcctggac aatcagacgg attttatcct gacgggtacg ctggtggcgg
     6481 ctttgatcgt caaactgttg caacacttcg cggactttct ccggcagcgt ttcaccgtag
     6541 ccaaaccaac gcagcttctc taccagcagg caggttaaca ggccacaaat cagaaccgga
     6601 acggtcaccg gcgacatgcg caggaagaaa tcgccaaaat gccagccagc cgctttagcg
     6661 atgatcaggt tctgtggttc gcccaccatg gtcattacgc cgcctaatgc ggtgccgaca
     6721 ccggcatgca tcatcaggct acgcagaaag ccacggaact gttccagaac cacgttgtaa
     6781 tgcttgtcga tatgactatc gtcttgcagg tcggtgtctt cggtacggga agaggctacg
     6841 cgatgataaa taccataaaa accgactgca acgctgatca ccaccgccac gacggttaag
     6901 gcatcgagga acgcggagag gaacgctgcc gccacgcaaa aagagagcga cagcagcatt
     6961 ttggagcgaa tgcttaacag caaacgggta aatatgaaca gcaacagctg tttcataaaa
     7021 tagatacccg ccaccataaa catcaataac agcaagactt caagatttgc cgccacctct
     7081 tcacggacgt gttccgcgct ggtcatgccg atgaataccg cttcgatagc caacagacca
     7141 ccggggagta gcgggtagca tttcagggcc atcgccagag tgaaaataaa ttccgctacc
     7201 agcaaccagc ccgcgacgaa agggctgatg aggaaaatta acgggtttac gattaagaaa
     7261 atgatgaggg cgagtttgta ccagtcgggg gactggccca aaaaattgcg ccatagcgcg
     7321 cggccccagg agatctccat gatggtttcc cttaccttac aaataatcaa tgatgttttt
     7381 atgtttaaac gcaaagctta acggtcaggc aggagtgagg caagtcttga tagtcaaggg
     7441 gaaagagatg cggaaaatga agccttgatc cctttttctt ctttttgtct gctatcagcg
     7501 tagttagccc tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact
     7561 atggtcatta aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc
     7621 tggaataacc gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta
     7681 attggcgtaa cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg
     7741 ttgaccattc aacatggcaa gccgacgaag gtgaataatt tctgggaaac ttccggttta
     7801 aatatccttg aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat
     7861 ttgctgtcgg tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat
     7921 cccgataaag cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc
     7981 tttgccgagc tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt
     8041 tacggtctga tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc
     8101 gccaatccgg aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc
     8161 agtgaaggcg cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc
     8221 gagatttggc accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa
     8281 tcccttccgt ttaaagagca accccctcaa acgaggggtt ttttgttgtt tttacagatt
     8341 tcccattctt ggcgggcaac gttccagcaa ctcgatgctg ccgtcttcgt tttgctgttc
     8401 gagcatcaca tcaaatcccc acaggcgatg cacatgcttc aggacttctt tgcgcccccg
     8461 atccagcggt gcgcgattat gtggaatata acgcagcgtc agcgaacggt cgccgcgcaa
     8521 atccacgttc cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa
     8581 ccggttacgg atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt
     8641 atgccgatca tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag
     8701 aaactggctg ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag
     8761 ccagtcggaa ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca
     8821 aatccgttta atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta
     8881 ccacgggctg ttataggggg ggctggaaga ccacattggt gtggctgtgc aaaaactcca
     8941 gcataaaacg ttccgttact ttcccttcat catacagatg gttaaggatg gtatagtgcc
     9001 agaaggtcgc acagccttcg ttcatcacct gagtctgttt ttgcggataa aaatactggc
     9061 tcaccttacg cacaatacgc aggatttcac gctgccatga ttccagcagt ggcgcatttt
     9121 tctccataaa atagagcagg ttttcttgtg gttcggacgg atagcggcgc gcttcagcaa
     9181 ccgttttctc ttcctcgcgc ttcggcaggg tacgccagag catattgacc tgactttgta
     9241 gatactcttc gcgacttttc tgccgggctt tctcttcttg cagcgagatt ttttgcgggc
     9301 gtttgtaccg gtccacgccg tagttcatca gcgcgtggca cgagtccaga agccgttcta
     9361 cttcatcaac gccataacgt tcttcgcact cggtaatata tttacgggca aaaatcagat
     9421 aatcgacaat cgaactggcg tcggtccaac tgcggaataa gcaattgttt ttaaagaaag
     9481 agttatgtcc atagcaggcg tgtgccatca ccagcgcctg catagtaatg gtgttctctt
     9541 ccatcaagta agcgatacac gggttagagt taatgacgat ttcataggcc agcccttgct
     9601 gaccgtgctt atacagccgt tcagtctcga taaacttttt accgaatgac cagtgcggat
     9661 agttaattgg catgccgacg ctggaatagg catccatcat ctgttctgag gttatcactt
     9721 caatctggtg cgggtaggta tccagccggt agagtttcgc cacccggtct atctctgcca
     9781 gataaacatc cagcaggtcg aacgtccagt cgggtccatc gctcaaacgt gtggtgtcct
     9841 tattcataga atcgatcgtc gccatacgcg cacctcattg ttgtcggcgc tctctgtgtg
     9901 gagcacctca tttcaagcat agaacacctg ttaaaaaccg cgtcgccgga gaattttttt
     9961 ctttgcgatt tcttattatc agagtgccac taatccgctt ctgaacggaa ttttatgctg
    10021 gataaaaagg gcgttcagca ggagatacta aagacgccat attgccgcag agtcagggag
    10081 atgtgagtca gctcaccata aaaaagccgt atgttgaata atattttcaa ctgagttatc
    10141 aagatgtaat tagattatta ttcttttact gtatctaccg ttatcggagt ggctatgcga
    10201 gttgtcatac tgggaagtgg tgtggtaggc gttgccagcg cctggtactt aaatcaggca
    10261 ggacatgagg tcaccgtcat tgatcgggag ccgggggccg ccctggaaac cagtgccgcg
    10321 aatgccgggc aaatctcccc cggatatgct gcaccgtggg cagcaccagg tgtgccttta
    10381 aaagcgatta aatggatgtt ccagcgccat gcgccgctgg cggttcgtct cgacggtacg
    10441 cagtttcagt tgaaatggat gtggcaaatg ttacgtaact gcgacaccag ccactacatg
    10501 gaaaacaaag ggcggatggt gcgtctggcg gaatacagcc gtgattgcct gaaagcattg
    10561 cgcgccgaaa ccaatattca gtatgaaggg cgtcagggtg ggacgctgca actgttccgt
    10621 accgaacaac agtatgaaaa tgcgacccgc gatatcgccg tgctggaaga tgccggcgta
    10681 ccgtatcagc tgctggaatc cagccgcctg gcggaagtgg atcccgcgct ggcagaagta
    10741 gcgcacaaac tgacgggcgg cctgcagtta cccaatgatg aaaccggaga ctgccagctg
    10801 tttacccaga atctggcgcg gatggcagag caggcggggg ttaaattccg ctttaatacg
    10861 cccgttgacc aactgctttg cgacggcgag caaatctacg gcgtgaagtt tggcgatgaa
    10921 gtgattaagg ccgatgcgta tgtgatggcg tttggttctt actcgacggc gatgctcaaa
    10981 ggcattgttg atattccggt gtatccgctg aaaggctact cgctgactat tccaattgcg
    11041 caggaagatg gtgcgccagt atccaccatt cttgatgaaa cctacaaaat cgccattacc
    11101 cgtttcgata accgcattcg tgttggcgga atggcggaaa ttgttggttt taacaccgag
    11161 ctgttgcaac cgcgtcgtga aacgctggaa atggtggttc gcgatctcta tccacgcggc
    11221 ggtcatgtcg agcaggcgac tttctggacg ggtttgcgcc cgatgacgcc agacggtacg
    11281 ccggttgtcg ggagtacacg ctttaaaaat ctgtggctga ataccggtca cggcacgctc
    11341 ggctggacga tggcttgcgg ttccggtcag ttgttaagcg atctgctctc tggtcgcacg
    11401 ccagcgatcc catatgagga tctaagcgta gcgcgctaca gccgtggatt tacgccatca
    11461 cgtccgggcc atttacatgg cgcacacagc taaggaaacg agatgacccg tccgatacag
    11521 gccagcctcg atctgcaggc attaaaacag aatctgtcca ttgtccgcca ggccgcgccg
    11581 cacgcgcgcg tctggtcggt ggtaaaagcg aacgcttacg ggcatggtat tgagcgtatc
    11641 tggagcgcgc tcggggccac cgatggcttt gcattactta acctggaaga ggcaataacg
    11701 ttacgtgagc gcggctggaa ggggccgatc ctgatgctgg aaggattttt ccatgctcag
    11761 gatctggaga tttatgacca gcaccgcctg accacctgcg tacacagcaa ctggcagctc
    11821 aaagcactgc aaaatgcgcg gctaaaagca ccgttggata tttatcttaa agtgaacagt
    11881 gggatgaatc ggttgggctt ccagtccgat cgcgtgctta ccgtctggca gcagttgcgg
    11941 gcaatggcga atgttggcga aatgaccctg atgtcgcatt ttgctgaagc ggaacatcct
    12001 gatggaattt ccggcgcgat ggcgcgtatt gagcaggcgg cggaggggct ggagtgtcgg
    12061 cgttcgttgt ccaattcggc ggcgactctg tggcacccgg aagcgcattt tgactgggtt
    12121 cggcctggca ttattttgta tggcgcttcg ccgtccggtc agtggcgtga tatcgccaat
    12181 accggattac gtccggtgat gacgctaagc agtgagatta ttggtgtcca gacgctaaaa
    12241 gcgggcgagc gtgtgggcta cggcggtcgc tatactgcgc gcgatgaaca gcgaatcggc
    12301 attgtcgccg caggatacgc cgacggttat ccgcgccatg cgcctaccgg tgctcctgtt
    12361 ttagtggacg gcgtgcgcac catgacggtg gggaccgtct cgatggatat gctggcggtt
    12421 gatttaacgc cttgcccgca ggcggggatt ggtacgccgg ttgagctgtg gggcaaggag
    12481 atcaaaattg atgatgtcgc cgccgctgcc ggaacggtgg gctatgagtt gatgtgcgca
    12541 ctggcgttac gcgtcccggt tgtgacagtg taacttgttg taagccggat cggaggcagc
    12601 gtcttctggt tgcgaaaaaa tcatccatcc ggctggtcag caactgtagt tgttaatgtg
    12661 acagagccat tgcccatgat agtgtccatt aaaagaatgg acactatttc cccggaacct
    12721 gaactcgccg cacaggcgtt ctacataaaa cgcttacgct tcattgttga ctcggagtcg
    12781 acttcagata aatcacgctt cacccttgat gcagattcgg gttcatgttc agattgttga
    12841 ttaacaggct gcgagtcctg ttgtctttcc agactcaaca ggcccaactg ttgatccagg
    12901 agctccaggt cctcctgtac cttcacgcgc tccaggagtt ccgggtcatc caggaactcc
    12961 agatccataa ggtcctccag ctcctcgagt ccttccaggt caacctagcc ttccttgtgc
    13021 aaaaagtcca aaaggtcctt caggccctgc tgttttttca ggccctcgac ggctttgagc
    13081 agcgtcggta aattgtcttt gtcccacggg ttaccattac agtcaaacac cttcaggctc
    13141 tggggctgcg gcacctgtac cgttttaaac ttgttttctc cacagtagta aattattagt
    13201 ttgggcggca gccgtggtga agagatcaaa tcgttactag aacagtcaaa tactttcagg
    13261 cgagtcaagt tctccagcat ctcatcaggt attgagttaa tattgttgcc ggcacaggat
    13321 agtttataca ggtactgggg catcgacggc ggtaaaaatt gcaagaagtt ttcagaacag
    13381 ttaatcactc tcaggttagt cgacagctca ggcagccaga taatactgtt ttcatagcac
    13441 ttcagtacca tcagttttga gggctgcacc ttcggtaaca agatcaagac gttatctgta
    13501 cagtcaagca ccagcagcca ctttggcatc accttcggta acgcggtcaa cacgttttta
    13561 ctgcagtaaa attcaatcag atgtggaggc aaattctcag ggagtgaagt caatcccagg
    13621 ttgctaagat tgagcatact ccgtcttgta atgagacatt ccttcattct gcgcaatgct
    13681 tctgcccggt tttcaatccg gcttgctggc aggttttctt ccaggatatt ttccttgatt
    13741 tcttccagac tttctttcag gtcttcttgg ggaatttctg actgtttgtt ttgctggttt
    13801 tcttccagga tttcgtacag gtttttttgc aggttttctt gcaaaccatc ctgctgtata
    13861 gcccattcgc accatttggc gaccatcatg aactcgttat gttgctgatt cagacgagtc
    13921 cccaacgtga tgatggtatt gttgtccacc tgtatgttca ctttgcccgc gatactgagg
    13981 tcaatgatct gccagggggc atcaccccag ctgaattttt gcgggctgag atacttcatg
    14041 ttctgcaaaa tttgattctt gagaagagcc gccctggcgg gcgtcgtacg cctgggttaa
    14101 cggaagatgg gggaatggcg ggggcgaatg ggcagatgtc tttaaagcta gccataatgc
    14161 tacgtttcct tgaaataaat atcctctggc agcgccggag acgtgaatgt ggtcaccgca
    14221 aaggagccac aggacgtcat ttgcgctgat atcactactc atcgttgact tttttcctgg
    14281 tcagaaatgc cgtgtcgggt ctggtacatg ctgtattacg gcctaggtag ctctgacgta
    14341 ttaaagaaaa tacgagagtg aacgacccgg aaataatcgt tattaagcaa aggaataact
    14401 ataagaaatc gctcatgcat ccggtgctac aggcggatga aacgcctatt gtgtgggcta
    14461 tgtcagtgcg gcaggttgcg tgggggaata tgttcaggcg atgtcaggca ggggcccctg
    14521 atggttgacc ggtaatgcgg ggctggtttt tattacctgt tacagtcgga aaaagccctt
    14581 acgctgactt tcttgagccg gtgttttgag ttcattagcc agagcctgca atggtttgtc
    14641 atccataatt taaccttctt ctgatgttgg gatgagcata tcaaaatcag gaaatgacaa
    14701 gtttattaca ggctcaggct gcgttcagta aagcatcgca tccggcgata gatgctgcat
    14761 taaatttaag attcagcttc ctcttcagcc acccgaacgc caatcttcaa cacttcatta
    14821 tcttctttct cggccaccgt ccagatcatc ccggcaaact ctacctggtc accgacaacc
    14881 ggtgccgcgc ctagcaactg ctggacaatt tcacccagcg tttgctgttt atcacgatat
    14941 tctcgcccgt cttccaggcc atatatcagc gcaacatcag catatttggc gctggcttcg
    15001 agaatgaagt caccaaagaa gcgttgatcc agcgcgaccg gtggcgactg gctgaacagt
    15061 ttaccgagcg ccgggagatc gcgttcccga ccaattacac acaatacatc gccttcgcgc
    15121 agtcgggtgc tgccggtagg atgaagcaac tggttatcac gaaacagggc tgcaatacgc
    15181 gtctcttttg gcatatgcaa atcgcgcagt gccgcaccta cacaccattt atcagcactc
    15241 aattgataaa caaactgctc ccacggattt tccggatgaa tatccaggcc aacgcgtgac
    15301 accggacgtc cgacgggcgg aaccaccact ttggcttttt tcgccgccca cgagagtgat
    15361 gttccctgca agagcagtga aaccagaacc acaaagaagg cgacattaaa gaacagtcgc
    15421 gcattctcca gccccgccat catcgggaac acggctagga tgattggcac cgcgccgcgt
    15481 aatcccaccc agctgataaa cacgcgctca cgcagattga aaccgcggaa agggagcaat
    15541 ccagcaaata ccgaaagggg acgggcgaag aatatcatcc atgccgacaa aatgagcgcc
    15601 ggaatggcaa taggcagcag atcgcttggg ttaaccaata gccccagcac caggaacatg
    15661 gcgatttgcg ccagccaggc gaggccgtcg aaattttgca ggatgccgta gcggttgcga
    15721 atcggccgat tacccagcag aaaaccgcac agataaacag ccagaatacc gctgccttcc
    15781 agcgccgtgg ttaaagcaaa aattaaaata ccgccgctta atgccagcaa tggatataat
    15841 ccggcaggca gggcgatgcg attaatcatt tgcagcagta aataaccacc gccaagaccg
    15901 atgacaatcc cgaggccaaa ttgctgcaga atatcgacca cgaacatcca actgacactg
    15961 ctttcatgct gctggatcat tgcaatcagg gtaatcgtca gaaagaccgc cattggatca
    16021 ttactgccgg attcaatttc cagcgtcgag ccaacacgtt cgttaagccc cttaccaccc
    16081 agcagagaaa agaccgctgc agcatcggtg gagccgacga tagcgccgat taataagcct
    16141 tcaatcaaat caagattaaa cagccacgcc gccatcatgc cggttaaacc agaggtgata
    16201 agtacgccca gcgtcgccag cgacagcgcc ggtcctaacg ccacacgaaa ggaactggcc
    16261 tgagtgcgca tcccgccgtc gagcaaaata atcgccagcg ccaggttact taccatgtag
    16321 gcgaaggggt aattatcaaa cgggataccg ccgacgccat cgactcccgc cagcatgccg
    16381 atcgccagaa aaataaccag aataggaatg ccaagacggg aagaaaatga actaagtaat
    16441 atactgctgg tgacaaggat ggatcctaag atgaaaaggc taattattgt tgtggcatcc
    16501 aatggtcggt tactcctgat tacgctgtct cttatataaa ccctaccata ttagcggcag
    16561 agacagcgtt ttacttagcc ctgaagcgtg atttttttac attttaagaa caggatgacc
    16621 gctaatcgtc aactgagcgc cttcccgggt gttattcaga atggcatgtg cgcccaacgg
    16681 cagcgtaacc gtgcgttgtt catgaccaaa atcgaggccg gtaatgagtg gaatcgacag
    16741 gcgggaacgc aaaaacgcgt acactgactc caggttgtaa cctgcgtcat aatcattggg
    16801 cgtgctgccg ctaaagctac cgagaataat cgccttctga cgtggcaaaa ttcccgcatg
    16861 atagagctgc aacagcatac gttcgacgcg gaaagggtgc acgttaatat cttccagcac
    16921 cagaataccg ttctcaattt ttggcatcca cggtgtacca atcagtgaaa tcagcatcgc
    16981 aagattgcct ccccacaacg tgccttcggt ctgacatgtc gggccttccc cttgccattc
    17041 aatggtgaag gtttcattgc gtaacgccag ccagaaatgg tgctcggtaa aggcgttcag
    17101 ttcatccgca ccaaagtttg ctaccagcat ggggccgcta aaggtgatga cattgccttg
    17161 cgccagaaga ccgcactgaa tggcggtaaa atcgctatgt ccgcaaatga gtaacgggtc
    17221 atgttgttgg cgagccacca gtgcctgcca gtcaatatct gccagtaaac gactggcacc
    17281 gtaaccgccg cgtacagcca gcacgatggt gttgggggta gtcagtctgg caagggaatt
    17341 gagatcttcc agacgctccg tttccgtacc cgcaaaacgc tcacaacgac gggcaataac
    17401 ctcgacgtta ttgacttgat gccccgcgtc ggtaaggcgc tggataccac gcagcgcggc
    17461 gtgctgttta atgctgtaac ccgatggggc aattaagtga aacagagaca tggcaattcc
    17521 ttgctgacaa cagaaacgaa atgtatatca tgccgcttag gtgtgccgtt gtcacctcaa
    17581 cggcgattcc aggctataag gatagaagaa gtgaaattga gatggtttgc ctttttgatt
    17641 gtgttattag cgggttgttc atcaaagcat gactatacga acccgccgtg gaacgcgaaa
    17701 gttccggtgc aacgtgcgat gcagtggatg ccaataagcc agaaagccgg tgcagcctgg
    17761 ggcgtcgatc cacaattgat cacggcgatt atcgctatcg aatcgggtgg taatccgaac
    17821 gcggtgagta aatcgaatgc cattggtttg atgcagttaa aagcttcaac ctccggacgt
    17881 gatgtttatc gccgtatggg ctggagtggt gagccgacga ccagcgagct gaaaaatcca
    17941 gagcgtaata tttcaatggg ggcggcttac ctgaatattc tggaaaccgg cccgctggca
    18001 ggcattgaag atccgaaggt actgcaatat gcgctggtgg tgtcatacgc taacggggca
    18061 ggtgcgctgc tacggacttt ctcgtcagat cggaaaaagg cgatcagcaa aatcaacgat
    18121 ttggatgctg acgagttcct cgatcacgta gcgcgaaatc atcctgcgcc gcaggctccg
    18181 cgctatatct acaaacttga gcaggcactg gacgcgatgt aaatcagtcg cgcactttgt
    18241 ccgctttttc ccgggcttct cgctcgagag agaaaataat ccgctgtaat tgccgctcca
    18301 ccgtcgggct gacgttaaga aaacggaagc tcagacgggg agtggtgatg gtttcattct
    18361 tgccatcaat cactttgcgc tcgctggtgg agattaactg ggcgtcaaag tgaaaaacac
    18421 cccattgccc catgttgact tcaatttgag cgaagcgcat accttcttgt aattcggcag
    18481 gctttgctgt ttccagtaat gcgcccatgc cgcctaacga caaatcatac aagcggaaac
    18541 gtaacgtact gttatccgcc agtttggtct ggcaaaaata aggcggatgg agtggggcgg
    18601 agatgcggaa atatcggcgt cgttgtacaa accataaggt gggaggcggt acggtaataa
    18661 atgccggaag ctgcaagtat tcactctgct gtagttgttc aacagtaaac tcgactttcg
    18721 caccctgagt ttcggcggta atggtaatgt gctgtgcctt tagcacggcg atgttgtctt
    18781 cggcttgact gccgaaatcc agcaccagtt tatccggggt tattgccagt attttgctga
    18841 tcagctgccc gccattccaa ctgagacgca aaggaattgc ggctttgtgc aaatcgcgta
    18901 acacgcccag gacggctaac ggattttgtt tcaggaactg ctcatggtaa tgactcacgc
    18961 ggaaaaactc ctgactgaca aactgtcttt ggtttatcgg tcatagttaa caaaacttaa
    19021 tacaaatgcg tgaatatttt ttacatgttg ttcttaaatc agccgacaaa cgcccagcat
    19081 cgatcccgcc cttgcctata cttagagcgt tgacgtaagc atttcttgcg accgattcat
    19141 cgaaacgagg gcctgaacat gggaattatt gcctggatta tttttggcct gatagccggc
    19201 attatcggca agctaatcat gccggggcgt gatggtggtg gatttttcct gacctgtatt
    19261 ctcgggatag tcggtgcggt ggtcggcggc tggctggcga ccatgtttgg cattgacggc
    19321 tccatcagcg gttttaattt gcacagcttc ctggtggcgg tggtgggcgc tatcctggtg
    19381 ctgggcgtat tccgcctcct gcgaagagaa taagattttc ataaggcgga tagcgataca
    19441 gatgccgcta tccgctttca catcagaacg tatactcgac acctgcccag aaattgcgac
    19501 cttcttcaat aaacccttca ctgtaggcat attgggtatc aaacaggtta ttgaccgacg
    19561 cattgacgct gaagccatga cctaaggtgt aatcggctcg aatgtgggtc accgcaaaac
    19621 cggcggcttt ttgtgaaccg tcactgttgc tgtagctgga ggaacgcgcc tcttccgaca
    19681 gcgttacgct taacggctcc catggtttga gtgtcatcca tgcggtcatt gtctgcgttg
    19741 gaagatcggt tatcttgccg atgtctttgc gtttggcgtc agcgtggatc agagcgtagc
    19801 tcaatcctac atccagtata ttgctgattt tccccttaat accggcatcc agaccgctgt
    19861 aatccaccgt gccgctgttc tgattttgaa tggtatcggc attgatattg tgcgagagga
    19921 tggcatcact cacccggtta tagtaaacgc tgacctcaaa tccccagtcg cgcgtgaagg
    19981 caccattcca ggttaaatcc accccgcgag cgcgttccgg cttgagctgc gggttaacaa
    20041 tcgctatctg gttatacgca ggtttggatg tggtatagcg ttctttcagc gtcggaaagc
    20101 gtgtgcggtc atagtacgaa agcgccagag tgtcttcatt ggcaaagtgg tatttcccca
    20161 tcacctgcca gttaaaagct gactgattgt tgtcgtcata atgggtgatg ctgccatctt
    20221 tctcgtgttt tttcgcttct acgctatcgc gccagtcata gctgattccc gccacgacat
    20281 cgacattatc agcagcagcc cattgatatt cactggcgag cgaccaggta cgatcttcat
    20341 agcgatcgta agcggcgtgc ggcgcacctt tttcccggtg tacgtcatct ttccagttaa
    20401 cggcaaacga cagcagatcg ttttcacgca catcggctgc cagttgtaat ccggcaccgt
    20461 cgctgtaatc ggaataatgg ctgtagctgc cttttttatt tttcaaatca gccagcgagt
    20521 tgtacatcat cagcgtattt tcaaaggtgt cgcgatacag ccgacttttc agggtaaaac
    20581 ggtcgtttag ttgggtcgct ccctgataat aaaaactttc tttgtcatac tctggccact
    20641 gccagtagcg tgatttttga ccacaatttc cgctgtatgg cgggttatct ttttcaccat
    20701 cctgcttaat gtaagtcagc gtgtattcat cgttttcgcg cggtgtaaaa ccgagcttca
    20761 caatgccgcg tttatcatca gccgatgaat taatcatctt gccgtgtttg cctgcaatat
    20821 cattatttac accatgcggc aggccgagaa aatcctgctt tagctggcta ccgctgactt
    20881 gcaaataccc aagatcgctg ctggcggcaa atgaagcatg catatcgtag gcattgtcct
    20941 gactacggct ccatccctgg cgatatccca gacttgcttc ttcagggtgg tgatattaat
    21001 ggctccgccc atctgattag gcccatgaag cagcgacgaa taccctttgg aaacttcgac
    21061 tgcccccaga ttgttggtca gaatccgcgc cagatcgagg ttgccgtcat aggggacata
    21121 aatgggcaca ccgtcgaaat agaccggcac ctgccgacta tcaaagccac gaacttttac
    21181 ctgttcttcg ttgcggctgc ctgacttttg cagcaccacg ccggggacga cacttaatgc
    21241 ctgagcgaca ttctgtttat ccaaggcctt catggtgggt tgatcgagaa cggtggtcgt
    21301 cgtcgatgat accggactgg accatacggt cagcgtgtcg ctctcctgag ccgctacggc
    21361 ctgctgggta aaggcaagtg ataatgctgt gcagagataa cgttttttga gtctcataga
    21421 ataaattccg tttaacaaag aattaaatag aggctgtggg ttgcagactg actttaatgt
    21481 cagctggtgc cgcgtaataa ggcgcggagg taataaaaag gcgaatgccg cagtcgaggt
    21541 aatttttgag tgtcgtcaga ttaattccgc cggtcagagc cagcgtgcag tgaggagcca
    21601 gcgacggcgc tatttgcgca atttcagttg cctgttgcgg actaaatttg tcgagctgaa
    21661 gtacgtctgg ttgtgcgcgt aacgcggcga tagcctcttt cggcgtgtcg gcttcaacaa
    21721 caattttctt ctcgggtgcg tggcgacgta actgagcgat tgcgcctgac cagccctgat
    21781 tgtcatgaag aaaatggcgg tggttggcaa acaataatat ggtttccgca catccggcac
    21841 gatgaattag tcctccggca gccagaattg cctgcgaagc cagtaagcgg gtgcccggaa
    21901 ttgcttttcg ggtgcaggcg atattgccgt caggataacg ttcacgaagc aatgccagca
    21961 tttgagcgag ataatcagaa acaccgcaac tccactccag cacattctgg actgccttcc
    22021 atccctgatg aagtgctgcg gcattaccct gcgcacggat tagccgttga cccgcgttcg
    22081 cttgtgaacc gtcgctgacc gcgtcatcaa tagttaatcc cagcgtagtt aacatcttac
    22141 acgcgacaga aacgccgctg acgcagccgc cctgacggtg aaaaaacgct atatagccag
    22201 gctggtgtcc aatatttaat gctcgcgtgg tcaggtcacc tccctggata tcttccagca
    22261 gtaacgcatc gatttgtgcc tgagaaagga aaatcatcac agtgcctcct gcggtacgct
    22321 atcccaccag atgagcgccc agtcgcggct ggcaggggcg agggcacgag catcttgttg
    22381 ttgataccag tgatataacc gctcacgctc atcgtcgtta agcgcgccaa gactccagct
    22441 gacgttctgc tcaaaacgtt cccaggtgct gttatcctgt tggcaatttt gtccacggat
    22501 aaaatcaacg tgggcataaa ttcccatctg atacaggacg ttgagcgcaa agatatagtt
    22561 gggtaattcg atcacttccc gaccagccgc ccgctgaatg gctggagaga cgaacgaggt
    22621 gctgaccaga tgagttgtat agacgcgaag ccgtgcctga ttattcagtt tgctcattgc
    22681 atggcgcata tcagccacca gagtggagcg ggaagccacg gcaatatcgc agcgtggcag
    22741 gtcactccag tcctcttccc aggcgcgctg gatccagtgg acattgtcag ccttcagtgc
    22801 ggcggcgcgg cgggcggcaa cgttcagcat cccctgactg taatcaacgc cataaaccgt
    22861 agtgagtttg tcagccagcg ccagcgatac ggtgcctggt ccgcagccca tatcgaataa
    22921 ggtctgagcg ccttgcaggt caattttagc gatcagttgt tgcaggtagc tgtccgttgg
    22981 cgaggcgcaa ttttccgcca ttttctccgc ccgttgatcc cagtgatcgg gcgttttttc
    23041 tgtgcggtga gccagtttga gttgctgcag ataaaggtct gcaaaatcaa tatcatcaat
    23101 gagcatgatt gttccttagc ggctaattgc agagaggtga tgatgaattt ggtcggcaga
    23161 aacccgatac agggccgcca gtttgttcgt tgttaactgt tcggtcggta agccttgtgt
    23221 tactcgtcca tctggctcta cttggataat gctgtcggca atggcgttgg catggagcgt
    23281 atggtgagtc gacattaaca ttgtcatgcc gttatttttg agctgcgcca gcgtgtccag
    23341 aagttggatc tgatgaccga aatcgaggct ggaggcgggc tcatccagta acaacaagcg
    23401 cggttgttgg acgagtgcac gggcgattaa agccagctga cgttcaccgc cgctaagcgt
    23461 attccagcgt cgtgtcgcca gatgcaaaat gcccagcttt tccagttgct ccgtggcttt
    23521 caggcgttct tgcttaccgg gaacggaaaa agcgcctatg gtgggggcca ggcccatcaa
    23581 caccatatcc agcacactga aagcaaaaat accgtcgtgc gcctgcggca cccaggcaat
    23641 agcctgtgcg cgctggcgat ggctgagtgt ctgcacagag aggctatcca gtaggatctc
    23701 gccttttaat aacggtataa cgccaagaat ggagcgcatg agtgtggttt tgccgcaacc
    23761 attcgcgcca agcagacaac aaattgttcc ttgcgacagg gtgaaggaca cgtcgcgtag
    23821 cactggatgg cgggagtaac ccagagacgc atgacgaacg gcaagcaaag tcactggcga
    23881 cctccgcgta acaacaaaag caggaaaaac ggtgcgccaa caaaagcggt cagaatgcca
    23941 agtgggattt ctgttgttcc aatgctgcgc gccagcgtat cggtaagcag gagcagaata
    24001 gcgccagtac acattgccat aggcaacagc tgctgatggt tatgaccggt caacaagcgg
    24061 gcaatgtgcg gaacgaccag accgatccaa ccaattatgc ccgctattgc taccgtgctg
    24121 gcggtgatta gtgtggcgca gacaatcaat ccaaagcgca gacgcgtcac gttcagtccc
    24181 agcgaacggg cttcgtcgtc ggaaagcgtg agtaaattca tgcgccagcg gagaaaaaac
    24241 aggggcaagg agcctatgag aatgatcggt gcagcatagc agagatcgcg taaggtcacg
    24301 gtggacagtc cccccagcaa ccagaaggta attgacggta actgggtgta aggatcggcc
    24361 agcgttttaa ttagcgatat ccccgctccg cacaacgttc ctaacgcaat gcctaccagc
    24421 accagggtta agatcggatc gtggcgggtc acccgacgcg taatcaacca gacgcccgcg
    24481 accaccatca gcccgccgca aaatgcgtac agctggatgt aaacaatggg taatccccac
    24541 aggattgccg cgcaggcacc aagtccggca ccggcggaga cgcctaaaat atccggtgaa
    24601 actaagggat tacgcaacat tccctgatag gtggttccgg ctcctgccag cgctgcgccc
    24661 agtaacagcg cagcaagaat acgcggcacg cggatttgcc agaagacaat ttgttcctgc
    24721 acagactcat tttcttgacg aagaattagc gccagcagtt gctgcatatc gagatgataa
    24781 gccccactta cggcggcaaa agtgatactg atagctgcca gcagcaaaat acatccgttt
    24841 acgattctca ttagctcgcc accaattttt gatagtcggc atcactgagt gaacaatgcc
    24901 agaacagttg ggcgtaatgc tgcatgtcac ttttaaactg gcgattgatg gcgggatcca
    24961 gccaggcgtg aagtctgcgt aatcccagaa gacggttgat tcccggcggg gcatcgagcc
    25021 agccaaaggg caagccactt aaaaataaga tgcgttgttc cgcaacggct ttcacgccct
    25081 gccagagagg atcacgacga ataaaatctg cagtaacggc ctcctgaacc agaataatat
    25141 ccggctgcca gcgcaggaga ttttccatgg aaacctgtgt caggccgtgg cgatcggcta
    25201 tttgcgcgac gttgtgcaaa cctaataatt ccgccgcctc ggtatgcaac gaaccctgta
    25261 agcccgtttc cagccctcgg ggaccgcgcg cagcataaaa gcggaggtta gcggcgggtg
    25321 aggtggcgaa tgcctgagct tcaccaacga agtggctggc gagattggct tgttctgcgg
    25381 cgcggtgctc ttcgcctaac gttttgccaa gcgttgttaa ctgttctgct gattgcgcca
    25441 gttttccgtt aagcaataac caggggattt gtgtctgttc gctaacctgc cgtgcctggg
    25501 agatcaaggt ttcatccgta ttgccgcaat caacgaccaa atcgggatgt aacgccataa
    25561 gcccttccag cgatagtgtg ctggcgcgcc cggcgagtcg tcccagcctg ggtaactggc
    25621 gaatgtgctc tggcaatgga attaatgcct gacgggcaaa atcaaaagag gaaaaaccga
    25681 ccattttttc tggcgcgaca gccagcaaga gcaaatcggc gggcgcgcct gcgctgacga
    25741 tgcgtgtaat gttttgggct tcgggaagag attgcgaagc aaaacgatta actgtttgtg
    25801 aaaaggacgg tagagactgc aggagcaggg tagatgcaag tgcctgcgca aacgtccgtc
    25861 tggttatggg catggtgaac atcctgaaaa gtaaaatcga tatatacttt catatatatc
    25921 gataaaaatc atgttcaata tcaaagttta gctattgaaa acatgaagat gtgacgactt
    25981 ccgcaagcct gttgaccctc tctatcatta aaaatgtatt tatcaggtgg ctaatattat
    26041 ttaaatcatt taaggctaaa ttatttaaat atgaatgaca ttattgtttt tttatcgtcc
    26101 acggttacgc gacgttgaag ttacttgttt tataatgagc gatttttgat atctcgccga
    26161 gaaaattatc agcattctct cgtttcagtg tcgtgaatat actgttaacg gaggtgaaaa
    26221 gaatgctggg ggttatacaa aacctatgtt gttgtcgtcc ggattcaccg cttgctgata
    26281 gtaagcagcg taaccagaag ttaccgcaac aagactttga ggattttaag aacgacgtta
    26341 ttaatgtcat taacaacaat gaagatattt attttcttag agagagtaat ttactgaatg
    26401 cgtttattcg gacgtgcaaa aaaagagatg ataacagtta cggaaatatg atatttacgc
    26461 tttattttga taacgatatc tatgggaaca gaattatcaa agcatcgctg aacggcggag
    26521 agttgcagtt attaagcaca gggtgtagca atgataacat tctcc
//
LOCUS       DAETQL010000056        26029 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.056, whole genome shotgun sequence.
ACCESSION   DAETQL010000056 DAETQL010000000
VERSION     DAETQL010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.056"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(409..990)
                     /locus_tag="K0W65_002534"
     CDS             complement(409..990)
                     /locus_tag="K0W65_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095757.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(1037..4894)
                     /gene="sigA"
                     /locus_tag="K0W65_002535"
     CDS             complement(1037..4894)
                     /gene="sigA"
                     /locus_tag="K0W65_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HBI3095758.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(5255..6259)
                     /locus_tag="K0W65_002536"
                     /pseudo
     CDS             complement(5255..6259)
                     /locus_tag="K0W65_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            6244..6621
                     /locus_tag="K0W65_002537"
                     /pseudo
     CDS             6244..6621
                     /locus_tag="K0W65_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6660..7007)
                     /locus_tag="K0W65_002538"
                     /pseudo
     CDS             complement(6660..7007)
                     /locus_tag="K0W65_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(7053..7223)
                     /locus_tag="K0W65_002539"
                     /pseudo
     CDS             complement(7053..7223)
                     /locus_tag="K0W65_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(7365..10880)
                     /locus_tag="K0W65_002540"
     CDS             complement(7365..10880)
                     /locus_tag="K0W65_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI3095759.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(11333..12595)
                     /locus_tag="K0W65_002541"
     CDS             complement(11333..12595)
                     /locus_tag="K0W65_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBI3095760.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(12793..12868)
                     /locus_tag="K0W65_002542"
     tRNA            complement(12793..12868)
                     /locus_tag="K0W65_002542"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12833..12835),aa:Phe,seq:gaa)
     gene            complement(12974..13681)
                     /locus_tag="K0W65_002543"
     CDS             complement(12974..13681)
                     /locus_tag="K0W65_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBI3095761.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            14079..16214
                     /gene="speC"
                     /locus_tag="K0W65_002544"
     CDS             14079..16214
                     /gene="speC"
                     /locus_tag="K0W65_002544"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBI3095762.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(16263..17519)
                     /gene="nupG"
                     /locus_tag="K0W65_002545"
     CDS             complement(16263..17519)
                     /gene="nupG"
                     /locus_tag="K0W65_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HBI3095763.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(17721..18800)
                     /gene="mltC"
                     /locus_tag="K0W65_002546"
     CDS             complement(17721..18800)
                     /gene="mltC"
                     /locus_tag="K0W65_002546"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBI3095764.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(18865..19140)
                     /locus_tag="K0W65_002547"
     CDS             complement(18865..19140)
                     /locus_tag="K0W65_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBI3095765.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(19168..20220)
                     /gene="mutY"
                     /locus_tag="K0W65_002548"
     CDS             complement(19168..20220)
                     /gene="mutY"
                     /locus_tag="K0W65_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBI3095766.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            20381..21100
                     /gene="trmB"
                     /locus_tag="K0W65_002549"
     CDS             20381..21100
                     /gene="trmB"
                     /locus_tag="K0W65_002549"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBI3095767.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            21100..21426
                     /locus_tag="K0W65_002550"
     CDS             21100..21426
                     /locus_tag="K0W65_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HBI3095768.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            21610..22329
                     /locus_tag="K0W65_002551"
     CDS             21610..22329
                     /locus_tag="K0W65_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBI3095769.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            22505..23551
                     /gene="ansB"
                     /locus_tag="K0W65_002552"
     CDS             22505..23551
                     /gene="ansB"
                     /locus_tag="K0W65_002552"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBI3095770.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            23668..24630
                     /locus_tag="K0W65_002553"
     CDS             23668..24630
                     /locus_tag="K0W65_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBI3095771.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(24731..>26029)
                     /locus_tag="K0W65_002554"
     CDS             complement(24731..>26029)
                     /locus_tag="K0W65_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBI3095772.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         6696 a         6216 c         6274 g         6843 t
ORIGIN      
        1 gaataacaca tccccgtcaa tacggccctc gctgtacgct tactcatcac ctgctattaa
       61 tccccgagtc agtacttcgc ggcctgacgc gatcacccta ctcagtactt cagagcccgc
      121 ctccggtacc tgcctggcga tcgcccctgt gcaacaaaat aaatcaaccg ttatcaaacc
      181 acgatcgttc gtatgcgccg ccgatgacgc gcgcctgaaa aacgcgtcaa tatgagttcc
      241 ccggaagctt acgataatac gcttatgaac attacaacac gctcatgcgg aacctggatg
      301 ggggaaggaa cggtgaagct gttagcgaat gaccggcgtt tatacaaacg ccggtcatga
      361 tgaatgatat ttcagaatat acctcgggct aaaataccgg gacagagatc aggcgtgatt
      421 aatctgcagc atttttaata acagaagtac tgagcttact ttacctttga tgatgtaata
      481 gttatttccg tcggtactca tttttccctg aaataatgtc acggatgttt cttcctgctg
      541 atcctgcagg cgcagtcgta aggtcatatt tttaatgagc tgagatccgg gtggcaaggg
      601 gtatttgctg tcagatactg ggcgactgag tctgatgtgg cgcgtataca gcccgttttc
      661 gtacagggtg gcctctgtta tggtgtcagt gaccagcatg tgggagacaa tgctgtattc
      721 ttttttaaag cacccaccag aaacggtgat tgtaatgata atggtcttct cgatcgagat
      781 gtaaataccc gtttcgttat actgtccgta tcggaaccat gagaactcag ttgcattcca
      841 gccatgctcc agaatatgtg catttttttg cctgatagtt tcttttttta ttcgctcatg
      901 tttaaggcat aacaggcaga taaataatgt cagaaaaaga aagctaataa tgagagcaag
      961 agcgatatag tctgtcacag ggtaatacat attctctccg gtagaagaag ggccgcaaac
     1021 gcggcccggg ctgttatcag aaagagtaac ggaagttggc gttgatcgcg ttatccacgt
     1081 tgtatttacc aaatgccgat ttctcaaact caagaccgaa gcgaagatta tcgcgaattt
     1141 cggcgttgag accaacattc atgagaatac gaccgtcttt ttcacctttg atacgtttct
     1201 caccggacgc atcacgcagt acggtttcac cgttggcaaa caggtcaaac tggtagccaa
     1261 ggccggcgcg ggctgtgact ttccagtcct taccggagaa ggatttaccc acatcaacac
     1321 cggtacgccc aatcagcgga ttaaagtcct tatccttcat ggtcacgctc attccgcgat
     1381 cctgccagtc gaaccgttta cctgatacgg ccccataaac cagttctgcc tgcggctcaa
     1441 tccaggagtc ttctgttaca tgatagcggt agcctgcttc agcacccaca tacaaggaat
     1501 gagaactgta gtcttttgtt ccgagtccag caaaggtcgc cgagtactca ttatcatggt
     1561 gaacatactt actaatcagg tcgatatagg caccagagtc aaatatagcg gaagcataca
     1621 gacctgcccc gacggacttc gtcttgccac tgaatgcatt actgctggca tgactgtcgg
     1681 tatacgtcat agtcagacca gtgaaaaggt caagtccatc cagctcatgt tttttatcca
     1741 caccaatctg cacatgggtg tagttgtcac tgtatccacc agaagctgaa cctgctccgc
     1801 tcatgatgcg ggcccatgca ccggcctcgc cattggtgtc acgcagatcc cccattcgtt
     1861 tattcaggtt gttgacttct gtcaggaagg ctttgtaatt accggacata tacgattttg
     1921 ctgcaccgga agatgctgta ttttcagcca ccttatatcc ggtcagggtc cacgtggtat
     1981 gttcctgatc ctctttcgtg ctgatcacag gagatatgtt gctgaaacct atggcctgag
     2041 tactggcttt aaatatctct ttattgcttc cttttggggc tgttatcagg tcaacgttta
     2101 atcctgagtt attatttccc actttctgta tatcgaccga cagactgtta ttgcttccgg
     2161 ttaactgatt tttaacaatg agtttatccg attgatttac agactggctc agatcagccc
     2221 gcataatcac agcagtatta gtggcatcca gtgtgtttac tgtcacagta tggaaacccg
     2281 aattctctat gttactaaac aacaaaagac tgtcgcgggt ttttaatgat tgcagggctg
     2341 actgtccgtt tactctccat aaggtgttat acatgctggc tgaggctgca ggagcatcaa
     2401 tacctccggt ataagaagta tcaaagccac ccagcaaacc tgtagccagc ggtgtgtgtg
     2461 tggttttgtc caggttatct tctttatact ggtcaacacc aaacagaata gatgcttttt
     2521 tgtctgattt tatattacca ttgacccagg cataattttg agcctttaac gtggcaccat
     2581 cttctgtcat attatatcca tctgacatat agattgccgg gctgtattta ttattttctg
     2641 taggggtgcc tgttaatgtc agggttccgg ccagttcaat agctttatct acatacattt
     2701 ttccctgggc ggtaacagta gctcctttgt tgataatcag gtctgtatta attaaggata
     2761 atgcaccagt catagtaaca gttgatttat caccaacagt tattttgctg ttttgagcac
     2821 tcagactgcc gttgaaacta ctttcgcctg ctgaatcccc ggagcgaagc tgttgtctga
     2881 atccaaaacc ttcgccagta atgtttcttc ctgagaacat gtcaatgtat gcagttttat
     2941 ttcccagaac aatttgagag tttttagcct ctatgtgtcc ttttacatta gcatcccggc
     3001 ctatactcag ggttgcgttt tctaaattta atttccggaa attaaattta cgactttccc
     3061 agtcgggctg ggaaaaagag gctatctgat tgttggactt atattccgta ttgtaacgtt
     3121 tatttacagt gctttcctgg tcctttatgg cagcagaata gtctttttga cataaaaaag
     3181 gaattggaca gttgttattg ccttctttaa atatggcatg ttctgtcgca tgtccttgta
     3241 atgttaactg tgcattccgg acagagatat cgtttttgat atcgactgag ccatcaaaga
     3301 tcagattggc attattgtta tgttgctggg tagtattgat aatatgattt atgcttatat
     3361 tacctgatac attcccatgg tacatatatg catcagtatt agtaagtgat aatgttgatt
     3421 gcttcacgtt tgagttcgtt acggttgttc ccgcatctgt tgctgcgatt ttctgaaatg
     3481 actggtcata gccgtttaga tccagtgttc cgccatttct ggtaaaatag atttcgccat
     3541 taccgctttc gcttaaagcg tctttggcat ttatttttac cgtaccttta cctccggcca
     3601 tgtaaatttt attgaaggct ttttcagcac taaggatgac agtcccatta cctattttaa
     3661 ggttatttcc ctgtgctatt tttacatcca gagtaccact acctatttta tgcagattat
     3721 ctccggcaac gcccttaaca ttccattcaa cggtggtatt attatcaata tctatcccgg
     3781 cacctttata agacttatct tttccgtaca ctttatatgt ctggttctta tcgaatataa
     3841 gcccgccaat gccaagatcg gtatcctgct tgagcgttaa tacccctcct cctgagacag
     3901 acaggtcctt atcttttata atcgatgata actcttgttt tttaccatcc agctgcatag
     3961 tgtttgtgaa attctttttt aaattgttta ccagaatatt attaaataac gagtagtttg
     4021 tccaggctat attggtacta ctgataaaac caactgtact ggtaacacca tggaaaaccc
     4081 atttttgttg ttctttatca tatagataag aaccagaacc actatcgcca aaaattggtg
     4141 agtttttaaa aggggttctg ttggtataaa tgtaactctt gtttttccag tccagattaa
     4201 aaagacttgc gcttaacaag tcgggattat agttttgtcc aaacagatat tgtttgccgt
     4261 ttaatatcgt tgaggttgtt ccggcacctg ctctgaagcc aattatttgt tccttaccgt
     4321 tgtagtttac gccatatctt ttcatcgcat cttcgggaga aagtgagaag tcggcactct
     4381 ccgtaacccc tgttgtttcc acgacgaatt tattcagacg atgaaccgaa aagtcgccag
     4441 atgatactct gttacttgct ttatatgctg tctgatccca tgactgtgtt gttattgcat
     4501 gatgctgtgt accgttatga gtcgcagtaa cactgtacgc acctccaatt gatgttgttg
     4561 cacctttgtt ggaaacagca gaaaaatcag gtacaggtag ttctggaaaa tgaaatatct
     4621 ttccatcttt catcattaat tgaacatttg ttgcacccgg tctgaactct cctctgtttt
     4681 gagcaaggtc aagataatcg cgggtccata tattatttat atccagcatt gctgcaaatg
     4741 ctgtttcgcc ataacttcca tatattgagg ataaaataat accgaggata actttctttc
     4801 ttgatgtacc gacactaact ttccgggtca gttcagaaac agcaactaat ccacctgtaa
     4861 tatgactata tttcagtgaa taaattttat tcatacgtga actccgatat ttctgagttg
     4921 tggttatggg atagcattta attacaacgg ggaactatca gtcatggtac gtaactgata
     4981 agggtataat gataatcact gtcagttata tttgtatgac agatgtaaca aaatgaaaaa
     5041 aaagataagc acaagtgatt gattggttag atattgatca cttggtgttt gtattttgaa
     5101 ttttgcgatc aaaatttgat ctttgttgtt tccggtcaat cctttacagg ttttctgtat
     5161 tatccggggg agttaagtct gtcgatgtcc gacagacttt tgatgatgga aacaacatgc
     5221 agcgataatg tgcatgctgt tttgcaggtg gaactcagaa ggtgaactgg aactgtgcga
     5281 ccgcaccgta agtgttgtcc gtgccgatca ggcctgcaac atcaaggtga accacatcaa
     5341 acggtgagat ccccaccccg gccgtgaatg cactgccgtc gttaccggcc agattctgac
     5401 ggtaaccggc gcgcagctgt gcccatttcc aggcattaaa ttctgtgcca atggcggcaa
     5461 actgacggtt gctgtcggag gtgaaaccgc tggccggggt cagatccaca tcaaatgcag
     5521 tggtgaacat cgcgttgtgc caggagacac cggccgtcac ctgcgggcgg atcctgaagg
     5581 tttccgtgat accgttcact tcttttgtct caatactacg ggagatcagg ttctgtgcga
     5641 ccagtcccag cgtccagttg tcatccaggt ccgtactggc accgatgtcg gcgttgatgc
     5701 catttttcgt gttgtggtag cggtcgcctt tgaaggcgct gctgtcataa tttctgacca
     5761 gcacgttgta gttgaacagg tcgacgcgct ggaatttggg ggggaagccc agagaccatt
     5821 tctgcccggc cgtttccagt tccgtagcca gagcaatacc cacatcagtg atcagtgccg
     5881 tgcgggcaca ggcctgtgat gtcaggcggc ttttgtccac ctcctgcccg gtgctgtttg
     5941 ccacctcttc caggtaagtg aggtcagcat cagagacttt accgtcgaca ctgacggtgc
     6001 cgtgtgattt aaccatcagt gcggcagaga ggcgatcacc gggaagggcc gccacagcag
     6061 aaacgcccag ctgtgcggcg gcatgtgtgt tacggaagtc ccggaggtgt tctttcaggc
     6121 gtgcggcagc ctcaggaaca ccgtggttac tttcgatggc gcggtcgaag gccttccact
     6181 catcactgat ccggtcggcg ttgtcggtga tattatccgg attggagagc tgcgcgccga
     6241 cggacggaag caggatgctg ggctcatgcg cgacgcaaaa tccacgatgt atatatcagt
     6301 actaaaagcg cgacggcgga agaagctctg aaactaatcg gtgaactgta cgccatagag
     6361 cacgatatac gcgggttacc ggtgtctgaa cgcctggcgg tcaggcaaat gcagagtaga
     6421 ccgctcctga cttccctgta taagctgatg caggagaaag aacacacgtt atcgaagaaa
     6481 tgtcgtctgg gagatgcgtt ccggtatatc aggaagcact gggctgcgtt gtgcaacttc
     6541 tgtgatgatg gcctggctga ggtagataat aatgcagcag aaagagcgct tcgtgcagtc
     6601 tgtctcggga agaaaacttt ttcgaggatc actcattccg tgtctgactg cttcagggtt
     6661 taattttgct tctgctgact gaatgatgat tgggcagcaa gtgcaatatc ccacataaat
     6721 ccagtcaact cacgagccac cgccgtaatg gctacttgtg attttttccc ttttgctgtc
     6781 agagttcggt aacgagaaca caatcgttgt tgagctttcc atgcaatatc tttggaatat
     6841 tgtgttacag agtccggtcg gtaatatata agccatgctc caacttttgc aggtgtacga
     6901 taagaccagg cggcttcgta aagcagacgt ctcagttcgc tgtttccaac ttttgttatt
     6961 cctctgggac gaatactgtt tccactggaa tattcacctg gcaccagtct gaggtgtact
     7021 ggcaatagcg gacactacca tttgttcttt ttttaagcag ccatctgatg atatttttcc
     7081 ctgaaggctg ccggggagat attccccaga cgagagtgac gacgctgacg attgtagaaa
     7141 atctcaatgt attcccgtat tactgagatg gcttcatccc ggttattaaa acgatagtgg
     7201 ctcaggctct catttttcag cgttctgagc ctctgattct tccggacaga agataatcat
     7261 gtcggtatga atgttataat taaggactgt gcaataatag ccattccatt cagccatctt
     7321 acgctcgaaa acgatcggta agctggctac tcaccattcc tgctctaaac ctgacgcctc
     7381 tcaagactat tataaatagc ctcaatctca cccttcaggt tatcaccgca ataaaccatt
     7441 gatgtatcgt atcgttcata tcgcgcttcc cgtgtcgcac taaaccagtt gaacgatccc
     7501 acacacagca aaccatcatc accaataaca attttgctat gaacacggtt taccagcttt
     7561 gtagcaatac ccagcgcatt cagtttctcc agcgccgctt taaagttctg ctgcttctct
     7621 tttcgcttct caaaatcatt atgttcagtg ttgtagcttc tgtcagtgac tatcgtgacg
     7681 ttaattccac gtgaacacgc cgcaatcatg gaatcaagaa aaccggtttg ctccagcctt
     7741 tgccaggtca gccatggaga aattatcgtg atgtgtttac tggtattttc aaatgtctga
     7801 ttcaggaaat tatcatgttg ctccacacca tgaagtgtgt agattttggt cccggcggtt
     7861 tttaaatcct tacgctcttt ataatcaaaa gagagcgcat tcttctctga ctcaaagagg
     7921 tattttgcca gtaatccccg tggcgatgag gctggctgga cctcaaacag gtccatatcg
     7981 ccgaagacca gaaaactgtc cttcgcacgg gagactgcaa cattcagcat gctgttatcg
     8041 ctatcaataa acccgccgtc ttcatgtttt gaatagactg gcgagaatat cacaatcgct
     8101 ctttccgctc cctgaagaga gtgcacggtg cccactgtga gcgacttttc attcgtgcct
     8161 gtactgatat cttgtttgcc cagcacctgt ttgatagtgg atacctgagc gctaaaaggc
     8221 gtcacaatac cgacaacttc atgaagcgat ttaccgtaat gcgcttcaat attttgctgg
     8281 ttctctgcca accagaccgc tatcgtttca gcctcaagca aattatatcg acttccacta
     8341 cttgccagct ctcctttacc atcaatatgg agatacccca ttgcgggcat taaattgctc
     8401 tcttcacgcc ctcttttagg caacaactta ccgtgatagc agagcgtatt acagtatcca
     8461 ataatattgt cgtagcaccg gcggtgttca tataggtaca taccacgagc cagttcggga
     8521 tcatattgat agcgcgaagc aaactgcgct attttcataa cgctgccaga tgcggcactt
     8581 ttaccaaggt ctgcgattgc cgtatatttc tcggtaatct cttcttgcgt actgccagac
     8641 agaatttttt ccgccagcat gttaccgaca tcaatcgcag gagcaatact ccatattggc
     8701 gggatctgct ccgtatcgcc aatcactaat gccttcttag ctaatgcaaa cgaggcagca
     8761 gccacttcag gaagcacctg cccggcttca tcgacaatga gtaaatcggc aaaatcatac
     8821 aaataacttt tctcgaattt acgttgtcct ttgtgctcac ttatctgcat attaccgggc
     8881 agcatatagc atgtcatcac cacacatggc gtgagtttca ttcgacgttg ccaacgggcg
     8941 gttacccctt tagcaccttt tttcttcttc tcatcctgca gatcatcaat gctggccata
     9001 tccatcagcc aacgaccttc ccagtagtga gtcgtcagta aaaatgcagg gaagcgaatc
     9061 tgcgtatcag ccagttcatc ggcctgtgag aagctcagtt cctcgtcgcc ctcataccct
     9121 aaatcaaatg ccagcctctg ccactcctga accgcctgct gttctttaag aacgatttca
     9181 tgggcggagt caatctgctg ccggtatgtt gtttgctcgc gctcagcgga attgagcagc
     9241 ccatcaatat tacgttcgat agtttcagga tcagaccact gatttcctgc aatcagcgcg
     9301 cctaatttat cttcgagaaa cagctgtatt tggtactgtc gcttattgcg aaccgccggg
     9361 agccaggaaa ataatgaata gatcagtgat tcaccggcgc gatatttttt ccattccgtt
     9421 ttagcactct tcagtagagt gactttttgt tcttgtccgg aaagtaattt atttaaatta
     9481 tcgagatatt gctcaatatc attagcaata agctcacgcg cagcccgaat ctggcttaac
     9541 gtttgccatg ttgcgttcag tcttatcagt tgctcagatt ttgctgccaa ctgaccatgc
     9601 aagagttcaa tgaccttttc aggggatgaa cactcttttc caggaaaggc tgccttagcc
     9661 ttttccagat aaaacagcag tgcatcctct acatactctt ttgactcaac ctggttgaag
     9721 aaatcttcag tttgatattt tttggctgcc tcagctttac gactgcttga gggaaaataa
     9781 gcaccgaagc ttttcagctc tggcaaccat cgcccggcca tcgcacctga accttgcgaa
     9841 aagtctttcc cgaatgcctc aataatgttc gttacagcct ggttattcgt tgaagtcgcg
     9901 ataataaccg gaggctcaga tttttcgaga gccgctctgg cccactgcgt ggcgatgata
     9961 gaaagcacca gcgtggtttt tccggttccc ggagggccat taacagcaag gatatcgcca
    10021 tgtcttgcat ccagaaaatg gcttaaggca tcgcgctgag cctttgccag cggaaactta
    10081 tctccggagt gtccaagcct gtcgctgaat tttgctcctg gggccagcaa agactctgca
    10141 gcatgaacct ctcgcgaggc gaagcgattg aagagcggca catccttatt gcaaacaagc
    10201 aggtgatcat aaagagaaag gatatgggaa ctggcaccgc cagattgagc cgttttaaca
    10261 atataaccgt gctcagcgag ttcatattgc tcaggttttt caatccagtc gccggcaacg
    10321 ctcttcagta gcctctctga gtcatacaga tattgacgcc actcctgctg caaggcggca
    10381 tatcgtgctt cccgttcttc atccgtttcg gcagtcttat caacgctgtc atcaaagttg
    10441 atagagaacg tggtatgggt cgtcttgtat ttgtcatact gcccaatctc accaatcgaa
    10501 aatgctcctt ttggcaaagg ttcaagcagg tctctgggaa tggaggtcgc tggcgtcgga
    10561 tataaaaaac cctcacggct tagcaaggct ggcgtcacta tcggggtaac aatatcaggc
    10621 gcgcctgcgg aacggtcctt accatgctgc agtaaccgga aataaacttt tgggcgcaag
    10681 atgacatcga ccgtttcgac atcgtctttt tctccctcaa aaaatttact gacaatcgct
    10741 tcgtcaagac gtcccgccgc tatcccatgc cagtgagtga aattttgggc gtctttccgt
    10801 ttaaaactgc cctttcctga ctcagcatcc gcaagcgagt tgcgccagta tgaggcaaac
    10861 cctaaagcat tttcatccat taccagtcct gtgactcaat gaaatccatt gatttatcga
    10921 atataaaatc accgtatgga cagctattgt ccatattgaa aaagttggat gcaatgtttt
    10981 gtttataatt cgatatcaaa tgaactggtt gtacaaggtt cagaacacaa gctcattaaa
    11041 cagtacgtca ggactattga tttcataaca gtgaatacat tttttaccgt ttataataac
    11101 tacctgtgtt ggacggggat accagagcag ccgaaaacac cgggggttat tacctgctct
    11161 catggaaacc atatattcct ggtgctaatc acagtcataa atagcctgaa gaacgtgacg
    11221 aagggacttt aatggaagcc ggagtaactt gtcagaaaca tcaatcttcg tcatcattta
    11281 caaagaaaaa taattagtta aagcagtccg ggctgattgc ccggactgct tactatttta
    11341 atggattgtt aatctttgca tattcaaacg ggctgatacc cttctcccgg ttcgcatcta
    11401 gaaaatcggc ccaccattgc aacatcagtc gccgttcttc cagatgttct gctttatgga
    11461 tatacgcagc acgtacacca ttacgctcct gatggctcat ctgacgttcc acagcatcgc
    11521 gggaccacaa acctgattca atcaatgcac tgcacgccat cgcccggaat ccatggccac
    11581 agacttcctg ggttgtgtca taccccatca ccctcagggc tttatttaca gtattttcac
    11641 tgattggttt acgcgggtca tgtgcacccg taaagatcag accattttca ccagcccatg
    11701 tctgaagttc tgccagtatg gctacaacct gtttgctgag tggcacaaga tgtggtgttc
    11761 gcatcttgga tccccggtgt gagaatttta cgcctggcag aggttcgcgt tcggctggaa
    11821 tagtccacat ggaattgtca atatcaattt ccgaccagcg ggcatatctg agttcactgg
    11881 aacgaataaa aaccagtaaa ttcagcataa ctgccagtcg ggtgacaggc tggcctttat
    11941 aaccgtcaat acgcgacaga agttcaggca ggcgtgaaag ctccagagca gggcgatgct
    12001 ggcgttttac cgtggtcaat gcaccagcca tatccagagc cgggttatat ctgatgatgc
    12061 cagactgtac ggcgtaacgc attacagcag atattctttg ctgcagacga ctggcgattt
    12121 cataaatttg ttttgcttct gcagcacgaa cagggataag caggtccggg gtattgagtg
    12181 ttgtgatatc ccggttgccg ataaagggga aaacgtgtgt ctcgaggctt ttgagtactt
    12241 tatcgctgtg tgattgtgcc cattgtttat tactggcatg ccagcgtcgt gcaatatgtt
    12301 caaaagagag ctgtaaatca gggacaactt ttttaactct tttctgttcg caagggtcaa
    12361 tcccctctga aatcagtttt cgggcctcat cccgttttgt acgagcttcg gagagagaaa
    12421 cttctggata cacaccaagt gccagagtct tctccttacc cagaatacga taacggagac
    12481 gccagtaacg ggaaccatta ggatgaacca gcaggaacat gccggccccg tcagtgagtt
    12541 tataagcctt gtcagtgggc tttgcagccc ggatttttgc gtcagtcagt gccatagttc
    12601 agccttctga atgcgttaaa gagggtacaa attctgtcga actggggtat acccgcaatt
    12661 atacccacac aatggggttg atgtgggggg aatctggttg agttcggtag aattgatttg
    12721 gaggaagaac acttaaatca tggtgttctg aaacgaaaaa cggacctccg tggaggtccg
    12781 ttgatatgaa tttggtgccc ggactcggaa tcgaaccaag gacacgggga ttttcaatcc
    12841 cctgctctac cgactgagct atccgggcaa cggggcgcat taaacctgat tcgcctcgtc
    12901 tcgtcaatca aatttcttca atttactgca gactgcacaa tctatcatca ctttgccgct
    12961 attgcacgca ttgtcaggca aaccaggcgg tccaggcggc ggaaagcggc attgccagaa
    13021 ggagtgctgg aagcatgttg accaccggga acattttaat gccacagatg cgtaatccgg
    13081 ttgccagcag caataaaccg cctacggcgc tgaagtctgc catcatcgac ggtgtgatca
    13141 gcggtaatat cagcgcggca gcccacgcca gcgttaactg gatgatcagt aatgggatac
    13201 taattaccga caccgcaata cccagcgagc aggcgaagat catcgccgta aagaaatcaa
    13261 gaaatgactt ggcgattaaa atactcggat cgccggtcat cccttcgttc atcgccccga
    13321 agatcccggt gccgctggcg caaaacagga caataatcgc gacataattc tgaataaaag
    13381 attcatgcgc tggcttctta cgtgagtgac gaaacagatt ttgtgctttg gcgactgctg
    13441 tattgacacc tttctccagc aaacaaattt cgccaattag agcgccaagt agcgtagcta
    13501 aaaccatcgc tggaaggttg gcgcatttca caaccagtaa aataccaatc cccagcgatg
    13561 ccagaccaaa aatagatgtc atggagacgc ggatacgttc cggtaagcgt tggctgagca
    13621 gtgcgccgag aacgccaccc agtaagactg cactagcgtt gataaaaggg ccgatgacca
    13681 caagagttcc tgttagctgt attgtttgat tttcttatta tgaacttttt gccttgttga
    13741 tggcttgtct ggtcgcaaag tcctggcttg cacgccttag cgaaaggtgc catgattgcg
    13801 cgaattttct cctctctgta cggagtttgc ccgatgcacg ccatctcctt acattctctc
    13861 gcttatcgcc gtttcgcgcg aaacgtttcc ctttctatgt tactgctcat gcggtgaagt
    13921 taacgcacgc tcactgcagg acaacagtaa aatcagagcg tttctgcttt tactgatgtc
    13981 tggcggtcgg agctggtgac cagtttgacc catatctcat ggggcagggt tttccacctt
    14041 gtccggtatt cttacttccc cgaaacgggt ttgcgcttat gaaatcaatg aatattgccg
    14101 ccagtagtga actggtatcc cgactttctt ctcatcgccg cgtggtggcg ttgggagata
    14161 ctgattttac ggacgtcgcg gcagtcgtca ttaccgctgc ggatagtcgc agtggcattc
    14221 ttgcgttgct taagcgcacc ggttttcatc taccggtgtt tttgtattcc gaacatgctg
    14281 ttgaattacc tgcgggcgtt acggcggtaa tcaacggcaa cgagcagcag tggctggagc
    14341 tggaatccgc agcctgtcag tatgaagaga atttgctgcc accgttttat gacacgctga
    14401 cgcagtacgt tgagatgggc aacagcacct ttgcttgccc tggacatcaa catggtgcgt
    14461 tctttaaaaa gcatcctgcc ggacgccatt tttacgattt ctttggtgag aacgtctttc
    14521 gcgccgatat gtgtaacgct gacgtaaaat tgggcgatct gcttattcat gaaggatcgg
    14581 cgaaagatgc gcagaaattc gcagccaaag tctttcatgc cgataaaacc tattttgtgc
    14641 tgaacggcac atcggcagcg aataaagtgg tgacgaatgc gctgttaacg cgtggcgatc
    14701 tggtgctctt cgaccgtaac aaccataagt cgaatcatca cggcgcgctg attcaggcgg
    14761 ggacgacgcc ggtctatctg gaagcttcac gtaacccgtt tggtttcatt ggcggtattg
    14821 atgcgcactg ttttaatgaa gagtatctgc gccagcaaat tcgcgacgtt gcgccagaaa
    14881 aagccgacct gccgcgcccg tttcgcctga cgattattca gctgggaacc tatgacggca
    14941 ctgtctataa cgcccgtcag gtgatcgata ccattgggca tctgtgtgat tacattctgt
    15001 ttgattccgc gtgggtcggt tacgaacagt ttatctcgat gatggcggat agctcgccgc
    15061 tgctgttaga acttaacgaa aacgatccgg ggatttttgt gacccagttg gtgcacaaac
    15121 agcaggctgg attctcacag acgtcgcaga tccataaaaa agataaccat atccgcggac
    15181 aggcgcgttt ttgcccgcat aagcggttga ataacgcctt tatgctccat gcttctacca
    15241 gccctttcta tccgctgttt gctgcactgg atgttaacgc caaaattcat gaaggggaga
    15301 gtgggcgtcg gctgtgggct gagtgcgttg agttgggtat tgagtcgcgc aaggctattc
    15361 ttgcgcgctg taagctgttc cgcccgttta tcccgcccgt tgttgatggc aaattgtggc
    15421 aggattatcc gacatcagtg ttagccagcg accgccgttt tttcagtttt gagccggggg
    15481 cgaagtggca cggctttgaa ggatatgccg cggatcagta ttttgttgat ccgtgcaagc
    15541 tgttactcac tacaccaggt atcaatgccg aaaccggcga atatagcgac tttggcgttc
    15601 cggcgacgat tctggcgcac tatctgcgtg agaacggcat tgtgccggag aagtgcgatc
    15661 tcaactccat tctgttctta ttaactccgg cggaaagcca cgagaagctg gcacaactgg
    15721 tggcgatgct ggcgcaattt gaacagcata ttgaggatga ctcgtcgctg gctgaggtgt
    15781 tgccgagcgt ttataacaaa tatccggtgc gctatcgcga ctacaccctg cgccagttgt
    15841 gtcaggagat gcacgatctg tatgtcagtt tcgacgtcaa agacctacaa aaagcgatgt
    15901 tccgccagca gagtttcccg tcagtggtga tgaaccccca ggatgcgcat agcgcttata
    15961 ttcgcggtga agtggagttg gtgcggattc gtgatgccga agggcgaatt gcggcagaag
    16021 gggcgttgcc ttatccacct ggcgtgcttt gcgtggtacc cggggaagtc tggggtgggg
    16081 cggttcaacg ttatttcctt gcgctggaag aaggcgtgaa tttgttgccg ggattttcgc
    16141 cggagttgca aggtgtctat agcgaaaccg atgcggatgg catgaaacga ttgtacggtt
    16201 atgtgttgaa gtaagaataa aaaaacgggt caccttctgg cgacccgttt ttctttgcgt
    16261 aattagtggc taaccgtctg tgtgcctgtc gggacacgaa cgtgtttata tttgaacatc
    16321 gccatgaacg cgaaggccag aaccacggaa taaccagcga aaatcaacca tacggtctgc
    16381 cagtcggtaa tgccgttttg ggtgtacatc tcaacaactt taccgctcac gatgccgccg
    16441 aggatacagc cgaagccgtt agtcatcatc aggaacatcc cttgtgcact ggcgcgaatt
    16501 gccgggctaa cttctttttc gacaaacacc gaaccagaga tgttgaagaa gtcgaatgcg
    16561 caaccgtaaa cgatcatcga cagtaccagc agtacagtac cgaacggagt cgggtcgccg
    16621 taagcaaaca gcgcaaaacg caggatccac gccacaatac tgatcatcat tacgttctta
    16681 ataccgtagc ggcttaagaa gaacgggatg gtcagaatga acagggtttc agagatctgc
    16741 gaaatcgaca tgatgattga cgcatgctgc acgatgaagc tgctggcaaa catcggatct
    16801 ttgtcgaagc tgtgcaggaa ggtattaccg aacatgttgg taatctgcag ttccgcgccc
    16861 agcagcattg agaagatgaa gaagattgcc atacgcttgt ttttaaacag cgcgaatgca
    16921 tcgaggccca gcagggttgt ccagctctga ttcgcttgct gtttagcaac cggaatatgc
    16981 ggcagagtca gggtaaacag aaccagaatg gcggaaagtg ctgcgccaat atacagctgc
    17041 atgtggctta attcgaagcc agacaggctc accacccaca ttgccatgat aaagccgatg
    17101 gtgccccaga tacggattgg cgggaagtca gtaacgatat ccatcccggc attttgcagg
    17161 cgatagtaag agatggtgtt gattaaccca agcgttggca tataagcaaa cgagttaatc
    17221 aatatcacaa ggaacatcgc ttccggcgtc gtgacctgtg ccgccatgaa cagcgtgata
    17281 gcgccaatgg tgtggcaaat ggcatatacc catttcgcac ttaaccattt gtcggccaca
    17341 atccccagca gcgcaggcat aaataccgcg gcgataccca gtgagctata aactgcgcca
    17401 atagaagcac cgtcaaactt cagggtaaca aacatatagg agccgagggt cgtcagccaa
    17461 cttccccaca gacagaactg cagaaaagag aggattttca gctgcagctt aagattcatg
    17521 ttaatttcct cacatcgtga tgcggatgag tgtttgaaaa agcacatttg accgtgcgag
    17581 atgatgggat tctatcaact tcgcggactg gttttttgtt acctgtggca aataattgca
    17641 aacattccca atttgcaaaa cgaaattgtg atgtggataa catttttgcc cctgagcatc
    17701 gtcaggggcg gttaatggaa ttatcggcgg cggtaggatt tttgcgcggt attcacttta
    17761 taaagataac ggcgagattc cgcagaggga tggcgggtcg tcagcgtctg ataaacatcg
    17821 cccggcgtca tggtgttaat aatattggca gcctgaatct tatcattcga aaagactcgc
    17881 agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg cataacgccg cgatgttggg
    17941 ttatcaattc cgccgagata aacattgttc agcatcgcca gatacgcggt gccggtatca
    18001 atattgctgg caggatcaaa caagaaactg cggctcggcg tgccggattt cccctgcgag
    18061 cggaacacat ctttcccggc agtattttgt accacctgca ttaatcccag cgcatcggaa
    18121 cggctgaccg catacgggtt aaaggaagat tcagtctgca taattgccag aatcagcgac
    18181 tcatcaacgc catatttccg tgacgcctgg cggaccatgc cgagatattt gtgcgcacgt
    18241 ttatcaaggt ggttcggcac catgttaatg gtgacgctgt agatgatacg tagcccgttg
    18301 ctgcggcttt tcagacggtt tttcagcaga taatccgcga agttgcttgc gcgaccttcc
    18361 cagcgaatcg gctgcccggt gttgtccacc acctgaccgt aaaggaaagg ttctttcgaa
    18421 attgtaatat catcaacgtc ggaatagaga tcgaccgaac tcggatcgtc acccatcagc
    18481 aacgttttga taattgcccg gcgcaaatgc gcggcaggtt ctgtcccggc gatggtttcg
    18541 atagtaatcg taccgtcatc gaagttgatg tggctgcggg tctgatattg atcggtgtat
    18601 ttcacgtagt ccttaggacc ggcgatcacc acctctttga agccccagat gttctcaata
    18661 ttgtgggcaa attgccccat cagaatatca aaaccgttgg tatctttgac ccaggcttcg
    18721 ttataggtat cgcctttttt ggtcgtcgaa caggagatga gcaacggcgc aatcaaagcc
    18781 agcgcgagat attttttcat cattccgggt gcgtgttgtg tttgttttta tgcaagctgc
    18841 cggaggcgtg ctccggcact gtttttattt tttatcttcc ggcgtatagc cctcgatatg
    18901 cacctcttta ccctcgaaca ggaagttgac catctcctgc tccagcagct tgcggtgctc
    18961 ggcattcatc atgttgagtt tcttttcatt aatcagcatg gtttgtttgt gctgccactg
    19021 cgcccaggct tctttggaga tctcgttata gatgcgtttt cccagctcgc cggggtacag
    19081 ctgaaaatcc tgaccttctg cttcacgttg caggaaagta caaaaaatcg ttctgctcat
    19141 aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg ccagtgcgta actgctgtaa
    19201 caaacgctcc acgggagccg ccaggccaac tgacggcggt tgcgctaagt tataccagag
    19261 cgcattgcct tcatccatgc agccggtgaa tgacgacacg ggaagccaca taggcacaat
    19321 atctaagtgg aaatggctga aggtatggcg aaacgcggtc agttgcgtca ggttatcggc
    19381 agcaatctgc cgttgtgcca gccaatgccg caaactttct tcgtcggcaa actgcgggaa
    19441 acagtataaa ccgccccaca atccgctcgg cggacgctgc gccagcaata cttcatcttc
    19501 gtgctgtaac aggagaaagt agccggtgcg ctccggcagc gtctgtttcg gttttttgcc
    19561 cggataaagc gaccagctat tgttggtggc ggcaatacat ccgttttgta gcggacagag
    19621 cgaacatttc ggcttcgaac gcgtgcaaat catcgcgccc aaatccatca tcgcctgatt
    19681 aaaccgttcc acgccgaccg cgggcgtcac ctgctcgctc aaactccata gtttattctc
    19741 gacctctttt ttcccaggcc agccgcttac agcatagcag cgcgccagca cgcgtttgac
    19801 gttaccgtcg agaatcggaa agtgcttacc cagagaaagc gagagaatcg cgcctgcggt
    19861 ggaacgcccg acgcccggta acgccgcgac ttcttcaaag gtttccggga atttaccacc
    19921 gtgtaaggtc gccacttgtt gtgccgcttt atgcaggttg cgcgcgcggg cgtaatagcc
    19981 aagcccggtc cacaagtgga gaacttcgtc gagcggcgca ttggcgagat cggtcaccgt
    20041 cgggaagtgc gccataaagc gttcaaaata ggggataacg gtcgcaacct gagtttgttg
    20101 caacatcact tctgagagcc atactttgta gggcgtcttg tcaatttgcc agggcagcgt
    20161 tttccgcccg tatttatcgt accagtccag aacctgggct gaaaattgcg acgcttgcat
    20221 ggtcaccgac ttcactgttg ttgggggcaa gattgcagca cagcgacggc agggtgtaaa
    20281 ccggaacttt ccgcagccac ggccttatca tgcttgcatc cggcaactaa ctttggataa
    20341 tgcccgtttt cagaacactt tcacaagcga ctaaatcttt atgaaaaacg acgtcatttc
    20401 accggaattt gatgaaaacg gccgcccact gcgccgtatc cgtagttttg tgcgccgcca
    20461 ggggcgactg accaaaggcc aggaacatgc gctggaaaac tactggccgg tgatgggcgt
    20521 tgagttcagt gaagatatgc tggatttccc cgcgcttttt ggccgtgaag cgccggtgac
    20581 gcttgagatt ggttttggca tgggggcgtc gctggtggca atggctaaag atcgccccga
    20641 gcaggacttc ctcggcattg aagtgcattc accgggcgtt ggtgcgtgcc tggcttctgc
    20701 gcatgaagaa ggtttaagca acctgcgcgt gatgtgtcac gatgcggttg aagtgctgca
    20761 taaaatgatt cctgacaatt cattgcgcat ggtgcagctc tttttccctg acccgtggca
    20821 caaagcgcgc cataataaac gccgtatcgt tcaggtgccg tttgccgaac tggtaaaaag
    20881 caaactgcag ctggggggcg tattccatat ggcgaccgac tgggaacctt atgcggaaca
    20941 tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac ctgtcagaga gcaatgatta
    21001 cgtaccgcgt ccggcatcac gtccggtgac gaaatttgaa caacgtggtc atcgtcttgg
    21061 tcacggagta tgggacttaa tgttcgagag ggtgaaataa tggcaaagaa ccgtagccgt
    21121 cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat taggattttc ggtggcatgg
    21181 cgattcccgg aaggtacatc ggaagaacag attgataaaa tcgttgatga ctttattaac
    21241 gaggttatcg aaccgaacaa actggccttt gacggcagcg gttatctggc ctgggaaggt
    21301 ctgatctgca tgcaggaaat cggcaaatgc accgaagaac atcaggcgat tgtgcgtaag
    21361 tggctggaag agtgcaaact ggatgaggta cgcaccagcg aacttttcga cgtttggtgg
    21421 gactaagaaa gcatacgggc gatgacaaat gcaaaactgc ctgatgcgct acgcttatca
    21481 ggcctacaga gatgcacgat cgagtaggcg ggataaggcg tttacgccgc atccggcatg
    21541 gaaaacgcgt actttgttat caatctgggg ccagcatatg ctggcctgat ttgttcttga
    21601 gggaagacta tgatgcgcaa aatgctgctg gcggcagcac tttcagtgac ggcaatgacc
    21661 gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg atgtgattgt cagcccgcaa
    21721 accgtgcagg tgaagggcga aaacggcaat ctggtgatca cgccagacgg caacgtgatg
    21781 tataacggta agcaatattc cctgaatgcc gcccagcgcg agcaggcgaa ggattatcag
    21841 gctgaactac gtagcacgct gccgtggatt gatgaaggcg cgaaaagccg cgtcgagaaa
    21901 gcccgtattg cgctggataa aattatcgtt caggagatgg gcgacagcag caaaatgcgc
    21961 agccgtctga ccaaacttga tgcgcagctt aaagagcaga tgaatcgcat tatcgaaacg
    22021 cgcagcgatg gcctgacgtt ccactataaa gccattgatc aggttcgcgc cgaaggccag
    22081 caactagtga atcaggcaat gggcggaatt ttacaggaca gcattaatga aatgggcgcg
    22141 aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg tgctgggaag cttgggcggt
    22201 ctgcaatcct caatccaaac cgagtggaaa aagcaggaaa aagatttcca gcagtttggc
    22261 aaagatgttt gtagccgcgt tgtgactctg gaagatagcc gcaaagccct ggtcgggaat
    22321 ttaaaataat cctctatttt aagacggcat aatacttttt tatgccgttt aattcttcgt
    22381 tttgttacct gcctctaact ttgtagatct ccaaaatata ttcacgttgt aaattgttta
    22441 acgtcaaatt tcccatgcaa agctaaggga taatgcgtag cgtccacgta actggaggaa
    22501 tgaaatggag tttttcaaaa agacggcact tgccgcactg gttatgggtt ttagtggtgc
    22561 agcattggca ttacccaata tcaccatttt agcaaccggc gggaccattg ccggtggtgg
    22621 tgactccgca accaaatcta actacacagc gggtaaagtt ggcgtagaaa atctggttaa
    22681 tgcggtgccg caactaaaag acattgcgaa cgttaaaggc gagcaggtag tgaatatcgg
    22741 ctcccaggac atgaacgata atgtctggct gacactggcg aaaaaaatta acgccgactg
    22801 cgataaaacc gacggcttcg tcattaccca cggtaccgac acgatggaag aaactgctta
    22861 cttcctcgac ctgacggtga aatgcgacaa accggtggtg atggtcggcg caatgcgccc
    22921 gtccacgtcc atgagcgcag acggtccatt caacctgtat aacgcggtag tgaccgcagc
    22981 tgataaagcc tccgctaatc gtggcgtgct ggtggtgatg aacgacaccg tactggacgg
    23041 tcgcgatgtc accaaaacca acaccaccga cgtagcgacc ttcaagtctg ttaactacgg
    23101 tcctctggga tacattcaca acggtaagat tgattaccaa cgtaccccgg cacgtaagca
    23161 caccagcgat acgccattcg atgtctctaa gctgaatgag ctgccgaaag tcggcatcgt
    23221 ttataactac gctaacgcat ccgatcttcc ggctaaagca ctggtagatg cgggctatga
    23281 tggcatcgtt agcgctggtg tgggtaatgg taacctgtat aaatccgtgt tcgacaccct
    23341 ggcaaccgcc gcgaaaaacg gcactgcagt agtgcgttct tcccgcgtac cgacgggtgc
    23401 taccactcag gatgctgaag tggatgatgc gaaatacggc ttcgtcgcct ctggcacgct
    23461 gaacccgcaa aaagcgcgcg tcctgctgca gctggctctg acgcaaacca aagatccgca
    23521 gcagatccag cagattttca atcagtacta atcacctcgt cccggcatcg tgccggggct
    23581 ttttcacttc agacttacgt ccattgccaa ttttcattac cctaatgata atcaccggaa
    23641 taaattattc cgcgcgaggg ttttcgggtg aaaaagcaat ggattgttgg cacggcgctg
    23701 cttatgttga tgactggtaa tgcctgggca gatggcgaac cgccaactga aaatatctta
    23761 aaagatcaat tcaaaaagca gtatcacggc attctcaagc ttgatgccat cagcttaaaa
    23821 aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt
    23881 gacgatctct atacctgggt cggtcagttg gcagattacg aactgctcga acagacctgg
    23941 acgaaagata aaccggtaaa attctcggcg atgttaacca gtaaaggaac gcccgcgtct
    24001 ggctggtcgg tgaactttta ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc
    24061 gacgatatca aaacgaataa taaatatctg atcgtgaata gcgaagattt caattatcgc
    24121 tttagtcagc ttgaatctgc gttgaatacc cagaagaact cgattcctgc gctggaaaag
    24181 gaagtgaaag cgcttgataa gcaaatggtg gcggcgcaga aagcagcgga tgcatattgg
    24241 ggaaaagatg caaacggtaa gcaaatgacc cgcgaagaag cctttaaaaa aattcaccaa
    24301 cagcgtgatg agtttaataa acagaacgat agcgaggcgt tcgcggttaa atatgacaaa
    24361 gaggtttatc aaccggcgat tgcggcatgt cataaacaga gtgaagagtg ttatgaagtg
    24421 ccgattcagc agaaacgaga tttcgatatc aacgaacaac ggcgacagac ttttctgcaa
    24481 tcacaaaaac tcagccgtaa attgcaggat gactgggtaa cgctcgaaaa agggcaatat
    24541 ccgttaacga tgaaagtttc agaaataaac agtaaaaaag tcgcgatttt gatgaaaatt
    24601 gacgatatca accaagctaa tgaacgctag aaaaaagata ccgaacaact gcggcgcaat
    24661 ggggtgatta agtaactcat gttaagtgcc tttgccggat aatataaaaa gttatccggc
    24721 aaattatttt ttacattagc cctagtagct gtggaatgaa caaggatatt tgcggaatgt
    24781 aagtgatcat catcaacgct actagcaatg cagcataaaa tagcagcaat ggtttgatta
    24841 aatgttgaat cttcacaccc gagatagaac aaccaacaaa tagcgcacta cccaccggcg
    24901 gtgttaacag cccaatacaa aggttggcca ccatcataat accgaaatgt actgggtcca
    24961 ttcccagctc ttgtgcaatc ggcaagaaga tcggtgtaaa gattagtaca gccggtgtca
    25021 tgtccataaa gatgccgaca attaataaga cgatattaat cagcagcaat atgatcagtg
    25081 ggttatcaga aatccccatc aatgcgtcgc tgatcatata aggaatatca gcattagtca
    25141 ttgcccagga catgcccacg gaaaaaccga tcaacagtaa tacaatcgaa gtggtaacaa
    25201 ccgactccag aatcagtttg ggtaaatggc gccattttac ttcccggtaa atcagcaccg
    25261 agagaataaa tgtgtagaca acggcaattg ctgacgcttc agttgcggta aaaatgccgc
    25321 ccagaatgcc gcccatcaca atgaacacca gaagcaaact tggtaaggca tccagaaacg
    25381 ctttacaggc ctgagcaaag gttgctcttt ctgaaagcgg gtagccacgg cgtttagcaa
    25441 taatgccgca gactatcatt accgcaagac ccattaagat ccctggcaaa tatcccgcca
    25501 taaacagcga tgctacagaa acgccgccag ctgtcaggga aaacacaatt aacacgtttg
    25561 agggcggtat cagtaatcca gtaatacaag atgagacgtt tacagcggta gaaaatgccg
    25621 gatcataacc ttctttggtc tgaatgggat tcagtgtgcc accaacggca gcagctgccg
    25681 caacggctga accagaaatg gaaccaaaca tcatattcgc caggacgttc acatgaccaa
    25741 gcgaaccagg aacgcggcca accattacct gggcgaggtt aatcaaacgt atcgcaatac
    25801 cgccgctgtt catcagtgtt ccggcaaaga tgaaaaaggg gattgccaac agggcaaagt
    25861 tatccagtcc attagccagt cgttgcgaaa cagtgattgc cgcgatatcc cagggaaaca
    25921 tcaaaagcat tgaagctaca gtggcaatac cgattgagaa cgaaatgggc acaccaataa
    25981 agaccagcac aaaaaaacag ccaaacagtg taatcgcgat ataactatc
//
LOCUS       DAETQL010000057        25703 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.057, whole genome shotgun sequence.
ACCESSION   DAETQL010000057 DAETQL010000000
VERSION     DAETQL010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.057"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..1331
                     /gene="yjeM"
                     /locus_tag="K0W65_002555"
                     /pseudo
     CDS             1..1331
                     /gene="yjeM"
                     /locus_tag="K0W65_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="K0W65_002556"
     CDS             complement(1482..4793)
                     /gene="mscM"
                     /locus_tag="K0W65_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBI3095773.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4815..5783)
                     /gene="psd"
                     /locus_tag="K0W65_002557"
     CDS             complement(4815..5783)
                     /gene="psd"
                     /locus_tag="K0W65_002557"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBI3095774.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="K0W65_002558"
     CDS             complement(5880..6932)
                     /gene="rsgA"
                     /locus_tag="K0W65_002558"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBI3095775.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7027..7572
                     /gene="orn"
                     /locus_tag="K0W65_002559"
     CDS             7027..7572
                     /gene="orn"
                     /locus_tag="K0W65_002559"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBI3095776.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7783..7858
                     /locus_tag="K0W65_002560"
     tRNA            7783..7858
                     /locus_tag="K0W65_002560"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7816..7818,aa:Gly,seq:gcc)
     gene            8016..8091
                     /locus_tag="K0W65_002561"
     tRNA            8016..8091
                     /locus_tag="K0W65_002561"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8049..8051,aa:Gly,seq:gcc)
     gene            8127..8202
                     /locus_tag="K0W65_002562"
     tRNA            8127..8202
                     /locus_tag="K0W65_002562"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8160..8162,aa:Gly,seq:gcc)
     gene            8238..8313
                     /locus_tag="K0W65_002563"
     tRNA            8238..8313
                     /locus_tag="K0W65_002563"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8271..8273,aa:Gly,seq:gcc)
     gene            8547..8600
                     /locus_tag="K0W65_002564"
     CDS             8547..8600
                     /locus_tag="K0W65_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095777.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(8583..9722)
                     /gene="queG"
                     /locus_tag="K0W65_002565"
     CDS             complement(8583..9722)
                     /gene="queG"
                     /locus_tag="K0W65_002565"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBI3095778.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            9721..11268
                     /gene="nnr"
                     /locus_tag="K0W65_002566"
     CDS             9721..11268
                     /gene="nnr"
                     /locus_tag="K0W65_002566"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBI3095779.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKPLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            11240..11701
                     /gene="tsaE"
                     /locus_tag="K0W65_002567"
     CDS             11240..11701
                     /gene="tsaE"
                     /locus_tag="K0W65_002567"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBI3095780.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            11720..13057
                     /gene="amiB"
                     /locus_tag="K0W65_002568"
     CDS             11720..13057
                     /gene="amiB"
                     /locus_tag="K0W65_002568"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBI3095781.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            13067..14914
                     /gene="mutL"
                     /locus_tag="K0W65_002569"
     CDS             13067..14914
                     /gene="mutL"
                     /locus_tag="K0W65_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBI3095782.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            14907..15857
                     /gene="miaA"
                     /locus_tag="K0W65_002570"
     CDS             14907..15857
                     /gene="miaA"
                     /locus_tag="K0W65_002570"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBI3095783.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            15943..16251
                     /gene="hfq"
                     /locus_tag="K0W65_002571"
     CDS             15943..16251
                     /gene="hfq"
                     /locus_tag="K0W65_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBI3095784.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            16327..17607
                     /gene="hflX"
                     /locus_tag="K0W65_002572"
     CDS             16327..17607
                     /gene="hflX"
                     /locus_tag="K0W65_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBI3095785.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            17693..18952
                     /gene="hflK"
                     /locus_tag="K0W65_002573"
     CDS             17693..18952
                     /gene="hflK"
                     /locus_tag="K0W65_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBI3095786.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            18955..19959
                     /gene="hflC"
                     /locus_tag="K0W65_002574"
     CDS             18955..19959
                     /gene="hflC"
                     /locus_tag="K0W65_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBI3095787.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            20041..20238
                     /locus_tag="K0W65_002575"
     CDS             20041..20238
                     /locus_tag="K0W65_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBI3095788.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            20342..21640
                     /gene="purA"
                     /locus_tag="K0W65_002576"
     CDS             20342..21640
                     /gene="purA"
                     /locus_tag="K0W65_002576"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBI3095789.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            21845..22270
                     /gene="nsrR"
                     /locus_tag="K0W65_002577"
     CDS             21845..22270
                     /gene="nsrR"
                     /locus_tag="K0W65_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBI3095790.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            22309..24750
                     /gene="rnr"
                     /locus_tag="K0W65_002578"
     CDS             22309..24750
                     /gene="rnr"
                     /locus_tag="K0W65_002578"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBI3095791.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            24930..25661
                     /gene="rlmB"
                     /locus_tag="K0W65_002579"
     CDS             24930..25661
                     /gene="rlmB"
                     /locus_tag="K0W65_002579"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBI3095792.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT         6019 a         6515 c         7215 g         5954 t
ORIGIN      
        1 ttaatgatgg ctgaaatggg agctgcttat cgcaaagaag aaggtggtat ctattcctgg
       61 atgaataata gtgtcggacc acgttttgcc ttcattggta cgtttatgtg gttttcctct
      121 tatatcatct ggatggtgag tacctccgcg aaagtttggg taccgttctc aacattcctc
      181 tatggtagtg acatgaccca gcactggcgt attgccggac tggagcctac gcaggtggtt
      241 ggtctgctgg cagtagcatg gatgattctg gtcacagtcg ttgcttcaaa ggggattaat
      301 aaaattgccc gcattactgc ggtgggcggt attgcagtaa tgtgtctgaa tttagtattg
      361 ctgttagtaa gcattactat tttgttatta aatggtgggc atttcgcgca ggatattaat
      421 ttccttgcat caccgaaccc gggttatcag tccggtctgg caatgctatc gtttgtggta
      481 tttgctattt ttgcctatgg cggaattgaa gcggttggtg gtctggtcga taaaacggaa
      541 aatccagaaa agaactttgc caaaggtatt gtttttgccg ctattgttat ttcaatcggt
      601 tattcgctgg caatattttt atggggcgtc agcacaaact ggcagcaggt attaagtaat
      661 ggttccgtta accttggcaa tattacctat gtgctgatga agagcctcgg ggtgatgctg
      721 ggtaacgcac tgcatttgtc acctgaagcg tcattgtcgc tgggcgtatg gtttgcgcgt
      781 attaccggac tttcgatgtt cctcgcttat accggtgcgt ttgctattca ccgctgaaag
      841 ccatcatcca ggggacgccg aaagcgttgt ggccggaacc gatgacgcgc ctgaatgcga
      901 tggggatgcc gtctatcgcc atgtggatgc agtgcgggtt ggttactgtc ttcattctgc
      961 tggtttcgtt tggtggcggt accgcatcgg cgttctttaa caagctgacg ctgatggcga
     1021 acgtgtctat gacgcttcct tacctgttcc tcgcgctggc tttcccgttt tttaaagctc
     1081 gtcaggatct cgacagacca tttgtgattt tcaaaacgcg tatgtcggca atgattgcga
     1141 cggtggttgt cgttctggtg gtgacatttg cgaacgtctt caccattatt cagcctgtgg
     1201 ttgaagccgg agactgggac agcacattgt ggatgattgg cggccctgtc ttcttctcgc
     1261 tgttagcgat ggcgatttac cagaactatt gcagtcgcat ggcgaataaa cctgagttag
     1321 ctctcgactg atcatatgaa atcctgcaac tcatcgtctc tccggcgatg agttgtcagg
     1381 aagtaatgcg tatggatgat actctccagg gccggtgata actgaagatg atgttaagct
     1441 gctaaatgag ccacgttgac atcatttggc agcttcatgc attacaaact tcccgcctga
     1501 cgacctttgc ccgcagacgt cagcgttctc cccgtttgtt taccgttgag gctttccaga
     1561 cgcatctgga agggcgggaa tggcatatcg ataccgtggg cgtggaagcc tgccagaatc
     1621 agctggtgga tctcatggcg tagcggcata cggtgaccca tctcagcggc gtaaatacgc
     1681 agctcgaaaa tctgaatccc ctgttgcaga tccaccagga agacttccgg tgccgggttg
     1741 tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga tttccgtcac ttcttcactg
     1801 ttggcatcgg caggggccgg aatcgtcaac actacgcgcg tgaccgagtc agagagcgac
     1861 cagttgataa actgctcggt aataaacgcc ttgttcggca cgattatctc tttacggtcc
     1921 cagtcgctga tggtggtggc gcgggtgtta atttttgtca cgctaccagt aagatcgcgg
     1981 atcgttaccg tatcgccaat gcgaatcggt ttttcgaaca ggatgatcag gccagagata
     2041 aagttggcga aaatttcctg caaaccaaaa ccgagaccaa caccgagcgc ggcaaccagc
     2101 cactgcaatt tcgaccactc aataccaatc attgagaagc cgaccagccc gccaatcagc
     2161 atcagcagat attttgtgat ggtggtgatg gcgtaacccg tacccggcgt taaatccagg
     2221 tgctgcaaaa tcgccagttc cagcagcgcg ggcaggttgc gcaccagctg cgtggtgatg
     2281 ataaatacca gaatggcaat cagcaccgca ccgagggtaa ttggctccag actttctacg
     2341 ccctgtaccg tggaggtgac atcccacagc gaaatatttt cgaggaagcc gaaagcggaa
     2401 tggatttctg accacagcac aatgaccgaa agcagggcga tcaacatcaa aattgagcgc
     2461 accagccgca aggattgcgc actgatggca tcgagatcga cttcgctttc atcgacttca
     2521 attgctcctt ccgggctact gtgatgatgc gcttcctctt cgccacgcgc acgttgcgct
     2581 aacatctctg cccggcgatg cttcgcccgg tcaaacgcca gcctgcgacg ctggatcagc
     2641 atccagcggc ggataacgtg ataaaccacc agtagcagga accagatggc aaccgaggtt
     2701 tcaagcctcg ctaacagtgc ctgcgccgtt gccagataac ccaccgccga cgccagaatg
     2761 gcaaccagtg gtgcgccaat catcatgttc cacagcatat ggttggtaat gttgtcgccg
     2821 ctgccttctt tgttgagata caacggaata ccggcttttt tcaggcttag ggtgaccacc
     2881 gccagcgcac cgcaaatgag gataaagcaa agccgtccca gcgaaccgga aaattcgcgg
     2941 tcgtcgaggt tatcgaacat catcagcgcc ataatcagcc cgatgctcat caggtagtag
     3001 cgcatcccac gggaaacacg ttcgcgcggc cagccaaaat gagcgataaa caagccgttc
     3061 gggcgggcaa aggtggcgca aatcatcact acccacagca gcggcacggt ggccgttaca
     3121 ccatcgccaa tcgcgaccgc cagcggataa ggccacgcct cgcgcaagcc gtaacccagc
     3181 gtcatccaca gcaccggtaa cggtgacgcg acgagaatcg accagaaaag agtgcgcaac
     3241 gtcagccaga agtgatcctg agtcactttg ccgactttcg ccgccgaacg ttcaagaaaa
     3301 cgggtgaaat agcggcgcga gtaaatactg caaccgacca gaatcaacgc gccaaacagc
     3361 ggcaaaattg tctctttgct ggtcagcatc atcacactgg ctttgcccaa ctgactgaag
     3421 gtgtccagcg aaatgagacg acgcagatcc tgggcgattt ccagcggcca ggcgatggtc
     3481 atcgggcgca cgtcagaggc ccagaacaga tagcggtgcg ttgcttcgtt cacctctttc
     3541 agcgcatcct ccagttgcct gttggagact ttcagcttgg tcagttccaa tagtagcgtg
     3601 tcgccaccct gcaacaatga gttcagcaac tcacgctgag tgcgcagttg tgcttccaga
     3661 atacggtttt gctcggcggt cagcggctga ccgtcggcct gatggatttg ccgtagcagc
     3721 ggctgtttat taagtaaatc ctcataacgt aaccgttgca cacgcaactg cgccatttcg
     3781 gtatcaagct gttgtggttt tggcatttct ggtagccgtg ccacctgcgc ccgcagcgct
     3841 tcgccgagca ggttggacga tcccagccat tgcgactgtt cacgcagcgt attcaacgcc
     3901 tgccggacct gtaacgtctg gctggcagcc tgacgctgtt gcgaggcaac gagctccatc
     3961 cgctgcgcct gttgattcag cgctgccgat agttcgcggt taattttgaa ttgcgcgacg
     4021 atatctttcg gcaaatcggc actgttttct gccagtaatt cggtactttc cagcgcccgc
     4081 tccgcctcaa gctgacgttg gctattcaac tgattacgta aggcctgcaa atacgcatcc
     4141 agttgctggc tctctttttc cgccagctct gagcgtaagc gcgctaattc ctggcggtta
     4201 ttggcagaca gctgcgccag ctccagttca tcaacgagcg ccttaagacg tgcagagtca
     4261 gactgcaacg cgaaattttg tgcctgattg agcggagtat tgccggtaag cgttcccagg
     4321 cggcgctcga tctcatttaa ctgacggcgg gcgtcggttt gctgttgcgg cagttgattc
     4381 agcgaatcgg caatctcgcg ggcgcgctcc tgctcttgct gggcctgacg gcttttatcc
     4441 agcaactggc tgctgacctg gagaatttcc tgattcagcg cgtcggtaga cattcccggc
     4501 gacacgctgc gcggctcgtc acgaatgttg tttaattgtg cgcgcagagt agcggagagt
     4561 ttcggataat tatcgataac ttgctgatat tgtttgatgc gctcaaggga accttttcgt
     4621 tcctcaagcg catttaaggc agactggagc gcctctacga cttccggctg tgcgggtttc
     4681 gccgcttttg cctgctccag ttcctgagtg atttgtttgc tatcgggggc cgtcgcggcg
     4741 tacgcccccc aactgaggca ccaggccatc agaaaagtga taatcaggcg cacgtcagcg
     4801 tttcctttga tggattagac ctggtctttt ttatcgtcaa ccaatgggct ggcgtcgtgt
     4861 tctgcttcga tctcttcagc aggaagtggg gcgggttcag cgtctggcgt aacaaaggtt
     4921 tcggtagata ctgccagcgg ctggccaatt ttcgtgacag acaggctttc cagttgctca
     4981 accagattca ctttacccgg tgcaaacagg ttgataacgg tggaaccgag tttaaagcga
     5041 cccatttcct ggcctttcag cagtgccaca gaaccgtcgt tttccccggc aggccaggtc
     5101 cagcgcttga tgataccttc gcgcggcggc gtaatggtgc ccgcccagac cgtctcaatg
     5161 ctgccaacaa tcgtcgctcc gaccagaatc tgcgccattg ggccaaattc ggtatcgaaa
     5221 aggcaaatca cgcgttcgtt acgggcaaac agatttggca cgttctgagc ggtgagatgg
     5281 ttaacggaaa agagatcgcc cggcacgtag atcatctcac gcagaatacc gttgcacggc
     5341 atgtgtacgc gatggtagtc acgcggggag aggtaagtgg tcacaaacgt accgttgcgg
     5401 aacaggtccg ccatcagata gttgcctgcc agcagggctt cgaggctgta gttgtggcct
     5461 ttggcttgca ggattttatc ttcttcgatt ttacccaact ggctgataac gccatcggca
     5521 ggcatgacca gtacattcgg atcggtatcg attgggcgta cttcgtcacg cagcggacgg
     5581 acaaagaatt cgttaaaggt gcggtagctg gcggtgtccg gcttttgcgc ctctttcatg
     5641 tcgaccttgt agtatttaac gaacagatcg ataaccagtt ttgtcagcca tcctgcccgc
     5701 ttgcttgcgc cccaacccgc caggcgagta agccatagtt tcggcagaat gtactgtagc
     5761 gaaagtttaa atgaatttaa caaggtagcc tccaggccat tgttttgtcg ttcctgatcc
     5821 ggccaacatg ccggatcctg aaaaaaaggg gacgattcta acgacggtta gcttaattgt
     5881 cagtcatccg tatcagaaaa gtttttacgc gtttttacct gcgccatgct ttccagaata
     5941 cggtgatagt tttcgaaacg ggtttccgcg attttccctt cctcaaccgc ttcccggata
     6001 gcgcagcccg gatcggtatc gtgtttgcaa tcgcgatatt tacacagacc taaatagtca
     6061 tggaattcga caaagccctg agtgatttgt tccggctcca ggtgccagag gccgaactca
     6121 cgcacaccag gggaatcaat cacatcaccg ccgtgcggga agtgatacag ccgagcggcg
     6181 gtcgtggtgt gctgaccgag ccccgagttg tcagagacat cgttggtcag gatctctttt
     6241 tgcagcccca gcaacgcgtt cagcaggctg gatttaccga cgccagactg cccggcaaaa
     6301 atgctgatgc gcccggtcaa cgcctcttcc agcggtttca gcccatcctg agtatggctg
     6361 gaaaccatca atacgcgata accgatgttg cggtagatat ccatctgctc gttgacgaac
     6421 gccatgcctt cgtcgtccag caggtctatc ttgttgagca cgataatcgg ctcaatctgc
     6481 aaggtttcgc aggccaccag gtaacggtcg ataatattga gcgacagctc cggcaaaatg
     6541 gcggagacaa tgacaatctg gtcgatgttg gcggctatgg gtttcacgcc gtcgtagaaa
     6601 tccggacgcg ttaacaccga ggtgcgctca tgcaccgctt ccacgatccc tttgacattt
     6661 acgccttccg ccgccggttt acccggacgc cagactacgc ggtcgccggt taccagcgaa
     6721 cggatggtac ggcgaatatt gcagcggtga acgtcgccat cggcggattc cacatcagcg
     6781 tgcataccaa agcggctgat gacgatacct tcatcaggct cgccaaacag attgtcgtcg
     6841 tagtcgggct tctccttaga cgttttaaga cgacgctggt gattggcgtt cacgcggcgc
     6901 tgctggcctt tggagagttt atttttactc aatcgtactg actcctggtc gcccctgatg
     6961 ggcaaaacat ctatgataca cgcaattgtg gatcaatata gtcactgtga atgggtggaa
     7021 aatagcatga gtgccaatga aaacaacctg atttggatcg atcttgagat gaccggtctg
     7081 gatcccgagc gcgatcgcat tattgagatt gccacgctgg tgaccgatgc caatctgaat
     7141 attctggcag aagggccgac cattgcagta catcagtccg atgaacagct ggcgctgatg
     7201 gatgactgga acgtgcgcac ccataccgcc agcgggctgg tagagcgcgt gaaagcgagc
     7261 acgatgggcg atcgggaagc tgaactggca acgctcgaat tcttaaaaca gtgggtgcct
     7321 gcgggaaaat cgccgatttg cggtaacagc atcggacagg accgtcgttt cctgtttaaa
     7381 tacatgccgg agctggaagt ctacttccac taccgttatc tcgatgtcag caccctgaaa
     7441 gagctggcgc gccgctggaa gccggaaatt ctggatggtt ttaccaagca ggggacgcat
     7501 caggcgatgg atgatatccg tgaatcggtg gcggagctgg cttactaccg cgagcatttt
     7561 atcaagctgt aaaatttttc ctggtcacgt aagcgccgga atccacttgc ggcggcgtga
     7621 ttttgacgct aaatcgaacg tcttgctgat aatttcagca cttgagataa aaacgcaaaa
     7681 aaaacttttt tggggggttg cagagggaaa gatttctcgt ataatgcgcc tcccgtaacg
     7741 acgcagaaat gcgaaaatta cgaaagcaaa attaagtagt acgcgggaat agctcagttg
     7801 gtagagcacg accttgccaa ggtcggggtc gcgagttcga gtctcgtttc ccgctccaaa
     7861 atttgaaaag tgctgcaaag cacagaccac ccaatcgcag tatttcgaat tgcgacaagg
     7921 cggcaactga acgaatcctt gggaacttac aaaagtatgt gactaaggtg agtgaaggca
     7981 gccaaggcag tagcggttcg aaagacgaag attaagcggg aatagctcag ttggtagagc
     8041 acgaccttgc caaggtcggg gtcgcgagtt cgagtctcgt ttcccgctcc aaaatttaaa
     8101 agtgctgtaa ggcatagacc acccaagcgg gaatagctca gttggtagag cacgaccttg
     8161 ccaaggtcgg ggtcgcgagt tcgagtctcg tttcccgctc caaaatttga aagtgctgta
     8221 aggcacagac cacccaagcg ggaatagctc agttggtaga gcacgacctt gccaaggtcg
     8281 gggtcgcgag ttcgagtctc gtttcccgct ccaaattctt ctctcaataa aatatctaca
     8341 gcgacgcgat gcgttattgc tggtttttgt tgtctctgac aaactcttgt aaacagagtt
     8401 atccacagcc tcaggctgta atcttaattt caaagaaact tcgcacggtg aatggtattt
     8461 ttttaaccta ttgataaata agttaaaaat caagattccg ttttgtcgag tcgatcactt
     8521 gacgatttta ttcgtcttga attgcaatgc gtttttattt ttattcacaa gctgtggatg
     8581 aatcaggcgt cacgcggtaa ccctttttca atcacccgaa ccagacgctg ttttttcggc
     8641 aattgcactt cgactatgca cgcatttcgt ctctctattt gctgcgcaat cgcccacgct
     8701 atgtgctcat cgagaagtgg gtgctcacct ttacgacttt ccagcgctgt caaaatcgtt
     8761 tcatcccaag gggcattgcc taatgccacg gcgatattac gcagccagcg caggtgacct
     8821 atacggcgaa tcgccgatcc ttccgtgact tttaaaaact tctcttcgct ccaggcaaat
     8881 aactcaatga gttccggtgc gtgtagcggc ttacgcgggc tgaaatcgtc ttctgtagtg
     8941 agttgcgaat agcgattcca cgggcagata agctggcagt catcgcaacc gtaaatacgg
     9001 tttcccatta acggtcgcaa ctcttccggg agcgcccctt caagttcgat ggtgagataa
     9061 gagatacagc ggcgagcatc gacggtatat ggctcgacga tggcaccggt cgggcaaatc
     9121 gtcatacagg ccacgcattt gccgcatcct tcctcgactg gttgatccac gggcagcgga
     9181 atatcgacca gcaattcgcc taaaaagaag aacgaaccgg cctcgcgatt gaggataagt
     9241 gagtgcttac ctgtccagcc gagcccagct ttttcagcta acgggcgctc gagaataggc
     9301 gcagaatcga caaacggtct aaaattcagc gaaacacaat gttgctgaat catctcgccc
     9361 agctttttga gtcggttgcg cagaagtttg tgatagtcac ggcccagcgc ataacggcta
     9421 acatagccga gtttggggtt tttcagcgtg ctggcaaatg cggcgttagc aggaaggtaa
     9481 ttcatccgca cgctgattac acgcagcgta ccgggtaata actcatgggg gcgagcgcgc
     9541 agcataccgt gacgtgccat ccagtccatt tcgccgtggt attgtttgtc cagccatgct
     9601 tgcagtttgg gctcggactc gctgagatcg gtatcggtaa tacctacctg ctgaaagccc
     9661 agttccagcc cccactgttt aattttttgc gctaactgat tgagatcgag gggctctgac
     9721 atgacggacc atacaatgaa gaaaaacccc gtaagtatac cacacaccgt ctggtacgcc
     9781 gacgatatcc gccgcggaga acgcgaggcg gcagatgtgc tggggctcac actctatgag
     9841 ctgatgcttc gcgctggcga ggccgcattc caggtgtgtc gttcggcgta tcctgacgcc
     9901 cgccactggc tggtgctgtg cggtcatggt aataacggcg gcgatggcta cgtggtcgcg
     9961 cgactggcca aagcggtcgg cattgaggtc acgttgttgg cccaggagag cgacaaaccg
    10021 ttgccggaag aggccgcgct ggcacgcgaa gcatggttaa acgcgggtgg cgagatccat
    10081 gcttcgaata ttgtctggcc cgaatcggta gatctgattg ttgatgcgct gctcggtacc
    10141 ggcttgcagc aagcgccccg cgaatccatt agccagttaa tcgaccacgc taattcccat
    10201 cctgcgccga ttgtggcggt tgatatccct tccggcctgc tggctgaaac cggcgctacg
    10261 ccaggcgcag tgatcaacgc cgatcacacc atcactttta ttgcgctgaa actaggcttg
    10321 ctcactggaa aagcgcggga tgttacagga caactgcatt ttgactcact ggggctggat
    10381 agctggctgg caggtcagga gacgaaaatt cagcggtttt cggcagaaca actttctcac
    10441 tggctgaaac cgcgtcgccc gacttcgcat aaaggcgatc acgggcggct ggtgattatc
    10501 ggtggcgatc acggcacggc gggggctatt cgtatgacgg gggaagcggc gctacgtgct
    10561 ggtgctggtt tagtccgagt actgacccgc agtgagaaca ttgcgccgct gctgactgca
    10621 cgaccagaat tgatggtgca tgaactgact atggactctc ttaccgaaag cctggaatgg
    10681 gccgatgtgg tggtgattgg tcccggtctg ggccagcaag agtgggggaa aaaagccctg
    10741 caaaaagttg agaattttcg caaaccgatg ttgtgggatg ccgatgcatt gaacctgctg
    10801 gcaatcaatc ccgataagcg tcacaatcgc gtgatcacgc cgcatcctag cgaggccgca
    10861 cggttgttag gctgttccgt cgctgaaatt gaaagtgacc gcttacattg cgccaaacct
    10921 ctggtacaac gttatggcgg cgtagcggtg ctgaaaggtg ccggaaccgt ggtcgccgcc
    10981 catcctgacg ctttaggcat tattgatgcc ggaaatgcag gcatggcgag cggcggcatg
    11041 ggcgatgtgc tctctggtat tattggcgca ttgcttgggc aaaaactgtc gccgtatgat
    11101 gcagcctgtg caggctgtgt cgcgcacggt gcggcagctg acgtactggc ggcgcgtttt
    11161 ggaacgcgcg ggatgctggc aaccgatctc ttttccacgc tacagcgtat tgttaacccg
    11221 gaagtgactg ataaaaacca tgatgaatcg agtaattccg ctccctgatg agcaggcaac
    11281 attagacctg ggcgagcggg tagcgaaagc ctgcgatggc gcaaccgtaa tctatctgta
    11341 tggcgattta ggcgcaggta aaaccacctt tagccggggc tttttacagg ctctgggtca
    11401 tcagggtaat gtcaaaagcc ccacttatac gctggtcgaa ccctatacgc tcgacaactt
    11461 aatggtctat cactttgatt tgtaccgcct tgccgatccc gaggagctgg agtttatggg
    11521 gatccgcgat tattttgcca acgatgccat ctgcctggtg gagtggccac aacaaggtac
    11581 aggtgttctt cctgacccgg atgtcgaaat acacattgat tatcaggcac aaggccgtga
    11641 ggcgcgcgtg agtgcggttt cctctgcggg tgaattgttg ctggcgcgtt tagccggtta
    11701 acctttgaaa ggtggcggga tgatgtatcg catcagaaat tggttggtag cgacgctgct
    11761 gctgctgtgc acgccggtgg gtgccgcgac gctctctgat attcaggttt ctaacggtaa
    11821 tcaacaggcg cggataacgt tgagttttat tggcgatcct gattatgcgt ttagccatca
    11881 aagcaaacgc accgtggcgc tcgatatcaa acaaacgggc atgattcagg gactgccgtt
    11941 gttgttcagc ggcaataatc tggtgaaggc gattcgctct ggaacgccta aagatgcaca
    12001 aacgctacgg ctggtggtcg atcttaccga aaacggtaaa accgaagcgg tgaagcggca
    12061 gaatggcagc aattacactg tcgtctttac gattaacgcc gatgtgccgc caccgcctcc
    12121 tccgccgccc gtggttgcga aacgcgttga aacgcctgcg gttgtcgcac cgcgcgtcag
    12181 cgaaccggcg cgcaatccgt ttaaaacgga aagtaaccgc actacgggtg ttatcagcag
    12241 taatacggta acgcgtccgg cagcgcgcgc gacggctaac actggcgata aaattatcat
    12301 cgctattgat gccggacacg gcggtcagga ccctggcgct atcggccccg gtggtacgcg
    12361 ggagaaaaat gtcaccatcg ccatcgcgcg taaattgcgt actttgctca atgacgatcc
    12421 gatgtttaaa ggcgttttaa cccgtgacgg ggattacttt atctcggtga tggggcgcag
    12481 tgatgtggca cataagcaaa acgccaattt cctcgtgtcg attcacgctg atgccgcacc
    12541 gaaccgtagt gcgactggcg cttccgtatg ggtgctctct aaccgtcgcg ccaacagtga
    12601 aatggccagc tggctggagc agcacgagaa actgtcggag ctgctgggtg gggcgggtga
    12661 tgtgctggcg aacagtcagt ctgaccccta tttaagccag gcggtgctgg atttacagtt
    12721 cggtcattcc cagcgggtag ggtatgatgt agcgaccagt atgatcagtc agttgcaacg
    12781 cattggcgaa atacataaac gtcgaccaga acacgccagc cttggcgttc tgcgctcgcc
    12841 ggatatccca tcagtactgg tcgaaaccgg ttttatcagc aacaacagcg aagaacgttt
    12901 gctggcgagc gacgattacc aacaacagct ggcagaagcc atttacaaag gcctgcgcaa
    12961 ttacttcctt gcgcatccga tgcaatctgc gccgcagggt gcaacggcac aaactgccag
    13021 tacggtgacg acgccagatc gcacgctgcc aaactaagga cgattgatgc caattcaggt
    13081 cttaccgcca caactggcga accagattgc cgcaggtgag gtggtcgagc gacctgcgtc
    13141 ggtagtcaaa gaactagtgg aaaacagcct cgatgcaggt gcgacgcgta tcgatattga
    13201 tatcgaacgc ggtggggcga aacttatccg cattcgtgat aacggctgcg gtatcaaaaa
    13261 agatgagctg gcgctggcgc tggctcgtca tgccaccagt aaaatcgcct ctctggacga
    13321 tctcgaagcc attatcagcc tgggctttcg cggtgaggcg ctggcgagta tcagttcggt
    13381 ttcccgcctg acgctcactt cacgcaccgc agaacagcag gaagcctggc aggcctatgc
    13441 cgaaggacgc gacatggacg tgacggttaa accggcagcg catccggtgg gaacgacgct
    13501 ggaggtgctg gatctgttct acaacacccc ggcgcggcgc aaattcctgc gcaccgagaa
    13561 aaccgaattt agccatattg atgaaatcat ccgccgcatt gcgctggcgc gtttcgacgt
    13621 cacgatcaac ctgtcgcata acggtaaaat tgtgcgtcag taccgcgcag tgccggaagg
    13681 cgggcaaaaa gaacggcgct taggcgcgat ttgcggcacc gcttttctcg aacaagcgct
    13741 ggcgattgaa tggcaacacg gcgatctcac cctgcgcggc tgggtggccg atccaaatca
    13801 cactacgccc gcactggcgg aaattcagta ttgctacgtg aatggtcgca tgatgcgcga
    13861 tcgcctgatc aatcatgcga tccgccaggc ctgcgaagac aaactggggg ccgatcagca
    13921 accggcattt gtgttgtatc tggagatcga cccgcatcag gtggacgtca acgtacaccc
    13981 cgccaaacac gaagtgcgtt tccatcagtc gcgtctggtg catgatttta tctatcaggg
    14041 cgtgctgagc gtgctacaac agcaactgga aacgccgcta ccgctggacg atgaaccaca
    14101 acccgcaccg cgtgccatcc cggaaaaccg cgtggcggcg gggcgcaatc actttgcaga
    14161 accggcagct cgtgagccgg tagctccgcg ctactctcct gcgcccgcct caggcagtcg
    14221 tccggctgcc ccctggccga atgcgcagcc aggctaccag aaacagcaag gtgaagtgta
    14281 tcgccagctt ttgcaaacgc ccgcgccgat gcaaaaacca aaagcgccgg aaccgcagga
    14341 acctgcactt gcggcgaaca gtcagagttt tggtcgggta ctgactatcg tccattccga
    14401 ctgtgcgttg ctggagcgcg atggcaacat ttcactttta tccttgccag tggcagaacg
    14461 ttggctgcgt caggcacaat tgacgccggg tgaagcgccc gtttgcgccc agccgctgct
    14521 gattccgttg cggctaaaag tttctggcga agaaaaatcg gcattagaaa aagcgcagtc
    14581 tgccctagcg gaattgggta ttgatttcca gtcagatgca cagcatgtga ccatcagggc
    14641 cgtgccttta cccttacgcc aacaaaattt acaaatcttg attcctgaac tgataggcta
    14701 cctggcgaag cagtccgtat tcgaacctgg caatattgcg cagtggattg cacgaaatct
    14761 gatgagcgaa catgcgcagt ggtcaatggc acaggccata accctgctgg cggacgtgga
    14821 acggttatgt ccgcaacttg tgaaaacgcc gccgggtggt ctgttacaat ctgttgattt
    14881 acatccggcg ataaaagccc tgaaagatga gtgatatcag taaggcgagc ctgcctaagg
    14941 cgattttttt gatggggccg acggcctccg gtaaaacggc gttagccatt gagctgcgta
    15001 aaattttacc agtagagttg ataagcgttg attctgtcct tatttacaaa gggatggata
    15061 tcgggacggc gaagccgaac gctgaagagt tactcgccgc gccgcaccga ttgctggata
    15121 ttcgcgatcc gtcgcaggct tactcggctg ctgattttcg ccgcgatgcg ctggcggaaa
    15181 tggccgatat caccgcggcg gggcggatcc cactgttagt gggcggtacg atgttgtatt
    15241 tcaaggcatt gctggaaggg ttgtcgccgc taccgtcggc agacccggaa gtgcgggcca
    15301 gaattgagca acaggcggca gagcaaggtt gggagtcatt gcatcgtcaa cttcaggagg
    15361 tagatccggt tgcggcagca aggattcatc caaatgatcc acaaaggctt tcccgggcac
    15421 tggaagtttt tttcatttcg ggtaaaactt taacggaact gacgcaaacg tcaggagacg
    15481 ctctaccgta tcaggtgcat cagttcgcca tcgccccggc gagccgtgaa ctgctccatc
    15541 aacgcattga gcagcgtttt catcagatgt tggcttcagg ttttgaagca gaagtccggg
    15601 cgctttttgc ccgaggagat ttgcatacgg acttgccttc cattcgttgc gtgggttatc
    15661 gccagatgtg gtcttacctt gaaggcgaaa tctcatacga tgaaatggtt tatcgaggtg
    15721 tttgcgccac gagacagttg gcgaagcggc agataacctg gctgcgtggt tgggaagggg
    15781 ttcactggct tgacagtgaa aaaccagaac aggcgcgtga cgaagtatta caggttgttg
    15841 gtgctatcgc aggctgaatg tgtacaattg agacgtatcg tgcgcaattt tttcagaatc
    15901 gaaaggttca aagtacaaat aagcatataa ggaaaagaga gaatggctaa ggggcaatct
    15961 ttacaagatc cgttcctgaa cgcactgcgt cgggaacgtg ttccagtttc tatttatttg
    16021 gtgaatggta ttaagctgca agggcaaatc gagtcttttg atcagttcgt gatcctgttg
    16081 aaaaacacgg tcagccagat ggtttacaag cacgcgattt ctactgttgt cccatctcgc
    16141 ccggtttctc atcacagtaa caacgccggt ggcggtacca gcagtaacta ccatcatggt
    16201 agcagcgcgc agaatacttc cgcgcaacag gacagcgaag aaaccgaata aggtttcggg
    16261 ctgttttttt acacggggag ccagcgatcc tgcgttcccc gctgatctat ttagagggtt
    16321 atacgcttgt ttgaccgtta tgatgctggt gagcaggcgg tactggtaca catctatttt
    16381 acgcaagaca aagatatgga agacctccag gagtttgaat ctctggtctc ttccgccggt
    16441 gtcgaagcat tgcaggtgat taccggtagc cgtaaagcgc cgcacccaaa gtattttgta
    16501 ggtgaaggta aagcagttga aattgcggaa gctgtcaaag cgatgggtgc ttcggtcgtt
    16561 ctttttgacc atgccctgag cccggcgcaa gagcgtaacc tggagcgttt gtgcgagtgt
    16621 cgtgttatcg accgcaccgg ccttatttta gatattttcg cccaacgtgc gcgtacccat
    16681 gagggtaagt tgcaggttga gctggcgcag ctgcgccatc tggctacgcg cctggtgcgt
    16741 ggctggaccc accttgaaag acagaaaggc gggataggtt tgcgtggtcc gggtgaaacc
    16801 cagctcgaaa ccgaccgtcg tttgttgcgt aatcgcatcg tgcagataca gtcgcgcctg
    16861 gaaagagttg aaaagcagcg tgagcagggg cggcaatcgc gtatcaaagc cgacgttcct
    16921 actgtttcgc tggtgggata taccaacgcc ggtaaatcta cccttttcaa tcgcatcacc
    16981 gaagcgcggg tctacgcggc agaccagttg tttgccaccc tcgacccgac gttgcggcgt
    17041 attgacgttg cagatgtcgg tgaaaccgta cttgcagata ccgtagggtt tattcgccac
    17101 ctgccgcacg atctggtggc ggcatttaaa gccacgttac aagagacgcg gcaagccaca
    17161 ttactgctgc acgtcattga tgcggcggat gtgcgtgtac aagaaaacat cgaagcggtg
    17221 aatacggttc ttgaagagat cgacgctcac gagatcccaa ccctgctggt gatgaacaag
    17281 atcgatatgc tggaagattt cgaaccgcgt attgatcggg acgaagagaa caaaccgatc
    17341 cgtgtctggc tttccgcaca gaccggagcg gggataccac agctttttca ggctttgacg
    17401 gagcggcttt ccggcgaggt ggcgcagcat acattgcgtc tgccaccgca ggaagggcgt
    17461 ctgaggagtc gtttttatca gcttcaggca atagaaaaag agtggatgga ggaggacggc
    17521 agcgtaagtc tgcaagttcg tatgccgatc gttgactggc gtcgcctctg taaacaagaa
    17581 ccggcgttga tcgattacct gatctaacgg cgtagcgtct gaagcgtgga gtcatatcct
    17641 ctggcgtcga aagacaacag ggatcaccgc ataacaaata tggagcacaa acatggcgtg
    17701 gaatcagccc ggtaataacg gacaagaccg cgacccgtgg ggaagcagca aacctggcgg
    17761 caactctgag ggaaatggaa acaaaggcgg tcgcgatcaa gggccacctg atttagatga
    17821 tatcttccgc aaactgagca aaaagctcgg tggtctgggc ggcggtaaag gcaccggatc
    17881 tggcggtggc agttcatcac aaggcccgcg cccgcagctt ggcggtcgtg tcgttaccat
    17941 cgcagcggca gcgattgtca ttatctgggc ggccagtggt ttctatacca ttaaagaagc
    18001 cgaacgcggc gtggtaacac gctttggtaa attcagccat ctggttgagc cgggtctgaa
    18061 ctggaaaccg acgtttatcg acgaagtcaa accggtgaac gtggaagccg tgcgtgaact
    18121 ggccgcttct ggtgtgatgc tgacgtcgga cgagaacgta gtgcgcgttg agatgaacgt
    18181 gcagtaccgc gtcaccaatc cggaaaaata tctgtatagc gtgaccagcc cggatgacag
    18241 cctgcgtcag gctaccgaca gcgccctgcg tggagttatc ggtaaataca ccatggaccg
    18301 cattctgacg gaaggtcgta ccgtgattcg tagcgatact cagcgcgaac tggaagagac
    18361 gattcgtccg tatgacatgg gtatcacgct gctggacgtc aacttccagg ctgctcgtcc
    18421 gccggaagaa gtaaaagcgg cgtttgacga tgcgattgcc gcgcgtgaaa acgaacagca
    18481 atacattcgt gaagcagaag cgtattccaa cgaagttcag ccgcgtgcga acggtcaggc
    18541 gcaacgtatc ctcgaagagg cgcgtgcgta caaggcccag accatcctgg aagctcaggg
    18601 tgaagtggcg cgctttgcta aacttctgcc ggaatataaa gccgcgccgg aaattactcg
    18661 cgagcgtctg tatatcgaga cgatggaaaa agtgttgggt aacacccgca aagtgctggt
    18721 taacgataaa ggtggcaacc tgatggttct gccgttagac cagatgctga aaggtggtaa
    18781 cgcccctgcg gcgaagagcg ataacggtgc cagcaatctg ctgcgtctgc cgccagcctc
    18841 ttcctccaca accagtggag caagcaacac gtcgtccacc agtcagggcg atattatgga
    18901 ccaacgccgc gccaacgcgc agcgtaacga ctaccagcgt cagggggaat aacgatgcgt
    18961 aagtcagtta tcgcgattat catcatcgtg ctggtagtgc tttacatgtc tgtctttgtc
    19021 gtcaaagaag gtgagcgcgg tattacgctg cgttttggta aggtactgcg tgacgatgac
    19081 aacaaacctt tggtttatga gcctggtctg catttcaaga taccgttcat tgaaacggtg
    19141 aaaatgctcg acgcacgtat tcagaccatg gacaaccagg ccgaccgctt tgtgaccaaa
    19201 gagaagaaag acctgatcgt cgactcttac atcaaatggc gcatcagcga tttcagccgt
    19261 tactacctgg caacgggtgg tggcgacatt tcgcaagcgg aagtgctgtt gaaacgtaag
    19321 ttctctgacc gtctgcgttc tgaaattggt cgcctggacg tgaaagatat cgtcaccgat
    19381 tcccgtggtc gtctgaccct cgaagtacgt gacgcgctga actccggttc tgcgggtaca
    19441 gaagatgaag ttactacccc ggcagcagat aacgccattg ccgaagcggc agagcgcgta
    19501 acggctgaga cgaagggcaa agttccggtc atcaacccga acagtatggc ggcgctgggt
    19561 attgaagttg tcgatgtgcg tatcaagcag atcaacctgc cgaccgaagt gtctgaagcg
    19621 atctacaacc gtatgcgcgc cgagcgtgaa gcggtagcgc gtcgtcaccg ttcacaaggt
    19681 caggaagaag cggaaaaact gcgcgcgact gccgactatg aagtgaccag aacgctggca
    19741 gaagctgagc gtcagggccg cattatgcgt ggtgaaggcg atgccgaagc agccaaactg
    19801 tttgctgatg cattcagtaa agatccggac ttctacgcat tcatccgtag cctgcgtgct
    19861 tatgagaaca gcttctctgg caatcaggac gtgatggtca tgagcccgga tagcgatttc
    19921 ttccgctaca tgaagacgcc gacttccgca acgcgttaat ataacgactg cggtacaggt
    19981 caataaagcc accgcatcct cagggatgtc ggtggttttc tttttctata aggataatga
    20041 atgaattcga caatctggct ggcgcttgcc ctggttttgg tactggaagg tttagggccg
    20101 atgctttacc cgaaggcatg gaagaagatg atctctgcga tgaccaattt gcccgataat
    20161 attttacgtc gttttggcgg tggacttgtg gttgcgggcg ttgtggtcta ctacatgttg
    20221 aggaaaacga ttggctgaac aaaaaacaga ctgatcgagg tcatttttga gtgcaaaaag
    20281 tgctgtaact ctgaaaaagc gatggtagaa tccattttta agcaaacggt gattttgaaa
    20341 aatgggtaac aacgtcgtcg tactgggcac ccaatggggt gacgaaggta aaggtaagat
    20401 cgtcgatctt ctgactgaac gggctaaata tgttgtacgc taccagggcg gtcacaacgc
    20461 aggccatact ctcgtaatca acggtgaaaa aaccgttctc catcttattc catcaggtat
    20521 tctccgcgag aatgtaacca gcatcatcgg taacggtgtt gtgctgtctc cggccgcgct
    20581 gatgaaagag atgaaagaac tggaagaccg tggcatcccc gttcgtgagc gtctgctgct
    20641 gtctgaagca tgtccgctga tccttgatta tcacgttgct ctggataacg cgcgtgagaa
    20701 agcgcgtggc gcgaaagcga tcggcaccac cggtcgtggt atcgggcctg cttatgaaga
    20761 taaagtggca cgtcgcggtc tgcgtgttgg cgaccttttc gacaaagaaa ccttcgctga
    20821 aaaactgaaa gaagtgatgg aatatcacaa cttccagttg gttaactact acaaagctga
    20881 agcggttgat taccagaaag ttctggatga tacgatggct gttgccgaca tcctgacttc
    20941 tatggtggtt gacgtttctg acctgctcga ccaggcgcgt cagcgtggcg atttcgtcat
    21001 gttcgaaggt gcgcagggta cgctgctgga tatcgaccac ggtacttatc cgtacgtaac
    21061 ttcttccaac accactgctg gtggcgtggc gaccggttcc ggcctgggcc cgcgttatgt
    21121 tgattacgtt ctgggtatcc tcaaagctta ctcaactcgt gtaggtgcag gtcctttccc
    21181 ggccgaactg tttgatgaaa ctggcgagtt cctctgcaag cagggtaacg aattcggtgc
    21241 aactacgggt cgtcgtcgtc gtaccggctg gctggacacc gttgccgttc gtcgtgcggt
    21301 acagctgaac tccctgtctg gcttctgcct gactaaactg gacgttctgg atggcctgaa
    21361 agaggttaaa ctctgcgtgg cttaccgtat gccggatggt cgcgaagtga ctaccactcc
    21421 gctggcagct gacgactgga aaggtgtaga gccgatttac gaaaccatgc cgggctggtc
    21481 tgaatccact ttcggcgtga aagatcgtag cggcctgccg caggcggcgc tgaactacat
    21541 caagcgtatt gaagagctga ccggtgtgcc gatcgatatc atctctaccg gtccggatcg
    21601 tactgaaacc atgattctgc gcgacccgtt cgacgcgtaa ttctggtacg cctggcagat
    21661 attttgcctg ccgggctaac agtgtgatac attgctgtgt cggttaagcc attacgctat
    21721 ccgacacagt gttaaatcct cgcttttttc cttccccgaa ctgaaataaa ttagcgacac
    21781 agcttgtggc tggtttatca tcaatataaa tgtatttttt cccgatttcc cttttgaggt
    21841 tgatgtgcag ttaacgagtt tcactgatta cggattacgt gcgctgatct acatggcgtc
    21901 attgccagaa gggcggatga ccagtatttc tgaagtgact gacgtctacg gcgtctcccg
    21961 taatcatatg gtcaaaataa tcaatcaact tagtcgtgcc ggctacgtga ctgctgttcg
    22021 tggaaaaaat ggcggcattc gcctgggtaa accggcgagt gcgatacgta ttggtgatgt
    22081 ggtgcgcgag ctggagccct tatcgctggt gaattgcagc agtgagtttt gccacattac
    22141 acctgcctgt cggttgaaac aggcgctttc taaggccgtg caaagttttc ttacggaact
    22201 ggataactac acgcttgccg atttggttga agagaatcaa ccgctttata aattattgct
    22261 ggtggagtga cgaaaatctt catcagagat gacaacggag gaaccgagat gtcacaagat
    22321 cctttccagg aacgcgaagc tgaaaaatac gcgaatccca tccctagtcg ggaatttatc
    22381 ctcgaacatt taaccaaacg tgaaaaaccg gccagccgtg atgagctggc ggtagaactg
    22441 cacattgaag gcgaagagca gcttgaaggc ctgcgtcgcc gcctgcgcgc gatggagcgc
    22501 gatggtcaac tggtcttcac tcgtcgtcag tgctatgcgc tgccggaacg cctcgacctg
    22561 gtgaaaggta ccgttattgg tcaccgtgat ggctacggct ttctgcgggt tgaagggcgt
    22621 aaagatgatt tgtatctctc cagcgagcag atgaaaacct gcattcatgg cgatcaggtg
    22681 ctggcgcagc cgctgggtgc tgaccgtaaa ggtcgtcgtg aagcgcgtat tgtccgcgta
    22741 ctggtgccaa aaaccagcca gattgttggt cgctacttta ccgaagcggg cgtcggcttt
    22801 gtggttcctg acgatagccg tctgagcttc gatatcttaa tcccgcccga tcagatcatg
    22861 ggcgcgcgga tgggctttgt ggtcgtagtc gaactgactc agcgtccgac tcgccgcacc
    22921 aaagcggtgg gtaaaatcgt cgaagtgctg ggcgacaata tgggcaccgg catggcggtt
    22981 gatatcgctc tgcgtaccca tgaaattccg tatatctggc cgcaggctgt tgagcaacag
    23041 gttgcggggc tgaaagaaga agtgccggaa gaagcaaaag cgggccgtgt cgatttgcgc
    23101 gatttaccgc tggtcaccat tgatggcgaa gacgcccgtg actttgacga tgcagtttac
    23161 tgcgagaaaa aacgcggcgg cggctggcgt ttatgggtcg cgattgccga cgtcagctac
    23221 tatgtgcgtc cgccaacgcc gctggacaga gaagcgcgta accgtggcac gtcggtgtac
    23281 ttcccttcgc aggttatccc gatgctgccg gaagtgctct ctaacggcct gtgttcgctc
    23341 aacccgcagg tagaccgcct gtgtatggtg tgcgagatga cggtttcgtc gaaaggccgc
    23401 ctgacgggct acaaattcta cgaagcggtg atgagctctc acgcgcgtct gacctacacc
    23461 aaagtctggc atattctgca gggcgatcag gatctgcgcg agcagtacgc cccgctggtt
    23521 aagcatctcg aagagttgca taacctctat aaagtgctgg ataaagcccg tgaagaacgc
    23581 ggtgggatct catttgagag cgaagaagcg aagttcattt tcaacgctga acgccgtatt
    23641 gaacgtatcg aacagaccca gcgtaacgac gcgcacaaat taattgaaga gtgcatgatt
    23701 ctggcgaata tctcggcggc gcgtttcgtt gagaaagcga aagaaccggc actgttccgt
    23761 attcacgaca agccgagcac cgaagcgatt acctctttcc gttcagtgct ggcggagctg
    23821 gggctggagc tgccgggtgg taacaagccg gaaccgcgtg actacgcgga actgctggag
    23881 tcggttgccg accgtcctga tgcagaaatg ctgcaaacca tgctgctacg ctcgatgaaa
    23941 caggcgattt acgatccaga aaaccgtggt cacttcggtc tggcattgca gtcctatgcg
    24001 cactttactt cgccgattcg tcgttatcct gacctgacgc tgcaccgcgc cattaaatat
    24061 ctgctggcga aagagcaggg gcatcagggc aacaccactg aaaccggcgg ctaccattat
    24121 tcgatggaag agatgttgca actgggtcag cactgttcga tggcggaacg tcgtgccgac
    24181 gaagcaacgc gcgatgtcgc tgactggctg aagtgtgact tcatgctcga ccaggtaggt
    24241 aacgtcttta aaggcgtaat ttccagcgtc actggctttg gcttcttcgt ccgtctggac
    24301 gacttgttca ttgatggtct ggtccatgtc tcttcgctgg acaatgacta ctatcgcttt
    24361 gaccaggtag ggcaacgcct gatgggggaa tccagcggcc agacttatcg cctgggcgat
    24421 cgcgtggaag ttcgcgtcga agcggttaat atggacgagc gcaaaatcga ctttagtctg
    24481 atctccagtg aacgcgcacc gcgcaacgtc ggtaaaacgg cgcgcgagaa agcgaaaaaa
    24541 ggcgatgcag gcaaaaaagg cggcaagcgt tgtcaggtcg gtaaaaaggt aaactttgag
    24601 ccagacagcg ccttccgcgg tgagaaaaaa acgaagccga aagcggcgaa gaaagacgcg
    24661 agaaaagcga aaaagccatc ggcgaaaacg cagaaaatag ccgcagcgac caaagcgaag
    24721 cgtgcggcga agaaaaaagt ggcagagtga tcaataccct ctttaaaaga agagggttag
    24781 attgctgaca aaatgcgctt tgttcatgcc ggatgcggcg tgaacgcctt atccggccta
    24841 cataatcacg caaattcaat atattgcaga gatcatgtag gccggataaa cgtagtgcat
    24901 caggcaaaac gtaaacaacg agtacattaa tgagcgaaat gatttacggc atccacgcag
    24961 tgcaggccct gctggagcgc gcccctgaac gttttcagga agtctttatt ttaaaaggcc
    25021 gtgaagataa acgtctgtta ccgctgattc acgcccttga gtctcagggc gtggttatcc
    25081 agttggcaaa ccgccaatat ctcgacgaga aaagcgacgg tgccgtgcat cagggcatta
    25141 tcgcccgcgt gaagccagga cgtcagtatc aggaaaacga tctgccggat ctgatcgctt
    25201 cgctcgatca accgttcctg ctgatcctcg acggcgtaac cgatccgcac aacctcggcg
    25261 cgtgcctgcg tagtgcggac gccgcaggcg ttcatgcggt gattgtgccg aaagatcgct
    25321 ccgcacagct caacgctacg gcgaaaaaag tagcctgcgg cgcggcagaa agcgttccgc
    25381 tgattcgggt gactaacctt gcgcgcacca tgcgtatgtt gcaggaagag aatatctgga
    25441 tcgtcggtac ggcaggcgag gcggatcata cactctatca gagcaaaatg accggacgcc
    25501 tggcgctggt gatgggcgcg gaaggtgaag gtatgcgtcg cctgacccgt gaacattgcg
    25561 atgagttgat cagcatccca atggcaggaa gtgtttcttc cctgaacgtt tcggttgcaa
    25621 ccggaatttg cttatttgaa gcggttcgtc agcgcagcta gttcctctga attatgaaaa
    25681 gccatccaga tttggatggt ttt
//
LOCUS       DAETQL010000058        25663 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.058, whole genome shotgun sequence.
ACCESSION   DAETQL010000058 DAETQL010000000
VERSION     DAETQL010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25663
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.058"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="K0W65_002580"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="K0W65_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HBI3095793.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="K0W65_002581"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="K0W65_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HBI3095794.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="K0W65_002582"
     CDS             2630..3391
                     /locus_tag="K0W65_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBI3095795.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="K0W65_002583"
     CDS             complement(3442..4326)
                     /locus_tag="K0W65_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBI3095796.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="K0W65_002584"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="K0W65_002584"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBI3095797.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="K0W65_002585"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="K0W65_002585"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBI3095798.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="K0W65_002586"
     CDS             6202..6474
                     /locus_tag="K0W65_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBI3095799.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="K0W65_002587"
     CDS             6844..7920
                     /locus_tag="K0W65_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBI3095800.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="K0W65_002588"
     CDS             7947..9326
                     /locus_tag="K0W65_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3095801.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="K0W65_002589"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="K0W65_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="K0W65_002590"
     CDS             10409..11245
                     /locus_tag="K0W65_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBI3095802.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="K0W65_002591"
     CDS             11250..12197
                     /locus_tag="K0W65_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HBI3095803.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="K0W65_002592"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="K0W65_002592"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HBI3095804.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="K0W65_002593"
     CDS             13324..14082
                     /locus_tag="K0W65_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI3095805.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="K0W65_002594"
     CDS             14200..15558
                     /locus_tag="K0W65_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3095806.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="K0W65_002595"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="K0W65_002595"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBI3095807.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="K0W65_002596"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="K0W65_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBI3095808.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="K0W65_002597"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="K0W65_002597"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBI3095809.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="K0W65_002598"
     CDS             19503..20054
                     /locus_tag="K0W65_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBI3095810.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="K0W65_002599"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="K0W65_002599"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBI3095811.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="K0W65_002600"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="K0W65_002600"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBI3095812.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="K0W65_002601"
     CDS             23307..24347
                     /locus_tag="K0W65_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBI3095813.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..>25663)
                     /gene="gdhA"
                     /locus_tag="K0W65_002602"
     CDS             complement(24464..>25663)
                     /gene="gdhA"
                     /locus_tag="K0W65_002602"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBI3095814.1"
                     /translation="SLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYK
                     GGMRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTL
                     MTELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRP
                     EATGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSG
                     TVVDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQ
                     NELDVDAAHQLIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGL
                     EMAQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADA
                     MLAQGVI"
BASE COUNT         6519 a         5971 c         6625 g         6548 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctagctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc ggcgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaag ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatttgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgccg ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 acattgcttc tgtgaaataa accagaccgt agccggtagc ttccgggcga ataagactac
    25201 cgccaaatga aaggccctta ccggtgaaga cgcaggcggt attgttggag agctttttca
    25261 tcatccccgc cataaagccg acttcacgac caccaacgcc gatatcacct gccggaacgt
    25321 cggtatccgc gcccaggtgg cgatacagtt cagtcatcag cgtctggcaa aaacgcatca
    25381 ctttaccttc gctttttcct ttcggatcga aatcgctgcc gcctttacca ccgcccatcg
    25441 gcagggtagt cagggcattt ttgaaggttt gttcaaagcc gaggaatttg agaatggaaa
    25501 ggttaactga cggatggaag cgcataccgc ctttgtacgg gccgatggca gagctgaact
    25561 gcacacgcca tgcacggttg acctgtatct ggttgcgatc atcaacccat accacgcgaa
    25621 actggatcac gcgctccggt tcaaccagac gctccagtaa tga
//
LOCUS       DAETQL010000059        24800 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.059, whole genome shotgun sequence.
ACCESSION   DAETQL010000059 DAETQL010000000
VERSION     DAETQL010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24800
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.059"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..386)
                     /locus_tag="K0W65_002603"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="K0W65_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="K0W65_002604"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="K0W65_002604"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBI3095815.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="K0W65_002605"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="K0W65_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBI3095816.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="K0W65_002606"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="K0W65_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBI3095817.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="K0W65_002607"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="K0W65_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBI3095818.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="K0W65_002608"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="K0W65_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HBI3095819.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="K0W65_002609"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="K0W65_002609"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBI3095820.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="K0W65_002610"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="K0W65_002610"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBI3095821.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="K0W65_002611"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="K0W65_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBI3095822.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="K0W65_002612"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="K0W65_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBI3095823.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="K0W65_002613"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="K0W65_002613"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBI3095824.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="K0W65_002614"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="K0W65_002614"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBI3095825.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="K0W65_002615"
     CDS             10748..11212
                     /locus_tag="K0W65_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI3095826.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="K0W65_002616"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="K0W65_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HBI3095827.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFVWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /locus_tag="K0W65_002617"
     CDS             12236..13672
                     /locus_tag="K0W65_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBI3095828.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="K0W65_002618"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="K0W65_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBI3095829.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="K0W65_002619"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="K0W65_002619"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBI3095830.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="K0W65_002620"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="K0W65_002620"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBI3095831.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="K0W65_002621"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="K0W65_002621"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBI3095832.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="K0W65_002622"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="K0W65_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HBI3095833.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="K0W65_002623"
     CDS             complement(20505..20798)
                     /locus_tag="K0W65_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBI3095834.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="K0W65_002624"
     CDS             complement(20795..22084)
                     /locus_tag="K0W65_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBI3095835.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="K0W65_002625"
     CDS             complement(22140..23078)
                     /locus_tag="K0W65_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HBI3095836.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="K0W65_002626"
     CDS             complement(23098..23862)
                     /locus_tag="K0W65_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HBI3095837.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            24182..24682
                     /locus_tag="K0W65_002627"
     CDS             24182..24682
                     /locus_tag="K0W65_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBI3095838.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLAIL
                     VMLPTY"
     gene            24716..>24800
                     /locus_tag="K0W65_002628"
     CDS             24716..>24800
                     /locus_tag="K0W65_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3095839.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQ"
BASE COUNT         6134 a         5983 c         6522 g         6161 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgtctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaatagtgtg cgatagcggt
    23941 gcgggggggg ggggcgacgg agcaagctgt tttccttgcc aacaatcttg caaaaaaaat
    24001 gcgattgcta aaacaaaagt gcaatttgcg gcaatatagt tattccatga ccgtacaatc
    24061 ttgcattcag tgtaaataat cttgtttagc tctcgctaaa cgcggttgta taaccgcaat
    24121 acacgccgta ttttatccgg catatattgt ttttgcttgc tcatggaata gggaggttgt
    24181 catgtatcaa cgctgttttg ataatgccag tgaaacgctg tttgtcgccg gtaaaacgcc
    24241 acggctttca cgttttgcat ttagcgatga tccaaaatgg gagtctggac atcacgttca
    24301 tgacaatgaa accgagctga tttacgtcaa gaaaggggtt gcaaggttta ccatcgattc
    24361 ttcgttatat gtcgcacatg cggatgacat tgtggtgata gaacgcggca ggctgcatgc
    24421 ggtggcctct gacgttaacg atccggcaac gacgtgtacc tgtgcgctgt acggctttca
    24481 gtttcagggg gctgaggaaa atcagttact gcaaccgcat tcttgtccgg taattgccgc
    24541 ggggcaggga aaagaagtca taaaaacctt atttaatgag ctaagtgtga ttttgccgca
    24601 aagtaaaaat agccaaacat cttcgttatg ggatgcatta gcctatacat tagcaattct
    24661 ggtaatgctg ccaacttact gatttagtgt atgatggtga ttttaaggtg cttgcgtggc
    24721 ttccatttcc atcagatgtc cttcctgctc cgctactgaa ggcgtggtgc gtaacggcaa
    24781 aagcactgcc ggacatcagc
//
LOCUS       DAETQL010000060        24453 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.060, whole genome shotgun sequence.
ACCESSION   DAETQL010000060 DAETQL010000000
VERSION     DAETQL010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24453
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.060"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..202)
                     /locus_tag="K0W65_002629"
     CDS             complement(<1..202)
                     /locus_tag="K0W65_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HBI3095840.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLAPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGM"
     gene            complement(295..867)
                     /locus_tag="K0W65_002630"
     CDS             complement(295..867)
                     /locus_tag="K0W65_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HBI3095841.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            1085..1945
                     /gene="tesB"
                     /locus_tag="K0W65_002631"
     CDS             1085..1945
                     /gene="tesB"
                     /locus_tag="K0W65_002631"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HBI3095842.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1994..3280)
                     /gene="amtB"
                     /locus_tag="K0W65_002632"
     CDS             complement(1994..3280)
                     /gene="amtB"
                     /locus_tag="K0W65_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBI3095843.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3310..3648)
                     /gene="glnK"
                     /locus_tag="K0W65_002633"
     CDS             complement(3310..3648)
                     /gene="glnK"
                     /locus_tag="K0W65_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBI3095844.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3829..5610)
                     /locus_tag="K0W65_002634"
     CDS             complement(3829..5610)
                     /locus_tag="K0W65_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBI3095845.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5603..7375)
                     /locus_tag="K0W65_002635"
     CDS             complement(5603..7375)
                     /locus_tag="K0W65_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBI3095846.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7405..7863)
                     /gene="decR"
                     /locus_tag="K0W65_002636"
     CDS             complement(7405..7863)
                     /gene="decR"
                     /locus_tag="K0W65_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HBI3095847.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(8017..8835)
                     /gene="cof"
                     /locus_tag="K0W65_002637"
     CDS             complement(8017..8835)
                     /gene="cof"
                     /locus_tag="K0W65_002637"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBI3095848.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8935..10626
                     /locus_tag="K0W65_002638"
                     /pseudo
     CDS             8935..10626
                     /locus_tag="K0W65_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10691..11386
                     /gene="queC"
                     /locus_tag="K0W65_002639"
     CDS             10691..11386
                     /gene="queC"
                     /locus_tag="K0W65_002639"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBI3095849.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11438..11836)
                     /gene="fadM"
                     /locus_tag="K0W65_002640"
     CDS             complement(11438..11836)
                     /gene="fadM"
                     /locus_tag="K0W65_002640"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HBI3095850.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11930..12301)
                     /locus_tag="K0W65_002641"
     CDS             complement(11930..12301)
                     /locus_tag="K0W65_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBI3095851.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12452..14323)
                     /gene="ppiD"
                     /locus_tag="K0W65_002642"
     CDS             complement(12452..14323)
                     /gene="ppiD"
                     /locus_tag="K0W65_002642"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBI3095852.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14515..14787)
                     /gene="hupB"
                     /locus_tag="K0W65_002643"
     CDS             complement(14515..14787)
                     /gene="hupB"
                     /locus_tag="K0W65_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBI3095853.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14996..17350)
                     /gene="lon"
                     /locus_tag="K0W65_002644"
     CDS             complement(14996..17350)
                     /gene="lon"
                     /locus_tag="K0W65_002644"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBI3095854.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17538..18812)
                     /gene="clpX"
                     /locus_tag="K0W65_002645"
     CDS             complement(17538..18812)
                     /gene="clpX"
                     /locus_tag="K0W65_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBI3095855.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18938..19561)
                     /gene="clpP"
                     /locus_tag="K0W65_002646"
     CDS             complement(18938..19561)
                     /gene="clpP"
                     /locus_tag="K0W65_002646"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBI3095856.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19808..21106)
                     /gene="tig"
                     /locus_tag="K0W65_002647"
     CDS             complement(19808..21106)
                     /gene="tig"
                     /locus_tag="K0W65_002647"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBI3095857.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21450..21767)
                     /gene="bolA"
                     /locus_tag="K0W65_002648"
     CDS             complement(21450..21767)
                     /gene="bolA"
                     /locus_tag="K0W65_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBI3095858.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            22072..22650
                     /locus_tag="K0W65_002649"
     CDS             22072..22650
                     /locus_tag="K0W65_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI3095859.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22694..24169
                     /gene="ampG"
                     /locus_tag="K0W65_002650"
     CDS             22694..24169
                     /gene="ampG"
                     /locus_tag="K0W65_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBI3095860.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5744 a         6731 c         5900 g         6078 t
ORIGIN      
        1 ccatgccaac aatacaccag accatcattt cgaggggaag acggcgcttg cgtagcgtta
       61 cagtacctga ttcggcaagg caacgagaga tgagttcggg ggcgaggtaa tcccccagag
      121 aagtcagtgg gttacgcaga gaatcgtaac gggataccag atcaagagcc tgtccaatgt
      181 gcataaaaaa atccggaaac aagtgagcgt ttccggattc ttacacagcc actggatcgg
      241 tcaactgatc cttaactgat cggcattact ccgggcggag agggttaacg tcgcttaata
      301 ttgcgtagga gcgggaactg ccgaagacgg attcacctga gttggtgaag tcgaaggtac
      361 ggtagtcgtt gcgccaccgc tggcctgaac cggcacggcg gtttgctgta ccggcaccaa
      421 tgtcaggtcg gctttagttc cgccctggtt gatcaccggc tgaacggtat cggtgataaa
      481 taccagtttg tcattcacgg taatcgccgc actcaacaga atacgcgcgt tcggctgaac
      541 atctgccggg ttaaatggca gaacaaagct gaatggtgac tgtttacctt cagtacgcac
      601 cgctttctgc gccagcactt ttgacggtgc atcggctaac gacgcgtcag aaagtgtcac
      661 ggtcagcaca gcatcaggcg gcagtgcgac tttctgacgg atccagacag taccggagac
      721 attcggttgc tggatagcag gttgttgtgt tgctgaaata gacgtatttg cagccggggc
      781 tggcgtctga atatccgcgc ttttatctgc gcaagccgcc aacgcaatcg caaccgctaa
      841 accactggcc atgtgcacga gtttcattca tttctcctta ttatcaatgc accagcgggc
      901 taactttcct cgccggaaga gtggttaaca aaatagtaac gtcaacaagt gtggcacaca
      961 tcacgcattt ctgcctgtaa ttagcccgta attcagacta tttcacccat cggaccagtg
     1021 gcaaatttgc gttatactca actcactttg gcttgctgcg gcagctttgt tactggagag
     1081 ttatatgagt caggcgctaa aaaatttact gacattgtta aatctggaaa aaattgagga
     1141 aggactcttt cgcggccaga gtgaagattt aggtttacgc caggtgtttg gcggccaggt
     1201 cgtgggtcag gccttgtatg ctgcaaaaga gaccgtcccg gaagagcggc tggtacattc
     1261 gtttcacagc tactttcttc gcccaggcga tagtaagaaa ccgattattt atgatgtcga
     1321 aacgctgcgt gacggtaaca gcttcagcgc ccgccgggtt gctgctattc aaaacggcaa
     1381 accgattttt tatatgacgg cctctttcca ggcaccagaa gcgggtttcg aacatcaaaa
     1441 aacaatgccg tccgcgccag cgcctgatgg cctgccttcg gaaacgcaaa tcgcccaatc
     1501 gctggcgcac ctgctgccgc cagtgcttaa agataaattc atctgcgatc gtccgctgga
     1561 agtccgtccg gtggagtttc ataacccact gaaaggtcac gtcgcagaac cacatcgtca
     1621 ggtgtggatc cgcgcaaatg gtagcgtgcc ggatgacctg cgcgttcatc agtatctgct
     1681 cggttacgct tctgatctta acttcctgcc ggtagctcta cagccgcact gcatcggttt
     1741 tctcgaaccg gggattcaga ttgccaccat tgaccattcc atgtggttcc atcgcccgtt
     1801 taatttgaat gaatggctgc tgtatagcgt ggagagcacc tcggcgtcca gcgcacgcgg
     1861 ctttgtgcgc ggtgagtttt atacccaaga cggcgtgctg gttgcctcga ccgttcagga
     1921 aggggtaatg cgtaatcaca attaaaaaaa tagccggagg tgaaaaccgt ccggctattt
     1981 tttgcagtgc ttgttacgcg ttataggcat tctcgccgtg gctgttgacg tccagccctt
     2041 ctcgctcttg ttcttccggc acacgtaggc caaccgtcag atccgccagt ttgtagccaa
     2101 taaatgccac cacaccggac cagacgatcg taatggcgat gctttccaac tgtaccagca
     2161 actgatggcc catcgtcaca ccttcagcga agcccacgcc gcccagagaa ctggcggcaa
     2221 aaatcccggt catgatacag ccgacaatgc cacaaacgcc gtgcacaccg aagacatcgc
     2281 agggatcatc cacccgcagc aagcgtttga gcatggtaac gccccacaaa cccgccagac
     2341 cagctaccac gccgataatc aacgcgccgc caaccccaat gtagccgcac gctggcgtca
     2401 cgccgaccag accggcaatc gcgccagaac acgctcccag cagagaaggc ttaccacgca
     2461 gcgcccattc accgaagatc cagccaagaa ttgccgccgc cgttgcgacc acagtattca
     2521 caaatgccag tgccgcgatt tcattcgccg tgcccactga cccggcgtta aagccaaacc
     2581 aaccgatata gagaatggca gtcccggtga agaccatcgg caggttgtgc ggtttaaacg
     2641 cctctttacc gaagcctacg cgtttgccta tcagatacgc gcccaccaga ccggcgattg
     2701 ctgcgttaat gtgcaccacc gtgccacccg cgaaatccag cgcaccgtga gaagccagca
     2761 aaccaccgcc ccacaccata tgcgcaatcg gaatgtaaga gagcgtcagc cataccacca
     2821 cgaaaatcaa cacagctgag aagcggattc gttccgccag cgccccaact atcaagccta
     2881 cggtaatgca ggcaaacgat ccctgaaacg ctacgtggat atactgataa atgctgccca
     2941 tcaccgccgt cagttcgatg ttttttagca tcaaccagtt aatgttgccg aagaagttgt
     3001 tgccctcacc aaacgccagc gaataaccgt aaaccaccca gagaatacag accagtgcaa
     3061 acgtcaccgt cacctgcgtc agcatcgaca gcacgttttt gccgcgaatc aacccaccgt
     3121 aaaacagggc aatccccgga atagtcataa acagcaccag cgcagtacaa atcatcataa
     3181 acgcattgtc ggctttatcg gccaccgcag gtgcagccat taccagtccc ggcagcatcg
     3241 ccagtgaagc aagcccagtt tttatcgtcg ctatcttcat ttttcgttcc tgttgctgtg
     3301 tgccagagat tacagcgccg cttcgtcggc ttcgccggta cgaatacgaa tgacgcgttg
     3361 caattcagcg acgaagattt tgccgtcgcc aatttttccg gtgtaagccg ccttactgac
     3421 gatatcgatc acttcatcga gttggtcatc agcaatcgcc acatcaattt ttacttttgg
     3481 caggaagttg acgctgtatt ccgccccccg gtacagctcg gcatgccctt tctgacgccc
     3541 gaaacctttc acttcggtga cggtcaggcc ctgaatacca atggaagata acgcttcacg
     3601 aacgtcttcc agcttgaatg gtttgattat cacggtcacc agcttcatag atcccctccg
     3661 gtcagaattc ggtaatggtt ctgctacacg aagaaggtat tgcaagcggt gtgccagaaa
     3721 tgaaaaacga gaaagagagc aggaagttaa gaagattgaa aatgaaaacg cactatgaca
     3781 gtgcacagtg cgttacattt ttgcaccaac gatgaaatat tgcgctattc aggcgctcaa
     3841 tgattcctct tcacgcacgc tggctgccag ctcttcgccc gcaagttgca gttgatacat
     3901 ctgccagtag cgtccctggg ccgccagcag ttgctggtga gtgccctgct ccacggcttg
     3961 cccacgatga agcaccagaa tggtgtcggc atcaataata gttgataagc ggtgagcaat
     4021 caccaccagc gtggtatgtt cacgcaccgc cgccagagca tgttgaatcg cctgttcagt
     4081 accggagtca atgctggcgg ttgcctcatc aaggatcagg atttgcggcg tctccaccag
     4141 cacgcgcgcc agtgccagca gttgcttttg cccgactgag agattatttc cctgctcgcc
     4201 cagcggcgta taaatacaat cactcatgct gcgcgccagc tccgccagtt gcacggtttc
     4261 cagcgcctgc cagacgcgtt cttcggagat atcccgcccc agcgtcacat tagcgaggaa
     4321 ggtatccgcc agcaccaccg gatcttgctg caccattgcc acgccctggc gcagcgcgct
     4381 atgacttagc gaacttaatg gacgaccatc aaggtgaatc tcaccttccg ttagcgggta
     4441 atagcccatc aataaactgg cgagggtact tttaccactg ccggtatgcc cgaccagcgc
     4501 cacaaagtta cgcgaaggca cagagagatt aatgttcttt agcaccagat tgtcatcgcg
     4561 ataagcaaat gacacgttat cgacttcgat ggtgccactc tgtaacgggt aatcatcatt
     4621 gccatattgc tggcgcggtc cgtccatcag ttcaaacacg cgctcaccag caacaacagc
     4681 ctgttgcagc atcgcctgtt gcgtggtcag ttcgattaag ggttcgttaa gtcgcccaag
     4741 atagctgata aacgcataca acacgcccac ttcaatggtg ccgctggcgg agaagccaaa
     4801 cagcatcaat aagccacaaa gaatgagcga tgaaaacaga ctcagcagcg gacgcagcag
     4861 aaaaccgtcg aggcgcaggg tttgcatcct agccatatag tgtgaacgac tggcctcccc
     4921 catacgttcg ccaaatcgcg cctgctgacg aaactgctgg ataacgctca tgccattgat
     4981 gatttcgtta aagccgtcgt tgatatccgc cagataggcg cgcacacggc ggacaatcgg
     5041 cgtgctgtaa cgctggtata tcaccattac caccagcacc accgggaaaa tcattatcgc
     5101 caccagcgcc attcgccagt cgaggctgaa catcgccacc agcatcgcgc ccaccagtgc
     5161 ggcactgcgc aggacagttg ccactacggt aacgtagaga tcgcggatca cttcagtgtc
     5221 attagtgacg cgggaaatca cctgcccgac gggttgggta tcaaactcgc ttaatggctg
     5281 gcgtaacgca gcatccatca cgtcggtacg caactgttgc actgcgccta ctgccgcccg
     5341 attaaacagc agcgactgcg cgtaatgtag cccggcggca aacagttgta gcccaacata
     5401 cgccgcagcc agccctgcaa ccactttcaa cggcaggtta tttttcgcta ccatattgtc
     5461 gataaaatag ctgataagca gcggcccact gacttctgcc gccgccgcaa cccacatcat
     5521 caggaccgca atccccagcg gtttacgcca cggcgaacca tacgctaaca ggcgcttgag
     5581 agtcggccac agttggctaa aactacgcat cgacggcctc ctcgcgaatt tccggaacgt
     5641 cgtcgagcgc cgcctccagt tgttgatagc gatacatatc gcgataccaa ccgctttgtt
     5701 gcgccagctc atcatgattg ccacgctggg cgatatgtcc gtgctgcatc acaataattt
     5761 cactggcttc cgtcagtgcc gaaaggcgat gggcactgat gattaccgtt cttccctgtc
     5821 cccactgacg caggttatgc aggatctggt gctctgtgcg tccgtccacc gccgaaagcg
     5881 catcatcaag gatgaggatt tccgcgttga ctaataacgc tcgagcaatg gagatacgct
     5941 gtttttgccc accggaaagc atcacgccgc gctcgcccac ttctgtatcg taaccttgcg
     6001 gtagacgcaa aatatcgtca tgtacgctgg ctaaccgcgc gacatgctca atctcttgct
     6061 gggtggcatt cgggcaaccc agcgcgatgt tattcgccac ggtgtcagaa aaaaggaatg
     6121 gcgtctggct gaccaccgcc aggcggctac gccagctatc gagttgtaac ttcgtcagag
     6181 gaatatcatg aaagcgaata tccccctcgc tgacgtcgaa atgacgctga atgagcgaca
     6241 acagggtact tttgccggaa ccagtcggcc cgcaaatacc cagcatctga ccgggtttca
     6301 gggcgaaatt gacgttttcc agcgcaggat ggtcagtctg cggatacgtg aactggtgaa
     6361 tatttacatc cagttcgcca cgcccttccg gcacgggttc gctaccatcg ttcaccaccg
     6421 gcgcttccgc cagcatggcg cgaatacggc tgtatgcagc actaccacgt tccacaatgt
     6481 taaacatcca tgccagcgcc agcattggcc aaatcatcag acctaaatac atcataaaac
     6541 tggtgagctg gcccagcgtt aaactgccct gcaccaccat ccaactaccg ccgccaatcg
     6601 ccagcaagtt cgccatacca atcgcgatat agatggtcgg gtcgaagcga gcatcaatac
     6661 gcgccacccg catgtttttt ttgccggtat cttcggcatc cgcggcaaat aacgccgact
     6721 ggcgatcttc cagaccaaag gctttgatca tgcggatact ggtgaggctt tcctgggtgc
     6781 ggtcattaag actggaaaac gccgcctgtg ccagcttaaa gcgttcatgc aaggcatcgc
     6841 cgttgcgctt aatcatgatc gccatcactg gcatcggtaa cagggcaaat aaggtcaact
     6901 gccagctaat ttgcgtagac atcataatca acacagcgca gcccattacc agtgaatcca
     6961 ccagcgtcag caccccttct ccggcggcaa acacgacgcg atcgacgtca ttggtcgcac
     7021 gagccatgag atcacccgtg cgatgacgca ggtaaaactc cgggtgctgc cggcttagct
     7081 gacggtaata atcttcacgc agttcaacag ccagttggta agacgcgcca aacagcaata
     7141 cgcgccagac ataacgcagg agataaacca caacggcaat cagcaccatg gtggcgatcc
     7201 acatcaggct cagcccggta gtaaagtgtt gttctgtcac gccatcgaca acaataccaa
     7261 ccacttttgg cggaaccagt tgcagcatcg cgataatgac aagcaaggcg acagccccaa
     7321 gatagcgacg ccattcccga cggaaatacc agcttaattg agcaaataat cgcacgcgtt
     7381 gtgtcctgac ctgattctgg atatttattc gatgggtaaa gaagtggtgt atttaatctg
     7441 ttccatcgcg aagctggaag tgacgtccga cagccccggc acgctgttta ccagacgctt
     7501 ataaaactcg tcgtagcgtt tcatgtcggc aacctggacg cgcatcagat aatcgtattc
     7561 accagccatg cgccagaccc ccagcacttc tggcatttcg gtaaccaccg tgacaaagcg
     7621 gcaataccat tcgctgctgt gatgttgcgt ttttatcagc acaaaggcgg tcaggccgag
     7681 gcctattttt tccggatcca gcagggcgac tttgccgata aggataccgt cgtcctccag
     7741 ccgtttcagg cgcttccagc agggggtggt tgtcagatta acggcttcag ccagtgcctg
     7801 caaagagagg gtgcaatcct gctgcagtaa ggccagcagc ttacggtcaa ttttatctaa
     7861 catagccctt ccacggagaa tttttttctc ttttaattct attttaaagg tgaaatcgcg
     7921 acaatttatt ctgtgcggtt taaacaccac ggcacaaaat gacaaattca acgcaaaaaa
     7981 aaaccgggca attgcccggt gctggaaggg atctcgttat tcgggggaat aaggtagatg
     8041 tggatagtcc agccagtgcg tcaaatagtg agagacagcc tgatttcggc aatgtccaat
     8101 caccggtaaa tgcgggagct ccgcgcgcag ttgcggcatc gcattgccca taataaaccc
     8161 gctaccgacg ctgcctaaca tttcgcgatc gttcatcgca tcaccaaagg ccatgcaatc
     8221 gcgcaacgat aaacctaaat gttgggtccg caccgtcaat gcagcgcctt tattgcagcc
     8281 caccggcagc acttcgaggc aatccgtggc ggaaaaacac aaatgtgcac gctcgcctaa
     8341 tgcttcgtat agctggatct gcaagcgtgt aagatcgtcg tgatcgccac agaagcagat
     8401 cttggtgacg ctgccgagtg gcattttttt gacatcgatt atctgataac gaaaaccgct
     8461 atagacaaat acctgcaaca acgcagggct ctctttcccg gtaaaccaac cgtcgtcatt
     8521 gaagatatgc atgctggctc gggtatccca ttgctgatac agcaccagct ccgcgacatc
     8581 cgcaggtaaa tcatcacgat gtaaaagttc accttccaga gaatgcacgc gcgttccgtt
     8641 gccggtaatc aaatatgcat ccagcgatag cgccccgaga atatgctgca tctccagcgc
     8701 atgacgcccc gtggcaaaag tgagggtaat gtcgcgttca cgcagtcgcg ccaaagtaga
     8761 gagggttttc tcacctaaat gatggtcagg catcaataaa gtgccatcca tatcaaatgc
     8821 taccagacga gccatttctt tctccactct gtgacacggt taatggtgat tgagtatcac
     8881 ctgatatata cggaagtaat agtgaatagt taaataatat tgttccgggt ttatatgcga
     8941 ttgctcaacc gtcttaacca gtatcaacgt ctgtggcaac cttcagccgc aaaccgtcac
     9001 cgtcagcgaa ctggccgaac gctgtttttg cagcgaacgc catgttcgta cgctgttgcg
     9061 tcaggcacag gaggcgggat ggctggagtg gcaggcgcag tcaggacgcg gaaagcgcgg
     9121 acaattacgc tttctggtca cgccggaatc gctacgcaat gcgatgatgg aacaggcact
     9181 ggaaaccgga aagcagcaag atgtgctgga gctggcgcaa ctggccccag gtgagctgcg
     9241 cactctgtta cagccattta tgggcggaca atggcaaaac gatacaccca cgttgcgtat
     9301 tccctactat cgcccgctcg aaccgctaca accaggcttt ttgcccggcc gtgccgagca
     9361 gcatctcgcc gggcagatat tttccggcct gacccgcttc gataataata ctcagcgccc
     9421 gattggcgat ttagcgcatc actgggaaac ctctactgac aggttacgct gggactttta
     9481 tcttcgttca accctacact ggcataacgg cgatgcagta aaagcctcac acttacacca
     9541 gcgattattg atgctgttac aactgccagc actggatcaa ttatttatta gcgtgaagcg
     9601 tattgaagtc acccatccgc agtgtctgac cttcttttta catcgccccg attactggct
     9661 tgcgcaccgg ctggcgagct attgcagcca tctggcgcat ccgcaattcc cactgatcgg
     9721 cactggtcct tttcgcttaa cacaattcac agcggagctg gtgcgcctgg agagccatga
     9781 ttattaccat ttgcgtcatc cgctgcttaa agcggttgag tactggataa ctccgccgct
     9841 tttcgaaaaa gatttgggaa ccagttgtcg gcatcccgtg caaatcacca tcggcaaacc
     9901 ggaggagctg caacgggtca gccaagtcag tagtggcatc agtttaggtt tttgctattt
     9961 aacgttgcgc aaaagtcccc gactctccct ctggcaggcg cgaaaagtga tctccattat
    10021 tcatcaatcc ggtttattac aaacgttaga agtcggagaa aacctgatca ccgccagtca
    10081 tgcattactg ccaggctgga ctattccgca ctggcaagta ccggatgaag tcaaactacc
    10141 gaaaaccttg acgctggttt atcacctacc gatagaactt cataccatgg cagaacgcct
    10201 acaggcgaca ctggcagcgg aaggctgtga actcacaatt atttttcata acgcaaaaac
    10261 tgggacgaca cgaccctact ggcacacgca gacctcatga tgggcgacag attaattggc
    10321 gaagcaccgg aatatactct ggagcaatgg ctacgttgcg atccactatg gccacatgtt
    10381 ttcgacgctc cagcatatgc acatctgcaa tcgacgctgg acgcggtgca agtaatgcct
    10441 gatgaggaaa accgatttaa tgccctgaaa gcggttttta gccagttaat ggcagacgcg
    10501 acgctgacgc cgctgttcaa ctatcactat cgcattagtg cccctcccgg cgtgaacggt
    10561 gtgcgactga caccgcgcgg ctggtttgaa tttaccgaag cctggcttcc cgcgccgtcg
    10621 caatgaatag ctggtccggg ttaatcgcag gcgttaccat aacgctttta ttgttcaagc
    10681 aggattatct atgaaacgtg ctgtcgttgt gttcagtgga ggccaggatt ccaccacctg
    10741 tctggtgcag gcattacaac aatatgatga agtccattgc gtgacgttcg attacggtca
    10801 gcgacatcgc gcagaaatcg acgtggcacg cgaactggcg ctgaaactgg gagcacgcgc
    10861 gcataaggtt cttgatgtaa cgctgctcaa cgagctggcg gtcagcagcc tgacacgtga
    10921 cagcattccg gtgcctgatt atgaacctga agccgatggc atcccgaata cgtttgtccc
    10981 agggcgtaat attttgttcc tgacgctggc ggcaatatat gcgtatcagg taaaagcaga
    11041 agccgtaatt accggcgtct gtgaaacgga tttctccggc tacccggatt gccgcgatga
    11101 gtttgtgaaa gcactaaacc atgccgtcag tttgggcatg gcgaaagata ttcgttttga
    11161 aacgccgctg atgtggattg ataaagcgga aacctgggcg ctggcagatt attacggcaa
    11221 actggattta gtccgtaacg aaacgttgac ctgctataac ggcattaaag gcgacggttg
    11281 cggtcattgt gcggcatgta atttacgcgc caacggtttg aatcattatc tggccgataa
    11341 accgacggtg atggcagcga tgaagcagaa aaccgggttg aggtaattat tccgggtgtc
    11401 gccggatgcg gcttgagcat ccggcaccac aaaacgttta cttaaccatc tgctccagct
    11461 tttcgcgcaa ttccccttcc agagctaatg ctttctgcgt tttaagatca atacaaacaa
    11521 acgtaataag cgcatccgct accacctgcc cttccggctc cagtgtaatg acctggttta
    11581 agatgccgct tttaccgttt aattgctgca actgactggt aatagttaac aggtcgctta
    11641 ataccgctgg acgacgatag ttaatattga tattgaccac gacgaaggcg atgttatggg
    11701 ccgtcatcca ctgaaaactg tcgctatttt ccaccccatc ccagcgggct tcttcgagaa
    11761 attcaaggta gcgggcgttg ttgacgtgct ggtaaacgtc gagatgatat ccacgaactt
    11821 tgatttgtgt ttgcatagcg caataacctt acgttgttgt tataagtaca gaggtcataa
    11881 ctggtatgac ctcttcagtc tggcaaaaaa ttgccactat gcaaattaat tacagggtta
    11941 ataccgccag attacgttcc accagcgaat tgcccatccc cggcacctgc tttagatcct
    12001 ccaccgtttt aaacggaccg tactcttcgc gataactgac aatcgcctgc gctttcttca
    12061 ggccaacgcc attcatcgcg cgggctagct cttccgcgct ggcattatta atgctgaccc
    12121 gggtgccttc ttcgtcactg gctttcgccg gtactgctgc tttactttgt gctgcaggag
    12181 cttccgcttt ggtttctacc gccgtcggtt tcgccacaga agctgccgcc agcgcgctat
    12241 gagacattcc ggcacaggcc agggacagtg taatgagcag tgctttaatt ccgtgtttca
    12301 tactgttttc tccttgtttg ttaacagtgg aatcacctta acgggagtga aaacagtaaa
    12361 caaatggcaa tgttcagaaa tggaaaaggc cgcgaaagcg accttttctt gttacagctg
    12421 caacgcattt ttttgcgagg cggcttcagg attattgctg ttccagcgca tcgccaattt
    12481 tgattttcgc ctctttacgc aggttactca tcagagcttc aaagacgatt tgtgcgttgt
    12541 tctgggtgat accctgcacc atcgcttttt tctgatcttc cggcattgaa ccttgtttca
    12601 cttcatccag cgccagcaga accacattac cttgcatatc ggtcgccata ccgtagctcg
    12661 gtttgtcttt cgctggcagt ggcagtgcaa acgccgcctg gctaatcggg tcacgaccgg
    12721 aacggcttaa ggttttcggc tcgccaaatt tcagaccggc agcctgcata gcttccgcac
    12781 ctttgccggc tttcaaatca accagcagtt tctcagcatc cactttcgcc tgttgttcag
    12841 ctttgttgtg ctgaaccagt gccttaactt gttcctgaac atctgccaac ggtttcaccg
    12901 cttccggttt gtgctcgctg acgcgcagca cgaatgcgcg gtcgccgtct acggtgatga
    12961 tgtcagagtt gatgcccgac gcgccgtttt cacctaccag accgccgtta aagatagcgt
    13021 cggcaaccgg cttgaagttc aactcttccg gcaggttatc tttgctgaac caacccgtct
    13081 gagtggcttt aacgccggca gcttgctctg caccggccag agactcggtg tcgttgcttg
    13141 ccgcatcgct cactttctgc tgcagcgcat agtacgcatc gagggctttt tcgtgtttca
    13201 ctttcgccgc aatgtcgtca cgtacttcgt ctaacgattt cactttcgct ggctgaatgt
    13261 cgtccagacg tacaatcagg aaaccgaccg aagatttgat gacaccagac agttggcctt
    13321 tttctttcag accagcattt ttcagttcgt cggggatagt ggcatcttct aaccaaccca
    13381 tatcgccgcc gttacgggca gagataatat cggcagattt ttctttggct aatgcagcaa
    13441 aatcaccgcc tttattcagc tcatcaagta ccgctttcgc ttcatcttca gttttggtct
    13501 ggatgatgct gtagcgggta cgctgcggct gggtgaattg atcctgatgc tgatcgtagt
    13561 agctctggat atccgcatcg ctaaccggtt gctgcatcgt tgcggcaacc agcttgatgt
    13621 aactcacgcg gaattgttcc ggcgtcatga aattgttttt gttttgttcg tagtagctgg
    13681 caatctcctg ttcggtcaca ggctgcttcg ccgccagcgc gttaacatcg atagtcgcct
    13741 cacgcaccac gcgttgttgc gcgaccagtg ccgccagctc gtcggtttca cctttcagca
    13801 taaaatcggt accggcaacg ccgttaatca gctgttgggt agtgagctgg ttacgcagcg
    13861 cctgggcgta ctgatcggcg gtcatcccca tctggttgag gataccgtta tagcggctgt
    13921 tatcaaattt gccgtcaacc tggaaggctg gggtcgcgaa aatcgcctgt ttaacctgct
    13981 catcactgat acccagtttc agctcacgag cgtactgatc caacagcgcc tcgtcgatca
    14041 gacgattcag cacctgttga cgcagggttt tcatatagcc ttcgttcgct gccagctcgg
    14101 agtattgatc gcccagctgt tgctgcatac gattacgctc gctgttaaag gcgttctcga
    14161 actgcccacg gctgatttcc tggtcattca cttttgcggc gtagttattg cctccgccaa
    14221 tcaggtaacc actcacgccg gtcaatatga acgacacgat aatgataccg aaaataatct
    14281 tgagcacgag actgtttgca gccgtgcgta agctgtccat catggtgtaa caacactccg
    14341 ctgtagatga ctgtgtacct cacgcaacgt atcgatgcgc gcaaaggcct attgtgacaa
    14401 gaaacggggg caattgtcag cccacaagac acagaaagtc accgaataca aataaaaaag
    14461 gcacatcagt agatgcgccc ttgaacttcg tcacatcccc actggggaca acgcttagtt
    14521 taccgcgtct ttcagtgctt tacctgcacg gaagctcggt actttagcgg cagcgatggt
    14581 gatctcttta ccggtctgcg ggttgcggcc agtacgggca gcacgctctt taacggcaaa
    14641 agtaccaaaa cctaccagtg ctacatcatc cccttctttc agagattcag ttacggaagc
    14701 aataatagca tctaacgcac ggccagccgc agctttagag atatcagccc ctgcagcaat
    14761 cttgtcgatc aattgagatt tattcactct tctcttcctc tttataattt atatcgcact
    14821 tgaatccttc aaggtacgaa cgcgcagcag ttatatcagg ccagccatcc ccttacaaca
    14881 ccccttaata agggcaagcc cgatccgcta tctaacttag cgagacaaaa aaaggctggc
    14941 aagcccgaat tagcctgcca gccctgtttt tattagtgca ttttgcgcga ggtcactatt
    15001 ttgcagtcac aacctgcatg ccagacggtt cattttgcag cgccagagtc agaacttcct
    15061 caatgcgctt cacaggatga atgtccagat cggcaattac gttgtcagga atctcttcca
    15121 gatcgcgttt attttcgaac ggaattagca ctgttttaat cccgccgcga tgcgctgcca
    15181 ggagtttttc tttcaaacca ccgatcggca gtacctgacc acgcagagtg atctcaccgg
    15241 tcattgccac atcggcacga accgggttac cggtcaggca agaaaccagc gcggtgcaca
    15301 tagcaatacc ggcactcgga ccatctttcg gcgtcgcacc ttccggtacg tggacgtgga
    15361 tgtcacgttt ttcgtaaaaa tcagggttga tccccagttt ttccgcacgc gcacgaacca
    15421 ccgttaacgc cgcctgaata gactcctgca tcacttcgcc gagcgaaccg gtataggtca
    15481 gtttgccttt acccggaaca catgcggttt caatggtcag caagtcaccg cccacttccg
    15541 tccacgccag accgattacc tgaccgacac ggttttcgtt atcagcgcga ccatagtcga
    15601 aacgctgaac accgaggtag tcatgcaggt tatcgccgtt aatttcgata tgttttaatg
    15661 acttatcgag cagtaactgc ttaaccgctt tgcggcacag tttggagatt tcacgctcca
    15721 gaccacgcac gcccgcctca cgggtgtagt aacgaataat gccgataatg gcgctatcgt
    15781 cgacggtcag ctcacctttt ttcagtgcat tacgttcaat ctgcttcggc agcaggtgac
    15841 gtttggcgat gttcagtttt tcatcttcgg tataaccgga gaggcgaatc acttccatac
    15901 gatccagcag cggtgccgga atgttcatgg agttcgacgt cgcgacaaac atcacgtcgc
    15961 tgagatcgta atccacttcc aggtagtggt cgctgaacgc tacgttctgc tctggatcca
    16021 gcacttcaag cagtgcagag gccggatcgc ctcgcatgtc agaagacatt ttgtcgatct
    16081 catcgagcag gaacagcgga tttttcacgc ccactttcgc cattttctgg atcagtttac
    16141 ccggcataga accgatgtaa gtacggcggt gaccacggat ttccgcttca tcacgcacgc
    16201 cgcccagcgc catacggaca tatttacgcc cggtggcttt ggcaatggac tgaccaagag
    16261 aggttttacc tacccccggc ggccctacca ggcagaggat cggtcccttg attttgttga
    16321 cacggctttg aaccgcaagg tactcaagga tgcgatcttt cacgcgctcc agaccataat
    16381 ggtcggtatc aaggatttcc tgcgcctgac gcaggtcttt tttgaccttg ctgcgcgcat
    16441 tccacggtac ctgtaccatc cagtcgatat aaccacgcac tacggtcgct tctgccgaca
    16501 tcggagacat cattttcagc ttctgcaact ctgcttccgc tttctctttt gcctctttcg
    16561 gcatcttcgc cgcgtcgatt ttgcgcttca gggcttcgtt ttcgtccggc acgtcgtcca
    16621 tttcaccgag ttctttctga atagctttca tttgctcgtt cagatagtac tcacgctggg
    16681 atttctccat ctgcttttta acgcggttgc gaatgcgttt ctcaacctgc agcagatcga
    16741 tttccgattc catcattgcc atcagatatt ccagacgttc gttaacgtcg gacatctcca
    16801 gaacggactg tttgtcagcc agtttcagcg gcatatgtgc agcaatggta tccgccagac
    16861 gcgccggatc gtcgatgcta ttcagcgacg tcagcacttc tggtgggatt tttttgttca
    16921 gcttgatgta gccttcgaac tggctgattg cagtacgcac cagcacttcc tgttcccgct
    16981 catcaatggt cggcgactcc agatactccg ccttcgcaga aaagtgttcg ccattgtcag
    17041 agagcgcaga aatacgcgcg cgctgtaacc cctcgaccag cactttgacg gtgccgtcag
    17101 gcagtttcag catctgcaat atagaggcca cggtcccgac ggtgaaaaga tcgtttacac
    17161 ccggctcatc cgttgaagct tctttctgcg cgaccagcat aattttttta tcatggtcca
    17221 tcgccgcttc cagacaacgg atagattttt cccgcccgac aaataagggg atgaccatgt
    17281 gcggataaac caccacatcg cgcagcggca atacggggat ttcaatgcgt tcagaacgct
    17341 caggattcat agagctctct cttagtttaa tttccgccag gtaatcagat gacacgactg
    17401 tgcttcacgc catctattaa catgtacgtc agtatatggg gatgtttccc ccacattcaa
    17461 cgccgagaat agaggaaaaa ttaaagggga gataaaatcc cccctttttg gttaactaat
    17521 tgtatgggaa tggttaatta ttcaccagat gcctgttgcg cttccggctt accataaatc
    17581 agcaacggtt tgctttgacc atcaattacc gactcgtcga taaccacttt ttcgacgtct
    17641 tccatggacg gcagatcgta catggtatcg agcagtgcgg cttctacgat ggaacgcagg
    17701 ccacgggcac cggttttacg cgccatcgct ttcttagcga tagcatccag cgcctcgtca
    17761 cggaattcca gatccacgcc ttccagatta aacagcgcct gatactgctt ggtcagggcg
    17821 tttttcggct ctttgaggat ctgaatcaga gcttcttcgc tcagttcatt caacgttgct
    17881 acaaccggca gacgaccaat gaactcaggg ataagaccaa acttgatcag atcttccggt
    17941 tcaacctgcg ccagcagctc gccttcgctt gctttgtcgg acttcgcttt taccgtcgcg
    18001 ccaaaaccaa tgccggagcc ggtttctaca cggtgggaaa tcactttatc cagaccggca
    18061 aacgcaccgc cacaaataaa caggatctta gaggtatcaa cctgcaagaa ttcctgctgc
    18121 ggatgtttac gcccaccttg cggtggaaca gcggctaccg taccttcgat cagtttcaac
    18181 agtgcctgct gtactccttc accggaaacg tctcgggtaa tggacgggtt gtctgactta
    18241 cgagaaatct tgtcgatttc atcgatgtag acaatcccgc gctgcgcttt ctgtacgtcg
    18301 taatcgcact tttgcaacag cttctgaatg atgttttcaa cgtcttcgcc cacataaccg
    18361 gcttcggtca gcgtggttgc gtcggccatg gtgaacggga cgtccagcag gcgcgccagc
    18421 gtttcagcca gcagcgtttt accggaaccg gtcggaccga tcagcagaat gttacttttg
    18481 cccaactcga caccattgct ggtatcgccg ttgcgcagac gtttgtaatg gttgtatacc
    18541 gcgaccgcca gcactttttt cgcctgttcc tggccgataa cgtaatcgtc caggtggttg
    18601 cgaatttcat gcggcgtcgg tagcgcactg cgttcacgat gcggtgcaac ttctttaatc
    18661 tcttcgcgaa tgatgtcgtt acataaatca acacattcgt cgcagatata cacggatgga
    18721 ccggcaatca gcttgcgcac ttcatgctgg cttttgccgc aaaaagagca atacagcaat
    18781 ttgcctgagc catctttgcg tttatctgtc atgggtcaaa acctcttctt tgttctttgt
    18841 gccgcacacg acgacgcaaa tgccattctc aggcgcaagc cgcttacagc gttgtgccgc
    18901 cctagataag tatagcggca cagttgcgcc tctggcatca attacgatgg gtcagaatcg
    18961 aatcgaccag accgtattcc accgcttcag gggcggaaag gaagcgatcg cgctcggtat
    19021 cacgttcaat ctgttctaat gattgacccg tatgaagcgc cataagttca ttcatgcgcc
    19081 ctttaacttt cagaatttca cgggcatgaa tttcgatatc ggtcgcctgg ccctggtagc
    19141 cgcccaacgg ttggtgaatc atcacgcgcg aattcggcag gcaaaaacgt ttaccttttg
    19201 cccctgcggt caacaagaaa gcgcccatcg aggccgcctg gcccatacag atggtgctga
    19261 catcaggctt gataaactgc atggtgtcat agatagacat cccggcagtg atcaccccgc
    19321 ctggggagtt aatgtacaga tagatatctt tttctgggtt ttccgcttcc aggaacagca
    19381 tctgcgccac aatcaggtta gccatgtggt cttcaacctg gccagtcaga aaaatgacgc
    19441 gttccttaag tagacgagaa tagatatcaa aagagcgctc accgcgtgag gtctgttcaa
    19501 tgaccatcgg caccagcgcc atatggggtg caaagttatc tcgttcgccg ctgtatgaca
    19561 tttccgtctc ctggataaaa ttgaaaaaaa cctgctgtac cgattgtaac cttatggacg
    19621 gattatcagc tagtccctgt gttatgggta cggcatcacc atatttcaag cataacaatc
    19681 ttttgttgtt acgctaacac cgaaagaggc ttttcgcacg gctccatgca aaattcatga
    19741 caaaaaaaag cccaccacct ggcggtgacg ggcctttgtg cgaatttagc gcgttatgct
    19801 gcgtaaatta cgcctgctgg ttcatcagct cgttgaaagt ggtttctttt tcagtcactt
    19861 tcgctttcgc cagtacagct tcaacagcct gttcttccag agcaacattg cgcatgttgt
    19921 ccatcagttc tttgtttttg ctgtagaact cgataacttc tttcggatct tcgtacgcag
    19981 aagccatctc ttcgatcagg cctttcacgc gctcttcgtc agctttcagc tcgttggtgc
    20041 ggataacttc gcccagcagc aggccaacaa ctacgcggcg tttagcctgt tcttcgaaca
    20101 gttcgcgcgg cagttccaga gcttgttttt cgttgccacc gaaacgctgt gcagcctggc
    20161 gacgcagaac gtcgatttcg ctgtcgatca gcgcagccgg tacgtcgatg tcgttagctt
    20221 ttaccagacc ttcgatcgcc tgagacttaa cgcggttacg gatggcgctc ttcagctcgc
    20281 gctccatgtt tttacgcact tcagcgcgca gaccttctac ggaaccatct tcaacgccga
    20341 aacgtttgat gaattctgca gtcagttccg gcagttcacg ctcttcaact ttcttcaggt
    20401 tgatagcgaa tttcgctgct ttacctttca ggttttctgc gtggtattct tccgggaagg
    20461 tcacgtcgat ggtgaactct tcgccagctt tgtggccttt gataccgtct tcaaagcccg
    20521 ggatcatacg accctggccc atcgccagta cgaaatcaga cgctttaccg ccttcgaact
    20581 cttcgccgtc tacagaaccg gtgaagtcga tggttacgcg gtcttctgct tcaacagcgc
    20641 cgtctttttc tttccaggtc gcctgctgtt tacgcagagt atccagcatg ccgtcaacgt
    20701 cagcgtcggt cacttcaacg atcggttttt caacttcgat cgcttccaga ccctgcagtt
    20761 caacttccgg ataaacttca aactctacag agtaagtgaa gtcttcaccc agcttgtatt
    20821 cgcccggaac ataagtcggt gcgccagccg gattgatttt ttctttaatg atggcgtcaa
    20881 tgaagttacg gctcatcagg tcacccagaa cgtcctggcg tacagacgcg ccataacgct
    20941 gagcaacgat attcattggc actttgcctt tgcggaagcc gtcaatacgt acttttttcg
    21001 caacgttgac cagctcgctt ttaacagcgg tctcgatgct gtcagcagcg atagtaatcg
    21061 ttacacggcg gccaaggcct tgagtggttt caactgaaac ttgcatcttg ttacctcaaa
    21121 aaatcacagt gctcggtcaa ctcttacccc gcaagcacag gttgttggct tcgcggaacc
    21181 gggatgttct tataatcaat cacattccct gttaccagaa tcatcccgaa agcattcaaa
    21241 taggacgcgg cattatagcg gcatcacttt tatgagtcga gaacggttat tgcgcgctgt
    21301 cgcgtatttt ttcacaattc ccgggcaatt ttcgtctgaa tgctgggcaa aaagcgcaaa
    21361 atccagacaa aacaaaacgg cccgcaggcc gtttttcata aaatctttag caacatactg
    21421 gaaaagcgcc gacagttgca aatgcgtttt tacgcgatgc ttcctgctcc acgacaggga
    21481 ggagaggcaa agacggtgtc ctgcaaccct tcccactcct taatagtgta agtatgcaga
    21541 gccagcgcat gaacggtagt agagagttcc tccgctaaag tactgtaaat cattcgatga
    21601 cgattcagaa aacgttcacc cgtaaaacga tcgctgacca gcacaacttt aaaatggctt
    21661 tcagagccgg ctgggacatt gtgacgatag ctttcatcca ctacttcgag gaatacgggt
    21721 tggaacgccg cccttaattt ttcttctatc cgctcacgta tcatcatgaa atatctcctc
    21781 cgacaacgct gagatgtcat ccatcccttt aaatactagc cgcttttacc gtttccattg
    21841 cagcttaaca acaaatattt aggtttcgtc ctggtttttc cgtcaagcgt atgaagttta
    21901 ctgatttaaa gattgaaaca caggcctcgc atcagcgttt cttttaccag gggtgattat
    21961 ttcgtcaaca tgatgaattt acatgcctta cccacttccc ctcgccgttg gcaatgttat
    22021 gatggcggga atttttacca aacacgctta agagtctctg agagcccgaa catgttcaaa
    22081 aaaatcctct tcccgttagt tgctctgttt atgcttgcag gatgcgcaaa accgccaaca
    22141 actattgaag tttccccgac gattacactg ccacagcagg atccaagcct gatgggcgtc
    22201 accgtaagca ttaatggtgc cgatcagcgt accgatcagg cgctggcaaa agtcacccgc
    22261 gataatcaaa tcgttaccct gaccgcctcc cgcgctctgc gtttcctgct gcaagaagtg
    22321 ctggaaaaac agatgaccgc gcgtggttac atggttggtc cgaatggccc ggttaatctg
    22381 caaatcatcg ttagccaact gtatgctgac gtgtcccagg gcaacgtgcg ctacaacatc
    22441 gctaccaaag cggatatcgc catcatcgct accgcgcaga atggcaacaa aatgaccaaa
    22501 aactatcgtg ccagctacaa cgttgaaggt gcgttccagg cctccaacaa aaatatcgcc
    22561 gatgcggtta acagtgtgct gaccgatacc atcgctgata tgtctcagga caccagcatt
    22621 cacgaattca tcaagcagaa cgcgcgttaa tttctgccct ctggcccggt gcaagccggg
    22681 cctgtagacg cccatgtcca gtcaatattt acgtattttt caacagccgc gttcagccat
    22741 attgctgatc ctgggttttg cttccgggct accgctcgcc ctgacatccg gcaccttaca
    22801 ggcctggatg acggtcgaaa atatcgatct caaaaccatt ggtttcttct ctctggtagg
    22861 ccaggcttac gtttttaaat tcctctggtc accgctgatg gaccgctaca cgcccccatt
    22921 ttttgggcgg cggcgcggtt ggctgctcgc cacgcaaatc ctgttattag tcgccattgc
    22981 ggcgatgggt tttctcgaac caggcaccca actccgctgg atggcggcgc tggcggtggt
    23041 gatcgctttt tgctctgcct cccaggatat tgtcttcgat gcgtggaaaa ccgatgtgct
    23101 cccggcagaa gaacgtggtg cgggcgcggc aatcagcgtg ctgggttacc gtttagggat
    23161 gctggtttcc ggcggcctag ccctgtggct ggcagataaa tggttgggct ggcagggcat
    23221 gtactggttg atggcggcgc tgttgatccc ctgtattatc gcgacgttgc ttgcaccaga
    23281 accaaccgac accattcctg tgcccaaaac gctggaacaa gcggttgttg cacctctgcg
    23341 agatttcttt ggtcgcaata atgcctggct tattttgctt cttatcgtgc tgtataagct
    23401 gggcgacgca ttcgccatga gcctgacaac cacgtttttg attcgcggcg tcgggtttga
    23461 tgcgggtgaa gtaggcgtgg taaacaaaac gcttggctta ctggcgacca ttgttggcgc
    23521 attgtacggt gggattttga tgcagcgcct gtcgctgttc cgggcgctgc tgattttcgg
    23581 cattttacaa ggtgcgtcta acgctggtta ctggctgctg tcgattactg ataagcatct
    23641 ctacagcatg ggcgcagccg tctttttcga aaacctctgt ggcgggatgg gcacatcagc
    23701 ctttgtcgcg ctgttaatga cgctgtgtaa taagtcattt tccgctactc aatttgccct
    23761 gctctcagcg ctttccgctg tagggcgagt ttatgtcggc cccgtggcgg gttggtttgt
    23821 tgaagcacac ggctggtcga cattctatct attctccgtc gccgctgccg taccggggct
    23881 tattttgctg ctggtttgcc gccagacgct tgaatataca cgagtaaatg acaactttat
    23941 ctcccgtacc gaatatccgg caggttatgc ctttgccatg tggacactgg cggcgggcgt
    24001 cagcctgttg gccgtgtggt tacttctgtt gacgatggac gcgctggatt tgacgcactt
    24061 ctctttcctg cctgctctgc tggaagtcgg ggttttagtc gccctttctg gcgtcgtgct
    24121 tggtggtttg ctggattatc tggcgctacg aaaaacgcat ctgacgtaat ctgtaaatat
    24181 tatttagctg tagtaatcac tcgccgtaat tattacggct aaataaacat cagcatcgct
    24241 tattaatatt tgttcttttg tgcggcttag cgtttggaaa ttattgacgc catttataaa
    24301 ttctattttt cttacacgat tcagctaatg agtctttatt ttctcaccac ccagttgtca
    24361 ctttaatgat aattatttgt taaataattg ttttatttcg cattggttat accaattgcc
    24421 caccaagact ccttctgcac tccccttttg tta
//
LOCUS       DAETQL010000061        24124 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.061, whole genome shotgun sequence.
ACCESSION   DAETQL010000061 DAETQL010000000
VERSION     DAETQL010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.061"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..180
                     /gene="yjbD"
                     /locus_tag="K0W65_002651"
     CDS             <1..180
                     /gene="yjbD"
                     /locus_tag="K0W65_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBI3095861.1"
                     /translation="RVLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPE
                     ASFSWSANTSTRGRR"
     gene            complement(313..1185)
                     /gene="rluF"
                     /locus_tag="K0W65_002652"
     CDS             complement(313..1185)
                     /gene="rluF"
                     /locus_tag="K0W65_002652"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBI3095862.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1533..2480
                     /locus_tag="K0W65_002653"
     CDS             1533..2480
                     /locus_tag="K0W65_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBI3095863.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2550..3356
                     /locus_tag="K0W65_002654"
     CDS             2550..3356
                     /locus_tag="K0W65_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI3095864.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3366..3773
                     /locus_tag="K0W65_002655"
     CDS             3366..3773
                     /locus_tag="K0W65_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HBI3095865.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3773..4267
                     /locus_tag="K0W65_002656"
     CDS             3773..4267
                     /locus_tag="K0W65_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HBI3095866.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4333..5130
                     /locus_tag="K0W65_002657"
     CDS             4333..5130
                     /locus_tag="K0W65_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBI3095867.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5141..5929
                     /locus_tag="K0W65_002658"
     CDS             5141..5929
                     /locus_tag="K0W65_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBI3095868.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5981..7210
                     /gene="sorE"
                     /locus_tag="K0W65_002659"
     CDS             5981..7210
                     /gene="sorE"
                     /locus_tag="K0W65_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HBI3095869.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7592..8281
                     /gene="pepE"
                     /locus_tag="K0W65_002660"
     CDS             7592..8281
                     /gene="pepE"
                     /locus_tag="K0W65_002660"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HBI3095870.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8372..10003)
                     /locus_tag="K0W65_002661"
     CDS             complement(8372..10003)
                     /locus_tag="K0W65_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBI3095871.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10223..13906)
                     /gene="metH"
                     /locus_tag="K0W65_002662"
     CDS             complement(10223..13906)
                     /gene="metH"
                     /locus_tag="K0W65_002662"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBI3095872.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14106..14930
                     /gene="iclR"
                     /locus_tag="K0W65_002663"
     CDS             14106..14930
                     /gene="iclR"
                     /locus_tag="K0W65_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBI3095873.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15247..17431
                     /gene="espL4"
                     /locus_tag="K0W65_002664"
                     /pseudo
     CDS             15247..17431
                     /gene="espL4"
                     /locus_tag="K0W65_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17400..19137)
                     /gene="aceK"
                     /locus_tag="K0W65_002665"
                     /pseudo
     CDS             complement(17400..19137)
                     /gene="aceK"
                     /locus_tag="K0W65_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19321..20625)
                     /gene="aceA"
                     /locus_tag="K0W65_002666"
     CDS             complement(19321..20625)
                     /gene="aceA"
                     /locus_tag="K0W65_002666"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HBI3095874.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20655..22256)
                     /gene="aceB"
                     /locus_tag="K0W65_002667"
                     /pseudo
     CDS             complement(20655..22256)
                     /gene="aceB"
                     /locus_tag="K0W65_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22525..23454)
                     /gene="metA"
                     /locus_tag="K0W65_002668"
     CDS             complement(22525..23454)
                     /gene="metA"
                     /locus_tag="K0W65_002668"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBI3095875.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23611..24054
                     /locus_tag="K0W65_002669"
     CDS             23611..24054
                     /locus_tag="K0W65_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBI3095876.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5949 a         6161 c         6115 g         5899 t
ORIGIN      
        1 cgcgtactga ccaactccga aaccaacagc attaagaaag agtacatcga taaattaatg
       61 gtcgagcgtg aggctgaagc gaaaaaagcc cgccaattga agaaaaagca ggcttataaa
      121 ccggatcctg aagcgtcatt tagctggtca gcaaatacgt cgacgcgcgg caggcgctaa
      181 tgaacaatca acgcctgaga ttttcactcg tctgtgtgga cacaatcttc ccctcaccct
      241 aaccctctcc ccgaaggggc gaggggactg tccgagctct ttttggacgt tttcatcagt
      301 ctgaggcgtt aatcagcgtc ccttcttttt acgccccggc gaggtaaaac gcttaccgtt
      361 ggacgccggg cgaccgcctt tttccgccgt tttttccatc ttaacgactg gacgtttgat
      421 gcccgctgtt ttcggtttgg ccttcgcttt aggttttgcc tcggaagagg aattttcgat
      481 gagcttaaag aggtcgatta actcatcgtc ggttaaatcg cgccactccc ccagcggaat
      541 gccgcttaag ctgacgttca taatgcgcgt acgttccagc tttttcactt catagccgaa
      601 atgctcgcac atgcgccgga tctgacggtt cagcccctgc actagggtaa tgcggaagac
      661 aaacggcgct tcttttttga ctttgcactt tttcgtcacc gtcccgagga tcggcacccc
      721 cgcccccata ccacgaataa attcatcggt aatcggttta tcgaccgtca ccagatactc
      781 tttctcatga tcgttacccg cacggaggat cttattcacc agatcgccgt gattggtgag
      841 gaaaatcagc ccctgggagt ctttatccag gcggccaatc gggaacacgc gtttgctgtg
      901 gttaacgaaa tcgacaatgt tatcgcgctc gccatcttcg gtggtgctga caataccgac
      961 gggcttgttc agggcgataa gtaccaaatc ttcggcttcc cgaggctcaa tcaactgacc
     1021 gtttactttc acaatgtcgc cgggtttcac ctgatcgcca atggtggctc gcttgccatt
     1081 aaggaacaca ttgccttgct cgatatagcg atccgcttcg cggcgtgagc aaattccgct
     1141 ttcgctgatg tatttattta aacggactga tgagtcgggc agcataaatt ctcctgtaaa
     1201 acgcggaata taccgtacct cttagcgggg aaaaacaggg ttatgcggca acgtcgaaat
     1261 taaatgtgat ctgccacgcc ttttatcaca aaagtgtctc cggaagtacg atgcaaattt
     1321 gcctggcaaa ataaggataa tcaacatatt acgaatcgct gcacacctgt gctgatgact
     1381 actacgcaga gaaacaaatg tgcaagagaa agcgctattt ctccccatgc aaagagtgaa
     1441 aaatcgcttt gaaaggccct catgatgggg ttatatcaga tcaagcggtg agaaagcgta
     1501 tttgcacaaa tgtgcagaac caggaggcgg caatggaaaa cagtgacgat atccgtttga
     1561 ttgtgaagat tgcccaactc tattacgaac aggatatgac gcaggcgcaa atcgcgcgcg
     1621 aactgggtat ttaccgcacc accatcagcc gcttgcttaa acgaggccgc gatcagggaa
     1681 tggtcaccat cgccatcaac tatgactaca acgaaaatct ctggctggag cagcaactga
     1741 agcaaaagtt tggcctgaaa gacgttgtgg tggtgtcggg aaatgatgag gatgaagaga
     1801 ctcaactggc gatgatgggg ttacacggcg cgcaactgct ggatcgcttg ctggagcctg
     1861 gcgatattgt cggtttttcc tggggtcgcg cggtgagcgc actggttgaa aacttgccgc
     1921 aggcggggca atcgcggcag ttaatctgcg taccaattat tggcggaccg tccggtaaac
     1981 tcgaaagccg ctatcatgta aacacattaa cctacagcgc ggcagcgaag ctgaaagggg
     2041 aatcgcatct cgcggatttt ccggctctgc tggataaccc attaattcgt aatgggatca
     2101 tgcagtctca gcactttaaa accatctctg cctactggga taatctggat atcgccctgg
     2161 tgggaattgg ctcaccggcc attcgcgacg gcgctaactg gcatgcgttt tatggtggtg
     2221 aagagagtga cgacctgaat gcccgccagg ttgctggcga tatttgctcg cgcttttttg
     2281 atattcacgg cgcaatggtt gaaacgaata tgagcgaaaa aacgctctct atcgaaatga
     2341 ataaattaaa gcaggcgcga tattccattg gcattgccat gagtgaagaa aaatacagcg
     2401 gaattattgg tgcactgcgt ggaaaatata ttaattgtct ggtaacgaat agcagcacag
     2461 ctgaactatt actgaaataa aaatcttccc gatgtttacc gtaataaaaa ttacggcggg
     2521 tccctattat ctaaaatcag gagtctgtta tgcaaacgtg gttaaatttg caggataaaa
     2581 tcattattgt caccggcggc gcatccggta ttggtctggc gattgtagag gaattattag
     2641 cacaaggcgc gaatgtacag atggtcgata ttcacggtgg cgatggtcaa tatgaaggtc
     2701 ataaaggcta tcagttttgg ccgaccgata tttctagcac aaaagaggta aatcatacgg
     2761 tagcggaaat tatccagcgt tttggtcgca tcgacggtct ggtcaataac gccggggtca
     2821 atttcccgcg tctgctggtc gatgagaaag cgcctgccgg gcagtatgaa ctcaacgaag
     2881 ctgcattcga aaaaatggtc aatatcaacc agaaaggtgt ttttctgatg tcgcaggcgg
     2941 tggcgcgaca gatggtcaaa caacatgatg gcgtgattgt gaatgtttcc tcagaaagtg
     3001 ggctggaagg ctcagaagga caaagctgtt acgccgctac caaagccgcg ctcaatagct
     3061 tcacgcgctc ctggtcgaaa gagctgggta aacacggtat ccgtgtggtc ggtatcgcgc
     3121 ccgggattct ggaaaaaacc gggctacgca cgccggaata tgaagaggcg ctggcgtgga
     3181 cgcgcaatat caccgtcgag cagctgcgtg aaggctatac caaaaacgcc attcctattg
     3241 ggcgcgccgg aagattagca gaagtagctg attttgtttg ttatctgttg tctgaacgcg
     3301 ccagctacat caccggagta accactaaca ttgcgggcgg caaaacgcgc ggctaaggag
     3361 gctttatggt caacgccatt ttttgtgccc acggcaaatt agcttgcgcc atgctggaat
     3421 cggttcagat ggtttacggc aacgccaatg ttgaagccgt tgcatttgtg cccggtgaaa
     3481 acgccggaga cattgcggta aagctggaaa agttagtaag cattcacaat caagatgagt
     3541 ggctgattgc cgtggattta cagtgcggca gcccgtggaa tgcggcagca atgcttgcga
     3601 tgcgcaatcc tcgtttgcgg gtaatcagcg gcctttctct gccgctggcg ctcgaactgg
     3661 ttgataacca ggacagcatg aatgttgatg aactgtgtga acacctgacg caaatcgcaa
     3721 agcaaacctg cgttgtctgg aaacaactgg caacgacaga ggaggatttc tgatgaacat
     3781 tacgctcgcc cgcattgatg accgtctgat ccacggccag gtcactaccg tctggtcgaa
     3841 agtggctaac gcccagcgca ttattatctg caatgacgaa gtttataacg atgaagtgcg
     3901 ccggacatta ttgcgtcagg ctgcgccacc gggaatgaag gttaacgtgg tcaatattga
     3961 aaaagccgtc gcggtttatc acaacccgca atatcaggac gaaaccgttt tttatctttt
     4021 cactcgccca caggatgcct tagcgatggt tcgccagggg gtaaaaatag gcaccctgaa
     4081 tattggcggc atggcctggc ggcccggtaa aaaacagtta accaaagcgg tttcattaga
     4141 tgatgatgat attaacgcat ttcatgagtt aaataatctt ggtgtcatac tggatttacg
     4201 cgtcgtagcg tcagatccct caatcaatat tatcgacaaa ataaacgaac agttaatcgc
     4261 aaattaaata atagcgcctg tgtacatatg ctttaacagg cagggatgac gacacttaaa
     4321 aggtgacaaa tcatggaaat aagtacccta caaatcattg ctatatttct tttttcctgt
     4381 attgccggaa tgggcagcgt gctggatgaa tttcagactc atcgcccgtt aattgcctgt
     4441 acggtgattg gtttaattct cggtgattta aaaaccggaa taatactcgg cggtacgctg
     4501 gaattgatag ctctctgctg gatgaacgtc ggcgcggcgc aatctccgga ttctgcactc
     4561 gccagcataa tctccgccat tctggttatc gttggtcagc aaagcatcgc caccggaatc
     4621 gccatcgcgt tgcctgtggc tgcggcaggc caggtgctga cggtgtttgc ccgtaccatc
     4681 accgtagtgt tccagcacgc ggcggataaa gcagcagaag aggcgcggtt tcgcactctc
     4741 gatattctgc atgtctccgc gcttggcgtg caggcgctgc gcgtcgccat tccggcactg
     4801 attgtctcac tgttcgtcag cgccgatatg gtcagcaata tgttaaacgc cattcccgaa
     4861 tttgtgaccc gaggactaca gattgctggc ggttttatcg tggtggtcgg ttacgccatg
     4921 gtgctacgta tgatgggcgt gaaatatttg atgcctttct ttttcctcgg tttcctcgca
     4981 ggtggctacc tcgatctcag cctgctggcg ttcggtggcg tcggcgtgat catggccctg
     5041 gtctacatcc agctaaatcc acagtggtgt aaagctgaac ctcaactcca ggccaccgcc
     5101 tccaccgccc ttgaccaact tgacgattaa cggagccatc atggaacaga gaaaaattac
     5161 acgcagcgat ctggtgagca tgtttctgcg ctccaacctg caacaggcgt cctttaactt
     5221 tgaacgtatt cacgggctgg gcttttgcta cgacatgatc cccgccatca agcgacttta
     5281 cccattaaaa gaggatcagg ttgcggcgct caggctacac ctggtgttct tcaataccac
     5341 gccagccgta tgtggcccgg tcatcggcgc ggaaattgat gacggtacca tcaacggcat
     5401 caaagtcggt ctgatgggac cattggcagg agttggcgat ccactggtct ggggaacgct
     5461 gcgcccgatt accgccgcgc tcggcgcatc tctggcactt tcgggcaaca ttctcggccc
     5521 gctgctgttc ttctttattt tcaacgcggt gcgtctggcg atgaagtggt atggcctaca
     5581 gctcggcttt cgcaaagggg tgaatatcgt cagcgatatg ggcgggaatg tgctgcaaaa
     5641 actcaccgaa ggcgcgtcga ttctcggact gtttgtgatg ggcgtgctgg tgaccaaatg
     5701 gacgtcaatc aacgtaccgt tggtggtttc acaaacgcat gccgccgatg gctccaccgt
     5761 caccatgacc gtgcagaaca ttctcgacca actttgccct ggtttgctgg cgctcggtct
     5821 gacgctacta atggttcgtc tgctcaacaa aaaaattaac ccggtatggc tgattttcgc
     5881 cctgtttggc ttagggatta tcggcaatgc tctgggcttc ctgtcctgat tcttcgcccc
     5941 ggcacgactg ccgaggccat cgctcaacat gaggtggttt atgaaaacaa cagctctgcg
     6001 tctttatggt aaacgtgatt tacgcctgga aacctttgac cttcctgaaa tgcaggagga
     6061 tgaaatcctc gcaacggtgg tcactgacag cctgtgcctc tcttcctgga aagaggccaa
     6121 tctgggtgaa aaccataaaa aagtacccga cgatgtgacg accaacccaa tcatcatcgg
     6181 ccacgaattt tgcggcgata ttctggccgt gggtaaaaag tggcagcaca aattccagcc
     6241 gggtcagcgt tatgtgattc aggccaacct gcaactcccc gaccgcccgg attgccccgg
     6301 ctactccttc ccgtgggtag acggcgaagc gacgcatgtg gttattccca acgaggtcat
     6361 ggaacaatat tgcctgctgg catacgacgg cgaaacctat tttgaaggct cactggttga
     6421 accgctttcc tgcgtgattg gcgcgttcaa cgccaactat catcttcagg aaggtagtta
     6481 taaccacacg atggggattc gcccgcaagg gcacacgctg atcctcggcg gcaccggacc
     6541 aatgggactg ttggcgattg attatgcgct gcatggacca gttaacccgt cgctgctcgt
     6601 cattaccgat accgacaacg acaaattgag ttatgcgcgc aagcactatc catcagaacc
     6661 gcaaacactg attcattatc tcaatgccac cgatgcagca tttgatacgt taatggcgct
     6721 gagtggcggt cacggcttcg atgatatttt cgtctttgtg cctaatgaag gactggtgac
     6781 tctcgcctct tccttgctgg cgacagatgg ttgcctgaat ttcttcgccg gaccgcagga
     6841 taaacatttc agcgcgccaa ttaatttcta cgatgtgcat tatgcattta cccactacgt
     6901 gggcacgtca ggcggcaata ccgacgacat gcgcgcggcg gtcaaactga tcgaagagaa
     6961 aaaagtgcag gccgcaaaag tggtaacaca tattcttggg ctgaatgccg cgggcgaaac
     7021 cacgcttgaa ttgcctgccg tcggcggcgg caaaaagctg gtgtataccg ggaaatacct
     7081 gccgctgacg tcactcacgc agattcagga tcaagcactg gcggcgattc tggcgcgtca
     7141 tcaggggatc tggtccggtg aggtggagca atacctgctc gcccatgcag aggcaatttc
     7201 ccatgattaa tcgcgctact taactctgca tgtcgctggc tggtcgcccc agcaacttcg
     7261 cgaccggatg tgcggctgat tgctgaagca gcacaatttg cacgagaagg ataaaaaata
     7321 tcagcgcctt aaggggcgct caaattggtg gcaaagtaca cattgtttat gccggatgcg
     7381 gaatgaagcc ttattcggtc tacataattt tgaaaatcca atatattgca gttatctggt
     7441 aggccaggta agcgtagcgc atcaggcaat gctgtgtttg tcatcagtct cagcgcccgg
     7501 tgcaatacgt gacatcgtga gccatgtcag taagtggtta aattcgcagt ggtagcatgc
     7561 cttccgtgat ttcttactaa ccggagaagt aatggaactg cttttattga gtaactcgac
     7621 gctgccgggt aaagcctggc tggaacatgc actgccgcta attgctgaac agttgcaggg
     7681 tcgccgctca gcggtgttta tccctttcgc tggcgtaacg cagacctggg atgattacac
     7741 agagaaaacg gctgcggttc tcgctccgct gggtgtttct gtcaccggta ttcatagcgt
     7801 tgtcgatccc gttgccgcga ttgaaaatgc ggaaattgtg attgtcggcg gcggtaatac
     7861 tttccagttg ctgaaacagt gccgcgagcg cgggctgctg gctccaatta ctgacgtggt
     7921 taaacgtggc gcgctgtata ttggctggag cgcaggcgct aaccttgctt gcccaaccat
     7981 tcgtaccacc aacgatatgc cgattgtcga tccgcaaggt ttcgatgcgc taaatctgtt
     8041 cccgttgcaa atcaacccgc acttcaccaa cgcgctgccg gaaggccata aaggtgaaac
     8101 ccgtgagcag cgtattcgcg aactgctggt cgtcgcgcca gaactgacga ttattggtct
     8161 gccggaaggt aactggatca cagtgagtaa aggtcacgct acgctgggtg gcccgaacac
     8221 cacttatgtg tttaaggctg gtgaagaagc ggttccgctg gaagctggtc accgttttta
     8281 agtgaattgc cggatgcatg atccggcatc ttgttcatag tgcctgatgc gacgcttgac
     8341 gcgtcttatc aggcctacag gattcacaat attaatactc gtcccgtcct tcatcttcat
     8401 ccggctgttc cagcacactg taagccaccg aacaaaacag cgagttcagg cgctgcatat
     8461 ctcccagtaa gcctaaatgt agcgaactgg tttcaatgct ctgcacgttt tgctgatgca
     8521 ggcgatcgac gtgggcgtgg gaatagcggc gattaagaat gcgaaaacga tgtttgctgc
     8581 gacgcaaacg acgagcgctg gtgacatcgc cagagaagaa caccgacatt gccagcttta
     8641 aattactgag caattgctca tagagcgcat ccagttcctt caacccatca agcgaaaatg
     8701 cccgccgtgc tgccagcgat ttatcggcaa tttcgctgcc catgcgctcg acgatatcgg
     8761 aggcctgttc aaggttgagc gacatttcga tgatctccgc ccagcggcgc gactcttcct
     8821 ccgccaactc ttcttttggc atccgcgcca gatacagctt aatggcggtg tagagaacgt
     8881 tgatatcatc tgccagctta cgcagctctt tctcctgccg tggctcgccg tgcatcactt
     8941 tattcagccc ttccatcatc tgttccatgg cgtcgccaat gcgcagggtt tcgcgcgcgg
     9001 cgttcgccag agcaagcgtg ggcgtgtcca gcgcgctgac atccagatgt ttaggccgta
     9061 gctgggtatc cagttccggt tcatcgcgaa taatcgtttt gcaaaaccgt gccatcgggt
     9121 caacaaatgg cagcatgacc aggcaacgta caaggttgta gaagacgtgg aaatagatca
     9181 ccagttccgc ttttggcaat gacagcttcc ccattgtctc tgccagcaaa tggacaaacg
     9241 gcaggataat caggctaccc accagcttaa acagcagact acccagcgcg acacggcggg
     9301 ctgcggcatt ggcggcactg ttgttgagca tcgccagcag gccggaaccg aggttagctc
     9361 caatcaccag acagagcgcc acggggaagg agataatgcc tgcagcggtc agagtcgcgg
     9421 tcagcagtac agcagcaagg ctggagtagc tgataatggc gaacatcgcg ccaatcagcg
     9481 catccagcag aatatcgccg gtcagcgagg caaagatcac ctgaacgccg tttgcctggg
     9541 tgatcggcgt tacggcctgc acaatcaact ccagcgccag caaaatcagc ccaagaccaa
     9601 taccgacgcg gcccagttgc ccggcgcgtg actgtttgcg tccgaggaaa aaaatcacgc
     9661 cgataaaaat aagtaacggt gacagccagg ataagtcgaa ggtgagaata cgcgccatta
     9721 gcgccgtccc gacatctgcc cccagcacaa tgaccagagc cggtgcgagg gctaccagat
     9781 cctgagcgac gaacgaggtc accagcatgg tggtggcatt actgctctgt accagtgcgg
     9841 taacgccgat ccccgcgcaa aaggcgagcg gctttttttc gacgctccgg ctaaggacag
     9901 tacgcaaacg cgcgccgaag acgcgcatta cgccggttcg aacaatatga gtcccccaga
     9961 ccagcagggc gacggcagaa agcaggtgaa gcagcgttaa cacgagttca gattctcctt
    10021 atcgttatat gttcctgaag caattgaatg accaaaggac cggaaacacg ctgactggca
    10081 tctttactgt gccttttgac attcgttttt ccggctaaga ttattccagc ctgtttccag
    10141 tataagtgtt tcatattaat aaagagacag ggcactcatt gagcgccctg tttgttgtaa
    10201 ggaaaagtga cagatttgtg aatcagtccg cgtcataccc cagattcggt gccagccagc
    10261 gctcaacatc ggagacgctc atacctttac ggcgggcatg gtcttcaacc tgatcgcgct
    10321 gaatttgcgc caccgcgtag tacttgctgt ccggatgact gaaataccaa cccgaaaccg
    10381 atgcaccagg ccacatcgcg aaagattctg tgagtttcat gccagtatgt ttttccactt
    10441 ccagcagctc ccagatggtg gctttttccg tatgttccgg gcaggccgga tagcccggtg
    10501 ccggacggat gccctggtag ttttcgcgga tcagttcttc gttgctgagg ttctcgttcg
    10561 gcgcatagcc ccagtagact ttacgcacac gctcatggag atactccgca aaggcttcgg
    10621 ctaaacggtc ggcaagcgct ttcaccatga ttttgttgta atcatcgtgc tgcgcttcaa
    10681 aggcatcagc cagtgcgtcc tcttccagcc cgccagtcac ggcaaatgcg ccgatgtaat
    10741 ctgctttacc agaaagcttc ggcgcaacga agtcagcgag acagtagtta gcgaatcccg
    10801 ttttttcggt ctgttgacgc agatggtggc tgacgttgat cacatgggtg cgcgtttcgt
    10861 cacggtagat ttcaatgtca tcgcccacac ggtttgccgg gaacaggccc accacgccac
    10921 gcggattcag cgttttctcg gcgcttaatt tatccagcat gtcgttggcg tctttaaaca
    10981 gccgctgcgc ctcaacgccc accacttcat cttccagaat gcgcggatac ttcccggcca
    11041 gcgaccaggt cataaagaac ggtgtccagt cgatgtaatt acgcagcgtt tcgatgctgg
    11101 cttccacttc ctgcacgccg agacggtgcg ccaccggcgg cgtgtaagcc tgccagtcaa
    11161 atgcgaaatc gttatcgcgc gccgcttcca gcgtgaccgg tggtgtgcgt ggtttcttgc
    11221 gcccgtgctg aatacgcacg gtttcgtact ccttgcaggt acgagcgaca aaatcatcac
    11281 gctgagtatc ggaaagcagc gccgctacca caccaacggt gcgcgaggca ttctgcacat
    11341 acaccgtagg gccgctgtag ttctgctcga ttttcaccgc cgtgtgcgct tttgaggtgg
    11401 tcgcgccgcc aatcagtaac ggaatggcga agccctgacg ctccatctct ttcgccacgt
    11461 taaccatctc gtccagcgac ggcgtgataa gccccgaaag gccaatcaaa tcagcattca
    11521 cttctttggc gatacggaga attttttccg caggcaccat aacgccgaga tcgacaattt
    11581 cgtagttgtt acattgcagc accacaccaa cgatattttt accgatgtcg tggacatcgc
    11641 ctttcacggt ggcgatcacc atcttgccgt tggttttacc ctgctctttg ctggcttcaa
    11701 taaacggttc gagatacgcc actgcctgtt tcatgacgcg cgccgatttg accacctgtg
    11761 gcaggaacat tttgccttcg ccaaacagat cgccgaccac gttcatgccg tccatcaacg
    11821 ggccttcaat cacttcaatc gggcgcgtag cctgctggcg ggcttcttca gtatcctgct
    11881 cgataaactc ggttatgcct ttgaccagcg agtattccag acgtttattc acgtcccacg
    11941 agcgccactc cgcttgctgg gcgttagcag tgtcgtccgc tttactgccg cgatatttct
    12001 cggcaagctc cagtaaacgc tcagtgccat cgtcacgacg attaagaatc acatcttcca
    12061 ccgcgtcgcg cagttcagcg ggtaggtcgt cgtaaatcgc cagttgcccg gcgttgacga
    12121 tccccatatc catgccattg cgaatagcgt agtagaggaa cactgcgtga atggcttcgc
    12181 gcaccggatc gttgccacgg aatgagaaag aaacgttaga tacgccgccg gaaatcagcg
    12241 cgtgcggcag ttcgcgtttg atgtcttcac acgcgccgat aaagtcctgc gcgtagttgt
    12301 tgtgctcttc aataccggtg gcgaccgcga agatgttcgg gtcgaagatg atatcttctg
    12361 gcgggaagcc aacctcttcg gtgaggattt tgtacgcccg acggcaaatc tcgattttcc
    12421 gtgcgcgagt atcggcctgt ccctgttcgt caaaggccat caccaccact gccgcaccgt
    12481 agcgacgcaa caatttcgcg tgatggataa aggcgtcgac gccctctttc atcgagatag
    12541 agttaacaat gcctttgccc tggatacact tcagaccttt ttcaatgaca tcccattttg
    12601 aggagtcgat cataatcggc acgcgagcga tatccggttc accggcaatc agattgagaa
    12661 aacgcaccat cgccgcttcg gcatcgagca tcccttcatc catgttgata tcgataatct
    12721 gcgcgccgtt ttccacctgc tgacgcgcca catccagcgc ctcgctgtat ttctcttctt
    12781 tgatcaggcg cttgaactta gcagagccgg tgacgttggt gcgttcaccc acgttcacaa
    12841 acaggctatc gtcgccaatg ttcagcggct ccaggccaga caaacggcag gctacgggaa
    12901 tttccggcag tttgcgcggc gctaatcctt ctactgcacg actcatcgct gcaatatgtt
    12961 gcggcgtggt cccgcagcaa ccaccgacga tattgagaaa gcccgcttgc gcccattcac
    13021 gtatctgttt tgccatcgtg tcggcgtcga gatcgtattc accaaaggcg ttaggcagcc
    13081 cggcgttcgg gtgggcggtg acgtagcatt ccgcaatccg tgatagttcc tgcacgtact
    13141 ggcgcagttc atcgggcccc agcgcacagt tcaggccaaa ggtcagcgct tcggcgtggc
    13201 gcaatgagtt gtaaaatgct tcggtggtct gcccggagag cgtgcgcccg gaggcgtcgg
    13261 tgatggtgcc ggagatcata atcggcagct caacgcccag cgcttcaaac tccgttttca
    13321 ccgcaaagac cgccgctttg gcgttaaggg tgtcgaaaac ggtttcaatc aggatcagat
    13381 ccgcaccacc ttccaccagc gctttggtgg attcgcgata ggccgccgcc agctggtcaa
    13441 aagtgatgtt gcggaatgcc ggatcgttga cgtccggaga aatagacgcc gtgcggttgg
    13501 ttgggccaag aacaccggca acgtagcgcg gtttctccgg cgtgcgcgcg gtccactcgt
    13561 cagcacaggc gcgcgccagt ttcgccgccg caaagttgat ttccgccgac agggattcca
    13621 tctggtaatc cgccatcgca atggtcgtgg agttgaaggt gttggtttcg atgatatccg
    13681 cgcccgcttc aaagtaggcg ttgtggatag cggcgatcac ttccggttta ctgagtacca
    13741 gcaggtcgtt gttgcctttg aggtcgcatg gccagtcggc aaagcgttca ccacgaaaat
    13801 cggcttcgtt cagtcgatag ctctggatca tggtgcccat accgccgtcc aacaccagaa
    13861 tacgttcatt taactgcgca cgcagttgtt ccactttgct gctcacactt gctcccgaca
    13921 cgcttaaccc agatttaata aaaattcaac aaaccatact ggaataaacg catctgtggt
    13981 aaaagcgacc accacgcaac atgagatttg ttcaacatta actcatcgga tcagttcagc
    14041 aactattgca ttagctaaca ataaaaatga aaataatttc cacgatacag aaaaaggagg
    14101 ctgtcatggt cgcacccatt cccgcgaaac gcggcagaaa acccgccgtt gccaccgcac
    14161 cagcgactgg acaggttcag tctttaacgc gtggcctgaa attactggag tggattgccg
    14221 aatccaatgg cagcgtggca ctcacagagc tggcgcaaca agccgggtta cccaattcca
    14281 cgacccaccg cctgctaacc acgatgcaac agcagggttt cgtgcgtcag gtcggcgaac
    14341 tgggacactg ggcaatcggc gcacatgcct ttatggtcgg cagcagcttt ctccagagcc
    14401 gtaatttgtt agcaattgtt caccctatcc tgcgcaatct aatggaagag tctggcgaaa
    14461 cggtcaatat ggcggtgctt gatcaaagcg atcacgaagc gattattatc gaccaggtac
    14521 agtgtaccca cctgatgcga atgtccgcgc ctatcggcgg taaattgccg atgcacgctt
    14581 ccggtgcggg taaagccttt ttagcccaac tgagcgaaga acaggtgacg aagctgctgc
    14641 accgcaaagg gttacatgcc tatacccacg caactctggt gtctcctgtg catttaaaag
    14701 aagatctcgc ccaaacgcgc aaacggggtt attcatttga cgatgaggaa catgcgctgg
    14761 ggctacgttg ccttgcagcg tgtattttcg atgagcaccg cgaaccgttt gccgcaattt
    14821 ctatttccgg accgatttca cgtattaccg atgatcgcgt gaccgagttt ggcgcgatgg
    14881 tgattaaagc ggcgaaggaa gtgacgctgg cgtacggtgg aatgcgctga ctttttctgg
    14941 cgggcagagg caatattctg cccatcatac ctgagtggca atagaataag ggtgtctgtt
    15001 aatcgcattg acgccaaaat aacttaatgt catacacttc acacactttt attaactcag
    15061 cattattttt aaacatcaaa ccacttaatt attaacaaaa tcgcaagatc tcattccacc
    15121 gaaactttta cggctgcatt atgagattga gcgatttatg atattttttt agttgtaatc
    15181 atattaaatt ttttcactgt tctgatagtt aaaattcaag acatcaataa acaatgagat
    15241 atttaaatga ttactcgtat tcctcgtagt tctttctctg caaatattaa taatacagcc
    15301 cagacaaatg aacaccaaac cctgagtgaa ttgttttata aagaactcga ggataaattt
    15361 tctggcaagg agctggcgac gcctctatta aaaagcttct cagagaactg tagacaaaat
    15421 gttcgtcata tctttagcaa caaggatttt gtcattaaat tttccacgtc cgtcttacaa
    15481 gctgataaga aagaaattac gatgattaat aaaaacgaaa acacgacact cactcaaacc
    15541 attgctacaa tatttgaaga atacctaatg gaaattttac ctcaacgctc agacactctt
    15601 gataaacaag aattaaacca aaaatcagat agaaaagaaa aagaattccc aagaattaaa
    15661 cttaatggtc aatgttattt cccggggcgc cccaaaaccg tatagtatac cgacacattg
    15721 ctgcacaata tattaatgat atttatcaga atgttgatta caaaccccat caagatgatt
    15781 actcttcagc tgaaaaattt ctcactcact tcaacaaaaa atgcaaaaac cagactttgg
    15841 cgttgatttc cagccgtcct gaggggcgtt gcgttgctgc ctgcggtgat ttcgggctag
    15901 ttatgaaagc atattttgac aagatggaat caaatggcat cagtgttatg gcagccatat
    15961 tactggtgga taaccatgct ttgacggtcc ggctaagaat aaagaacaca actgaaggat
    16021 gtacccatta cgtggtttcg gtttatgatc ctaatgtaac taacgataaa ataagaatta
    16081 tgagcgaaag caaagaggat attaaacact attctctgat ggattttatg aatgtagatt
    16141 atagcctcct gaaatggtca aatgatcatg ttattaacca atctgttgca ataattccag
    16201 cacttccgaa agaacagcta ttgatgttaa aaggaactgt ggatgaaata acccctccat
    16261 tatcaccagc aacgatgaat ttgctaatgg caattggtca gaatcaccaa cttacgcaac
    16321 tgatgattca gctccagaaa atgccagaac tacatagaac agaaatgttg actgcctata
    16381 atagtattaa cctccccggt ttatatttgg ctataaatta tggcaatgcg gatatcgttg
    16441 agactatttt caattcattg tcagaaacag gatatgaagg gttactctcg aaaaaaaatc
    16501 tcatgcatat tctggaggca aaagataaaa atggtttttc tggattattt ttagcgatat
    16561 cacgtaagga taaaaatgtt gtaacctcga ttctgaacgt cttacctaaa ctggccgcaa
    16621 cacatcattt agataatgaa caggtgtata aattcctgag tgccaaaaat agaacgtcca
    16681 gccatgtttt ataccatgtt atggcgaatg gtgatgccga catgctgaaa atttttttgg
    16741 tcgcgttacc tttgttaatt cgcacatgtc atttgactaa agaacaggta ctcgatctcc
    16801 tgaaggcaaa ggatttttat ggttgcccaa gactatacct ggcgatgcaa aatggacata
    16861 gcgatatcgt gaaagttatt ctcgaagcat tgccctgtct ggcccaggaa attaacattt
    16921 cagcttccga tattgtagat cttctgaccg ctaaaagtct tgcgcgcgac acgggtttgt
    16981 ttatggccat gcagcgcgga catatgaacg ttattaatac tatttttaac gcattaccca
    17041 ctctttttaa tacgtttaaa ttcgataaaa aaatatgaag cccctcctcc tggcaaataa
    17101 ttctaatgaa tatcccggtt tgttttcagc gatacagcat aaacaacaaa acgttgtaga
    17161 gacggtttat cttgctttat ctgaccatgc acgcctgttt ggatttaccg ctgaagatat
    17221 tatggatttt tggcaacaca aagcgccaca aaaatactct gcctttgagt tggcttttga
    17281 atttggtcac cgggttattg ctgaattaat ccttaataca ttaaataaga tggctgaaag
    17341 ctttggcttt acggataacc ctcgatacat tgcggagaaa aattatatgg aagctttact
    17401 caaaaaagca tctccccata ccgtacgcta aatctttgcc tgcgccgata cgcataaaca
    17461 tcttccacat gcccttcacg tatgcggttt tgtagtgcgc gccagtaatc agcgcggaac
    17521 aggtcggcgt gcatctcttc aaacagcgga ccaatacgcg ggtcggcgca tagccagtgg
    17581 cgaaactctt ccgggaaaac atcgcccggc gagacgctgt accacggttc gctggcaagt
    17641 tcgtcttccg gatagcgcgg cggcgggatg tcgcggaaat tcacttccgt catgtagcaa
    17701 atttcatcgt aatcataaaa aaccacacgc ccgtgacggg tgacaccgaa gtttttaaac
    17761 agcatgtcgc cagggaaaat gttagcagcg gcaagctggc gaatagcgtt accgtattct
    17821 tcaatggcgt cgcgcaactg ctgaccttcc acttgttcca gccagatatt gagcggcacc
    17881 atccgccgct cgattataga gatggcgaat tacaatctgc tcaccaagat cggtgatttt
    17941 ttccgctgct tcctggagca gtaattccat taataccggg gaaatatgcc gcttctccag
    18001 cacaaagttt tcaaactcct gggtgtccgc cattcggccc acacggtcgt gctctttcac
    18061 cagttgatag caggcacgaa cgtgagcggc agacatctct ttctgcggcg cgaacttgtc
    18121 tttgatgact ttgaataccc gatcaaagcc cggcagcgta aacaccaaca tcaccattcc
    18181 acgaataccc ggcgcttcaa tgaactgctc attacagccc tgtagataaa cgagatattc
    18241 gcggtagctt tcggttttgg cgtgcttctg gcagccgata gccatataca attcagcagt
    18301 ggttttacct ggcagaattt cccgtagcca ctcgaccagc gctgcgggca gcggtgcata
    18361 aaccataaaa taagaacgcg caaagccaaa aacaatgctc gcttcggcgg tcgtcgtcag
    18421 gcaggtatca ataaataact cgccgtcgtc cgtctggtgg atcggcagta agaagggcaa
    18481 cgtgccggaa ggcgtgatca gtttgcccac cagccaggcg gctttattgc ggtaaaacag
    18541 ttcgttcgcc acctgtaaat gactttccgc gaggttgtct gtccccagcg tttccgtcag
    18601 atggcgaatg atgtaatgaa tgtcacggct tttattctgc cagcgcaggc gcagcggcag
    18661 gtcgctgata acgcgcatca gtagagattc ccagccgtga tcggggtgaa agtctttcgc
    18721 cagcgggcgg ggaatggtac gaaagcggcg ctctggctga gagctaaaga taaaaagccg
    18781 ctcgggagta agcgagcggt ggtcaaataa ccgacagtac acggagttaa aaaagctctc
    18841 cgcaatctcg aagcgcgggt aatccggcaa cagccgcgtg taatgctctt taacgcgcag
    18901 taaaaatgcc gcgtccgtac tttggccgtt agtaatgcag cgcagttgct ccacgaccag
    18961 accaacgtga tgatcgtaaa gatggatacg gtttttcatc gcctgctgga cagcatgcca
    19021 gtcggcctgt tcgaaacgct gctgcgcccc ggaagtcact tcgaggaatc gaccatactg
    19081 agcgtcgaag ccttgcaaaa tggtttgagc aatcagtaat tccaggccac gcggcattta
    19141 gcgccctcat caggagcaga gaattgccgg atgcggcgta aacgccttat ccagcctacg
    19201 ttcggcaacg gttgtaggca tgataagacg cgcaagcgtc gcatcaggca tcgtgcaccg
    19261 attgccggat gcggcgtaaa tgccttatcc ggcctacagt cagcaacgtt tgtaggttgc
    19321 ttagaactgc gattcttcag tggagccagt cagcgcggtg actgaagacg tgccgccctg
    19381 aataatagtc gtcactttat cgaagtaacc tgtacccact tcctgctggt gagatacgaa
    19441 ggtatagcca tctttcgcgg cggcaaattc cggctgctgc actttctcaa catagtgctt
    19501 cataccttcg ccctgcgcat aagcgtttgc caggtcgaac atgttaaacc acatgctgtg
    19561 gatacctgct agggtgatga actggaactt gtagcccata tccgacagct gctgctggaa
    19621 gctggcaata gttttgtcgt cgaggttttt ctgccagttg aacgacggcg agcagttata
    19681 agccagcagt ttgcccggat atttcgcgtg gatagcttgt gcaaagcgac gcgccagttc
    19741 cagatccggc gtggaggttt cacaccagac caggtcagca tatggcgcat acgccaggcc
    19801 acggctgatc gcttgctcaa tgcccgcatg agtacggaag aagccttcac tggtacgctc
    19861 gccggtaata aattcgctgt catacgggtc gcaatctgag gtgatcagat ccgccgcatc
    19921 agcatcggta cgtgcaacca gcagggttgg aacgcccgtc acgtcagctg ccagacgcgc
    19981 cgcgaccagt ttctgaatag cttcctgagt tggcactaaa actttgccgc ccatgtgacc
    20041 gcatttcttc actgacgcca gctgatcttc gaagtgaact gccgcagcac cggcttcaat
    20101 catcgctttc atcagttcaa aggcattcag gacaccgcca aaaccggctt ccgcatcggc
    20161 aacgatcggc aggaagtaat cgacatagcg cggatcgccc ggctcaatgc ccacggacca
    20221 ttggatctga tcggcacgac ggaaggtgtt gttgatccgc tccaccacag ctggcaccga
    20281 gtttgccgga tagagcgact gatccggata catgctggcc gccaggttag cgtccgccgc
    20341 tacctgccat cccgacagat agactgcttc aatacccgct ttcgcctgtt gcagcgcctg
    20401 accgccagtc agtgcaccga ggttgttgat gtagcctttt ttcgactcac cgtgcagcag
    20461 acgccacatt ttcgctgcgc ccagttgcgc cagcgtgcat tcaggattga ctgaaccgcg
    20521 taatttcacc acatcttccg cactgtatgg gcgagtaatg ccttcccaac gcggttgagt
    20581 ccactctttc tgtaattctt caatttgttg tgtacgggtt ttcatgtgca gatgctccat
    20641 agttatgtgg tggtttacgc taacaggcgg tagcctggca gggtcaggaa atcaattaac
    20701 tcatcggaag tggtgatctg ttccatcaag cgtgcggcat cgtcaaaacg gccctgggag
    20761 aaacgttctt cgcccagttc gctggcaatg actttcatct cttcgcccag catctggcgg
    20821 aacaaggctt tggtcaccgg tttgccattg ctcaacgttt tttgatgatg gatccactgc
    20881 cagatcgagg tacgggaaat ttcagccgtc gccgcatctt ccatcaggcc ataaatcggc
    20941 acgcagccgt tgccggagat ccacgcttcg atgtactgca cagccacgcg aatgttagcg
    21001 cgcatacctt cttcggtgcg ttcaccatca caaggtgcca gcagctgatc ggcagtaatc
    21061 ggcgcgtctt gttcgcgcaa tacttcaagc tgatttttac gggagccgag aatgtcgttg
    21121 aataccgcca ttgccgtgtc cgcaaggcct gggtgagcga tccatgtgcc atcgtgaccg
    21181 ttattggctt ccagcgcttt atccgctttt actttgttga gcacctggtt attacgctct
    21241 tcatctttgc tcggaataaa cgcggccatg ccgcccatcg caaaagcacc gcgtttatgg
    21301 caggttttaa tcagcaggcg tgagtaagca ttcaggaatg gtttatccat cgtcacggcc
    21361 tgtctgtctg gcaggacacg atcgggatag tttttcaacg ttttgatata gctgaagatg
    21421 taatcccagc gaccgcagtt cagaccaaca atatggtcgc gcagcgcgtg aaggatttcg
    21481 tccatctgga acacggcggg cagcgtttca atcagcaacg tcgccttgat ggtgccgcgc
    21541 ggcagattaa agcgatcttc tgcatagctg aagacttcgc tccaccaggc cgcttcctgc
    21601 taggactggg ttttcggcag atagaaatag ggaccgctgc cctttgccaa cagtgcctga
    21661 tagttgtgga agaaatagag cgcaaaatca aacaggctac cggggattgc ctcaccacgc
    21721 caggtgacat gtttttccgg caagtgcaga ccgcgtaccc gacaaatcaa aaccgctgga
    21781 ttgggcttga gctgataaat tttgcctgct tcattggtat agctgatggt accgttaacc
    21841 gcatcgcgca ggttaatttg cccgtcgatc actttgttcc agtctggtgc cagtgaatct
    21901 tcgaaatcgg ccataaagac tttcacattg gcgttcagtg cgttgatcac catcttgcgc
    21961 tctaccgggc cagttatctc tacgcggcgg tcttctaagt ccgcaggaat cccgcgaatt
    22021 ttccaatcag catcgcgaat ggaagccgtt tccgaaataa aatcaggcaa cgttccgtta
    22081 tcaatatctt gctgctgctg aatgcgcgct gccagaagtt tattgcgttg tggcgtaaaa
    22141 tgcgtcacca gctcagtcag aaattctacc gcttcggcag taagaatttg cttctcctgc
    22201 tcgccatacg gccttgtgaa agccagttca tcggttgttg ttgcctgttc agtcatcgtg
    22261 cagctcctcg tcatggatcc gaaaacttcc cctctgtgaa cgaaggaacg ttgtgcgctt
    22321 ttcgttcagc acaactaaga ctactcattt aaaatgcaaa atcaaaaaca atttccattt
    22381 taattaaaaa tacttgataa cttattgata acaatatgaa taaaatttat tcacttgctg
    22441 aggtgcgttt cggaaataaa aaaggcaccc gaaggtgcct gaggtaaggt gctgaatcgc
    22501 ttaacgatcg actatcacag aagattaatc cagcgttgga ttcatgtgcc gtagatcgta
    22561 tggcgtgatc tgatagacgt aatagttgag ccagttggta aacagcaaat taccgtgact
    22621 acgccagctc gctcgcggtt tgttttgcgg atcgttgtgc gggaaatagt tatacggtac
    22681 atccgggtct agtccggctt ccacatcgcg taaatattcc tgcgccagcg tttgcgcatc
    22741 atattcggga tggcccgtca caaaggcaat gcgcttatct ttgctggcaa acagatatgc
    22801 atccccttct tccgtctctg ccagaatttc cagatcggtg taatcacgaa tcaacgctgc
    22861 cggaaagtca gcatagcgcg aatgcggtgc caggaatgaa tcatcaaagc cacgcgtcag
    22921 aagcgcatga ggatggagaa tatgatgctc gtaaacgcca gagagtttgt cggtgcgagt
    22981 ttgcttagga atgccgtaga ggatattgag cgcggcctgt accgcccagc agacaaacag
    23041 cgtcgaggtg acgtgatctt tcgaccactc cagcacctgt ttgatctgcg gccagtaagc
    23101 gacatcatta aactccacca ggcccagcgg cgcaccggtt acaatcagac cgtcaaagtt
    23161 ctgttcctga atatcttcaa agttacagta gaagttgttc agatgctctg cgggcgtgtt
    23221 gcgcgattca cgggaatcga tgcgcaacag ctgaatatcg acctgcaaag gtgagtttga
    23281 aagcaggcgc agaaactgat tttcagtttc aatcttcttc ggcatcaggt taaggatcag
    23341 aaccttaagt ggacgaattt cctgaccaga cgcacgagaa gttgtcatca caaagacgtt
    23401 ttcttcacgc aagaaattga cggcgggtag ctcgtccggc acacgaatcg gcataacctg
    23461 attacctcac tacatacgct tatacgttta gacatccaga tagctgaaga taaccagaaa
    23521 atttgccaat gtcgagcctg catgttgaag gtgagaaagt ttcaaggaaa aggctacgtt
    23581 agaatataag aatgacgaaa aggagagaaa atggttatta gtattcgccg ctcacggcat
    23641 gaggaagggg aggaactcgt tgcgatttgg tgtcgatctg tcgatgccac ccacgatttt
    23701 ttatcagcag agtatcgggc cgagctggaa gagctggtgc gttcctttct gccggaagca
    23761 ccgttgtggg tcgcggttaa tgaacgggag cagccggttg ggtttatgtt gttaagtggg
    23821 cagcatatgg atgcgctgtt tatcgatcct gatgtgtgcg gctgcggcgt gggtcggatg
    23881 ctggtggagc atgcgctctc gatggcaccg gaactgacaa ccaacgttaa tgagcaaaat
    23941 gagcaggcgg ttgggttcta taagaaggtg ggttttaagg ttacgggacg ctctgaggtg
    24001 gacgatttgg ggaaaccgta tccgttgctg aatctggcgt atgtggggga ataagccgcc
    24061 tctgccgtgc ctgcccaagc tggagaagac gcgccagcgt cgcacccggc atttttttca
    24121 ccaa
//
LOCUS       DAETQL010000062        22412 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.062, whole genome shotgun sequence.
ACCESSION   DAETQL010000062 DAETQL010000000
VERSION     DAETQL010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.062"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            82..2682
                     /locus_tag="K0W65_002670"
     CDS             82..2682
                     /locus_tag="K0W65_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HBI3095877.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNRGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2673..3656
                     /locus_tag="K0W65_002671"
     CDS             2673..3656
                     /locus_tag="K0W65_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3095878.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3754..4294
                     /locus_tag="K0W65_002672"
                     /pseudo
     CDS             3754..4294
                     /locus_tag="K0W65_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4302..4817
                     /locus_tag="K0W65_002673"
     CDS             4302..4817
                     /locus_tag="K0W65_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI3095879.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4783..5520
                     /locus_tag="K0W65_002674"
     CDS             4783..5520
                     /locus_tag="K0W65_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBI3095880.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5631..6641
                     /gene="pyrD"
                     /locus_tag="K0W65_002675"
     CDS             5631..6641
                     /gene="pyrD"
                     /locus_tag="K0W65_002675"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBI3095881.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6815..7357
                     /gene="zapC"
                     /locus_tag="K0W65_002676"
     CDS             6815..7357
                     /gene="zapC"
                     /locus_tag="K0W65_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBI3095882.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="K0W65_002677"
     CDS             complement(7354..8463)
                     /gene="ycbX"
                     /locus_tag="K0W65_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBI3095883.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8707..10815
                     /gene="rlmKL"
                     /locus_tag="K0W65_002678"
     CDS             8707..10815
                     /gene="rlmKL"
                     /locus_tag="K0W65_002678"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBI3095884.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10827..12734
                     /locus_tag="K0W65_002679"
     CDS             10827..12734
                     /locus_tag="K0W65_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3095885.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12864..14117
                     /gene="pqiA"
                     /locus_tag="K0W65_002680"
     CDS             12864..14117
                     /gene="pqiA"
                     /locus_tag="K0W65_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBI3095886.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14122..15762
                     /gene="pqiB"
                     /locus_tag="K0W65_002681"
     CDS             14122..15762
                     /gene="pqiB"
                     /locus_tag="K0W65_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBI3095887.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15759..16322
                     /gene="pqiC"
                     /locus_tag="K0W65_002682"
     CDS             15759..16322
                     /gene="pqiC"
                     /locus_tag="K0W65_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBI3095888.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16578..16745
                     /gene="rmf"
                     /locus_tag="K0W65_002683"
     CDS             16578..16745
                     /gene="rmf"
                     /locus_tag="K0W65_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBI3095889.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="K0W65_002684"
     CDS             complement(16815..17333)
                     /gene="fabA"
                     /locus_tag="K0W65_002684"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBI3095890.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17402..19162)
                     /locus_tag="K0W65_002685"
     CDS             complement(17402..19162)
                     /locus_tag="K0W65_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBI3095891.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19347..19799
                     /gene="matP"
                     /locus_tag="K0W65_002686"
     CDS             19347..19799
                     /gene="matP"
                     /locus_tag="K0W65_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBI3095892.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="K0W65_002687"
     CDS             complement(19875..20921)
                     /gene="ompA"
                     /locus_tag="K0W65_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBI3095893.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="K0W65_002688"
     CDS             complement(21277..21786)
                     /gene="sulA"
                     /locus_tag="K0W65_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBI3095894.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22005..>22412
                     /gene="sxy"
                     /locus_tag="K0W65_002689"
     CDS             22005..>22412
                     /gene="sxy"
                     /locus_tag="K0W65_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBI3095895.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT         5721 a         5257 c         5874 g         5560 t
ORIGIN      
        1 tatcgaacca tcaatgacta cggggcggaa acgccgcaac tcaactgtaa atcgtaagcc
       61 gtcttcagtt aagagagcga gatgtataga actcatcgac aacacagcct gttaagctct
      121 ggtggagtgc catcgtttat tggtgggctg gtggtgtttg tgtcggcagc gttcaatgca
      181 caagctgaaa cctggttcga tcctgctttt ttcaaagatg atccctcaat ggtggccgat
      241 ttgtctcgtt tcgaaaaagg acaaaaaata acgccagggg tttatcgtgt cgatattgtt
      301 ctgaatcaga caattgtaga tacgcgcaac gtcaattttg ttgagataac gccagagaag
      361 gggattgccg cctgtttgac gactgaaagc ctggatgcaa tgggtgttaa tactgatgcg
      421 tttccggctt ttaaacaact ggacaaacaa gtgtgtgtgc cattggcgga gattattccg
      481 gatgccagcg taacgtttaa tgtgaataaa ctccgtctgg aaatttcagt accgcaaatc
      541 gccatcaaaa gtaatgcccg tggttatgtc ccccctgaac gttgggatga agggatcaac
      601 gcgctattac tgggatattc gtttagcggg gctaacagta ttcatagcag cgcagacagt
      661 gattctggcg acagctattt tctgaattta aacagtggcg ttaatttagg cccatggaga
      721 ttacgcaaca attcaacatg gagtcgcagt agtggccaaa ccgcagaatg gaagaatctc
      781 agcagctatt tgcagcgggc ggttattcca ctaaaaggcg aactgaccgt aggtgatgat
      841 tatactgcag gcgatttttt cgatagtgtc agctttcgtg gtgtgcagct ggcgtcagat
      901 gacaacatgc tgccagacag cctgaaaggg tttgcgcctg tggtgcgtgg tatcgccaaa
      961 agcaatgccc agataacgat taagcaaaat ggttacacca tttaccaaac ttatgtatcg
     1021 cctggtgctt ttgaaattag tgatctctat tccacgtcgt cgagcggtga tttgttagtt
     1081 gaaatcaaag aagcggacgg cagcgtcaat agctacagcg taccgttttc cagcgtgcca
     1141 ttactccagc gtcaggggcg aatcaaatac gcggtgacac tggcgaaata cagaaccaat
     1201 agtaatgaac agcaggagag caaatttgcc cagaccacgt tgcagtgggg cggaccgtgg
     1261 ggaacgacat ggtatggtgg tggacaatat gctgaatatt accgtgccgc catgtttggt
     1321 ctgggattta accgtggcga tttcggagca atttcgttcg atgcgaccca ggcgaagagt
     1381 acgctggcag accaaagcga acataaaggt cagtcatatc gttttctgta tgccaaaacg
     1441 ctcaaccaat tgggcactaa ttttcaattg atgggctatc gctattcgac gtcgggtttc
     1501 tacacccttt ccgacaccat gtataaacat atggatggct acgaatttaa tgacggtgat
     1561 gatgaagata cgccgatgtg gtcgcgttat tacaatttgt tttacaccaa acgtggcaaa
     1621 ctgcaggtca atatctccca gcaattaggc gagtacggtt cgttttattt aagtggtagc
     1681 cagcaaactt actggcatac cgatcaacag gatcggctat tacagtttgg ctacaacacg
     1741 caaattaaag atctctcgct gggggtttcc tggaactaca gtaagtcccg tggtcaacct
     1801 gatgctgatc aggtgtttgc actaaatttt tccctgccgc tcaatctgtt gctccccaga
     1861 agtaatgata gctataccag gaaaaaaaat tacgcctgga tgacctctaa caccagtatc
     1921 gataacgaag ggcacattac acaaaacctg ggtttaacgg agacactact cgatgacggt
     1981 aacctgagct acagcgtgca acagggatat aacagcgagg ggaaaacggc taatggtagc
     2041 gccagcatgg actacaaagg ggcgtttgca gatgcccgag tgggctacaa ctacagcgat
     2101 aacggcagtc aacaacaact gaactacgct ctttcaggca gtttagttgc ccattcacag
     2161 ggcattaccc tggggcaatc gctgggggaa actaacgtgc tgattgcagc accaggcgca
     2221 gagaatactc gtgtggcgaa cagcaccggg ctgaaaactg actggcgcgg atataccgtt
     2281 gtcccctatg ccacttctta tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa
     2341 cgtaacgtgg atcttgaaaa tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt
     2401 ctggcggagt tcaatgccca tgcgggagca agggtattaa tgaaaacatc aaagcagggt
     2461 ataccgctgc gttttggcgc gatagcgacg ctggacggca tacagactaa tagcggcatt
     2521 attgatgatg atggctcgct ctatatgtcc ggtttgcccg ctcagggagc gataactgta
     2581 cgctggggcg aagctcccga tcaaatttgt catatcagtt accagcttac cgaacaacaa
     2641 attaactctg cgattacgcg aatggatgcc atatgcagat aatctttgga gaaaaatgcg
     2701 tgttattact acgactattt tttgccgccg tcttaatgct atggtgcgct caaactgctg
     2761 cttatatcgg gcagtgtcat accactcagg ggaatccgta tattggcgtc aattttggcg
     2821 ttaaaactct ggatgaagaa gaaaatacgg ctggagtagt taaagacaaa ttttatcagt
     2881 ggaacgaatc gaatgattat tatgtttcct gtgattgcga taaagacaat gtcagaagtg
     2941 gccgatgggc attcgccgcg gattcaccgt tagtctattt aggcgacaac tggtacaaaa
     3001 ttaatgacta tcttgccgcc aaagttttat tgcaggttaa aggcagttct cctactgcgg
     3061 ttcctttcga aaacgtgggc acaggggcag atacacgatg gcatatttgc gatcccggcg
     3121 gtcaacgttt aggtggccag ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat
     3181 tgcagccgtt cgttggttcg gtcgtcattc ctcctatggc gctggcgcga ttatttgaat
     3241 gctacaacat acccgcaggt gattcctgca cgactactgg tacaccggtt ttagtgtatt
     3301 acctgtctgg tacgatcaat tcacttggct catgttccgt caatgccgga gaaacaatcg
     3361 aggttgatct gggcgacgta tttgcggcta actttcgtgt tgtagggcat aagcctcttg
     3421 gggccagaac ggcagaactt gcaattccgg tcaggtgtaa cacgggaaac gcagggttag
     3481 ttaacgtcaa tctgagtctg acggcaacct cagaccccag ctatccccag gcgattaaga
     3541 cgtcacgtcc tggcgtgggc gtggtggtga ccgatagcca gcacaacatt atttcccctg
     3601 ctggtgggac actaccgctc tctattcctg atgatgcaga cagtatccgc gaatgaatgt
     3661 ctatccagtc agcacgacaa gcgtaccacc agaaaccggg cgatttgaag ccacggcaac
     3721 ggtgagaata aattttgatt aacgcgtgaa cgtatgaaga aaaaaacgat atatcagtgc
     3781 gttattttgt tctttagcct tcttaacatc catgtcggga tggctgggcc tgaacaagtc
     3841 agtatgcata tttatgggaa tgtggtcgat cagggctgtg atgtcgccac caaaagtgca
     3901 ttacaaaata ttcatattgg tgattttaat atcagtgatt ttcaggccgc gaataccgta
     3961 agcactgctg ctgatttgaa tattgatatc accggttgtg ccgctggtat tactggcgcg
     4021 gacgtccttt ttagcggcga ggctgacacc cttgcgccga cactgctcaa actaactgac
     4081 acaggcggaa gcggtggtat ggcaacgggg attgccgtgc attcttgatg cgcaaagtca
     4141 gcaagaaatc ccgctcaatc aggtccagcc tcttacgccc ttaaaagccg gggataacac
     4201 actcaaatat caacttcgtt ataagtccac aaaggcggga gcaacgggcg gtaatgcgac
     4261 ggcggttctc tattttgatc tggtttacca gtgaaggagt gatgttgaaa cggataatct
     4321 ggattctgtt cttactggga ttaacggggg gctgtgagct atttgcccat gatggcacgg
     4381 tcaacattag cggatcgttt cgccgtaata catgcgtgct ggcacaggat agcaagcaaa
     4441 tcaatgtgca gttaggcgat gtctcgctga cacgtttttt tcatgggaat tatggcccgg
     4501 agaaatcttt catcattaat ttgcaggatt gcggaacaga tgtcagcacg gttgatgtca
     4561 ccttttcagg aacccccgac ggagtgcaga gcgaaatgct gtccattgaa agtggtacgg
     4621 atgcagccag tgggctggca attgcgattc tggatgatgc gaagatattg attccgctta
     4681 accaggcgag taaagactac agcctacata gcggtaaggt gccattaact ttttatgcgc
     4741 aattgcgacc tgtcaatagc gatgtgcagt cgggtaaagt gaatgccagc gcgacatttg
     4801 tacttcatta tgactaatac gtggaacaga ttagcgctct tgattttcgc cgttttatcg
     4861 ctgctggtgg cgggtgaatt gcaggcagga gtggtggtcg gcggaacgcg atttatcttt
     4921 ccggcagaca gagaatcgat atctatttta ctgactaata ccagtcagga atcttggctt
     4981 attaacagca aaatcaatcg cccaacgcgt tgggccgggg gggaagcgtc gacagtgcca
     5041 gcactattac tggccgctcc gccacttatt ctcctgaagc ccggtactac aggcacgttg
     5101 cgcttgctga gaacggaaag cgacatcttg cctgtggatc gcgaaacgct atttgagtta
     5161 agcattgcca gcgtgccatc cggcaaagtt gaaaatcaga gcgtaaaagt ggcgatgcgc
     5221 tcggtattta aactgttctg gcgacccgaa ggtttgccgg gcgacccgct ggaagcttat
     5281 caacaattac gctggacacg gaattcgcag ggtgtacaac tcactaaccc aacgccttac
     5341 tacattaacc tgattcaggt gagcgtaaat ggtaaagcgt taagtaatgc gggagtcgtg
     5401 ccgcctaaaa gccagcgtca gacaaactgg tgtcaggtaa tagcaccgtg tcatgtcgcc
     5461 tggcggacga taaatgatta cggcgggttg agtgcaaaga aggagcaaaa tctgccctga
     5521 aacaggttct gaaaacgttt gcgttttttt tgccgcaggt caattccctt ttggtccgaa
     5581 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
     5641 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
     5701 agcaattacg ccgcattacc ggaacaccgt ttgaagcact ggtgcggcag aaagtgcctg
     5761 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
     5821 ttgataaaga cggggagtgc attgatgcgt taggcgcgat gggatttgga tcgatcgaga
     5881 tcggtaccgt cacgccacgt ccacagcctg gtaatgacaa gccgcgtctc tttcgtctgg
     5941 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
     6001 tagagaacgt aaaaaaggcc cattatgacg gcgtcttggg tattaatatc ggcaaaaata
     6061 aagatacgcc agtggagcag ggcaaagatg actatctgat ttgtatggaa aaaatctatg
     6121 cctatgcggg atatatcgcc atcaatattt catcgccgaa taccccagga ttacgcacgc
     6181 tgcaatatgg tgaagcgctg gatgatctct taaccgcgat taaaaataag caaaatgatt
     6241 tgcaagcgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
     6301 aagacgaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
     6361 caaccaatac cactctcgat cgttctctgg ttcagggaat gaaaaattgc gatcaaaccg
     6421 gtggcttaag tggtcgtccg cttcagttaa aaagcacaga aattattcgc cgcttgtcac
     6481 tagaattaaa cggtcgctta ccgatcatcg gtgttggcgg catcgactcg gttatcgctg
     6541 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
     6601 aaggtccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt cgaattcctc
     6661 tttataagat aatcagggct ttattttcag acctggttgt tttatattca tcgttgttgc
     6721 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
     6781 gttgctgcgg ttttataggg taggggagag gcagatgcga attaaaccag acgataactg
     6841 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
     6901 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggctt
     6961 ttgtgttgat gatgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
     7021 atctaaagac caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
     7081 accgcaaatg ccgaaaagct ggcattttgt ctcccatggt gaaatgtggg tgcccatgcc
     7141 gggcgatgcg gcctgcgttt ggttgagcga tactcacgag caggtcaatt tgctggtcgt
     7201 tgaatctggc gaaaatgccg cgctatgcct gctggcacaa ccctgcgttg ttattgcggg
     7261 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
     7321 gaatgttgac agcttcagcc tcgaacaggc agtctaacgc gtcagcttaa gggcagtctt
     7381 cggtacacag ctacagcaaa gaatggtgcc atcatcgccc attgctgatt ttttcagcgg
     7441 cgtgacttcg ccttctaaaa gctgaacacg gcaacttcca caaatgcccg cgcggcaaga
     7501 ataagggata cgaattccct gattttctaa ttgctccagc aacacttgtt ggttatttcc
     7561 acgaaatgcc tgtccctgcc agtcaatatc tacatttgcg tccggttgtt gcgtgatgtt
     7621 ggcggtatca tcagcggcag ctgcgccgta aatttttgcc ggagccgttg ccagaatttc
     7681 cacctcatcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
     7741 gacatcgccg ttatcctggg cagtgcggaa agattgcaat gtttttaatg gttcgcctgc
     7801 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggttt
     7861 aaccacatca aacaccacat caccaatgcg aatcactttc cagctatctt cttcccaggc
     7921 tgacgcgccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
     7981 acgttgttgg agatcacgta acgaggcttc gttagcaaga aggtaaggat agccatcagc
     8041 aaatgacaga ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
     8101 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
     8161 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
     8221 agcaaaacga acatatgcgc tactgccatc tggtgcggtg agatgcaagc catcatgtac
     8281 aggtgaagga gtaaaccgta ccatctgggg aaactggcga gcggtaataa acgtaccgtc
     8341 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatcag ccagagcatg
     8401 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
     8461 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
     8521 ctataatgcg caacaatttt cttagcgcct gtcccgttag gctattttat ttgtcagttt
     8581 ggccctgggt tgtgctcgaa atcctcacgt acttgtgtac gctccggttt ctccgcgctc
     8641 tccatgtcca gactgactgc aacaattacg cctactgcgg taggctctta gagtaaaagt
     8701 gacgatatga attctctgtt tgccagtacg gcccgtgggc tggaagagct gttaaaaact
     8761 gaactggaaa atctgggggc cgttgaatgc caggtggttc agggtggggt ccatttcaag
     8821 ggcgacacac ggcttgttta ccagagcctg atgtggagcc gcctggcctc gcgtattatg
     8881 ttgccgctgg gcgagtgtaa ggtttacagc gatttagacc tctatctcgg tgttcaggcg
     8941 atcaactgga cagagatgtt taatcctggc gcgaccttcg ctgtccactt cagtggtttg
     9001 aatgacacca tccgcaacag tcagtacggc gcgatgaaag tgaaagacgc gatcgtcgat
     9061 gctttcacac ggaaaaatct gccgcgtcca aatgttgatc gcgatgcgcc ggatatccgc
     9121 gttaacgtct ggctgcataa agaaaccgcc agtatcgctc ttgatctgag tggtgatggt
     9181 ttacatctgc gtggctatcg cgatcgtgct ggtattgcgc cgatcaaaga aaccctggca
     9241 gccgctattg tgatgcgatc cggctggcag tcaggaacac cgctgctcga tccgatgtgt
     9301 ggttccggta cgttgctgat tgaagcggcg atgctggcga ccgatcgcgc accaggcttg
     9361 caccgtgggc gttggggctt tagcggctgg gcgcagcatg atgaagctat ctggcaggaa
     9421 gtgaaagcgg aagcgcaaac tcgcgcccgt aaaggcctag ctgagtatag ctctcatttt
     9481 tacggttcgg acagcgacgc acgggtgatt caacgtgcgc gcactaacgc ccgtcttgcg
     9541 gggattggtg aactgatcac ctttgaggtg aaagatgtcg cgcaactgac caatccgctg
     9601 ccgaaagggc cgtacggtac agtgttgagc aacccgccat acggtgaacg tctggacagc
     9661 gaaccggcgc tgattgcgct gcatagtctg ctgggccgaa tcatgaaaaa ccagttcggt
     9721 ggctggaatc tctctttgtt tagtgcctcg ccggatctgc taagctgcct gcagctgcgt
     9781 gcagacaaac agtacaaggc gaaaaacggc ccgctggact gcgtacagaa aaattaccac
     9841 gttgccgaaa gcacaccaga cagtaaaccg gcaatggtag cggaagatta cgccaaccgc
     9901 ctgcgtaaga acctcaaaaa attcgagaag tgggcacgcc aggaagggat tgaatgttac
     9961 cgcctgtatg acgccgatct gccggaatat aacgttgccg ttgaccgtta tgccgactgg
    10021 gtggtggtgc aggagtatgc gccgccaaaa actattgatg cgcacaaagc gcgtcagcgt
    10081 ctgttcgata ttatcgctgc aaccatttcg gtactgggga ttgcgccaaa caaactggtg
    10141 ctgaaaaccc gtgaacgcca taagggcaaa aaccagtacc agaaactggg cgagaagggc
    10201 gagtttcttg aagttaccga atataacgct cacttgtggg tgaacctgac ggattatctc
    10261 gatactggtc tgttcctcga tcaccgtatc gcccgccgta tgctcggtca gatgagcaaa
    10321 ggcaaagatt tcctcaacct gttttcttat accggcagcg ctaccgtgca tgcgggatta
    10381 ggcggtgcac gcagcaccac caccgtggat atgtcgcgta cttatctgga atgggcagaa
    10441 cgcaacctgc gtctgaatgg cctgactagg cgtgcgcatc gcctgattca ggccgattgc
    10501 ctggcgtggc tgcgtgaggc aaatgaacag ttcgatctga tctttatcga tccgccaacc
    10561 ttctctaact caaaacgaat ggaagatgcg tttgatgttc agcgcgatca tctggcgctg
    10621 atgaaagatt tgaaacgtct gctgcgtgca ggtgggacga tcatgttctc gaacaacaaa
    10681 cgtggcttcc gtatggatct cgacggcctg gcaaaactgg gactgaaagc acaagaaatt
    10741 acgcaaaaaa cgctctccca ggatttcgcc cgtaaccgcc agatccacaa ctgctggctg
    10801 attaccgcag cctgaaagga atagtaatgt cattaatcag tatgcatggc gcatggctgt
    10861 cgttcagcga cgcgccgctt ctcgataacg cagaactgca tatcgaagat aacgaacgtg
    10921 tttgtctggt gggccgcaac ggcgcaggaa aatcgacgtt aatgaaaatc ctcaaccgtg
    10981 aacaagggct ggatgacggt cgcattattt atgagcaaga tttgattgta gctcgtctgc
    11041 aacaggatcc gccgcgtaac gttgagggta gcgtttatga tttcgttgcc gaaggcattg
    11101 aagaacaagc ggaatatctg aaacgctatc acgatatttc gcgcctggtg atgaacgacc
    11161 cgagcgagaa aaatctcaac gaactggcga aggttcagga acagctggat caccacaacc
    11221 tgtggcagct ggaaaaccgc atcaacgaag tgctggcgca actggggtta gatcctaacg
    11281 ttgcgctgtc gtcgctttcc ggcggctggt tgcgtaaagc ggcattagga cgcgcgctgg
    11341 tgagcaatcc gcgcgtattg ctgctggacg aaccgactaa ccatctggat atcgaaacca
    11401 tcgactggct ggaggggttc cttaaaacct tcaatgggac gattattttc atctcccacg
    11461 accgttcgtt tatccgcaat atggcgacgc gcattgttga tctcgatcgc ggcaagctgg
    11521 tgacctatcc agggaattac gaccagtacc tgcaggaaaa agaagaagcc ctgcgcgtgg
    11581 aagaactaca aaatgccgag ttcgatctca aactggcgca ggaagaagtg tggattcgcc
    11641 aggggatcaa agcacgccgt acccgtaatg aaggccgcgt acgcgccctg aaagcgatgc
    11701 gtcgcgaacg tggtgaacgt cgcgaagtga tgggtaccgc aaagatgcag gtggaagagg
    11761 ccagccgctc cggtaagatc gttttcgaaa tggaagacgt ttgctaccag gttaacggta
    11821 agcaactggt gaaagatttt tctgcccagg ttctacgtgg cgacaaaatt gccctgattg
    11881 gtccgaatgg gtgcggcaaa accacgctgc tgaaactgat gctcggtcag cttcaagcgg
    11941 acagcgggcg tattcacgtt ggcaccaaac tggaagtggc ttatttcgat cagcaccgcg
    12001 cggaactgga tcccgataaa acggtgatgg ataaccttgc cgaaggtaag caagaggtga
    12061 tggttaatgg caagccacgc cacgtattgg gctatttgca ggactttctg ttccatccga
    12121 aacgggcgat gacgccagta cgtgcgtttt ctggcggtga gcggaaccgc ttgctgctgg
    12181 cgcgtttgtt cctcaaacca agcaacttat tgattcttga cgaaccgacc aacgatcttg
    12241 atgtcgaaac gctggaactg ctggaagaac tgatcgacag ctatcagggc acggtattgc
    12301 tggttagcca cgatcgtcag tttgtcgata acaccgttac agaatgctgg atcttcgaag
    12361 gcggcggtaa aattggtcgt tatgttggcg gttatcatga tgcccgtggt cagcaagaac
    12421 aatatgtggc actcaaacag cctgcggtga aaaaaagcga agaagccgcc gcgccaaaag
    12481 cagaaactgt aaaacgcagc agtagcaaac taagctataa attgcagcgc gaactggagc
    12541 agctaccgca attgctcgaa gatctggagg cgaagctgga agccctacag acgcaagtgg
    12601 cggatgcttc cttcttcagt cagccgcatg agcagacgca aaaagtgctt gctgatatgg
    12661 ctgctgcaga gcaggagctg gagcaagcct ttgaacgctg ggagtatctt gaagcgttaa
    12721 aaaatggtgg ctgatcgcaa aatagtcgat ttaaacagag acagctgaca acggtaaaat
    12781 tgcctgatga actgggttta tcaggcatga aaaccaacac tttgttagtc atctgtaaaa
    12841 tagcgcatca ttaaggagta ccaatgtgcg aacatcatca tgccgcgaag cacatcctgt
    12901 gctcgcagtg tgacatgctg gtggcgttac cgcgccttga gcatggtcag aaagcggcat
    12961 gtccccggtg tggcacaacg ttaaccgtgg cgtgggatgc ccctcggcag cgtcccaccg
    13021 cctatgcgtt ggctgcactg ttcatgctgt tgctgtccaa cttgtttcct tttgtgaata
    13081 tgaacgttgc aggagttacc agtgaaatta cattactgga aattcccggc gtgctttttt
    13141 ctgaggacta cgccagcctc ggcacctttt tcctgttgtt tgtgcaactg gttcccgcgt
    13201 tttgtctgat aaccattctg ttactggtga atcgcgcgga attaccggtc cgtttaaaag
    13261 agcaactggc acgggtgctt tttcaactca aaacctgggg aatggcggag attttcctcg
    13321 cgggtgtgct ggtcagtttc gttaaactga tggcttacgg cagcattggg gtaggcagca
    13381 gctttctccc ctggtgttta ttttgtgtcc tgcaactgcg cgcttttcag tgcgttgatc
    13441 gtcgctggtt atgggacgac atcgccccga tgccagaact gcgccagccg ctaaaaccag
    13501 gcgtcacggg gatacgtcag gggctgcgtt cgtgctcctg ttgtacggca atccttcctg
    13561 ctgatgaacc cgtgtgcccg cgttgtagta ccaaagggta cgttcggcgt agaaacagcc
    13621 tgcagtggac actcgcgctg cttgtaacgt ccatcatgct gtatcttccg gctaatattt
    13681 tgcccatcat ggtgacggat ttattaggct cgaagatgcc gtcgacgatt ctcgctgggg
    13741 tcattctgtt atggagcgaa ggctcttatc ccgtcgctgc ggtgatcttt ctggccagta
    13801 ttatggtgcc aacgttaaag atgatcgcca tcgcgtggct gtgttgggat gccaaagggc
    13861 atggcaagcg cgacagtgaa agaatgcatt tgatttatga agttgttgag tttgtaggcc
    13921 gctggtcgat gattgacgtt ttcgttatcg cggtgctctc ggcgctggtg cgtatgggag
    13981 gtttaatgag tatttatccg gcaatgggtg cattaatgtt tgctttagtc gtcataatga
    14041 caatgttttc tgctatgacg tttgacccgc gtttgtcgtg ggatcgtcaa cctgaatcag
    14101 agcatgagga gtcctgacag tatggaatct aataatgggg aagccaaaat ccagaaagtg
    14161 aagaactggt ctcccgtgtg gatatttcct atcgtcacgg cgctcattgg ggcctgggtt
    14221 cttttttatc attacagcca tcagggaccg gaagtgaccc tgatcaccgc gaatgcggaa
    14281 ggaattgaag gtggcaaaac caccattaaa agccgtagcg ttgacgtcgg cgtggttgaa
    14341 agcgccacac tggctgatga tttgacgcac gttgaaatca aagcgcggct gaattccggt
    14401 atggaaaaat tgctgcataa agacaccgtc ttttgggtgg tgaaaccgca gattggtcgc
    14461 gaagggatta gcggcctggg aacgctgctg tctggagttt atatcgaact gcagccaggc
    14521 gcgaaaggca gcaaaatgga taaatacgat ttgctggact caccaccgtt ggccccgcct
    14581 gatgcgaaag gtatccgtgt gattctcgat agcaaaaaag ccgggcagct ctcgccagga
    14641 gatccggtgc tgttccgtgg ctatcgggta ggttcggttg aaaccagcac cttcgatacg
    14701 caaaaacgca atatcagtta tcaactgttc atcaatgcac cttatgaccg actggtgacc
    14761 agcaatgttc gcttctggaa agatagtggc attgcggttg atctgacgtc agcggggatg
    14821 cgtgtggaga tgggctcatt gacaacgctg cttagtggcg gtgttagctt tgatgtgccg
    14881 gaaggtctgg atttagggca gccagtggca ccgaaaacag ctttcgtttt gtatgatgat
    14941 cagaagagca ttcaggattc gttgtacacc gatcacattg attatctgat gttctttaaa
    15001 gattcggtac gcggtctgca accgggagct ccggtagaat tccggggtat tcgcctgggt
    15061 accgtaagca aagtgccatt ctttgcgccg aatatgcgtc agacatttaa cgatgattac
    15121 cgtattccgg tactgattcg tatcgagcca gagcggctga aaatgcagct tggcgaaaat
    15181 gcggatgttg ttgagcacct tggcgaattg ttgaaacgtg gtttacgcgg atcgctgaaa
    15241 accggaaacc tggtcactgg tgcactgtat gttgatctcg atttctatcc aaatacgcct
    15301 gcaataaccg gtattcgtga atttaatggt tatcagatta tcccgactgt tagcggcggc
    15361 ttggcgcaaa tccagcaacg actgatggaa gcgttggata agatcaacaa actgccattg
    15421 aatccgatga ttgaacaggc aaccagtacg ctttctgaaa gtcagcgcac aatgaaaaac
    15481 ctgcaaacga cgctggatag catgaacaag atcctcgcca gccagtcgat gcagcagttg
    15541 ccgacggata tgcagtcaac gttgcgtgaa ttgaatcgca gcatgcaggg cttccagcct
    15601 ggctccgcag cctacaacaa gatggtggcg gatatgcagc gccttgatca ggtgttgcga
    15661 gaactgcaac cggtgctgaa aacgctcaat gagaagagta acgcgctggt atttgaagcg
    15721 aaggacaaaa aagatccaga gccgaagagg gcgaaacaat gaaaaagtgg ctagtgacga
    15781 ttgcagcact gtggctggcc ggatgcagct ccggcgaaat taataaaaac tattaccagt
    15841 tacctgtggt gcagagcggt acacaaagta ccgccagcca gggcaatcgt ctgttatggg
    15901 tagagcaggt cactgttcct gactatctgg cggggaatgg tgtggtttat caaaccagtg
    15961 atgtgaagta tgtgattgcc aacaacaact tgtgggccag cccgttggat caacagttgc
    16021 gcaacaccct ggttgccaac ctgagtacgc aactgcccgg ctgggtggtt gcctcccagc
    16081 ctctgggaag cgcccaggac acgctcaatg ttaccgtaac ggagtttaac ggtcgctatg
    16141 atggcaaggt cattgtcagt ggtgagtggc tgttgaacca ccagggacaa ctgatcaaac
    16201 gtccgttccg tctggaagga gtgcaaactc aggatggtta cgatgagatg gttaaagtgc
    16261 tggccggtgt ctggagtcag gaagccgctt ctattgcaca agagataaag cgtctacctt
    16321 aattataaag atttgtaaat ataaccgtct ccggtatgtt gcctgaggcg gtttttttgt
    16381 ttctaacgtg cggaaaaatt tgttcctctt cacatttttt gtacaaccga catgcccgtg
    16441 tagctcacaa atatgacagt ggcgtgaatt ttgcgcattg acggcagtta tgattcgcgg
    16501 tattgcttaa ctgtgattgc acatttagta atcactgttt tcttttccac cagaaaccag
    16561 tatgagggaa acgaggcatg aagagacaaa aacgagatcg cctggaacgg gcacatcaac
    16621 gtggttatca ggccggcatc gccggacgct caaaagaaat gtgtccctat cagacgctga
    16681 gtcaaaggtc acaatggctg ggaggctggc gagaagccat ggcggacagg gtagtaatgg
    16741 cctgattctg tctctttaaa aagaaacctc cgcattgcgg aggtttcgcc ttttgatact
    16801 ctgtctgatg ataatcagaa ggcagacgta tcctggaaca gaccgacttt caggtcgctg
    16861 gcggtataga tcagacgaca atcaaccagc acttcgccat ctgccagacc cataatcaga
    16921 cgacgattaa caatgcgttt aaagtgaata cggtaggtca cttttttcgc tgtcggcagt
    16981 acctgaccag tgaatttcac ttcgccaacg cccagcgcgc ggcctttacc ttcgccgccc
    17041 agccagccga ggtagaaccc taccagctgc cacattgcgt ccaggcccag gcatcccggc
    17101 ataaccggat cgccaatgaa gtggcatccg aagaaccaca gatccggatt gatatccagt
    17161 tctgcttcaa catacccttt gtcgaagtta ccacccgttt cggtcatttt gaccacacgg
    17221 tctatcatca gcatgttcgg tgctggcaat tgcgggcctt tagcgccaaa cagttcaccg
    17281 tgaccagagg caagaaggtc ttcttttgta taggattcgc gtttatctac catgttctct
    17341 gtaagcctta ttttattgaa gcacgcagga tagctaacac gtgtacgctg aacaagtccg
    17401 atcagttcgg aataaaccag ttcagccaac gtaatggcca tggaaaacgg tgacgtcctt
    17461 cctgttgcga tgcttgcgcg atacgttcct ggatggtttg catcagcgtc gtttggcctt
    17521 cgccatccca caccagattt aataataacg gcagtgcgtc agtcacatcg tctactgccc
    17581 agatggtgaa tttgccttct tctaccgctt tcaccagttc actgtgaaga cttaaatggc
    17641 gaacgttagc agtggggata atgacacctt gtttccccgt taactcacgt tgctggcaaa
    17701 tagcaaagaa gccttcgatt ttctcattta aaccaccgac cggctggacg cggccgaact
    17761 gatcgactga acctgtgata gcgatactct gattcactgg cacatcagcg agggcgctta
    17821 tcagggcgca gagttcagcc atcgaggcac tatcgccatc cacttcactg tatgactgct
    17881 caaatgtcag cgatgctgag aaggggatct gttgctcaag ctgtagttcc gacatcagga
    17941 acgcctgcat gatcatcatc cctttcgcat ggatattgcc gccaagctcc gctttgcgtt
    18001 cgatgtcggt gaattcacca tcgccaatat gcacaacgca gctaatgcga gaaggttcgc
    18061 caaaagcgcg tggatgaccc ggaaattcaa tgaccgaaag ggcgttaatt tgcccgatgc
    18121 gttcgccttc ggtttcaatc aggatttgct cctgaaggat ctcatcctgc atacgttcag
    18181 cgaggaaacc ttcgcgccat tcacgctgct gcagcattaa gtttagctgc tcgccggaga
    18241 aggtgtcgcc atcacacagg gaggcgacct ctttacactg gcggaggatc cactgcgggc
    18301 taagcggaag tgtttcttgt tcaccggtgt agcgtgctgc ttcgcggata agtaccggcc
    18361 aagcatccgc tcccggggca ggtaagtgat tatgtctggc ggtaaatgtc acccagcgac
    18421 accactggct tactgactcc gcatcgacaa tctgcagagt atcttcaaat tcgctataaa
    18481 tagcctgctc tgaaagctct ggctccatct cctggaaatc agccaatgat tcgcgttcgc
    18541 ctaccagaat gactttcagc ttcaatggca ttgaaggcac agagacgggg agagggcgcg
    18601 actcatcaaa cgcaacccag tcaaaacgct cgcggttaac gatatttttc agccgcatcc
    18661 acagcagagg ttgcgccagc agtgtacgca aagagatgat gagaataccg ccatttgcct
    18721 gatgcaccag accaggctgc agggtaatgt cgccattaaa ctggcgcagg cagccaaaga
    18781 gttgctccgc ttctacccag tcggcagcga caacttgcgt taaagtcgca aaattatcat
    18841 ctgcactcac tgcgtgacgt aagcggatgg agtggccgga aacctcatag tgatcgccca
    18901 ccagttggcc tgcatcgctt tgtagcgtac gcgcggcatc ggcaataaga ttcagatact
    18961 cagactcttc cggggcctta gccagcataa aagaggagga tgctcgcgta tgcagcaact
    19021 gctccagcgc aaattgcagt cgcggttgag tatcactgaa taaaggatcg ttttcgtcaa
    19081 tcaaatgtgg ctgagcaaat atttcctgat agctatcggt atcaggaacc aggtcacgcc
    19141 atgcaagttt cgtaatggtc aaagttgatg ttttttagtc tgttgtcaaa gccgcgatta
    19201 taccgtaacc ggcactacag cacacgtaga aagcaccgac aatactcctg gctgggcgtt
    19261 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    19321 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    19381 gttggaaatg gaagtatctg gtgaagaagc atcgcgaagg ggagttaatt acccgttaca
    19441 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctcgaa aatgaacccg
    19501 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    19561 agaccattcg ggccagacgg aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    19621 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgca cagcgtcgcg
    19681 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    19741 acgcgaataa aatgtcttct cttaagcagg atctgcaggc attgctgggt aaggaataac
    19801 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    19861 taccagacga taacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    19921 atctctacgc gacgatccgg agccaagcag tcgatcagtg cagcacgctg tttcacgttg
    19981 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    20041 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    20101 ccctggttgt aagcgtcaga accgatgcgg tcagtgtaac ccagaacaac tacggaaccg
    20161 tctttcggat ctaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    20221 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    20281 gtctgtactt ccggtgccgg agctggagca actaccggag ctgcttcgcc ctgaccgaaa
    20341 cggtaggaaa cacccaggct cagcagaccg ttgtccggac gagtaccgat ggtgtttgcg
    20401 tcaccgatgt tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg
    20461 tactcaacac cacctgcgaa aaccggagaa acgccagtgt cgtggtcttt aaaggatgcg
    20521 ccaccaggta cgttagcctt ggtgtctgca cgccatacca taccacccag acgagtgtag
    20581 atgtccagat cgtcagtgat tgggtaaccc agtttagcgg tcagctgaac gccctgagct
    20641 ttgtatgcgc cgttgatgtt gtcgcctttg tacggcatac gacctaacca gtcgtaaccc
    20701 atttcaaaac caacatacgg gttaacctgg taaccaccaa aagcacctgc acccagttgg
    20761 ttttcgtggg tcggaccatt gttaggaata aaaccagtgt catggtactg ggaccagccc
    20821 agtttagcac cagtgtacca ggtgttatct ttcggagcgg cctgcgctac ggtagcgaaa
    20881 ccagccagtg ccactgcaat cgcgatagct gtctttttca ttttttgcgc ctcgttatca
    20941 tccaaaatac gccatgaata tctccaacga gataacacgg ttaaatcctt caccggggga
    21001 tctgctcaat attaactcta ccgatatctt cggcttatgc cgagcacccc tggcgatgta
    21061 aagtctacaa cgtagttgaa aacttacaag tgtgaactcc gtcaggcata tgaaaaaaaa
    21121 gtcttgtata aggtatgttt aatctttttg tcagcgacaa tttacagaaa aaaaacgcgg
    21181 aaaccgcttc agacaagcct tcgcaaggaa aattagtcac gactgaaagc attggctggg
    21241 cgacaaaaaa agttctagga ttaatcctaa atttacttaa tgatacaaat tagagtgaat
    21301 ttttagcccg gaaagttgtc tcgttgtgtg agaggatgcg cttaccggac gcataataaa
    21361 ccccatagcg ttaccttcat ttgccgcatc aacaagttca gcatgctctt cttcagtcaa
    21421 atcatctgtc aaccaaccga tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat
    21481 tgactccact gtgtggcaag gggagagctg gctaatctgc attactttcg ttaagggtag
    21541 cccagatgcc tgaacccatt cccgactcag tttttgttgc ggtgttaacc agagttgcca
    21601 gcgcgattgc tgaccgagtt gctgtaacaa tggcaacagt agaagttgcg tcatcatggg
    21661 ctgatcttcg cgatagacaa cttcactgat aagcccggct gtagtgtttt ccgtagagac
    21721 acgcgcaatt ttacttgctg cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt
    21781 gtacataatc aatccagccc ctgtgagtta ctgtatggat gtgcagtaca tccagtgaca
    21841 acaaagatca accctatttt cggaaagagc ctcgcaaatt ttgtcgttgg tgacgggaaa
    21901 acataaatta atcttgcccc ttaagaataa gttgcctatt ttcgtagtta acggatccgt
    21961 taatgtgaat cattctttta tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct
    22021 ataagcggat ctataaatca caagaatacc tggcaacgtt gggcacaatt gaataccgat
    22081 cattgtttgg cagttacagc ctgaccgttg acgacacggt gtttgcgatg gtttctgatg
    22141 gtgagttgta tcttcgggct tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg
    22201 tctggctgac atataaaaag tgtggccgat ccgttaccct caattactat cgggttgatg
    22261 aaagtctatg gcgaaatcaa ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag
    22321 cgctgaaaga gaaaagcacg cgcaataccc gggaaagact gaaagatttg cccaatatgt
    22381 cttttcatct ggaagcgatt ctcggggagg tg
//
LOCUS       DAETQL010000063        22317 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.063, whole genome shotgun sequence.
ACCESSION   DAETQL010000063 DAETQL010000000
VERSION     DAETQL010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.063"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1028)
                     /locus_tag="K0W65_002690"
     CDS             complement(<1..1028)
                     /locus_tag="K0W65_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3095896.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1130..2110)
                     /locus_tag="K0W65_002691"
     CDS             complement(1130..2110)
                     /locus_tag="K0W65_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBI3095897.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2118..2828)
                     /locus_tag="K0W65_002692"
     CDS             complement(2118..2828)
                     /locus_tag="K0W65_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HBI3095898.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3045..4868)
                     /gene="typA"
                     /locus_tag="K0W65_002693"
     CDS             complement(3045..4868)
                     /gene="typA"
                     /locus_tag="K0W65_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBI3095899.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5241..6650
                     /gene="glnA"
                     /locus_tag="K0W65_002694"
     CDS             5241..6650
                     /gene="glnA"
                     /locus_tag="K0W65_002694"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBI3095900.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6823..7872
                     /gene="glnL"
                     /locus_tag="K0W65_002695"
     CDS             6823..7872
                     /gene="glnL"
                     /locus_tag="K0W65_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HBI3095901.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7884..9293
                     /gene="glnG"
                     /locus_tag="K0W65_002696"
     CDS             7884..9293
                     /gene="glnG"
                     /locus_tag="K0W65_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBI3095902.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9405..9515
                     /locus_tag="K0W65_002697"
     CDS             9405..9515
                     /locus_tag="K0W65_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBI3095903.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="K0W65_002698"
     CDS             complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="K0W65_002698"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBI3095904.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="K0W65_002699"
     CDS             complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="K0W65_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBI3095905.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12397..13029
                     /locus_tag="K0W65_002700"
     CDS             12397..13029
                     /locus_tag="K0W65_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBI3095906.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13411..16197)
                     /gene="polA"
                     /locus_tag="K0W65_002701"
     CDS             complement(13411..16197)
                     /gene="polA"
                     /locus_tag="K0W65_002701"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBI3095907.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16561..17493
                     /locus_tag="K0W65_002702"
     CDS             16561..17493
                     /locus_tag="K0W65_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBI3095908.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17534..18964)
                     /locus_tag="K0W65_002703"
     CDS             complement(17534..18964)
                     /locus_tag="K0W65_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBI3095909.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="K0W65_002704"
     CDS             complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="K0W65_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBI3095910.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="K0W65_002705"
     CDS             complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="K0W65_002705"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBI3095911.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20825..21094)
                     /locus_tag="K0W65_002706"
     CDS             complement(20825..21094)
                     /locus_tag="K0W65_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBI3095912.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21164..21748
                     /gene="mobA"
                     /locus_tag="K0W65_002707"
     CDS             21164..21748
                     /gene="mobA"
                     /locus_tag="K0W65_002707"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBI3095913.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21730..22257
                     /gene="mobB"
                     /locus_tag="K0W65_002708"
     CDS             21730..22257
                     /gene="mobB"
                     /locus_tag="K0W65_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBI3095914.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5519 a         5597 c         5341 g         5860 t
ORIGIN      
        1 tttgcttcgc cgataggtgc aaaaaatacg gctcgctgtg taaagactat cgcgccaaag
       61 cccagcgtac atgccatccc taaatagacc ggcatacttt cgtgcggcag cataatcaac
      121 agtacgagcg cagcggtact gatgataaag gtgtagcata gatatttact cggcgatttc
      181 aggatcttat ctgaaatcat gccaccaatc ggtccaccaa tcattttcag acagtattgg
      241 ttaatgatgc cgtaagcccc caccagcgca acgggcaata gatagatgtt tttcaggaat
      301 ggaatgaaga atgtcaggcc acagtaaacc gcgtaaacga agaagacgtt gaaagcgata
      361 agccagatgg ttttgtctct cagcactgag gtcatactgg tgttttgcga tgctccgtct
      421 tcttttttca cctcaacgga cggtgcctct tctttgtcat tcaggacaaa gaaaataata
      481 atgcctacgg caatcacaat aagggaatag aaccagatgc ctgctttaaa acctaataag
      541 ccactgccaa accaggtaaa tactgccaac gcagaaaatg ccaccacggt atcgacaatg
      601 ccacgccctg tttcgaagaa gccaaacaac cgaccttgtt gttcgctatt gcccaatcga
      661 cttaccgact tgagcaagac cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc
      721 cagacaaaga ggatgcccca ataccccggc attgtcgtca aataaacacc cagtaatcct
      781 gtcgcaatga gtgagaatga catggtgtat ctgcgcggta gtttatcggc aaaataaata
      841 gacagaaaaa agcccactgt ggtgacaaat gagtttaccg acatagcatt accaatttga
      901 ccattggtca aatggaaata ttcctgcatc gggatataaa acgcatcttt cagcgacggt
      961 aatttataaa ttgtaccgcc acacagtgtt aatagactaa ataacgccca tttctttttc
     1021 gtgagcataa catcccctaa gcgattttta aaatcactgt tttaaaggca ataagatacc
     1081 gaccattcat atggaatggt tttattaact gcgtatgagt taaaacttat tagtggttca
     1141 gaagatggtt tgcctcttgt ttgatttgtt gtagtacgtt acgcacatga tttatctgat
     1201 taattgcatc ttctttttct ttacgtaacc gcgcatccag taaatgattt tctggtaggg
     1261 gtgtttcact catctggcga taagggatcc acggattatc atcttcgtct tcaaagcgga
     1321 acgccgacgc atagtaatca acctcttctt ccaggccata tgccgtcacc tgcggctcat
     1381 catcgccaag gcagataagg tgcgttaata acgctttgaa tggcatatca ccctgacctg
     1441 aaatacaggc tttatgaccc aggccaccct gttctttaac gatcaaggca tctttaatat
     1501 ggacctgggt aatatgcggt gccatcgttt ttaaggcgtc gatgggatgc tcatttgcat
     1561 tgatcatgtt agcaaaatca aacagtaagg aaagagactc catctcactt tctttgacca
     1621 gcgacaccag ctcatgactt tttaaatctt catgctgctc aatcgtaaaa gtcaggccgc
     1681 tgtcctgata cgtttcccgt acataggcaa tgtcgttagc gataatcgat aatacgtcgc
     1741 gcaaattacc ttcataacat ggataaaaac gtacggacgt tgccccagtt ttcaacgcga
     1801 tggcgacggc ttcgtcaata gatgccttat ctgaagcgct ggtttcaata tgaatatcaa
     1861 ggttcagacg gcgggcttta tccccaaagg cagagagttc tttatcgtcc atattaccaa
     1921 gagaaaaacg ctcgccatcc agaacatgga ttttgacgcc acgtagatta ttttcacttg
     1981 cgatatcaag taaatcagca ggcaacactc tttccagccg catatttaaa tgaaaggcat
     2041 aagcatgtaa ataaagagga agagtgtcga cacgttgtag aatctttctt gcgttattta
     2101 ttgtcaccat aaaacaccta cagattaatg atgaggtcta cggcctcaaa tcgccaatat
     2161 tcaatatcta tctggacaat attgccttgc tggtcggcgc gatgcttttc aattaagatc
     2221 gctggcatac ccgcagaacc gccaagatac ttgctgatat cgcccggcat tctgacgggt
     2281 ttgaaaatca atttttttac taccgtctct ttttggtagc acttttccca gactgcagaa
     2341 aatgagtggt tttcaagttg ttcaataaaa cccggagcaa cttcaggatt aatatatgtt
     2401 tcgtgataga aaactttatg tccttccagc gccccccagc cggtgatgcg atagagttca
     2461 gttgatggcg tcactgcaat cagtggcgcg agcgtctcgg gaatatcgct ggtacggttt
     2521 ttctcggtaa acccccaaga aggctctcgc ccttgctcaa ttgcggcccg ctgaaagctc
     2581 gccgacagct ccggactgta attaaatcgc ggctgggtca cgaaccaccc cttacgatcc
     2641 ttacggaaga ttttggattc tgcctcgagg ttcaacaacg cctggcgcag cgtcatacgt
     2701 ttaatgccca gcagttctcc cagctctctt tctgaaggga gttttccacc cggcgtggtg
     2761 atgccatctt tcaaccaccg atccagtttc tcttttgcat tttctacggt ggattgattc
     2821 tccgccattg tttaatcctt ttggtttaaa ccagatgaag catcttacgc ttacgccaaa
     2881 aaagaaagca attaacggtg tatgactttg cagataattg tgacaaagct ctctattgac
     2941 gtaatcacag acttataagg gagatactcg cattggtgtg cgctcacgac aggtaaaaaa
     3001 aaacctgcca tcgctggcag gttttttatg actaaaaaac gaaattaatc gtctttcggt
     3061 gcgcggttgg cgcggcgacg atcgttttcc gtcaggtgac gtttacgaat acggatagag
     3121 gtcggagtca cttctaccag ttcgtcatca tcgatgaact ccagagcttg ttccagagtc
     3181 atgcggatag gcggaaccag aacaacagct tcgtcagtac cggaagcacg catgttggtc
     3241 agtttcttac cggtcaggca gtttacagtc aggtcgttag agcggctatg aataccgata
     3301 atctgacctt cgtaaacttc tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaga
     3361 ccgaacagcg cgaacgcgac agctttaccc tgaccgttag agatcagtac accgttctga
     3421 cgctgaccca cttcgcccgg acgtacgtcg tcgtagtggc tgaaggtgga gtacagcaga
     3481 ccagtaccgg aagtcatggt catgaactca gaacggaagc caatcagacc acggcttggg
     3541 atcacgtagt cgagacgtac gcggccttta ccgtctggat tcatgttttt caggtcgcct
     3601 ttacgttcgc ccagcgcctg catcacagaa ccctgatgct gttcttcaac gtccagcgtc
     3661 acgttttcat acggctcttg tttacgaccg tctatttcac ggaagattac tttcggacgg
     3721 gataccgcca gttcgaaacc ttcacgacgc atgttttcga taagaacaga gaggtgcagt
     3781 tcaccacgac cagaaacgcg gaacgcatcg gcgtcttcgg tttcttctac gcgcagcgca
     3841 acgttgtgta ccagttcttt gttcagacga tcgaggatct gacgagacgt tacgaactta
     3901 ccttctttac cgcagaacgg cgaggtgtta acgcagaaga acatagaaac ggtcggctca
     3961 tcaacggaga gtgccggcag cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg
     4021 ttcagttcgc ccaggcccgt gatcgccacg atatcgccag cttccgccag atcggtttcg
     4081 atacgttcca gaccgaggtg gcccagcact ttaccgactt tcgcgttacg ggttttgcct
     4141 tcgctatcga tgatagtaac ctgctggttc ggcttcactt taccacgctt gatgcggcca
     4201 atgccgataa cgccaacata gctgttgtaa tcgagctgag aaatctgcat ctggaacgga
     4261 ccgtcaaggt caacgtccgg cgcaggaacg tggtcaacaa tcgcctgata cagcggggtc
     4321 atgtcttccg ccatatcttc gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa
     4381 acgatcggga agtccagctg ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc
     4441 tgatccacaa cccaatcagg acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc
     4501 ggcttcaggc cgtaagcaaa cgcttttttg gttacgaagc gcgtttgcgg catcgggccg
     4561 tcaaatgcgt caaccaccag cagcactgag tctaccatgg acattacacg ttcaacttca
     4621 ccaccgaagt cggcgtgccc cggggtatca acgatgttga tacggtaatc attccatttg
     4681 atagcggtgt ttttcgcgag gatggtaatc ccacgctctt tctccaaatc gttggagtcc
     4741 atcacgcgct cttgggtttc ggcacgagag tcgaacgtac cggattgttg gagcagcttg
     4801 tctaccaggg tggttttacc atggtctacg tgcgcgatga tggcgatatt acgcaatttt
     4861 tcgatcacaa ctttgcctca ggcattagaa atagcgcgtt attgtacacg gattaatcgc
     4921 actacaaaac aggatcacaa acatcctccg caaacaagta ttgcagagtc cctttgtgat
     4981 cgctttcacg gagcataaaa agggttatcc aaaggtcatt gcaccaatat ggtgcttaat
     5041 gtttccattg aagcactata ttggtgcaac attcacatca tggtgcagcc cttttgcacg
     5101 atgttaagca tgataacgcc ttttaggggc aatttaaaag ttggcacaga tttcgcttta
     5161 tcttttttac ggcgacacgg ccaaaataat tgcagatttc gttaccacga cgacaatgac
     5221 caatccagga gagttaaagt atgtccgctg aacacgtact gacgatgctg aacgagcacg
     5281 aagtgaagtt tgttgatttg cgcttcaccg ataccaaagg taaagaacaa cacgtcacta
     5341 tccctgctca tcaggtgaat gctgaattct tcgaagaagg caaaatgttt gacggctcct
     5401 cgattggcgg ctggaaaggc attaacgaat ccgacatggt gctgatgcca gacgcatcca
     5461 ctgcagtgat tgacccgttc ttcgccgact ccaccctgat tatccgttgc gacatccttg
     5521 aacctggcac cctgcaaggc tatgaccgtg acccgcgctc tattgcgaag cgcgccgaag
     5581 attacctgcg ttccactggc attgccgaca ccgtactgtt cgggccagaa cctgaattct
     5641 tcctgttcga tgacatccgt ttcggatcat ctatctccgg ttcccacgtt gctatcgacg
     5701 atatcgaagg cgcatggaac tcctccaccc aatacgaagg tggtaacaaa ggtcaccgtc
     5761 cggcagtgaa aggcggttac ttcccggttc cgccggtaga ctctgctcag gatattcgtt
     5821 ctgaaatgtg tctggtgatg gaacagatgg gtctggtggt tgaagcccat caccacgaag
     5881 tagcgactgc tggtcagaac gaagtggcta cccgcttcaa taccatgacc aaaaaagctg
     5941 acgaaattca gatctacaaa tatgtcgtgc ataacgtagc gcaccgcttc ggtaaaaccg
     6001 cgacctttat gccaaaaccg atgttcggtg ataacggctc cggtatgcac tgccacatgt
     6061 ctctgtctaa aaacggcgtt aacctgttcg caggcgacaa atacgcaggt ctgtctgagc
     6121 aggcgctgta ctacattggc ggcgtaatca aacacgctaa agcgattaac gccctggcaa
     6181 acccgaccac caactcttat aagcgtctgg tcccgggcta cgaagcaccg gtaatgctgg
     6241 cttactctgc gcgtaaccgt tctgcgtcta tccgtattcc ggtggtttct tctccgaaag
     6301 cacgtcgtat cgaagtacgt ttcccagatc cggcagctaa cccgtacctg tgctttgctg
     6361 ccctgctgat ggccggtctt gatggtatca agaacaagat ccatccgggc gaagccatgg
     6421 acaaaaacct gtatgacctg ccgccagaag aagcgaaaga gatcccacag gttgcaggct
     6481 ctctggaaga agcactgaac gaactggatc tggaccgcga gttcctgaaa gccggtggcg
     6541 tgttcacgga tgaagcgatc gatgcgtaca tcgctctgcg tcgcgaagaa gatgaccgcg
     6601 tgcgtatgac tccgcatccg gtagagtttg agctgtacta cagcgtctaa tagttgaagt
     6661 tgtactaccc ggcgcacaac gccgggattt agttgccgtg gaaactttca gcccatctct
     6721 gcatgggctt ttttctccgt caattctctg atgcttcgcg ctttttatcc gtaaaaagct
     6781 ataatgcact aaaatggtgc aacctgttca ggagactgct ttatggcaac aggcacgcag
     6841 cccgatgctg ggcagatcct caactcgctg attaacagta ttttgttaat tgatgacaat
     6901 ctggcgatcc attacgccaa ccccgccgcg caacaactgc tcgcccaaag ctcccgcaaa
     6961 ttgtttggta cgccgttacc ggaactgttg agctacttct cattaaatat cgagctgatg
     7021 caagaaagtc tggaggcggg gcaaggcttt accgataacg aagtgacgct ggtcatcgat
     7081 ggtcgctcgc atatcctttc tgtgacggcc cagcgtatgc cggacggcat gatcctgctg
     7141 gagatggcac cgatggataa ccagcgccgc ttaagccagg aacaactaca acatgctcaa
     7201 caagtagctg cccgtgattt agtgcgcggc ctggcacatg agattaaaaa tccgcttggc
     7261 ggtttacgtg gcgcggcgca gttactcagc aaagcgttac ctgacccatc actactcgaa
     7321 tataccaaag tgattatcga acaggcggac cggctgcgaa atttggtcga ccgtctgttg
     7381 gggccgcagc tgcccggtac gcgcgttacc gaaagtattc acaaagtggc tgaacgcgtg
     7441 gtaacgctgg tgtcgatgga actgccggac aacgtgcggt tgattcgtga ttacgatccc
     7501 agcctgccgg aactggcgca cgacccggat caaattgaac aggtcttgct gaatattgtg
     7561 cgcaatgcgc tacaggcgct ggggccggaa ggcggtgaaa tcattctgcg tacccgcacc
     7621 gcgtttcaac tgaccttaca cggcgagcgc taccggctgg cggcgcggat tgatgtggaa
     7681 gataacgggc cgggcattcc gcctcatttg caggatacgc tgttttaccc gatggtcagc
     7741 ggccgcgaag gtggcaccgg gcttggctta tccatcgccc gtaatttgat tgatcagcat
     7801 tcaggcaaaa ttgaatttac cagttggcca ggtcataccg agttctcggt ttacctgcct
     7861 atcaggaaat aaaggtgacg tttatgcaac gagggatagt ctgggtagtc gatgacgata
     7921 gttccatccg ttgggtgctt gaacgtgcgc tcgctggagc gggtttaacc tgtacgacat
     7981 ttgagaacgg cgcggaagta ctggaggcgc tggcgagcaa aacgccggat gtgctgcttt
     8041 cagatatccg tatgccggga atggacgggc tggcgctgct caagcagatt aaacagcgcc
     8101 atccaatgct tccggtcatc attatgaccg cacattccga tctggatgct gccgtcagcg
     8161 cctatcaaca aggggcgttt gattatctgc ccaaaccgtt tgatatcgac gaagcagtgg
     8221 cgctggttga gcgcgctatc agtcattacc aggaacagca gcagccgcgt aatattcagc
     8281 ttaacggccc aacgaccgat atcatcggcg aagcgcaagc catgcaggac gtgttccgta
     8341 ttatcggtcg gctttcgcgt tcttctatta gcgtgctgat taacggcgaa tccggcaccg
     8401 gtaaagaact ggtcgctcat gccctgcatc gccacagtcc gcgcaccaaa gcgccgttta
     8461 tcgcgctgaa tatggcagct atcccaaaag atttgatcga atcagaactg tttggccacg
     8521 agaaaggcgc gtttactggc gcgaatacca ttcgtcaggg gcgttttgaa caggccgatg
     8581 gcggcacgtt atttctcgac gaaattggtg atatgccgct ggatgtgcag acgcgtttgc
     8641 tgcgcgtgct ggcagacggt cagttttatc gcgttggcgg ctatgcgccg gtgaaagtgg
     8701 atgtgcggat tatcgctgcc actcaccaga atcttgaaca gcgggtgcag gaaggtaagt
     8761 tccgtgagga tctgttccac cgcctgaacg ttatccgcgt tcatctgccg ccgctgcgcg
     8821 aacgtcggga agatattccc cgtctggcgc gccatttttt acaggttgcc gcgcgcgaac
     8881 tgggcgtaga agcgaagttg ctgcatccgg aaaccgaagc tgctctgacg cgtctggcgt
     8941 ggccaggcaa cgtgcgccag ctggaaaaca cctgccgctg gctaacggtg atggccgccg
     9001 ggcaggaagt gttgattcag gatttgcccg gcgaactgtt tgaatcaacg gttgcggaga
     9061 gtacttcgca aatgcaaccg gacagttggg cgacactttt agcgcagtgg gcagacagag
     9121 cgctgcgttc cggtcatcaa aatctgcttt ccgaagcgca gccagagctg gagcggacgt
     9181 tactgacgac cgcgttgcga catacgcagg ggcataaaca ggaagcggcg cggctactcg
     9241 gctggggccg caacactctg acgcgtaagt taaaagagct ggggatggag tgattcacgg
     9301 cttgtgtgta aagattgatt attgagcgca aattgctggt attttgcgct ttactgttcc
     9361 gataagttca gtatgatctt gcccggaaaa cggggagagt cattatgctg gaatcaataa
     9421 ttaatctggt atcgagtggc gcagttgaca gccacacacc gcaaactgcc gttgctgccg
     9481 tgctgtgtgc cgcgatgatt gggctgttta gctgaagctc gttgtttatg ccggatgcgg
     9541 agaaagtcgt aggcctgata agacgcgacc agcgtcgcat caggcatcat gctcagatgc
     9601 ctgatgcggc gtaaacgcct tatccggcct acaaaagcaa taatttcaac aggttttgtt
     9661 gcccagcgca tcaggcaatc ttgttctcag cacattgatg gcgctttcgt tttctacttt
     9721 gtaaacgaag cgccattcac tctttaaatc acccgtgaga actgctgcat ccgcgctttc
     9781 tggcgcagat aggtatcaaa gcacatgcaa atgttgcgga tcagcaagcg acctttcgcc
     9841 gtcacctgta ttcccttctc atccacatcc accagcccat cttttgctaa cggggcgagc
     9901 agcttgagat cttccgcaaa gtaatcagcg aagtgcaaat cccactgttt ctcaagaggg
     9961 gcgtaatcca gacggaagtt gcagatgagc gacttaatca catcgcggcg aatacagtca
    10021 tcacgcgtta gcgcaatacc acgccacagc gcattgcctt gttcatacac ttgctgatag
    10081 tactgcttca actctttctg gttctgcgcg tagcagtcgc caatcatgct gatggcggaa
    10141 acgcccatcc ccagcagatc ggtatcgccc tgagtggtgt agccctggaa gttacgatgc
    10201 agcacgcctt cacgctgggc caccgccagc tcgtcatcag gacgggcaaa gtgatccata
    10261 ccgataaact gatagcccga ttgcgtcagg aaggcgatgg tttcctgcag gatatcgagt
    10321 ttttgctgcg gactcggcag gtcagcatct ttgattttgc gctgagcagc aaaaatggtc
    10381 ggcagatgcg cgtagttaaa aacgctcaga cgatcggggt tcagctccgc cacacgtttc
    10441 agggtaaagg cgaaactctc cggcgtctgt ttcggcaggc cgtaaatcag gtcgatgttg
    10501 gtggaggtaa aaccaatctc acgcgcatgg ttaagcagtg caaagatgaa ctcttcatcc
    10561 tgctcgcggt taaccagacg ttgcacttct ttgttgaagt cctgcacgcc catgctcagg
    10621 cgattaaagc cttcggcgcg taaatgatcg agtacatcca gttcgatttc ccgcggatcg
    10681 acttcgatcg aaatctccgc atcggtattg aaatggaagt tttcgcgcag cagcttcatc
    10741 agacggctga tttgcgcttt attcagatac gtcggcgttc cgccgcccca gtgcaattgg
    10801 ctgacgtgac gcccggcgaa cagcggtgca cgatggacga tttcttgctc cagcgcgtcc
    10861 agatactgat cggccttgtg ctgctggcga gtaacaatct tattgcaacc gcagaagtaa
    10921 caaagcttat ggcagaacgg gatatgtacg tagagagata atggacgctc aggatagcgc
    10981 gccacggctt gtaaaaacgc ctgttcgccg aagtcttctg aaaactccag cgcggtcggg
    11041 tacgaggtgt atcgtggccc ggaatagtta tatttctgga tcagggccag atcccagtcg
    11101 atttgctgta cagacatgct cactccttcc gatggcgtct ctggcggcta cggcgtaccg
    11161 gcggatgccc gtttcgggtc atcagccggt tgcgcagcca cttttgtcgc cgcgacaacc
    11221 gctgtagttt aacgaataac cacaccagat aacatataac cggaagggtt atcagcagaa
    11281 cgggaaggcc cacggcggca atccgttagt tgccccgcag cagacgcatc atgtcttctt
    11341 gcttctcgtc ttcttcctct tcttcgtcat cgtcataaga gaggccgagt ttctgcatca
    11401 actcatcaat acgatccagt ttagcatcca cccaggattg ctcttcggcg ctcagggttt
    11461 cgcctgcttc cagacgttcc agcagcgcat ccagacgctc atccgtttcc agtaattcca
    11521 actccgcctg cggtgaaagc ataggtttct cactcttcgg tttgtgctgt ttggtgactt
    11581 tttcagtcac tcccaatgga ataggggttt tactgccaat acgtggatct tttggtgcgt
    11641 tctggccttt gctgcctgac gtggtgtttc cgcccgctgc gcggctgccc ggcgcatgac
    11701 cacgacgttt tttctgacgc ttacggtcac gagcttcctg atccagctcc tcgcgtgttt
    11761 ttcgacgcgc ttttgcatga cctttgctgc gtgagtttga agatgatggt ttcataatgg
    11821 ttcgtctttt agccgtttta ttcagtatag atttgcggcg gaatctaaca gaaagcaagc
    11881 aaagaaaaaa ggcgacagat tactctgtcg ccttttttcc tgactcataa cccttaacgg
    11941 gtcttacaat ccttgcaggc cgccaacaca ccctgttttt ccattagccg gaacaatccg
    12001 tgttgattcc atttccgttt aattacgtct ccggacgttt gtcttcctga caatcctctg
    12061 tcttcgcctc ctggcgctcc tgaccctcat cctgagtcat tgttcctgtt agcgtcctcg
    12121 ctctatgggt gctactttac gcctttgctt taaacaaaca atcaaccgcc atcagtattt
    12181 gcacattttc agagttttat ataaaagcaa ttcactgatt tatatagctt atttgacatg
    12241 catcgcagaa ttttctctgc tatttcgcat aaagactatc ggcaatgtct cacaaagcgt
    12301 atggctttta cttacaacgc tcacgccgaa aaatccgcct tttattatgg ttcaggtata
    12361 atcgccacaa agctttgact gacggagacg accgctttga ctaatttgaa ttatcaacag
    12421 acgcattttg tgatgagtgc gcctgatatt cgccacctac cttccgatac cggaattgaa
    12481 gtggcttttg caggccgttc caacgcaggt aaatccagcg cgctgaacac gctgactaac
    12541 cagaaaagcc tggctcgtac ctcaaaaacc ccagggcgca cccagcttat caacctgttt
    12601 gaagtggctg acggcaagcg tctggttgac ttgcctgggt acggttatgc ggaagtcccg
    12661 gaagagatga agcgcaaatg gcagcgtgcg ctcggcgaat acctcgaaaa acgtcagagc
    12721 ctgcaaggtc tggtggtgct aatggatatt cgccatccgc tgaaagattt ggatcagcag
    12781 atgattgagt gggcggtaga cagcaatatc gccgttctgg tgctgctgac caaagcggac
    12841 aaactggcaa gcggcgcacg taaagcgcta ttgaatatgg tgcgtgaagc tgtactggcg
    12901 tttaacggtg atgtgcaggt tgaaacgttt tcttcgttga agaaacaagg cgtagacaag
    12961 ctgaggcaga aactggatac ctggtttagc gagatgcagc ctgtagaaga aacgcaggac
    13021 ggcgaataat tttcttgcct taatgcttgt gccggatgtg gcgtatccgg cccgtaaatt
    13081 caatagatta caattctacc gttggcctga taaacatagc gcatcaggca ttacggatct
    13141 tttctttcgc ccaataaaaa acgccccagt cattactgac tggggcggct aaaatattca
    13201 gccaaatccg attacgtgaa gtaaaaggtc tgaaagatag aacatcttac ctctgtaccc
    13261 tacgcgacta actttactct tttttgttca gttcagaaag cactttttgt aatttttttt
    13321 cagttgttgc ataggaattt ctaatagcca tcacaaaacg tgacagctta tgttgcttac
    13381 ttacgaaaaa agggcatgtt caggcgaatc ttagtgcgcc tgatcccagt tttcgccact
    13441 ccccacttcc accagcaacg gcacatccag acgggtacag ttttccatca gttgatgaat
    13501 ctgcttcgcg acggcatcaa catcatcttt atgaacttca aataccagtt catcgtgtac
    13561 ctgcatgatc atacgtacac gcggttgctc agcctgtaac cacgcatcaa cggcaatcat
    13621 cgcccgtttg ataatgtcgg cggcggttcc ctgcattggc gcgttaatgg ctgcacgttc
    13681 agccgctgca cgacgagcac cattgctgga tttgatatcc ggcagataca gacggcgtcc
    13741 gtccagcgtt tcaacgtagc ccagctcttt cgcctgagca cgggtgcgtt ccatatactc
    13801 cagcacgcca gggtagcgtt cgaagtaaag gtccatgtac ttctgcgctt ctttacgtgg
    13861 aatgttcaat tgccgcgcca gaccgaaagc actcatgcca taaatcagac caaagttgat
    13921 cgctttcgcg ctacggcgtt gctcgctggt gacggtttcc agtggcaaac caaacacttc
    13981 tgccgccgtt gcccggtgga tatcttttcc ttccgcgaat gcggtcagca agcctttatc
    14041 acgcgagaga tgcgccataa tgcgcagttc aatctgcgag tagtccgctg agacaatcac
    14101 ataatcctct ggcgcaataa acgcctggcg gatacgacga ccttcttcgt tacgcaccgg
    14161 aatgttttgc aggttaggat cggttgacga taaacgtccc gttgcagtta ctgcctggtg
    14221 ataagaggta tgcacacgcc cggttttcgg gttgatcatc agcggcagct tgtcggtgta
    14281 agtcgacttc agcttcgcca gaccacgata ctccagaatc acttttggca acggatagtc
    14341 cagcgccagt tcttccagta cctcttccga cgttgacggc gcgccacccg gcgttttctt
    14401 cagcggttta ataccctgtt tttcaaacag aatggtttgc aactgcttgg tggaagaaag
    14461 gttaaattct tcacctgcaa tttcatgcgc tttcttttcc agctcagcca gacgcaaggt
    14521 gagctcttca gaatgattgt gcagcacttt cggatcgatc ttcacaccgt tacgttcaat
    14581 gcgtgaaagc accggcacca gcggcatttc gatattctcg aagacgttca acggcccttt
    14641 gtgtttctgc agatccggcc acattttcag atgcaactgc aaggtgacat ctgcatcttc
    14701 ggcggcgtaa cgtccggcct cttcgagggc aatctggtta aaggtcagtt gatttttgcc
    14761 tttacccgca atctcttcaa aagtgatggt tttgtgcttc aaccaacgtt ccgcgaggct
    14821 gtccatatcg tgacgcccgg caacgctatt gagaatgtag gactccagca tggtatcaaa
    14881 cgcaatccca cgcagttcaa tgccgtaatt cgccagaata ccgcgatcgt atttcaggtt
    14941 ttgcccgacc ttcagcgcct tttcatcttc cagcagcggt tttagcaact cgagtgcacg
    15001 ctcgtgagag atttgatcgg gcgcatcaag ataatcatga gcaaccggaa tatatgccgc
    15061 tacgcctggc tcgatagcaa aagaaagccc gaccaggtta gcagagatgt tatcaaggct
    15121 gtcggtttcg gtatcaaatg caaataccgg cgctttttcc agcttcgcaa tccacgcttt
    15181 cagtgtttct tcatcaagga tagtgacgta gttgtcataa gaaatcaccg ttgccgtcac
    15241 ttctggcgct tcgtctgcaa cactggtttc ctgcggcttc gcggctgttt ttgccccttt
    15301 ggcctgtaac catttgccca cttcgacatc agcagtccag cgtttgaact catacttttt
    15361 gaacagcccc aacaactctt ccgctgccgg ttgctgcact tccagttgtt cacaggtcag
    15421 ctccagttca acatcggttt taatcgtcgc caactggtat gagagataag caacttcttt
    15481 gttttgctcg agcttcgctg ccattgtttt cgcgccacgg aagctcaacc cagcaatttt
    15541 ttctggctcg gcatacagcg tatccagccc gccaagacct tgcagcaatg cctgcgcggt
    15601 tttttcaccg acgcccggta cgccaggaat gttatcagag gagtcaccca tcagcgccag
    15661 gaagtcgatg atcaattctg gcggcacgcc gtacttattc accacctctt ccggtccgag
    15721 gatggtattc gtcatggtat tgataagcgt aatatttggc gtcaccagct gcgccatatc
    15781 tttatcgcca gtgctaatca gcaccggacg cccggctttt tcggcttcgc gcgccagagt
    15841 accgataacg tcgtccgctt ctacgccaga aaccgccagc agcggcagtc ccatcgcttt
    15901 aaccatcgcg tgcagaggtt cgatttgtgc tcgcagatcg tccggcattg gcgggcgatg
    15961 tgatttgtaa tgttcaaaca gttcatcacg aaaggttttt cccttggcgt caaagaccac
    16021 cgctgcatgc gtcggtttat attgcatgat cagactgcgc agcatgttga ggacaccata
    16081 catcgcaccg gtcggctcgc ctgcgctgtt agtcagcggg ggaaacgcgt gatatgcgcg
    16141 ataaagataa gatgaaccat ctacaaggat aagtggattt tgggggatct gaaccataat
    16201 gtccgtgcct gtttatcaga ttatgggtaa aggatgccac agacaggatg aaaacatgag
    16261 tttttcaccg cttttcgtga aaatcttttg tagatctgct ggatcgctcg caaatcgtca
    16321 tgtggataac tttgtgcata aattatttac atcgctttta atttattatc atgcttttta
    16381 cagcatgatg tttgtctttg tttttcatgc agttaaaaat aaatccatcc ctaataattg
    16441 agataatggg attttttcta atgtggatag atatgaatta tttttctcct taaggatcat
    16501 ccgttatttg ggtcattttc tgctaaaatc tgcgccttgc gcttctttag tgcactgtct
    16561 atggctaacc ttttgaataa attcattatg acgagaatac tcgctgcgat aacccttttg
    16621 ctgagtatcg tattgaccat tttggtcact attttttgtt ccgtcccgat catcattgcc
    16681 gggattgtaa aactattgct acctgtgcca gtcatttggc gaaaggtttc gcgtttttgt
    16741 gattttatga tgtattgctg gtgtgaaggt ctggcggtat tactgcatct gaacccacac
    16801 ctgcaatggg aagttcacgg gctggaaggg ttaagtaaga agaactggta tctgcttatt
    16861 tgtaaccacc gtagttgggc agatattgtc gtactgtgcg tgctgtttcg taagcacatt
    16921 ccgatgaata aatatttcct caagcaacag ctggcctggg tgccatttct tggcctggcg
    16981 tgctgggcgc tggatatgcc gtttatgaag cgttattccc gcgcttattt attacgtcat
    17041 ccggagcgac gtggtcaaga tgttgaaacc actcggcgtt cttgtgaaaa gtttcgcctg
    17101 catcccacca ccattgtcaa tttcgttgaa ggctcccgtt tcacgcaaga aaaacatcag
    17161 caaacccact ccacttttca aaacttgttg ccaccaaagg cggcaggcat tgcgatggcg
    17221 ctaaatgtac tgggtaaaca attcgataaa ctgcttaatg ttacgctgtg ttatccggac
    17281 aataaccgtc agccgttctt cgatatgtta agcggtaaac tgacgcggat tgtcgtacat
    17341 gtggatttac agcctattgc cgatgagtta catggcgact acatcaatga caaaagcttt
    17401 aagcgccatt ttcagcaatg gctgaactca ttgtggcagg aaaaagaccg gcttctgacg
    17461 tctctcatgt catcacagcg tcagaataag taagttttac tcgcctctga atgagcagag
    17521 gcgagtgagt attttaatga acaaaacgtc cggcacgaga cataaattct tcttcgctca
    17581 tctcctgtcc gttaaaaaca actttacccg gtgtataacg cagctgcagc gaggcggtat
    17641 tgttgtcaat cgtgattaac tgaaacatct gacctaatgt catcatcccg ctgatttgtt
    17701 tatcagcctg cttttgcgct ttttccgcat ccattccttc agataaatta agctgttttg
    17761 cggtttctgt gacgacatta agcggtaact tcacatcaaa attgagcgat ttgatatctt
    17821 tgtttgtgga tgatgaagat ttggctgggt cggcaatact gatatccaga ttggcattta
    17881 gttcgccgag tgcgttcttc catcttaccg gttgtttaat ggtcggctca cttttttgta
    17941 ataacggtaa atactctttg aacaatgcag cattcacttc ttgcagagca acttcatcgt
    18001 ttgctaactc agggtgtgca gcaagctgct tctgcatcgc aatgttatac tggatgataa
    18061 attggcgcac tgctgaggga tcaatagatt cagcaatcaa tgaccactca ccactaccga
    18121 gttgctgatt cccttttgtt aacttatcaa gttcgtaggt cagattgaca ttgatataat
    18181 ctttgtcttt aacgcgatcc agcctggttt ttgcaacgaa agcatcgatt tgggcaacgt
    18241 cttccccgtg attgataatg ttaatctgat taaatttcga ctcgctttca ccaaccggaa
    18301 atttcttttc ttctaatcgc gccagagaat caatggtaaa actttttgcc gttactttgt
    18361 tcattggtga taattgcaga acaatatctt cgactttgcc ttcaatatcc aggttagaca
    18421 atgaactacc atcaccatta aaagtaaact ggccctcacc aaaagaaaat tttccatact
    18481 ctgcaggggc aacatctaat gtcgtagaga atgagccacc aaacgcgaat cgcaatgtcg
    18541 cttccacggg cgatttattt ttcgcggcga taaatagcgg ttgtgtcagt tcattattca
    18601 ctaagttcac ttttgccgct gccagtgctg ggatgacatt accatgcatt aacattgtga
    18661 tgggcaacgg accgtgctca acatccacat caaatacaac tttctggcct ggcttgatat
    18721 tgagatcggg tgcaccgtta tcgaatgtca tttgcatttg aaaacgggaa ttaaaaaagc
    18781 ctttgtcaaa gtttttataa cttaacgtca tatcgtaggc atgttcacct ttctttttcg
    18841 catcgttaaa atctttaata aacttttcga taccaggctg aatttgcgta cctgtgtacc
    18901 atgtgccacc accccagatt acggctaacg caacaataac acctgtagct gacttacgta
    18961 tcatataaat attccctgat aaaacagcaa aactttgaat atccacttat gctgaaataa
    19021 tgtccagcgg caggatgcat tatcagaatg tatttatatt tattaaataa taaaaaaagc
    19081 ccgtgaatat tcacgggctt tatgtaattt acattgaatt atttttcctc ggacagatat
    19141 ttcaccgtat cagcatactg ctgaacaaaa acatccatat tgctggtatc cataccctgc
    19201 ggattcagct gatatttacc gttaacaaac atcgccggaa caccacgcaa ttgcacatca
    19261 gctgcggctt tttcctgctg agcgaccaga gatttcacca cgaagctgtt ccacgccgcg
    19321 tcgtactctt cacctttaat acctgcattg ataaatacat cgcggatatc agatgctgaa
    19381 cgaatggtct gggttttctg tacgccttca aacagcggaa cagtcacttt gtcttccacg
    19441 cccagcgcca tcgccacagc ccatgcctga gtcagatctt tgcccaggtc accacccatg
    19501 aagttgacgt ggtatttagt catcttcacg ccttccggca gttttttctt cacattatca
    19561 gaaatatgca gaacttcttc aaactgatag cagtgcgggc agaagaaaga gaaaaactcc
    19621 agcacttgcg gcgcgccagc taccggtttt tccagggtag tgtactgttt accatcttca
    19681 tactgcgccg ccgatgcgct aaacgctaaa actaaaccag ccagcgccag ccaaatcttt
    19741 ttcatgatct actctctccg attaatacat aggtgttaat tgcaaagggg gttcttgtag
    19801 aacttttgcc tgttctataa aagtcgccgt ctgtcgtagc cagtaatctt ccccggttaa
    19861 ccacgggaaa tttttcggga acgcgggatc agcccaatgc cgcattagcc aggcaagata
    19921 ataaaccaaa cgcatggcgc gtaaaggttc aatcagtccg atttcagcgg tgtcgaactc
    19981 gctaaattct tcataagctt caataatagt ttccagttgc atccgctgct gggctttatc
    20041 gccattgagc aacatccaca aatcctgaat agctggacca ttacgtgcat catccagatc
    20101 aacaaacatt ggaccatcgc gccagagaat attcccggcg tggcagtctc catgtagccg
    20161 cagaacggtg aaatcttccc gccagtgtgc tgtaacggcg gcgatcagct catctgtcgc
    20221 tttcagaaat gccgctttca acccggaagg tatcagtgta gcgtcctcaa acagcttgcg
    20281 aggctcaatg agatattcat tcagaccgat ggtcgggcga tgaataaaaa gctgtttgcg
    20341 ccccgtctgg tgcatacgcc ctaaataacg cccaaccgct tccatctgat cgatattatc
    20401 agcttcgaac tggcgaccac cgacgcttgg aaaaacagcg aaataaaatc cctgatgatt
    20461 caataaagtc tgaccgttaa aggccacagg tgctgcgacc ggaacttcat catttaccag
    20521 ctgcaacgca aattgatgtt cttcgaggat ttgatcggct gtccaacgtt cagggcgata
    20581 aaatttgacg acaaaacgtc gacgctcttc gtcctgaaat tgatagacac ggttttcata
    20641 gctgttaagc ggggtaagac cggaatccac ccggatccct tgcttaaaca gagcgtccat
    20701 gatggtatcc gggtgtagtg tctggaaagt aaaagcgctg ttgttcatcc catcatccgg
    20761 aaaacattac gaatgatgaa ggatatcatt tcacgatgat tttggtggcg ccgcttacaa
    20821 gcttttactc tttaattacg ccgcgagcgc gtaacagcgc cgttttgaag tcttcttcat
    20881 aatctttctg caaacccggg atcaccgcat ctttcgcgga atcacgcatt ttcaggtgat
    20941 agatcagaat gtcatccgtc aaatccgcca gttcgccgtc aaaacctgac tctttcgcca
    21001 gtttctgcaa aaattgcaac aagttaaagt ctggctcttt ttgccaggct ggctggagga
    21061 gttcaataac ttcattcaga cgtttacatt tcatagtaat gctccttact cttgagacag
    21121 acacgttagc agggtcaatc ccacaataaa agaggcgata tcggtgaatc taatgacgac
    21181 gataacaggc gttgtgctgg caggcggtaa agccagacga atgggcggcg tagataaagg
    21241 attgctggac ttaaatggca aaccgctgtg gcaacatgtc gctgacgcgc ttaagacgca
    21301 gctctctcac gtcgtggtta atgctaatcg tcatcaggaa atctatcagg caggcggtct
    21361 gaaagtgatt gaagattcac tggcggatta cccaggccct ctggcaggaa tgctttcagt
    21421 aatgcagcag gaagctggtg agtggttttt gttttgtccg tgcgatacgc cttacattcc
    21481 ccatgattta gccgcccggc ttaatcatca gcgcaaagat gcgcctgtcg tgtgggtcca
    21541 tgacggtgaa cgcgatcacc cgactattgc tctggtaaac cgcgctattg agcctttatt
    21601 actggaatat ctgcaagcag gagaacgccg ggtaatggca tttatgcgtc tggctggcgg
    21661 tcatgcggtt gatttcagcg atcataaaga tgcatttgtt aacgtgaata cgccagagga
    21721 gcttgcccga tggcaggaaa aacgatgata ccgttactcg cctttgccgc gtggagtggt
    21781 accggaaaaa ctacgctttt gaaaaaactg atcccggcat tatgcgccag agggatccgt
    21841 ccggggctga ttaagcatac gcaccatgat atggatgttg ataagccagg caaagatagc
    21901 tatgagctgc gcaaggctgg cgcggtacaa accatcgttg ccagccagca acgatgggcc
    21961 ttgatgacgg aaacaccaga cgaagaagag ctggatctac attttctcgc aagtcgaatg
    22021 gatacctcaa agctggattt gattctggtc gaagggttta agcatgaaga gatcgcaaag
    22081 attgtgctgt ttcgcgatgg agccggacat cgacctgaag aattagtgat agacaggcat
    22141 gttattgctg tagccagtga tgtgccgctt aatcttgatg tcgcattact ggatattaat
    22201 gatgttgagg ggctggctga ttttgtggtg gagtggatgc aaaagcagaa cggataagca
    22261 agagtaggct ggatcagacg cagccagcgt cgcacccggc atttttttct gcgccaa
//
LOCUS       DAETQL010000064        21617 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.064, whole genome shotgun sequence.
ACCESSION   DAETQL010000064 DAETQL010000000
VERSION     DAETQL010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.064"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="K0W65_002709"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="K0W65_002709"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBI3095915.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="K0W65_002710"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="K0W65_002710"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBI3095916.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="K0W65_002711"
     CDS             complement(3121..3420)
                     /locus_tag="K0W65_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HBI3095917.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="K0W65_002712"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="K0W65_002712"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HBI3095918.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="K0W65_002713"
     CDS             complement(4632..5069)
                     /locus_tag="K0W65_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HBI3095919.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="K0W65_002714"
     CDS             complement(5115..5456)
                     /locus_tag="K0W65_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095920.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="K0W65_002715"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="K0W65_002715"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBI3095921.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="K0W65_002716"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="K0W65_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="K0W65_002717"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="K0W65_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3095922.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="K0W65_002718"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="K0W65_002718"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HBI3095923.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="K0W65_002719"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="K0W65_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HBI3095924.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13217
                     /locus_tag="K0W65_002720"
     CDS             12687..13217
                     /locus_tag="K0W65_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBI3095925.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13329..13865
                     /locus_tag="K0W65_002721"
     CDS             13329..13865
                     /locus_tag="K0W65_002721"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBI3095926.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13973..14797)
                     /locus_tag="K0W65_002722"
     CDS             complement(13973..14797)
                     /locus_tag="K0W65_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HBI3095927.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17146
                     /gene="malS"
                     /locus_tag="K0W65_002723"
                     /pseudo
     CDS             15117..17146
                     /gene="malS"
                     /locus_tag="K0W65_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17324..18577
                     /gene="avtA"
                     /locus_tag="K0W65_002724"
     CDS             17324..18577
                     /gene="avtA"
                     /locus_tag="K0W65_002724"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HBI3095928.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18728..19201)
                     /locus_tag="K0W65_002725"
     CDS             complement(18728..19201)
                     /locus_tag="K0W65_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBI3095929.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="K0W65_002726"
     CDS             complement(19303..20151)
                     /gene="yiaJ"
                     /locus_tag="K0W65_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HBI3095930.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20351..21343
                     /gene="yiaK"
                     /locus_tag="K0W65_002727"
     CDS             20351..21343
                     /gene="yiaK"
                     /locus_tag="K0W65_002727"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HBI3095931.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILP
                     MGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRD
                     AKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21355..21501
                     /locus_tag="K0W65_002728"
                     /pseudo
     CDS             21355..21501
                     /locus_tag="K0W65_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
     gene            21558..>21617
                     /locus_tag="K0W65_002729"
     CDS             21558..>21617
                     /locus_tag="K0W65_002729"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095932.1"
                     /translation="MASISIRCPSCSATEGVVRN"
BASE COUNT         5425 a         5514 c         5306 g         5372 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcgccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaaatggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgagcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcacccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgcccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgcgccga accggtagca aacctggtcc gtgaatgccc
    15481 gaaatgggat ggattaccgc tcacgctgga tgtcagcgcc actttcccgg aaggagccgc
    15541 cgtacgggat tattacagcc aacaaattgc gatagtgaag aacggtcaaa taacgttaca
    15601 acccgctgct accagcaacg gtttactcct gctggaacgg gcagaaactg acacatccgc
    15661 ccctttcgac tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg
    15721 cgatcccagt aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac
    15781 ttttcacggc ggcgatttac gcggcctgac caacaaactg gattacctcc agcagttggg
    15841 cgttaatgct ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggtgg
    15901 tacaaaaggc gatttcccgc attatgccta ccacggttat tacacacagg actggacgaa
    15961 tcttgatgcc aatatgggca acgaagccga tctacggacg ctggttgata gcgcacatca
    16021 gcgcggtatt cgtattctct ttgatgtcgt gatgaaccac accagctatg ccacgctggc
    16081 ggatatgcag gagtatcagt ttggcgcgtt atatctttct ggtgacgaag tgaaaaaaac
    16141 gctgggtgaa cgctggagcg actggaaacc tgccgccgga caaacctggc atagctttaa
    16201 cgattacatt aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat
    16261 cagaacggat atcggcgatt acgacaatcc tggattcgac gatctcacta tgtcgctggc
    16321 ctttttgccg gatatcaaaa ccgaatcaac taccgcttct ggtctgccgg tgttctataa
    16381 aaacaaaacg gatactcacg ctaaagtcat cgaaggcttt acacctcgcg attacttaac
    16441 ccactggtta agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc
    16501 caaacatgtt gagttgcccg cctggcagca actgaaaacc gaagccagct ccgcgcttcg
    16561 cgaatggaaa aaaactaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg
    16621 tgaagcctgg ggccacggcg tgatgcaaag tgactactat cgccacggct tcgatgcgat
    16681 gatcaatttc gattatcagg agcaggcggc gaaagcagtc gattgtctgg cgcagatgga
    16741 tacgacctgg cagcaaatgg cggagaaatt gcagggtttc aacgtgttga gctacctctc
    16801 gtcgcatgat acccgtctgt tccgtgaagg gggcgacaaa gcagcagagt tattactatt
    16861 agcgccaggc gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac
    16921 aggttctgat ccgctgcaag gtacacgttc ggatatgaac tggcaggatg ttagcggtaa
    16981 atctgccgcc aacgtcgcgc actggcagaa aatcagccag ttccgcgccc gccatcccgc
    17041 aattggcgcg ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga
    17101 gcatggcgac gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc
    17161 cgttcgtcag tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcgtt
    17221 gcgggataaa gcaccgctca ctcttcaaca gaccgatttg caccccagca aatgtagcgt
    17281 tattgttacc ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt
    17341 tggtgacaaa tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg
    17401 tttacgcacg cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat
    17461 gcaggactac ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc
    17521 actgtgtaac tacgacggtc cacaggggaa aacggagcta ctcacactgc ttgccggaat
    17581 gctgcgcgag aagttgggtt gggatatcga accacagaat attgcactaa caaacggcag
    17641 ccagagcgcg tttttctact tatttaacct gtttgccgga cgccgtgccg atggtcgggt
    17701 caaaaaagtg ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga
    17761 agaagatctg tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa
    17821 ataccacgtc gattttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc
    17881 ccggccgacg aatccaacag gtaatgtgat tactgacgaa gagttgctga agcttgacgc
    17941 gctggcgaat caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc
    18001 gggtatcatc ttcagtgaag ggcgcccgct atggaatccg aatatcgtac tgtgcatgag
    18061 tctttccaag ctggggctgc ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat
    18121 catcactgcc atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc
    18181 ggcgatgatg tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat
    18241 caaaccgttt tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc
    18301 ggaaaatcgc tgcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa
    18361 ggatttgccc attacgaccg agcagctcta tcagcgcctg aaagcacgcg gcgtgctgat
    18421 ggtgccgggg cacaacttct tcccgggact ggataaaccg tggccgcata cgcatcaatg
    18481 tatgcgcatg aactacgtac cagagccgga gaaaattgag gcgggggtga agattctggc
    18541 ggaagagata gaaagagcct gggctgaaag tcactaaaaa ttgcctgatg cgctacgctt
    18601 atcaggccta catgatttct gcaatatatt gaatttggaa gaatttgtag gccggataag
    18661 gcgtttacgc cgcatctggc ataaacaaag cgcactattc cggcgttgag aaacgccgga
    18721 aaacgtttca aacaggctgc tgctgtagcc gatgccgctg cactttctcg acatccatgc
    18781 actgcaacgc ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc
    18841 acagatcgca cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa
    18901 acgggcaagc cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat
    18961 gcccatgttc gcggcttatc gcgtcaacag ggcagacatt cgcgcacggt gcatcttcac
    19021 actgatgaca ggctactggc gtggtccagc tgtggtcttt aatgacacga atacgggaaa
    19081 taaactcgtc tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag
    19141 ccacttcaca ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca
    19201 tgatcattcc tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat
    19261 ccaacgaagg aaatgttaaa gttttggcac ttgtccaaag cgttatgtga tcgcgcccag
    19321 gtcatccctg acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg
    19381 tttcaggaga tttttctctc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac
    19441 ggcgtacggc acccgcccat gaatatcaaa caccggaaca gcaatacagg agacgccgag
    19501 ttcgttttct tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa
    19561 catcgcgggc agctcggtaa tggtattgcg ggttaacggc tggatctcat gctgatggtt
    19621 ttcccagtat gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc
    19681 ggaacagtag agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt
    19741 gggttccagc ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt
    19801 ttcaccagtg gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag
    19861 cgaagacagc gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc
    19921 ggcgggcgcg gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt
    19981 actcttattt aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt
    20041 gctcaaaatc tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc
    20101 tttttcctgc gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg
    20161 tcctgatggt agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac
    20221 ttgattttta tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gtgatcgata
    20281 tatttgaaat ctagttccgc atattgaaat tttaagccaa aaaagcgatc aaaaaacaag
    20341 gaagcctagg atgaaagtga catttgagca gttaaaagca gcctttaatc gggtcttaat
    20401 ttcacgcggc gttgacagcg aaacggctga cgcctgtgca gagatgttcg cccgcaccac
    20461 cgaatccggc gtttattctc acggcgttaa tcgtttccct cgtttcattc aacaactgga
    20521 aaacggcgat atcattcctg atgcccaacc caaacgtata accagcctcg gcgcaattga
    20581 acagtgggat gcccagcgtt cgatcggcaa cctgacggcg aaaaagatga tggatcgcgc
    20641 cattgaactg gctgccgatc acggtattgg tctggtggca cttcgtaatg ccaaccactg
    20701 gatgcgcggc ggcagctacg gctggcaggc ggcggaaaaa ggctatattg gcatttgctg
    20761 gaccaactcc atcgccgtaa tgccgccgtg gggcgcaaaa gagtgtcgca tcggcactaa
    20821 cccgctgatc gtcgccattc cttccacgcc gatcaccatg gtcgatatgt cgatgttctc
    20881 ttacggcatg ttagaagtta accgcctggc aggccgtcag ctcccggtcg atggtggctt
    20941 tgatgatgag ggcaatttga ccaaagaacc tggcgttatc gagaagaatc gccgcatttt
    21001 gccgatgggc tactggaaag gttctggcat gtcgattgtg ctggatatga tcgctactct
    21061 cctttccgac ggcgcatcgg ttgccgaagt cacccaggac aacagcgacg aatacggcat
    21121 ttcacaaatt tttattgcca ttgaagtgga caagcttatc gacggtccca cccgcgatgc
    21181 caagctgcaa cgtatcatgg attacgttac taccgctgaa cgcgctgacg aaaaccaggc
    21241 catccgctta cccggccacg aatttactac cctgctggcc gaaaaccgcc gtaacggcat
    21301 cactgttgat gacagcgtgt gggccaaaat ccaggcgtta tgaggagata agtcatgatt
    21361 tttggacata tcgcgcagcc caatccgtgc cgtttgcccg ccgccattga aaaggcgctc
    21421 gattttctgc gcgctaccaa cttcaacgtc ctggagccgg gcgttgtcga aatcgacggc
    21481 aagaatattt atgcgcaaat taggtaatgc tgccaactta ctgatttagt gtatgatggt
    21541 gattttaagg tgcttgcgtg gcttccattt ccatcagatg tccttcctgc tccgctactg
    21601 aaggcgtggt gcgtaac
//
LOCUS       DAETQL010000065        21532 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.065, whole genome shotgun sequence.
ACCESSION   DAETQL010000065 DAETQL010000000
VERSION     DAETQL010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.065"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="K0W65_002730"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="K0W65_002730"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBI3095933.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="K0W65_002731"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="K0W65_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBI3095934.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="K0W65_002732"
     CDS             complement(2649..3200)
                     /locus_tag="K0W65_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBI3095935.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="K0W65_002733"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="K0W65_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBI3095936.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="K0W65_002734"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="K0W65_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="K0W65_002735"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="K0W65_002735"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBI3095937.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="K0W65_002736"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="K0W65_002736"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBI3095938.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="K0W65_002737"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="K0W65_002737"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBI3095939.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="K0W65_002738"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="K0W65_002738"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBI3095940.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="K0W65_002739"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="K0W65_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBI3095941.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="K0W65_002740"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="K0W65_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBI3095942.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="K0W65_002741"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="K0W65_002741"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBI3095943.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="K0W65_002742"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="K0W65_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBI3095944.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="K0W65_002743"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="K0W65_002743"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBI3095945.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="K0W65_002744"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="K0W65_002744"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBI3095946.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="K0W65_002745"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="K0W65_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBI3095947.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="K0W65_002746"
     CDS             complement(18170..18565)
                     /locus_tag="K0W65_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBI3095948.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="K0W65_002747"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="K0W65_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI3095949.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="K0W65_002748"
     CDS             19481..20074
                     /locus_tag="K0W65_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBI3095950.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /gene="tabA"
                     /locus_tag="K0W65_002749"
     CDS             20219..20671
                     /gene="tabA"
                     /locus_tag="K0W65_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBI3095951.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21532
                     /locus_tag="K0W65_002750"
     CDS             20794..>21532
                     /locus_tag="K0W65_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3095952.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNDSQELNNSQKLNN
                     SQELNNSQVSCKGSVDSTITDLLEKALN"
BASE COUNT         5203 a         5326 c         5673 g         5330 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt
    21241 tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag
    21301 gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat
    21361 cagaagttaa ataatataca ggagttaaat gattcgcagg agttaaatga ttcgcaggag
    21421 ttaaataatt cacagaagtt aaataattcg caggagttaa ataactcgca ggtgagttgt
    21481 aaaggttcag ttgattctac gattacggat ttattagaaa aagcattgaa ta
//
LOCUS       DAETQL010000066        21279 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.066, whole genome shotgun sequence.
ACCESSION   DAETQL010000066 DAETQL010000000
VERSION     DAETQL010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.066"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..446)
                     /locus_tag="K0W65_002751"
     CDS             complement(<1..446)
                     /locus_tag="K0W65_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HBI3095953.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(501..1352)
                     /gene="manZ"
                     /locus_tag="K0W65_002752"
     CDS             complement(501..1352)
                     /gene="manZ"
                     /locus_tag="K0W65_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBI3095954.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1365..2165)
                     /gene="manY"
                     /locus_tag="K0W65_002753"
     CDS             complement(1365..2165)
                     /gene="manY"
                     /locus_tag="K0W65_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBI3095955.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2228..3199)
                     /gene="manX"
                     /locus_tag="K0W65_002754"
     CDS             complement(2228..3199)
                     /gene="manX"
                     /locus_tag="K0W65_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBI3095956.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3662..5212
                     /gene="yoaE"
                     /locus_tag="K0W65_002755"
     CDS             3662..5212
                     /gene="yoaE"
                     /locus_tag="K0W65_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBI3095957.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5216..6814)
                     /locus_tag="K0W65_002756"
     CDS             complement(5216..6814)
                     /locus_tag="K0W65_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBI3095958.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="K0W65_002757"
     CDS             complement(6945..8309)
                     /gene="sdaA"
                     /locus_tag="K0W65_002757"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBI3095959.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8493..9071)
                     /locus_tag="K0W65_002758"
     CDS             complement(8493..9071)
                     /locus_tag="K0W65_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBI3095960.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="K0W65_002759"
     CDS             complement(9075..10436)
                     /gene="pabB"
                     /locus_tag="K0W65_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBI3095961.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10510..10689
                     /locus_tag="K0W65_002760"
     CDS             10510..10689
                     /locus_tag="K0W65_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBI3095962.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10809..11168)
                     /locus_tag="K0W65_002761"
     CDS             complement(10809..11168)
                     /locus_tag="K0W65_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBI3095963.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11530..11874)
                     /locus_tag="K0W65_002762"
     CDS             complement(11530..11874)
                     /locus_tag="K0W65_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBI3095964.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12006..13916
                     /locus_tag="K0W65_002763"
     CDS             12006..13916
                     /locus_tag="K0W65_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBI3095965.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQIVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13974..14669
                     /gene="tsaB"
                     /locus_tag="K0W65_002764"
     CDS             13974..14669
                     /gene="tsaB"
                     /locus_tag="K0W65_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBI3095966.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14709..15290
                     /gene="yeaY"
                     /locus_tag="K0W65_002765"
     CDS             14709..15290
                     /gene="yeaY"
                     /locus_tag="K0W65_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBI3095967.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15496..17181
                     /gene="fadD"
                     /locus_tag="K0W65_002766"
     CDS             15496..17181
                     /gene="fadD"
                     /locus_tag="K0W65_002766"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBI3095968.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17263..18378
                     /gene="rnd"
                     /locus_tag="K0W65_002767"
     CDS             17263..18378
                     /gene="rnd"
                     /locus_tag="K0W65_002767"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBI3095969.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18432..19397)
                     /gene="yeaX"
                     /locus_tag="K0W65_002768"
     CDS             complement(18432..19397)
                     /gene="yeaX"
                     /locus_tag="K0W65_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HBI3095970.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19453..20070)
                     /gene="yeaW"
                     /locus_tag="K0W65_002769"
                     /pseudo
     CDS             complement(19453..20070)
                     /gene="yeaW"
                     /locus_tag="K0W65_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20412..20978)
                     /locus_tag="K0W65_002770"
     CDS             complement(20412..20978)
                     /locus_tag="K0W65_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3095971.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
     gene            21190..>21279
                     /locus_tag="K0W65_002771"
     CDS             21190..>21279
                     /locus_tag="K0W65_002771"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3095972.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSRE"
BASE COUNT         5266 a         5273 c         5467 g         5273 t
ORIGIN      
        1 acgagaagct tataaatttt ttccagatcg tcgatatttc gaacgcggat taacagccga
       61 cgctgttcta attgcatcac caacacgcca tcttccgaca agttcatcgc tttgattcgg
      121 ctatattcaa tccagacatt ggcgaagaaa aaaccttttt gtttaaagat gatcttcgga
      181 atgcggatcc agaatatata aaaacccatc agagccagtg cacttaataa ccatgttgtt
      241 attagcgcgc catgattcgt gacgttgtta tagataagaa tgacaatcaa tccgacgaag
      301 ataacgctat cgatgcgacc acgccggagc aaaggaattg ccagcagggt ggggccgtta
      361 cggcggggca tgatgaactg atcgtagatc gcgaaggcca ggagtgcggc gatgaaaaga
      421 atcagtacca ggtccgtgat tgtcattaat cctccagata aaaaaacggg gccaaaaggc
      481 cccggtagtg tacgacagtc ttacagtccc agcaggccgc aagcgtaacc agcgataccg
      541 atgacgaaga agccaacgat gatccacagc gggttaactt ttttgcgcag cagccacata
      601 caagcaaatg tcagcagcag tggtacaagg cctggcatta actggtcaag aatagtttgc
      661 acggtggtga cacgggtctg tccatcctgc ccggtgatgg tcgaaaccac cagcgggatg
      721 ttgacatgtg tccacttgtt aaccaatgcc cccatgacaa acaggccgag gatagacgcc
      781 ccttccgtca gtttttgcag gaagccacca cccatatctt taacgatatc gatacctttg
      841 gagtaaccat acgctacgcc gtagtaacgg gttgccagac gcaccaggtt aaacaggatg
      901 aagaacagca gcggacctaa caggctgccg ctcatcgcga taccggcacc cagtgctgca
      961 aataccggac gtacggttcc ccagaagatc gggtcgccta caccagccag cggccccatc
     1021 aaaccgactt tgataccgtt gatagcaccg tcatcgatct ctgcgccatt agcacgctgt
     1081 tcttccagcg ccagggttac gccgagaatc ggtgcagcca cgaacggctg ggtgttaaag
     1141 aactccaggt gacggcgaat agcttgttta cgagcttcgt tgttctcagg gtagaggcga
     1201 cgaattgccg gtaccataga gaagcagaaa cccagtgcct gcatacgttc gaagttccat
     1261 gaaccctgga agaggttaga acgcaggaat acgccacgaa tatcactttg agtgagtttt
     1321 ttctcggtgg tagtttgagt tgtatcaacc atttcgctca cctgttagtc cagttcgtta
     1381 tcgagatcgt tgttaccagc tgcctgagca ggcgcaccgg ctacgcggtt gtatttcggg
     1441 ctaagttgga tgtagagcac tgccataaca gtaccaatca cacccagagc aaccaggtta
     1501 aagttggtga atgctgcggt tacgaagccg aggtagaaga acggcatcag gtagccagca
     1561 cgcatcatgt tgataaccat cgcataacca accaccacga tcatgccccc ggcgatattc
     1621 agaccattgg tcaccacttc cggaatcgca ttcagcatgt tctgtacttc gctggtacca
     1681 acagacagcg caacgatgac ggccggaata gccacacgca ttgcttgcag gaacagagaa
     1741 gaaacgtgga tccaggaaat cgctgtcagg ttgccgttat cagcagcctt atccgcagcg
     1801 tgctggaaag caacggtaat agtacgaacg atgatggtca gtacctggcc cgcagcggcc
     1861 agagggattg ccagtgcgat acctgcacca atgctctgat gacctgcgat aaccagaatg
     1921 gtagaaatga tagaagccag agcggcgtca ggcgcaactg cagcaccgat gttcatccag
     1981 cccagcgcga tcatttccag cgtaccaccg ataataatac cggttttcat atccccaaga
     2041 acgataccca ccagggtaca cgcgattagc ggacggtgaa actgaaattc atcgaggatt
     2101 gatcccatac ctgcgataca ggctacgata aataccagca caatttgaag agtggtaatc
     2161 tccattgtac ttctcctgtt tacgtaagac ttaagtgtga aaactgaatg ataatcaaca
     2221 caatacgtta cttatcgatt ttgctgatca gatccatcat tttcagtttc ggatcggtgg
     2281 aaaccttacg gacttccagc tcaataccgc gtgcattcag ttttttgaac gcctcgatat
     2341 ctttttcatc aaccgaaacc gcgttattca cctgggtttt accctgacgg aatgccatac
     2401 caccgacgtt aacagaggtg attttcacgc cgccttcaac gagacgctct acatctgttg
     2461 ggttggtaaa taacagcatt acgcgttcgc cagcatattt cgggttgttg tagacgcgaa
     2521 tcattttggc aacatcaact acgtgtgctg ttacgcccgg aggtgcaacc tgggtgagca
     2581 gtgtcttacg aacggtatcc gcagccactt catcactaac aacaataata cgggagacat
     2641 tggtttcttt ggtccagcgg gtggcgacct gaccgtgaat cagacggtcg tcgatacgcg
     2701 caaggccaat aaccatgtag tcgtttggcc ccattggttt tgccggagtt ggagccgctt
     2761 ttggtgctgc ggcagcgggt gctggcgcgg ctttttcaac cggtttggct ttcagtgctt
     2821 tcacgccttc acggcctgtt tctactgcca gcgcaaccag ttcatcaaag ctcgggtcat
     2881 catcacgggc cattaacgtt tccacgagca ttggaatgtt aacgcctgca atgacttcat
     2941 aatgctcttt gtcgacgaca atgcggctgg cagcattgaa cgggctgcct ccccatgtat
     3001 caacgagaaa cagcacgcct ttagtggtgt cgagttttgc caactgagcg ttgtattttt
     3061 caatcagcgt ttcggcattt tcacctggaa cgaaatcgat ccagccgacg ttttcctgct
     3121 cgcctaacag catttctgcc gttttaagca actgctctgc agcccaacca tgtgtgccta
     3181 taacaatagc aatggtcact tgctacctcc tttattatcg ttaacacctc aacgtgccag
     3241 atgtattttt gaatcgtcgt ccacaatcga atcgattcag ataagggcta caaccaaaaa
     3301 cccctgcgtt tcgcgattta ttttagatat cgaaaaaatt attttatgtg atgaagatcc
     3361 gtaatttaac tttcgattat cagaaatttc gaaaggtaaa atatccttgt cacattcgtt
     3421 tgcaaaggaa ggtaaatctt tgccaaatca gaggcgtctc tgatatgttt aactcccgtt
     3481 taacaaccat ggagtatagg gcagtagccc gcagtatgga tcgtcaccga cgtcatttca
     3541 gcatcaggcc ttttaacgcc tgcctttctg gcactctatg ccgcaccttt cgtttgcatt
     3601 ttgtcgttac gcctgcatta tttctggcgt cgaatagcta ttccttaagc aggagcttgt
     3661 catggaattc ttaatggacc cctcaatttg ggcggggcta ctcacgcttg ttgttctcga
     3721 aattgtgctg ggtatcgata acctggtctt catcgccatt cttgctgaca aactgccgcc
     3781 aaaacaacgc gataaagcgc gtttgctggg gttatcactg gcgctgatta tgcgtctggg
     3841 gctgctgtcg ctgatttcat ggatggtcac gctgaccaaa ccgctattta ccgtcatgga
     3901 tttctccttc tccggacgcg atctgattat gttgttcggg gggatattct tgctgttcaa
     3961 agcaacaacc gaactgcatg aacggctgga aaaccgcgat catgattccg gccacggtaa
     4021 aggctacgcc agtttctggg tggtcgtcac acagattgtc atccttgacg ccgtcttctc
     4081 gttggatgcg gtaattactg cagtagggat ggttaaccat ctgccggtga tgatggcggc
     4141 ggtagtgatt gcgatggcgg ttatgttgct ggcatcgaaa ccgctgacgc gattcgttaa
     4201 ccagcacccc acggtggtgg tgctctgtct gagcttcctg ttaatgattg gtctgagtct
     4261 ggtggcagaa ggtttcggtt tccacattcc gaaaggttac ctgtatgccg cgattggctt
     4321 ctcgatcatt atcgaagtgt ttaaccagat tgcgcgtcgc aactttattc gtcaccagtc
     4381 gactttgccg ctgcgagcgc gtactgccga tgccatcctg cgtttgatgg gcgggaaacg
     4441 tcaggccaat gttcagcacg atgccgataa cccgatgccg atcccggaag gtgcatttgc
     4501 cgaagaagaa cggtacatga ttaacggcgt actgacgctg gcgtcgcgtt ctctgcgcgg
     4561 gatcatgacg ccgcgcggtg aaataagctg ggttgacgct aatctcgggg tcgatgaaat
     4621 ccgcgagcaa ctgctctctt caccgcacag tctgttcccg gtatgtcgcg gtgaactgga
     4681 tgaaatcatc ggtatcgtac gtgctaaaga actgctggtg gcgctggaag agggcgttga
     4741 tgtggcggcg attgcttcgg cgtctccggc gattatcgtc ccggaaaccc tcgatccgat
     4801 caacctgctg ggcgtgctgc gtcgcgctcg cgggagcttt gttatcgtga ccaacgagtt
     4861 tggtgtggta caaggcttgg tcacgccgct ggatgtgctg gaagccattg cgggtgaatt
     4921 cccggacgct gacgaaacgc cggaaatcat taccgatggt aacggctggc tggtaaaagg
     4981 cggtacagat ttgcatgcct tgcagcaggc gcttgatgtt gagcaccttg ccgatgacga
     5041 tgatatcgcg acggtcgcgg gcctcgtgat ctcggcaaat ggtcacattc cccgtgtggg
     5101 cgatgtgatt gatgtagggc cactgcatat caccatcttt gaagccaatg attatcgtgt
     5161 tgatctggtt cgcattgtta aagagcaacc ggcgcacgat gaagatgagt aagcgttaac
     5221 gtaacgacat aatgggcgtg atatgtccat tatgccgggc gggcggcggt tggctgcccg
     5281 ccagccattt gggaaaatca cgtagcggca tcggctgtgc gtacaaatac ccttgcaaaa
     5341 tatgcacgcc atgacggcgc aaatacttcg cctgttcttg cgtttccacg ccctccgcca
     5401 ccagttcaat attcagtctt tgccccagcg cgatgatgat atcggtcacc gtcgagttaa
     5461 ccgcgtcaga acctatagct gcggtaaatg acttatcaat tttcagcacg tcaggacgta
     5521 atgtttcaag ccaggaaaac gagctgttgc cggtgccgaa gtcatcaatc gccagtttga
     5581 cgtttttacg atgcagctcg cgggcaatcc gataatcaac atccagtaag gcatcgcgtt
     5641 cggtgatttc gaggatcagt tgctgaatcg ggtgagcgct aaaccagtac tgattgagat
     5701 cttttatcag cacaccacgg cgaaaatggc tgggtgcgac gttaatgcca acatgaaact
     5761 gactactcat cgggaaaaca tggtgctgac gaatggtttc tgccatcaca tagtgggtca
     5821 gtggcacaat taaatgatgt tcttccgcga taggaataaa cacatccggt gaaatccagc
     5881 cctgacgcgg attattccag cgcagcaaaa tctctacacc aatacattgc tggctgcgcg
     5941 cattaagcaa aggctgacag aacaattcga actcatgttg cgccaggccc agattaattt
     6001 cgcgggaaaa gctcatccgg taagcggtcg ccagccaggc gatatagccc accagtaaac
     6061 ttagcagcac cgccagcggt aattgtgttg gaagatagtg ccatgccagc gccgtagccc
     6121 ccggaccatt tacgttaatg gtaaaaggaa agctttgcga agaaacctga tagttttcat
     6181 tgtcttcagg ttgcggaagg gaatctacca gcccattacc atagagcaaa tgccgtttgt
     6241 ccaccgtcag gctggcgcta ctgatttgcg gcagttgtgg ctcaaggagc attgccgtca
     6301 gtaagtcgat gttaatcatc tctatgaccc cagcattgct actgcccgct gcaggcgtcc
     6361 attgaatcaa aaccggactg cctttaatca gggcacggtc gatcgtcagg cgtaaaagtg
     6421 gttgcggtgc aggaagttca gccagaatgt ccacgacggg cacattgcga taaccaaaaa
     6481 tgctggagca ataaagtgtg ccgtcttgca ccaggccaat ggatcgcaca gtttggagtt
     6541 ttgccgcctg tttacgtaat ggcaaatggg cgacagagca gggcagacca atcagcggaa
     6601 gcaagacttt gctacctgcc tgtaggggaa gcagtacttt atctaattcc tctacagcgt
     6661 gattggcgaa ttgtactacc cgttgttgat ttaagttacg ctgtgaaata aatcgcacgc
     6721 tcagggtcga agcgagcgta acgagtgcgc aaatcgtaca aacaatcatt cgattacggc
     6781 gataggtttt aatgatccgt tgtgcttttt gcatgcgggc caccctgtaa gcgtatggcg
     6841 acaaacgcct gacgcaacag tggaagtgta gtggggaata ttacagcaga cgagaaagcg
     6901 ggtataaatt cgcccatccg ttgcagatgg gcgagtaaga agtattagtc acactggact
     6961 ttgattgcca gaccaccgcg tgaggtttcg cggtatttgg cgttcatgtc cttaccggtt
     7021 tcgtacatcg tttcgatgac cttatccagc gagacgcgcg gtgcactggt gcggcgcaga
     7081 gccatccgtg cggcgttaat cgccttcaca gaggcaatgg cattacgctc aatgcacggc
     7141 acctgaacct gccctgcaac cgggtcgcag gttaaaccaa ggttgtgttc catgccaatt
     7201 tccgccgcca cgcaaacctg ttccgggcta ccgcccagca gttctgcaag acccgcagca
     7261 gccattgaac aggcaacacc cacttcgccc tggcaaccaa cttccgcacc ggaaatagag
     7321 gcgttcattt tatacaatgc accaatcgcg cccgctgcca taaagtaacg ggtatagatg
     7381 tctgggctga ccgattcaat aaagtggtca tagtaagcca gcactgccgg aacgataccg
     7441 caggcaccgt tggttggcgc agttaccaca cgaccaccgg cggcgttttc ttcgttaact
     7501 gccagcgcaa acatgtttac ccagtcaatg acattcatcg gatcgttaga cagtttatcg
     7561 ctggaaacta acatccggcg cagggcagac gcacgacgtg gcacgcgcag cgggcctggc
     7621 agtacacctt cggtgttcat cccgcgatcg atacatgcct gcatggtttg ccagacatgc
     7681 gcgaaatact cgtcgatctc tttcttgctg tgcagcgcca gttcgttctg catagcgaga
     7741 ccagacagcg aatagccggt ttcattacag tacgcgagca gttcggtggc agatttgaac
     7801 ggatacggca cgctcacttc gttggcagca tcctgaccaa agtgttcttc atcgacgata
     7861 aaaccgccgc cgatggaata ataagttttg ctgtagacga cttcatcgcc gttataggcg
     7921 tggatttgca taccgttttc atgcagcggc aggttgccgt tatgaaaacg catcccgttg
     7981 tcgcgcggga aatccacttc atgccgtccc tgtgccagca gcagacgttc gcgctctttt
     8041 acgtcgcgaa taaaaccggg aatactgtcg atatccacgg tggcaggttc gttacctgca
     8101 agacccataa taatggcgat atcggtgtgg tggcctttac ccgtcagcga cagtgaacca
     8161 taaacgtcca cggcaacgcg agtaacgcta tccagtaagc ctttttcgac cagatcatcg
     8221 acgaactgtt tacccgcctt cataggccct acggtatggg aagatgaggg accaatcccc
     8281 accttaaaca tgtcgaatag actaatcacg ataatactcc tgacaaggtg actggacttc
     8341 cagtaacgaa ctaataacag cgcatagtgt aagagggaac gcggcgactg gcttaactat
     8401 tcacatgaat taaactaatc aaaaaccgcg atttaactaa tattgcgatg attgtctcaa
     8461 gtagtgtttt gtagatgtaa agataagtat agtcaggggg tcacaccaat ttgcagcgcc
     8521 agctcacgaa ttatgcctgc ggtcattccc catacaaaat actgttcgta ccaggatagc
     8581 catacccgat gtgaatcgcc acggcggtag atatctaaag ggtgataacg acccagatgt
     8641 aatgcctggg cgagcggcat ttcaaacacc gccgagactt catcttcact ggcgcgatac
     8701 ggcagatcgg gcgggataat gccgaccact ggggttactt gatagccagt tacgctatcg
     8761 acgggcggca gcacgccgat aacttcaacg gcggaaggcg gtatagcgac ctcttcttca
     8821 gcttcgcgca gcgcggcggc gataactgat gcgtccgtgt catcgactgc acctccaggg
     8881 aatgccactt gtccagcgtg tttacgcaga tgaattgaac gctgagtcag caacaacccc
     8941 ggttgcggtc gacggacgat ggggattaac acggcagcct gacgatgatt taggggttcc
     9001 cggttaattt gcgggcgcaa aagttgaaag cgagataaaa aatcatcaag cgtcaggcta
     9061 cggtattcca cgtcttactt ctccagttgg cgtaatatct tattaacttt atcaaaagtt
     9121 tcctgatatt ccgcttcttc ctggctatcg gcgacaattc caccgcccgc agagcagtat
     9181 atttgtccgt taatggcagt cagcgtgcgg atagtaatgc tggtatccat gttgccgcaa
     9241 aagctcaaat agccaatgct gccgcaccag gcattacgtc gctgcggttc cagttcgtcg
     9301 ataatttcca tagcccgtac tttcggagcc ccggttattg agccaccagg aaaagctgcg
     9361 cgcagcagat cgctggcgtg taactgttct ggtagtcgcg ccgttatagt gctgaccaga
     9421 tgatgcacgg cagggaaggg ttccaccacg aagagctctg gtacttttac cgaaccggct
     9481 acggcaacac gaccgatatc attacgcatt aaatcgacaa tcatcagatt ttcggcacga
     9541 tctttcgctg agttcgccag tttttctgct tgtttgctat cttcctgagg atcgggcagg
     9601 cgtggtagcg tgcctttaat cgggcgagtc tggatttcac tattatcaca aagaataaac
     9661 cgctctggcg aaaggcttaa aattgcaccc tgttcaagac gtaaaaaagc gctaaatggc
     9721 gcgcggttgg cctgattaag ctgaaggaat gcctgccatt catcgccaga ataggtcgca
     9781 tgaaaacgct gggcgagatt cacctgatag caatcaccgc tgtgcagata ttcctgtacc
     9841 tggcgaaatt tttcgccgta ctgctcgtgg gtcatattgg attgccagtc ggaagtgagc
     9901 gtgaaatctt cctgcggcga gaattgctgg ctttccagcc aggcccgacg ggcattgaca
     9961 tcattatgac tcagcaaaga aactgtatga cgctggtggt cgacaatgag cgcccaatcg
    10021 tagataccca ctgccatatc cggcagaacg atatcttgtt ccgcaatttc tggcagtgac
    10081 tcaaaacggc ggcccagatc gtagccaaac aaccccagtg cgccgccctg aaatggcaaa
    10141 tcttcgttat gcgttgggcg aatgtctgcg cgatccagca cctgctggag cacctgtagc
    10201 gggtcatcag tggtcgttgt gcgtttttcg ctttcactaa caacggtttc tttacctaaa
    10261 gtggttaaag tgcaaatcgg atcggcgacc acaatatcaa agcggctata cggatgatcg
    10321 gcatagccgg agtgtaaaag catcgcccac ggcaggtggc ttaagcggga gaaataaaat
    10381 tcagcggcgt cctgacgcca gggtaaagta atcacagtgg gagataacgt cttcatcagt
    10441 cctgactcta ctggctatgt gccgggtagc atgaaataat tagcgccaac aatttagcag
    10501 gagttaacaa tgtttgcagg tttaccttca ctcatccatg aacagcagca aaaagctgtc
    10561 gagcggatcc aggaactgat ggcacaaggg atgagtagcg gccaggcaat cgcgctggtg
    10621 gcagaagagc tgcgtgccaa ccactccggc gagctaatcg tggcgcgttt tgaggatgaa
    10681 gacgagtaac acactaatcc gtatcgattt cgttatagaa gccgatgatc atttgattgt
    10741 gagaaaaagg acgcttttta tatgtgatta aaagcgtcct gacccattta tgctgatata
    10801 aatttaactc agaccagtgc ttttagttct tcctgcatat atgttgaaaa gtattcagga
    10861 tttttaatca cagagcgttc acctgtctcc attttttttg cccatccaac cattttttcc
    10921 gtgaacccag ggttccagcc ttcctggtca gctcgcgccg taatatcagc ggcactcgcg
    10981 ggtactccca gttcttttaa atatttaaag attcccttcg ccgtgctttc attcatcgaa
    11041 cttggtgttg gcgctgatac atccatggca ccaatgaaat ccagggcttt atctattaca
    11101 gcaggcatat tcttatcctt aaaattaacc atgttatcga taaggctata tatgacctta
    11161 atttgcatct aatatcaaag ggattataac gatatggcac gagttaacct gttgacattc
    11221 aggtgtttta aaggtagcag ctcatggtgt gttatccagt aagatggaga aaattaacgg
    11281 tcagcccgca cgctataacc agattgttca gctaagaaag aaaaccagag ttgctaatca
    11341 aagggcgaac acaacggcat ggttatgttt atttatatag ttagttttat agataaaatt
    11401 gaaattataa atataaataa tgaattagca gaagcatcaa cagtaatatc ttattctctg
    11461 gtcgatttat gtaaaaccga ccagtattat cgataaatta tcgtctgact tattttgctt
    11521 cgataaagct tacaccgcag ccacaatctt aatttcaact ttatacttcg ggttcatcaa
    11581 acccgcttgt accgtgcagc gcaccggcgc atgacccgcg acaacccagg catcccacgc
    11641 tttattcatc gccgcgaagt cgtttttatc ggccaggaaa atggtggcat ccagaatgct
    11701 cgatttattg ctgccctgtt tttccagcac ggcgtcaatc tgtgccagcg tgttggcggt
    11761 ttgctcaaag gcatcggcgt cgaggttttc cggtacacca gtgtagtaga gcgtgttgtt
    11821 gtggattact acatcagacc agcgggcttc agcatcgata cgaacgatag tcatcatttt
    11881 gtcctcatta tacttccatt ttcgtgctgc aaagactgcc acaatgtgag gttgccgtca
    11941 ccacttcaac tggcgaaagc gccctcatcc tgacataatc cctgttcaaa tcaacagggg
    12001 gtagtgtgac ggacgatttt gcaccagacg gtcagctggc gaaagcgata ccaggcttta
    12061 agccgcgaga accacagcga cagatggcgg tagccgtcac ccaggcgata gaaaaaggcc
    12121 agccgctggt ggtggaagca ggaaccggta cgggcaaaac ctacgcttac ctggctcctg
    12181 cgctgcgggc gaaaaagaaa gtcattatct cgaccggctc aaaagcgttg caggatcagc
    12241 tctacagccg cgatttgcca acagtctcaa aggcattgaa atatacgggc aacgtggcgc
    12301 tgctgaaagg gcgctcaaac tacctctgcc tcgaacgtct cgaacagcag gcgctggcgg
    12361 ggggcgatct gccggtacaa atcttaagcg atgtgatcct gctgcgctcc tggtctaatc
    12421 aaatagtcga tggtgatatc agcacctgcg tcagcgtggc ggaagattca caggcgtggc
    12481 cgctggtcac cagcaccaac gacaactgtc ttggcagcga ctgcccgatg tataaagatt
    12541 gctttgtggt caaagcacgt aaaaaagcga tggacgccga tgtggtggtg gtaaaccatc
    12601 atctctttct ggcggatatg gtggttaaag agagtggatt tggcgagttg atccctgaag
    12661 cggacgtcat gatcttcgac gaagcccacc agctaccgga cattgccagc cagtattttg
    12721 gtcagtcact ctccagtcga caactgctcg acctggcaaa agacatcacc atcgcctacc
    12781 gcaccgaatt aaaagacacc cagcagttac aaaagtgcgc tgatcgtctt gcccagagtg
    12841 cgcaggattt tcgtctgcaa ctcggtgagc caggttatcg cggtaacctg cgtgagctgt
    12901 tagctaatcc gcaaattcag cgggcatttt tactgctcga tgacaccttg gaactttgtt
    12961 atgatgtggc gaaactgtca ctggggcgtt ccgccttgct ggatgcggca tttgagcgcg
    13021 ccacgttgta tcgcacacgg ctgaagcggc taaaagagat caatcagccg ggctacagct
    13081 actggtacga atgcacttcg cgccatttta ctctggctct cacgccgctc agcgtggcgg
    13141 ataaattcaa agagttaatg gcgcaaaagc ccggtagctg gatcttcacc tcagcaacgc
    13201 tgtcggtgaa cgacgatctg catcatttca cctcgcggct tgggatagaa caggcggagt
    13261 cgttgctatt gccaagcccg tttgattaca gccgccaggc gttactctgt gtgccgcgca
    13321 atctgccgca aaccaaccag ccaggttctg ctcgccagtt agcggcaatg ctgcgaccga
    13381 tcatcgaagc taacaacggt cgttgtttta tgctttgtac ctcgcacgcc atgatgcgcg
    13441 atctggccga gcagttccgc gctaccatga cgcttcccgt tttgttgcag ggggaaacca
    13501 gtaaaggcca actgttgcag cagtttgtca gtgccggtaa tgcgcttctt gtggcaacca
    13561 gtagtttctg ggaaggggtg gacgtgcgtg gcgatacatt gtcattggta attatcgaca
    13621 aattgccgtt tacctcgccg gatgatccac tgttaaaagc gcgcatggaa gattgtcgtt
    13681 tgcgcggtgg cgacccgttc gatgaagtgc aactaccaga tgccgtcatt actctcaaac
    13741 agggggtagg tcgactgatt cgcgacgccg acgatcgtgg cgtgctggtg atttgtgact
    13801 atcggctggt gatgcgtcct tacggcgcga cgtttctcgc cagtctgccg cccgcgccac
    13861 gcacccgtga cattgcccgt gcggttcgtt tccttgcgat accatcctcc aggtaatttg
    13921 ttacggagta tggtatgatg cgcgcctttt tattgatcta agcacgaaga cccatgcgaa
    13981 ttctggctat cgataccgcg acagaggcct gctctgtcgc cctgtggaac gacggtactg
    14041 tcaacgctca ttttgagctt tgccctcgtg aacatactca acgaatctta ccgatggtgc
    14101 aggatatcct gaccaccagc ggaacttccc tgactgatat taacgctctg gcttacgggc
    14161 gcggccccgg tagctttacc ggcgtgcgca ttggtattgg catcgcgcaa gggctggcgc
    14221 ttggcgcgga attaccgatg attggcgtct ccacgctaat gacgatggcg caaggggcgt
    14281 ggcgcaaaaa cggcgcaaca cgcgtgctgt cagccattga tgcgcgaatg ggcgaagttt
    14341 actgggccga atatcagcgt gatgaaaacg gtatctggca cggtgaagaa accgaagccg
    14401 tactcaaacc cgaactcgtt catgaacgaa tgcaacagct ttccggcgaa tgggtgacgg
    14461 tagggacggg ctggcaagcc tggccggatc tcggtaaaga gagcgggctg gttttgcgcg
    14521 atggcgaagt gttactcccc gctgcagaag atatgctgcc gattgcttgt cagatgtttg
    14581 ccgagggtaa aacggtggcg gtggaacatg ccgagccggt ttatttgcgt aacaacgtcg
    14641 catggaagaa acttccgggc aaagaatgaa tcttaagagt atgccctgag aaaaaaagga
    14701 gtcgcatcat ggcggttcaa aagaatgtta tcaaaggcat actggcaggt acgtttgcgc
    14761 taatgctgag cggttgtgtc actgtgccgg acgccattaa aggcagcagt accacgccgc
    14821 aacaagattt agttcgggtg atgagtgcgc cgcagctgta cgttggtcag gaggcacgct
    14881 ttggtggcaa agtggttgcg gtacaaaacc agcaagggaa aacccgcctg gaaattgcta
    14941 ccgtaccgct ggacagcgga gctaggccga cgctgggaga accttctcgc ggtcgcattt
    15001 atgccgatgt gaacggtttt ctggacccgg tggatttccg tggacaactg gttacggtag
    15061 tcgggccaat caccggtgcg gttgacggca aaatcggcaa tacgccctat aaatttatgg
    15121 tgatgcaagt aacgggttac aaacgttggc atttaaccca gcaggtgatt atgccgcctc
    15181 agccgattga tccatggttt tatggcggtc gtggctggcc ctatggctac ggcggatggg
    15241 gctggtataa tcccggcccc gcgagagtac aaacagttgt aaccgaataa ttgcttgttt
    15301 ttaaagaatg cagaaacagc ggctggtccg ctgtttctgc attcttacgg taaagataaa
    15361 aataaatagt gacgcgcttc gcaacctttt cgttgggtaa ttatcaagct ggtatgatga
    15421 gttaatatta tgttaacggc gtgtatatca tttggggttg cgatgacgac gaacacgcat
    15481 tttagaggtg aagaattgaa gaaggtttgg cttaaccgtt atcccgcgga cgttccgacg
    15541 gagatcaacc ctgaccgtta tcaatctctg gtagatatgt ttgagcagtc ggtcgcgcgc
    15601 tacgccgatc agcctgcgtt tgtgaatatg ggggaggtga tgaccttccg caagctggaa
    15661 gaacgcagtc gcgcgtttgc cgcttatttg caacaagggt tggggctgaa gaaaggcgat
    15721 cgcgttgcgt tgatgatgcc taacttattg caatatccgg tggcgctatt tggcattttg
    15781 cgtgccggga taatcgtcgt aaacgttaac ccgttgtata ccccgcgtga gcttgagcat
    15841 cagcttaacg atagcggcgc atcggcgatt gttatcgtgt ctaactttgc ccacactctg
    15901 gaaaaagtgg ttgataaaac cgccgttcag cacgtaattc tgacccgtat gggcgatcag
    15961 ctatctacgg caaaaggcac ggtagtcaat ttcgttgtta aatacatcaa gcgcttggtg
    16021 ccgaaatacc atctgccaga tgccatttca tttcgtagcg cactgcataa cggctaccgg
    16081 atgcagtacg tcaaacccga actggtgccg gaagatttag ccttcctgca atacaccggc
    16141 ggcaccacag gtgtggcgaa aggcgcgatg ctgactcacc gcaatatgct ggcgaacctg
    16201 gaacaggtca acgccaccta tgggccgttg ctgcatccag gtaaagaact ggtagtaacg
    16261 gcactgccgc tgtatcacat ttttgccctg accattaact gcctgctgtt tatcgaactg
    16321 ggtgggcaga acctgcttat cactaacccg cgtgatattc cagggttggt aaaagagtta
    16381 gcgaaatatc cgtttaccgc tatcactggc gttaacacct tgttcaatgc gttgctgaac
    16441 aataaagagt tccagcagct ggatttctcc agtctgcatc tttccgcagg cggtgggatg
    16501 cctgttcagc aagtggtggc agaacgctgg gtgaaactga ccggacagta tctgctggaa
    16561 ggttatggcc tgaccgagtg cgcgccgctg gtgagcgtta acccgtatga tattgattat
    16621 catagcggta gcatcggttt gccggtgcca tcgacggaag ccaaactggt ggatgacgac
    16681 gacaacgaag taccaccggg tcaaccgggt gagctttgtg tcaaaggacc gcaggtgatg
    16741 ctgggttact ggcagcgtcc ggatgctaca gatgagatca tcaaaaatgg ctggttacac
    16801 accggcgaca tcgcggtgat ggatgaagaa ggattcctgc gcattgtcga tcgtaaaaaa
    16861 gacatgattt tggtttccgg ttttaacgtc tatcccaacg agattgaaga tgtcgtcatg
    16921 cagcatcctg gcgtacagga agtcgcggct gttggcgtac cttccggctc cagtggtgaa
    16981 gcggtgaaaa tcttcgtagt gaaaaaagat ccatcgctta ccgaagagtc actggtgact
    17041 ttttgccgcc gtcagctcac gggatacaaa gtaccgaagc tggtggagtt tcgtgatgag
    17101 ttaccgaaat ctaacgtcgg aaaaattttg cgacgagaat tacgtgacga agcgcgcggc
    17161 aaagtggaca ataaagcctg agcgttaagt cagtcgtcag acgccggtta atccggcgtt
    17221 ttttttgacg cccactaaag agaaaacaat ttgaattacc aaatgattac cacggacgat
    17281 gcgctggctt ctttgtgtga agccgtccgt gtctttccgg cgatagccct ggatactgaa
    17341 tttgttcgta cgcgcactta ttacccgcag ctggggttga ttcaactttt cgatggcgag
    17401 catctggcgc taattgatcc tctcgggatc accgactggt caccgctgaa agcgatcctg
    17461 cgcgatccgt ccatcacaaa atttctccat gcaggcagtg aagatctgga agtgttcctc
    17521 aatgtctttg gcgaattacc acaacccttg attgacacgc aaatccttgc tgccttctgc
    17581 ggacgcccga tgtcatgggg tttcgcttcc atggtggaag agtattccgg cgttacgctg
    17641 gacaagagtg aatcgcgcac cgactggctg gccagaccgc tgaccgaacg tcagtgtgaa
    17701 tacgcagcgg cggatgtctg gtatctgtta ccgatcaccg ccaagcttat ggtagaaacg
    17761 gaggcctccg gctggctacc tgcggcgctg gatgaatgcc gcctgatgca aatgcgtcgt
    17821 caggaagtcg ttgcgccgga agatgcctgg cgtgatatca ccaatgcctg gcagttacgc
    17881 acacgccaac tggcctgtct gcaactgtta gccgactggc gactgcgcaa ggcgcgagag
    17941 cgtgatctgg cggtgaactt tgtcgtgcgt gaagagcatt tgtggtcggt cgcgcgttat
    18001 atgccgggaa gtttaggcga actggacagc ctgggtttat ccggtagcga aatccgcttt
    18061 cacggtaaaa cgctgctggc gctggtggaa aaagcgcaga cattgccgga agatgcctta
    18121 ccgcagccga tgcttaacct gatggacatg cctggttatc gtaaagcgtt taaagcgatt
    18181 aagtcgctga ttactgacgt gagcgaaacg cataagatca gcgccgaatt gctggcatcg
    18241 cgtcggcaaa tcaaccaact gctgaactgg cactggaaac tgaaaccgca gaacaatttg
    18301 ccggagctga tttccggctg gcgtggtgag ctgatggcgg aagcattaca caatttattg
    18361 caggaatatc cgcagtaaaa tcttccgaag ccggactggg cgcgctcagc gccacatccg
    18421 gctttggcaa actacaaatc caacaccagg cgtttaccct tcgcacgcga acaacagatc
    18481 aacatacttt gctggctggc acgctcttca tcgctaaaat actgatcccg atggtcagct
    18541 tcaccttcca gtattgccgt ttcgcaggtt ccgcataccc cttcacgaca taaacattcc
    18601 actttcgcgg ctttattatt ttcaataacc tgcaaaatag tcatctcttc cggcaccaca
    18661 aactcttttc cggaacgggc aagcaccagg gtaaatgcat cgccggtttt gtcttcgata
    18721 gcaaattgct caaagtgcag tgtatcggcg gcgatgtcca gacgcgcagc ttcacttctt
    18781 accgcttcaa ttagcgcctc ggggccacag gtataaacgt gtgtgccagg ttcgatatcc
    18841 gccaataatc gcgctaattc cagtcgtgtt ccggttgatg aatgatgcaa atggactttc
    18901 tcagcctgcg gatgctggac tagctcagca cgaaatgcgc aactttctgg atttcgcgag
    18961 cagtaatgta gctgccagtc gatgtcgctg tgttgcaact ctgccatgtg cgacaggaaa
    19021 ggggtgatac caataccgcc cgcgataaac agatgctttc tggctgaggg aattagcgca
    19081 aacaggttat taggcgttga gatcgttaac cgatcgccca cttttacctg ctgatgcaaa
    19141 aagcgggaac cgccgcgcga gttttcttcc agtcgaacgg caatctgata actagaggtg
    19201 ttatgcggtg aactcagtag tgaatacgca ttgctgtact ggttatcacc atcgctcatc
    19261 tgcactagga tgtgacttcc tccggtgaac gcaggcagcg gtttgccatc ggttgccacc
    19321 agcgtgaagc gtttcacctg ttcggtaagg ggttcaacct ggctcacctg tacttcaaac
    19381 atttgatagt ctgacattgc tgtactccat caaaaatcag cccagcggcg gaaaataccg
    19441 ccgccgatgt cattagtcct taaacacctg cgccagcaga ttatggaaat gggcgatacc
    19501 atgttcggaa atgccactac cgctactgtc ggccatgatg cgcccctgac cacgatagcc
    19561 acgcgatttc agccctttct gtacgctttc aaccagacgt aaatcttccg gatggaacac
    19621 atcgcgatac cactcaatca gcgatttttg ctcgtcggtt aactcttcat tggtgaagta
    19681 aatatcgtag ttttgcaggg tagtttcaga atccaccggg aattcataaa tgaccgtcat
    19741 catccctttg atcggggtga cgttcagcat cgtgcacggc cacagccaga aaccgtggaa
    19801 tgccgcatcc gtaccctctt caaatttaaa cgactgttcg gacggtttgg cgaaaccgta
    19861 ttgcagcgtc cagttaccgt gcatggtgtg ccagtaacga tcaacctgta cggagtcgga
    19921 gaaacctaga tgcgccggac cacagtgata gcactcgaga tagttatcga caatgttctt
    19981 ccagttggca ggcgtgcggg tggtaaagcg ggccgctagt ttcagattgt ggacttccgg
    20041 gcaggcttcc agcactttcg cgcccagacc ggtgtattga ttcacttgaa gtacgaaaaa
    20101 aaaccgggag gacattggat tattcgggtt ctgatgggat tagatttggt ggggcttgca
    20161 agcctgtagc gcaaatttta gtcgttaatc aatgaaacgc gaaagatagt aaaaaattgc
    20221 ttttgtttca ttgaaaatac gaaaaacaaa aacactgcaa atcatttcaa taacagcttc
    20281 aaaaaacgtt caaaaccgat aacaaccagg ctgtaaccaa atgactcata tcacaaatca
    20341 gctcatgccg tttaggtatg ttacatgtgt gattatgtga ggtgaagtat gttttagctg
    20401 gttcatggtt gttatacggc tttttttaca tcttgtggtt cttgtgaagg agctacaaga
    20461 ccttcctgtt cataaatacc atactttgac aaaatctttt tacgagtatc tgttggtaat
    20521 gattcaagca ttttggctgt ttgtaaggtt tgctgcgaaa taccttgcaa atcagcctgg
    20581 atggaatcat cggaagaaaa aatctcaggc ataagagctt caaatccttt ctttctgaac
    20641 aactcaatcg cagaatgcag ttccttgtcc tctaacaatt cgacaattgc gatcagttta
    20701 gttttcaatg tgctgcggtc aggttgagaa tttttattct cttgattatt tttatgctca
    20761 accattggca tgctctgact ttggataaac cactctattg gacgccctgt tgcgtaggct
    20821 atttgtgcta atcgatctaa agacgggtat gtcttgccag acaggtagtt tctgataacc
    20881 ccatcagaaa gtccgcactt agctgcaaac gcccggcttg attctccatt cattgcctct
    20941 tcaaggcgtt ccttgaaacg cattatttcc gggggcataa aaatgcgctc tgcatttttg
    21001 ctttcttttt cacctgtcat gcccaatctc tctgttatat aaagaatttt acagaattct
    21061 ttgatttgca ctggttaaaa taaaaatgcg catagctttt gaattgagca tttttctgcg
    21121 ctatcgtttt tcccgatagc ggtagccggt atcagttacc gaaaacgtta agagagtaag
    21181 gatcgcagaa tgtcagcaaa aaaccaagac tggcacgcag agcagataaa ggctgctgtt
    21241 cgtatgaagg ggaagacact atctcaatta tccagagag
//
LOCUS       DAETQL010000067        21256 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.067, whole genome shotgun sequence.
ACCESSION   DAETQL010000067 DAETQL010000000
VERSION     DAETQL010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.067"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            21..794
                     /locus_tag="K0W65_002772"
                     /pseudo
     CDS             21..794
                     /locus_tag="K0W65_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(854..2152)
                     /gene="eno"
                     /locus_tag="K0W65_002773"
     CDS             complement(854..2152)
                     /gene="eno"
                     /locus_tag="K0W65_002773"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBI3095973.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2240..3877)
                     /gene="pyrG"
                     /locus_tag="K0W65_002774"
     CDS             complement(2240..3877)
                     /gene="pyrG"
                     /locus_tag="K0W65_002774"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBI3095974.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4105..4896)
                     /gene="mazG"
                     /locus_tag="K0W65_002775"
     CDS             complement(4105..4896)
                     /gene="mazG"
                     /locus_tag="K0W65_002775"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBI3095975.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4992..7226)
                     /locus_tag="K0W65_002776"
     CDS             complement(4992..7226)
                     /locus_tag="K0W65_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBI3095976.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7274..8575)
                     /gene="rlmD"
                     /locus_tag="K0W65_002777"
     CDS             complement(7274..8575)
                     /gene="rlmD"
                     /locus_tag="K0W65_002777"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBI3095977.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8632..11388
                     /gene="barA"
                     /locus_tag="K0W65_002778"
     CDS             8632..11388
                     /gene="barA"
                     /locus_tag="K0W65_002778"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBI3095978.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11620..12960)
                     /gene="gudD"
                     /locus_tag="K0W65_002779"
     CDS             complement(11620..12960)
                     /gene="gudD"
                     /locus_tag="K0W65_002779"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBI3095979.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12981..14321)
                     /locus_tag="K0W65_002780"
     CDS             complement(12981..14321)
                     /locus_tag="K0W65_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBI3095980.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14323..15675)
                     /gene="gudP"
                     /locus_tag="K0W65_002781"
     CDS             complement(14323..15675)
                     /gene="gudP"
                     /locus_tag="K0W65_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HBI3095981.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16109..16558)
                     /locus_tag="K0W65_002782"
     CDS             complement(16109..16558)
                     /locus_tag="K0W65_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBI3095982.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16576..17358)
                     /gene="truC"
                     /locus_tag="K0W65_002783"
     CDS             complement(16576..17358)
                     /gene="truC"
                     /locus_tag="K0W65_002783"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBI3095983.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17358..17687)
                     /locus_tag="K0W65_002784"
     CDS             complement(17358..17687)
                     /locus_tag="K0W65_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBI3095984.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18309..18854)
                     /gene="syd"
                     /locus_tag="K0W65_002785"
     CDS             complement(18309..18854)
                     /gene="syd"
                     /locus_tag="K0W65_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBI3095985.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18922..19770
                     /gene="queF"
                     /locus_tag="K0W65_002786"
     CDS             18922..19770
                     /gene="queF"
                     /locus_tag="K0W65_002786"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBI3095986.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19882..21246
                     /gene="ppnN"
                     /locus_tag="K0W65_002787"
                     /gene_synonym="ygdH"
     CDS             19882..21246
                     /gene="ppnN"
                     /locus_tag="K0W65_002787"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBI3095987.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5229 a         5670 c         5068 g         5289 t
ORIGIN      
        1 ttgttgcagc ccaaccaact attgtcccac aattacagca acaagttacg ggtcttacga
       61 gtagcttaaa ctcacaagaa aagaaagaac tgacgcacaa gttagaatct attttcaata
      121 atacccaagt gcaaattgct gtattaattg tttccacaac caaagatgag acaattgaac
      181 aatatgctac aagagttttt gacaattggc gtttaggaga tgccaaacgt aatgatggga
      241 tactgatcgt tgttgcctag tcggatcgca ctgtccgcat ccaggtaggt tatgggctgg
      301 aagaaaaggt aaccgatgct ctggctggag atatcatccg tagcaacatg atacccgcat
      361 ttaaacaaca aaaattagct aagggattag agttagctat aatcgctttg aataaccaac
      421 tcacttcaca acaccaatat ccggctaatc cttcagaaag tgaatcaacg tcttccactg
      481 atcattatta ctttgctatt ttttgggtat ttgcagtgat gttcttccct ttctggtttt
      541 ttcatcaagg cagtaatttt tgtcgcgcat gtaaaagtgg agtctgtatt tcagcaatcc
      601 atcttttaga tttattcctg ttctccgata aaactttttc cattgctgta ttttccttct
      661 tttttacttt caccatattt atggtcttta cctgtttatg tgtacttcag aaaagagcat
      721 ctggtagaag ttatcattca gacaacagcg gttccgctgg aggctcaagt ggcggcgcat
      781 ccggccgctg gtaactccac cataaaaaat gccagcccga aggctggcat tatcaaatca
      841 gataaagtca gacttatgcc tggcctttga tctctttacg accgttgtac ggtgcttttt
      901 cgcccagagc ttcttcgata cgaatcagct ggttgtattt agcaacacgg tcagaacggc
      961 tcatagaacc agttttgatc tggcctgcag cagtaccaac agccagatca gcgatggtag
     1021 catcttcagt ttcaccagaa cggtgagaga taactgcagt gtagccagca tctttcgcca
     1081 tcttgattgc agccagagtt tcggtcagag aaccgatctg gttgaatttg atcaggatgg
     1141 agttagcgat acctttttcg ataccttctt tcaggatctt ggtgttggtt acgaacaggt
     1201 cgtcaccaac cagctggatt ttgtcgccca gaactttggt ctggtatgcg aaaccgtccc
     1261 agtcagattc gtccagaccg tcttcgatag aaacgatcgg gtactgtttg gtcagttctt
     1321 ccaggaagtg agtgaattct tcagaggtga acgctttgtt gccttcgcca gccagaacgt
     1381 atttaccatc tttgtagaat tcagaagctg cgcagtccat cgccagagtg atgtctttgc
     1441 ccagttcata accagcagct ttaacagctt cagcgataac agccagagct tcagcgttgg
     1501 aacccaggtt cggcgcatag ccaccttcgt caccaacagc agtgttcatg cctttcgctt
     1561 tcagaacttt tgccaggtga tggaaaactt cagaacccat gcggatggct tctttcacag
     1621 ttttcgcgcc aaccggctga atcatgaatt cctggatatc aacgttgttg tcagcgtgct
     1681 caccaccgtt gatgatgttc atcatcggaa ccggcatgga gtatttgccc ggagtgccgt
     1741 tcagttcagc gatgtgctcg tacagcggca tacctttagc agctgcagca gctttggcgt
     1801 tagccagaga tacagccagg attgcgttcg cgccgaattt ggatttgttt tcggtgccgt
     1861 ccaggtcgat catgatcttg tcaatgccag cctgatcttt agcatctttg ccaatcagcg
     1921 cctgagcgat cgggccgttt accgcagcaa cagctttggt tacgccttta cccaggaaac
     1981 gggatttgtc gccatcgcgc agttccagag cttcacggga accagtagaa gcacctgacg
     2041 gagcagctgc cataccgacg aaaccaccct ccagatgtac ttcggcttca acagtcgggt
     2101 taccacggga gtcgatgatt tcacgaccga tgatttttac gattttggac attaggtttt
     2161 cctcaagtca ctagttaaac tgaaactcca gacaaacaac gcgtacccaa ggtacgcgtt
     2221 gccgttctaa cttttttact tacttcgcct gacgtttctg gaactcgctg gcggctttca
     2281 caaagcctgc aaacagcggg tgaccatcac gtggagtaga agtaaactcc ggatggaact
     2341 ggcaagccac gaaccacggg tgattcggaa cttcgatgat ctcgaccaac tgatcatccc
     2401 cggaacggcc cgcaacgcgc agacctgcat cttcaatctg tttcaacagc atgttgttga
     2461 cttcgtaacg gtgacgatga cgctcaacaa ttgtcggcgc attgtatagc tggcgaacca
     2521 ggctatcgtc aaccaactgg cactgctgtg cgccgagacg catggtaccg ccgagatcgc
     2581 tcttctcgct acgaacttca acgttgccgt tttcatcgcg ccactcggta atcagcgcca
     2641 caaccgggta cttacagtct ggcacaaatt ctgtagagtt ggcgttctcc atgttggcaa
     2701 catggcgagc gtaatcaatt aacgccacct gcatacccag gcaaatgccc agataaggaa
     2761 tattgttctc acgcgcaaaa cgcgcggtcg taatcatgcc ttctacgcca cgatagccga
     2821 aaccgccagg tacgaggatt gcgtccagac ctttaaggat ttcaacgccg cgcgtttcaa
     2881 catcttgtga atcgatcagt ttgatgttga cgctgacacg attcttcagc ccaccgtgtt
     2941 tcagtgcttc gatcactgat ttataagcat ccggcagttc aatgtacttg ccgaccatac
     3001 cgatggtgac ttcacttacc ggattcgctt cttcgaagat aacctgttcc cattcggaca
     3061 gatttgcttc cgggcagttt aagctgaatc gtttacaaat ataatcgtcc agcccctgag
     3121 atttcaacag gcccggaatt ttatagatgg aatcgacgtc tttcagagaa ataaccgctt
     3181 tttccggaac attacagaac aatgcaatct tcgcacgttc gttcgccggt acagcgcgat
     3241 ctgaacgaca aatcaggatg tcaggctgga taccgatgga gagcagctct tttacagagt
     3301 gctgagtcgg tttggttttg acttcaccag acgctgccat gtacggcacc agcgtcaggt
     3361 gcataaacag agtgtgctca cggccaattt caacagccat ctggcgaata gcttcgagga
     3421 acggcaagga ttcgatatca cctactgtac cgccgatttc taccagtact acgtcatgac
     3481 cttcgccacc ttccagcacg cgctctttga ttgcgttagt gatgtgcgga ataacctgca
     3541 cggttgcgcc gaggtagtca ccgcggcgtt ctttacgcag aacgtcagag tagatacgac
     3601 ccgtggtgaa gttgttgcgg cggctcattt tggtacgaat gaaacgctcg tagtgcccca
     3661 ggtccaagtc ggtttcagcg ccgtcttcag taacgaacac ttccccgtgt tggattgggc
     3721 tcatagtacc tggatcgacg ttgatgtaag gatccagttt catgatggtc acattgaggc
     3781 cacgggcttc aagaatggct gcgagggagg ctgcggcaat gcctttaccc agagaggata
     3841 cgaccccgcc ggtcacaaaa atatagttcg ttgtcatgct gaacctgaga agttaggttg
     3901 aaaagacgat ggaataacca agacgggaaa gcagtatacc cgaacatgac ctgtgccaca
     3961 aactttcatt atccctcctc ttcgccagcg cactattgaa atcaggagtg agaaaatagc
     4021 ccctttgggg taaatgtttt tgacgcaaat caagcgcttg tcatttaaaa aatgacacaa
     4081 atggcgcttg accgcgtaat tcccttagag atcaatttcc tgccgtttta cctgttgcca
     4141 gacttcttcc attgtttcga ggtcaacacc tgtcatttcc agtccacgcg cggcaacaat
     4201 acgctccact tcgcgaaaac gacgctcgaa tttttcgttc actttttgca atgcgatttc
     4261 tgctttcgtc cctaaatggc gagccagatt aaccgtggca aacagcaggt cccccatttc
     4321 ctcctccagt ttagcctggt cgacaacagc ctgccgcgcc tcgtacatca cctcgtcgat
     4381 ctcttcgtag actttatcaa ccaccgggcc aagcgtcgtc cagtcgaagc caacgttggc
     4441 gcaacgtttc tggatttttt gcgcccgcat taaagccggt aaactacgag gaatatcgtc
     4501 cagcgccgaa tgctgcgctt tctgcgcgcg ctcttcggtt ttgatttgct cccaacgggc
     4561 aagcacttca ctactgtttt cggcagaact atcagcaaaa acatgcggat ggcgacgctc
     4621 taatttatcg ctaatagcag cgcaaatatc attaaagtca aagcgccctt cttcctgagc
     4681 catttgcgcg taaaacacca cctggaatag cagatcgccc agttcaccgc gaagatcgtc
     4741 aaaatcttca cgggcgatgg cgtccagcac ttcgtaggtt tcttcaaggg tgtaaggcgc
     4801 aatggtggca aatgtctgct ctttatccca cgggcagccg ttttccggat cgcgcaggcg
     4861 ctgcataata gtgagcaaac ggtcgatttg attcattgaa ttgtcctgaa aattgcgggt
     4921 ctgtcaggtg taataccttc gagtaaaaga tatcgctgga tacggctgac gccatatcca
     4981 gctatgtaag actaactccc gtgcaaccga cgcgcgtcga taacatccgg cacctggttg
     5041 agtttaccca gcacgcgccc cagcacttgc aggttgtaaa tctcaatggt catgtcgatg
     5101 gtcgccagtt gctgtttggt gtcgctacgg ctggcaacgc caagcacgtt caccttctcg
     5161 ttggcgagaa tggtcgtgat atcacgtaac aacccactac gatcattagc caccacgcgg
     5221 accaccagcg aatatccggc ggagtagctc tcaccccata ccgcgtcaac aatgcgttct
     5281 ggcgcatggg agcgcagttc cgccagttgt tcgcaatcgg cgcggtgtac tgaaataccg
     5341 cgtccctggg taatgaagcc gacaatctca tctccaggaa tcggctggca gcagcgcgcg
     5401 atgtggtgca tcaggttacc aacaccttca accaccacac gaccgttatc tttactgcgg
     5461 ttttgcggtg tgtagctttt ttgctgaagc tgtttcagcg cggcggcgtc ctgctcttcg
     5521 gcactcggct tattaaattg cgattgcagg aagttcacca tctgattgag acggatatcc
     5581 ccgccaccaa tcgccgccag caactcgtcg acatcattga agttgtaacg cggcagcaga
     5641 tgtttttctg cttctttcag gctgatcccc agatgttcca gctcgtcgtc aaggatttgc
     5701 cgcccagcca gaatgttttt gtcacggtcc tgtttacgga accaggcgtg aattttcgaa
     5761 cgcccacggc tggttgtgac gtaaccgagg tttgggttta accagtcacg gctggggttc
     5821 ggctgtttct gggtgataat ttcaatctgg tcgcccattt gcagctggta ggtgaacggc
     5881 acaatgcgcc cgccaatttt tgccccgatg cagcggtgtc cgacatcact gtggatgtgg
     5941 taagcgaagt ccagcggcgt tgatcccgca ggcaaatcaa cgacatcacc tttcggcgta
     6001 aagacataca cccggtcgtc aaacacctga ctacgtactt cgtcgagcat ttcgccggaa
     6061 tcagccatct cttcctgcca cgcaatcagt ttacgcagcc aggcaatccg gtcttcatgt
     6121 cccgaacgtg cgccgccagc tgccgcgccc tctttatatt tccagtgcgc agcaacaccc
     6181 aactccgcat cttcatgcat ctgtttggtg cggatttgga tctcaacggt ttttccaccc
     6241 ggccccagaa ccacggtatg aatagactga taaccgtttg gtttcgggtt agcgacgtaa
     6301 tcgtcaaact catccggcag gtggcgatag tgagtgtgca ctatccccag tgcggcataa
     6361 caatcctgta aacgctcggc gacaatacgt accgcacgca catcaaacag ctcatcgaag
     6421 gcgaggttct ttttctgcat tttgcgccag atgctgtaga tgtgtttcgg acgaccatac
     6481 acttcagctt taacgccttc agctttcatc tcagcgcgca gatggccgac aaactcttcg
     6541 atatagtgtt cgcggtcgag acggcgttca tgcagcagtt ttgcgatgcg tttgtattcg
     6601 gtcgggtgga gataacggaa gcagtaatct tccagctccc atttcagttg cccaatccca
     6661 agacggttcg ccaacggcgc gtagatattg gtgcactctt ttgccgccag tacacgttca
     6721 tcttccggcg catcttttac ttcgcgcaga tgagcaatac gctccgccag tttgatgact
     6781 acgcagcgaa aatcatcgac catcgccaat aacatccggc gaacgttatc gacctgttcg
     6841 gaggaaacag aatcagtgtg cgtcgctttc agctggcgga tcgccgccat atcacgcacg
     6901 ccgtgaataa ggttaacaac cgacttaccg acgctctcac gcagcacatc ttcgctgact
     6961 acgttggcat cagccagagg gaacagcagc gccgcccgca gcgtgtcaat gtccatactt
     7021 aatgtcgaga ggatctccac catctcaaca ccacgccaca ataacagact ggcatccgga
     7081 tgcccctgcg tctgttgcag acaatacgcc caggtttcgg ctaagcactc acacgacttc
     7141 tggctggtaa tacccagact tgcgatccat ttttccggat caaattcacc agccttattg
     7201 atatgtgcac ttcttaccgc aaccatcgtc ctctccttta gggaccagac ctgccgaaat
     7261 cggcaaatcg taactattta acgcgcgaga aaagtaccat cgattccaga tgtcccgtgt
     7321 gtgggaacat atccagcatc gccagtcgcg caatggtata tcctgctttt aataacgctt
     7381 cgctatcccg agccagcgtt gcagggttac aggatacata aactatacga ataggttcca
     7441 gttttataat ttgctgcata acacctgcgg cacctgctcg cgccgggtcc agcaacactt
     7501 tatcgaagcc gtttttcgcc cacggctgct ttgtgacatc ttcttcaaga ttttcgtgat
     7561 aaaacgtcac attctgtaag ccattaagac gcgcattctg ctggcctttt tccaccagcg
     7621 caggaacacc ttctacaccg accacactgg cagcttgtgt cgccaatggc agtgtaaagt
     7681 tgcccatacc gcagaacaga tccagtacgc gatcttcagg ttgcacatcc agccattcca
     7741 acgcacgcgc taccattttt tggttcacac ccgcattgac ctgaataaaa tcgcgcgggc
     7801 taaaagttaa gcgcaacccg tttgagtcat accagggcat ctcaccagag acggtttcga
     7861 gtatctcact atcgggggcg agatacagat ccaggccttc agaatgcgaa aagcgttcca
     7921 gtttttcgcg atctgccgaa cttagcggtg cggtatggcg caaaatcatc agcgtgccgc
     7981 tggttgcctg taccagttca acatgaccaa gatggcgcat agcttgtaag ctgcccagac
     8041 atgccctgac tttgggcagc aatgcttcaa gttggggcgc taaaatgggg cattgtttga
     8101 cgtcgacaat gtcactggag cccgctttgc gaaaccccat ctgaagttgc tgtgttttcg
     8161 gtaagtagtt cagacttaaa cgcgcgcggc ggcgatagcc ccagggaaca tcggcgatca
     8221 cttcagagac atcgtgtttc attaatcggg cgagtgccgc acttttgctt cgctgctgta
     8281 aatccacgct ggcgtgttgt tgctgacagc cgccgcatac gccaaaatga ggacagcgtg
     8341 gcgtttcgcg ttccgggcta tcgcttaacc ggcgtacgac tttagcgcgg gcatactgtt
     8401 ttttatcttc agtaacagca acttccgcgt tttcctgcgg caataatccg gggataaata
     8461 acgttttgcc gttatgtcgc gccacgccct gaccaaaaga gtcgaggtcg ttgactgaaa
     8521 cggttatgat ctgacgcgtc gtcgtgcgtc gttttgcaga gtagaattgc gccattggcg
     8581 agactttctc aatttaacag tgtgacctta attgtcccat aacggaactc catgaccaac
     8641 tacagcctgc gcgcacgcat gatgattctg atcctggcac cgaccgtcct tattggttta
     8701 ttgctgagta tctttttcgt cgtgcatcgc tataacgact tgcagcgtca actggaagat
     8761 gccggtgcta gcattattga gccgcttgca gtttctactg aatatggcat gagcctgcaa
     8821 aatcgcgaat ctatcggtca gttaataagc gtactgcatc gtcgccattc cgatattgtt
     8881 cgcgcgattt cggtttatga tgaaaataac cgactctttg tcacctccaa ttttcatctt
     8941 gatccctcat caatgcagct cggcagcaac gtgccgtttc ctcgccagct cactgtcact
     9001 cgtgacggcg atattatgat cctccgcacg ccgattattt ctgagagtta ctcccccgac
     9061 gaatcgccca gtagcgatgc caaaaatagt caaaatatgt tgggatatat tgcgcttgag
     9121 ctggatctta aatcggttcg cttgcagcaa tataaagaga tctttatttc ctgcgtgatg
     9181 atgctgtttt gtatcggtat tgcgcttatt tttggctggc gcttaatgcg cgatgtaacc
     9241 ggtccgattc gcaacatggt gaataccgtc gaccgcatcc gtcgcgggca actcgacagc
     9301 cgggtggaag gatttatgct cggcgagctg gatatgctga aaaacggtat caactcgatg
     9361 gcaatgtcgc tggctgctta tcacgaagag atgcagcaca atatcgacca ggcgacgtcc
     9421 gatctgcgtg aaacgctgga gcagatggaa attcagaacg ttgagttgga tctggcgaaa
     9481 aagcgcgccc aggaagcggc gcgtattaaa tccgagtttc tggcaaatat gtcacacgag
     9541 ctgcgtacac cactgaatgg tgttattggc tttacccgcc tgacgctgaa aacagaatta
     9601 acaccaacgc agcgcgatca cctgaatacg attgaacgtt cggcaaataa tttgctggca
     9661 attattaatg atgttctcga cttctcgaaa ctggaagcag gtaagctgat tctggaaagt
     9721 attccattcc cactacgcag cacgctggat gaagtcgtta ctctgctggc acattcttct
     9781 cacgataaag ggttggaact gacgctcaat attaaaagcg acgtgcctga taacgtgatc
     9841 ggcgacccac tgcgattaca gcaaatcatc actaacctgg tggggaatgc aattaaattc
     9901 accgagaatg gcaacattga tattctggta gaaaaacgtg cgctgagtaa taccaaagtg
     9961 cagattgaag tgcagattcg ggataccggc attggtattc ctgaacgcga tcaatcgcgc
    10021 ttattccagg ccttccgaca ggctgatgcc agtatttccc gccgtcatgg tggcaccggg
    10081 ctggggctgg tgattacaca aaaactggtt aatgaaatgg gcggcgatat ttcgttccat
    10141 agccagccga atcgcggttc aactttctgg ttccacatta atctcgatct gaacccgaac
    10201 attattatcg aagggccatc catccagtgc ctcgcaggta agcgcctggc ctatgtcgaa
    10261 ccaaactccg cagcagcgca atgcacgctg gatattttaa gtgaaacgcc gctggaagtg
    10321 gtttatagcc caacgttctc cgcgctgcct cccgcgcatt acgacatgat gttgttaggc
    10381 atcgcggtga ccttccgcga gccgctaaca atgcaacatg agcgattagc gaaagcggta
    10441 tcgatgaccg atttcctgat gctggcactt ccttgccatg cacaagttaa tgctgaaaaa
    10501 ctcaagcaag atggtatcgg cgcgtgtctg ctgaaaccat taacacctac gcgcctgttg
    10561 cctgccctga cggaattttg tcatcacaaa caaaacacgc ttttgcctgt aaccgatgaa
    10621 agtaagctgg caatgacagt catggcggtt gatgacaacc ccgctaacct gaaacttatc
    10681 ggcgcattgc tggaagatat ggtgcaacat gtggaacttt gcgatagcgg gcatcaggca
    10741 gttgaacggg cgaaacagat gccgttcgat ttgatcttaa tggatattca aatgccggac
    10801 atggatggca ttcgggcctg cgaactcatc caccaactcc cgcatcagcg acaaacgccg
    10861 gttatcgcgg taacggcgca tgcaatggcc gggcaaaaag agaagctgct tggcgcaggg
    10921 atgagcgatt atctggcgaa accgattgaa gaagagcgat tgcataattt gttgttgcgc
    10981 tacaagcctg gcagcggtat ttcgtctcgc gtcgtgacgc ccgaagtcaa cgaaattgtg
    11041 gtgaacccga atgcgaccct cgactggcaa ctggcactac gccaggcagc aggaaaaacc
    11101 gatttagcgc gcgatatgct gcaaatgtta ctcgatttcc tgcctgaagt tcgcaacaaa
    11161 gttgaggaac agctggctgg agaaaacccg gaaggcctgg ttgatttgat tcataaactg
    11221 catggcagtt gcggctatag cggcgtacca cgtatgaaga atctctgcca gctgatcgaa
    11281 caacagctac gtagcggtac taaagaagaa gatttggagc cggagctgct ggaactgttg
    11341 gacgagatgg ataatgtcgc gcgcgaagcc agcaaaattc tcgggtaacg gacgtcttgt
    11401 cgagtagcaa tcggcttttt atgacgctgg tttcagattg gtgacaaagt gcctgtgttt
    11461 atgccggatg cggcgttaac gccttatccg gcctacaaaa ccaattaaat tcaatgaatt
    11521 aaaaaatgat gtagacctga tacatagcac aacaggctat tttgcgttta gcatcagtct
    11581 caaaccggct ccagatagag ccggttttgg ttttctgtct taacgcacca tgcacgggcg
    11641 cttgttatcg aacgtccagc caggaatcag atactgcatt cccatcgcat cgtcacgcgc
    11701 gccaagcccg tgtttctgat acagctcatg ggctttcatc acttgatcca tatcgatttc
    11761 tacacccagc cccggttttt ctggcacctg taccagcccg cctttgatct caaacggttc
    11821 tttggtcagg cgctgattac cttcctgcca gatccagtgc gtatcaatag cagtaatttt
    11881 acccggtgca gcggcggcaa catgggtaaa catcgccagg gaaatatcga agtggttgtt
    11941 tgagtgtgaa ccccaggtca ggccaaattc atggcacatt tgcgccacac gtaccgaacc
    12001 ttgcatagtc cagaaatgcg gatccgccag cgggatatca acggattgta acgagagcgt
    12061 atggcccatc tgccgccagt cggtggcgat catattggtt gcagtcggca ggccagtcgc
    12121 gcgacggaac tctgccatca cttcacgccc ggagaaacct tgctccgcac cacacggatc
    12181 ttctgcataa gccagcgaac ctttcaggta tttaccaatt ttaattgctt cgttcagcga
    12241 ccaggcaccg ttaggatcga gcgtaatacg cgcctgcggg aagcgttgcg ccagtgccac
    12301 aatagactcg gcttcttctt ccccggccag tacaccgcct ttcagtttga aatcgttgaa
    12361 gccatatttt tcatacgccg cttccgccag gcgcaccacc gcatccggcg tcatcgcttc
    12421 ttcatgacgc aggcgatacc agtcgcatga gtcatccggc tggctttgat acggcagagg
    12481 tgtggctttg cgattaccga cgaagaacag ataaccgagc atttcgactt cgctacgctg
    12541 ttgaccatcg cccagcagcg atgccacgtt tacccccaga tgctgcccca gcagatccag
    12601 cattgccgct tctatcccgg taactacatg aatagtggta cgcaaatcaa atgtctgcaa
    12661 accgcgccca ctagcatcac gatcggcaaa agtattacgc accagcgtca gaacgttttt
    12721 gtattcaccc agcgttttac ctaccaccag cggaatcgca tcttccagcg ttttacggat
    12781 tttctcgccg ccgggaattt cccctacgcc agtgtgaccg gaattatctt tgataatcac
    12841 aatattacgc gtgaagaacg gtgcgtgtgc accactcaga ttcatcagca tactgtcatg
    12901 acccgccacc gggatgacct gcatttcagt aacaacaggc gtcgtaaatt gagaactcat
    12961 atttatgtcc tttttcagaa tcaatgacgg ccgaaaacgg gacgtttacg gtcaaaggtc
    13021 cagccgggga tcaggtactg catcggacct gcgtcgttac gcgcaccgca aggcagacgt
    13081 ttataggcct catgtgcctt ttgtacctgt tcccagtcca gttccacgcc cagaccgggc
    13141 gcatcaggaa cggcaatttt tccgttttta atctccagcg gatttttggt caggcgacaa
    13201 tcgccctcct gccaaatcca gtgggtatcg atagcggtag gattacccgg tgccgccgcg
    13261 cccacatggg taaatatcgc cagagagata tcgaaatggt tgttggaatg gcagccccag
    13321 gtcagccccc agtcgtcgca aagctgcgcc acgcggactg caccggaaag cgtccagaag
    13381 tgcggatcgg caagtggaat atctaccgca ttgagcatca ccgcatgacc catttcgcgc
    13441 cagttggtgg cgatcatgtt agtcgcgacg ggcaagccgg tcgcccgtcg aaactccgcc
    13501 atcacttcac gtccggagaa gccctgttct gcgccgcatg gatcttcggc ataggtaaga
    13561 acatcattca gccctttgca taaagaaatg gcttcatcaa gcagccatgc accgttggga
    13621 tcaacggtaa tccgcgcatc cgggaagctt ttcttcaatg cacgaacagt gtcgatttct
    13681 tgctcgccag gtaacacccc gcccttaagt ttgaaatctt taaagccata gcgatcctgt
    13741 gaggcttccg ccagacgcac aacggcttcg ctgttcatcg ctttctgatg gcgcaactga
    13801 taccactcat ggttgcccgg cgtattttcc agataaggaa gatcggtttt ggtccgatca
    13861 cctatataaa acagataacc gaggacggta atagtctcgc gttgcttgcc tggccccaac
    13921 agttcgcaga ccggaacatt cagcgcctta cctagcaggt caagcaaggc ggcttccagc
    13981 gccgccacgg cattaacgcg caattcaaaa gtccaggcac ctttgccaaa ggtatcaaaa
    14041 tcggctgcct gattaccttt atgtacctgc tgaaccactt tattcaggcg cgcaacttcc
    14101 tggcccagaa ccatcggaat agcatcgacc agtgtttgat aaatcacctc tccgcccggc
    14161 gcttcaccaa tgccggtatg cccggcgtta tcggtgagta ccacgatatt gcgggtgaag
    14221 tatgcgttat gcgcaccacc aatattgagc aacatgctgt catgtccggc gaccggaatg
    14281 actttcatat cagtaataac aggactggat tgtgtcgcca tcttattgcc ccgcgacagg
    14341 tttcagctcg atacgcttga tatcgcccac cagcaccagg tagctcagta ccgcgattag
    14401 ggcatgaaca ccaacataaa tcagcgcccc attaaacgag ccagtcgtgc caacgatata
    14461 accaattgcg attggcgtga cgatgccaga aatgttaccg aacatgttga acaggccacc
    14521 ggaaagaccg ctgatctctt ttggcgcggt atctgccatt actgcccaac ccagcgcccc
    14581 gatgccctta ccgaagaagg ccagcgccat aaagccgatg atcatccact caacgttgac
    14641 gtagttgcag aacaccatca ccatcgacag caacatgccc attacgatcg gtgttttacg
    14701 cgcaatgttc agcgatcccg tgcggcgcat cagccagtcg gaaataattc cacccagcac
    14761 accgccgata aaaccgcaaa ccgccggaac ggaagccaca aagcccgctt tcagaatcga
    14821 catcccacgc gcctgcacca gataaaccgg aaaccaggta atgaaaaagt aagtcagggc
    14881 gttgatacag tactgaccga tataaacgcc gatcatcatc cgtgacccga gcagctgttt
    14941 gatctgcccc cacttcacgc tgaacggcac tttaactttg gtgctttgct gatccatatt
    15001 gatcagcgcg ccacccgcgg cgatatactc cagctctttc tgatttaccc ccggatgttg
    15061 atttggctcg tggatgactt tcaaccagat aaagctgatg acaatgccca gaccgcccat
    15121 aaagaagaag acgtgtgacc agcccacttc atgcgtcagc cagcccataa tcggcgcgaa
    15181 gatcaccgtt gcgaagtatt gagcggagtt aaaaatcgac accgccgttc ccctttcctg
    15241 caccggaaac caggccgcaa caatgcgact gttgccgggg aaagatggcg cttcagcaag
    15301 cccgaccagg aagcgcagag taaacagggc aacgataatg ccgaatccac taaagatatc
    15361 gacgaagcct tgcagcaagg taaacatcga ccagataaag atcgaccaga agtagacgcg
    15421 ttttgaacca aaacgatcca gcaaccaacc accagggatc tgcccgataa cataagccca
    15481 tgagaaggca gagaacacat agcccattcc cacgggatca aggccgatat ctttggccat
    15541 ttccgaaccg gcaatagaaa gcgtagcgcg gtcgccgtag ttgaaggatg tgacgataaa
    15601 caacatcacc actatccagt aacgagcatt cgtgcgtttt tccacactgc tcgcagcctg
    15661 acttaaagaa ctcattgttg cactcctgaa aattcgcgtt agccacgctc actctggact
    15721 gcgacatcgc caggaaatca gaggtgacgt agggtgtttt ttgccgtttt tataggtcgt
    15781 tcgccgaata cggcgcgtgt ttatatctgg caatagcagt ataaaaagcg cgccatagcg
    15841 gctcaccgtg caacaacaca acattaatgc gttcaatgag gcccgatttt ggcattagcc
    15901 ctggacggtg gaatccactt cacggaaatg aaaacaagaa caagaaagga agggtaaaaa
    15961 cgaagaaata aagaagagta tgaaatggat cgcttgactc taggcaaacg ccagtaaaaa
    16021 tccgcgctat gaagcagttt ttactggcat ttgcctgaaa agattcgatt cagcaccgct
    16081 aaaacgacat ttaccgctcg ctgacatatc aggacaacag cgtgccccac tgttcgaccc
    16141 acggatttga ttccgtttcc ggttccgggt tctcactggc gtcaatcaac agcatttcgc
    16201 caacccgctg agcgctctgt tcctgcaaca aggcatcgaa ctgtttgccg ccattgcaaa
    16261 aatttacata actgctgtcg ccgagcgcaa tcacgccata acgcagattc ggctggaagc
    16321 ccagactatc tttgattccc tgaaagagcg gcacaatgct atcaggaagg tcgccctgcc
    16381 cggtcgtgga cgtaaccacc agaacatact tatcctgata gggcagccag tcgcttaatt
    16441 caggatcttc aaataccgtt gctttgtggc cctgtgcggt cagaattgct tctgcttctt
    16501 ccgccactaa cagcgaattt ccgtacatgg tgccgacaaa aataccaatt tccgccatac
    16561 cgttttctcc ctggattagg aacttatctc tccatcctga cccgatggcg cactgaactc
    16621 aaccctttca ttttcaggaa gcagaccgcg ccagccaaat tgtgataacg cctgcatcca
    16681 ggtgtcgtcc aggcccgcgt gaatagtcag cggctcgcca gtaaaaggat gcgtcaatga
    16741 caactgactg gcatgtagca ttaaccgctg gaggccaaaa tgctcagcac cgctgcgatt
    16801 ctggcgtaaa tcgccatgtt tgctatcgcc aataatcgga tgacgcaaat gggcaagatg
    16861 tcgtcgaagc tgatgtttgc gtccggtttt cggctccagt tccaccaggc cgtagcgcgt
    16921 ggtcgggtaa cgtccggtcg ctaccggcat ttctacggtc gccagaccgc gataatgcgt
    16981 cactgctggc tgcgggcctt tatcttcgcg ggcaaattta tcagcgattt tgtccagttc
    17041 ttccaccagt ggataatcca acaccgcttc ttccatcaac cagccgcgca caatcgcatg
    17101 gtaacgtttc tggatttggt gctgttcaaa ctgttgtgcc agcagccgtc cggcctcgct
    17161 ggataatccc atcaacaaca caccagaagt gggtcggtcc agacgatgag cagtaaaaac
    17221 atgctgtcct atctggtcac gcacggtttg catgaccact actttctcgt cgcgatccag
    17281 ccagctacgg tgaaccagcc agccggaggg tttatttacc gcaaccagcc attcatcctg
    17341 atagagtatt tccagcatta gctcgcatca tccgcaaaaa gagcatccag tttttccaac
    17401 tcagccagaa taagcgcgcg ttgcggatgg tccgtcgcca gcgccatttc ataatagggt
    17461 gcaacagcaa aagcgcccgg taacggctgg ttgttattta acaaatcgtg cattcgcggg
    17521 atcagccccc actgcaacca ctccagtggt tccatggtgt ccataaagaa cggttgggta
    17581 ctattaaatt gatgcggctg gggttcatcg tttcgccagt gctgatgttc acgcagtaac
    17641 gcttcaagcg cctgcaactg gagacgaacg cggtcatgag tggtcataaa gcaacctcaa
    17701 taagaaaaac tgccgcgaag gatagcagga ataaaaaaag ggagcactgt attcacagtg
    17761 ctcccggttc gtttcgcagc attccagcta cttttgttgc tccctgctca tccttgacaa
    17821 cttttcctct ggccttgcgg ccaatcgttc atcctgaact attgcttcct gctcacacca
    17881 ccccgatgtg atacttcatc ctgaagtgtc cctggccttc ctgacccacc gaatcatcct
    17941 gaccggttct cattctccat cctggaggtg tcctttaacg cgtcctgcgt catcctcttc
    18001 gcttcatcca gaagcctttc cctgaaacac catcctggtg tgtcctgcag aagtgtcatc
    18061 atcctgatgt tcacttcgtt gtctgactcc ctgtcgacga agatagaatc gtctttttcg
    18121 cgaagtctta caaggcgctt acagacaatg ccttaaggaa aatttcatat gaaagttaag
    18181 aattaacaat caacattttg attttaaatg aaaataacga ttcttatctt tttaagtatt
    18241 cgcttatttc ctggcgatct cctacagagt ctgtaagaga tctctcacaa ccagatgggg
    18301 tatttggatt acagaagagg cttaagttga ttaaggaatt ccgcaagatt tgaggctaaa
    18361 ttcgtccgtt tacgtgttcc cagcgtctct ttgcacactt caccggagag attacacacc
    18421 gagatgacct ctaactcttc ttccagcgtc gcgataaata gcgtgggcgg cagttttagc
    18481 cttttctgag tcaccagatg accaatcaga ttttcctgca cccgacggaa gtcgtcttca
    18541 ctccaggttt gcagcaatgt cagtttgata tcgccaaact gcgcgtgcat atccccggca
    18601 aactgagtcg tataaaaggt atgaattgtg ggttgtatca caatatcaaa agcgcgttca
    18661 accgcgttta cattttgttc cccggtaaac ggctggggtt gccagtatac ggcatcttcg
    18721 gtagttgaaa taatgcacgg tgaaggaaca ccgtacaatt cctcacttaa cggccagctt
    18781 ttatgctctt catgccacgc atcgcagtag cgtgcagtaa aatctttcag ggcttgtgcg
    18841 gtcaaatcgt ccactaattt ctctcttcac ttaaaccaga tacacttgtc gtttagttta
    18901 tctggtttat gacggtgaaa catgtcttct tatgcaaacc atcaggcact tgcgggcctg
    18961 actcttggaa aatcaaccga ttaccgggat acctatgacg ccagcctact gcaaggcgtt
    19021 ccacgcagcc tgaatcgcga cccgctgggt ctgaaagcgg ataacctgcc ttttcacggt
    19081 acggatatct ggacgctgta tgaactttcc tggctgaatg cgaaaggttt gccgcaggtc
    19141 gctgtcggtc atgttgaact tgattacacc agcgtaaatc tgattgagtc gaagagtttt
    19201 aagctctatc tcaacagttt taaccagacg cgttttaata actgggatga ggtgcgccag
    19261 acgctggagc gcgacttaag cacttgcgct cagggtaaga ttagcgtggc gttatatcgt
    19321 cttgatgaac tggaaggcca gccgataggt catttcaatg gcacttgcat tgatgaccag
    19381 gatatcacta tcgataacta tgaattcact actgactatc tggagaatgc caccagcggt
    19441 gaaaaagtag tggaagagac gctcgtcagc cacctgctga aatcaaactg cctgatcacc
    19501 catcaaccag attggggttc gatccaaatt cagtatcgtg gacgccaaat tgacagagaa
    19561 aaactgctgc gttacctggt ctcattccgt catcacaacg agttccacga acagtgcgtg
    19621 gaacgcatct ttaatgacct gttacgcttc tgccagccag agaaattgag cgtttacgca
    19681 cgttataccc gtcgtggcgg tctggacatt aacccgtggc gcagtaatag cgattttgtc
    19741 ccatcgacca caagactggt tcggcaataa attttttctc aattttgcgt gctggattca
    19801 cgcagaaggt tgtgaaaggt catcaggcag ggctattgta atcaaaggga atgacgatat
    19861 tcgtcccata aggagttttt cttgattaca catattagcc cgcttggctc catggatatg
    19921 ttgtcgcagc tggaagtgga tatgcttaaa cgcaccgcca gcagcgacct ctatcaactg
    19981 tttcgcaact gttcacttgc cgtactgaac tccggtagtt tgaccgataa cagcaaagaa
    20041 ttgctgtctc gttttgaaaa tttcgatatt aacgtcctgc gccgtgaacg cggcgtaaag
    20101 ctggaactga ttaatccccc ggaagaggct tttgtcgatg ggcgaattat tcgcgctttg
    20161 caggccaact tgttcgcggt tctgcgagac attctcttcg tttacgggca aatccataat
    20221 accgttcgtt ttcccaacct gaatctcgac aactccgtcc acatcactaa cctggtcttt
    20281 tccatcttgc gtaacgctcg cgcgctgcat gtgggtgaag cgccaaatat ggtggtctgc
    20341 tggggcggtc actcaattaa cgaaaacgag tatttgtatg cccgtcgcgt cgggaaccag
    20401 ctgggcctgc gtgagctgaa tatctgcacc ggctgtggtc caggagcgat ggaagcgccg
    20461 atgaaaggtg ctgcggtcgg acacgcgcag cagcgttaca aagacagtcg ttttattggt
    20521 atgacagagc cgtcgattat cgccgctgaa ccgcctaacc cactggtcaa cgaattgatc
    20581 atcatgccgg atatcgaaaa acgtctggaa gcgtttgtcc gtatcgctca cggcatcatt
    20641 atcttccctg gcggtgtggg tacggcagaa gagttgctgt atttgctggg aattttaatg
    20701 aacccggcca acaaagatca ggttttacca ttgatcctca ccggcccgaa agagagcgcc
    20761 gactacttcc gcgtactgga cgagtttgtc gtacatacgc tgggcgaaaa cgcgcgccgc
    20821 cattaccgca tcatcattga tgacgccgct gaagtcgctc gtcagatgaa aaaatcgatg
    20881 ccgctggtga aagaaaatcg ccgtgataca ggcgatgcct acagctttaa ctggtcaatg
    20941 cgcattgcgc cagatttgca aatgccgttt gagccgtctc acgagaatat ggctaatctg
    21001 aagctttacc cggatcaacc tgttgaagtg ctggctgccg acctgcgccg tgcgttctcc
    21061 ggtattgtgg cgggtaacgt aaaagaagtc ggtattcgcg ccattgaaga gtttggtcct
    21121 tacaaaatca acggcgataa agagattatg cgtcgtatgg acgacctgct acagggtttt
    21181 gttgcccagc atcgtatgaa gttgccaggc tcagcctaca tcccttgcta cgaaatctgc
    21241 acgtaatctc ccctcc
//
LOCUS       DAETQL010000068        20862 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.068, whole genome shotgun sequence.
ACCESSION   DAETQL010000068 DAETQL010000000
VERSION     DAETQL010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.068"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..74)
                     /locus_tag="K0W65_002788"
     CDS             complement(<1..74)
                     /locus_tag="K0W65_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HBI3095988.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQLV"
     gene            complement(172..1281)
                     /gene="anmK"
                     /locus_tag="K0W65_002789"
     CDS             complement(172..1281)
                     /gene="anmK"
                     /locus_tag="K0W65_002789"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBI3095989.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1555..2022
                     /gene="slyB"
                     /locus_tag="K0W65_002790"
     CDS             1555..2022
                     /gene="slyB"
                     /locus_tag="K0W65_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBI3095990.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="K0W65_002791"
     CDS             complement(2069..2503)
                     /gene="slyA"
                     /locus_tag="K0W65_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBI3095991.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2704..2940
                     /locus_tag="K0W65_002792"
     CDS             2704..2940
                     /locus_tag="K0W65_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBI3095992.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2943..3800
                     /locus_tag="K0W65_002793"
     CDS             2943..3800
                     /locus_tag="K0W65_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBI3095993.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3800..5812
                     /locus_tag="K0W65_002794"
                     /pseudo
     CDS             3800..5812
                     /locus_tag="K0W65_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="K0W65_002795"
     CDS             complement(5813..6334)
                     /gene="sodC"
                     /locus_tag="K0W65_002795"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBI3095994.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6415..7311)
                     /locus_tag="K0W65_002796"
     CDS             complement(6415..7311)
                     /locus_tag="K0W65_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBI3095995.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7360..7599)
                     /locus_tag="K0W65_002797"
     CDS             complement(7360..7599)
                     /locus_tag="K0W65_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBI3095996.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7702..8301
                     /gene="nemR"
                     /locus_tag="K0W65_002798"
     CDS             7702..8301
                     /gene="nemR"
                     /locus_tag="K0W65_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HBI3095997.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8338..9435
                     /gene="nemA"
                     /locus_tag="K0W65_002799"
     CDS             8338..9435
                     /gene="nemA"
                     /locus_tag="K0W65_002799"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBI3095998.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9516..9923
                     /gene="gloA"
                     /locus_tag="K0W65_002800"
     CDS             9516..9923
                     /gene="gloA"
                     /locus_tag="K0W65_002800"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBI3095999.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10026..10673
                     /gene="rnt"
                     /locus_tag="K0W65_002801"
     CDS             10026..10673
                     /gene="rnt"
                     /locus_tag="K0W65_002801"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBI3096000.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10766..12880
                     /locus_tag="K0W65_002802"
     CDS             10766..12880
                     /locus_tag="K0W65_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBI3096001.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12890..14182
                     /locus_tag="K0W65_002803"
     CDS             12890..14182
                     /locus_tag="K0W65_002803"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096002.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            15132..15224
                     /locus_tag="K0W65_002804"
     CDS             15132..15224
                     /locus_tag="K0W65_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBI3096003.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(15268..16476)
                     /locus_tag="K0W65_002805"
     CDS             complement(15268..16476)
                     /locus_tag="K0W65_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3096004.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16484..16984
                     /gene="tnpA"
                     /locus_tag="K0W65_002806"
     CDS             16484..16984
                     /gene="tnpA"
                     /locus_tag="K0W65_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBI3096005.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(17023..17370)
                     /gene="grxD"
                     /locus_tag="K0W65_002807"
     CDS             complement(17023..17370)
                     /gene="grxD"
                     /locus_tag="K0W65_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBI3096006.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17705..18532
                     /gene="mepH"
                     /locus_tag="K0W65_002808"
     CDS             17705..18532
                     /gene="mepH"
                     /locus_tag="K0W65_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HBI3096007.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18660..19241
                     /gene="sodB"
                     /locus_tag="K0W65_002809"
     CDS             18660..19241
                     /gene="sodB"
                     /locus_tag="K0W65_002809"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBI3096008.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19387..20556)
                     /locus_tag="K0W65_002810"
     CDS             complement(19387..20556)
                     /locus_tag="K0W65_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3096009.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20722..20811)
                     /gene="ynhF"
                     /locus_tag="K0W65_002811"
     CDS             complement(20722..20811)
                     /gene="ynhF"
                     /locus_tag="K0W65_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBI3096010.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
BASE COUNT         4980 a         5490 c         5303 g         5089 t
ORIGIN      
        1 accagttgat taaaggcgct acaaccggag agaaggaccg gcaaaatgat aattaacagt
       61 tttttcatag tcatatcccg aagactttcc tggtccggag ggcaaatacc ctccttaatc
      121 ttccaagtgt aacggcagac acgataagaa aaaattcagt taattctgat atcacgagtt
      181 ggcggggaaa atagccccca gtaccgtctc ctggcttgcg ccagtgacgg aaggcagatt
      241 tcctggtaat cccgccagcg tccgccaggc aagccaggcg aaagccaatg cttccatgtc
      301 atcgccacta atgccaacgg catcggtggt ggtgacttct gtgcctagca gtaataccgc
      361 cagacgcgcc atgagtagcg gattacgact acctccacca cataccatca atcgttcgca
      421 gccaccgctc aacaaaactt gttcagaaat ggtcacggcg gtgagttctg ccagtgtggc
      481 ctgaacatca cggggatcaa cacccggaaa atggcgtaaa tggcgctcca gccagccgta
      541 gttaaagtat tcgcgtccgg tgcttttcgg tgcaggttgc gagaaatacg ggtcgctgag
      601 catattttgc agcagtggga gaataacttt acccgcccgt gcccactcgg catctttatc
      661 gtaaggttta cctgcctgac gccagatcca ggcatccatc agcatgttac caggaccggt
      721 atcgtagccg ccaaccagct gcccaggaat gagcagtgac agattggcga tgccgccaat
      781 attgagcacc attcgtcgct cggttgggtg agccagcaag gcatgatgga acgcaggtac
      841 cagcggtgcg ccttgtcctc ccaaggcaat atcgcgacgg cgaaaatcac cgacaaccgt
      901 aattccggtg cgtgccacaa tttgattgtt atcgccaatc tgtaaagtgt gtggtgccac
      961 gcccgtcggt tcatgccaga cggtttgacc gtgacagccg atcgcaacta tatctctcgc
     1021 ctgcaggttt tgttccttaa gcaaggcatt gaccgcatca gcaaaaagtc gcccgagttg
     1081 agtatcaagc tgtccaaact gcgaaagtgt aagctgctgg ccctggcaaa tatccagtac
     1141 agcctgtttc agagacaccg ggatcggcca gctcaagctt gccagctgtg cgacccggtg
     1201 ttcatcaatt gtcgccaaca caacatcaac accatcaagg ctggtgcctg acataacgcc
     1261 aataaagcgg cccgatttca tagttcatcc tttttcaatc tgacgtttgc gcaccactca
     1321 aacataaact tttcgtgaat accatgcgga atgaccgatt tttaccgttg gtagtaaaac
     1381 attatcttca aatcaataat catcatgaat gttttgttta taattggttg atcctacttt
     1441 cattatgatt tgctcatatt tggtagaaca tgtaaccatg gattcacata tgccatatac
     1501 tttgaccatg agggatgctt gcgtggcgtt tcatggtgaa caggagattt ttcaatgatt
     1561 aaacgcgtat tggttgtttc aatggtaggt ctgtctcttg tcggttgtgt taataacgac
     1621 accctgtcag gggatgttta taccgcttct gaagcgaaac aagtacagaa tgtcagctat
     1681 ggcaccatcg ttaacgtacg tccggtacag attcagggcg gtgatgattc caacgttatc
     1741 ggtgcaattg gcggtgctgt tcttggtggt ttcctgggga atactgttgg tggcggaacc
     1801 gggcgttctc tggctactgc agcaggcgct gttgcaggtg gcgtagctgg tcagggcgta
     1861 cagagtgcaa tgaacaaaac gcagggtgtc gagctggaaa ttcgtaaaga cgatggtaat
     1921 accatcatgg tggtacagaa acaaggcaac actcgtttct ctccgggcca acgtgtcgta
     1981 ctggccagca atggcagtca ggtgaccgtt tctccgcgct aaataagttt gcgtgtggtc
     2041 aggttactga ccacacgccc ccttcatttc accctttggc ctgtaactca atgatattat
     2101 gctcaagttt tgcgatgagc gtaatcagtt gctccagttc ctctgcggag atgccatgta
     2161 atatttccgc gcgggttttg ttaataacag cttccatttc gctgatcagt ggctctgcct
     2221 tttccgtcag tttaatacgt ttagcccgac gatcgctggc acaagtttga cgcgaaatta
     2281 accctttttc ttccagttgg tccagagtac ggaccagtga tggctgctcg atgccaatcg
     2341 cttttgccag ttgaatttgc gactggtctg gaggtaactg atggatattg tgtaacgtaa
     2401 cccaatgggt ttgtgttaac tccagcggtt tcaggcgatg gtctatcaga gcacgccata
     2461 tgcgcaccaa ccgtgccaga tcagaaccta gtggcgattc caatttcatc tccttataat
     2521 tagcttgcta agatattatg cggcttttag aatagtgtgc agcaattgta ttgctaaaac
     2581 aaatgtattg ctgcatttgg ttaccgtcag acatattttt cagaaattgc gcgtaaattt
     2641 ttcgcactta aagaatattt attaatctaa cgcaatatat tcggtcgtaa aggaatctac
     2701 tttgtgaagt ttatgctcaa tgcaacagga ttgcccttac aagatctggt gttcggtgcg
     2761 tccgtctatt ttcctccgtt tttcaaagca ttcgcgtttg gattcgtcat ctggcttgtc
     2821 gtacaccgcc tgcttcgtgg ctggatctac gccggcgata tctggcatcc cttgttaatg
     2881 gatttatcgc tgtttgcgat ttgcgtttgc cttgctctgg caatactgat tgcgtggtaa
     2941 ctatgtcaat taaaacaatt aagtatttct caacaatcat tgtagcgata gttgcggttc
     3001 ttgccggatg gtggctgtgg aattattaca tgcaatctcc gtggacacgc gacgggaaaa
     3061 tacgcgcaga acaggtttct atcaccccac aggtgtcggg acgtattgtt gagctgaata
     3121 taaaagacaa ccaactggtt aacgcggggg aacttctgct cacgatcgat aaaacgccct
     3181 ttcagatcgc cgagctgaac gcacaggctc agctggcgaa agcgcaatct gacctcgcaa
     3241 aagccaataa cgaagcgaat cgtcgtcgtc atctctcaca aaattttatt tttgccgaag
     3301 aactcgatac cgctaacctc aatgttaaag cgatgcaggc cagtgttaat gccgcacagg
     3361 cgacgctgaa acagacacaa tggcaactgg cgcaaacgga aattcgcgcc ccggtgagtg
     3421 gatgggtgac taacctcact acccgcatcg gcgattacgc tgacaccggg aaaccgctgt
     3481 ttgcccttgt cgacagccac tcgttttacg tcattggtta ttttgaagaa accaagttgc
     3541 gccatatccg cgaaggtgca ccagcacaaa ttaccctcta tagtgataac aaaacgttac
     3601 agggtcacgt ttccagtatc ggtcgggcga tttatgatca gagcgttgaa agtgactcaa
     3661 gccttattcc ggatgtgaaa cctaacgtcc cctgggttcg tctcgcccaa ctcgttcccg
     3721 tccgctttgc gctggataaa gttccaggcg atgtcacgct ggtgtcgggc accacctgta
     3781 gcattgccgt aggtcaataa tgaacgcatc gtcatggtcc ttgcgcaatt tgccctggtt
     3841 cagggccacg ctggcgcaat ggcgttatgc gttacgcaat accattgcca tgtgtctggc
     3901 gctgacggtt gcctattatt tgaatctgga tgaaccctat tgggcgatga cctcggctgc
     3961 agtggttagc tttcccaccg ttggcggtgt catcagcaaa agcctcggac gcatggctgg
     4021 cagtttactg ggagccattg cggcactgct tcttgccgga catacgctca atgagccgtg
     4081 gttttttcta ttgagcatgt cggcgtggct tggcttttgt acctaggcct gtgcgcactt
     4141 cacgaataac gtcgcgtatg catttcaact ggcgggctac acggctgcca tcatcgcctt
     4201 tccgatggtt aatattactg aggccagcca gctgtgggat atcgctcagg cgcgcgtttg
     4261 cgaggtgata gtcggtattt tgtgcggcgg catgatgatg atgatcctgc ctagcagttc
     4321 cgatgctact gcccttttaa ctgcattgaa aaacatgcac gcccgattac tggaacatgc
     4381 cagtttactc tggcagcctg aaacaaacga tgccattcgt gcagcacatg agggggtgat
     4441 tgggcagata ctgaccatga atttgctgcg tatccaggct ttctggagcc actatcgttt
     4501 tcgccagcaa aacgcgcgcc ttaatgcgct actccaccag caattacgta tgaccagtgt
     4561 catctccagc ctgcgacgta tgttgctcaa ctggccctca ccgccaggtg ccacgcgaga
     4621 aattctcgaa caattgctga cggcgctcgc cagttcgcaa acagatgctt acaccgtcgc
     4681 acgtattatc gccccgctac gcccgaccaa cgtcgccgac taccggcacg tcgccttctg
     4741 gcagcgacta cgttattttt gccgccttta tctgcaaagt agtcaggaat tacatcgtct
     4801 gcaaagcggt gtagatgatc atgccagact cgcacggaca tccgctctgg ctcgtcatac
     4861 cgacaacgcc gaagctatgt ggagcgggct gcgtacattt tgtacgttga tgatgattgg
     4921 cgcatggagt attgcatcgc aatgggatgc cggtgccaat gcattaacgc tggcagcaat
     4981 tagctgcgta ctctactccg cagtcgcagc accgtttaag tcgttgtcac tgctgatgcg
     5041 cacgctggtg ttactttcgc tattcagctt tgtggtcaaa tttggcctga tggtccagat
     5101 tagcgatctg tggcaatttt tactgtttct ctttccactg ctggcgacaa tgcagcttct
     5161 taaattgcag atgcccaaat ttgccgcact gtgggggcaa ctgattgttt ttatgggttc
     5221 gtttatcgct gtcactaatc ccccggtgta tgattttgct gattttctta actataatct
     5281 ggcaaaaatc gttggcgttg cgctggcttg gctagcgttc gccattctgc gtccaggatc
     5341 ggatgctcgt aaaagccgcc gccatattcg cgcgctgcgc cgggattttg tcgatcagct
     5401 aagccgccat ccaacactga gtgaaagcga atttgaatcg ctcacttatc atcacgtcag
     5461 tcagttgagt aacagccagg atgcgctggc tcgccgttgg ttattacgct ggggtgtagt
     5521 gctgctgaac tgttctcatg ttgtctggca attgcgcgac tgggaatcgc gttccgatcc
     5581 gttatcgcga gtacgggata actgtatttc actgttgcgg ggagtgatga gtgagcgtgg
     5641 cgttcagcaa aaatcactgg cggccacact tgaagaatta cagcggattt gcgacagcct
     5701 tgcccgtcat catcaacctg ccgcccgtga gctggcggca attgtctggc ggctgtactg
     5761 ctcgctttcg caacttgagc aagcaccacc gcaaggtacg ctggcctcgt aattacttaa
     5821 ttacaccaca ggcatagcgt tcaccgccac cgcccagcgg tttaggttga tcggacatat
     5881 tatcgccgcc aacgtggacc atcagcgcct tgtctttgat ttcatccagt gatttcagac
     5941 gaggcgcgat gacggcatcg gtagctttgc cgtcattatt gacgaccagt gcaggcagat
     6001 cgcctaaatg cccggtacct tctggccctt catgtttacc ggtattttgt ggatcaagat
     6061 gcccgcctgc ggattccgcg gcgctggctt tgccatcttt ggtggctggc tggcagcttc
     6121 ctttggcatg aatatggaag ccatgttcac cggggggtaa tgctttcaga tcgggtgaaa
     6181 actccagacc tttatcggtt tcagtaatgg tgacgctacc aattgactgc ccaacccctt
     6241 gcgacgtgac gaggttcatc tcgacttttt cactggcagc ttgtgcgccg gtcgcaacaa
     6301 ccagcgccag aatggccaga ctaaaacgtt tcataggacc tccgttcatt gtgcattcct
     6361 gaataagtgt aaaccagtga cacatttttg aacggcggct attcctaaaa gtgcttacgg
     6421 tacgtcgtac cccagtgcag ctttacggat acgaaaccat tgttgacggg tcattttcag
     6481 tgtttctgct tcgacagctg cccgtacgcg ctcaatttta cctgagccga taattggcag
     6541 cggctgcgac ggtaaacgta atacccaggc gtaaaccacc tgttcaatcg agcccgcgtt
     6601 taactcctcc gccaccacag ccagttcatc acgcagcggc tggaaataat catcattaaa
     6661 cagacgacca ccaccaaggc aggaccacgc catcggacga acacgcagtt gttgtagttg
     6721 gtcgagcgtg ccatccagca gtaacggctg atgcaccggg gatatttcca cctgattagt
     6781 ggcaagggta aacggcagac gtgattgcaa cagggcaaat tgcgcaggcg taaagttcga
     6841 tacgccaaaa tgacgcactt tgccgctctg atgcagatgt ttgaacgcgt ccgccacttc
     6901 atcggcatcc attaacgggt ctggtcggtg gattaacagc agatccagat gatcggtcgc
     6961 gagattaatt agcgactgtt cggcgctctt aatgatgtga ttgcggtcag tgatgtaatg
     7021 accaatgacg ttttcttcac gcgcggtcgt cgcgataccg catttactga cgatttccat
     7081 ccgttcacgc aggtgaggtg ccagtttcag tgcctcgcca aacgccgctt cgcactgata
     7141 gccaccataa atatcagcat ggtccacggt ggtcacgccg agatccagat gctcttcaat
     7201 aaaactgacc agctggcggg cggacatatt ccagtccatc aatcgccagt agcccatcac
     7261 aaaacgggaa aactccgggc cttccggcgc aatagtaata cgctgaacca taatcgcttc
     7321 ctcttatcag atataagagg agtatacgca agattaggtt caaaagagtg atggttgctc
     7381 cggttcgtct gatgacgctg gcttatttgc gcgtaatttg cgcattaatc gctgccgaca
     7441 aaggcgcagc acctcttgtt tttcgccatc gctcatttta ttccagttaa aacgctcatc
     7501 ccgactacga aaacagccgc gacaaaaccc gcgttcgtca gactggcaaa ttccccggca
     7561 cgggctctgg acgggaaaga actctaattg ctccgccacc tcgccctcct cagataagat
     7621 tattaccatt attgaagctg ttaatgtcca aagtagcaac tttgcttgca ctagaccaac
     7681 tggtctacta cactccaacg catgaacaaa cacaccgaac atgatactcg cgaacatctc
     7741 ttggcgacgg gcgagcaact ttgcctgcaa cgtggattca ccgggatggg gctaagcgaa
     7801 ttactaaaaa ccgctgaagt gccgaaaggg tccttctatc actactttcg ctctaaagaa
     7861 gcgtttggcg ttgccatgct tgagcgtcat tacgccgcat atcaccagcg actggctgag
     7921 ttgctgcaat ccggcgaagg taactaccgc gaccgcatac tggcttatta ccagcaaaca
     7981 ctgaaccagt tttgccaaca tggaaccatc agtggttgcc tgacagtaaa actctctgcc
     8041 gaagtgtgcg atctgtcaga agatatgcgc agcgcgatgg ataaaggtgc tcgcggcgtg
     8101 atcgccctgc tctcgcaggc tctggaaaat ggccgtgaga accattgttt aaccttttgt
     8161 ggcgaaccgc tacaacaggc acaagtgctt tacgcactat ggcttggcgc gaatctgcag
     8221 gccaaaattt cgcgcagttt cgagccactg gaaaacgcgc tggcccatgt aaaaaacatt
     8281 attgcgacgc ctgccgttta gcaggcattt tttatcacca gacgaccggg agcctttatg
     8341 tcatctgaaa aactgtattc cccactgaaa gtgggcgcga tcacggcggc aaaccgtatt
     8401 tttatggcac cgctgacgcg tctgcgcagt attgaaccgg gtgacattcc taccccgttg
     8461 atggcggaat actatcgcca acgtgccagt gccggtttga ttattagtga agccacgcaa
     8521 atttctgccc aggcaaaagg atatgcaggt gcgcctggca tccatagtcc ggagcaaatt
     8581 gccgcatgga aaaaaatcac cgctggcgtt catgctgaaa atggtcatat ggccttgcag
     8641 ctgtggcaca ccggacgcat ttctcacgcc agcctgcaac ctggcggtca ggcaccggta
     8701 gcgccttcag cacttagcgc gggaacacgt acttctctgc gcgatgaaaa tggtcaggcg
     8761 atccgcgttg aaacatccat gccgcgtgcg cttgaactgg aagagattcc gggtatcgtc
     8821 aatgatttcc gtcaggccat tgctaacgcg cgtgaagccg cttttgatct ggtagagctc
     8881 cactctgctc acggttattt gctgcatcag ttcctttccc cttcttcaaa ccatcgtacc
     8941 gatcagtacg gcggcagcgt ggaaaatcgc gcacgtttgg tactggaagt ggtcgatgcc
     9001 gggattgaag aatggggtgc cgatcgcatt ggcattcgcg tttcgccaat cggtactttc
     9061 cagaacacag ataacggccc gaatgaagaa gccgatgcac tgtatctgat tgaacaactg
     9121 ggtaaacgcg gcattgctta tctgcatatg tcagaaccag attgggcggg gggtgaaccg
     9181 tatactgatg cgttccgcga aaaagtacgc gcccgtttcc acggtccgat tatcggcgca
     9241 ggtgcataca cagtagaaaa agctgaaacg ctgatcggca aagggttaat tgatgcggtg
     9301 gcatttggtc gtgactggat tgcgaacccg gatctggtcg cccgcttgca gctcaaagct
     9361 gagcttaacc cacagcgtgc cgaaagtttc tacggtggcg gcgcggaagg ctataccgat
     9421 tacccgacgt tgtaatccaa cattgcgagc ggcgtaaagc cgccgctata ctaaaacaac
     9481 attttgaatc tgttagccat tttgaggata aaaagatgcg tcttcttcat accatgctgc
     9541 gcgttggcga tttgcaacgc tccatcgatt tttataccaa agtgctgggc atgaaactgc
     9601 tgcgtaccag cgaaaacccg gaatacaaat actcactggc gtttgttggc tacggcccgg
     9661 aaaccgaaga agcggtgatt gaactgacct acaactgggg cgtggataaa tacgaactcg
     9721 gcactgctta tggtcacatc gcgcttagcg tagataacgc cgctgaagcg tgcgaaaaaa
     9781 tccgtcaaaa cgggggtaac gtgacccgtg aagcgggtcc ggtaaaaggc ggtactacgg
     9841 ttatcgcgtt tgtggaagat ccggacggtt acaaaattga gttaatcgaa gagaaagacg
     9901 ccggtcgcgg tctaggcaac taatctcctg ccgggcgtga actcatcgcg cccgcatctt
     9961 tactgcatcg acaagtaata tttgtcataa tgcgcgctgc aatttatccg tattaagaga
    10021 atcagatgtc cgataacgct caacttaccg gtctgtgcga ccgttttcgt ggtttttatc
    10081 ctgttgtgat cgatgttgaa acagccggat ttaacgccaa aaccgatgcg ctgcttgaga
    10141 ttgccgccat caccctgaaa atggatgaac aaggctggct gatgccggac accacattac
    10201 atttccacgt cgaaccattt gtcggcgcaa atttgcaacc agaagccctc gccttcaacg
    10261 gcattgaccc gaacgatccc gatcgcggcg cggtcagcga atacgaggcg ctgcacgaaa
    10321 tttttaaagt tgtacgtaaa ggtattaaag cgagcggctg taaccgcgcc attatggtgg
    10381 cgcacaacgc caattttgat cacagcttta tgatggccgc cgcagaacgc gcctcgctga
    10441 aacgtaaccc gttccaccct ttcgccactt ttgacactgc tgcactggcc gggctggcac
    10501 tcggacaaac cgtattgtca aaggcttgcc agaccgctgg catggacttc gacagcaccc
    10561 aggcgcactc cgcgctgtac gacaccgaac gcactgctgt gctgttttgt gaaatcgtca
    10621 accgctggaa acgtctggga ggctggccgc tacctgccgc cgaagaggtg taatcgagtc
    10681 gatgcctgat gacatgcaat gattcaggca tctatagtga ggctattcca cgcatcaagc
    10741 atgatattca cggggaatag cgttaatggc agataatcca gacccttcat cgctcctgcc
    10801 ggacgtgttt tcaccggcga cccgcgactg gtttcttcgc gcctttaaac agccgaccgc
    10861 tgtccagtcg caaacctggc atgtggcggc gcgaggcgaa catgcgctgg tgattgcacc
    10921 gactggctcc gggaaaacgc tggcagcatt tctctacgcc ctcgatcggc tcttccgcga
    10981 aggcggcgaa gatacccgcg aggcgcataa gcgtaaaacc tcacgcatcc tctatatttc
    11041 accgataaaa gccctgggca ccgacgttca gcgcaacttg cagatcccgt tgaagggtat
    11101 tgccgatgaa cggcggcggc gcggcgaaac ggtaattatt gatgaagtcc acgcggtagc
    11161 gggcagtaaa cgtggtgcgc atctggcgtt aagtctggag cggctcgatg cgctgctcca
    11221 cacctcagca cagcgaattg gcctttctgc cactgtgcgc tcagccagcg atgtggcagc
    11281 atttcttggt ggcgatcgcc cggttacggt agtcaacccg cccacaatgc gccatccgca
    11341 gatacgaatt gtcgtaccgg tcgccaatat ggatgatgtc tcatcggtcg ccagcggcac
    11401 cggcgaagac agccatgccg gccgggaagg ctccatctgg ccatatattg aaacgggtat
    11461 ccttgatgaa gtgttgcgcc atcgctcgac cattgtcttt actaattcac gcgggctggc
    11521 ggaaaaactg acggcacgat taaatgagct ttacgccgca cgcttacagc gttccccgtc
    11581 tatcgccgtt gatgcggccc atttcgagtc gacctccggc gcaacctcta accgtgtaca
    11641 aagtagcgac gtttttattg cccgctcaca ccacggctcc gtctctaaag aacaacgagc
    11701 aatcaccgaa caggcgctga aatcgggtga attacgctgc gtggtcgcaa cctccagtct
    11761 tgaactgggg attgatatgg gcgcggtgga tctggtgatt caggtggcaa cgccgctttc
    11821 tgttgccagt gggttacaac gcattggtcg cgccggacat caggttggcg gtgtatctaa
    11881 agggctgttt ttcccccgta cccggcgtga tttagtcgat tccgcagtca ttgtagagtg
    11941 tatgttcgca ggcaggctgg aaaacctgac accaccgcat aatcctctcg acgtccttgc
    12001 gcagcaaacc gttgccgccg cggcgatgga tgcattacag gtagacgaat ggtactcccg
    12061 cgtacgccgt accgcaccgt ggaaagatct gccaagacgt gtttttgacg ccacgctgga
    12121 tatgctttcc gggcgctatc cctctggcga tttttctgct tttcgcccca aactggtctg
    12181 gaacagggag accgggatat tgaccgcccg acctggcgct caattgttgg cggttaccag
    12241 cggcggcacc attccggatc gtggcatgta tagcgtgtta ttacccgaag gtgaagaaaa
    12301 ggccggttcg cggcgggtgg gtgaactgga tgaggagatg gtatatgagt cgcgggtgaa
    12361 cgacattatc actctcggcg ctacctcatg gcggatccag caaatcaccc gcgatcaggt
    12421 gattgtgact cctgctccgg gtcgttctgc ccagctcccc ttctggcgtg gtgaaggtaa
    12481 cggacgtccg gctgaattag gcgagatgat cggcgatttt cttcatttac tggcggatgg
    12541 cgcgttcttt tccgggacta ttcccccgtg gctggcagaa gaaaatacga acgccaatat
    12601 tcaggggttg attgacgagc aacgcaacgc gacgggcatc gttccgggga gtcgccatct
    12661 ggtcctcgaa cggtgccgtg atgaaattgg cgactggcgt attattttgc actctcccta
    12721 tggaagacgg gtgcatgaac cctgggcgct ggcgattgcc gggcgaatac atgccctatg
    12781 gggcgctgac gcgtctgtgg tcgccagtga tgacagcatt gttgcgcgca ttcctgacac
    12841 tgatggtaaa ttgcccgacg ccgcgatttt tttgttttaa ccagaaaagt tgctgcaaat
    12901 tgtccgcgag gcggtaggca gctcggcact tttcgccgcc cgttttcgcg aatgcgccgc
    12961 gcgggcatta ttaatgccgg gccgcacgcc gggccatcgc accccgcttt ggcaacaacg
    13021 gctgcgcgcc agtcagttgc tggaaatcgc tcagggatat ccggattttc cggtcattct
    13081 cgaaacccta cgcgaatgtc tgcaagatgt ttatgatctt cccgcactgg aacgtttgat
    13141 gcgtcgcctg aacggtggcg aaattcaaat atccgatgta acgaccacta cgccctcgcc
    13201 tttcgccaca agtttattgt tcggctatgt cgcggaattt atgtaccaga gcgacgcccc
    13261 gctggcagag cgccgggcat ccgtactgtc gctggacagc gagttactgc gcaatctact
    13321 cggacaggtc gatccggggg aattactcga cccgcaggtc attcgccagg tggaagaaga
    13381 gttgcaacga ctggctcctg gtagaagagc gaaaggtgaa gaaggattgt tcgacctgct
    13441 gcgcgaactg gggccaatga ccgttgaaga cctggcgcaa cggcatacag gcagcagtga
    13501 agaggttgcg tcgtatctgg aaaatcttct tgcagtaaaa cgaatcttcc cagcgatgat
    13561 tagcggacag gagcgtcttg cctgtatgga tgatgccgcc aggctgcgtg atgccctcgg
    13621 cgtacggcta ccagagtcat tgccagagat ttatttacat agagtcagtt acccgcttcg
    13681 cgacctcttt ctgcgctatc tccgggctca tgctctggtc acggctgaac aactggctca
    13741 tgagtttagt ctcggtattg ccattgtcga agagcagctt cagcaactgc gtgaacaggg
    13801 tctggtgatg aatctgcaac aagacatctg ggtgagcgat gaagtatttc gtcgtctgcg
    13861 tttgcgctcg ctgcaagccg ccagagaagc gacgcgtccc gttgcagcca cgacctatgc
    13921 gcgattgctg ctggaacgtc agggcgtatt acccgccacc gatggtagcc cggcgctctt
    13981 tgcctcaaca tctccaggcg tttatgaggg cgtagatgga gtgatgcggg tgatcgaaca
    14041 gcttgccgga gtcggtttac ccgcctcact ctgggaaagc cagatcctgc ctgcccgcgt
    14101 acgcgactat tcgccagaaa tgctcgatga attactggca accggagcgg ttatctggtc
    14161 gggacaaaaa aaagctgggt gaagatgacg gcctggtggc actgcatcta cagaaatatg
    14221 ccgcagaatc gttcactccc gccgaagcgg atcaggcgaa tcgttcggcg ctgcagcaag
    14281 cgataatcgc tgttctggct gacggaggag cctggtttgc acaacaaatc agccaacgga
    14341 tacgcgataa aatcgtcgaa tcggttgatt cctcggcact gcaagaggcg ttatgggcgc
    14401 tggtctggca aggcgtcatt accagcgata tttgggcacc gttacgcgcc ctcacccgca
    14461 gcagttccaa cgcacgcacc tcaactcgcc gcagtcaccg ggctcgtcgt gggcgtcctg
    14521 tctatgcaca acccgtctcg ccgcgggtat cttacaacac accaaatctg gctggacgct
    14581 ggtcgttatt gcaggtggag ccactaaacg ataccgaaag gatgctggcg ctggcggaaa
    14641 atatgctcga ccgctacggc atcatcagtc gtcaggcggt gatagccgaa aatatccctg
    14701 gcgggtttcc atcgatgcaa acactttgtc gaagtatgga agactccggg cgaattatgc
    14761 gaggtcgttt tgtagaaggt ctgggtggcg cgcaattcgc tgaacgtctg actattgacc
    14821 gattgcgcga tctggcgaca caagccacgc aaacacgcca ctatacacca gtggcgctct
    14881 ccgccaacga tccggctaat gtgtggggaa atcttctgcc ctgggctgca catccggcaa
    14941 cgctggtacc aacgcgtcgg gcgggtgcgc tggtggtcgt ttctggcggc aaattgttac
    15001 tctatctggc gcaaggcggc aaaaaaatgc tggtctggca ggaaaaggag gaattactcg
    15061 ccccagagat tttccaggcg ctgaccaccg cactgcgtcg cgaaccaagg ctgcgcttta
    15121 cgctaacaga agtgaatgat cttccggtcc ggcaaacgcc gatgtttacg ctgctgcgtg
    15181 aggcgggatt ttcaagttcg ccacaagggc tggattgggg atagttgaca tcctccacgc
    15241 cctgaaagac gtggttccct gctacgttca agctgtcgcc tgaatcattt cggtgggttc
    15301 ctgcttcaac gagcggcctg actgcaccat ctctccacag gcaagaacgg cgtgccccgc
    15361 cgctaaaatg ttacgagcgc catttacatc ggcgttcgct gtatatccac atacctggca
    15421 tctgaattta ctttgtgaca ggcggttttc tttcgctgta tgaccacagc acgcgcaacg
    15481 ctggcttgtg tacgctggcg gaacagcaag cacctgaccg ccactccaga gctgcttgta
    15541 ctcaagctgg cggcgcattt cataccagcc ctgatccagt atcgaacggt ttaaacctga
    15601 ttttgcccgg acattgcgcc ccggctggct gaccgtaccc gctgctgact ttgacatgtt
    15661 gctgaccttc aaatcctcaa tgactatcat tgcgtggttt ttgctgacgg tcgttgtgac
    15721 tttgtgaagg tagtccctgc ggatatttgc gatacaggaa tgcagtcgct gtattttgcg
    15781 tttctgcttc tgccagttgt tgctgaattt gaccttccgg cttaactgtc gctgaagtct
    15841 cgccagcgtc ttctggtttt tttgaaaact gtttacaggc tcaaagactg tgccatctga
    15901 cagcgtggcg agtttagcca cgccagcatc cagcccgatc attgatgctg aagggtgaac
    15961 cggagttgat acttcacttt ctgtctgaat actgatgtac cacgtaccgc aggactggct
    16021 gacagtgaca tttttcacaa ctcccgtgac ctgacggcta ttccggtagc gcatccagcc
    16081 aagtttcggc agaaaaatac ggctgttttc ctgatcgagc ttaacgtcct gcgggtagcg
    16141 gaatgcagca ttctgtcccc gctttttgaa tcggggaaaa gcagcccgct tctggaagaa
    16201 gtttttgtaa gcccgctcaa ggtctttcag tgactgttgc aatggctgtg agggcgaatc
    16261 tttaagccat tgcgtttcag tggcgttttt ccactcaacc agccaggaag ccattttccc
    16321 gtaagggatg tatttattac cggcctcatg attctcattc tgaagcgcca gagcacgatt
    16381 gaaaacgaaa cgacatgcgc ctgcgaagcg cctcatttca cgctcctgtt gaccaccggg
    16441 tcttaactgg aatttaaatg cctgtaatcg tttcacaggt caaatgatac tttggtctgt
    16501 gaaaaaagaa accgatatcc ggcgtggaag gcattgtgtt ttcctgatgc atgttcacct
    16561 ggtctttgtc accaaatacc gacgccagat ttttgaccat gacacgacag aaaaactacg
    16621 cacttacttt tcaaatgtat gtgctgattt tgcagctgaa ctggttgaaa tggatggcga
    16681 accagatcac gtccatttgt taattaacta tcctcccaaa ctggcgatat ccagtctggt
    16741 aaacagcctc aaaggcatat cgaataggtt actgcgacga gatcgaccag atattgcagt
    16801 caggtattac tacaaaggcg ttttgtggag tcctggctat tttgccagta gctgcgaagg
    16861 tgcgccaata tccgtcatcc gccagtacat tgaacaacag caaacacctg gtcaggtgga
    16921 aaaccgcgcc ttatatcccc gccctgaagg acgaggtttt acggcgcacc ggataaagga
    16981 ctgacggatg cccgttcgca tccgtcagta ttgcaggacg gattattccg cgtccggctc
    17041 ttcagacttg tatttagcgg cagtttcttt gatcagctgc tgcagttcgc cacgctgata
    17101 catttcgatc acgatatcac aaccgccgac cagctcgccg tcaacccaca gttgcgggaa
    17161 ggtcggccag ttagcatatt tcggcagttc cgcacgaatg tccggattct gcagaatatc
    17221 aacataggca aaacgttcgc cacatgcggc aagcgcctgg actgcctggg cagagaaacc
    17281 gcagctcggc agtttcggtg aacctttcat gtacagcagg atcgggtttt cagcaatctg
    17341 gcgttggatt ttttcgatag tggtgctcat tgtcttgctt cctcaaactt ctttacggca
    17401 gtaatctgac attgtagcgg gtcagtgcgg catcggaaaa taacattttc atcacgcttt
    17461 tgctatttta tccctttgct ctatttgttg catttcaaat attcgtttct tctctgatgc
    17521 gcggcaaact ggcgtattta taaccatttt tagctttcac tgctgcattt ttttgcgctc
    17581 gccaacgaaa cgtatttttt aacaataaaa gctattaact ttctcttctt ctatgcatta
    17641 gaatcatcaa gttttgtaaa tcagacgcag gcatgataga cctgccttaa cagagggacg
    17701 ctcagtggcg cggataaacc gtatttcgat cacgctctgt gctttacttt ttaccaccct
    17761 gcctttaacg cctatggccc atgcttcaaa gcaagccagg gagagttctg ctaccactca
    17821 tatcaccaaa aaagcagata aaaagaaaag cacggcaacc accaaaaaga cccagaaagc
    17881 cagccagaaa acagcgaaaa aagccgccag taaaagtatg accaaaagca aaaccgcttc
    17941 ttctgttaaa aaatcttcca ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc
    18001 cgtcaataaa acggcctcag ccagctttac cgaaaagtgt accaaacgta agggctataa
    18061 atcgcattgt gtgaaagtca aaaatgccgc gtcaggaacc cttgccgacg cgcacaaagc
    18121 gaaggtgcaa aaagccacga aagtggcaat gaataaactg atgcagcaaa ttggtaagcc
    18181 atatcgttgg ggcggcagct caccgcgtac cggttttgat tgcagcggcc tggtttatta
    18241 cgcttataaa gatttggtga aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct
    18301 gcgtgatgca ggacctatcg aacgtagtga gctgaaaaac ggcgacctgg tctttttccg
    18361 tactcaggga cgcggtacag ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat
    18421 tcagtcaccg cgcacaggtc aggaaattca aatcacttct ctcagtgaag actactggca
    18481 gcgccactat gttggcgctc gtcgggtaat gaccccaaaa acacttcgct aaaactttac
    18541 cctgttgtta cggcaacagg gtaagttcat cttttgtctc accttttaat ttgctaccct
    18601 atccatacgc acaataaggc tattgtacgt atgcaaatta ataataaagg agagtagcaa
    18661 tgtcattcga attacctgca ctaccatatg ctaaagatgc tctggcaccg cacatttctg
    18721 cggaaactat cgagtatcac tacggcaagc atcatcagac ttatgtcact aacctgaaca
    18781 acctgattaa aggtaccgcg tttgaaggta aatcactgga agagattatt cgcagctctg
    18841 aaggtggcgt attcaacaac gcagctcagg tctggaacca tactttctac tggaactgcc
    18901 tggcaccgaa cgccggtggc gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt
    18961 ttggcagctt tgccgatttc aaagcgcagt ttactgatgc agcgatcaaa aactttggtt
    19021 ctggctggac ctggctggtg aaaaacagcg atggcaaact ggctatcgtt tcaacctcta
    19081 acgcgggtac tccgctgacc accgatgcga ctccgctgct gaccgttgat gtctgggaac
    19141 acgcttatta catcgactat cgcaatgcac gtcctggcta tctggagcac ttctgggcgc
    19201 tggtgaactg ggaattcgta gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag
    19261 cattgcctga tgcgcttcgc ttaccatgcc aacgcggatg aggcgttaac agcgcattcg
    19321 gcaagataaa tcgcacgttg tcagcaactg taacgcagaa ggttatcctt ctgcgttttt
    19381 gtttaattag ctgttagcaa cgcaaactgt ttcaggttgt tttctggctg acataaacac
    19441 cagcaataat gccagtcccg cgacaatcgc ccccgtcacc ggcacaaagc tgtatcccag
    19501 cccagcggaa attaccgcac caccagcagc tgctcccagc gcatttccaa gattaaaggc
    19561 accaatattg actgatgaag acagacctgg cgcttcactg gcgacatgca tcacgcgcat
    19621 ctgtaacgga ggtacaaccg caaaggttgc tgcgccccac accaccatgc taatagctgc
    19681 gccgaactca ttgcgggcca ggaacgggat tgccagcata atcaccatca gcagcaacaa
    19741 aaagcctttc aacgtgccgt taactgaacg atctgccagt ttgccgccga gatagttacc
    19801 gatagagaat ccgacaccaa tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg
    19861 ggtaatactt tgcagtaccg gagagatata ggtgtagagg gtaaacattg caccagctcc
    19921 cagtaccgtc gtcagcaatg cagacagcac ctgcggacgc attaataccg ccagctcttt
    19981 tttcacttca ggtcgtgccc ctgtaccacc tttaggtaat gagaagaaca gacttaccat
    20041 tgaaatcact cccagccccg ccgttgccag aaatgacatc cgccagccga tggtttcacc
    20101 caaccaggtc gccgccggca cgccaccgat atttgccagg gttaacccca taaacatagt
    20161 ggcaactgcg ctggcctgtt tatgttttgg caccacgctt gcggccacga ctgaacccaa
    20221 accaaaaaat gctccgtgat tcaggctggt caaaatacgt gaaagcatca gggtcatata
    20281 atccggcgcg atggcggaaa gaacgttgcc tagcgtgaaa attgccatca ggaaaatcaa
    20341 cgcactgcgg cgggcacgat gagaaagtag aagcgtcatc agcggcgcgc ccaccattac
    20401 gccaactgca taggcactga ttaacattcc ggcagcggga atcgagacat ccacaccgcg
    20461 cgcaatgacg ggcaacaagc ccattggcga gaactccgtt gtcccgatac caaacgcgcc
    20521 aatcgccagc gccagcaacg gatagttaat tttcatgcct tatctccacc tcttcgcgtc
    20581 attacgcgat attcattaaa gtggcgaaag catgacagca atcacaaaaa aatgaaaata
    20641 acaaaaagag aaaacacttt tgccattttg ctaacaaaca ggaaggagat gcgagggaga
    20701 acgcgctccc tcgagaggaa atcagtgcag cgcggcagtc aaacccacgg ctacgatcaa
    20761 accgaggacg ataaccgttg ttaccagtga aaatttaagg tcggtgctca tcaagttttc
    20821 tcctttttta ttaccacaca aaaagtgata ttacgcattt tt
//
LOCUS       DAETQL010000069        20780 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.069, whole genome shotgun sequence.
ACCESSION   DAETQL010000069 DAETQL010000000
VERSION     DAETQL010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20780
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.069"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..579
                     /locus_tag="K0W65_002812"
     CDS             <1..579
                     /locus_tag="K0W65_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI3096011.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="K0W65_002813"
     CDS             597..1235
                     /locus_tag="K0W65_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI3096012.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="K0W65_002814"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="K0W65_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBI3096013.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="K0W65_002815"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="K0W65_002815"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBI3096014.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="K0W65_002816"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="K0W65_002816"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBI3096015.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="K0W65_002817"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="K0W65_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBI3096016.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="K0W65_002818"
     CDS             complement(5552..6613)
                     /locus_tag="K0W65_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBI3096017.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="K0W65_002819"
     CDS             complement(6610..8007)
                     /locus_tag="K0W65_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBI3096018.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="K0W65_002820"
     CDS             8163..9161
                     /locus_tag="K0W65_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI3096019.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="K0W65_002821"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="K0W65_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="K0W65_002822"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="K0W65_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="K0W65_002823"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="K0W65_002823"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBI3096020.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="K0W65_002824"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="K0W65_002824"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HBI3096021.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..14855)
                     /locus_tag="K0W65_002825"
     CDS             complement(14232..14855)
                     /locus_tag="K0W65_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBI3096022.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14852..15274)
                     /locus_tag="K0W65_002826"
     CDS             complement(14852..15274)
                     /locus_tag="K0W65_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBI3096023.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15296..16071)
                     /locus_tag="K0W65_002827"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="K0W65_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16089..17141)
                     /locus_tag="K0W65_002828"
     CDS             complement(16089..17141)
                     /locus_tag="K0W65_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBI3096024.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="K0W65_002829"
     CDS             complement(17172..18014)
                     /locus_tag="K0W65_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBI3096025.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="K0W65_002830"
     CDS             complement(18001..18882)
                     /locus_tag="K0W65_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBI3096026.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="K0W65_002831"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="K0W65_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20200..>20780)
                     /gene="ycjM"
                     /locus_tag="K0W65_002832"
     CDS             complement(20200..>20780)
                     /gene="ycjM"
                     /locus_tag="K0W65_002832"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HBI3096027.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5071 a         5668 c         5053 g         4988 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagtttacta
//
LOCUS       DAETQL010000070        20712 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.070, whole genome shotgun sequence.
ACCESSION   DAETQL010000070 DAETQL010000000
VERSION     DAETQL010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20712
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.070"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(76..4536)
                     /gene="mukB"
                     /locus_tag="K0W65_002833"
     CDS             complement(76..4536)
                     /gene="mukB"
                     /locus_tag="K0W65_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBI3096028.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="K0W65_002834"
     CDS             complement(4536..5240)
                     /gene="mukE"
                     /locus_tag="K0W65_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBI3096029.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="K0W65_002835"
     CDS             complement(5221..6543)
                     /gene="mukF"
                     /locus_tag="K0W65_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBI3096030.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="K0W65_002836"
     CDS             complement(6540..7325)
                     /gene="cmoM"
                     /locus_tag="K0W65_002836"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBI3096031.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7461..8240
                     /gene="elyC"
                     /locus_tag="K0W65_002837"
     CDS             7461..8240
                     /gene="elyC"
                     /locus_tag="K0W65_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBI3096032.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8217..9110)
                     /locus_tag="K0W65_002838"
     CDS             complement(8217..9110)
                     /locus_tag="K0W65_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBI3096033.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="K0W65_002839"
     CDS             complement(9264..10010)
                     /gene="kdsB"
                     /locus_tag="K0W65_002839"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBI3096034.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="K0W65_002840"
     CDS             complement(10007..10189)
                     /gene="ycaR"
                     /locus_tag="K0W65_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBI3096035.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10241..11473)
                     /locus_tag="K0W65_002841"
     CDS             complement(10241..11473)
                     /locus_tag="K0W65_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HBI3096036.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="K0W65_002842"
     CDS             complement(11510..12496)
                     /gene="lpxK"
                     /locus_tag="K0W65_002842"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBI3096037.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="K0W65_002843"
     CDS             complement(12493..14241)
                     /gene="msbA"
                     /locus_tag="K0W65_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBI3096038.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14278..16537)
                     /locus_tag="K0W65_002844"
                     /pseudo
     CDS             complement(14278..16537)
                     /locus_tag="K0W65_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="K0W65_002845"
     CDS             complement(16744..17028)
                     /gene="ihfB"
                     /locus_tag="K0W65_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBI3096039.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="K0W65_002846"
     CDS             complement(17188..18861)
                     /gene="rpsA"
                     /locus_tag="K0W65_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBI3096040.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="K0W65_002847"
     CDS             complement(18972..19655)
                     /gene="cmk"
                     /locus_tag="K0W65_002847"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBI3096041.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="K0W65_002848"
     CDS             complement(19828..20592)
                     /gene="ycaL"
                     /locus_tag="K0W65_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HBI3096042.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4980 a         5727 c         5032 g         4973 t
ORIGIN      
        1 cgttatgtgc cattttttta gtgcagaaac ggagttttcg gaaaaagaaa aggcggcatt
       61 gctgccgcct taattttaac tcgcctggga aggcgcttcg tctcttccta gaagcgtttc
      121 agggagttgc ggcgcaaatc ctcgcaggcc gacgacgtga acatgttcgg tattctggaa
      181 gactttacgt accagtttat aggtggtgcc tttttccgga ctgatatttt ccggcgctgc
      241 gatgatgagc tgcatttgca aacgttcaca caattcaaac agcgtggcga tagaacgagc
      301 atccagtcgc gctgcttcat cgaggaacag caggcggcaa ggagagatat ctttaccgcg
      361 caggcggcga gattcatctt cccagctttg taccaccatc accagaatcg acataccggt
      421 accaatcgcc tcaccggtcg acaatgcacc agactctgcg cgcaaccaac catcggaacc
      481 acggttaacc tcaacttcca tttccagata gttgcggtaa tccagcagtt cttcgccaat
      541 ggtctgcggc gtgcgctgcc ccatatcaat ctgcggatta agacgctgat acaatttcgc
      601 caacgcttcc gagaaggtca gacggttgct gttaaacaga tcctgatgct gctcgtgctg
      661 ttcagagagc acatccagca gcatggcgtg cgtttcacgc acgttcacgt tgagacgcac
      721 gctgttcacc tgaccaaacg atacgttctg caacccctgg ttgagcatac ggatacggtt
      781 ctgctcgcgc tgaatggttt tgcgaatgat gttcgccacg ctgcgggaac tgatcgccag
      841 tttctgttca cgggaggtta attcttcggt cagacggcta agttcaatct ccatctgttc
      901 gatagcttcc accggatcat cggtacgaat aatatcctga cgaatacgtt cacgcagatg
      961 ctgataaacc gccacgaaga actgaatttt acgctccgga cgtttcggat cttccgacat
     1021 gcgcagcacg tcgcgcagat gttcgttatc cgccaccgcc agacgcagcg cacctaacgc
     1081 cttatccgac atggaacgca aatcatcagc ggagagataa gccagctcac gacggtgtaa
     1141 gcggcgctca acgccgttat ctttcaccat gcgcatcacc gcacaccagc ccgctttggc
     1201 ggtcactacc tgctcgcgca tctcaaagta atcccgctcc agcttgcgca gtttgcgggt
     1261 caggttgtcc atctccgctt cgcagaaggt aagcgctttt tccagttgat tgcggcgtga
     1321 acggttattg ctcagttgcg catgcagctc gtcacggcga atacgcgccc gctcttctgc
     1381 cccgctatca gcacgcacgc cgatatcctg caattcacgt tgcagatcgt tgagtagctc
     1441 ttttttggtg tcgtaagaac ttttcagcga agccagcacc tggttgtact gactcaactg
     1501 cgctgcgtga ccgcgcaacg cttcgcgagc gcgggtacgc tccgcttccg cctgttccag
     1561 acgttcacgc agtttttcgt tgagatcgct gttaccgcta agcatttctg ccgagtcaga
     1621 atagctaaag tgcgcacgac gctgcaccac ttccgtcagg gcaaacgctt gctgacgggc
     1681 atcgcgctgc atctgctgag agtacgcgta atcttctttt aactgttcga actgttccgg
     1741 gtcgctctgc aataccgaaa cgatcggttc cagtttcgcc agttgattgc caaactgctg
     1801 aacaaaacgc gcggcttcct gggcttcatc cagacgttcg cggatttcat cgacgcgatc
     1861 cgccaggctg tcatcagcca acaggttgag acgcggcaga atgcggttca gcgccgtaac
     1921 gccttctttc gcctgctcaa actgaatacg ctgctgctgg ttatcatttt catgattact
     1981 caacgcccgc tccagttcga ctcgacggct gttcagttga cggatttctg cttccgggtc
     2041 agactcaaac gccaccgcca gatgactgcc gataaagcgg ctgaacgcct gatgcagacg
     2101 ctgggttttc tgtacatcaa aggagagcgt ggcgaagcgt tcggaaagca cttcacgctc
     2161 ggcatggagg ctttcaatac ggctttcacg cgcagcacga ccaaacagcg gcacttccgg
     2221 gaaacgtgaa taacgccact gtcgatcggc gattttcacc accaccgctt tttccagctc
     2281 atcaacgctg aacacgctat catcgaatga ctgcggatcc ccttcgatca aatagagatc
     2341 ttccgggcaa tcggtcaagc cttccaggtg ttcggttacc tgtgacagat ctggcaccac
     2401 gatggcgtgg cgtgacgggc catacagagc cgagaagtac ggcgcatctt ccaggctaac
     2461 gtcgtcataa atttctgaca gcagcacacc accaaaacgc tccgccagcg cgttcagacg
     2521 ctgatcttca gagccgccag gctggcttaa acgttcgatc tcttcatcga cggcgttttt
     2581 gcgcgcgcct acttcatcgc gttcaacaat cgcctcgcgc tcacgctcca gcaactgttg
     2641 cagatattca gtgacgtcct ggctggagga gaactcttcg ccgcactgtt cactcaactg
     2701 gttgagactg ttttgcgctg ccagccaaac cggtgcacgc tgcatcaaac tctgaatgcg
     2761 agactgcagc tgttcctgtt cctgccgcag tgccatccgc tcttcacggg cgttagatac
     2821 gctatcggaa agagaggcaa tgcgtgcttc cagctcctga tgcagagctt ccagttcatc
     2881 aatatcaaaa ttcttgccct gacgtttgca gaaatctgcc agcagacgct cagcttcttg
     2941 ctgctcacgc agacgctgtt ccagttcgct taagcgcatc cgcaacggct gaacctgctc
     3001 tgccaggtga cgctgatcaa ccccttcgcg caatagttcg cgagcgacat cccacgcctc
     3061 gttacgcgcc agcgggccgt tgattgccac caccagctga taagcctgct caaactggct
     3121 gtgcgcggtt tgcgccatgc tcattttctg ctcaagagag agcatttttt cagtcgcttc
     3181 cagctctttc gcctggaagg tttccagcca ttcggcggcg ctgtcggcgg ttaagtccgg
     3241 cagatggcac agttctttgg cacgattaag cgcagcaatc gcctggttat actggatcgc
     3301 gcgcgtttgc tggacgtcca gcgcctgctg gtagtcagca agctggcttt tcagctcgtc
     3361 cacttccagt tcggcagctt ccgcgcgagc ctcattctct tcctggcgtt cgatggcttc
     3421 tgccaccact tcattttgct cttccagacg gatctgcagc tcatcgagat ccacttcgta
     3481 gcgttcgatc ttctcctgct gacgcaatgc ggtttgcacc aggttcaggt gatcgctggc
     3541 cgcctgataa tccgcttcca gatcaccttc ggcaccgttg tgctctgcca gctcacgcgc
     3601 catatcgacg tgtttgtact gctcagccgc cagttgctga cgcgaagtat gtagctcgcg
     3661 acgaaactcc agggctttgt cgagatggac acggcgctcg ttggcgtgac gcatgtagtc
     3721 cgccgccacg tagttggtag cttcgctgat cagatgctta aacaggtcac ggtccgactg
     3781 ggtgacacga atcgcttcca gcgtcatgcg gttttcacgc agcgccgctt ccatgtcctg
     3841 gaacgcttta cgcacgccgc tgttttccgg caacaggtag tcgcgcagag aacgggtaat
     3901 ggcactggag atcccgccat acagcgaagc ttcgatcaga cgatagaatt tgctacggtc
     3961 agatgcggag cgcagacgac gcgcgatgat gcccagatcg aacatcagcg agtggtaatc
     4021 ggtaatggag ttgaactgtt taaactgcac gccttccatc gcctcgagct tgtctttcag
     4081 ctcgttaagc ggcagcacgc gcgcctggcg ttcgttcagg gtttcggtca ccagctgtgt
     4141 cggctgcacc gacatcggca gtccctgaat ggcaaatggt ttgatatcca ctttacgatc
     4201 gcgtccggca acctgttgca gacgcacacc gaccaccacg cgctggtggc gcgagttaat
     4261 ggtgtcgagc atcgaataac agacacccgc tttcagctta ccgtgcagac ctttatcgcg
     4321 cgaaccgctg gtggccccgg cttccgtagt gttacggaaa tgcagcaggg tcaggtcggg
     4381 gatcagcgcc gtaacgaacg ccgccatcgt ggtggattta cccgccccgt taccgccgga
     4441 aagcgtcgtg accagctcgt caaggtcaaa agttcgggca aaaaagccgt tccagttaat
     4501 cagcgtcagt gagcgaaatt taccgcgttc aatcattatt cttcctctcc gctatctggc
     4561 tgactctctt cggtttcatc gttgagttgc agatgatttt caatcggcat tgcttcgcca
     4621 tcacgaatca ggcgacgctg tgcttcacgg ggatcgtcgc cagcacgcac atcggctccg
     4681 aagcggaaca ccgattcggt aatgcggaac ttgctgctgt cgtggcccat aaaccacacc
     4741 atgcctaaac gacgcagacg gttgagcgaa gaacgtactt tctcctgcaa cttctgacgg
     4801 tcaacgtctg aaccggttga acggttgttc accagtttca gcagtttcgc ttcatcggcc
     4861 agggtgagca gttcgtcgta cagttcctgc tgggtgaaaa tcccctcatt cgccagccgt
     4921 tccgggctga gatagagata acagaggatt ttcccgacca tcatatccag ttccgacaag
     4981 acggaacgcg ggataagagt ggtggaacgt gggcgtaaat agaagaaccc ttccggtgcg
     5041 cgaataagct caacgttata acgcgcgtaa aactcttcca gatattcctg aaaatccatc
     5101 aggaatgcat gattatccag ttcgtcgagg ccaatatggc ggcctgaacg taaggcgctg
     5161 tccagcgccg gaaataacgg attcgccagc gcctgcgcca gcttaaccgg catcacttgt
     5221 tcaatatttg tcgatgacat gcgcctgtac cttggctccg taatcattaa tcggctgcca
     5281 tttcgctggc agtccggtga aatctgcttg cgctacgcca agacgtaccg cctgatcaat
     5341 aacaatacgc gcaacgtcaa agtgacgtgc acgcggatac tgtgacagat attcgcgtac
     5401 caccagacca agatccagcg gcacttgtct ggttttgtac acggcaagtt gttcttcgat
     5461 gatcgccgcc agctgttcgc ggatctcgtt aaactcttcg tattccagat cctcaggaag
     5521 ttccccagtc acttcttcat cgcgcagtgc catctcttcg tcacgcatat ccagcagacg
     5581 atcggcattg gcataagtta gcgcccacgg ctcatcaaaa taggtttgta ccgactgacg
     5641 taaccgctga gcaaagacgc ggtttttatc catatcgatc gcggtacgaa taaatttgtg
     5701 tacgtggcgg tcgtagccaa tccacaagtc gatggattgc tggccccaac tgataatacg
     5761 atcgagtttg ctctgcagat cgaacaccag acgatcgacg aaatgcagat cgtcatgggt
     5821 catcgtcgca tcctgaatgc gcaacagatt agcctgcaat ttgtcgcctg ccgcttccag
     5881 cgtatcctgc aattcacgca gcgttccgga agtttccgaa agcaacaatt cacagctgga
     5941 aatcgccgcc cgccagtctt tgttcagcaa ctgggcgata tcgtccttca cctgctgctg
     6001 ctgttcgtcc atcagacgtt gcgtcaggtc gatactgtcg aaaatttctg ctaccgaata
     6061 tttcagtggc gcatagacat tacggtgcca gtgaaattca tcaccgccct cttcggcggc
     6121 atctgctgcg cgtttgagct cacccgccac aatcgacaac tgcatagaaa gacgcagcgt
     6181 agaaaactcg cgctgacgga tgtagtagtc agtaatgccg atgccgagcg gcgtcagacg
     6241 gtaaattgcg ttcccttccg cctgctcgct ggtaaagcgg ttcagcagac gttgacgcac
     6301 catgtcgttg atcgcgttat tggcgcgcac gccgatggtt tcgctggttt gctcaaacgc
     6361 atcactcaca tggcggaatg catccaccag ctcgccttca ctcatctcac catccagacg
     6421 ctcgccgttc agcgtggcaa ccgccagcag aaaagagagt cggtctaccg gcagcgagat
     6481 ggagaagtca ttttttctgg cccaggcaac cagttcgggg actgtctggg aaaattcact
     6541 catactttat ccttgctctg cggtttgcgc gcggtgacat gaatataacg ccctagggta
     6601 atatacggtt cctgacggca ataacgcgtt tctaattcaa gtaatgcttc atagcagtcg
     6661 cgctgctggt acttctcgcg cagataatca tgaaacacgc gaacgcctgt cttacccata
     6721 atttgccaac cagcttcttc cagccacaga taaacctgcg ccgggtcgcg tggataatct
     6781 ggcgaaagcg tccgtttttt ctttttcggc attcccgcct gcacgtaatc aaaattcccg
     6841 gcgaccatgt tatgcatcaa caaaccatgc gcattgtaga acattaacga caacactccg
     6901 cctggacgca acactgacca cagggtctgc aatacactgc ggggatcggc cacccactcg
     6961 agcaccgcat ggaacaatat cagatcaacg ggcgtttcca aatgcgaagc aacatcctga
     7021 gcggcgcaat gtataaattg catgttgtcg ctcacacctt ttgcttctgc cgcctgtttt
     7081 gcgcggtcga tcatctgcgc agaaagatcg cataaaataa cctgatgccc gcgctcggcc
     7141 attttgattg cggtctgccc ttctccgccg ccagcatcca gcacacgcag tttttgcggg
     7201 cccatttccg ccagcacgcg atcgagatcc tgccacagaa tagcctgtcg aagctgccct
     7261 ttggtggtgc cgtaaatgtt acgggaaaac ttttccgcaa tatcatcaaa attgcgatcc
     7321 tgcattgctt tctccaccgg ttggctaaga cgttttctga taaagacgct attttgtcac
     7381 agccacggcg agaatgaacc cgtctggtgt aatatcgcta gcgatccctt gctatatggt
     7441 taaaaaagga accagaaagg atgcttttta cactgaaaaa agtgattggc aatatgttgc
     7501 tgccacttcc gctgatgttg ttaattattg gcgctggcct ggcgctgctg tggtttagcc
     7561 gttttcaaaa aaccgggaag atttttatta gcattgggtg gctggcactc ttgttattga
     7621 gtttgcaacc ggtagccgac cgcctgctgc gtcccatcga aagcacctat ccgacatgga
     7681 ataattcgca aaaagtggac tatatcgtgg tgctcggagg tggttatacc tggaacccgc
     7741 agtgggcacc gagctctaat ttaatcaata acagtctgcc acgcctgaat gagggtatcc
     7801 gtttatggcg cgaaaatccg ggttcaaaac tgatcttcac gggaggcgta gcaaaaacca
     7861 atacggtgag tacagcggaa gtaggtgcca gagttgcgca atcgctgggc gtaccgcgcg
     7921 agcaaattat caccctggat ttgccaaaag ataccgaaga agaagctgcg gcagtgaaac
     7981 aggcgattgg tgacgcccct ttcctgctgg tgacctccgc atcacatctg ccgcgggcga
     8041 tgatcttttt tcagcaggaa gggttaaatc cgctaccagc cccggcgaat cagttggcca
     8101 tcgactcgcc gcttaatccg tgggaacggg caatcccttc tccggtctgg ctgatgcata
     8161 gcgatcgtgt tggctatgaa acgcttggcc gcatctggca gtggctgaaa ggtccgtcag
     8221 gcgagccacg gcaagagtga ttttgatgcc agatcgaagt tgcgccgact aaatcgtccg
     8281 gtattaacca gttgcgcaac ttcatcccat aacacataca accaacgccg ccagatgaac
     8341 gactccgcca ctggcgcgcg ttgcaggtaa ctccagagca aatcttccgc cagagaatta
     8401 tccattagtc ggaacagttc gtattctcgc ggtgcccaaa gcattagtcc cggcccgacc
     8461 atcgccagta actgatcgct gcgcgaatct ttcagcatgc tgcgtaaaca gaagttaccg
     8521 tgaatcaaca cacaattgtc gttaaagcct tcaaataatg ccgggagaca ttcgcgagtg
     8581 cgaaacagga tccgcttatc ctgcatcgtc agaccggtgt tattgaactg attgagcgtg
     8641 gtccatagca cttcaacatg ttgccggtac catgagggcc agaaattttc ctgagtattg
     8701 tcgaccgcgc cgacgcaacc gcgactgtcc tgacggtgcc aggccagtaa ggcttcaacg
     8761 atttggtctt tgagctgttc ccagcgttct ggtgttcggg ctggtgcctc caccgaaaca
     8821 ccacgcatcc gctccagtag caggacatca gggccggggt gttcttcatg cgtcatcaca
     8881 ccataaatag tcggcatacg gacagtccca cttcttgcca gcatggtggt tttccatgcc
     8941 agttgtcggg cttttccggg cgtactaaag cttcttgcca ttaacggcat tgggtttccc
     9001 tggctgtcat acaaagccca caacgccgta tccgcttttt cattgacgca ctcaatacga
     9061 ctgagttttt cgcccagtaa atggcttaat tcggcacgca gctgttccat tcagattacc
     9121 ctcataaaag ctactgcttt cataatgagc gccaatataa aagatgtcac ctgaatgaga
     9181 tcaattgttg aaaagaaaaa ttgaggaaaa tcgtaacggt acgacactcc tcccaaaatt
     9241 ggctgaagtg tcgtgaagtg aaattagcgc atttcagcgc gaacgcgctc aagatcttca
     9301 ggggtatcca cacctgtgcc aggaacttcc tgagcaacag caacatggat tttttcgccg
     9361 taccacagaa cacgaagctg ctctaacatt tcgatgtgtt ctaacggact tgcctgccag
     9421 ttgacgtaac gacggataaa gcctgcacgg tagccataaa taccaagatg acgcaggaag
     9481 ttatcgccaa cggtttcaag gccttctgca aaacgatcac gatcccaagg aatggtggca
     9541 cgtgagaagt acagtgcata cccttcagcg tcgagaacca ctttcaccgc attcgggtta
     9601 aacgcttctt ccgcattgtg gattggcacc gccagagtcg ccatacccac ctgacgctga
     9661 gcgaggttat cagcaacctg acgaatgatt gtcgcaggga tcatcggttc atcaccctgc
     9721 acattaacga tcaccgtgtc gtcgctgaat gcgcattttt cgacaacttc cgccagacgt
     9781 tctgttcctg actgatgatc ggcgcgcgtc atacatactt caccgccagc ggcttcaacg
     9841 gcgcgggcaa catcatcatg atcggttgcc acgatgatgc gctcggcacc tgattcacgc
     9901 gcgcgttcaa gaacatgaac aatcatgggt ttgccgttaa tatcaaccaa tggtttaccg
     9961 ggcagacgcg tggacgcgta gcgcgcggga ataatgacca caaaactcat gatttactct
    10021 catcagcagt cagcacgcgg gcttccgttt ccagcaacac tggaatgcca tcgcggagcg
    10081 ggaaagccag gttatccagt ttgcaaataa gctcttgttt ttcctggtta taccaaagtt
    10141 ttccgttgca aaccgggcag gcaatgattt caagcaaacg atgatccata gttcctccag
    10201 atggatcggg ttatgaatgc ataaatctta tcataatcat ttatgcgacg gggtctattt
    10261 cccagccagt gcggcaatca gtaaagagac cttgcgggca gcgtcctaat gtcacccgcg
    10321 ttgcctgctg ccagctcgcg aaatcagtaa tcgcctgacg taaccctttt tgcagcattg
    10381 tcgttggttt aatgccttcc tgtaaccaca gagagataac ttcaaggatg cctgtctggc
    10441 gatgcatttt ggcatccatt cgcccaacta attgcccacg atgtaataat ggcagaacaa
    10501 aatagccata ctggcgtttc ggcgctgggg tatagcactc cagccggtag ctaaaatcaa
    10561 aaagctgctc tgcgcgtttg cgatcccaga caacaggatc aaaaggcgaa agtaccgcgc
    10621 tgtgcgttgc agtgagcttt ccggccagcg cacgctcgag tagcggcagc aaatcagcat
    10681 gcagccaaag attgcccaat ttttcaacat gcacagcaat gatttgctgc tgttcagccc
    10741 tcgcttcgcg ccacgccgcc agtgccggac gtttcagccg atagtaatct gccagccact
    10801 gttcgcggaa tattcccagg ctacgcgcac tgttatccag catgatgatt tctgcttctg
    10861 tttgcgaaac gagatcgcgc tcatcatccc agtcaggcat gacacggtgg gttaaatcat
    10921 aaacgcgctg gaagttgcgc cgttcaatca ccatcacctt tccggcagta aataaacctt
    10981 ccagatgccg tttatgcggc ttccattccc accagccgct tgcaccttta cgaggatgct
    11041 caaaatcggc tgaacgtacc ggccctttat catgaatatg ctgaattaac tgtgcaattt
    11101 ccgcctcatg ttcctgcatc caggcgtctt tgtatttcca gcccattttt tcaggtgcca
    11161 gcatgcggtg gcgaataaga cgaaagtcgc tacgcggcat aaagcaggct tcatgcgccc
    11221 agtattccat taattcgcca cgcgccagag actcatccag ccactgggca ggataatttc
    11281 ccagacgact gaaaagcacc agatatggac tacgggcaac aatattgatg gtatcgattt
    11341 gcagcaagga catgcgggag atcgttgccg gaatatcctc caacgacgct cgacggcggg
    11401 gtttgtttaa caggccttgt gcggcaaggt gaagattacg cgcatcagca agggagaggt
    11461 gcggcagcga cattcatgac tccatcaatc gaacgctgcc gcggcgtaac tagttgccag
    11521 aagccagcga ggttagttgc gtaagcagtt tcgctggttc atcacctgaa agctgtgcgt
    11581 ctacaggcaa ataccaccaa ttttcttctg caaaggcccg gcatttcacc gcatcttttt
    11641 cagtcattac cagcgtttgc ccggcgctta ccaacgcact gacatccgca tggttcaaag
    11701 actgatgatc ggccagcggt acacattttt ccggttgtac gccacacatc ttcagcgtgg
    11761 caaaaaagcg cggcggatgc ccaatccccg ccatcgccac tacatgttca agctgagcaa
    11821 cgtcacaacg cgtaccggta cgtaaattca ccgcctgacc cggcagcaga tgcatgggga
    11881 tttcaccgct gcgagggaca ccgccgttga cgattaccgc atcaaccgac tttaagcgcc
    11941 ccgctcgctc acgcattggc cccgccggca accaccagcc attgccaaag cgacgcacac
    12001 catcaataac gacaatttcc acatcacgcg ccagacggta atgctgtaaa ccgtcgtcgg
    12061 ttacgatgat ctgcacatca gggtgttgcg ccagaatggc ttttaccgca tcagaacgaa
    12121 cgggagaaac cgcaacaggc gcatcagtgc gttgataaat caacacaggt tcatcacccg
    12181 cctgtgctgt ggtggtatct gccgacaata acagcggata agattcagcc ttaccaccat
    12241 atccccgcga tacgaccccc acgcgaatac cgcgctgttg caactgttcc accagccaga
    12301 caacgaccgg ggtttttccg ttgccgcctg cggtgagatt accaaccacg acaaccggta
    12361 cgggggcacg ccaggcgcgc ttcagtttta gtttatagca aagacggatc gcgccactca
    12421 ccaggccata caaccaggag agtggcagca atagccgcca caaaggggat tcaccagacc
    12481 agattttttc gatcattggc caaactgcat tttgtgaagt tgcgcgtaaa cgccgcggtg
    12541 ctcaagcaaa tcgttatgcg taccgcgttc cacaatgaca ccatcctcga cgaccacgat
    12601 ttcgtctgcc ttttcaatgg tagacaagcg gtgggcaatc accagagagg tacggttttt
    12661 ctgcaactca tccagtgccg cctgaatcgc acgttcggat tcggtatcca gagccgaggt
    12721 agcttcatcc agaatcagaa tcgggctatc acgcagcaag gctcgagcga tagcaatacg
    12781 ctgacgctga ccgccagaga gcagcacgcc gttttcacca atcactgtat cgagaccgtt
    12841 atccatctta ttgatgaagt ccatggcgta ggccatccgc gccgcttctt caatttgctc
    12901 acggctgtac tgttcagtcc gtgcgtaagc aatgttgtta gcaaccgtat cgttaaacag
    12961 atggacattc tgcgacacca gagcaacctg gttacgtaac gacgccaggg tatactcgcg
    13021 cagatcgtga ccatccatca ggatttcgcc ttcatcaata tcgtaaaaac gcgtgatcag
    13081 gctggcgatg gttgatttac ctgaaccaga gcgtccaacc agagcaaccg tcttccctgc
    13141 cggaattttc aggttgatgt tacgcaatgc gggtacgtca cgtcccggat aagtaaaggt
    13201 gacattgcgg aattccacgt cgccagtcgc acgctcgatc acgcgcttac cttcgtcttt
    13261 ctcctgctca ctgtccagaa tggtaaacag cgtctgacaa gccgccatac cgcgctggaa
    13321 ctgggcgtta acgttggtca gcgatttcag cggacgcatc agtgcaatca ttgaagagaa
    13381 aacaacggta atcgtaccgg cagtcaggtt atccataaca cttgggaagc tcgccgcata
    13441 cagaacaaac gccagcgcca aagaggcgat cagctgaatg atcggatcag agatggaaga
    13501 ggctgaaacc attttcatcc cctgaagacg cattcggtta ctgactttat cgaagcgttt
    13561 cgtttccact tcctgaccac cgaaaatcaa tacttctttg tggcccttca gcatttgttc
    13621 tgcactggtg gtcacttgcc ccatggtgtt ctgcatgttt ttactgatgt tgcgaaaacg
    13681 cttcgatact acgcgaatcg caatcgaaac aatcggtgcc agcacaatca aaatgatcga
    13741 cagttgccaa ctgtaataga acatcatgat gaacaggccg atgatcgacg caccttcacg
    13801 cacaacagta atcagtgcgc cggaagaaga agaagcaacc tgttcggaat cgtaggtgat
    13861 acgtgacaac agcgtacccg ttgactgttt gtcaaagaat gaaactggca ttcccatcat
    13921 gtgaccaaac aggcggcgac gcatggtcat taccaccttt cctgataccc aggagataca
    13981 gtagctggag acatagctgg tgataccacg taaaatcatc agtccgatca ccaccagcgg
    14041 catccacacc agcacggagc gatctgtttt accaaagcca tcatcaagaa gtggcttaag
    14101 gagcgataac atgaaggtat cgctggctgc gttgaggatt aacgctacgc ccgccacgat
    14161 cagacccgct ttgaaaggcg caatggttgg ccacagtcgg cggaatgtct gccacgtaga
    14221 gagatctttg tcgttatgca ttcaaaaaac cagcatttgt tgaaatagcc gcatattcta
    14281 cccgttatcc actggcacgc caaaccactg atgataccaa cgaggtaaaa tttgctctct
    14341 gaggctgcta atccgccagc cttgtgctga aaaattgacc gtcacttgcc cctgatgtgg
    14401 agtatcaagc cattgatatc ctttctgttg atagcgatgc ttaactttgt tagagggcaa
    14461 tcgccatgcg ttatagcgcg atgccgatgc gagtgccact tttccattca ctcgctgaat
    14521 taatggcaac gatgatgagg tattactgcc atggtgaggt acctgaagca atgttgcctg
    14581 cacttgctgc cagtaacggc ttagcatctt ttgttcagct ggggcttcaa tatcaccggt
    14641 tagaagaatg ctattcgtcc cgtcatcaac cttaaccaca caggaatggt tatttccctt
    14701 atcgttgcta ccttgtaaag gccagtgcgc gctgaaacgc aatccttgcc actgccacgc
    14761 ttcgccacgc acacagggct gatgatgttc ccagtttaac ggacttctga tccataacat
    14821 cggccatata tgcaatattg aatccagccc tccccggtga tccagatgtt catggctcag
    14881 aataacgcct tccggttcaa gattatgcca gtggagccag gggataatca gttgttgccc
    14941 actatcccct tcaggccagt cctgtgtcat agagaatcgc tttgccgttt ctggctatca
    15001 ccattgccag cccttgcccg acatcaagca tgtacagctg ccactcgtca ggtcgaggtt
    15061 tttgccacag cggccagcac atcagcaagc ctccagccac acacattgct ggcaacgttc
    15121 gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat agccattgcc
    15181 aacattcaac aatgttgatc cacccttccg gcaaactctt taacccccag aaaagtaaag
    15241 ccaaagaccg gtcggcaaga aaccataacc cctgttccag gattaacggc ccgctaaaat
    15301 gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag ggaattgcaa
    15361 atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt tgcatgggca
    15421 taagcaggag tgtgattccc agttgcagat ggatgaggga aacaactgca cgcaataccg
    15481 gcggcagttg ccactcagga cagggaaacc actgatacca aaaaatcagt gccgcgaccg
    15541 cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc agcaaaattg
    15601 ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt aacttaagca
    15661 ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt cccgtcaacc
    15721 aggcataaaa agcagcacag cagattccgc caattaatgg tatttgccag tggatccagc
    15781 gcccaggcag aaaaatttgt ccactgcgaa tgagtccggc agccagcaac gccgcaaaag
    15841 cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcgcgc atgatatttt
    15901 tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata accgcattcc
    15961 acggataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc aggctacaat
    16021 tcggttcaat gacacttgcc tgcagaaaac ggccggtgag cggctgatgc tgggcaatgg
    16081 cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca cgaactttga
    16141 gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagttattcg ccatacagca
    16201 caaggcctgg cgcagggaat atacgtcgac cttgtagata agtaatttga ccgtaatgag
    16261 tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg ccagttaagg
    16321 tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa aacagcaacg
    16381 tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag gcgaagagag
    16441 tcagaaacgc aagggttaat gtcctaggca attggggcaa aatcagcaac ggaaaaattc
    16501 cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg tttatggaaa
    16561 gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga ggcacattca
    16621 gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct tccagttcct
    16681 gattagagaa ttgaaaaaag cacccgacag gtgcttttct cgcgttcaag tttgagtaaa
    16741 aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa agtgaggaac
    16801 gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg tacgtggtgc
    16861 gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc cctgcgcaag
    16921 agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct tggcgggaat
    16981 gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga ttcctccggt
    17041 tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg ccgtagccgc
    17101 ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca acaaacttcg
    17161 gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga aagcttcagc
    17221 cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga ttgcatcttt
    17281 ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt tacgatcaac
    17341 gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag cgtcttcaac
    17401 gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag ccagttctac
    17461 ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc ctttcttgtt
    17521 cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca gggagatacg
    17581 ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt ttttgtattc
    17641 acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa ccaggccgtc
    17701 gatgccgccg tccaggccga tgaagatacc gaagtcagtg atagacttga ttttaccttc
    17761 aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag ctttgcactg
    17821 tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca taacttccac
    17881 tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc aatccatttc
    17941 ggaaacgtgt accagacctt caacgccttc ttcgatttca acgaagcagc cgtagtcagt
    18001 caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag cgatagctac
    18061 ccacggatct tcgcccagct gtttcaggcc cagggataca cgggtacgtt cgcggtcgaa
    18121 cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg gatgcttaac
    18181 gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca gatcaacgaa
    18241 tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt cctgcaggtt
    18301 ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac gacgagaaac
    18361 aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct ctttgccttc
    18421 caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca ggaacgcacg
    18481 aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa caccggtaac
    18541 agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac gtttagcttt
    18601 ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag caacgtcaac
    18661 ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct ctgccgggat
    18721 ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga tagcaacaac
    18781 aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact cttcaaagag
    18841 ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc cacctggctc
    18901 catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg gggtactgca
    18961 aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc tttttcaatc
    19021 aattgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc cggaaccagt
    19081 ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc caaaaggcgc
    19141 tcgaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg cgcacgttct
    19201 tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac cgttcccatg
    19261 tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg acgcaataat
    19321 gcttcacgaa cgcgtgggaa tgccgcgact tgtgaagccg cattcgccac ttcctgagta
    19381 cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc attggtcgac
    19441 acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttccga cgcaacatca
    19501 acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga gtccagcaga
    19561 tgccattgca acgcttccgc catagcctta cacaaggtgc ctttccctgc accgcttggg
    19621 ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa acaggcgtac
    19681 cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac gttgtgtgca
    19741 aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc gggctggcag
    19801 aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat cacggatgtg
    19861 ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc gaccgccatc
    19921 caggctagcc agtttttcaa agctgccaac cagcccctgg gtgctgatgc cacgtttctt
    19981 caacagatca aaagagaaat catctgcttc ggactcttta tcacgggagt atttagcatt
    20041 aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag ccacaccgct
    20101 ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaagctttca tttcagccag
    20161 cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa tttcgttgtc
    20221 attcatcatg tccatcaggc cactgtagac acgaacacag ccgttcgcca tcgcccatgc
    20281 gttgacgtcg ctggtcatat aaaccttata gttgaccggc gtgccattaa tattgttacc
    20341 caatgctttg gcgatttttg ccagacgttt accgtacttg ctggatttac ttgcgacttg
    20401 attgccgctg tccatttcgg cacagccctg attggcaatc gcttttgcat cggcatcgct
    20461 taatgttgct gctttatagg catttaaacc ggagctgata gccatattgg tatcaatacc
    20521 gtgggtattt tgacaccctg tcagtaatgc tgcagaggtc gcaatcgcca gcagtaattt
    20581 agtattcttc atgtgaatca tccgttgatt aatcatatat aaatattatg tatcaacaac
    20641 ttacattgac aagcgacgct accattaatc aaaatgactc acaaggtccg aaaaaaaacg
    20701 ccgacaatgt gc
//
LOCUS       DAETQL010000071        20691 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.071, whole genome shotgun sequence.
ACCESSION   DAETQL010000071 DAETQL010000000
VERSION     DAETQL010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.071"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            81..350
                     /locus_tag="K0W65_002849"
     CDS             81..350
                     /locus_tag="K0W65_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBI3096043.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="K0W65_002850"
     CDS             344..709
                     /locus_tag="K0W65_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBI3096044.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="K0W65_002851"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="K0W65_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBI3096045.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="K0W65_002852"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="K0W65_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBI3096046.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="K0W65_002853"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="K0W65_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBI3096047.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="K0W65_002854"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="K0W65_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBI3096048.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="K0W65_002855"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="K0W65_002855"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBI3096049.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="K0W65_002856"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="K0W65_002856"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBI3096050.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEVPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="K0W65_002857"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="K0W65_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="K0W65_002858"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="K0W65_002858"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HBI3096051.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="K0W65_002859"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="K0W65_002859"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HBI3096052.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14849..16447
                     /gene="rtcR"
                     /locus_tag="K0W65_002860"
     CDS             14849..16447
                     /gene="rtcR"
                     /locus_tag="K0W65_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HBI3096053.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16429..17187)
                     /locus_tag="K0W65_002861"
     CDS             complement(16429..17187)
                     /locus_tag="K0W65_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBI3096054.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="K0W65_002862"
     CDS             complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="K0W65_002862"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBI3096055.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="K0W65_002863"
     CDS             complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="K0W65_002863"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBI3096056.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18595..20100
                     /gene="glpD"
                     /locus_tag="K0W65_002864"
     CDS             18595..20100
                     /gene="glpD"
                     /locus_tag="K0W65_002864"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBI3096057.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20369..20599)
                     /locus_tag="K0W65_002865"
                     /pseudo
     CDS             complement(20369..20599)
                     /locus_tag="K0W65_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4893 a         5694 c         5213 g         4891 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgcat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct catggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aagtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggcggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggca cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cacttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctagt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttatt gtttctaaaa
    14761 agattatatc tttatatccg taaacggaga tttcccgcaa agccgattta ccgataatga
    14821 aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg gctttgtcgg
    14881 taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc gtccgtcact
    14941 ctgtatatac cagcaagaat cgttggtcat cgatcgactg gaattattgc acgatgcccg
    15001 ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc cagaaacaga
    15061 agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg tttacgcctg
    15121 cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact atttaattca
    15181 catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg aagcgcgtta
    15241 tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc cgcgcggccc
    15301 aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg ccagccgctt
    15361 tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca cgcgtaaccc
    15421 ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc gcgcgccgat
    15481 tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca tcttcgagtt
    15541 aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg ccaccctgcg
    15601 cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa ggcgcgttta ccggggcgcg
    15661 ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc ttgatgagat
    15721 tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag agaaaacctt
    15781 ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg ccggaacggt
    15841 gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt acgcgcggat
    15901 caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata ttgaaccgaa
    15961 cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc gttttaacac
    16021 cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggcaacat ggcgcggtaa
    16081 ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta gcggacgcat
    16141 cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc aggagagccg
    16201 cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc tcttcgaccg
    16261 tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt ctgctgccgg
    16321 acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg cggatcggct
    16381 acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc gtgcaggatc agcacagctc
    16441 cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg gggcgtcggt
    16501 gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac ggccaaattt
    16561 tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc gcttggtacg
    16621 aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc tgatccccag
    16681 aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc caatgatccc
    16741 gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt cttttaccat
    16801 caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt gattgagcag
    16861 cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg agccattggg
    16921 gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg tggctttacg
    16981 atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat gatggcgcag
    17041 gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg ggctgacgga
    17101 gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca gttcgataat
    17161 accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt cgttttcgcg
    17221 cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg atgtgtgctc
    17281 cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag atcagcgcaa
    17341 agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg ccacgtagcc
    17401 agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac ggcccgctga
    17461 atttttgctg catatagccg cttaacaggg cgctgataag cgtaatgaca attagcttac
    17521 cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc aggttaaaga
    17581 ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag aactcaaatt
    17641 tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg ccgagaattt
    17701 gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc ggacctgcgc
    17761 gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg ccggtatggc
    17821 ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga aaacgcgcca
    17881 gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg ctttggttat
    17941 gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac gcctgcgcca
    18001 cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat tccacacaaa
    18061 aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact gtcgctgcca
    18121 ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca gcagatactg
    18181 cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag tgtcaaagtc
    18241 gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct gcaccgcatg
    18301 ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt tttcctgcaa
    18361 cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc tctctttctt
    18421 tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat aaacgccata
    18481 atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg attctttgct
    18541 aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg cagcatggaa
    18601 accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc ggcagacgcc
    18661 gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg cgcgacctct
    18721 tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta tgaattccgc
    18781 ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc gcatatcgcc
    18841 ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg gatgattcgc
    18901 attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg atcaactggt
    18961 ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt cgaatattct
    19021 gactgttggg tagacgacgc ccgtctggta ctcgccaacg cccagatggt ggtgcgtaaa
    19081 ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa cggcctgtgg
    19141 attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc gcgcggcctg
    19201 gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca tctgccttcg
    19261 ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt gcatacccaa
    19321 aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat cccgtggatg
    19381 gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc gaaagcagtg
    19441 aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca ctttaaaaag
    19501 cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct gtgtgatgat
    19561 gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca tgatgaaaac
    19621 ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg aaaactggca
    19681 gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc gtggacgaaa
    19741 gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc cgctcgcctg
    19801 cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacacgcg cacttacggc
    19861 agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg ggaagatttc
    19921 ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg ggtccgccgc
    19981 accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc ggatcaacaa
    20041 tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct ggcgtcgtaa
    20101 attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga caaacacaaa
    20161 actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat tgaatttgca
    20221 tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa gcgcactttg
    20281 tcaaaaatct aacctacttt ttaattcagg aaattaccgc aaagcccacg ccatcatacc
    20341 caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc attaatgcgc
    20401 ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt tggcgtaaag agtaaattgc
    20461 aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct gcaaaaaatt
    20521 gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc cattttctat
    20581 tttgcattat tctttccatt ctttctgaat ggcgaaatta tactccccga gtccccttgc
    20641 cccttctgga cacttttccg aaatgatggc gaaaaaaacg ggaccctttg g
//
LOCUS       DAETQL010000072        20523 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.072, whole genome shotgun sequence.
ACCESSION   DAETQL010000072 DAETQL010000000
VERSION     DAETQL010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.072"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            245..586
                     /gene="raiA"
                     /locus_tag="K0W65_002866"
     CDS             245..586
                     /gene="raiA"
                     /locus_tag="K0W65_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBI3096058.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    689..814
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            690..737
                     /gene="pheL"
                     /locus_tag="K0W65_002867"
     CDS             690..737
                     /gene="pheL"
                     /locus_tag="K0W65_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HBI3096059.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            836..1996
                     /gene="pheA"
                     /locus_tag="K0W65_002868"
     CDS             836..1996
                     /gene="pheA"
                     /locus_tag="K0W65_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBI3096060.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="K0W65_002869"
     CDS             complement(2039..3160)
                     /gene="tyrA"
                     /locus_tag="K0W65_002869"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBI3096061.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="K0W65_002870"
     CDS             complement(3171..4241)
                     /gene="aroF"
                     /locus_tag="K0W65_002870"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBI3096062.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4451..4816
                     /locus_tag="K0W65_002871"
     CDS             4451..4816
                     /locus_tag="K0W65_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBI3096063.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4966..5484
                     /locus_tag="K0W65_002872"
     CDS             4966..5484
                     /locus_tag="K0W65_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBI3096064.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5474..6699
                     /gene="dgcN"
                     /locus_tag="K0W65_002873"
                     /pseudo
     CDS             5474..6699
                     /gene="dgcN"
                     /locus_tag="K0W65_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6715..7197
                     /locus_tag="K0W65_002874"
     CDS             6715..7197
                     /locus_tag="K0W65_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBI3096065.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="K0W65_002875"
     CDS             complement(7274..7621)
                     /gene="rplS"
                     /locus_tag="K0W65_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBI3096066.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="K0W65_002876"
     CDS             complement(7663..8430)
                     /gene="trmD"
                     /locus_tag="K0W65_002876"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBI3096067.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="K0W65_002877"
     CDS             complement(8461..9009)
                     /gene="rimM"
                     /locus_tag="K0W65_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBI3096068.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="K0W65_002878"
     CDS             complement(9028..9276)
                     /gene="rpsP"
                     /locus_tag="K0W65_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBI3096069.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="K0W65_002879"
     CDS             complement(9413..10774)
                     /gene="ffh"
                     /locus_tag="K0W65_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBI3096070.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10941..11732
                     /locus_tag="K0W65_002880"
     CDS             10941..11732
                     /locus_tag="K0W65_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBI3096071.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11753..13039
                     /locus_tag="K0W65_002881"
     CDS             11753..13039
                     /locus_tag="K0W65_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBI3096072.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="K0W65_002882"
     CDS             complement(13094..13687)
                     /gene="grpE"
                     /locus_tag="K0W65_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBI3096073.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13810..14688
                     /gene="nadK"
                     /locus_tag="K0W65_002883"
     CDS             13810..14688
                     /gene="nadK"
                     /locus_tag="K0W65_002883"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBI3096074.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14774..16435
                     /gene="recN"
                     /locus_tag="K0W65_002884"
     CDS             14774..16435
                     /gene="recN"
                     /locus_tag="K0W65_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBI3096075.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16584..16925
                     /gene="bamE"
                     /locus_tag="K0W65_002885"
     CDS             16584..16925
                     /gene="bamE"
                     /locus_tag="K0W65_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBI3096076.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16987..17277)
                     /locus_tag="K0W65_002886"
     CDS             complement(16987..17277)
                     /locus_tag="K0W65_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBI3096077.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="K0W65_002887"
     CDS             complement(17267..17704)
                     /gene="ratA"
                     /locus_tag="K0W65_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBI3096078.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17875..18357
                     /gene="smpB"
                     /locus_tag="K0W65_002888"
     CDS             17875..18357
                     /gene="smpB"
                     /locus_tag="K0W65_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBI3096079.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18572..18934
                     /gene="ssrA"
                     /locus_tag="K0W65_002889"
     tmRNA           18572..18934
                     /gene="ssrA"
                     /locus_tag="K0W65_002889"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19139..20380
                     /locus_tag="K0W65_002890"
     CDS             19139..20380
                     /locus_tag="K0W65_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBI3096080.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQLMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5300 a         5359 c         4815 g         5049 t
ORIGIN      
        1 cccttgagct gccgtttttt tattctgtta gttgtgaaac tgaagcgatt tagtcactat
       61 cgatctcatc aaatatggct cgctttgaga tattcctcaa gtaaaaaaac acctcttcct
      121 gcgatttctc acaaaaaaga ttcgttgaca aaaagtgaca aaattatgag atttccatca
      181 cacattttga catcaggaac ggtatgctga attcaccaag acgggaagac aagaggtaaa
      241 atttatgaca atgaacatta ccagcaaaca aatggaaatt actccggcca tccgccaaca
      301 tgtcgcagac cgtctcgcca aactggaaaa atggcaaaca catctgatta atccacatat
      361 cattctgtcc aaagagccac aagggtttgt tgctgacgcc acaatcaata cacctaacgg
      421 cgttctggtt gccagtggta aacatgaaga tatgtacacc gcaattaacg aattgatcaa
      481 caagctggaa cggcagctca ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac
      541 atcggtgaaa gacgccaact tcgtcgaaga agttgaagaa gagtagtcct ttatattgag
      601 tgtatcgcca acgcgccttc gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta
      661 ctgtactaaa gtcacttaag gaaacaaaca tgaaacacat accgtttttc ttcgcattct
      721 tttttacctt cccctgaatg ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac
      781 aataaggcct cccaaatcgg ggggcctttt ttattgataa caaaaaggca acactatgac
      841 atcggaaaac ccgttactgg cgctgcgaga gaaaatcagc gcgctggatg aaaaattatt
      901 agcgttactg gcagaacggc gcgaactggc cgtcgaggtg ggaaaagcca aactgctctc
      961 gcatcgcccg gtacgtgata ttgatcgtga acgcgatttg ctggaaagat taattacgct
     1021 cggtaaagcg caccatctgg acgcccatta cattactcgc ctgttccagc tcatcattga
     1081 agattccgta ttaactcagc aggctttgct ccaacaacat ctcaataaaa ttaatccgca
     1141 ctcagcacgc atcgcttttc tcggccccaa aggttcttat tcccatcttg cggcgcgcca
     1201 gtatgctgcc cgtcactttg agcaattcat tgaaagtggc tgcgccaaat ttgccgatat
     1261 ttttaatcag gtggaaaccg gccaggccga ctatgccgtc gtaccgattg aaaataccag
     1321 ctccggtgcc ataaacgacg tttacgatct gctgcaacat accagcttgt cgattgttgg
     1381 cgagatgacg ttaactatcg accattgttt gttggtctcc ggcactactg atttatccac
     1441 catcaatacg gtctacagcc atccgcagcc attccagcaa tgcagcaaat tccttaatcg
     1501 ttatccgcac tggaagattg aatataccga aagtacgtct gcggcaatgg aaaaggttgc
     1561 acaggcaaaa tcaccgcatg ttgctgcgtt gggaagcgaa gctggcggca ctttgtacgg
     1621 tttgcaggta ctggagcgta ttgaagcaaa tcagcgacaa aacttcaccc gatttgtggt
     1681 gttggcgcgt aaagccatta acgtgtctga tcaggttccg gcgaaaacca cgttgttaat
     1741 ggcgaccggg caacaagccg gtgcgttggt tgaagcgttg ctggtactgc gcaaccacaa
     1801 tctgattatg acccgtctgg aatcacgccc gattcacggt aatccatggg aagagatgtt
     1861 ctatctggat attcaggcta atcttgaatc agcggaaatg caaaaagcat tgaaagagtt
     1921 aggggaaatc acccgttcaa tgaaggtatt gggctgttac ccaagtgaga acgtagtgcc
     1981 tgttgatcca acctgatgaa aaggtgccgg atgatgtgaa tcatccggca ctggattatt
     2041 actggcgatt gtcattcgcc tgacgcaata acacgcggct ttcactctga aaacgctgtg
     2101 cgtaatcgcc gaaccagtgc tccaccttgc ggaaactgtc aataaacgcc tgcttatcgc
     2161 cctgctccag caactcaatc gcctcgccga aacgcttata gtaacgtttg attaacgcca
     2221 gattacgctc tgacgacata atgatgtcgg cataaagctg cggatcctga gcaaacagtc
     2281 gcccgaccat cgccagctca aggcggtaaa tcggcgaaga gagcgccaga agttgctcaa
     2341 gctgaacatt ttcttctgcc aggtgcagcc cgtaagcaaa agtagcaaag tggcgcagag
     2401 cctgaataaa cgccatattc tgatcgtgct cgacagcgct aatacgatgc aaccgagcgc
     2461 cccagacctg aatttgctcc agaaaccatt ggtatgcttc cggcttacgt ccatcacacc
     2521 agaccacaac ttgctttgcc aggctaccgc tgtccgggcc gaacatcggg tgtaacccca
     2581 gtaccgggcc atcgtgcgcc gccagcatgg cctgtaatgg tccatttttc actgatgcca
     2641 gatcaaccag aatacaatct ttcggtaaag gcggtaattt gccgataact tgctcagtaa
     2701 cgtggattgg cacactaaca atcaccattc cggcatcggc aacaatatca gccgctcgat
     2761 cccagtcatg ttgctccaga atccgcacct gataacccga tagtgtcagc atcttctcga
     2821 acaggcgtcc catctgaccg ccaccgccga cgataaccac cggacgcagt gacggacaaa
     2881 gtgttttaaa tcctttgtcg ttttcactgg agtaagattc acgcatcacc cgacgcaaaa
     2941 catcctcaat cagatctggc ggtacaccca gagcttccgc ctctgcacga cgcgaggcca
     3001 acatagatgc ctcgcgctcc ggaacataaa taggcagtcc aaagcggctt ttcaccttgc
     3061 ccacttcagc aaccagttcc agacgcttcg ctaataaatt cagcagcgct ttatcgactt
     3121 catcaatttg atcgcgtaat gcggtcaatt cagcaaccat aataaacctc ttaagccacg
     3181 cgagccgtca gctgcccgtt cagatcctga tgaatttcac gcagcaaggc atcggtcatt
     3241 tcccagctaa tgcaggcatc ggttacggat acaccgtatt tcatttcact gcgcggttgc
     3301 tcagaagact gattgccctc gtggatatta ctttcgatca tcagaccaat aattgagcga
     3361 ttaccatctt tgatttgagc aaccacggat tctgccaccg caggctgacg gcgataatct
     3421 ttattggaat taccgtggct gcaatctacc atcagagacg ggcgcagtcc cgcctgttcc
     3481 atctcttttt cacattgcgc aacatccgca gggctatagt tcggcgcttt accaccgcgc
     3541 aggatcacat gcccgtccgg attcccctga gtttgtagca acgcaacctg ccctgcctgg
     3601 ttaatgccaa caaaacagtg cggctgggcg gcggcgcgca tagcgttaat tgctgttgcc
     3661 agactgccgt cggtgccgtt tttaaaacca accggcatgg aaagcccgga ggccatttca
     3721 cggtgagttt gcgattccgt tgtacgagca ccaattgctg accagctaaa cagatcgccc
     3781 aggtattgcg ggctattcgg atctaacgct tccgtcgcca gtggcagtcc catattcacc
     3841 agctcaagca acaatttacg cgcgatttgc agcccggctt ctacatcaaa agagccatcc
     3901 atatggggat cgttaattaa ccctttccag ccgacagtgg tacggggttt ttcaaaatag
     3961 acgcgcatta ccagatagag gctatcgctg acctctgcgg caagagcttt aaatcgacga
     4021 gcatattcca gagcagtttc cggatcatga atggaacaag gaccacatac taccagcaga
     4081 cgaggatcgc gcccggcgat aatatctgaa atggttttac gcgagtcagc aatctgggct
     4141 tcttgttgca ggctcaatgg aaaagcggcc ttcagttgtt ccggagtcat taaaacctgt
     4201 tcgtcggtaa tatgtacgtt attcagcgcg tctttatgca tgatggcgat cctgtttatg
     4261 ctcgtttgcg atagttgctc ctcagcgagg atgacgtaac gataacacat aaagtaaagt
     4321 tttcaatcca tatttcgtac atttttattt acacaggcaa tttagtcgcg ctttcaaccc
     4381 ttgcttctgt atagataaat ttacaccccc tttaaaaaca atccgctatg ctttgaaaaa
     4441 ggagaaagaa atgatgaaaa agtttatcgc ccccttgttg gctttactgg ttagcggatg
     4501 tcagattgat ccttatactc acgcgccaac cttgaccagc accgactggt atgatgtcgg
     4561 tatggaagat gcgatatcgg gcagcgccat aaaagatgac gatgcattta gcgattcaca
     4621 ggcggatcgc ggtctatacc ttaaaggata tgccgaaggg caaaagaaaa cttgccagac
     4681 cgattttact tatgcccggg gactttccgg taaaagcttt cctgccagct gcaacaatgt
     4741 tgaaaatgcc agccaactgc atgaagtatg gcaaaaaagg gctgatgaaa atgccagcac
     4801 aatacgatta aattaattaa aaccccacaa ataaattaaa cataagattt tactcattgt
     4861 gttttatttc tcacattgat gacggtcgca tgaaaaaatg atattttaat gagcatcctg
     4921 aaaaaatatc gatgcatttc gagcgaagat ggtggggatc cctgaatgcg tttttctcac
     4981 cgactgttct tacttctgat tctcctgctg acgggagcac caatactcgc tcaagagcct
     5041 tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta cacccgctgg
     5101 cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacgttt tagtaccgcc
     5161 ctacaagaga atgccgcgac atctttgcct tatctgcctg tcatcattca cactcaacaa
     5221 gaggcgatga tttcaggctg caatggtttt tattttggca acgagtcacc gacattccag
     5281 atggaattaa ccgaacaata tccgtcaaag gcgttattat taattgccga acaaaatacc
     5341 gagtgcatta ttggtagcgc cttttgtctg atcatccaca ataatgacgt cagatttgcc
     5401 gtaaacctgg atgcctcatc gcgtagcggt gtaaaggtca acccggatgt cttaatgctc
     5461 gcacggaaga aaaatgatgg ataacgataa ttctcttaat aagcgcccca cgtttaaaag
     5521 agcattacgc aacatcagta tgaccagcat atttatcact atgatgctga tctggttgct
     5581 gctttccgtg acctcggtgc tgaccctgaa acagtacgcg caaaaaaacc tggcactgac
     5641 agcagcaaca atgacttaca gtctggaagc agctgtcgtt tttgccgatg gccctgcagc
     5701 aactgaaaca ctggcagcgc tgggccagca agggcatttt caactgcaga agtacgtgat
     5761 aagcagcaaa atattctggc atcctggcat tacccccgta aggatccagg cgatactttc
     5821 agcaatttca taagccactg gctcttccct gcccccatca ttcagccgat tcgtcacaat
     5881 ggtgaaacca ttggcgaagt acgcttaacc gctcgcgaca gttcaatcag ccattttatc
     5941 tggttttcgc tcgccgtact gaccggttgt attctgctgg catcaggcat cgcaattacc
     6001 ctcacccgcc atttgcacaa tggcctggtg gaagcactga aaaatatcac cgatgtcgta
     6061 catgatgtgc gttccaaccg caatttttcc cgacgagttt cggaagaacg tatcgctgag
     6121 tttcaccgct tcgctctcga cttcaacagt ctgctggatg aaatggaaga gtggcagctt
     6181 cgtttacagg ccaaaaatgc gcagcttctg cgtaccgcgc tacatgaccc attaaccggg
     6241 ctggctaacc gcgcagcgtt tcgtagcggc atcaatacgt tgatgaacaa ttccgatgcc
     6301 cgaaaaacgt cggcgttact atttcttgat ggcgataatt tcaaatatat caatgatacc
     6361 tggggtcatg cgacgggcga tagggtcttg attgaaatcg caaaacggtt agctgaattt
     6421 ggcgggctgc gacataaagc ataccgcctg ggcggcgatg aattcgctat ggtgctctat
     6481 gatgtacagt cggaatctga agtgcagcag atatgctcag cactgacaca aatctttaat
     6541 ctcccgtttg atcttcataa tggccatcag accaccatga cattaagcat tggttacgcg
     6601 atgaccattg agcacgcctc tgcggaaaaa ttacaagagc ttgccgatca caatatgtat
     6661 caggccaaac accagcgtgc cgaaaagctg gtgagataac aaggatatat cgatatgata
     6721 aagcacctgg tagcacccct gattttcacc tcactaatac tgactggctg ccagtcccct
     6781 cagggaaagt ttactcctga gcaagtcgcc gctatgcaat cttatggatt tactgaatcc
     6841 gccggcgact ggtcgctggg cttatcagat gccattctgt tcgcaaaaaa tgactacaaa
     6901 ttgctcccgg aaagccagca acagatccaa accatggcag ccaaattggc ctcgacaggg
     6961 ctaacacatg cccgtatgga tggacacacc gataactatg gtgaagacag ttacaacgaa
     7021 ggcttatcat tgaaacgggc gaatgtcgtg gccgatgcat gggctattgg tggacaaatt
     7081 ccacgcagca atctcaccac acagggttta ggaaaaaaat atcccatagc cagtaacaag
     7141 accgcccagg gccgcgccga gaaccgccgc gtcgcagtgg tgattgcaac cccttaaaac
     7201 acgagcaaac agacaaaaaa agggccagcc aattggccag cccttcttaa caggatgtcg
     7261 cttaagcgaa atcttagtta agacgctctt tgatacgagc agccttacca gtacgctcac
     7321 gcaggtagta cagtttagct ttacgaacag caccacgacg tttgacagaa atgctgtcaa
     7381 ctaccggaga gtgagtctgg aagacacgct caacgccttc gccgttggaa attttacgaa
     7441 cagtgaatgc agagtgcaga ccgcggttac gaatagcgat aaccacgccc tcgaatgcct
     7501 gcagacgttt tttggaacct tcaacaaccc atactttcac ttccacggta tcacccggac
     7561 ggaaggaagg tacgtcctgc ttcatctgct cttgttcaag ttgcttaata atgttgctca
     7621 taatttaatc tcttatcctg ggtaaactga tctctcgggg gcttacgcca tcccatcatg
     7681 tttatgttgc tgttgtgcgt gttccgtttt gaactccgcc agcaaccttg cttgctcttc
     7741 agtcagagcc aggttttcca gaagttcagg tcttctaagc caggtacggc ccagcgactg
     7801 tttcaaacgc cagcgacgta tctcggcatg gttgcccgac agtaacactg gcggaacttc
     7861 catcccttct aacacctcag gccgcgtata gtgcgggcaa tccagcaatc cttcagcaaa
     7921 ggaatcttcc gttgccgagg cttcatgtcc cagtactccc ggaataaacc gggaaacgga
     7981 gtcaatcagc gtcattgctg gtaactcacc accactgaga acgtaatcgc cgattgacca
     8041 ttcttcgtca atttcggttt ggatcacgcg ctcatctata ccttcgtagc gaccgcacac
     8101 cagaatcaat ttttgattcg ttgccagttc gctgacgccc gcttgatcaa gcttgcgtcc
     8161 ctgtggtgac agataaatca cctttgcgcc ttcacccgcc gcggcttttg ctgcatgaat
     8221 ggcgtcccgc aagggttgca ccatcattaa catccccggt ccgccgccgt aaggacgatc
     8281 gtccacggta cggtgccggt catgcgtgaa gtcgcgagga ctccaactct ggatgctcag
     8341 caggccattt ttaactgccc ggccagttac cccgtaatcg gtaattgcgc ggaacatttc
     8401 aggaaacagg ctaattatgc caatccacat agcgccgcct tttaccgttt atccggtggt
     8461 ttaaaaacca ggatcccaat ctacttcgat tgaacgagta gtgagatcga ctttcttgat
     8521 aacctgccca tcgaggaacg gtacgagacg ttccttgata ccaaacgcat ctttcaggtt
     8581 tgccttaatg acgagaacgt cattagatcc ggtttccatc atatcaacga ctttaccgag
     8641 atcgtagcct tcagtggtta ctacctggca gcccatcagg tctttccagt agtagtcccc
     8701 ctcttcaagc tgaggcagct gcgatgaatc cacgacaatt tcacaattcg tcagcaggtt
     8761 cgccgcatca cgatcgtcaa cgcctttcag cttgatgatc atgtcctgat tgtggtgctt
     8821 ccagctttcc agctggactt gctgccactg acccgccttc tggataaacc agggctgata
     8881 gtcaaaaatg ctttcggcgt cttcggtgga agaaaacact ctgagccacc cacgaatacc
     8941 gtaagacgaa cccatttttc ccaaaacgat gggatcaaca ggtgcttgcg cggtgagttg
     9001 tttgctcatc atgaccaccg tgacagatta agctgctttg tttacttctt tgatcagcgc
     9061 agcaacgcga tcagaaatag ttgcgccctg gccaacccag tgagcgatgc gatccagatc
     9121 caggcgagtg ccttcttctt tttcgctagc gattgggttg aagaaaccaa cgcgctcgat
     9181 gaagcgaccg ttgcgtgcat tacggctgtc agcgacaaca acctggtaga acggacgctt
     9241 tttagcgccg tgacgtgcta aacgaatagt taccataaca tcctcttgtg tgaataaaac
     9301 aaccggaccc catcgaggaa cggagtccgg tgtcatatta aaagcccgaa aattttactg
     9361 atttctggag aaaatgcaat cagcagttaa taactctgtc gcataaggtc atttagcgac
     9421 caggaaatcc cggcggcatc attcccttca tgcttctcat catcttcgcc attccgcctt
     9481 tcttcatttt cttcatcatg cgctgcatgt cgtcgaactg cttcagaagg cggttaacgt
     9541 cctgtacctg catcccgcaa ccggcggcga tacgacgctt acgcgaacct ttgatgattt
     9601 ctggcttagc gcgctctttc atcgtcattg agttgatgat ggcttccata cgcaccagca
     9661 ctttatcgtc catctgcgac ttgacgttat ccgggatctg ccccatgccc ggcagtttgc
     9721 ccatcaagct ggccatgccg cccatatttt tcatctggcg cagttgctca agaaagtcgt
     9781 tgagatcgaa gccgtcgcct ttttttagct tgctggctaa tttctctgcc tgcgcgcggt
     9841 caactttgct ttcgatatct tcgatcagcg acagtacgtc gcccatgccg agaatacgcg
     9901 acgcgatgcg gtccggatgg aacggctcca gcgcctcagt tttctcgcca acaccgagga
     9961 acttgatcgg tttgccagtg atgtgacgaa tagagagcgc cgcaccgccg cgggcatcgc
    10021 cgtccacttt ggtcaacact acgccggtaa gcggtaacgc ttcattgaat gcttttgccg
    10081 tattggccgc atcctgaccg gtcatggcgt caaccacaaa cagggtttca accgggttaa
    10141 tcgacgcatg gacttgtttg atctcgtcca tcatcgcttc gtcaacgtgc agacgaccag
    10201 cggtatccac cagcagcacg tcgtagaatt tcagtttggc ttctttcagc gccgcgttaa
    10261 caatatcaac cggcttctga ccaacatcag aagggaagaa atcaacgccc acctgctctg
    10321 ccagcgtctc aagctgtttg attgccgccg ggcgatagac gtcagcagaa accaccagca
    10381 ctttcttctt gtgcttctcg cgcaggaaat taccgagttt accaacgctg gttgttttac
    10441 cggcaccttg caggcctgcc atcagcacga ccgcaggcgg ttgcgcagcc agattcaggg
    10501 tctggttctc ttcgcccatc gccgcaacca gttcgttacg gacgattttg acgaactcct
    10561 gccccggcgt caggctctta ttaacttcat gaccaaccgc tttctctttt acgcgattga
    10621 taaactcacg cactaccggc agagcgacgt ccgcctccag cagcgccatg cgcacttcgc
    10681 gcagcgtatc ttttacgttg tcttcagtga ggcgtccacg gccactgata ttgcgcagcg
    10741 tgcgcgacaa acgatcggtt aaattatcaa acattgtctc tcgcctgggg tggaaacggt
    10801 tggccgcaat cgcgacacat catcagtatt ttgccgcagt ataacatgaa ggcgtctttg
    10861 ttgttatgca acggttggag cagcgttcac ctgacgctat actgcttctc tttcttattg
    10921 ctcaaactgt cgacattact atgcccgttt ttgctctgct cgcgcttgtc gcctactccg
    10981 tcagtcttgc gctgattgtt cccggtctgc tgcaaaaaaa cggcggctgg cggcgcatgg
    11041 ctattatttc tgcggtcatt gcgctggtct gccacgcaat cgctctggaa gcccgcatcc
    11101 tgcccgacgg tgatagcgga caaaacctca gcctgctgaa cgttggttca ttggtcagtt
    11161 tggtgatctg tacggtaatg accattgtgg cttctcgcaa tcgtggctgg ttgctgctac
    11221 ccattgtcta tgcctttgcg cttatcaacc tggcgctggc gaccttcatg cccaatgaat
    11281 acatcaccca tctggaagca acgcctggga tgctgatgca cattggctta tcgctctttt
    11341 cctatgccac gctaattatc gccgccctgt acgcgctgca actggcgtgg attgattacc
    11401 aactgaagaa caagaagctg gcatttaacc aagaaatgcc gccattgatg agtatcgagc
    11461 gtaaaatgtt ccacatcacg cagattggcg tggtgctgct aacgctcacg ctttgcactg
    11521 gcctgttcta catgcacaac ctatttagca tggaaaatat cgacaaggct gtgctctcta
    11581 tcgtggcgtg gtttgtctat attgtgctgc tgtggggaca ttaccatgaa ggatggcgtg
    11641 gacgccgcgt cgtctggttt aacgttgcgg gcgcggtaat tctgacactg gcctacttcg
    11701 gcagccgaat tgtccagcag ttaatcagct aaacccagaa aaggagtttc ccctggaaca
    11761 catttctact actacgttga tcattattct gatcatcatg gtggtcattt cagcctattt
    11821 ttccgggtcc gaaaccggaa tgatgaccct caaccgctat cgtcttcgac atatggcgaa
    11881 acagggtaat cgctcggcca aacgcgtcga aaaattgctg cgtaagccag accgcctgat
    11941 aagcctggtg ttaatcggca ataacctggt caatattctt gcctccgcgc tcggcactat
    12001 tgtcgggatg cgtttgtacg gcgatgcggg cgtggcaatt gcgactggtg tgctgacttt
    12061 tgtcgtactg gtttttgctg aggtattgcc gaaaaccatt gccgcgctgt acccggaaaa
    12121 agtcgcttat ccgagtagtt ttctgctggc tccgctgcaa attttgatga tgccgctggt
    12181 ctggttgctg aatgctatca cccgtatgct gatgcgcatg atgggtatca aaaccgatat
    12241 cgtggttagc ggctctttga gcaaagaaga gttgcgcact atcgtgcatg aatcgcgctc
    12301 acaaatttcc cgtcgcaatc aggatatgct gctgtcggtg ctcgatctgg aaaaaatgac
    12361 cgttgatgac atcatggtac cgcgcagtga aattatcggt attgatatca acgatgactg
    12421 gaaatctatt ctgcgccaac tctcccactc acctcacggg cgcatcgtgc tttaccgtga
    12481 ttcgctggac gacgccatca gtatgctgcg agtacgtgaa gcctggcggt tgatgtcgga
    12541 gaaaaaagag ttcaccaaag aaaccatgct gcgcgccgcg gacgagatct attttgtgcc
    12601 tgaaggtacg ccgctcagca cgcagttggt aaagtttcag cgcaacaaaa agaaagtcgg
    12661 cctggtcgtc aacgagtatg gagacattca ggggctggtg acggttgaag atattctgga
    12721 agagattgtt ggcgatttca ctacgtcgat gtcgccaaca cttgccgaag aggtcacgcc
    12781 gcaaaacgac ggttcggtga ttatcgatgg caccgccaac gtgcgggaaa tcaacaaagc
    12841 ctttaactgg catctaccgg aagatgatgc ccgcacggtt aatggcgtca ttcttgaggc
    12901 actggaagag atccctgtcg caggcacccg cgtgcgtatt ggcaagtacg atatcgatat
    12961 tctcgacgta caggacaata tgattaagca ggtaaaagtt tttcctgtga aaccgctgcg
    13021 cgagagtgtg gcggagtaac gaaaacggcc ctgcattcgc atgcagggcc gtgaattatt
    13081 acgaaaacag aaattacgct tttgctttcg ctacagttac catcgccgca cgaatcgtac
    13141 gaccattcag cgtataaccc ttctgcataa tgcccagtac gttacctggc gcaacgtcat
    13201 cagattccac cattgcgata gcctgatgca cattcgggtc cagcggcacg ttagtttcgg
    13261 cgatcacttc aacaccaaac ttacgcacaa catccagcat cgacttcagc gtcagctcaa
    13321 tgccttcaac catcgcagac atatccgggt tagctttatc agccacttcc agcgcacgat
    13381 ccaggctatc aatcaccggc agcaattcgt tgatgaattt ctccagcgcg aatttgtggg
    13441 ctttttcaat atccagttca gtacgacgac gcaggttttc catttcggct tttacgcgca
    13501 aaataccgtc acgttcacgg gtctgggctt cagccagctg agcttcgaga ttcgcaattt
    13561 tttcatcgcg cggatccacc tgctcagcag aagcttctgg ctcaactgcc tcaatctctt
    13621 cgtgctgatc catgataatt tcttccgggg cttgcccctc aggcgttttc tgttctttac
    13681 tactcatgaa tttctccgcg tttttttcgc attcatctcg ctaacttcgc ttattatggg
    13741 gatcagtttc agggtttcaa gggaagcact cacattgtca tcaatcttcg caacaaggac
    13801 ctcggaaaaa tgaataatca tttcaagtgt attggcattg tgggacaccc acggcaccct
    13861 actgcactga caacacatga aatgctctac cgctggctgt gcacaaaagg ttacgaggtc
    13921 atcgttgagc aacaaatcgc tcacgaactg caactgaaga atgtgaaaac tggcacgctc
    13981 gcggagattg ggcaactggc tgatctcgcg gtagtcgttg gtggcgacgg taatatgctg
    14041 ggcgcggcgc gcacgctcgc ccgttacgat attaaagtta ttggaatcaa ccgtggcaac
    14101 ctgggtttcc tgactgacct tgaccccgat aacgcccagc aacaattagc cgatgtgctg
    14161 gaaggtcact acatcagcga gaaacgtttt ttgctggaag cgcaagtctg tcagcaagat
    14221 tgccaaaaac gcatcagcac cgcgattaac gaagtggtac ttcatcctgg caaagtggcg
    14281 catatgatag agttcgaagt gtatatcgac gagatctttg cgttttctca gcgatctgat
    14341 gggctaatta tttcgacgcc aacaggctcc accgcctatt ccctctctgc gggcggtcct
    14401 attctgaccc cctctctgga tgcgattacc ctggtgccca tgttcccgca tacgttgtca
    14461 gcacgaccac tggtcataaa cagcagcagc acgatccgtc tgcgtttttc gcatcgccgt
    14521 aacgacctgg aaatcagttg cgacagccag atagcactgc cgattcagga aggtgaagat
    14581 gtcctgattc gtcgctgtga ttaccatctg aatctgattc atccgaaaga ttacagttat
    14641 ttcaatacat taagcaccaa gctcggctgg tcaaaaaaat tattctaatt ttacgccagc
    14701 ctctttactg tatataaaac cagtttatac tgtacacaat aacagtaatg gtttttcata
    14761 caggaaaacg actatgttgg cacaactgac catcagcaac tttgctatcg ttcgtgagct
    14821 tgagattgat tttcatagcg gcatgaccgt aataactggc gagaccggtg cgggtaaatc
    14881 tattgcaata gatgccctcg gtctttgtct cggtggtcgc gctgaagccg acatggtgcg
    14941 taccggcgct gctcgcgctg acctgtgcgc ccgtttttct ctgaaagata cgccagcggc
    15001 cctgcgctgg ctggaagaaa accagcttga agacgggcat gaatgtttgc ttcgtcgcgt
    15061 gatcagcagt gatggtcgct cccgtggttt catcaacggt acagctgttc ctctgtcaca
    15121 actgcgcgaa ctgggtcagt tgctgattca gatccatggt cagcacgctc atcaattact
    15181 caccaaacct gagcaccaaa aattcctgct tgatggctat gccaatgaaa cctctctact
    15241 tcaggaaatg accgcacgtt atcagttgtg gcatcaaagc tgccgtgacc tcgcgcatca
    15301 tcaacagtta agtcaggaac gcgccgcccg tgcggaactg ctgcaatacc aattaaaaga
    15361 acttaacgaa tttaatccgc agcccggaga gtttgagcaa atcgacgaag agtacaaacg
    15421 tctggcgaac agcggtcaat tgctgaccac cagccagaat gcattggcat taatggccga
    15481 cggtgaagac gcaaacctgc aaagtcagct ttacacggct aaacaactgg tgagcgaatt
    15541 gattggcatg gacagcaaac tgtccggcgt acttgatatg ctggaagaag ctaccatcca
    15601 gattgctgaa gccagcgacg aactgcgcca ctactgcgat cgtctggatc tcgatcccaa
    15661 ccgactgttt gaacttgaac agcgcatctc aaaacagatt tcgctggcac gtaaacatca
    15721 cgtcagccct gaggcattac cacagtatta ccagtcgcta ctggaagaac agcagcaact
    15781 ggacgatcag gccgactcac aagaaacgct cgcgctggcg gtaacgaaac atcatcagca
    15841 ggcactggaa accgcgcgcg cattacacca acaacgccag caatatgcag aagaacttgc
    15901 acagctgatc accgacagta tgcatgcgct ctcaatgccg catgggcagt ttacgattga
    15961 tgtcaaatat gacgagcatc acctgggcgc tgacggtgcc gatcgtattg agtttcgggt
    16021 aaccaccaac ccaggtcagc caatgcagcc tattgccaaa gtcgcatccg gtggtgaatt
    16081 gtcccgcatc gcactggcaa tccaggtcat cacggcgcgt aaaatggaaa ccccggcact
    16141 gatttttgat gaagtggatg tagggattag cggtccaaca gcggcagttg tcggcaaact
    16201 gctgcgtcaa ctcggcgaat caactcaggt gatgtgtgtt acccacctgc cacaagtcgc
    16261 gggatgtggt catcaacact attttgtcag caaagaaacc gatggtgcga tgacagaaac
    16321 acatatgcaa tctctggata aaaaagcgcg gttacaagag ctggcgcgcc tgcttggtgg
    16381 cagtgaagtc acacgtaaca cactggcgaa tgcgaaagaa ctgcttgcag cgtaaacttt
    16441 tttcctgctt cacggtcaga gtaaacagca aaacgccgta agaccggaaa gcaaaaggtt
    16501 ttaaagtgat gaaaggtcta ttatcatcgg catattacag atgagccacg tactgctcgg
    16561 gcccgaaaag gaatcaaatc actatgcgct gtaaaacgct gactgctgca gcagcagtac
    16621 tattgatgtt aaccgcaggc tgttccactc tggagcgagt ggtttaccgt cctgacatca
    16681 accaggggaa ctatctgacc gctaacgacg tatccaaaat acgtgttggc atgacgcaac
    16741 aacaagttgc gtacgcattg ggtacaccgc tgatgtccga tccatttggt acgaatacct
    16801 ggttctatgt cttccgccag caaccaggtc atgaaggtgt aacgcagcaa acgctgacgc
    16861 tgacctttaa cagtagcggt gtgttgacca atattgataa caaacctgcg ctgagtggta
    16921 actaataata aagttgctct caaagacgtt aaaaaaggtg ctcaatgagc accttttttc
    16981 tgtctgttat ttattcgctg atttttctgc tcgttgcctg cgaagctctt tcggatcggc
    17041 aatgagaggg cgataaatct ccacccgatc gccatcatgc acactatcgc ttagttttgc
    17101 cggacggctg taaatgccga ctttgttttt agttaaatcg atatcggtac gcaattccag
    17161 caagccactg gcgcgaatag cttcttcaac cgtcgcgccc tcctgcagcg tcactcgctg
    17221 caggtattgc ttctcaggta gcgcataagc cacctcaacg gcaattttac ctagcactgt
    17281 aaacctcttt cgcacgaacc gtaaaagcct ggaccatatt agccgccagc tctttaaaca
    17341 cgcgaccaaa ggcgagttca atcaacttat tggtaaactc aaagtcgaga tgaaactcga
    17401 tacgacacgc ctcctggctc agcggcgtaa acttccatcc accaatcaat ttcttgaacg
    17461 gcccatccac cagattcata agaatacttt ggttactggt caactggttg cgggtagtaa
    17521 acgttttgct gatcccagcc ttagagacat ctaccgccgc agtcatctgc ccaggagtgg
    17581 actccagaat ccgacttccg gtacaacctg gcaaaaactg aggataagac tgaacgtcat
    17641 tcactaactg atacatttgc tccgcgctgt agggtaccag tgcggtccgg ctaatctgag
    17701 gcataacaat ttccatcaac aaaaatccaa caaataatat catttatcct gctgtaaaaa
    17761 aaacgctatc ccggcgctgg gtaacatcgg gttcatgcta agatagagcc ttgtcccccg
    17821 caggattgat atggggtatt ttcgatttca gattaccgat gattcacgac gcttatgacg
    17881 aagaaaaaag cacataaacc tggttcagcg accatcgcgc ttaacaagcg cgcccgtcac
    17941 gaatacttta tcgaagaaga gttcgaagcg ggacttgccc tgcaaggctg ggaagttaaa
    18001 tccctgcgcg caggaaaagc caatatcagc gacagctacg tccttctgcg tgacggagag
    18061 gcatttctgt ttggcgctaa catcacgcca atggccgtgg cctccacgca tgtggtgtgc
    18121 gatcctaccc gtacccgcaa gttacttctc aaccagcgcg aactggactc attgtacggt
    18181 cgcgtcaatc gagaaggcta taccgtagtg gcgctctccc tgtactggaa aaatgcctgg
    18241 tgcaaagtga aaatcggcgt cgccaaaggt aaaaaacagc acgataaacg ttcagatatc
    18301 aaagaacgcg aatggcaggt ggataaagca cgcatcatga aaaacgccca ccgttaaacc
    18361 tgcactccaa ttattgacca gttcctcacc gcgcctccct ctccggcggc gcgaatgaac
    18421 atcttattgg ctatcacatc cgacacaaat gttgccatcc cattgcttaa tcgaataaaa
    18481 atcaggctac atgggtgcta aatctttaac gataacgcca ttgaggctgg tcatggcgct
    18541 cataaatctg gtatacttac ctttacacat tggggctgat tctggattcg acgggatttg
    18601 cgaaacccaa ggtgcatgcc gaggggcggt tggcctcgta aaaagccgca aaaaatagtc
    18661 gcaaacgacg aaaactacgc tttagcagct taataacctg cttagagccc tctctcccta
    18721 gcctccgctc ttaggacggg gatcaagaga ggtcaaaccc aaaagagatc gcgtggaagc
    18781 cctgcctggg gttgaagcgt taaaacttaa tcaggctagt ttgttagtgg cgtgtccgtc
    18841 cgcagctggc aagcgaatgt aaagactgac taagcatgta gtaccgagga tgtaggaatt
    18901 tcggacgcgg gttcaactcc cgccagctcc accaaaattc tccatcggtg attaccagag
    18961 tcatccgatg aagtcctaag agcccgcacg gcgcaagccc tgcgggcttt tttgtgctcc
    19021 gaatttgtcc cgcgaagtct gatgccaact aattaaatcc gatcctttta ggcaccttgt
    19081 taggcacttc ataaagctat attgtctttg aggtgcctaa aactatggaa acccggcaat
    19141 ggcaagacaa accaaacctc tatccgttaa ggaaatcgaa tctgctaaac ccaaggaagc
    19201 ggactacgtt ctctatgatg gcgatggcct tgagctactc attaaatcca gcgggagtaa
    19261 aatctggcag tttcgctaca ttcgccctgt caccaagaca cgagcaaaga aaagcatagg
    19321 cccttacccg tcagttaccc ttgccgatgc cagaaactac cgtgcagagt cccgctcact
    19381 cctagctaaa cagattgatc cgcaggaaca tcagcaagaa caattgcgta gttcgctgga
    19441 agccaaaacc aatactttcc agctcgtagc tgaacgttgg tggaatgtga agaaagccag
    19501 tgtgacagag gactatgcgg aagatatctg gcgctctctt gaaagagatg tctttcctgc
    19561 gattggcgac gttagcgtta cagatattaa agctcataca ctggttcagg ccgtacaacc
    19621 ggttcaggcc agaggagcac tggaaacggt tcgtcgcctg tgtcaacgta ttaatgaggt
    19681 catgatctat gcccagaaca cagggctgat tgatgctgtt cccagcgtta atatcggtaa
    19741 agccttcgaa aagcctcaga aaaagaacat gcccagcatt cgaccggatc agctatctca
    19801 actgatgcag acaatgcgaa cagccagcat tagcctttcc acacgctgcc tgttcatgtg
    19861 gcaacttctt actattaccc gccctgccga agcggctgaa gctcgctggg aagaagtaga
    19921 catagaagcg caagagtgga agattcccgc agcacgcatg aaaatgaacc gcgaccatac
    19981 tgttccattg tcagatgaag caatagctgt gctggagatg atgaaaccgc taagcggcaa
    20041 ccgagaattt atctttccta gccgtatcaa gcccaaccag cttatgaaca gtcagactgt
    20101 taacgcctca ctaaaacgcg caggttttgg tggtgtgctt gtttcgcatg gtctgagatc
    20161 aattgccagt acggctctta atgaacaagg ctttccgcct gatgccattg aggcagcatt
    20221 ggcccatgtg gataagaatg aggttcgtcg tgcttataac cgcagcgatt acctggaaca
    20281 gcgtcgcccg atgatgcaat ggtgggcaaa ttttgtgatg gcagcggatc gtggaagcat
    20341 gattgaaggt gggataaaag gaatgaagct agttgggtga ctaaatagct gcgcggcata
    20401 gtgtcccact ttcagccagc ccttgcaggt taagccacca gacagtaaac ggcgggtgtc
    20461 gcgttattag gcgcagctat ttagtagtaa tattaggatt ggtgattctg cactccagaa
    20521 gtt
//
LOCUS       DAETQL010000073        20292 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.073, whole genome shotgun sequence.
ACCESSION   DAETQL010000073 DAETQL010000000
VERSION     DAETQL010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.073"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(66..824)
                     /gene="aroD"
                     /locus_tag="K0W65_002891"
     CDS             complement(66..824)
                     /gene="aroD"
                     /locus_tag="K0W65_002891"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBI3096081.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(855..1721)
                     /gene="ydiB"
                     /locus_tag="K0W65_002892"
     CDS             complement(855..1721)
                     /gene="ydiB"
                     /locus_tag="K0W65_002892"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBI3096082.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1733..3004)
                     /locus_tag="K0W65_002893"
     CDS             complement(1733..3004)
                     /locus_tag="K0W65_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3096083.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLSEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3187..4392)
                     /locus_tag="K0W65_002894"
     CDS             complement(3187..4392)
                     /locus_tag="K0W65_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3096084.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4492..4848)
                     /locus_tag="K0W65_002895"
     CDS             complement(4492..4848)
                     /locus_tag="K0W65_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HBI3096085.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4984..5091)
                     /gene="rprA"
                     /locus_tag="K0W65_002896"
     ncRNA           complement(4984..5091)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="K0W65_002896"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5277..6389)
                     /gene="ydiK"
                     /locus_tag="K0W65_002897"
     CDS             complement(5277..6389)
                     /gene="ydiK"
                     /locus_tag="K0W65_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBI3096086.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6778..9834
                     /locus_tag="K0W65_002898"
     CDS             6778..9834
                     /locus_tag="K0W65_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBI3096087.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9831..10241
                     /gene="menI"
                     /locus_tag="K0W65_002899"
     CDS             9831..10241
                     /gene="menI"
                     /locus_tag="K0W65_002899"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBI3096088.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10341..10529
                     /locus_tag="K0W65_002900"
     CDS             10341..10529
                     /locus_tag="K0W65_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HBI3096089.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11077..11445
                     /gene="sufA"
                     /locus_tag="K0W65_002901"
     CDS             11077..11445
                     /gene="sufA"
                     /locus_tag="K0W65_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBI3096090.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11454..12941
                     /gene="sufB"
                     /locus_tag="K0W65_002902"
     CDS             11454..12941
                     /gene="sufB"
                     /locus_tag="K0W65_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBI3096091.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12951..13697
                     /gene="sufC"
                     /locus_tag="K0W65_002903"
     CDS             12951..13697
                     /gene="sufC"
                     /locus_tag="K0W65_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBI3096092.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13672..14943
                     /gene="sufD"
                     /locus_tag="K0W65_002904"
     CDS             13672..14943
                     /gene="sufD"
                     /locus_tag="K0W65_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBI3096093.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14940..16160
                     /gene="sufS"
                     /locus_tag="K0W65_002905"
     CDS             14940..16160
                     /gene="sufS"
                     /locus_tag="K0W65_002905"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBI3096094.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16173..16589
                     /gene="sufE"
                     /locus_tag="K0W65_002906"
     CDS             16173..16589
                     /gene="sufE"
                     /locus_tag="K0W65_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBI3096095.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16738..17742
                     /gene="ldtE"
                     /locus_tag="K0W65_002907"
     CDS             16738..17742
                     /gene="ldtE"
                     /locus_tag="K0W65_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBI3096096.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17805..18041)
                     /gene="lpp"
                     /locus_tag="K0W65_002908"
     CDS             complement(17805..18041)
                     /gene="lpp"
                     /locus_tag="K0W65_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HBI3096097.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18352..19764)
                     /gene="pykF"
                     /locus_tag="K0W65_002909"
     CDS             complement(18352..19764)
                     /gene="pykF"
                     /locus_tag="K0W65_002909"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HBI3096098.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5320 a         4861 c         5177 g         4934 t
ORIGIN      
        1 aaatacggtt aaaaatactt tttttatttc ggcgggggag ggtatttccg ccgaaatatt
       61 attgcttatg cctgatgtaa aatagttaat accgtgcgca aatcatttac cgagatttgc
      121 cctggcgcag aggctttttt taccgcacca aaagttgccg ccgagccaaa tacttcacca
      181 accagacgag aaattacgcc agtttttgcc atcgacatcg tgatgattgg acgatcggca
      241 tactgctcct gcatctccag ggtcgcggca agcaacgtca gcacatcgct ggtactttgc
      301 ggcatcagcg caatcttagg aatatcggcg tcgaaggact gcattttgcg cagacgggca
      361 atgatttctt cggcttccgg cgttttatgg aagtcatggt tggacatgac aactttcaca
      421 tcatgcgcgt gggcgtaggc gacggtttct ttgacctgat catcgccggt aaataactcc
      481 agatcgatca tatcaaccag gccgctgtcg atggctgcac gattgagagc aatataagcc
      541 tcggtggaaa tcgcctgctc gccgccttct ttggcactgc ggaaggtaaa cagcagcggt
      601 ttttctggca tggtttcacg gagaattttt gccgccgcca tgacagactc cacattggag
      661 aggtcggcaa agtggtccac acgccattcc agaatatcaa agtccgcttc acgataggcg
      721 agagcttcgg atttcacgcg ggcgatatct ttcgccatca gcgagacgat gattttaggt
      781 gcgcccgcac caatgacgag atcttttaca gttacggttt tcatttttta ccctttctgc
      841 acgcggtcag cctgtcaggc accgaacccc atgacctgtt taacatattc cagagggaaa
      901 tctttgccag tccataacgt gaactgttca gccccttgcc acaacaacat gccgtatcca
      961 tcaatcgttt tgcaaccagc ttgttgtgcc tgctgcaata acttcgtcat atgcgggtta
     1021 tacacgcatt cagtgaccag cagtcccgga tgtaacagac tgatatcatt aaccaatgat
     1081 tcattctcaa ggggtttcat acctactttt gtgccattgg ttaaaatgtc ggcggaagcc
     1141 agggcttcag caaaggcttg ctgatcggcg agatcggtat ccgtgacgac acaatcggtg
     1201 ttttcgttaa cccgctgcgc gaaggcgagg gctttatcga agaactcatc ccgacggtta
     1261 aagagtttaa tttcttttaa accttcaatt gccccctgcg cgccaatcgc cgttgaggca
     1321 cctccggccc ctaacagcac catcgttttg cctttgatat caaaaccgct ctctttaatg
     1381 gcgcgaatat ggcccgtgcc gtcggtgtta tagccacgca gatagccatc atcattaacg
     1441 atggtgttga tggcacccac cagtttggct gctggcgtta attcatcaac atattcacac
     1501 gccagttgtt tgttcggcat cgacacacca gttccgcgca ttttgagggc ttttaatcct
     1561 tcaattgctc cgggaaagct atcgttatcc acttcgaagg ccatataggt aaatggcaat
     1621 cccgcttttt ctaaggcttt attctgcatt tcaggcgata aactgtggcg gataggataa
     1681 gccatcaatc caatcaattc gtattttgcg gtaacatcca taattaactc ctttaacctc
     1741 tatgcttaat tgtgtttaac cgggtgctaa atttgcgttc gccaaaccgc acatcattct
     1801 caggaataat aaaaacgcgg taatagcggc taaaaacaat aattgcggta atcagggcca
     1861 gcagcgcgaa agtaaaatcg agaacaatga tatattgcag gccgatgttc gacagataac
     1921 cggtaatcag cggaataaca aagttagcca gtccacccat catcatataa atactggtga
     1981 ctttggcttt gcttttgggg aaaaactctg acatgaccga aacaccgagc tgtaaaatgc
     2041 cgccagctgc tgagaaaccg ataacaaagg cgccggcatt acacaccagt ggagaagggt
     2101 acaggtagat aatggcagct gttattgttg ccagcgcaga gttaaataca ttagcccaga
     2161 tgggccggac catttttttc agtagtgcgg caaaaataaa gacacagacc aacgagccca
     2221 tactgtaata agagatggtt tttaatgcct cagcttctga cataccagca aaagccatcg
     2281 catatttggg catccacacc acaatcacat aaaaggtcga gaatgcggct acaccgaaca
     2341 gtaccgatga aacaccttcc agccagacta aaggtttgct gttcatttgc ggtaattcat
     2401 tagctacgct ggcgtccacc aactggctgg ggaatttgct tttcaacagc atcagcgtga
     2461 tcagtacaaa cagaataccc ggaataatca gcccatagcc gtaccagata ttattgagca
     2521 acatatagct caccagcatt gggtagaaca tttgcccaaa tgacaccatc gctttaacca
     2581 gtatgaccgc cgaaccagag gctttcggaa agcattccat gagcgcgggg tagccacccg
     2641 tatccagcgc tgagttagcg atacctacgc acactgccag accgtaggcg agagttaaat
     2701 tcgggcaagc gggaatacca aagaagaata gcagatacat tattactgcc attaatatca
     2761 ccgcccgacg accaaactta tcggagatca caccgaagaa taaaatactg atcaatcgcc
     2821 ccaaaccgat accggaaatt aaataggcaa tgcccgcgtt gtcagtggaa aacttttctg
     2881 acagagatgt catattttgg gcaagcgtaa taacactaat gccgtgcagg aaatagctga
     2941 agtaaataca aagaacagcc aggataaatg gcgtgctgaa agataatttc gttaccgtac
     3001 gtattgttct ccgacggttt acataattac tatactgaag atgattttcc ctgggtacat
     3061 ggcataaagt ttatgtttcc aggaatattg catgtcatag tttgtttttg atcgcgattt
     3121 tccttagtta gcgcagaaag agttatttat ccgtaagatc ttgcatgacc tgaaaagaat
     3181 attgcattac ccacccggag cgacattttc ctttagcgag tgatgccgcg tttttttgcg
     3241 gctgcgtaag ccgataaaca gtgccagtaa aaaaccgatg gcagtatcga accacataat
     3301 atcggcaata cttctttggg acagatgagc cgtaatcaac ggaatagtaa aggtcgcaat
     3361 actgcccgta ctgtaataga tccctgtagc tttaccttta gcgtaaggga aacgttcagc
     3421 cattaacgtc aggccaattt gcacaacacc tccagcagag gtaaaaccaa tgacaaaagc
     3481 aaatattatc accacataaa atgtgggatg caggcagacg gtaaacagag caataaatga
     3541 gataaaggtg tacagcatca gtaatgttgt tgggcgaacg gtattacgaa tgagtggagc
     3601 ggtaataaat acacaaagca gcgaacccac ggtgtagata ctgagtagtt tgatcgacat
     3661 agtgtatgac atgcctgcaa caaattgtcc gtactgtgcc agccactggc taaccagata
     3721 aaacgttgcc attgagatat agccatataa ggtataactg gctaaatcga taattgaaca
     3781 gcgatgttcc gtagagctgg tggttttctt tatgacaggt aagcgacgac ccggatgggg
     3841 tgggaacgta caacggtata aaaacagagc gttaataaac ataatgcctg cagcaatcat
     3901 aaaggaccaa ccgaaccaca gttcagccca cactaacagg ctaatgatta gcggtaataa
     3961 aaattgtccg ctggaaacaa atgctttaat taaaatattg gctgtcccag gtgagcgtgg
     4021 aaaagcttcc atcaaactgg gataagtgcc tgcatcgaga aaactgtttg ccattcccgc
     4081 cagaaagcca aaaacataag cgataatgat gttattggtc tgcaggatgt caaaaaagaa
     4141 ggccatatag cagcacatcc cgagcatgat aaaagggcgg cgaccaaagc gatcggataa
     4201 taatcctgca aaaagcaaga cacttaatcg accaatgccc agcgatgaga taactatcga
     4261 gacacccgcg gcattagtct gccaaagtgt ctccagcgag gccatattca ggctcatcaa
     4321 aatgacgccc ataccatgca ccaggtaatt aaaatacaac ccaagtgcgg tagggtaata
     4381 gggatttttc ataggttcta cttagcaaag gtgctttaaa aagtgtagcg atgttgtgtc
     4441 agtatccatt cattattgtt gtaacaacga caattccttt ctgtggaaaa attaacaaag
     4501 ccgctccaga tcgtgtgcat ataattcagc ggctaccgat tgatagatct tccagtcaat
     4561 aaaattgcca tcagggtaga cttgttgaaa cgcggttaag tcggggaaaa aacgcatggt
     4621 gttgttgccg atgcggttat ttattttctc tatgatggca tgaagatgtt tttttcgttg
     4681 ctgacgcata gccaacagtt gctcgactac acaatcagga atggcgcatt tgccattttc
     4741 ccactgacgc cagctttcac tgtcaccggt ctgggcaatc caggtcgcgc attcatcaat
     4801 agtcatggca aaaatatggc ggagtgcctg gagttcataa gcgttcatcg tgtaatggct
     4861 gcatgaaata aacaataaaa tataaataac attatcagcc tgctgacggc ttgaagagag
     4921 tcacagtatc ttgtgcaaca ttattggaaa gagtgagggg cgaggtagcg aagcggaaaa
     4981 atgttaaaaa aaagcccatc gtgggagatg ggaaaagact acacacagca attcgttgtt
     5041 tcactcaggg gatttccatg cttataaatc aatatgttga tttataaccg tgagctaata
     5101 gtaggcatac ggactttttg cgtcgatcag attcgtctca ttagtttaaa aactgtaaag
     5161 aatttgcgac aggtactacc agctgaatgg cggtaagcag gaaaatatgg ggaagagcaa
     5221 gcaaatatca attagcggtg attaacaatc aggcggcaga gccgcctgat gagaaattac
     5281 ttattcggtt tctcgatttc gccaagttct tcgagaattt cttccggttg gtccgtcggt
     5341 ggcgggactt cttccaccca cgcggcaaac agacgccagg aaacggctaa cagaaccgga
     5401 ccaataaaca gaccgatcat cccgaaagca atcaaaccac caataacgcc agagaggatc
     5461 aggatcagcg gtaaatcggc acccatgcga attaacattg ggcggatgac gttatccagc
     5521 gtgccaacca caccgctcca cactaacaat accgttcccc aggtggtatc gccggtccag
     5581 tagagccaga taatcgccgg aatcagtacc ggcaacgggc caagctggac aaggcaggag
     5641 aggatcatta acaccgttag caacgttgca taaggtacgc cggatacggc gaggccgata
     5701 ccgccaagca ctgcctgtac taacgccgtc accaccacac ccagcgccac cgcgcggata
     5761 gcctgtgccg ccagcagcac ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa
     5821 tggcgaatgc cttgtgccac ctgttcaccg cgccagtaca gcagggcact gaagagaagc
     5881 atcagcgcac aatgcaccat aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg
     5941 gtggtggtgc caatataagg gcggactttc gccatgatcg ccgtaccccc catatccagc
     6001 aagttgtgcc agcctgcata cagcttcgcg ccaatcaccg gaatggtatt aagccacgcc
     6061 agatcgggta acgtcatgtc gccgctggaa atggctttaa ttagcgggcc gctgccgtcg
     6121 acgatactgt taaccagcaa agcaataggg atgataaaca ccatcactaa taacagcgtc
     6181 atcaccagaa cggcgagaga gcggcggcca aacatgatct tttgcaaacg taacaatacc
     6241 ggccaggtgg cgataaccac cgtaccggcc catgcaaagc cgagaataaa gggttgaaca
     6301 atccacagac atgccacaat catgatggct aaaaacagca ccgaaagcag aatttgtgcg
     6361 acatccctgg gctgacgaac atttaccata actacttttc accttttttg tgcgtcaaat
     6421 cgtcgacgcg acgtgaacac gcgaaactca cctgcataat gatgagcaat ttcagcggtt
     6481 tttaacaggc cgattctgcc cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa
     6541 caactcaggc aacacgcaaa cgatttactc gtcgtatttc aagttgcatg tgctgcggct
     6601 acgttcgttc accccagtca cttactattg taagctcctg gagttcactc gcttgccgcc
     6661 ttcctgcaac tcgaaatcca tagagtaaat aaacatcacc tttcagtgcg ctattgcagc
     6721 cgcctgaaag aatagtgtat cgacctacat cacagaccgc aggaaagggt caatataatg
     6781 attccacaga tttccctggc acccggcgtc gttcaactgg tgcttaattt tttgcaagag
     6841 ctggagcaac aaggttttac cggcgatgcg gcgacaagtt atgccgatcg tctgacaatg
     6901 tcgaccgaca acagtattta ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc
     6961 gcagatgtgg cgctgatcgc ccgtcttgcc gcgcaggaac gctattcatc gctgatcttt
     7021 accccccgcg gcggcggcac cggcactaac ggtcaggcgc tcaaccaggg gattattgtt
     7081 gatatgtccc gccatatgaa ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc
     7141 gttgaggccg gggtgataaa agaccaactc aatcagtacc tgaaaccgtt cggctacttt
     7201 tttgcgccgg aactttcgac cagcaaccgg gcaacgctcg gtgggatgat caataccgat
     7261 gcatccggtc agggatcgct ggtctatggc aaaacgtcag atcacgtact tggcgtacgc
     7321 gcggtgttgt tggggggcga tattctcgat acgcaacctt tacccgtcga gctggcggaa
     7381 acgctgggta aatccaatac cacaatcggg cgaatttata acacggttta tcaacgttgc
     7441 cgtcagcaac gccagttaat catcgacaac ttccccaaac ttaaccgctt tcttaccggt
     7501 tacgatctgc gtcatgtctt taacgatgag atgaccgagt tcgacctgac gcgcattctg
     7561 acgggttcag aagggacgct ggcctttatt accgaagcgc ggctggatat tacgcgcttg
     7621 cctaaagtgc gccgtctggt gaatgtcaaa tatgactctt ttgactctgc gctgcgtaac
     7681 gcgccgttta tggttgaggc gcgggcgctt tcggtagaga cggtggactc aaaagtgctg
     7741 aatctggcgc gggaagatat tgtctggcat tccgtcagcg agttgattac cgatatgcct
     7801 gacaaagaga tgctcggact gaacattgtg gaatttgctg gtgatgatga ggcgctgatt
     7861 gacgagcgag ttaatgcact ctgtgtgcgg cttgatgagc tgattgccag ccaacaggca
     7921 ggtgtgattg gctggcaggt gtgccgcgag ctggcggggg ttgaacgtat ctatgcgatg
     7981 cgcaaaaaag ccgttggtct gcttggcaat gccaaaggtg ccgctaagcc aattccgttt
     8041 gctgaggata cctgcgtacc gccggaacat ctggccgatt atattgctga atttcgcgcg
     8101 ctgctcgaca gccacggctt aagctacggt atgttcggtc acgtcgacgc aggtgtcttg
     8161 cacgtccgtc cggcactgga tatgtgcgat ccgcaacaag agattttgat gaagcaaatc
     8221 tctgatgacg tggtggcgct gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc
     8281 aaaggttttc gcgctgaata cagcccggcg tttttcggtg aggaactttt tgcagaactg
     8341 cgcaaagtga aagcggcatt tgacccgcat aaccgactca acccagggaa gatttgcccg
     8401 ccagaaggtc tcgatgcgcc gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat
     8461 cggcagatcc ccattgcggt acgccagcag tggcgcggtg cgatggagtg taacggcaac
     8521 ggtttatgct tcaactttga tgcccgtagt ccgatgtgtc cgtcgatgaa gatcacccag
     8581 aaccggattc attcaccgaa agggcgcgca acgctggtgc gtgaatggct gcgcttgttg
     8641 gcggatcgcg gcgttgaccc actcaaactg gaacaagaac tgcctgaatc cggcgtcagt
     8701 ttgcggacgt taattgcccg cacgcgcaat agctggcatg cgaataaagg cgaatatgac
     8761 ttctcacacg aagtcaaaga ggcgatgtcg ggctgtctgg cctgtaaagc ttgttcgacc
     8821 cagtgcccca tcaaaattga tgtgccggag tttcgctctc gttttctgca gctctatcac
     8881 acccgttatt tacgcccgct tcgcgaccac ctcgttgcta cggtcgagag ctacgcaccg
     8941 ctgatggcac gcgcgccgaa aacctttaac ttcttcatta accagccgct ggtgcgcaaa
     9001 ctctcggaaa aacatatcgg catggttgat ttgccactgc tgtcggtccc ctcgctacaa
     9061 caacaaatgg tggggcatcg ctcggcaaac atgacgctgg aacagcttga agctctcaat
     9121 gcagagcaga aagcgcgcac ggtgttggtg gtgcaggacc cctttaccag ctattacgat
     9181 gcgcaagtgg tggcggattt tgtccgtctg gttgaaaaat taggtttcca gcctgtgtta
     9241 ctgccatttt cgccaaatgg caaagcccag catatcaaag gctttcttaa tcgttttgcg
     9301 aagacggcga aaaagacggc ggatttcctc aaccgtatgg ctaagcttgg gatgccaatg
     9361 gtgggcgtcg atccggcgct ggtactttgt tatcgcgatg aatataaact ggcgctgggc
     9421 gaggaacgtg gcgagtttaa cgtcttactt gcgaatgaat ggctggcaag cgcacttgag
     9481 tcacatccag tggctacagt cagcggtgaa tcatggtatt tctttggtca ctgtaccgag
     9541 gttaccgctt tgccgggcgc gccagcacaa tgggccgcga tatttgcccg ttttggcgcg
     9601 aaactggaaa atgtcagcgt gggttgctgc ggcatggcag ggacttacgg acatgaagcg
     9661 aaaaaccatg aaaattcgct cgggatctat gagttatcct ggcatcaggc tatgcagcga
     9721 ctgccgcgta accgctgtct ggcgaccgga tattcctgcc gtagccaggt aaaacgggtt
     9781 gaaggcacgg gggtacgcca tcctgtgcag gctttactgg agattattaa atgatatgga
     9841 aacggaaaat caccctggaa gcactgaatg ctatgggtga aggaaacatg gtggggttcc
     9901 tggatattcg ctttgaacat attggtgatg acacccttga agcgacaatg ccagtagact
     9961 cgcggacaaa gcagcctttc gggttgctgc atggaggagc atccgtggta ctggccgaaa
    10021 gtatcggttc cgttgccggt tatttatgta ccgaaggtga gcaaaaagtg gttggtctgg
    10081 aaatcaatgc taatcacgtc cgctcggcac gagaagggcg ggtgcgcggc atatgtaaac
    10141 cgttgcatct cggttcgcgt caccaggtct ggcagattga aatcttcgat gagaaagggc
    10201 gtttgtgctg ttcgtcacga ttgacgaccg ccattttgtg acgttgtcac cagaaaagtg
    10261 tgacgcatat tgcttttggt cattatctgc acagtaaacg gtggtatact gtgcaggtct
    10321 tgtctaaaag cgaggatgct atgtctactc aacttgatcc cacccagctg gccattgaat
    10381 ttttacgccg tgaccagagc aatctttcac ctgcccaata tctcaaacgt ctgaaacaac
    10441 tagaacttga atttgctgac ctgcttactc tctcttctgc tgaattaaaa gaagaaatct
    10501 actttgcctg gcgtctgggc gtgcattgat cctgacttaa tgccgcaagt tcgccaaatc
    10561 gacctggatt aacctccgtc caggttcgat gaccttctta atactgctta tctaccgcct
    10621 gatattgttc aaaaataata ctaatcgcag tttgtgttaa aacggcgggt tagctttatg
    10681 agattattct tatcgcccct tcaagagcta agccactgag agtgccggag ataagcgccg
    10741 gatggggtag aaacccttaa gcctgtgtcg cacagactta agggttttct tatttctgaa
    10801 cgctgcactt ttttacctat ctcttaaata gtcattttca atacaacttt ttctttttca
    10861 tcacagcctt aatgctcctt agataataac cattatctaa caatgagata cctaattctt
    10921 agcgtgcctg ttaacccaca catcaggggc tatgcttatt aaaagcaggc aaaaagaatg
    10981 ttttacggta aagcccctgc gtttgctggg ttgaactgat aatcattatc actaacatgc
    11041 tgttatacgc tgaaagcgat gaagtgaggt aaatagatgg acatgcattc aggaaccttt
    11101 aacccacagg atttcgcctg gcaaggctta acgctgacac ccgcagcggc gatacacatc
    11161 cgcgagctgg tggcaaagca gccaggtatg gtcggcgtgc gcttaggtgt gaagcaaacg
    11221 ggctgcgcgg gctttggcta tgtgctcgac agtgttagcg agccggacaa agacgatctg
    11281 ctgtttgaac acgacggcgc gaagctgttt gtcccgctgc aatcgatgcc gtttattgat
    11341 ggcacggaag tcgatttcgt tcgtgaagga cttaatcaga tattcaaatt tcacaaccct
    11401 aaagcccaga atgaatgtgg ctgtggcgaa agctttaggg tataggcggt actatgtctc
    11461 gtaatactga agcaactgac gatgtcaaaa cctggaccgg cggcccgctg aactataaag
    11521 aaggattctt cacccagtta gccaccgatg agctggcaaa ggggataaac gaagaggtgg
    11581 tgcgcgcaat ttcggcgaag cgtaatgagc cggagtggat gctggagttt cgtctaaacg
    11641 cctatcgcgc atggctggag atggaagaac cgcactggtt gaaagcgcac tacgacaagc
    11701 tgaattatca ggattacagc tactactcag caccatcgtg cggtaattgt gacgacactt
    11761 gcgcgtctga acctggcgcg gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg
    11821 tggaggcggc gtttgagcag ttgggcgttc ccgtgcggga aggcaaagag gtggcggtgg
    11881 atgccatttt cgactctgtt tcggttgcca ccacttatcg cgaaaaactg gcggagcagg
    11941 gaattatttt ctgttccttt ggtgaggcga tccacgatca cccggaactg gtgcgtaaat
    12001 atctcggcac cgtggtgccg gggaatgaca acttctttgc agcacttaat gcggcggtag
    12061 cctctgacgg tacgtttatt tatgtgccta aaggcgtgcg ctgcccgatg gaactttcca
    12121 cctattttcg cattaacgcg gaaaaaaccg ggcagtttga gcgcacaatt ctggtggccg
    12181 acgaagacag ctacgtcagc tacattgaag gctgttccgc tccggtgcgt gacagctatc
    12241 agttgcacgc ggcagtggtg gaagtcatca tccataaaaa cgccgaggtg aaatattcca
    12301 cggtacaaaa ctggttccct ggcgataaca acaccggcgg tattctcaac ttcgtcacca
    12361 agcgtgcttt gtgcgaaggc gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt
    12421 cagcgattac gtggaaatat cccagctgca ttttgcgcgg cgataactcc attggtgagt
    12481 tttactcagt ggcgctgacc agcggtcatc agcaagcgga taccggcacc aagatgatcc
    12541 acatcggtaa aaacaccaaa tcgaccatta tctcgaaagg gatctctgcc ggacatagtc
    12601 agaacagtta tcgcggctta gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca
    12661 ctcagtgcga ctcaatgctg attggcgcta attgtggggc gcataccttc ccgtatgtcg
    12721 aatgtcgcaa taacagcgca caactggagc acgaggcaac gacatcacgt attggtgaag
    12781 atcaactgtt ttactgcctg caacgcggga tcagcgaaga agacgccatc tcgatgattg
    12841 ttaacggttt ctgcaaagac gtgttctcag aactgccgct ggaatttgcc gttgaagcac
    12901 aaaaactcct cgccatcagt cttgaacaca gcgtcggata aggaataaac atgttaagta
    12961 ttaaagattt acacgtcagc gtggaagata aagctatcct gcgtggatta agcctcgacg
    13021 ttcgtcccgg cgaagttcac gccattatgg ggccaaacgg ttcgggcaaa agtaccttat
    13081 cggcaacgct tgccgggcgc gaagattatg aagtgacggg cggcacggtt gagttcaaag
    13141 gcaaagattt gcttgcactg tcgccggaag atcgtgcagg cgaaggcatc tttatggcct
    13201 tccagtatcc ggtggagatt ccaggcgtca gtaaccagtt tttcctgcaa acggcactta
    13261 atgcagtgcg cagctatcgc ggccaggaaa cgctcgaccg ctttgatttt caggatttga
    13321 tggaagagaa aatcgctctc ctgaagatgc cggaagattt attaacccgt tcggtaaacg
    13381 ttggtttttc cggcggcgag aaaaagcgca acgatatttt gcaaatggcg gtgctggaac
    13441 cggagttatg cattcttgat gagtcggact ccgggctgga tattgacgca ttaaaagtgg
    13501 tcgccgatgg cgtgaactcg ctgcgtgatg gcaagcgctc attcatcatt gttacgcact
    13561 accaacgcat tctcgactac atcaagcctg attacgttca tgtgctatat cagggacgaa
    13621 ttgtgaaatc cggcgatttc acgttggtca aacaactgga ggagcagggt tatggctggc
    13681 ttaccgaaca gcagtaatgc gctgcaacag tggcatcact tgtttgaagc tgaagggaca
    13741 aaacgctcac cgcaagcaca gcagcattta caacaattgc tgcgtaccgg actgccgaca
    13801 cgtaaacatg aaaactggaa atatacgccg ctggaagggc tgaccaatag tcagtttgtc
    13861 agcattgcgg gagatatatc cccacagcag cgtgatgcct tagcgttaac gttagacgcc
    13921 gtgcggctgg tgtttgtcga tggacgttac gtgcccgcgc tgagcgatgc aattgaaggc
    13981 agcggatatg aagtgagcat taacgacgac cgtcaggggt tacccgacgc tattcaggcg
    14041 gaagtgtttc tgcatttgac ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag
    14101 cgcggtcaac ggccggcaaa gccattgctg ttaatgcata tcacccaggg cgtggcaggt
    14161 gaagaggtga acactgccca ttaccggcat catctggatc tggcggaagg tgccgaagca
    14221 acggtgatcg aacattttgt cagccttaat gatgctcgtc attttaccgg ggcacggttc
    14281 actatcaatg tcgcagcgaa cgcccacttg cagcatatca agctggcgtt tgaaaacccg
    14341 gtcagtcacc actttgccca taacgatttg ttgctggctg acgatgccac cgcatttagc
    14401 catagtttcc tgctgggtgg cgcagtgtta cgacacaaca ccagtacgca actcaatggc
    14461 gaaaacagca cgctgcggat caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat
    14521 acccgtacct ggctggaaca caataaaggt ttttgtaaca gccgacagtt gcacaaaact
    14581 atcgtcagcg acaaaggccg cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc
    14641 atcaaaacgg atggtcagat gaccaacaat aatctgctga tgggcaaact ggcggaagtg
    14701 gatacaaaac cgcagctgga aatctatgca gatgatgtga aatgcagcca cggcgcgacg
    14761 gtggggcgta ttgatgatga acagatgttc tatctgcgct cgcgcgggat caatcagcag
    14821 gatgcccagc agatgatcat ttacgccttt gctgccgaac tgacggaagc actgcgtgat
    14881 gaggggctta aacagcaggt gctggcccga atcggtcaac ggctgccagg aggtgcaaga
    14941 tgactttttc cgtcgacaaa gtgcgggccg actttccggt gctttcgcgt gaggtaaacg
    15001 gtttgccgct ggcttatctc gacagcgccg ccagtgcaca gaaaccgagc caggtgattg
    15061 acgccgaggc cgagttttat cgtcatggct acgcggcggt gcatcgcggt attcatacct
    15121 taagcgccca ggcgaccgag aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta
    15181 atgcccgttc ggcggaagag ctggtgtttg tccgcggcac gacggaaggg atcaatctgg
    15241 tcgccaatag ctggggcaac agcaatgtgc gggcgggcga taacatcatc atcagtcaga
    15301 tggagcacca cgctaacatt gtcccctggc agatgctttg cgcacgcgtt ggcgcagagc
    15361 tgcgtgtgat ccctctcaac cccgacggta cgctgcaact ggagacgctg cctacgctgt
    15421 ttgatgagaa aactcgcctg ctggcaatta ctcatgtctc caacgtgctt ggcacagaaa
    15481 atccactggc ggaaatgatc acgcttgcgc accagcatgg cgcaaaagtg ctggtggatg
    15541 gcgctcaggc ggtgatgcat catccggtgg atgttcaggc gctggattgc gacttttacg
    15601 tgttctccgg gcataaactg tatggcccca ccggaattgg cattctttat gtgaaagaag
    15661 ccttgttgca ggagatgccg ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc
    15721 tgagtgaagg cactacctgg accaaagcac catggcggtt tgaagccggt acacccaata
    15781 ccgggggcat cattggtctt ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata
    15841 gcatagccga gtatgaacag aatctgatgc attatgcgct atcacagctg gaatctgtac
    15901 cggatctcac tctctatggc caacaaaaca ggcttggcgt tattgctttt aatctcggta
    15961 aacaccacgc ctatgatgtt ggcagttttc tcgataatta cggcattgct gtgcgtaccg
    16021 gacatcactg cgctatgcca ttaatggcct attacaacgt ccctgcgatg tgtcgggcgt
    16081 cgctggccat gtataacacc catgaagaag tggagcgtct ggtgaccggc ctgcaacgta
    16141 ttcaccgtct gctgggataa cagggaggca ctatggcttt attgccggat aaagaaaaat
    16201 tgctgcgtaa ttttttacgc tgcgccaact gggaagagaa atatctctac attattgagc
    16261 tgggccagcg tctgccagaa ttacgcgccg aagacggaag cccacaaaat agcattcagg
    16321 gctgccagag tcaggtgtgg attgtcatgc gccagaatgc gcagggaatt attgaattgc
    16381 agggcgacag cgatgcggcg attgtgaaag ggcttattgc ggtcgtcttt attctctacg
    16441 atcagatgac gccgcaggat attgtcaatt tcgatgtgcg tccgtggttt gaaaaaatgg
    16501 cgctcaccca acatctcacc ccatctcgtt cacaaggtct ggaagcgatg attcgcgcaa
    16561 ttcgcgccaa agccgctgca cttagctaaa ctaaagggac agctttcatc cggttggctc
    16621 acattgccgg atggtcaatg tttacactga ccggcacagg agtgttttgg tttcaggtga
    16681 acataagcac catccgacgt ttcagcgtga gtctccggca aatacaggag gtttacaatg
    16741 aaacgcgcgt ctttgcttac actcacgctt atcggcgctt ttagcgccat ccaggctgcc
    16801 tgggcggttg attatccgct accaccaacc ggaagccgac tggttgggca aaatcaaacg
    16861 tatacggtgc aagaagggga taaaaacctt caggctattg cccgacgttt tgatactgca
    16921 gcaatgttga tccttgaagc caataacact atcgccccgg tgccaaaacc tggtacgacg
    16981 ataactattc cttcacaact gttattacct gatgcaccgc gtcaggggat tatcgttaac
    17041 cttgcagagc tgcgccttta ttattatccg ccgggagaaa atattgtgca ggtttatcca
    17101 ataggtattg gattgcaggg gctggaaacg ccggtgatgg aaacgcgtgt tgggcagaaa
    17161 atccctaacc caacctggac gcctacggca ggcattcgtc agcgttcgct ggagcgtggc
    17221 attaaattac cgccagtcgt tcctgccgga ccaaataacc cgctaggacg ttacgcactg
    17281 cgcctcgcgc atggtaatgg cgaatacctc attcatggca ccagtgcgcc ggacagcgtt
    17341 ggtttgcgcg tcagttcagg gtgtattcgc atgaatgcac cggatattaa agccttgttc
    17401 tccagcgtgc ggacgggaac gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg
    17461 gaacctaacg ggatgcgtta tgttgaagta catcgaccac tatcagcaga agaacagcaa
    17521 aacgttcaga caatgccata cacactgcca gcaggcttta cgcaatttaa agacaataag
    17581 gctgtagatc agaagttagt cgataaagcg ttgtatcgtc gggcagggta tccggtttcg
    17641 gtgagcagtg gagcaactcc cgcagccagc aatgcgcctt cagtagagtc agcgcagaat
    17701 ggtgaaccag agcaagggaa tatgttacgc gcgacgcagt agtggtaaat agcagacaaa
    17761 aaaatggcgc acaatgtgcg ccatttttca cttcacaggt actattactt gcggtattta
    17821 gtagccatgt tgtccagacg ctggttagca cgagctgcgt catctttagc agcctgaacg
    17881 tcggaacgca ttgcgttcac gtcgttgctc agctggtcaa ctttagcgtt cagagtctga
    17941 acgtcagaag acagctgatc gattttagcg ttgctggagc aacctgccag cagagtagaa
    18001 cccaggatta ccgcgcccag taccagttta gtagctttca ttattaatac cctctagatt
    18061 gagttaatct ccatgtagcg ttacaagtat tacacaaagt tttttatgtt gagaatattt
    18121 ttttgatggg aaggcactta tttttgatcg ttcgctcaaa gaagcatcga acgctgggtt
    18181 ttagataaaa aaattgagag aaaacaggat gcttccatcg gattcatctt aaataaagtg
    18241 agattatata gtgaaatccg atttgatttt ttattgcttc tggttatcga ttaaataaaa
    18301 aaagcgccca tcagggcgct tcgatataca aattaattca caaaagcaat attacaggac
    18361 gtgaacagat gcggtgttag tagtgccgct cggtaccagt gcaccagaaa ccataactac
    18421 gacgtcacct ttgtgtgcca gaccgctctg cagagccagt tctttaccca gacggtagaa
    18481 atcatcagta gaagtgatct ctttaaccag ctgcggcaca acgcctttgc tcagtaccaa
    18541 ctgatgagcc gttttttcgt tggtggtcag tgccaggatg gtggcatccg ggaagtattt
    18601 acgtactgcg cgagcagatt taccgccctg ggtagcaacc acgatcagcg gagcatccag
    18661 tttttcagca gtttcaacgg caccacggca taccgcttcg gtgatgcgca gtttacggtt
    18721 gtcattgttg aactcgagac ggctgttcat cacgcggtcg gtacgttcgc agatggtcgc
    18781 catgatagaa accgcttcca gcgggtattt accttttgcg gattcaccag acagcatcac
    18841 tgcgtcagta ccgtcgagga tggcgtttgc aacgtcaccg gcttctgcgc gagtcgggcg
    18901 tgggtttttg atcatggaat ccagcatctg ggtcgcagtg ataacgactt tacgtgcacg
    18961 gatacatttt tcgatcatca tcttctgggc gaagataact tcttctaccg ggatttctac
    19021 acccaggtcg ccacgcgcaa ccatgatgcc gtcagaggct tcgaggattt cgtcgaagtt
    19081 gttgaggcct tcctggtttt cgattttgga gatgatgtgg atgttttcgc cgccgtgcgc
    19141 tttcaggtgc tcacggattt cgataacgtc agaacgctta cgaataaagg aagcagcaac
    19201 aaagtctacg cctttttcgc aaccaaagat caggtcctgt ttgtcttttt cagccagtgc
    19261 tggcagagca atggaaacgc caggcaggtt cacacctttg ttttcgccca ggtcaccgtt
    19321 gttcagcact ttacagataa ctttgttacc ttcaatggcg gtaacttcca taccgatcag
    19381 accatcgtca accagtacgg tgttgccaac agacaggtca gtagtgaaac cttcatacgt
    19441 taccgcaacc atttcgctgt tgccgataac agatttatca gtggtgaaag taaaggtctg
    19501 accagctttc agagaaacat cgttaccgcc ttccagtttc atggtgcgga tttccggacc
    19561 tttggtatca agcaggatag cggcggtttt accagttttg ctcatcacgt tgcgcagatt
    19621 ctgaatgcgc tgaccgtgtt ctgcatagtc accatgagag aagttcagac gcataacgtt
    19681 catgccagcg tccagcattt tagctaacat ctcttcagat tcggttttcg gtccgatggt
    19741 gcaaacaatt ttggtctttt tcatgacagt cttagtcttt aagttgagaa ggatgggaga
    19801 aacttgcttt ctgggcgcac tgccggaaag cgaatccggt attacgaaag tggtggtgcc
    19861 tcgctctaag gataggtgac atcgaaaaag cgtgcagagg aatgtgtgct tgtgtctcag
    19921 cccaacgtga gtgtctgttt ttgagtcgtt gagttctaca gtccaaagtg ctgaaaccat
    19981 tcaagagtca attggcgcgc attatacgct gaaattaatc caaaaggaaa acaaaaacag
    20041 cgtttcaaaa aatatacaac ttgttagaca ataaattgtt atcaaagatt taacgttcca
    20101 gggaaaaggt tacgcaaaaa cttcgctgtt tgcgcaacgt ctggtgccga agttgtcagt
    20161 gtgaggataa gtgaaagttc atccggtgta taaatatatc atcgctcaat tgctacagtt
    20221 aaaataattt atcgcgttat ttgtctgatt ttgtttgatt tttgtttaat tggttagatt
    20281 gatatatgct ga
//
LOCUS       DAETQL010000074        19870 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.074, whole genome shotgun sequence.
ACCESSION   DAETQL010000074 DAETQL010000000
VERSION     DAETQL010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19870
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.074"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(238..2079)
                     /gene="rpoD"
                     /locus_tag="K0W65_002910"
     CDS             complement(238..2079)
                     /gene="rpoD"
                     /locus_tag="K0W65_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBI3096099.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(2274..4019)
                     /gene="dnaG"
                     /locus_tag="K0W65_002911"
     CDS             complement(2274..4019)
                     /gene="dnaG"
                     /locus_tag="K0W65_002911"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBI3096100.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            complement(4130..4345)
                     /gene="rpsU"
                     /locus_tag="K0W65_002912"
     CDS             complement(4130..4345)
                     /gene="rpsU"
                     /locus_tag="K0W65_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBI3096101.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4583..5596
                     /gene="tsaD"
                     /locus_tag="K0W65_002913"
     CDS             4583..5596
                     /gene="tsaD"
                     /locus_tag="K0W65_002913"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBI3096102.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            complement(5639..7102)
                     /gene="ttdT"
                     /locus_tag="K0W65_002914"
     CDS             complement(5639..7102)
                     /gene="ttdT"
                     /locus_tag="K0W65_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HBI3096103.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(7150..7755)
                     /gene="ttdB"
                     /locus_tag="K0W65_002915"
     CDS             complement(7150..7755)
                     /gene="ttdB"
                     /locus_tag="K0W65_002915"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBI3096104.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            complement(7752..8595)
                     /gene="ttdA"
                     /locus_tag="K0W65_002916"
                     /pseudo
     CDS             complement(7752..8595)
                     /gene="ttdA"
                     /locus_tag="K0W65_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            8802..9733
                     /gene="ttdR"
                     /locus_tag="K0W65_002917"
                     /gene_synonym="dan"
                     /pseudo
     CDS             8802..9733
                     /gene="ttdR"
                     /locus_tag="K0W65_002917"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            complement(9746..10363)
                     /gene="plsY"
                     /locus_tag="K0W65_002918"
     CDS             complement(9746..10363)
                     /gene="plsY"
                     /locus_tag="K0W65_002918"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBI3096105.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            10468..10836
                     /gene="folB"
                     /locus_tag="K0W65_002919"
     CDS             10468..10836
                     /gene="folB"
                     /locus_tag="K0W65_002919"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBI3096106.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            10927..11748
                     /gene="bacA"
                     /locus_tag="K0W65_002920"
     CDS             10927..11748
                     /gene="bacA"
                     /locus_tag="K0W65_002920"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBI3096107.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11911..13149)
                     /gene="cca"
                     /locus_tag="K0W65_002921"
     CDS             complement(11911..13149)
                     /gene="cca"
                     /locus_tag="K0W65_002921"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBI3096108.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(13213..13833)
                     /locus_tag="K0W65_002922"
     CDS             complement(13213..13833)
                     /locus_tag="K0W65_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBI3096109.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            14075..15376
                     /gene="ygiF"
                     /locus_tag="K0W65_002923"
     CDS             14075..15376
                     /gene="ygiF"
                     /locus_tag="K0W65_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBI3096110.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            15399..18239
                     /gene="glnE"
                     /locus_tag="K0W65_002924"
     CDS             15399..18239
                     /gene="glnE"
                     /locus_tag="K0W65_002924"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBI3096111.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            18287..19720
                     /gene="hldE"
                     /locus_tag="K0W65_002925"
     CDS             18287..19720
                     /gene="hldE"
                     /locus_tag="K0W65_002925"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBI3096112.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
BASE COUNT         4532 a         5074 c         5547 g         4717 t
ORIGIN      
        1 cgaggtaatc ccccagagaa gtcagtgggt tacgcagaga atcgtaacgg gataccagat
       61 caagagcctg tccaatgtgc ataaaaaaat ccggaaacaa gtgagcgttt ccggattctt
      121 acacagccac tggatcggtc aactgatcct taactgatcg gcattacacc gaaaaggtgg
      181 cgtttgtgca gagagactag tgccgggtgc ggcgtaacgc ctgatccggc ctaccgatta
      241 atcgtccagg aagctacgca acacttcaga acggctcggg tgacgcagtt tgcgcagcgc
      301 cttcgcttcg atctgacgga tacgttcgcg ggtaacgtcg aactgtttac ccacttcttc
      361 cagcgtgtgg tcggtgttca tatcgatacc gaaacgcata cgcagaactt ttgcttcacg
      421 cgcggtcagg ccagccagca cgtcgtgcgt tgccgcacgc aggctttcgg tggtcgcaga
      481 atccagcggc agctcgaggg tggtatcctc gatgaaatcc cccagatgcg aatcttcatc
      541 atcaccgatc ggcgtttcca tggagattgg ctctttggcg atcttcagca ctttgcggat
      601 cttgtcttcc ggcatcagca tacgttcagc cagttcttcc ggcgtcggtt cacggcccat
      661 ctcttgcagc atctggcgag aaatacggtt gagtttgttg atagtctcaa tcatatgcac
      721 cggaatacgg atggtgcgcg cctgatccgc gatagaacgg gtgatcgcct gacggatcca
      781 ccaggttgcg taggtggaga acttgtaacc acggcggtat tcgaatttat caaccgcttt
      841 catcaggccg atgttacctt cctgaatcag gtcaaggaac tgcaagccac ggttggtgta
      901 tttcttggcg atagaaataa ccagacgtaa gttcgcttca accatctctt tcttcgcacg
      961 gcgggctttc gcttcaccga tggacatacg acggttgata tctttaacct gctcgatggt
     1021 caggccggtt tcttcttcaa tctgctgcag tttttgcagg gcgcggtgca cttcttcaga
     1081 gacatcgtgc agtttttccg accacggctt gttcatcgca attgccgcgt tgaaccaggt
     1141 atcgctggtt tcgttgccgg taaacagggt aatgaagttt ttcttcggca ttttgcactg
     1201 ctcaacgcag agcttcatga tcagacgttc ttgcgtacga acgcggtcca tcatgacgcg
     1261 catgctgttg accaggtagt caaactgctt cggcaccagg cggaactgtt tgaatacttc
     1321 agacagtttc aggatctctt cctgagcggc agcgtgactg cgacctttcg ctttgatggt
     1381 gtcacgcgtt acaacgtact gagcgcgtag ttccgcaaat ttttcgcgag ccagttccgg
     1441 gtcgatgctg ttgtcatcat cggcgctgtc gtcatcgcca tcttcttcgt cttcatcttc
     1501 gtcatcgtcc agatcttcct gggaaagctc agaaccgacg tgagtggcgg taggtgccag
     1561 atcttcttct gcgttcgggt caacaaagcc ggtgatcaga tcggacagac gcgcttcttc
     1621 tgcttcaaca cgatcgtact gttccagcag ataggtgatc gcttccggat attcagcaac
     1681 ggagcattga acctggttga tcccgtcttc aatacgctta gcgatgtcaa tttcgccttc
     1741 gcgggtcaac agttcaacgg tgcccatttc acgcatgtac atgcgtaccg ggtcagtcgt
     1801 gcgcccgatt tcagattcca cgctggaaag cacctgcgcg gcggcttcgg cagcatcttc
     1861 gtccgcggtg ttttcagcca gcatcagatc atcggcatcc ggtgcttctt ccatcacctg
     1921 aatgcccatg tcgttgatca tttggatgat gtcttcgatc tgatctgaat cgacgatatc
     1981 ttccggcaga tggtcattga cctcggcata ggtcagatag ccttgctcct taccacgggt
     2041 gacaagaagt ttcagctgtg actgcgggtt ttgctccata agacggtatc cacacttatt
     2101 tcatgaggtt ggtgttgggc gattgacccg atgtccttca gccgttaacg ctgaaatcca
     2161 acggttgttt accgccaacg ataattacga gggcgtactt atatatttgc cgctgcctct
     2221 cagtgcggct gtcgggggct tcccgatcgc tcttcggcac ttaagccgtt aaatcacttt
     2281 ttcgccagct cctggtttaa tgtccagagc tccaggcgtt cttcgttgct taaaccatgc
     2341 gtgcgctcac gagcgattaa ctcttcctgg cgcagttcaa gcagcgaatc aaacatatgg
     2401 ttgagtgagt cggtgaaggt ttgctcagca atattcttat ctgctatatc gtcccacatc
     2461 gacagttttt caagggtggc agcattattt gtaccacgat agtgctctaa aagttgcccg
     2521 gtggtcagac ctggctggga gagacaagtg ttgaccagtt ctctgaataa gccaagtcca
     2581 gggagcttat tttcatccag attctcaagc ggcgggacca acgtcgctaa ttctggattt
     2641 tgcaccagca accctataag tatacgcatg gtcgtgcgtt ttagctgcgg aacagggcga
     2701 gaaacgccgc tctctgccgc ttttggcatt aatcgttcaa gctggctgtc atcaagtatg
     2761 cctaatttgt tgcctaattc ctgacgaaga tatattcgca gcgtttcgcc cggcacttgc
     2821 gatatcaatg gcagtgccag cgtactcaaa cgtgcgcgcc cgtcaggggt actcagatca
     2881 acttgcggca tcagactgtt aaacagaaat gcggagagtg gcatcgcctg ctccatccgc
     2941 gcttcaaacg cttctttacc ttcttttcgt accagcgtgt cagggtcttc gccatccggc
     3001 aaaaacataa agcgtagctg acggccgtct gtcatgtaag gcagcgccgt ttccagcgct
     3061 cgccatgcgg catcgcggcc tgcacggtcg ccgtcataac agcaaatgac attgttggtc
     3121 gcgcggaaca acagttgtat gtgatcggcg gtggttgacg tacctaacga cgcaacggcg
     3181 taattaatgc cgtattgcgc cagcgccacc acgtccatat agccttcgac cacaagcaga
     3241 cgattgggtt cagcgttatc ctgctgcgct tcataaagac cgtaaagctg gcggccttta
     3301 tggaaaatgt ctgtttccgg cgagttcagg tatttggggg tatcgttgcc cagcacgcgc
     3361 ccgccaaaac caatcacccg accgcgttta tcgcgaatgg ggaacatcac ccgttcgcgg
     3421 aaacgatcgt aactgcgtcc ctgatcgtta gtgaccaaca tccccgcatc aatcaatgac
     3481 tggcgatttt ctggattgcc gccaaaccgc ttcaggacgt tgtcccagcc ggggggcgca
     3541 aaaccaatcg caaagcgagc gataacctcg tggcttaatc cgcgtttttc cagatactgg
     3601 cgcgcagacg tggcaacagg ttgttgtaaa gattgttggt aaaacgtatt cagaccgtcc
     3661 atcaactgat aaagcgtttg cctctgatgg cgctcgatct ggctggggcc gctgcctgct
     3721 tcaaatggca cttcaagatt gtgcattgct gccagctctt cgaccgtttc gacgaactcg
     3781 agcttgtcgt agttcatcag gaagtcgatc gcgttgccgt gcgcgccaca tccaaagcag
     3841 tggtaaaact gtttctcacc gttaacggtg aatgacgggg ttttctcgtt gtggaatgga
     3901 caacacgcgt ggaaattctt gccctgcttt ttcagcttca cacgggcatc gatcagatcg
     3961 acgatgtcag tgcgtgccag cagatcatta atgaatacgc gtgggattcg tccagccata
     4021 ggccccgatt tttagcaatt cataaacgaa aataagccgc gcattccttt cggaagcacg
     4081 gccttacaac tacaactcgg tctgatcgga gagcaacgct ctcggggaat tagtacagac
     4141 gagtgcggcg tgcgttttcg cgagccagtt tcttcgcgtg acgtttcact gcagaagctt
     4201 tagcgcgctt acgttcggta gtcggttttt catagaactc acgacgacga acttccgcca
     4261 gaacacctgc tttttcgcag gaacgcttga agcgacgcag agctacgtcg aacggctcgt
     4321 tttcacgtac tttaattacc ggcatgtgcc tctcaccttt gattaattcg gtttgccgct
     4381 ggcatcaacg ccagcttatt tcaaaatggt gcggaatttt actgcaattg ctgctgcttt
     4441 gtaaagcacc gcggcctttt ttgaaaggga cttttataag ggtgaggagt atacacgagg
     4501 ctttctccag gggcgaacaa agttttacat caacccgcat tggtcctaca ctgcgcggta
     4561 ataaagcgag gtaaaacaag tcatgcgtgt actgggtatt gaaacttcct gcgatgaaac
     4621 cggcatcgcc atttacgacg atgaaaaagg tttgttagcc aaccaattgt atagtcaggt
     4681 gaaattgcac gctgactacg gcggcgtcgt gcctgaactg gcctcccgcg atcatgtgcg
     4741 taaaaccgta ccgttgatcc aggcggcgct aaaggagtct ggtttaacgg caaaagacat
     4801 tgatgctgtg gcctataccg caggccctgg attagtcggc gcactgctgg ttggcgcaac
     4861 cgtggggcgt tctctggcgt ttgcctggaa cgttccggca atcccggtac accatatgga
     4921 agggcatctg ttagcgccga tgctggaaga taacccgccg gaatttccgt ttgtcgcgct
     4981 gctggtgtcc ggcggtcata cgcagttaat cagcgtgact ggcattggtc agtacgagct
     5041 gctcggcgag tctatcgatg atgccgccgg tgaagcgttt gataaaaccg cgaagctgct
     5101 ggggctggat tatcctggcg gaccgttact gtcgaaaatg gcggctcagg gtactgccgg
     5161 gcgctttgtt ttcccgcgtc cgatgaccga ccgtccgggg ctggatttca gcttttctgg
     5221 tctgaaaacc tttgcggcga acacgattcg tgacaacggc accgacgacc agacgcgtgc
     5281 tgatatcgcc cgcgcctttg aagatgcggt ggtcgatacg ttgatgatta agtgtaagcg
     5341 agcgttggat cagacgggct ttaagcgact ggtcatggcg ggcggcgtga gtgctaaccg
     5401 cacgttacgg gcgaagctgg ctgaaatgat gaaaaaacgc cgcggcgaag tgttctacgc
     5461 gcgtccggaa ttttgtactg ataacggcgc gatgatcgcc tatgccggaa tggtgcggtt
     5521 taaagcaggc gcgacggcgg atctcggcgt tagcgtgcgt ccgcgctggc cgctggcgga
     5581 gttaccggct gcgtaaaact attgggtgcc ggagagcaat ttccggcacc gtcctcactt
     5641 aaagcaacac cacgggcatc cacagtaagc cggtaatcac cagcaacacg aggaatatca
     5701 gcccaaaaat cgcccccagt cgccagtaat ccgccgttgg cagataacca ctaccgtagt
     5761 aaatcggact gggtccggtg gcgtatggtg taagaatgct ccccagacca attgccgcgc
     5821 caaccatcag gcagaacacc ggcagcggga tttccggcat cgccagcgcg gcggcgatca
     5881 tcatcggtgc gagggcgcag gtatacgccg tggcgctggc gaaaaagtag cgcagtagat
     5941 aaaacaccac aatcaacgtc accatcacca tcgtcggcga ataaccactt aagctgcctg
     6001 ctaacagttt gccaaaccag ctaataaaac cggtattgtt gagtccggtc gcgagggtga
     6061 taagcgaggc cagccagaag aaaacgttcc atgccgcttt attactgaca atgtcgtccc
     6121 aactgataat gcgcagaagc agcatcagcg ccaccacgct gtaaccgacc atcgcggcat
     6181 cgatataatc accgccgaaa atccacagca ccagcgcgcc taccatcagc cccagcatcc
     6241 gtttttcacg cgaacagagc gggcccattg cctgcagttc cgtctctgcc cagcgcggca
     6301 cctgatcacc agacttcagt accggcgggt acagcacgta agccagccag ggaaccagca
     6361 gaaccagtaa aatgctgagc ggcaacatcc cgaggaacca gtcgccccaa ctcagcgtgg
     6421 cgtgagatgc gcttttcatc agtccaatta acagcaagtt aggtgccatc gccgtcagga
     6481 aaatggcgct ggtcacgcag tcggcaacaa tccccatcca catgatgtac gagccaatgc
     6541 tgcgcgaact gctgtcgttt ggttgtgatt gatagagcgg tggcaggtta cggatgatgg
     6601 gatagataat acccgcacca cgcgccgagt tggacggtgt tacaggtgcc aggatcagct
     6661 cggagaacat caccgcatag ccgagaaaca gcgtgcgatg ccccatcttt ttcaccagaa
     6721 tcagcgcaat gcggcgtcca agcccggttt tttcatagcc tgtgccaaac ataaaggcgg
     6781 cgaaaatgag ccagataacc gaattagaaa aaccagaaac tgcccaggag agggatttgg
     6841 cggtgaactt aaagcctggc tgggcgagct gttcggggct gaacagcagc cagggagaga
     6901 gtatggcgat gatggagata cccaccatcg ccaccacggc acccggcacg ggttcgagga
     6961 tcagtccaac gatcacgcca gtaaaaacgg caaagtagag ccaggtatgg ttctccagcc
     7021 ctgcgggaac cggaagtaga gcaataatgg cgatgaccgc cagcggcgca agatatcgcc
     7081 accattcagt ggaaggtttc atcgtgttat gcctgcgaaa aaggagaggg gtgagcctct
     7141 ccgggagggt tatttgatgt aatggacgtg ctcgcagatc tcttccacga tgggatcgcg
     7201 gcgttcggcg aacagctttt tgttttcggc tatcaggttg ttgccgtggg tatcaataga
     7261 gacaatcagc gggccgaact ctttgacccg gcagacccac agtgactccg gcatcccgag
     7321 ttctgtccag tgcacttctt caatctcttc tacctgggtt gccgccagca ccgcgcagcc
     7381 tgctgggaaa atcacatgta acgccttgaa tttctggcag ccttcttcgg tcagcggccc
     7441 cataccacct ttgccaacca ccagttttac gccggtctgc tcaataaatt cacgttcaaa
     7501 actttccata cgcatactgg ttgtcgggcc gacggagacc atctcccatt tgtcgccgtt
     7561 tttgcgcacg atggggccag cgtggaaaat cgctttgccg ttgagatcgt aagggatcgg
     7621 acgtttcagt tcgatcaaac ggcggtgaca aacgtcgcgg caggtcacca gcgtaccggt
     7681 caggtagatc acatcgccga cgcgaatatc ttgcagatct tcagctttga tcggggttgt
     7741 caggatcttt ttcataacgc gctccgggtg tgagacagat tttcaaaggt gagatcggca
     7801 tgaaccagca gcgtgccgcg acgatgcgcc catcactgaa ctgttgccgg tcagcccttg
     7861 tggaccaatc cccagacggt tgagtccttc ttccaggcgt agctccagtt ctgccgcttt
     7921 tggattggga tggcggctgc caatcgggcg caaaatggct ttacgcgaga gtacggcggc
     7981 ggtttccacc gaggtggcga tgcccacgcc caccagtacc ggtggacagg cgtttacggc
     8041 gagggtggag atattttcga agacgaattt caccacgcct tcgtagcctt ctgacggcat
     8101 taacactttc gagcggccag gtagcgtgca gccgccgcct gccatgtaaa cttcgatttc
     8161 cgcatcgtca ttgtcgggga tgatgtccca ggtgacccac ggtacgccgc taccggtatt
     8221 tttgccggtg tttacttcgt caaaaatttc taccgcattg tgacgtagtg gcgctttgac
     8281 ggttgcctct tccacggctt gtttgagtat gctttgcagc tcgccaagca gtgggaagcg
     8341 ggaaccgact ttaacgaaga acataatctc cccggtgtcc tgacaggcag gacgattcag
     8401 gtcaatcgct ttttgcatgt tgtcgaacat cgtatggtag ataattttcc ccatcgacga
     8461 cgtttcggca tcctttagct gttttagttt atccaccacg tcatcaggca ttcgggtaga
     8521 aatcatggcg gtaaagttag cgacaatctc tgtcaactta ttcactgcct gttgcttatt
     8581 actttcgctc atcatggcaa ctccagtttt tgtatttgtc tggaaaaatt caccgccaca
     8641 aactaacact aaaaatgtgg ttttatattt tttgccaccg ttaatatgta attaacagtt
     8701 cgtgaatcaa cgcgaaaata agaatgaata ttgcgccaga gcagggaatg gcaatgtttt
     8761 gcgaaataca ctgcgcttat cacaactgcg gataattaac gatgctgaat agctggcctt
     8821 tagccaaaga cttgcaggta ctggtagaaa tcgtccacag cgggagtttt agtgctgcag
     8881 cggcgacgct tgggcaaacg cctgcatttg tcaccaagcg gatccaaatc cttgaaaata
     8941 cgctggcaac aacgctgctg aaccgctccg ctcgcggcgt ggcgctgact gaaagtggcc
     9001 aacgttgtta cgaacatgcg ctggaaatcc tcacccaata tcagcgactg gtcgatgacg
     9061 tcacgcaaat caaaacgcgc ccggagggga tgattcgtat tggttgcagt tttggttttg
     9121 ggcgcagcca tattgcgcca gccattaccg aacttatgcg caattatcct gagttacagg
     9181 tgcattttga attgttcgat cggcaaattg atttagtgca ggataatatt gatctggata
     9241 ttcgtattaa tgacgaaatt cctgattatt atattgcgca tctgttaacg aaaaataaaa
     9301 gaatattatg cgcagcacct gagtatctgc aaaaatatcc gcagccacaa tcattacagg
     9361 aattaagtcg tcatgactgt ctggtgacca aagagcgcga tatgacccac ggaatatggg
     9421 agttggggaa cggtcaggag aaaaaatcgg tgaaggttag cgggcatctt tcctccaata
     9481 gcggcgagat agtgttgcaa tgggcgctgg agggcaaagg gattatgctg cgttccgagt
     9541 gggatgtgtt gccgtttctg gagagtggca aactggtgca agtattgccg gagtatgcac
     9601 aaagcgccaa tatctgggct gtttaccggg agctgctcta tcgcagcatg aaattcgtgt
     9661 ctgcgcggaa tttctggcgg catggtgcca gcaacggctg ggcaagcccg atgaaggcta
     9721 tcaggtcatg tagatccacc agaaatcact cgggatcctt ttcgcgcttt cttttgaatt
     9781 tcgtccagat ttttgtctcc tgacgacgcc acagacgctg gatgttgtca tgatgacgca
     9841 gcaggatcag gcaagagagc atcgaaaccg ggaaggtgaa ttgtggctta aaccaccaga
     9901 cataaaacgg agcaatcagt gcactgacaa tcgctcccag cgacgagtat ccgctcaata
     9961 gcacggtcag taaccaggtt cccgccatta ctccggtgag atcccagcta attggtgcga
    10021 ttgctccaaa agcggtagca acgccttttc ctcctttaaa accgaagaaa acgggccaga
    10081 tgtgtccaag acaggcggcg atggcaatta agcctagcca gaaggggctg acacctaatt
    10141 catacgcgcc ccagacaggc aacattcctt tcagaacgtc gaaaatcagt actgctacgg
    10201 ctgctccctt gccaccgata cgtaacacat tggttgcgcc tggattgccg gagccgctgg
    10261 ttcgcggatc gggcagccca cacaagcggc aaaccagaat ggcactggaa atggagccgc
    10321 agaggtacgc gatgaggatc attccaggcg cgattgcact cataagctgt tccgttttga
    10381 aaattcgtgt tcaacgatga atctgtggat aatacgcaca tttcgccgga agtggtatcc
    10441 ggttagccaa aaagcaggca ggacgtgatg gatattgtat ttatagagca actttcggta
    10501 atcaccacta ttggtgttta cgactgggaa cagaccatcg aacagaagtt agtgttcgat
    10561 atcgaaatgg catgggataa ccgtaaagcg gcgaaaagtg atgatgtggc ggattgcctc
    10621 agttacgctg acattgcaga aacggtggtc agccacgtcg agggggcgcg ttttgcgctg
    10681 gtggaacgcg tggctgaaga ggtggcggag ctgctgttag cacgcttcaa ctcgccgtgg
    10741 gtgcgtatca aactcagcaa gccaggcgca gtggcgcggg cggcgaatgt tggcgtaatc
    10801 attgagcgtg gcaataatct gaaagaaaat aattaatttt acagctgtta aaccaaacgg
    10861 ttataacctg gtcatactgc agtagttcgg acaagcggta cattttaata atttaggggt
    10921 ttattgatga gcgatatgca ctcgctgctg atagcggcaa tattgggtgt ggtcgaagga
    10981 ttgacagaat ttctgccggt atccagcacg ggccatatga ttattgtcgg tcacttgttg
    11041 gggtttgagg gcgacacggc gaaaaccttt gaagttgtga tccagttagg atcaattctg
    11101 gcggtagtag tgatgttctg gcggcgtctg tttggcctga ttggcatcca ctttggccgc
    11161 ccgttgcagc acgaaggtga aagcaaaggt cgtttaacgc tgatccacat tttgctgggg
    11221 atgattccgg cggtggtact ggggctatta ttccacgaca cgattaagtc attgtttaac
    11281 ccgataaatg tgatgtatgc gctggtcgtt ggcggtttgt tgctgattgc cgccgaatgc
    11341 ctgaagccga aagagccgcg tgcgccgggt cttgatgata tgacctatcg tcaggcgttt
    11401 atgattggct gtttccagtg tctggcgctg tggccgggtt tctcccgttc cggggcgacc
    11461 atttcaggtg ggatgctgat gggcgtgagc cgttacgctg cttccgagtt ctcgttcctg
    11521 ctggcggtac cgatgatgat gggcgcaacg gcgctcgatc tctacaaaag ctggggcttc
    11581 ctgacaaccg gcgatatccc gatgtttgcc gttgggttta tcaccgcttt tgtggtggcg
    11641 ctgatagcga ttaaaacctt cctgcaattg attaagcgca tttcgtttat cccgttcgcc
    11701 atttatcgct ttattgtggc ggctgcggtg tatgtcgtgt tcttttgatt gagaaaaggt
    11761 tcgggagcag gccgggtttt ctccctcgct ctgtagggag agggccgggg tgggggtgat
    11821 tgttcgcacc ggtgctggtc tgttcccctc accctaaccc tctccccgaa ggggcgaggg
    11881 gacggttcgt gctaacgttt agttttatac tcagtcaggc tttgggcaac gttgttcctt
    11941 ccagctggct accgccgcaa tccgccgtcg ggtcagctcc tcgcgaatct ccacgccttt
    12001 aaatcccgct tcaacgaccg cttttgtcgg cactgactgc gccacttccc agccttcgcg
    12061 caaccaacgg ccttgcgggt agtccgctga ttcaaaaccg gttctgccgc gcacgtcagc
    12121 ctcgctggtc agcgccagtt gctcgacacg ctgcggttta cgccaggcat cgatggaatc
    12181 aaataatttg acgatggttt ttgggttcag cattgggaag gtgcggatga gatcgtgaaa
    12241 ctcagccacc agtctggcta aatcgcgaat ttcatttggc acgcgcagac gctggcatag
    12301 ttgttccact aacttaacac ccgccgggcc atgcccatga tgacgcggcc aaagttctgg
    12361 cggcgtcagt cctttaccga gatcgtggca taaagttgcg aaacggacat caacctgcgg
    12421 actcagcatc gccgccattg agagcgtcat taaggtatga atacccgtgt cgatttccgg
    12481 atgccacctg gcaggtgccg gaacgccaaa cagtgcgtca atttccggga ataaaacgcg
    12541 aagtgcgccg caatcgcgca gtacctggaa gaacacctgg ggattgcggg tggtgagggc
    12601 gctttccgtc tctttccata cccgttcagg cgtcaggtgt tccagttcac ccgcatgggt
    12661 catctcgcgc atcagcgtca gagtttcatc ggcaatacga aaaccgaggt gggcataacg
    12721 cgcagcaaaa cgcgccacgc gcagtacgcg taacggatct tcgccaaaag cgggggaaac
    12781 atggcgcaac agacgatttt gcagatcgcc cagaccgttg tacgggtcga taatctcgcc
    12841 gttatcgtcc tgggccagcg cattaatggt cagatcgcga cgcttaagat catcttccag
    12901 cgtgacatcc ggtgcggtat agcaagtaaa accggtgtaa ccggaaccgg atttccgttc
    12961 ggtacgtgcc agcgcatact cttcatgcgt ttgcggatgc agaaacacag gaaaatcgcg
    13021 gcctacctgc tggtagcccg cgtcgagcat ctcctgtggc gtactgccga ccaccaccca
    13081 atctctgtct ttgaccgata gtcctaacaa tgcatcccga acagcaccac cgaccagata
    13141 aatcttcacg ccatcatccc ttcgtcaaat acacaattcc taataataag acagtgcaac
    13201 gaagaaggcg atttagttca tccagcgatc tttgcgtttg cggcttggga tcaggtgcgg
    13261 cagtaccaga ccgagcagca agccaagccc cagcacgcca ccgccataca taaaccattg
    13321 catgatgatg gtgcgctgtt tgtcatccag ctgtacgctg gcggcatcga cctttttctg
    13381 cgcgacaatc agctcgtttt tcagtttctg attttcttct tttaacccgt tgatcacgct
    13441 gtcgctctgc gccacttttt gctgcatttc tgcggtgcgc tgattccagg tgttatcgat
    13501 attggtgagt ttatcggtca gggttttgac ctgattttcc agatctggca cacgggagcg
    13561 caggcttggc tcagtgctaa gttgtttcaa cgggatccag gcggtacggc cagagctgtc
    13621 tttcacctgg gcataattgg tgttggcgtc agtttgtaat aaggtcactt cctcgccggc
    13681 gttaaccgtg cccacgaggc gataatgatc tcccggaccg ctacggaccc aggtattcag
    13741 ttcgtcagaa acatagcgcg tttcttcagc gtgtgagacg gcagtcgcgc taagtgcgag
    13801 taaagttaat ccgatcaggc gtaattttgg catcaggctg tcgttattgt catggaaagt
    13861 ggaacgatag tagtggcatc agtgtcgcta cgcaaagcat tcgacatcaa tcggaatcct
    13921 ctgtgtcagg tcttgccact gcttcaaact tttacgcccg tcaaattgcc cgttgcatgg
    13981 caatttggcg caaaatacta tctactgaca aaaaagatcg cattaagttc gccgttcatc
    14041 gtcacttatg tgacataacc gataagtaaa ggccatggct caggaaatcg aattaaagtt
    14101 tattgttaat cacagtgccg ttgaggcgtt gcgtgaccat ctcaatacgc tgggcggcga
    14161 gcaccatgac cccgtgcagt tgctgaatat ttactacgaa acgccggata actggctgcg
    14221 taggcacgat atgggcttac gtattcgtgg cgaaaacggt cgctatgaga tgaccatgaa
    14281 agttgcagga agagtgacag gcggcttaca ccagcgcccg gaatataacg tggcgttgag
    14341 cgaaccgacg ctcgacctgg cgcagttacc gacggaaatc tggccgaacg gcgaattgcc
    14401 tgccgatctc gcttcccgcg tgcagtcgct gtttagcacc gatttttatc gcgaaaaatg
    14461 gctagtagag atcgatggca gccgaattga aatcgctctc gaccaggggg aagtgaaagc
    14521 gggtgaattt gctgagccta tctgcgagct ggaactggag ctgcttagcg gcgacacgcg
    14581 cgcggtgctg aaactggcga accaactggt atcgcaaacc ggattacgcc agggcagcct
    14641 gagcaaagcg gcgcgtggct atcatctggc gcagggcaat ccggcgcgtg aaatcaaacc
    14701 gaccaccatt ttgcatgttg cggcaaaagc cgatgtggaa caggggctgg aagcggcgct
    14761 cgagctggtg ttagcgcaat ggcagtatca tgaagaactg tgggtacgcg gcaacgatgc
    14821 ggcgaaagag caggtgctgg cagccattag cctagtccgt catacactga tgctgttcgg
    14881 tggcattgtg ccgcgtaaag cgagtactca cttacgtgat ttgctgactc aatgcgaagc
    14941 gaccatcgct tctgcggttt ctgccgtgac ggcggtctac tctaccgaaa cggcaatggc
    15001 gaagctggcg ttgaccgaat ggctggtaag caaagcatgg cagccgtttt tagatgccaa
    15061 agcgcagggc aaaattagcg actccttcaa acgctttgcc gatatccatc tttcccgcca
    15121 tgccgctgaa ctgaaaagcg ttttctgcca gccgttaggc gatcgctacc gtgaccagtt
    15181 gccacgcctg atgcgtgata ttgactcaat actgttgctg gcgggttact atgatcctgt
    15241 cgtcgcgcaa gcctggctgg agaactggca ggggctgcgt cacgctattg cgaccgggca
    15301 acgcattgaa attgaacatt tccgtaatga ggcaaacaat caggaaccgt tctggttgca
    15361 cagcggaaaa cgttaatcag gcaaggataa aatcgcttat gaagccgctc tcttcaccgt
    15421 tacagcagta ctggcagacc gttgttgagc ggctgccaga gcttttagcc gaggaatcac
    15481 ttagtgcaca ggcgaagtca gtacttactt tcagtgattt tgtgcaggac agcgtgattg
    15541 cgcatccaga gtggctgacg gaactggaaa gccaaccgcc acaggctgac gaatggcaac
    15601 attacagcgt gtggttgcag gaggcgctca gtaatgtgag cgacgaagcc gggttaatgc
    15661 gcgagctgcg gctattccgg cggcgcatta tggtgcgcat cgcatgggcg caaacgctgg
    15721 cactggttac tgaagagagc atattgcagc agctcagtta tctggcggag acgctgattg
    15781 ttgcggcgcg tgactggctg tatgacgcct gctgccgcga gtggggaacg ccgtgcaatg
    15841 cgcagggcga agcgcaaccg ctgctgatct taggcatggg taagctgggc ggcggggaac
    15901 tgaatttctc ctctgatatc gatctgattt ttgcctggcc ggaacatggt tgtacgcagg
    15961 gcggacgccg ggaactggat aacgcgcagt tttttacccg catggggcag cggctgatta
    16021 aagtgctgga tcaaccaacg caggatggct tcgtctatcg cgtggatatg cgactgcgtc
    16081 cgtttggcga aagtggcccg ctggtgctga gctttgccgc gttggaggat tattaccagg
    16141 agcagggacg cgactgggag cgttacgcga tggtcaaagc gcggattatg ggcgatagcg
    16201 aaggcgtcta tgctaacgag ttgcgtgcga tgctgcgccc gtttgttttc cgtcgttaca
    16261 tcgatttcag cgtgattcag tcgctgcgca acatgaaagg gatgattgcc cgtgaagtgc
    16321 gtcgacgtgg tttgaccgac aatatcaaac tcggcgcagg cggcattcgc gaaattgaat
    16381 ttatcgttca ggtgttccag ctcattcgcg gtgggcgcga accgttgctg cagtcgcgct
    16441 ctttactgcc aacgctcagc gccattgccg cgctgcatct gctttctgaa aacgatgctg
    16501 aacaattgcg agtggcatat ctgttcctgc ggcgtctgga aaacctgctg caaagcatta
    16561 acgacgaaca aacccaaacg cttccttctg atgagcttaa ccgtgcgcgg ctggcgtggg
    16621 cgatggactt tgctgactgg ccgcaactga ccggggcgct gaccgcacat atgaccaatg
    16681 tgcgccgggt gtttaatgaa ttgattggcg acgacgaaag tgaaactcag gaagagtcgc
    16741 tgtcggaaca gtggcgtgag ctgtggcagg atgcgttgca ggaagatgac actacgccag
    16801 tgctggcgca tcttagcgag gatgatcgca aacaggtcct aacgatgatt gctgatttcc
    16861 gcaaagagct ggataagcgt actatcgggc cgcgaggacg tcaggtgctc gaccatctga
    16921 tgccacatct gctaagtgat gtctgtgcgc gtgaagacgc tgctgtcacg ctgtcgcgca
    16981 ttaccgcctt gctggtgggg attgttaccc gcacaaccta tttagaattg ctcagtgaat
    17041 tccctgcggc gcttaaacat ttgatttctc tgtgtgccgc atcgccgatg attgccagcc
    17101 agctagcgcg ttatccatta ttgctggatg aattgctcga tccaaatacc ctttatcagc
    17161 cgacggcgac cgatgcctac cgtgatgaac tgcgccagta tttgctgcgc gtgccggaag
    17221 atgacgaaga acaacagctt gaggcgctgc gtcagttcaa acaggcgcag ctgttacgca
    17281 ttgcggcagc ggatatcgcc ggtacgctac cggtaatgaa agtgagcgat cacttaacct
    17341 ggctggcaga agctatgata gatgccgtcg ttcagcaggc gtggggtcaa atggtggccc
    17401 gctacggtaa gccaaatcac ctcaacgaac gtgaagggcg cggttttgcg gtggtcggct
    17461 acggcaagct gggcggctgg gagttaggct acagttccga tcttgacctt atcttcctcc
    17521 atgattgccc aatggatgcg atgactgacg gcgagcggga aatcgacggg cggcagtttt
    17581 atctgcgtct ggcgcaacgc attatgcatc tgttcagtac gcgtacctct tccggcattt
    17641 tgtatgaagt ggatgctcga ctgcgtccgt ccggggcggc gggaatgctg gtgacatccg
    17701 cagaagcatt tgccgattat cagaaaaacg aggcctggac gtgggaacat caggcgctgg
    17761 tgcgtgcgcg tgtagtgtac ggcgatccgc agctcactgc gcactttgac gcggtgcgtc
    17821 gtgagattat gacactgccg cgagaaggta aaactctgca aacggaagtg cgggaaatgc
    17881 gcgagaaaat gcgcgctcat ctcggcaata aacatcgtaa tcgctttgat atcaaagccg
    17941 atgaaggcgg aattaccgat atcgaattta ttacccaata tctggtgttg cgctacgctc
    18001 atgaaaaacc gaagttaacg cgctggtcag acaacgtgcg tattctggag ctgctggcgc
    18061 aaaacgacat tatggaagag caggaagcga tggcgctgac ccgtgcttac acgacgctgc
    18121 gcgatgaact tcatcatctg gcattacagg aattgccggg ccatgtgtcg gaggattgct
    18181 tcaccgcaga gcgtgatctg gtgcgggcaa gctggcagaa gtggctggtg gaagaatgaa
    18241 gtatggtatt atcgcgcgca aattttgaat ctctcaggag acaggaatga aagtaacgct
    18301 gccagagttt gaacgtgcag gagtgatggt ggttggtgat gtgatgctgg atcgttactg
    18361 gtacggcccc accagtcgta tctcgccgga agcgccggtg cccgtggtta aagtgaatac
    18421 catcgaagaa cgtccgggcg gcgcggctaa cgtggcgatg aatatcgctt ctctcggtgc
    18481 taatgcacgc ctggtcgggt tgacgggcat tgacgatgca gcgcgcgcgc tgagtaaatc
    18541 tctggccgac gtcaacgtca aatgcgactt cgtttctgta ccgacgcatc cgaccatcac
    18601 caaattacgg gtactttccc gcaaccaaca gctgatccgt ctggattttg aagaaggttt
    18661 cgaaggtgtt gatccgcagc cgctgcacga gcggattaat caggcgctga gttcgattgg
    18721 cgcgctggtg ctttctgact acgccaaagg tgcgctggca agcgtacagc agatgatcca
    18781 actggcgcgt aaagcgggcg ttccggtgct gattgatcca aaaggtaccg attttgagcg
    18841 ctaccgcggc gctacgctgc tgacaccgaa tctctcggaa tttgaagctg ttgtcggtaa
    18901 atgtaagacc gaagaagaga ttgttgagcg cggcatgaaa ctgattgccg attacgaact
    18961 ctcggctctg ttagtgaccc gttccgaaca gggtatgtcg ctgctgcaac cgggtaaagc
    19021 gccgctgcat atgccaaccc aggcgcagga agtgtatgac gttaccggtg cgggcgacac
    19081 ggtgattggc gtcctggcgg caacgctggc agcgggtaat tcgctggaag aagcctgctt
    19141 ctttgccaat gcggcggctg gcgtggtggt cggcaaactg ggaacctcca cggtttcgcc
    19201 gatcgagctg gaaaatgcag tgcgtgggcg cgcagatacc ggctttggcg tgatgaccga
    19261 agaggaactg aagctggccg tagcggcagc gcgtaaacgc ggtgaaaaag tggtgatgac
    19321 caatggcgtc tttgacatcc tgcacgccgg acacgtcttc tatctggcaa atgcccgcaa
    19381 actgggtgac cgcttgattg tcgctgtgaa cagcgatgcc tccaccaaac ggctgaaagg
    19441 ggattctcgc cccgttaacc cgctcgaaca gcgtatgatt gtgctgggcg cactggaagc
    19501 ggtcgactgg gtggtgtcgt ttgaagaaga cacgccgcag cgcttgatcg ccgggatcct
    19561 gccagattta ctggtgaagg gcggcgacta taaaccagaa gagatcgccg ggagtaaaga
    19621 agtctgggcc aacggcggcg aagtgctggt gctcaacttc gaagacggtt gctcgaccac
    19681 taacatcatc aagaagatcc aacaggataa aaaaggctaa ccggaaagcg gttcacagat
    19741 ctcgcttcgt agcaggcagg cctattgacg ctgttgtttt ttcaacctaa agtaaaggaa
    19801 caagggtaag ggaggatttc tcccccctct gatcagttgt tagtaagtcg ggaaacttaa
    19861 cagtaacaac
//
LOCUS       DAETQL010000075        19377 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.075, whole genome shotgun sequence.
ACCESSION   DAETQL010000075 DAETQL010000000
VERSION     DAETQL010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19377
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.075"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="K0W65_002926"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="K0W65_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBI3096113.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="K0W65_002927"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="K0W65_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HBI3096114.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="K0W65_002928"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="K0W65_002928"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBI3096115.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTLSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="K0W65_002929"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="K0W65_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBI3096116.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="K0W65_002930"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="K0W65_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBI3096117.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="K0W65_002931"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="K0W65_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBI3096118.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="K0W65_002932"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="K0W65_002932"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBI3096119.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="K0W65_002933"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="K0W65_002933"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBI3096120.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="K0W65_002934"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="K0W65_002934"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBI3096121.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="K0W65_002935"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="K0W65_002935"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBI3096122.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="K0W65_002936"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="K0W65_002936"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBI3096123.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="K0W65_002937"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="K0W65_002937"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBI3096124.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="K0W65_002938"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="K0W65_002938"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBI3096125.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="K0W65_002939"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="K0W65_002939"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBI3096126.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="K0W65_002940"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="K0W65_002940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBI3096127.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="K0W65_002941"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="K0W65_002941"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBI3096128.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="K0W65_002942"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="K0W65_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBI3096129.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="K0W65_002943"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="K0W65_002943"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBI3096130.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         4772 a         4856 c         5118 g         4631 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cagggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaata atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggct ggcgttatca tccgatcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggag gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagat aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctag cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgccgac
//
LOCUS       DAETQL010000076        18486 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.076, whole genome shotgun sequence.
ACCESSION   DAETQL010000076 DAETQL010000000
VERSION     DAETQL010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.076"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..193
                     /locus_tag="K0W65_002944"
     CDS             <1..193
                     /locus_tag="K0W65_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3096131.1"
                     /translation="LYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLVRKSLSFSK
                     SVELHDKVIGHYLNIKHYQ"
     gene            469..993
                     /locus_tag="K0W65_002945"
     CDS             469..993
                     /locus_tag="K0W65_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI3096132.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            1074..1748
                     /locus_tag="K0W65_002946"
     CDS             1074..1748
                     /locus_tag="K0W65_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBI3096133.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1764..4244
                     /locus_tag="K0W65_002947"
     CDS             1764..4244
                     /locus_tag="K0W65_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI3096134.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            4260..5293
                     /locus_tag="K0W65_002948"
                     /pseudo
     CDS             4260..5293
                     /locus_tag="K0W65_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5375..5713)
                     /gene="rcnB"
                     /locus_tag="K0W65_002949"
     CDS             complement(5375..5713)
                     /gene="rcnB"
                     /locus_tag="K0W65_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBI3096135.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5932..6762)
                     /gene="rcnA"
                     /locus_tag="K0W65_002950"
                     /pseudo
     CDS             complement(5932..6762)
                     /gene="rcnA"
                     /locus_tag="K0W65_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6884..7156
                     /gene="rcnR"
                     /locus_tag="K0W65_002951"
     CDS             6884..7156
                     /gene="rcnR"
                     /locus_tag="K0W65_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HBI3096136.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      7248..7344
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7379..8167
                     /gene="thiM"
                     /locus_tag="K0W65_002952"
     CDS             7379..8167
                     /gene="thiM"
                     /locus_tag="K0W65_002952"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBI3096137.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            8164..8964
                     /gene="thiD"
                     /locus_tag="K0W65_002953"
     CDS             8164..8964
                     /gene="thiD"
                     /locus_tag="K0W65_002953"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBI3096138.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            9029..9850
                     /locus_tag="K0W65_002954"
     CDS             9029..9850
                     /locus_tag="K0W65_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBI3096139.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9898..10644
                     /locus_tag="K0W65_002955"
     CDS             9898..10644
                     /locus_tag="K0W65_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBI3096140.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10618..11583)
                     /locus_tag="K0W65_002956"
     CDS             complement(10618..11583)
                     /locus_tag="K0W65_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBI3096141.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11580..12583)
                     /locus_tag="K0W65_002957"
                     /pseudo
     CDS             complement(11580..12583)
                     /locus_tag="K0W65_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12623..13675
                     /gene="fbaB"
                     /locus_tag="K0W65_002958"
     CDS             12623..13675
                     /gene="fbaB"
                     /locus_tag="K0W65_002958"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBI3096142.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13983..14837
                     /gene="gatY"
                     /locus_tag="K0W65_002959"
     CDS             13983..14837
                     /gene="gatY"
                     /locus_tag="K0W65_002959"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBI3096143.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14866..16128
                     /gene="gatZ"
                     /locus_tag="K0W65_002960"
     CDS             14866..16128
                     /gene="gatZ"
                     /locus_tag="K0W65_002960"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HBI3096144.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGHALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            16138..16590
                     /gene="gatA"
                     /locus_tag="K0W65_002961"
     CDS             16138..16590
                     /gene="gatA"
                     /locus_tag="K0W65_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBI3096145.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16621..16905
                     /gene="gatB"
                     /locus_tag="K0W65_002962"
     CDS             16621..16905
                     /gene="gatB"
                     /locus_tag="K0W65_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBI3096146.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16909..18264
                     /locus_tag="K0W65_002963"
     CDS             16909..18264
                     /locus_tag="K0W65_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBI3096147.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            18359..>18486
                     /locus_tag="K0W65_002964"
     CDS             18359..>18486
                     /locus_tag="K0W65_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3096148.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQ"
BASE COUNT         4837 a         4207 c         4675 g         4767 t
ORIGIN      
        1 gctgtatgaa tcacgcctga agggaaagct gcacgttatc agcaagcgtt acactcagcg
       61 cattgagcga cataatctga atctgagaca acatctggca aggctggtac ggaagtcact
      121 gtcgttctca aaatcggtgg agctgcatga caaggtcatc gggcattatc tgaacataaa
      181 acactatcag taagttggag tcattaccga cgaaatcatt tatataaatg atttcgtctt
      241 ttttattgat atcaacaata ccatttacat aaatctacat taattaatag atgtgataaa
      301 tatttaatat tcttaagtat ttgttgtatt tatattgcat tggttaattt gttttaaatc
      361 aataaaacaa tatgtttaca ttgcggcagc ttttttgatg tgagaaattt gtttccaaga
      421 gatacagact cttaacaacc aaaaataaat tttattaagg aacatattat gaaacgttca
      481 attattgctg ccgctgtctt ttcttctttt tttatgagcg ctggagtatt tgctgcagac
      541 gttgataccg gaacattaac cattaaagga aatattgcag aatctccgtg taaattcgaa
      601 gcgggtggtg attcagtaag tattaatatg ccgactgtac caaccactgt ctttgaaggt
      661 aaagctaaat attctaccta tgatgatgca gtcggtgtaa ccagcagcat gttaaaaatt
      721 agctgcccga aagaagttgc tggtgtaaaa ctctcgttga tcacaaacga caaaataacc
      781 ggtaacgata aggcgatagc cagtagcaac gataccgtag gtgataacag cgatgtccta
      841 gatgtttctg caccttttaa cattgagagt tataaaacag cggaaggtca atatgctatt
      901 ccgtttaaag caaaatacct gaaactgaca gataactcag tgcaatcagg tgatgtgtta
      961 tcttctctgg ttatgcgtgt ggcgcaggat taataaataa aattgcaagt ttctccaggt
     1021 aataaacatg gccgctatcc ccatggcggc tttttaattt aagaggcatt aagatgaaag
     1081 gattattatc tttactcatt ttttctatgg tccttcctgc acatgccgga attgttatct
     1141 acgggacgcg cattatttac ccggcagaaa ataaagaagt tatggtgcag ttgatgaacc
     1201 agggaaatcg ttcttcgctg ctgcaggcgt ggattgatga tggcgatacg tcgttaccac
     1261 cagaaaaaat tcaggttcct ttcatgttaa cgccaccagt ggcaaaaata ggggcaaatt
     1321 ccgggcagca agtaaaaatc aaaattatgc cgaataaact gcccactaat aaagaaagca
     1381 ttttttatct gaatgttctg tatattccac caaatagtcc agagcaagaa ggcaagaatg
     1441 cactgaagtt tgcgatgcaa aacagaatta agttgtttta ccggccagcg ggtattgctc
     1501 cggtaaataa agcgaccttt aaaaagttac tggtaaatcg cagtggcaat ggattggtga
     1561 taaaaaatga ctcagctaat tgggtgacga tttcggatgt caaagctaat aatgttaaag
     1621 tcaattatga aactattatg attgccccct tagaaagtca gagtgttaat atcaaaagta
     1681 ataatgcaaa taactggtat ctgaccatta tcaatgacca cggcaactat attagtgaca
     1741 aaatttaatt gcaggagctg cctatgttga gaatgacccc acttgcatca gcaatcgtag
     1801 cgttattgct cggcattgaa gcttatgcag ctgaagaaac ctttgatacc cattttatga
     1861 taggtggaat gaaagaccag caggttgcaa atattcgtct tgatgataat caacccttac
     1921 cggggcagta tgacatcgat atttatgtca ataagcaatg gcgcgggaaa tatgagatta
     1981 ttgttaaaga caacccgcaa gaaacatgtt tatcaagaga agttatcaag cggttaggca
     2041 ttaatagcga taacttcgcc agcggtaagc aatgtttaac atttgagcaa cttgttcagg
     2101 gtgggagcta tagctgggat atcggggttt ttcgtctcga tttcagtgtc ccgcaggctt
     2161 gggtggaaga actggaaagt ggctatgttc caccggaaaa ctgggagcgg ggtattaatg
     2221 cgttttatac ttcttattat gtgagtcagt attacagcga ctataaagcg tcgggtaaca
     2281 acaagagtac atatgtacgt tttaacagcg ggttaaattt actggagtgg caactgcatt
     2341 ctgatgccag tttcagtaaa acaaataaca atccaggggt gtggaaaagc aataccctgt
     2401 atctggaacg tggatttgcc caatttctcg gcacgcttcg cgtgggtgat atgtacacat
     2461 caagcgatat ttttgattct gttcgcttca gcggtgtgcg gttgtttcgt gatatgcaga
     2521 tgttgcctaa ctcgaaacaa aattttacgc cacgggtgca ggggattgct cagagtaacg
     2581 cgctggtaac tattgaacag aatggttttg tggtttatca gaaagaggtt cctcctggcc
     2641 cgtttgcgat tacagatttg cagttggccg gtggtggagc agatcttgat gttagcgtga
     2701 aagaggcgga cggctcggta accacctatc tggtgcctta tgcagcggtg ccaaatatgc
     2761 tgcaacccgg cgtgtcgaaa tatgattttg cggcgggccg tagccatatt gaaggggcga
     2821 gcaagcaaag tgattttgtc caggcaggtt atcagtatgg ttttaataac ttattgacgc
     2881 tgtatggcgg cacgatggtt gctaataatt actatgcgtt taccctcgga acgggttgga
     2941 acacgcgcat tggcgcaatt tccgtcgatg ccacgaagtc gcatagtaaa caagacaacg
     3001 gcgatgtgtt tgacgggcaa agttatcaaa ttgcctacaa caaatttgtg agccaaacgt
     3061 cgacgcgttt tggtctggcg gcctggcgtt attcgtcgcg tgattaccgg acatttaacg
     3121 atcacgtttg ggcaaacaat aaagataatt atcgccgtga tgaaaacgat atctatgaca
     3181 ttgccgatta ttaccagaac gattttggcc gtaaaaatag cttttctgcc aatatgagcc
     3241 agtcattgcc agaaggctgg ggttctgtgt ccttaagtac gttatggcga gattactggg
     3301 ggcgtagcgg cagcagtaag gattatcagt tgagttattc caataactgg cggcggataa
     3361 gctataccct cgcggcaagc caggcgtatg gcgagaatca tcatgaagag aaacgtttta
     3421 atatttttat atcaattccc tgtgattggg gtgatgacgt tacgacgcct cgtcggcaaa
     3481 tatatatgtc taactcaacg acgtttgatg atcagggttt tgcctcaaat aatacgggat
     3541 tatcaggaac agtagggagt cgggatcagt tcaattatgg tgtcaacctg agtcatcaac
     3601 atcagggaaa tgaaacgaca gctggggcga atttgacctg gaacgcgccg gttgcgacag
     3661 tgaatggcag ttatagtcag tcgagtactt atcgacagac tggagccagt gtttcagggg
     3721 gcattgtcgc ctggtcgggt ggcgttaatc tggcgaaccg tctttccgaa acgtttgctg
     3781 tgatgaatgc gccaggaatt aaagatgctt atgtcaatgg gcaaaaatat cgcacaacaa
     3841 accgtaatgg agtggtggta tacgacggaa tgacacctta tcgggaaaat cacctgatgc
     3901 tggatgtgtc gcaaagcgat agcgaagcag aattacgtgg caaccggaaa attgccgccc
     3961 cttatcgcgg cgcggttgta ctggttaatt ttgataccga tcagcgcaag ccctggttta
     4021 taaaagcgtt aagagcggat ggtcaaccat taacgtttgg ttatgaagtc aatgatatcc
     4081 atggtcataa tattggcgtt gtcggccagg gaagccagtt atttattcgc accaatgaaa
     4141 taccgccatc ggttaatgta gcaattgata agcaacaagg actttcatgc acaatcacct
     4201 tcggtaaaga gattgatgaa agtagaaatt atatttgcca gtaatagtca ggtggttcta
     4261 tggaaattcg cataatgcta tttatattaa tgatgatggt tatgcctgtg agctatgcgg
     4321 catgttatag tgagttatct gttcagcaca atttggttgt tcaggggaat tttgcactta
     4381 ctcaaacaca aatagcgaca tatgagcata attttaatga ttcgtcatgc gtgagtacaa
     4441 atactatcac ccctatgagc ccatcggata ttattgttgg actttataac gataccatta
     4501 aattaaattt acattttgaa tggaccaata aaaacaacat cacgttgtca aataatcaga
     4561 gcagtttcac cagtggttat tcggtaacgg tgacacctgc ggccagtaat gcaaaagtga
     4621 atatttctgc ggggggcggt ggttcagtga tgattaatgg tgttgcgaca ttatccagtg
     4681 cttcatcatc gacacgcggg agtgccgcag tacaatttct actgtgttta ttaggtggca
     4741 agtcatgggg tgcatgtgta aatagctaca gaaatgcatt ggcacaaaat gcaggtgtct
     4801 attccttcaa tctgacattg tcatacaacc cgataaccac aacctgcaaa caggacgatt
     4861 tattaattac tttagacagt attcccgttt tacaattacc agccacaggt aacaaagcaa
     4921 caataaatag taaaaaaagg gatattattc tgcgttgtaa aaatttatta ggtcaacaaa
     4981 atcaaacatc acggaaaatg caggtgtatt tatcaagttc tgacttgtta accaacagca
     5041 acacaatact gaaaggtgcg gaagataatg cgtaggattt attcttgaaa gtaatggttc
     5101 gccagtcaca cttttaaata tcactaacag cagtaaagga tacacaaatt taaaagaaat
     5161 tgcggcaaag tcaaaactta cagatacaac ggtttcaatt ccgataacag ccagttacta
     5221 cgtctacgat acaaacaaag ttaaatctgg cgcactggag gcaaccgcat taatcaacgt
     5281 gaaatacgac taataacaag caaaacgagc aagtggctaa taataaaaaa taacaaggtg
     5341 tctgcatttc cccctcatga tgagggggct ttttttagcg atgataaaaa atctcaccgt
     5401 cgtaggcttt aatgatttta ccgtcggtgt cgctgatcaa cacgtacgcg ccacccatat
     5461 aggtccagtg cgtcccggca tcaggcgcgg gcaggttacg taactgccac tgcttaatgt
     5521 tgtactcatc ggtgcggtac atctctggga cggtatcacc tggtttgaaa tgcttaaagt
     5581 cagcaatgaa actacttaac tcatacttag aaattcccga ggtattggtc gaacccgcgg
     5641 tggctggtgc ggcccaggcg gcactggtaa ccagcaaaag cacacccagc aacatcttat
     5701 ttttaatggt cataaactct ccactttggg gatctgtaat atcagtcgca tatctttcct
     5761 gtcatacaat ataactgaca agcgatattt tgttagtgat cccatgattt cgtaaaaagt
     5821 gtaaatgaaa ggatgcccta aaagcatcct tagcgagcag agagtcagcg gaaatagctg
     5881 atttaagaat catcctgggc tgaagataac atgcaagcct aaaggattga gttatcgcat
     5941 tatgcccatg aagccatgta cgcccatata cacaccgact aagccaatca acagactgga
     6001 aaaatagggg gcgcgtttag cgagagtgtt aaatccgctc cagcgttttg cgacctgctg
     6061 aacgctgatt gctgcgccaa cgcctacggt gacaagcgtt aacgccaggc caatgctgaa
     6121 actgacgacc agtgttgcgc ccagtgtcag ggctttcaac tgaatgcata tcaacagcac
     6181 ggtaattgct gccgggcagg ggataaagcc accggttaag ccaaataaca aaatttgcca
     6241 gttgatgacc tctctaccat caaagcgtcg tttaatgtca ttggcatggg ctcgtgcatg
     6301 ggcatcctga tactcgccat gttcgtgatg gtgatgatgt ccttggtcgt ggtgatgttc
     6361 gtgatcgtga tgatggtgat gatgctcata atcatgcccg tgcatattct ccagccagtt
     6421 gcgttcgccg cgctaggtac gccagaacat ccagaacgcg gtgccaataa tgatcactgc
     6481 ggaaatcagc tggagccacg gttctgctga ttgagcagta aagcgcttac tgatcaccat
     6541 cccgccaaag gcaatcaacc agaccaccgc ggtatgcgaa atggttgctg ccagtccgag
     6601 catcaccgcc tgtttaatgg tgcctttgat ggcgatgata aacgccgcca tcatcgtttt
     6661 tgagtgccct ggttccaggc catgaagcgc accaagtaag atggcgctgg ggatgaagaa
     6721 ccaggcgttt ccttgctgaa gaagagttgt aaattcggtc atgataataa ttcttagtat
     6781 taattcggca atctgattct actccccccc agtacctgat actacccccc cagtagatta
     6841 atagtgctat gatttttcat attcttgtta accaggtgtt gccatgtctc atacaatccg
     6901 tgataaacag aaactgaaag cgcgtgccag taagattcag ggccaggtcg tggcgctcaa
     6961 gaaaatgctc gacgagccgc acgaatgcgc tgcagtttta caacagattg ctgctatccg
     7021 tggagcggta aacggtctga tgcgggaagt gattaaaggt catctgacgg aacacatcgt
     7081 tcaccagggg gatgagctaa aacgtgaaga agatctggat gtcgttctga aggtgctgga
     7141 ttcatatatc aaataattta ttaacgcgat tgtaaaactg ccgtttttcc tcgtttacaa
     7201 cgggggcgct ggacattacc atcctcctct gcgatttatc atcgcaacca aacgactcgg
     7261 ggtgcccttc tgcgtgaagg ctgagaaata cccgtatcac ctgatctgga taatgccagc
     7321 gtagggaagt cacggaccac caggtcattg cttcttcacg ttatggcagg agcaaactat
     7381 gcaagtcgac ctgctgagtt cagcgcaatc tgcgcacgcg ttacaccttt ttcaccaaca
     7441 ttcccctctt gtgcactgca tgaccaatga tgtggtgcaa acctttaccg ccaatacctt
     7501 gctggcgctc ggtgcatcgc cagcgatggt tatcgaaacc gaagaggcca gtcagtttgc
     7561 ggctatcgcc agtgccttgt tgattaacgt tggcacactg acgcagccac gcgcacaggc
     7621 gatgcgtgct gccgttgagc aagcaaaaag ctctcaaaca cgctggacgc ttgatccagt
     7681 agcggttggt gcgctcgatt atcgccgcca tttttgtcat gaacttttat cttttaaacc
     7741 ggcagcgata cgtggtaatg cttcggaaat catggcatta gctggcattg ctaatggcgg
     7801 acggggagtg gataccactg acgccgcagc taacgcgata cccgctgcac aaacactggc
     7861 acgggaaact ggcgcaatcg tcgtggtcac tggcgaggtg gattatgtta ccgatggaca
     7921 tcgtatcatt ggtattcacg gtggcgatcc gttaatgacc aaagtggtag gaactggctg
     7981 tgcattatcg gcggttgtcg ctgcctgctg tgcgttacca ggcgatacgc tggaaaatgt
     8041 cgcatctgcc tgtcactgga tgaaacaagc cggagaacgc gcagtcgcca gaagcgaggg
     8101 gccaggcagt tttgttccac atttccttga tgcgctctgg caattgacgc aggaggtgca
     8161 ggcatgaaac gaattaacgc tctgacgatt gccggtactg atccgagtgg tggtgcgggg
     8221 attcaggccg atcttaaaac cttctcggca cttggcgctt atggttgctc agttattact
     8281 gcactggtgg cgcaaaatac ccgtggcgta cagtcggtgt atcgcattga gcctgatttt
     8341 gtcgccgccc agctcgattc ggtgttcagc gatgtgcgaa tcgataccac taaaatcggt
     8401 atgttggcgg aaaccgatat tgttgaagcg gtggcagaac ggttgcaacg ttatcagatc
     8461 caaaacgtgg tactcgacac cgttatgctg gcaaaaagcg gcgacccgct gctttcacct
     8521 tcggcggttg ctacgctgcg cagtcgatta ttgccacagg tttcattaat aacgccaaac
     8581 ttgcccgaag ctgccgcctt gctcgacgcg ccacacgcgc gcaccgaaca ggaaatgctg
     8641 gaacaagggc gatcgctgtt ggcgatgggc tgtggcgcag tgctaatgaa aggtggtcat
     8701 ctggatgatg agcaaagccc ggactggctg tttacccgcg agggtgaaca acggtttacc
     8761 gcaccgcgca ttatgaccaa aaacacccac ggcactggtt gtacactctc tgcggcgttg
     8821 gctgcactac gcccgcgcca tacaaactgg gctgacaccg tacaggaggc aaaaatctgg
     8881 ctttcatcgg cgttagccca ggccgacacg ctggaagttg gtcacggtat tggtccggtt
     8941 caccacttcc acgcctggtg gtgagcaccg tactatagct gctttggtta ttgtcagcct
     9001 ttggcactga cattcaggga tgaaaccaat gcagctaaga attaccagtc gaaaaaaact
     9061 caccgctcta ttatgcgctc ttgggctaat ttccatcgtt gcaatttatc cgcgtcagac
     9121 ggtgaatttt ttctattcga cagcagttca gattacagac tacatccact tctacggtta
     9181 tcgcccggtt aaatctttcg ctattcgcat tcctgccagt tacaccattc acggattaga
     9241 tgtttcgcgc tggcaggagc gtatcgactg gcagcgggtg gcaaaaatgc gcgacaacga
     9301 tatccgctta cagtttgctt ttattaaggc tacggaaggc gaaaagctgg tggaccccta
     9361 tttttcgcgt aactggcaat taagccgcga aaatggcctg ctgcgcgggg cgtatcatta
     9421 tttctccccg tcggtatccg cttcagttca ggcgcgatta tttctgcaaa cggtggattt
     9481 ctcacaaggc gatttccctg ccgtgctgga cgtagaagaa cggggaaaat tatcggcaaa
     9541 agaattacgc aagcgggtaa gtcagtggct aaaaatggtc gaaaaacgca cgggaaaaaa
     9601 gccgattatt tactcaggag ccgtttttta tcacaccaat ctggcgggct atttcaatga
     9661 atatccgtgg tgggtggctc actattatca acgtcgtccg gacaatgacg gcatggcctg
     9721 gcgcttctgg cagcattccg accgtggaca ggtagatggc atcaatggtc cggtggattt
     9781 taatgtgttt aatggcacgg ggatgagttg caggcattcg ttgatgggat taaagaaacg
     9841 ccttaaataa gaagcagtga tatactccca ccagactccc tggcaggaaa aagacaaatg
     9901 gaacaagcgc atacccagct gattgcccaa ctgaacgagc gtattttagc ggcggataac
     9961 acgccgcttt atattaagtt tgccgaaacg gtaaaaaatg ccgtgcgcag cggggtgctg
    10021 gagcatggca atattttgcc cggtgagcgt gatttaagtc agttaaccgg cgtttcgcgc
    10081 attacggtgc gcaaggcgat gcaggcgctg gaagaagagg gcgtggtgac gcgttcgcgc
    10141 ggttacggca cgcagatcaa caacatcttc gaatactcgt tgaaagaagc gagaggtttt
    10201 tctcagcagg tggtattgcg cgggaaaaag cccgatacgc tatgggttaa taagcgtgtc
    10261 gtgaaatgtc ccgaagaagt tgcgcagcag ctggcggtcg aagcgggaag tgatgtcttt
    10321 ttgcttaagc gtattcgcta tgtcgatgaa gaagcggtat cgattgagga atcgtgggtt
    10381 ccggcgcatt taattcatga tgttgatgcc atcggaattt ctctttatga ctatttccgc
    10441 agccagcaca tttacccaca acgtacgcgt tcccgcgtta gcgcccggat gccggatgcc
    10501 gagtttcagt cacatattca gttagatagc aaaataccgg tgctggtgat caagcaagtg
    10561 gcgcttgacc aacagcaacg gcctattgag tacagcatta gccactgtcg cagcgatcta
    10621 tacgtttttg tgtgcgagga gtagttcctc gcgcgttggc gcacaatcac cgccccgatg
    10681 cccgacaacc cacgacgcca ctgcattgcc cagtaatacg gcatccgcca gtggcagacc
    10741 agaggccagc ccggcaagta cgccacctgc atgactgtcg cccgccccaa tggtgtctac
    10801 aacttgcgtt ggaaatgccg gaatgcagcc agaagcgtcg ttgctgaaat accatgcgcc
    10861 ttctttatcg agacgaacga tcaacggcgc ggcaaatttc tcctgccatt gcttgccaag
    10921 tgttgttatc tctgcggata aagcaaaacg ttcggcggca atctcagcct cttgacgatt
    10981 gagtgacact aaaggtcgac aggccatgat ccgcgccagt aatgcgtccg ggatatcgcc
    11041 aatacgtggg caaaaatcga taaacggcgt cacgtcttgc agcttttcca gccattccac
    11101 taacaattcg ccgcagggcg aggccagttg ataaccggaa aaatagagca ggttgccagg
    11161 cgcaacggtt aatcgtgcca accattggcg attccattga ttttccacac cgctgaatga
    11221 cataaaagtg cgttcgccat ccggctcaac cagtgccaga caccagccgt tatcgctttc
    11281 ggcgttatcg atcaggctga ttaacccctc tttcgccatc cggttgcgaa taatctccgc
    11341 ccacacgccc tgaccgagcg gcaaggcatt acccgcttcg atgccgaggc gctttaacgc
    11401 cacggcaata ttcagtgcgc agccgccaac gttaacgctc tgctgtttca gttcgatatc
    11461 gcacccacgc caggggaggg cataagcgtc ggcgatcacg tcaatgaccg ccgcgccgac
    11521 gaccatcacc ggctgacgcg tggttaattc aggcagcagt gtgtgtaatc gagcgccgct
    11581 cataccgcct cccgttgttg acgatatttc gccagcgctg tggcatagcg gttgaaatca
    11641 agctgattta ccgcatccag ctccgccttt aacgcaggat cgacagcgtt aatgccatgc
    11701 aacgcgccgc aaatcgccgt cgccatagca cctatggtgt ctgtgtcgcc gccaagattg
    11761 gcgcacagga cggcgcagcg gtttgggtcg gtttgtgcca gttcaactag cgcaatggcg
    11821 cacggaacgg actcaatagt gctggtgcct gcgccaacga cctggtaaag ctgttcgctg
    11881 gcggattcgg tgccgtcggc attacgcaca attttcagcg caatctccag acgtgccgcc
    11941 agtgaggcgc tgaacgtggt ggtgcgtttt tgctgtgcat gtcgcgcaat tgaaggcaat
    12001 gaatcaacaa tcgctgacca gcgttctccg tcaatggcac gagaaatcgc ccatgcgatg
    12061 actaccgcgc ctgcaaccgc cagatcggat ttatgggtcg ggctggacgc cagcgccaca
    12121 tcatcaataa aggaatcaac atcacgcgct ggaagcaaac aacctaatgg cgagacgcgc
    12181 atcgctgcgc cgttggttac gccgttgttt tccagttcag caacgggttt accgtcgcga
    12241 atggcgttaa gcgcaatctt cgaggtcgga cctagtacgt ttttgttaaa ggcgtcgaaa
    12301 cgcaacgccc agtcgagaat attacgccca atcagattcg gatcgatctt gccttcacat
    12361 tccagtaacg catccgccag acacaggcca tcgaggtatc gtcggtgaat tcggcgcggt
    12421 taaaataaca ggccgcgtta ttctcctttg gtccaggcag aaaacggtca atccagccaa
    12481 agtgtgcttt aacgcggctg cgtggccaaa gctcggaggg catccccatc gcatccccta
    12541 acgcctgccc ataaagagca ccgagaatac gttctgtttt catttaactt ccccttgaat
    12601 cggaatttac gggagcatag taatgacaga tattgcgcag ttgcttggca aagacgccga
    12661 caacctttta cagcaccgtt gtatgactat tccttctgac cagctttatc tccccggaca
    12721 tgactacgta gaccgcgtga tgattgacaa taatcgcccg ccagcggtgt tacgtaatat
    12781 gcagacgttg tacaacaccg ggcgtctggc tggcacagga tatctttcta ttctgccggt
    12841 tgaccagggc gttgagcact ctgccggagc ttcatttgct gctaacccgc tctactttga
    12901 cccgaaaaac attgttgaac tggcgatcga agcgggctgt aactgtgtgg catcaactta
    12961 cggcgtgttg gtgtcggtat cgcggcgcta tgcgcatcgc attccatttc tcgtcaaact
    13021 taatcacaac gagacgctaa gttacccgaa tacctacgat caaacgctgt atgccagcgt
    13081 ggagcaggcc ttcaacatgg gcgcggtggc ggttggtgcg actatctatt ttggctcgga
    13141 agagtcacgt cgccagattg aagaaatttc tgcggccttt gaacgcgcgc acgagctggg
    13201 catggtgaca gtgctgtggg cctatttgcg aaactccgcc tttaagaaag atggcgttga
    13261 ttaccatgtt tccgccgacc tgactggtca ggcgaaccat ctggcggcaa ccataggtgc
    13321 agatatcgtc aaacaaaaaa tggcggaaaa taacggcggc tataaagcga ttaattacgg
    13381 ttataccgac gatcgcgtgt acagcaagtt aaccagcgaa aacccgattg atctggtgcg
    13441 ttatcagtta gctaactgct atatgggccg ggctgggttg ataaactccg gcggtgctgc
    13501 aggcggtgaa actgacctca gcgatgcagt gcgtactgcg gttatcaaca aacgcgcagg
    13561 cggaatgggg ctgattcttg gacgtaaggc gttcaagaaa tcgatggctg acggcgtgaa
    13621 actgattaat gccgtgcagg acgtttatct cgatagcaaa attactattg cctgatacgc
    13681 ctcatacgaa cagcaccacc ccgtaggacg gataagacgt ttacactgca tccggcaacc
    13741 tatgcctgat gcgacgcgga agcgtcttat catgcctaca tagcactgcc acgtatgttt
    13801 acaccgcatc cggcataaaa acacgcgcac tttgctacgg cttccctatc gggaggccgt
    13861 ttttttgcct ttcgctcctc gaacaatttt catattgttg tttttgcgat cattatctcg
    13921 acatttaaaa gcaaataatt tcattatatt ttgaaatcga aagcaaacga caagatatga
    13981 aaatgtacgt ggtatcgaca aagcagatgc tgaacaacgc acagcgcggc ggttatgcgg
    14041 ttccggcatt caatattcac aatctcgaaa cgatgcaagt ggtggtggaa accgctgcca
    14101 acctgcatgc gccggtcatc atcgccggaa cgcctggcac atttactcat gctggtacag
    14161 aaaatctgtt ggcgctggtc agcgcgatgg cgaagcaata tcaccatcca ctggcaattc
    14221 atctcgacca tcacacgaaa tttgacgata tcgctcagaa ggttcgttct ggcgtgcgct
    14281 cagtcatgat tgacgcctcg catttgcctt ttgcgcaaaa tatttcacgg gtcaaagagg
    14341 tggtggattt ttgccatcgc tttgatgtca gcgtcgaggc ggagctgggg caacttggcg
    14401 gccaggaaga tgatgtgcaa gtcaatgaag tcgatgcgtt gtacaccaac cccgcacagg
    14461 cgcgtgaatt tgccgaggca accgggattg attccctggc ggtcgccatc ggcacggctc
    14521 acggtatgta tgccagcgca ccggtgcttg atttttctcg actggagaac attcgccagt
    14581 gggtgaactt accgctggtg ctgcatggcg cgtcaggatt atcgactaag gatattcagc
    14641 aaaccatcaa actggggata tgcaaaatca acgttgcaac ggagctgaaa aatgccttct
    14701 tgcagtcgtt aaaaaattac ctgaccgagc accccgaagc gaccgatccc cgggattatt
    14761 tgcagtcggc taaatccgca atgcgcgatg tggtgagcaa agtgattgcc gattgtggct
    14821 gcgagggcag ggcataacgc acctgccatt taacaaggaa aaaacatgaa aacgttaatt
    14881 gcccggcata aagctggtga acatatcggc atatgttcag tctgttctgc ccatccgttg
    14941 gttatcgaag cggcgctggc atttgatcgc aacagcacgc gcaaagtgct gattgaagca
    15001 acgtcaaacc aggtcaatca atttggcggt tataccggaa tgacaccggc agactttcgc
    15061 gaatttgttt ttacgattgc cgataaagtt gggtttgcac gcgaacgcat tattctcggc
    15121 ggcgatcatc tgggaccaaa ctgctggcag caagaaaatg cggatgcggc gatggaaaaa
    15181 tccgtcgagc tggtaaaagc atatgttcgt gctggcttca gtaaaattca tcttgatgcg
    15241 tcaatgtcct gcgcggggga taccataccg ttagcaccag aaacggttgc ggaacgagct
    15301 gctgtgcttt gctttgctgc ggaaagtgtg gcgacagatt gccagcgtga gcaactgagc
    15361 tatgtcattg gcactgaagt tccggttccg ggcggtgagg ccagcgccat tcagtcagta
    15421 cacatcaccc atgttgaaga tgccgccaat actttacgta cgcatcaaaa ggcctttatt
    15481 gcccgtgggc tgacagaggc gttaacacgt gtgattgcca tcgtggtgca gccgggtgtg
    15541 gaatttgatc acagcaatat tatccattat cagccgcagg aagcgcaggc gctggcgcaa
    15601 tggatagaaa acacccgaat ggtttatgaa gcacattcta ccgattacca gacccggacg
    15661 gcttattggg aattagtccg cgatcacttt gcaatattga aagtcggtca cgcattaacc
    15721 tttgctttac gcgaggcgat atttgcactg gcacaaattg agcaggaact tatcgccccc
    15781 gaaaatcgca gcggttgcct ggcggtaatt gaagaagtga tgctcgacga accgcaatac
    15841 tggaaaaaat attatcgcac gggttttaac gattcattac tggatattcg ttacagcctg
    15901 tcggatcgta ttcgttatta ctggccgcat agtcggatta aaaatagcgt tgaaacgatg
    15961 atggtgaatc tgcaaggcgt ggacatccca ctgggcatga ttagtcagta tcttcccaaa
    16021 caatttgaac gcattcagtc cggggaatta tcagcaatgc cgcatcagct tattatgaat
    16081 aaaatttatg atgttttgcg cgcctatcgc tacggctgtg cggaataagg acggtatatg
    16141 actaacctgt ttgttcgtag cggaatttct tttgtcgatc gtagcgaagt tttaacctat
    16201 atcggtaatg agatgctcgc gaaaggtgtg gttcatgata cctggccaca ggcattaatt
    16261 gccagagaag cagaattccc taccgggata atgcttgagc agcacgccat tgcaataccg
    16321 cattgtgagg cgattcatgc taagtcgtca gccatttatc tgttaaggcc aacaaataaa
    16381 gttcattttc agcaagcgga tgatgataac gacgtggcgg tatcgttggt tattgcgttg
    16441 attgtggaaa atccgcagca gcaattgaaa cttttacgct gtttatttgg caagttacaa
    16501 cagcccgata tcgtcgagac actaattact cttcctgaaa ctcagttaaa ggaatacttc
    16561 acaaagtatg ttttagattc agacgaataa atccctctgt aacaataaaa aggactattt
    16621 atgaaacgca agattattgt cgcttgcgga ggcgcggttg cgacctctac gatggcggcg
    16681 gaagaaatta aagagttgtg tcagagtcat aatattcctg ttgaattaat ccagtgtcgg
    16741 gttaatgaaa tagaaaccta tatggatggt gtgcatttga tatgtaccac tgccagggtg
    16801 gatcgtagtt ttggcgatat tccgttagtt cacggcatgc cttttgtttc tggtgtcggt
    16861 atcgaagcat tacaaaataa aattctgact atcttacagg gatgacctat gttttcagaa
    16921 gtcatgcgtt atattctcga cctcggccct acagtgatgc tgccggttgt catcattatt
    16981 ttttctaaaa tattaggcat gaaggcaggc gattgcttta aagcgggtct gcatatcggg
    17041 attggctttg ttggcattgg ccttgtgatt ggcttaatgc tggattccat tggtccggcg
    17101 gcgaaagcga tggcggaaaa tttcgacctg aatctgcatg tggtcgatgt cggctggccg
    17161 ggctcttcac caatgacctg ggcgtcgcaa attgcgctgg tggcgattcc gattgcgata
    17221 ttggttaacg tggcgatgtt actgacccgt atgacgcggg tggtaaatgt tgatatctgg
    17281 aatatctggc atatgacctt caccggcgcg ttgctgcacc tggcaaccgg ttcatggatg
    17341 atagggatgg cgggtgtggt aattcacgcg gcgtttgttt ataagctcgg cgactggttt
    17401 gcccgcgata cccgaaattt ctttgagctg gaaggtattg ctattccgca cggtacgtcg
    17461 gcgtatatgg ggccgattgc ggtgctggtc gatgctatca tcgagaaaat cccaggcgtt
    17521 aaccgaatta aatttagcgc cgacgatatt cagcgcaaat ttggcccttt tggcgaacct
    17581 gtcaccgtgg gttttgtgat ggggctgatt atcggcatcc tcgcgggtta cgatgtcaaa
    17641 ggtgtattgc agctggcggt aaaaacggcg gcagttatgc tgctgatgcc acggatgatt
    17701 aaacccatca tggatggttt aacgcccatc gctaagcagg cccgtagtcg tttacaggcg
    17761 aagttcggcg gtcaggagtt cctgattggc ctggatcccg cattactgct ggggcatacg
    17821 gcggtggtat cggcaagcct gatttttatt ccgctcacca ttttaattgc tgtttgtgtt
    17881 ccgggtaatc aggtactgcc gtttggcgat cttgccacta tcggcttctt cgtggcgatg
    17941 gcggtcgcgg tgcatcgtgg aaatctgttc cgcaccttaa tctcgggtgt catcattatg
    18001 agcatcaccc tgtggatcgc gacgcaaact attggtttgc acacccaacc ggcggctaat
    18061 gctggggcgt taaaagccgg gggtatggtg gcttcaatgg atcagggcgg ttctcccatt
    18121 acctggttac tgattcaggt tttctccccg caaaatattc ccggtttcat tattatcggc
    18181 gcaatttatc tgaccggtat tttcatgacc tggcgtagag cgcgtggttt tactaaacaa
    18241 gagaaagccg ttctcgcaga ataattttta cctgaggggg aggttatccc ccttaacaat
    18301 cagggtaatg ctgccaactt actgatttag tgtatgatgg tgattttaag gtgcttgcgt
    18361 ggcttccatt tccatcagat gtccttcctg ctccgctact gaaggcgtgg tgcgtaacgg
    18421 caaaagcact gccggacatc agcgctatct ctgctctcat tgccgtaaaa catggcaact
    18481 acagtt
//
LOCUS       DAETQL010000077        18450 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.077, whole genome shotgun sequence.
ACCESSION   DAETQL010000077 DAETQL010000000
VERSION     DAETQL010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.077"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..1238
                     /locus_tag="K0W65_002965"
                     /pseudo
     CDS             1..1238
                     /locus_tag="K0W65_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
     gene            1388..1819
                     /gene="ndk"
                     /locus_tag="K0W65_002966"
     CDS             1388..1819
                     /gene="ndk"
                     /locus_tag="K0W65_002966"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBI3096149.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            1969..3123
                     /gene="trmG/rlmN"
                     /locus_tag="K0W65_002967"
     CDS             1969..3123
                     /gene="trmG/rlmN"
                     /locus_tag="K0W65_002967"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBI3096150.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            3408..4421
                     /gene="rodZ"
                     /locus_tag="K0W65_002968"
     CDS             3408..4421
                     /gene="rodZ"
                     /locus_tag="K0W65_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBI3096151.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            4448..5575
                     /gene="ispG"
                     /locus_tag="K0W65_002969"
                     /gene_synonym="gcpE"
     CDS             4448..5575
                     /gene="ispG"
                     /locus_tag="K0W65_002969"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBI3096152.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            5678..6952
                     /gene="hisS"
                     /locus_tag="K0W65_002970"
     CDS             5678..6952
                     /gene="hisS"
                     /locus_tag="K0W65_002970"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBI3096153.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            6970..7590
                     /gene="yfgM"
                     /locus_tag="K0W65_002971"
     CDS             6970..7590
                     /gene="yfgM"
                     /locus_tag="K0W65_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HBI3096154.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            7601..8779
                     /gene="bamB"
                     /locus_tag="K0W65_002972"
     CDS             7601..8779
                     /gene="bamB"
                     /locus_tag="K0W65_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBI3096155.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            8897..10369
                     /gene="der"
                     /locus_tag="K0W65_002973"
     CDS             8897..10369
                     /gene="der"
                     /locus_tag="K0W65_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBI3096156.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            10439..10654
                     /locus_tag="K0W65_002974"
     CDS             10439..10654
                     /locus_tag="K0W65_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBI3096157.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(10651..12021)
                     /gene="xseA"
                     /locus_tag="K0W65_002975"
     CDS             complement(10651..12021)
                     /gene="xseA"
                     /locus_tag="K0W65_002975"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBI3096158.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            12183..13649
                     /gene="guaB"
                     /locus_tag="K0W65_002976"
     CDS             12183..13649
                     /gene="guaB"
                     /locus_tag="K0W65_002976"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBI3096159.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            13718..15295
                     /gene="guaA"
                     /locus_tag="K0W65_002977"
     CDS             13718..15295
                     /gene="guaA"
                     /locus_tag="K0W65_002977"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBI3096160.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(15390..15929)
                     /locus_tag="K0W65_002978"
     CDS             complement(15390..15929)
                     /locus_tag="K0W65_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HBI3096161.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(15945..16463)
                     /locus_tag="K0W65_002979"
     CDS             complement(15945..16463)
                     /locus_tag="K0W65_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBI3096162.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(16774..16965)
                     /locus_tag="K0W65_002980"
     CDS             complement(16774..16965)
                     /locus_tag="K0W65_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBI3096163.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            16983..17135
                     /locus_tag="K0W65_002981"
     CDS             16983..17135
                     /locus_tag="K0W65_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096164.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            17317..>18450
                     /gene="pdeF"
                     /locus_tag="K0W65_002982"
     CDS             17317..>18450
                     /gene="pdeF"
                     /locus_tag="K0W65_002982"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBI3096165.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4361 a         4481 c         4992 g         4616 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgtc gccccactgg catggaaaac gggcaccagc tatggctatc
      481 gtgacgcctg ggcgattggg gttaacgctc gctatgtcat tgggatctgg actggcagac
      541 ctgacggcac gcccgttgtt ggtcagtttg gctttgccag tgccgtacca ttgttgaatc
      601 aggtcaataa tatcttactg tcgcgcagtg cgaatctgcc ggaagacccg cgcccgaact
      661 cagtcactcg tggcgttatc tgctggccgg gcggacagtc tttgccggaa ggtgacggta
      721 actgtcgccg ccgcctggca acctggctgc tggatggcag ccagccgcca acgctgttac
      781 tgtcagagca ggaagggatt aatggcattc gcttccccat ctggctggat gacaaagggc
      841 agcgcgtcgc cgctgactgc ccgcaggcga agcaagaaat ggtaaatgtc tggccgctac
      901 cgctggaacc ctggctgccc gcatcagaac gtcgcgctgt gcgcttacca ccggcctcga
      961 caatttgccc gccatacggt cacgatgccc agctccctct gcagctgaca ggcgttcgcg
     1021 atggcgcgat tattaaacgt ttaccgggcg cagcggaagc gactttgccg ttgcaatcaa
     1081 gtggaggggc aggtgaacgc tggtggtttc tgaatggcga accgttaact gaacgcgggc
     1141 gcaacgtgac cctgcatttg acggataaag gtgattatca attgctggtg atggatgatg
     1201 tggggcaaat cgcgacagtg aaatttgtca tgcaatagtc tgttttgtcc ctgattgttg
     1261 ctaaaactca ttttatttta aaaaaaatgt taccttcatc aatagtcaac ggccctgttg
     1321 ctcattataa tccgcgccat ctcgtacgct ggtacagaca acaacagaac aatttacaga
     1381 ggtaaaaatg gctattgaac gtactttttc catcatcaaa ccgaacgcgg tagcaaaaaa
     1441 cgtcattggt aatatctttg cgcgctttga agctgcaggg ttcaaaattg tcggcaccaa
     1501 aatgctgcat ctgaccgttg aacaggcacg tggcttttat gctgaacacg atggaaaacc
     1561 gttctttgat ggtctggttg aattcatgac ctctggcccg atcgtggttt ccgtgctgga
     1621 aggtgaaaac gccgttcagc gtcaccgcga tctgctgggc gcgaccaatc cggcaaacgc
     1681 actggctggt actctgcgcg ctgattacgc tgacagcctg accgaaaacg gtacccacgg
     1741 ttctgattcc gtcgaatctg ccgctcgcga aatcgcttat ttcttcggcg aaggcgaagt
     1801 gtgcccgcgc acccgttaat aatttcgtaa atgccgcgtg caaacgtggc atccgtgcgc
     1861 cagaatttgt acaatgcagc gcccccggac gagcagccgc tcaccggggc gtttcttttt
     1921 tcaaccctcc aggggccata acgtgtaata acgaggccgg aatagcatat gtctgaacaa
     1981 ttagtcacac ctgaaaacgt caccacgaaa gatggaaaaa tcaacctgct ggatctcaac
     2041 cgtcagcaga tgcgggagtt ttttaaagat ttaggtgaaa aaaccttccg cgccgatcag
     2101 gtgatgaagt ggatgtatca ctattgctgc gacaactttg atgagatgac cgacatcaac
     2161 aaagtgttgc gcggcaaact gaaagaggtg gcggaaatcc gcgcaccgga agtggttgaa
     2221 gaacagcgtt catccgacgg caccattaaa tgggcgatcg ccgttggcga tcagcgcgtc
     2281 gaaacggtgt atatcccgga agacgaccgt gccacgctct gcgtctcttc gcaggtgggg
     2341 tgtgcgctgg agtgtaaatt ctgttccacc gcccagcagg gctttaaccg caacctgcgg
     2401 gtatcggaaa ttattggcca ggtgtggcgt gcggcgaaaa tcgtcggcgc agcaaaagtc
     2461 accggtcagc gcccaatcac taacgtcgtg atgatgggca tgggcgagcc gctgctcaac
     2521 ctgaacaacg tcgttccggc gatggaaatc atgcttgatg atttcggttt tggcctgtct
     2581 aaacgtcgcg tgacgctttc cacttccggt gtagttccgg cgctggataa actgggcgat
     2641 atgatcgacg ttgcactggc aatttccctg cacgcgccga acgacgaaat tcgtgacgaa
     2701 attgtgccga tcaacaaaaa gtacaatatc gaaacgttcc ttgctgcggt gcgccgttat
     2761 ctggagaaat ccaacgccaa tcagggccga gtcactattg aatacgtgat gcttgaccac
     2821 gttaacgacg gcactgaaca cgcgcaccaa ctggcggaac tgctgaaaga tacgccgtgt
     2881 aagatcaacc tgatcccgtg gaacccgttc ccggacgcgc cgtatggacg cagttcgaac
     2941 agccgtatcg accgtttctc aaaagtgctg atgagctacg gtttcaccac tattgtgcgt
     3001 aaaacgcgtg gtgatgatat cgatgctgcc tgtggtcagt tggcgggcga tgttatcgac
     3061 cgtacgaaac gtactctgcg taaacggatg cagggtgaag ccatcgatat taaagcggtc
     3121 tgatagccat tacgccacgg ttactttatg taccgtggcg taatctttta tgaatgaagt
     3181 attaagttgc aattgtctgg tcaatattaa cggtgcgttt ttgctgactt taaggcagta
     3241 tgtaacgatg caacagtaag ttagcctcag tgaatgtggg ctttgtcacg agcacacaga
     3301 cggtcttata ctgtatgata acggttaact taacggatgt ttcgcggtgt gggtgagcat
     3361 gatggttcac cggcatctca attctcattt aaacgtacct gcagcgaatg aatactgaag
     3421 ccacgcacga ccaaaatgaa gcacttacta ccggcgctcg cctgcgtaat gctcgcgaac
     3481 aactaggact tagtcagcag gccgttgccg agcgactttg cctgaaggtt tccacggtac
     3541 gcgacattga agaagataag gcacccgccg atcttgcttc aacattcctg cgcggatata
     3601 tccgctctta tgcgcgtctg gtacatattc cagaagaaga actgctgcca gggctggaaa
     3661 agcaggctcc acttcgggct gcaaaagttg cgccaatgca gagtttttcc ctcggtaaac
     3721 gccgcaaaaa acgcgacggc tggctaatga ccttcacctg gctggtgttg tttgtggtta
     3781 tcggcctgag cggtgcctgg tggtggcaag accacaaagc tcagcaggaa gagatcacca
     3841 ctatggccga tcaatcttcg gcggaactga gcagtaatag cgagcagggg cagagtgttc
     3901 cgttaaatac gtcgacaact acagacccgg ctacaacgtc cacgccgcca gcgtctgtgg
     3961 atactaccgc aaccaacacg caaacacctg ccgtaactgc gccagcacca gctgttgatc
     4021 cgcaacagaa tgcggttgtt tcgccttcgc aggcaaatgt tgataccgcc gcgaccccgg
     4081 taccaacggc agcaacaacg ccagatggtg ctgcgccgtt gccaaccgat caggctggcg
     4141 tgaccacgcc ggcggctgat ccgaatgcgc tggtgatgaa ctttactgcc gattgctggc
     4201 tggaggtcac tgatgctacc ggtaaaaaat tgtttagcgg gatgcagcgt aaagacggta
     4261 atttgaattt aaccggccag gcaccgtata aactgaaaat tggtgcgcca gccgcagtac
     4321 agatccagta tcaagggaaa cctgtcgatc tgagtcgttt tatcagaact aaccaggttg
     4381 cgcgtctgac cctcaatgcc gaacaatcac cggcgcagta acagacgggt aacgcgggag
     4441 atttttcatg cataaccagg ctccaattca acgtagaaaa tcaacacgta tttacgttgg
     4501 gaatgtgccg attggcgatg gtgctcccat cgccgtacag tccatgacca atacgcgtac
     4561 gacagatgtc gaagcaacgg tcaatcaaat caaggcgctg gaacgcgttg gcgctgatat
     4621 cgtccgtgtc tccgtaccga cgatggacgc ggcagaagcg ttcaaactca tcaaacagcg
     4681 ggttaacgtg ccgctggtgg ctgacatcca cttcgactat cgcattgcgc tgaaagtagc
     4741 ggaatacggc gtcgattgtc tgcgtattaa ccctggtaat atcggtaatg aagagcgtat
     4801 tcgcatggtg gttgactgtg cgcgcgataa aaacattccg atccgtattg gcgttaacgc
     4861 cggatcgctg gaaaaagatc tgcaagaaaa gtatggcgaa ccgacgccgc aggcgttgct
     4921 ggaatccgcc atgcgtcatg ttgatcatct cgatcgcctg aacttcgatc agttcaaagt
     4981 cagcgtgaaa gcgtctgacg tcttcctcgc tgttgagtct tatcgtttgc tggcaaaaca
     5041 gatcgatcag ccgctgcatc tggggatcac cgaagccggt ggtgcacgca gcggggcagt
     5101 aaaatccgcc attggtttag gtctgctgct gtcggaaggc atcggcgaca cgctgcgtgt
     5161 atcgctggcg gccgatccgg tcgaagagat caaagtcggt ttcgatattt tgaaatcgct
     5221 gcgtatccgt tcgcgaggga ttaacttcat cgcctgcccg acctgttcgc gtcaggaatt
     5281 tgatgttatc ggtacggtta acgcgctgga gcaacgcctg gaagatatca tcactccgat
     5341 ggacgtttcg attatcggct gcgtggtgaa tggcccaggt gaggcgctgg tttctacact
     5401 cggcgtcacc ggcggcaaca agaaaagcgg cctctatgaa gatggcgtgc gcaaagaccg
     5461 tctggacaac aacgatatga tcgaccagct ggaagcacgc attcgtgcga aagccagtca
     5521 gctggacgaa gcgcgtcgaa ttgacgttca gcaggttgga aaaataataa cgtgatggga
     5581 agcgcctcgc ttcccgtgta tgattgaacc cgcatggctc ccgaaacatt gagggaagcg
     5641 ttgagggttc atttttatat tcagaaagag aataaacgtg gcaaaaaaca ttcaagccat
     5701 tcgcggcatg aacgattacc tgcctggcga aacggccatc tggcagcgca ttgaaggcac
     5761 actgaaaaac gtgctcggca gctacggtta cagtgaaatc cgcttgccga ttgtagagca
     5821 gaccccgcta ttcaaacgtg cgattggtga agtcaccgac gtggttgaaa aagagatgta
     5881 cacctttgag gatcgcaatg gcgacagcct gactctgcgc cctgaaggga cggcgggctg
     5941 tgtacgcgcc ggcatcgagc atggtcttct gtacaatcag gaacagcgtc tgtggtatat
     6001 cgggccgatg ttccgtcacg agcgtccgca gaaagggcgt tatcgtcagt tccatcagtt
     6061 gggctgcgaa gttttcggtc tgcaaggtcc ggatatcgac gctgaactga ttatgctcac
     6121 tgcccgctgg tggcgtgcgc tgggtatctt cgaacacgta actcttgagc tgaattctat
     6181 cggttcgctg gaagcacgcg ccaattaccg cgatgcgctg gtggcattcc ttgagcagca
     6241 taaagaaaag ctggacgaag actgcaaacg ccgcatgtac actaacccgc tgcgcgtgct
     6301 ggattcaaaa aatccggaag tgcaggcgct tctcaacgac gctccggcat taggtgatta
     6361 tctggacgag gaatctcgtg agcattttgc cggtctgtgc aaactgctgg agagcgcggg
     6421 gatcgcttac accgtaaacc agcgtctggt gcgtggtctg gattactata accgtaccgt
     6481 tttcgagtgg gtgactaaca gtctcggctc ccagggcacc gtgtgtgcag gcggtcgtta
     6541 tgacggtctt gtggaacaac tgggcggtcg tgcaacaccg gctgtcggtt ttgcgatggg
     6601 cctcgaacgt cttgtattgt tagtacaggc cgttaatccg gaatttaaag ccgatcctgt
     6661 tgtcgatata tacctggtgg cttcaggtgc tgatacacaa tctgcggcta tggcattagc
     6721 tgagcgtctg cgtgatgaat tactgggcgt gaaattgatg accaaccacg gcggcggcaa
     6781 ctttaagaaa cagtttgccc gtgctgataa atggggtgcc cgcgttgctg tggtgctggg
     6841 tgagtctgaa gtggctaacg gcacagcagt agtgaaggat ttgcgctctg gtgagcaaac
     6901 ggcagttgcg caggatagcg tagccgcgca tttgcgcacg ttactgggtt aaggaaggag
     6961 aaggacagcg tggaaattta cgagaacgaa aacgaccagg tagaagcggt taaacgcttt
     7021 tttgctgaaa atggcaaagc actggctgtt ggggggattt tgggcgttgg cgcactgact
     7081 ggctggcgct actggaacag ccatcaggtt gattctgcac gctccgcttc tcttgcctat
     7141 caaaatgcgg ttaccgcagt gagcgaaggc aaaccggata gcatcccggc ggcggaaaaa
     7201 tttgctgctg aaaataaaaa tacttatggt gcgctggctt ctttggaact tgcgcagcaa
     7261 tttgttgaca aaaatgaact agagaaagct gccgcccagt tacaacaggg gctggcagac
     7321 acgagcgatg aaaatctcaa agccgtgata aatctgcgtc ttgctcgcgt tcaggtacag
     7381 ctcaagcagg ctgatgccgc gctgaaaacc cttgatacca tcaaaggtga agggtgggct
     7441 gccattgttg ccgacttgcg tggtgaagca ttgctgagca aaggtgataa gcaaggtgcg
     7501 cgtagtgcat gggaagcagg cgtgaaaagc gatgttactc cggcactgag cgaaatgatg
     7561 cagatgaaaa ttaataattt gtccatctga gagggacccg atgcaattgc gtaaattact
     7621 gctgccagga ctgctttccg ttaccctttt aagcggctgt tcgctgttta acagcgaaga
     7681 agatgtggta aagatgtccc cattgccaac cgttgaaaac cagtttacgc cgaccacggc
     7741 gtggagcact tccgttggta gcggcattgg caacttctat tccaatcttc atccggcact
     7801 ggcggacaac gttgtctatg cagcggaccg cgctggtttg gtaaaagcgc tgaatgcgga
     7861 tgatggcaaa gaaatctggt ctgtcagcct ggccgagaaa gatggctggt tctctaaaga
     7921 gcctgcatta ctttctggcg gtgtgaccgt gtctggtggg catgtctaca ttggcagcga
     7981 aaaggcgcag gtttacgcgc tgaataccag cgatggtact gtggcatggc aaactaaagt
     8041 cgcgggtgaa gcactttcgc gcccggtggt cagcgacggt ctggtgttaa tccacaccag
     8101 taacggtcag ttacaagcgc tgaacgaagc tgacggcgct gtcaaatgga cagttaacct
     8161 cgatatgcct tcgctctctt tgcgtggcga gtctgcgccg gcaacggctt ttggtgcggc
     8221 cgtcgtgggt ggcgataatg gtcgcgtcag cgcagtgctg atggaacagg gccagatgat
     8281 ttggcagcag cgtatttccc aggcgaccgg ttctaccgaa attgaccgtc tgagcgatgt
     8341 tgacacgact cccgtcgttg ttaacggcgt tgttttcgcg ctggcctata atggtaacct
     8401 gacggcgctt gatctgcgca gtggtcagat tatgtggaaa cgcgaactgg gttcggtgaa
     8461 tgatttcatc gtcgatggca atcgcatcta tctggtcgat caaaatgacc gggtgatggc
     8521 gttgaccatt gatggcggcg ttacgctgtg ggcacaaagc gatctgctgc atcgcctgct
     8581 gacttctccg gtgctgtata atggcaacct ggtggtcggt gacagtgaag gttatctgca
     8641 ctggattaac gtcgaagatg gtcgtttcgt tgcccagcaa aaagttgata gttccggttt
     8701 ccagactgaa ccggttgccg ctgacggcaa actgctgatc caggcaaaag acggaaccgt
     8761 gtactctatt acacgttaat cgtctctgtc gttcactttg aaaacggctc ctggacaggg
     8821 gccgttttcc tgtttttaac aacgacgcga atatagcgtg cgttgtctga tgatttataa
     8881 aaatgaggct ttaaacatgg tacctgtggt cgcgcttgtc gggcgcccta acgtaggaaa
     8941 atccacgtta tttaaccgtc taactcgcac ccgagatgcg ctggttgcgg atttcccggg
     9001 tctgactcgt gaccgtaagt acggtcgtgc ggaaattgaa ggccgtgagt ttatctgtat
     9061 tgataccggc gggattgatg gcacagaaga cggtgtagaa acccgcatgg cggaacagtc
     9121 gctgctggcg attgaagaag cggacgtcgt actgtttatg gtggatgcgc gcgcgggcct
     9181 gatgccggca gatgaagcga ttgccaaaca tctgcgctcc cgtgaaaaac cgaccttcct
     9241 ggtggtaaac aaaactgacg gtctggatcc cgatcaggca gtggttgatt tctactcgct
     9301 tggtttaggt gaaatctacc cgatcgccgc gtctcacggt cgtggcgtat taagtctgct
     9361 ggagcatgtg ctgctgccgt ggatggaaga tctcgcaccg caagaggaag tcgacgaaga
     9421 cgctgaatac tgggcgcaat ttgaagcgga agagaacggc gaagaagaag aggaagacga
     9481 cttcgacccg caaagtctgc cgatcaaact ggcgattgtg ggtcgtccga acgtaggtaa
     9541 gtctacactc actaaccgta ttcttggaga agagcgcgtt gttgtttacg acatgcctgg
     9601 cacgacgcgt gacagcatct acatcccaat ggaacgcgat ggacgtgagt atgtgctcat
     9661 tgacaccgct ggcgtacgta aacgcggcaa aatcaccgat gctgtagaga aattctccgt
     9721 aatcaaaacg ttgcaggcca ttgaagacgc caacgtggtg atgttagtga ttgatgcgcg
     9781 cgaaggtatt tccgatcagg atctctcgct gctgggcttt attctcaata gtgggcgctc
     9841 acttgtcatt gtggtgaata agtgggatgg cctgagccag gaagtgaaag agcaggtgaa
     9901 agaaacgctg gacttccgtc tgggctttat cgattttgct cgtgtgcact ttatctctgc
     9961 cttgcacggc agtggtgttg gtaacttgtt tgaatcagta cgtgaagcgt atgacagctc
    10021 cacccgtcgt gtggggacct ctatgctgac gcgcatcatg acgatggctg ttgaagatca
    10081 ccaaccgccg ctggtacgcg gtcgtcgtgt gaagctgaaa tatgcccacg ctggtggtta
    10141 taacccgccg attgtggtga ttcacggtaa tcaggtgaaa gacctgcctg attcctacaa
    10201 gcgctacttg atgaactact tccgcaaatc gctggacgta atgggatcgc cgattcgtat
    10261 tcagttcaaa gaaggggaaa acccgtatgc gaacaagcgt aacaccctga cgccaaccca
    10321 gatgcgtaaa cgtaagcgtc tgatgaagca catcaagaaa aataaataat tatccgtaag
    10381 tattttatct gccattccct ctatgaatgt agagggaatg gctaccggag gaattcatat
    10441 ggaactgcat tgtccaaaat gtcagcatgt gcttgatcag gataacggcc atgcgcgttg
    10501 cccatcctgc ggggaattta ttgaaatgaa agctctttgt ccagactgcc atcaaccgtt
    10561 gcaggtgtta aaagcctgcg gtgcagtaga ttacttctgt cagcacgggc atggcttgat
    10621 atcgaaaaaa cgcgttgaat tcgtgctggc ttaatgaacc tttttacgcg atttttttac
    10681 cggctggatg ttttttactt cactttctat ccagccgtct tccagacgtg tggttagcat
    10741 ttcacccgtt ttaacttgct tcactttttt cagtacattg ccgtcagtag cagtagtaac
    10801 gctgtatcca cgcgccagcg ttgacagtgg gcttacggct tcgaggtgcg ttactgcatt
    10861 accgaaacgt tcacgcgtgg cgctaagctg tgcgcgcagg atttctgcta aacgatattc
    10921 cagttgctga atgcgcgttt gcgtgcgatg aatcttcggc tgtggatttt gctgattcag
    10981 ccgctgtgtt aatcgctgct gctgttgccc ggcacgctta agctgatttt ccagcgcaaa
    11041 gctcatccgt ttttgcaggc gttcaagcat ggtttgctgg cgtgccagcc ggagctgcgg
    11101 atgctgctgc tgcaagcgat gatgaatctg cgtaaagcga cgtgtacggt tggcgagata
    11161 ataatccatc gccatctcca gccgttgacg ggccgattgc acctggcgca gtaactcttg
    11221 ctgattacgg ctcactactt cagcggcggc agacggcgtt ggcgcacgca gatcggcaac
    11281 aaaatcggca atggtcacat ccgtctcatg cccgacggcg ctgacgaccg gaatgcggct
    11341 ggcaaaaatc gcccgcgcta cgcgttcgtc gttaaaactc cataaatctt ccagcgaacc
    11401 gccgccgcgc ccaacgatca acacgtcgca ctcattgcgc tgattcgcca gttcaatggc
    11461 gcgaacaatt tgccccggcg cgtcatcgcc ctgaacggag gtggggtaga tgatcaccgg
    11521 tagagaagga tcgcgacgtt ttaacacatg caaaatatca tgtagcgcag caccggtttt
    11581 tgaggtgatc acaccaacgc aatgcgcagg ggagggaagt ggttttttgt attgctgatc
    11641 gaacaaacat tcagcctgca acttcgcttt gagctgttcg tacttctgtt gcagcagccc
    11701 ttcaccggcc ggctgcatac tctcaacgat tatctggtag tcgccgcgcg gctcgtagag
    11761 cgtaatattg gcgcgaacta aaacttgttg cccatgctgc ggacggaagg tgacccggcg
    11821 gttgctgttg cggaacatcg cgcagcgtac ctgggcggtg tcgtctttga gtgtaaagta
    11881 ccagtgaccg gaagccggtt gtgtgaaatt agagatttcg ccgctgatcc aaacctgtcc
    11941 catctcatgc tcaagcagca gacgaaccgt ttgattcagg cgactaacgg taaaaattgc
    12001 aggggattga gaaggtaaca tgtgagcgag atcaaattct aaatcagcag gttattcagt
    12061 ctatagtaac ccgcccttcg gggatagcaa gcattttttg caaaaagggg tagatgcaat
    12121 cggttacgct ctgtataatg ccgcggcaat atttattaac cactctggtc gagatattgc
    12181 ccatgctacg tatcgctaaa gaagctctga cgtttgacga cgttctcctc gttcctgctc
    12241 attctaccgt tctgccgaat actgctgacc tcagcaccca gctgacgaaa actattcgtc
    12301 tgaatatccc tatgctttcc gcagcaatgg ataccgtaac ggaagcgcgc ctggctattg
    12361 ctctggctca ggaaggcggt atcggcttta tccacaaaaa catgtccatt gaacgccagg
    12421 cagaagaagt tcgccgtgtg aaaaaacacg aatctggtgt ggtgactgat ccgcagactg
    12481 ttctgccaac cacgacgctg cgcgaagtga aagaactgac cgagcgtaac ggttttgcgg
    12541 gctatccggt cgttaccgaa gaaaacgaac tggtgggtat tatcaccggt cgtgacgtgc
    12601 gttttgttac cgacctgaac cagccggtta gcgtttacat gacgccgaaa gagcgtctgg
    12661 tcaccgtgcg tgaaggtgaa gcccgtgaag tggtgctggc aaaaatgcac gaaaaacgcg
    12721 ttgaaaaagc gctggtggtt gatgacgaat tccacctgat cggcatgatc accgtgaaag
    12781 acttccagaa agcggaacgt aaaccgaacg cttgtaaaga cgagcaaggc cgtctgcgtg
    12841 ttggtgctgc agttggcgca ggtgcgggta acgaagagcg tgttgacgcg ctggttgccg
    12901 ctggcgttga cgttctgctg atcgactcct cccacggtca ctcagaaggc gttttgcagc
    12961 gtatccgtga aacccgtgct aaatatccgg atctgcaaat catcggcggc aacgtggcaa
    13021 cagctgcagg tgcacgcgct ttggcagaag ctggttgcag cgcggttaaa gtcggtatcg
    13081 gccctggttc tatctgtacg actcgtatcg ttactggcgt aggtgttccg cagatcactg
    13141 ccgttgctga cgcagttgaa gccctggaag gcaccggtat tccggttatc gcagatggtg
    13201 gtattcgctt ctccggcgac atcgccaaag ctatcgccgc tggcgcaagc gcggtgatgg
    13261 taggttccat gctggcgggt actgaagaat ctccgggtga aatcgaactc taccagggcc
    13321 gttcttataa atcttaccgt ggtatgggtt ccctgggcgc gatgtccaaa ggttcctctg
    13381 accgttattt ccagagcgat aacgctgccg acaaactggt gccggaaggt atcgaaggtc
    13441 gcgtagctta taaaggtcgc ctgaaagaga tcattcacca gcagatgggc ggcctgcgct
    13501 cctgtatggg tctgaccggc tgtggtacta tcgacgaact gcgtactaaa gcggagtttg
    13561 tacgtatcag cggtgcgggc attcaggaaa gccacgttca cgacgtgacc attactaaag
    13621 agtccccgaa ctaccgtctg ggctcctgat tctcttcgcc cgatttcatg tcgggcgatt
    13681 tatattatct gtttcacttg cctcggaata agcgtcaatg acggaaaaca ttcataagca
    13741 tcgcatcctc attctggact tcggttctca gtacactcaa ctggttgcgc gccgcgtgcg
    13801 tgagctgggt gtttactgcg aactgtgggc gtgggatgtg acagaagcac aaattcgtga
    13861 cttcaatcca agcggaatta ttctttccgg cggcccggaa agtactactg aagaaaacag
    13921 tccgcgtgcg ccgcagtatg tctttgaagc aggcgtaccg atattcggcg tttgctatgg
    13981 catgcagacc atggcaatgc agttgggcgg tcacgttgaa gcctctaacg aacgtgaatt
    14041 tggctacgcg caggtagaag tcgtaaacga cagcgcactg gttcgcggta tcgaagatgc
    14101 gctgaccgca gacggtaaac cgctgctcga tgtctggatg agccacggcg ataaagttac
    14161 cgctatcccg tccgacttcg tcaccgtagc cagcaccgaa agctgcccgt ttgccattat
    14221 ggctaacgaa gaaaaacgct tctatggcgt acagttccac ccggaagtga ctcacacccg
    14281 ccagggtatg cgcatgctgg agcgttttgt gcgtgatatc tgccagtgtg aagccctgtg
    14341 gacgccagcg aaaattatcg acgatgctgt agctcgcatc cgcgagcagg taggcgacga
    14401 taaagtcatc ctcggcctct ctggtggtgt ggattcctcc gtaaccgcaa tgctgctgca
    14461 ccgtgctatc ggtaaaaacc tgacttgcgt attcgtcgac aacggcctgc tgcgtctcaa
    14521 cgaagcagag caggttctgg atatgtttgg cgatcacttt ggtcttaaca ttgttcacgt
    14581 accggcagaa gatcgcttcc tgtcagcgct ggctggcgaa aacgatccgg aagcaaaacg
    14641 taaaatcatc gggcgcgttt tcgttgaagt attcgatgaa gaagcgctga aactggaaga
    14701 cgtgaagtgg ctggcgcagg gcaccatcta ccctgacgtt atcgaatctg cggcttctgc
    14761 aaccggtaaa gcacacgtca tcaaatctca ccacaacgtg ggcggcctgc cgaaagagat
    14821 gaagatgggc ctggttgaac cgctgaaaga gctgttcaaa gacgaagtgc gtaagattgg
    14881 tctggagctg ggcctgccgt acgacatgct gtaccgtcat ccgttcccgg gaccaggcct
    14941 tggcgttcgt gttctgggtg aagtgaagaa agagtactgt gacctgctgc gccgtgctga
    15001 cgccatcttc attgaagaac tgcgtaaagc ggacctgtac gacaaagtca gccaggcgtt
    15061 caccgtcttc ctgccagtac gttccgttgg cgtaatgggc gatggtcgta agtatgactg
    15121 ggttgtctct ctgcgtgctg tcgaaaccat cgactttatg accgcacact gggcgcatct
    15181 gccgtacgat ttcctcggtc gcgtttccaa ccgcattatc aatgaagtga acggtatttc
    15241 ccgcgtggtg tatgacatca gcggcaagcc gccagctacc attgagtggg aatgattttg
    15301 atccctgcac tatgaatgaa caaaaccctc cgttactaca gagggttttt tatcttcaag
    15361 aatcataggc ttgaagttac taacatcgat taattaaacc agctgtccga tttgttctct
    15421 tctgctttgc ccacgctttt catcagatcg cgaccgcctt cagtcatatt tctgtttgcg
    15481 tcagcttcag attgcaccac atcggtttgc gcagctttgt gcttcagttc ctgatcgata
    15541 aattcgtttt cgcgcttaac gcgggcttct tctttcgcca gcgccagttt ttgtttctga
    15601 atctctaagc tgcgtagctc atcttcataa ctttgatcgc gttttttgtc cgcagtggct
    15661 tcggcgtcca gtttatcctg acgagctttc ttatttgccg ctgccgttgc cgctcttttg
    15721 ttcgcggcgg cctgggcatt tgcgcgacgt tgcttctctt gctggatttc cctgttgcgc
    15781 tccgcgaccc attcgtcatg ctgcctttgc tcttcatttt taccttgctg ttccgcttct
    15841 gcgacagcag agagttgatc ctgcaatgat gaggctatag ccggatagct taaggaggct
    15901 aagatggcgc aaagaaaaac tttcttcatg actcctcctg attattagct cttttcagga
    15961 catttggtat ttggctgaat acgcgtttcg ttatacgttg tggtaataac aacggctaaa
    16021 cctgtcgtaa actggcactc tttaccctcc tgggtggagg tatacacttt ggtgccttcc
    16081 ttatatgtta aagaaacacc ttccactaag gttttatcat tcaccataga acccgctgcc
    16141 gcgcctacag ctccgccgcc aactgcccct gccgtcgttc cggaattgct gccagaaccg
    16201 acgttgtggc cgataacacc gccagcgact gcgccaataa gcgcgccgaa ggcttgtgcg
    16261 ttccgtttat tttgggagtt gtctacggca acttttgcgg gaagaatgga aataatatta
    16321 acggttttag tttcttgttt ggtattcagt tgatcggttt gataaacatc ggcggcatga
    16381 tcgtcagcat ttgactggca tcctgccaga gtgaatgacg ctaacattgc cacaggcaga
    16441 agacattttt taaatttcat cactattcct tgttatacac attacgaaaa ataagacatt
    16501 taagatcatc aaaagaggaa agtaaatgta ttgcgtataa agggatagat taaatttatt
    16561 aaaattcacg cagggaataa tttataaaaa tatatcatta caacatatat attaatttta
    16621 tatttatttc gcagccaaac aggataaaca tcagtcagga caaagacgta aaatttaaga
    16681 tgaaagaata tctgccgctt tgataatccc aaagagcaaa agcatccgac agcgaagtgc
    16741 cgttatgttc tgtgaaagtg gagaaggtaa ccgctaacgt tgtaccggtg attcgacgga
    16801 gagtgtggat tgctgagctt cttttttgct ctgatggtgc tgccaggcac cgacggacga
    16861 gtagataaaa cggccaaaga agaagataaa gctgatgagc agtacgatac gggtcatgcg
    16921 actgttaaat cggtgtcgtt ttcgcatact agttgcctga ctcacaaaag gttccttgaa
    16981 gtatgtccca cgctgtggac ggtacttaca ttaaggcaca acaggacaaa atggtcaatt
    17041 cttcgttatg taaaaaagcg tcagtggata catattttaa tgttatggaa gttaatttaa
    17101 ttatttacaa tgatgatgta ataatgatga attaacatga taaacgtaat aattcattaa
    17161 tcagggttat tttgtttgat atatatcaat tggaactttc atatgacact tagaatcaat
    17221 gctctctata gtgatgaata atcatcattc gaagtcaggt gggatgcctg tctgaataca
    17281 cctccttcag gatgtggggg attctgctga gcatctatga aactgaatgc aacttatata
    17341 aaaatacgtg ataaatggtg ggggcttccg ctgttcctgc cttctttaat cttgcccatt
    17401 ttcgcccaca ttaatacttt cgcgcatatt tcttccggtg aggtttttct cttttatctg
    17461 cctttggcac tgatgatcag catgatgatg tttttcagct gggcggcatt gccagggatc
    17521 accttaggga tttttgtccg caaatatgca gagctgggtt tttacgaaac gctctcatta
    17581 acggctaatt ttattatcat tatcattctc tgttggggcg gttacagggt ctttactccc
    17641 cggcgtaaca acgtttcaca tggtgatacc cgtttaattt cccagcgtat attctggcag
    17701 atagtgtttc ctgcaacgct gtttctgata cttttccagt ttgctgcatt tgtaggatta
    17761 ctggcgagca gagaaaatct ggtcggtgtc atgcctttta acctcgggac tttaatcaat
    17821 tatcaggcct tactggtggg taatctgatc ggtgtcccgc tgtgctactt catcattcgg
    17881 gtagtgcgaa atccatttta tttacgtagc tattattcgc aattaaaaca gcaggttgat
    17941 gccaaagtca ccaaaaaaga gttcgcgctc tggctactgg cattaggtgc tttattgttg
    18001 ctgttatgca tgccgttaaa tgaaaaaagc acaattttta gcaccaatta taccttgtca
    18061 ttattgctgc ccctgatgat gtggggagca atgcgctatg gttataagct gatttcgctg
    18121 ctctgggcgg tcgtgttgat gatcagcatc cacagctatc aaaattacat tcccatttat
    18181 cctggctata ccacgcagct gaccataacc tcctccagtt atctggtatt ctcttttatt
    18241 gtcaattata tggctgtact ggcaactcgt cagcgagcgg tagtcagacg cattcagcgg
    18301 cttgcgtatg tggacccggt ggttcatctg ccaaatgttc gcgccctgaa tcgcgcgttg
    18361 cgtgatgccc cctggtctgc gctttgttat ttacgcatcc ctggcatgga aatgctggtt
    18421 aagaactatg gcatcatgct gcggattcaa
//
LOCUS       DAETQL010000078        18046 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.078, whole genome shotgun sequence.
ACCESSION   DAETQL010000078 DAETQL010000000
VERSION     DAETQL010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.078"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            441..1124
                     /locus_tag="K0W65_002983"
     CDS             441..1124
                     /locus_tag="K0W65_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBI3096166.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1168..5830
                     /locus_tag="K0W65_002984"
                     /pseudo
     CDS             1168..5830
                     /locus_tag="K0W65_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6198..7853
                     /gene="lldP"
                     /locus_tag="K0W65_002985"
     CDS             6198..7853
                     /gene="lldP"
                     /locus_tag="K0W65_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBI3096167.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7853..8629
                     /gene="lldR"
                     /locus_tag="K0W65_002986"
     CDS             7853..8629
                     /gene="lldR"
                     /locus_tag="K0W65_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBI3096168.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8626..9816
                     /gene="lldD"
                     /locus_tag="K0W65_002987"
     CDS             8626..9816
                     /gene="lldD"
                     /locus_tag="K0W65_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBI3096169.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10012..10485
                     /gene="trmL"
                     /locus_tag="K0W65_002988"
     CDS             10012..10485
                     /gene="trmL"
                     /locus_tag="K0W65_002988"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBI3096170.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10538..11359)
                     /gene="cysE"
                     /locus_tag="K0W65_002989"
     CDS             complement(10538..11359)
                     /gene="cysE"
                     /locus_tag="K0W65_002989"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBI3096171.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11439..12458)
                     /gene="gpsA"
                     /locus_tag="K0W65_002990"
     CDS             complement(11439..12458)
                     /gene="gpsA"
                     /locus_tag="K0W65_002990"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBI3096172.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12458..12925)
                     /gene="secB"
                     /locus_tag="K0W65_002991"
     CDS             complement(12458..12925)
                     /gene="secB"
                     /locus_tag="K0W65_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBI3096173.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12988..13239)
                     /gene="grxC"
                     /locus_tag="K0W65_002992"
     CDS             complement(12988..13239)
                     /gene="grxC"
                     /locus_tag="K0W65_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBI3096174.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13381..13812)
                     /locus_tag="K0W65_002993"
     CDS             complement(13381..13812)
                     /locus_tag="K0W65_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBI3096175.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14057..15601
                     /gene="gpmM"
                     /locus_tag="K0W65_002994"
                     /gene_synonym="pgmI"
     CDS             14057..15601
                     /gene="gpmM"
                     /locus_tag="K0W65_002994"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBI3096176.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15611..16894
                     /gene="envC"
                     /locus_tag="K0W65_002995"
     CDS             15611..16894
                     /gene="envC"
                     /locus_tag="K0W65_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBI3096177.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16898..17857
                     /locus_tag="K0W65_002996"
     CDS             16898..17857
                     /locus_tag="K0W65_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBI3096178.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17844..18046)
                     /locus_tag="K0W65_002997"
                     /pseudo
     CDS             complement(17844..18046)
                     /locus_tag="K0W65_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4545 a         4663 c         4674 g         4164 t
ORIGIN      
        1 actcccttta cttcgattta ccgctggttg atattctgaa aatgcatttt taccgaaata
       61 gccatctata aacaataaaa atatatagaa gatatcattt gttaattaac aagcatttac
      121 gtctcttttt ttcggattta attgtgaaat tttaatcatc atattcacaa aatcaattat
      181 ctgtgaaatt tttcccagac atggtttctt tcatatttga catttattaa taatcatcat
      241 tagaatccac gaaaaataat atattcatac aaacacagac atcttttgaa acgaatgata
      301 catttttatg atgaatgctt aaaacaatat ttaatcgaca cacatcactg tcattaatgt
      361 aaagcaacat agctcaccaa taatacaatg gacaactcta ccggcattga gcgctggtcg
      421 gaggaaataa ggatttttac atgaacttga agaagatttt tttcagtgct gtcactgttt
      481 cagttctctg cgcattaacg ggatgtgact acattgaaga aggcaagcca gaaagtagtt
      541 tactgaaaca acaagaagaa cataataata aaattgtgct acttgaaaaa caacaagcac
      601 agctaaagag tcaactcgaa actattcaaa aacaacaaac tggcataata aacagtacaa
      661 agactttaac tcacgtaatt aaatccgtta aagaccaaca aaatactttt atttttaccg
      721 agtttaaccc agcaaaaacg aagtatttca tcctgaacaa cggttcggtg gctttagcgg
      781 gtcgagtgtt atccattgac gccacagaga atggtagtgt tattcatatc tcactggtca
      841 acttattaag tacacctatc tcaaacattg gttttaatgc gacatggggt ggtgaaaaac
      901 ctgtcgatgc caaagagttt gcccggtggc aacaattact tttcaacaca tcaatgaaat
      961 ccacattgaa attattacca ggtcaatggc aagacattaa tttgaccctg aagggtgtat
     1021 cgcccaataa cctgggatat ctgaagttag ccatcaacat ggaaaatatt cagttcgaca
     1081 atcttccctc tgctgaaaat cggcagaaaa gaagcaaaaa ataaatgcag ttagtacgct
     1141 tataacaagg aatagcgagc atcattaatg aacaaaatat ttaaagttat ctggaaccct
     1201 gcgacaggga attatactgt taccagcgaa acggcaaaaa gccgtggcaa gaaatctggg
     1261 cgcagtaagc tgttaatttc tgcgctggtt gcgggtggaa tgttgtcgtc gtttggggca
     1321 ttggcgaatg ccgggaatga caacggtcag ggtgttgatt acggtagtgg atcagctggc
     1381 gacggctggg ttgctatagg caaaggggcg aaagcaaata cttttatgaa caccagtggt
     1441 tccagtactg ctgtgggtta tgacgctata gctgaaggcc aatatagctc tgccatcggg
     1501 tcaaaaaccc atgcgattgg tggtgcatca atggcctttg gggttagtgc aatatcagaa
     1561 ggcgatagaa gtatagcact gggtgcctct tcgtattcat tgggccaata ctcaatggcc
     1621 ctcggccgtt attcaaaagc attgggtaaa ttgtctattg ctatggggga ctcttccaaa
     1681 gcggaaggag caaacgccat tgccctgggg cgtagcagtg tagctagcgg tactgacagc
     1741 ctcgcatttg gcagacaatc acttgccagc gcagcgaacg ctattgcgat aggtgctgag
     1801 accgaagccg ctgaaaatgc aactgctatt ggcaataatg cgaaggcaaa agggactaat
     1861 agcatggcaa tggggttcgg aagccttgcc gataaagtca atactatcgc attaggaaat
     1921 ggcagccagg ctctggcaga taatgcaatc gccataggcc agggcaacaa agctgatggc
     1981 gtggatgcca tcgctctggg taatggtagc cagtcgagag gcttaaacac cattgcctta
     2041 ggcacagcca gtaatgcaac tggtgataag agtcttgcgc ttggtagtaa tagcagtgcc
     2101 aacggtatta actctgtcgc gctgggcgca gattccattg cggatttaga caataccgtc
     2161 tctgtcggca atagttcatt aaaacgcaag atcgttaatg tgaaaaatgg cgcgatcaag
     2221 tctgacagtt acgatgccat taatggttca cagctttatg ccattagcga ctcggtagca
     2281 aaaaaggctt ggaggagggg ctgcagtaga tgttgatgac ggtactgtta cagcaccaac
     2341 ctacaattta aaaaatggta gcaaaaataa cgtaggggct gcgctcgctg tacttgatga
     2401 aaacaccctg caatgggacc aaaccaaagg caaatacagc gctgctcatg gtactagtag
     2461 cccaactgcc agcgtaatca ccgatgttgc ggatggcacg atttcagcct ccagtaagga
     2521 tgcggttaac ggttcccaac tgaaagctac caatgacgat gtcgaagcca acaccaccaa
     2581 tatcaccaac ctgacggatt ccgttggtga ccttcaggct gatgccctgc tctggaacga
     2641 aactaaaaag gcattcagtg cagctcacgg ccaggatacc accagcaaaa tcaccaacgt
     2701 taaagatgcc gacctgacgg ctgacagcac tgatgctgtt aacggctctc agctgaaaac
     2761 caccaacgat gctgtggcga cgaataccac caatatcgcc aataacactt ccaatattgc
     2821 cactaacacc accaacatct ctaacctgac tgagacggtg actaatcttg gtgaggatgc
     2881 gctgaaatgg gataaggaca atggtgtatt cacggcagct catggcaccg agaccaccag
     2941 caaaataacc aacgttaaag atggcgacct gacgactggc agcaccgatg ccgttaacgg
     3001 ctctcagctg aaaaccacca acgatgccgt ggcgacgaat accaccaata tcgccactaa
     3061 caccaccaac atctctaatc tgactgagac ggtgactaat cttggtgagg atgcgctgaa
     3121 atgggataag gacaatggtg tcttcactgc agctcatggc aacaataccg ccagcaaaat
     3181 caccaatatc ctggacggca cagtcactgc aaccagttcc gatgccatta acggtagcca
     3241 gctttatgac ttaagcagca atatcgccac ctacttcggc ggcaatgctt ctgtgaatac
     3301 tgacggtgtg tttaccggtc caacctacaa aatcggtgaa acaaattatt ataacgtcgg
     3361 cgatgcactg gctgcgatta actcctcatt tagcacgtct ctcggcgatg ctctgctttg
     3421 ggatgccacc gcaggtaaat tcagtgccaa acacggtact aatggtgacg caagcgtgat
     3481 cactgatgtc gcagatggtg aaatttcaga ctccagttct gacgcagtaa acggctcaca
     3541 actccacggc gtgagcagtt atgttgttga tgcgctgggg ggtggtgccg aagtcaatgc
     3601 agacggcacc atcactgcgc cgacgtacac cattgctaat gctgattacg ataatgtcgg
     3661 tgatgccctg aatgctatcg ataccactcc tgacgacgct ctgctctggg atgcggacgc
     3721 cggtgaaaat ggtgcattta gcgccgctca cggaaaagat aaaactgcca gtgtaatcac
     3781 taacgtcgct aacggtgcaa tctctgctgc cagcagcgac gcgattaacg gctcacaact
     3841 ctataccacc aataagtaca tcgctgatgc gctgggtggt gacgcagaag tcaacgctga
     3901 cggcaccatc accgcaccga cttacaccat tgcgaacgcc gagtacaaca acgtcggtga
     3961 cgccctggat gcgcttgatg ataacgccct gctgtgggat aagactgcca atggcggtgc
     4021 tggagcctac aatgccagcc atgacggtaa agccagcatc atcactaatg tcgctaatgg
     4081 cagtattagt gaggacagta ccgatgcagt gaacggttct cagttgaatg cgacgaatat
     4141 gatgattgag cagaacaccc aaattatcaa tcagctcgct ggtaacaccg acgcaaccta
     4201 tatcgaagaa aatggcgctg gtatcaacta cgttcgtact aacgacaacg atttagcctt
     4261 taacgatgca agcgcctctg gtgtcggcgc tacagctgta ggttataacg ctgtcgcgtc
     4321 tggtgccagc agcgtagcca ttggtcagaa cagcagcagc accgttgata ccggtattgc
     4381 gctgggtagc agctccgttt ccagccgtgt gattgccaaa ggttctcgtg acactagcgt
     4441 aacggaaaat ggcgtggtga ttggttatga caccactgac ggcgaactgc taggtgcatt
     4501 gtcaattggt gatgacggta aataccgcca aatcatcaac gtagctgatg gttcagaagc
     4561 ccatgacgcc gttacggttc gccagttgca gaacgctatt ggagcggtcg ccactacgcc
     4621 aaccaagtac tttcacgcca actcaacggc agaagactca ctggccgttg gtgaagactc
     4681 gctggcaatg ggtgcgaaaa ctgtcgttaa tggtaatgca ggtattggta tcggtttgaa
     4741 cactctggtt ctggctgatg cgatcaacgg cattgctatc ggcagtaacg cacgcgcaaa
     4801 ccatgcaaac agtatcgcaa tgggtaacgg ttctcagacc acccgtggtg cacagactgg
     4861 ctacaccgcc tacaacatgg acgcaccgca gaactctgta ggtgagtttt ctgtcggtag
     4921 tgaagacggt caacgtcaga tcacaaacgt cgcagctggt tcagcggata ccgatgcggt
     4981 taacgtgggt cagttgaaag tcactgatga gcgcgtagcg caaaataccc agagcattac
     5041 taacctgaac aatcaggtca ctaatctgga tactcgcgtt actaatatcg aaaacggtat
     5101 tggcgacatt gtcaccaccg gtagcaccaa gtacttcaag accaacaccg atggcgtaga
     5161 tgccaacgcc cagggtaaag atagcgttgc tattggttct ggttccattg ctgccgctga
     5221 caacagcgtc gcactgggta ccggttccgt tgcagaggaa gaaaatacaa tctctgtagg
     5281 ttcttctact aaccaacgcc gtatcaccaa cgtagctgcc agtgttaatg ccaccgatgc
     5341 ggttaacgtg tcgcagctga aatcttctga agcaggcgga gtgcgctacg acaccaaagc
     5401 tgatggttct atcgactata gcaatatcac cctcggtggt ggcaacggca gtacgactcg
     5461 tatcagcaac gtctccgctg gcgtcaacaa caacgacgcg gtgaactacg cgcagttgaa
     5521 gcaaagcgcg caggaaacga agcaatacac cgatcagcgg atggttgaga tggataacaa
     5581 actgtctaaa actgaaagca agttgagcgg tggtatcgct tctgcaatgg caatgaccgg
     5641 tctgccgcag gcttatacac cgggtgccag catggcttct attggtggcg gtacttacaa
     5701 cggtgaatcg gcagttgctt taggtgtatc gatggtgagc gccaatggtc gttgggtcta
     5761 caaattacaa ggtagtacca atagccaggg tgaatactcc gccgcactcg gtgccggtat
     5821 tcagtggtaa tgatccatta agttagtgtg actaagggca aacgccaaat tgcctgatgc
     5881 gttacgcgca tcaggcaaga taaggcgctc cgggtcaaca atctaagggc aattctctga
     5941 tgaggattgc ccttttcttt accagacatc tccccccaca agaattggcc ctaccaattc
     6001 ttcgcttatc tgacctctgg ttcacaattc cccaattaaa actcacatca atattgccaa
     6061 tacataacat ttagttaacc attcattgtc attatcccta cacaacacaa ttggcagtgc
     6121 cacttttaca caacgtgtga caaggagatg agcaacagac tcattacacg atgtgcgtgg
     6181 actctaggag acctgcaatg aatctctggc aacaaaacta cgatcccgcc gggaatatct
     6241 ggctttccag tctgatagca tcgcttccca tcctgttttt cttctttgcg ctgattaagc
     6301 tcaaactgaa aggatacgtc gccgcctcat ggacggtagc aatcgccctt gccgtggctt
     6361 tgctgttcta taaaatgccg gtcgctaacg cgctggcctc ggtggtttat ggcttcttct
     6421 atggtttgtg gcccatcgcg tggatcatta ttgcagcagt gttcgtctat aagatctcgg
     6481 tgaaaaccgg gcagtttgac atcattcgct cgtctattct ttcgataacc cctgaccagc
     6541 gtctgcaaat gctgatcgtc ggtttctgtt tcggcgcttt ccttgaagga gccgcaggct
     6601 ttggcgcacc ggtagcaatt accgccgcat tgctggtcgg cctgggtttt aaaccgctgt
     6661 acgccgccgg actgtgcctg attgttaaca ccgcgccagt ggcatttggc gcgatgggca
     6721 ttccaatcct ggttgccgga caagtaacag gcatcgacag ctttgagatt ggccagatgg
     6781 tggggcgtca gctaccgttt atgaccatta tcgtgctgtt ctggatcatg gcgattatgg
     6841 acggctggcg cggtatcaaa gagacgtggc ctgcggtcgt ggttgcaggc ggctcgtttg
     6901 ccatcgctca gtaccttagc tctaacttca ttgggccgga gctgccggac attatctctt
     6961 cgctggtatc actgctctgc ctgacgctgt tcctcaaacg ctggcaacca gttcgcgtat
     7021 tccgcttcgg tgacttgggg gcgtcacagg ttgatatgac gctggcccac accggttaca
     7081 ccgcgggtca ggtgttacgt gcctggacac cgttcctgtt cctgaccgct accgtaacac
     7141 tgtggagcat cccgccgttt aaagccctgt tcgcatcggg tggtgcgctg tatgagtggg
     7201 tgatcaacat tccggtgccg tacctcaata aactggttgc ccgtatgcca ccagtggtca
     7261 gcgaggctac agcctatgcc gccgtgttta agtttgactg gttctctgcc accggcaccg
     7321 ccattctgtt tgctgcactg ctctcgattg tctggctgaa gatgaaaccg tctgacgcta
     7381 tcagcacctt cggcagcacg ctgaaagaac tggctctacc catctactcc atcggcatgg
     7441 tgctggcgtt cgcctttatc tcgaactatt ccggactgtc atcaacactg gcactggcac
     7501 tggcgcacac cggtcatgca ttcaccttct tctcgccgtt cctcggctgg ctgggggtat
     7561 tcctgaccgg gtcggatacc tcatctaacg ccctgttcgc cgcgctgcaa gccaccgcag
     7621 cacaacaaat tggcgtctct gatctgttgc tggttgccgc caataccacc ggtggcgtca
     7681 ccggtaagat gatctccccg caatctatcg ctatcgcctg tgcggcggta ggcctggtgg
     7741 gcaaagagtc tgatttgttc cgctttactg tcaaacacag cctgatcttc acctgtatgg
     7801 tgggcgtgat caccacgctt caggcttatg tcttaacgtg gatgattcct taatgattgt
     7861 tttacccaga cgcctgtcag acgaggttgc cgatcgtgtg cgggcgctga ttgatgaaaa
     7921 aaacctggaa gcgggcatga agttgcccgc tgagcgccaa ctggcgatgc aactcggcgt
     7981 atcacgtaat tcactacgcg aggcgctggc aaaactggtg agcgaaggcg tgctgctcag
     8041 tcgacgcggc ggcgggacgt ttattcgctg gcgtcatgac acatggtcgg agcaaaacat
     8101 cgtccagccg ctaaaaacgc tgatggccga tgatccggat tacagtttcg atattctgga
     8161 agcccgctac gccattgaag ccagcaccgc atggcatgcg gcaatgcgcg ccacacctgg
     8221 cgacaaagaa aagattcagc tttgctttga agcaacgcta agtgaagacc cggatatcgc
     8281 ctcacaagcg gacgttcgtt ttcatctggc gattgccgaa gcctcacata acatcgtgct
     8341 gctgcaaacc atgcgcggtt tcttcgatgt cctgcaatcc tcagtgaagc atagccgtca
     8401 gcggatgtat ctggtgccac cggttttttc acaactgacc gaacaacatc aggctgtcat
     8461 tgacgccatt tttgctggtg atgccgacgg ggcgcgtaaa gcaatgatgg cgcaccttag
     8521 ttttgttcac accaccatga aacgattcga tgaagatcag gctcgccacg cacggattac
     8581 ccgcctgccc ggtgagcata atgagcattc gagggagaaa aacgcatgat tatttccgca
     8641 gccagcgatt atcgcgccgc agcgcaacgc attctgccgc cgttcctgtt ccactatatg
     8701 gatggtggtg catattctga atacacgctg cgccgcaacg tggaagattt gtcagaagtg
     8761 gcactgcgcc agcgtattct gaaaaacatg tccgacttaa gcctggaaac gacgctgttt
     8821 aatgagaaat tgtcgatgcc ggtagcactg gcaccggtgg gtttgtgtgg catgtatgcg
     8881 cgtcgtggcg aagttcaggc agccaaagcg gcggacgcgc atggcattcc gtttactctc
     8941 tcgacggttt ccgtttgccc gattgaagaa gtcgcgccag ccatcaagcg cccaatgtgg
     9001 ttccagcttt atgtactgcg cgatcgcggc tttatgcgta acgcgctgga gcgagcaaaa
     9061 gcagcgggtt gttcgacgct ggttttcacc gtggatatgc cgacaccggg cgcacgctac
     9121 cgtgatgcgc attcaggtat gagcggccca aatgcggcaa tgcgccgcta cttgcaggcg
     9181 gtgacgcatc cgcaatgggc gtgggatgtg ggcctgaacg gtcgtccaca tgatttaggt
     9241 aatatctcag cttatctcgg caaaccgacc ggactggaag attacatcgg ctggctgggg
     9301 aataacttcg atccgtccat ctcatggaaa gaccttgagt ggatccgcga tttctgggat
     9361 ggcccgatgg tgatcaaagg gatcctcgat ccggaagatg cgcgcgatgc agtacgtttt
     9421 ggtgctgatg gaattgtggt ttctaaccac ggtggccgcc agctggacgg tgtactctct
     9481 tccgctcgtg cgttgcccgc tattgcagat gcggtgaaag gtgatatcgc cattctggcg
     9541 gatagcggaa ttcgtaacgg gcttgatgtc gtgcgtatga ttgcgctcgg tgccgacacc
     9601 gtactgctgg gtcgtgcttt cctgtatgcg ctggcaacag cgggccaggc gggtgtagct
     9661 aacctgctaa atctgatcga aaaagagatg aaagtggcga tgacgctgac tggcgcgaaa
     9721 tcgatcagtg aaattacgca agattcgctg gtgcaggggc tgggtaaaga gttgcctacg
     9781 gcactggctc cgatggcgaa agggaatgcg gcatagtcgt ttgcgccccc tcaccctaac
     9841 cctctccctc agggagaggg gaccgttcgg cgctgtatgt actccctcac tctgaaacga
     9901 caccgcactc tttttttctc cctcgcccct ccggggaggg ccggggtgag gggaaaaggc
     9961 cgcacggttc accaattttc tgctatcctg cccccgcact aagggggcag tatgctaaac
    10021 atcgtacttt acgaaccaga aattccgcca aatactggca acatcatccg tctttgcgct
    10081 aataccggct ttcgtctaca tatcatcgaa ccgatgggat ttgcctggga cgacaagcgc
    10141 ctgcgccgcg cggggctgga ctatcacgag tttaccgccg ttacgcgtca tcatgactat
    10201 cgcgcgttcc tcgaagcaga aaatccccag cgcctgtttg ccctcaccac gaaaggtacg
    10261 cctgctcaca gcgccgtaag ctatcaggat ggcgattatc tgatgttcgg cccggaaaca
    10321 cgcggcctgc cagcgagcat tcttgatgcc ctgcccgctg aacaaaaaat tcgcattccg
    10381 atggtgccgg acagccgcag catgaatctg tccaatgcgg tgtcggtagt ggtgtatgaa
    10441 gcctggcggc agctgggata tccgggagcg ttgttgagag attagttact gtatgccgga
    10501 tagatgatta catcgcatcc ggcacgatca caggacatta gatcccatcc ccatactcaa
    10561 atgtatggtt aataccgttg aaatgctggt ccatatccat tgatggctta tcgctgtctg
    10621 gtttaccgac aatacgagcc ggaacgccag cggcggttgt atgcggcggc accggttgca
    10681 gcaccacgga acctgcgcca atcttcgcgc cgcgaccaac ttcaatattg ccgaggattt
    10741 tcgcgcccgc gccaatcatc acaccttcac gaattttcgg gtgacggtca ccaccagatt
    10801 tacccgtacc gccaagcgtc acagattgca gaatcgatac gtcgttttca attaccgccg
    10861 tttcacccac aacgatgccc gtggcgtggt cgagcataat gccacggcca atttttgctg
    10921 ccgggtgaat atcgacctgg aacgtcacag aaacctggtt ttgcagaaag attgccaatg
    10981 cgcgacgccc ctgattccac aaccagtgac cgatgcgata ggcctgcaag gcatgaaaac
    11041 ccttcaggta taacaacggg gttgagtatt tatcgactgc cgggtcgcgg gtacgcaccg
    11101 cctgaatatc acaggccgca gaggcgatca tttccgggtc agcggcgtag gcttcttcaa
    11161 ccacttcacg gatagcaata gcaggcataa ttggcgatga cagcttgttc gccagcatgt
    11221 agctcagtgc actgccaagg ttttcgtgct tgagtagcgt cgcgtggtaa aaactggcca
    11281 gcattggctc acagtccgcc agcgttctgg cttcggcttt aatattgttc cagacaattt
    11341 ccagttcttc acacgacatt gcttactcca cacgatgaga taatgaccgg cccgttctgc
    11401 gcgggccggg tcatagcggt aacaaaggtt ccctggggtt agtgactgct gcgctcgtcc
    11461 ttgcgtgcac gacctaataa tgtcaatgct gcctcgcgcg cgttttttcc gcaatataat
    11521 acttgataaa tttcctcggt tattggcatt tcaacgccga agcgatgcgc cagttcgcgg
    11581 acttctttcg tattgcggta gccttccacc acctgaccaa tcttctcctg cgcgctttgt
    11641 acatccatgc cctgaccgag catcatacca aaacggcggt tacgcgactg gttgtcggta
    11701 caggtaagca ccagatcgcc cagccccgcc atgcccataa aggtggcagg atcggcaccc
    11761 agcgccgcac caagacgcga catttcagcc agcccacggg tgatcagcgc cgtacgcgca
    11821 ttcgcaccaa aaccgatacc gtcagacatc cccgcgccaa tggcaatgac gtttttcacc
    11881 gcgccgccaa gctgcacgcc aatgaaatcc gggttgctgt aaacgcggaa acttttgccg
    11941 cagtgcagca gctgctggag atcatcggca aaggtctgat cggtcgaggc cagcgaaata
    12001 gctgtcggta aacctgccgc cagttctttc gcaaacgttg ggccagagat aaccgccagc
    12061 ggaatttgat cgcctaaggc ctcacgcgcc acgtcctgta acagacgtcc ggtttccgct
    12121 tccagccctt tggtcgccca caccagacgc gcatcaggac gcatcagcgg tttaatctgg
    12181 cgcagcactt caccaaagac atggctgggt acgacgacga gaatattacg actggctgcc
    12241 agcgcagtgg cgagatcgct ttcaagatgg agcgtatcgg gaaaaggcac atcggggaga
    12301 aacgcggcgt tacagcggtc gcgttcaagc gttgcgatat gttcagggtc atggccccag
    12361 aggacaacct cgtggccatt tcttgccagg gtaatggcaa gagcggtgcc gtacgagccg
    12421 gcaccgatca cagtcattga agcattacgt tggttcatca ggcatcctga tgttcttcag
    12481 taccttcgcc agcctgctgc tgcaaatagt tcatgaacag cgcatcgaag ttaaccggcg
    12541 caaggttcag ttgcgggaat gtaccgcggg ataccatgct ggtgatgcac tcacgagcat
    12601 acgggaacag aatgttcggg cagtatgcgc ccaggcaatg tgccatctgg gtgccttcga
    12661 tacccgcgat ggagaaaata ccgccctgct gaacttcaca caggaacgcg gtttcttcgc
    12721 ccaaggaggc cgttacggta acacgcagta ccacttcgta tacgtcatct gccagttggg
    12781 aagatgccgt atccagatca agtttaactt ctggttgcca atctttctgg aaaacgtgcg
    12841 gcgcgttcgg cgcttcgaaa gagatatcct tggtataaat acgttggatc tggaaagtca
    12901 tttcagtgtt gttttgttct gacatgtgta gaaaaccctt aagtgttgtc cttaaatact
    12961 gcgcaatgta ataccaacgt tcacacgtta tttcagcagg ggatccagtc caccacgtgc
    13021 atccaatgca tacaagtcat cacagccgcc aatgtgctgt gcgtcaataa aaatctgggg
    13081 aaccgtggtg caaccgctgc gtttgatcat ctcttcacgc ttggcggcat tgccatcgat
    13141 cggcaactcc tggaaactca cgcccttgct gctcagcagt gcttttgcac gatggcaata
    13201 cgggcaggtt tctttggtat agatttcaac attggccata atttagctcc tgttttttta
    13261 ccctgaaatt tcatgttgca gggcggctgc aaaatggagt gtccccagaa gcttataaca
    13321 ataagtcgct gggggcctaa ttgcagctaa cggcctgcat catgaaagac gacaggtaaa
    13381 ttatttgccg cgcaccagag gcaagttttc gccagcccag ccagcaacgc cctctttcag
    13441 cacgaatact tgcgcaaaac cagctttcgt cagcgcgttt gcaggctcct ggcactgcat
    13501 gccagaaccg tctaccacga taaccgattt gtctttgtgc ttctcaagct caccaacatt
    13561 gttggctttg atttcgctcg gcaacagatt gatagaacct gcgatatggc ctttacggaa
    13621 gtcatcacgc tgacgtaaat ccacaaccac agcgtcttct ttgttgatca gacgcgtagc
    13681 ttcaccacga gtaatcacct tcactttcga ggtcaggctc ttaaacgtag tcacaagaac
    13741 cgccaccagt aacgcgatcc aggcgatact cagtatggga tgacggccaa caaattgcat
    13801 aatttcttgc atggggggta acaactcccg acgtagtgat taaaaaaacc aggaaaggag
    13861 tatacctgcg cggtgcggca aatacagcca gcgcgttaac tggaatgcaa ttttgcgggg
    13921 cacgacgaaa aaaaagcgca aatctggcat cggcttgccg cgagcggtcg tatattttga
    13981 tcttcagagg ctattttatc gattcagctg tagtaaaatt acgcaaattt tgactcttga
    14041 gtatgaggtt gtcgcaatgt cggtttctaa aaaacctatg gtactggtga ttctggatgg
    14101 ctatggctat cgtgaagaac agcaggataa cgccattttt agtgctaaaa ccccggtaat
    14161 ggatgcactg tgggccaatc gtccgcatac cctgatcgac gcttccggtc tggaagtcgg
    14221 tctgcctgac cgtcagatgg gtaactccga agtaggccac gttaacctgg gtgccggccg
    14281 catcgtgtat caggacctga ctcgtctgga cgttgaaatc aaagatcgcg ctttctttgc
    14341 taatccagtg ctggctggcg cggtacataa agcgaaaaac gcaggtaaag cagtacacat
    14401 tatgggtctg ctctccgcag gcggcgtaca cagccacgaa gatcacatca tggcgatggt
    14461 agaactggca gctgaacgcg gcgcagaaaa aatctacctg cacgcattcc ttgacggtcg
    14521 cgacaccccg ccgcgcagtg ctgaatcctc gctgaaaaaa tttgaagaga aatttgctgc
    14581 gctgggtaaa ggccgcgtag cgtccatcat tggtcgttac tacgcgatgg accgcgataa
    14641 ccgttgggat cgcgttgaaa aagcctatga cctgctgact ctggcgcagg gtgagttcca
    14701 ggccgatact gccgttgctg gtttgcaggc agcttatgct cgcgacgaaa acgacgagtt
    14761 cgtgaaagcg accgttattc gtgcagaagg tcagccagat gcggcaatgg aagacggcga
    14821 tgcgctgatt ttcatgaact tccgtgctga ccgcgcgcgt gaaatcactc gtgcattcgt
    14881 gaacgctgat ttcgatggct tcgcgcgtaa gaaagtggtt aacgtcgatt tcgtgatgct
    14941 gaccgaatac gccgctgaca tcaaaactgc ggttgcttac ccacccgctt ccctggttaa
    15001 caccttcggc gagtggatgg cgaaaaacga caaaactcag ttgcgtattt ccgaaaccga
    15061 aaaatatgcc cacgttactt tcttcttcaa cggtggcgta gaagagtcgt tcaaaggcga
    15121 agatcgcatt ctgatcaact caccgaaagt ggctacctac gatctgcaac cggaaatgag
    15181 ctccgcagag ctgaccgaaa aactggttgc ggccatcaag agcggcaaat acgacaccat
    15241 tatctgtaac tatccgaacg gcgacatggt aggtcacacc ggggtgatgg aagcggcggt
    15301 taaagcggtt gaagcactgg atcactgcgt ggaagaagtc gcgaaagcgg ttgagtccgt
    15361 gggtggacaa ctgctgatca ccgctgacca cggtaacgct gaacagatgc gcgatccggc
    15421 aacgggtcag gcacacacgg cacacaccaa cctgccagtt ccgctgattt acgttggtga
    15481 taagaacgtg aaagcggttg caggcggcaa actttctgac atcgcgccga ccatgttgtc
    15541 gctgatgggt atggaaatcc cgcaagagat gactggtaag ccgctgttca tcgtggaata
    15601 atccctcccc atgaggggaa aggcgattaa taccatgaca cgggccgtga aaccgcgcag
    15661 gtttgcaatc aggcccatca tctacgccag cgttcttagc gctggcgtat tgttgtgcgc
    15721 cttttccgcc cacgcggatg agcgtgacca actcaaatct attcaggccg atatcgccgc
    15781 aaaagagcgc gcggtacgcc aaaagcaaca acaacgcgca agcctgctcg cacaattgaa
    15841 aaagcaggaa gaagcgatct ctgaagccac ccgtaagctg cgcgaaacgc aaaacacgct
    15901 caatcaactc aataaacaga ttgatgagat gaacgcgtcg attgccaaac tggagcagca
    15961 aaaagccgcc caggagcgca gcctcgccgc acaactggat gccgcattcc gtcagggcga
    16021 gcataccggt attcagctaa ttctcagcgg tgaagaaagc cagcgtggac agcgtttaca
    16081 ggcttatttc ggctatctca accaggcgcg acaagaaacc attgcccagt tgaagcaaac
    16141 gcgtgaagaa gtcaccatgc agcatgctga actggaagag aaacaaagcg agcaacaaac
    16201 gctgttatat gaacagcgcg cccaacaggc gaaactgact caggctctga acgagcgtaa
    16261 aaagacgctg gcggggctgg aatcttccat ccagcaaggt cagcagcagc taagtgaact
    16321 gcgtgccaac gaatcccgtc tgcgtaacag cattgcccgt gctgaagccg cggcgaaagc
    16381 gcgtgctgaa cgagaagcgc gtgaggccca ggcggttcgc gagcgccaga aagaagcgac
    16441 gcgcaaaggc accacctaca aaccgaccga aagcgaaaaa tcgctgatgt cccgaactgg
    16501 tggcctgggt gcgccgcgtg gtcaggcatt ctggccggtt cgcgggccga cgctgcatcg
    16561 ctatggcgag cagctacagg gtgaattacg ctggaaaggg atggttatcg gtgcctctga
    16621 aggtactgaa gttaaagcaa ttgccgacgg tcgagtgatt ctggctgact ggctgcaagg
    16681 ttacggtctg gtggtggtgg ttgagcacgg taaaggcgac atgagtcttt acggctataa
    16741 tcagagcgca ctggtgagcg ttggttcgca ggttcgcgcg ggccagccaa ttgcactggt
    16801 gggcagcagt ggcggtcagg gtcggccttc actctatttc gaaattcgcc gccagggtca
    16861 ggcggtcaat ccacagccgt ggttgggaag ataagttttg tttccatttc gtcgtaacgt
    16921 tcttgcattt gccgctctgt tggcgctctc ctcccccgta cttgctggca aacttgccat
    16981 cgtcattgat gattttgggt atcgcccgca caacgaaaac caggtgctgg cgatgccttc
    17041 cgctatctcc gtcgctgtat tacccgattc accgcacgcc agagagatgg cgaccaaagc
    17101 gcataacagc ggtcacgaag tgttgattca tctcccgatg gcaccgttga gtaaacagcc
    17161 gctggagaaa aatacgctac gcccggagat gagcagcgac gaaattgagc gcattattcg
    17221 tagtgcggtc aataacgtgc cctatgccgt ggggatcaac aaccacatgg gcagcaagat
    17281 gacctctaac ctgtttggta tgcagaaagt gatgcaggcg ctggagcgtt acaatcttta
    17341 cttcctcgac agcgtaacca tcggtaatac ccaggcgatg cgcgccgcgc aaggtactgg
    17401 cgtgaaggtg atcaaacgga aggtgttcct cgacgattcg cagaacgaag cggacatccg
    17461 tgtgcaattt aatcgcgcaa ttgatctggc gcgtcgcaac ggttcaacca ttgccattgg
    17521 gcatcctcac ccttcaacgg tacgcgtgct acaacagatg gtttataacc tgccgccaga
    17581 cattacgctg gtgaaagcca gcagcttgct gaatgaaccg caggttgata cttctacacc
    17641 gccgaaaaac gctgtgcctg atgcaccgcg taatccgttc cgtggcgtga agctgtgcaa
    17701 accgaagaaa ccgatagaac ctgtttatgc taatcgcttc tttgaagtat taagcgaaag
    17761 catcagccag agcacgctga tcgtttactt ccagcatcag tggcaaggct ggggcaaaca
    17821 gcccgaagcg gcgaagttta acgctagcgc aaattaaagg cgctacgcgt aatgcgatgg
    17881 tgcgaccgcg ttttgtcgcg ccattgccat aagcggaaag accacagtaa cgcctggtaa
    17941 ccgaccttca cgctgcgcac attggtaatc aggcgcttat acatacccgg aagtaaatat
    18001 ctcggcaatc atccgttggc gggtaagaat atccggctct ttgcgc
//
LOCUS       DAETQL010000079        17647 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.079, whole genome shotgun sequence.
ACCESSION   DAETQL010000079 DAETQL010000000
VERSION     DAETQL010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17647
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.079"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..674)
                     /gene="chbR"
                     /locus_tag="K0W65_002998"
                     /pseudo
     CDS             complement(1..674)
                     /gene="chbR"
                     /locus_tag="K0W65_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(682..1032)
                     /gene="chbA"
                     /locus_tag="K0W65_002999"
     CDS             complement(682..1032)
                     /gene="chbA"
                     /locus_tag="K0W65_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBI3096179.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1091..2439)
                     /gene="chbC"
                     /locus_tag="K0W65_003000"
                     /pseudo
     CDS             complement(1091..2439)
                     /gene="chbC"
                     /locus_tag="K0W65_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2524..2844)
                     /gene="chbB"
                     /locus_tag="K0W65_003001"
     CDS             complement(2524..2844)
                     /gene="chbB"
                     /locus_tag="K0W65_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBI3096180.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3143..3481)
                     /gene="osmE"
                     /locus_tag="K0W65_003002"
     CDS             complement(3143..3481)
                     /gene="osmE"
                     /locus_tag="K0W65_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBI3096181.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3683..4510
                     /gene="nadE"
                     /locus_tag="K0W65_003003"
     CDS             3683..4510
                     /gene="nadE"
                     /locus_tag="K0W65_003003"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBI3096182.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4740..5627
                     /gene="cho"
                     /locus_tag="K0W65_003004"
     CDS             4740..5627
                     /gene="cho"
                     /locus_tag="K0W65_003004"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBI3096183.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5587..6162)
                     /gene="ves"
                     /locus_tag="K0W65_003005"
     CDS             complement(5587..6162)
                     /gene="ves"
                     /locus_tag="K0W65_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBI3096184.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6365..6850)
                     /gene="spy"
                     /locus_tag="K0W65_003006"
     CDS             complement(6365..6850)
                     /gene="spy"
                     /locus_tag="K0W65_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBI3096185.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7180..8148)
                     /gene="astE"
                     /locus_tag="K0W65_003007"
     CDS             complement(7180..8148)
                     /gene="astE"
                     /locus_tag="K0W65_003007"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBI3096186.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8141..9474)
                     /gene="astB"
                     /locus_tag="K0W65_003008"
                     /pseudo
     CDS             complement(8141..9474)
                     /gene="astB"
                     /locus_tag="K0W65_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9471..10949)
                     /gene="astD"
                     /locus_tag="K0W65_003009"
     CDS             complement(9471..10949)
                     /gene="astD"
                     /locus_tag="K0W65_003009"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HBI3096187.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10946..11980)
                     /gene="astA"
                     /locus_tag="K0W65_003010"
     CDS             complement(10946..11980)
                     /gene="astA"
                     /locus_tag="K0W65_003010"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HBI3096188.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11977..13197)
                     /gene="astC"
                     /locus_tag="K0W65_003011"
                     /pseudo
     CDS             complement(11977..13197)
                     /gene="astC"
                     /locus_tag="K0W65_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13643..14449
                     /gene="xthA"
                     /locus_tag="K0W65_003012"
     CDS             13643..14449
                     /gene="xthA"
                     /locus_tag="K0W65_003012"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBI3096189.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14616..15326
                     /locus_tag="K0W65_003013"
     CDS             14616..15326
                     /locus_tag="K0W65_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBI3096190.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15331..16008
                     /gene="ydjY"
                     /locus_tag="K0W65_003014"
     CDS             15331..16008
                     /gene="ydjY"
                     /locus_tag="K0W65_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBI3096191.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16023..16730
                     /locus_tag="K0W65_003015"
     CDS             16023..16730
                     /locus_tag="K0W65_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBI3096192.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16730..17278
                     /locus_tag="K0W65_003016"
     CDS             16730..17278
                     /locus_tag="K0W65_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBI3096193.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17288..17614
                     /locus_tag="K0W65_003017"
                     /pseudo
     CDS             17288..17614
                     /locus_tag="K0W65_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4367 a         4687 c         4307 g         4286 t
ORIGIN      
        1 atctcattaa taatctgcat tggtgttttg ccataatatc gttgagtcgc tcgcgtcaaa
       61 tattcctgtg atttggctga caacgtcacc atattctcca gcgccgattc actaaactgc
      121 tctttatcat gcatcttttc taccgtactt ttcagccact acggtacatc atcaatcacc
      181 tgttcttcgc ggtaatgacg taaacggtta atgacataaa aagtaaccat ctcaacaaac
      241 tcttccagcc ctgtttcgcg gaaatttaat gaagaaatca ctgtttccac ataggtgaga
      301 aaagcgttat tggtccggta tacctgcgaa gcaacaaagc aataaggcaa caacggcagg
      361 taatgctgct caaaaaagcg tttactgatc ccaacgttca atatgcgcgt ggcaccaaac
      421 tcataaaaac tttgatggtg cgaacctaac ggaataaaaa caaaatcgcc ccgttccagt
      481 aacacgcgct taccgttaat ctcctggaaa taacgcccgg ttaataccag agtaaattca
      541 taatagtcgt gctgatgcag tccgctgata ctctcagttt tgttatagat aaacacatgg
      601 aagtttttgc cattaaacaa ctgctgttct cgggcagtgg caatttccgg cgcgttaatc
      661 actggctgca tcatcgactc cttatgcctt cagtttttca tgaagctcaa ttaattcagt
      721 aatcagttca cgcgcaagca tggacgtcat taaatgatcc tgagcgtgga ccagcaccag
      781 actcactttc atcttacctt cgcccgcatc gccttcaatc agtttcgtct gtaccagatg
      841 cgcttcattc aatgccatcc gtgactgatc catcatggct ttcgctgcgg caaaatcgcc
      901 ctgcttcgcc tgtttcaatg cggcatacgc caggctacgc gcttgtccag agttgatgat
      961 aagccccatc accacttctt ccagctcttc agcttctgtt tgcgtatcag gaatattatc
     1021 gagatccatc atacatcgtt cctctctctt cttaccggca agattacccg taccggcatc
     1081 gattaaaatt tcaggggtta gcgatatctt cttcgctctc ttctttatca atcgcatttt
     1141 gtgctttgtt agccaccaca acaaagggca gataaattaa cgttgcgatg ccaaggttga
     1201 agagtgcgac cagcaatgcg gcgacgctac cgttggtgtt aaagaaggct cccagaccgg
     1261 ttggcatggt ccacggtgca atattggtca ccggaggaat gatgcccatg tagtacgctg
     1321 cgagggtgat tgccgccaga atcggttgta ccagtacaaa cgggataaac atcaccgggt
     1381 tcatgataat tggcagacca aacagaatcg gttcattaat ctggaagatg ccggacggca
     1441 gcgccagttt tgccacctga cgataatcag cacggcgaga ggcgataaag atagccagga
     1501 tcaggcctaa agtcgcacca ctgcccccaa ggaaaataaa ggagtccagc atcggcttgg
     1561 cccagatatg gaaggtctta ccggctgcca gcgccgcttc gacggaacca tattgctgat
     1621 aggtcgcgat attttccagc gcccacggcg tcataatgcc gttgtccagt gcggtcagcg
     1681 ccagcgcgcc atgaataccg aagaaccaga gcagtggaac aaagatcaca taggcccagc
     1741 ccaccacgct acccaacgat gccagtgggg ttgagatggt atccataatg atctgatgga
     1801 agttggtgcc ccaggtattc aacgcccagg caataatccc catcacggaa agaataataa
     1861 agccgggaat taatgccgag aaagaacgcg atactgaagc aggtacgctg tcgggcagtt
     1921 taatgaccca attgcggcgg acaataaagg taaacatttc tgccaccacc aggccaataa
     1981 taatcccgga gatgatattc gccccaccta accagtttgc gccaaccgca taggcctcac
     2041 cgacactata tggggtgacg gtcataaatg ctgcaacgga taacaaccca gccgccagcg
     2101 catcgacttt acgctcttct gccagcgcca tgccaataaa gaacggtgcc attaaagaca
     2161 ttattcctaa tgttccgtta tatacgttgc cgccaatacc tttcagacca ttaagtgttt
     2221 caatggttga ggcgtcgagg cgaataccta aggaataaaa aacgacccct ccccaaagct
     2281 tagaaaaacg ttgttaatta atacaaacat ggcccccgca agggttaacg gcattaagcg
     2341 aataaagcca tttttgattg cattaacgtg tggctgcttt cctattttaa ctgcaaaagg
     2401 gaggagtacc ttttcaagcg atgcaataac attactcata gaaaaatacc cttaaaaacc
     2461 gcaatttaaa tattacggta ttgatttatg aaataactct ttgacgggaa aatttaaaat
     2521 aaattaattt gctgcggctt ttttaatcgc tgcaaccgca gccttaagca cgcctaaacc
     2581 atcgactttg ccataaagca gcgagtcaat tacttcaacc ggtttgttgg gtaacaaacg
     2641 ctggatttcg ggcaacatat aagcaatctg cggccctaat aacacgacat cggcattctg
     2701 acctttttca ccagccagtg tttccggaaa tgcttcaata atgaccggaa cttcatattt
     2761 ttctgcctgt gcgcgcattt ttgataccag taaagagata gacatgcccg cagaacaaaa
     2821 cagataaatg tgtttctttt ccataaaact gccctcatcg acgattatct gtcagccaga
     2881 cactccgcaa gccttgacct gcttccatgc tctgggtaac ttgcgaaacc aacatgactt
     2941 tttttgtggc tgaaaggagt atacggtata gacctgagca atagtatttc tttgacctta
     3001 taaattgttt ctccttgacc tgtggttatg accctcttca catttcgggc aaataattcg
     3061 cgaagataat taagaactca ggccaaaaaa aaccggcgca atggccggtt tccgttgtta
     3121 ctcaagcttt cagacgaatt gattacttcg cagcctgtgg atcagtgtcg tattcagcac
     3181 aggtctgata accggagttg atgacatgtc cggtatcatc taacgcgaca aagtaggttt
     3241 ctgctttacc atcacgttga cccaggatgt aggtctggca agtaccgcga gcatggatca
     3301 tgctcacttc agacgaaggt ttacccgcaa tttgtgcaac ctgcgcccgg ctcatgcctt
     3361 ttttgacgtc tttcaccaca ggctgtacaa actggtcttt ggtacgatca taagctgtac
     3421 aacccgccag catggttaat accgccgctg cactcagaat tcctgccata ttcttgttca
     3481 tattccgtcc tcttgtttat cagcgtgtta gataagcctg gaatacattg ggcgcttttt
     3541 caagcccgtg aacgaaacgg ctccgctttc agaggattcc tgtatgacgt tttaaccacc
     3601 attcagcccg ctgtcgcttg tcgtttcagt agcaacgggt tagctttaag gaagttttgt
     3661 cttttctgtc tggaggggtt caatgacatt gcaacaacaa ataataaagg cgctgggcgc
     3721 aaaaccgcag attaatgctg aagaggaaat tcgtcgtagt gtcgattttc tgaaaagcta
     3781 cctgcaaact tatccgttca ttaaatcact ggtgctcggg atcagcggcg gtcaggactc
     3841 cacgcttgcc ggaaagctgt gccagatggc gattaatgag ctgcgccagg aaactcgcaa
     3901 cgaatcactg caatttattg ccgtacgcct gccctatggt gttcaggctg acgaacaaga
     3961 ttgccaggat gccattgcct ttattcaacc ggatcgcgta ttaaccgtta atatcaaggg
     4021 cgcggtattg gctagcgagc aggcattgcg ggaagcaggc attgaactga gcgattttgt
     4081 ccgtggcaat gaaaaagcgc gtgagcggat gaaagcacaa tatagcattg cgggtatgac
     4141 cagcggtgtc gtggtgggca ccgatcatgc agcagaagcc attaccggat tcttcactaa
     4201 atatggtgac ggcggtacgg atattaatcc gctgtatcgt ctcaacaaac gccagggtaa
     4261 acagttactg acggcattag gttgcccgga acacctttat aagaaagccc caactgccga
     4321 tcttgaagat gatcgccctt ctctgccaga tgaagtggca ctcggcgtga cctatgacaa
     4381 tatcgacgac tatctggaag ggaaaaacct acctgaacag gtcgccagaa caatagagaa
     4441 ctggtatctg aaaaccgaac ataaacgccg tccgccaatt aacgttttcg atgatttctg
     4501 gaaaaagtaa taatttgcac ctattggatt gtgcgaaaaa gagtaatttg ttcacgccgg
     4561 atgcggcgtg aacgccttat tctacctaca aaactatgca aattcaatac attgcaggag
     4621 tcgaataggc ctgacaggcg tagcacgtca gacggtgtaa cctttgtcat caacccgcct
     4681 cttttttaat cgcctcccgc ctgttacact ggatagataa ccagcattcg gagtcaacag
     4741 tggtacggcg tttaacttct ccgcggctcg aatttgaagc tgcggcaatt tatgaatatc
     4801 ccgaacattt acattcattc cttaatgact tacccacccg accaggggtg tatctgtttc
     4861 atggcgaaag tgacaccatg ccgctctata tcggcaaaag cgttaatatc cgcagtcggg
     4921 tgctttctca tttacgcacc ccggatgaag ccgccatgct acggcaatcc cggcgaatta
     4981 gctggatctg caccgcaggc gaaatcggcg ctctgctcct tgaagcgcga ttaatcaaag
     5041 aacaacagcc gctgtttaat aaacggttgc gccgcaatcg ccagctctgt gccctgcaat
     5101 taaatgaaaa gcgcgtcgat gtggtgtatg ccaaagaggt ggatttttca cgagccccca
     5161 acctgtttgg cctgtttgcc aataggcgcg cagctttgca agcattgcag agcatcgctg
     5221 atgaacaaaa actttgttat ggcctgttgg gactggaacc gttaagtcgc ggtcgtgcat
     5281 gttttcgttc agcgctaaaa cgttgcgccg gagcatgctg cggtaaagag agccatgagg
     5341 aacatgcgct acgcttgcgc cagtctctgg agcgtttgcg agtggtgtgt tggccttggc
     5401 aaggtgcggt ggcactgaaa gaacagcacc cggaaatgac tcaatatcat attattcaaa
     5461 actggctgtg gctgggggcg gttaattcgc tggaagaagc gacaacgtta attcggacac
     5521 ccgccgggtt tgatcacgac ggttataaaa ttctttgtaa gccgctgctt tccggtaact
     5581 atgaaattac tgaacttgat ccggcgaatg accagcgagc cagttgattt cactgaacaa
     5641 cagtttacct tgcggttgta aaagccgcag ggtgtgcctg ccatcgaacc agcacacgcc
     5701 ctggtcggta gtgagcagtt tgtcgcccag ttgccacgcc ccgttaatca caaacaccac
     5761 cccgccgcgt gagccaaagg tagtaaacgt acgttcggca attctgactt tcgccttaca
     5821 gacatccagg cgcgtcataa tattgaaatc catcgacatc tgccctgccg tcagtttcgc
     5881 ttttaccacc tggtccgccg taaaggcaaa aggctgtaac ggttttaagg tatggttaaa
     5941 gcggtctgcg ctttcaagga gcatctcgcc gccttccagc aacgtcacta tcctttccat
     6001 gccgggaaac aaagaaaact ccccattcgc cgcgatggat gcaatgctgg cacgccagta
     6061 aaaatcacgt tttgccggtg ggaaagtgca aatttcgcgc gtttcaccgg cagcgtttcg
     6121 ccacagattc accgacattt tacgcatatc aaagtattcc atactcgctc ccggcccgtt
     6181 cttttcattg ttatcgttca ggtaaccgtg gatggttatc ggcaactcgg gtgatttacg
     6241 ttagtggtga tcaggctttt tttacaactg tttgagaaga gaaaagaaaa ccgccgatcc
     6301 tgtccaccgc attactgcaa ggtagtggac aagaccggcg gtcttaagtt ttttggctga
     6361 aagattattc agcagttgca ggcattttac cttttgccgc tggacgttct gtcagacgct
     6421 tctcaaaatt agcattaaat tgctttttct gttccggcgt caggatgttg taaattttgt
     6481 tctgggtttc catgtgcgcc agcatgttag ctttgcgctg ttcttccatt tttgcgatct
     6541 gcgcttcagc ttttgcttta tcgaaggtat cgctggcaat gatgtcatgc attgcgcggc
     6601 gttcttccag cggcggacgt ttcatctggt cacgctggcc tttcatgatt tcgtggatct
     6661 gctgtttctg cgcgtcggtc aggttcaggt ctttgaacat catgtcctga tgcggaccga
     6721 acttgccttt gtggtgcatc atcggcttcg cgtcagccgg tgctgcggta gtggtgtctg
     6781 cggcatgggc caggttagcc gcgccaagag ccagggtaga ggcaacaaac agtgcagtta
     6841 atttacgcat attctatatc cttcctttca gttatttatt acggctttct taagtagcgt
     6901 gccgtgttga cgagattaac tttactgact ttagcgtcaa ttaatcagag caacggtaaa
     6961 acaatgaaag tgtaaaaaac acttttgcgc caattatgga gaaaaaaaga aaatttgatg
     7021 gagagtgatg agagaatatt acaacacgat gattttgcag agattatgaa gaaccatacc
     7081 ggatgactgg tgataaataa agcaaataac caggattaat ctgtattaat ttataagaaa
     7141 gcaacttaat acccgcagaa tgatttctgc gggtaagtat tagcttattt tttcgagcat
     7201 taatcccgcg cgtaatccca acgctaccaa cggattaggg aataacacat actctacatc
     7261 atgggttacg gtaaaacgtt cctctccgtc ctgcgccagc aatgttcctt tctcaaacgg
     7321 cataaaattc agcgtgtcac ttgccatatg catttcgaag gacggcgagt ggcgagtaat
     7381 ttgcgaaacc acccgataac ggagcggcgg tgttctcacg ataccgacac tctcaccaga
     7441 tagcagcgca gcaattgcgc tggcagttac tgcaaactgg cgaagatcgt tttgcccaaa
     7501 gggcaacgct ttgccaagtt ccaacgtaca ggccagcgcg ccaaaatgtc tgacgctgaa
     7561 atgggtaaac gtaccaccag gctcctgatg gaacaccagc gcctccagcc ccgccgcacc
     7621 cagccacgtc agaaatttct cgtcccaggg aatgtcgcgt tgcggtaata caccgaactg
     7681 cggatgcaag gagccacgaa ttgcggtatg tagatcaagg tgccaacgca cagattcttt
     7741 gccctggtca taaaaatctt ccaggcactg ttccagttcg cgcgcccgac aggtttctcc
     7801 gctttcagca aatagctgcc aacgaccgcc aaacattcgg ttcatatcgc tatggcaata
     7861 acgtttccct tgcttcagcg caggaggatt cccgaggatc accagcaacc gccaacgtaa
     7921 cgggatctcg ccgtgagata tcgcgccaag caacgcgtcc agcatttcca caggtgccgt
     7981 ctcattaccg tggatccccg cagaaatcac cagcgcgcct tgcggtggcg ttaatggcgt
     8041 cagttccagc acaccatcgc ccagccagcg ccagcgaacg ccgttgattt ctcgctcggt
     8101 gataaccggt tttttacccg ttaaggtcag agcaagaaaa ttatccattg ccccctccct
     8161 cgcgctggaa cggataaacc gaaccgagat tcagtaattg gctcaataca tccagtgctt
     8221 cccgcccttc gcgcagcaat tgcgggtcgg ccagatcggc agcagtaaga cgatcgcggt
     8281 agtaacgatc cacccagtca ttgagcgcat taaacaaggt atcgttcatc atcaccgccg
     8341 gattcaccgc ccggcgttct tcttctgtca ataccacccg caaccgcagg cacgccgggc
     8401 cgccgccatt cgccatgctt tcacgtaagt caaagacttt tagttcgcta atcgggttgt
     8461 cagcggcaag gagttcattg agataacccc atactccggc gtgttcccga cactcctgag
     8521 gcagcaccaa catcatggaa ccatcatcgc ggctcaacag ttggctgtta aacagatagg
     8581 tagacaccgc atccgacacg gaaacctgag ttgccggaac ttctatcgcc ataaaaccgt
     8641 tcacccgcgt gccagtaact gtgactggcg agcgaacgcc tgttggtggc aaaacagcac
     8701 ctggcggtta ctcacggcaa tcacgtcatt atgaaaaacg ctctggtcga taacgtccgg
     8761 gttttgctgg gcgaaaatca cctgttgggg attcacctga ttaagccttg ccaccgcctc
     8821 gctggcttcg cgagtctgtc gcgccggata acgggaaggc cgggtatcat tgccttcttc
     8881 tcgcccgtag acaaaaagta gcatacccgg ttcaccgtaa tgaccgccga gacgattgtg
     8941 gtttgccgcc ccctcatcac cgagcaacgc tacctgcggc aacgccgaat ggacgctaaa
     9001 tttctcttcg tccttaaaaa tcgcttttaa cagcgattca gtgacgggcg cttccagcga
     9061 acggtgaaat ttattgttca ggttggcaac ggtgagatgc actttgccat ccagcgtatc
     9121 ggcagatggc gcgatcgttg ccgcattggc tacccacatt ggcgaagcgg aactgacgct
     9181 ggaaagccag tgcggtgcct ggcgtgcaac tttttccagt acctgctcat cgctaccact
     9241 gaatcccaac tgacgcagca ccggaataaa cggacgctcg tgcggcggaa tcacggcctg
     9301 ggggaatccc gcatcggcaa gggctttcat tttcagtaag ccctgttttg ccgccagtcg
     9361 cgggttagac acctgaaaac ggtgacgggt agaggcttca ttaccaaacg acaggcccgc
     9421 gtaatgatgc gtcagcccta ccagcccgtc gaaattaact tcccaggcgt tcatcgcacc
     9481 acctcatcgg aaaaatccag cccggggtta agcgtggcgg gcaatgttaa cgagtccgac
     9541 tccaggctcg ccataggcca tgcgcagtaa tctgcggcat accaggcgct ggggcgatgg
     9601 ttaccggaag caccaatgcc gccgaatggg gcggtactgg cagcaccagt aagcggtttg
     9661 ttccagttaa caatccccgc ccgcgcctcc agcaacagtt gatcgaattt ttcccgctcg
     9721 ggggaaacca gaccgcaaaa gagtccgaag cgagtgttgt tcgccatccg aatcgcctca
     9781 tcaaaagtat cataacgcca gacgcgcaaa aacggcccga acacctcttc atccggcaca
     9841 ccgccaacgc ccgtcatttc aatgatccct ggtgtcagca acgatgtccc tgcttgtaat
     9901 aagcgcggcg caagcagggt tcgtccgccc atcgcttcca gttgctgcca ggcagtaacc
     9961 acctgctgtg cggcctgttc agaaatcagc ccgccaataa acggctgcgg ttcgtcatcc
    10021 cagttgcccg gcgttaatcg ctggctgacg gcaaccagac tagcaagaaa cgcatcgccc
    10081 tgcgctccgc ttttgagcaa taaacggcgg gcgcaggtgc agcgttgccc ggctgtgaca
    10141 aacgccgact gaatggtcag atggacagcc gcgtcgatat ccgccacctc atcgataatt
    10201 agcggattat taccgcccat ctcaagggcg agaattttct ccggctgacc ggagagctgg
    10261 cgatgcagct ggtagccggt attggcgcta ccggtaaaca gcaaaccgtc gagatcctcc
    10321 agcgcactca gcgcctgacc cgtttcacgc ccgccctgca ccaggttcag cacgcccggc
    10381 ggcaagccag cctgctgcca taaacgcatt accgcttcgc cactccacgg tgtcagttcg
    10441 ctgggtttaa agataatggt gttacctgcc agcaatgccg gaacgatatg tccgttcggc
    10501 aaatgaccag ggaaattata cggcccaaac accgccagca cgccgtgcgg gcgatgtcgc
    10561 aggctcgccg caccgtccgg catttcacta cgctgctcgc cggtacgaac gtgatacgcc
    10621 ttaattgata tcgcgatttt attgatcatc gccgtcactt cggttgccgc ttcccagcgc
    10681 ggcttacccg tttctctggc aataatcgcg gttaattcgg ctttattgct ttccagcaat
    10741 ccggcaaagc gttcgacaac gacctgacgt tcagccaatg agagccgcgc ccagcgcgga
    10801 aacgccgcac gggctgcccg acaagcctgt tcgacctgag cggcatcggc atcattgcct
    10861 tgccataaca cctcgcccga taccggatta cgcttcacac gcgatgcgcc ctggcccgtt
    10921 atccagtcac cgttaatcca taaagtcatg ctgttttctc ctctgcgcac aggcgcacca
    10981 gacgaacgcg atcccctgcg tggcatttga gggcatccag ttgtgcggcg gttaaaatca
    11041 aacgctcggt tgccggatcg gtacgcgcca gcaccacgcg gaaatggtga taattttcat
    11101 tggcgaccag gcaggccgga aaatcgccct gcgcaggctg cccttctgcc acttccacca
    11161 gccgactttt acggatggcg cgcacgcggt cgatgtcaca ctcaagcgtt ggcccgccgt
    11221 cgaagatgtc gatatagttg cggtaacgaa aaccttcttt ctccagcacc gcgcgggcag
    11281 gcgcggtttg cggatgtacc tgaccgatga cgtcctgggc ttcctgggat aaaaagtggg
    11341 tatagatcgg atgtttcggc atcagttctg caataaatgc cttttgcccg gtgccgcaga
    11401 gaaaatcggc gcggctaaaa tccatcgaaa agaagcgttt accgaggctt tgccagaacg
    11461 gtgaatagcc gtgttcgtca atcaccccgc gcatttcagc aaccactttg tcattaaact
    11521 tgtcgcgaaa agccgccata aacataaagc gcgatttcga tagcaaatag ccgttgccct
    11581 ctttgcgcca gtccgggtcg agaaacagcg tgcacagctc gctgctgccg gtgtgatcgt
    11641 tactaagaaa cagcgtcggc aatgcgttat agacattcag ctcttttgag gcgtgaacca
    11701 acgtgccgac gcgatagttg taccagggat cgttcagccc aaccgccacc tcaatagcgc
    11761 aaatccccgc cacggtgcct gtctcgctat cttccagcac gaacacatag ccctgctcgc
    11821 ttttgggcag ttcaccttgc caggttttga ttgccctttc gatacgcgcc gaaagcgtgg
    11881 cttcattggc gggaagcgac gtcaggccgc cgcccgtttt gctggcaagc tgcatcagcg
    11941 ccgagacatc tgagcgctca acgggacgga tgaccatcat gatgaacctc ggctaacaaa
    12001 gtgttcgcaa gcagctgcaa agcgatccag tccggtcgtc acctcttctt cgctgacatt
    12061 gagcgcaggc gcaaaacgca ccacgttgcc acccgcaatc agtaccatca cgcctgcttt
    12121 cgccgcttcc agagagatct gtttcgcttg cccggcgtaa tcggcattca gtacacagcc
    12181 aatcaacaaa cctaagccgc gaacttcact gaacaaacca tagcggtgat taatagtatt
    12241 aagacgctca acaaaccagt cgtgacgctg tttaacgcca ttaagcatct ctggtgtgtt
    12301 gatgagctcc agcactttgc ctgctaccgc cgaggccagc ggattaccgc cataggtggt
    12361 gccatgagtg ccaacggtca tcacgcgggc gcactcttcg gttgccaaca acgcaccgac
    12421 cgggaaaccg ccgcccagcg ctttggcggt ggttaacaga tcgggcgtca cgccgtagtg
    12481 catataggca tacagctcac cagtgcgccc gacgccggtt tgtacctcat caaaaatcag
    12541 caaggcattg tggcggtcac acaattcacg cagaccttgt aaaaacgcgt tgctggctgg
    12601 caccacaccg ccttcgccct agatgggctc gacaatcacc gcacaggtgg cgtcgtcaat
    12661 cagcgcgctg gcagagttaa tatcgttata tgcagcatga cgaatatccg ccggcagtgg
    12721 cgcaaaatcc tgtgaatagg ctggctgccc acccgcactg acagtaaaca gcgtgcgacc
    12781 atgaaacgca tttttgaacg ccacgatgcc actcttatgg ctgccgtagc ggtcgtgagc
    12841 gaatttacgc gccagtttta gcgccgcttc gttggcttcc gcaccggagt tacaaaagaa
    12901 gacgcgatcg gcaaacgtgg cgtcgatcaa ttttttcgcc agtcgcagta ccggctcgtt
    12961 ggtgtaaccg ttgccggtat gccagaactt actcgcctgt tcgttcagcg cttcacgcag
    13021 ttccggatgc gcatggccca gcgcgttcac cgcaatgcca cccgcgaagt cgatatactc
    13081 tttcccctgc tgatcccaca agcgcgaacc ttcgccacgt accggtataa agggtgccgg
    13141 agcgtaaaca ggtatcatcc attcatcaaa gttttcacgc gtaattggct gagacatagc
    13201 gacctctaca gtaaataatt cgttatttgt atgttaacaa taagtaatgt ttgcgctgta
    13261 aatgtagatt gcagggttcg tgccagccag tgataaaagt gcataaacgg cggaggctaa
    13321 ctggaaatca aggagttata accaaaccat atgcatttaa agtgcatata aagtgaatac
    13381 gtttgcgatg tgggtgaata aaaagaataa aaaacgcaat gttatgcaga agtaaaatat
    13441 aattctagaa ttgtgatcat tgacgaaatt tactggaaat tactgcgcca ttctgacgca
    13501 gcgcgcacca aaagcgggca ttttttgcgc catcgttgac atcattaaca accatcgatc
    13561 aaatcactta acaacaggcg gtaagcaacg cgaaattctg ctaccatcca cgcactcttt
    13621 atctgaataa atggcagcga ctatgaaatt tgtctctttt aatatcaacg gcctgcgcgc
    13681 cagacctcac cagcttgaag ccatcgtcga aaagcaccaa ccggatgtga ttggcctgca
    13741 ggagacaaaa gttcatgacg atatgtttcc gctcgaagag gtggcgaagc tcggctacaa
    13801 cgtgttttat cacgggcaga aaggccatta tggcgtggcg ctgctgacca aagagacgcc
    13861 gattgccgtg cgtcgcggct ttcccggtga cgacgaagag gcgcagcggc ggattattat
    13921 ggcggaaatc ccctcactgc tgggtaatgt caccgtgatc aacggttact tcccgcaggg
    13981 tgaaagccgc gaccatccga taaaattccc ggcaaaagcg cagttttatc agaatctgca
    14041 aaactacctg gaaaccgaac tcaaacgtga taatccggta ctgattatgg gcgatatgaa
    14101 tatcagccct acagatctgg atatcggcat tggcgaagaa aaccgtaagc gctggctgcg
    14161 taccggtaaa tgctctttcc tgccggaaga gcgcgaatgg atggacaggc tgatgagctg
    14221 gggggtggtc gataccttcc gccatgctaa tccgcaaaca gcagatcgtt tctcatggtt
    14281 tgattaccgc tcaaaaggtt ttgacgataa ccgtggtctg cgcatcgacc tgctgctcgc
    14341 cagccaaccg ctggcagaat gttgcgtaga aaccggcatc gactatgaaa ttcgcagcat
    14401 ggaaaaaccg tccgatcacg cccccgtctg ggcgaccttc cgccgctaat ttagcagctc
    14461 tcctggctca aactgggtca ggagaattaa ccttgagaaa aatcaacaaa ctgtcagtaa
    14521 tgatttgttg cctgccgtcc tttgttatac cgtcactgcg tttttagttg tctgaccact
    14581 tctctattat caagtttgat ataggaaact ccacgatgaa cgctgagcgt aactttctgt
    14641 ttgcctgtct tatttttgcg ctggtcattt acgctatcca cgctttcggt ttattcgatc
    14701 tgctcaccga tttaccccac ttacagacac tcattcgcca gagcggattg ttcggttata
    14761 gcctctatat tctgttattc atcaatgccc ccctctttct gttaccagga agcatactgg
    14821 tgatcgctgg aggaatagtt tttggcccgc tcttagggac acaactctca ttaattgccg
    14881 caacgctggc ctcctcgtgc tcattcctgc tggcgcgctg gctgggacgt gatttactgc
    14941 tgaaatacgt gggccatagc cataccttcc aggccattga aaaaggcatt gcgcgtaacg
    15001 gtattgattt tcttattctg acccgcttaa tcccgttgtt tccttacaat attcaaaatt
    15061 acgcttacgg attcaccacc atcgccttct ggccttatac ccttatttcg gcactcacga
    15121 ccctgcccgg tattgttatt tataccgtga tggcaagcga tctcgccaat gaaggcatta
    15181 cgctgcgctt tattttacaa ctctgtctgg cgggcctggc gctgtttatt ctcatccagc
    15241 tcgcaaaact ctacgcccga cacaaacatg tggatctgtc tgcttcgcgc cgcagcccac
    15301 ttactcaccc aaaaaatgaa ggatagaacg atgtcgcaac attattcagt gtcatggaaa
    15361 aaaggactgg ctgcactctg cttactggct gttgccgggc ttagcggctg cgatcaaaaa
    15421 gagaatgcgg cggcaaaagt ggaatacgac ggactttcga acagccaacc gctgcgcgtc
    15481 gatgccaata accatacggt aaccatgctg gtgcaaatta atggtcgttt cctcaccgac
    15541 gacactcgtc acggtattgt gtttaaagat ggctccaacg gacataaatc gctgtttatg
    15601 gcttatgcgc ccccgaaagc attttatgaa gccctgaaag aggcaggtgg tacgccgggc
    15661 gaaaacatga cgatggataa taaagaaacg actcatgtca caggcagcaa actggatatt
    15721 tcggtcaact ggcaaggggc ggcaaaagcg tattccttcg atgaagtgat tgttgacagt
    15781 aatggcaaga aactggacat gcgctttggc ggtaatttaa cggcagcaga agagaagaaa
    15841 acgggttgcc tggtgtgtct ggatagctgc ccggtcggca tcgtcagcaa tgcaacatac
    15901 acttatggtg cggtggaaaa acacggcgaa gtgaaattca aaggcaatgc ctcagttctc
    15961 ccggcggata acacgctggc aacggttacc tttaaaatca ccgaataaag ccaagataaa
    16021 ggatgatgaa gatacaatcg cgtaaaatct ggtactaccg tataaccctc atcatcctgt
    16081 tgttcgctat gctgctcgca tgggcgctgc ttcccggcgt ccatgagttt atcaatcgca
    16141 gcgttgcggc gtttgccgcc gtggaccaac agggtataga acgctttatt cagtcttacg
    16201 gcgcactggc ggcagttgtc tcgttcttgt tgatgatttt acaggccatt gctgcaccgc
    16261 tacctgcgtt tttgatcacc tttgccaatg cgtcgctgtt tggcgcgttc tggggcggct
    16321 tgctgtcgtg gaccagttcg atggccggcg cggcgctgtg cttttttatc gccagagtga
    16381 tgggccgcga agtggtggaa aaattaaccg gcaaaaccgt gcttgacagt atggacggct
    16441 ttttcactcg ctacggcaaa cacaccatcc tggtctgtcg gttattgcct tttgtccctt
    16501 tcgatccaat cagctatgct gccggtctga cttcaatacg ttttcgctcg ttttttatcg
    16561 ccaccgggct tggtcaatta ccggcgacta ttgtttattc ctgggcaggc agcatgttaa
    16621 caggcggtac tttctggttt gtcaccgaac tgtttattct gtttgccctg accgtggtga
    16681 tttttatggc gaagaaaata tggcttgagc gccagaagag gaatgactga tgggtttacc
    16741 gccgcttagc aaaattcctt tcattttacg tccgcaggcg tggctgcatc gtcgccatta
    16801 cggcgaggtg ctaagcccga ttcgctggtg ggggcggatc ccgtttatct tttatttggt
    16861 gtcaatgttt gttgggtggc tggagcgcaa acgctcaccg ctcgatccgg tagtgcgatc
    16921 gcttgtcagc gcgcgcattg cgcaaatgtg cctgtgtgag ttttgcgtgg atatcaccag
    16981 tatgaaagtc gccgagcgta ccggcagcac cgataaactg ctggcagtgg ctgactggcg
    17041 gcaaagcccg ctctttagcg atgaagaacg gctggcgctg gagtacgccg aagccgcaag
    17101 cgtcacaccg ccaacagtcg atgatgccct gcgtacccga ctggctgcgc attttgacgc
    17161 tcaggcgctc accgaactga cggcattgat cggcctgcaa aacctgtcgg ctcgttttaa
    17221 ttctgccatg gacattcccg ctcaggggct gtgccgtatt ccggaaaaac gttcttaagg
    17281 agggaagatg cgccattgtg ggtggttgct gggattgtta tcgctgtttt ctttggcaac
    17341 acatgccagt gactggcagg aaattaaaaa tgaggccaaa gggcaaaccg tctggtttaa
    17401 cgcctggggc ggcgataccg caattaaccg ctatctcgac tgggttagcg gcgagatgaa
    17461 aacccattac gctataaacc tgaagattgt tcgtctcgcg gatgccgcag acgcggtgaa
    17521 gcgcattcag accgaagctg ctgccggtcg taaaacgggc ggctcggtgg atctgctctg
    17581 ggtgaacggc gaaaacttcc gcaccttaaa agagtaatgc cagtcagtta agcaactgac
    17641 tggcatt
//
LOCUS       DAETQL010000080        17391 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.080, whole genome shotgun sequence.
ACCESSION   DAETQL010000080 DAETQL010000000
VERSION     DAETQL010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.080"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            251..1276
                     /gene="purR"
                     /locus_tag="K0W65_003018"
     CDS             251..1276
                     /gene="purR"
                     /locus_tag="K0W65_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBI3096194.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(1273..2205)
                     /locus_tag="K0W65_003019"
     CDS             complement(1273..2205)
                     /locus_tag="K0W65_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3096195.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            2318..3529
                     /locus_tag="K0W65_003020"
     CDS             2318..3529
                     /locus_tag="K0W65_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBI3096196.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            3820..4968
                     /gene="cfa"
                     /locus_tag="K0W65_003021"
     CDS             3820..4968
                     /gene="cfa"
                     /locus_tag="K0W65_003021"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBI3096197.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(5008..5649)
                     /gene="ribE"
                     /locus_tag="K0W65_003022"
     CDS             complement(5008..5649)
                     /gene="ribE"
                     /locus_tag="K0W65_003022"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBI3096198.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            5864..7237
                     /gene="mdtK"
                     /locus_tag="K0W65_003023"
     CDS             5864..7237
                     /gene="mdtK"
                     /locus_tag="K0W65_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBI3096199.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(7278..8534)
                     /locus_tag="K0W65_003024"
     CDS             complement(7278..8534)
                     /locus_tag="K0W65_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBI3096200.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            8842..8918
                     /locus_tag="K0W65_003025"
     tRNA            8842..8918
                     /locus_tag="K0W65_003025"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8876..8878,aa:Val,seq:gac)
     gene            8923..8999
                     /locus_tag="K0W65_003026"
     tRNA            8923..8999
                     /locus_tag="K0W65_003026"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8957..8959,aa:Val,seq:gac)
     gene            9107..9412
                     /locus_tag="K0W65_003027"
     CDS             9107..9412
                     /locus_tag="K0W65_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBI3096201.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            9538..10960
                     /locus_tag="K0W65_003028"
                     /pseudo
     CDS             9538..10960
                     /locus_tag="K0W65_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(10972..11784)
                     /locus_tag="K0W65_003029"
     CDS             complement(10972..11784)
                     /locus_tag="K0W65_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096202.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(11788..12573)
                     /locus_tag="K0W65_003030"
     CDS             complement(11788..12573)
                     /locus_tag="K0W65_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBI3096203.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(12570..13238)
                     /locus_tag="K0W65_003031"
     CDS             complement(12570..13238)
                     /locus_tag="K0W65_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBI3096204.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(13302..13940)
                     /locus_tag="K0W65_003032"
     CDS             complement(13302..13940)
                     /locus_tag="K0W65_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HBI3096205.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(13953..16054)
                     /locus_tag="K0W65_003033"
                     /pseudo
     CDS             complement(13953..16054)
                     /locus_tag="K0W65_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(16075..16701)
                     /locus_tag="K0W65_003034"
     CDS             complement(16075..16701)
                     /locus_tag="K0W65_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HBI3096206.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(17157..17366)
                     /gene="fumD"
                     /locus_tag="K0W65_003035"
     CDS             complement(17157..17366)
                     /gene="fumD"
                     /locus_tag="K0W65_003035"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HBI3096207.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         4229 a         4501 c         4064 g         4597 t
ORIGIN      
        1 tttacacact gtgatgaaaa aatctcccgt catttataat gataagtgtt tttaccactt
       61 ccccttttcg tcaagatcgg ccaaaattcc acgcttacac tatttgcgta ctggccattg
      121 accccttcct gacgctccgt gtcgtttttc cggcgtaccg caacactttt gttgtgcgta
      181 aggtgtgtaa aggcaaacgt ttaccttgcg attttgcagg agctgaagtt agggtctgga
      241 gtgaaatgga atggcaacaa taaaagatgt agcgaaacga gcaaacgttt ccactacaac
      301 tgtgtcacac gtgatcaaca aaacacgttt cgtcgctgaa gaaacgcgca acgccgtgtg
      361 ggcagcgatt aaagaattac actactcccc tagcgcggtg gcgcgtagcc tgaaggttaa
      421 ccacaccaag tctatcggtt tgctggcgac cagcagcgaa gcggcctatt ttgccgagat
      481 cattgaagca gttgaaaaaa attgcttcca gaaaggttac accctgattc tgggtaatgc
      541 gtggaacaat cttgagaaac agcgggctta tctgtcgatg atggcgcaaa aacgcgtcga
      601 tggtctgctg gtgatgtgtt ctgagtaccc agagccgttg ctggcgatgc tggaagagta
      661 tcgccatatc ccaatggtgg tgatggactg gggtgaagca aaagctgact tcaccgatgc
      721 ggtcattgat aacgcgttcg aaggcggcta catggccggg cgttatctga ttgaacgcgg
      781 tcaccgcgaa atcggcgtca tccccggccc gctggaacgt aacaccggcg caggccgcct
      841 tgccggtttt atgaaggcga tggaagaagc gatgatcaag gtgccggaaa gctggattgt
      901 gcagggtgac tttgaacctg aatccggtta tcgcgccatg cagcaaatcc tgtcgcagcc
      961 gcatcgccct actgccgtct tctgtggtgg cgatatcatg gcaatgggcg cactttgtgc
     1021 tgctgatgaa atgggcctgc gcgtcccgca ggatgtttcg ctgatcggtt atgataacgt
     1081 gcgcaacgcg cgctatttta cgccggcgct gaccacgatc catcagccaa aagattcgct
     1141 gggtgaaaca gcgttcaaca tgctgttgga tcgtatcgtc aacaaacgtg aagaaccgca
     1201 gtccattgaa gtgcatccgc gtttgattga acgccgctcc gtggctgacg gcccgttccg
     1261 cgactatcgt cgttaatcac ccgttgcggg agtctcttcc ggctcccgca gccactcctt
     1321 attcagcgtc tcactatcgc tgagatactc aagcagccag gttaacgcag gcgacatatc
     1381 attttgctgc catgtcagac aacatgccga atccgggaag gggttttcca gttcaagtgc
     1441 tacccacttc ccctcattaa gccacggttt ggcgaaatgt gttggcacca tccctatgca
     1501 taatcctgcc gagatacagg ttgccgatga ttcccagtca ggtacgacaa gtcttttttg
     1561 gttatccagc aaccaggtaa tacgtttagg tagcgttcgc gaggtgtctt cgcgcaccaa
     1621 cgacggccag ttgcgcaacg tatcatcgct gaacgggcca tccatcaacg ccagcgggtg
     1681 gtgactggca acaacgcaac tccagcttag catccccata tcccggaagg cataacgacc
     1741 gcctaccgga atcgcgcgtg ttgcgccaat tgccagttcc acgcgcccgt cggaaagcgc
     1801 atcccagaca ccgttgaaca cttcctgaaa gacaagaagt tcgacatcat caaaatggcg
     1861 ataaaaatca acgatcatct gccgtgtacg ttctggcctg acaatattat ccactgcgat
     1921 agctaactga ccgcgccagc cgttcgctat ctgctgacat tgctggcggg tgatctgcat
     1981 ttttttgaca acagagcgcc cttctttgag aaaccacgct ccagcagcgg tcagctccac
     2041 atcacggtgc cgtcgttcaa agagcggtac cgccagccac tcttccagct gacgcacggt
     2101 atagctgacc gcagaaggaa cacgatgcag ctcctgtgcc gcagcgctaa aactaccatt
     2161 acgcgctacc gcatcaacaa cttcgagtga atattctgac cacatagtct gcctgcaaaa
     2221 tttttgaaac cagtcatcaa atattaccgt ttcacaacac taatttcact ccctacactt
     2281 tgcggcggtg tttaattgag agatttagag aatatacatg caacctggga aaagattttt
     2341 agtctggctg gcgggtttga gcgtactcgg ttttctggca accgatatgt atctgcctgc
     2401 tttcgccgcc atacaggccg acctgcaaac gcctgcgtct gctgtcagtg ccagccttag
     2461 tctgttcctt gccggttttg ccgcagccca gcttctgtgg gggccactct ccgaccgtta
     2521 tggtcgtaaa ccggtgttat taatcggcct gacaattttt gcgttaggta gtctggggat
     2581 gctgtgggta gaaaacgccg ccacgctgct ggtattgcgt tttgtacagg ctgtgggggt
     2641 ctgcgccgcg gcggttatct ggcaagcatt agtgacagat tattatcctt cacagaaagt
     2701 caaccgtatt tttgcgacca tcatgccgct ggtgggtctg tctccggcac tggctcccct
     2761 gttaggaagc tggctgctgg tccatttttc ctggcaggcg attttcgcca ccctgtttgc
     2821 cattaccgtg gtgctgattc tgcctatttt ctggctcaaa cccacgacga aggcccgtaa
     2881 caatagtcag gatggtctga cctttaccga cctgctacgt tctaaaacct atcgcggcaa
     2941 cgtgctgata tacgcagcct gttcagccag tttttttgca tggctgaccg gttcaccgtt
     3001 catccttagt gaaatgggct acagcccggc agttattggt ttaagttatg tcccgcaaac
     3061 tatcgcgttt ctgattggtg gttatggctg tcgcgccgca ctgcagaaat ggcaaggcaa
     3121 gcagttatta ccgtggttgc tggtgctgtt tgctgtcagc gtcattgcga cctgggctgc
     3181 gggcttcatt agccatgtgt cgctggtcga aatcctgatc ccattctgtg tgatggcgat
     3241 tgccaatggc gcgatctacc ctattgttgt cgcccaggcg ctgcgtccct tcccacatgc
     3301 aaccggtcgc gccgcagcgt tgcagaacac tctgcaactg ggtctgtgct tcctcgcaag
     3361 tctggtagtt tcctggctta ttagtatcag cacgccattg ctcaccacca ccagcgtgat
     3421 gttatcaaca gtagtgctgg tcgcgctggg ttacatgatg caacgttgtg aagaagttga
     3481 ctgcccgaat catggcaatg ccgaagtcgc tcatagcgaa tcacactgat ctatatcgat
     3541 atacttatac ttaggctgct aacaaaattt tgttgtatga ttgaaattag cggcctatac
     3601 taatttcgag ttgttaaagc tacgataaat attatgtttt tacggggaca ggatcgttcc
     3661 cgactcacta tggatggtca tttcggcaag ggttcctcct ttccctctgt tctacgtcgg
     3721 attatagact cgcggttttt tctgcgagat ttctcacaaa gcccaaaaag cgtctacgct
     3781 gttttaaggt tctgatcacc gaccagtgat ggagaaacta tgagttcatc gtgtatagaa
     3841 gaagtcagtg taccggatga caactggtac cgtatcgcca acgaattact tagccgtgcc
     3901 ggtatagcca ttaacggttc tgccccggcg gatattcgtg tgaaaaaccc cgattttttt
     3961 aaacgcgttc tgcaggaagg ctctttgggg ttaggcgaaa gttatatgga tggctggtgg
     4021 gaatgtgacc gactggatat gttttttagc aaagtcttac gcgcaggtct cgagaaccaa
     4081 ctcccccatc atttcaaaga cacgctgcgt attgccggcg ctcgtctctt caatctgcag
     4141 agtaaaaaac gtgcctggat agtcggcaag gagcattacg atttgggtaa tgacttgttc
     4201 agccgcatgc ttgatccctt catgcaatat tcctgcgctt actggaaaga tgccgataat
     4261 ctggaatctg cccagcaggc gaagctcaaa atgatttgtg aaaaattgca gttaaaacca
     4321 gggatgcgcg tactggatat tggctgcggc tggggcggac tagcgcacta catggcatct
     4381 aactatgacg taagcgtggt cggcgtcacc atttctgccg aacagcaaaa aatggctcag
     4441 gaacgctgta aaggcctgga tgtcaccatt ttgctgcaag attatcgtga cctgaacgac
     4501 cagtttgatc gtattgtttc tgtggggatg ttcgagcacg tcggaccgaa aaattacgat
     4561 acctattttg cggtggtgga tcgtaatttg aaaccggaag gtatattcct gctccatact
     4621 atcggttcga aaaaaaccga tctgaatgtt gatccctgga ttaataaata tatttttccg
     4681 aacggttgcc tgccctctgt acgccagatt gctcaatcca gcgaacccca ctttgtgatg
     4741 gaagactggc ataacttcgg tgctgattac gatactacgt tgatggcgtg gtatgaacga
     4801 ttcctcgccg catggccaga aattgcggat aactacagtg aacgctttaa acggatgttt
     4861 acctattatc tgaatgcctg cgcaggtgct ttccgcgccc gtgatattca gctctggcag
     4921 gtggtgttct cacgcggtgt tgaaaacggc cttcgagtcg ctcgctaaag gctattctat
     4981 cgccccctct tcgggggcga gttcagatca ggcttctgta cctggttgat tcatggcatt
     5041 ttctcgtgcc gccagcacac gttctaccgt atctaccact gcctgagttt gtggatcgat
     5101 ttcaatgttg acgcgtgcgc caagtttttt cttcccaaga gtcgtgcgtt ccagtgtttc
     5161 cggaattaaa tggacgcaaa aacgcgttgg cgtgacttcg ccgacggtca ggctaatacc
     5221 gtcgatgcca ataaatcctt tgtacaggat atatttcatc aactgactat cctggacttt
     5281 aaaccagatc tggcgattat tttccgaggt taatattttc gccacttcag cggtggtcat
     5341 aatatgacct gacattaagt gtccgccaat ttcatcactg aatttcgccg cacgctcaac
     5401 gtttacccaa tcccccactt ttaaatcgcc aagattggta atgcgtaacg tttctttcat
     5461 caggtcaaaa ctgacatggt tgccgttaat ttccgtcacg gtcaggcagc aaccgttatg
     5521 cgccacggaa gcaccggttt ccaggccgtc cagcatgtgg tcgggtaact ccaccacatg
     5581 cgtacgaaaa tttggtttct cgtcaatcga caccagtttt gcggtgccct gtacaatccc
     5641 cgtaaacata cttacaactc ctgaaatcag ttaagacatt ctgttcagca caatagcagg
     5701 tggaaaacgc ccttaccagt gaaggggtaa gaatggctat tttttcactg gagaattaat
     5761 aaatcctcgc tacaatagac tgaatttccc ctgcttcttc tttttgctgc ccattcaggc
     5821 ggctttttag tctctcatat aactacaaat aaaaggtgtt cacgtgcaga agtatatcag
     5881 tgaagcgcgt ctgttattag cattagcaat cccggtgatt ctcgcgcaaa tcgcccaaac
     5941 tgcgatgggt tttgtcgata ccgtgatggc gggcggctat agtgccaccg acatggcggc
     6001 ggtcgctatc ggtacttcta tctggcttcc ggcgatcctc tttggtcacg gactgctgct
     6061 ggcattaacg ccggttatcg cgcaattaaa tggttccggt cgacgtgagc gcattgcgca
     6121 tcaggtgcga caaggtttct ggctggcagg ttttgtttcc gttctcatta tgctggtgct
     6181 gtggaatgca ggttacatta tccgctacat ggaaaacatc gatccggctc tggcggacaa
     6241 agccgtgggt tatctgcgtg cgttgttgtg gggcgcgccg ggatatctgt tcttccaggt
     6301 tgcccgtaac caatgtgaag gtctggcaaa aaccaagccg ggtatggtaa tgggctttat
     6361 cggcctgctg gtgaacatcc cggtgaacta tatctttatt tatggtcatt tcggtatgcc
     6421 tgagctcggt ggcgttggtt gtggcgtggc tactgcggcg gtgtattggg tcatgttcct
     6481 tgccatggtt tcttacatta aacgcgcccg ctccatgcgc gatattcgta acgaaaaagg
     6541 caccgctaag cccgagcctg cggttatgaa acgactgatt caactcggtt tgccgattgc
     6601 gctggcactg ttctttgaag tgacactgtt tgccgtcgtg gctctgttag tgtctccgct
     6661 cggtattgtt gatgtcgcag gacaccagat tgccctgaac tttagttcac tgatgtttgt
     6721 gcttccaatg tcgctggcgg cagcggtaac tatccgcgta ggttatcgtc tgggtcaggg
     6781 ctcaacgctg gatgcgcaaa ccgctgcgcg gaccgggctt atggtgggtg tctgtatggc
     6841 aaccctgacg gccattttca cggtttcact gcgggagcaa atcgccctgt tgtacaacga
     6901 caatcccgag gttgtaacgc tggctgcgca tttgatgttg ctggcggcgg tatatcagat
     6961 ttctgactca atccaggtga ttggcagtgg gattttgcgt ggttataaag atacgcgttc
     7021 cattttctat atcaccttta cagcttactg ggtgctgggc ttgccaagcg gctatattct
     7081 ggcactgacc gatctggtcg ttgaacctat ggggccagca ggcttctgga taggctttat
     7141 tatcggcctg acatcggcag ccattatgat gatgctgcgt atgcggttcc tgcaacgtat
     7201 gccgtcagcc atcattctgc aacgagcgtc ccgctaataa agacaaggcg caaccttcac
     7261 gggttgcgcc tgtgttttta cgcaggctgg agcgttgcgc caatcccgtc ttcgtctggc
     7321 tgtaatttca gagcgttaca cagagtttgc cgactggcat ctgttatctc aacgcgtata
     7381 tccccgttaa cttccccttt cacattgatg aagtcaggtg aaatttcttt cgcactggtt
     7441 tgcaggcaaa tcgctccctc tccgcgcagg cgtttcactt tcagttgcac atgcgttgcc
     7501 gcaccagtga tcagccacag ttttccgcca ttcagattga tgttaccaac aatgaaagtg
     7561 acgctgtcac cttttaccgt actgccgtcg agaatcaaca caccgccctg ctgtacagta
     7621 atcgttgatt gttgcagctc tgcgccataa ccaataatca ttgaagcgcc gtccagtaat
     7681 gcaacactcc cggtcagttc aaccggtgcg cgatcttcct ctacgacaaa attttcgtcc
     7741 gcgctggtgg gtgacaacag gatcactgtt ccaccttgta ttaccgcatt ttccagcgta
     7801 ccggcatgca cttcagcccg cccccccaca ttcaccgaca ggttttgcgt cttaccggaa
     7861 ctgtagagct gaaggccatc cgtccccaga cgataaatgg atccattctc aacaatagtg
     7921 tcagagtctt caccaccgag attattcatg gtgccaccag caagcaacgt atcactggct
     7981 ttcccggcca gcataaaaac gccgccctga ttaatctcac aattgctggc ttctccactt
     8041 ggatacacca acaattcacc accttcatct accacgaccg ctgaggttgt cccattaacc
     8101 aacaggccgc cctcttgatc gagaatgatt ttttccgcgc gatgtccttc cagtacacgc
     8161 aggttaccgc cattttccag caacaaaccg caggcataac cctgatcaac gctgaattca
     8221 ccttcgggat ggcggccatt aaccgtagcg agcgtagaga gactaattgc gcctccctcg
     8281 gactgaacaa tatctgtggc aatggcaccg tcgctgaccg atagcgtgcc attgttaccg
     8341 tactgtgtgc atttggcgat cccaccggtt gccactttca accagccagc ccccgtgacc
     8401 acggtatttt cggctcttcc gccagcttca acaatcaact cgccttcagt aagttgcgtc
     8461 gcgccaagga cctcgccggt cttaacaatt tgcacattcc cgtcgctcat caagtttttc
     8521 gcatcagatc ccatgattta ttcctttgct gcatctgtgt gcctttattg ctacctaagt
     8581 gtaaaggcta cggaggattt atccacgaca gatttgagat ggtggcaaac aactctgttt
     8641 aaactctgat acacgaatta ttgggttgta tcagatgtaa atgcgatcct gaataaaaat
     8701 cgcccttgca aatcaacaaa atatagccaa tttgcttaaa agctctgcaa acggtcgaaa
     8761 tagcgcagaa aattacgttt tgcctcttgc cacctttcca ctctgccgct aatattcgtc
     8821 cccgttgtca cctacaacgt tgcgttcata gctcagttgg ttagagcacc accttgacat
     8881 ggtgggggtc gttggttcga gtccaattga acgcaccatc ctgcgtccgt agctcagttg
     8941 gttagagcac caccttgaca tggtgggggt cggtggttcg agtccactcg gacgcaccag
     9001 attttcttaa tctggtcttt tcctttttcc ctctgtttct tctctgtatc caatacgtta
     9061 aaagatttac actgtcttcg tatgcgttat cagaaggaga atcgctatgg caactttgtt
     9121 acaacttcat tttgctttta atggcccatt tggcgacgca atggctgagc agcttaaacc
     9181 acttgctgag tcgattaatc aggaacctgg ttttctgtgg aaggtatgga cagaaagtga
     9241 aaagaaccac gaagccggtg ggatctacct tttcactgat gaaaaaagcg cccttgccta
     9301 tctggaaaaa catactgccc gactgaaaaa tctcggcgtt gaggaagttg tcgccaaagt
     9361 tttcgatgtc aatgagccac ttagtcaaat caatcaggca aaactcgcct gacagaattt
     9421 aatcaagggc ggttagcgcc cttttcatcc ctgtctgaaa tttctcaaat tctaaaaatc
     9481 tcaaccaaac ttatctgata acactaaatt cgaaagaatg cgtacaggta agtaacaatg
     9541 aaaaaaattg ctattgtggg tgccgggcct acggggatct acaccttatt ctcgcttcta
     9601 cagcaacaaa ctccactttc tatttctatc ttcgagcagg ctgacgaggc cggtgtcggg
     9661 atgccataca gtgatgagga aaactcaaaa atgatgctgg caaatattgc cagtattgaa
     9721 ataccgccga ttaattgtac gtatctcgaa tggctacaaa agcaagaagc cagccatctc
     9781 cagcgttatg gcgttaaaaa agaaaccttg cacgatcgtc agtttttacc gcgaattctg
     9841 ctgggcgaat atttccgcga tcaattttta cgattagtag accaggcacg acagcaaaaa
     9901 tttgcagtgg ctgtttatga atcatgccag gttaccgatc tgcaaattac aaatgctggc
     9961 gtcatgctcg ctacaaatca ggatttaccc agcgagacgt ttgatttagc ggtgattgcc
    10021 acgggtcacg tctggcctga tgaagaagaa gcaaccagaa cgtattttcc cagcccgtgg
    10081 tcaggcctga tggaagcaaa ggtcgatgcg tgtaacgtgg gtattatggg aacatccttg
    10141 agcggactgg atgcggcaat ggcagtggct attcagcatg gttcgttcat tgaagatgat
    10201 aaacaacacg tcgtttttca ccgcgataac gcaagtgaaa agctaaatat tacgttgatg
    10261 tcgcgcacgg gtattttacc cgaagccgat ttctattgcc ctattcccta cgagccctta
    10321 cacatcgtca ccgatcaggc attaaatgct gagattcaaa aaggtgaaga gggccttttg
    10381 gatcgggtat ttagattgat agtagaggaa atcaagtttg ctgatccaga ctggagccaa
    10441 cgcatagcct tagagagcct gaatgtcgat tcctttgctc aagcctggtt tgccgagcgc
    10501 aaacaacgcg acccatttga ctgggcagaa aaaaatctcc aggaagtcga acgcaataaa
    10561 cgagaaaaac atactgttcc ctggcgttat gtcattctgc gcctgcatga agccgtacag
    10621 gaaattgttc ctcatctgaa tgaacacgac cataaacggt tcagtaaagg ccttgccagg
    10681 gttttcatcg ataattatgc ggcaatccct tcagagtcta ttcgtcgact actggcctta
    10741 cgtgaagcgg ggatctgaaa tccctgatgt tggcgaagat tatacgttac agcaacccga
    10801 agatattcgt ggacgcgtag cgttcggcgc gttgccctgg ttgatgcacg accagccttt
    10861 cgttcaggga cttacggcat gtgcagaaat tggtgaggcg atggctcggg cggtcgtaaa
    10921 gcctgcatcc cgtgctcgtc ggcgtctttc gtttgattaa aggtaaagct atcagcactc
    10981 aacaaactga tgctgttcat taagacgata gcggactccg gttcgaatat tgttttcacc
    11041 ttcaatggcg acggcgaagc gaactctttc gccgtcatga tatgccagtg cggcgcttcc
    11101 ccctagcccg agaataattg agtcgaccac gcccgtgctc actaccgttg aattttcacc
    11161 gctggcaatg atgtgtacat tgtcgccgct gttggcaatt ctggcgtttg cgccaaagct
    11221 ggcgatttgc accaggtcac cattactggc gacatgacac cgttcaccca atgtacaaac
    11281 gcgtactcgc attccggtat tcgcaatccg actactatct ccagcgctgc ttatccgcga
    11341 agaattaccg gttacagcaa cccgtgcgcg ttcaccggag ctgccaatgt gactgttaaa
    11401 gccgacactt ccaatccgca cgctataacc catactggca atttgcgcgg catacccagc
    11461 ggttgcgatt cgggcgttat cctcacaaca gccgatgcga gcatgatcgt cgctgcgggc
    11521 aaactgactg caaaatggta tctctccggg gtctgtggcc agtttttgca tagaggaaac
    11581 ttcctgatgc gcgaagtttt catccgccag ccattttttc cacgcatact caaccagact
    11641 ctccgcccag tccgtatatc cttcctcaat cagtgcatga cgaatatcgg cataactacc
    11701 gccgcgtggg aaatggcgca ggaaccagcg atacatcacc gctccgatac gccattctcg
    11761 caggtctgct cgtgtaatga tcattcgtta gtgccggtga tagccatcga ccatgctttt
    11821 aaaggtttcg tgtggcgtac gccccgtggt gcaaagataa agatgaccga agataaaaaa
    11881 gaggcttata aatgccagag caaaatgcgc ctgcaataac cagtatctta cgccaggaaa
    11941 cacatctccc acggcttgcg gatagagaca caacagcccc gtcaatagta acaacggcag
    12001 caatccatac atgacaccaa cataggcgac ctgctgtaag ggattaaatt tagactgtgt
    12061 tgttgccggg aaaggatgtt cttccccctg cataatgcca aacaaataaa atcgcgtttg
    12121 ttttgccgct cgttccagcc acccctgacg acgaatgcga tagtggtgac cattatcccc
    12181 aacggcattg atcagcacaa agccgagcca gcatgccagt aacaaaaatc cgcaaacttc
    12241 atgcaccgca accagacttt taaccgcagt tgcgcccacc atcgcaaagt gatttatcag
    12301 cccacttgcc agcaacaata caaagagtaa cgcattcgac cagtgccata atctgaccgc
    12361 tttgctgtaa agataaacct tctcaccatg atcggtggct gatttcttca cgccacgagc
    12421 acgaagcaag gcgtgcagcc ccagcaccaa ccacatccca accagcaata ctccggcaat
    12481 gatcaaccac accggccaaa attcgggagt gaactgtggc acatagttcg ctaactggct
    12541 ctgaaactgt tcagcatgtt gcgacgggtt catacatttt cctttttaat caaatgttga
    12601 ccgaaccggc gatacagatg cggttttccg gcaccaggta gctgatattg atagtattta
    12661 ttatcctgca accacgcctg aatttctggg ctatcttcgc gaccaaagat caatgcgtgt
    12721 tccggacagg cgctgacgca aatgggcgga aagccttttg ccagtcggga ctcagcgcag
    12781 aaatcacatt tatctgccac tttagtgaca gggttaaggt agcgaaccag gtacggacaa
    12841 gcgccaatgc aataactgca gccgatgcac tgcgattttt ctacccgcac gatcccctgc
    12901 tcatcacgcc acgatgcccc cgtcgggcat acgtcgatgc acggagcatc ttcgcagtgc
    12961 tgacatgact ggcgaaaaaa gtgatactgc gtttcgttgt cgttatcggt gactggaatg
    13021 tgtgctatcg ataaacggct tccctgagcg gggacgtggt tagttttacg gcatgcgcgg
    13081 gcgcaaatat tacagccatt gcaccgtgac tcgtcatgaa tcatggcata ccgaacttcc
    13141 ttttcttgcc tcgtgttcgc taacagagaa gacgcagaac cagtaaaaaa gattactgtt
    13201 cccatcccca gaacaaattt gcgtcgagtg aaggacatct gattatcccc taatgcagtg
    13261 gctgcaactg tccaccctat ccatgacatc tccctgaaat ttcaggggtt ctgaaactgc
    13321 tctacctcga accattcgca taagccataa gccttgccat aggtcataaa tcgctcgcta
    13381 aacaagtaca acaccgccgt cgatgactcc acctgccgta tatcggcata ttcaggtgca
    13441 acatccatcg cggcgatcgc ggcttcaatc tgcgcttcct ggaagtaata tggcgcttgc
    13501 atcagcatgg cgactttgta aggacgagga tacgtctggc actcaaaacg aacggcgtgt
    13561 gcaatggcgc gacaaatgtc ctgctccaca acttgcaaag acatcgccgc gtagttttca
    13621 ctcatggcct gcgttgagta atagtaatca tcctgacttc ccgtcataca agcgatatcc
    13681 gcgtagtctc cgttctgctt aatgctttcc agcaaaactg acagttcctc ttctgcaacc
    13741 gaatagggtg gcgagagaaa cacggtgcgc gccaccagct gcccagccgc agaatgctgg
    13801 cgaatgaact cagccaggat ttcagcaggt tctggttcgg ttaccgtatc aacggggtga
    13861 cgcatcccct gcaaccattg ccgcttcgcc tcgtcaactt cgctgacttt cgctaacggc
    13921 aattcatcct gatggttcat cttacccatt ccttacgcag gcagtagatt gtgtgctgcc
    13981 agatcggcgg caatatcttc cagccccaga cgctgcaatg tttcgcgggt tggacaacca
    14041 agctgtgggt cccagcccat ttctttgtag aacatcgtga gcgaagcatg catatcgtca
    14101 cgatccattt tgtcagtacc ttcagtaaag accgggatct gcggatcctt gtcgaaaacc
    14161 caggaacaga taagatcgtg ttcattacgc atatctttgg tttgcatcgg ttttaccgtg
    14221 taggcacgat gcaacgtaaa gatacgctct gcagctaaat ctaatttttc ctgagtcatc
    14281 tcttcgccgg tgatcgcttt gaagaatttg gcttcaagcg ccagatcgcc ccggtaatta
    14341 cggcttttca gcggcgaaac ggtcattggc cagacccagt tgcacagagt gacggcgtta
    14401 tgcaaacaga cccgcaacag cgaccacttt gcatatttaa tttttgcgtc attgattggc
    14461 gtgtagtttt tggtttcatc gtaagcatct tcagaaccaa aaagttcttt cgccacttca
    14521 cgttgcagtt tcaatggcaa gccggaacca ataaagttga tatgggtatg cgtcatgcag
    14581 tcacggttga acatacactt aacaatggaa ccgacctgcg ctgacgcttc attggcatgg
    14641 tgaaccggat agccaaacgg cgaccagagt ttatttttcg cgtagcccca gtactcttta
    14701 cccaaattcc agcgttctgc gatggcatat gaaccatcag ccaggtgact cagctcaccc
    14761 acacgatgcg ccagacggta gtaaaaatct ttaatgaagt taacgtcacc cgcttccagt
    14821 tgatcccagc gaatttctgc atactcttca gctggcagaa cacgcttgaa cacacctttg
    14881 ctgtaacaat aagtaaagtc gcgatgcaac tgcccgtagt tacaccatag gccgtagtca
    14941 tcgaacaaat tcagaccaac caggttacca atcacacgac catcgtcttt atcttcaaaa
    15001 tctttcggcc cgttcgggaa gatggtggta tggacaaagt ttgctacaca agtgttacca
    15061 cctgtgctgg gcacgccaaa ctctttcacc cgaggaatat tcatttgggt catacaacgg
    15121 atcgggcaag agtggcagcc gctcattttt actgtgtatt tctctgccgc cggtcctaag
    15181 tcaaaaacgg atttataggt acgaaagccg accgtattct gattgcctgg cggaatttca
    15241 cccgtttcaa tcggaccacc ttcagccgcg ccccaaaaca gccctttacg tgctgtccag
    15301 cgtgacttgg gatctgaata ctctgcccac gattgtggcg tacttggcac gacatggttg
    15361 ttattcgcac caataagttc agtcatcatg taatcattga gacgcttcat ctcctgacga
    15421 tcggcaatgt tgaccccttt cgtcccttca accgcaatcg ctttcaggtt tttcgaaccc
    15481 attattgcgc cagttcccgc accgccgctg tggttacggc tattcaacat gccagagaga
    15541 ggaataaggt tttccccagc ctgaccaata gccgccacac aggtttccgg gctggtcaat
    15601 cgacaaattt cttccgtcgt tgcgcgcgtc ccttttcccc ataagaaatc ggctttttcc
    15661 aggctaactt tgtcatcttt aatcttcagc cataccggtg atttcgcctt cccttcgata
    15721 ataatgacgt cgtatccagc gaatttcatt tgcgctgcaa aaaagccacc catatgggca
    15781 tcgcgactaa atttcctttg gtaaaagtag aaagtgaggt gatatttacg cgagaactac
    15841 agggggcacc agatccaggt aatgggccgg tagcaaagac taatttattc gcttcatcga
    15901 aaggtttcgt gcctagcggt acttcgtcat acataatttt gtagccgaag cccatgccac
    15961 cgacaaaact tttaaactta ctggaatctt cgagggtaat atttcctgtc gtgagattga
    16021 ctcttaatat attacctgtc caaccgttag ccatgatttt ttcctttgca agattcacac
    16081 agtaatatct ttccactcga taatttttaa cgcccctgtt gggcaggcgt ttgcgcattc
    16141 accgcataac acacatttcg aggatttttt actttcggta tttacggtgg ccatcatcca
    16201 cgggcatgcc gtagtacagg cgctacagcc aatacaacgt ttatgatcga cggtaataca
    16261 gccttctttc tgctgccagg taatcgcacc aatcgggcag acgttcatgc attgcggctc
    16321 tttgcattga cggcaggtgt ccgcggtata gttgagatcg ccatacaggc cgccgccaga
    16381 gccaaccccg ttgtcgccaa agaaataatt gcgatggatt ttgatacggg agaagaatgt
    16441 ccctactgag ccatcgttga agttggtaca agagatttca cagcggtggc aacccgtgca
    16501 tcgcgctcgc tgggtcacca gcaccccttt gggtgtattt atcaacccta ctgtgccgct
    16561 atcaatatct tcctgtttgc aacccaaaag tgacagtaac gcaggggcaa ttgttagacc
    16621 agcaaggcct tttcctgata tacgcaagaa ttctaatcgc gttaagccaa tatctaatag
    16681 tggacgatca accgggttca tttatttcgc atcctcttcg caaacacagt gctgacaatt
    16741 cagcagcctg atattaagca ttcaactaat acgctgctga ctctgtcccc aacaccagat
    16801 attttcccta acccgatagt gtatgagaaa agatttaacg ccgataaact aatcctttat
    16861 ggttaggaga aaaaataatg tgattattct gagcccttaa cattgatcgt tatcaattaa
    16921 aattacaaag caagaaatta taagcgaact gatatttatt atcatttgaa ataaatttaa
    16981 cttaagcgac aatcatttgt gacgttcatt gcagaaaata aaaataacat tatcgctata
    17041 aattaaaata cataccgaaa ttcgtataat ttcagcagtt gttatttaga atgattaact
    17101 ggaccgcaac tgaagcaaga atatcaggat gaagcttacc gcctcatcct gaatggtcat
    17161 tttaccagtg cgttaatgac ggtttccatc gcctgctttt ccagctcact gcgctgaatg
    17221 cgtttattgg ctaatgctgt acgtaatacg cctgcgataa caatatgctt tggttcctgc
    17281 cccagatcgc gcatttccag cacaacttta ccgacaactc tgcacatttc tcggtacagt
    17341 tcatcctctt ttgtccgatt tcccattctt tatacctatt tatcatgcca a
//
LOCUS       DAETQL010000081        17265 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.081, whole genome shotgun sequence.
ACCESSION   DAETQL010000081 DAETQL010000000
VERSION     DAETQL010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17265
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.081"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..186
                     /locus_tag="K0W65_003036"
     CDS             <1..186
                     /locus_tag="K0W65_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HBI3096208.1"
                     /translation="YEWQKDVDLHQVLWSRSRLGERPKGQGITGADHFWFGHTPLRHR
                     VDIGNLHYIDTGAVFGG"
     gene            complement(211..402)
                     /locus_tag="K0W65_003037"
     CDS             complement(211..402)
                     /locus_tag="K0W65_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBI3096209.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(507..743)
                     /locus_tag="K0W65_003038"
     CDS             complement(507..743)
                     /locus_tag="K0W65_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HBI3096210.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(861..2300)
                     /gene="rsmF"
                     /locus_tag="K0W65_003039"
                     /pseudo
     CDS             complement(861..2300)
                     /gene="rsmF"
                     /locus_tag="K0W65_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            complement(2380..5000)
                     /gene="yebT"
                     /locus_tag="K0W65_003040"
                     /pseudo
     CDS             complement(2380..5000)
                     /gene="yebT"
                     /locus_tag="K0W65_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4969..6252)
                     /gene="yebS"
                     /locus_tag="K0W65_003041"
     CDS             complement(4969..6252)
                     /gene="yebS"
                     /locus_tag="K0W65_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HBI3096211.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYRRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6382..6879
                     /gene="msrC"
                     /locus_tag="K0W65_003042"
     CDS             6382..6879
                     /gene="msrC"
                     /locus_tag="K0W65_003042"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HBI3096212.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6976..7674
                     /gene="proQ"
                     /locus_tag="K0W65_003043"
     CDS             6976..7674
                     /gene="proQ"
                     /locus_tag="K0W65_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBI3096213.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7694..9742
                     /gene="prc"
                     /locus_tag="K0W65_003044"
     CDS             7694..9742
                     /gene="prc"
                     /locus_tag="K0W65_003044"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBI3096214.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9934..10815
                     /gene="htpX"
                     /locus_tag="K0W65_003045"
     CDS             9934..10815
                     /gene="htpX"
                     /locus_tag="K0W65_003045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBI3096215.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10861..12234)
                     /locus_tag="K0W65_003046"
     CDS             complement(10861..12234)
                     /locus_tag="K0W65_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3096216.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12411..13202
                     /gene="kdgR"
                     /locus_tag="K0W65_003047"
     CDS             12411..13202
                     /gene="kdgR"
                     /locus_tag="K0W65_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBI3096217.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13345..13584)
                     /locus_tag="K0W65_003048"
     CDS             complement(13345..13584)
                     /locus_tag="K0W65_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096218.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13743..13886
                     /gene="mgrB"
                     /locus_tag="K0W65_003049"
     CDS             13743..13886
                     /gene="mgrB"
                     /locus_tag="K0W65_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBI3096219.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13961..14248
                     /locus_tag="K0W65_003050"
     CDS             13961..14248
                     /locus_tag="K0W65_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HBI3096220.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14918..15061
                     /locus_tag="K0W65_003051"
     CDS             14918..15061
                     /locus_tag="K0W65_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBI3096221.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            15074..15283
                     /gene="cspE"
                     /locus_tag="K0W65_003052"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             15074..15283
                     /gene="cspE"
                     /locus_tag="K0W65_003052"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBI3096222.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15449..16258
                     /gene="rlmA"
                     /locus_tag="K0W65_003053"
     CDS             15449..16258
                     /gene="rlmA"
                     /locus_tag="K0W65_003053"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBI3096223.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16255..16821)
                     /gene="mntP"
                     /locus_tag="K0W65_003054"
     CDS             complement(16255..16821)
                     /gene="mntP"
                     /locus_tag="K0W65_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBI3096224.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4563 a         4162 c         4354 g         4186 t
ORIGIN      
        1 tatgaatggc aaaaggacgt tgatttgcat caggtcttgt ggagccgctc gcgattaggt
       61 gaacgcccaa aagggcaggg aattacaggt gctgatcatt tctggtttgg tcatacaccg
      121 ttgcgacatc gcgtggatat tggcaacctg cattatattg ataccggtgc tgtctttggg
      181 ggctaactga ctcttgtgca actgcaataa ttaaaaatca ccatactcct gtgctggttg
      241 ccagaaacca tctataaaat cctcaatcgg aaaacaactg ccatggcgga tccgttgatc
      301 gctcatagaa taaagacact gctgttccgt gttgtagaca tccacaacaa tatcttcaca
      361 accgccatcc aggtagcaaa caaaaagtac cagcgcgaac atttcatccc cgaagtgtgg
      421 tgccgtaccg ttaagtttag gagagatttt acaacggggg aataaccagg acaaataacc
      481 cgccatgccg acgggttctt tttggatcag gcaagacgca taatccaggc atcagattgt
      541 tgatcgtaac gggtacggta aaggacagag tgttcgccat ttagcagggc agggatactg
      601 gtttcagcat cccaggcatc cagttgcata cataaatcgg ggtcagattt acaaggaatg
      661 gttaacgttc gatcacccgg cgattcaccg tgtaattcgc cgtcgtcgat ttcaaaagcg
      721 cctatgcgca cactggtttt catcttcgtg ctctccattt agctgctgat tgtagtacga
      781 ccagtccgat cgcacaattt ttcagcctag ttgaggccgg cgaatgtgcc agtcaaaaag
      841 tgcgcttttt ttgtgcgctg tcatgcgtta ctggtaaaaa gtttcccgtc ccgcaccagt
      901 tcacgcgggt agctgttttt cagtcgcgaa ccaatccgtt tggctaaacc aatcggctga
      961 tgctggaaag taaccaacac gtcatccgcc actggcgcgg cttgcgggta aacatcgcgc
     1021 ccgcgatacc actcctacgc ttcctgcggt gtcagttcaa aagcgtttac attgtcgggg
     1081 gaggcatggg caataaccgc ttcatgctgc cagcgataac ctttgttatg cgtttcggca
     1141 agtttaatcc ctaagcgaga aaatcggact ttaccgatca gggcttcaat gcctaccggg
     1201 aacaaccaca gttctttgtc gcgttgccag agtcgcaggt ttccatccca gtttaagcca
     1261 acacttgcag ccgcctgacg aatttgtccg gcttcgcgat ctttcaccgg gctgaacggg
     1321 aaattgccca ctttgtattt tggggcgggt aaggcgggga tcgcctgagt tttacgcaga
     1381 cgagcaacga agaagccttc gcagtcgtaa atttgtggga aaacatgcaa aaaaccttct
     1441 tcggtcagcg ccttgtttgc accagggaag agatcgccaa gtggtaaaaa ctctacttcg
     1501 tcggggtaag tctctttcag ccaccggcaa acggcttcgt tttcttcccg gtttaaggta
     1561 taggtcgagt aaaccagcgt accgccagga cgtaatgcat gaaaggcgct gtcaatcagt
     1621 tcccgttggg tagctgcgat ttcctgattg ctttcgggtg accagttttt tagcgcatcg
     1681 ggatctttac gcaccacgcc ttcgccggag cagggagcgt ccagcaaaat ggcatcaaac
     1741 atttctggca ctgccgcacc aaacacgcgg ccaccaaaat gtgtgagcgc aacattactg
     1801 atgccacagc ggctgatatt ggcatgtaac acttttaccc aactggcgga aaactcattg
     1861 gcaaggattg ccccttcgtt attcatccgc gcggcaattt gcgtcgtttt tgagcctggc
     1921 gcagcagcga catccatcac ccgctgtggt gcattaccgt cagcaaacaa ggcggcgacg
     1981 ggcaacattg aactggcttc ctgaatataa aacaggccac ttaaatgctc ggtggtacta
     2041 cccaatggca atgcatcttc attgtcgcgt tcaatccaga aaccttcttc acaccacgga
     2101 attggcgtaa gcgtccagcc ataaggagcg gttaattgca ggaaatcagc aacggagatt
     2161 ttcagcgtat taacgcgaat gctgcggcgc aacgggcgct gacaggcggc aagaaaatca
     2221 tcaaatgaga gcgtcgaagg catagcttcg cgcatttgtg tcagaaacgc gtccgggaaa
     2281 taaacggtgt gttgggccac gagcatgtac cactggcaaa aaaacaggcg cgcagtttag
     2341 cataagcgct ccggcgcagg cacgccggag cagtgggcat tatttgggaa gcgcagttcc
     2401 ccattcacgc cactctttcg gttcactttc ctgcaacagg aagtgtttgc cttcttgggc
     2461 tttcggtgca agtggcgtac ccggaggcgt ggcgaaggcg atgccgccac ggataaattg
     2521 gttaaaggtg ccggttttca ctacgccgcc cgtcagacca aagtccagac tgtaacccga
     2581 tgccaaccag aagacggaat tgttacgcac caggtgttga tagcgtttac tgatgcgcat
     2641 cgcaatcatc acgcgatctg acaatgtccc cagcgtcatt ccggtaaccg tacctacttc
     2701 cagaccacgg aacagcacag gcgtaccgat gcctaacgaa ccggcttccg gcgcttcaac
     2761 aataatgctt aaaccatcta ggtaacgcga atcagtaatg gtggcctctt ctaattcaaa
     2821 gtcgcgacga ggattgccac ggcctggttc gacgttgata tacggctgga ggatagtatc
     2881 tagatgctca acgccagctg ccgagatttg cggtgtgacc actgagaagc gcgtaccacc
     2941 gcgagcaaag gtctggacat attccggata gagcaccgcc tttgcctgta cttcattgcg
     3001 tgcggtaatc agatccagcg tctggatttg cccgatatca ataccgagat agcgaattgg
     3061 catcccgacc gccagttttc cggcatcgaa agcgtgaagc gtaatctgcc tgccaaccgc
     3121 acgggccgct gtttcggaag catacagaat tcgcttgtcg cctttacgct gactggcgct
     3181 ggcaccactg aggttatcga agctaatggc cccctttaat gctctggaga gcggggatgc
     3241 ctgtacggtc aggccactac cattcagctg aactttcgcc ccgccttctg cccagaacac
     3301 gctgttgctg gtcaaaaggt tgcgatattc cggtttaatg tgcagatcga tatcgaacgc
     3361 gttagctcgc ggacgcacgg taataacttc accaacttca aatttacggt acagcactac
     3421 cgatcctgcc tgcacatccg gcagcgtctc tgcactcaaa ctcatggttg tggtgggtaa
     3481 atcgctaagg ctgttctcca gcgctttttc cagattggca tacagtggat agctggcttt
     3541 catctcgcct ttatcgcccg gcagaatacg tatcccgccg ttaatccatt ctgaggcgct
     3601 ggcaccgaga aactcaacgc catccagccc caccttcacg tcgacacggc tgttgacgac
     3661 aaatttgcta tcgcctttta ccagttctcg atgctgaggc tcgatggcga cggtaaaggt
     3721 gacgcctttg ctggtgagtt tacgatcgat aacctggcct acctgcacgc cgtgaagaat
     3781 gagcggctga cccgcatcaa taccgtaact ttccggtgct gtcagggtca gtgtcagaac
     3841 gtccggctct tgcagcaggg ctttttcgcc cggcacaaca acgaactctt tgcgtggctc
     3901 gccatcgccg ggcaccaact caaaggtttt tccggtcagc agggcgctga gattagcatc
     3961 gctaagggat aatttcgggt tgcgtaattc gatgcgggta ttttcacgaa gcagggatca
     4021 acggtcattt ccccagtgac tttaccacca ggatttaaat ccagtttagt cagctgtccg
     4081 acttccagcc cctgatacat taacggcgtc gagtcggcgg ttaatcctgc tccactcggc
     4141 agttccagtt ttattattac tccacgctgg ctgtgggcca gatcttcata cagaccaaag
     4201 gtatcttccg cctcggcagg tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta
     4261 accagtgccg ccagactttc cagtttcacc ttcgcgccac tgatactgac gttggcatca
     4321 acgccggaaa cgtttcagaa acggctacct tttttcacca gatcggtaaa acgccgctcg
     4381 atcaggacat caatcaccac gccttgcttg ttgggattga tggcatagtc gtagactttt
     4441 cccaccggga tcttgcggaa atagaccaat gaaccgctgt tcagcgaacc gagagcgggg
     4501 gcttgcaggt ggatcatcag atcgccattg tccagccgat atttcggttg ggtatcgagc
     4561 gcgacaaagt gatcctgctc tttaccttta cccggcatca tgccgatata gttcccaccg
     4621 acgagggcgt ccagcccgga gacacctgcc aacgatgctt ttggcgtcat cagccagaac
     4681 tgagtctctt cgcgcagcgc atctttcata tcggacttga tgctgacctt gacttcaatc
     4741 ttacgaagat cgtcgctgag gctgatatcc tgcactgttc cgacttcaac gccctgataa
     4801 cgaacaggcg tacggcccgg aacaatacca tccgccgaca taaagtcgat ggtgacggta
     4861 ttaccccggt cctgataact gtcccaaatc agccaaccgg caatcattag cgcgatgaaa
     4921 ggcagcagcc agaaaggtga gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa
     4981 gcgggcgttt cctgactcat gtgcatccca aagtaagcgg ctgtccagcc attccacagc
     5041 aagaatagtc aaaattacgg cagcgccaaa ataaaacgca gccggtccca tagtaaaagc
     5101 gaggatctga tcgcgattaa tcagcgacat ggttaaagat atgacaaaca ggtcgagtat
     5161 cgaccagcgt ccaatccagg tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg
     5221 ctggcattta aaatgaatgc tcaacagtaa agtgaacatg acgatcactt tagtaaacgg
     5281 taccagaata ctggcgataa acacgattcc cgcaaccgca atgttgctac ttgccagcga
     5341 cataattccg gaaagaatcg tatcttcctg ccggccacca ttcagataaa tgatagaaat
     5401 aggcaatagg ttggctggta acaacaaaac gattgacgct aacagcgccg cccagcattt
     5461 ttgcaaacta tgacgacgac gcaggcgtag cgggatatgg caacgcgggc agagaccacg
     5521 ctgatcagga tagccggtaa aatggcaccc aagacagaca cgaagtttct cgtccctacg
     5581 cgtagcgggg cgctgcggat aatatcgctc ccacagctcc tcaacattaa gatgtgacaa
     5641 cgtcaccgtc gttaaaatca ccaacgccac aaaagagaac aagccgacac ccgcctggat
     5701 atgggcataa tcctgtacct ttatagaagc aacgccaatg ccgaccaggt agatatccag
     5761 cattacccac tctttaagtc gctcaagcat cagcagtacc ggacgtaaat tcattcccag
     5821 tcggttacca aaccataaat atgctatgga ggacaccaga atgagggggg caccaataac
     5881 gcagaaaaag accattgatc ccgttatcgc atcgccctgt ttggtcattt gccagatgcc
     5941 ttgcatgacg ttggcgtcaa tacgaatgcc taacagccag atatgcaaca gcggttcgcc
     6001 ccaggcaaac ggcataagca acagcatggt gaaggccatt gccgccaggc gcgttagcga
     6061 ccagtcgcgc ccgtcgcgaa tttttgcctg acagcgcgga caataggcac tttgatgaga
     6121 atttatctcg ggcaggctaa acagcatgtc acattgcggg caacgtcggt aatcaccacg
     6181 cggcagttcc tcgccgattg ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt
     6241 gttaagagcc atgacgtgcc aaatgtaata agaattaaca ttaatcttaa cccatgatgg
     6301 gaatgatctt gagcattaac gcatttaatg cttattttaa taggctggca gttccaggta
     6361 agacaaccaa gtgattaagt aatgaacaaa acagaatttt acgcggattt aaatcgcgac
     6421 tttaacgcgc tgatggcggg agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg
     6481 ttgttatacg agcgtctcac tgacgttaac tgggcaggtt tttatttgct tgaggacgat
     6541 acactggtac tcggaccatt tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc
     6601 ggggtgtgcg gcacagcggt tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg
     6661 tttgacgggc atattgcctg tgatgcggcg agtaattctg aaattgttct gccgctggtg
     6721 gtgaaaaatc agattattgg tgttctcgac atcgatagta ctgtcttcgc tcgctttaca
     6781 gacgaggacg agcaaggctt acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg
     6841 acggattaca aaaaattctt tgcgagcgtc gcaggataat caacggataa cgtagcattt
     6901 actggtagcg tcattataat gacgcctgtt catgcctgcg cttgttggct acgtccgttg
     6961 taatcaggaa atttcatgga aaatcaacct aagttgaata gcagtaaaga agtaatcgcg
     7021 tttctggccg aacgttttcc ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa
     7081 attggtattt ttcaggattt ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg
     7141 caattacgat ccgctttacg tctctacact tcgagctggc gttatcttta cggtgttaaa
     7201 cccggcgcaa cgcgtgtcga tcttgacggc aacccatgcg gtgagctgga cgagcaacat
     7261 gtagagcatg ctcgcaagca gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct
     7321 gaacagcaag cgaaaaaacg cgaagctgcc gcagctgctg gtgagaaaga agacgcaccg
     7381 cgccgcgaac gcaagccacg tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa
     7441 cctcgtgcgc aaaagccggt agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa
     7501 cagcacaccc cggtttctga catttcagct ctgactgtcg gacaagccct gaaggtgaaa
     7561 gcgggtcaaa acgcgatgga tgccaccgta ttagaaatca ccaaagacgg cgtccgcgtc
     7621 cagctgaatt cgggtatgtc tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga
     7681 ggccgggcca ggcatgaaca tgttttttag gcttaccgcg ttagctggcc tgcttgcaat
     7741 agcaggccag accttcgctg tagaagatat cacgcgtgct gatcaaattc cggtattaaa
     7801 ggaagagacg cagcatgcga cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca
     7861 ttatcgccag ttcgacctcg atcaggcatt ttcggccaaa atctttgacc gctacctgaa
     7921 tctgctcgat tacagccaca atgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa
     7981 gaaaaccgag ttaggcgatg aactgcgttc aggcaaactc gacgttttct acgatctcta
     8041 caatctggcg caaaagcgcc gttttgaacg ttaccagtac gctttgtcgg tactggaaaa
     8101 gccgatggat ttcaccggca acgacactta taaccttgac cgcagcaaag cgccctggcc
     8161 gaaaaacgag gctgagttga acgcgcagtg ggacagtaaa gtcaaattcg acgagttaag
     8221 cctgaagctg acaggaaaaa cggataaaga aattcgtgaa accctgacac gccgctacaa
     8281 atttgccatt cgtcgtctgg cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac
     8341 ggcgtttgcg cgtgaaatcg atccgcatac caactatctt tccccgcgta ataccgaaca
     8401 gttcaacact gaaatgagtt tgtcactgga aggtattggc gcagtgctgc aaatggatga
     8461 tgactacacc gttatcaatt cgatggtggc aggtggtccg gcagcgaaga gtaaagctat
     8521 cagcgttggt gacaaaattg tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat
     8581 tggctggcgt cttgatgatg tggttgcctt aattaaaggg ccgaagggca gtaaagttcg
     8641 tctggaaatt ttacctgctg gtaaagggac caagacccgt actgtaacgt tgacccgtga
     8701 acgtattcgt ctcgaagacc gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa
     8761 agtcggcgtg ctggatattc cgggcttcta tgtgggtttg acagacgatg tcaaagttca
     8821 actgcagaaa ctggaaaaac agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg
     8881 cggtggggcg ttgaccgaag cggtatcgct ctcaggtctg tttattcctg cgggtcccat
     8941 tgttcaggtc cgcgataaca acggcaaggt tcgtgaagac agcgataccg acgggcaggt
     9001 gttctataaa ggcccgctgg tggtactggt tgaccgtttc agtgcttcgg cttcagaaat
     9061 ctttgccgcg gcaatgcagg attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg
     9121 taaaggcacc gttcagcaat accgttcatt gaaccgtatt tacgatcaga tgttacgtcc
     9181 tgaatggcca gcgctgggtt ctgtgcagta cacgatccag aaattctatc gcgttaacgg
     9241 cggcagtacg caacgtaaag gcgtaacacc agacatcatc atgccgacgg gtaatgaaga
     9301 aacggaaacg ggtgagaaat tcgaagataa cgcgctgccg tgggatagca tcgatgccgc
     9361 gacttatgtg aaatcaggag atttaactgc ctttgaaccg gagctgctga aggaacataa
     9421 tgcgcgtatc gcgaaagatc ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa
     9481 cgctatgaag gacaagcgca atatcgtttc tctgaactac gctgtgcgtg agaaagagaa
     9541 taatgaagat gatgcgacgc gtctggcgcg tttgaacgaa cgctttaaac gagaaggtaa
     9601 accggagttg aagaagctgg atgatctatc gaaagattac caggagccgg atccttatct
     9661 ggatgagacg gtgaatatcg cactcgatct ggcgaagctt gaaaaagcca gacccgcgga
     9721 acaacccgct cccgtcaagt aatatcaatc aggcacaaga aattgtgcct gattttttaa
     9781 cagcggcaag atgccgtaaa tcagatgcta caaaatgtaa agttgtgtct ttctggtgac
     9841 ttacgcacta tccagacttg aaaatagtcg cgtaacccat acgatgtggg tatcgcatat
     9901 tgcgttttgt taaactgagg taaaaagaaa attatgatgc gaatcgcgct cttcctgcta
     9961 acgaacctgg ccgtaatggt cgttttcggg ctggtactga gcctgacagg gatacagtcg
    10021 agcagcgttc aggggctgat gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt
    10081 tcgcttctga tgtccaaatg gatggcatta cgatctgttg gcggggaagt gatcgagcaa
    10141 ccgcgtaacg aaagggaacg ttggctggtc aatactgtag caacccaggc tcgtcaggcg
    10201 gggatcgcta tgccgcaagt ggctatctac catgcgccgg acatcaacgc ttttgcaacc
    10261 ggtgcgcgcc gtgatgcctc tctggttgct gtcagcaccg gtttgctgca gaacatgagc
    10321 ccggatgaag ccgaggcggt aattgctcac gaaatcagcc acatcgccaa tggtgatatg
    10381 gtcaccatga cgctgattca gggcgtggtg aacaccttcg ttatctttat ttcccgtatt
    10441 ctggcgcagc ttgccgcggg ttttatgggc ggaaatcgtg atgaaggtga agagagcaac
    10501 ggcaacccgc tgatctactt tgcggttgca acggttctgg aactggtgtt tggtattctg
    10561 gcgagcatta tcaccatgtg gttctcgcgt catcgtgaat tccatgcaga tgccggttcg
    10621 gcaaaactgg ttggtcgcga gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat
    10681 gaaccgcaag aagcaaccag catgatggct ttctgcatta acggtaagtc gaaatcgctc
    10741 agtgagttgt tcatgactca cccgccgctg gataaacgta ttgaagctct gcgtacgggt
    10801 gaatacctga agtaatatga agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt
    10861 ttatgccctg gctcgtggct gagtgatacg taaaccactg acacaggcag caatcactgc
    10921 cagaatagcc gcagccatca gcgagacgtg cgtaccatta tcaccaaact gatttagcat
    10981 cagcgccacc agcgccgcgc cactactctg acccagtaga cgagccgttc ctaacatgcc
    11041 actggcccca ccgctacgtt cgcgcggagc ggaggtaata atggtgtggt tattgggtga
    11101 ctggaataag ccaaatccag cgccacataa gatcatcggc cagataatat tgatatccgc
    11161 aggtgacgcg ggcagcagaa ccagggaaaa aagccccgta gccatgatga acaaccctaa
    11221 agcacccagc aatcctgcat gtacgcgttc aatcaaatag cctgccagag gagccatcac
    11281 catcgttgct aacggccacg gtgtcagaag taaacctgtt tcgacttcac tacgcccgag
    11341 cacggtttgc aggtaaaagg gcagggaaac cattgccagc atttgtgcgc agaaagagca
    11401 aacagatgtg caaatagaaa gtgaaaacag cgggatacgc agtaaatcca ccggtagcag
    11461 cggtacggga agagaaagct ggcggcgaat aaagaaaata ccaacaacaa ccattaccac
    11521 cagttccgca gcaattaacg tcagcgattg cccctgagcg aaaccactca acgcagtgat
    11581 aagcaggccg aaggttaacg cgttcatcac ggcgctgggc aggtcgaaac ggggtttact
    11641 ggcgcgagaa ccattgggtg gcagaaaacg catcgccaga agcagggcga taatacctaa
    11701 cggtacgttg attaaaaata accatttcca ggatgcgatg gagaggattg ctgcagcaat
    11761 tgtcggcccg gcagcagaag agacggcaac aataaacgag tttatgccca tccctctccc
    11821 cagaaaacgt tgtggataga tcaggcggat aagtgcggta ttaacgctca tcaacgctgc
    11881 accgccgaaa ccttgtatga cacgcgcaag ggtgagcatt tgcagcgaat cagaaagggc
    11941 gcagaacagt gaagacaaca gaaaaacgac cagaccgcat ttataaatac gtcgatagcc
    12001 aaacatatcg cccagaaacg aaaacgagag cagggagatg acaatggcga tttgataggc
    12061 gttcactacc cagatggaac tggctggtgt ggcatgaagg tccgtggcaa ttgttggcag
    12121 ggcgacgttg gcgattgcgc cgtcaaggac agccatcgaa ataccaatca caatggttaa
    12181 tatcgcaccg tatcgctggg gcaatggcag gccgtcggcc tgaacttttg gcataggaat
    12241 tttatatctt tggtgaataa tcaacagggg tatgattcta gcactgatat tcacggtgga
    12301 tgtcgcagaa ttgtaactaa ccggcaaaag atggattgcg accaccctgg tgcgaaatta
    12361 taataaaaaa cagttctgat ttttataaaa cactcgcaat gaggtgataa atggctaacg
    12421 cagatctgga taaacagcct gattctgtat cttccgtgct aaaagttttt ggcattttgc
    12481 aggcgctggg tgaagagcgc gaaataggga taaccgagct gtcgcagcgc gtcatgatgt
    12541 caaaaagcac cgtttatcgc tttttacaga ccatgaaaac cttaggttat gtggcgcagg
    12601 aaggggagtc ggagaaatat tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt
    12661 tacaaaacgt cgatttaatt cgtagcgcag atatccagat gcgtgagatc tcccgcctga
    12721 ccaaagaaac tatccacctc ggcgcactgg acgaagacag tattgtttac attcacaaaa
    12781 ttgactctat gtacaatttg cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca
    12841 gcaccgcgat tggtaaggta ctgctggcat ggcgcgatcg cgatgaagtg aagcaaattc
    12901 ttgagggcgt ggagtataaa cgcagtaccg agcggaccat caccagtaca gaagcgttat
    12961 tacccgttct ggaccaggtg cgcgagcagg ggtatggaga agataacgaa gagcaggaag
    13021 aagggctgca atgcattgcg gtaccggtat ttgatcgctt tggcgtggtc attgccggtt
    13081 tgagcatctc cttcccgacg ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa
    13141 tgttgcatac cgcagcgcgc aaaatttctg cccaaatggg ttatcacgac tatccgttct
    13201 gatgagagta agaacctgtc ggaatatcaa acagacaggt tctttattta gcatgagaaa
    13261 aataaagttg aaggtgcgtt atattaaacg cgcttgctat aagaatattt tactcatgag
    13321 tgagaatctg gttatttatt acccttaacc attatcgacc acgatattgc ttttacgtaa
    13381 cagtgggcaa tctgttatct ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct
    13441 ggtgcctctg gcagtcagat aggtacattg caaacctaat cctgcggcat tctctttgct
    13501 tccaatcaaa acgccatatc cgctgagtaa taatcctatc cataccagtg ctatcagcat
    13561 aactgtgcga atgatgaatc gcattacaat ctcttctctt tttatgttcg cttaatcgta
    13621 gcggcaatat gcgctgaagc aagctactca ttccgaaaaa gcacgaatat cgacatagtt
    13681 aggcgctgtt taactaacgc atgctagttt aatgacataa ggtaggtgaa gcggagattg
    13741 gagtgaaaaa gtttcgatgg gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt
    13801 gggcgcaggt attcaacatg atgtgcgatc aggatgtaca atttttcagc ggaatttgtg
    13861 ccattaacca gtttatcccg tggtgatatc attttttcag cactagattt tcttcctctc
    13921 cagcagtggt agaatagcgc ctctactcag aggtggtgaa atgaacgaag ttgtaaattc
    13981 aggcgtgatg aacattgcgt ctttggttgt atcggtggtg gttcttctta tcgggctcat
    14041 cttgtggttt tttatcaatc gtgccagttc acggactaac gaacagattg aactgcttga
    14101 ggcgttgctg gatcagcaaa aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa
    14161 cgaaccagag aaagcagata aaaagaccat tgagagtcaa aaatcggttg atgacgaaga
    14221 tattattcgc ctggtcgccg aacgataatc cttcctcaat cttgagtaac cccgtcgttg
    14281 aagggttact caattcatcc tgattatacg cactgtcatg gtgcaactgc ttcaaattaa
    14341 aactctggtc tgacttcaaa taattatcat attatttatt tgcgtaacgg gatatcagaa
    14401 aaagatattt tcctcattaa tttattagga tgtttacatc agatttgtga ttaagcgtgg
    14461 tattatttat tagcgaaacg tttctctctt gatttttttg ctcactcatc aatttttctt
    14521 attttaaatt tacaatccct ttggggattg acttctcttt agggtaattg atagccgtta
    14581 actgactgtt ttatgagaaa aagtgatata actttttatt cattgcatag cagaaaatgt
    14641 gatattgcac gcactatgta ataacttctc cctctggcct ggaacaactg aacttattga
    14701 actatgttag aaactacgcc agtttaagta tctgcctgaa ctggcaaggt taagcacaat
    14761 gatatatcgg cgcgtattcc gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga
    14821 tttgtgcgtc tgatcgagat atgtttaaaa atggcttgcc ataattaacg ttgtatgtga
    14881 taaaagattt cgggttaaac gaggtacagt tctgtttatg tgtggcattt tcagtaaaga
    14941 agtcctgagt aaacacgttg acgttgaata ccgcttctct gccgagcctt atattggtgc
    15001 ctcatgcagt aatgtgtcag ttttatctat gttatgcctg cgggcgaaga aaacaatcta
    15061 aggaattttt caaatggcaa agattaaagg tcaggttaag tggttcaacg agtctaaagg
    15121 ttttggcttc attactccgg ctgatggcag caaagatgtg ttcgtacact tctccgctat
    15181 ccagggtaat ggcttcaaaa ctctggctga aggtcagaac gttgagttcg aaattcagga
    15241 cggccagaaa ggtccggcag ctgttaacgt aacagctatc tgatcgaatc cactgatctg
    15301 aagtgtgaat acgcttcaat ctcgctataa agcctcgtcg aatgcgaggc tttttactat
    15361 gctttatctt cgctcctggc gttcggatat ttgcccgccg cgtgattcgc gttacacttg
    15421 cggcctttag tatcctgccg gagttgtcat gtcgttttcc tgtccacttt gccatcagcc
    15481 tctttcgcgt gaaaaaaaca gctatatctg tccccaacga catcagtttg atatggcgaa
    15541 agaagggtat gtcaatctgc tgcccgttca gcataaacgg tctcgtgatc cgggcgacag
    15601 cgcggaaatg atgcaagcac gccgcgcatt cttagatgcc ggacattatc agccgctgcg
    15661 tgatgcaatt gtcgcccaac tgcgggaacg gcttgatgaa aaggccacgg cggtgctgga
    15721 tattggctgt ggtgaagggt attacacaca cgcatttgcc gatgcgttgc ccgaaatcac
    15781 cacgtttggc ctggatgtct cgaaggtagc gataaaagcg gcggcgaaac gctatccgca
    15841 ggtcactctt tgtgtcgctt ccagccaccg tttgccgttt tccgatacca gtatggacgc
    15901 cataatacgt atttacgcgc cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg
    15961 cggctgggtc attactgcca cgccgggacc gcgacatttg atggagctga aggggctgat
    16021 ttacaatgaa gtacatcttc atgcacctca tgcagaacaa ctggaaggtt ttacattaca
    16081 gcagagtgcg gagttgtgtt atccgatgcg tcttcgcggt gatgaagccg tcgcattatt
    16141 gcagatgacg ccgtttgcct ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa
    16201 agaagtgttc gactgccaga cggactttaa tattcacctc tggcagcgtt cttattaacc
    16261 gtggaagtgc gtccagagga tctggacgcc gatgccgatc agcaccagcc cgccgagaat
    16321 ttccgctttt ttcccaataa ttgagccgat aaagcgacca accatcatcc ctaatgtcga
    16381 cataatcaag gttgcacaac caatggccaa tgcggtcgcg ataatgttga cctgaaggaa
    16441 agcaagacca acacccacag ccatggcatc caggctggtg gcaatcgcgg tggttaccag
    16501 tagccagaaa ccgtgccgac ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc
    16561 ctcaataatc attcgcccgc cgaggaatat cagcagcaca aacgcaatcc agtgattcca
    16621 ttcaaggaca aaccggctgg ctaacatgcc cattccccag ccgatcagcg gcgtcagggt
    16681 ttcgacggca ccaaaaataa ggccggttcg caatgcttca gaaaatttcg gtttatggag
    16741 gggggcacct ttaccgattg atgcagcaaa tgcatccatc gacataccaa acgcaagaag
    16801 aacagtagca gtgatattca tgacaacgtc ctgaccgggg tatccataaa acacatcatc
    16861 gcccccagca aacatgcata catgaacaac aagccgcgat gggttttcgc gttcatgcag
    16921 gcatagcgat tgatgtgcat atggtctcgc ctgactgaaa gtcttcagtc cgtacgtgcc
    16981 acgttttgca acgagtatgt tgacacgtac atttccggaa tctggaaatc ggctactccc
    17041 caaatgaggg cgcaacctta acatattttg agaatataaa acaactgttg gaatatatta
    17101 tttcattgct ttattgataa cgattttcat ttatgtttat atatcaatga aacgttaaaa
    17161 taaataacaa ataagggaag agataatata gcctaagcta tgtctgatta ttaaaaatac
    17221 actaaacata gctttggcta aattcataag ttattgagtt gatac
//
LOCUS       DAETQL010000082        17093 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.082, whole genome shotgun sequence.
ACCESSION   DAETQL010000082 DAETQL010000000
VERSION     DAETQL010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.082"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..510
                     /locus_tag="K0W65_003055"
     CDS             <1..510
                     /locus_tag="K0W65_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBI3096225.1"
                     /translation="PFREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDG
                     FTQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATI
                     RHNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADR
                     QYSRAWTVK"
     gene            complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="K0W65_003056"
     CDS             complement(511..1671)
                     /gene="ybdL"
                     /locus_tag="K0W65_003056"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HBI3096226.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1780..2868
                     /gene="hcxA"
                     /locus_tag="K0W65_003057"
     CDS             1780..2868
                     /gene="hcxA"
                     /locus_tag="K0W65_003057"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HBI3096227.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2878..3075)
                     /locus_tag="K0W65_003058"
     CDS             complement(2878..3075)
                     /locus_tag="K0W65_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBI3096228.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="K0W65_003059"
     CDS             complement(3257..5362)
                     /gene="cstA"
                     /locus_tag="K0W65_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HBI3096229.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5543..5956)
                     /gene="entH"
                     /locus_tag="K0W65_003060"
     CDS             complement(5543..5956)
                     /gene="entH"
                     /locus_tag="K0W65_003060"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HBI3096230.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5959..6705)
                     /gene="entA"
                     /locus_tag="K0W65_003061"
     CDS             complement(5959..6705)
                     /gene="entA"
                     /locus_tag="K0W65_003061"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HBI3096231.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6705..7562)
                     /gene="entB"
                     /locus_tag="K0W65_003062"
     CDS             complement(6705..7562)
                     /gene="entB"
                     /locus_tag="K0W65_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HBI3096232.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7576..9186)
                     /gene="entE"
                     /locus_tag="K0W65_003063"
     CDS             complement(7576..9186)
                     /gene="entE"
                     /locus_tag="K0W65_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HBI3096233.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9196..10371)
                     /gene="entC"
                     /locus_tag="K0W65_003064"
                     /pseudo
     CDS             complement(9196..10371)
                     /gene="entC"
                     /locus_tag="K0W65_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10663..11619
                     /gene="fepB"
                     /locus_tag="K0W65_003065"
     CDS             10663..11619
                     /gene="fepB"
                     /locus_tag="K0W65_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HBI3096234.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11623..12855)
                     /gene="entS"
                     /locus_tag="K0W65_003066"
     CDS             complement(11623..12855)
                     /gene="entS"
                     /locus_tag="K0W65_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HBI3096235.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12966..13970
                     /gene="fepD"
                     /locus_tag="K0W65_003067"
     CDS             12966..13970
                     /gene="fepD"
                     /locus_tag="K0W65_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HBI3096236.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13967..14959
                     /gene="fepG"
                     /locus_tag="K0W65_003068"
     CDS             13967..14959
                     /gene="fepG"
                     /locus_tag="K0W65_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HBI3096237.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14956..15771
                     /gene="fepC"
                     /locus_tag="K0W65_003069"
     CDS             14956..15771
                     /gene="fepC"
                     /locus_tag="K0W65_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HBI3096238.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15768..16901)
                     /gene="fepE"
                     /locus_tag="K0W65_003070"
                     /pseudo
     CDS             complement(15768..16901)
                     /gene="fepE"
                     /locus_tag="K0W65_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3993 a         4865 c         4444 g         3791 t
ORIGIN      
        1 ccctttcgtg aaaagccagt ggattgcgtt ctgtgggtta aaaacagcca acttatgcct
       61 aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgca aaaatccatt
      121 gaaatagatg gttttaccca acccattgtg gtcacacata cggataaaaa tgccatggaa
      181 atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt aaaattacgc
      241 ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg catcgccgcg
      301 actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc ggaaatcgtc
      361 cgtgaactta gccagttggg atgggatgat aataaaattg gcaaagagct gggaatggac
      421 agcgacgaag tgctgcgact gaagcaaatt aacggcctgc aagagttgtt tgccgaccgt
      481 caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt cagctgctgc
      541 cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg ggaagggatc
      601 ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca gccactggca
      661 aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa agtatgtacc
      721 ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa tatcgcgctt
      781 ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta gcatatcagc
      841 aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta ccttgcgaat
      901 ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg tcatatgata
      961 ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat gcgccagcac
     1021 actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc taatgacaaa
     1081 aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct gccagacagt
     1141 tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc ttaataatgc
     1201 ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac gtttcactat
     1261 tcccccagaa agcgcgatgg cgggggcgta actgtcatag ctgggatcaa aacaaatcac
     1321 ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg tcgcccctgc
     1381 cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt ccgttttctg
     1441 agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt ttgccccctg
     1501 ggcaacgtgg tacgccagcc gctcctgtaa atagcgcgga ccatcaaaat caggaaagcc
     1561 ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg tgaaaatagt
     1621 ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca tttttattgt
     1681 acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ccagtatttg gcaatcaaga
     1741 cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa tcctatccgc
     1801 gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatca cctgcacaat
     1861 tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg cgccattgct
     1921 gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca tattttgttt
     1981 cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg tgacgatcgc
     2041 agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc cctcgcccgc
     2101 cgtctcggtc tgccatttgt tgccgctccg acgatcgccg ccacctgcgc agcctggaca
     2161 ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat tttcgacgac
     2221 gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca acaatatctg
     2281 ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct ggctccgcaa
     2341 ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc cattcgcgac
     2401 gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca gttaacgcaa
     2461 tcattttgcg atgtggtgga tgctattatt gctggtggtg ggatggtcgg tggtctgggc
     2521 gatcgtttta cgcgtgtggc ggcggctcat gccgtgcata acggtctgac tgtcctgccg
     2581 caaaccgaga agtttcttca cggcaccaaa gtcgcttacg gaattctggt gcaaagcgcc
     2641 ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg ttttcatctg
     2701 ccgactacgc tggcggagct ggaggtggat atcaataatc aggcggagat cgacaaagtg
     2761 attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac gctgacacca
     2821 gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg tgcaaattta
     2881 gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac ggaaaaactc
     2941 ctcataggtc atcggcgttt gatcgggatg attaacccgc atatgttcga catagttgtc
     3001 gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc cggcttttgc
     3061 cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc cggatgcggc
     3121 gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc cctcgcccca
     3181 tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt cgccaacatc
     3241 ccccctcact ctgactttag tgcgcgcctt tcgcctgcgc tacgatctcc tcgacatttt
     3301 ccggcatcgg ctcatacggc gtttctttcg ccgttggctt cggatctttc aatgccgcaa
     3361 gtgccgtctt aatcgagaac agtgccagga ccaccacgac caccataaag aagatggtta
     3421 acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca gtatactgcg
     3481 acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatgacc aggaagccga
     3541 ctttcgcatc cgggctaaac gctttcagcc agtcagcggt cagggtacaa atcagcagcc
     3601 aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg aacaacacca
     3661 cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca aacagcggcc
     3721 acagagtgtt aataccgccc agcggatcga ccacgctctg atggaggaag tagccccacg
     3781 ccagtacgca cagcgctgtt gccagcaggt tagcaggcag tgaatcggtc cgcttcaggc
     3841 caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc gtacccgcat
     3901 ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac cagaacgcca
     3961 catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc gccaacgtcg
     4021 gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca atctggttga
     4081 gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca gaagcgacta
     4141 catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca aaatacacgc
     4201 ccggatcgat gatacaggca gaaactagcg ccataatcgc cacgaaggat tccatcaaca
     4261 tcccaccgta gccgataaag cacgcctgcc cttcgttcgc cagcatcttc ggcgtggtgc
     4321 cggaagagat cagcgcatgg aagccagaca ccgcaccaca ggcgatggtg ataaacagga
     4381 acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg gtcagcgcag
     4441 gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg atagtcccga
     4501 ttttcaggaa ggtcgagaga tagtcacgcg gggccagcag caaccacacc ggcagcaccg
     4561 ccgccacaaa accgtagccc accagcatcc aggtcagctg cacgccggta aagtcaaagt
     4621 acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg aaaatcagga
     4681 acaccaggcc aatcactgac acttcgccaa tgcggcccgg acgcagatag cgcaggtaga
     4741 ttcccataaa aatcgccagc ggaatggtga acgcaacggt atacgttccc cacgggctat
     4801 gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc atgatcataa
     4861 agcaggctac cagcgcaatc accccggcag ttggccccat ctcttctttg accagctcac
     4921 ccagcgagcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc tgcaccgcac
     4981 cggcgagaac caccccggcg agcagccaga tcatccccgg caggtagccc atttgcgctg
     5041 ccagcaccgg ccccaccagc ggccctgctc cggcaatggc cgcaaaatgg tgaccgaaca
     5101 gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc gctggcgtca
     5161 tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa aaacggtaag
     5221 cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc tgttccccac
     5281 gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact gtccagacga
     5341 ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga gatgttacat
     5401 tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa atcggagttg
     5461 aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag ttttcctgca
     5521 atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga caagtgcagc
     5581 aacgccgccc ctgttcatcg aaaacgacga tttcccaact ctgattttgc cgcccaagat
     5641 gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga tgggtggcgt
     5701 taagttccgt gcccaccaca cactgcccgt cacgggtcat cataaatccg gccatcgatc
     5761 ccagcgtttc agccagcgcc gccgacgcgc cgccatgcag caggccaaac ggctgatgag
     5821 tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagaagg gtatacacaa
     5881 ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg agcgttaaat
     5941 ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca caatatcctg
     6001 tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg cgatctcttg
     6061 tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa agccgcgaat
     6121 acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg tgtcggtgga
     6181 gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc cgacgctcaa
     6241 cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcataccaa tacgcgccgt
     6301 gtgtgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc gaaactggtt
     6361 catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag tctgctgcca
     6421 gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg cattgatcag
     6481 cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcca cctgcccggc
     6541 gtcggcaaca tccattactt cggtcgcaaa gggatattgc tcctgagtga acgcttgatc
     6601 aaaacctgta actttcgctc ccgcctcaac aaacgtcagc gccgtggcgt agccgatacc
     6661 tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt cacctcgcgg
     6721 gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac aaagtcgata
     6781 tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga atccagaccg
     6841 tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa cggcaggatc
     6901 acctcacgca gcgccgcttt gctggcgggg ataggtgctg gcagtaattc ttcggtcatc
     6961 accacccggc cagaacgtcc ggccacatat ttcagcgaca tcaaatgctc gtcacggctg
     7021 aaatcagcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa tgcgtcggtt
     7081 gcggtggtca tacagccaat gtgggcatat actcctgtaa taatcagctg gttacgtcca
     7141 ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg ccacttcacc
     7201 agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg ttccggcgag
     7261 cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc gctctgctct
     7321 ttcggctggg cggtgtaata aaccgggata ttgtgctgtt tgcagtagtc gcgcagcgca
     7381 gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa gctgacaaaa
     7441 tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc ccagtcaact
     7501 ttattctgcg gaatatcgtg agactccggc agtgcgtaag cctgtaattt tggaatagcc
     7561 atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta attgtttttt
     7621 atcgactttc ccgaccgccg tcagcggaag tgaatccaca cactccacgc gatccggtaa
     7681 tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg cgcgcagcgg
     7741 ctcttttacc accagataag cacagctttt ttcgcccatc agctcatttt ccatgctcac
     7801 cagtgcggca tagatcactg ccggatggct cagcagcagg ttttcgatat cttcggcagc
     7861 gatcttctcg ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg tgatgtaacc
     7921 ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat caaaggcact
     7981 ggcattgtgc tgcggacttt tgtaatagcc acggaaggtg tacggcccgc gcgtcatcag
     8041 gcgtccaact tccccttgcg gcagtggatt tccttcggca tcggcaaccc acacttcatc
     8101 gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat catcaagacg
     8161 ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaactggc agccaatctc
     8221 agcgggaata cgcgctgcaa gcgtggcaga aagacgtgcg ccgccgacct gtaacagttt
     8281 cagcgaggca agctgcgccc ggctttcgcc ttcagccagc gcctgcaacc acaggctgac
     8341 tgccggcggc accagcgcgg taacgttaac ctgatgtttt gcaatcaacg ggaagcaaag
     8401 cgtggcgctg ggatcggccg ccagaacaac cgttcctccg gcaagaaaga cgcccagcga
     8461 tcctggcgaa ctcatagtgt agttatgagc cgccgggatc gagcacaggt agcgcgtctg
     8521 ttgtgtgaac tgacaaatct cggcgctacg acgcacgctg tagtaatagt cgttatgagt
     8581 gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat aggccacttc
     8641 atcagcaggt gatggcgtgg cagtaaaacc ctctgccgga tggttaatcg catcctgcaa
     8701 gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaagaat gctctgcaac
     8761 aaatgtgttg aggaaatcat ccccgctaaa tagcgcatgc tggcgatcgg caatcagtaa
     8821 cgccggttca atctggctgg cgtaggcgtt cagttcacta cgctgatggc tgaacagcgc
     8881 cagcaccggc gcgacgccca gtttcagcag cgcgaaaaag gtaatgtaaa gttcagcgac
     8941 gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc gcaaactaca
     9001 ggcgaggtta tccgccgcct gattcagctc ccgataactc aactgccgct cgccgtcgat
     9061 aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagtg gcaaatcctg
     9121 ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg tgaatggaat
     9181 gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa gtttgacgcc
     9241 tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc cggcaaacag
     9301 acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt cgccgttacc
     9361 ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaacg gctccagttc
     9421 agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg tcggatgcag
     9481 cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa agggagttgc
     9541 gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt gtaactcact
     9601 actgcgttcg cgcagtacct ctttcatcgc ctgagtcccc agttcatgtt catggcgatc
     9661 tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat ccggctgacg
     9721 acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac gtagcagcag
     9781 ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga agttgtaact
     9841 aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg cggcgtcagt
     9901 ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg cggtaagtgc
     9961 ggcggcgcgg gcacccatct gttcaaacgt ggtttgttca ggaattgcct ggcgttccac
    10021 cacgttcagc gactggctgc gggtaaaaca gcgggctgag gtctgttttt cctgacggga
    10081 gaacgactgc taggattcgg gaatatacag cgacgaaggc tgacgtggat cgaagggaat
    10141 cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa acagcgcggc
    10201 gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt catcgaaacg
    10261 ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa agcgattggg
    10321 cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca tatcatcctc
    10381 cacaaaatga taaaggcttt aataatgatt atcacttata ttttcggccg ctaacctaaa
    10441 agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg ggttgcatct
    10501 ggttgtggtt acaatgaaaa tgagaagcat tattgatgaa ttcgcataag cgcaatgtga
    10561 tgtcctgcgc cgttttgccc cctctcccca aagggacgga ttgtgcgctt tgtcgaattt
    10621 gtcattacgc ccttaacctt attaataaca ggaagctgac ttgtgagact cgccccgctc
    10681 taccgcaacg cccttctatt aacaggactt ttgctttcag gaatagccgt agttcaggcc
    10741 gccgactggc cgcgtcagat tactgacagc cggggcactc ataccctgga aagccagccg
    10801 cagcgtattg tttccaccag cgtcaccctg accggatcac tgctggcgat tgatgctccg
    10861 gtgatcgcca gcggcgcgac cacgccgaat aaccgcgtcg cagatgacca gggcttttta
    10921 cgccagtgga gcaaggtggc gaaagaacgc aaactgcaac ggctctatat cggcgaaccg
    10981 agcgccgaag ccgttgccgc gcaaatgccg gatctgattt taattagcgc aaccggcggg
    11041 gattcggcgc tggcgctgta tgatcagctt tccaccatcg ccccgacatt aatcatcaat
    11101 tacgacgaca aaagctggca gtcgctgtta acgcagcttg gcgaaattac cgggcatgag
    11161 aaacaagcgg cagagcggat tgcacagttt gataagcaac tggcagcggc gaaagagcaa
    11221 atcaaattac cgccgcagcc ggtcactgcc attgtctata ccgccgctgc acacagtgcc
    11281 aatctctgga cgccggaatc agcacaaggg cagatgctgg aacaactcgg ctttacgctg
    11341 gcgaaattgc ccgcagggtt aaacgccagc caaagccagg gcaaacgcca tgacatcatt
    11401 cagcttggtg gggaaaatct ggctgcgggg ttaaacggtg aatcgctatt cctgttcgct
    11461 ggtgatcaga aagatgccga tgcgatttat gccaatccac tgctcgcgca cctgcctgcg
    11521 gtacaaaaca agcaggttta tgcgctggga accgagacgt tccgcctgga ttactacagc
    11581 gccatgcaag tgctggaacg gcttaaggcg ctgttttaag cattaactgt cggacgctgt
    11641 cacctgcggc ggcgtctggc gaaaacgtcg caactccacc agcaccagca ataacaacac
    11701 gccgataatc aacaaaccaa aaccgctcgc gcttgcggaa gcaaccggtg tcatcatcgc
    11761 gcccaaacca cccagcagcg ccgcgcctat ggcatcgccc gtcacgttct gcgccgtcca
    11821 caaaccgtta atccgcccta acatcgcttc cggggtttgc gtttgcagca ttgtatattg
    11881 cagcaacgaa ctaaccgcgc tcagccagcc gaacagcgcc agacaaacca cgcctaaaat
    11941 ccacatcggc atcaggccaa acagaccaat ggcgaggaac gatcccagcg tggagagcag
    12001 catcaataac cctggtcgcg cactatgtgc cagcttcccg ctggttaacg caccaatagc
    12061 cgcgccgagc gggatcgccg cgtagagaaa accaatctgt gccgctgaca tctgccagtt
    12121 gtcagccagc gccggataca gtaccagcac cgcgctcgcc atcgtcaata aaccacccag
    12181 cagcgggctg gcaagcagaa aacgaaatcc tgccagtaat gatttcaacg gatgctcaag
    12241 cggctgcggt ggcggtggca actccggaag gcttaataac ggtaacaagg taataaacgt
    12301 gcccgccgcc gccagcccgt agttccaggc tacgccgccg gtcgccagca ataaaccgcc
    12361 aatcatgggc gaaatcaccg accccaggcg cacggtcaac atggtgatcg ccccggcctg
    12421 cattaagttt tcacgcccta ccagtgcaga tgttgccgcc agtagcgccg taacaccaag
    12481 tgatgcgaaa aaaccatccc ataaaccaag taaatagatt gccagcaatg acggctccgg
    12541 cagcagtgca ttaaggcaca gtccaatgaa gccaatgcca caggtgccgc gcgccagcaa
    12601 aatcactttt ttgcgttcat agcgatccgc cagcacaccg ccaaccatca ggccaacaaa
    12661 catcgcgccg ccggtcagcg tcaccgaaag ccccacctgc caggtagaat gggtcatcat
    12721 ctggatctgc accggcaccg cgacgccgag caaacccaga gacacaattg agataaaacg
    12781 agcgaggaat actgcgcgaa acgccgggtg cgttttcaac aggctgaggt taagcagcca
    12841 ggattgttta ttcattacaa tgccttgcca tcaaatattt taaataccta ttcttaggct
    12901 gcacatgtta acatatccaa ataagatcga taacgataat taatttcatt atcatggaag
    12961 ttcgtatatc tggttctgtt gccgtgacac gcgccattgc cgtgcccgga ttgctgttat
    13021 tactgattat cgcgacggca ttaagcctgc tcattggggc aaaatcactc cccgcttccg
    13081 tagtgctgga ggccttctcc ggcacctgcc agagcgccga ctgcaccatc gtgctcgacg
    13141 cgcggctgcc gcgtaccctt gccggtttac tggcaggcgg cgcgcttggc cttgccgggg
    13201 cgttaatgca aaccctcacc cgaaacccac ttgccgaccc cggcttgctt ggcgtgaacg
    13261 ccggagccag ctttgccatt gtgctgggtg cggcgctgtt tggttactca tccgcacagg
    13321 aacaactggc gatggccttc gccggggcgc tggtggcctc attgattgtt gcctttaccg
    13381 gcagtcaggg tggagggcag ttaagtccgg tgcgtttaac cctggcgggc gtggcgctgg
    13441 cggcggtgct ggaaggactg accagcggca tcgccctgct taatcctgac gtctacgatc
    13501 aactgcgttt ctggcaagcc ggttcgctgg atattcgcaa tctacatacc ttaaaagtgg
    13561 tgctgatccc ggttctgatc gccggagcaa ctgcgctatt actgagtcgc gcgctgaaca
    13621 gtttgagcct cggcagtgac accgcgacgg cgctgggcag tcgcgtggcg cgcacacagt
    13681 tgattggtct gctggcgatt accgtgcttt gtggtagtgc gacggcggta gttggcccga
    13741 ttgcctttat cggcctgatg atgccgcaca tggcgcgctg gctggtaggt gccgatcatc
    13801 gctggtcgct gcccgtcacg ctacttgcta cccctgccct gctgctgttt gccgatatca
    13861 tcgggcgggt gattgtgccc ggcgaactgc gcgtttctgt ggtcagtgcg tttattggtg
    13921 caccggtgct gatcttcctc gtgcgacgta aaacgcgagg tggtgcatga tttacgtctc
    13981 tcgccgatta ctcatcacct gtttgctgct gatttccgcc tgtgtggttg caggtatctg
    14041 gggattacgc agcggtgccg tcacgctgga aatctcgcag gtattcgccg cgttgatggg
    14101 cgatgcgccg cgcagtatga cgatggtggt caccgaatgg cgtttaccac gcgtgctgat
    14161 ggcgctgttg attggcgcgg cgctgggcgt cagcggcgcg atttttcagt cgctgatgcg
    14221 taacccgctc ggcagccctg acgtaatggg ctttaacacc ggggcgtgga gcggcgtgct
    14281 ggtggcgatg gtgctgtttg gtcaggatct gacggctatc gcgctggcag caatggtggg
    14341 cggcattatc acttcgctgt tggtctggct gctcgcctgg cgtaacggta tcgacacctt
    14401 tcggttgatt attatcggta tcggcgttcg cgccatgctg gtggccttta atacctggct
    14461 gttgctgaaa gcgtctttag aaacggcgct aacagcaggt ttgtggaatg ccggatcgct
    14521 caacggcctg acgtgggcaa aaacctcgcc ttccgcaccc atcattatat tgatgctcat
    14581 tgccgccacc ttgctggtgc ggcgaatgcg cttgctggag atgggcgatg ataccgcctg
    14641 cgcgctgggc gtcagcgtcg agcgttcgcg tctgttaatg atgctggttg cagtggtgct
    14701 taccgctgcg gcaacggcgc ttgccgggcc gatttccttt attgctttag tcgcgccgca
    14761 cattgcccga cgcattagcg gcaccgctcg ctgggggctg acccaggcgg cgctgtgcgg
    14821 agcgctgtta ctgctggcgg ccgttctctg cgcccaacaa ctgtttatgc cgtatcaact
    14881 tccggttggc gtcgttaccg tcagcctcgg cggtatttac cttatcgtct tgttaattca
    14941 ggagtctcgc aaaaaatgac cgaatcagta gcccgtttgc gcggcgaaca gttaaccctg
    15001 ggatatggca aatataccgt tgcggaaaat ctgactgtag aaattcctga tggtcacttc
    15061 acggcaatta tcggaccaaa tggctgtggt aaatccacgt tactgcgtac cttaagccgc
    15121 ctgatgacgc ctgctcacgg gcatgtctgg ctggatggcg agcacattca acattacgcc
    15181 agtaaagaag ttgcacgccg gattggtctg ttggcgcaaa atgctaccac gccgggcgat
    15241 atcaccgtgc aggagctggt ggcgcgtgga cgttatccgc atcaaccgct gtttacccgc
    15301 tggcgcaaag aggatgaaga agcggtaacg aaagcgatgc aggccacggg aataactcat
    15361 ctggcagatc aaagcgtgga taccctttct ggcggacaac gccagcgagc gtggatcgcg
    15421 atggtgctgg cccaggaaac ggcaattatg ctgctcgacg aacccacgac ctggctggat
    15481 atcagtcatc agattgatct actggaactg ttaagcgaac tgaaccgcga gaaaggctat
    15541 accctggcgg cggtgctgca cgatcttaat caggcctgtc gttacgccag ccatttgatt
    15601 gcattgcggg aagggaaaat tgttgctcag ggagcgccga aggagattgt cactgctgaa
    15661 ctgattgagc gcatttatgg tctgcgatgc atgatcattg acgatccggt agccggaatg
    15721 ccgcttgtgg tgccgctcgg acgaacggca ccttcaaccg caaatagtta aactaagtgg
    15781 tctgccatca tggcatcctg ttttctggat gccatcgcat agcgcaataa cacgctacca
    15841 caagccacca tcccgccgat cagcgcggaa aggatcacaa taatcgcctt acccggaccg
    15901 tcttttttca ctggcaatga cgggcttaac tgatatttaa acggcgtaaa attcacatcg
    15961 ttgacatttg cttttgttaa ttgctcgaca agatactgcc gattacgtaa ttcaccgttc
    16021 agttccgcaa cgtcagtaac cgctttttca atttccagtt tgcgttcaat accgtctgcg
    16081 ccgagggaaa tagagaaatc ggggtcatct ttaacggcct gaccattact gtatacgggc
    16141 tttttaattc ctgccgcgtt ggcaatgtcg agtgaataat tgaggcgctg aatgtttgca
    16201 tcaagttgat ttttcgtttt aatgcgatcc tgagccagtt tttctttttc aaactgggtt
    16261 ttgatctcca gtttattacg gacgttttct atcgactctt tcaccaccaa cgcagagata
    16321 taatcgatat acccgctcaa aacggtctgc gcctctttac tggttggcgc ggtaaaactt
    16381 agcgtccagg atgtatacag tgacggttca tcttttttct tactagcatt gtcatcaacc
    16441 gctttcattt tttcgctcaa tgcaacaata gcgcgatgca aatccagttc atcgattttc
    16501 gcctctttta attagtccat cacataaggt gatgaacgca ggtactcttc cagcaagcta
    16561 acagactgaa acttcttgat aaacaggtta aatgcaccgc cacggtcgat attaacatcg
    16621 agatcaagaa cgcggagttt ggtaaaggtt ttctccaggt cctgccactg aatggcttcg
    16681 gcgggagtaa taacggctga gctggtccat ttttgcggca ggatgaaaga gatcagtaaa
    16741 cctgcgcagg caaatgcaaa aacgaccgcc atgaccgttt ttttggcccg ccataaaact
    16801 tcgattagat taagtaaatc aatttcatta ttactgggcg acgccagagg atactcagaa
    16861 aaatgagcgt cacttccctg tttaatattc aatgatgaca tggggtataa cctgaaaatg
    16921 agtccaatag gggaaaatct ccacggcgct cactttacca gttggtgaga attatccgaa
    16981 gctgaagttt gtaaattcct tccactgaat gcaacattca ctctatttaa gagaaatatt
    17041 catcaaacct ataaatcaaa caaataaaaa acccataatt attttctgta att
//
LOCUS       DAETQL010000083        16913 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.083, whole genome shotgun sequence.
ACCESSION   DAETQL010000083 DAETQL010000000
VERSION     DAETQL010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.083"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..606)
                     /gene="capU"
                     /locus_tag="K0W65_003071"
     CDS             complement(<1..606)
                     /gene="capU"
                     /locus_tag="K0W65_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBI3096239.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHEF"
     gene            complement(609..1451)
                     /locus_tag="K0W65_003072"
     CDS             complement(609..1451)
                     /locus_tag="K0W65_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBI3096240.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            1637..2833
                     /locus_tag="K0W65_003073"
                     /pseudo
     CDS             1637..2833
                     /locus_tag="K0W65_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2916..3173
                     /locus_tag="K0W65_003074"
     CDS             2916..3173
                     /locus_tag="K0W65_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096241.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(3731..4498)
                     /gene="fecE"
                     /locus_tag="K0W65_003075"
     CDS             complement(3731..4498)
                     /gene="fecE"
                     /locus_tag="K0W65_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HBI3096242.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(4499..5455)
                     /gene="fecD"
                     /locus_tag="K0W65_003076"
     CDS             complement(4499..5455)
                     /gene="fecD"
                     /locus_tag="K0W65_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HBI3096243.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(5452..6450)
                     /gene="fecC"
                     /locus_tag="K0W65_003077"
     CDS             complement(5452..6450)
                     /gene="fecC"
                     /locus_tag="K0W65_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HBI3096244.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(6447..7349)
                     /gene="fecB"
                     /locus_tag="K0W65_003078"
     CDS             complement(6447..7349)
                     /gene="fecB"
                     /locus_tag="K0W65_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HBI3096245.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPTWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(7394..9718)
                     /gene="fecA"
                     /locus_tag="K0W65_003079"
     CDS             complement(7394..9718)
                     /gene="fecA"
                     /locus_tag="K0W65_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HBI3096246.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYVSYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(9805..10758)
                     /gene="fecR"
                     /locus_tag="K0W65_003080"
     CDS             complement(9805..10758)
                     /gene="fecR"
                     /locus_tag="K0W65_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HBI3096247.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(10755..11276)
                     /gene="fecI"
                     /locus_tag="K0W65_003081"
     CDS             complement(10755..11276)
                     /gene="fecI"
                     /locus_tag="K0W65_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HBI3096248.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            11574..11756
                     /locus_tag="K0W65_003082"
                     /pseudo
     CDS             11574..11756
                     /locus_tag="K0W65_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11750..11854
                     /locus_tag="K0W65_003083"
                     /pseudo
     CDS             11750..11854
                     /locus_tag="K0W65_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            12194..12451
                     /locus_tag="K0W65_003084"
     CDS             12194..12451
                     /locus_tag="K0W65_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HBI3096249.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
     gene            complement(12771..12902)
                     /locus_tag="K0W65_003085"
                     /pseudo
     CDS             complement(12771..12902)
                     /locus_tag="K0W65_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            13184..14542
                     /locus_tag="K0W65_003086"
     CDS             13184..14542
                     /locus_tag="K0W65_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HBI3096250.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(14781..16166)
                     /locus_tag="K0W65_003087"
     CDS             complement(14781..16166)
                     /locus_tag="K0W65_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBI3096251.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(16216..16563)
                     /gene="tnpB"
                     /locus_tag="K0W65_003088"
     CDS             complement(16216..16563)
                     /gene="tnpB"
                     /locus_tag="K0W65_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBI3096252.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(16560..>16913)
                     /locus_tag="K0W65_003089"
     CDS             complement(16560..>16913)
                     /locus_tag="K0W65_003089"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3096253.1"
                     /translation="RKGCPNYPPEFKQLLVAASCEPGISISKLALENGINANLLFKWR
                     QQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFRHGTLRFNGN
                     VSEKLLTLLIQELKR"
BASE COUNT         4088 a         4418 c         4480 g         3927 t
ORIGIN      
        1 aaattcgtgc cctttttccg ggcgcaacat tcctacctga gcaattacgg ggcatcctct
       61 tcgggacgcc agccaggaaa gaacactcgg aggcaaactg tttcgggaat caacgtataa
      121 ttcctggaaa tcaaagccag gaggcacaac agtaacccga gttcgacatc cttcctgctc
      181 cagatgtgtc ttcatatttg ttccgggaac aatcacctca tcgcaaaaat gatttattga
      241 aaaaaatttt gtttttcgtg tcaaatatgt cttttgcctg ataattctga atggatgttt
      301 ccaggtaaaa aaccgtacca aaccaacaat attactatca tgtccactgt gacaaacgat
      361 tgcatcgggc tgaaaactat gaactattcc gagtaatctc catacagtag gaatgtgaag
      421 actgtttcga aataacgcga atgtgatatc aattccaaac ttactggctt caaaagcaat
      481 tttgctgttc tccctgcaga caagcagaac tgaatgcccc attttcttca gggccttcat
      541 ttgagcaaca gcctgtaatt cctgaccacc tatattcggt gatgattccg taaatagtat
      601 attcattatt agtcatccgg taatcttggc cccttatgaa gggccagcaa cgatgaaaaa
      661 aacggtgttg tataataaaa aagtcgtctt ttcagccata cgcaattttc ccgttccttg
      721 gtgcttattc ttcctatccg tgtagttcct ttagcaggag cattcattct tctttctgtt
      781 gtatataaat aatggaatcc aagttcttca gcgatctgaa tataatcctt gttataataa
      841 ccttccggcc aacacaaatg tttactgcat tttcctgtca ttttttttaa atattctctc
      901 ccgctcaata agtcctgccg aagatgttta cactgttctt ctcgtgaagt gaacttttta
      961 tcccaacgtg tatgagtatg tgtatgaaca tgaaattcga ccagaccaga ttgcaacatt
     1021 tcgttcactt cagaccagcg aagcataaca ttatcagcat tcccggttgc tatttgatgt
     1081 tcgcaatcgc gatgagaata ttcctttcca ggagagtgac gaaccggacc atttccaata
     1141 aaactggtaa ttaaaaaaat atgtgccttg agattaaact cgtttaataa aggatatacc
     1201 tgaaaccagt tgtcaaggta tccatcatca aatgtcaaca taacgctttt acggggaagt
     1261 tttccgcccc gataaaaaaa ctccagttca tctgatgaca gtgttttcca gttgttttca
     1321 gccaaccatt tcatttgttt ccggaatgtc tcgggagaaa gtgtaaccag tcctgggcaa
     1381 tggctgacat gatgatacat taataccgga acatgatttg ctttaaacaa aatgccccct
     1441 ccgtttaaaa tttaaagttt ctggcaccca gtaagtagag ataaaaggaa agcctaataa
     1501 aggtggtgaa ctttatatac tactaaatca aacatcaaca atttataaac aataatatat
     1561 tatcatatct tttaaaaacg gctcactaag aaccttgagg tggcacacta aatttagcta
     1621 cctgaacaga ggtgatatgc tcacctcaga acaacacagg tgccataatg aaaaaagaaa
     1681 tttcagcgca gagtttaaac gcgaatccgc tcaactggtc gttgaccaga aatacaccgt
     1741 ggcagatgca gccagcgcta tggatgtcgg cctttccaca atgacgcgat gggtgaaaca
     1801 attacgtgat gagcggcagg gaaaaacacc aaaagcctcc cccattaccc cggaacaaat
     1861 tgaaatccgt gagctcagga aaaagctaca acgtattgaa atggaaaatg aaatattaaa
     1921 aaggctaccg cgctcttgat gtcagactcc ctgaacagtt ttcgataatc gggaaactca
     1981 gggcgcgtta tcctgtggcc actctctgcc atgtgttcgg ggtccatcgc agcagctaca
     2041 aatactggaa aaaccgtcct gaaaaaccag acggcagacg ggctgtatta cgcagccagg
     2101 tacttgaact gcatggcatc agccacggct ctgccggagc aagaagcatc gccacaatgg
     2161 caacccagag aggctaccag atggggcgct ggcttgctgg cagactcatg aaagagctgg
     2221 ggctggtcag ttgccagcag ccgactcacc ggtataagcg tggcggtcat gagcacgttg
     2281 ctatcccgaa tcatcttgag cgacagttcg ccgtaacgga accaaatcag gtgtggtgcg
     2341 gtgatgtgac ctatatctgg acgggtaagc gctgggcgta cctcgccgtt gttctcgacc
     2401 tgttcgcaag aaaaccagtg ggctgggcca tctcgttctc gccggacagc aggcttacca
     2461 tgaaagcact ggaaatggca tgggaaaccc gtggtaagcc cgtcggggtg atgttccaag
     2521 cgatcaaggc agtcattata cgagcaggca gttccggcag ttactgtggc gataccagat
     2581 cagacagagt atgagccggc gtggaaactg ctgggataac agcccaatgg agcgcttctt
     2641 caggagtctg aagaactaat gggtgccagc gacgggctat gtaagcttca gcgatgcagc
     2701 tcacgcaata acggactata tcgttggata ttacagcgca ctaagaccgc acgaatataa
     2761 tggtgggtta ccaccaaacg aatcagaaaa ccgatactgg aaaaactcta acgcggaggc
     2821 cagttttagt tgaccacaac agactacctg aagggagccg cggtcgccta gcagttgcag
     2881 tagcaggaga tcatccagcc gcagtacagg tcacgatgac tctggttaat gataccggct
     2941 ttgaccccgt attttccggc tctatcgctg aatcatggcg tcagcagccg tgcacaccat
     3001 cctattgttg tgactgggag gctgccacca tgcttcgcgc tttccctctg gcgaaaaagg
     3061 gagaaggacg ggcccgtctg ccttcacttt atgccagctt cggtaagctg ggtgagacac
     3121 cgactcatga agatatcatt gataacaatc gatccatcaa ctggcctgta taacgtggct
     3181 gccggtgatt aagaaagctg cacctaccta agtagtagca aacgcacact ttttagaaaa
     3241 atcgatggtc agaaactgga ttagcaattc cgttccaggg ttgcttttga tttacgttgg
     3301 cgtctgatca ttgatttatc ctcaaaagcc caacctcatt ggtaatgaac cagctccgtg
     3361 aatgtccgct ctggcacaga gcgaaatttt ttgatctccc cccctgaaat ctaaacttag
     3421 tcatgtcacg tttttgggtt tctaaaattt taacttcgcg tttttcgttg ccgtaagggt
     3481 tatacagaaa tgtccgttaa gcagagttca aaattgattg ccgtgatcac gactggtttg
     3541 aaagccgcgc ccaagcctgt acagctctgg tttgcgttga ttatgaacct gtcagcctaa
     3601 agcaagcgga tggacgatga gtattggtaa tctttcagag tccggaaaag ttcagcccca
     3661 gtctgaacag gcttgctggc gccagtccag tttcattcag tcgtggtttg gttcttacgg
     3721 cctgtgcaat ctacctcatt aggcacatcg gcctgccaga taccggctcg gggtgtattt
     3781 ccgcttccac gctgaatact gttctcagca atcctggggt catcacctct tctggtgtgc
     3841 cttgcgccat aacatgtccg tttgccatta ccaccagttg atcgcagtac cggctagcct
     3901 gattaaggtc gtgcagcaca gcgaccaccg ttttcccctg agtccggagt tcgcccatca
     3961 accgcatcag gtccacctgg tgattgatat caagataggt ggttggctca tcaagtaata
     4021 caacgggcgt attctgggcc aggaccatcg ccagaaatgc gcgctggcgc tgaccgccgg
     4081 aaagctcggt taaccgacga acggcaagat gattgatccg ggtctggttc atggcgacat
     4141 taactcgtgc attgtcttca gcggagagac gcccccagag tgacagccag ggattacgac
     4201 catacgaaac cagctcctgg actgtgatcc cctctggcgt taaatggtgc tgaggcagca
     4261 gcgaaagcct gcgggccaac tggcgtgatg agagcatatt tatgggatta tcgccgagaa
     4321 atacggtgcc agactgcggc attaaaagcc gcgaaaaaca gtttaacagc gtcgatttcc
     4381 cgcaaccgtt agggccgatc agggcggtga tcttccccgt tggcagtgag agtgaaacgt
     4441 cgttaagtac cttgtctgtc ccgtaactga ccgtcagatt ttcagttcgt aaagtcattt
     4501 atcgcattct cacaagcaac cagacaaacc acggcgcacc gataatggcg gtcagcacgc
     4561 caaccgggag ctccagtggg ggatgaataa ttctcgccag cagatcggca accaccaaca
     4621 gcaacgcacc tgtcagggct gaaacaggca gcagtctgcg gtgacgtcca ccggtgatgc
     4681 tacgcatcat atgcggcacc acgagaccaa taaagctaat cgggccgcag gcggccacgc
     4741 cggtagatgt catggcgaca gctagtaaca aagcccagaa tcgggtatgg ggcaccgaca
     4801 caccgagcgt ggtggcgcgc gcatcgccga gtgcaaggag gtcgagatcg cggcaaaaac
     4861 tcaggctcag cggcagaaat aaaatcatca gcgggatggc aatcttcaca aagctccagt
     4921 cacggcccca taagctgccg gtcagccaca gcagggcgtt gttcacatcc tgcgggcgcg
     4981 agagcatcag ataatccgtc aggctggccc agcatgcaga aagcgccacg ccggtgagcg
     5041 ccagcttcat cggctggtgg gtctttgcca gcatcttcag taatatcaac cccgccatgc
     5101 cgcccgcaaa ggccagcagc ggcagcacca tcacgggcag tgacggcata agaagtagag
     5161 cccccacaga ggccaggctg gcggcatggt taacgccgag aatatccggt gatgccagag
     5221 ggttgcgcac aatcccctgt atcagcacgc ccgccacggc gagggctgca ccgacaaaca
     5281 gtgccagcag caagcgcggc agtcggtact ccatcaatac ataataatgc tcgcgtccgg
     5341 cctgccagtc ggtcagcagc gcgcgccacg gcacggggat cactcccata tggagtgata
     5401 acagcgcaca gcccgccagg gcaagggtga tgaaaataac cagcgcaatt ttcatcctcg
     5461 cctcctcaca agccagacaa agcaagggct gccaatcagc gccagcactg cgcctgcggg
     5521 cagatctccg gggaaggcca gcgcgcgtgc gagtacatct gccagcagca tcagcgtggc
     5581 ccccagcagc atgctcactg gcagtacgtt gcgctgatcg aagcctgccc agaagcgcgc
     5641 cagatgtggc accagcagac cgataaacgc caccggacct gccacgctga cgcacgcacc
     5701 aaccagaagc agcactaaca tattgatgac caaacgtagc ctcgtcaggt tcactcccag
     5761 cgtatgggcg gtgctgtcgc tgaggttgag caggttcagt tgattcgcca gcagtaacac
     5821 gacagggact gcagtgacca ccaccggcaa gagctgccag acatcctgcc agcgggcgtg
     5881 ggacactccg cctgccagcc agtaaaagat gccgtaagca tgatcttcgg ccagcagcag
     5941 ggtgatgcgg gtaaggccca tacaaaaggc cgacagcgcg atacccgcga ggatcagttt
     6001 gtttctgtca tgggtatgac gaaatccgcc tcctgcggtc atgaccagca gccagctcac
     6061 gccgcccccg catgccgcga tgaacgacag agaatagcct gcaatcggcg tcggactcag
     6121 cgcgctggta agcgccatag ccagcgccgc gccgctgtta atgccgagca gtgaaggaga
     6181 ggccattggg ttgtgggtca gggtttgcag cagcgtgccc gcgagcgcca ggcttgcgcc
     6241 gatcagaacg gcgaccaggc ttcgtggcaa acgaaggttt tgcaccagcg cttctggtag
     6301 cgttggcgtg tgtccaggca gcagggcgcg ggttgcatct gctccggaaa caggaatggc
     6361 cgagtagcaa aacagactca gccagaaaat aataataagt gctgcaacgg gaagccccca
     6421 cagcagcatc gggtgtttta tcgcggtcat ttcacaacgg taagcggctg atggtggaag
     6481 atttttaccg tgtcagcggc aatacgctct gcagcaaaaa taccgcgcat ccgcgcccag
     6541 gtgttactgt cgaccgaagc aacctgctgc ttctgcgcgg cggttaacat ctgccagagc
     6601 ggatcttgtt gccagcgttt aacaatgctc tcttcgcgat agtgggcaac cagcagccag
     6661 gtaggattga ccgccagcag ttgctccagg ccgatggacg gcatggacga acccgccatc
     6721 gcagcgggaa cgttcagccc cagagaggcc agcacgctgc cggtccaggt ctcctgagta
     6781 tgcaggttga attgctgttc gcgtgatgtg ccaaaggcca cgcgtgtccc tttgggaagc
     6841 tggctggccc actgcgccat cctctcttta tgttgttcca gacgtgcctg catctctcgc
     6901 tttttaccca ccatttcgcc gatgatagcc gcagattgca aattttcagc gtaggtttcg
     6961 ttgcgggact taagcagcag taccggcgcg atttgctgca aggcgatgta aacccccgca
     7021 tggcgactgc tgtcggcaat gatcaggtct ggtttcagag cggcaatggc ttccaggctc
     7081 ggctgcgcgc gcgttccgac ggactgccac ggtttcaggt gcgcacgcac ttcgggcagg
     7141 atgcgttttg catcgttatc gtcggcaata ccgatcgggc tgacgtccac ggcggccagc
     7201 gcatcggcga acgagagttc cagcaccaca atccgttgtg gcgttttttc gagtgtaaac
     7261 gtgccgtgtt cgtcctgaac catggcggca aaggcgtggc tgatcaccag cagcaggcct
     7321 gcaaaaagaa aacggataaa tgccaacata atcacattcc agctaaaagc ccggcaagcc
     7381 gggcgttaac acatcagaac ttcaacgacc cctgcatata cagcgtgcgc ggctggcctg
     7441 catagatgcc tttgttgttg tcgtcataag agcggatgaa gtagtcctgg tcgaagatgt
     7501 ttttcacacc gaatgccagg ttcagatctg ccatctgcgg gccaaagtca tacgccacgc
     7561 gtgcacccca gagcatgaag ccgggaatgc ggccggtact gccgtcggcg ctctctttca
     7621 ccgtattggc gttatccgca aactggctgg actggaaatc gctgttcaga ttgaacgtcc
     7681 agtttcctgg cttgtagtcc acgcccagcg tgcctttatg tttcggggag aatggtacca
     7741 gattgccgta ggtgtcgcct ttctcgcgga tttccgcgtt cacatacgca tagctgacgt
     7801 agatggaaac gttatcaagc gttggcgtta gcgtacccag atcgtaacgt gcctgcgttt
     7861 ccagcccggt atggcgcgtt ttgccacgtg cagtgacggt gtcgttggtc tggttggagt
     7921 cgtactgatt gttaaagtta atcaggaaca gccccatttc cgccgtcagc gcgccgtcgt
     7981 cgtagcgggt accgagttcc caggttcgcg ctttttccgg ttccacattg ccgctttgca
     8041 cagccttgcc aatctggctg tactgtacgg tgccgaacga gccttcagtg tttgcataaa
     8101 gattccagct gtcagtcagg tgatagagca cgttcaacgc cggaagcggt gcgttatagc
     8161 tcacttcttc gtgcgtgcct gtgatggcgt tgttctggta tgactcgata tgttcgaaac
     8221 gcatacccgg cgtgatggtc cagttgccga tgtcgatttt gtcatccaga taccaggcgt
     8281 gcgcctcggt gccggaacgc gtatcgcggt cgtaagggct tgagccggac ggcaactgcc
     8341 cgctgctggt ggcggtgtag taacgcattt catgcgttga ttcattcaca tagcgatagc
     8401 ccacgcccac ttcgtgcgcg gaagggccga tcataaagat ctggctgtag cgtggctcaa
     8461 taccgcgcac ccagtagtta cgcggcgaga gggtgatgcg tttgccttgc tccaggtagc
     8521 cgctgcgcag ggtttgggtg tagaacccct gaatgttgaa tttatgctgg ctgtctggct
     8581 ggaactggta gcccaggctc gccagcttgc gacgacccca gaagcggtca tacgggcggg
     8641 tggattgcca gcgatcggcg tcgtaatccg cgcgagacag gccaccgggc atgtcggctt
     8701 caccgtcgta atattgcagc aggctgttga aggtgtgcac ctcatccggc gcatatttgc
     8761 ttttcagcat caggtcgtcg atgcgggtgg cgctgtgctc gcgccagtca ctgccgcgcg
     8821 tgccggagta gagcagcgcg gtgccaaaac cgttgtccgc tgtgccgccc accatcaggt
     8881 tgtgcgtctc tttcgggttg ttttgtgaag aggttgggct gagctgacct tccacgcccg
     8941 cctcgatacc aaagtcctgc ggaatggcac gggtaacaaa gttcaccacg ccgcccacgc
     9001 tctgcggtcc gtaacgcacc gcaccaccac cgcgtaccac gtcaatggca tccatgttgc
     9061 cgagcgaaac gggagccagt gaaagctgcg gctgaccgta aggggcgaag gggacgggga
     9121 tgccgtccat caggacggtc gagcggctgg cgaggcgcgg gttcaggccc cggatgccaa
     9181 agttcatcgc caggtcgtgg ctgccggtgc cgttgttttc cggcgcgctg acgccaggga
     9241 tgcggttaag tacctcacgc atggtggttg cgccggtttt ggcgaaatcc tcacggcgga
     9301 tcacgtcacg cgcgccagca tgttcaaata cgtcgttttc acgcgcatca cccagccagt
     9361 cgccgaccac ggtcagggca tcttcttttg gtgcgggcgc gggctccagc gtccagctgt
     9421 tatttcccag cggttttacc tgcagtccgc tgccgtccag cagttgttgc aggccgctct
     9481 cgacgtcgta atcgccgtgc aggccgttgc tctgcttgcc gcgcgtcagg ctggcgtcaa
     9541 ccgagagggt aaatccgctg tgtgcggcat actgattgag cgctttatcg agcgatcccg
     9601 gtgcgatatt aacctgtgca gcaaaagcgg aaaacgagag accggccagc ggcagcaggc
     9661 tcaggcgaat ggcgttaacc aaaggtgttg ttttacgaaa aacgcgtaac ggcgtcatac
     9721 cttccccatc atcatttttg ttgtgttcag ctatgagtcg aacgagaagg gtaaaaagga
     9781 caatcgaaat aagaattatt ttccttacag tggtgaaatg tttatccagt accgcgtaat
     9841 agactgaatt ttaacgggaa gcgtttgcgc gataacgttc aggatcgcat cggtattttt
     9901 cagcgggaac gtcccgctca ggcgcagccc ggcaacggcg ggatcgcagc gcagcacgcc
     9961 gttgcggtaa cgggttagcg tggctatcac ctcacccagc ggtttatcgc tgaagctcag
    10021 gatgtccttc gtccagcttg tactctcgtc atccagcggt ttcactgcgc caaactcaga
    10081 ggcgctgaac tgcaggcttt caccagcgtt cacgatgcgt ttttgcgcgg gggcactggc
    10141 gagaagcact tccacagcgt gctgctgaac gtcaagctgc gtgaaattat cctgctggcg
    10201 gacggtaaat tctgtcccta aagcagtgag ctggccctga cgggtcagga cacggaaggg
    10261 gcgttgcagg gcatctttcg cggtagtaat ggcgatttca ccgtaccaga gccggacggt
    10321 gcgctgatgc gcatcaaaac gcacatccgc cgcgctttgg gtattcagcg tgagcaggga
    10381 gccatcttcc agttgctgac ggctgactgt gcctttggcg gtgcggtaat ctgcccgcag
    10441 accttcgccg gtttccgact gccagagctg ccagcctccg ccagcgccga gcaacagcag
    10501 taatcctttc atcacgtgac ggcgggtgag gcgggtatcg tgcaacgccc ggctggcaac
    10561 gtcgccaggc acaccgccaa gctggttgcg caggttttca acctgctgcc aggcccactg
    10621 gttatcctga tcctgttcat accactgttg ccagcgcgct tcctgttgtg ggctgacgcg
    10681 ctcgccgctt agcacggcat accagtggga agctgaacgc agcgcctgac ggcgggaatc
    10741 ggttaacaaa ggattcataa cccatactcc agacggaaca gcaggcagtg ctcgacggct
    10801 ttcgccacgt attttttcac ggagctgatg gaaacaccga gtttgtgcgc aatctcgctg
    10861 tatgtcagac catccagttg cgaaagcaga aacgcttcac gtgttttgcc gtttagcccg
    10921 tccagcatgc tgtcgaggag ttgtagggtc tcgagttggc tttcgcgttc ctcaggtgaa
    10981 ggcgctcccc cctccggcat aagcgccagc atctccagat acgctttttc cagcgcgttt
    11041 cggcgaaaca ggtccaccat cacgcgtttg gcgatagtgc agaggaagga gcgaggatcg
    11101 cggatcgtcg agagcgtttc gctgaccatt acccgcaaaa aagtgtcctg ggcaatgtca
    11161 tctgcatcaa aagcagactg gagtttgcgc gtcagccagc ttttcaacca gccgtgatgt
    11221 gtgccataaa gcgactcgaa cgttaaggaa gctgtggtag tggcgcggtc agacatgcgg
    11281 agtgcatcaa aagttaatta tcacgtagtc atattaatat gagaatggtt atcattacaa
    11341 ttggaaataa aattgtttcc aatagacatt tttaacatgt tgtttttcta agtgttataa
    11401 ggtaggtata aaatgggatg gagcctctgc ttctgacatg tgtcggtcag aatgactcat
    11461 gatgtggtct gctattattg acatcctcac tgccctaaag gatggggatt tcggtaatga
    11521 tcttacccag caatagtgga cacgcggcta agtgagtaaa ctctcagtca gaggtgactc
    11581 acaagacaaa acagtttcaa ccagtaaaaa aacgtaaaca ccattcgcat gaatttcgta
    11641 gttaagatct gaagcttgca gaatgcatcg gtgttactgc cggagtccgt gaacttagtc
    11701 tgcatgaatc accacagctt tacatctgtc gcagtaaaca gcaaaatcag caaacttact
    11761 taattatatc gagtgcgatt acaatcgctg gcgatggtac agttggtgtg gcggtctcag
    11821 tccggaacaa tttgaaaatc ataatctcac ttaaaacggc atccatttta caaaagatgg
    11881 ccgtctgtgt atttgtaatg aataccttct acatgtttag agtgcataat ccacgacaag
    11941 gcgatatcct aaaaattagc tagcaggcaa ctttgatggc atagataatg tttttagtaa
    12001 aaaagagttg actgaatata atacagcctc ttcctacata agaaggaatg tgctcacaaa
    12061 aaaaaaccat tagtctgctt cctgttctta caataatcag tattatgtat tttatttata
    12121 tagcaaaata tgcttgttac acctgtcata attaggcatg gtaaaattat tgactgaacc
    12181 ctgggggaga aagatgcaca gtgattatta taatatggtt tttggtgaaa aattggccaa
    12241 tattttatat gaagctaact ctcaattttt ttacgagaga aatgtcatag aagaggcagt
    12301 caacgcttta ttctgtgagc gtgaaataat aaacaacaag aacataataa agaagctgat
    12361 gttctttttg tcagatgtta atcacaccaa gaaagatgtt gttcagagcg cacttaatat
    12421 tatcattgac attacatcag gtgatatttg agattttaca taaaagataa tctctcaagg
    12481 agtcttatct gaaaacgaaa tatatagtcc ctctcgtttt gtaatatcga ttggctttgt
    12541 tgaatcggtt acgtaccccg gagccggtca tattttcagg taatacacac gctttcagac
    12601 atttcggctt tcgtcatttt tgtatcatgg gcatagcttc tgctacctga ccatcataat
    12661 cacgcagctt cagtgaagca gtgaccacct gtttaacccg gtgtatcgtt atgatcttat
    12721 ccagcaattg aaccgccccg ggtttcctgg agagtgtttt atctgtgaac tcaggctgcc
    12781 agatcatcgt ttccgatgga agcataataa gctttttctg cttctgccgg tgggatatgg
    12841 ccctgccttt ccagcaatcg tcgattgtta taccagtcca cccacgtgag tgtggtcagc
    12901 tcatcatact gacacgtgca ttacccctac cgcgataatg caaccgtaca atatagggcg
    12961 tttcctaaac gcagccctca tcactccaat ccaacaagac acagcatttt tgatcacgaa
    13021 gaaagacaaa taatttccgt aaatgtttcc gttgttacaa ataacaacag tgttttacat
    13081 ttctttggcc tcaggcccgc gcatgttgcc attcgatgtc attttcctga ctcgtttctt
    13141 ctttatggtg tagtgtgatc ttcactttat agatgctaac aatatgaaaa gaaaaattat
    13201 tcccgtgcta atcggttgta cgctctcttt ctctgccctg gcggcacaac ctacggctga
    13261 gcgctatgtc gtcagttttc cagaaggcac gcacgtgaac tacgccggcg catttgccag
    13321 tgcattcccg aacggccttc cggttgggat aggttctggc ctgctgttca ccggtaaaca
    13381 gggcgatgcc ctgacattcg caaccattac cgatcgcggt ccgaacgcgg attcaccgaa
    13441 agaggggaaa aacgaaacta aaattttcgt tacgccagat ttcgcgccgc tgctgatgac
    13501 gattcgtgta caaaacggca aagcggaggc catcgatccc cgtcctctgc atgacgataa
    13561 aggcgcgatc aacggtctgc cgctggcaag cgacgtgatt ggctccacca atgaagtggc
    13621 cttcagcgac acgttacata gactgaaggg ggataatcgc gggctggata cggaagggat
    13681 cactccggat ggtaaaggag gttactggct gtgcgatgag tatggcccgt tcctgattaa
    13741 catcgacagt aaagggaaaa tcctggcaat ccacggtccg caggcggcgg aaggggaaaa
    13801 agcgattgcg ggcggtttgc caaatatcct caaatggcgt caggcgaacc gcggctttga
    13861 aggcctcacc cgtatgccgg acgggcgtat tattgtcgcc gtgcaaagta cgctggatat
    13921 cgacgctaag agcaagaaaa aggcgctgtt tacgcgtctg gtgagcttcg atccggcaag
    13981 cggaaaaacc gcgatgtacg gttatcctat cgacagcgcg gcctacagca aaaacagcga
    14041 cgctaaaatc ggagacatcg tggcgctcga taaccagcac atcctgctga tcgaacaggg
    14101 aagagataaa aacaacagaa tgcgtaacct gatttacgag gtcgatctca ataaggcgag
    14161 cgacctgtca ggcttcgaca agccgggtga gtatccggag tttgatgatg agaaaaccct
    14221 gtcacagcgc ggcattaccc ttgcgcagaa aacgcaggtt gttgatctgc gctccttggg
    14281 ctggcagcag gagaaggctg aagggttggc gctgatcgac agcaaaacgc tggcggtggc
    14341 gaacgataac gactttggcg tgaaggtggc gatgcaacat ccggtcgagg gcaagacatt
    14401 caaggattat cgggtgaacg cggaaggaaa gctgacgctt gacgacaagc aggttgaaac
    14461 gaccctcaga gtgaagccac tggagaaacc ggagtctgac agtgaactgt ggattgtgac
    14521 gctgccggaa gcgttgaaat aagtttataa ggtacggaca gcagtatcgc ttagtttttc
    14581 aaattcatgt atgtgaacaa tattggggtt ccggcgtaga cccccaatga aagcgtcacc
    14641 acgtgaaaac atgcctgcac ggtttacacg tcatcccatg gtggcagccg gttaactttc
    14701 ctgcgccacc agtggaaaaa ggccataagc agtccgaaca aaagtaagcg tacagcgagg
    14761 gccgtattga cggggatgtg ttattcagct ggcagtgcta tgcgccacgg aagcagttcg
    14821 ctgacccggt tgaccggcca gtctgctatg acgccaagca catggcgaag gtagctttct
    14881 ggatccacgt cattcagttt gcacgtcccg atcaggctgt acagtagcgc tccccgctca
    14941 ccaccatggc cagagccgaa gaacagggtc ttcatctttt cacgcaacca gctttccagg
    15001 gatttcaaca gcggtttcgt ttttcgctga cgttcagcaa gccgctgctc tgccggcatt
    15061 ccccttatat ccgcctctat ggcgtacaac tgaccgatct gctccagggc ttcttccgtc
    15121 agtgctgacg ggatgcggac gtgcacatcg tggatctttc ggcgggcatg agcccagcag
    15181 gcagcttccg ttatcccacc attgcgatac agctcgttga acccggcgta cgcatccgct
    15241 tgcagcacac cgctgaagca agcaagatga gtctgcggat ggatgctttt tctgtccggg
    15301 ctgtaagcga accacactgc aggtgccaac gctgacccgg cattgcggtc atcacgaaca
    15361 tacgcccaca accgcccggt cttcgtcttc ttattacccg gcagcagtac ctggaccggg
    15421 gtatcatcgg catggagttt gccgtcagtc atgacatagc catgaagcgc ctcttccagc
    15481 ggagacagca gccggcagca tgcatccacc cagcccgaca gcagtgaacg gctcagctcc
    15541 acaccttgcc ggccgtatat ttctgactgg caatacagcg gggtgtgctc tgcatacttc
    15601 gaggtcagca cgcgggccag cagccccggt ccggcgatac cccgctcgat gggccgcgaa
    15661 ggtgcaggtg cctgcacgat ggtatcgcac tgagtacagg catgtttttc ccgtaccgtc
    15721 cggataaccc ggaaggcgct acgcatcaac tccagctgtt cggcggtatc ctcgcccaga
    15781 tagctcagtg aaccgccgca gttcgggcag cacggcgccg caggcaacag tcgcttttcg
    15841 tcacggggta gtgattcagg gaacggctta cgggtgcggg tctgacgcaa cggacgctgt
    15901 actgccgggt catacaccct accagtcagc gtatcgctct ctttctgaag ccggttcaga
    15961 tcggcttcca tttgtgcgat acggcgggag actttttcgg aacgactgcc gaagttcatc
    16021 cggcggagtt tatccagctg cgcctgcaga tggtctattt cgcgctcccg gttgctcagc
    16081 ttttcctgca gggcgtggat cagcgcttcc tgttcggcca ggcgctgttt cagcaggaag
    16141 atgtcgtcag aagagatgtc gttcataagc ccgtatttta ccgggcttat tctgtgacaa
    16201 ccaggataaa gagatttaca gcatggtcag ggaggtcagc agccgcttgg gctgtcgcca
    16261 gtcgatacct tccagcagca tcgccagctg cgcctgcgta aggaacactt tgccatcacg
    16321 ggctgacggc caggcgaagc gcccacgctc cagccgtttg gtcaggaggc acagtccgtc
    16381 gccggtggac cacagcagtt taacctgact gccgctgcgg ccccggaaaa tgaaaatatg
    16441 gccggacatg ggatcgtctt tcagcgccgt ttgtactttc gcagccaggc cgttgaagct
    16501 atttctcata tcggtgatac cggcaaccag ccaaattttg gtcccggaag gtaacgggat
    16561 catcgcttca gttcctgtat cagcagagtc aggagctttt cgctgacatt gccattgaag
    16621 cggagcgtcc cgtgccggaa cgttacctca cagctgatac tgagggtttc cgggtcctct
    16681 gcgagcgatt ctggctgttc ggcagctgca tcgagagtca caggaagtag ctgggggctc
    16741 tctgaagaag gtaatagcag ctttccctcg cgccattgtt gtcgccattt gaacaacaga
    16801 ttggcgttaa tgccattttc aagagcaagt tttgagatgg atatcccggg ttcacaggag
    16861 gcagcaacga gcagctgttt aaattcggga ggataattag ggcagccttt tcg
//
LOCUS       DAETQL010000084        16393 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.084, whole genome shotgun sequence.
ACCESSION   DAETQL010000084 DAETQL010000000
VERSION     DAETQL010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.084"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(91..2067)
                     /gene="speA"
                     /locus_tag="K0W65_003090"
     CDS             complement(91..2067)
                     /gene="speA"
                     /locus_tag="K0W65_003090"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBI3096254.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2076..2207)
                     /gene="yqgB"
                     /locus_tag="K0W65_003091"
     CDS             complement(2076..2207)
                     /gene="yqgB"
                     /locus_tag="K0W65_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBI3096255.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2343..2558
                     /locus_tag="K0W65_003092"
     CDS             2343..2558
                     /locus_tag="K0W65_003092"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096256.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2862..4016
                     /gene="metK"
                     /locus_tag="K0W65_003093"
     CDS             2862..4016
                     /gene="metK"
                     /locus_tag="K0W65_003093"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBI3096257.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4453..5847
                     /gene="galP"
                     /locus_tag="K0W65_003094"
     CDS             4453..5847
                     /gene="galP"
                     /locus_tag="K0W65_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HBI3096258.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5924..6421
                     /locus_tag="K0W65_003095"
     CDS             5924..6421
                     /locus_tag="K0W65_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBI3096259.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6516..7223
                     /gene="endA"
                     /locus_tag="K0W65_003096"
     CDS             6516..7223
                     /gene="endA"
                     /locus_tag="K0W65_003096"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBI3096260.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7303..8034
                     /gene="rsmE"
                     /locus_tag="K0W65_003097"
     CDS             7303..8034
                     /gene="rsmE"
                     /locus_tag="K0W65_003097"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBI3096261.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8047..8997
                     /gene="gshB"
                     /locus_tag="K0W65_003098"
     CDS             8047..8997
                     /gene="gshB"
                     /locus_tag="K0W65_003098"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBI3096262.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9106..9669
                     /locus_tag="K0W65_003099"
     CDS             9106..9669
                     /locus_tag="K0W65_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBI3096263.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9669..10085
                     /gene="ruvX"
                     /locus_tag="K0W65_003100"
     CDS             9669..10085
                     /gene="ruvX"
                     /locus_tag="K0W65_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBI3096264.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10261..11241)
                     /locus_tag="K0W65_003101"
     CDS             complement(10261..11241)
                     /locus_tag="K0W65_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBI3096265.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11259..11963
                     /gene="yggS"
                     /locus_tag="K0W65_003102"
     CDS             11259..11963
                     /gene="yggS"
                     /locus_tag="K0W65_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBI3096266.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11981..12547
                     /gene="yggT"
                     /locus_tag="K0W65_003103"
     CDS             11981..12547
                     /gene="yggT"
                     /locus_tag="K0W65_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBI3096267.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12544..12834
                     /locus_tag="K0W65_003104"
     CDS             12544..12834
                     /locus_tag="K0W65_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBI3096268.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12842..13435
                     /locus_tag="K0W65_003105"
     CDS             12842..13435
                     /locus_tag="K0W65_003105"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBI3096269.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13428..14564
                     /gene="hemW"
                     /locus_tag="K0W65_003106"
     CDS             13428..14564
                     /gene="hemW"
                     /locus_tag="K0W65_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBI3096270.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14878..15841
                     /locus_tag="K0W65_003107"
                     /pseudo
     CDS             14878..15841
                     /locus_tag="K0W65_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15886..16389
                     /locus_tag="K0W65_003108"
     CDS             15886..16389
                     /locus_tag="K0W65_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HBI3096271.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4062 a         4221 c         4125 g         3985 t
ORIGIN      
        1 gaaaagcagc tagattattt ttaagaatat ataaaaagaa agcgggccga tttacgtaaa
       61 taaatcgacc cgccagcgga ataatcttaa ttattcatct tcaagataag tataaccgta
      121 caaacctgcc tcgaactctt caaggaactg ttgttgcagt tcagcatcaa gatcggtttt
      181 cttcacttga tcgcggaact gggttaacag cgttttcgga tcgagctgta catattgcag
      241 catgtccgcc acggtatcgc cttcgtcaga cagttctact tctacgctac cgtcagggaa
      301 cacgaacacg tcaactgctt cggtatcacc gaacaggttg tgcatattgc caaggatctc
      361 ctgatatgca ccgaccataa agaaaccgag catcggcgga ttctctggat cgtactccgg
      421 cattggcatt gtcgtggcaa taccatcacc atcaatatag tggtcgatag caccgtcaga
      481 gtcacaggta atatccagca gcacagcgcg acgttccggc acttgatcca gcccttccag
      541 cggtagaacc gggaacaact ggtcgatccc ccatgcgtcc ggcattgact ggaacagcga
      601 gaaattgacg tacattttgt ccgccatacg ttcctgcagt tcgtcgatta tcggacgatg
      661 cgcacggttt tgcggatcca gttgcttctg cacttcatgg cacatgctca gatagagttg
      721 ttctgcccat gcacgttctt gcaggctaaa ggtgccggaa gagtagccga tatgaatgtc
      781 gtgcagatcc atctggctgt cgtgtaacca ttcacgcaaa gaacggcgag ttcccggttc
      841 gtgcatttcc tgccaggttt cccacatgct ttgcagcgca cgcggcgcat cttctacggg
      901 cgcggtcggc accgtatatt cgttacgttc cacgccgatg atattcgaca ccagcacggt
      961 atggtgcgca gtcaccgcac ggcccgattc ggtgattacc gtcggatgcg gcagaccatt
     1021 ttcttcacac gcatcgccaa tcgcccagat gatgttgttg gcgtattcat tgaggccata
     1081 gtttacggag cagtcagact gcgaacgggt gccttcataa tccacgccca gaccgccgcc
     1141 gacgtcgaag cactgaatat tgacgcccag cttgtgcagt tccacataga aacgcgcgga
     1201 ttcacgaacg cctgtcgcga tatcgcgaat attcgccatc tgcgaaccga ggtggaagtg
     1261 cagtagttgc aggctgtcga gacgcccggc ttcacgcagg gtttcaacca gttgcagtac
     1321 ctgagtcgca gccaggccga acttcgattt ttccccgccg gaggactgcc atttacccga
     1381 gccctgcgaa gccagacgtg cacgcacgcc cagacgagga acgacattca gacgttctgc
     1441 ttcatccagc acaatggcga tttctgacat cttctcaatg accagataga ccttgtgccc
     1501 catcttctcg ccaattaatg ccaggcggat atattcgcgg tctttataac cgttgcagac
     1561 gatgacgcta cgagtcatgc cagcatgtgc cagcactgcc atcaactcgg ctttggaacc
     1621 ggcttccaga cccagcggtt cacccgaatg aatcagggac tcgatcacgc ggcggtgctg
     1681 gttaactttg atcggataaa caaggaagta atcgccgtta tagccgtagg attccctcgc
     1741 acgtttgaac gcggcgttaa tggaacgcaa acggtgctgc aggatctgtg ggaaacagaa
     1801 cagtgcaggc agacgctgac cctgtgcttc acgagttttc actaactgcg cgagatcgac
     1861 gcgagcttcc gggacgtccg ggtccgggca cacgctgatg tggcccagct cgttaacgtc
     1921 atagtagtta ttgccccacc aggcaatatt gtaagtacgc agcatcttgc tggcttcctg
     1981 ggagctcatt gcaacctcct gcatggagcg tagtacaccg tgttcgcccg ctgacgaagg
     2041 caaacccata gacatgtcgt cagacatagc gaacctcaaa ttattttatt aagtgtaaaa
     2101 cagttaacga ctgtcgcagc ctggaactgc gataacaacc cataagcaca tggattttcc
     2161 agcagtgtat gctgacgctc cgactgcgca accggtttct ttttcataac attattaagc
     2221 acataaccga acgtaagtgt gacagttcgg cgaaaccacg agaaaactct tgtttttaca
     2281 agagcgccct tgttcagtcc tcagtaactg taaccagctc ttgaatcctg agaagcgccg
     2341 agatgggtat aacatcggca ggtatgcaaa gcagagatgc agagtgcggg gaacgagtct
     2401 tcaccagaac ggtgagacag gttaagcagc agacaacggt tcattatttc gtatcacctc
     2461 cacggccgcc tgttaagacg aacccacaag ccaaaactct gatttcaacc cggctggaag
     2521 tggcaacacg aaagaaacgt cgtgtgcttt ttatttaagc cgcgcgccgc gttttatacc
     2581 ccacaatggc agaaaattgc aaaagataaa tacgcagaat gccggcattg tcaggaaaaa
     2641 tttccagcca cgtttttaac agaatgagac acgattcaaa aaaaagtgga aatcgggtga
     2701 agaattgacc taaaatagcc atccagatgt taatccatcc ataccgatta acactcagac
     2761 tgccagtgtt tttaacctgc agagtcgtgg taggatccgc taccacagaa aatccacaca
     2821 acagtttgag ctaaccaaat tctctttagg tgatattaaa tatggcaaaa caccttttta
     2881 cgtccgagtc cgtctctgaa gggcatcctg acaaaattgc tgaccaaatc tctgatgccg
     2941 ttttagacgc gatcctcgaa caggatccga aagcacgcgt tgcttgcgaa acctacgtaa
     3001 aaaccggcat ggttttagtt ggcggcgaaa tcaccaccag cgcctgggta gacatcgaag
     3061 agatcacccg taacaccgtt cgcgaaattg gctatgtgca ttccgacatg ggctttgacg
     3121 ctaactcctg tgcggttctg agcgctatcg gcaaacagtc tcctgacatc aaccaaggcg
     3181 ttgaccgtgc cgatccgctg gaacagggcg cgggtgacca gggtctgatg tttggctacg
     3241 caactaatga aaccgacgtg ctgatgccag cacctatcac ctatgcacac cgtctggtac
     3301 agcgtcaggc tgaagtgcgt aaaaacggca ctctgccgtg gctgcgcccg gacgcgaaaa
     3361 gccaggtgac tttccagtat gacgacggca aaatcgttgg tattgatgct gtcgtgcttt
     3421 ccactcagca ctctgaagag atcgaccaga aatcgctgca agaagcggta atggaagaga
     3481 tcatcaagcc aattctgccc gctgaatggc tgacttctgc caccaaattc ttcatcaacc
     3541 cgaccggtcg tttcgttatc ggtggcccaa tgggtgactg cggtctgact ggccgtaaaa
     3601 ttatcgttga tacctacggc ggcatggcgc gtcacggtgg cggtgcattc tctggtaaag
     3661 atccatcaaa agtggaccgt tccgcagcct acgcagcacg ttatgtcgcg aaaaacatcg
     3721 ttgctgctgg cctggccgat cgttgtgaaa ttcaggtttc ctacgcaatc ggcgtggctg
     3781 aaccgacctc catcatggta gaaactttcg gtactgagaa agtgccttct gaacaactga
     3841 ctctgctggt acgtgagttc ttcgacctgc gcccatacgg actgattcag atgctggatc
     3901 tgctgcaccc gatctacaaa gaaaccgcag catacggtca ctttggtcgt gaacatttcc
     3961 cgtgggaaaa aaccgacaaa gcgcagctgc tgcgcgatgc tgccggtctg aagtaatctt
     4021 tcttcacctg cgttcaaagg ccagcctcgc gctggccttt ttcttttacc tcaccgcggt
     4081 ggataggcgt tcacgccgca tccggcaaaa aaactgcccg cacaataaca tcattcttcc
     4141 tgatcacgtt tcacggcaaa ttaccatcac aactgaaacc gattacacca accacaccag
     4201 caaaatattt gtaatatttt catattatta ttcagctttc acagttgtta catttctttt
     4261 cagtaaagtc ttaattgcag atgacagcgt ttaatctatg atgatataac tcaattattt
     4321 tcatgccctt aaatcataac taagataaat gttagtgtaa gcgattacac tgatgtgatt
     4381 tgcttcacat ctttttacgt cgtactcacc tatcttaatt cacaataaaa aataactata
     4441 ttggagggca tcatgcctga cgctaaaaaa caggggcggt caaacaaggc aatgacgttt
     4501 ttcgtctgct tccttgccgc tctggcggga ttactctttg gcctggatat cggtgtaatt
     4561 gctggcgcac tgccgtttat tgcagatgaa ttccagataa cttcgcacac gcaagaatgg
     4621 gtcgtaagct ccatgatgtt cggtgcggca gtcggtgcgg tgggcatcgg ctggctctcc
     4681 tttaaactcg ggcgcaaaaa gagcctgatg atcggcgcaa tcctgtttgt tgccggttca
     4741 ctgttctctg cggctgcgcc aaacgttgaa gtactgattc tttcgcgcgt tctgctgggg
     4801 ctggcggtgg gtgtggcctc ttataccgca ccactgtacc tgtcagaaat tgcgccggaa
     4861 aaaattcgcg gcagtatgat ctcgatgtat cagttgatga tcactatcgg gatcctcggt
     4921 gcttatcttt ctgataccgc cttcagctac accggtgcat ggcgctggat gctgggtgtg
     4981 attatcatcc cggcaatttt gctgctgatt ggtgtcttct tcctgccaga cagcccacgt
     5041 tggtttgccg ccaaacgccg ttttgttgat gccgaacgcg tgctgctacg cctgcgtgac
     5101 accagcgcgg aagcgaaacg cgaactggat gaaatccgtg aaagtttgca ggttaaacag
     5161 agtggctggg cgctgtttaa agagaatagc aacttccgcc gcgcggtgtt ccttggcgta
     5221 ctgttacagg taatgcagca attcaccggg atgaacgtca tcatgtatta cgcgccgaaa
     5281 atcttcgaac tggcgggtta taccaacacc accgagcaaa tgtgggggac agtgattgtc
     5341 ggcctgacca acgtacttgc cacctttatc gcaatcggcc ttgttgaccg ctggggacgt
     5401 aaaccaacgc taacgctggg cttcctggtg atggctgctg gcatgggcgt actcggtaca
     5461 atgatgcata tcggtatcca ctctccgtcg gcgcagtatt tcgccatcgc catgctgctg
     5521 atgtttattg tcggttttgc catgagtgcc ggtccgctga tttgggtact gtgctccgaa
     5581 attcagccgc tgaaaggccg cgattttggc atcacctgct ccaccgccac caactggatt
     5641 gccaacatga tcgttggcgc aacgttcctg accatgctca acacgctggg taacgccaac
     5701 accttctggg tgtatgcggc tctgaacgta ctgtttatcc tgctgacatt gtggctggta
     5761 ccggaaacca aacacgtttc gctggaacat attgaacgta atctgatgaa aggtcgtaaa
     5821 ctgcgcgaaa tcggcgctca cgattaatct cctcaggctt cctcccgtcg cggaggaagc
     5881 cgcctcttgc agtcatcttt tcttcgctct atcctctgcc gctatgaaaa catcccgtct
     5941 ccctatcgcc atccaacagg ccgttatgcg ccgcctgcgg gaaaaactcg cccaggccaa
     6001 cctgaagcta gggcgtaact acccggagcc aaaactctct tacacccagc gcggaacctc
     6061 cgccggaaca gcctggctgg aaagctatga aatccgcctc aatcccgttt tgctgttgga
     6121 aaacagtgaa gcttttattg aagaagtggt accgcacgaa ctggcacatt tgctggtatg
     6181 gaaacatttc ggccgcgtag cgccacatgg caaagagtgg aagtggatga tggaaagcgt
     6241 gctgggcgtt cccgcccgtc gtacgcatca gttcgaactg caatccgtgc gtcgcaacac
     6301 cttcccctac cgctgtaagt gccaggaaca tcagcttacc gtacgccgcc ataatcgcgt
     6361 agttcgtggc gaggccatct atcgctgtgt tcactgcggt gaacagctgg ttgcgaaata
     6421 accacctgaa ctatcaggaa ctttcctgat ctggctgatt gcataccaaa acaactttcg
     6481 ctacgttgct ggctcgtttt aacacggagt aagtgatgta ccgttatttg tctattgctg
     6541 cggtggtact gagcgcagca ttttccggcc cgacgttggc cgaaggtatc aatagttttt
     6601 ctcaggcgaa agccgcggcg gtaaaagtcc acgctgatgc gcccggtacg ttttattgcg
     6661 gatgtaaaat taactggcag ggcaaaaaag gcgttgttga tctgcaatcg tgcggctatc
     6721 aggtgcgcaa aaatgaaaac cgcgccagcc gcgtagagtg ggaacacgtc gttcccgcct
     6781 ggcaattcgg tcaccagcgc cagtgctggc aggacggtgg acgtaaaaac tgcgctaaag
     6841 atccggtcta tcgcaagatg gaaagcgata tgcataacct gcagccgtca gtcggcgagg
     6901 tgaatggcga tcgcggcaat tttatgtaca gccagtggaa tggcggcgaa ggccagtacg
     6961 gtcaatgcgc catgaaggtc gatttcaaag aaaaagctgc cgaaccacca gcgcgtgcac
     7021 gcggtgccat tgcgcgcacc tacttctata tgcgcgacca atacaacctg acactctctc
     7081 gccagcaaac gcagctgttc aacgcatgga acaagatgta tccggttacc gactgggagt
     7141 gcgagcgcga tgaacgcatc gcgaaggtgc agggcaatca taacccgtat gtgcaacgcg
     7201 cttgccaggc gcgaaagagc taacctacac tagcgggatt ctttttgtta acccctaccc
     7261 cacgcgtaca accgcgtggg gagacgacgc ggatttttaa ctatgcgtat cccccgcatt
     7321 tatcatcctg aaccactgac cagccattct cacatcgcgc tttgcgaaga tgccgccaac
     7381 catatcgggc gcgtactgcg catggggccg gggcaggcgt tgcaattgtt tgacggtagc
     7441 aaccaggtct ttgacgccga aattaccagc gccagcaaaa aaagcgtgga agtgaaggtg
     7501 ctggaaggcc agatcgacga tcgcgaatct ccgctgcata ttcacctcgg tcaggtgatg
     7561 tcgcgtggtg aaaaaatgga atttactatc cagaaatcga tcgaactcgg tgtaagcctc
     7621 attacgccac ttttttctga gcgctgcggc gttaaactgg atagtgaacg tctgaacaag
     7681 aagcttcagc agtggcagaa gattgcaatt gctgcctgtg agcagtgtgg tcgtaaccgg
     7741 gtgccggaaa tccgtccagc gatggatctg gaagcctggt gtgcagagca ggatgaagga
     7801 ctgaaactga atcttcaccc gcgcgccagt aacagcatca atacgttgcc tttaccggtt
     7861 gaacgcgtcc gcctgctgat tggcccggaa ggcggtttat cggcagatga aattgccatg
     7921 actgcccgct atcaatttac tgatatcctg ttgggacctc gcgttttgcg tacagagaca
     7981 actgcgctca ccgccattac cgcgctacaa gtacgatttg gcgatttggg ctaacggaga
     8041 agaataatga tcaagctcgg catcgtgatg gaccccatcg caaacatcaa catcaagaaa
     8101 gattccagtt tcgctatgtt gctggaagca cagcgtcgtg gttacgaact tcactatatg
     8161 gagatgggcg atctgtatct gatcaatggc gaagcccgcg cccatacccg cacgctgaac
     8221 gtgaagcaga actacgaaga gtggttttcg ttcgtcggtg aacaggatct gccgctggcc
     8281 gatctcgatg tgatcctgat gcgtaaagac ccgccgtttg ataccgagtt tatctacgcg
     8341 acctatattc tggaacgtgc cgaagagaaa gggacgctga tcgttaacaa gccgcagagc
     8401 ctgcgcgact gtaacgagaa actgtttacc gcctggttct ctgacttaac gccagaaacg
     8461 ctggttacgc gcaataaagc gcagctaaaa gcgttctggg agaaacacag cgacatcatt
     8521 cttaagccgc tggacggtat gggcggcgcg tcgattttcc gcgtgaaaga aggcgatcca
     8581 aacctcggcg tgattgccga aaccctgact gagcatggca cttgctactg catggcgcaa
     8641 aattacctgc cagccattaa agatggcgac aaacgcgtgc tggtggtgga tggcgagccg
     8701 gtaccgtact gcctggcgcg tattccgcag gggggcgaaa cccgtggcaa tctggctgcc
     8761 ggtggtcgcg gtgaacctcg tccgctgacg gaaagtgact ggaaaatcgc ccgtcagatc
     8821 gggccgacgc tgaaagaaaa agggctgatt tttgttggtc tggatatcat cggcgaccgt
     8881 ctgactgaaa ttaacgtcac cagcccaacc tgtattcgtg agattgaagc agagtttccg
     8941 gtgtcgatca ccggaatgtt aatggatgcc atcgaagcac gtttacagca gcagtaaccc
     9001 accttagcga gaaggatctc gttgagactc tgagtgacag cgcccttctt tccacgcata
     9061 ctgggcgctg ttgctttttt gaaccaggaa acagaacctc tgacaatgaa tttacagcat
     9121 cactttctta ttgccatgcc tgctctccag gatccgattt tccgtcgttc cgtggtctac
     9181 atttgcgaac ataataccaa tggtgcaatg gggatcatcg tcaacaagcc gctggaaaat
     9241 ctcaaaattg aagggattct ggaaaagctg aagatcacgc cggagccgcg tgatgaatca
     9301 atccgtctgg ataaaccggt tatgctcggc ggcccactgg ctgaagatcg cgggtttatt
     9361 ttgcatactc cgccctccaa ttttgcttcc agcattcgca tttcagacaa cacggtgatg
     9421 accacttccc gcgatgtgct ggaaacgctc ggcaccgata aacaaccgtc tgacgtattg
     9481 gtggctctgg gctatgcctc ctgggaaaaa ggtcaactgg aacaagaaat tctcgataac
     9541 gcgtggctaa cggccccggc ggatctgaat attctgttca aaacgccgat tgccgaccgc
     9601 tggcgcgagg cggcaaaact gattggtgtg gatattctca ccatgcctgg tgtggcagga
     9661 cacgcctgat gagtggaacc ttactcgcct tcgacttcgg caccaaaagc attggcgtag
     9721 cggtcggcca acgcattacc ggcaccgctc gccctttgcc tgcaattaaa gcgcaggacg
     9781 gtacgccgga ctggaacctc atcgaacgtt tactgaaaga gtggcagcct gacgaaatca
     9841 tcgtcggttt gccgctgaat atggacggca ccgaacagcc gttaaccgcc agagcgcgca
     9901 aatttgccaa ccgtattcat ggtcgtttcg gtgttgaagt aaagctccat gacgagcgtc
     9961 ttagcactgt ggaagcccgt tccggtcttt ttgaacaggg cggctatcgg gcgctcaaca
    10021 aaggcaaagt tgactctgcc tctgcggtta ttattctcga aagctatttc gagcagggat
    10081 attaaggcga tctaaaagcc tggcggatag tcgttaccgc catcttgccg gatgcggcgt
    10141 gaacgcctta tccagcacac atctggcagc ggctgtaggt ctgataagac gcgttagcgt
    10201 cgcatcagac atttattgcc tttataggcc ctataaactt gcgcatcggg catggcaacg
    10261 tcacaaacgc ccttccccca cccgctgctg ataactctgc tgaaacgtta acatccccac
    10321 cttctgcccg gtttgaataa catgcggtaa ctggtgggtt ttcccttcgc gaatcaaatt
    10381 ccccaccgcg ggagtgttaa tcagtaattc aaacagcgcc acgcgtcctt cctgtttatc
    10441 cacttccagc ttttgtgaca gcactgcccg taaactaccc gcaagttgat tacgtacggg
    10501 atctttttcc tgcgccggaa atgaatccac cagtcgctca actgcctgcg cggcaccacg
    10561 tgtatgtaag gttgccagca ccagatgccc ggtttctgcc gccgtcagcg ccagacggat
    10621 tgtctcactg tcacgtagct ctccgagcaa aatcacatca ggatcttccc gcaatgcagc
    10681 ccgcaacccc gatgcgaacg tcatacagtg caaaccaatt tcccgctgct ggatcaaaca
    10741 tcgctggctg gcatagagat attccacagg atcttccagc gtcagaatat gcgcatcggc
    10801 atgttgatta agatagccaa ccatcgccgc cagcgtggta gatttgccac tccccgtagc
    10861 ccccgtcacc agaatcaggc cattctcgct cttgagtaat tccggcaata ccggtggtgc
    10921 accaagctgt tcgagctgtg gacagtgcga aggtaacaac cgtaatgcca gagaaatacc
    10981 ttgccgttgc gcgaacgcac tgccacgcaa ccgctggttt tccgccagcg acacggcaaa
    11041 atccagctgg ccattttcca gcaatattgc ccgctgatcg tcatccagcc actcccgcag
    11101 tagctcttcg acgtccggcg catcaaacgg cgcagcttcc attctgccac gaatgcgcca
    11161 tcgtgcgggc caggcgctgc acaggtgtag atccgagacg ttatgcttta cactaagggc
    11221 cacaatttct tccatattca tactaagatc ctcggaaaat gaacgatatt gcgcataacc
    11281 tggcacaggt ccgggacaaa atctcagcgg ctgcaacgcg ttgcggccgt tctccagaag
    11341 aaattacgct gcttgcagtc agtaaaacaa aacctgcgag cgccatcgca gaagccattg
    11401 atgccgggca gcgtcaattt ggtgaaaact acgttcagga aggggtagat aaaattcgcc
    11461 actttcagga actgggcgta acaggattag aatggcattt tattggcccg ttgcagtcta
    11521 ataaaagccg cctagtggca gagcatttcg actggtgtca taccatcgac cgtttgcgca
    11581 ttgctacccg cctcaacgag cagcgcccgg cagaacttcc ccctcttaac gtactgattc
    11641 aaattaatat tagtgatgaa aacagtaagt ccgggattca actggcagaa ctggacgagc
    11701 tggcagcggc ggtcgctgaa ctaccgcgtt tacgtctgcg cgggctgatg gcaatccctg
    11761 cgcttgagtc agaatatgta aggcagtttg aagttgcacg ccaaatggct gtagcatttg
    11821 ccggactgaa aacgcgctac ccgcatatcg acacgctctc tctgggaatg tcggacgata
    11881 tggaagccgc cattgcggca ggtagcacga tggttcgtat cggcactgca atttttggtg
    11941 cgcgtgatta ctctaaaaaa taaggaatta aaggaacgcc atgaatacgt tgactttcct
    12001 gctttcaacg gtcattgagc tgtatacaat ggtgctgtta ttacgcatct ggatgcagtg
    12061 tgctcattgt gatttttaca cccccttctc acagtttgta gtgaaggtag cgcagccaat
    12121 tatcgggcca ctgcgccgcg ttattccggc aatgggtccc attgacagcg cctcgctgct
    12181 ggttgcctat attctcagtt ttatcaaagc cattgtgctg tttgaagtgg tgaccttcct
    12241 gccaatcatc tggattgccg gtttactgat tctgctgaaa accatcggct tgctgatttt
    12301 ctgggtcctg ctggtgatgg cgattatgag ctgggtaagc cagggtcgta gcccgattga
    12361 atacgtgctg attcagctgg ccgatccgct gctgcgcccg attcgccgct tgctaccggc
    12421 aatgggtggg attgatttct cgccgatgat cctcgttctg ctgctgtatg ccatcaatat
    12481 gggtgtcgca gaagtattac aggcaaccgg aaacatgctg ctgccggggc tgtggatggc
    12541 gttatgagtg ccgtaacagt taatgatgac ggtctggttt tacggctcta tattcagccg
    12601 aaagccagcc gtgattctat tgtcggttta catggcgacg aagttaaagt cgccattacc
    12661 gcgccgccgg ttgacggcca ggccaacagt catctggtga agtttctcgg taagcaattc
    12721 cgggttgcca aaagccaggt ggtgattgaa aaaggcgaac ttggccgcca caaacaaatt
    12781 aaaatcatta atccgcaaca aatcccgcca gaaatcgcgg cgttaattaa ttaggtatcc
    12841 tatgcaaaaa gttgtcctcg caaccggcaa tgccggtaaa gtgcgtgagc tggcgtcgct
    12901 gcttagcgac tttggtcttg atatcgtggc ccaaacagac ctcggcgttg attccgctga
    12961 agaaaccggc ctaaccttta tcgaaaacgc gattctgaaa gcgcgccatg cggcaaaagt
    13021 gaccggttta ccggcaattg ccgacgactc tggtctggcg gtagatgcgc tgggtggcgc
    13081 gccgggtatt tactccgcgc gttattccgg tgaagacgcg accgatcaaa agaatctgca
    13141 aaaactgttg gaaacactga aagacgtacc ggacgaccaa cgtcaggcgc gtttccactg
    13201 cgtgctggta tatctgcgtc acgcggaaga tccgactccg ctggtatgcc acggcagctg
    13261 gccgggcgtg attactcgcg aaccagcggg cactggtggc tttggttatg atccaatctt
    13321 cttcgtacct tccgaaggga aaaccgccgc cgaactgacc cgcgaagaaa agagcgccat
    13381 ttcccaccgt ggtcaggcgt tgaaactgct gctggacgct ttacgtaatg gttaaattac
    13441 cgccgctgag tctctacatt cacatcccgt ggtgcgtgca gaaatgcccg tactgcgatt
    13501 tcaactctca cgcgttgaaa ggagaagtgc cgcacgacga ttatgttcag catctgcttt
    13561 gcgatctgga caatgatgtg gcttacgctc agggccgtga agtaaagaca atctttattg
    13621 gcggtggtac gccgagcctg ctttccggcc cggcgatgca aacgctgcta gacggcgtgc
    13681 gtgcgcgttt gccgctggca gcggatgcag aaattactat ggaagcgaac ccaggcacgg
    13741 tagaagcgga tcgctttgtc gattatcagc gtgctggtgt aaaccgcatc tctattggtg
    13801 tgcagagttt tagcgaagaa aagctgaaac gacttgggcg tattcatggc ccgcaagaag
    13861 cgaaacgagc ggcaaagctg gcgagcggtt tagggttacg tagctttaac cttgatttga
    13921 tgcatggact accggatcaa tcactggaag aggcgcttgg cgatctacgc caggccattg
    13981 aactgaatcc gccgcatctt tcctggtatc aactgactat cgaacctaat acgctgtttg
    14041 gctcgcgacc gccggtgctg ccggacgatg acgcgctgtg ggatattttc gaacaggggc
    14101 atcagttatt aaccgcagcg ggttatcagc aatatgaaac ttccgcttac gccaaacccg
    14161 gttatcagtg ccagcacaat ctcaactact ggcgctttgg tgactacatc ggtattggct
    14221 gcggcgcgca cggcaaagtg accttcccgg atgggcgcat tctgcgtacc actaaaacgc
    14281 gtcatcctcg tggttttatg caaggaaggt atctggaaag ccagcgtgat gtcgaagccg
    14341 cagataagcc gtttgagttc tttatgaatc gcttccgtct gctggaggcc gcgccgcgcg
    14401 tggagtttat tgcgtatacc gggctttgcg aagatgtgat tcgcccacag ttagacgagg
    14461 cgattgccca gggttatctc accgaatgtg cggattactg gcagataacg gaacatggta
    14521 aattattctt aaactcactt cttgaattat tcctcgctga gtaatcatta atgcgcctgc
    14581 gggcgcatta atcacttctt ttatcccccc gttaattaac ctcggcagaa atatatttct
    14641 tactgaataa atatcgacga catttattat atatatttag tgaatattta ctaatcattc
    14701 attgattata aatatgtaca acctggcgca caaatttaat atttaatttc tgactttatt
    14761 tcaagttgtg ataaacatag acaagttgtc agacaggtta accaatttgt cgcacaaatc
    14821 actgataagc acaattagat atcaaccaat tcccatcctt caacagggag tttttttatg
    14881 aaaatgaggt catttacccg ctcattagtc tgcgcatcct tgctggctct ggttagcact
    14941 ggcgtaaacg ccgcagaaaa agtggcgtta aagttagcac acaacctgga aagaagtcat
    15001 gttgtacatc agagttttga agagctggcg aaagaggtaa aacagctgtc aaaaggtaat
    15061 atggtgatcc gtatttatcc cagcagccag atgggtaatg cgcgagaaac tatggaattg
    15121 ttgcaaaatg gcgcactgga tatgaccaaa ggttccgcca gtgatctgga gtcttttgat
    15181 aatatttatg ctatctataa tttgccgttc ttattaagtt gtttttggtg aagttggtaa
    15241 agaaattatg gactcaacaa aagacaaagg cttttttgct ttatcagctt atgtagcggg
    15301 tactcgcagt ttctatgcga aaaaaccgat tacaaagcca gaagatctta aaggactgaa
    15361 aattcgtgtt cagccaagtc cgaccaccat caaaatgatt gaactgatgg gtggttcacc
    15421 cactccaatt tcatttggcg aagtttatac tgcgatgcag caaggtgtag tagatggcgc
    15481 agaaaataac gtgccttcct ggatgcagac tcgacatatt gaaattgcca aagttttttc
    15541 tgaagatgaa cacgcctcaa ttcctgactt tttggttatc tccaccaaaa catggaataa
    15601 attaacccca gaacagcaac aaatcctcga aacagcggcg aagaaatccg aagcttatca
    15661 acaaaaattg tgggaaaaaa ttgacgctga cacacgtgct caggcgaaag ctatgggggg
    15721 tgaaattgta aaagtggata aagcgccatt ccgtaaagcc gtgcaacctc tgtttgacga
    15781 cttcaaaaaa gatccaaaac aagctgcttt gttagagaaa ttcgacaacg ctgctcaata
    15841 atattccggg ccagcctctg gccctgattt tccaggatgc ttacaatgat atttaaccgc
    15901 ctgaaacttg cggtagaccg cgtcattgcc acgttttctg tcgccgtaat gcttgctctg
    15961 gttgtatgcg tagtctggca agtatttagc cgctacgtac ttaaccagcc aagtacatta
    16021 accgatgagc tggcacgctt tttgatgatc tgggtaggat tattaggtgc tgcatatacc
    16081 gtcggcgcgc agcgacatct ctctattgat ttatttgctc tcgccctgaa taagcgcaaa
    16141 caattacttc ttagtatagt cattaacgtt ctcattttag gttttgccgg ttcggtcatt
    16201 gtgactggtg gcctgaaatt aattgataaa acactggcaa catcacaggt atctgccgct
    16261 atgcaaatac cgatgggata cgtatatatc attctgccat taagtggagt ggtaatgatg
    16321 ttttatgcct tgtgttttat taatcaaagc attcaacaac tgaaacaacc tgcgcaggag
    16381 gccagttaat gga
//
LOCUS       DAETQL010000085        16096 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.085, whole genome shotgun sequence.
ACCESSION   DAETQL010000085 DAETQL010000000
VERSION     DAETQL010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16096
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.085"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..588
                     /gene="tuf"
                     /locus_tag="K0W65_003109"
     CDS             <1..588
                     /gene="tuf"
                     /locus_tag="K0W65_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBI3096272.1"
                     /translation="IPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEV
                     EIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKP
                     HTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKM
                     VVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            771..965
                     /gene="bfd"
                     /locus_tag="K0W65_003110"
     CDS             771..965
                     /gene="bfd"
                     /locus_tag="K0W65_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HBI3096273.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            1038..1514
                     /gene="bfr"
                     /locus_tag="K0W65_003111"
     CDS             1038..1514
                     /gene="bfr"
                     /locus_tag="K0W65_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HBI3096274.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1511..1978)
                     /locus_tag="K0W65_003112"
                     /pseudo
     CDS             complement(1511..1978)
                     /locus_tag="K0W65_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            2357..2668
                     /gene="rpsJ"
                     /locus_tag="K0W65_003113"
     CDS             2357..2668
                     /gene="rpsJ"
                     /locus_tag="K0W65_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBI3096275.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2701..3330
                     /gene="rplC"
                     /locus_tag="K0W65_003114"
     CDS             2701..3330
                     /gene="rplC"
                     /locus_tag="K0W65_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBI3096276.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3341..3946
                     /gene="rplD"
                     /locus_tag="K0W65_003115"
     CDS             3341..3946
                     /gene="rplD"
                     /locus_tag="K0W65_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBI3096277.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3943..4245
                     /gene="rplW"
                     /locus_tag="K0W65_003116"
     CDS             3943..4245
                     /gene="rplW"
                     /locus_tag="K0W65_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBI3096278.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            4263..5084
                     /gene="rplB"
                     /locus_tag="K0W65_003117"
     CDS             4263..5084
                     /gene="rplB"
                     /locus_tag="K0W65_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBI3096279.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            5101..5379
                     /gene="rpsS"
                     /locus_tag="K0W65_003118"
     CDS             5101..5379
                     /gene="rpsS"
                     /locus_tag="K0W65_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBI3096280.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5394..5726
                     /gene="rplV"
                     /locus_tag="K0W65_003119"
     CDS             5394..5726
                     /gene="rplV"
                     /locus_tag="K0W65_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBI3096281.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5744..6445
                     /gene="rpsC"
                     /locus_tag="K0W65_003120"
     CDS             5744..6445
                     /gene="rpsC"
                     /locus_tag="K0W65_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBI3096282.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6458..6868
                     /gene="rplP"
                     /locus_tag="K0W65_003121"
     CDS             6458..6868
                     /gene="rplP"
                     /locus_tag="K0W65_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBI3096283.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6868..7059
                     /gene="rpmC"
                     /locus_tag="K0W65_003122"
     CDS             6868..7059
                     /gene="rpmC"
                     /locus_tag="K0W65_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBI3096284.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            7059..7313
                     /gene="rpsQ"
                     /locus_tag="K0W65_003123"
     CDS             7059..7313
                     /gene="rpsQ"
                     /locus_tag="K0W65_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBI3096285.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7478..7849
                     /gene="rplN"
                     /locus_tag="K0W65_003124"
     CDS             7478..7849
                     /gene="rplN"
                     /locus_tag="K0W65_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBI3096286.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7860..8174
                     /gene="rplX"
                     /locus_tag="K0W65_003125"
     CDS             7860..8174
                     /gene="rplX"
                     /locus_tag="K0W65_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBI3096287.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            8189..8728
                     /gene="rplE"
                     /locus_tag="K0W65_003126"
     CDS             8189..8728
                     /gene="rplE"
                     /locus_tag="K0W65_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBI3096288.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8743..9048
                     /gene="rpsN"
                     /locus_tag="K0W65_003127"
     CDS             8743..9048
                     /gene="rpsN"
                     /locus_tag="K0W65_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBI3096289.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            9082..9474
                     /gene="rpsH"
                     /locus_tag="K0W65_003128"
     CDS             9082..9474
                     /gene="rpsH"
                     /locus_tag="K0W65_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBI3096290.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9487..10020
                     /gene="rplF"
                     /locus_tag="K0W65_003129"
     CDS             9487..10020
                     /gene="rplF"
                     /locus_tag="K0W65_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBI3096291.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            10030..10383
                     /gene="rplR"
                     /locus_tag="K0W65_003130"
     CDS             10030..10383
                     /gene="rplR"
                     /locus_tag="K0W65_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBI3096292.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10398..10901
                     /gene="rpsE"
                     /locus_tag="K0W65_003131"
     CDS             10398..10901
                     /gene="rpsE"
                     /locus_tag="K0W65_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBI3096293.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10905..11084
                     /gene="rpmD"
                     /locus_tag="K0W65_003132"
     CDS             10905..11084
                     /gene="rpmD"
                     /locus_tag="K0W65_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBI3096294.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            11088..11522
                     /gene="rplO"
                     /locus_tag="K0W65_003133"
     CDS             11088..11522
                     /gene="rplO"
                     /locus_tag="K0W65_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBI3096295.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11530..12861
                     /gene="secY"
                     /locus_tag="K0W65_003134"
     CDS             11530..12861
                     /gene="secY"
                     /locus_tag="K0W65_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBI3096296.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12893..13009
                     /gene="rpmJ"
                     /locus_tag="K0W65_003135"
     CDS             12893..13009
                     /gene="rpmJ"
                     /locus_tag="K0W65_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBI3096297.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            13156..13512
                     /gene="rpsM"
                     /locus_tag="K0W65_003136"
     CDS             13156..13512
                     /gene="rpsM"
                     /locus_tag="K0W65_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBI3096298.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13529..13918
                     /gene="rpsK"
                     /locus_tag="K0W65_003137"
     CDS             13529..13918
                     /gene="rpsK"
                     /locus_tag="K0W65_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBI3096299.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13952..14572
                     /gene="rpsD"
                     /locus_tag="K0W65_003138"
     CDS             13952..14572
                     /gene="rpsD"
                     /locus_tag="K0W65_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBI3096300.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14598..15587
                     /gene="rpoA"
                     /locus_tag="K0W65_003139"
     CDS             14598..15587
                     /gene="rpoA"
                     /locus_tag="K0W65_003139"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBI3096301.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15628..16011
                     /gene="rplQ"
                     /locus_tag="K0W65_003140"
     CDS             15628..16011
                     /gene="rplQ"
                     /locus_tag="K0W65_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBI3096302.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4141 a         3784 c         4292 g         3879 t
ORIGIN      
        1 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
       61 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      121 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      181 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      241 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      301 aagccgcaca ccaagttcga atctgaagtg tacattctgt ccaaagatga aggcggccgt
      361 catactccgt tcttcaaagg ctaccgtccg cagttctact tccgtactac tgacgtgact
      421 ggtaccatcg aactgccgga aggcgtagag atggtaatgc cgggcgacaa catcaaaatg
      481 gttgttaccc tgatccaccc gatcgcgatg gacgacggtc tgcgtttcgc aatccgtgaa
      541 ggcggccgta ccgttggcgc gggcgttgtt gctaaagttc tgggctaatt acacgttaat
      601 tagttttgaa ttgaaaaggg cgcttcggcg ccctttttgc tgcccgtaat ttgagctcga
      661 aatatttagt cgtaattttg ctcgctgaac tatggcgcaa ataaaactgt gctattgtaa
      721 tcataaccat tctcatttac actttgtgca gaatttgaac gggagcaatc atgtacgttt
      781 gtttgtgtaa tggtgtaagt gacaaaaaaa ttcgccaggc agtccgtcag tttcatccgc
      841 aatctttcca acaattgcgt aagtttattc ctgtgggaaa tcaatgcggt aagtgcattc
      901 gcgccgcgcg cgaagtgatg caggacgagt tgatgcagat gccagaattt aaagagattg
      961 cctgaagccc acgcttattt tttgacatcc tgctagcccc atctacgctc taatagtgga
     1021 agcggaggga ctataaaatg aaaggtgatg ttaaaatcat aaattatctc aataaactat
     1081 tgggaaatga gcttgtcgca atcaatcagt attttctcca tgcccggatg tttaaaaact
     1141 ggggcctgat gcgtctcaat gacgtcgaat accatgaatc catcgatgag atgaaacacg
     1201 ctgataaata tatcgagcgt attttgtttt tagaaggaat ccccaactta caggatttag
     1261 gcaaactggg gattggtgaa gatgtcgagg agatgctgca atctgacctg cgattagaac
     1321 tggaaggcgc gaaggatctg cgtgaggcta tcgcatatgc cgacagcgtt catgactatg
     1381 ttagccgcga tatgatgatc gagatccttg ctgatgaaga aggtcatatc gactggctcg
     1441 aaactgaact ggatctcatc ggtaaaatcg gcctacaaaa ttacctgcaa tcacaaatta
     1501 aagttaaaga ttaacggcct atcagcaaac tatcccagcc tataacgaaa cccgcagccg
     1561 ccagaaatgg cccaaaaggc agcgggtttt ttaatgattc ttttcctctc atgagcagac
     1621 caataactac ggctccgcaa gcgaatgagg cagcaaggaa aaccagtcgt ggtaagaagg
     1681 cccatgaatg ctaggcgcca agggcggcga gaaacttcac atcaccatag cctaatcctt
     1741 ctttatgtcg cagtatgcga tacccccagt aaatgacagc gaatgtgccg taaccaacaa
     1801 ttgcccccca taacgcatcg gctaaaccat caggatgaca aacttgatag aacaaaagac
     1861 ctgaccagag taacgggcac gtaaatctgt caggtaacaa gccatgcttt gcatcccaga
     1921 aaaaaagtag ggctgataaa caagcgtaca aaattagaaa gggaagggta gtgtccataa
     1981 aacatcctct gtaattggag tatcaccatc gaataactcc ggatgctcag caacagagag
     2041 agcgctgatt tgttaacctg attccggtga aaaacgctat cttcacaacc ttacaaattt
     2101 cgtggaatgc agctcgcaat ctgcgcgttg cgattaattt ctacccgatg cttgcgtcag
     2161 gctcagattt gcgtataatg cgcgggcttg tcgtagttga cagcaggttc aatctgaacc
     2221 tcagttagcc gaatttggct acctaacaat gctcccaatc ggggagctac gtaagaacgg
     2281 ttacactctc ccatcaatcg taatgggtct gaggagtaat cattttcgtt tataaaataa
     2341 ttggagctct ggtctcatgc agaaccaaag aatccgtatc cgcctgaaag cgtttgatca
     2401 tcgtctgatc gatcaagcaa ccgcggaaat cgtcgagact gccaagcgca ctggtgcgca
     2461 ggtccgtggt ccgatcccgc tgccgacacg caaagagcgc ttcactgttc tgatctcccc
     2521 gcacgtcaac aaagacgcgc gcgatcagta cgaaatccgt actcacttgc gtctggttga
     2581 catcgttgag ccaaccgaga aaaccgttga tgctctgatg cgtctggatc tggctgccgg
     2641 tgtagacgtg cagatcagcc tgggttaatc aggtcattga gcgattgaga ggttgaaaca
     2701 atgattggtt tagtcggtaa aaaagtgggt atgacccgta tcttcacaga agacggcgtt
     2761 tctatcccag taaccgtaat cgaagttgaa gcaaaccgcg ttactcaggt taaagacctg
     2821 gctaacgatg gctaccgtgc tattcaggtg accaccggtg ctaaaaaagc taaccgtgtg
     2881 accaagcctg aagctggcca cttcgctaaa gctggcgtag aagctggccg tggtctgtgg
     2941 gaattccgcc tggctgaagg cgaagagttc actgtaggtc agagcattag cgttgaactg
     3001 tttgctgacg ttaaaaaagt tgacgtaact ggcacctcta aaggtaaagg tttcgcaggt
     3061 accgttaagc gctggaactt ccgtacccag gacgctactc acggtaactc cttgtctcac
     3121 cgcgttccgg gttctatcgg tcagaaccag actccgggca aagtgttcaa aggcaagaaa
     3181 atggcaggtc agatgggtaa cgaacgtgta accgttcaga gccttgacgt agtacgcgtt
     3241 gacgctgagc gcaacctgct gctggttaaa ggtgctgtcc cgggtgcaac cggtagcgac
     3301 ctgatcgtta aaccagctgt gaaggcgtaa ggagatagca atggaattag tattgaaaga
     3361 cgcgcagagc gcgctgactg tttccgaaac taccttcggt cgtgatttca acgaagcgct
     3421 ggttcaccag gttgttgttg cttatgcagc tggtgctcgt cagggtactc gtgctcagaa
     3481 gactcgtgct gaagtaactg gttccggtaa aaaaccgtgg cgccagaaag gcaccggccg
     3541 tgcgcgttct ggttctatca agagcccgat ctggcgttct ggtggcgtga cctttgctgc
     3601 tcgtccgcag gaccacagtc aaaaagttaa caagaagatg taccgcggcg cgctgaaaag
     3661 catcctgtcc gaactggtac gtcaggatcg tctgatcgtt gtcgagaagt tctctgtaga
     3721 agcgccgaaa actaagctgc tggcacagaa actgaaagac atggctctgg aagatgtgct
     3781 gatcatcacc ggtgagctgg acgaaaacct gttcctggct gcgcgcaacc tgcagaaggt
     3841 tgacgtacgc gatgcaactg gtatcgaccc ggttagcctg atcgccttcg acaaagtcgt
     3901 aatgactgct gatgctgtta agcaagttga ggagatgctg gcatgattcg tgaagaacgt
     3961 ctgctgaagg tgctgcgtgc accgcacgtt tctgaaaaag cgtctactgc gatggaaaaa
     4021 tccaacacca tcgtactcaa agttgctaaa gacgcgacca aagcagaaat caaagctgct
     4081 gtgcagaaac tgtttgaagt cgaagtcgaa gtcgttaaca ccctggtagt taaagggaaa
     4141 gttaaacgtc acggacagcg tatcggtcgt cgtagcgact ggaaaaaagc ttacgtcacc
     4201 ctgaaagaag gccagaatct ggacttcgtt ggcggcgctg agtaagtcgg aggagtaata
     4261 caatggcagt tgttaaatgt aaaccgacat ctccgggtcg tcgccacgta gttaaagtgg
     4321 ttaaccctga gctgcacaag ggcaaacctt ttgctccgtt gctggaaaaa aacagcaaat
     4381 ccggtggtcg taacaacaat ggccgtatca ccactcgtca tatcggtggt ggccacaagc
     4441 aggcttaccg tattgttgac ttcaaacgca acaaagacgg tatcccggca gttgttgaac
     4501 gtcttgagta cgatccgaac cgttccgcga acatcgcgct ggttctgtac aaagacggtg
     4561 aacgccgtta catcctggcc cctaaaggcc tgaaagctgg cgaccagatt cagtctggcg
     4621 ttgatgctgc aatcaaacca ggtaacaccc tgccgatgcg caacatcccg gttggttcta
     4681 ctgttcataa cgtagaaatg aaaccaggta aaggcggtca gctggcacgt tccgctggta
     4741 cttacgttca gatcgttgct cgtgatggtg cttatgtcac cctgcgtctg cgttctggtg
     4801 aaatgcgtaa agtagaagca gactgccgtg caactctggg cgaagttggc aatgctgagc
     4861 atatgctgcg cgttctgggt aaagcaggtg ctgcacgctg gcgtggtgtt cgtccgaccg
     4921 ttcgcggtac cgcgatgaac ccggtagacc acccacatgg tggtggtgaa ggtcgtaact
     4981 ttggtaagca cccggtaact ccgtggggcg ttcagaccaa aggtaagaag acccgcagca
     5041 acaagcgtac tgataaattc atcgtacgtc gccgtagcaa ataattttag aggataagcc
     5101 atgccacgtt ctctcaagaa aggtcctttt attgacctgc acttgctgaa gaaggtagag
     5161 aaagcggtgg aaagcggaga caagaagccc ctgcgcactt ggtcccgtcg ttcaacgatc
     5221 tttcctaaca tgatcggttt gaccatcgct gtccataatg gtcgtcagca cgttccggta
     5281 tttgtaaccg acgaaatggt cggtcacaaa ctgggtgaat tcgcaccgac tcgtacttat
     5341 cgcggccacg ctgctgataa aaaagcgaag aagaaataag gtaggaggaa gagatggaaa
     5401 ctattgctaa acatcgccat gctcgttctt ctgctcagaa ggttcgcctt gttgctgacc
     5461 tgattcgcgg taagaaagtg tcgcaggctc tggatatttt gacctacacc aacaagaaag
     5521 cggctgtaca ggtcaagaaa gttctggaat ctgccattgc taacgctgaa cacaacgatg
     5581 gcgctgacat tgacgatctg aaagttacga aaattttcgt agacgaaggc ccgagcatga
     5641 agcgcattat gccgcgtgca aaaggtcgtg cagatcgcat cctgaagcgc accagccaca
     5701 tcactgtggt tgtgtccgat cgctgagact ctggagacta gcaatgggtc agaaagtaca
     5761 tcctaatggt attcgcttgg gtattgtaaa accatggaac tctacttggt ttgcgaacac
     5821 caaagaattc gctgacaacc tggacagcga ttttaaagta cgtcagtacc tgactaagga
     5881 actggctaaa gcgtccgtat ctcgtatcgt tatcgagcgt ccggctaaga gcatccgtgt
     5941 aaccattcac actgctcgcc cgggtatcgt tatcggtaaa aaaggtgaag acgtagaaaa
     6001 actgcgtaag gtcgtagcgg acatcgctgg cgttcctgca cagatcaaca tcgccgaagt
     6061 tcgtaagcct gaactggacg caaaactggt tgctgacagc atcacttctc agctggaacg
     6121 tcgcgttatg ttccgtcgtg ctatgaagcg tgctgtacag aacgcaatgc gtctgggcgc
     6181 taaaggtatt aaagttgaag ttagcggccg tctgggcggc gcggaaatcg cacgtaccga
     6241 atggtaccgc gaaggtcgcg taccgctgca cactctgcgt gctgacatcg actacaacac
     6301 ctctgaagcg cacaccactt acggtgtaat cggcgttaaa gtgtggatct tcaaaggcga
     6361 gatcctgggt ggtatggctg ctgttgaaca accggaaaaa ccggctgctc agcctaaaaa
     6421 gcagcagcgt aaaggccgta aataaggagc gtcgctgatg ttacaaccaa agcgtacaaa
     6481 attccgtaaa atgcacaaag gccgtaaccg cggtctggcg cagggtacgg atgttagctt
     6541 cggcagcttc ggtctgaaag ctgttggccg tggtcgtctg actgcccgtc agatcgaagc
     6601 agcacgtcgt gctatgaccc gtgcagttaa gcgtcaaggt aagatctgga tccgtgtgtt
     6661 cccggacaaa ccgatcactg aaaagccgct ggcagtgcgt atgggtaaag gtaaaggtaa
     6721 cgtggagtat tgggttgcct tgattcagcc gggtaaagtc ctgtatgaaa tggacggtgt
     6781 tccggaagag ctggcccgtg aagcattcaa gctggcagca gcgaaactgc cgattaaaac
     6841 cacctttgta actaagacgg tgatgtaatg aaagcaaaag agctgcgtga gaagagcatt
     6901 gaagagctga acaccgagct gctgaacctg ctgcgtgagc agttcaacct gcgtatgcag
     6961 gctgcaagtg gccagctgca acagtctcac ctgttgaagc aagtgcgtcg cgatgtcgca
     7021 cgcgttaaga ctttactgaa cgagaaggcg ggtgcgtaat gaccgataaa atccgtactc
     7081 tgcaaggtcg cgttgttagc gacaaaatgg agaaatccat tgttgttgct atcgaacgtt
     7141 ttgtgaaaca cccgatctac ggaaaattca tcaagcgtac gaccaaactg cacgtacatg
     7201 acgagaacaa cgaatgcggt atcggtgacg tggttgaaat ccgcgaatgc cgtccgctgt
     7261 ccaagactaa atcctggacg ctggttcgcg ttgtagagaa agcggttctg taatacagta
     7321 cactctctcg atacgaataa acggctcaga aatgagccgt ttattttttc tacccatatc
     7381 cttgaagcgg tgttataatg ccgcgccctc gatatgggga tttttaacga cctgattttc
     7441 gggtctcagt agtagttgac attagcggag cactaaaatg atccaagaac agactatgct
     7501 gaacgtcgcc gacaactccg gtgcacgtcg cgtaatgtgt atcaaggttc tgggtggctc
     7561 gcaccgtcgc tacgcaggcg taggcgacat catcaagatc accatcaaag aagcaattcc
     7621 gcgtggtaag gtcaaaaaag gtgatgtgct gaaggcggta gtggtgcgca ccaagaaggg
     7681 tgttcgtcgc ccggacggtt ctgtcattcg cttcgatggt aatgcttgtg ttcttctgaa
     7741 caacaacagc gagcagccta tcggtacgcg tatttttggg ccggtaactc gtgagcttcg
     7801 tagtgagaag ttcatgaaaa ttatctctct ggcaccagaa gtactctaag gagcgaatca
     7861 tggcagcgaa aatccgtcgt gatgacgaag ttatcgtgtt aaccggtaaa gataaaggta
     7921 aacgcggtaa agttaagaat gtcctgtctt ccggcaaggt cattattgaa ggtatcaacc
     7981 tggttaagaa acatcagaag ccggttccgg ccctgaacca accgggtggc atcgttgaaa
     8041 aagaagccgc tattcaggtt tccaacgtag caatcttcaa tgcggcaacc ggcaaggctg
     8101 accgtgtagg ctttagattc gaagacggca aaaaagtccg tttcttcaag tctaacagcg
     8161 aaactatcaa gtaatttgga gtagtacgat ggcgaaactg catgattact acaaagacga
     8221 agtagttaaa aaactcatga ctgagtttaa ctacaattct gtcatgcaag tccctcgggt
     8281 cgagaagatc accctgaaca tgggtgttgg tgaagcgatc gctgacaaaa aactgctgga
     8341 taacgcagca gcagacctgg cagcaatctc cggtcaaaaa ccgctgatca ccaaagcacg
     8401 caaatctgtt gcaggcttca aaatccgtca gggctatccg atcggctgta aagtaactct
     8461 gcgtggcgaa cgcatgtggg agttctttga gcgcctgatc actattgctg tacctcgtat
     8521 ccgtgacttc cgtggcctgt ccgctaagtc tttcgacggt cgtggtaact acagcatggg
     8581 tgtccgtgag cagatcatct tcccagaaat cgactacgat aaagtcgacc gcgttcgtgg
     8641 tttggatatt accattacca ctactgcgaa atctgacgaa gaaggccgcg ctctgctggc
     8701 tgcctttgac ttcccgttcc gcaagtaagg tagggttact aaatggctaa gcaatcaatg
     8761 aaagcacgcg aagtaaaacg cgtagcttta gctgataaat acttcgcgaa acgcgctgaa
     8821 ctgaaagcga tcatctctga tgtgaacgct tccgacgaag atcgttggaa cgctgttctc
     8881 aagctgcaga ctctgccgcg tgattccagc ccgtctcgtc agcgtaaccg ctgccgtcaa
     8941 acaggtcgtc cgcatggttt cctgcggaag ttcgggttga gccgtattaa ggtccgtgaa
     9001 gccgctatgc gcggtgaaat cccgggtctg aaaaaggcta gctggtaatt gtcaccaatt
     9061 gaatcacggg agtaaagaca gatgagcatg caagatccga tcgcggatat gctgacccgt
     9121 atccgtaacg gtcaggccgc gaacaaagct gcggtcacca tgccttcctc caagctgaaa
     9181 gtggcaatcg ccaacgtgct gaaggaagaa ggttttattg aagattttaa agttgaaggc
     9241 gacaccaagc ctgaactgga acttactctg aagtatttcc agggcaaagc tgttgtagaa
     9301 agcattcagc gtgtcagccg cccaggtctg cgcatctata aacgtaaaga tgagctgccg
     9361 aaagttatgg cgggtctggg tatcgcagtt gtttctacct ctaaaggtgt tatgactgat
     9421 cgtgcagcgc gccaggctgg tcttggtggc gaaattatct gctacgtagc ctaatcggag
     9481 gaaaaaatgt ctcgtgttgc taaagcaccg gtcgttgttc ctgccggcgt tgacgtaaaa
     9541 atcaacggtc aggttattac gatcaaaggt aaaaacggcg agctgactcg tactctcaac
     9601 gatgctgttg aagttaaaca tgcagataat accctgacct tcggtccgcg tgatggttac
     9661 gcagacggtt gggcacaggc tggtaccgcg cgtgccctgc tgaactcaat ggttatcggt
     9721 gttaccgaag gcttcactaa gaagctgcag ctggttggtg taggttaccg tgcagcggtt
     9781 aaaggcaatg tgattaacct gtctctgggt ttctctcatc ctgttgacca tcagctgcct
     9841 gcgggtatca ctgctgaatg tccgactcag actgaaatcg tgctgaaagg cgctgataag
     9901 caggtgatcg gccaggttgc agcggatctg cgcgcctacc gtcgtcctga gccttacaaa
     9961 ggcaaggggg ttcgttacgc cgacgaagtc gtgcgtacca aagaggctaa gaagaagtaa
    10021 ggtaacacta tggataagaa atctgctcgt atccgtcgtg cgacccgcgc acgccgcaag
    10081 ctccaggagc tgggcgcaac tcgcctggtg gtacatcgta ccccgcgtca catttacgca
    10141 caggtaattg caccgaacgg ttctgaagtt ctggtagctg cttctactgt agaaaaagct
    10201 atcgctgaac aactgaagta caccggtaac aaagacgcgg ctgcagctgt gggtaaagct
    10261 gtcgctgaac gcgctctgga aaaaggcatc aaagatgtat cctttgaccg ttccgggttc
    10321 caatatcatg gtcgtgtcca ggcactggca gatgctgccc gtgaagctgg ccttcagttc
    10381 taaggtagag gtgtaagatg gctcacatcg aaaaacaagc tggcgaactg caggaaaagc
    10441 tgatcgcggt aaaccgcgta tctaaaaccg ttaaaggtgg tcgtattttc tccttcacag
    10501 ctctgactgt agttggcgat ggtaacggtc gcgttggttt tggttacggt aaagcgcgtg
    10561 aagttccagc agcgatccag aaagcgatgg aaaaagcccg tcgcaatatg attaacgtcg
    10621 cgctgaataa cggcactcta caacaccctg ttaaaggtgt tcacacgggt tctcgcgtat
    10681 tcatgcagcc ggcttccgaa ggtaccggta tcatcgccgg tggtgcaatg cgcgccgttc
    10741 tggaagtcgc tggggttcat aacgttctgg ctaaagcata tggttccacc aacccgatca
    10801 acgtggttcg tgcaactatt gatggcctgg aaaatatgaa ttctccagaa atggtcgctg
    10861 ccaagcgtgg taaatccgtt gaagaaattc tggggaaata aaccatggca aagactatta
    10921 aaattactca aacccgcagt gcaatcggtc gtctgccgaa acacaaggca acgctgcttg
    10981 gcttgggtct gcgtcgtatt ggtcacaccg tagagcgcga ggatactcct gctattcgcg
    11041 gtatgatcaa cgcggtttcc ttcatggtta aagttgagga gtaagagatg cgtttaaata
    11101 ctctgtctcc ggccgaaggc tccaaaaagg cgggtaaacg cctgggtcgt ggtatcggtt
    11161 ctggcctcgg taaaaccggt ggtcgtggtc acaaaggtca gaagtctcgt tctggcggtg
    11221 gcgtacgtcg cggtttcgag ggtggtcaga tgcctctgta ccgtcgtctg ccgaaattcg
    11281 gcttcacttc tcgtaaagca gcgattacag ccgaagttcg tctatctgac ctggctaaag
    11341 tagaaggcgg tgttgtagac ctgaacacgc tgaaagcggc taacattatc ggtatccaga
    11401 tcgagttcgc gaaagtgatc ctggctggcg aagtaacgac tccggtaact gttcgtggcc
    11461 tgcgtgttac taaaggcgct cgtgctgcta tcgaagctgc tggcggtaaa atcgaggaat
    11521 aagtagcaga tggctaaaca accgggatta gattttcaaa gtgccaaagg tggcttaggc
    11581 gagctgaaac gcagactgct gtttgttatc ggtgcgctga ttgtgttccg tattggctct
    11641 tttattccga tccctggtat tgatgccgct gtacttgcca aactgcttga gcaacagcga
    11701 ggcaccatca ttgagatgtt taacatgttc tctggtggtg ctctcagccg tgcttctatc
    11761 tttgctctgg ggatcatgcc gtatatttcg gcgtcgatca ttatccagct gctgacggtg
    11821 gttcacccaa cgttggcaga aattaagaaa gaaggggagt ctggtcgtcg taagatcagc
    11881 cagtacaccc gttacggtac tctggtgctg gcaatattcc agtcgatcgg tattgctacc
    11941 ggtctgccga atatgcctgg tatgcaaggc ctggtgatta acccgggctt tgcattctac
    12001 ttcaccgctg ttgtaagtct ggtcacagga acgatgttcc tgatgtggtt gggcgaacag
    12061 atcactgaac gaggtatcgg caacggtatt tcaatcatta tcttcgccgg tattgtcgcg
    12121 ggactcccgc cagccattgc ccatactatc gagcaagcgc gtcaaggcga cctgcacttc
    12181 ctcgtgttgc tgttggttgc agtattagta tttgcagtga cgttctttgt tgtgtttgtt
    12241 gagcgtggtc aacgccgcat tgtggtaaac tacgcgaaac gtcagcaagg tcgtcgtgtc
    12301 tatgctgcac agagcacaca tttaccgctg aaagtgaata tggcgggggt aatcccggca
    12361 atcttcgctt ccagtattat tctgttcccg gcgaccatcg cgtcatggtt cgggggcggt
    12421 actggttgga actggctgac aacaatttcg ctgtatttgc agcctgggca accgctttat
    12481 gtgttactct atgcgtctgc aatcatcttc ttctgtttct tctacacggc gttggttttc
    12541 aacccgcgtg aaacagcaga taacctgaag aagtccggtg catttgtacc aggaattcgt
    12601 ccgggagagc aaacggcgaa gtatatcgat aaagtaatga cccgcctgac cttggttggt
    12661 gcgctgtata ttacctttat ctgcctgatc ccggagttca tgcgtgatgc aatgaaagta
    12721 ccgttctact tcggtgggac ctcactgctt atcgttgttg tcgtgattat ggactttatg
    12781 gctcaagtgc aaactctgat gatgtccagt cagtatgagt ctgcattgaa gaaggcgaac
    12841 ctgaaaggct acggccgata attggtcgcc cgagaagtta cggagagtaa aaatgaaagt
    12901 tcgtgcttcc gtcaagaaat tatgccgtaa ctgcaaaatc gttaagcgtg atggtgtcat
    12961 ccgtgtgatt tgcagtgccg agccgaagca taaacagcgc caaggctgat tttttcgcat
    13021 atttttcttg caaagttggg ttgagctggc tagattagcc agccaatctt ttgtatgtct
    13081 gtacgtttcc atttgagtat cctgaaaacg ggcttttcag catggaacgt acatattaaa
    13141 tagtaggagt gcatagtggc ccgtatagca ggcattaaca ttcctgatca taagcatgcc
    13201 gtaatcgcat taacttcgat ttatggcgtc ggcaagaccc gttctaaagc catcctggct
    13261 gcagcgggta tcgctgaaga tgttaagatc agtgagctgt ctgaaggaca aatcgacacg
    13321 ctgcgtgacg aagttgccaa atttgtcgtt gaaggtgatc tgcgccgtga aatcagcatg
    13381 agcatcaagc gcctgatgga tcttggttgc tatcgcggtt tgcgtcatcg tcgtggtctc
    13441 ccggttcgcg gtcagcgtac caagaccaac gcacgtaccc gtaagggtcc gcgcaaaccg
    13501 atcaagaaat aatcggggtg attgaataat ggcaaaggca ccaattcgtg cacgtaaacg
    13561 tgtaagaaaa caagtctctg acggcgtggc tcatatccat gcttctttca acaacaccat
    13621 cgtgactatc actgatcgtc agggtaacgc gttgggttgg gcaacagccg gtggttccgg
    13681 tttccgtggt tctcgcaaat ccactccgtt tgcagctcag gttgcagcag agcgttgcgc
    13741 tgacgccgtg aaagaatacg gcatcaagaa tctggaagtt atggttaaag gtccgggtcc
    13801 aggccgcgaa tctactattc gtgctctgaa cgccgcaggt ttccgcatca ctaacattac
    13861 tgatgtgact ccgatccctc ataacggttg tcgtccgccg aaaaaacgtc gcgtataacg
    13921 cctcgttttc caggtttgtt ggagaaagaa aatggcaaga tatttgggtc ctaagctcaa
    13981 gctgagccgt cgtgagggca ccgacttatt ccttaagtct ggcgttcgcg cgatcgatac
    14041 caagtgtaaa attgaacaag ctcctggcca gcacggtgcg cgtaaaccgc gtctgtctga
    14101 ctatggtgtg cagttgcgtg aaaagcaaaa agttcgccgt atctatggtg tgctggagcg
    14161 tcagttccgt aactactaca aagaagcagc acgtctgaaa ggcaacaccg gtgaaaacct
    14221 gttggctctg ctggaaggtc gtctggacaa cgttgtatac cgtatgggct tcggtgccac
    14281 tcgtgcagaa gcacgtcagc tggttagcca taaagcaatt atggtaaacg gtcgtgttgt
    14341 taacatcgct tcttatcagg ttagtccgaa tgacgttgta agcattcgtg agaaagcgaa
    14401 gaagcagtct cgcgtgaaag ccgctctgga gctggctgag cagcgtgaaa agccaacttg
    14461 gctggaagtt gatgctggca agatggaagg tacgtttaag cgtaagccgg agcgttctga
    14521 tctgtctgcg gacattaacg aacacctgat cgtcgagctt tactccaagt aaagcttagt
    14581 accaaagaga ggacacaatg cagggttctg tgacagagtt tctaaaaccg cgcctggttg
    14641 atatcgagca agtgagttcg acgcacgcca aggtgaccct tgagccttta gagcgtggct
    14701 ttggccatac tctgggtaac gcactgcgcc gtattctgct ctcatcgatg ccgggttgcg
    14761 cggtgaccga ggttgagatt gatggtgtac tacatgagta cagcaccaaa gaaggcgttc
    14821 aggaagatat cctggaaatc ctgctcaacc tgaaagggct ggcggtgaga gttcagggca
    14881 aagatgaagt tattcttacc ttgaataaat ctggcattgg ccctgtgact gcagccgata
    14941 tcacccacga cggtgatgtt gaaatcgtca agccgcagca cgtgatctgc cacctgaccg
    15001 atgagaacgc gtctattagc atgcgtatca aagttcagcg cggtcgtggt tatgtgccgg
    15061 cttctacccg aattcattcg gaagaagatg agcgcccaat cggccgtctg ctggtcgacg
    15121 catgctacag ccctgtggag cgtattgcct acaatgttga agcagcgcgt gtagaacagc
    15181 gtaccgacct ggacaagctg gtcatcgaaa tggaaaccaa cggcacaatc gatcctgaag
    15241 aggcgattcg tcgtgcggca accattctgg ctgaacaact ggaagctttc gttgacttac
    15301 gtgatgtacg tcagcctgaa gtgaaagaag agaaaccaga gttcgatccg atcctgctgc
    15361 gccctgttga cgatctggaa ttgactgtcc gctctgctaa ctgccttaaa gcagaagcta
    15421 tccactatat cggtgatctg gtacagcgta ccgaggttga gctccttaaa acgcctaacc
    15481 ttggtaaaaa atctcttact gagattaaag acgtgctggc ttcccgtgga ctgtctctgg
    15541 gcatgcgctt ggaaaactgg ccaccggcaa gcatcgctga cgagtaaccg gatcacaggt
    15601 taaggtttta ctgagaagga taaggtcatg cgccatcgta agagtggtcg tcaactgaac
    15661 cgcaacagca gccatcgcca ggctatgttc cgcaatatgg caggttcact ggttcgtcat
    15721 gaaatcatca agacgactct gcctaaagcg aaagagctgc gccgcgtagt tgagccgctg
    15781 attactcttg ccaagactga tagcgttgct aatcgtcgtc tggcattcgc ccgtactcgt
    15841 gataacgaga tcgtggcaaa actgtttaac gaactgggcc cgcgtttcgc gagccgtgcc
    15901 ggtggttaca ctcgtattct gaagtgtggc ttccgtgcag gcgacaacgc gccgatggct
    15961 tacatcgagc tggttgatcg ttcagagaaa gcagaagctg ctgcagagta atctgaagca
    16021 atgtaaaaaa acccgccccg gcgggttttt ttatacccgt agtatcccca cttatctaca
    16081 atagctgtac tctttt
//
LOCUS       DAETQL010000086        15933 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.086, whole genome shotgun sequence.
ACCESSION   DAETQL010000086 DAETQL010000000
VERSION     DAETQL010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.086"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            449..1507
                     /gene="citC"
                     /locus_tag="K0W65_003141"
     CDS             449..1507
                     /gene="citC"
                     /locus_tag="K0W65_003141"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBI3096303.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1522..1818
                     /gene="citD"
                     /locus_tag="K0W65_003142"
     CDS             1522..1818
                     /gene="citD"
                     /locus_tag="K0W65_003142"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBI3096304.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1815..2723
                     /gene="citE"
                     /locus_tag="K0W65_003143"
     CDS             1815..2723
                     /gene="citE"
                     /locus_tag="K0W65_003143"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBI3096305.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2734..4266
                     /gene="citF"
                     /locus_tag="K0W65_003144"
     CDS             2734..4266
                     /gene="citF"
                     /locus_tag="K0W65_003144"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBI3096306.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4270..4820
                     /gene="citX"
                     /locus_tag="K0W65_003145"
                     /pseudo
     CDS             4270..4820
                     /gene="citX"
                     /locus_tag="K0W65_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4795..5673
                     /gene="citG"
                     /locus_tag="K0W65_003146"
     CDS             4795..5673
                     /gene="citG"
                     /locus_tag="K0W65_003146"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBI3096307.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5724..7186
                     /gene="citT"
                     /locus_tag="K0W65_003147"
                     /pseudo
     CDS             5724..7186
                     /gene="citT"
                     /locus_tag="K0W65_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7300..8106
                     /gene="rna"
                     /locus_tag="K0W65_003148"
     CDS             7300..8106
                     /gene="rna"
                     /locus_tag="K0W65_003148"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBI3096308.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8335..8745
                     /gene="rnk"
                     /locus_tag="K0W65_003149"
     CDS             8335..8745
                     /gene="rnk"
                     /locus_tag="K0W65_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBI3096309.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8976..10214)
                     /locus_tag="K0W65_003150"
     CDS             complement(8976..10214)
                     /locus_tag="K0W65_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HBI3096310.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10435..10863
                     /gene="uspG"
                     /locus_tag="K0W65_003151"
     CDS             10435..10863
                     /gene="uspG"
                     /locus_tag="K0W65_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBI3096311.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10984..12549)
                     /gene="ahpF"
                     /locus_tag="K0W65_003152"
     CDS             complement(10984..12549)
                     /gene="ahpF"
                     /locus_tag="K0W65_003152"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBI3096312.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12794..13357)
                     /gene="ahpC"
                     /locus_tag="K0W65_003153"
     CDS             complement(12794..13357)
                     /gene="ahpC"
                     /locus_tag="K0W65_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBI3096313.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13729..14475
                     /gene="dsbG"
                     /locus_tag="K0W65_003154"
     CDS             13729..14475
                     /gene="dsbG"
                     /locus_tag="K0W65_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBI3096314.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14685..15587
                     /locus_tag="K0W65_003155"
     CDS             14685..15587
                     /locus_tag="K0W65_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3096315.1"
                     /translation="MANLYDLKKLDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15734..15933
                     /locus_tag="K0W65_003156"
                     /pseudo
     CDS             15734..15933
                     /locus_tag="K0W65_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3956 a         4119 c         3855 g         4003 t
ORIGIN      
        1 aaagatacgc agcggaaatg ccagtctttg gaaaaatgca aactgtttat tctcgttaag
       61 ctgcaacatt gttttccttg cgggttgtcc cgagcctctt tgacggatgt aattaaaatg
      121 ttgaatcaaa atacatagat attaattatg atcgtggcaa attttattca ttaaatcaat
      181 taaatcactt aaacctgcga gtcatttcgt aattacatta attaaaaaac ttaaaaccat
      241 taaataaata aaaaaccact aactctatgt gaaataaatc aaaatttcac gccgaaatac
      301 tccttaggat gtatagcgaa aggagaaaaa gatatacctc gatcaccccc ctttctccca
      361 agtgaaaata aaaggttatc agtttgcaac attgaacaac attcgttgca aatcgataac
      421 aacatgcacc ttcaggatac tatttattat gttcggcaat gatattttca cccgcgtaaa
      481 acgttcagaa aataaaaaaa tggcggaaat cgcccaattc ctgcatgaaa atgatttgag
      541 cgttgacacc acagtcgaag tatttattac cgtaacccgc gatgaaaagc ttatcgcgtg
      601 cggtggaatt gccggaaata ttattaaatg cgttgctatc agtgaatccg ttcgcggtga
      661 aggactggcg ctgacattag ccactgaact gataaacctc gcctatgagc ggcacagcac
      721 gcatctgttt atttatacca aaaccgaata cgaggcgctg ttccgccagt gcggtttttc
      781 cacgctgacc tgcgtacccg gcgtgatggt gctgatggaa aacagcgcca cgcgactgaa
      841 acgctatgcc gaatcgctga aaaaatttcg tcatccaggg aacaagattg gctgcattgt
      901 gatgaacgcc aatcccttta cgaatggtca ccgttatctg attcaacaag ctgcggcaca
      961 gtgcgactgg ttgcatctgt ttttagtcaa agaagattct tcacgcttcc cctatgaaga
     1021 ccggctggat ctggtgttaa aaggcaccgc cgatattcca cgcctgactg tgcatcgcgg
     1081 ctcagaatac atcatctccc gcgctacgtt cccttgctac ttcattaaag aacagagcgt
     1141 cattaaccat tgttacaccg aaattgatct gaagattttc cgtcagtacc tcgctcccgc
     1201 gcttggtgta actcaccgct ttgtcggtac tgaacccttt tgtcgcgtta ccgcccagta
     1261 caaccaggat atgcgctact ggctggaaac gccgactatc tccgcagcgc ccatcgaact
     1321 ggttgaaatt gagcggctgc gttaccagga gatgccgata tccgcttccc gggtacgtca
     1381 actgctggcg aaaaacgatc tcacggctat cgcgccgctg gtccctgcag tcacgctgca
     1441 ttatttgcag aacctgcttg agcactcccg ccaggacgcg gcagctcgtc aaaagacccc
     1501 cgcatgagaa acaggtgaaa aatgaaaata aaccagcccg ccgttgcagg cacccttgag
     1561 tctggggatg tgatgatacg catcgcccca ctcgatacgc aggatatcga cctgcaaatc
     1621 aatagcagcg ttgagaaaca gtttggcgat gcaattcgca ccaccattct ggacgttctc
     1681 gcccgctaca acgtgcgcgg cgtacagctg aatgtcgatg acaaaggcgc actggactgc
     1741 attttacatg cacgactgga agccctgctg gcacgcgcca gcggtatccc ggctctgcca
     1801 tgggaggatt gccaatgatt tccgcttcgc tgcaacaacg taaaactcgc acccgccgca
     1861 gcatgttgtt tgtgcctggt gccaatgccg cgatggtcag caactccttc atctacccgg
     1921 ctgatgccct gatgtttgac ctcgaagact ccgtggcatt acgtgaaaaa gacaccgccc
     1981 gccgcatggt ttatcacgcg ctgcaacatc cgctgtatcg cgatattgaa accattgtgc
     2041 gtgtcaacgc gctggattcc gaatggggtg ttaacgacct ggaagccgtc gttcgcggtg
     2101 gtgcggacgt tgtgcgtctg ccgaaaaccg ataccgctca ggatgttctg gatattgaaa
     2161 aagagatcct gcgtatagaa aaagcctgtg gtcgtgaacc cggcagcacc ggcctgctgg
     2221 cggcgattga atctccgctg ggcattaccc gcgcagtgga aatcgctcac gcttccgagc
     2281 gtttgatcgg tatcgccctc ggtgcagaag actatgtgcg caacctgcgt acagaacgct
     2341 ccccggaagg aactgaactg ctgttcgcac gctgttccat tttgcaggcc gcgcgctctg
     2401 cgggtattca ggcgttcgat accgtctatt ccgacgctaa caacgaagcc ggattcctgc
     2461 aagaagccgc ccacatcaaa cagctgggct ttgacggcaa atcgctgatc aacccgcgtc
     2521 agattgatct gctgcacaac ctctacgcac cgacccagaa agaagtggat cacgcccacc
     2581 gcgtcgtaga agccgctgaa gccgccgctc gcgaaggcct cggcgtggtt tccctgaacg
     2641 gcaagatggt ggacggtccg gttatcgatc gcgcccgtct ggtgctctcc cgtgcagaac
     2701 tttccggcat ccgcgaagaa taaggcaatc aaaatgacgc agaaaattga acaatctcaa
     2761 cgacaagaac gggtagcggc ctggaatcgt cgcactgaat gcgatcttgc cgctttccag
     2821 aactcaccaa agcaaaccta ccaggctgaa aaagcgcgcg atcgcaaact gtgcgccaac
     2881 ctggaagaag cgattcgtcg ctcaggttta caggacggca tgacggtttc cttccatcac
     2941 gctttccgtg gcggtgacct gaccgtcaat atggtgatgg acgtcatcgc gaagatgggc
     3001 tttaaaaacc tgaccctggc gtccagctcc ctgagtgatt gccatgcgcc gctggtagaa
     3061 cacattcgcc agggcgtggt tacccgcatt tatacctctg gcctacgcgg tccactggcg
     3121 gaagagatct cccgtggtct gctggcggaa ccggtgcaga tccactctca cggcggtcgt
     3181 gtgcatctgg tacagagcgg cgaactgaat atcgacgtgg ctttcctcgg cgtcccgtcc
     3241 tgtgatgaat tcggtaatgc caacggctac accggtaaag cctgctgcgg ctccctcggc
     3301 tatgcaatgg ttgatgccga caacgcaaaa caggtcgtga tgcttaccga agaactgctg
     3361 ccttatccgc ataatccggc aagcattgag caagatcagg ttgatttgat cgtcaaagtt
     3421 aatcgcgttg gcgatgctgc aaaaatcggc gctggtacta cccgtatgac cactaacccg
     3481 cgcgaactgc ttattgcccg tagcgctgcg gatgtgattg tcaactctgg ctacttcaaa
     3541 gaaggtttct ccatgcaaac cggcaccggc ggcgcttcgc tggcggtaac ccgtttcctg
     3601 gaagacaaaa tgcgtagccg cgatattcgc gccgacttcg ccctcggcgg tattaccgcg
     3661 acgatggttg atctgcacga aaaaggtctg atccgcaaac tgctggatgt gcagagcttt
     3721 gacagccatg ctgcgcaatc gctggcccgt aaccccaatc acatcgaaat cagcgccaac
     3781 cagtacgcta actggggttc gaaaggcgca tcggttgatc gtctcgacgt ggtggtactg
     3841 agcgcgctgg aaattgacag gcagttcaac gttaacgtgc tgaccggttc tgacggcgta
     3901 ctgcgtggcg cttccggtgg tcactgcgat accgcgattg cctctgcgct ttccatcatc
     3961 gtcgcgccac tggtacgcgg tcgtattccg actctggtgg ataacgtgct gacctgcatc
     4021 accccgggct ccagtgtcga tattctggtc acagaccacg gtatcgcagt taacccggca
     4081 cgtccggaac tagcagaacg tctgcaggaa gcgggcatta aagtggtttc cattgagtgg
     4141 ctgcgcgaac gtgcgcgtct gctgaccggt gaaccacagc cgattgaatt caccgaccgc
     4201 gtcgttgccg ttgtgcgtta ccgcgatggc tcggtgatcg atgttgtgca tcaggtgaag
     4261 gaataagcca tgcacctgct tcctgaactc gccagccacc atgcggtgtc aattcccgag
     4321 ctgctcgtca gccgggatga aaggcaagca cggcaacacg cctggctcaa gcaccatcct
     4381 gttccactgg tctcctttac cgtggttgcg cctgggccga ttaaagacag cgaggtcaca
     4441 cgccgaattt ttaatcatcg cgtgacagcc ttgcgtgcct tagccacaaa gcagggctgg
     4501 caaattcagg agcaggctgc actggtttcc gccagcgggc cggagggcat gttgagcatt
     4561 gccgccccgg ctcgcgacct caagctcgcc accattgagc ttgaacatag tcatcctctc
     4621 gggcggttat gggatatcga tgtcctgacg cccgaaggcg aaattctctc ccgccgcgac
     4681 tattcactgc gcctcgccgc tgcctgttgt gcgagcaaag cgcagccgtc tgcgcgcgtg
     4741 gaaaaaccca tcaactgacc gatttactca accgcatgga ggcactgctg aacgatgtcg
     4801 atgcctgcaa cgtcaactaa aaccacaaag cttgcgacgt cattaatcga tgagtacgcc
     4861 ctgctgggct ggcgcgccat gctgactgaa gtcaatctgt caccgaaacc aggcctcgtg
     4921 gatcgcatta actgcggtgc gcacaaagat atggcgctgg aagatttcca ccgcagcgcg
     4981 ctggcgattc agggctggtt accccgtttc attgaatttg gtgcctgtag tgcagaaatg
     5041 gcaccggaag cggtactcca cggattacgc ccaattggta tggcttgcga aggtgatatg
     5101 ttccgcgcca ctgcgggcgt aaacacgcat aaaggcagca ttttttcttt agggctgcta
     5161 tgtgcggcaa ttggccgttt gcttcagctc aaccagccgg ttacgccaac aaccgtttgt
     5221 tctacggcgg caagtttctg ccgtggcctg accgatcgcg aactgcgtac caataattca
     5281 cgactgacgg caggtcaacg gttgtaccag cagcttggcc ttaccggcgc acgcggtgaa
     5341 gccgaagcag gttatccact ggtgatcaac cacgccttgc cgcattacct cactctgctg
     5401 gatcaggggt tagatcctga actggcattg ctcgatacct tgctcctact gatggctacc
     5461 aacggcgata ccaacgttgc atcgcgcggt ggcgaggggg gcctgcgctg gctacagcgc
     5521 gaggcgcaaa cattattgca aaaagggggc attcgaaccc ccaccgatct cgattatctc
     5581 cggcagttcg acagggagtg tatcgaacga aatctcagtc caggcggcag tgccgacctg
     5641 ctgatcctta cctgtttttt agcacagatt taattattta agcacttgat aaatttggaa
     5701 atattaattt tcggagaacc cgtatgtctt tagcaaaaga taatatatgg aaactattgg
     5761 ccccactggt ggtgatgggt gtcatgtttc ttatccctgt ccccgacggt atgccgccgc
     5821 aggcatggca ttacttcgct gtgtttgtgg caatgattgt cggcatgatc ctcgagccaa
     5881 ttccggcaac agcgatcagt tttattgcgg ttactatttg cgttattggc agtaattacc
     5941 tgctctttga tgccaaagaa ttagctgacc cagcgtttaa tgcgcaaaaa caggcgctga
     6001 aatggggcct ggctggtttt tccagcacca cggtatggct ggtatttggc gcatttattt
     6061 ttgcattagg gtatgaagtt tccgggttag gtcgtcgcat tgcccttttc ctggtgaaat
     6121 tcatggacaa acgcacgctg acgttgggtt atgcgattgt cattatcgac attctgctgg
     6181 caccgtttac accgtccaac accgcgcgta ccgggggtac ggtttttccg gtcattaaaa
     6241 acctgccgcc gttgtttaaa tcattcccga acgatccgtc cgcgcgtcgt attggcggct
     6301 atttgatgtg gatgatggtc attagtacca gtctgagttc gtccatgttt gtcaccggtg
     6361 cggaccaaac gtgctgggtc tggagttcgt cagcaaaatt gccggtatcc agattagctg
     6421 gttgcagtgg ttcctctgct tcctgccggt tggggttatc ttgcttatca ttgcgccgtg
     6481 gctttcctac gtgctgtaca aaccggaaat cacacacagt gaagaagtgg caacctgggc
     6541 gggtgatgaa ctaaaaacca tgggtgcgct gacacgcaga gagtggacgc tgattggcct
     6601 tgtattgctc agcttaggtt tgtgggtatt tggcagtgaa gtcattaatg ctactgcggt
     6661 tggtctgctg gcagtttcgc taatgctggc tctgcacgtt gtgccgtgga aagacattac
     6721 ccgctataac agcgcatgga acacgctggt caacctggca actctggttg tgatggctaa
     6781 cggcctgact cgttctggtt ttattgactg gttcgccggt accatgagta cgcacctgga
     6841 aggattctca ccaaacgcaa cggtgattgt actggttctg gtgttctact ttgcacacta
     6901 cctgtttgcc agcctgtctg cgcacaccgc aaccatgctg ccggttattc tggccgtcgg
     6961 taaaggtatt ccgggcgtac caatggaaca actgtgtatc ctgctggtgc tgtctatcgg
     7021 tatcatgggc tgtctgacgc cgtatgcaac cggtcctggg gtgattattt acggctgtgg
     7081 ctatgtgaaa tcaaaagatt actggcgtct tggcgcaatc ttcggggtga tttacatctc
     7141 tatgttgctg ttggttggct ggccgattct cgccatgtgg aactaatacg ttcgccaaac
     7201 gctgaataaa tcatcacgcc cgccaggtaa gcccacctgg cgggcttttt tatgatttaa
     7261 tagatagtat tcagtgtatg tatgagttcc acacccatta tgaaagcatt ctgccgtaac
     7321 gccgcgttgc tcgcggtttc tctgcttccc ttatcttctg ccaacgccgt agcgttgcag
     7381 gcaaaacagt atggcgattt tgatcgctat gtcctggccc tctcctggca aaccggattt
     7441 tgccagagtc aacacgatcg aaatcgtaac gaacgagatg aatgtcgcct gcaaaccgaa
     7501 acgaccaaca aaactgattt tctgaccgta catggtctgt ggccaggatt gcctaaatcg
     7561 gttgctgccc gtggtgttga tgaacgccgc tggatgcgct tcggttgcgc tactcgccca
     7621 atcccgaatc taccagaagc acgcgccagc cgaatgtgtt catcgccgga aaccgggtta
     7681 tcactggaaa cggccgctaa actaagtgaa gtcatgccag gagctggcgg acgttcctgc
     7741 ctggaacgct acgaatatgc caaacacggt gcctgctttg gttttgatcc ggacgcatac
     7801 ttcggtacga tggtacgcct gaatcaagaa attaaagaga gcgaagccgg aaaattcctt
     7861 gcggataatt acggtaaaac agtgagccgc cgtgactttg acgccgcctt tgccaaaagc
     7921 tggggaaaag agaacgtgaa agcagttaag ctaacgtgcc agggtaaccc tgcgtatttg
     7981 actgaaattc agatctcgat caaagctgac gccatcaacg ctccgctttc tgcaaactca
     8041 ttcttgccac aacctcaccc aggtaactgt ggcaaaacct ttgtgattga taaagcgggt
     8101 tattaatcct ctgatttaca aaacacgcta attcgtcttc cataaccatg ccgattaaga
     8161 attatacata atgcgctgca ttgaggaatt ccctgaatgt gacgcaaatc acttcaaaga
     8221 gtgagcaaca gtcagtatca tcatgtgagt taaaccctcg ccgcctgacg gtgagggttt
     8281 tcttttgggt atgtttatct gcgacactcg cagtaccgac gacatggagt aaaaatgtcc
     8341 agaccaacta tcatcattaa cgacctggat gccgaacgca tcgatattct gctggagcaa
     8401 cccgcctatg ctggtttgcc aatcgccgac gcgttaaacg cagagttgga tcgcgcccaa
     8461 atgtgttcgc cagaagagat gccacacgac gtggtgacaa tgaacagccg ggttaaattc
     8521 cgcaatctta gcgatggcga agtgcgtgtg cgcacgctgg tgtatccggc aaaaatgacc
     8581 gatagcaata ctcagctttc cgttatggct ccggtaggtg ccgcactgct ggggctgcgc
     8641 gttggcgatt ccattcactg ggaacttccg ggcggcgttg caacccacct tgaagtgctg
     8701 gaactcgaat accagccaga agctgctggc gactacctgc tttaatccca tctgcacaag
     8761 cgataactcg ctttgcccag aggatgcact cgcatcctct ttacgcaaaa tcagcaattt
     8821 ttcctggttc tcactgttca cttttgctgc aaaattattc cgtttcgttt gtgggaataa
     8881 ccatggcaga agcattttac atcctgatag gatttctgat tatggcggcg attatcgtca
     8941 tggctgtcct ttacctcgaa aaccactcct gacgatcata ttgttccccc cggcatcgca
     9001 ttcaccagac ctgaaaccgc cttctgcgcc gcctctgcgc tttgtgcacc gggtaccaga
     9061 atcaccttac ggcactcctc ttcacgtttt tcgaaaatct catatccccg ggcggcctct
     9121 tcaaacggca tatagtgggt aacaatttct tctggtttca gtaatccttt ctcaattaac
     9181 ggtaataatt ctcccagcca tgcgtgaacg tgggtctgtc ccattttaaa cgtcaaccct
     9241 ttatcaaagg cgtcgccaaa caggaaaccg tgaataaatc cagcgtagac gcccggtacg
     9301 ctaacaatgc cgccacgcct gaccgccgca atacactggc gcaacgcttt accgctgctg
     9361 ccctccagtt tcaggttagt cagcaccgtt tccgtggtgc tgcctttcgc ttcaaaaccg
     9421 acggcgtcta ttactgcatc cacgccccgg tgacctgccg tttgttcaat aattgactgt
     9481 gccggatcgc tatcttcatc gaaattaatc gggatcgcgc cgtagcggtc agcggcgaaa
     9541 cgcaagcggt agggatgatg atcaacaaca aaaatctgtt ccgcaccgag caaccgtgca
     9601 caggcgattg tcaacaatcc cacaggacca gcaccataga ctgcaacgct tgaaccttgt
     9661 tggatctgca cattttttgc tgcctgccat gccgttggca gaatatcaga aaggaaaagg
     9721 gctttatcat ctgaaagcaa aggcggtact ttaaacggcc ccacattccc tttcgggacg
     9781 cggacatatt ccgcctgccc gccaggaacg ccaccataca ggtgactata accaaacaat
     9841 gccgcgggag caggtatctg ttttttattg agcgcagcgc ctttacccgc attggtattt
     9901 tcgcaggcgg catattgctg taatcgacag aaaaaacagt cgccacaagc aatgacgaac
     9961 ggaattacca cccggtcgcc tttttgcaaa ttttttacgt ccttcccggt ttcaacgact
    10021 tcccccataa attcatgacc aaaaatatcg ccatgtttaa cctggggtat tttgcctcga
    10081 taaagatgga ggtcagagcc acagatcgcc gttaccgtaa tacgcagaat aatatcatct
    10141 gcctgttcaa tgcccggatc gggaacattt tctacctgaa cgtgatgtgg gccgtgataa
    10201 gtcaatgctt tcatgcaacc tccagagaca gttcaaaaaa gagtcagtct ttaagggtag
    10261 ctatcacagc gggaaaacgg gcggtaccgc aggttttggg agaattccta gtgttcagtg
    10321 agaatatatt aatcacagaa accgttcgta aggaaaaaga accagacaaa tatcagggat
    10381 aaatattata atcatggaga atgatttgtt catgattaac agggagaaag ggttatgtat
    10441 aaggcaatca ttatgccagt tgatgtattt gaaatggaat tgagcgacaa agctgttcgc
    10501 cacgctgaat tcctcgccca ggatgacgga gttattcatc tacttcacgt actacccggg
    10561 tcagccagcc tgagcctgca ccgttttgcc gctgatgtgc gtcgttttga agagcatctg
    10621 caacatgaag cagaagaacg tctgcaaacg atggtcagcc acttcaccat cgatccttcc
    10681 cgcattaaac aacatgtccg ttttggtagc gtgcgggatg aagtcaatga gttggcagaa
    10741 gaactggggg ctgatgttgt agttatcggt tctcgcaacc catcgatttc gacccatctg
    10801 ttaggttcta acgcctcgag cgtgatccgc cacgccaatc tgcctgtact ggtcgttcgc
    10861 taacagataa aagtatttat gccgccctgc ctggaacatc ccggcagggc tttttaatgc
    10921 cataaaaaag ccgccaggtt tgaccccggc ggctaagcaa ttgcaggtga atcttacttc
    10981 ttcttatgca gttttggtgc gaatcaggta gtcaaaagca ctcagagagg ctttggcacc
    11041 ttcgccagtg gcgatgatga tctgcttgta cggaaccgtc gtacagtcac ccgctgcgaa
    11101 cacgcctttg acgttggttt cacatttcgc atcaatgata atctcgccca tgcggttacg
    11161 ttcgactgcg ccttccagcc agttggtgtt cggcagcaga ccaatctgga caaaaatacc
    11221 ggccagttcg atgttgtgaa tattgccgct gacacgatct cgatattcca gaccaacgac
    11281 tttgctgccg tcgcctttca cttccgtggt ttgcgcattc agaataatgt cgacgttttt
    11341 caggctgcgc agtttgtcct gcagaacctg gtcggctttc atttctggcg caaattccag
    11401 cagcgttacg tgctcaacga tacccgccag gtcgattgcc gcttccacgc cggagttacc
    11461 gccgccgata accgctacgc gtttgccttt aaacagcggg ccgtcgcagt gcgggcagta
    11521 ggtcacgcct ttagtgcgat actgatcttc gcccggaacg ttcatgttgc gccattttgc
    11581 acccgtcgcc acgataatgc tgcgtgcttt cagtaccgcg ccagaagctg tttcaatctg
    11641 gtgtagacca ccttcaaccg ctgccgggat cagtttgctg gcgctctggc tgtcgatcac
    11701 atcaacgtcg tattcatcaa cgtgaacttt cagtgcgcct gccaacttct gcccttcagt
    11761 cttcggtaca gaaatgtagt tttcgatatc aacggtatcg aggatctgac caccaaaacg
    11821 ttcgcccatc agaccggtac ggatgccttt acgtgcggag taaattgctg ccgctgcacc
    11881 cgccgggccg gaaccaacga ttaatacgtc ataagcatca cgcttgttca gctcttctgc
    11941 cgcacgattt tccgcgccag tatcaatttt ggcaacgatt tcagtcaacg tcatgcggcc
    12001 ctgaccaaac tctttcccgt ttacgaacac tgccggaacg cccatcacgt tgcgatcggt
    12061 gatttcgttc tggaaggtgc cgccgtcaat tgcagtgtgc ttgatgcgcg ggttcaatac
    12121 gctcatcagg ttcagcgcct gcaccacgtc cgggcagtta tggcaagaga gcgagtaata
    12181 ggtttcgaat tcaaaatcac cgtcaatatg gcgaatctgc tccagcagag actgcgcttc
    12241 tttcgacgga tgaccaccgg tccacagcaa cgccagtacc agcgaggtga actcgtggcc
    12301 cagcggggag cctgcaaaac gtggtccctg gttggaacct gggttggtga tcaggaaaga
    12361 cggcttacgc actggcaagc tgttatcttc tttaaaggtg actttgtctg acagttctgc
    12421 gatttcagcc aacagttcct tgatttctgc cgatttagcg ctgtcatcca gcgtggcaat
    12481 taactcaaca ggcttggtca atttctcaag gtaagccttg agttgagttt tcatatttgt
    12541 gtcgagcatg tttatctcct gggctttaaa catcatcatg caagcggcct ctgcggctac
    12601 ctggatgcag cttgcatcat ggtgcgggga agaaaatcgg gtgccgcagc acccgaagaa
    12661 ttaggtgaat ttccgaagta tttcgcgtca gagcaaggcg actagcctgt gaatccccgg
    12721 gagcttacac aagtaagtga ccggggtgag cgggcgacgc caacgccgct atggcgtgaa
    12781 agacgaagga aatttagatt ttaccaacca ggtccagaga cggagccaga gttgcttcac
    12841 cttctttcca tttagccggg caaacttcac ctgggtgaga agctacgtac tgagctgctt
    12901 tgattttacg cagcagatca gacgcgtcac ggccaatgcc ttcagcggta acttcgattg
    12961 cctggatgat accctgcggg tcaacaacga aggtcgcacg gtcagccaga ccttcatctt
    13021 cacgcatgtt gtcgaagtta cgggtcaggg cgccagtcgg gtcgccgatc atcgcatatt
    13081 tgattttagc gatggtttca gagctgctgt gccatgcttt gtgggtgaag tgagtatcgg
    13141 tagatactgc gtatacgtct acgcccagtt tctgcagttc ttcgtagtgg tcagcaacgt
    13201 cacccagttc ggtcgggcat acgaaagtaa agtcagccgg gtagaagaag aagacgctcc
    13261 agcggccttc ggtatctttt tcggtgattt cgatgaattc gccgtttttg aatgcctggt
    13321 ttttaaaagg tttaattttg gtgttaatca aggacatcta tacttcctcc gtgttttcga
    13381 tgagatgtaa ggtaaccaat ttttgccgat tcggctaatg cgtttccatt atcaaatcga
    13441 taagttttac cttacaacct tcgtaagaca actttgtgaa ctttgcaccg ccagcggcga
    13501 taaaaagtaa aatggccccc tttccaggtg accgtgtaat ctgaactacc ctttggtatc
    13561 ttcagcgcct gcctgcaggc tgaggtgttg cccttacaaa tgcaacaacg acatggatta
    13621 caacaccctc ataaacaaag ggcaatcacc tgatctaagc tcttacctat gacagtgata
    13681 ggttatgcct tttactcgac ttttgcactg actgaaaaga acaaattaat gttaaaaaag
    13741 atacttttac tggctctgct tcctgcaatc gccttcgcag aggaacttcc ttccccagta
    13801 aaagcgattg aaaaacaggg cattacaatc atcaaaacgt tcgatgcccc cggaggaatg
    13861 aaaggttatc tcggaaagta tcaggatatg ggcgtcacca tctacctgac tcccgatggt
    13921 aagcacgcta tctctggtta catgtacaac gagaaaggtg aaaacctgag taacacactt
    13981 atcgaaaaag aaatttacgc accagccgga cgcgaaatgt ggcaacggat ggaacaatcc
    14041 cactggctcc tcgacggtaa aaaagatgcg ccggtcattg tctacgtctt ctccgatccg
    14101 ttctgcccat attgtaaaca gttctggcag caggcgcgcc cgtgggtaga ttctggcaaa
    14161 gtgcaattaa gaacattgtt ggttggggtt atcaagccag aaagcccggc gacagcagcg
    14221 gcaattcttg cctccaaaga tcccgcaaaa acctggcaac aatatgaagc ctctggttgc
    14281 aagcttaagc taaacgtgcc tgcaaacgta agtacagagc aaatgaaagt gttaagtgac
    14341 aatgagaaac tgatggacga tctgggggca aatgtcacgc cggctatcta ttacatgagt
    14401 aaggaaaata cgctgcaaca ggccgtgggg ttgcccgatc agaaaacgct taatatcatt
    14461 atgggaaata aataaaaggt aattcatgga tggttgaact atatccctgc ttttcaacca
    14521 tcctaaaaaa gcatgaattt ctcgctctac cctaagtggc tgtgctattt tagaaaaact
    14581 taatcctgag ttaagcaaaa aaacataatc aataaaaata catacgcaaa aaacattgat
    14641 taagtgaata taccatggaa gaaaaatata aaccggagta gtgtatggcc aatctctacg
    14701 acttgaaaaa gctcgactta aatcttcttg tcatatttga gtgtatttac cagcatttaa
    14761 gcattagcaa agctgctgaa tcactatata tcaccccatc agccgtgagc cagtcgttac
    14821 aacgcttacg tgcgcaattt aatgaccctt tattcattcg ctcaggaaaa ggtatcgcgc
    14881 cgacgacaac agggttgaat ctgcatcacc atcttgagaa aaacctcaaa ggccttgaac
    14941 aaacaattaa tatagtcaat aagtccgaac taaagaaaaa ctttattatc tacggccctc
    15001 agctcatttc ttgctctaat aatagtatgc tgatccgctg cctacggcaa gatgcctctg
    15061 tcgagattga gtgccatgat atcctcatgt cagcagagaa tgctgaagaa ctgttggttc
    15121 accgcaaagc cgatctggtg atcacccaaa tgccagtgat cagccgttcc gtcatttgca
    15181 tgccattgca tacaattcgc aatactctaa tttgtagtaa taagcatccg cgaattactg
    15241 acaatagcac ttatgaacaa attatggcag aagagttcac ccaattaata gccaaatcag
    15301 caggagttga tgatattcaa atggaaatag acgagaagtt tatgaatcgc aaaatttcat
    15361 ttcgtggttc ctctttgctg acaataatta atagcattgc agttacagat ttactgggaa
    15421 tagtccccta tgaactttat aactaccacc gtgactttct taatttaaaa gagataaaac
    15481 tagaacatcc gttaccttca ataaagcttt acatttccta taataaatct tctttaaata
    15541 atttggtgtt ctcccgattt attgaccgtc taaatgagag tttttgatat ataactatag
    15601 acagctacta taactttcat ctatttattc acagcgcgaa tgaaaaataa aacactctgc
    15661 tttacagtgt ttactttata attaacagtc ttatctcact gttcaccctg taacgttcag
    15721 agagcgtctt cagatgtcta tttataaaat tcctcttccg ctcaatattc tggaagctgc
    15781 acgagagcgt attacctaga cactcaacac cctaccccgc gtttgtgtct cattttctgg
    15841 cggcaaagac tctggtttaa tgctgcatct gacagccgaa cttgcccgac aaatgggcaa
    15901 aaaaatctgc gttttgttta tcaactggga ggc
//
LOCUS       DAETQL010000087        15439 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.087, whole genome shotgun sequence.
ACCESSION   DAETQL010000087 DAETQL010000000
VERSION     DAETQL010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.087"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="K0W65_003157"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="K0W65_003157"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBI3096316.1"
                     /translation="MKALSKLKAEEGIWMTDVPVSELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="K0W65_003158"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="K0W65_003158"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBI3096317.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="K0W65_003159"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="K0W65_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HBI3096318.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="K0W65_003160"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="K0W65_003160"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBI3096319.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="K0W65_003161"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="K0W65_003161"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBI3096320.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="K0W65_003162"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="K0W65_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBI3096321.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="K0W65_003163"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="K0W65_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HBI3096322.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="K0W65_003164"
     CDS             complement(8064..9206)
                     /locus_tag="K0W65_003164"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HBI3096323.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="K0W65_003165"
     CDS             complement(9215..10228)
                     /locus_tag="K0W65_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HBI3096324.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="K0W65_003166"
     CDS             complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="K0W65_003166"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HBI3096325.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="K0W65_003167"
                     /gene_synonym="rfaI"
     CDS             complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="K0W65_003167"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HBI3096326.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="K0W65_003168"
     CDS             complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="K0W65_003168"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBI3096327.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12826..13950)
                     /locus_tag="K0W65_003169"
     CDS             complement(12826..13950)
                     /locus_tag="K0W65_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBI3096328.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="K0W65_003170"
     CDS             complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="K0W65_003170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBI3096329.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            complement(15363..>15439)
                     /locus_tag="K0W65_003171"
     CDS             complement(15363..>15439)
                     /locus_tag="K0W65_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3096330.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         4407 a         3466 c         3165 g         4401 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccgatac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt
    10861 taaccctgca agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta
    10921 tgaaaacttt caacccattt atcgttttat tatatatcat aatgaattat tttaacctta
    10981 aatctttaga agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa
    11041 tgcttttgtt taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta
    11101 agtaaatctt ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc
    11161 ggataatcag cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg
    11221 tcatcattga ctggattatc aactttgtct tttaattcat agttaatact atatcgggta
    11281 ttatatttct ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga
    11341 tcaaggtggg tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac
    11401 gaaatgttat ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat
    11461 ccagaagcta actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct
    11521 ctttcagcta caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta
    11581 atactaccct tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag
    11641 aaataatctg caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt
    11701 aatgacttca gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt
    11761 gctaaatcag aaaaatataa tttgtcttta tcactgatat aatctgtgaa aacatgaaat
    11821 tcacaagaga tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa
    11881 agaaaatttc tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga
    11941 taatgaaaat tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt
    12001 ctgtatccaa aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt
    12061 tccctgattt ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga
    12121 atgtctttaa gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc
    12181 aggccaatat tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct
    12241 ggaacgcgcg tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct
    12301 tccttaccgg aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta
    12361 atgcccgcag catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt
    12421 acgcgaacat ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt
    12481 ggtgtcagat cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg
    12541 ccgaatgcca ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc
    12601 cattcgcggt ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct
    12661 ttcagggtcg tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc
    12721 agagtacggc gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca
    12781 aaaggatctt tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc
    12841 acccgttatg atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata
    12901 atgtcgcgca ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa
    12961 aatctcattc aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc
    13021 gacaatataa tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat
    13081 cgcttccagc agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc
    13141 cgccgccatt aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac
    13201 gccgcgtttt tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag
    13261 caatgtgttg tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc
    13321 acctttacgc gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc
    13381 attcttctta cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg
    13441 ataaatcccc ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa
    13501 atcggcgatt tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt
    13561 tgcccgctca aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt
    13621 ttcctgcgct actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg
    13681 cattttgttg aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca
    13741 ggcaaaatac tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac
    13801 ttcaaataca tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc
    13861 tcgggcggcg actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa
    13921 atatttatat aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt
    13981 aatttatcga cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt
    14041 cggtcacgct gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg
    14101 ttatttgacc agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc
    14161 gtattaactg cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca
    14221 tgatcgatta acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt
    14281 ggcgtctggc atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg
    14341 gacgtcagaa cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg
    14401 gcgttgtccc agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt
    14461 ccaacaccag cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc
    14521 atggttgtct gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt
    14581 tccaccacat ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca
    14641 gactgccgat gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt
    14701 atagcaacca tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat
    14761 accttgatga gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt
    14821 atgccgtaga gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa
    14881 agcagcacat cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta
    14941 gttaataaca tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta
    15001 tccacaagta actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct
    15061 accgccccag gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt
    15121 gtatcacttc tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca
    15181 ataaagaaat caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga
    15241 ttccttcctg cacgtacagc agaaatcaat gaatcgcata aaaatacaaa aaaagtatag
    15301 atgttcaaca agaattggcg ggtaaattgc tatttaagtc aaaaacgggt aatgactcca
    15361 acttactgat agtgttttat gttcagataa tgcccgatga ccttgtcatg cagctccacc
    15421 gattttgaga acgacagtg
//
LOCUS       DAETQL010000088        15412 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.088, whole genome shotgun sequence.
ACCESSION   DAETQL010000088 DAETQL010000000
VERSION     DAETQL010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.088"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1177)
                     /locus_tag="K0W65_003172"
     CDS             complement(<1..1177)
                     /locus_tag="K0W65_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBI3096331.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1376..1460)
                     /locus_tag="K0W65_003173"
     tRNA            complement(1376..1460)
                     /locus_tag="K0W65_003173"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1424..1426),aa:Leu,seq:caa)
     gene            1656..2675
                     /gene="ahr"
                     /locus_tag="K0W65_003174"
     CDS             1656..2675
                     /gene="ahr"
                     /locus_tag="K0W65_003174"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBI3096332.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2804..4306
                     /locus_tag="K0W65_003175"
     CDS             2804..4306
                     /locus_tag="K0W65_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBI3096333.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="K0W65_003176"
     CDS             complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="K0W65_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBI3096334.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="K0W65_003177"
     CDS             complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="K0W65_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBI3096335.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6874..8385
                     /gene="pepA"
                     /locus_tag="K0W65_003178"
     CDS             6874..8385
                     /gene="pepA"
                     /locus_tag="K0W65_003178"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBI3096336.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8519..8962
                     /gene="holC"
                     /locus_tag="K0W65_003179"
     CDS             8519..8962
                     /gene="holC"
                     /locus_tag="K0W65_003179"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBI3096337.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8962..11817
                     /gene="valS"
                     /locus_tag="K0W65_003180"
     CDS             8962..11817
                     /gene="valS"
                     /locus_tag="K0W65_003180"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBI3096338.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11871..13067)
                     /locus_tag="K0W65_003181"
     CDS             complement(11871..13067)
                     /locus_tag="K0W65_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HBI3096339.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13260..13763
                     /locus_tag="K0W65_003182"
     CDS             13260..13763
                     /locus_tag="K0W65_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI3096340.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="K0W65_003183"
     CDS             complement(13809..14225)
                     /gene="rraB"
                     /locus_tag="K0W65_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBI3096341.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14387..15400
                     /gene="argF"
                     /locus_tag="K0W65_003184"
     CDS             14387..15400
                     /gene="argF"
                     /locus_tag="K0W65_003184"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBI3096342.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         3978 a         3933 c         3969 g         3532 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tccgatggtg
     1381 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1441 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1501 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1561 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1621 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1681 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1741 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1801 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1861 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1921 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1981 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     2041 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2101 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2161 ccagccgcgt tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     2221 acgcaatggg atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac
     2281 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt
     2341 cggggcagtt tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt
     2401 ttgaggcgct gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     2461 ctgttccggc ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca
     2521 cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa
     2581 ccgaactgtt cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag
     2641 cgcgctaccg agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg
     2701 gcacttaaat ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca
     2761 cagccgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg aacccctgtt
     2821 aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta accgtcacgg
     2881 gctgattact ggcgcaacgg ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc
     2941 attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg aaaggcgatc tgaccggtat
     3001 cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg cttaaaaata tcggcgtcaa
     3061 tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg agaaaggcca
     3121 cacggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcgcgac tgttgaatct
     3181 caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg atcagggatt
     3241 gttgctgctc gactttaaag atttgcgggc gattacccag tacatcggcg ataacgccaa
     3301 atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg
     3361 attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga tgctggatat
     3421 caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc tcagcgccga
     3481 gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc tttcagagtt
     3541 gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt ttttcttcga
     3601 cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga ttgagcaggt
     3661 gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga
     3721 tattccggat aatgtgttcg gccagctcgg taatcgtgtt cagcacgctt tgcgggcttt
     3781 tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa accatgcggg ccaatccggc
     3841 atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa tctcgtttct
     3901 cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg
     3961 aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactccc cggtgtatgg
     4021 caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa aaggctttca
     4081 ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg aaagaggtgg cggtggatga
     4141 cggtattctt ggtggattga aggatatttt gtttggcact accggaccac gcggcgggaa
     4201 gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga cgaatcagat
     4261 tgtacgcggg atgttgggga gtttgctggg ggggagaaaa agataagtca gacgtgatct
     4321 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4381 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4441 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4501 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4561 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4621 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4681 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4741 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4801 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4861 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4921 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4981 ctgatgccac ctaacacctc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
     5041 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5101 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5161 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5221 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5281 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5341 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5401 acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
     5461 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5521 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5581 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5641 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5701 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5761 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5821 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5881 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5941 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     6001 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6061 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6121 tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6181 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6241 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6301 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6361 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6421 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6481 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6541 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6601 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6661 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6721 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6781 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6841 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6901 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6961 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     7021 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7081 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7141 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7201 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7261 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7321 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7381 ggtgagcgcg cgatccagca cggtcaggcg attgccgccg ggataaaagc agcgaaagat
     7441 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7501 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7561 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7621 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7681 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7741 atgaagtacg atatgtgcgg cgcggcggca gtttacggcg tgatgcgtat ggtcgcggag
     7801 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7861 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7921 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7981 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     8041 catcatatta ctggtctgat gacgaaccat aatccgctgg cccatgaact gattgccgcg
     8101 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8161 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8221 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8281 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8341 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8401 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8461 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8521 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8581 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8641 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8701 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcagtg cgccggtgga
     8761 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8821 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8881 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8941 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     9001 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9061 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9121 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9181 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9241 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9301 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9361 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9421 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9481 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9541 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9601 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9661 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9721 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9781 ggctgcgtga agatcacacc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9841 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9901 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9961 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
    10021 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10081 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10141 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10201 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10261 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10321 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10381 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10441 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10501 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10561 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10621 aagatgtcca aatccaaggg taacgttatt gacccgctgg atatggttga cggtatttcg
    10681 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10741 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10801 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10861 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10921 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10981 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    11041 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11101 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11161 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11221 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11281 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11341 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11401 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11461 gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc
    11521 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11581 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11641 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11701 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt
    11761 tatgcagaag cgaaagccaa gctgattgaa cagcaggctg ttatcgccgc actgtaatca
    11821 acacagtgat gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat
    11881 ggtaaagaag gcataactcc ccgagaaata ctggctaaga agcctttatc tggttgttct
    11941 tctttattga ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc
    12001 cagtcaatca tccatatttt cagcagtggg taagccagac cacccgtaat cccggatatc
    12061 accaccaacg cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga
    12121 atacgcccat cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa
    12181 taactggtgc taacagcaat cccaaaataa taaattaact gcgcgattat cattttacgt
    12241 tgcatttcca taaattgctg taaattctca atatcatcat ttgcataccc agaactatca
    12301 tactcattta atatttgatc gaagataata taaccagcaa cagcgagaaa aggtaataaa
    12361 gctaaaaatg cgaggatggc atattttata caatacgcag tatctaattt caccttgaaa
    12421 cgatgtatac cgaaactggt attgctccag agaaaactca ttaccaggct atataaagta
    12481 ccattaaaaa taccaatgga aacaatacca actattgcca tcagaaccac agatataata
    12541 acactacttg aactattggc atgtagcatc tttgtcgaga taaagaaaac agtccccatc
    12601 ccaatggcca ttaaaatcgg caagaaaaag gttacccacc agaacccttt caaagagcaa
    12661 ttaaaactaa atcttacgcc gttgagactg gtcattaagg cctgataacg cagtcctttt
    12721 gctgccataa atataagcaa aaccaacagt aacaaagtca aaacacagcc aacaagcggc
    12781 atatctgctg acactgtcat taagattgcg aaatagaaaa aaacaaaaac aagacaacta
    12841 acaaaaacat tcccaccggt aattccataa gaaaatcgtt gtccgttaac ttccatatta
    12901 gcataaagat aacgcttaca tttcattaat gcccatggta aatagatacc tagcgttata
    12961 atcgttaaca acacattcac cacacaaata agaaaatatc tctcacctgt accatgaaaa
    13021 acaaaagaat gggacggaac atccatttca ttaataactt gagccataaa aatcccctga
    13081 attaaaaaat ccattgatat aatttataga tacagacagt aaaatgttct gccacgttac
    13141 aatactctta tcagtaatag gctggcaaaa actttattgc tttataagac gcttgcgcac
    13201 gtaagaagag agtggtatta aacaaatgta taattacttt atttttattg ggttaaacta
    13261 tgaataacat tgcgccgcaa tcacctgtaa tgcgtcgcct gacgctgcag gataatcctg
    13321 ctatcgccag cgtcatccgt caggtttacg cagagtatgg tcttaccgcc gataaaggct
    13381 acaccgttgc cgatccgaat ctcgacgaac tgtatcaagt gtacagccag cctggccatg
    13441 cttattgggt ggttgaatac aatggcgaag tggtcggcgg cggcgggatt gcgccattaa
    13501 caggtagtga agcggacatt tgcgaactgc agaaaatgta ctttcttccc gctattcgcg
    13561 gtaaagggct agcgaaaaaa ctggccttaa tggcgatgga gcaggcacga gagatgggct
    13621 tcaaacgctg ctatctggaa acgaccgctt ttttaaagga agccattgcg ctttatgagc
    13681 atttgggctt tgagcatatc gactatgcgc ttggctgcac gggccatgtc gattgtgaag
    13741 tgcggatgct acgcgaactc taaaaaaatg cccgctagta tgtgctatcg ggcattttaa
    13801 tcaattaatt agtggcgaac cccgtcatcg tcttcatcga caaaatcttc atcgtcgcca
    13861 tcttcgccgt taggatcttc aaagtaagtg ccccaaccgt cgtactcaac gtcaaatttc
    13921 tccgccagcg tcatcagttg ttcaacctgg gcatcgatca gatcggcatt caacgcgcac
    13981 tcgctgagga tgtcgcagca aatcacgata tcaccatctt caacttccag ctcttctgga
    14041 tcggtcactt cgtaaccgag tttaaacgct tcaactgccg ctttttccag agtttccaga
    14101 tcgtctgcgg aaagatggtg ttcgatggtg tacagcgcgt ccggatcgct gccatcttcc
    14161 agtaattctt caataatcaa acgtgtttct tcacgctgtt cttccagttg ttccgggttt
    14221 gccatggctc attcctctta atgtcctgcc gatactctta ttgtcacaca ccactgacat
    14281 tgcctccacc tttgtgacaa agatttatgc tttagacttg caaatgaata ataatacaca
    14341 taaattgagt tttaattcat tgaggcgtta gccacaggag ggatctatgt ccgggtttta
    14401 tcataagcat ttcctgaaat tactcgattt cacgccagct gaactcaaca gcctgctgca
    14461 gttagccgcg aagctgaaag ctgataagaa aagcggtaaa gaagaagcca aactcactgg
    14521 taaaaacatc gcgctcatct tcgaaaaaga ctcgactcgt acccgatgct ctttcgaagt
    14581 tgccgcatat gaccaggggg ctcgcgttac ttatcttggc ccaagcggta gtcagattgg
    14641 tcataaagag tcgattaaag acactgcccg cgtgcttggt cgcatgtatg acggtattca
    14701 gtatcgcggc tatggtcagg agattgtcga aacactggcg gaatacgctg gcgtgccggt
    14761 atggaacggc ctgaccaatg agtttcaccc cacgcagttg ctggcggatc tcctcaccat
    14821 gcaggagcat ttacccggta aagcattcaa cgaaatgacg ctggtctatg caggtgacgc
    14881 acgtaacaac atgggcaatt cgatgctcga agctgcggcg cttaccggtc tggatttgcg
    14941 tctggtcgcg ccacaggcat gctggccaga agctgcgctg gttacggaat gccgcgccct
    15001 ggcacaacaa aatggcggga atattacgct gacggaagat gtagctaaag gagttgaagg
    15061 tgctgacttt atctataccg atgtatgggt gtcgatgggt gaagcaaaag aaaaatgggc
    15121 tgaacgcatt gcattactgc gtgattatca ggtgaacagc aagatgatgc agttgaccgg
    15181 taacccggag gtcaaattcc tccattgcct gcccgcgttt catgacgacc aaacgacgct
    15241 tggcaaaaaa atggcggaag agtttggcct acatggcgga atggaagtga ctgatgaggt
    15301 cttcgaatct gccgccagca ttgtatttga tcaggcggaa aaccgcatgc ataccatcaa
    15361 agcggtgatg gtcgcgacgc tcagtaaatt aaataattaa tatcgctata aa
//
LOCUS       DAETQL010000089        15112 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.089, whole genome shotgun sequence.
ACCESSION   DAETQL010000089 DAETQL010000000
VERSION     DAETQL010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.089"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..111
                     /locus_tag="K0W65_003185"
     CDS             <1..111
                     /locus_tag="K0W65_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3096343.1"
                     /translation="LRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(653..1594)
                     /gene="fabY"
                     /locus_tag="K0W65_003186"
                     /gene_synonym="yiiD"
     CDS             complement(653..1594)
                     /gene="fabY"
                     /locus_tag="K0W65_003186"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBI3096344.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1639..2076)
                     /gene="dtd"
                     /locus_tag="K0W65_003187"
     CDS             complement(1639..2076)
                     /gene="dtd"
                     /locus_tag="K0W65_003187"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBI3096345.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2073..2945)
                     /locus_tag="K0W65_003188"
     CDS             complement(2073..2945)
                     /locus_tag="K0W65_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBI3096346.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2939..3538)
                     /gene="yihX"
                     /locus_tag="K0W65_003189"
     CDS             complement(2939..3538)
                     /gene="yihX"
                     /locus_tag="K0W65_003189"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBI3096347.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3637..4422)
                     /locus_tag="K0W65_003190"
     CDS             complement(3637..4422)
                     /locus_tag="K0W65_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI3096348.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4456..5352)
                     /gene="yihV"
                     /locus_tag="K0W65_003191"
     CDS             complement(4456..5352)
                     /gene="yihV"
                     /locus_tag="K0W65_003191"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HBI3096349.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5520..6416
                     /gene="yihU"
                     /locus_tag="K0W65_003192"
     CDS             5520..6416
                     /gene="yihU"
                     /locus_tag="K0W65_003192"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBI3096350.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6440..7318
                     /gene="yihT"
                     /locus_tag="K0W65_003193"
                     /pseudo
     CDS             6440..7318
                     /gene="yihT"
                     /locus_tag="K0W65_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
     gene            7335..8576
                     /gene="yihS"
                     /locus_tag="K0W65_003194"
     CDS             7335..8576
                     /gene="yihS"
                     /locus_tag="K0W65_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HBI3096351.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8714..9616
                     /locus_tag="K0W65_003195"
     CDS             8714..9616
                     /locus_tag="K0W65_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HBI3096352.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9815..11851
                     /gene="yihQ"
                     /locus_tag="K0W65_003196"
     CDS             9815..11851
                     /gene="yihQ"
                     /locus_tag="K0W65_003196"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HBI3096353.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11897..13271
                     /locus_tag="K0W65_003197"
                     /pseudo
     CDS             11897..13271
                     /locus_tag="K0W65_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13314..14717
                     /locus_tag="K0W65_003198"
     CDS             13314..14717
                     /locus_tag="K0W65_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3096354.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14785..>15112
                     /gene="ompL"
                     /locus_tag="K0W65_003199"
     CDS             14785..>15112
                     /gene="ompL"
                     /locus_tag="K0W65_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HBI3096355.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3717 a         3883 c         3768 g         3744 t
ORIGIN      
        1 ctgagacaac atctggcaag gctgggacgg aagtcactgt cgttctcaaa atcggtggag
       61 ctgcatgaca aggtcatcgg gcattatctg aacataaaac actatcagta agttggagtc
      121 attacctgaa aattgattta atagcgtttg cttcggtgat ttattactca ttagttgttt
      181 ttctaaatta acaacagaat aactttcttg tttaatgaga ttaatattgg caaccattat
      241 agcctccttc ccacggttta agagcaaaca tacaacataa tgattcataa catacagagc
      301 agtaaatgaa tgatactgac aatgtatgcc agcccattat ctttgcataa tatttgtgag
      361 ctatagattt taaaaatatt tatgaaaaaa gtatggcacc agagggggtt ttgactatca
      421 aaaatcgctc aaacctgaac taatatgatg agttaagcat tatctgcaat aattgccact
      481 cagctctaca aataatattt aaatgctcct gaacagtgat gtagcaagac acgtatggtg
      541 ccaacgaaga ataaaaattt ataaagttct tattcaatat aaccggaata gtcagggact
      601 gatgtaaatg accggaatga atgagagagg cagcaccgcc cctctccata agctactctt
      661 cttcgttccc gccctcttca tacgggccaa atggcttcgc gggcagaacg atatacgtgc
      721 cttcaaacac tgcacccggc gtctcgtcgc caaagatttc gacctgcatc tgcacccgtg
      781 cttttcgtcc gcgcgccaga cggtcgagat cgccgcttaa ggcaccaagg tcggctaccg
      841 catggggttt accgctaatc ggtttgctgt agcggatatg cgcatccgcc agaataatcg
      901 ttccgccgag gtggcgttca cgcagcatca gccagataag cccccaaccg gtgagcgtcg
      961 ccagcgagaa taaactcccg gcaaacagcg tatggtgcgg attctgattg ccggtttccg
     1021 gcatggtggt gataaatttt tgcccggtat attgttgaat acgcacgccc attttttcac
     1081 taagcgggat atgttcgtac cacgcctgtt gcagctgcgc gcaccagtcg ccgcgatgca
     1141 aaatatcatc cagagtggcg acgggcttaa tcatcaaaaa atggcgaatc ggcgtggtgg
     1201 ttggcgtggt gatttctccc tgattaacaa accccagctt ggcgaaaaac tccaccgcgt
     1261 cttcacgggc gctacaggtc acgcgcttaa cgccttcctg acgcgccacc gactccaggg
     1321 tcatcgccat cagcgttcct aaccctttgt cctgcacgtc cgggtgaacg accataaagc
     1381 gaatggacgc ttcattgtcg gcattaatat acaatcggcc taccgccacc agattaccct
     1441 gctcgtcgac gaccatctgg tgatgcgcca tcgcatccca cgcgtcgcgt tccgaacctt
     1501 ttggttgatg caggggctta cgcaacattt cccagcgaaa gtgatagtaa cgctctaact
     1561 cttcttctgt ttgtggaacc tgaaggtgat acatagcgat actctctctt gtttcccgtg
     1621 acaaccctgg aagctggctc atacctgcaa ccagaatgtc acggggccat cattgaccag
     1681 cgatacctgc atatcggcag cgaagcgtcc tgtttgcgtg ttcatctctt gctggcggca
     1741 gcgttcgacg aaatagtcat ataacgcctc tgcgcgatcc ggtgatgcgc ctttggagaa
     1801 acttgggcgc atcccccgtt cggtatctgc ggcgagggta aactgggaaa ccaccagtac
     1861 actgccgccc gcctgttgca cgttgagatt catcttgcct tcggcatcgc taaagatgcg
     1921 gtagccgagc acacgctcgc acagacggtt tgctttctgt tcgtcgtcat ccttttcgac
     1981 acccaataac accaaaagtc ccgcgccaat ttcgcccgtc acttctccct ccacggtgac
     2041 gctggcacgg gttacgcgtt gaattaatgc aatcatggtt cgtcgtcttc ttcttgttca
     2101 gctgcttgtt ttagtttgcg gtattccccg agagtgacag taatttccgc gccaagcaag
     2161 acgatacacc atgtccagta gacccaaaca aagagaatgg ggatcaccgc cagcacaccg
     2221 taaatgagct gatatgacgg gaacatggtg atataaagcg cgaaaccttt ctttcctgct
     2281 tcgaacagga gtgcggcgac aaacgcgccg acaatcgcgt cgcggttagg tacgcggatg
     2341 gtaggaacaa tgctgtacag caaccagaag gagatccacg acaacagcaa cggaaaaata
     2401 cgcagcacgt tgtcgatgac agtattgaga tcgctcgccc agcgcaggga gagcaaatag
     2461 gaactgatcg ccagactggc ccctgccagc agcggcccca gcgttaaaat catccagtac
     2521 acggcgaacg agtaaatttt tggtcgcgct cgtttactgc gccagatggt attcaacgcg
     2581 ctatcgatgg agtacatcaa caataacgcg gtgacgatca gcccgcacgc cccaacggcg
     2641 gtcatcttgt tggaattagc aacaaattgt tcgatatacc gctgaataac atcgccagta
     2701 gcaggcagaa agttggcaaa aataaagtga cgtaactgaa tgctgacgtc ggaaaacatg
     2761 ggaaaagcgg cgaaaagcgc aaaaacaacg gcaaccagcg gcactaatga gagcaacgac
     2821 acataggcaa ggttacctgc cagggttgtc atgttgtcct catcaatacg ttgccagagt
     2881 agttttagcc aggcccatag tggacgggta cggtgcctgg ctttgtcctg aatggttttt
     2941 agcataacac cttcgcgaaa tagtccggga tggtggtttt atctttcacc agaatactgg
     3001 taatgcctag ctgattagct ccttctatat tatcggcgtt atcgtcgaaa aagaccgtat
     3061 cgctgggtga aaaaccttcc gcctgcaaaa catgctggta aattcgtgct tcaggtttgc
     3121 gcatccccag atcttgcgac agatagatat ggtcagcagc atcacgaatt tccgggtatt
     3181 cttccggcca gaaggtggta tgcaggcggt tggtattgga aagcaccacc acgcgatgcc
     3241 cctgctcacg cagtttatgc atgatggcga tcacttccgg gcgcagcgca acaaacaccg
     3301 cctgccagcc gtgagagaac tgctcgtagc ttagcggtag agccatctca tgacacagcg
     3361 cctctgcgaa cgcttcgtcg ctaatttccc cacgctcatg ctgatgaaac gcctccccca
     3421 tatgaaaact cttcttaagc gatgccagcg gaatacgcgt taaatcgctc caggctccca
     3481 gcacacggtt aaagtcgata tcgacaatca cattacctaa atcaaagata tagagcattt
     3541 ttgcctcctt tcgcgccaca agaaattaac tgtagcggga aaggatgggt ttggctatgt
     3601 ggcaaggtat gaagccagat taacggtcac aaaaatctac gcgtcttcct ggggaagaat
     3661 gatttcaatt tggctgtctt gtaagcgtga ttttagcgca gcgggcggca gctcgtcggt
     3721 aaagagcgca gtgacctgtg ccacgttacc aatttcaacc gccgccgaag catgatactt
     3781 agtgtgatcg gcgaccagca gaatatttct cgcgtgcgcc atcatcgttt tcaccacgtt
     3841 agcttcgttt acatcaaact ccatcaacgc gccatcgctc tcaatcgccc caacgcttgt
     3901 taccagataa tcagcacgaa aatcagccac aaaggacgcc gctgaagggc caatgatccc
     3961 gctattatga gagcgcaacg taccgccggg caccatcact tcaaagcgcg ggttgtggta
     4021 aagaatatgc gccacacgca ggctgttggt gattatccgc aaatgattat ggttaagtaa
     4081 cgcccgggca acatgctcaa cagtcgtacc aatggtgata aatattgttg aaccatcagg
     4141 aatatagtct gccaccgctt cggcaatcgc tttttttttc tcggtttgcg aaacctcacg
     4201 ctgctcgaac gccgtattaa cgacgctgga agcccgaccc gcgccaccgt gatggcgcgt
     4261 aatcaggcct tgctcgctta atttacgaat atcccggcgg accgtctgcg tggagacatc
     4321 cagcagattt gccagctcat caatattcat atacccacgc tcggcgatca gcatgaggag
     4381 ttggtcgtgc cgcgggttac cggttagttc ggtaaggctc atgaaaattc ctcgaaaaac
     4441 catcaccctg gcattttata caaaaagtga caaaaaagat cgggtttgat cacagtcagg
     4501 gatcccggct cgtccaccgg gacgtgtgca ttttaacgcc gctacaccgc tggcgaagcg
     4561 gactgactcc gctaaatccc cacttgttgc cagcgccacc gccaaagcgc cgtgaaaaac
     4621 atctcccgca cctgtggtat ctaccacatc aactttgaag gccggttgat gctgacgccc
     4681 accattttcc agccagtcgc agcctgcgct accctgggtc acatagacat gaccatttgt
     4741 gagcgtttgt gcctgtttta gcgcactggc catctctttc acgcccgtta agcgcgccag
     4801 acccggttct gaaaaggccg cgtgatcgct taatgccacc agctcactga tatcctgcgg
     4861 cgtaatgtcc ccgtccagaa cggtcatcac acccgcctga cgggccaggg tgaaggcttt
     4921 tttagcgccg tcgtgccagc gtacatctgc cagcacaaca tcccactgag agaaatcaat
     4981 ttcctccaac cactctgcgt caggcagcag atccgggctg gggtagttaa tgattatccg
     5041 ctcgcctttg gtatccacca tgatggcgga ttgcgaagat ttcgcctggt tataccgttt
     5101 ggtgtaacgg gtgttaacgc cccaggattc cagctctgcc agcaggctgt tgcccgcgtc
     5161 gtcatcacct acgcgaccaa taaaatcgac ctgcgccccc agccttgccg ccgcaaccgc
     5221 tgccgtcgcc gctggcccgc caccaacttc cgtataattt ctcgccacgt atttaccgct
     5281 ctccgtcggt aacccttcca cgtaatagat gcgatccatc acggttatac ctacacaagc
     5341 aacacgaatc atggtcattc cttaaacatt ttgggatgcg ttcattttaa tttcaccaat
     5401 tgtttaaaaa gtgacggctg tcaatttttt gacgataaat tacaaattcg atcaaaaaca
     5461 gacaagcaaa gcatgtcata aatgacaaaa agtgacatca ctgtattcag gagagggtta
     5521 tggcagcaat cgcgtttatc ggtttaggac aaatgggttc gccaatggcg agcaatttat
     5581 tgcagcaagg gcatcaactt cgcgtctttg atgtgaacgc cgaagctgtg cggcatctgg
     5641 tagacaaagg tgcgactccc gccgccaacc cggcgcaggc cgctaaagat gccgaattta
     5701 tcattaccat gttgccgaat ggcgatctgg tgcgcaacgt gttgttcggt gaaaacggcg
     5761 tttgcgaagg cttatctacc gatgcgctgg tcattgatat gtccaccatc catccgctgc
     5821 aaaccgataa attgattgcc gatatgcaag ccaaaggctt cagcatgatg gatgttccgg
     5881 taggccgtac ttctgcaaat gccattaccg gtactctgtt actgctggct ggcggcaccg
     5941 ctgaacaagt tgaacgtgcc acaccgatcc tgatggcgat gggcagtgag ttgatcaacg
     6001 ctggcggtcc gggcatgggg atccgcgtta agctcatcaa caactacatg agcatcgcgc
     6061 tcaatgcgct ttcggcagaa gccgccgttt tgtgcgaagc cctgaatctt cccttcgatg
     6121 ttgccgtcaa agtgatgagc ggtaccgccg ccggtaaagg tcacttcacc acttcctggc
     6181 caaacaaagt cctcagcgga gatctttctc ccgccttcat gatcgatctt gcccataagg
     6241 atcttggcat cgcccttgat gtcgccaacc agctgcatgt gccaatgccg ctgggggccg
     6301 cctcacggga ggtttatagc caggcgcgcg cagcgggtcg cggtcgccag gactggtccg
     6361 ccattctgga acaggtccgt gtcagtgccg ggatgactgc caaagtaaaa atgtaacgac
     6421 tggataaagg aataaatgaa tgaataagta caccatcaac gacattacgc gcgcatcggg
     6481 cggttttgcc atgctggcgg tcgatcagcg cgaagccatg cgcatgatgt ttgccgcggc
     6541 tggggcaccc gctccggtag ccgatagcgt tttaactgat ttcaaagtta acgctgcaaa
     6601 ggccctctcg ccttatgcct cggcgattct ggtagatcaa caattctgct accgccaggt
     6661 ggttgagcaa aacgcgattg ccaaaagttg cgccatgatt gtcgccgccg atgagttcat
     6721 tcctggcaac ggtattccgg tcgatagcgt ggttattgac cgcaaaatca atccgctaca
     6781 gatcaaacaa gacggtggca aagccttaaa actgctggtg ctgtagcgta gtgatgaaga
     6841 tgcgcagcaa cgtctggata tggtgaaaga gttcaacgaa ctgtgccact cacacggtct
     6901 ggtaagcatc attgagccag tcgtccgccc accgcgtcgt ggcgataaat tcgatcgcga
     6961 acaagcgatc atcgatgccg ccaaagagct gggcgacagt ggcgctgacc tctacaaagt
     7021 tgaaatgccg ctttatggca aaggtccgca acaagagctt ctctgtgctt cacaacgtct
     7081 gaatgaccat atcaatatgc catgggtgat cctctcttcc agtgtcgacg aaaaactgtt
     7141 cccgcgtgcc gtacgcgtgg caatgacggc gggcgcatcg ggattcctgg caggccgtgc
     7201 agtctgggca tcggtcgtcg gtttaccaga caacgagctg atgctccgtg acgtttgcgc
     7261 accgaaatta caacaacttg gcgatatcgt cgacgaaatg atggctaaac gccgctaaga
     7321 tgaggataaa aggaatgaaa tggtttaaca ccctaagcca caaccgttgg ctggaacagg
     7381 aaaccgaccg catctttgat tttggtaaaa attccgtagt gccgactggt tttggctggt
     7441 taggcaataa agggcaaatc aaagaagaga tgggcaccca tctgtggatc acagctcgta
     7501 tgttgcacgt ttattccgtt gctgcggcga tgggtcgacc tggcgcttac gcgttggttg
     7561 atcacggtat taaagccatg aacggcgcac tgcgtgataa aaaatatggc ggctggtatg
     7621 cctgcgtgaa tgacgagggc gtggtggatg cctccaaaca gggctatcaa cacttctttg
     7681 ctctgctggg tgccgccagc gccgtcacaa cgggtcaccc ggaagcgcgc aagctgctcg
     7741 attacaccat tgagattatc gagaaatatt tctggagcga agaagagcag atgtgcctgg
     7801 agtcctggga cgaagccttc agcaaaaccg aagagtaccg cggcggcaat gccaatatgc
     7861 acgcggtgga agctttcttg attgtttatg acgtcactca cgacaaaaaa tggctggatc
     7921 gcgcgattcg cgtggcttcc gtgattatcc acgacgtcgc cagaaataat cattatcgcg
     7981 ttaacgaaca cttcgatacc cagtggaatc cgctgccgga ttacaacaaa gataacccgg
     8041 cgcatcgctt ccgcgcgttc ggaggtacac caggccactg gatcgaatgg ggccgtttaa
     8101 tgctgcacat ccatgctgcc ctggaagccc gttgcgaaca accaccagca tggctgctag
     8161 aagatgccaa aggtctgttt aacgccaccg tgcgcgatgc ctgggcaccc gatggagcgg
     8221 acgggattgt ttataccgtt gactgggaag gaaaaccggt ggtccgcgaa cgtgtacgtt
     8281 ggcctatcgt cgaagcaatg ggtacggcct acgcgctcta caccgtcact ggcgatcgcc
     8341 agtatgaaac ctggtatcaa acatggtggg agtactgcat taagtacctg atggactatg
     8401 aaaatggttc ctggtggcag gagctggatg cggacaataa ggtcatcacc aaagtctggg
     8461 acggcaaaca ggatatttat cacctgctgc attgcctggt gatcccgcgt atcccgttag
     8521 cgcccggcat ggctccagcg gttgcagcgg gtctgctgga tattaatgcg aaataactga
     8581 tctgaaaata gggtgcgggt tttcccctca ccctaaccct ctccccaaag gggcgagggg
     8641 accgtccggt gcagtttttg ggtttgacat cagcctcacg ggaggctgta tgtcgttaat
     8701 taaggtgcct tgtatgcaaa tcaccaatat gcattgcagt ggacagaccg tttcacttgc
     8761 cgcgggggat tatcacgcga ccattgtgac cgtcggtgct ggtctggctg aattaacctt
     8821 tcagggttgt catttagtca tcccgcacaa acccgaagag atgccgctgg cgcatctggg
     8881 taaagtgctt attccgtggc cgaaccggat cgccaacggc tgttaccgtt atcaggggca
     8941 ggaatatcag ttacccatta atgaacatag ttcaaaagcc gctattcacg ggctgcttgc
     9001 ctggcgagac tggcaaatta gtgaattaac caccaccagc gttaccctga cagcttttct
     9061 gccgcccagt tacggctatc cgtttatgct ggcttcacaa gttgtttatt cgctcaacgc
     9121 acacacgggt ctttccgttg agatcgcaag ccagaatatc ggcactgtcg cagcgcctta
     9181 tggcgtgggt attcatcctt atttaacctg caacttaaca tctgttgatg aatatctgtt
     9241 ccagcttccg gcaaaccagg tttatgccgt cgatgaacac gtaaatccga caacgcttca
     9301 ccacgttgat gagctggatt taaatttcac gcaggcgaaa aaaatagccg cgacaaaaat
     9361 cgaccacacc tttaaaaccg ctaatgattt gtgggaaatg accattaccc atccacagca
     9421 ggcgctttcg gtaagccttt gttccgatca accctgggtg caggtttata gcggtgaaaa
     9481 attacagcgt cagggactgg cagttgaacc tatgagttgc ccgcctaacg cctttaattc
     9541 aggtatcgac ttattattac tggaaccagg aaaaccgcac cgcctgtttt ttaatattta
     9601 cggacagaga aaataacttg tattgattag cagtcaaaat cattgaagaa catttcactc
     9661 gctaaaggca acattatgta tcttcaattt attaagttaa aaacagctca gcttctgtaa
     9721 agtttttata tatcagtttt atgagaatca ttttacataa gcctgtaata acaggaaggc
     9781 aacaatctcg ttctcaactc tatggagatt agttatggat acgccacgtc cacagttatt
     9841 agattttcaa tttcatcaga ataacgacag ttttacccta cattttcaac aacgtcttat
     9901 tttaacccat aacaaagata atccttgttt atggattggc tcaggtatag cggatatcga
     9961 tatgttccgc ggtaatttca gcattaaaga taaactacag gagaaaattg cgcttaccga
    10021 cgccatcgtc agccagtcac cggatggttg gttaattcat ttcagccgtg gttctgacat
    10081 tagcgccacg ctgaatatct ctgccgacga tcagggccgt ttattgctgg aactacaaaa
    10141 cgacaacctt aaccacaacc gtatctggct gcgccttgcc gctcaaccag aggaccatat
    10201 ctacggctgc ggcgaacagt tttcctactt cgatctgcgc ggcaaaccgt tcccgctatg
    10261 gaccagtgaa caaggcgttg gtcgcaacaa acaaacctat gtcacctggc aggccgactg
    10321 caaagaaaat gcgggcggcg actattactg gactttcttc ccacagccta cgtttgtcag
    10381 cacgcagaag tattactgcc atgttgataa cagttgctac atgaacttcg actttagcgc
    10441 cccggaatac catgaactgg cgctgtggga agacaaagct acgctacgtt ttgaatgtgc
    10501 tgacacatac atctccctgc tggaaaaatt aactgccctg ctgggacgcc agccagaact
    10561 gcccgactgg atttatgacg gggtaacgct cggcattcag ggcgggacgg aagtgtgcca
    10621 gaagaaactg gacaccatgc gtaacgcggg cgtgaaggtt aacggcatct gggcgcagga
    10681 ctggtctggg atccgtataa cctcttttgg caagcgcgtg atgtggaact ggaagtggaa
    10741 cagcgaaaac tacccgcaac tggattcacg cattaagcag tggtatcagg agggcgtgca
    10801 gttcctggcc tatatcaacc cgtatgttgc cagcgataaa gatctctgcg aagaagcggc
    10861 acaacacggc tatctagcaa aagatgcctc tggcggtgac tatctggtgg agtttggcga
    10921 gttttacggc ggcgttgtcg atctcactaa tccagaagcc tacgcctggt tcaaggaagt
    10981 gatcaaaaag aacatgattg aactcggctg cggcggctgg atggctgact tcggcgagta
    11041 tctgcccacc gacacgtacc tgcataacgg cgtcagtgcc gaaattatgc ataacgcctg
    11101 gcctgcgctg tgggcgaagt gtaactacga agcccttgaa gaaacgggca agctcggcga
    11161 gatcctcttc tttatgcgcg ccggttctac cggtagccag aaatactcca ccatgatgtg
    11221 ggcgggcgac cagaacgtgg actggagtct cgacgatggc ctggcgtccg tagtcccggc
    11281 ggcgctgtcg ctggcaatga ccggacatgg tctgcatcac agcgacattg gcggttacac
    11341 caccctgttt gagatgaagc gcagcaaaga gctgctgctg cgctggtgcg atttcagcgc
    11401 cttcacgccg atgatgcgca cccacgaagg taaccgtcct ggcgacaact ggcagtttga
    11461 cggcgatgcg gaaactatcg cccattttgc ccgtatgact accgttttca ccaccctgaa
    11521 accttatctg aaagaggccg tcgcgctgaa tgcgaagtcc ggtctgccgg tcatgcgccc
    11581 gctgttcctg cattacgaag acgatgcaca tacctattcc ctgaaatatc agtacctgtt
    11641 aggccgcgac attctggtcg ctccggtaca cgaagaaggt cgcagcgact ggacgctcta
    11701 tctgccggag gataactggg tacatgcctg gacgggtgaa gcgttccggg gcggggaagt
    11761 taccgttaat gcgcccatcg gcaagccgcc ggtcttttat cgcgccgata gcgaatgggc
    11821 ggcactgttc gcgtcgttaa acagcatcta atcacatccg cccgtgggga aactcatggg
    11881 caaagggaga acatcgatga gtcacatcac aacggaagat ccagcaactt tacgcctgcc
    11941 ctttaaagag aaactctctt acggtattgg cgacctggcc tctaacatcc tgctggatat
    12001 cggtacgctt tatcttttga agttttatac cgacgttctg gggctgccag gcacctatgg
    12061 cggcattatc tttttgattt caaaattctt tactgcgttt accgatatgg gtaccggcat
    12121 tatgttggat tcccgacgca agatcggtcc aaaaggtaag ttccgtcctt ttattctgta
    12181 tgcgtcattc ccggtcacct tactggcgat cgccaacttt gtcggcacac cgtttgatgt
    12241 caccggtaaa acggtgatgg ccactattct gtttatgctt tacggactgt ttttcagcat
    12301 gatgaactgc tcctacggcg caatggtccc ggcaatcacc aaaaacccca acgaacgcgc
    12361 ctcactggcg gcatggcgtc agggcggcgc tacgctgggc ctgctgctgt gcacggtggg
    12421 attcgtgcca gttatgaatc ttatcgaagg taatcagaaa cttggctata tcttcgccgc
    12481 cacgctgttt tcactgttcg gcctgctgtt tatgtggatc tgctactcag gcgtgaaaga
    12541 gcgttatgtc gaaacccaac caaccaatcc ggcgcaaaag cctggcctgt tgcaatcttt
    12601 ccgcgcaatt gccggtaacc gcccactgtt cattctgtgt attgccaacc tctgcacctt
    12661 aggggcgttt aacgtcaagc tcgccattca ggtctattac acccagtacg tacttaacga
    12721 tcccatcctg ttgtcgtata tgggattttt cagcatgggc tgtattttca tcggcgtgtt
    12781 cctgatgcct ggcgcagtca gacgttttgg taagaagaag gtctatatcg gcggcctgct
    12841 gatttgggtg ctgggcgatc tgctcaacta tttcttcggc ggcggctcgg tcagctttgt
    12901 ggcgttctcc tgcctggcgt tcttcggctc agcgcctgaa ctgggcgctg gtttccgaca
    12961 ccgtcgagta cggcgagtgg cgcaccggcg tgcgttcgga aggcacggtc tacaccggct
    13021 tcaccttctt tcgcaaagtg tctcaggcgc tggctggttt cttccccggc tggatgctga
    13081 cgcaaattgg ctatgtgccg aacgtcgcac aggctgacca caccattgaa gggttacgcc
    13141 agttgatctt catctaccca agcgcactgg cggtagtcac cattgtggcg atgggttgct
    13201 tctacagcct gaacgagaag atgtatgtcc gcattgtgga agagatagaa gcccgtaaac
    13261 gcacggcgta ataataataa acgacgccct gcggggcgtt ataaggagtg attatgtctg
    13321 accataatcc actgacatta aaactgaatc tgcgggaaaa aatcgcctat ggtatgggcg
    13381 acgtcggttc gaatttaatg ctctgcatcg gtactctgta tctcctcaaa ctttataccg
    13441 atgaattagg gatgcctgct tactatggcg ggattatctt tctggtcgcg aagtttttta
    13501 ccgcgtttac cgatatgctc accggatttt tactcgactc gcggaaaaat attgggccta
    13561 aaggcaaatt tcgtcccttt attttatatg cagcagttcc ggcggcgtta attgcaacgc
    13621 tccagtttat cgccaccacc ttttgtttgc cggttaaaac gacgattgcc accacgctgt
    13681 ttatgatgtt tggactttca tacagtctga tgaactgctc gtatggtgcg atgatcccgg
    13741 caattaccaa aaacccgaat gagcgcgcgc agctcgcggc ttaccgccag ggtggtgcaa
    13801 ccatagggct attgatttgt accgtggcgt ttattccgtt gcagtcgctt ttttctgact
    13861 caaccgtcgg ttatgcctgt gcggcactta tgttctccat tggcggcttt atttttatga
    13921 tgctgtgcta cagaggcgtc aaagagcatt atgtggacac agcgccaacc ggacataaag
    13981 ccagtattct caaatctttt tgcgcgatat ttcgtaatcc accattgctg gttttatgca
    14041 ttgctaacct gtgtactctg gcggcattta atatcaaact ggcgattcag gtctattaca
    14101 cccagtatgt gctgaatgat attaatttat tgtcgtggat gggattcttc agtatgggat
    14161 gcatcctcgt cggcgtatta ctggtgccag taactgtaaa atgttttggt aaaaaacagg
    14221 tttatttagc tggcatggtg ctatgggcgg tgggtgatat actgaattat ttctggggaa
    14281 gtaactcttt cactttcgtc atgttctctt gtgtcgcctt ttttggcacg gcctttgtaa
    14341 acagcctgaa ctgggcactg gtaccagata ccgtagatta cggtgaatgg aaaaccggta
    14401 ttcgtgccga aggttctgtt tataccggtt ataccttctt tcgtaaaatt tctgccgcac
    14461 ttgctggctt cttgccaggc attatgctga cgcaaattgg ttatgttccc aacatcgcgc
    14521 aaagcgatgc gacattacag ggtttgcgtc agctcatttt tatctggcct tgtgcactgg
    14581 caattattgc tgcattgacg atgggattct tttacacact caatgaaaaa cggtttgcat
    14641 tgattattga ggaaatcaac caacgcaaaa ataaagagat ggcgacagaa aaaaaaacgg
    14701 cttccgtaac gttataaatg tcaatagccg ctatttccat cctggtgggt ggcggcctcc
    14761 ctacgtttaa aaaatggact tattatgaaa aagattaatg caataatttt attatcttct
    14821 ctgacctctg cctcggtatt tgctggcgca tacgtcgaaa acagggaggc atataatctt
    14881 gcctccgatc agggcgaggt tatgctacgt gtgggttata acttcgatat gggcgcgggt
    14941 attatgttaa ccaataccta cacctttcag cgagaagatg aactaaaaca tggctataat
    15001 gaaattgaag gttggtatcc tttattcaaa ccaaccgata aattaaccat ccagcccggt
    15061 ggcttaatta atgataagag tatcagttcg ggtggtgcag tgtatctgga cg
//
LOCUS       DAETQL010000090        14628 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.090, whole genome shotgun sequence.
ACCESSION   DAETQL010000090 DAETQL010000000
VERSION     DAETQL010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.090"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..183
                     /locus_tag="K0W65_003200"
     CDS             <1..183
                     /locus_tag="K0W65_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3096356.1"
                     /translation="SRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVE
                     LHDKVIGHYLNIKHYQ"
     gene            complement(200..334)
                     /locus_tag="K0W65_003201"
                     /pseudo
     CDS             complement(200..334)
                     /locus_tag="K0W65_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
     gene            517..660
                     /locus_tag="K0W65_003202"
     CDS             517..660
                     /locus_tag="K0W65_003202"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096357.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(879..2417)
                     /gene="aldB"
                     /locus_tag="K0W65_003203"
     CDS             complement(879..2417)
                     /gene="aldB"
                     /locus_tag="K0W65_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HBI3096358.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2582..3733)
                     /gene="yiaY"
                     /locus_tag="K0W65_003204"
     CDS             complement(2582..3733)
                     /gene="yiaY"
                     /locus_tag="K0W65_003204"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HBI3096359.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3923..5767)
                     /gene="selB"
                     /locus_tag="K0W65_003205"
     CDS             complement(3923..5767)
                     /gene="selB"
                     /locus_tag="K0W65_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBI3096360.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5764..7155)
                     /gene="selA"
                     /locus_tag="K0W65_003206"
     CDS             complement(5764..7155)
                     /gene="selA"
                     /locus_tag="K0W65_003206"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBI3096361.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(7253..7861)
                     /locus_tag="K0W65_003207"
     CDS             complement(7253..7861)
                     /locus_tag="K0W65_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBI3096362.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7932..9017)
                     /locus_tag="K0W65_003208"
     CDS             complement(7932..9017)
                     /locus_tag="K0W65_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBI3096363.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(9020..9382)
                     /locus_tag="K0W65_003209"
     CDS             complement(9020..9382)
                     /locus_tag="K0W65_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBI3096364.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9919..11832
                     /gene="mtlA"
                     /locus_tag="K0W65_003210"
     CDS             9919..11832
                     /gene="mtlA"
                     /locus_tag="K0W65_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBI3096365.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11927..13075
                     /gene="mtlD"
                     /locus_tag="K0W65_003211"
     CDS             11927..13075
                     /gene="mtlD"
                     /locus_tag="K0W65_003211"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBI3096366.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            13075..13662
                     /gene="mtlR"
                     /locus_tag="K0W65_003212"
     CDS             13075..13662
                     /gene="mtlR"
                     /locus_tag="K0W65_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HBI3096367.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13674..13883)
                     /locus_tag="K0W65_003213"
     CDS             complement(13674..13883)
                     /locus_tag="K0W65_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096368.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            14167..14529
                     /locus_tag="K0W65_003214"
     CDS             14167..14529
                     /locus_tag="K0W65_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBI3096369.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3588 a         3889 c         3674 g         3477 t
ORIGIN      
        1 tcacgcctga agggaaagct gcacgttatc agcaagcgtt acactcagcg cattgagcga
       61 cataatctga atctgagaca acatctggca aggctgggac ggaagtcact gtcgttctca
      121 aaatcggtgg agctgcatga caaggtcatc gggcattatc tgaacataaa acactatcag
      181 taagttggag tcattaccca tgaatggcgt cggcatgagg atgattacgt tttttagcaa
      241 tcaggtaggg aatatcatgt aaaataataa tcccatagaa tatcaccaga aatacaaaaa
      301 taagcactcc cagtgcaaaa tagtccagga acatatttcc ctcaaagaat ataaaaaaga
      361 acaattaacg catattatga aatgccatga tgcagtgcaa tttccttatt tatataacga
      421 catattgttt tttatatact ttctgaaatg tgctgtctgg tttttcaaca ggacgaggtt
      481 tatcatattg aaatagtgga actattggcc aaactaatga ataactccag taaaacatcc
      541 acagtacaga ttaagcgtat taaaccttca attatctacc gtttattgct gattggcctc
      601 ggatcaccaa tggtgattta cggcctggtt cgcccgctca ccatcgaaac gcgagattaa
      661 actcctgaca aaacacgccc cagaaacgca cttctgttgg cgtagtctgg gttattgcgc
      721 acgtaggttt aaaacgtaaa ttgtaacaac gtgcgctttg tttatgccgg atgcggcgtg
      781 tacgccttat ccggcctacg gtcaggttcc cgtaggcatg ataagacgcg taagcgtcgc
      841 atcaggcaat gaatacccaa tgcgaccagc ttcttatatc agaacagccc caacggttta
      901 tccgagtagc tcaccagcag gcacttggtt tgctggtaat gctccagcat catcttgtgg
      961 gtttcgcgac cgatacctga ttgtttgtag ccaccaaacg cagcatgtgc cgggtaagcg
     1021 tgataacagt tggtccacac gcgcccagcc tgtatgccgc gccccatctt ataggccaga
     1081 ttaccgttgc ggctccagac gcccgcgccc aggccatatt gcgtatcgtt cgccagctcc
     1141 agcgcttctt ccatcgtttt gaaggtggtc accgccagca ccgggccaaa aatctcctcc
     1201 tggaacaccc gcatattgtt ctgaccaaac agaatcgtcg gttcgaggta gtagccgtct
     1261 ttcagttcac cttccagcag cttgcgccgc ccgcctgtga gcacgtcagc gccctctttt
     1321 ttaccgatat caatgtagtt gaggatggtt tccagttgcc cgtgagaaac ctgcgcgccc
     1381 atttgcgtca cgctgtcgag cgggttaccg ctgcgaatgc tttcgacacg gcggatggcg
     1441 cgttccataa agcgttcgta gattgactcc tgcactaagg cacgactcgg acaggtgcaa
     1501 acttcgccct ggttaaaggc aaacagtgca aagccttcca gcgctttatc gaaaaaggca
     1561 tcttcttcat ccatcacatc agcaaagaag atatttggcg acttaccgcc caactccagc
     1621 gtcaccggaa taatgttttg cgttgcgtat tgcataattt gttggcccac ttccgttgag
     1681 ccggtaaacg ccactttggc gatgcgtttc gaggtcgcca gatattcgcc aatttcccca
     1741 cctgcgccat tgaccacgtt caccacgccc ggcggcagta aatcaccgac aatttccatt
     1801 agcagcagta cagaaagcgg ggtaagacgt gcgggtttca gcaccacaca gttgcccgcc
     1861 gccagcgcgg gagccatttt ccagctcgcc atcagcagcg ggaagttcca cgggataatc
     1921 tgccccacca cgcctaacgg ttcatggaaa tgataggcca cggtttcgct atcaacttca
     1981 ctgatcccac cttcctgcgc ccgaatacac gaggcgaaat agcggaaatg gtcaatcgcc
     2041 agcggtacat ccgcagcact ggtttcgcga atgggtttgc cgttatccca ggtttcagct
     2101 gtcgctaaca gctcgaggtt ttgttccatt cgatcggcaa tcttaaacag aatcgccgcg
     2161 cgatcctgca ccgaggtgtg cgcccattta tctttcactt tgtgcgcagc atccagcgcc
     2221 agatcgatgt ctcgtttgcc cgaagacgcc acttcgcaca gcagctgccc ggtcaccggc
     2281 gtcagattct ggtaatactc accatcggca ggggctaccc attcaccgcc aataaagttg
     2341 tcgtagcggg cttttaactt gatggggaaa ccatactcgc cgggcttaat ctgtgctgaa
     2401 gggggattat tggtcatgat cgtctccttg cggtgtgagg tataacaagg gtagacgtga
     2461 ctggcgaaat cttcgccagt cggtaacagc tttacgacag ctatcacgaa tttacgggca
     2521 aggattacag agatagttga aaaagcggct gacaatttgc cagccgttgt tggaatgatg
     2581 attacattgc ggcgcgataa atcaccacaa tttcttcgtg agttgcctgg atcgggttag
     2641 taaagccaca ggcatctttc agggcattag tcgccagaac cgcgaaatct tcttctttca
     2701 cgttcaggtc gcgtaggcct gccgggatat ccactttctt cgccagttca cggatggcgt
     2761 taatgcaggc ttcagcacct tccgcgtcgt ttttacctgt cacgttcacg cccattgcag
     2821 cggcacagtc acgcagacgt gcagcggcga ctttgctgtt gaatacctga acatgcggca
     2881 gcagaacggc gttacataca ccgtgtggca ggttgtagaa accgcccagc tggtgcgcca
     2941 tcgcatgaac ataacccaga gaagcattat tgaacgccat accggcaagg aactgggcat
     3001 aagccattgc ttcacgcgct ttcgcattac tgccatcttc aacggctaac ggcaggtttt
     3061 cggcaatcat ggtcacggct ttcagtgcac aagcgtcagt gatcggcgtg gcggcaatag
     3121 aaacatacgc ttcgatagcg tgcgttaagg catccatacc cgttgcggcg gtcagtgact
     3181 tcggcatacc aatcatcaga gaggagtcat tgacagaaag cagcggagtc acgtgcttat
     3241 caacaatcgc cattttgtta tgacgcgctt cgtcagtgat gatgcagaaa cgggtcattt
     3301 cagaggccgt acccgccgtg gtattgatgg cgatcatcgg cagctgcggt tttgcagagc
     3361 ggtcaacgcc ttcgtaatcg cgaatatcgc cgccattggc tgccaccagc gcaatacctt
     3421 ttgcgcagtc gtgtggagaa ccaccgccta aggagatcac gctatcgcaa ttattttctt
     3481 taagtaattt caaacctgcg gcgacgtttt ccgtggtggg gttaggttgg gtgccatcat
     3541 aaataacgct aaaaatattg cgttcttcca gtgctttttg cacatcaccc gccataccta
     3601 atttcgttaa catattgtca gtgacaatta aggtacgggt aaatccataa tctgccatca
     3661 tattcattgc atcagtcaat gaatcagcgc cgatgacatt cacagaagga ataaagaaag
     3721 ttgaagatgc cataacacac tcctgaaagt gtaaaaaagg gtgggcagct tactgaggat
     3781 tttcattcga aaacatgatc attattatta attatcaaaa ttaatctaat aaaaagcaca
     3841 ttgtattcat taaacaatgt gctttattta aaatcataag aaatttgtat taatttattt
     3901 taaaaattaa ttaatcattt ccttattttt ccggaaataa taatgcgtcg cgcaataaat
     3961 gatcatttcc acgacgacgc gtaaagccaa tgcggtcaaa atattccaga atctgaattg
     4021 ccagctttcg gcctacgcct aagcgatcgc ggaaatccgc cgcgcaggtt gaaccacact
     4081 cctgatcgag atcgcggatc atattggcaa attcgacaat ccgatcgtta cggtaataac
     4141 gatctttgac gatcgcggta attattcctt gctgcgccgc ctggcgtagc gtcaggcgca
     4201 ttgcctgctc gtcggtaccc gtctcttttg ccagatcacg cacccaccac ggctcgtcac
     4261 caaacagcgg ctctgctttt tgccagatgg cctgctgctc ttcgctgaag cccgctttgt
     4321 gatccggcag atgcagccaa ccgtgatgac tgaggatgtc gccgctttcg cgcatctttt
     4381 caatcagcaa cagtactagc gcttcatctt ccattggcaa cgccatacgt cgcagacgtt
     4441 cgcgcccagg cccaggttca tcgcgatgtt gctcatgata agtcgctaat gtgtcgagaa
     4501 ttttccgttg ccagcgagcg gcaaccggcg cattcaacaa gctataacca gcctgaatat
     4561 agccaggctg ttgcagcagt tcgcgcatcc cttcgccgtt gagctggcgc gcccaagcga
     4621 aatcaggaag attaaccgcg ccgcgttgca ggtgaacggc taacgcttcg ctatcattct
     4681 gtgccctggc gagggaagcc agccattgca gatactctgg tttacgttta ccgcgacgcg
     4741 gcgggttaag catcacgacg cgcgctccgg ccagcgtgtt gcgggcagag atatcgcgca
     4801 ataccaggcg gtcgttatct gccagccata acggggtgtc gaagaccagt tcagcaaggt
     4861 tatcttccag cagtgaaacg cgtcccgtga cgtggctggc ggcgtggtga atatgcagcg
     4921 gctgccactg ggtcagcggt gcatgggttt gaagctcgac aatcacccgt gtgaacggct
     4981 ctggcggcac atcggcaagc agccagtcgc cacggttaat ctgctctttt tccgcatcac
     5041 ccgcgatgtc aagcgcgata cgctgcccgg cattggcggt ttctgttggc tggttttgcg
     5101 catgcagcgc acgtacacgc atcggtttat ttacaccagt cagccagagt gaatcgccta
     5161 ccttcacttc cccgcttaac gccgtaccgg tgacgaccag cccggcacct tttacggtaa
     5221 atgcgcggtc aatcgcgagg cggaaactat gttggctggc gtgctcgcgt tccggcaact
     5281 gaagcagatg ctcgcgcagg gcatcaattc cccgaccttc ggttgctgcg gtgataaaca
     5341 gttttgcctc agcaaaaccg tattcccgca gaacctcctt tacctggcgt tcaacctcat
     5401 caacacgcgc ttcgtccacg cgatcggctt tggtcagcgc cactgtcagc atcgggttac
     5461 cggtcagctg caaaatcgcc agatgctcac gggtctgtgc catcacgccg tcatcgcacg
     5521 ccaccaccaa cagcgcgtga tcgataccac caacgcccgc cagcatgttg gaaagaaact
     5581 tttcatgacc gggaacgtcg ataaaaccag gcacgcgacc atccggctgc ggccagtagg
     5641 catagccgag atcgatggtc atgccgcgct ttttttcttc cggcagacgg tcagcattta
     5701 cgccagtaat cgcctgcaat aaggttgttt tgccgtggtc aacgtgtccg gcagtcgcaa
     5761 taatcatttc aacaacatct ccaaaaaccg ttgctcatct tcaaggcagc gtaaatccag
     5821 ccacaatcgt ccgtcataaa tacgaccaat caccggcact ggcaattcac gccagcgggc
     5881 ggctaatgac tcaaggtggc taccgcgtcc atcatggggt gtaaacgtta atgccgcgct
     5941 cggcaggcga tcaaccggca gcgaaccact gccaatctgc gaaagacatg gcataacctg
     6001 taccgcaaac tccgcgccgt aatgtgcgac aaggggagcc tgtaaacgtt gtgcctggat
     6061 ttgaatgacc tctgcgctgc gggtaagcag gcgcagggtc ggtaattttt cactcagagc
     6121 ttcagggtgt aaataaagac gcaacgtggc ttccagcgcc gcgagggtca ttttatccgc
     6181 gcgtaatgca cgcttcagcg ggtggctttg caggcgggcg atcatctctt ttttaccaac
     6241 gataatcccg gcctgcggcc cgcctaacaa cttgtcgccg gagaagctca ccagactgac
     6301 gcccgccgca atcaactcct gcggcattgg ctctttcggc aaaccgtact ggctaagatc
     6361 gaccagcgag ccactgccta aatcagtcac cactggaaca tccagctctt tgccgagcgc
     6421 caccagttca gcttcgtcta tcgctttggt gaacccctga atgctgtagt tactggtatg
     6481 tactttcatc aacagtgcgg tattttcatt caccgcctga cgataatcat tcgcgtgcgt
     6541 gcggttggtg gtccctactt cgtgtagggt gcagcctgcc tgacgcataa catcgggaat
     6601 acgaaacgcg ccgccaatct ccaccagttc gccgcgagat accaccacct ctttcccgct
     6661 ggcagtggcc gccaacatca ataacaccgc cgccgcattg ttattgacga tacaggcatc
     6721 ttccgccccc gtaatacggc acagcagctg cgccagcgcc cgatcgcgat gtccgcgtcc
     6781 ggcgtcgtcc agatcgtact cgagggtcac tggcgaacgc atagcctgcg caacggcttc
     6841 caccgcggct tccgcctgta aagctcgccc aaggttggta tgcagcacgg ttcccgtcag
     6901 gttgatcacc ggacgcagcg cgctctgcgc ctctttcgtc aaccgggcat cgacttcttg
     6961 cgcccagttt tcacaccacg caggcagcgt ctggctgcca cgaatcactt ctcgcgcttc
     7021 gtcgagcatc tgacgcaaca attccaccac gcgggtgtga ccataagtat cacgcaaaga
     7081 aaggaaggag ctatcgcgca ataagcgatc aatagccgga agttgactat agagggaacg
     7141 cgtttcggtt gtcataggaa acctggctga tcaaggccct ctcacacgga gaagggcgtt
     7201 taacataacc acggattgta acgtgagatg ggtcaggagg acatatcgcg catcaagcct
     7261 ttggcggttc ggtgcgagca aaactttcgc ggctaaacag gttttcgacc agtttgacta
     7321 agtgtgggcg gtcgacacac caacctggcg caacgcggcg gaaattgaga tatccgacag
     7381 cacaggcaat ggcgatagtc gccagattga ccgtatcggt tttgagtgtg ccatcgacca
     7441 gatatccttc cagcacatcc agactgcggt tgattttctc ccgctggcgt aacaattcat
     7501 cttcagactg ctgcgccgcc ggacgcgcct gttcacgcac cgataccagc ccggcatcca
     7561 taatgccatc cgccagcgcc tcaattttgc gcacccgcaa cgactccagc ggatcgcgcg
     7621 gcaacatcgc cggagcgaca ttcattaact caatatattc agcgatgatc ggcgaatcaa
     7681 accagcattc gccctcttcg gtcaccagca ccggcacttt ccctaacggg ttaaattgcg
     7741 ccacgccgtt atccgcgtta tagggcagtt cattaataaa ttcgaaagtt atgccctttt
     7801 ctaacaacag aataaaaagt ttgcgtacaa acgggctggt gtagctaccg acgagtttca
     7861 tgccgagtcc tttgtgcgag gaaaaatatc agtatggctc aggctcacgc aaaacacagg
     7921 tgatataagt ctcaatgatc caaataaaga taatgcatcc aactggtcat tcttagcagc
     7981 actttccgca tcaccgaaac atggctgaaa tggtcggagt aaaccgccac ctgggcgtag
     8041 atgccgtcgg gtaaggcatc gatatcatcg ttagggtcca gttcaatggt tcccagcaca
     8101 ccgtccgtgc cgggaacgac cgttaatgat tgcaataccc cctgcgcctg ataagaaccg
     8161 cctggcacga caggtaaaat actagtcaat ttgccgtgga acacctgccc aggtagcgcg
     8221 ttaaacacca cttccgcatc atcgccaggt ttcagacgta acagcgagtt ttgccgaaat
     8281 tgggcgacaa tttgccgttt ttgctcgggg atgaagacca tcaccggacg cagcggcaag
     8341 gcagctgcgt atgtacctgg gcggatcagt acctgagtga cgtagccatt gcttggtgcg
     8401 cgaatgacag tctgctcaag attatatttt gcttcagtaa gttgcgctct taagctcaca
     8461 atctgcgatt gctcgccgtt gaccatactg tcgagctgac tctggatctg cgcctgctcc
     8521 gccaccgagc ctttcactaa cgcatcctgc gcgaggaaat tttgccgcgc atcgtcgatg
     8581 tcacgttccg agaacggatt caccgccgcc tggctgcctt tcaggtaacg ttgataattt
     8641 ttaaacagac ggtcgcgctc cgctgaaacc tgggtggtgt tggcctgcgc ttcagtgagc
     8701 tgcgcacgca gcgtctttat attatgcgtc gccgtcatca ggtcagcctg aagtctgtca
     8761 actcgcgcct ggtaacgaac cgggtcgagc ttaaaaagca cctcgccctt ttgaataagc
     8821 tgattattct tgtcagtgac ttcagtaaca attcccgtca cctgtggcgt gatagggatc
     8881 gctatcactg ccttttgcgc ggtaaaagtg taagggtggt tgtagttcat caacaaaatc
     8941 aaaccactca ccagaaacac gcctcccagc gccgccgtcg ccagcgccac gtaagttaaa
     9001 acaatcaata gatccataat tagatctccg ggaacgtgga atagtcagtc tttttctcgg
     9061 cggcgagttg atgctccagc cgggaaattc ggtcagaaag tgcggcgatt tccggttcag
     9121 tcgctttctc ctgcgacgca acatgtgact gcatacccca gccacgctcc ggttgataga
     9181 gcgtcgccca gatccacaga aacggccaga taacatgcag agtaaacagg ctcacccagc
     9241 ccgccgtatg aatagcgtcg gcatgggggt gattgcgctt tttggcaatc agataaggga
     9301 tgtcatgtat cgcgatgatt ccataaaaaa tcaccagaaa gacgaagatc agcactccca
     9361 acgcgaaata gtttagaaac atatctgcct cggattcacg tttatcagtg ttgttttttg
     9421 gctggcagcc agaagggagt caggctgata ttttgacaat aatccgggtt cgcgattctc
     9481 gccataacac caaagaataa tttttagagg tgatgagttg cttagttaca taacgattgt
     9541 atgacgaagg cataacatgc tgtagatcac atcaggtgaa cgccgtaaga aaatatcttg
     9601 tgattcagat cacaaagatt caacaaacca tcaaaacaaa aatgtgacat tactcacatt
     9661 taaatgccat ttttagcgaa aatcaccgcc ttgtttcttt tttatacagg cgttttgtga
     9721 tgagcgtcac gtcaattacc tctctacccc ctatatttat gtgactgata tcacacaaaa
     9781 gcccgtcgac tggacagtta accgattcag tgccagattt cgcagtatct acaaggtccg
     9841 gctacctctg ccgccacatt aacaaaaaac ctcgggcttc cagcctgcgc gacagcaaac
     9901 ataagaaggg gtgtttttat gtcatccgat attaagatca aagtgcaaag ctttggtcgt
     9961 ttcctcagca acatggtgat gccaaatatc ggcgcgttta tcgcgtgggg tatcatcacc
    10021 gcgttattta ttccaacagg gtggttaccg aacgagacgc tggcgaagct ggtcgggccg
    10081 atgatcactt atctcctacc gctgctgatc ggttataccg gtggtaagct ggtaggcggc
    10141 aaacgtggtg gcgtagtcgg tgccattacc accatgggcg ttatcgtcgg cgcagacatg
    10201 ccgatgttcc tcggttctat gattgcaggt ccgctgggcg gctggtgcat taagcacttc
    10261 gaccgctggg tagacggtaa gatcaaatcc ggttttgaga tgctggtgaa taacttctcc
    10321 gcaggcatca tcgggatgat cctcgctatt ctggcattcc tcggcattgg cccgattgtt
    10381 gaagccctgt ccaaaatgct ggctgcgggc gttaacttca tggttgtcca tgacatgctg
    10441 ccgctggcgt ctatctttgt tgaaccggcg aaaatcctgt tcctcaacaa cgccattaac
    10501 cacggtatct tctcgccgct gggtattcag cagtcccatg aactgggtaa atcaatcttc
    10561 ttcctgattg aagctaaccc aggtccaggt atgggcgtgc tgctggcgta catgttcttt
    10621 ggtcgtggta gcgctaaaca gtctgcgggc ggtgcggcaa tcatccactt cctgggtggt
    10681 atccacgaaa tctacttccc gtatgtgctg atgaatccgc gtctgatcct cgcagtcatc
    10741 ctcggcggta tgactggcgt gttcacgctg actatcctgg gcggtggtct ggtttctccg
    10801 gcatctccgg gttctatcct tgctgtactg gcgatgacac caaaaggtgc ttatttcgct
    10861 aacatcgcgg gtgtgtgtgc ggcgatggct gtctccttcg ttgtctctgc tattttgctg
    10921 aaaaccagca aagtgaaaga agaagatgat attgaagcag caactcgtcg tatgcaggac
    10981 atgaaagctg agtctaaagg cacatctccg ctatctgcgg gcgatgtgac taacgacctg
    11041 agccacgtac gtaaaatcat cgttgcctgt gacgccggta tgggttcaag tgcgatgggc
    11101 gcaggcgtgc tgcgtaagaa aattcaggat gcaggtctgt cgcagatttc ggtcactaac
    11161 agcgcgatca acaacctgcc gccagatgtg gacctcgtca tcactcaccg tgacctgacc
    11221 gaacgcgcta tgcgccaggt tccgcaggca cagcatattt cgctgaccaa cttcctcgac
    11281 agcggcctgt acaccagcct aaccgaacgt ctcgttgctg cccagcgcca tactgaaaac
    11341 gaagtgaaag tgaaagacag tctgaaagac agctttgacg attccagtgc taacctgttc
    11401 aaactgggcg cggagaacat cttcctcggt cgcaaagcag caaccaaaga agaagcgatt
    11461 cgttttgctg gcgagcagct ggtgaaaggc ggttacgttg agccggaata cgttcaggcg
    11521 atgctggatc gtgaaaaact gaccccgact tatctgggtg agtctatcgc ggtgccacac
    11581 ggtacggttg aagcgaaaga tcgcgtactg aaaacgggcg tggtgttctg ccagtacccg
    11641 gaaggcgtgc gcttcggtga agaagaagat gacattgccc gtctggtgat tggtattgca
    11701 gcccgtaaca acgagcacat tcaggttatc accagcctga ccaatgcact ggatgatgag
    11761 tccgtcatcg agcgtctggc acacaccacc agcgtggatg aagtgctgga actgctggca
    11821 ggtcgtaagt aatccaatcc taccctctcc tcacggagag ggtttgggtg agggaaaagc
    11881 ctcaccccag ccctctcggg taaaaacatt gatgaaggtt aatactatga aagcattaca
    11941 ttttggcgca ggtaatatcg gtcgtggctt tatcggtaaa ctgctggcag acgcgggtat
    12001 ccaactgacg tttgccgatg tcaatcaggt ggtacttgat gccctgaatg cccgtcatag
    12061 ctatcaggtt catgtggtcg gtgaaaccga gcaggtagat accgtttccg gcgtcaatgc
    12121 tgtcagcagc attggtgatg atgtcgttga tctgattgct caggtcgatt tagtcactac
    12181 cgccgttggc ccggttgtgc tggaacgtat tgctccggct atcgccaaag ggctggtgaa
    12241 acgcaaagaa caaggtaatg aatccccgct gaacatcatc gcctgtgaaa acatggtacg
    12301 cggcaccacg cagctgaaag gtcatgtgat gaacgccctg ccggaagacg ccaaagcgtg
    12361 ggtagaagag cacgttggct ttgtcgattc cgccgttgac cgcatcgtac cgccttcggc
    12421 ttcggcaact aacgatccgc tggaagtgac ggtagaaact ttcagcgaat ggattgtcga
    12481 taaaacgcag ttcaaaggcg cactgccgaa catcccaggc atggagttaa ccgacaacct
    12541 gatggcattt gtcgaacgta aactcttcac cctgaacacg ggtcatgcta taaccgcgta
    12601 cctcggaaaa ctggccggtc atcagaccat tcgtgacgct attctcgacg agaaaatccg
    12661 cgcggtggta aaaggtgcga tggaagaaag tggtgcggta ctgatcaagc gctacggctt
    12721 tgacgcagac aaacatgcgg cgtacatcca gaaaatcctc ggtcgttttg agaacccgta
    12781 tctgaaagat gatgtagagc gcgtaggccg tcagccgctg cgtaaactga gtgctggcga
    12841 ccgtctgatc aagccactgc tcggtacgct ggaatacagc cttccgcaca agaatctgat
    12901 tcaggggatt gctggtgcaa tgcacttccg cagtgaagat gatccgcagg ctcaggaact
    12961 ggcagcactg atcgctgaca aaggtccgca ggcggcgctg gcacagattt ccggtcttga
    13021 tgccaacagc gaggttgtat ccgaggcggt aaccgcttat aaagcaatgc aataatggtg
    13081 gaccaggcgc aggacaccct gcgcccgaat aacagattgt cagatatgca ggcaacaatg
    13141 gaacaaaccc aggccgttga aaaccgtgtg cttgagcgtc tgaatgctgg caaaaccgtg
    13201 cgaagctttc tgatcaccgc cgtcgagctt ttgaccgagg cggtaaatct tctggtgctt
    13261 caggtattcc gcaaagacga ttacgcggtg aagtatgctg tagaaccgtt actcgacggc
    13321 gacggtccgc tgggcgatct ttctgtgcgt ttaaaactca tttacgggtt gggcgtcatt
    13381 aaccgccagg aatacgaaga tgcggaactg ctgatggcat tgcgtgaaga gctaaatcac
    13441 gacggcaacg agtacgcctt taccgacgac gaaatccttg gaccctttgg tgaactgcac
    13501 tgcgtggcgg cgttaccacc gccgccacag tttgaaccag cagactccag tttgtatgca
    13561 atgcaaattc agcgctatca acaggctgtg cgatcaacaa tggtcctttc actgactgag
    13621 ctgatttcca aaatcagctt aaaaaaagcc tttcaaaagt aagcaacgtc tgcttactgc
    13681 ccctctacct gcttcggccg ataaagccga cgataatact ccagacgttg tagatataac
    13741 ggcacgcttt catcgggtat tccggacgga atcgcgttac ggggaggaag ttttttcaga
    13801 tactcccgga acgcctggct tgatgccatg aaatctacgg ctttatcgat aagaagcgga
    13861 acgttttcac ctaatttgcc catgatgata tctccgtatt accccgcgcc gggaatgcgc
    13921 ggccgccaat tttagtttag gttcccggta aagatgacat taaggaaaac gtgctgaatc
    13981 ctcaataatc atccgctgct gtttattatc tttttctata gatttcttaa ttaatcaacg
    14041 aattgcgtag tttttgagca tgttaatgat tacgttgcag aactattaca atgtcatcag
    14101 ttgtgaacaa agcacctggt cgcgcatact aggggctata aatttatctt tatcagaagc
    14161 catcacatga aagaagtcga aaaaaacgaa atcaagcgtc tcagcgatcg cctggacgcc
    14221 atccgccacc agcaggccga tctgtcgctg gttgaagccg cagacaaata tgccgagctg
    14281 gaaaaagaga aagccacgct ggaagcagaa attgctcgcc tgcgtgaggt tcatagccaa
    14341 aaactgagta aagaagcaca gaagctgatg aagatgccgt tccagcgcgc aattaccaaa
    14401 aaagagcagg ctgatatggg caagctgaag aaaagtgttc gcggactggt ggttgtgcac
    14461 ccaatgaccg cgctggggcg cgaaatgggc ctggaagaga tgactggatt ttctaagact
    14521 acgttttaag cccctcagac gactggttcg atcgtatcaa cccgcatctg gcttgcctta
    14581 gctggtcaat gcggggactc tcctgtctac tcgcctaatt accaaaca
//
LOCUS       DAETQL010000091        14482 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.091, whole genome shotgun sequence.
ACCESSION   DAETQL010000091 DAETQL010000000
VERSION     DAETQL010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14482
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.091"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(116..382)
                     /locus_tag="K0W65_003215"
     CDS             complement(116..382)
                     /locus_tag="K0W65_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBI3096370.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            656..4180
                     /gene="nifJ"
                     /locus_tag="K0W65_003216"
     CDS             656..4180
                     /gene="nifJ"
                     /locus_tag="K0W65_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBI3096371.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4547..5676
                     /gene="ompN"
                     /locus_tag="K0W65_003217"
                     /pseudo
     CDS             4547..5676
                     /gene="ompN"
                     /locus_tag="K0W65_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5817..6251
                     /gene="uspF"
                     /locus_tag="K0W65_003218"
     CDS             5817..6251
                     /gene="uspF"
                     /locus_tag="K0W65_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBI3096372.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6427..7362
                     /gene="ttcA"
                     /locus_tag="K0W65_003219"
     CDS             6427..7362
                     /gene="ttcA"
                     /locus_tag="K0W65_003219"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBI3096373.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7491..8858)
                     /gene="dbpA"
                     /locus_tag="K0W65_003220"
     CDS             complement(7491..8858)
                     /gene="dbpA"
                     /locus_tag="K0W65_003220"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBI3096374.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9336..10319)
                     /gene="zntB"
                     /locus_tag="K0W65_003221"
     CDS             complement(9336..10319)
                     /gene="zntB"
                     /locus_tag="K0W65_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBI3096375.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10574..11806
                     /gene="dgcM"
                     /locus_tag="K0W65_003222"
                     /pseudo
     CDS             10574..11806
                     /gene="dgcM"
                     /locus_tag="K0W65_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11827..12390)
                     /gene="smrA"
                     /locus_tag="K0W65_003223"
                     /pseudo
     CDS             complement(11827..12390)
                     /gene="smrA"
                     /locus_tag="K0W65_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12644..14098)
                     /locus_tag="K0W65_003224"
     CDS             complement(12644..14098)
                     /locus_tag="K0W65_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HBI3096376.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14095..>14482)
                     /locus_tag="K0W65_003225"
     CDS             complement(14095..>14482)
                     /locus_tag="K0W65_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBI3096377.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3862 a         3651 c         3418 g         3551 t
ORIGIN      
        1 ataacaccat tagcgaaatg ctgaaagaag gtgcacaagt ggatctgacc gcgaaccagt
       61 taacgctggc gaccgcgaaa cagacattaa cttataagct ggcggattta atgaattaat
      121 agctgccaca gctcccggcg gcaagtgact gttcgctaca gcgtttgccg ttgggtaatg
      181 cacacatccc aatcgccgta ccatccagtt gacgggcaac agaaagcgaa ccgccgatca
      241 ttgcacaatt tgcttctcca ctactggaca tcgacgcttt taaacttggc gctacgtgcg
      301 ccgcagtcgc ctgctgaaca ggctcactac tacacgccga caacaataaa gcggcacacc
      361 ctacccaaaa cgctgctcgc atctcttctt cctctgatct tcaagccaaa cgacaccgcc
      421 ataaataata ggcagcacag agggcggcgt cgagagctgt cctgcgcgtt gccccgcatt
      481 tttacttttt tatggctatt tttttgccct ctgtttgatc aaaacattca ttactctgat
      541 gtgggggaca caaaagcgaa aatgcagaag aaagccattt gctaaaattg aaggattact
      601 actgggcgcg cagcaatttc gtgcgcccct catttgcgca atgtaagggt gtcatatgat
      661 tactattgac ggtaatggcg cggttgcttc ggtgacgttt cgcatcagtg aagttatcgc
      721 catctaccct attaccccca gttccacgat ggcagaacag gctgatgcct gggccggaaa
      781 cggcttgaag aacgtttggg gagacacacc acgcgtggtt gaaatgcagt cggaagcggg
      841 tgctatcgct accgtgcatg gcgctttgca gacgggtgcc ctttcaacat cgtttacgtc
      901 atcgcagggt ttgctgctga tgatcccgac gctgtacaaa ctggcaggcg aactgacgcc
      961 atttgtcctg catgtagcgg cacgtaccgt tgccacacat gcactttcta tttttggcga
     1021 tcattccgac gttatggcgg tgcgccagac gggttgcgcg atgttgtgtg cggctaacgt
     1081 ccaggaagcg caagactttg ctctcatttc gcacatcgca acgctgaaga gccgcgtgcc
     1141 atttattcat ttctttgatg gttttcgcac gtcccacgaa atcaataaaa ttgtcccgct
     1201 ggccgatgac actattcttg atctcatgcc gcaggccgaa attgatgctc atcgcgcccg
     1261 ggcgctcaac ccggaacatc cggtgatccg cggtacgtcc gccaatcctg acacttattt
     1321 ccagtctcgc gaagccacca atccctggta caacgcggtc tatgaccatg ttgaacaggc
     1381 gatgaatgat ttcgctgccg cgacaggtcg tcagtatcag ccgtttgagt attacgggca
     1441 tccgcaagcg gaacgggtga ttatcctgat gggctctgcc attggcacct gtgaagaagt
     1501 ggttgatgaa ttgctaaccc gtggcgaaaa agttggcgtg ctgaaagttc gtctgtaccg
     1561 tcccttctcc gccaaacact tgctgcaagc tctgccggga tccgtacgca gcgtggcggt
     1621 actggacaga accaaagaac ccggtgccca ggcagaaccg ctctatctgg atgtgatgac
     1681 cgcactggca gaagccttta ataatggtga gcgcgaaacc ctgccccgtg tcattggtgg
     1741 gcgctatggt ctttcatcca aagaatttgg cccggactgt gtactggcgg tatttgccga
     1801 gctcaacgcg gctaaaccta aagcacgctt tacggttggt atttacgatg atgtgaccaa
     1861 tctgtcactg ccgttgccgg aaaacactct gccaaactcg gcgaaacttg aagccttgtt
     1921 ttatggcctt ggtagtgatg gcagcgtttc cgcaaccaaa aacaatatca agattatcgg
     1981 taattccacg ccgtggtacg cacagggcta ttttgtttac gactccaaaa aagcgggcgg
     2041 cctgacggtt tctcaccttc gcgtgagcga acagccgatt cgttccgctt atctcatttc
     2101 ccaggctgat tttgttggct gccaccagtt gcagtttatc gataaatatc agatggctga
     2161 gcgtttaaaa cctagcggca ttttcctgct taatacgccg tacagcgcag atgaagtgtg
     2221 gtcgcgcttg ccgcaggaag ttcaggccgt gttaaaccag aaaaaagcgc gcttctatgt
     2281 gattaacgcg gcgaaaatcg cccgcgaatg tggcctggcg gcccgtatta ataccgtcat
     2341 gcagatggct tttttccatc tgacgcaaat tctgcctggc gatagcgccc tcgcagaatt
     2401 gcagggtgcg attgccaaaa gttacagtag caaaggtcag gatctggtgg agcgcaactg
     2461 gcaggctctg gcgctggcgc gtgaatccgt agaagaagtt ccgttgcaac cggtaaatcc
     2521 gcacagcgcc aatcgaccgc cagtggtttc cgatgccgcc cctgatttcg tgaaaaccgt
     2581 aaccgcggcg atgctcgccg ggcttggtga tgccctcccc gtttcggcgc tgccgccaga
     2641 cggcacctgg ccgatgggca ccactcgctg ggaaaaacgc aatatcgccg aagagatccc
     2701 catctggaaa gaggaactct gtacccaatg taaccactgc gtcgccgctt gcccacactc
     2761 agctattcgc gcaaaagtgg taccgcctga agcgatggaa aacgcccctg ccagcctgca
     2821 ttcgctagat gtgaaatcgc gtgatatgcg cgggcagaaa tatgtcttgc aggtggcacc
     2881 ggaagattgc accggttgta acctgtgcgt cgaagtttgc ccggcgaaag accgtcagaa
     2941 tccagagatt aaagccatca atatgatgtc tcgccaggaa catgtcgaag aagagaaaat
     3001 caattacgat ttcttcctca acctgccaga aatcgaccgt agcaaactgg aacgtattga
     3061 tattcgtaca tcgcagctga ttacaccgct gtttgaatat tcaggtgctt gccccggttg
     3121 tggcgagacg ccgtatatta aattgctgac tcagctctat ggtgaccgga tgttgatcgc
     3181 taacgccacc ggctgttctt caatttatgg cggtaacctg ccctctacac cgtataccac
     3241 cgatgccaac ggtcgtgggc cggcatgggc gaactctcta tttgaagata atgccgaatt
     3301 tggccttggt ttccgcctga cggtcgatca acaccgtgtc cgcgtgctgc gtctgctgga
     3361 tcaatttgcc gataaaatcc cggcggaatt actgacggcg ttgaaatcag acgctacgcc
     3421 agaggttcgt cgagaacagg tcgcagcttt acgccagcaa ctcaacgatg ttgccgaagc
     3481 acatgaactt ctgcgtgacg cagatgcact ggtggaaaaa tcaatctggc tgattggtgg
     3541 cgacggctgg gcttacgata tcggctttgg cggtctggat catgtattga gtttgacgga
     3601 aaacgtcaac attctggtgc tggatacgca atgctattcc aacaccggtg gtcaggcgtc
     3661 gaaagcgaca ccgctgggtg cagtaactaa gttcggcgag cacggcaaac gtaaagcgcg
     3721 taaagatctt ggcgtcagta tgatgatgta cggtcatgtt tatgtggcgc agatttctct
     3781 cggcgcgcag ctgaaccaga cggtgaaagc gattcaggaa gcggaagcgt atccgggacc
     3841 atcgctgatc attgcttata gcccgtgtga agagcatggt tacgatctgg ctctcagcca
     3901 cgaccagatg cgccaactca ctgctaccgg cttctggccg ctatatcgct ttgatccgcg
     3961 tcgtgccgat gaaggcaaac tgccgctggc gctggattca cgcccgccgt cagaagcact
     4021 ggaagaaacg ttacttcacg agcagcgttt ccgtcgtctg aattcgcagc agccagaagt
     4081 ggcagaacag ttatggaaag atgctgcagc taatttgcaa aaacgctatg acttcctagc
     4141 acaaatggcc ggaaaagcgg aaaaaagcaa caccgattaa atgctctgga taaggattat
     4201 ccaattctaa aaaaaaagcc cggacgactg ttcgggcttg tctttttata tgttggaaaa
     4261 tcagtggcaa tgcaatggcc caacagaaaa taaaatctgt gacaataaag gcatataacc
     4321 cgcgcagaat aacgtatagg aaattaaaag tataattttt attttgtatg tattatcttc
     4381 gatacattca ccagacttat tatcatttca gaggaattat ttgattaagg ttttacttaa
     4441 ggcgtaacaa atgatattta tcggctaact gaacttctcc tttaggatgt tttcactccc
     4501 cgaaatgggg ataaaaggca aataaaataa ctaaaggatt tattcaatga aaagaaaagt
     4561 actggcactt ttaattcctg ccctgctcgc cgcaggtgct gcacatgcag ccgaagttta
     4621 taataaagac ggcaacaaat tagatctgta tggcaaagtt gatggcctgc attatttttc
     4681 tgataattca gcgaaagatg gcgaccagag ctatgcgcgt ctgggtttta aaggcgaaac
     4741 ccaaattaac gatcaactta ctggctacgg tcaatgggaa tacaatattc aggcaaacaa
     4801 cactgaatct tcaaaaaacc agtcatggac ccgtctggca tttgccggac tgaaatttgc
     4861 agattacggt tctttcgatt acggacgtaa ttatggcgta atgtacgaca tcgaaggctg
     4921 gaccgatatg ctgcctgaat ttggcggtga ctcttatacc aatgcagaca actttatgac
     4981 tggtcgagcc aatggcgtcg cgacttatcg taatactgat ttcttcggtc tggtaaatgg
     5041 tctgaacttc gcggtgcagt atcaaggtaa caacgaagga gccagtaatg gtcaggaagg
     5101 caccaataac gaacgcgatg ttcgtcatga aaacggtgac ggctggggtc tttccacaac
     5161 atatgattta ggcatgggct ttagcgctgg tgcggcatac acctcttctg accgcaccaa
     5221 tgaccaggtt aaccatactg cggcgggtgg tgataaagca gacgcgtgga ctgctgggct
     5281 aaaatacgat gctaacaata tttacctagc aaccatgtat tcagaaacgc gtaatatgac
     5341 cccgtttggc gacagcgatt atgcagttgc gaacaaaacc cagaactttg aagttactag
     5401 taccagtttg atttttgtct gcgtccggca gtctctttcc tgatgtctaa aggccgtgac
     5461 cttcacgctg ctggtggtgc agacaacccg gcaggtgttg atgataaaga tctggttaaa
     5521 tacgccgatg ttggcgcgac ttactatttc aataaaaaca tgtccaccta tgttgactat
     5581 aaaatcaacc tgttggatga agatgacagt ttctacgctg ccaacggcat ctctaccgat
     5641 gatattgtcg ctttaggtct ggtttatcag ttctaaatcc tcctgcccgc tgttatggcg
     5701 ggctttttct gcttattctt cctcctttga tctaaattaa aaatgtgaac tccgtcatta
     5761 cacaaaaagt gtcatctggc gttacacttt atgcggatac taaaacagga ggttttatga
     5821 acagaacgat tcttgtccct atcgatattt cagattcaga attaactcaa cgcgtgatta
     5881 gccacgttga ggcagaggca aagattgatg atgcagaggt tcatttcctg acggtaatac
     5941 cttcactgcc ctactatgcc tctctgggtt tagcgtattc cgcagaatta ccggcaatgg
     6001 atgacctgaa agcggaagcc aaatcgcaac tggaagagat cattaaaaaa tttaaactgc
     6061 caaccgacag agtgcatgtc catgttgagg aaggctcgcc caaagaccgc attctggaat
     6121 tggcgaagaa gatccccgct catatgatca tcattgcttc ccatcgaccg gatatcacca
     6181 cttatctgct cggttccaac gccgcagctg tagtgcgtca cgcagagtgc tccgtgctgg
     6241 ttgtgcgctg acactaacgc ccgcacattg ctgcgggctt tttgattcat ttcgcaaatg
     6301 tgctgacatt ttcccctcta atccgtacca tacacgccac agtttttata tcagatttct
     6361 tatgctgagc gttccggcat gacgcatact cttctgatgc catataacga attgagtcgc
     6421 ttttaaatgt cgcaaaatca agaaattagt aagaaagaac aatacaacct gaacaaatta
     6481 caaaaacgtc tgcgtcgtaa cgtgggcgaa gccattgctg acttcaatat gattgaagaa
     6541 ggcgatcgca tcatggtttg cctctccggg ggtaaagaca gctataccat gctggagatt
     6601 ctgcgcaatt tgcagcaaag cgcgccaatc aatttttcgc tggtggctgt taacctcgat
     6661 caaaagcaac cgggcttccc ggaacacgtt ctgcccgagt atcttgaaaa gctgggcgtt
     6721 gagtacaaga ttgttgaaga gaacacttac ggtatcgtga aagagaagat tccagagggc
     6781 aaaaccactt gctcactgtg ttctcggcta cgtcgcggta tcctttatcg caccgcaacg
     6841 gaactgggga cgacgaagat cgcgttgggt caccatcgtg acgatatcct gcaaacgttg
     6901 ttcttaaata tgttctacgg cggcaagatg aaaggtatgc ctccgaaact gatgagcgat
     6961 gatggcaaac atatcgttat tcgtccactg gcctactgcc gcgagaaaga tattcagcga
     7021 tttgccgatg caaaagcgtt cccgattatt ccgtgcaacc tgtgcggttc acagcctaac
     7081 ctgcaacgtc aggtgattgc tgacatgttg cgtgactggg ataaacgtta tccagggcgt
     7141 atcgagacga tgttcagcgc gatgcagaat gtggtgccgt cgcatctgtg cgataccaac
     7201 ctgttcgatt tcaaaggcat tacccacggt tctgaagtgg ttaacggggg tgatctggcg
     7261 tttgatcgcg aagagatccc actacaaccg gcgggctggc agccagaaga agatgaaaat
     7321 cagttggatg agttacggct gaatgtggtt gaagtgaaat aaccaggata gcgcccgatg
     7381 cgcaagcgta tcgggctact cttatggagg ccggataaga cgcggccagc gtcgcatctg
     7441 gcaatcccga ataagatgtt tactcttgca cccggcaatt caacatttca ttattttaat
     7501 aaccgcaccc ggcacgtttt tcctttaatc ttcccgccct gtaactgttt ccatgcttta
     7561 tgagcaacag cctgacggac cgcgacatag acatgcgccg gatgcacggc gattttgcca
     7621 atatctgcgc catcaagccc gatatctcct gtcagtgcac ctaatacatc acccgggcgc
     7681 attttggctt ttttcccgcc atcgatacac aacgttgcca tttctgcttc cagcgtcaca
     7741 atggaactat tagctggcgg cgtttgccag ttaagtttta tctgcaacat gtcagaaatg
     7801 atattggccc gctgtgcttc ttccggagca cagaaactga tcgccagacc gctatttcct
     7861 gcacgagctg tacgaccgat gcgatgtaca tgaacttcag ggtcccacgc cagctcaaag
     7921 ttcaccacca gctcaagcga tttaatatcc agaccacgcg cagcaacatc agtcgcaacc
     7981 agtacacggg cgctaccgtt agcaaaacgt accagggtct gatcgcgttg ctccagatcg
     8041 ccgtgtaacg acaatgcact ttgctctact tcattcagcg cgtcgcagac agcctggcaa
     8101 tcttttttgg tattgcaaaa caccacacaa gaggatggct gatgcaagct taataaccgt
     8161 tgcaacagag gaattttgcc tttactggat gtctcataaa attgttgttc aatgggtggc
     8221 aaagcatctg ttgagtcaat ttcaatcgcc aaaggatcgc gttgcactcg tccgctgatt
     8281 gcggcgatgg cttccggcca ggttgccgaa aacagaagcg tctgtcgaga tgcaggcgca
     8341 aaacggatga catcatcaat ggcatcgcta aatcccatat ccagcatgcg gtcggcctca
     8401 tccatcacca aagtattcag cgcatccagt gataccgtgc ctttttgcaa gtgatccagc
     8461 aaacgacccg gcgttgccac gataatatgc ggcgcatgtt gcagcgaatc acgctgcata
     8521 ccgaacggtt gaccaccgca caacgtcaaa attttggtat ttggcagaaa acgcgccagc
     8581 cgacgcaatt cacctgccac ctgatccgcc agttcacgcg taggacacag cactaaagcc
     8641 tgggtttgaa atagcgacgc atcaatttgc tgtaacaagc cgaggccaaa agccgccgtt
     8701 ttgccgctgc cggttttcgc ctgcacgcga acatcttttc cggcaaggat cgccggaagc
     8761 gcggcggcct gcaccggcgt catggttaaa taacccaact cattaaggtt cgtgagttgg
     8821 gcgggaggca aaacattcag ggtagaaaaa gcggtcacaa tctattctcg tggtcatcga
     8881 cgcaaagtta gcaggcgcgt atcctcgcag atctacgctc acgatgcgac aatttaatcg
     8941 gttcttcatc gggtggtggg tcaggcatgg gttgcgggcg agggatcgga tcgggcactg
     9001 gaacaggatc gccaggaatc ggttcaggga caggaatttg caaataaata agtgtcgtca
     9061 tatttccctc cggtcattgg gtggactctt aaagggtaga cgctgataaa taacaggcaa
     9121 aaaaaagccg actcatcaaa gtcggcgtcg tacgaatcaa ttgtgctatg cagtaattca
     9181 aaaaaggaag taagacaata tggagcgcaa cgcccatcgc ttgacgttgc attcacctgc
     9241 aagggagata ttgccctgaa tgggtagaga gtttattgac ttcgctcaaa ctttgcggcg
     9301 tttttgtata cagacagccg gaaaaattgc ttttgttaca accatttact acggtgcaac
     9361 cataaagcaa caccaccaat aagaacaact aacagaatac aaaaaattga aaatccgaat
     9421 tgccacccgc cgccagggat cccaccaagg ttgacgccaa ataacccggt cagaaaggta
     9481 ctgggtaaaa agaccattgc catcaacgac attgtatagg tacgacgagc taaattttcc
     9541 tgcatcacct gagcgatttc atccgccatc acgccagtcc gtgctataca ggcgtcgatt
     9601 tcgtcaaggc cgcgcccaag gcgatcggca atatcctgca tccgacggcg ttggtcatcg
     9661 ctcatccacg gcaaacgttc actggcaaga cgagcataaa catcacgttg cggtgccata
     9721 tagcgacgca tcacaattaa ttgtttgcgc agcagagcca ggaatccacg cggtggaatt
     9781 tgctgatcaa ggagattatc ttcaaggtcg ataattttat cgtgcagctg ctcgataaat
     9841 tcactggaat gatcggtcaa cgcatcgcac acatccacca gccatccccc gcaatcggtc
     9901 ggacccgtgc cctcttccag atcgctcacc acatcgtcca gcgccagcac tttgcgttgt
     9961 cgggtcgaaa caattaaccg cccgtccata tatacacgca tggcgaccag ttgatcgggg
    10021 cgttcatcgg tgctgccgtt tatacagcgc aatgtaatca gcgtgccttc accgaaacgg
    10081 ctgactcggg gccgtgtgct ctcgcccgcc agcgcatcac gtacgttatt gggaagcagc
    10141 ggtgttgtcg ccagccattg ggcgctatca tggtgtacat aattaaggtg gagccagcag
    10201 ggatgcgctt catcaatcac atctgtattt tccagcggtt taacgccgcc tctaccatcc
    10261 agcatccagg caaatactgc atccgggaca ttaacgtccg atcccttaat cgcttccact
    10321 gtgcctccat catcaacgca ttattttgta gtctagcctt ctggccctgt tacgcaacat
    10381 ctcatcaccc cattaccctg aaatgattaa taaaattctg tctaaattga atacaaaaag
    10441 caaaatgctt ttccgtatac aaaccgtgtg aagtgttaaa tagcgtctat cattatcaga
    10501 attatctgat catatgacgt ggcttttttg cgatcggata gcaacaaaaa ttgataaaaa
    10561 taacgggatc tcaatgatta cgcacaactt caataccctg gacttactca ccagtcctgt
    10621 ctggattgtt tcgccctttg aggaacagtt aatttatgcc aatagcgcgg cgcgactgtt
    10681 gatgcaagac ctcacgttta gtcagctacg aaccggaccc tattccgtct cctcacaaaa
    10741 agaactgccg aaatacctct ccgatctgca aaaccaacac gatattatcg aaatcctcac
    10801 tgttcagcgt aaagaagagg aaacagcatt aagctgtcgg cttgttttgc gagagctgac
    10861 agaaacagaa ccggtgatta ttttcgaagg tatcgaagcg ccggcaacgc tgggtttaaa
    10921 agccagtcgc tcggcaaatt atcagcgcaa aaaacaaggt ttttatgcgc gcttttttct
    10981 gactaactct gcaccaatgt tgttgattga cccgtcacga gatggacaaa tcgtcgatgc
    11041 taacctcgcc gcgctcaatt tctatggtta taaccatgaa acgatgtgcc agaaacatac
    11101 ctaggaaata aatatgcttg ggcgtcgcgt catgcctatc atgcatgaaa tttcgcattt
    11161 acccggcggt cataaccctt tgaattttgt tcataaactg gcggatggat ctactcgtca
    11221 tgtgcagacc tatgccggac cgattgaaat ttatggcgac aagctcatgt tatgtattgt
    11281 gcatgatatt actgagcaaa aacggctgga ggagcagctg gaacatgctg ctcaccatga
    11341 cgcgatgacc ggattactga atcggcgaca gttttatcac attacggaac caggccaaat
    11401 gcagcatctc gccatcgctc aggattacag cttgttgctc atcgacaccg atcgttttaa
    11461 acacattaac gatctctatg ggcattctaa aggtgatgag gtgttatgcg ccctcgcccg
    11521 ccccctcgaa agttgcgctc gcaaaggcga tttggtgttt cgttggggag gcgaagagtt
    11581 tgtcttattg ctaccaagaa ccccactgga taccgcgctt tcgctggctg aaactatccg
    11641 cgtaagcgtg gcaaaagtga gtatttcggg cttaccacgc tttaccgtca gcattggtgt
    11701 ggcgcatcac gaaggaaatg aaagcatcga tgaactgttt aaacgcgttg atgatgcttt
    11761 gtatcgggcg aaaaatgatg gacgcaaccg cgtgctggcg gcataagccg cggatgcgtc
    11821 tcgagatcaa cgactgcgct tagcgtggcg ctcccagttt tcttgcttcg cctgcgccgt
    11881 tttacgtagt gcgacgtaac acgccccgct gccgccatga tgcggtagcg cggtgcaata
    11941 tgcctgaaca tcatcaaatt cggtcagcca gcgcgccaca tagctgcgga caatattggc
    12001 atgtgattta tcttcccgcc ctttaccatg aataatcagc acattacgca aaccatccgc
    12061 cagggcttgt tgaatgaaac tgaacaccat tttgcggcac tcttccaccg gctggcgcaa
    12121 aagattcagg ctcgcctgtt gcggatattt accactgcgc agcttatcca gcaccccatg
    12181 ctacaaccct tcctgccgaa actccagcgg ctgacttagc gggatgatgt cgagaaatcc
    12241 ggtagtgagg aaattatcaa gctgcagcgt gtcgatacgc tgcggcgcac gttggttacg
    12301 cgttggatgc cagtggacat ctgtagcacg tttcagcggc tggacatctt ccatggcgtc
    12361 aagaaacagc gatttgtcgt caaggttcat gtaacctcct atgcaccttc acacgtcgtg
    12421 attcagacgg gttaattgtt catacttact gcatcgttcc cttcacagat tgaaacaata
    12481 ctctacttca tacctgtgag tatctgcgca ggcggggcat tttatactag cctgttagca
    12541 gtatcgaaca gtttagcccg agtttaattc agcacaggct ccgatcagga tgatttattc
    12601 atagcaggaa ccaacaaaaa tgcgataaat aacagaccac tgattagcag aaaggtaccg
    12661 aaaatgccat agtttgcgcc tccctcagca tcaattgact caataatttg gttgaagcca
    12721 gtatttccga tgtctggtca cttctactgc gattaaaaaa taaagcactt ctggaaatga
    12781 agtcattcac cgtatgggga acagaaacca ttttttcacc cattctcgaa aaatgaaaat
    12841 tagttcgatt attgagaata gagccacaca gggcaatacc aatcgcgccc ccaagattac
    12901 gtgttaaatt aaatacacca gaggctaact tcagccgttc ttttggaatt ccccccagcg
    12961 ttaatgttac gatgggggcc atcgcaaact gctgcgaaat ccctctaatt gcctgtggaa
    13021 acagtaattc ctgccatccc cattcgtggg ttataggggt aaaaagatac attgaaaaca
    13081 caaatccgcc cagccccgcc atcaacagcc atcgcagatt tattttttta cttaaccaga
    13141 agtaaaaggg aacagaaaat aactggaaaa tacctgttgt gcaaacggca aaacctatct
    13201 cttcggcatt taatccgcga atctgcccca aaaacacagg tattaaatat acagtagaga
    13261 atatcccaac ccctccagaa aaagaaaaaa aacatcccag agtgaaattt ttatccttaa
    13321 aagcatgaag atccattatc ggattggata ttgtaagtgt acgcgcagca aataaaataa
    13381 aactcaccaa tgccagaact gatgtcagca aaatagtatt atcatccagc catccccagc
    13441 gcgctccttc ttcaagggta tattccagac atcccagagt catggccaac aggataatac
    13501 tcggataatc agcaactttc agtaaagaga gatcaggttt atcaaaatta accagaaaag
    13561 gaatactcaa aactaaatat atgccaggta atatattgat ataaaatagc catcgccagt
    13621 cgaggttatc agtgagccag ccccccagag ttggccctag cgtcggtgac agtgaggcca
    13681 gcgcactgac taccgcagca gccaacccca actctttacc ctggtaataa ataaacgcca
    13741 tcgtgaaaac taaagggatc atggatgcgc cagcagctcc ctgtaaagca cgaaacaaaa
    13801 tcatgatctg aatattccat gcaagtccac acgcgatact cattaaagta aatattcccg
    13861 cggatagagt aaaaagccaa cgagtggaaa atacccgtga cagccaccca gagagcggaa
    13921 taacaataat ctcagcaatt aaatagctgg tctgaagcca ggccatttcg tctttacctg
    13981 cggacagacc accacctatt tcatttaggg atgacgatac aatttgaata tcaagctgag
    14041 caataaataa tcccatacac atactggcga aagcaatatg tttacgcata gacatcatct
    14101 ctcatcaaca ctgacggtga cggacagtcc cgggcgaaga agttgcttta attcctttgc
    14161 gtcgtcaaat gcaatccgta ccgggacacg ctgaacaatt ttggtgaaat tgccagtggc
    14221 attttctatc ggaatcaagc tgaaactggc tcctgtcgct ggggaaaggc tttcaatgtg
    14281 accgtgaaac acttcccctt tgagaatatc agcacggatc tcagccttca ttcctggctt
    14341 cattcccgct atttggtttt ctttataatt tgcatcaacc cacaactcac tgactggtac
    14401 tagtgaaact aacgatgtgc cgccttccac ccaactgcct gtatgggcgc tgcggtttgc
    14461 gacaatgcca tcaatcggcg ca
//
LOCUS       DAETQL010000092        14346 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.092, whole genome shotgun sequence.
ACCESSION   DAETQL010000092 DAETQL010000000
VERSION     DAETQL010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.092"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..3051
                     /locus_tag="K0W65_003226"
                     /pseudo
     CDS             1..3051
                     /locus_tag="K0W65_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3061..6707
                     /locus_tag="K0W65_003227"
                     /pseudo
     CDS             3061..6707
                     /locus_tag="K0W65_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6768..7175
                     /locus_tag="K0W65_003228"
     CDS             6768..7175
                     /locus_tag="K0W65_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096378.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7717..8805
                     /locus_tag="K0W65_003229"
     CDS             7717..8805
                     /locus_tag="K0W65_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBI3096379.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8816..11095
                     /locus_tag="K0W65_003230"
     CDS             8816..11095
                     /locus_tag="K0W65_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096380.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11088..12225
                     /locus_tag="K0W65_003231"
                     /pseudo
     CDS             11088..12225
                     /locus_tag="K0W65_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            12222..14252
                     /locus_tag="K0W65_003232"
     CDS             12222..14252
                     /locus_tag="K0W65_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096381.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASVDAFHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVG
                     SGIAGEAKLDHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSP
                     AATRRLFSFQAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPL
                     RQPGIVALREYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEG
                     EDNLLTLSLPASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKT
                     RAWALDAETAPVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQA
                     ELLANDLTAVGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3550 a         3769 c         3662 g         3365 t
ORIGIN      
        1 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
       61 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      121 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      181 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      241 atctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      301 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      361 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      421 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
      481 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
      541 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag
      601 tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag
      661 cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat
      721 gccagcacca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccatag
      781 tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
      841 cgccattagg catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
      901 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
      961 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     1021 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     1081 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggagg
     1141 ctggagaaag cagtaaaagc atggaatgcc cttactctat ctccacatac cgataccgaa
     1201 tacgccatgt tacattttgg actcaaaggt ttacctggtt tagttaactc aattacacgc
     1261 tatccacagg aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc
     1321 gtcgcccgtg ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac ctggctgttg
     1381 aaatacccgg aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa
     1441 gcacaggata acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta
     1501 ctgcaagaaa tcgcccggcg ttataaccag tcggaagtaa cagacgcggt gaacgctctg
     1561 cttgcgctcg atcccttaga taatcacccg acaaaaatcc ccactcttcc gaccttttat
     1621 cagccatcga tctggacgcg cccggtatta aaagcaaacg cccaatcact gccagataac
     1681 gccctcctcc acctcggcga aatgctccgc ttccctcagg aagaggctct gtatccggga
     1741 ttattgcagg tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc ctgggatctg
     1801 tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta
     1861 ggcgttctcg gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct
     1921 ggtgaatccc agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt
     1981 gatatcgccc tgatgcagct taacggcatt gctcagaaac tgaaattcaa agcattacag
     2041 gagcgggcaa aagaaaagat tgccgacatt gccgagagcc gcgaactcac ggtagcggag
     2101 cttgaagatc ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat
     2161 tttggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat
     2221 gtttccggca gccgcctgaa agacctgcca aaaccaaaca aaagcgatta tgagtcacag
     2281 gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgcggcc
     2341 cagcaggtag caaggcttga atccgccatg tgcctgcgtc gccgctggtc gccggaaaac
     2401 ttccaactct tcctggttgt gcatccgctg gttcgccact taacccgccg tctgatttgg
     2461 ggcgtttata gcgccgacaa ccagctactg gcctgtttcc gggtagcgga agataacagc
     2521 tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcatt
     2581 cctcacgttc tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac
     2641 tacgaactgc taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc
     2701 gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc
     2761 atggggctgg caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatgatc
     2821 aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtata
     2881 tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
     2941 gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
     3001 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
     3061 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
     3121 ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa
     3181 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt
     3241 gtctggcgtt atgaaaattt ttcaccggag tggcagtccg ccattacgca ggccatcaac
     3301 ttgatcggcg aacacaaacc ctcagtcccg gcccgcacaa tggcggcact agcctttatc
     3361 gcgcaaaatg acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat
     3421 gcgactgagg tggtgattgc acgccagttt attgcacggt gttatgagag tgatcctctg
     3481 gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc agaaacctat
     3541 aacgaattcg atctccgact gcgtaaacac ctctctctgg cagaggaaag ctgctggcag
     3601 cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt
     3661 attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag ccttgaatgc
     3721 ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctactga ccccaaagcg
     3781 gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga agccagctac
     3841 atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga
     3901 ctggcagtcg ttccgcgcct cgcgatatat gcccataaag aagattgcgg cagtctgctg
     3961 gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa
     4021 cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac actcgccgca
     4081 ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc aatcatcgaa
     4141 gacaaaaagc tgcctgcaca gcaaaaagct cgagatgaat actggcgtac gctgttacaa
     4201 acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt aagtactcaa
     4261 gcccgggcag tgctgaatag ttatttatcg gcaccgccca aaccagttat tgatagcacc
     4321 gataacagcc aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag aaagaaaaag
     4381 acttccatac gtcacttttc gctgcctgat cttacgcttg caccgcaaac gtacaggtcg
     4441 ccgaataaac actggacgcc ggaacagcaa actgcaattg accattttag cgccatgtct
     4501 tttgacgaac gtcttgctac gagaggagcg gatatcttct tgcgggaatt aggttttgac
     4561 gttcatagcc tggaatttaa taagcgtctt cttaatggac agttggatgc ctttgatgct
     4621 cagcaactgc aagaatacac atcaatagga ctattgcgta cttatcacgc gcatcaggcc
     4681 aaatctaaac agtaccggga agacgccacc aatgcattac tggcacaaga tagcattgcg
     4741 ttgatgggag ggtggcgaac ttttcagcac ggattttact atggtaatga acagcgttgg
     4801 gggatttgga atctgtacct tatcgcgcag atgccgcgcg aaatggcggt atcgtgctgg
     4861 caacaaattg tcgaggctga ttttcattac accggggtgg aatatttgtt aagtgtgctg
     4921 ggtaccgatg ctttgcctgg attaaacacc gcctttgccc gacatccaaa agagatattt
     4981 ccgctgctga ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg cgtctggcac
     5041 ctttttgcgg gccagcgtaa tctggctcgc cagtggattt tacaatggcc ggaacatacg
     5101 gctactgcac ttatccctct tgtcttcacc aaatctagcg ataaaagcga agctgcatta
     5161 cttgccctgc gtttacttta cgaacatggg catggcgaat ggctacaaac tgtagccaat
     5221 cgttggcagc gtacagatct atggcctgcc ctggaacaat tacttaaaca gagtccaata
     5281 gagatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccgca aatgtggccc
     5341 aggccgcgcc ttatcactaa taatcaacct gttaccgatg acgctctgga aattatcggc
     5401 gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg
     5461 ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa
     5521 gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa
     5581 agcacggcac gggatctaac gacacatatc ctcgcctggc cacaagaagg caaatctgcc
     5641 cgtgcggtca gtggcatgaa catccttacc ctgatgaata atgatatggc gctgatgcag
     5701 ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc ggcggaattt
     5761 cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc
     5821 ccaacactgg gccttaatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt
     5881 accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag
     5941 aaaagtgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc
     6001 cgactaaaag ggttaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt
     6061 gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt
     6121 aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttat ccggcaaatg
     6181 aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc
     6241 aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa
     6301 tgacagcaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc
     6361 cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca
     6421 gcctgactcg ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca
     6481 aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctgg
     6541 tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct
     6601 tccgttctct tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt
     6661 ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaac aaagcgggca
     6721 gtgatgcgac tgcccgttat ctacacgact taccagcggg gaaagcgatg atcgtgcaaa
     6781 aagagctggt tgctatttac gattatgagg tccctgtacc tgaagatccg ttttccttca
     6841 gacttgagat ccataaatgc tctgaattat ttacaggttc cgtctaccga ctggaacggt
     6901 tccggctacg tccaacattt catcaacgtg atcgagagga tgttgacccg ctaataaatg
     6961 atgcgttgat ttatataaga gatgagttta ttgatgagca aaaattacga ggcgaatcac
     7021 ctgaacctgt aatagtaatt tttaattgta aactacagaa tatattcaac caacaaatag
     7081 aattatatac tctagataat tcaagtcgca gcacatgcaa cttgaagtat gacgagtata
     7141 gctttattaa aggggggaac ggtattcccc cttagatgcg tttcaatttg cccgttactt
     7201 caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt
     7261 taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcacaac accagcacct
     7321 tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg ttgcactccg
     7381 gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa taggttcgat
     7441 ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg caacactgtc
     7501 cagaggtggt tataattcgt tcgttggaac acgatgttgc acaggcagtg gtgttaggcc
     7561 tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta ctgtgatctg
     7621 ctttccagat atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
     7681 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
     7741 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
     7801 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
     7861 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
     7921 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
     7981 aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc
     8041 cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt
     8101 atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
     8161 gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt
     8221 ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt
     8281 cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
     8341 gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
     8401 atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
     8461 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
     8521 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
     8581 accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat
     8641 cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac
     8701 gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa
     8761 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
     8821 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc
     8881 gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggccctgctg attttaatga
     8941 ccgggtagac gaactgtttt tagcccacca gcttccggta gctatttaca gttattgcca
     9001 gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc
     9061 ggagtggcag gcgctacaag ccgatcgtcg tattcaggca caaacttact tcatcgattt
     9121 gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca
     9181 gaccttgctg ctgcgtgcca cccgcatgga taacagcgac accctgtggg atcacttgtt
     9241 cgaagatgaa agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact
     9301 gcggggcgac tcccccggcg atgctctaaa tcgtcagcgc gaagccttta tggcccgctg
     9361 gattggatgg gcgatgcagc aaaataatgg cgacgtgtta gttgtctgcg gtggctggca
     9421 cgctccggca ctggcaaaaa tgtggcgcga atgtccgcag gtaattaaca cgccagaatt
     9481 gccgtcgctg gcagatgccg ttacaggttg ttatctcacc ccctacagtg aaaagcgcct
     9541 tgatgtgctg gcaggatacc tttcaggaat gcctgccccg gtctggcaaa actggtgctg
     9601 gcagtggggc ttacagcagg cgggagaaca actgctaaaa acggttctca cccgtttgcg
     9661 ccagcacaag ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc
     9721 actggcacag ttgcgcggtc atacactacc gttacgcact gactggctgg atgccatagc
     9781 aggatcgctg attaaagaag ccctgaatgc gccgttgccg tggagctatc gcggcgttat
     9841 tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt
     9901 cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca cttgcgaact
     9961 ggaacgtacc gcaatctctc ttccggcgga gcttacctta aatcgcttta accccaatgg
    10021 actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg
    10081 ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg
    10141 cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactcctgga
    10201 agccgcacgc cataaattag aagccgatat gctggacgcg ggtggaatcg gcagcattac
    10261 cacatgtctt agccaagcgg cgtcagcggg tctggcgtcc ttcagtcaac aattactgga
    10321 gcaactcaca ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga
    10381 agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggcg cgcagatatt
    10441 acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ccaacggcag
    10501 accggatgaa aaggagtttc acgctcactt gcatagctgg caagcgttgt gtcatatcct
    10561 gcgcgatcta catagcggtg tgcaactccc cggcgtttcg ctttctgcgg cggtagcctt
    10621 gctggagcga cacagtcagg cgattcatgt cctggcactg gatcgcggcg cgactcttgg
    10681 tgcgctgatg cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc
    10741 gcagttatcc ccggcacaat ccggcgaggc gctgcacggt ttgctggcat tagcccgtca
    10801 tcaactggcc tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaactaag
    10861 tgatgccgat tttaccaatg ccctgcccga tttacgcgcg gcaatggcct ggctaccgcc
    10921 acgagaacgc gggacgctgg cgcatcaggt gcttgagcat tatcaactgg cgcaacttcc
    10981 cgtttcggca ctgcaaatgc cgttgcattg tccaccgcaa gccattgcac atcatcaaca
    11041 actcgaacag caggcactgg catcgctgca acactgggga gttttccatg tctgaactga
    11101 acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag
    11161 aaacgacgct ttgtgggctg gatgacaacg cccggcagat agatcacgcg ctggagtggc
    11221 tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctctagtgga ttaggtggct
    11281 caaatctcac cactcctgag tggatcaaca gtattcacac gctgtttccg caacaggtga
    11341 ttgagcggct ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacaaatc
    11401 tcgacgtgct ggaacgtatg cagccttccg aaagcctgct acgcgccgtt ttgcacacca
    11461 aacatctgat gaaccccgaa gtactggctg ccgcccgcag gattgtgcgc caggttgtgg
    11521 aagaaattat ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc
    11581 gtcgtcgccg ctcatttatt ccactggcgc gaaactttga tttcaaaagt actctgcgcg
    11641 ccaacctgca acactggcac ccgcaacacg gcaagttgta tatcgaattc ccccgcttta
    11701 acagccgcat taaacgccaa agcgagcaat ggcaactggt cttactggtt gatcaaagcg
    11761 gatcgatggt cgattcggtg atccactctg cggtgatggc ggcctgttta tggcagttac
    11821 ccggcattcg tacccatctg gtggcgtttg accacaagcg tcgttgatct cacggcagac
    11881 gttgccgatc cggtagagtt attaatgaaa gtgcaattag gcggcggaac caatatcgcc
    11941 agtgccatgg agtatggtcg gcaacttatt gaacaacctg caaaaagcgt cattatcctc
    12001 gtgagcgatt tttatgaagg gggttcatca tcattgctga cgcaccaagt gaaaaagtgt
    12061 gtccagagcg gcatcaaagt gctgggactg gcagcgctcg atagcacagc aacaccttgc
    12121 tatgaccgcg atacggccca ggcgctggtt aatgtcggcg cacaaatagc cgccatgaca
    12181 ccgggcgagc tggcatcatg gcttgcggag aatcttcagt catgaattca ctacgtccgg
    12241 aattattaga actgacaccg caggcactga cggcgttaag caatgccggt tttgttaagc
    12301 gcagccttaa ggaactggaa aatggcaacg tcccggaaat cagccatgag aacagcgctt
    12361 taatcgccac cttcagtgac ggtgtccgta cccagctggc gaacggccag gcactgaaag
    12421 aggctcagtg cacctatggg gccagcggca tgtgccgtca tcgcgtgatg ctggtgttaa
    12481 gttatcaacg actttgtgct accgcccagc ctacgggaaa agaagaggaa tgggatccgg
    12541 caatctggct ggaagaactg gctacccttc ccgatgccac ccgcaaacgc gcacaggcgc
    12601 tggtcgctaa aagcatcacc attgagttgt tctgtgcgcc gggtgaaatt ccctctgccc
    12661 gcttaccgat gagcgatgtg cgtttttatt cccgcagcag tattcgtttc gcccgttgtg
    12721 attgtattga aggcacactt tgcgaacatg tcgtactggc agtacaggcc tttgtccagg
    12781 caaaagcgca gcaagcggaa ttgactcatt taatctggca gatgcgcagc gaacacgtca
    12841 catcatctga cgatccgttt gccagcgaag aaggcaaaac gtgtcgtcaa tatgttcaac
    12901 aactaagcca ggcattatgg ctgagcggca tcagccagcc gcttattcac tacgaggccg
    12961 ctttcagccg cgcgcagcaa gcagcggaac gctgcagctg gcgatgggtg agtgaatcgc
    13021 tacggcaact gcgcgccagc gttgatgcct tccacgcccg cgccagcgtt gatgccttcc
    13081 acgcccgcgc cagccactat catgccggag aatgcttacg tcagcttgcg gcattaaaca
    13141 gtcgattaaa ttgcgcacaa gagatggccc ggagcgacag tgttggtgaa gttcctcctg
    13201 tgccgtggcg cacggtcgtt ggctctggca ttgccggaga agcaaagctt gatcatctgc
    13261 ggctggtgtc tttaggtatg cgttgctggc aggatattga gcattatggt ttacgcatct
    13321 ggtttaccga tcccgacacc ggcagtattt tgcacctttc gcgcagttgg ccgcgaagtg
    13381 aacaggaaga ctcaccggca gctacgcgtc ggctgtttag ttttcaggct ggcgcactgg
    13441 cgggtgggca aattgtttca caagcagcaa aacgcagtgc cgatggcgag ctgctgttag
    13501 ctacccgcaa ccgcttaagc agcgttgtac cgctgtcgcc tgatgcctgg caaatgttga
    13561 gcgcgccgtt acgccagccg ggcattgtgg ctttgcggga atatttacgc cagcgtcccc
    13621 ccgcctgcat acggcctctt aatcaggtcg ataacttatt tattctgccg gtcgctgagt
    13681 gtatttcgct cggttgggat agcagccgcc agacgctgga tgcgcaggta atcagcggcg
    13741 aaggtgaaga taatctgctg acgttatcac tgccagcctc agccagcgcg ccttatgcca
    13801 ttgaacgcat ggcggcgctt ttgcaacaaa cagacgaccc cgtgtgtctg gtttctggct
    13861 ttgtcagttt tgttgatggg caattgacac tggaaccaca ggtaatgatg acaaagactc
    13921 gtgcctgggc gctggacgca gaaactgcgc ctgtggtggt atcactacct tctgccagcg
    13981 ttctgcctgt gccgtctact gctcatcagt tgctgatgcg ctgccaggcg ttacttattc
    14041 aactgctcca taacggctgg cgctatcagg aacagagcgc tattaatcag gcagagttgc
    14101 tggcgaatga cctcaccgcg gtgggttttt atcggctggc acatgtgttg gcacaatttc
    14161 gtaatacaga aagcgaggca cgggtagaag caatgaataa cggtgttttg ctttgcgaac
    14221 aattattccc catgcttcag caacaaggat gaaatagtgc tttttactaa gagttctact
    14281 ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc ctggtgagag
    14341 tcacca
//
LOCUS       DAETQL010000093        14138 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.093, whole genome shotgun sequence.
ACCESSION   DAETQL010000093 DAETQL010000000
VERSION     DAETQL010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14138
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.093"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..157
                     /locus_tag="K0W65_003233"
     CDS             <1..157
                     /locus_tag="K0W65_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBI3096382.1"
                     /translation="SPGRIPELMRDLASMGQLVKLPTRRGRAFPRVVKERPWKYPTAP
                     KKSQSVA"
     gene            365..2212
                     /gene="ldtD"
                     /locus_tag="K0W65_003234"
     CDS             365..2212
                     /gene="ldtD"
                     /locus_tag="K0W65_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBI3096383.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2393..2941
                     /locus_tag="K0W65_003235"
     CDS             2393..2941
                     /locus_tag="K0W65_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBI3096384.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2968..3615
                     /gene="gloC"
                     /locus_tag="K0W65_003236"
     CDS             2968..3615
                     /gene="gloC"
                     /locus_tag="K0W65_003236"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBI3096385.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3838..5028)
                     /gene="aspC"
                     /locus_tag="K0W65_003237"
     CDS             complement(3838..5028)
                     /gene="aspC"
                     /locus_tag="K0W65_003237"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HBI3096386.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5213..6301)
                     /gene="ompF"
                     /locus_tag="K0W65_003238"
     CDS             complement(5213..6301)
                     /gene="ompF"
                     /locus_tag="K0W65_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBI3096387.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6902..8302)
                     /gene="asnS"
                     /locus_tag="K0W65_003239"
     CDS             complement(6902..8302)
                     /gene="asnS"
                     /locus_tag="K0W65_003239"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBI3096388.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8471..9673)
                     /gene="pncB"
                     /locus_tag="K0W65_003240"
     CDS             complement(8471..9673)
                     /gene="pncB"
                     /locus_tag="K0W65_003240"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBI3096389.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9939..12554
                     /gene="pepN"
                     /locus_tag="K0W65_003241"
     CDS             9939..12554
                     /gene="pepN"
                     /locus_tag="K0W65_003241"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBI3096390.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12761..13528)
                     /gene="ssuB"
                     /locus_tag="K0W65_003242"
     CDS             complement(12761..13528)
                     /gene="ssuB"
                     /locus_tag="K0W65_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBI3096391.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13525..>14138)
                     /gene="ssuC"
                     /locus_tag="K0W65_003243"
     CDS             complement(13525..>14138)
                     /gene="ssuC"
                     /locus_tag="K0W65_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HBI3096392.1"
                     /translation="HLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQML
                     RNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARSYG
                     LSGIPLFIHVILPDALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQ
                     TDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATI"
BASE COUNT         3602 a         3605 c         3576 g         3355 t
ORIGIN      
        1 ttcaccggga cgtataccgg agctgatgcg cgatcttgca agtatgggac aacttgtgaa
       61 attaccgacg agaaggggaa gggccttccc gagagtggta aaggagaggc cctggaaata
      121 ccccacagcc ccgaaaaaga gccagtcagt tgcttaactg actggcatta cacataacgg
      181 cgcgcttttc tttaaacttc tttacattag gttatgtaaa aacgtatcgg cgtttatata
      241 ctgaagataa gcctgatgag taacaggctt gctcgtcata ctttcgtgag tattggcgtt
      301 gtacaggcaa gtcgtaaaat aacagcctgg ctattcagag tatgataaaa acagggggca
      361 agggatgttg cttaatatga tgtgtggtcg tcggctgtcg gcaatcagtt tgtgcctggc
      421 cgtaacattc gctccactgt tcaatgcgca ggccgatgag cctgaagtaa tccctggcga
      481 cagcccggtg gctgtcagtg aacagggcga ggcactgccg caggcgcaag ccacggcaat
      541 aatggcgggg atccagccat tgcctgaagg tgcggcagaa aaagcccgca cgcaaatcga
      601 atctcaatta cccgcaggtt acaagccggt ttatcttaac cagcttcaac tgttgtatgc
      661 cgcacgcgat atgcaaccca tgtgggaaaa ccgtgatgct gttaaagcct tccagcaaca
      721 gctggcagag gtggcgattg ctggtttcca gccgcagttt aataaatggg tagagttact
      781 gaccgatcct ggtgttaacg ggatggcacg tgacgtggtg ctctctgatg cgatgatggg
      841 ctatctccat ttcattgcaa atattccgat caaaggcact cgctggctat atagcagtaa
      901 accttatgcg ctttcaacgc cgccgctttc ggtgattaac caatggcagc tagcgctgga
      961 taaaggtcaa ttgcctacgt ttgttgcagg actggcaccg cagcatccgc aatatgcggc
     1021 gatgcatgaa tcgttactgg tcttactcag tgacaccaaa ccgtggcccc aactgaccgg
     1081 caaagcaacg ttgcgcccag ggcagtggag taacgacgta ccggcgttgc gcgaaatatt
     1141 gcaacgcaca ggcatgttgg acggggggcc gaaaattact ctacctggcg atgacacgcc
     1201 aactgacgcg gtagtcagcc catccgctgt tactgttgaa acagcagaaa ctaagccgat
     1261 ggacaagcaa acgacgtctc gtagtaaacc tgcgcctgcc gttcgtgccg cctacgataa
     1321 tgaactggtg gaagccgtta aacgttttca ggcatggcaa ggattgggag cagatggtgc
     1381 tattggcccg gcaacgcgtg actggttaaa cgtaacgccc gcccagcgtg ctggcgtgtt
     1441 ggcactcaac atccagcgat tgcgcttgct gccaacagag ctttctaccg ggatcatggt
     1501 taacattcca gcctattcgc tgctctacta tcagaacggc aatcaggtgc tggattcgcg
     1561 agtcattgtc ggtcgccccg atcgcaaaac gccgatgatg agcagtgcac ttaacaacgt
     1621 agtggtaaac ccgccgtgga acgtaccacc aactctggca cgcaaagata ttctgccgaa
     1681 agtgcgcaac gatccgggat atctcgaaag ccatggctat acggtgatgc gcggctggaa
     1741 cagcagagaa gcgattgacc catgggaggt tgactggtct acaatcacgg cctcgaattt
     1801 accgtttcgc ttccagcagg ctccaggccc acggaactcg ctggggcgct ataaattcaa
     1861 tatgccgagt tcagaggcca tttatttgca tgacacgccg aaccacaatc tgttcaagcg
     1921 tgatacacgc gcattgagct caggctgtgt acgagtgaat aaagcttccg atctggcgaa
     1981 tatgctgttg caggatgcag gatggaatga caaacgtatt tctgatgcgc tgaagcaggg
     2041 tgatacacgt tacgtcaata ttcggcagtc gattccggtg aatctctact acctgacggc
     2101 ctttgttggt gcagatgatc gtacccagta tcgtacagat atttacaatt atgatctgcc
     2161 tgcgcgatcc agctcgcaaa tcgtatcgaa agcggaacaa ttaatcaggt aaatgaagta
     2221 gttcggggaa atcaattgtc gtaataatta gcatgattgg gggcgattct ctgcagcccc
     2281 cgtcactgct ggggttgagt catcttgacg tctgctttac gggcggttaa ggtgcctctt
     2341 gtgcgccaga agtgcatata aacgataaca ttgagctgta gacttgatta tcatggacaa
     2401 attcgacgct aatcgccgca aattgctggc gcttggtggc gttgcactcg gtgccgccat
     2461 cctgccgacc cctgcgtttg caacactctc taccccacgc ccgcgcattt tgacactcaa
     2521 taatcttcat accggagagt caatcaaagc ggagtttttc gatggcagag gctatattca
     2581 ggaagaattg gcaaaactta accatttttt ccgcgattac cgcgcgaaca aaataaagtc
     2641 catcgaccca ggattattcg accagttgta tcgcctgcaa gggttgttag gcacgcgcaa
     2701 accggtgcaa ctcatttccg gttatcgttc cattgatacc aacaatgaac tacgcgcccg
     2761 cagccgtgga gtagcgaaga aaagctatca cactaaaggc caggcgatgg atttccatat
     2821 tgaaggtatc gcgttaagca atattcgcaa agccgcgtta tctatgcgcg caggtggtgt
     2881 aggatattac ccacgtagta actttgtgca tattgatacc gggccagcac ggcactggta
     2941 gtaatcgctt aacgaaacag gggcagtatg aactatcgta ttattccggt caccgcattc
     3001 tcccagaact gttcattgat ctggtgtgaa caaacccgcc tggccgcact ggtcgatcct
     3061 ggcggcgatg cggaaaaaat caaacaggaa gttgatgaca gcggcctgac actgatgcag
     3121 atcctgctga cgcatggtca tctggaccac gttggcgcag cggcggaact ggcgcaacat
     3181 tacggcgtgc cggtttttgg cccggaaaaa gaagatgagt tctggctgca aggcttgcct
     3241 gcgcaaagtc gtatgtttgg tctggaagag tgccagccgc tgacgccaga tcgttggctg
     3301 aacgaaggcg ataccatcag catagggaat gtgactttac aggtgttaca ttgccctggg
     3361 catacgccgg gtcatgtcgt gttttttgat gatcgggcaa agctgctgat ttctggcgat
     3421 gttattttca aaggcggagt agggcgcagt gacttcccgc gtggcgatca taatcaactg
     3481 atttcttcaa tcaaagataa attgctgcca ctgggggatg acgtgacatt tattccgggt
     3541 cacggaccat tatctacact tggttatgag cgcctgcaca atcccttcct gcaagacgaa
     3601 atgcccgtct ggtaaaggcg cataaaaaag tccactttta atgcgggcct ggatttctgg
     3661 caaagtgcgc tttgtttatg ccggatgcgg cacgagcgcc ttatccggcc tacaaaatcg
     3721 tgcaaattca aaatattgca ggggacgcgt aggcctgata agcgtagcgc atcaggcaat
     3781 gttgcgtttg tcatcagtct cagcccgctt ttcagcgggc ttcattgttt ttaacgctta
     3841 cagcactgcc acaatcgatt cgcacagcgg agccatgtta tctggtgtca tcccggccac
     3901 atttacgcga ccagaagcaa cagcatatac gccaaactct tcgcgcagac gcagcacttg
     3961 ttcttttgtc agaccactga aggagaacat gccgttctgt ttgatgataa agctgaagtc
     4021 gcggtttgca cctttttcct gcagcgtatt gacgaacaac tgacgcatac gctgaatacg
     4081 ctggcgcata tcagtcagct cttgttccca aatcgcacgt aatgcatcgc tgctcaggat
     4141 ggtggcaaca acagaagcgc cgtgtgctgg agggttagag tagttagcgc gaatcgccgc
     4201 tttcatttgg ctgaatgcgc gatcaacgat ttcactgtcg gcagcaacca gagtacaagc
     4261 gccaacacgc tcgttgtaca ggccaaagtt tttagagtag gaactggcaa caatcagctc
     4321 tttatgcata gccgcgaaag cgcgcagtcc ttcagcatct tcttccagac cacgggcaaa
     4381 accctggtaa gcgaagtcaa acagcggtaa ccagcctttc tcaacggaga gttgtgccag
     4441 tgtctgccat tgttccagcg tagggtcgat accggttggg ttatggcagc agccatggaa
     4501 cagcactacg tcgccagcct gagcttcgtt cagactgtta atcagtgcat cgaagtcaag
     4561 ggtgtgattt tccgcatcat aataagcgta ttcacgaact tccagacctg cagagttaaa
     4621 gacgctctta tggttcggcc agcttgggtt gctcacccac acacgcttaa cgctggtatt
     4681 ttttgccagg aagtcggcag ccacgcgtag tgcgccagtg ccacccggag tctgtgccgt
     4741 gcgagcacgt ttgtcattga tcagggcgct acctttacca aacagcagtt cctgagtgca
     4801 gcgaccaaat tcaaggatgc cgtcaatgcc gaggtaattt ttggtggttt cattttcgag
     4861 cagatactgt tcagcctttt tcacgctggt cagtaccggg gttttgcccg tctcatcttt
     4921 atagacacca atcccgaggt taattttgcc gggacgttca tcggcacgaa acagatcggc
     4981 caggcccaga atcgggtcgg caggagcggc ggtaatgttc tcaaacatga cgaggttcca
     5041 ttatggttac agaagggaag tccgctatca gggtaacggg agatttacaa aattccaact
     5101 attactgatg aaaacgcagg ctgtttttgc aagacgtgag attgctctgg aaggcataaa
     5161 aaaaacagga ccaacgtcct gttttttcgg catttaacaa agaggtgtgc tattagaact
     5221 ggtaaacgat acccacagca acggtgtcgt ctgaacctac gcccagtttg ttgtcagaat
     5281 cgatctggtt gatgatgtag tcaacatagg tggacatgtt tttgttgaag tagtaggttg
     5341 cgcccacttc aaagtagttc accagatcaa catcaccgat accttctacg tctttcgctt
     5401 tagatttggt gtaagcgatg gacggacgca gaccgaaatc gaactggtat tgcgcaacta
     5461 acagaacgtc ttgcgttttg ttggcgaagc cgctggtgtt tgtaaattta ttagtgatcg
     5521 gcgtagcgtt acgggtttca ccgtagttcg ctgccaggta gatgttgttc gcgtcgtact
     5581 tcagaccagt agcccactgt tcagcttttt taccgttgcc aagaggttga gcttcttgca
     5641 ggttggtacg gtcagctgca ccataagcac caacgatacc aaagccttcg tattcgtagc
     5701 tgatagaacc gccaacaccg tcgccgttag aacggcgtgc agtgtcacgc tcgtttttac
     5761 ccaggtactg aacagcgaag ttcaggccat caaccagacc aaagaagttg gagttacgat
     5821 aggtagcaac gccgccaaca cgaccaacga agaagtcatc gctgtatgca gtatcaccac
     5881 caaattctgg cagcatatcg gtgtaaccca gtgcatcata aaccacaccg tagttacggc
     5941 cgtaatcgaa agaaccaacg tcagcgtatt taagacccgc gaatgccaga cgcgttttgt
     6001 taccagtttg agcgtcagcg ccttcagagt tgttaccctg gaagttatat tcccactgac
     6061 cataaccggt cagatcggaa ttgatttgag tttccccttt aaaaccaaga cgggcatagg
     6121 tcatgtcgcc attgccaccg taactgtttt caccgttacc cttggaaaaa taatgcagac
     6181 caacagtttt accgtacaga tctactttgt tgccatcttt gttatagatt tctgcagcgt
     6241 ttgcagtacc tgctactaac agagcaggga cgatcactgc cagaatattg cgcttcatca
     6301 ttatttatta ccctcatggt ttttttatga cacctgccac tgccgtcaat aagttctgtc
     6361 aataaaaatt tacggaacta ttgatgagag tttggtgtct ttatgtgtct gcaggcatct
     6421 ttccattcaa actaacgttt cgctaccgtg aaagtgctac aaagataaag atttagtttc
     6481 aaaaagaaaa aatatgtaac caaaagtaaa aattaaggag ctttgtgaac accgtcatat
     6541 ttccatagaa acgtgatgat atttacagca atttttatct atttatatga tttacttata
     6601 tttaaattaa ctaaacggaa attttgtttc tgatggaaac tttatcgacc tggcacaaaa
     6661 tcctctttca gacatccaga atgccaaaaa atagtatgaa aattgtgcta tcagctaaga
     6721 aaaaactaat ccgcaataag agtacgggtt attttttgtg cgtaaatgtt gcaatgtaat
     6781 attccgtaac aggatgatcg ttatggcggg ggtgcaaata gattgcttga cttaacaaac
     6841 agataacttg acagaaagga taaaaaagag ccagcaggcg ctggctcttt gaggaagaaa
     6901 atcagaagct ggcgttgcgc ggagtacgtg ggaacggaat cacatcacgc acgttttgca
     6961 cgccagttac gtaagcaatc aggcgttcaa aaccaagacc gaaacctgaa tgcggaacag
     7021 taccgtagcg acgcagatcg cgataccacc agtaatcttc tttattcagg cccatttcca
     7081 gcatacgctc gtccagaacg tccagacgtt cttcacgctg ggagccacca atgatctcac
     7141 cgatgcccgg agccagaacg tccatagccg caacggtttt accgtcttcg ttgaggcgca
     7201 tatagaacgc tttaatatct ttcggatagt ttttaaccac taccggtgct ttaaagtgtt
     7261 cttccgccag ataacgctca tgctcagaag agagatcgac gccccagtaa accgggtttt
     7321 caaacttcct gccgcagttt tcgagaatgg tcactgcgtc ggtgtaatcc acctgcgcga
     7381 aatcggcttc aatgaagcgt tccagacgtg aaacggcatc tttatctacg cgttcagcga
     7441 agaatttcat gtcgtctgcg cgttcttcga gaaccgcttt gaagacatat ttcagcatgg
     7501 cttcagccag acccgcgata tcgttcaggt tagcaaacgc cacttccggc tccagcatcc
     7561 agaattccgc caggtgacgg ctggtgttgg agttttcagc acggaaagtc gggccgaagg
     7621 tataaatttt ggataatgcg caagcgtagg tttcgccgtt caactggcca gatacggtca
     7681 ggaaagactc tttaccaaag aagtctttgt cgaaatccac tttgccctga tcgttacgcg
     7741 gcaggttttc cagatccagc gtagaaacgc ggaacatttc gcctgcacct tcggtatcag
     7801 atgcggtaat cagtggcgtt gaaacccaga agaatccctg ctcgttaaag aagcgatgca
     7861 gcgcctgcgc cagcgtatgg cgaacgcgcg cgacggcacc aatcaggttt gtgcgcggac
     7921 gcaggtgagc gacttcacgc agatactcaa tgctgtggcg ttttgccgcc atcgggtaag
     7981 tgtctggatc ttcaacccaa ccagcaactt caaccttgct ggcctgaatt tcaaattgtt
     8041 gcccctggcc cggcgatgcc acgactttac ccgtcacaat gaccgagcag ccggtggtca
     8101 gacgcaggac gtcttcattg taattgggca gagaattatt gatgacagcc tgtacaggat
     8161 caaagcagga accgtcataa acggcgagga aggagatgcc agcttttgaa tctcggcggg
     8221 tacgtaccca tccgcgtacg gtgacttcgc tgtcaacggc tacgcggccc tggagtacgt
     8281 cggctacagg cacaacgctc ataatattct ctctgttaat agtcggaaaa aataaacact
     8341 tgtccacccg taaatggggg ggatacctat gttacctggc atctgcaatc agacaagcag
     8401 aattcgcaaa tgcagcgaaa gatttcggaa ttaaaggtaa aaaagggagc cgattagctc
     8461 cctgatgata ttaactggct tttttaatat gcggaaggtc gaacgctttg cgcagcgccc
     8521 gaacaaacgc tttatcatgg cagatagttt tgccagggct gtcagaaagt ttcgccaccg
     8581 gtttaccgtt acactctacc aacttaatca caatattcag gggttttacc tgggggatat
     8641 cgcaggtcag gcgagtccca ataccaaaac ttaattgcac gcgggaagag aagtggcggt
     8701 atagctcaac cgctttgcgt aaatccagat tgtcagagaa aaccagcgtt ttactctgtg
     8761 gatcaattcc cagcttttca taatgtgcaa tggctttttc accccattca accgggtcgc
     8821 cagagtcatg acgcaggccc tgataccgac tagcgaactc gacaccgaaa tcacgcagga
     8881 aagcatccat agtgatgcag tcggttaatg caatgccaag ttggtcggga tactcttcca
     8941 gccaggcagc aagtgcagct cgctggctgt tggctagatc cgggctgatt tgctgatgtg
     9001 cctggaacca ttcgtgtgcc tgtgttccca tcggcgtgag ggaaagccga cgcgccagat
     9061 cgtagttgct ggtgcccacg aaccaggatt cctgttgcag acgcttaacg atggtttctt
     9121 gtacttcgcg agaaaaacgg cgacgggtgc caaaatccat cagatggaag cgcgacatat
     9181 caagaccggc ggttaacgcc gagaagtcga ctaatttgct ttccagcgtg tcgagggctt
     9241 gcgcaacgtc ggcctgcggt gagcgatagc gatgtaccat ttcactgata accgccagca
     9301 aaggaacttc ccagaggatg acttcacgcc acggaccgct taagcgaata tccagcttgc
     9361 cattatcgtt ggacacggtg acttgttccg ggttaaagcg gaactcgcgt aaccagttaa
     9421 gatagtcggc cttaaagaaa ggcagggcag agagccactg atattcatca tcctgcaggc
     9481 gcaggtgctg catcgcctga atctgttcac gaatagcatc ggcataaata cccagcagat
     9541 cgtcacctcg gcaacgaaac tccgccgcga catgcacatc gtaatagtga tgaaacacgg
     9601 cttgctgcat atgcaactta taagcatctg tatccagcaa cgagtgcaga acaggagaag
     9661 cgaattgtgt cataggtgcg cagtagcgtc ctcgtacagg agcgtttagt acaataaaca
     9721 tcttcaggaa ctgctggagt ataccttgtt tcgcaattta ttgaaccccg atcacaccat
     9781 atgccacctt tctggtcgat ggcattacgc ggtgtatgtt ataaaaatgt agcaataaag
     9841 gcgtttgtac ctgaaaagat gaagattctg catagcgcga tttacgcaac aggaatagac
     9901 tgaacaccag actctataaa agatgctaaa ggttatttat gactcaacag ccacaagcca
     9961 aataccgtca cgattatcgt gcgccggatt accagattac tgatattgac ttgacctttg
    10021 acctcgacgc gcaaaagacg gtcgttaccg cggtcagcca ggctgtccgt catggtgcat
    10081 cagatgctcc gcttcgtctc aacggcgaag acctcaaact ggtttctgtt catattaatg
    10141 atgagccgtg gaccgcctgg aaagaagaag aagagggcgc actggtcatc agtaatttgc
    10201 cggagcgttt tacgcttaag atcattaatg aaataagcct ggcggcgaat acggcgctgg
    10261 aagggcttta tcagtcaggc gatgcgcttt gcacccagtg tgaagccgaa ggtttccgcc
    10321 atattacgta ttatctcgac cgcccggacg tgctggcgcg ttttaccacc aaaattattg
    10381 ccgataaaat caaatatccc ttcctgcttt ccaatggtaa ccgcgttgcg caaggcgaac
    10441 tcgaaaacgg acgccattgg gtacagtggc aggacccgtt cccgaaaccg tgctacctgt
    10501 ttgcgctggt ggcaggcgac tttgatgtac tgcgcgatac ctttaccacg cgttctggtc
    10561 gcgaagtagc actggagctg tacgtcgatc gcggcaacct tgatcgcgcg ccgtgggcga
    10621 tgacctcgct gaaaaactcc atgaaatggg atgaagaacg ctttggcctg gagtatgacc
    10681 tcgacatcta tatgatcgtc gcggtggatt tcttcaatat gggcgcaatg gagaataagg
    10741 gtctgaatat ctttaactcc aaatatgtgc tggcccgcac cgacaccgcc accgacaaag
    10801 attacctcga tattgaacgc gttatcggcc atgaatattt ccataactgg accggtaacc
    10861 gagtgacctg ccgcgactgg ttccagctca gcctgaaaga aggtttaacc gtcttccgcg
    10921 atcaggaatt cagctctgac cttggttccc gcgcagttaa ccgcatcaat aatgtgcgca
    10981 ccatgcgcgg attgcagttt gcggaagacg ccagcccgat ggcgcaccca atccgcccgg
    11041 acatggtcat tgagatgaac aacttctaca ccctgaccgt ttacgagaag ggcgcggaag
    11101 tgatccgtat gatccacacc ctgctgggcg aagaaaactt ccagaaaggg atgcaactct
    11161 atttcgagcg tcatgatggt agcgcagcga cctgtgacga ctttgtgcag gcgatggaag
    11221 atgcgtcgaa tgttgatctc tcccatttcc gccgttggta cagccagtcc ggtacgccgg
    11281 ttgtgaccgt caaagacgac tacaatccgg aaactgagca gtacaccctg accatcagcc
    11341 agcgcacgcc agccacgccg gatcaggcag aaaaacagcc gctgcatatt ccgtttgcca
    11401 tcgaattgta tgacaacgaa ggtaaagtga tcccgttgca gaaaggcggt catccggtga
    11461 attccgtgct gaacgtcacc caggcggaac agacctttgt ttttgataat gtctacttcc
    11521 agccggtgcc tgcgctgctg tgcgaattct ctgcgccagt gaaactggaa tataagtgga
    11581 gcgatcagca actgaccttc ctgatgcgtc atgcgcgtaa tggtttctcc cgttgggatg
    11641 cggcgcaaag tttgctggca acctacatca agctgaacgt cgcccgtcat cagcaagggc
    11701 agccgctgtc tctgccggta catgtggctg acgctttccg cgcggtgctg ctcgatgaga
    11761 agattgatcc ggcgctggcg gcagaaatcc tgacgctgcc ttctgtcaat gaaatggctg
    11821 aactgttcga tatcatagac ccgattgcta ttgccgaagt acgcgaagca ctcactcgta
    11881 ctctggcgac tgaactggcg gatgagctgc tggctattta caacgcgaat taccagagcg
    11941 agtaccgtgt tgagcatgaa gatattgcaa aacgcactct gcgtaatgcc tgcctgcgct
    12001 tccttgcttt tggtgaaacg catctggctg acgtgctggt gagcaagcaa taccatgaag
    12061 caaacaatat gaccgatgcg ctggcagcgc tttctgcggc ggttgctgca cagctgcctt
    12121 gccgtgacgc gctgatgcag gagtacgacg ataagtggca tcaggacggt ctggtgatgg
    12181 ataaatggtt tatcctgcaa gccaccagcc cggcggcgaa tgtgctggag acggtgcgtg
    12241 gtctgttgca gcatcgctca tttaccatga gcaacccgaa ccgtattcgt tcgttgattg
    12301 gtgcgtttgc gggcagcaac ccggcagcgt tccatgccga agatggcagc ggttaccagt
    12361 tcctggtgga aatgcttacc gacctcaaca gccgtaaccc gcaggtagct tcacgtctga
    12421 ttgaaccgct gattcgcctg aaacgttatg atgccaaacg tcaggagaaa atgcgcgcgg
    12481 cgctggaaca gttgaaaggg ctggaaaatc tctctggcga tctttacgag aagataacta
    12541 aagcactggc ttgatacttt tgagccaaaa tgcctgatgc gacgcttgac gcgtcttatc
    12601 aggcctacaa ctccgttgaa attgctggtt ttgtaggctg gataaggcgt tcacgccgca
    12661 tccggcagcc gtgcccaact gcctgatgcg atgcttgacg tgtcttatca ggcctacaag
    12721 tccgttgaaa atgctggttt tgtgcgccac atccggcata ttaaccatga cgccgaatca
    12781 gttgttcaga atgcccacgc cgcattaccc gttgcaatac ttccgcttcc agctccgcta
    12841 atcgcaccga ccctaatcga cgcggacggg ggatatccac tgtcaaatcc agactaattt
    12901 ttccctcttc aattaacaac acccggtcag ccattgctac cgcttcgctc acatcatgcg
    12961 tcaccaacag tacggtaaag ccgtgctgct gccaaagtga cacaatcaaa tcctgcatct
    13021 cgagtcgcgt taaggcgtcc agtgccccga gcggttcatc aagcaacaat aaacccggtc
    13081 gatgaatcaa cgctcttgcc agcgccacac gctgtttctg cccgccagaa agtgcggcag
    13141 gccattcccc ggcgcgattc tccagtcctg ccgcagccaa cgcccgacgc gcggcatcgc
    13201 gccactgacc tttaaggcct aacccaacgt tatcaatcac cgatttccac ggcagcagac
    13261 gcgcatcctg aaacatcatt cgcgtatcat cctgaatttc agccagcggt gtggtgcccg
    13321 ctaacacatc gcctgcggtt ggcgtttcca gaccggccag caggtgcagc agggtacttt
    13381 taccaccacc gctgcggccc accaccgcca caaactgacc tgccggaata tgtaaatcca
    13441 gttggttcag gacgatattt tccgcgtaat gtttgcttac tgcattgagc aacaatggtg
    13501 tgccctggtt cagacgagca gtattcatat cgtggcctcc ttcaaatgat aagccgggtt
    13561 ccagcgcaac cagaggcgct ctaacagctg cgcgctgacg tcagcaagtt tgccgagcag
    13621 ggcgtaaaga ataatagcga ctaccaccac gtctgtttgc aaaaactctc gcgcattcat
    13681 cgccagataa ccaatgccgg aattggcaga aatggtttcg gcaacaatca gcgtcagcca
    13741 catcaggccc aacgcaaaac gcacgccgac cataattgag ggcagggcat cgggcaggat
    13801 cacatggata aacagcggta tgccggataa tccatagcta cgcgccatct ccaccagccc
    13861 gcgatcgata ttacggatgc catgccaggt gttgatataa atggggaaca gcgtaccgag
    13921 cgccaccaga aagattttcg cggattcatc aatgccaaac cacaaaatca ccagcgggat
    13981 cagcgccaga tgcggcacgt tgcgcaacat ctgaattgag gtatccagca gcctttctcc
    14041 ccagcgcgac agtccgctaa tcagtcccag aatcaggccc agcgatccgc caattgaaaa
    14101 gccaatcagc gcccgccagg aactgatcgc cagatgct
//
LOCUS       DAETQL010000094        13989 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.094, whole genome shotgun sequence.
ACCESSION   DAETQL010000094 DAETQL010000000
VERSION     DAETQL010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.094"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            559..1350
                     /locus_tag="K0W65_003244"
     CDS             559..1350
                     /locus_tag="K0W65_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096393.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1736..1942
                     /locus_tag="K0W65_003245"
     CDS             1736..1942
                     /locus_tag="K0W65_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBI3096394.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            2028..2600
                     /locus_tag="K0W65_003246"
     CDS             2028..2600
                     /locus_tag="K0W65_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096395.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2602..2886)
                     /locus_tag="K0W65_003247"
     CDS             complement(2602..2886)
                     /locus_tag="K0W65_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096396.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            2857..3000
                     /locus_tag="K0W65_003248"
                     /pseudo
     CDS             2857..3000
                     /locus_tag="K0W65_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            4169..5188
                     /locus_tag="K0W65_003249"
     CDS             4169..5188
                     /locus_tag="K0W65_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3096397.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
     gene            5298..6170
                     /locus_tag="K0W65_003250"
     CDS             5298..6170
                     /locus_tag="K0W65_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HBI3096398.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            6504..9626
                     /gene="ag43"
                     /locus_tag="K0W65_003251"
     CDS             6504..9626
                     /gene="ag43"
                     /locus_tag="K0W65_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HBI3096399.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHG
                     SAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            9747..12263
                     /locus_tag="K0W65_003252"
     CDS             9747..12263
                     /locus_tag="K0W65_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096400.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            12339..12794
                     /locus_tag="K0W65_003253"
     CDS             12339..12794
                     /locus_tag="K0W65_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096401.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(12913..13124)
                     /locus_tag="K0W65_003254"
                     /pseudo
     CDS             complement(12913..13124)
                     /locus_tag="K0W65_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13205..>13989
                     /locus_tag="K0W65_003255"
     CDS             13205..>13989
                     /locus_tag="K0W65_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBI3096402.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     TYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWV"
BASE COUNT         3766 a         3462 c         3450 g         3311 t
ORIGIN      
        1 aaataacaga cattacttaa caaagaacat tacagtcagt gtgtacatta cagatttata
       61 ttcaggtgtt gcgggactac attacagcag agaggggtaa ggacagagtt acaagtacta
      121 ctagagagaa tatcactgca cagatacttc tatgagtata tctttatgag agatccgtct
      181 acaggattgg gttataaggt gtatttacaa ggtataatca caggatcatt tacatgacca
      241 tacttctgta aataatacta attaacctta attatttttt tatggtacat gaactacctg
      301 tagctgtaac gataaacaaa tcacccaaaa tgaaaaatac tggttatcac agttcagaga
      361 actcgtatga gtgtccccct gagcaatgct tcattttctg tgattctgtg tgatagagtt
      421 atactgagta cagtggcagt aacactgctt tccatcactt gaggataaaa ttcattaaaa
      481 aattaattgc atgaattaac aggttttttt atgaaatgtt atgcatctca accaatatga
      541 ttaacagaaa acttcatcat gacaacacgt ataacgttca gtatgaccag tgatgagacg
      601 ctgcgaattg tggatgagta ctgtcatacc cacaaacttt cgcgctctaa ggtaatcaac
      661 gcattactca gcgccacagc acctgtcctt aatgatatta actgttatta tcaacttgca
      721 ggcgaactgc agtcacgctt attaaatgga gtgtatcaac gggatcttcc tcgcaaacgc
      781 aatgtcgtca gtgcagagaa gtattgtctg gaaatctggg aaaacaaact tttcaccaga
      841 agaatcctgg agtttgacag ttcaaatggt gttctgtacg cgctgaaaca taaaagacac
      901 tatcgccggg ataagatgat tggaagggtt gaaagtcggt gtatcaaaga catttgcgaa
      961 taccagatgc agctatccgg ggagaagacg aaatatgcct gttttattta tatcgaaaga
     1021 acaatataca atcacgataa tccatctgat aaaatacctg taaaagcggc tgttggtaat
     1081 gcagtcatcc tgctggcaaa ggatgtgata tataacgagt acttttttga tttgcggcag
     1141 tcattttttg tgagcgttac agatctgatg gtctctggcg caaaaggtat tccggagaca
     1201 cagacatatc cggacgttta ttgctggatc cctctttttt ccataaattc cggcgtgctc
     1261 attacccctg tatataaaat tgaccctctg aaacccgtaa cggtaaaaaa accggataag
     1321 atcactgttg tttgtaatta cagagaataa tttcagcctt ctaccttcac cgcgacagtg
     1381 tttacctctg ctttatcttc cgttcagtaa atacaaacca ccgctctggc aggtacaggg
     1441 cgtggtttat ctccgatact cccctgtgca gtaataatca tcactgaaaa tactgatccc
     1501 gtcacggcaa aacgccatcc catattccgg caacacaaaa tcatcttccg tttataccgg
     1561 attcactgac aaaacagaca acctgtattg cctgtacaca acagtttctc tcctggcagg
     1621 aatattcccc tactcatttt ttacgtttct ccccggttgt tatgccggtg aaggtggtgc
     1681 gtcgttttca tcaccaacgc cggttgagat taacaacatc cggaggaaca ttctcatgaa
     1741 taccccagtt tcactgatgg atgaccagat ggtcgacatg tcgtttatca ctcagctgac
     1801 cggcctgacc gataagtggt tttataaact catcaagcat ggagcctttc cgccccccat
     1861 caaactgggc cgtagctccc gctggctgaa aagcgaagtg gaagcctggc tgcaggcgcg
     1921 tattgcacag tcccgtccgt aattcctgac cgttatccgt tccaccgcag caaaggcatg
     1981 atccggcccc cggaggcatt ttgctgcgcg ctaaaggaga gaatattatg caacaacttg
     2041 aaaacaggtg tgacctgctg ctcatccagc atcagaaatg gatgacaagc gtgacgaggc
     2101 ttatcgtcgc tcatggaatg gggtctccgc atcttcatgg ttatcaccgt ctgaccctgg
     2161 cacacttttt tctgccggaa aaaggctccg tcatttcggt ggccccccag ggactctatc
     2221 aggtcgtcaa cccgggaaca ccaccgttca ttcctgccat tcaggaaggg ttaatgacca
     2281 gcattcagac ccatgaaatc atgctgctta cacattttaa tctgggcggc gttttactca
     2341 gtgagctaca tcgtctgggt gaaaaccggc tggcaaacag gctgaacagc ctgctccggc
     2401 gtttcgacga tcgtgatctc taccacacgc tgatatggct gtgctggtat gatctgatgt
     2461 gtgcccactc catgcagccc tggactgaag agttaaagca taaatcacat gcagagcttg
     2521 aaaactgggc tgtggcgcgc aaacgggaaa agcgcgagct ggaactgatg attgacgagt
     2581 acctgttata cgcctgctga ttcataacgt tcccggggtt ccttcctcat cgttacacac
     2641 agccggacgg ctatctatgg ctgtccgcca aatgaccatc gccgcatttc gttccgctga
     2701 aaacggtacg tagtcagtga taccggaatt tccggagagg gccgcaggta tattgcttct
     2761 gtgcaattga tgcttattta cattggatgt agttatttct gcatccaatg ctatcggaaa
     2821 gagattaacc agcaaatggt tcctcaaatc gtattgatat ttacccggcg tattgagcac
     2881 caccatctga cactccgttc ccgcatcaaa cgactggacc gaaaaacgat ttacttttca
     2941 cgtgctgtgg aaattcacga aaaaattatt gggtcattca tcgaaaaaca tatgttctga
     3001 tgactggagt catcctccag aaatatacgt caactgaaca atatcgattt tttatgggat
     3061 tctatacact tttccatatc cctctgaaaa tcagtcatca gctcaccaac acctcttctg
     3121 aaacttgtat taaacttatt gcttaaatag atgatataac atctcttcca ttgcactgaa
     3181 tcggcgaaaa ctgccgtttc aataatctca acaatattat ttatcggcta actttatcag
     3241 taaacaatgg aatgtcacac tgatgaaatg aacattcaga gtaagatgat aaaattcata
     3301 taaacgccat tgctgaatat tatttatttc atacagataa acgataaaat acaaaagggt
     3361 atatttctca atgtaacagt caattattca tctccactgg cgagcacggt cagcacacaa
     3421 acaaagttag tcctcctgct taatcattgc gtatcagaaa ctaaaaaaga aaaaattctg
     3481 cagacaactt aacaatcttc atcacatttc ctgaagacag acatatccac aaacggtagt
     3541 cagcgttgaa ttaacaagtc ttacagagct atacgtagct tataaagcca ctcaatgttc
     3601 atcatcaccc gactacttat ctggaatacc accagtataa actcatcaat aaaaacacct
     3661 gttgttaaaa taagaatact gtcagcagta aattattaaa acaacatctg tccacactat
     3721 ctgttctttt aacgccaacg gcagcgaaaa tagtgcatca cagccttctt ttttctttcg
     3781 ccaccactca gacaaacaca acactcacac ccacaccaca aaacagaatc aaaacataaa
     3841 attaacaata acagtcaaaa ggaaaacaca gaatattgag gtggactgtc accaggacag
     3901 cgcatacgac gcatgtgatc agcacatccc gttcttcgct ggcatcccaa aaatgcagaa
     3961 caaacaatca taacattatg aatataaaag aaaatatgat ttttcaccat gaaaaaaaca
     4021 tcaatgtatc tctgtataaa aataatgata acatgacata aacatttaaa aacataaata
     4081 aacagcaaca aaaacatttg ataatcactt agtgattaaa tacaattaag acgattttgt
     4141 tataagagag gcaccatgga gtgagataat gaataacgaa cagatgctta tcaaatgcag
     4201 agatccccgt aaatgtcggt tttatgccga atatctgaat cacaaatacc ctaatgaatt
     4261 ccctcacttt gcgcctgaca acccacaaaa ttgtatcagg aatgttaatt tatttttaaa
     4321 ggaaaagtat tgtaatgttt cacctccccc ctttggtact tttggtacta ctgaagaaga
     4381 agcactcaat aacgaaatta atgagcttga aattattgcc gggcaggcaa tactgcctga
     4441 agagagtttt ctctggttga aaactgacga acttgcgact ttctttacct ggactactat
     4501 ttatctgtcg aaggaagacc atacaaaatt cccgaaaata aaccataatt catcaggaat
     4561 aaaaaacgga attgaagtca ggaaaatacg aacagttacc gatgaaacgt taaacactta
     4621 caaaaaaagt aaccttccgg ataatccatc atcccataac gaacgaataa aagtaatcat
     4681 ttcatatttt gactcctgcc attctttaat taataaaaat gcaaaaagca tatacctcga
     4741 ggaactcagg caggcgtggc ttaaaaagaa aagcgcttgt caaaagttta agtggctcct
     4801 tcctgacgat gatgaaatgt gtcagtggtt ctggacaaga ctgaaacaaa aacaggatga
     4861 aaaacatcca gtacataacc cagtgacccg gtggttcata ccatcttcaa cgaaagaata
     4921 ttatcttgcc tctcttgtcg cctttgcttt atggaaagaa gcgcctgata ccattgaact
     4981 gttcagaaca cgaataaatc atgcctggca tcagaaaaaa caaaaggaca aatatcggag
     5041 tgatggtaaa aaggccatta atatacatat aagaagcaat gtgaaagaaa tgcttgatga
     5101 gctcagaact gtttacggtc tgagaacagg agagtttttt gaaatgctga ttacagagaa
     5161 atatcttgaa cacaagcgaa ggaagtaatt cattacctgt tacattctaa ataatctcag
     5221 acagatatta tctccgtgaa tcccccgcca cctttccggt gcgcggggtt ttgtcttttt
     5281 tcaccgggaa tacatgtatg aatccgtctg atgccattga ggcaattgaa aaaccgctct
     5341 cctccctgcc ttactcactt tcccgtcaca tcctggaaca tctgcgcaaa ctcaccagtc
     5401 acgaacccgt gattggcatt atgggtaaaa gcggggccgg taaatcttca ctctgtaatg
     5461 cactgtttca gggggaggtc acaccggtca gtgatgttca cgccggcacc cgggaagtgc
     5521 agcgcttccg tctgagtggt cacggtcaca gcatggttat cactgacctg cccggggtgg
     5581 gcgagagccg ggacagggat gcagagtatg aagccctgta ccgtgacatt ctgcctgaac
     5641 tggacctggt gctgtggctg attaaagccg atgaccgggc cctgtctgtg gatgagtatt
     5701 tctggcgaca catcctgcag tgcggacatc agcaggtgct gtttgtggtg acgcaggctg
     5761 acaaaacgga accctgccat gaatgggata tggcaggtat tcagccttct cccgcacagg
     5821 aacagaacat tcgtgagaaa acggaggcgg tattccgtct gttccggccc gtacatccgg
     5881 ttgttgccgt atcggcccgc accggctggg aactggatac gctggtcagt gcgctcatga
     5941 cagcgcttcc cgaccatgcc gccagccccc tgatgacccg actgcaggac gagctgcgca
     6001 cggagtctgt ccgctctcag gcccgtgaac agtttaccgg tgcggtggac cggatatttg
     6061 acacagcgga gagcgtctgt gttgcctctg ttgtccgtac ggccctgcgc gctgttcgtg
     6121 acaccgtggt ctctgttgcc cgcgcggtat ggaactggat cttcttctga acctgttgtg
     6181 gatgatgtcc tccctgcctc tgagtctgct cacaaaagcg ctgttttcgt tactgtctct
     6241 cttgtccgtg caatagactg ataatagaat aaaacgatcg atacctattt taccgatcgt
     6301 taatatcgat cgttatgcta ataataactc ctgttagcaa cgtgcgcaga tacacacaga
     6361 catgagattc agggaacaac agagccacac gtcagaaact tccgtcagcc ggacctccgg
     6421 cactgtaacc ctttacctgc cggtatccac atctgtggat accggctttt ttattcaccc
     6481 tcactctgat taaggaaatg ctgatgaaac gacatctgaa tacctgctac aggctggtat
     6541 ggaatcacat tacgggcgct ttcgtggttg cctccgaact ggcccgcgca cagggtaaac
     6601 gtggcggtgt ggcggttgca ctgtctcttg ccgcggtcac gtcactcccg gtgctggctg
     6661 ctgacatcgt tgtgcacccg ggtgaaacag tgaatggcgg aacactggta aaccatgaca
     6721 accagtttgt atccggaaca gctgatggcg tgactgtcag taccgggctt gagctggggc
     6781 cggacagtga cgaaaacacc ggcgggcaat ggataaaagc gggtggcaca ggcagaaaca
     6841 ccactgtcac cgcaaatggt cgtcagattg tgcaggcagg aggaactgcc agtgatacgg
     6901 ttattcgtga tggcggaggg cagagcctta acggactggc ggtgaacacc acgctggata
     6961 acagaggtga gcagtgggta cacgggggag ggaaagctgc cggtacaatt attaaccagg
     7021 atggttacca gaccataaaa catggcggac tggcaaccgg aaccatcgtc aacaccggtg
     7081 cagaaggtgg tccggagtct gaaaatgtgt ccagcggtca gatggtcgga gggacggctg
     7141 aatccaccac catcaataaa aatggccggc aggttatctg gtcttcgggg atggcacggg
     7201 acaccctcat ttacgccggt ggtgaccaga cggtacacgg agaggcacat aacacccgac
     7261 tggagggggg taaccagtat gtacacaacg gtggcacggc aacagagacg ctgataaacc
     7321 gtgatggctg gcaggtgatt aaggaaggag gaactgccgc gcataccacc atcaaccaga
     7381 aaggaaagct gcaggtgaat gccggcggta aagcgtctga tgtcacccag aacacgggcg
     7441 gtgcactggt taccagtaca gctgcaaccg tcaccggcac aaaccgcctg ggagcattct
     7501 ctgttgtgga gggtaaagct gataatgtcg tactggaaaa tggcggacgc ctggatgtgc
     7561 tgaccggaca cacagccact aatacccgcg tggatgatgg cggaacgctg gatgtccgca
     7621 acggtggcac cgccaccacc gtatccatgg gaaatggcgg tgtactgctg gccgattccg
     7681 gtgccgctgt cagtggtacc cggagcgacg gaaaggcatt cagtatcgga ggcggtcagg
     7741 cggatgccct gatgctggaa aaaggcagtt cattcacgct gaacgccggt gatacggcca
     7801 cggataccac ggtaaatggc ggactgttca ccgccagggg cggcacactg gcgggcacca
     7861 ccacgctgaa taacggcgcc atacttaccc tttccgggaa gacggtgaac aacgataccc
     7921 tgaccatccg tgaaggcgat gcactcctgc agggaggcgc tctcaccggt aacggcagcg
     7981 tggaaaaatc aggaagtggc acactcactg tcagcaacac cacactcacc cagaaagccg
     8041 tcaacctgaa tgaaggcacg ctgacgctga acgacagtac cgtcaccacg gatgtcattg
     8101 ctcagcgcgg tacagccctg aagctgaccg gcagcactgt gctgaacggt gccattgacc
     8161 ccacgaatgt cactctcgcc tctggtgcca cctggaatat tcccgataac gccacggtgc
     8221 agtcggtggt ggatgacctc agccatgccg gacagattca tttcacctcc acccgcacag
     8281 ggaagttcgt accggcaacc ctgaaagtga aaaacctgaa cggacagaat ggcaccatca
     8341 gcctgcgtgt acgcccggat atggcacaga acaatgctga cagactggtc attgacggtg
     8401 gcagggcaac cggaaaaacc atcctgaacc tggtgaacgc cggcaacagt gcgtcggggc
     8461 tggcgaccag cggtaagggt attcaggtgg tggaagccat taacggtgcc accacggagg
     8521 aaggggcctt tatccagggg aataagctgc aggccggtgc ctttaactac tccctcaacc
     8581 gggacagtga tgagagctgg tatctgcgca gtgaaaatgc ttatcgtgca gaagtccccc
     8641 tgtatgcctc catgctgaca caggcaatgg actatgaccg gattctggca ggctcccgca
     8701 gccatcagac cggtgtaagc ggtgaaaata acagcgtccg tctcagcatt cagggcggtc
     8761 atctcggtca cgataacaac ggcggtattg cccgtggagc cacgccggaa agcagcggca
     8821 gctatggctt cgtccgtctg gagggtgacc tgctcagaac agaggttgcc ggtatgtctg
     8881 tgaccgcggg ggtatatggt gctgctggcc attcttccgt tgatgttaag gatgatgacg
     8941 gctcccgtgc cggcacggtc cgggatgatg ccggcagcct gggcggatac ctgaatctga
     9001 tacacaacgc ctccggcctg tgggctgaca ttgtggccca gggaacccgc cacagcatga
     9061 aagcctcatc ggacaataac gacttccgcg tccggggctg gggctggctg ggttcgctgg
     9121 aaaccggtct gcccttcagt atcactgaca atctgatgct ggagccgcaa ctgcagtaca
     9181 cctggcaggg actctccctg gatgacggcc aggataacgc cagttatgtg aagttcgggc
     9241 atggcagtgc acaacatgtg cgtgccggct tccgtctggg cagccaccac gatatgaact
     9301 ttggtaaagg cacctcatcc cgtgacaccc tgcgcggcag tgccaaacac agtgtgcgtg
     9361 aactgccggt gaactggtgg gtacagcctt ctgttatccg caccttcagc tcccgggggg
     9421 acatgagcat gggtacagcc gcagccggca gtaacatgac gttctcaccg tcacagaatg
     9481 gtacgtcact ggacctgcag gccggactgg aagcccgtgt ccgggaaaat atcaccctgg
     9541 gcgttcaggc cagttatgcc cacagcatca acggcagcag cgctgaaggt tataacagtc
     9601 aggccacact gaatgtaacc ttctgataat tctgcattgc ctctctgtgg tcccggtcat
     9661 gatgaccggg acccggacgg gggaaaacgt ttcagtgcca cattcactgg cattcacaat
     9721 aacatgatat tcatcacgga gtgactatgt tacagatagt cggcgcgctg attctgctga
     9781 tcgcaggatt tgccattctt cgccttttgt tcagagcatt aaccagcaca gcgtctgcgc
     9841 tggcagggtt catattgctg tgtctgttcg gtccggcttt actggctggc tatatcactg
     9901 aacgcataac ccggttattc catattcgct ggctggcagg cgtatttctg acgattgccg
     9961 gaatgatcat cagcttcatg tggggacttg atggtaaaca tatcgcactg gaggctcata
    10021 cttttgactc tgtaaaattt attctgacca ccgctctcgc cgctggtctg ctggctcttc
    10081 ccgtgcagat aagaaccatt cagcagaacg ggctcacacc tgaagatatc agcaaggaaa
    10141 ttaacgggta ttactgctgt ttttatactg cttttttcct tatggcgtgt tctgcatacg
    10201 caccattgat cgcattgcag ttcgatattt caccctcact gatgtggtgg ggcgggttgt
    10261 tgtactggct ggctgcatta gtgacgctgc tatgggcggc cagccagatc caggcgctga
    10321 aaagactgac cagtgccatc agccagacac tggaagaaca accggtgctc aacagtaaat
    10381 cgtggctgag cagtttgcaa aacgattaca gccttcctga aacgctgacg gagcgcatct
    10441 ggctgacact catttcacaa cggatttccc ggggagaact gagggaattt gaactggcag
    10501 acggaaactg gttactgaac aatgcctggt atgaaagaaa catggcaggg tttaacgaac
    10561 agttgaaaga gaacctgtca ttcacacctg atgaactgaa aacgctcttc cggaaccgcc
    10621 tgaatttatc accggaagcg aatgacgatt ttctcgatcg ttgcctggac ggcggtgact
    10681 ggtacccctt ttcagaaggc cgccgttttg tatcattcca ccacgtggat gagcttcgta
    10741 tctgtgcctc ctgcgggctg acagaagtac atcatgcccc ggaaaatcat aagccggctc
    10801 cggaatggta ctgctcctct ctttgtcacg aaacagaaac actgtgtcag gacatttatg
    10861 aacgttctta caccggtttt atttccgatg caacggcgaa tggtctgatt ctcatgaaac
    10921 tgccggaaac ctggagtaca aatgagaaaa tgtttgcttc cggagggcag ggacatgggt
    10981 ttgccgctga acggggaaac catattgtcg acagagtccg tctgaaaaac gcacggatcc
    11041 tcggtgataa taatgccaga aatggagcag acagactggt cagcggaaca gaaatccaga
    11101 cgaaatattg ttcaactgca gcccgtagcg tcggtgcggc attcgacgga caaaacggac
    11161 agtatcgtta catgggaaat catggtccca tgcaactgga agtcccccgt gatcagtatg
    11221 ccggcgctgt ggaaaccatg aagaataaga tccgcgaagg taaagtaccc ggtgtaaccg
    11281 atcccgcaga agcgtcccgg ctgattcgtc ggggacatct gacttatacc caggcccgta
    11341 atatcacccg gttcgggacc atcgaatcgg tcacttatga tattgccgag gggtcggttg
    11401 tcagtctggc ggccggaggg atcagttttg ccctgacggc atcggtcttc tggctcagca
    11461 ccggcgatcg cgatgctgcc ctgcagacag ctgctgtcca ggcaggaaaa accttcaccc
    11521 gcacactggc tgtctacgtc acaacccagc aacttcaccg gctcactgtt gttcagggta
    11581 tgctgaagca tattgatttt tcgacggcca gcccgactgt ccggcaggcg cttcagaagg
    11641 ggaccggtgc aggaaatatc agtgccctga acaaagtgat gaagggtacg ctggtgacat
    11701 ctctggcact ggtagctgtc acaaccagcc ctgacatgat caaaatgttg cggggacgga
    11761 tctccggtgc gcagttcatc aggaatcttg ccgtggcatc ttccggtgtg gcaggtggtg
    11821 ctgtcgggtc agtggcgggc gggatattgt tcagtccact gggaccattt ggtgcactga
    11881 cagggcgtgt ggttggcggt gttctggggg gaatgattgc ctccgctgta tcaggaaaaa
    11941 ttgccggagc gctggttgaa gaagatcgcg tcaaaattct ggcaatgatt caggagcagg
    12001 tgacatggct tgccggcagt ttcctgctga ccggacatga gattgaaaat ctgaacgcga
    12061 atctggcccg tgttatcgat cagaatgctc tggagatcat tttcgccgcc ggtatacaac
    12121 aacgggccgc gaccaatatg ttaatcaaac cactggtggt cagtatcatc aggcaacgcc
    12181 ccgtcatgga atatgatgca tcccatctcg gcaagatggt taaccggctg gaagaagcat
    12241 tccccccgga gctcccggca taacgtgtgt tctgctcctt ttaggtttta tcacctgatt
    12301 tgccagtgtc atcgtcatga ctcagagagg taaataccat gattcacctg ttcaaaacct
    12361 gcatgattac cgccttcatt ctggggttaa cgtggtctgc cccactccgg gcacaggatc
    12421 aacgttacat cagtatacgc aatacagata cgatatggct cccgggaaat atttgtgctt
    12481 accagttccg gctggataat ggcggaaacg gtgaaggatt tggccccctc accatcactc
    12541 tgcaactcaa agacaaatat ggtcagacgc tggtgaccag aaaaatggaa acggaagctt
    12601 ttggtgacag taatgccacg cgaaccacag acgcatttct ggaaacggag tgcgtggaaa
    12661 atgtcgccac aaccgaaatc attaaagcaa ctgaagaaag taacggccat cgtgtcagtc
    12721 tgccgttatc ggttttcgat ccccaggact accatccact gctgattacc gtttccggaa
    12781 aaaatgttaa ctgatccctg aagtcagttt catgcttcag cctctgccac aacgtatggc
    12841 agaggctatt tatcaggaga aaccagatat gccgggttac acacagagta tgtcctggct
    12901 gaaggatctt tctcatacgg gcaggcggtt gctgtcataa ccgcttaccg caatgtgttt
    12961 atccaggatg acccgggaat gcatttccgt cgggttatcc gcaacgcgga aggtcagcga
    13021 cgctgacgca gccggaactc tgaagctgat gccggaaaac agcttaatgc gtggctcgcc
    13081 agtggagggc ttctcagaca ataaacttca ttgatttcat ccatcaggcc gcgtcttctc
    13141 cgggagacgc ggcttttttc atttgttgca ccagtaaatc ttaaccaccg ataaggagca
    13201 aagtatgcaa ttagtcagtc gttttggtta tgcgaaccag atacgccgtg accgtccgct
    13261 gacacatgaa gagctgatgc accatgtgcc ggggattttc ggggaagaga aacacacgtc
    13321 ccgaagccag aactatacgt acatccccac catcaccgta ctggaaagtc tgcagcggga
    13381 aggcttccag ccctttttcg cctgccagac ccgtgtgcgc gacccgggcc gccggggata
    13441 cacaaaacac atgctgcgtc tgcggcgggc cggagagata aacggagaac atgtccctga
    13501 aattattctg ctcaactccc atgacggtac ctccagctac cagatgctgc cgggttactt
    13561 cagattcgtc tgccagaacg ggtgtgtctg tggtcagtct ctgggggaag tgcgtgttcc
    13621 gcaccgggga aacgtggtgg agaaagttat tgaaggggct tacgaagtgg tgggcgtgtt
    13681 tgaccggatt gaggagaaac gtgatgccat gcagtcgctg gtcctgccgc caccggcacg
    13741 ccaggcgctg gcacaggcgg cactgactta ccgttatggt gacgaacatc agcccgtcac
    13801 caccgccgac attctgacgc cacgacgccg ggaggattac ggtaaggacc tgtggagtac
    13861 ttatcagacc atccaggaga atatgctgaa aggcgggatt tccggccgca gtgcaaaagg
    13921 aaaacgtatc cacacccgtg ccattcacaa catcgacacc gatattaagc tcaaccgcgc
    13981 attgtgggt
//
LOCUS       DAETQL010000095        13211 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.095, whole genome shotgun sequence.
ACCESSION   DAETQL010000095 DAETQL010000000
VERSION     DAETQL010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.095"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(16..999)
                     /gene="kdgT"
                     /locus_tag="K0W65_003256"
     CDS             complement(16..999)
                     /gene="kdgT"
                     /locus_tag="K0W65_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBI3096403.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="K0W65_003257"
     CDS             complement(1259..1879)
                     /gene="sodA"
                     /locus_tag="K0W65_003257"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBI3096404.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2164..3198
                     /gene="rhaT"
                     /locus_tag="K0W65_003258"
                     /pseudo
     CDS             2164..3198
                     /gene="rhaT"
                     /locus_tag="K0W65_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="K0W65_003259"
     CDS             complement(3195..4061)
                     /gene="rhaR"
                     /locus_tag="K0W65_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HBI3096405.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="K0W65_003260"
     CDS             complement(4117..4953)
                     /gene="rhaS"
                     /locus_tag="K0W65_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HBI3096406.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5241..6710
                     /gene="rhaB"
                     /locus_tag="K0W65_003261"
     CDS             5241..6710
                     /gene="rhaB"
                     /locus_tag="K0W65_003261"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBI3096407.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6707..7925
                     /gene="rhaA"
                     /locus_tag="K0W65_003262"
                     /pseudo
     CDS             6707..7925
                     /gene="rhaA"
                     /locus_tag="K0W65_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
     gene            8167..8991
                     /gene="rhaD"
                     /locus_tag="K0W65_003263"
                     /pseudo
     CDS             8167..8991
                     /gene="rhaD"
                     /locus_tag="K0W65_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9001..9315
                     /gene="rhaM"
                     /locus_tag="K0W65_003264"
     CDS             9001..9315
                     /gene="rhaM"
                     /locus_tag="K0W65_003264"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBI3096408.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9616..10062
                     /locus_tag="K0W65_003265"
     CDS             9616..10062
                     /locus_tag="K0W65_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBI3096409.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10073..11524
                     /locus_tag="K0W65_003266"
     CDS             10073..11524
                     /locus_tag="K0W65_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HBI3096410.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11514..12584
                     /locus_tag="K0W65_003267"
     CDS             11514..12584
                     /locus_tag="K0W65_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBI3096411.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12584..>13211
                     /locus_tag="K0W65_003268"
     CDS             12584..>13211
                     /locus_tag="K0W65_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBI3096412.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQY"
BASE COUNT         3146 a         3452 c         3480 g         3133 t
ORIGIN      
        1 attaaggaag caggtttatt tcaccgtacc taaaatttcg attttcgctg ctctggcttt
       61 gactttacgt gaccagatag aggtaagaat tggcaccaga atcgaagtca caatgaccgc
      121 cgtcgctacc agcgaagttg ctgccggagc catcggttta aacgcaggca ccatttctgc
      181 aatcagcaca ggtgtcgcta ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt
      241 gccatcgcca ccgccaatca atttatcggc gataatcaac ggaataccgg tcacgataat
      301 tactgccaca cccaacagga tccccagcaa accagtctgg gcaatcacag tcaaatcaat
      361 ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa
      421 ttctcgtaat tcagggtcaa gattccccag ggcaaagccc accaggaacg gtaatactgc
      481 gccgacgaaa acatgcggtt caaacgaggc aatcccggca gtgcccagaa taatcatcgt
      541 catgagcggc ccggactcca acgacatcaa cacaaatgcc ccagcttctt ctttcgtacc
      601 gtactgctgc atgatggaag cgtaaagtcc gccgttggtc atatccatcg ccgccaccag
      661 cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca ccatgttccg gaataatgcg
      721 cgaggcaatt gccgcaacca cccacgcgac ggcaatttta gttaccacca gcgtaccgga
      781 tttacgcagt accgttcccg tcgcgcttaa ttttattgac gcccccatgc aaaaaaacca
      841 caccgccaga atgggcaccg taccggtaat catcccgttg gtgaatgatc caaaatattt
      901 ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg aatagcggga cgagcatcat
      961 cccccccggg attttctcaa tcgagcgttt tatctgcatt tccatcacct gtcggcatat
     1021 ttttaagggc tgatgcgtaa ttcctcaatg gagcatcagt gttgatggaa acgaagatag
     1081 acattcattt aatcaataaa agtgatcaaa atcacatcaa ttgaaacgct gttttatttt
     1141 tataggccca ttgtttttat tttggaattg atcacaaaaa aaccaccgcc gttggcgatg
     1201 gttcattttt taagctgata tgcggcctat acgcctcatt gcagcaggcg gcaaatgatt
     1261 attttttcgc cgcaaaacgt gccgctgctt cgtcccagtt caccacgttc cagaactctt
     1321 tgatgtagtc cggacggcgg ttctggaatt tcaggtagta agcatgttcc cacacatcca
     1381 ggcccagaat cgggaagccg gaagcgccag aaatagcttc acccatcagc ggagaatcct
     1441 ggttagcagt agaaaccacc gccagtttat cgcctttcag caccagccat gcccagccgg
     1501 aaccaaagcg ggaagctgcc gctttttcaa attctgcttt gaagttatca acggagccga
     1561 agtcacgttc gatagccgct ttcaggtcac cctgcagggt ggtgcctttt ttcagacctt
     1621 tccagaacag gctgtggtta gcgtgaccgc cagcgttgtt gcgcagtacg gttttcttgt
     1681 ctgctggcag ctggtccagt ttggtgatca gctcttcaac cggcaggttg gcaaattctg
     1741 gcaggctttc cagcgccgcg ttggcgttgt ttacgtaggt ctgatggtgt ttggtgtggt
     1801 ggatttccat ggtctgctta tcgaagtgcg gttccagggc atcgtaagca tacggcaggg
     1861 atggcagggt atagctcata ttcatctcca gtattgtcgg gcggccgatt gttaatgccg
     1921 cgtaagcagt tggttcatta tagttaatta aatgatattg aaaatgatta tcaatgccgt
     1981 acttttcgta agggtatggt tttgcaggaa aatgcccgag atgtgaagca aatcacccac
     2041 ttaatgccgt gattgccagt aaatcgacaa cggcggcaac aggcgaaagg ttaatcgaca
     2101 gcacgatttt tacactcatc tcgtcggaga tgtgaagcga cgaaaaatga tgaggataag
     2161 aagatgagta acgcgattac gatggggata ttttggcatt tgatcggcgc ggccagtgca
     2221 gcctgttttt acgctccgtt caaaaaagta aaaaaatggt catgggaaac catgtggtca
     2281 gtcggtggga ttgtttcgtg gattattctg ccgtgggcca tcagcgccct gttactaccg
     2341 gatttctggg cgtattacag ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc
     2401 ggcgctatgt gggggatcgg taatatcaac tatggcctga ccatgcgtta tctcggcatg
     2461 tcgatgggaa ttggcatcgc cattggcatt acgttgattg tcggtacgct gatgacgcca
     2521 attatcaacg gcaatttcga tgtgctgatt aacaccgaag gcggacgcat gacgttactc
     2581 ggcgttctgg tggcgctgat tggcgtaggg attgtaactc gagccgggca gttgaaagag
     2641 cgcaagatgg gcattaaagc cgaagagttc aatctgaaaa aagggctggt gctggcggtg
     2701 atgtgcggca ttttctctgc cgggatgtcc tttgcgatga acgccgcaaa accgatgcat
     2761 gaagccgctg ccgcacttgg cgtcgatcca ctgtatgttg ctctgccaag ctatgttgtc
     2821 atcatgggcg gcggcgcgat cattaacctc ggtttctgtt ttattcgtct ggcaaaagtg
     2881 aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg
     2941 ttactctcgg cactgggcgg gttgatgtgg tatctgcaat tctttttcta tgcctagggc
     3001 cacgcccgca ttccggcgca gtatgactac atcagttgga tgctgcatat gagtttctat
     3061 gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt ggaacaatac aggacgccgt
     3121 ccggtaacgg tgttgagcct cggttgtgtg gtgattattg tcgccgctaa catcgtcggc
     3181 atcggcatgg cgaattaatc tttctgcgaa ttgagatgac gccactggct gggcgtcatc
     3241 ccggtttccc gggtaaacac caccgaaaaa tagttactat cttcaaagcc acattcggtc
     3301 gaaatatcac tgattaacag gcggctatgc tggagaagat attgcgcatg acacactctg
     3361 acctgccgca gatattgatt gatggtcatt ccagtctgct ggcgaaattg ctgacgcaaa
     3421 acgcgctcac tgcacgatgc ctcatcacaa aatttatcca gcgcaaaggg acttttcagg
     3481 ctagccgcca gccgggtaat cagcttatcc agcaacgttt cgctggatgt tggcggcaac
     3541 gaatcactgg tgtaacgatg gcgattcagc aacatcacca actgcccgaa cagcaactca
     3601 gccatttcgt tagcaaacgg cacatgctga ctactttcat gctcaagctg accgataacc
     3661 tgccgcgcct gcgccatccc cacgctacct aagcgccagt gtggttgccc tgcgctggcg
     3721 ctaaatcccg gaatcgctcc ctgccagtca agattcagct tcagccgctc cgggcaataa
     3781 ataatattct gcaaaaccag atcgttaacg gaagcgtagg agtgtttatc gtcagcatga
     3841 atataaaaga gatcgccacg ggtaatgcga taaggacgat cgttgagtac atgcagacca
     3901 ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg tatgttcggc aaagacatct
     3961 tgcggataac ggtcagccac agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg
     4021 agaagtttta actgatgcgc caccgtggct acctcggcca gagaacgaag ttgattattc
     4081 gcaatatggc gtacaaatac gttgagaaga ttcgcgttat tgcagaaagc catcccgtcc
     4141 ctggcgaata tcacgcggtg accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt
     4201 actgtcgctg aatccacagc gataggcgat gtcagtgacg ctggcctcgc tgtggcgtag
     4261 tagatgtcgg gctttcatca gtcgcaggcg gttcaggtat cgctgaggcg tcagtcccgt
     4321 ttgctgctta agctgccgat gtagcgtacg cagtgaaaga gaaaattgat ccgccacggc
     4381 atcccaattc acctcatcgg caaaatggtc ctccagccag gccagaagca agttgagacg
     4441 tgatgcgctg ttttccaggt tctcctgcaa actgctttta cgcagcaaga gcagtaattg
     4501 cataaacaag atctcgcgac tggcggtcga gggtaaatca ttttcccctt cctgctgttc
     4561 catctgtgca accagctgtc gcacctgctg caatacgctg tggttaacgc gccagtgaga
     4621 cggatactgc ccatccagct cttgtggcag caactgattc agcccggcga gaaactgaaa
     4681 tcgatccggc gagcgataca gcacattggt cagacacaga ttatcggtat gttcatacag
     4741 atgccgatca tgatcgcgta cgaaacagac cgtgccaccg gtgatggtat agggctgccc
     4801 attaaacaca tgaatacccg tgccatgttc gacaatcaca atttcatgaa aatcatgatg
     4861 atgttcagga aaatccgcct gcgggagccg gggttctatc gccacggacg cgttacaaga
     4921 cggaaaaaaa tccacactat gtaatacggt catactggcc tcctgatgtc gtcaacacgg
     4981 cgaaatagta atcacgaggt caggttctta ccttaaattt tcgacggaaa accacgtaaa
     5041 aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa atgcggtgag catcacatca
     5101 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
     5161 attttcctgt cagtaacgag aaggtcgcga attcaggcgc tttttagact ggtcgtaatg
     5221 aaattcagca ggatcacatt atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat
     5281 ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg
     5341 aaatccatcg ttttaacaat gggctgcata gccaaaacgg ttatgtcacc tgggatgtgg
     5401 atagcctgga aagtgccatt cgccttggat taaacaaggt gtgcgaggaa gggattcgta
     5461 tcgatagcat tgggattgat acctggggcg tggactttgt gctgctcgac caacagggtc
     5521 agcgtgtggg cctgcccgtt gcttatcgcg atagccgcac caatggcctg atgccgcagg
     5581 cacaacagca actcggcaaa cgcgatattt atcagcgtag cggcatccag tttctaccct
     5641 tcaatacgct ttatcagttg cgtgcgctga cggagcaaca acctgaactt attccacaca
     5701 ttgctcacgc tctgctgatg ccggattact tcagttatcg cctgaccggc aagatgaact
     5761 gggaatatac caatgccacc accacacaac tggtcaatat caatagcgac gactgggacg
     5821 agtcgctact ggcgtggagc ggggccaaca aagcctggtt tggtcgcccg acgcatccgg
     5881 gtaatgtcat tgggcactgg atttgcccgc agggtaatga gattccggtg gtcgccgttg
     5941 ccagccatga taccgccagc gcggttatcg cctcgccgtt aaacggttca cgcgccgctt
     6001 atctctcttc tggcacctgg tcattgatgg gcttcgaaag ccagacgcca tttaccaatg
     6061 acacggcgct ggcagccaac atcaccaatg aaggcggggc ggaaggtcgc tatcgggtgc
     6121 tgaaaaatat tatgggctta tggctgcttc agcgagtgct tcaggaacgg caaatcaacg
     6181 atctcccggc gcttatcgcc gcgacacagg cacttccggc ctgccgcttc atcatcaatc
     6241 ccaatgacga tcgctttatt aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc
     6301 gggaaacggc gcaaccgatc ccgggaagtg atgctgaact ggcgcgctgt attttcgaca
     6361 gtctggcgct gctgtatgcc gatgtgttgc atgagctggc gcagctgcgc ggtgaagatt
     6421 tctcgcaact gcatattgtc ggcggcggct gccagaacac gctgctcaac cagctatgcg
     6481 ccgatgcctg cggtattcgg gtgatcgccg ggcctgttga agcctcaacg ctcggcaata
     6541 tcggcatcga gttaatgacg ctggatgaac tcaacaatgt ggatgatttc cgtcaggtcg
     6601 tcagcaccac cgcgaatctg accaccttta cccctaatcc tgacagtgaa attgcccact
     6661 atgtggcgca gattcactct acacgacaga caaaggagct ttgcgcatga ccactcaact
     6721 ggaacaggcc tgggagctgg cgaaacagcg tttcgcggcg gtggggattg atgtcgagga
     6781 ggcgctgcgc caacttgatc gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt
     6841 ttccggtttt gaaaacccgg aaggttcgct gaccgggggg attcaggcca caggcaatta
     6901 tccgggcaaa gcgcgtaatg ccagtgagct acgtgccgat ctggaacagg ctatgcggct
     6961 gattccgggg ccgaaacggc ttaatttaca tgccatctat ctggaatcag atacgccagt
     7021 ctcgcgcgac cagatcaaac cagagcactt caaaaactgg gttgaatggg cgaaagccaa
     7081 tcagctcggt ctggatttta acccctcctg cttttcgcat ccgctaagcg ccgatggctt
     7141 tacgctctcg catcccgacg acagcattcg ccagttctgg attgatcact gcaaagccag
     7201 ccgtcgcgtt tcggcctatt ttggcgagca actcggcaca ccatcggtga tgaacatctg
     7261 gatcccggat ggtatgaaag atatcaccgt tgaccgtctc gccccgcgtc agcgtctgct
     7321 ggcagcactg gatgaggtga tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt
     7381 tgagagcaaa ttgtttggca ttggcgcaga gagctacacg gttggctcca atgagtttta
     7441 catggggtat gccaccagcc gccagactgc gctgtgcctg gacgccgggc acttccaccc
     7501 gactgaagtg atttccgaca agatttccgc cgccatgctg tatgtaccgc agttgctgct
     7561 gcacgtcagc cgtccggttc gctgggacag cgatcacgta gtgctgctgg atgatgaaac
     7621 ccaggcaatt gccagtgaga ttgtgcgtca cgatctgttt gaccgggtgc atatcggcct
     7681 tgacttcttc gatgcctcta tcaaccgcat tgccgcgtgc gttgctggaa cctaccgctg
     7741 agctgcgcaa gctggaagcg gcgggcgatt acactgcgcg tctggcactg ctggaagagc
     7801 agaaatcgtt gccgcggcag gcggtctggg aaatgtattg ccaacatcac gatacgccag
     7861 caggtagcga atggctggag agcgtgcggg cttatgagaa agcgattttg agccagcgtg
     7921 ggtaaacact gccggatgcg gcacgagcgc cttatccggc ctacgggtcg aatgtccatg
     7981 cgggctgtca ccctcacccc ggccctctcc ctgaaagggc gagggagaaa aacatagcct
     8041 cgtgcggcaa cggagctgaa caccgctccg gacagtcccc tcgcccctac ggggagaggg
     8101 ttagggtgag gggaacaagc cgcaccatgt ctaaacaagg caacctaatt tacaggaaca
     8161 cagaacatgc aaaacattac ccagtcctgg tttgtccagg gaatgatcaa agccaccacc
     8221 gatgcctagc tgaaaggctg ggacgagcgc aacggcggca acctgacgct gcgcctggat
     8281 gacgccgata tcgcgcttta tcacgacaac ttccacccac aaccgcgcta tattccgctc
     8341 agccaaccca tgcctttact ggcaaatacg ccgtttattg tcaccggctc cggcaaattc
     8401 ttccgtaacg tccagctcga tcctgcagct aacttaggcg tcgtaaaagt cgacagcgat
     8461 ggcgcgggct accatattct ctgggggtta accaacgaag ccgttccaac ttctgaactt
     8521 ccggctcact ttctctccca ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg
     8581 atcatgcact gccacgccac caacctgatc gccctcacct atgtactgga aaacgacacc
     8641 gcggtcttta ctcgccaact gtgggaaggc agcaccgaat gtctggtagt attcccggat
     8701 ggcgttggca ttctgccgtg gatggtgccc ggcacggacg aaatcggcca ggcgaccgca
     8761 caagagatgc aaaaacattc gctggtgttg tggcccttcc acggtgtgtt cggttccgga
     8821 ccgacgctgg atgaagcctt tggtttaatc gacaccgcag aaaaatcagc acaagtatta
     8881 gtgaaggttt attcgatggg cggcatgaaa cagaccatca gccgtgaaga gttgatagcg
     8941 ctcggcaagc gcttcggcgt tacaccacta gccagtgcgc tggcgctgta aggagcaaac
     9001 atgatccgca aagcctttgt catgcaggta aaccccgacg cccacgaaga gtatcagcgt
     9061 cggcataatc ccatctggcc ggaactggaa gcagtgttga aatctcacgg tgtgcataac
     9121 tacgccattt acctcgacaa agcgcgtaat ctgctgtttg ccatggtaga gattgaatct
     9181 gaagaacggt ggaatgcggt agccagtact gatgtttgcc aacgttggtg gaaatatatg
     9241 accgatgtta tgcccgctaa cccagataac agcccggtga gtagcgagct gcaagaagtg
     9301 ttttacctgc cgtaattgcc caatgtgccg gagatgcctc cggcacaata ttttaaattt
     9361 cgatttgaaa aattaaacat attacatctc ccggtaagtt atatttccct gatacattgt
     9421 gagtaaatca caaaaataat gaataaccca ttaatgattc atgtggttta tttaaataac
     9481 cattatgtgc attactccgc aaatctgacc tttcactctg tctcaaattg tcattgttta
     9541 ccgacatatc gcctctcatc acttaatcgc tccaccatta caattgatta aaatatattt
     9601 tatggagtcc gattaatggc agctcttact gcaagctgta ttgacctgaa tattcagggc
     9661 aatggcgctt attccgttct gaagcagttg gcgacaatag cgttacaaaa cggttttatc
     9721 accgactcac accagttcct gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga
     9781 tttggttccg gtgtcgcagt gccgcacggt aaaagcgcct gcgttaaaca accgttcgta
     9841 ttattcgccc gcaaagcgca ggctattgac tggaaagcca gcgatggcga agacgtcaat
     9901 tgctggatct gcctcggcgt gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc
     9961 acactgtgtc gcaaaattat tcaccaggaa tttattcatc aactgcaaca gggcgatacc
    10021 gaccaggtgc tggccttgtt aaatcaaacc ctcagctcat aaggaagtgg cgatggagtc
    10081 atccttacgt attgtcgcga tcaccaactg ccccgccggg atcgctcaca cctacatggt
    10141 ggcggaagcc ctggaacaga aagcccgttc tctcggccat accataaaag tggaaactca
    10201 agggtccagt ggcgttgaaa accgcttatc cagcgaagag attgccgctg ccgattacgt
    10261 cattcttgct accgggcgtg gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa
    10321 agtttatgag attgccatct ccctggcgtt gaaaaatatc gaccagattt tcagcgaatt
    10381 accgacaaac tcgcagcttt ttgccgcaga tagcggcgtg aagcttggca aacaggaagt
    10441 gcaaagcggc agcgtgatga gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt
    10501 catcggcggt ggtatcctgg tggcgcttgc caacatgctg gtgcagttcg gtttgccgta
    10561 taccgatatg tcgaaaggcg cgccgtcgtt tacctgggta gttgaatcca tcggttacct
    10621 tggcttcacc tttatgatcc ccatcatggg cgcttatatt gcctcgtcga ttgccgataa
    10681 accggctttc gctccggcgt ttctggtttg ctatctggcg aacgacaaag cgctgctcgg
    10741 cacccagtcg ggtgcgggat tcctcggcgc ggtggtgctg gggctggcga ttggctattt
    10801 cgtcttctgg ttccgcaaag tgcgccttgg caaagcatta caaccgctgc tcggttcaat
    10861 gctgatcccg ttcgttactc tgctggtttt cggcgtgctc acttactacg ttatcgggcc
    10921 ggtgatgtcc gacctcatgg gcgggctgct ccacttcctg aataccattc ctccatcaat
    10981 gaagtttgcg gcagcgtttc tggtcggtgc gatgctggcg ttcgatatgg gcggccccat
    11041 caataaaacc gcctggttct tctgcttctc actgctggaa aaacacattt atgactggta
    11101 cgccatcgtc ggcgttgtcg cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat
    11161 tgcgcccaaa ctgtttaccc gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg
    11221 tgccacagtc gcaaccgaac cggctatacc ttacgctctg gcagctccgt tgccgatgat
    11281 taccgccaat acgctggcgg gcggcattac cggcgtgctg gtgatcgctt tcggaatcaa
    11341 acgtctggca cctggtttgg gtatctttga cccgctgatc ggcctgatgt cgccggtagg
    11401 ttcgttttat ctggtgctgg cgattggcct cgcgctgaac atttcattca ttatcgtcct
    11461 gaaaggattg tggttacgcc gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca
    11521 ttgagttact gcaacagttg tgcgaagcca gcgccgttag cggcgatgaa caggaagttc
    11581 gcgacatcct gataaacacg ctggaacctt gcgttaatga aatcaccttt gatggtctgg
    11641 gcagctttgt tgcccgtaag ggaaataaag gtccaaaagt tgccgttgtc gggcatatgg
    11701 atgaagtcgg ctttatggtc acccacatcg acgagagcgg ttttctgcgt ttcaccacca
    11761 ttggtggctg gtggaatcag tcgatgctca accatcgggt aaccatacgc acacacaagg
    11821 gagtgaaaat ccctggtgtg attggtttcg tcgcgcctca tgcgttaacg gaaaagcaaa
    11881 ggcaacagcc gctgtcattt gatgagatgt tcattgatat tggcgcaaac agtcgcgatg
    11941 aggtggaaaa acgcggtgtg gcgatgggcg attttattag cccggaagcc aattttgcct
    12001 gctggggcga agataaagtg gtcggcaagg cgctggataa ccgcatcggc tgcgcaatga
    12061 tggctgaact attgcagacg gtgaataatc ccgaaattac gctgtatggc gtcggcagcg
    12121 tggaagaaga agttgggcta cgcggggcac aaacctcggc ggaacacatt aaaccggatg
    12181 tagtgatcgt gctggatacc gctgtcgcag gcgatgttcc gggcattgat aacattaaat
    12241 acccgctgaa actgggccag gggccggggc tgatgctgtt tgacaagcgc tacttcccca
    12301 accagaaact ggtagcagcg ttaaaaaact gtgccgcaca taacgattta ccgctgcaat
    12361 tttccaccat gaaaaccgga gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc
    12421 gtccggttgt tgcgctgtgc ctgccaaccc gctatctgca cgctaacagc ggcatgattt
    12481 caaaagccga ttacgatgct ctgctcacgt tgatacgggg ttttctgacg accttaaccg
    12541 cggagaaagt caacgcgttt agccagttcc gtcaggtgga ttaatgctaa acgaacgcca
    12601 gttaaagatt gtcgatctgc tggagcaaca gccgcgcacg cctggtgagc tggcgcaaca
    12661 gactggcgtt tcaggcagga ccatcctgcg tgatattgac tatctcaact ttacccttaa
    12721 cggcaaagcc cgcattttcg ccagtggcag tgcgggctat cagctggaaa tcttcgagcg
    12781 ccgcagcttt tttcagttgc tgcaaaagca cgataacgac gatcggctgc tggcgctgtt
    12841 attactgaat actttcactc cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac
    12901 gtgggtagca gagcgtctgc cccagttaaa acagcgttat gaacgcactt gttgcctggc
    12961 cagccgccct ggtttgggcc atttcattga tgagacagaa gagaaactcg ttatcttgct
    13021 ggcgaacttg ctgcgcaaag atccgttttt aattccgctg gcgggcataa cacgagacaa
    13081 ccttcagcat ttatccacgg cctgcgacaa ccaacaccgc tggccgctca tacagggtga
    13141 ttatctctcc agcctgattc tggcgattta cgccctgcgt aatcaactga ccgatgagtg
    13201 gccgcaatat c
//
LOCUS       DAETQL010000096        13104 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.096, whole genome shotgun sequence.
ACCESSION   DAETQL010000096 DAETQL010000000
VERSION     DAETQL010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13104
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.096"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..896)
                     /locus_tag="K0W65_003269"
     CDS             complement(<1..896)
                     /locus_tag="K0W65_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3096413.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGG"
     gene            1137..1937
                     /locus_tag="K0W65_003270"
     CDS             1137..1937
                     /locus_tag="K0W65_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBI3096414.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2015..2785
                     /locus_tag="K0W65_003271"
     CDS             2015..2785
                     /locus_tag="K0W65_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBI3096415.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2833..4179)
                     /gene="mltD"
                     /locus_tag="K0W65_003272"
     CDS             complement(2833..4179)
                     /gene="mltD"
                     /locus_tag="K0W65_003272"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBI3096416.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4251..5006)
                     /gene="gloB"
                     /locus_tag="K0W65_003273"
     CDS             complement(4251..5006)
                     /gene="gloB"
                     /locus_tag="K0W65_003273"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBI3096417.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5040..5762
                     /locus_tag="K0W65_003274"
     CDS             5040..5762
                     /locus_tag="K0W65_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBI3096418.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5759..6226)
                     /gene="rnhA"
                     /locus_tag="K0W65_003275"
     CDS             complement(5759..6226)
                     /gene="rnhA"
                     /locus_tag="K0W65_003275"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBI3096419.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6291..7022
                     /gene="dnaQ"
                     /locus_tag="K0W65_003276"
     CDS             6291..7022
                     /gene="dnaQ"
                     /locus_tag="K0W65_003276"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBI3096420.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7155..7231
                     /locus_tag="K0W65_003277"
     tRNA            7155..7231
                     /locus_tag="K0W65_003277"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7189..7191,aa:Asp,seq:gtc)
     gene            complement(7362..8408)
                     /locus_tag="K0W65_003278"
     CDS             complement(7362..8408)
                     /locus_tag="K0W65_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HBI3096421.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8422..8981)
                     /gene="tssG"
                     /locus_tag="K0W65_003279"
                     /pseudo
     CDS             complement(8422..8981)
                     /gene="tssG"
                     /locus_tag="K0W65_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8978..10861)
                     /gene="tssF"
                     /locus_tag="K0W65_003280"
     CDS             complement(8978..10861)
                     /gene="tssF"
                     /locus_tag="K0W65_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBI3096422.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10877..11371)
                     /gene="tssE"
                     /locus_tag="K0W65_003281"
     CDS             complement(10877..11371)
                     /gene="tssE"
                     /locus_tag="K0W65_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBI3096423.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11368..12198)
                     /locus_tag="K0W65_003282"
                     /pseudo
     CDS             complement(11368..12198)
                     /locus_tag="K0W65_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12185..13099)
                     /gene="tagK"
                     /locus_tag="K0W65_003283"
                     /pseudo
     CDS             complement(12185..13099)
                     /gene="tagK"
                     /locus_tag="K0W65_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3007 a         3397 c         3485 g         3215 t
ORIGIN      
        1 cctccgggag ctgtttttac atgctcgcta aggaaatcga taaaagcccg gatgcgcgta
       61 cttaccgcac ggtcgctgta atagacggca ctgaatggca tttccactgg caacacttta
      121 tctgccatta actccaccaa ttctccgcga gcgatttctt tgtcgatcat gtagtcggac
      181 aaacacgcaa tcccgttgcc actcaggcaa agctgtttca gtgtttcccc actattggat
      241 gacaaaccgt acttcacctc atgtaattgt ccatcactac aggctatcgg ccaggtattg
      301 agggaagcgg gttcagtgaa tcccaggcaa acatgttgct ttaaatcgtc gatcgtttct
      361 ggcttcccgt agcgggaaat ataatcgggg gaggcgataa tttttcgata actgttaaat
      421 aacggcctgg cacgtaagct ggagtccgtt aacgtaccag cgcgtatcgc gacatccact
      481 tttctttcga tcaaattaat aatcgtttcg gaggagacta gcgataaagt gacttccgga
      541 tagcgttcac ggaaaggctt aattaacggc atcagaaagt gcagcaccac tggagttgcg
      601 gcatcgatcc gtaacagtcc acgcggtgta ttacgcgtct ccataatttc tgattctgcc
      661 gctgccatct cctgcaaaat tgactgtacg cgacgaaaat aacgctcgcc ttcttccgtc
      721 aggctaagtt gccgcgtggt ccgattaagc aggctaacgc caagtttcat ttccagcttt
      781 ttcaccgccc ggcttaccgc tgagtttgct tgccctaatt gttccgctgc ccggctaaag
      841 ctgccgcttt cgacgaccga aacaaaaatg gcgagttctt ccgacgtggc tttcattttt
      901 gctcctgttg caaaatagat gagatatttt gaatttattt gtcattaaac catcaggatg
      961 cgtgatatgt catccgattt aatgttctca ataatgagca aatttctgac aggtgtaagc
     1021 acttgcttac ataacaatat acaattgctc gttgaaagag tgagctaaaa tccctataac
     1081 agtagaaccc tcccgagtgc ggaagggttg acgtaataga ggtttcaaag tcaaaagtgc
     1141 gaaaaaacac ctatgccatg cgctatgttg ccggacaacc tgcggaaagg atcttaccgc
     1201 cggggtcttt tgcgagcatc ggccaggcat taccacctgg agaaccgtta agtaccgaag
     1261 agcgtattcg gatcctggtg tggaacatat acaaacagca acgcgctgaa tggttgtcgg
     1321 tattaaagaa ctacggcaaa gatgcacatc tggtgttatt gcaggaagcg cagacaacgc
     1381 cagagttagt acagtttgcg accgctaact atcttgccgc cgatcaggtg cccgctttcg
     1441 tgctgccaca acatccttcc ggtgtaatga ccctttcggc ggcgcatcca gtgtattgct
     1501 gcccgttacg tgaacgagaa cccattttgc gtctggcgaa gtcggcactg gtgacggtct
     1561 atccattacc tgacacccgc ctgttgatgg tggttaatat acacgccgtc aacttcagtc
     1621 tgggcgtgga tgtctatagt aagcagttac ttcctattgg tgatcagata gctcaccaca
     1681 gcggcccggt cattatggcg ggagatttca atgcctggag ccgtagaagg atgaacgcgt
     1741 tatatcgctt tgcgcgggaa atgtcgctgc gccaggtgcg ttttaccgat gatcagcgcc
     1801 gtcgggcgtt tggccgcccg ctcgattttg ttttctaccg tggtctgaac gtcagcgaag
     1861 cttctgtatt ggttacgcgc gcttccgatc acaatccgct actcgttgaa ttcagtcccg
     1921 gcaagcctga taaataaggt atgtcaggtc tgccacaggg cagaccaacg tttggcgctg
     1981 tccttttata ctcaaacaac aaaaggacag cgcaatgaca acacaatccc accatgacca
     2041 cgtagaaaag cagtttagct ctcaagcctg cgaatatctg accagtaccg tacatgcatc
     2101 cggacgagat ttacagcgcc tggcggtgcg tctggctgat tatcctggtg caagtgcact
     2161 tgatatgggc tgcggagcag ggcatgccag ctttgttgct gcgcaaaacg tgagcgcggt
     2221 ggtggcgtat gacttatctg cccaaatgct ggatgtcgtg gcacaagctg ccgaagcccg
     2281 gcaactgaaa aatatcacca cccgccaggg atatgccgaa agtctgccat ttgccgataa
     2341 cgcatttgat attgttatca gccgttattc tgcccatcac tggcatgatg ttggtgcagc
     2401 actgcgtgaa gtgaaccgga tattgaaacc tggcggtagg atgattgtga tggacgtaat
     2461 gtctccgggt catccagtgc gcgacatctg gttacagacg gtagaagcat tacgcgatac
     2521 ctctcacgta cgaaactacg ccagcggtga gtggttgagg ttaatcaatg aagccaatct
     2581 gatagttgat aatttaatta cagataagtt accgctggaa ttttgttcat gggtcgcgag
     2641 aatgcgtacg ccagaagcgt tagtagacgc tattcgcatt taccaacaga gcgcatcgac
     2701 agaggtgaga acgtattttg ccttgcagaa tgacggctct ttcaccagtg atatcatcat
     2761 ggtagaagca cataaagcgg cataaataaa aaaggcaccg ggggaatcgg taccttttta
     2821 ttatctggtt tgtcaggaat ctggcatgtt gttgtttttc acaaacaacg tcagcttgtc
     2881 gcctggttgc agattcgcag tgtcgctgtt ccagcgcatc acatctttga tgttcacgcc
     2941 gtggcgttta gcaatgcttg aaagcgaatc gcctttgcgc acacgatacg taatgctatc
     3001 gctgttgttt gccagtcgct gtgcaccaat cgtcaaactt tggcctggct tcagcttaga
     3061 tccgcgcagt ttgttccact gctgcaaatc tttggtgctt acgccgagac gtgaagcgat
     3121 gcttgaaagc gtgtcgccag agcgtacggt gtaaacacgg ctgttaagcg gcgtattgtt
     3181 ggcaaccagc gtcgactgta cagcagcaat ttcgcctgaa gcgagagatt cacgcagttg
     3241 atctgcatgc ttctttggca ccatcacgta ctgcggccca cttgcgccca gcgtggagcc
     3301 tttcacgcca gcgttgaatg tcttcagctt gctgacggaa atccccgcca tatctgcaac
     3361 cttcgccatt tcaaccgggc tactcaggtg cacacgcgcc agagcacggc tttcatcggt
     3421 cgttggcaga cgtacgccat aacgcttgct gtttttgaga atatcactca atgccagcat
     3481 tttaggcacg tactgcttcg tttcctgcgg caacggtaac gaccagaagt ccgtggattt
     3541 cccacgcgct ttgttcgttt taattgcctt catgacccga ccttcgccgc tgttataagc
     3601 cgctacggtc agaagccagt cgtcgtcaaa cattttgttc agacgctgca tcatattcag
     3661 cgcggcagtt gttgaagcaa caacatcgcg acgcgcgtca taattgcggg tctgtttcaa
     3721 accataattg cgccccgtgc tcggaatgat ctgccagatg cctgcggcat tggcgccaga
     3781 cgttgcgtga ggatcaaaag cgctctccac tatgggtagt agtaccagtt ccataggcat
     3841 gttacgtttt ttaacttgcc ctgctatcca gtacatatac ggctctgccc gtaaagttac
     3901 atcgtggaga tagctcttat tgcgtaaata tttctgtttc tgttcgcgaa tccggtcatt
     3961 ttccggaatt cccatcttta gctcgtcgcc aatgaaagcc cacaagtcac catctggcgc
     4021 gatagacgtc ccatcgtcca tccatcgtgc ctgacttgta aactttgctg cttccccttg
     4081 accagctgca gaaaggctct gtgcgtgctg ttgaacgttg ccggtactct ggcaacccac
     4141 gagcaggaca gaggcgagta atatcgcttt tgccttcatg tgtgtgtcaa tagttgctta
     4201 aaagacgacc gatcataacg gcgaacggag ccgatgacaa gaaagtttta tcagaaccta
     4261 tctttctttg accttaacca tgcaaaacgc tcttcaggtt gttgcaataa tgtttcttca
     4321 ttaattccat taattaaatc aatatcttcc gttcttaaaa aaacattaat ttgccgctca
     4381 tttttcagaa ttacgggtag tgttatttga ttttttgccc gtaactcctt aactttacga
     4441 taataatcat ttatggacaa atcgtgcgga aaaatactca aagcaaactt catatttgat
     4501 aacgtatatt catgagcaca acataccaat gtatcgtcag gtaacgcact tagcttttta
     4561 agtgattgat acatttgtaa agctgtccct tcaaacaacc gaccacaccc accagaaaac
     4621 agcgtgtcgc cgcaaaatag ataaggttta ctgaagtaac agatatgtcc taaagtgtga
     4681 cccggtgtgg taattacact aaattcatgc cctaaaacga aggcagtttc gccatctttg
     4741 actacctgtg ttgttccctt atcttgtgtc tcttgtggac catacactac aatttgcgga
     4801 aacttttcca ccagttcttt tacgccgcca acgtgatcgt ggtgatggtg ggttagaaat
     4861 atggcctccg gttgccagtt attggcggta atggcgttta atactggctc tgcgtctccg
     4921 ggatcgacaa tcaggcagcg acctgcctca tcattcaaaa cccagatgta attgtcatca
     4981 aaggcgggaa tactgttaag attcatagat tacctctcag tgtaaaacgg aaggttgtga
     5041 tgaaaccggc aagagtccct caaactgtcg tggctcctga ttgctggggc gatttgccct
     5101 ggggagagct ttatcgcaag gcgctggagc gccagctcaa cccgtggttc actaaaatgt
     5161 atggttttca tctgcttaag attggcaatt taagcgcaga aatcaattgc gaagcgtgcg
     5221 cggtttctca tcaagtgaat gtttctgcgc aaggaatgcc cgtccaggtg caggcggatc
     5281 cacttcatct tccttttgcc gataaatccg ttgatatttg tctactggca cataccttgc
     5341 cgtggtgcac cgatccgcat cgtttattac gtgaagccga tcgggtactg attgatgatg
     5401 gctggctggt gattagtggc ttcaacccca tcagtttaat gggattacgc aaacttgtgc
     5461 cggtattgcg caaaacctcg ccctataata gccggatgtt tactctgatg cggcaactgg
     5521 actggctctc tttgttgaat tttgaagtgc tgcacgccag ccgtttccac gttctcccgt
     5581 ggaacaaaca cggaggaaaa ctattgaatg cgcatattcc tgcgcttggt tgcttacaac
     5641 ttattgttgc ccggaaacgg actattcctt taacgctaaa tccgatgaaa cagagtaaaa
     5701 acaagccacg aattcgccag gcggttggag ccacccggca atgtcgtaaa ccacaggctt
     5761 aaacttcgac ttggtagccc gtatcttcca gtgtgggatt catcgccgcg gcacgagcca
     5821 gttcatcaca gcgttcgttt tccgggtgtc cggcatggcc tttaacccat tcccatttga
     5881 tttgatgctg ccccaatgca gcatcaagac gttgccagag atcgacattt tttactggtt
     5941 ttttgtctgc tgttttccag ccacgttttt tccagttatg gatccactgg gtgataccct
     6001 ggcggacata ctggctgtcg gtactcaaaa tgacttcgca atgttctttt aacgcctcca
     6061 gcgcgacaat agcggccatc aactccatac ggttgttggt ggtgcgggtg tagcctgcgc
     6121 taaaggtttt ctcgcgtccg cgatagcgta aaatagcgcc gtaaccccca ggtccaggat
     6181 tacccagaca cgaaccatcg gtgaaaattt ctacctgttt aagcatctct ggtagacttc
     6241 ctgtaattga atcgaactgt aaaacgacaa gtctgacata aatgaccgct atgagcactg
     6301 caattacacg ccagatcgtt ctcgataccg aaaccaccgg tatgaaccag attggtgcac
     6361 actatgaagg ccacaagatc attgagattg gtgctgttga agtagtgaac cgtcgcctga
     6421 cgggcaataa cttccatgtt tatctcaaac ccgatcggct ggtggatccg gaagcctttg
     6481 gcgtacatgg tattgctgat gaatttttgc tcgataagcc cacgtttgcc gaagtagccg
     6541 atgagtttat ggactatatc cgcggcgcgg agttggtgat ccataacgca gcgttcgata
     6601 taggctttat ggactacgag ttttcgttgc ttaagcgcga tattccgaag accaatacct
     6661 tctgtaaggt caccgatagc cttgcggtgg cgaggaaaat gtttcccggt aaacgcaaca
     6721 gcctcgatgc gctatgtgcc cgctacgaaa tagataacag taaacgaacg ctgcacgggg
     6781 cattactcga tgcccagatc cttgcggaag tttatctggc gatgaccggt ggtcaaacgt
     6841 cgatggcttt tgcgatggaa ggagagacac agcagcaaca aggtgaagca acaattcagc
     6901 gcattgtacg tcaggcaagt aagttacgcg ttgtttttgc gacagatgaa gagctggcag
     6961 cacatgaagc ccgtctcgat ctggtgcaga aaaaaggcgg aagctgcctc tggcgggcat
     7021 aaatacatgt aaaaggcgct aaaaatagcg acttgggcga tttttgcagc aaacgattca
     7081 aaagatgaga aaaatcgttg acgaaggtcg aggcaatccg taatattcgc ctcgttccca
     7141 acggaacaca acgcggagcg gtagttcagt cggttagaat acctgcctgt cacgcagggg
     7201 gtcgcgggtt cgagtcccgt ccgttccgcc aatattcaga aagcccgaat cagtaatgat
     7261 tcgggctttc gtcgttttgg tgaagtgaga atttttcact ccgaagtaaa acggatatga
     7321 tttaaaaaat ggccagacat tataaattct ggccacttct gttactccag cgcatcccat
     7381 ttctccagca gttctactgg aatttcattt attacctccg agacgcgttt tccgaccagg
     7441 cgttctgcct ggcgcagcaa aggaatggtc gggctgctcg gttcgctgtg ctcaaaccag
     7501 cgacggatga cacgcaggcg ttccagcgcg tcgtttcggt cgcggatctg ccggggttct
     7561 gcgccggtca tcggtgatat ttcctccgcg atatgcaccg gtagcgcaat ctgctctgcc
     7621 tgcatctgtt cagtatgaac gatggtggcg ttttccggct gctcagcctg tggttgtggc
     7681 aggatctccg gcgtggtgct ctgcactgct cccggtaaca gggccagcag ttgcgtcaga
     7741 cgggagaagt ccggtgcctg gtcattcagc gtttcacgcg catggcgttg cagccgctca
     7801 gtggtctctg ctgcctggcg aaaggcatcc agcggcagtg tgccgcgcgc ttccaggtct
     7861 gccagttgct ggcgtaccga ttctggtgcc agcgcatccg ccgggcgtaa tgcagatagc
     7921 gaacgctcca catcccgcac ctgaaggcgc atggcagcat tatttgacag tgtaatgcca
     7981 cgaatatccg ccattacacc ttcgtggtcc agcaacgccg ccagcgcgtt actgcgtgcc
     8041 accgcagcat cttcggcggt gatatcttca gtcgcgagca gttgaggatg gatggcgtcc
     8101 gggtacgtgg agcacaacgt ttccagcagc gtgagcgcct ccgacagtcc cctggcaccg
     8161 gcctgctgga tacggcagcg cagcaggaca accagaatgc ggatatcttt gctgcgcatc
     8221 agcagacgat gggcgtcccg ctcaagctcc gcccagttaa tgttttccgg cgtactgaca
     8281 aaatcgccat attgcgcttc ttcacgcggt gcggcgcggg tgaacagcag caggtattcc
     8341 ggatcgtact ccagatccgg gccgcagggg cattccgccg aaattgggtc agacaggaat
     8401 atttccattt aattatcctc agtgcgccag cgtggtggcc agaccgtccg gattgctgga
     8461 ctcacgcacc agatgggccg atgccgccag acgagcgtga tctggcaacg ggctgtcagc
     8521 gctctcccgc aaatcctgcc cgctgaggct ggcgacataa aaggcaaagc ggtcatgttg
     8581 cggtttatcc agaccgcccc cagcggactg ggcgctgcgc catgcctggt aaaactgcgt
     8641 cagccagcgg tgatgaaaaa gatcagaaaa acgggtcagc gtcggctttc agtccggtta
     8701 cgcgccagtt cggtgtagtg cagcggcagt ggaccgttcg gcccccacag cacgaggctg
     8761 tacagactga tgtgcaggcg cccttcctgc cagcccacac tggcgatttc tcgtggcgca
     8821 aaggtcagcg cgggcgtttg tccgaggcga aatttttcca tccgtggttg ccaggtgttg
     8881 cccgccggaa cggtacacaa ctgcacatcc acgcggcgca tcaggttcag aaaaccgtaa
     8941 cgccaggggg gggcctgcgc ctgagtgaaa tcgttgctca tacgcttccc cgctgtcccg
     9001 ccctgacagg ccaggtcata attttgccgc gctgcatgct gtgcagcgtc atctgcgaga
     9061 aggtattaat ggaaacatgg cgggcgatat agtgttccag caccaggccg aacagatacg
     9121 gactgatgcc ggaaaaacct tcttcatcca ccgtcagttc acaactgacg ccacgcccat
     9181 agaccagtag tccggaaccg ggcagacggc gggtgaccgg ggtcgtttta caaccaatca
     9241 ggctgcgcac ctggcgcgat tgcggactgt catgagcagg aataaacagg ttcagcagat
     9301 ctcgcaacgc ctggccgccg gtacggtgat ccagatcggc cagcggcaga taattaaaag
     9361 ataactggcg gatcaaccgc catgccatct cacgttcagc catcgggggc tgcggcgagc
     9421 gtggcgggcg gataagccct acgcccgcca ccgggatggc tgcatccacg gttagatcgt
     9481 cccggccatt gcgggcaata agacagggca gatcgcggtt agtgaccatg gccgtgatgg
     9541 tgatgtggcg cagattttcc gggtacggcg cttcatactg gtcaaccagc gacaggaaca
     9601 cttccgagcc cgtatagggg gtacgggtgc cgtaacggcg ggcgttttct gataagcggc
     9661 ggggctcccg gcgcagggaa aaataacgtc cgtgattgcc ttcatcatta ttgcgggtgt
     9721 ggtagagcgg gcgaaaggcc atcttccgtg tggtgtcggt ttccagccct tccacttcct
     9781 ggaccgagaa cacctcataa tccagcggat gggttctgtc caccaccaga tgctgttccg
     9841 tcgtgctgtg cgtgacatcg atccgggcgg tgacgcgggg aaacaggttg ataaccgggg
     9901 tacagaacag gctgaactgt gccgcatttg tctggtggat cagccagtcc ggcggcaggc
     9961 ggttcagcag gataacaatc tccgccacgc caccgtcaat tttctgtaat ccggcagaca
    10021 ggcctgtcgg ggtgaaaaaa tagaagcgtt ccggacaggc aaaatattca tgcagcaggt
    10081 tatgaccgtg aaacacgttc cacgccagcg gcagcagccc ctgatccggc tccaggcctt
    10141 cgtacataac gggctgttgc agattcacat ccagcgcgcc gtcaaaatgc cccggaatgc
    10201 cagccagggg cgcgatggcg ctggtatgta gaagttctaa caggtgtgag gccgtacgct
    10261 cctcgccgca gagataaaac ggcagcctgt ccagtccggc aagctgactg aacgttagtt
    10321 caccaaaggt gcgcagggta atacgcagcg ccccggcgac gtgaatgttg gccggcaggt
    10381 agcggtgcag ggcaggcata tccggtggcg cggcggtcag tcgcgcctct tcgatggcga
    10441 gcggccacag cgttacatcc tggctgctgc gaaactggca ggcagtgctt tcgccttccg
    10501 gaatggcgga gaaaaaggct gtgtcgcgcg ggacggtaaa tcctttcgcc agatcgcctt
    10561 cttccgtatc gggatgcaac tgcgccacgc tcattgaggg cgtgggtgtg acgtaattgg
    10621 ggctgaccac ttccagcagg cgctgggtga aacgggggaa ttcagcgtca atttttagct
    10681 gcgtgcgggc ggtcaggaag ctgaaggcct cgaccattcg ctctacatac ggatcggcaa
    10741 tatccgttcc ctgcatgccc agccgggcgg cgactttggg atgacgggcg gcaaattcgg
    10801 ctcccgtttc ccgcagatag ctcagttcgc ggttgtagta ttccagtagc cgtggatcca
    10861 tcggtctctc tctgaattaa aaaaacgtaa tgcgactctg ctccatatcc agcgcgctac
    10921 gtacccggaa tgcagtcgga tacggctgcg tgaggatctc gccgcgaatt tcaaactgta
    10981 atgtgttatg gccactctgg cggcgctcat cctgaagtgg gatgatttgc agagtggacg
    11041 ctttcagccg gggttcaaac cgggtgatgg ttcgcaaaat ggccctgcgg atatcgtccc
    11101 acttgtgctc atacagaaag ctgcccgcca gtggcggcag gccgtagtta agtacggacg
    11161 ctgccgcgta cggataacgt gcagcatcaa tatcatcttc atggctgacg gtgttgagca
    11221 gaaatgacaa atcccggcgg atgatctcct taatctgaac cggactgacg ctgataccgt
    11281 ggtcgcgttt ctggtttggg gcgtcgtcac acaggcggtc aaacagcgtg ggcagcaggt
    11341 gattgcctgg acgctggcgg gaagcgctca cgcgtcgctt ccttcagcgg tgttaaaggt
    11401 acaggtggac aaatccagca ggctgatatc gccgtgaccg gtgagccaca ccctctgacc
    11461 cagcgcctgt accagtgtct ctcccggacc gtcctgccag accgtttccc ggcacagacg
    11521 cagggagtcg gcagcctttt cagagccgct gtagcgggga aacagctagg cactgtgtgt
    11581 atcgccattg ttcagggtga cgttgacggg tttccagatc aaatcgatca gatgcgcagg
    11641 ccgcggcgat ttcagcgaac agatttgtga aaacggcagc cagatatatg ctccgcccgc
    11701 gatcagttcg agtaccggac cggtacggga gtcgctgtcg ctaatccagt caaatgcgcc
    11761 gccattccac tgcccggaag tgtctgttat ggcctcaagc gcgcggctgc ggtgagcatc
    11821 agcttcctgc gcttcaccac gggcgttaca ggcgagggcg gtcagcagat cctccatcca
    11881 ggcgggcggc gggagaataa caccgggccg ctgttcgccc tgaaagcagg cgtggcggta
    11941 tatctcacag cggatcagct cgccaaatac ctgcgcttcg cgagtataat ttgcgtccat
    12001 ccgggcgcat agctggatct gttgcagtgc ccgcgaccag tcgcctgcca cgcacaataa
    12061 ctgaaacagg ctgtggcggc gggaggcctg cgctgggtgc atcctgacct gttgctctgc
    12121 catctgaatg ccctccacca cggaataatc ccgcagcagc gccgacaagg taatcggtag
    12181 ctcgttattg tttgtcatga tatatctctc catgttgtgc cgggtttatc cggtaatggc
    12241 tgtcaatgcc aatgatgcga tgctctttcc gtgtcagatc tggcagtacg gtgttatgcg
    12301 ctgtttccgg ggcgtattcc ggggaaagta aatgcaggat atcaggggga gtcttttcga
    12361 caggccagat agtttcatct tcgccagtgg catccagtac attgagaata gcgtcaatgc
    12421 ttaacccacc agtgaccata tcctgcaatg tctcggcttc ggtgaccagg tcacgcaatg
    12481 aatccagatc ctgcgaggag ggctcttcgt taaaaggaaa aggatcagca tggcgattaa
    12541 gatcttgttc gggagataac aacgcctcgc ggtattcctg ataaagtcgg gtcagggcat
    12601 ttcctttttc cgcaccttct ccggtggagg aggctccatg aacaggaatg atatcgaatg
    12661 gattttgtga tgtcagatgc tgctgcctga accaatccag atccagatat tcggttattg
    12721 cttccgacgt gatggcagtc tgcgatatcg tttttactgt gctcaggttc ggagatgatg
    12781 gattgccatg tacaaggcac tatgaagata acccccattc tatcaggtcg ccttcattca
    12841 gacgcatacg gtgatgaggt tccacaatct ggtcgtttac cgtacagcaa tattcggtag
    12901 actgattgat aatccaccat gcttcttcgt ggcgggtcag agtgagggca atctttacat
    12961 catcattcac cttaaaaaca ggaatccagg gagaggtaac ggtgaagata atttgtgttc
    13021 cttcagtcgc gtaaattgtg ttatcggcga tcccgtgcgc ctgtattttt tggagtcccc
    13081 acgtaatatt tggtttcatt gtgt
//
LOCUS       DAETQL010000097        13017 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.097, whole genome shotgun sequence.
ACCESSION   DAETQL010000097 DAETQL010000000
VERSION     DAETQL010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13017
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.097"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..319)
                     /locus_tag="K0W65_003284"
     CDS             complement(<1..319)
                     /locus_tag="K0W65_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HBI3096424.1"
                     /translation="MAISDTKLRTIYGKPYSGPQELADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFD"
     gene            complement(525..1244)
                     /locus_tag="K0W65_003285"
                     /pseudo
     CDS             complement(525..1244)
                     /locus_tag="K0W65_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="K0W65_003286"
     CDS             complement(1260..1592)
                     /gene="cusF"
                     /locus_tag="K0W65_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HBI3096425.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="K0W65_003287"
     CDS             complement(1616..2998)
                     /gene="cusC"
                     /locus_tag="K0W65_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HBI3096426.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            3155..3838
                     /gene="cusR"
                     /locus_tag="K0W65_003288"
     CDS             3155..3838
                     /gene="cusR"
                     /locus_tag="K0W65_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBI3096427.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3828..5270
                     /gene="cusS"
                     /locus_tag="K0W65_003289"
     CDS             3828..5270
                     /gene="cusS"
                     /locus_tag="K0W65_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBI3096428.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5420..7657
                     /gene="nrfB"
                     /locus_tag="K0W65_003290"
     CDS             5420..7657
                     /gene="nrfB"
                     /locus_tag="K0W65_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HBI3096429.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7644..10612
                     /gene="nfrA"
                     /locus_tag="K0W65_003291"
                     /pseudo
     CDS             7644..10612
                     /gene="nfrA"
                     /locus_tag="K0W65_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10613..11503
                     /locus_tag="K0W65_003292"
     CDS             10613..11503
                     /locus_tag="K0W65_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBI3096430.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11686..12447
                     /gene="envY"
                     /locus_tag="K0W65_003293"
     CDS             11686..12447
                     /gene="envY"
                     /locus_tag="K0W65_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HBI3096431.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12493..12569)
                     /locus_tag="K0W65_003294"
     tRNA            complement(12493..12569)
                     /locus_tag="K0W65_003294"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12533..12535),aa:Arg,seq:tct)
     gene            12812..>13017
                     /locus_tag="K0W65_003295"
     CDS             12812..>13017
                     /locus_tag="K0W65_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBI3096432.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3187 a         3375 c         3305 g         3150 t
ORIGIN      
        1 tgtcgaacca gtagtcgaga cactgggcga ccgtcatgga gttctccacc ttctcttcaa
       61 aataagtgcg tggatccgtt cctgagaaat agagctttcg caagtcagca gtgatctgtc
      121 tggcatcctt caaagacagg gatgggtatc gaccaagccc aagtcgatta ggcttgccat
      181 gccagcgata gcggtactgg aactggatga cccccttcgg tgaaattcgt acgctgaggc
      241 catcagcatc agccaattct tgtgggccag aatatggttt accataaata gtacgcagtt
      301 ttgtgtcgct tatagccata aataatattt ggtacgctca gaaataatgg tttggtacac
      361 atctggtaca caatatcaca tggacaacag cgcacagtaa acaactatat ttgacgttcg
      421 tagacatact caggtgaaaa aaaggttgtt tttcgagtta taacagacag ttaattaact
      481 acagtggaca atgctagaca atgacagaca cagataagca acctaccttc ctctttcgcg
      541 accacgttat ctttggcgat attcattccc gcgcgcagat caaacgcggt gatcacgcca
      601 tcaatgggcg ctttgagcgt aaagcgagtc tggatttttt gcgtggcgat caggtggcga
      661 atatccgcct ccggcattcc cgccagtcgc agccgctcaa gaatgccttc agtctgggtc
      721 gccgtaccgc cggtttcgcg caacagtaaa tactcactct gcgcttccac ccagtcggga
      781 atggtcaggt cgagaagcgg tgtgcccttt tgtactttat cgcccacggt aagcggatac
      841 accttgtcga taaacccggc agcgcgagcc tgcacaatgg catactgata ctcgttgtaa
      901 ctgacattcg tcgggaaact ctgggcaaaa gtcagcggcc cgcgcgtgac ggtagccgtt
      961 ttcaccccca gattctgcgt ctgagtcggg tcaatgcgca caccagacgc agaactctct
     1021 tcatcggcat atttcggcac cagatccata tccataaacg gcgatttacc tggtttatcg
     1081 aaccgcgtat tgggatacat cgggtcgtac cagaataaga ttttacgttc tgcggtcggc
     1141 attttttctg cgtgcggttc cgcctggtca aaccaggtaa aacctgccgc tgaaataata
     1201 ccgcccgcga tcatgctgcc gataataagc gcgatttttt tcatctcatt aaacctgggt
     1261 tactggctga ctttaatatc ctgtaataaa gaaaggttgc cctgctggac aaaattaaac
     1321 gccactttgt cgccggtttt aatttcactc attttcgtct gcggggtgat ggtaaagcgc
     1381 atggtcatct ccggccagtt gacggcagca atcggatcgt gatggatggt gatttttttg
     1441 ctttccagat caacaccttt taccacaccg gtggcactaa tgacctgtgg ttgtgcttcg
     1501 ctcatggttt catgatgatg ttcgttagcc tgggcattaa agccaataac ggtaaacaga
     1561 ctgaacattg cgacttgcag tgcttttttc atttattctc tcctggagtt aaaaattatt
     1621 gctgccaacc gccccctaag gcggtataca aataaatttc gttaacctga cgggcataat
     1681 tcaggtcaag taaagtttgt cgggttgcaa ataaagaacg ctcggcatcc agcacttcca
     1741 gataacttac cgcgccgtgc tgatataatg cccgcgcccg ttgcagggta atttgcagcg
     1801 acgccagata acgctgctgg gcgctgattt gatcgtccag gctttgacgt aatgccagcg
     1861 catctgccac ctctttaaag gcgttctgga ttttctgttc ataattcacc accgactgct
     1921 gctggcgaat ttcggcgata tccagattgg cctggttgcg tccggcatta aaaatgggga
     1981 tctcaatttt aggaataaaa ttccacatcc cgctgctagc gttaaataat gacgacagat
     2041 cgctgctggc ggttgatatt ccgctggtca ggctgataga cgggaaaaat gccgcacgcg
     2101 cagcaccaat attagcatta gccgccatta acgcgtgttc agcttccata atatccgggc
     2161 gctgcaataa gatttgcgac gacaagcccg ccggtaattt aacgctttgc aggctgccgc
     2221 tgtttactgt ctgcgcttgc ggtagcttgc cgtagcttcc caataacagt tgcaatgcat
     2281 tattcgcctg cgccagttcc ccctgacgtt tagcgatgtc actgcgggta ctttctatca
     2341 ccccgcgagc ctgttccagc gccagaacat tgctgctacc ggtcaacagt tgtttttcga
     2401 caaacgcata tgactgctga taattacgca gcgtttcttc ggctatttgc aattgcgcat
     2461 acgccagctg ctgattgaaa tagctttgcg cgacattaga aaccagcaaa atatgcaccg
     2521 cgcgctgggc ttcctcagtg gctaaataat tttggcgctc ggcttcgctc atgttcttta
     2581 agcgaccgaa aaaatcgaga tcaaagctgg cgttaaggcc agtcgagaac tcccgcgtcg
     2641 tggctgaatc gcctttaaga ttgccgctcc agctgccgct gccctcgcca ttgagctgtg
     2701 ggtagcggtc ggcatcggtc agacgatatt gcgcccgtgc ctcctgcact ttcagcgtcg
     2761 ccatgcgcaa atcccggtta ttcaccagcg cctcgctaat cagcgtcttc acctgattat
     2821 caacaaaaaa ggtgcgccag cccacgttct ggtagttatc cgctgcgtta accaggccgt
     2881 tctggctgag tgagaactgc tgcggtacgg gcattaccgg acgctgataa tccggtgcca
     2941 gtgaacaacc agttagcgca agggccacac aaaatggcag aagtttacaa ggagacatag
     3001 gctcataatt tctggtgatt ttatgtcgcc aactttactc gccaggctct gattttccgg
     3061 tgacaggaaa atgacaaaat tgtcattttg ccaataagcg attgccatct gatcccgcta
     3121 ctctagaatt gcccgggcaa catgcggagg aaatatgaaa ctgttgattg tcgaagatga
     3181 aaagaaaacc ggagaatact tgaccaaagg gttaaccgaa gccggttttg tggtcgattt
     3241 ggccgacaac gggctgaatg gctaccatct ggcgatgacc ggtgattatg acctgataat
     3301 cctcgatatt atgctgccag acgtgaacgg ctgggatatc gtgcgcatgt tgcgctccgc
     3361 caataaaggg atgccgattc tgttgcttac cgcgcttggc accattgaac atcgcgtcaa
     3421 ggggctggag ttgggggcag atgactacct ggtgaaaccg ttcgcttttg ctgaactgct
     3481 ggcgcgggtg cgcactctcc tgcggcgcgg ggcggcggtg attatcgaaa gtcagtttca
     3541 ggttgccgat ctgatggtcg atctcgtcag ccgcaaagtc acccgcagcg gcacgcgcat
     3601 cactctgacc agtaaagaat ttactctgct ggagtttttt ctccgccatc agggcgaagt
     3661 gctgccccgc tcgcttatcg cctcgcaggt atgggacatg aatttcgaca gcgacactaa
     3721 cgccattgat gtggcggtga agctgctacg cggcaaaatc gacaacgact ttgagccgaa
     3781 gctgattcaa accgtgcgcg gcgtgggtta catgcttgag gtgccggatg gtcagtaagc
     3841 catttcagcg cccgttttcg ctggcaaccc gcctgacctt ttttatcagc caggccacca
     3901 tcgcggcgtt tttcgccttt gcatggatca taatccactc agtaaaagtg cattttgccg
     3961 agcaggacat taatgattta aaagagatta gcgccaccct tgaacgggta ctaaatcacc
     4021 ctgacgaaac gcaagcccga cgcttaatga cgctggaaga tatcgtcagt ggttattcca
     4081 acgtgttgat ttccctggca gatagccacg gcaaaacggt gtatcactcc cccggtgcgc
     4141 ctgatatccg cgagtttgca cgtgatgcca tacccgataa agacgcgcgg ggtggcgagg
     4201 tgtatctcct ttccggcccg acgattatga tgccaggcca cggtcacggg catatggaac
     4261 acagcaactg gcggatgatt aacttgccgg ttggcccgtt ggtggacggc aaaccgattt
     4321 atacgctcta catcgcgctg tcgatcgatt ttcatcttca ttacataaat gatttgatga
     4381 ataaacttat tatgaccgca tcggtaatca gcatcctgat cgtctttatc gtgctgttgg
     4441 cggtacataa aggtcacgcg ccgatccgca gcgtcagccg tcaaatccag aatatcacct
     4501 cgaaagatct cgacgttcgc cttgacccgc agaccgtgcc gattgagctg gaacagctgg
     4561 tactgtcgtt caaccatatg atcgagcgta ttgaggatgt ctttactcgc cagtccaatt
     4621 tctcagcgga tatcgcccac gaaattcgca caccaattac taatctcatt acgcaaacgg
     4681 aaatcgccct cagccagtcg cgcagccaga aggagctgga agatgtgctc tactctaatc
     4741 tcgaagagct gacgcgaatg gcgaaaatgg tcagcgatat gctgtttctc gctcaggccg
     4801 ataacaacca gctaatcccc gaaaagaaaa tgctcaacct ggcggatgaa gtcggcaaag
     4861 tgttcgattt tttcgaggcg ctagcggaag atcgcggcgt ggagttgcgg tttgttggcg
     4921 acaaacgtca ggttgcgggc gatccgttga tgctgcgtcg ggcgctaagc aacctgcttt
     4981 ctaatgccct tcgttatacg ccaaccagag agacaattgt agtacgctgt cagacggtcg
     5041 atcaccaggt gcaagtttcc gtcgaaaacc ccggtacgcc cattgcgccc gagcacttac
     5101 cgcgattgtt tgaccgtttc tatcgcgttg acccctcccg ccagcgaaaa ggtgaaggta
     5161 gcggtattgg gctggcgata gtgaaatcga ttgttgtcgc gcataaaggc acggttgcgg
     5221 taacgtcaga tgcgcggggg acaaggtttg ttatcacatt acccgcttaa ttattcagca
     5281 aacggcaact tttataacca gtgtaaaaat aacgtgccgc aatagtcata catattaatg
     5341 gtaaaaagct gtcacaattc ataaaaaacc ttaatatacg ccaccctaaa cataaccagc
     5401 gttaatgtaa ggtttttgtg tggactggct tcttgatgtt tttgctacct ggctctacgg
     5461 cttaaaagta atcgcgataa cgttagcggt catcatgttc atcagcgggc tggacgattt
     5521 ttttattgat gtcgtctact gggtacgccg cattaaacgc aagttgagtg tttatcgccg
     5581 ctacccgcga atgagttacc gcgaactgta taaaccagat gaaaaaccgt tagcgattat
     5641 ggttccggcg tggaatgaaa cgggcgtcat cggcaatatg gccgagctgg cggcgaccac
     5701 gctcgactac gaaaactatc atatctttgt tggcacctac cccaacgacc ccgatactca
     5761 acgtgatgtt gacgaagtgt gcgctcgctt tccgaacgtg cataaggtag tctgcgcgcg
     5821 tcctggtccc accagcaaag ccgactgcct gaacaacgtg ctggacgcca tcacccagtt
     5881 tgaacgtagc gccaatttcg cttttgctgg ttttattctg catgacgccg aagatgtgat
     5941 ttcaccgatg gaattgcgtc tgttcaacta tctggtcgag cgtaaagatc tgattcagat
     6001 cccagtgtat ccgtttgaac gcgaatggac gcacttcacc agcatgactt acattgatga
     6061 gttttcagaa ctgcatggca aagatgttcc ggtgcgtgaa gccctcgccg gacaagtgcc
     6121 cagcgcaggc gtcggcacct gtttcagccg ccgcgccgtg accgcactgt tagctgacgg
     6181 taacggtatt gctttcgacg tgcagagtct tactgaagat tacgacattg gcttccgcct
     6241 gaaagaaaaa ggtatgacgg aaatttttgt ccgttttccg gtggtggacg aagccaaaga
     6301 acgcgagcag cgtaaatttt tacagcacgc acggacgtca aacatgatct gcgtgcgcga
     6361 atatttcccc gatacctttt cgactgcggt tcgacaaaaa tctcgctgga tcatcggcat
     6421 tgttttccag ggctttaaaa cccataaatg gacctccagc ctgacgctga actactttct
     6481 ctggcgcgac cgcaaagggg caatcagtaa ctttgtcagc ttcctcgcga tgctggtgat
     6541 gatccagctt ttgctgttgc tggcgtatga aagtttgtgg cccgatgcct ggcatttcct
     6601 ttctattttt agcggcagcg catggttaat gaccctgctg tggctaaact ttggcttgat
     6661 ggttaaccgc atcgtgcagc gagtgatttt cgtcactggc tactacggcc tcacgcaggg
     6721 cctgctttcc gtcctgcgtc ttttctgggg caacctgatt aactttatgg ctaactggcg
     6781 cgtgctgaaa caggttcttc agcacggcga tccgcgtcgc gtcgcgtggg ataaaacaac
     6841 gcatgacttc cccagcgtca ctggcgatac ccgctcgttg cgcccgttag gtcaaattct
     6901 gctggaaaat caggtcatca ctgaagaaca actcgataca gcactgcgta atcgcgtcga
     6961 aggtctacgc ctgggcggtt caatgctgat gcaggggctg attagcgccg agcagctggc
     7021 acaggcgctg gcagagcaaa acggcgtggc gtgggaatcc atcgatgcct ggcagatccc
     7081 gtcctcgctg attgccgaaa tgccagcctc cgtagcgctg cattatgcag tactgccgct
     7141 gcgtctggaa aatgatgagt taattgtcgg cagtgaagat ggtattgacc cggtttcgct
     7201 ggcggccctg acgcgtaaag tcggacgcaa agtgcgttac gtcattgttc tgcggggaca
     7261 aattgtcaca gggttacgcc actggtatgc acgccgacgc ggtcacgatc cgcgggcaat
     7321 gttgtacaat gcggttcagc atcagtggct cacggaacag cagaccggtg aaatctggcg
     7381 gcaatatgtg ccgcatcagt tcctgttcgc cgaaatactg accacgctcg gtcatattaa
     7441 tcgttcagca attaacgtgt tgttattgcg ccatgaacgc agttctctgc cgctcggcaa
     7501 gtttttggtc accgaaggcg ttatcagcca ggaaacgttg gatcgcgtcc tgacaattca
     7561 acgcgaatta caagtttcga tgcaatcact attactcaaa gcaggtttaa acacagaaca
     7621 ggttgcgcaa ctggagtccg aaaatgaagg agaataacct taatcgcgtc atcggatggt
     7681 ctggtttact gctgacgtct ttattgagta ccagcgcact cgcagacaat atcggcacca
     7741 gcgcagaaga gctggggctg agcgattatc gccattttgt tatttatccc cgtctcgata
     7801 aggcgctgaa ggcacagaaa aataacgacg aagcaaccgc catccgcgaa tttgaatata
     7861 tacaccaaca ggtgccggat aatattccgc tgactttata ccttgcggaa gcctatcgtc
     7921 attttggtca tgatgaccgg gcgcggctgt tgcttgagga tcaactgaaa cgtcacccag
     7981 gagatgcccg acttgagcgc agtctggcgg ctattccagt tgaagtgaaa agcgttacga
     8041 ctgttgaaga actgcttgcc cagcaaaaag cgggcgacgc tgcgccgacc ctgcgttgtc
     8101 gcagtgaagt cgggcagaat gccctgcggc tggcacaatt acctgtcgcc agagcgcaac
     8161 tgaacgatgc gacgtttgct gcatcgccgg aaggaaaaac gctgcgaacc gatctgctgc
     8221 aacgggcaat ctacctgaaa caatgatccc aggcagatac gctatacaat gaagcacgcc
     8281 agcagaacac attaagcgcg gcagaacgcc gtcagtggtt tgacgtgctt cttgccgggc
     8341 agctggacga tcggatcctg gcactgcaat cacaggggat cttcaccgat cctcagtcat
     8401 atattactta cgcgaccgcg ctggcttatc gtggcgaaaa agcacgcctc cagcattatc
     8461 tcattgaaaa taagccgctg tttaccacgg gcgcacaaga gaaaagttgg ctctatctgt
     8521 tatctaaata cagcgccaac cccgttcagg cattggcgaa ttatacggtg cagtttgccg
     8581 ataaccgcca gtatgttgtt ggcgcgacgc taccggtgct gttaaaagaa ggtcagtacg
     8641 acgcagcgca aaaactgctc gccaccctcc ccgccaatga aatgcttgag gagcgttatg
     8701 ctgtcagcgt ggcgacccgt aacaaggctg aatctctgcg tctggcacga ttgctgtatc
     8761 agcaagaacc gacaaatctt ccccgcctgg atcaactgac ctggcaactg atgcagaacg
     8821 agcagtcacg cgaagctgcc gatttattgc tgcaacgcta tcctttccag ggcgatgcgc
     8881 gtgtcagcca gactttaatg gcgcgactgg cgtctctgct ggaaagtcat ccttacctag
     8941 caacgccggc gaaggtggcg attttatcga aacccttacc gctggcggag caacgtcagt
     9001 ggcaaagtca gttgccgggt attgcagata attgcccggc aatagttcgc ttgctgggcg
     9061 atatgtcgcc ttcctacgat gctgccgctt ggaaccgtct ggcaaagtgt tatcgggaca
     9121 cgctacccgg tgtggcgttg tatgcatggc ttcaggccga acaacgacaa ccgagcgcct
     9181 ggcaacatcg tgcggtagcc tatcaggcgt atcaggttga ggactacgcc accgcactgg
     9241 cggcctggca gaaaatcagt cttcacgaca tgagcaatga tgatctgctc gccgccgcca
     9301 acaccgctca ggcagcggga aatggcgcag ctcgcgatcg ctggcttcaa caggcagaac
     9361 aacgtgagct gggaaacaat gctcaatact ggtggctgca tgcgcgacgt tatatcccag
     9421 ctcagccaga actcgcactg aacgatctca cgcgctcaat caatattgcc ccttctgcca
     9481 actcttacgt tgcgcgggcg acaatttatc gccaacgtca taatgttccg gccgcggtga
     9541 gtgatttgcg cgccgcgctg gaactggaac caaataatag caacacccag gcagcgcttg
     9601 gttacgcctt gtgggatagc ggtgatatcg cacagtcgcg ggaaatgctc gaacaggcgc
     9661 ataaagggct tccggacgat ccggcactga tccgacaact ggcctacgtg aaccagcgtc
     9721 tggatgacat gcctgcgacg cagcactacg cccggctcgt gattgatgac attgataatc
     9781 aggcataacc ccactgaccc cagagcaaaa tcagcaacgc ttcaatttcc gccgtttgca
     9841 tgaggaggtc ggtcgccgct ggacgttcag tttcgattct tccatcggct tgcgttccgg
     9901 cgcaatgagt accgctaaca ataatgtcgg cggtgcagcg cctgggaaaa gctatcgtag
     9961 ctacggacaa ctggaagccg agtaccgcat cggacgcaat atgctgctgg aaggcgacct
    10021 gctctcagtt tatagccgcg tctttgccga taccggagaa aacggggtga tgatgccggt
    10081 gaaaaatccg atgtccggca ccggtctgcg ctggaagccg ctgcgcgatc agatcttttt
    10141 cctcgccgtc gaacagcagt tgccgctgaa cgggcaaaat ggcgcatccg ataccatgct
    10201 gcgcgccagc gcctcattct ttaatggcgg caaatacagc gacgaatggc acccgaacgg
    10261 ttcaggctgg tttgcccaaa acctgtacct cgatgcggcg caatatatcc gccaggatat
    10321 tcaggcgtgg acggcagatt atcgcgtcag ctggcatcag aaggtagcta acggacagac
    10381 tattgagcct tacgctcacg ttcaggacaa cggctatcgt gataaaggca ctcagggcgc
    10441 gcagcttggc ggtgtcgggg tccgctggaa tatttggacc ggcgagacgc actacgacgc
    10501 ctggccgcac aaagtcagtc tcggcgtcga gtatcaacat acctttaagg cgattaatca
    10561 acgtaacgga gagcgcaaca acgcgtttct caccattgga gtgcactggt aaatgcgtaa
    10621 gttcattttc gtattgctga cactgctttt ggtcagccct ttttcctttg cgatgaaagg
    10681 tattatctgg caaccacaaa accgagatag tcaggttacc gatacccagt ggcaggggct
    10741 gatgagtcag ttacgttttc aaggcttcga taccctggtt ttgcaatgga cccgttacgg
    10801 cgatgcattt acccagccag aacagcgcac gttattgttt aagcgtgccg cagctgcgca
    10861 acagactggc ctgaagctga ttgtcgggct gaacgccgat ccggaatttt ttatgcacca
    10921 gaaacagtcg tctgcagcgc tggaaagcta tcttaatcgc ctgctggctg ccgatctcca
    10981 gcaagccaga ttatggagcg ccgcgcctgg cataacgccg gatggctggt acatcagcgc
    11041 ggaaattgac gacctgaact ggcgcagcga agccgcccgt caacctttgc taacatggtt
    11101 aaacaacgcg cagcggctga ttagcgatgt ttcagcaaaa ccggtttata tcagtagttt
    11161 tttcgccgga aacatgtcgc ccgatggcta tcgccaactg ctggaacaag ttaaagcaac
    11221 cggagttaat gtctgggtac aggatggcag cggcgtggat aaacttaccg ctgaacagcg
    11281 tgaacgttat ctacaggcca gcgccgattg ccaaagctcc gccccagcaa gcggcattgt
    11341 ttatgaactg tttgtcgccg gcaaaggcaa aacctttaca gcgaaaccga aaccggacgc
    11401 agaaattgcc tcgctgttag cgaaacgttc ctcttgcggt aaagacatgc tctatttctc
    11461 tctgcgctat ttgcccgtcg cgcacggcat tctcgagtat taaatcccct ccaggtaagt
    11521 cgggtacgac ctggcttacc tctttcgctc tcgtcagaat tccatcaaga ttaattgcta
    11581 aaaagcatgc taaagaaaga agatcgttat cggcgaacgg gccgatcact catgcaggaa
    11641 gcatagatcc tttgagcgga gtttctgata tctgaggagt gcgaaatgca attgagcagc
    11701 agtgaacctt gcgtggtgat cctgaccgaa aaagaggtag aggtaagcgt caataaccat
    11761 gctacgttta cccttccgaa aaactacctg gccgccttcg cgtgcaacaa taacgtcatt
    11821 gaactctcaa cgttaaatca cgtattaatc acccacatca accgtaacat catcaacgat
    11881 tatctgttgt ttttaaataa gaaattaacc tgtgtaaagc cctggtcgcg gctagcaacc
    11941 ccggttatcg cttgtcatag ccgtacaccg gaagtgttcc ggctagccac caaccacagc
    12001 aagcagcaat ccagcaaacc ctgcgaagcg gagttgacgc gcgcattact ttttaccgta
    12061 ttgtctaact ttcttgagca atcgcggttt attgccctac tgatgtatat cttacgcagc
    12121 agcgtccgcg acagcgtctg ccgcattatt caaagcgata ttcagcatta ctggaatctg
    12181 cgaattgtcg ccagttcgct atgtttaagc cccagcctgc tcaaaaagaa attaaaaaac
    12241 gaaaatacca gctatagcca gattgtcaca gagtgtcgta tgcgttacgc cgtacagatg
    12301 ttattgatgg ataacaaaaa tatcactcag gtggcgcaat tatgtggcta tagcagcacg
    12361 tcgtacttta tctctgtttt taaggcgttt tacagcctga caccgttgaa ttatctcgcc
    12421 aaacagcgac aaaaagtgat gtggtgaagg gcaaagcgga aacggataag acgggcataa
    12481 atgaagaaga aatggcgcgc cctgcaggat tcgaacctgc ggcccacgac ttagaaggtc
    12541 gttgctctat ccaactgagc taagggcgcg ttgataccgc aatgcggtgt aatcgcgtga
    12601 attatacggt caacccttgc tgagtcaatg gcttttgatc tggttgctga acaagtgaac
    12661 gaccgcgtct gattttctga tttatttcgc tatagcggca aacaaacgca caccgctgcg
    12721 cgtctgaatc aagaaaaccc gtattttcat gtatcaaagt acaatttacc gccctaacgg
    12781 aaaattgtcc gctcctatga gactggtaac tatgaaacca acgtcggtga tcattatgga
    12841 tactcatcct atcatcagaa tgtctattga agttctgttg caaaaaaaca gtgaattgca
    12901 gattgtcctg aaaacggatg attaccgcat aaccatcgat tatctccgaa cccgtcctgt
    12961 tgatttaatc attatggata tagacttgcc cggaacagac ggttttacct tcctgaa
//
LOCUS       DAETQL010000098        12907 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.098, whole genome shotgun sequence.
ACCESSION   DAETQL010000098 DAETQL010000000
VERSION     DAETQL010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12907
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.098"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="K0W65_003296"
     CDS             complement(128..1579)
                     /gene="uxaB"
                     /locus_tag="K0W65_003296"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBI3096433.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(1806..3224)
                     /locus_tag="K0W65_003297"
     CDS             complement(1806..3224)
                     /locus_tag="K0W65_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBI3096434.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEVLKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3378..3722)
                     /locus_tag="K0W65_003298"
     CDS             complement(3378..3722)
                     /locus_tag="K0W65_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HBI3096435.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="K0W65_003299"
     CDS             complement(3722..4648)
                     /gene="glsB"
                     /locus_tag="K0W65_003299"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBI3096436.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4712..5647)
                     /locus_tag="K0W65_003300"
     CDS             complement(4712..5647)
                     /locus_tag="K0W65_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBI3096437.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5727..6842
                     /gene="yneK"
                     /locus_tag="K0W65_003301"
     CDS             5727..6842
                     /gene="yneK"
                     /locus_tag="K0W65_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HBI3096438.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDTLSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6992..8182
                     /gene="ydeA"
                     /locus_tag="K0W65_003302"
     CDS             6992..8182
                     /gene="ydeA"
                     /locus_tag="K0W65_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBI3096439.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8207..8872)
                     /gene="marC"
                     /locus_tag="K0W65_003303"
     CDS             complement(8207..8872)
                     /gene="marC"
                     /locus_tag="K0W65_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBI3096440.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            9084..9518
                     /gene="marR"
                     /locus_tag="K0W65_003304"
     CDS             9084..9518
                     /gene="marR"
                     /locus_tag="K0W65_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBI3096441.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9538..9921
                     /gene="marA"
                     /locus_tag="K0W65_003305"
     CDS             9538..9921
                     /gene="marA"
                     /locus_tag="K0W65_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBI3096442.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9953..10171
                     /gene="marB"
                     /locus_tag="K0W65_003306"
     CDS             9953..10171
                     /gene="marB"
                     /locus_tag="K0W65_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBI3096443.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="K0W65_003307"
     CDS             complement(10202..11101)
                     /gene="eamA"
                     /locus_tag="K0W65_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HBI3096444.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11365..12483
                     /gene="ydeE"
                     /locus_tag="K0W65_003308"
     CDS             11365..12483
                     /gene="ydeE"
                     /locus_tag="K0W65_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HBI3096445.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12610..12705
                     /gene="mgtS"
                     /locus_tag="K0W65_003309"
     CDS             12610..12705
                     /gene="mgtS"
                     /locus_tag="K0W65_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HBI3096446.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3402 a         3299 c         2948 g         3258 t
ORIGIN      
        1 gtcaagctta caacattaac aggcgctgca tgtgacacct gttaatgata aaggaatata
       61 ggatgttgta actaacatag tgaaataaga aacccggtcg aaaccgggtt cagaagtaac
      121 ggtgttatta gcacaacgga cgtacagctt cgcgcatccc tttttcaaga atcgcatcca
      181 ggtcattagc aacctgctcg accagaccag gcacttgcgt caggtcctgc tcccagtggt
      241 ctttttctgc caacacaatc gctaccagtt cctgggtacc gataacacgg tcacgatgct
      301 ggctccagag ttgctgataa cgttccagcc agtgtgcatc gtcctgtacc ggatatgttt
      361 ctccgttacg ctcaccacga tagaacgcaa tcaatgccgc taatgcgaaa gtaaggcgcg
      421 ccggaagtgt gccatttgcc ttctgccctg ccagcagctg cggcaggatg cgggtgcgga
      481 acttggtcat accgttgagc gcgatagaca gcagctgatg cttaatgtac gggttacgga
      541 aacgcccggt gactgcactg gcgaaagatt ccagttcatc acgaggcaaa tccagtaccg
      601 gaataatttc ttcgtagatg gctttttcaa cgaatgcgca aatttcagca tcgttcatcg
      661 cttcacctac ggtatccagc cctgcctgga acgccaccgg caccagcgcg gtgtgcgcac
      721 cgttgaggat tgccactttg cgttctttat acggtttaat atcgtcaaca atcagcacgt
      781 tgagcggata tttgtccagg cgcagctcag tcgctaagga tttcggcccc tgaatcacaa
      841 acaggtaaaa gtgttcagcg gtgtcgagga aaccatcgtg ataacccagt tcttcttcca
      901 gtttagccac ttcatcgcgc ggataaccgg taacgatacg gtccaccagc gtagaacaga
      961 agctgttagc ctgatccagc cattgaataa atgcttctgg cagtgcccac tcttgcgcat
     1021 agcgcagcac cagttcacgc aacgcgtcgc cattgtagtc aatcaactca cacggaatga
     1081 tgatccaacc tttatccagc gcaccgttga aatggctaaa gcgttcgaac agcagacggg
     1141 tcagttttgc cggatagctt accgctggcg cgtcatcgaa tttatcgccc gcatggtagc
     1201 tgatacccgc ttcggtggtg ttagagaaaa caaagcgcat ttccgggttg tgcgccagtt
     1261 tcaggaattc atcgtattca ctgtagacgc tgatttcacg attaaccgag cgaatcagac
     1321 gcgcgtcgct gaccgcttcc cccttctcat tcaggccacg gataatggtg gtgtacagac
     1381 catcctgcgt gctcagtgac ggcgggaatg aagtttcaat cggacgaaca acgaccacgc
     1441 cagaattcag atcggtgtgc tcattcagga gatcgatttg ccagtcaaca aaggcgcgca
     1501 ggaagttacc ttcaccaaac tgaatgatac gttctggata ctgtgcaccg ggaaaatcgc
     1561 gacgatttag tgttttcaca atgggttccc ttctgattag tcatacaacc cgtttgaatt
     1621 ggtgcgacag gttagcaaac tttaatacgc cgaacccctg ttttgatcaa ctcctgatga
     1681 ttaatgagca gttttatgag aaaagtgtgg cgcggatcat ggtttaatcg aggaaatacg
     1741 ccttttcctg gatcataaag tggtagaaca cattgcattc aaatcgcgcg gaatgaataa
     1801 agatgtcaga caacttcctc accgtaacgc atagtgctgg tacggttgcg cccatctttc
     1861 ttcgagcgat acagacacgt atcggcttcg accatcaatt tattaaaatc atcggtcagc
     1921 gttcggtacg atgcgcgacc actaccgacg ccaatactta ccgtgagata aagcgttttt
     1981 tgttgccagg tgaatggttg cagttcaacg cctttacgga ttttttccgc cattagcaga
     2041 ccatcgacag gattcaccga cggcactgca acagcaaatt cttcgccgcc catgcgcgcc
     2101 accagcccct tatcaccgac aatcttctga atatgccggg caaacacgct taacacttta
     2161 tcgccacatt catgtccata gttatcgtta atgcttttga aatagtcgat atcaagcagc
     2221 atgaccgtca gatgttgtgt ctgtttcagc gatggacttt tcagcacctc ataaagaccg
     2281 gaccgtgagt aaacctgagt cagaaaatca aagtcggctc gcagcgcaac ttgcttcatt
     2341 agcaaattga tcgctgccac gctaaaagaa accataattg ggcatatcgc catcgtggca
     2401 ataccgagac gtgcggagaa catttccgga atggagaacg gcgaaccgac cgagatatca
     2461 atcaccgaat ttgccaccag tacgatttcc accgcaccgg tgacaaaggt caacagacat
     2521 gttacctgcg gcgtatagcg cactgcacac cagattaatg caggcagcgg gaacgccaga
     2581 ctccccgctc cgccaatgac taccgaggca ataaccgaca caataaacgc gatggcgggc
     2641 atcatctgtt ctgctttaaa gcgcggcagc actccaggaa tagccaacgt cagcatacaa
     2701 ggcacgatca acacgcccgt tgagaattgc tcactgaacc agtccgcaag caaaggccag
     2761 aaatccagac tatcaatact gaccgaacca atcgccccga caatagcgca taataatgcg
     2821 gcaatcagac agtaattaaa tagccgtaaa gcacttactg gctcatactt atttttcccc
     2881 agacgcttat cccgcacgac cagtaaggcg acggtaacaa taaacatcat attggagaaa
     2941 ttgataacca gtgaaaccag tccccattcg gtggttatgg catcataaac cagcatcgcc
     3001 acataactga tcgcatagta atgcagtcga ttaagccaga catagcgggc aaacaccccc
     3061 gccattacgc cattcagcgg ccagaaaaga gaaagttcgt gaaccagccg taactctgca
     3121 ccaataaaat agaacagcgt ggttagcgca aaaatagcga tgctattacg tagcagatga
     3181 ccttcctgga acaaacggaa agttgaaatg gggtgtacat gcattaatta tctgtccata
     3241 caattgcggg tggcaattat ctcaacgtga taagtaaatt ctcataaagg atagcatgtt
     3301 cgctaacagc ggcggcaaat aaaggtaccc tgggaatgct attttatcgt tttccgcaat
     3361 tctcatggct gattcatcta tacaaccagc cagtgataga ttaccgccac gatataacgt
     3421 tgttgttctt cgcttaacga aacaccctgc ggaatgttat gccacttagc cagacaaccg
     3481 cgacagcagg tcgcggtggc gtgctgagcg ataaacaccg gatgcccgcg catgggggtt
     3541 tgcttaccgt cattagccgg taatgccgga gcaagacgtt tagcgacaaa atccgccgca
     3601 tgttgttcga tgactggcgc gcctttctcc aggcaatact gacgctcttt catgcccaga
     3661 cgaaagcgag agcgaaattt tgaacgggat aaacgcgcga agagtggatc gagagactgc
     3721 attaataaac cgaacgccct aactgtttcg tcaattgttc aaggacggcg atacccgcaa
     3781 gcgagttacc tgcatcatcc agttccggac tccagactgc gatggccatt tcatgcggaa
     3841 caatcgccac aataccgcca ccaacgccag atttcgccgg tagtcccacc cgccaggcaa
     3901 actcccccgc gttctggtac ataccactgg tcgccatcag cgcgttaatt tgccgcgcct
     3961 gcattggcgt caccacaggt tcatcaatat gaatagcttt cccctgatta gccagaaaga
     4021 caaacgtccg ggccagctct acacagctca ttttcagagc gcagtaatga aagtagtttt
     4081 gcagaacggt tgtcacgtca tgatggaaat tgccaaacga cttcatcagc caggcgatag
     4141 ccgcatttcg cgcggaatgt tcaaattcgg aacgcgctac caccgtatcg tatgaaatat
     4201 cagacacacc gcttaagccg cgcacgactt ccagcatacg ttgccgtggt gcgcttagtc
     4261 gcccttgcaa catatcgcag accaccagcg caccggtatt aatgaacgga ttacgcggaa
     4321 taccctgttc catttccagt tgcactaaag aattgaacgg tgatccagac ggatctttgc
     4381 cgacgcgttg ccagatttcc tcttcggagt aatgacgcat ggcgacaacg agactcagca
     4441 ctttggaaat agactgaatg gaaaaacgtt cttgcgcgtc tccggcctga aaaagctgtc
     4501 cgtcaacggt acagatagca atccccaatc gggaaccgtc tactgtagcc agcgccggaa
     4561 tataatccgc gactttaccc tgaccaatga gcggccgcac ttgccgcaag atgttttcta
     4621 aaattgcatt atccatggcg actgccactt tctactcctg gaccgcaggt ctgaaaagac
     4681 ctgcgagtat atcagagctg aatatgtcgc gtcagatccg gtctttccac actgtctgga
     4741 tattacagaa ttcgtgtaag ccgaaatggg aaagctcacg accaaagcca ctctttttca
     4801 cgccaccaaa ggccactcgc gcgtcgctgg cacaataacc attgataaac accccaccgc
     4861 attccagacg tgccgccatc tgtctggcct gtgtttcgtc ggtggtaaaa atggtcgctg
     4921 aaaggccgaa ctcactatca ttagccagtt ccagcgcatg ttctgcatct ttcgcaatgg
     4981 tgattgcagc aacggggcca aacatttctt cccgaaacgc ggtcatttct ggggtaacat
     5041 tcgccagaac cgttggcgga tagtagttac ctgctccagc catcttttcc ccgcccagta
     5101 acaaacgcgc accctgcgcc agggttttct ccacctgatg atgcagctca tcacgtaaat
     5161 caaaacgagc cattggtccg agagcgttct cttcatcacg gggatcgccc attttcaagg
     5221 ctgccgcagc tgacacaaaa cgttcggtaa atgccgaagc aattccctct tcgataataa
     5281 agcgttttgc cgctgcacaa acctgtccgg tattctgata acgtccggct accgccgctt
     5341 taaccgccag ttccagatcg gcatcgttaa gcacaataaa tggatccgaa ccgcccagtt
     5401 ccagtacgca ttttttcagt gccgctccag cacgaacact tccggtcacc gtgacagcag
     5461 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     5521 atacgccttg cgggattccc gcatctttaa acacttgggc aatgagctga gcacagccca
     5581 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     5641 cacgcatcac ctgcctttga tagttcttgc agctcaaatg ccctatatag tgatgttccg
     5701 atgacttatg actatatggg gcaaatatgg ttacgccagt aagcatctgc aattacatat
     5761 ctcttcctga tgattttccc gctcgtaata ttgcacctca agtaaaagaa gttttaaagg
     5821 attttattga tacactcagt accataatat gtgatgaaga gtggcgcacg tctttaaaca
     5881 ccaactcagc cacaaaaaag atatttaaca accttgataa cttatcatat attcagagga
     5941 cttcttttcg gggtaacgac acactataca atgaaaaggt tcagtttaaa ctcacttatc
     6001 ctgtcaagaa tggaagacac aaagaaaata ttgaatttca ggtagtaata aatttaagcc
     6061 ctatttattt agataatttt cgccatgatg gagaaattaa tattttttgc gctcccaacc
     6121 caaagcctgt cactatgggg cgcgtatttc agaccggcgt cgagcgtgtg ctctttctgt
     6181 ttatgaatga ttttattgaa caatttccaa tgatcaacct tggcgccccc atcaaaagag
     6241 cgcatacacc acatattgaa cccctgcctc cggatcacca taccgctgca gattacttac
     6301 gccagtttga tttgcttgtc ctgaatttta tctctcgcgg taattttgtc atactccccc
     6361 gattatggaa taactctgag gttcacagat ggttcgtcaa taaagatcct aacttgatca
     6421 ccgccattct cgacataacg gacagcgaat taaaagagga tttattgcaa agcctgatgg
     6481 attcattagg ttctaacaaa catgtacaac ccgaagtctg catctgcttc ttatcccttt
     6541 tagcagaaca agaatctcct cattttcagg acttgttttt atttttcgcc aatatgttat
     6601 tgcactatca ccaatttatg aatcccaatg aaagtgatct gaatgacgtg ttgatgccag
     6661 catcattaag tgatgataaa attatcaaac atatggcgcg caggaccctc aaactgtttg
     6721 taaaaaatga aacgccgcca aaagttactc acgaagacct ggtgaaaaac aggcctcgct
     6781 cccctgtcag accacctata cccgcaaccg ccaaaacgcc agacctccct gaacgtcatt
     6841 aaaccgtgat gttaccgact ctctgactcg gaacagaatc agcgtcagag aaacggaaaa
     6901 cgcgatccag atcacaaatg cattgtattc acatcattaa ccgttttaag atcatttcat
     6961 cactttttcg caactcaccc gataatctgt tatgacaaca aacactgttt cccgcaaagt
     7021 ggcgtggcta cgggtcgtta cgctggcagt cgccgccttc atcttcaaca ccaccgaatt
     7081 tgtccctgtt ggcctgctct ctgacattgc gcaaagtttt cacatgcaaa ccgctcaggt
     7141 cggcatcatg ttgaccattt acgcatgggt agtagcgcta atgtcattgc cttttatgtt
     7201 aatgaccagt caggttgaac ggcgcaaatt actgatctgc ctgtttgtgg tgtttattgc
     7261 cagccacgta ctgtcgtttt tatcgtggag ctttaccgtt ctggtgatca gtcgcattgg
     7321 tgtggctttt gcacatgcga ttttctggtc gattacggcg tctctggcga tccgtatggc
     7381 tccggccggg aagcgggcac aggccttgag tttaattgcc accggtacag cactggcgat
     7441 ggtcttaggt ttaccgctcg ggcgcattgt ggggcagtat ttcggctggc gaatgacctt
     7501 cttcgcgatt ggtattgggg cgcttgttac ccttttgtgc ctgattaagt tacttccctt
     7561 actgcctagt gagcattccg gttcattgaa aagcctcccg ctattgttcc gccgcccggc
     7621 attgatgagc atttatttgt taactgtggt ggttgtcacc gcccattaca cggcatacag
     7681 ctatatcgag ccttttgtgc aaaacattgc gggattcagc gccaactttg ccacggcatt
     7741 actgttatta ctcggtggtg cgggcattat tggcagcgtg attttcggta aactgggtaa
     7801 tcaatatgcg tctgcgttgg taagtacggc gattgcgctg ttgctggtgt gtctggcact
     7861 gctgctacct gcggcgaaca gtgaaataca cctcggggtg ctgagtattt tctgggggat
     7921 cgcgatgatg atcatcgggc ttggtatgca ggttaaagtg ctggcgctgg caccagatgc
     7981 taccgacgtc gcgatggcgc tattctccgg catatttaat attggaatcg gggcgggtgc
     8041 gttggtaggt aatcaggtga gtttgcactg gtcaatgtcg atgattggtt atgtgggcgc
     8101 ggtgcctgct tttgctgcgt taatatggtc aatcattata tttcgccgct ggccagtgac
     8161 actcgaagaa cagacgcaat agttgaaagg cccattcggg ccttttttaa tggtacgttt
     8221 taatgatttc caggatgccg ttaataataa actgcacacc catacatacc agcaggaacc
     8281 ccatcaggcg ggagatcgct tcaatgccac ccttgcccac cagccgcata attgcgccgg
     8341 agctgcgtag gcttccccac aaaataaccg ccaccaggaa aaagatcagc ggcggcgcaa
     8401 ccatcagtac ccaatcagcg aaggttgaac tctgacgcac tgtggacgcc gagctaataa
     8461 tcatcgctat ggttcccgga ccggcggtac ttggcattgc cagtggtaca aaggcaatat
     8521 tggcactggg ttcatcttcc agctcttccg acttgctttt cgcctccggt gaatcaatcg
     8581 ctttctgttg cggaaaaagc atccgaaaac cgataaacgc gacgattaag ccgcctgcaa
     8641 ttcgcagacc gggaatcgaa atgccaaatg tatccatcac cagttgccca gcgtaatacg
     8701 ccaccatcat gatggcaaat acgtacaccg aggccatcaa cgactgacga ttacgttcgg
     8761 cactgttcat gttgcctgcc aggccaagaa atagcgcgac agttgttaat gggttagcta
     8821 acggcagcaa caccaccagc cccaggccaa ttgctttaaa caaatctaac attggtggtt
     8881 gttatcctgt gtatctgggt tatcagcgaa aagtataagg ggtaaacaag gataaagtgt
     8941 cactctttag ctaaccttgc atcgcattga ccaaaacttg aaccgattta gcaaaacgtg
     9001 gcatcggtca attcattcat ttgacttata cttgcctggg caatattacc ccctacaact
     9061 aattacttgc caaggcaact aatgtgaaaa gtaccagcga tctgttcaat gaaattattc
     9121 cattgggtcg cttaatccat atggttaatc agaagaaaga tcgtctgctt aacgagtatc
     9181 tgtctccgct ggatattacc gcggcacagt ttaaggtgct ctgctctatc cgctgcgcgg
     9241 cgtgtattac tccggttgaa ctgaaaaaag tgttgtcggt cgacctggga gcactgaccc
     9301 gtatgctgga tcgcctggtc tgtaaaggct gggttgaaag gttgccgaac ccgaatgata
     9361 agcgcggcgt actggtaaaa cttaccacca gcggcgcggc aatatgcgaa caatgccatc
     9421 aattagttgg ccaggacctg catcaagaat taacaaaaaa cctgacggcg gacgaagttg
     9481 caacacttga gcatttgctt aagaaagtcc tgccgtaaac aaaaaagagg tatgacgatg
     9541 tccagacgca atactgacgc tattaccatt catagcattt tggactggat cgaggacaac
     9601 ctggaatcgc cactgtcact ggagaaagtg tcagagcgtt cgggttactc caaatggcac
     9661 ctgcaacgga tgtttaaaaa agaaaccggt cattcattag gccaatacat ccgcagccgt
     9721 aagatgacgg aaatcgcgca aaagctgaag gaaagtaacg agccgatact ctatctggca
     9781 gaacgatatg gcttcgagtc gcaacaaact ctgacccgaa ccttcaaaaa ttactttgat
     9841 gttccgccgc ataaataccg gatgaccaat atgcagggcg aatcgcgctt tttacatcca
     9901 ttaaatcatt acaacagcta gttgataacg tgacaacgtc actgaggcaa tcatgaaacc
     9961 actttcatcc gcaatagcag ctgcgcttat tctcttttcc gcgcagagcg ttgcggaaca
    10021 aaccacgcag ccagttgtta cttcttgtgc caatgtcgtg gttgttcccc catcgcagga
    10081 acaaccaccg tttgatttaa atcacatggg tactggcagt gataagtcgg atgcgctcgg
    10141 cgtgccctat tataatcaac acgctatgta gtttgttctg gccccgacat ctcggggctt
    10201 attaacttcc cacctttacc gctttacgcc accgcaagcc aaatacattg atatacagcc
    10261 cggtcataat gagcaccgca cctaaaaatt gcagacccgt taagcgttca tccaacaata
    10321 gtgccgcact tgccagtccg actacgggca ccagtaacga taacggtgca acccgccagg
    10381 tttcatagcg tcccagtaac gtcccccaga tcccataacc aacaattgtc gccacaaacg
    10441 ccagatacat cagcgacaag atggtggtca tatcaatagt aaccagactg tgaatcatgg
    10501 atgcggaacc atcgagaatc agcgagacaa caaagaaggg aatgattggg attaaagcgc
    10561 tccagattac cagcgacatc accgccggac gcgttgagtg cgacatgatc tttttattga
    10621 agatgttgcc acacgcccaa ctaaatgctg cagccagggt caacataaag ccgagcatcg
    10681 ccacatgctg accgttcaga ctatcttcga ttaacaccag tacgccaaaa atcgctaagg
    10741 cgatccccgc caattgtttg ccatgcagtc gctccccgaa agtaaacgcg ccaagcacga
    10801 tagtaaaaaa cgcctgtgcc tgtaacacca gcgaagccag tccagcaggc ataccaaagt
    10861 taatggcgca gaacagaaag gcaaactgcg caaaactgat ggttaatcca taccccaaga
    10921 gcaaattcag tggtactttc ggtcgtgcaa caaaaaagat ggccggaaaa gcgaccagca
    10981 taaagcgcaa accggccagc atcagcggtg gcatgttatg aagccccact ttgatgacca
    11041 caaaatttag cccccatacg accactacca gtagcgccaa caccccatct tttcgcgaca
    11101 ttctaccgcc tctgaatttc atcttttgta agcaatcaac ttagctgaat ttacttttct
    11161 ttaacagttg attcgttagt cgccggttac gacggcatta atgcgcaaat aagtcgctat
    11221 acttcggatt tttgccatgc gtccgatggt taataacaac gacgaggcaa gaagggcgct
    11281 ggtagagcgt cgtagggata agttcatttg tctgtccggc agtgcgtttc gttatgtttt
    11341 attttagaga tgtaaagaaa tagcatggct acgctgccat ttatgaccat ttacttgagt
    11401 cgccagtaca gcctgagtgt cgatctaatc ggttatgcga tgacaattgc gctcactatt
    11461 ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata agttcgacaa gaaacgctat
    11521 atgttactgg caattaccgc cttcgccagc ggttttattg ccattccttt agtgaataac
    11581 gtgacgctgg ttgtgctctt ttttgccctc attaactgcg cctattctgt ttttgctacc
    11641 gtgctgaaag cctggtttgc cgacaatctt tcgtccacca gcaaaacgaa aatcttctca
    11701 atcaactaca ccatgctaaa cattggctgg accatcggtc cgccgctcgg cacgctgttg
    11761 gtgatgcaga gcatcaatct gcccttttgg ctggcagcta tctgttccgc gtttcccatg
    11821 cttttcattc aaatatgggt aaagcgcagc gagaaaatca tcgccacgga aacaggcagt
    11881 gtctggtcgc cgaaagtttt attacaagat aaagcactgt tgtggtttac ctgctctggt
    11941 tttctggctt cttttgtaag cggcgcattt gcttcatgca tttcacaata tgtgatggtg
    12001 attgctgatg gggattttgc cgaaaaggtg gtcgcggttg ttcttccggt gaatgctgcc
    12061 atggtggtta cgttgcaata ttccgtgggc cgccgactta acccagctaa catccgcgcg
    12121 ctgatgacag caggcaccct ctgtttcgtc atcggactgg tcggttttat tttttccggc
    12181 aacaacctgc tattgtgggg tatgtcagct gcggtattta ctgtcggtga aatcatttat
    12241 gcgccgggcg agtatatgtt gattgaccat attgcgccgc caggaatgaa agccagctat
    12301 ttttccgccc agtctttagg ctggcttggt gccgcgatta acccattagt gagtggcgta
    12361 gtgctaacca gcctgccgcc ttcctcgctg tttgtcatct tagcgttggt gatcattgct
    12421 gcgtgggtgc tgatgttaaa agggattcgt gcaagaccgt gggggcagcc cgcgctttgt
    12481 tgatttaagt cgaacacaat aaaaatttaa ttcagccttc gtttaggtta tctctgctaa
    12541 tatctttctc attgagatga aaattaaggt aagcgaggaa acacaccaca ccataaacgg
    12601 aggcaaataa tgctgggtaa tatgaatgtt tttatggccg tactgggaat aattttattt
    12661 tctggttttc tggccgcgta tttcagccac aaatgggatg actaatgaac ggagataatc
    12721 cctcacctaa ccggcccctt gttacagttg tgtacaaggg gcctgatttt tatgacggcg
    12781 aaaaaaaccg ccagtaaacc ggcggtgaat gcttgcatgg atagatttgt gttttgcttt
    12841 tacgctaaca ggcattttcc tgcactgata acggatcgtt gacacagtag catcagtttt
    12901 ctcaatg
//
LOCUS       DAETQL010000099        12736 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.099, whole genome shotgun sequence.
ACCESSION   DAETQL010000099 DAETQL010000000
VERSION     DAETQL010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.099"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1039
                     /locus_tag="K0W65_003310"
     CDS             <1..1039
                     /locus_tag="K0W65_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBI3096447.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1040..3205
                     /locus_tag="K0W65_003311"
     CDS             1040..3205
                     /locus_tag="K0W65_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HBI3096448.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKEADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3219..4130
                     /locus_tag="K0W65_003312"
     CDS             3219..4130
                     /locus_tag="K0W65_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HBI3096449.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4130..5425
                     /locus_tag="K0W65_003313"
     CDS             4130..5425
                     /locus_tag="K0W65_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HBI3096450.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5470..5700
                     /locus_tag="K0W65_003314"
     CDS             5470..5700
                     /locus_tag="K0W65_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096451.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5678..6178
                     /locus_tag="K0W65_003315"
     CDS             5678..6178
                     /locus_tag="K0W65_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HBI3096452.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6178..7596
                     /locus_tag="K0W65_003316"
     CDS             6178..7596
                     /locus_tag="K0W65_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HBI3096453.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7596..8444
                     /locus_tag="K0W65_003317"
     CDS             7596..8444
                     /locus_tag="K0W65_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096454.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8444..8899
                     /locus_tag="K0W65_003318"
     CDS             8444..8899
                     /locus_tag="K0W65_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HBI3096455.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8902..9594
                     /locus_tag="K0W65_003319"
     CDS             8902..9594
                     /locus_tag="K0W65_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HBI3096456.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9604..10935
                     /locus_tag="K0W65_003320"
     CDS             9604..10935
                     /locus_tag="K0W65_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HBI3096457.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10936..12666
                     /locus_tag="K0W65_003321"
     CDS             10936..12666
                     /locus_tag="K0W65_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBI3096458.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKRVEVA"
BASE COUNT         3437 a         3145 c         3355 g         2799 t
ORIGIN      
        1 tttctttccg aaccttgttg atcaccttct ggttaagcat cacacggctg atggcgttga
       61 agatggcatt tcaatctgct acttcaagcc atactcgcaa ggccgtgctc gctggcaggg
      121 tgacacaatc cacggcgtgt ggtttgacga agagccacca tacagcattt atggcgaagg
      181 tcttacccgt accaacaaat acgggcaatt ctcaattctg acgtttaccc cgctgatggg
      241 gatgtctgac gttgttacca agttcctgaa gaaccccagc aagtcgcaga aagtggtcaa
      301 catgaccatc tatgacgctg agcactatac cgacgagcag aaagagcaaa tcatcgcatc
      361 atatcccgag catgagagag aggcgcgtgc tcgcggtatt cctacgatgg gtagtggtcg
      421 aatcttccag ataccggaag agacgattaa gtgtcagccg ttcgagtgtc ctgatcactt
      481 ctacgtaatt ggcgggatgg atttcggatg ggatcaccca caggcgcaag ttcagctttg
      541 gtgggataag gaggcagaca caatctacgt ttcacgcgtg tggaaggcga aagaaaaaac
      601 agccgttcag gcgtggggag ccgttaaatc atgggcgcat aaagtgccaa ccgcatggcc
      661 tcatgatgga aaccagcacg agaagggcgg cggtgagcag ctcaaagggc agtatgcaga
      721 cgctggattt atgatgttgc aggagcatgc gacatggcct gatggcggta acgctgttga
      781 gccaggcatc actgaattgc gcgacatgat gctcgatggt cgcttcaaag tattcaatac
      841 ctgtgagcca ttctttgagg agttccgcct ctatcaccgt gatgaaaacg ggaaaatcgt
      901 caagcttaac gacgacgtgc tatccgccgt tcgctatgca tacatgatgc gccgcttcgc
      961 aaaaatgatg cgcgacatca aaaaaccaaa agagaaaaag ataccagccc caatcaggcc
     1021 catcgcacgg agaacttaaa tggccgacga aaacagactc aattccattc tgtgtaagtt
     1081 tgacgcagac tggatggcga gcgatgaagc cagaaccgag gcgacaaatg acctgtattt
     1141 tagccgagtg tcgcaatggg atgactggct atcaaactac actaccctgc aatatcgcgg
     1201 acaattcgat gttgttcgcc cggaggtcag gaaactggtc gcagagatgc gccggaaccc
     1261 tgtcgacgtt ctcttccgac ccaaagacgg cgctaatcct gatgcagccg atgtattgat
     1321 ggggatgtat cgtactgata tgcgccataa cacggcaaag attgccgtta acgttggcgt
     1381 tcgtgagcag atagagtcag gcgttggtgc atggcgtctg gtcacgcagt acgaagacaa
     1441 cgatccaaca agcaacaatc aggtaatccg acgcctgcca atccatgaag cctgctcaca
     1501 cgtcatatgg gacgccaaca gcaagcagat ggataagagc gacgctaagc actgcacggt
     1561 gattaacgcc ttgtcgcgca atggctggaa agagttcgca gaggattacg gtattgaccc
     1621 ggataccctg ccatctttcc agaatcctaa cgatacatgg ctattcccgt gggtgtcgaa
     1681 tgatgtcgtc tacgtcgctg agtattacga ggtagaagag gagaaggaga aggtcttcat
     1741 ctaccgcgac ccgctgacag gtgagccggt cagctattac cagcaggata tcaaagacgt
     1801 catcgacgac ctggctaatc gtggattcat taaggtagca gagcgcaagg tgaagcgtcg
     1861 gcgtgtgtat aagtcgatca tcacctgtac gcagatactg aaagaccgcg agaagatagc
     1921 cggagagcat attccaatcg ttccagtgta tggcgaatgg tcattcgctg gtgacaagga
     1981 gtgctacgag ggtgtggtaa ggctgacgaa agacggtcaa cgccttcgta acatgattat
     2041 gtcgttcaac gccgatattg ttgctcggtc accgaagaag aaaccgacct tcttccctga
     2101 gcaaatcgaa ggctacgaat acatgtacgg cggaaatgat gactatccgt actatctgca
     2161 gaacaggact gatgaaaacg gtaacgacct gccgattggt ccaatctcct acatggaaaa
     2221 ccctgaagtg ccgcaagcca acgcttacat gcttgaggct gccaccaacg cagtgaaaga
     2281 agtggctagt cttggcgtgg atgcgcaggc ggcaaatggt caggtcgctt ttgataccgt
     2341 caatcaactg aacatgcggg cagaccttga gacatacgtg tttcaggata acctggctac
     2401 cgcaatgcga cgtgatggcg agatttatgc ctcaatggtc aacgatattt atgacgttcc
     2461 tcgtcatgta acgctgacac ttgaagatgg aagcgagaaa gacgttcaac tctatgcgca
     2521 agttgtcgat taccagtccg gcaatgtggt cacactcaac gacattcgcg gtcgatatga
     2581 gtgctataca gacgttgggc catccttcca gagtatgaag gaacagaacc gcgcagagat
     2641 tcaggagttg ctcaccaagg ttccgcaagg tactccagag ttccagatgc tgatgctgca
     2701 atacttcacg ctgcttgacg gtaaaggcgt cgagatgatg cgagagtacg cgaacaagca
     2761 actggtgatg atggggctga agaaaccaga aacacctgaa gagatggaga tggtgcagca
     2821 ggcacaacaa cagccgcagc agccatcagc agagcaaatt caggcgcagg gtatccttct
     2881 gcaaggtcag gctgaattgc tcaaggcaga gaaccaacag gcgcagattc aggttgaagc
     2941 tgccaaggtt gaagcccaaa accaactcaa cgccgcgaag attgcagaaa tattcaacaa
     3001 tatggacctc gacaagcagg cagaactgcg tgagtacctc aggctcgtag gtcaattcca
     3061 gcaacagcgc agcaaagatg ctcgtgctaa cgctgagctg cttcttaaag aagcagacca
     3121 gactcattca caacgcatgg atttcgcgaa tcttatgcgt caagttcaaa tcccctccgg
     3181 cggagtagcc gagacacctc aataagagag agttaatcat ggaccaaacc accgacattc
     3241 aggcttctga agaattaacc ctgcccggca atcatgcagc ggcatctgct gatggcttag
     3301 ttgtcgataa tgccaacgac agcgcaggtc aggaagaagg ctttgagatt gtccggaaag
     3361 acgatgagaa accaaaacaa gacccggcaa ctaatgctga atttgcccgt cgccgcatcg
     3421 aacgcaaacg ccagcgtgag cttgagcagc agatggaagc ggttaagcgt ggagagttgc
     3481 cggagcacct gcgggtgaac cctgagttac caaaacaacc agaccctaac gattatcttt
     3541 ccgaagatgc actggctaag tacgactatg accagagccg cgcactggct gccttccagc
     3601 aggcaaacag tgaatggcag atcaaggcta tggacgcacg aagccaggct gtcgccgagc
     3661 agggtcgcaa aactcaggag ttcacccagc aatcagcgca atacgtcgag gcggcccgta
     3721 agcactacga ggcagcggaa aagctcaata tccctgacta tcaggagaaa gaggatgcat
     3781 tcatgcaact ggtgccgcca gcagtcggtg ccgacatcat gcgcctcttc ccggagaaat
     3841 ccgccgctct catgtatcac cttggtgcta atcctgagaa aacacgccag ttgctggcga
     3901 tggacgggca atccgcgctg attgaactca ctcgactgtc agaacgttta actctcaagc
     3961 ctcgagccaa gcctgtttca gaagccccgt tacctgatga acccattcag ggacatgctg
     4021 ttgctgcaaa tatctctgcg attgaaaagc agatggaggc ggcagcaaac aaaggggatg
     4081 tagagacgta ccgcaagctc aaggcgcaac tgaataaagg aattcgataa tggcattaaa
     4141 tgaaggtcaa ctggtcacgt atgctctgga tgaaatcatc ggaaccgtcc agaacctgac
     4201 gccaatggcg tcaaaagtga caaaataccc ccctccggca gaatccatgc agcgttcaag
     4261 caacaccgtg tggatgcctg ttgagcagga agcgccaacc cagactggct gggatttaac
     4321 tggcaacgca accgggattc tggaactctc cgtgaagtgc aacatgggcg atccggataa
     4381 cgatttcttc gagcttcgtg cagatgacct gcgtgatgag cgttcttacc gtcgccgcat
     4441 ccaggcatcc gccaaaaaac tggcgaataa cattgagtca gcgattgcca aacaggcaac
     4501 tgaaatgggc tcgcttgttg ttcacgatac ccgcgcaatt ggtccatcga ctggcctgcc
     4561 tggctgggat tttgtgtctg atgcagagcg actgatgttc tcccgtgagc taaaccgcga
     4621 catgggcatc agttacttcc tgaaccctga cgattaccgc aaagcaggcc gcaacctggt
     4681 agatggtgac atcttcgggc gcgttcctga agaagcatat cgaaacggta ctattcagcg
     4741 tcagattgct ggctttgatg aaattcttcg ctcaccgaaa cttccggcag ttaccaagtc
     4801 aaccgcgact ggtgtaactg tttctggtgc gcagaagttt aagccgcagg catacaccct
     4861 tgataccgat ggtaacaaag agaacgtcga caaccgtgtt gcaacggtga ccgtatcctc
     4921 caccaccgga tttaagcgcg gcgacaaaat cagcttcatt ggtgtgaagt tcctgtctca
     4981 gatggcgaag aatgtgctga ctgatgacgc gactttctca atcacccgtg tgatcgatgg
     5041 tactcacatc gaaatcacgc cgaagccgat tgcgctggat gacgcgtcac tgacaaaaga
     5101 agagaaggct tacgctaacg taaacacctc tcttgctgat accactccgg taaacgttct
     5161 gaacgtggca acaaccaccg ctaacgtgtt ctgggctgat gactcaatcc gcctgctgtc
     5221 tcagccgatc ccggtaaccc atgaactgtt tgctggcatg aaaacgtctt ccttcagcat
     5281 tccaggtatt ggtgttaacg gcatcttcgc aacgcagggt gatatcaaca ctctgtctgg
     5341 taagtgccgt attgctgtgt ggtattcagc atgtgctgta cgaccagagg caattggtgt
     5401 tggtctgcct aaccagaccg cgtgataacc agagggagct tcggctcccg tttttatctg
     5461 gagacaagca tgacacacat gatctttcgt catggcgaca tgaagaagtg gaaaggcgtt
     5521 ggctacgact ttgaaatcgt gaaagccgaa gagcttcagg aatatctgga tgctggctgg
     5581 ttttcacatc ctgatgacct tttgaaggat gttgcagagc cagagccaga agaaaaacag
     5641 cgtaaaaagc ctggtcgaaa acctaaggcg gcagcagatg aacctgacaa cgaagggtga
     5701 tttagttctt gcggcattac gtaagctcgg tgttgcatca aatgccacgt taaccgatgt
     5761 cgaaccgcag tctatggaag acggcgtcaa cgaccttgaa atgatgatgg ctgaatggct
     5821 tggcggtgat gcgtcacctg gtatcaacgt tggctacatt tttgctgatg cagatgttgc
     5881 tccggatccg ggcgatgagc acggtttatc aaataacgct atcaatgccg tcattttcaa
     5941 ccttgcctgc cgaattgctc cagattatgc gctggaagcg tctgcaaaac ttataaccac
     6001 tgccagatac gggaaagagc gactcgtcaa actgtctgca atggacaggg caaaagcagc
     6061 taaatgtaag tccggttatc caaaccgtat gcctgttggt agtggtaacc agttggcgaa
     6121 gtggaatggt tggaattact tccaccgaaa ggaaccttgc gataacggga gcgaataatg
     6181 cctattcagc aacttccgct tatgaaaggt gtcggcaaag acttccgaaa cgccgactat
     6241 atcgactatc tgccagtgaa tatgctggct acacccaaag aaatcctgaa cagcagcgga
     6301 tatcttcgct cattcccggg cattgccaaa cgttctgacg tgaacggtgt atctcgcggc
     6361 gtcgagtaca acatggcgca gaatgctgtt tatcgcgtgt gtggcggcaa gctgtacaaa
     6421 ggagaaagtg aagtcggtga tgttgccgga agtggtcgtg tatcaatggc gcatggtcgg
     6481 acatcacagg cggtaggcgt taatggtcaa ctggtcgagt atcgctatga tggcacggtc
     6541 aaaaccgtct caaactggcc tacagacagt ggattcactc agtatgagtt aggttcagtt
     6601 cgcgacatta cgcgcttacg tgggcgttat gcgtggtcaa aagacggaac tgattcatgg
     6661 tttatcactg accttgaaga cgaatctcat cctgaccgtt acagcgcaca atatcgtgcc
     6721 gagtctcagc ctgacggcat catcggtatc gggacatggc gagacttcat cgtctgcttt
     6781 ggttcatcga ctattgaata tttctccctg actggcgcaa ccaccgttgg tgccgctttg
     6841 tatgttgcac aaccatcgct gatggtgcag aaaggcattg ccggaactta ctgcaaaacg
     6901 ccattcgctg attcgtatgc gtttatcagc aatccggcaa cgggtgcgcc gtctgtgtat
     6961 atcatcgact ccggtcaggt gtcaccaatt gccagcgcga gcattgagaa aatcctccgc
     7021 tcctacactg ctgatgaact ggctgatggt gtgatggaat cgctgcgatt tgatgctcat
     7081 gagttgctga ttatccacct tccgcgtcac gtcctcgtgt acgacgcatc ttcaatcgcc
     7141 aatggcccac aatggtgtgt gctgaaaaca ggtctgtatg acgatgtgta ccgcgctatc
     7201 gacttcgttt acgaaggcaa tcagataacg tgcggcgata agctggaatc tgttaccggg
     7261 aaactgcaat tcgatatcag cagccagtac gacaagcaac aggaacacct gctgtttact
     7321 ccactcttca aagcggataa cgccagagtg ttcgaccttg aggttgaatc gtcaactggc
     7381 gttgcgcagt acgccgaccg cctttttctc tctgcaacca ctgactgcat caattacggg
     7441 cgtgagcaga tgattgagca gaatgaaccg ttcgtttacg acaagcgtgt tttgtggaag
     7501 cgagtagggc gcatcaggaa aaatgtcggc ttcaaattgc gcgttatcac gaagtcacct
     7561 gtcactctgt ctggcgctca gataaggatt gagtaatggc tgattcgaat ctcaatgtgc
     7621 cggtaatcat ccaagctacg cggctcgata catcagtcct tccacgcaat atcttctcgc
     7681 agtcgtatct gctttacgtt atcgcacagg gcactgatgt tggtaacgtg gctaacaaag
     7741 ccaacgaggc cggacagggc gcttatgatg cacaggtcag gaacgatgag caggatgtga
     7801 ttctcgctga ccatgagcag cgaatttctg ctgcggaagc aacgcttgtt aatcatgagg
     7861 agcgaatcag acaggcagaa tcaactcttc aggaccatga aacacgaata gctcagaatg
     7921 aaagcgatat ttcgtcgctt gacacaagag ttcagtcgct ggagtcgcag gtttcagacc
     7981 atgaaacgcg catcgatgct ctggagtatg caaccacacg caagaagtca gaggttgttt
     8041 actctggtgt atctgtaacc atcccgacag cgccgaccaa ccttgttagc ctgctgaaaa
     8101 cgctcacacc gtcatccggc tcgttggcac cattcttcga caccgttaac aacaagatgg
     8161 ttgtgttcaa cgagaacaaa accctgctct tcaagttgtc gattgtcggc acgtggccca
     8221 gcggaaccgc caacaggtca atgcagctaa ccttttccgg ctctgttcct gacacactgg
     8281 tcagcagtcg taatgcggcg acaacaaccg acaacatcct gttagctacg ttcttcagcg
     8341 tggataaaga cggctttctt gccacaaatg gcagtacgtt aaccattcag tcgaatggtg
     8401 cggcgtttac tgccacaacc atcaagataa tcgcggagca gtgatgatta cattcaaacc
     8461 aacgcgaaac atcgacctga tagaagcagt cggaaatcac cctgacatta tcgccgggag
     8521 caacaacggt gatggatacg actacaaacc tgaatgccgt tactttgagg tgaacgtgca
     8581 cggtcagttt ggcggcattg tttactatca ggagattcag ccgctgacat tagattgcca
     8641 cgccatgtac ctgccagaga ttcgcggctt cagcaaggaa attgggctgg cgttctggcg
     8701 atacattctg actaacacca ccgttcagtg cgtaacatcg ttcgctgcac gcaaattccg
     8761 acacggtcag atgtactgcg caatgattgg ccttaagcgt gtaggaacca tcaagaaata
     8821 cttcaaaggc gtggatgacg tgacttttta cagcgccaca cgcgaagaac taatcgactt
     8881 cctgaatcac gggagatagc catgttatat gcatttaagc tgggcagaaa actgcgcggc
     8941 gaggaacctt attgccctga aaaaggcggg aaaggtggca gttctgataa aagcgcaaag
     9001 tatgcagcag aagcccagaa gtatgctgca gacctgcaaa atcagcagtg gcagacgatc
     9061 atgaaaaacc ttgctccgtt cacgcctctt gcggagcagt atgttaacca gcttcagaac
     9121 ctttccagtt tagaaggtca ggggcaggca cttaatcagt attacaactc taagcagtat
     9181 aaagaccttg cagggcaggc gcgttaccag agtcttgctg ctgcggaggc gacgggtgga
     9241 cttggttcga cagccacaag caatcaactg gctacgatcg cgccgacact cggtcagtct
     9301 tggttatcaa atcagatgag caattacaac aatctggcaa acgttgggct tggtgcgctg
     9361 caaggtcagg caaacgccgg gcagacgtac gccaacaaca tgagcagcat tgcacagcaa
     9421 agcgcagctc ttgccgctgc taatgccaat aaaccatcaa gtcttcagac tgcaattagc
     9481 ggtggcacgt ctggtgcgat tgccggtgca ggtcttgcca gccttttggg aacatcaacg
     9541 ccttggggcg ctggtattgg tgctggtatc ggattgcttg gctcgttgtt ttaaggggga
     9601 atcatggcta cttggcaagg aacaaacggc ggattgttgg ctggtatcgg cggcgtcaac
     9661 tcaaacgctc cgagcgtaaa tgacatcggc aatacgcttc agcttatcag gcagaacaat
     9721 gatattgagc gttcaggcgc taacaatgtt gggctgactg ctttgcaagg tctttcaggt
     9781 attgcgggtg tttttcagca ggaaaagcag gctcagcggc agaaagaatt tcagcaggcg
     9841 tacgctaatg cttatgcgtc tggtgatcgc ggtgctttgc gtcagttggc tactcaatat
     9901 ccagaccaga ttgaatccgt tcgtaaaggc atgggattca ttgatgaaga gcagcgtaat
     9961 tctatcggca ccttagcggc tggcgcacgc cttgcgtcat cgtctccaga agcaatgcaa
    10021 tcatggctgc aaaacaacgc cggtgagtta gctcgtgttg gcgttaatcc tcatgacgtc
    10081 gctcagatgt accaacagaa cccgcggcag ttcggcgaat ttgtcgatca cctggggatg
    10141 aacagtctcg ggcccgaaaa atactttgac ctacaggata aaatgcaggg tcgccaggtt
    10201 accatgcgcg gtcaggatct ggattcgcaa accgccgctc ggaatcaggc aatcacaatg
    10261 cgcggacaag atatccaggc gaatttaggt cagcagcgca ttaatctgga cgcagaaaca
    10321 aaccgcatta acaacgaaaa taagcgcctt gaccggatgc tatcagcaga aactaacgac
    10381 ctgaagcgcc aggaaataca gagccgcata gcagccaaca accagcagtt gcagcagaag
    10441 cagcaagcgc taaatgatgg ctacaaagac ggcatcaaca ccctcacaac cagcatgttc
    10501 actctgaacg atatcgttag ttctccttca cttaagagca ttacaggctt acgtggagta
    10561 atccccaacg ttccaggctc acaggctgca gacactcagg cacgacttga tacctttaaa
    10621 tcacaagcat acctgacagc ggttcaggcc atgcgaggca tgggcgcact ttctgatgcc
    10681 gagggcaaaa agctcgacca ggctgttggt tcgctgcaga actcgcagag cgaggagtcc
    10741 tttcgtcgca acgctggcgt catcctgaac acgctcaacc agaagcgtaa tgaggcggtt
    10801 ggtaagtacg ttcagcaaaa cggtatcaag cgagtggaag cgcctcaggc ttctatagat
    10861 tacctgaagc agcaccccga gctgtcaatc gacttcatta atcgctacgg atatcttcca
    10921 tctttggggc agtaaatggc taattaccgt gatttgttag agcaggctgg cgcacgttac
    10981 ggtgtgccag aagggttgat gactgcactg ggtgccaagg agtcttctta caaccctgcc
    11041 gcagtaagct ccgccggggc tgtaggattg actcaggtca tgcctgggac atggcgtgat
    11101 atgggttata ccgatgagca aatgcaaaac cccgaatatc aggctgacgc tggcgcgcgc
    11161 tatctggcaa agatgtacca gcagtttggt aactggcgtg actctcttca ggcttatcac
    11221 gacggtcccg gcaacgttat gaaggcaaag cgtggtgaat atacgccagg acctgaaggc
    11281 cgcggttacg ttgatgatcg ctttgctcaa tgggctggtg acccggtgac agactcaaca
    11341 gtcgaacagc gcgccacctc tgcaaaggta catcctcagc aagaccctaa caacccgttt
    11401 gcacaactgg aagtacagtc atccgaacaa gtatcggcat caggtgtgca gtcagaccca
    11461 aataatccat ttgctcagat tgagcagcag gcagccagtc agcagccacc tcaacccgta
    11521 agttctgtcg caccgaaacc tgttcagcaa caaacagtta atcaggccaa taatgcacca
    11581 acacgggaag aaccatcatt gatgcaacaa gctggcgatt ggctcactgg tggtcaaagt
    11641 gcagggcaaa ttgcagaaca ggctggtcgt ggtctggtaa acataccatt tgacgtattg
    11701 cagggtggcg caagtctgat taatgcaatc agtcaggggc ttggggggcc aaaagtattg
    11761 gatgatgttt atcgtccagt agacagaccg acagacccct acgcgcaagc tggagagtca
    11821 ataggcggtt atcttgttcc tggagcaggt gtagctggaa acatggtcat tggttctctc
    11881 gctgacgcgg cgaatcaacg gggtgatttt gccgaaaatg ccgctattaa tgccggactt
    11941 agcattgcta cgcatggcct gataaatggc gttacccgtg gtgttcgtgg tgcatcaaat
    12001 ataattagtg gcaataaaac atctgcacag agagcgacca ctgcgccaac agaaacatca
    12061 ccattctccg gtgatgccgc tgcagcaaca aatcctgcgg ttcatgccgc agaggcaaga
    12121 gtagcacaag gtgtaccaat gacgcctgcg acgaagaacc cagaggaagt cgttcgcaca
    12181 gtagcagcac aaaaaaggcc aaatctcgct tcatcgcttg atgaactaga tatcaatcct
    12241 caggctgaag ttctggagtc tgctgaaagg cttaatgttg attcattact cccttcacac
    12301 ttttccggga acgagcaata caaggcagtt gagcaagcaa tcaagtcccg tgcgggttct
    12361 gctctacagg tgcaggaaaa tgaagcaatc aggcagctag cacagggcgc gggtgagata
    12421 attgatcgcg tttccggtgc aaaagatgct cttggtatga gcgacaagtt tattgatacg
    12481 gtcaatggaa gaatgtctgc gctgatgaaa cgaagcgacc agctttatcg caatgttgaa
    12541 aaggcgatgc ctgcaggtgc aaaaattgat gcgccatcaa caaggtcaat gctcaaacag
    12601 gtggcagaag atcttggcgg gatgaaaaac cttgacccta ttgaaaagag agttgaggta
    12661 gcctgagttt aacggacact ccttcctgaa atagaatggc atcagaagga gctaataatg
    12721 agcagaaaaa cccaac
//
LOCUS       DAETQL010000100        12695 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.100, whole genome shotgun sequence.
ACCESSION   DAETQL010000100 DAETQL010000000
VERSION     DAETQL010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.100"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="K0W65_003322"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="K0W65_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBI3096459.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="K0W65_003323"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="K0W65_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HBI3096460.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="K0W65_003324"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="K0W65_003324"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBI3096461.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="K0W65_003325"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="K0W65_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBI3096462.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="K0W65_003326"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="K0W65_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBI3096463.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="K0W65_003327"
     CDS             4025..4615
                     /locus_tag="K0W65_003327"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBI3096464.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQSAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="K0W65_003328"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="K0W65_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBI3096465.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="K0W65_003329"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="K0W65_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HBI3096466.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="K0W65_003330"
     CDS             complement(6551..6658)
                     /locus_tag="K0W65_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096467.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="K0W65_003331"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="K0W65_003331"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBI3096468.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="K0W65_003332"
     CDS             7648..7875
                     /locus_tag="K0W65_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HBI3096469.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="K0W65_003333"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="K0W65_003333"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBI3096470.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="K0W65_003334"
     CDS             complement(9447..10703)
                     /locus_tag="K0W65_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HBI3096471.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="K0W65_003335"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="K0W65_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBI3096472.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="K0W65_003336"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="K0W65_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBI3096473.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="K0W65_003337"
     CDS             complement(12282..>12695)
                     /gene="torD"
                     /locus_tag="K0W65_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBI3096474.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3032 a         3241 c         3197 g         3225 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcagtc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 agcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa tatccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc attatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 gactctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg tggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgca gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcggtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgt
//
LOCUS       DAETQL010000101        12662 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.101, whole genome shotgun sequence.
ACCESSION   DAETQL010000101 DAETQL010000000
VERSION     DAETQL010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.101"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            128..1825
                     /gene="maeA"
                     /locus_tag="K0W65_003338"
     CDS             128..1825
                     /gene="maeA"
                     /locus_tag="K0W65_003338"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBI3096475.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1959..2969
                     /gene="adhP"
                     /locus_tag="K0W65_003339"
     CDS             1959..2969
                     /gene="adhP"
                     /locus_tag="K0W65_003339"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HBI3096476.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3115..3399
                     /locus_tag="K0W65_003340"
     CDS             3115..3399
                     /locus_tag="K0W65_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBI3096477.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="K0W65_003341"
     CDS             complement(3627..4280)
                     /gene="fdnI"
                     /locus_tag="K0W65_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HBI3096478.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="K0W65_003342"
     CDS             complement(4273..5157)
                     /gene="fdnH"
                     /locus_tag="K0W65_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HBI3096479.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="K0W65_003343"
     CDS             complement(5170..8217)
                     /gene="fdnG"
                     /locus_tag="K0W65_003343"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7630..7632),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBI3096480.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8449..9330
                     /gene="yddG"
                     /locus_tag="K0W65_003344"
     CDS             8449..9330
                     /gene="yddG"
                     /locus_tag="K0W65_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBI3096481.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9585..10685
                     /gene="ompC"
                     /locus_tag="K0W65_003345"
     CDS             9585..10685
                     /gene="ompC"
                     /locus_tag="K0W65_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBI3096482.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10849..11334
                     /locus_tag="K0W65_003346"
     CDS             10849..11334
                     /locus_tag="K0W65_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBI3096483.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11444..12433
                     /locus_tag="K0W65_003347"
     CDS             11444..12433
                     /locus_tag="K0W65_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBI3096484.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3341 a         3129 c         2941 g         3251 t
ORIGIN      
        1 acgattaaac accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac
       61 gccctggcgg taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt
      121 gagtgacatg gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc
      181 tgtactgctg gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg
      241 taacttcaac ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga
      301 acgagcatgg atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg
      361 taacatccag gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga
      421 gatgatgcct gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat
      481 ctaccgccgt tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat
      541 tctgcaaaac gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat
      601 tctggggctt ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct
      661 ctataccgcc tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg
      721 aacgaacaac caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat
      781 cactgacgat gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg
      841 gccggacgtg ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa
      901 ccgctatcgc aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac
      961 agtcggcaca ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat
     1021 cgtcttcctt ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac
     1081 ccagcgcgaa ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt
     1141 tggcctgctg acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa
     1201 gcgcgaaaac ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt
     1261 gcgcaatgta aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga
     1321 agagatcatc cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa
     1381 cccgacgtct cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc
     1441 gctggtcgcc actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat
     1501 cgcccagtgt aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg
     1561 cgcgtcacgt atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc
     1621 gccgctggtc ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt
     1681 ctcccgcgca attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa
     1741 aacgtctgct gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg
     1801 cgactaccgc cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt
     1861 ccctctccct ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct
     1921 cacctgctat gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga
     1981 aggatcatca tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc
     2041 tgctgaaaat ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt
     2101 ttggtgacaa aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg
     2161 gcccaggtgt cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag
     2221 gatgcggtca ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa
     2281 atgccggata cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg
     2341 cggtaaaagt gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg
     2401 tcaccaccta caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct
     2461 acggtcttgg cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca
     2521 aagtgatcgc cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag
     2581 atttagcgat taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg
     2641 gcgctcacgc tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg
     2701 ctgtccgtgc aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg
     2761 atatcccacg tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc
     2821 aggatctaac cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc
     2881 tgcgtccgtt agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg
     2941 gccgtatggt gattgatttc cgtcactaag aggtctttgc tgcgaccccc atgttcgggt
     3001 cgcagcatcg cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg
     3061 aacaataatg atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa
     3121 atggccaatc atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc
     3181 agcctgcgcg agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg
     3241 accggaaaag cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt
     3301 tcgccgcaaa tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg
     3361 gtggttgtgt cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg
     3421 ccatttgaaa ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca
     3481 cgttcacttc aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc
     3541 atccgggatg aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc
     3601 attttcagct taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct
     3661 gccttctcga tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc
     3721 ttcccttcga tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc
     3781 aggatggcgt ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca
     3841 acctgcatcg ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc
     3901 agcacgaaaa tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta
     3961 ccaacatccg ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac
     4021 ggaatatctt tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt
     4081 gcgacgaaaa tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg
     4141 aaggtttgcg tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc
     4201 aggaagaagc aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca
     4261 atcattttcg acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc
     4321 aataccgatg tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag
     4381 cggtttcaac gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag
     4441 accgtgatac agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc
     4501 gaccccttcc gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac
     4561 gcgctgttcc gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg
     4621 acaggttttc acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca
     4681 tttatatacc cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat
     4741 gcagtaacca cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg
     4801 aatgattgca ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca
     4861 gccgtcttta cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat
     4921 caccgtccag gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc
     4981 cacttcatca cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat
     5041 acaggtggaa acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg
     5101 cggcgtgatg gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg
     5161 ttcgccccct tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga
     5221 gtttgcatca ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc
     5281 ctcaaagccc cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag
     5341 cggtttcaga cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac
     5401 agtgacacga tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac
     5461 aaactgttcc ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg
     5521 ctcggtcaga cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag
     5581 cgcgtcttgt tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt
     5641 agtgcccagc ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt
     5701 gttgatggca aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt
     5761 acccggtgcg gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca
     5821 ctggatcagc atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt
     5881 gtagagtacg cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc
     5941 ggacgggtcg gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt
     6001 gtagatccag caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag
     6061 caactgacct ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata
     6121 gccgttgttc tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca
     6181 gctcatcttc atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag
     6241 atgatggtag atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg
     6301 acctttccag tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc
     6361 aaagcaggtc gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt
     6421 cgactcgccg tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac
     6481 catgtacttc agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac
     6541 cgggttaaag ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata
     6601 cttgatgacg tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc
     6661 tttctgcgcg gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt
     6721 gctccagtag ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga
     6781 ctgcaaatca acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag
     6841 cagacctaag tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc
     6901 accggccata cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg
     6961 cgcacccacg gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg
     7021 tgcgctggtg gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca
     7081 gatgttttca acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac
     7141 acagcgcgga tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata
     7201 gttccaggac gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc
     7261 ttcgaaagca aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc
     7321 ggcgttgatt ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc
     7381 cgtaccggaa cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg
     7441 atcgacaacg atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc
     7501 gacgggatgc gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat
     7561 atccacccag tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     7621 tggtccgtgt cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa
     7681 tttctgggtc agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga
     7741 aagccaacgg tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc
     7801 agctttcatc agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt
     7861 gtcagaacct ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc
     7921 cagcaaaccg gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc
     7981 ttcaatgtga taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa
     8041 tagcccgcaa cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa
     8101 tttgtagttt cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt
     8161 tgttccagcc ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc
     8221 tttcctcttt tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt
     8281 tttgcgcgaa atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga
     8341 cctacctcaa tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct
     8401 ataaaattca gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa
     8461 aaagcaacgc tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt
     8521 cgcggtgtca gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc
     8581 gggctgctgt taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat
     8641 ttactcgccg ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg
     8701 tatgcggcga cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc
     8761 ctgacaattc tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca
     8821 ggattattat tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat
     8881 tatgatgaaa tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt
     8941 ggtgcgttta tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac
     9001 ggaattaccg tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg
     9061 ccacaaccag aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt
     9121 accttaggat ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata
     9181 atggcggtag gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc
     9241 agcgccccgc tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg
     9301 ctctgctggc tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt
     9361 aatcagataa attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat
     9421 atctcatttc gacttcgatc acatatcgat aaataatatt attctatttt tctcattgtt
     9481 tttaatcacc attctatttt aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt
     9541 cgtttagaaa atacgtttaa cgataattaa aaggattctg ttaaatgaaa ttaaaaatag
     9601 ttgcggtggt tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata
     9661 acaaggatgg taataaactc gacctttatg gcaaggttac cgctctacgt tattttactg
     9721 atgataagcg tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc
     9781 aaatcaatga tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg
     9841 atgaagccaa cggctcgcgc ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta
     9901 gtgaatttgg ctctctggac tatggtcgta actacggtgt cggctacgac attggttcat
     9961 ggaccgatat gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga
    10021 cataccgtac caccggtcta gcaacctatc gcaactacga tttctttggc ttaattgaag
    10081 ggctgaactt tgccgcgcaa tatcaaggca aaaataaacg tactgacaac agtcatcttt
    10141 atggtgctga cgacacgcgt gccaacggtg acggtttcgg tatctcctca acttatgttt
    10201 atgatggctt tggtatcggt gcggtatata ccaaatccga tcggacaaat gcgcaggaaa
    10261 gagccgcagc taatcctctc aatgcctccg gtaagaatgc agaactatgg gccacaggta
    10321 taaagtatga tgccaacaac atctactttg cagctaatta cgatgaaaca ttaaacatga
    10381 ccacctatgg ggatggttat atctctaaca aagcacaaag ttttgaagtg gtggcgcaat
    10441 atcaattcga cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc
    10501 tgggccgcta cgccgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct
    10561 tcaacaaaaa tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact
    10621 ttacccgtgc cgtagatatt cgcaccgata acatcgtcgc tacgggcatt acctatcagt
    10681 tctaacgtcc tctcctgacc agcccttctc tgggctggtt tttgatttaa gtgctgctgt
    10741 atgactcctt aagtaaaaat gagcaattct tgatatttct ggtcattcac ctcaattaat
    10801 attattccat atcctccggg gacatcttac tataatattt attgatgagt gattatgacc
    10861 aatattaata cagcttgtgt aaaaaataat gccagttatc aacttaataa cgcattacct
    10921 aacaaagaaa ccatctccag caatttttgt gaacgactag aacaatgggg taataaatcg
    10981 gttaataatg gtgaagaaag agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg
    11041 aacatggcat ctcttgattt gtcatatctt gatttgagtg aattaccgcc tatcccctcg
    11101 acagttaata cgttaaatct ggaaaacaat tgtctaactt gtcttgactt agctgacaat
    11161 gccagtctcg ttaatatcaa cctcagcgtt aataaaatta acacgataac ctttccaaaa
    11221 gaaccaaaac tggaaaacat ttatattaat cataacaatc tggaaagttt agatttaaaa
    11281 aatcagcatt cattggttaa tctggaagcg caaaataata gcctaaaaaa atgatatttc
    11341 tgatagttat aaactgaaat ttcttaatct tgattataac aagctagcat cgctggatgt
    11401 ctcccggcaa gaatctctga ttgagttaag tgcccatcac aatatgatca ctgaccttat
    11461 attacacaat catcccagga tgaaaacaat cactttaaac gacaaccata ttgcacattt
    11521 aaacgccaaa aacactacaa aactggaata tttaaactta agcaataaca atttactgcc
    11581 aaccaatgac attgatcaac taatatcatc aaaacatctt tggcatgtat tagttaacgg
    11641 catcaacaat gatccacttg cacaaatgca gtactggact gcagtaagaa atataattga
    11701 tgacactaat gaagtgacca ttgatttatc aggacttaat ttaaccactc aaccaccagg
    11761 gctgcaaaac ttcacctcta tcaatcttga taataaccaa ctcacacatt ttgatgcaac
    11821 caactacgat agactcgtaa agctaagtct gaatagtaat gctcttgagt caataaattt
    11881 tcctcaaggc agaaatgtaa gtattacaca tatatctatg aataataatg ctctcagaaa
    11941 tattgatata gataggcttt catcagttac ttattttagt gcggcacata atcaactaga
    12001 gtttgtgcaa ttagaatctt gcgaatggct gcaatacctg aatctcagcc ataatcaatt
    12061 aactgatatt gttgcaggta ataaaaatga actcttactg ctggatctat cccataataa
    12121 actaacaagt ttacacaatg tcttatttcc caacttgaat acgttactta ttaacaacaa
    12181 tttgctttct gaaattaaaa tattctatag caacttccgc aatgttcaga cattaaacgc
    12241 tgctaacaac cagttgaaat atataaatct tgatttcctg acttatcttc catctatcaa
    12301 aagtttaaga ctggacaata ataaaataac ccacattgat actaataata catccgatat
    12361 tggaacttta ttccccataa taaaacagag caaaaactta aattttttaa atgtttctgg
    12421 gaagaacaat tgacctacta tgcagctcat gttatttaat ttgttttccc cagcacttaa
    12481 gcttaatact ggcctggcaa ttctttcgcc tggtgcattt gaagttcact ctgacggaat
    12541 agatgcggat aacgaattgt ttcactatac tattaaaaaa gcatataccc catataatat
    12601 acacacttac aagacagagg aagttgtaaa ccagaggaat ataaaagtta aaaacatgac
    12661 ct
//
LOCUS       DAETQL010000102        12648 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.102, whole genome shotgun sequence.
ACCESSION   DAETQL010000102 DAETQL010000000
VERSION     DAETQL010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.102"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            758..841
                     /locus_tag="K0W65_003348"
     CDS             758..841
                     /locus_tag="K0W65_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBI3096485.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="K0W65_003349"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="K0W65_003349"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBI3096486.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="K0W65_003350"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="K0W65_003350"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HBI3096487.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="K0W65_003351"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="K0W65_003351"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBI3096488.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="K0W65_003352"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="K0W65_003352"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBI3096489.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="K0W65_003353"
                     /pseudo
     CDS             complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="K0W65_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="K0W65_003354"
     CDS             complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="K0W65_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBI3096490.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8830..9165
                     /gene="uspB"
                     /locus_tag="K0W65_003355"
     CDS             8830..9165
                     /gene="uspB"
                     /locus_tag="K0W65_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBI3096491.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="K0W65_003356"
     CDS             complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="K0W65_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBI3096492.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10967..12166
                     /locus_tag="K0W65_003357"
     CDS             10967..12166
                     /locus_tag="K0W65_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBI3096493.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3112 a         3171 c         3359 g         3006 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     6421 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
     6481 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
     6541 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg
     6601 tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt
     6661 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
     6721 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
     6781 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
     6841 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
     6901 tatccagctt gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca
     6961 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
     7021 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
     7081 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
     7141 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
     7201 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
     7261 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
     7321 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
     7381 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
     7441 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
     7501 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
     7561 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
     7621 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
     7681 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
     7741 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
     7801 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
     7861 ttttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
     7921 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     7981 gaacacgtcg tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc
     8041 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg
     8101 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     8161 gcctaggtcg ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag
     8221 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     8281 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     8341 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     8401 gagatcgact gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     8461 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     8521 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     8581 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     8641 atattatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
     8701 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
     8761 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
     8821 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
     8881 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
     8941 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
     9001 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
     9061 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt
     9121 ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa
     9181 taacgtataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca
     9241 ggaactgcaa ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa
     9301 acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca
     9361 ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat
     9421 aactcgccag gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg
     9481 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
     9541 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga
     9601 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac
     9661 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
     9721 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
     9781 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
     9841 ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg
     9901 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
     9961 cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca
    10021 acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga
    10081 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    10141 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    10201 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    10261 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    10321 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    10381 atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    10441 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    10501 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    10561 ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    10621 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    10681 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    10741 gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca
    10801 aagtgaaata tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt
    10861 atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg
    10921 aggaatcttt acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga
    10981 tgccattatt ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt
    11041 aggacgccgg gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc
    11101 tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca
    11161 gaatccgcat ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct
    11221 ggtcaataaa cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga
    11281 ctccgcgcag cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt
    11341 cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct
    11401 gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc
    11461 ggggctgggc gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct
    11521 gccgacccgc gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca
    11581 ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg
    11641 tgagaactta ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag
    11701 ctactggcaa ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac
    11761 cttcctgaat gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt
    11821 tcatctaccg aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc
    11881 gctaaaacag ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg
    11941 cgtacaaccc aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga
    12001 caccaacgaa ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat
    12061 cggcgaagtg atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc
    12121 gagtgcatgg gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc
    12181 cgtcagccgt ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt
    12241 ctggtgttaa ctcgctgttt tgttagcgaa ttaacgctat tgccgttgtg gttattttat
    12301 agtgtattta ttctcagaga atatatatta ctgaaataaa aaacacaaag aataaccaaa
    12361 ggaaattatt tgaaacgtaa aaggtatata tttttaatct gtaaaattta aaatcacctc
    12421 ctctggattg aatcaacttg ctctatttaa gacgattgtt tttacatttt caccacctgt
    12481 atcaggtggt gtttttttat tggcggcaat gcctttatct tatttttatt acgttgtaaa
    12541 tatggctatt cctaaatgaa atttgatttg ctcagtttat ttgtgatgtc aggggagtgc
    12601 ttgccatgag agtcgtgata ttattattca tcatttttag gtaatgct
//
LOCUS       DAETQL010000103        12389 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.103, whole genome shotgun sequence.
ACCESSION   DAETQL010000103 DAETQL010000000
VERSION     DAETQL010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.103"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            327..1967
                     /gene="trg"
                     /locus_tag="K0W65_003358"
     CDS             327..1967
                     /gene="trg"
                     /locus_tag="K0W65_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBI3096494.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2005..2928)
                     /locus_tag="K0W65_003359"
     CDS             complement(2005..2928)
                     /locus_tag="K0W65_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3096495.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3145..4488
                     /locus_tag="K0W65_003360"
     CDS             3145..4488
                     /locus_tag="K0W65_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBI3096496.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4713..6368
                     /gene="opgD"
                     /locus_tag="K0W65_003361"
                     /gene_synonym="mdoD"
     CDS             4713..6368
                     /gene="opgD"
                     /locus_tag="K0W65_003361"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HBI3096497.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6508..6732
                     /locus_tag="K0W65_003362"
     CDS             6508..6732
                     /locus_tag="K0W65_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBI3096498.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6795..7331
                     /gene="rimL"
                     /locus_tag="K0W65_003363"
     CDS             6795..7331
                     /gene="rimL"
                     /locus_tag="K0W65_003363"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBI3096499.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7326..8306)
                     /locus_tag="K0W65_003364"
     CDS             complement(7326..8306)
                     /locus_tag="K0W65_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096500.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8430..9422
                     /gene="tehA"
                     /locus_tag="K0W65_003365"
     CDS             8430..9422
                     /gene="tehA"
                     /locus_tag="K0W65_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBI3096501.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9419..10012
                     /gene="tehB"
                     /locus_tag="K0W65_003366"
     CDS             9419..10012
                     /gene="tehB"
                     /locus_tag="K0W65_003366"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBI3096502.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10315..10971
                     /locus_tag="K0W65_003367"
     CDS             10315..10971
                     /locus_tag="K0W65_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HBI3096503.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="K0W65_003368"
     CDS             complement(10989..11495)
                     /gene="tnpA"
                     /locus_tag="K0W65_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HBI3096504.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRTYFSNVCAYFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPGQVENRALYPRPEGWGFT
                     AHRIKSAI"
     gene            11503..12389
                     /locus_tag="K0W65_003369"
                     /pseudo
     CDS             11503..12389
                     /locus_tag="K0W65_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3228 a         2903 c         3113 g         3145 t
ORIGIN      
        1 tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa
       61 agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca
      121 cgaaattgcg tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc
      181 gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga
      241 cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga
      301 gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga ttaggttttt
      361 tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc ttagttctat
      421 tcgcattaag ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg
      481 atgttactgc ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt
      541 cagcccggat caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa
      601 tgaagcgaaa tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg
      661 cttatcagaa tcgaccggtg aaaacacctg ctgatgaagc cttcgacact gaattaaatc
      721 aacgctttca ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca
      781 tgtttgaagc gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata
      841 ccgatatttt gaacaaagcc gttaagatac gtagcaccag agccaaccaa ctggcggaac
      901 tggcccatca gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc
      961 tggtcatgac gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac
     1021 atgccgcaca acggattgaa aaaatcgcca gtggcgatct gacgatgaat gatgaaccgg
     1081 cgggtcgtaa tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg
     1141 ggatgacagt agggactgtt cgacagggcg cggaagagat ttatcgtggc accagcgaaa
     1201 tttcagctgg caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac
     1261 aaactgccgc cagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc
     1321 atcatgccag caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg
     1381 tttccggtgt agtaaaaacg atgggcgcta tctccacgag ttcgaagaaa atttctgaga
     1441 tcaccgccgt catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg
     1501 ttgaagccgc gcgagcgggt gagcaagggc gtggatttgc cgttgtcgcc agcgaagtac
     1561 ggacactcgc aagccgcagc gctcaggcag cgaaagagat tgaaggcttg atcagtgaat
     1621 cagtcaggtt aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca
     1681 ctattgttga tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg
     1741 atgaacaaag tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga
     1801 cgcaacagaa tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac
     1861 aggctgcacg attaactgag gcggtgaacg tattccgtct gaacaaacat tctgtgtcgg
     1921 cagaacctcg cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga
     1981 gggatcgaca gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat
     2041 caactggcaa ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc
     2101 tgcaccatgt cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac
     2161 agcgccagaa gggacaaacc acacgtaatc gtattcaacg gtaagttgac gagatagcga
     2221 agtagacagc gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc
     2281 atctgaatgc tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac
     2341 ccggcttagc gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcaa
     2401 cgattcaaga aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg
     2461 accaatgccg atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat
     2521 tgtcgcaact tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg
     2581 taatatcccc agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagacc
     2641 ttctttacga tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg
     2701 cgttaaaaat tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa
     2761 gcgagcgccc gtcagctgct ccagttcatt caatgtatta gagagcgcag gttgactcaa
     2821 attaagggtt tcagctgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg
     2881 aaggtggcgc aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa
     2941 aaacgaagcg gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat
     3001 attattaaca attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg
     3061 cgagatcaca gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat
     3121 tactgaccac tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa
     3181 cagttttcgc aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg
     3241 ctggaactgg tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa
     3301 aaaatggtaa atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc
     3361 gttgggactg cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac
     3421 ccggtgagct attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg
     3481 cccattgatg atgcttctct ggcgcgtaat cccttccgcg tttttacatc cttactccgc
     3541 cttgagctta tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc
     3601 gatatcttca ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt
     3661 aacgaaacac aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag
     3721 ttagcaacgg tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct
     3781 gatgtggtct gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt
     3841 gaccgggtgc agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg
     3901 ccgccgcgcc gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag
     3961 acggtgttgt ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag
     4021 cagcgtggcg tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac
     4081 gctggaaccg ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact
     4141 tttcctgaca gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg
     4201 acgccttcgg gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta
     4261 attgaacgtg gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc
     4321 gcggcgggga tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat
     4381 gccagtcgcg aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt
     4441 taccaggaag cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc
     4501 cgcctttgca aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa
     4561 aaaaatgccg atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt
     4621 ctagagtgag cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc
     4681 attcacaaca ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt
     4741 caatggctat ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcag
     4801 cattcgcggc agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc
     4861 tacagtcaat ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac
     4921 ctgacacgct ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa
     4981 aatcgctctg gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa
     5041 tgggattccg tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg
     5101 aaattcactt tcgcccggag ttgttcaaat acaacgatgc aggtgttgat accaaacaat
     5161 tagaagggca aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg
     5221 cgcgccgtga tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat
     5281 atcaatacgg tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag
     5341 agttccccga ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta
     5401 ccgtttatgc gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt
     5461 gtgagaaaag tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta
     5521 aacagctggg catttcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga
     5581 tgtgcgatac aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca
     5641 acggcgagtg gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca
     5701 ccgacaacaa cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc
     5761 aggacattat gggctggtat aacaaacgcc caagtctgtg ggtggaaccg cgtaacaagt
     5821 ggggtaaggg caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca
     5881 ttgtctgctt ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt
     5941 atcgtctgta ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga
     6001 cgcgtaccgg catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa
     6061 aatgggcgcg tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa
     6121 aaggcattga gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct
     6181 atattgaacc catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca
     6241 ctgatccggt cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat
     6301 ggctgtatca gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga
     6361 tgagttaatc gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat
     6421 cccgctcgca tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac
     6481 cgtgtcagcc actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt
     6541 ctgaaaaacg aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat
     6601 gaaattgcca gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg
     6661 aaaaaacaaa agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc
     6721 aaagaggtgt aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat
     6781 tgactggaga taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg
     6841 ttgcggaaag tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac
     6901 agcagtcgct aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc
     6961 agggtaatgt gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag
     7021 atgaacttat cggcgttatc tcgtttaatc gtattgaacc actgaataaa actgctgaaa
     7081 tcggctactg gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcaga
     7141 cattgattca tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg
     7201 tggacaatcc gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc
     7261 tgaaacaggc tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta
     7321 tcgattcata ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact
     7381 tttggaccac ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt
     7441 aactccacat ggttttcaat tatcaccgca cctgtaatac ggctctcacc ttgtattaca
     7501 acgtgctcgt cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca
     7561 tgatgtttta acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa
     7621 tgaatcgtcg gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact
     7681 ttagcgcaat cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca
     7741 aaaacttcgg cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt
     7801 gcctggtgga caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt
     7861 tgatggtcgg gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg
     7921 tgaccaaata ttctacattg cacgtaaact aatgagtcat gaatggtgac actgtcactt
     7981 atatatgcac cctggctgat ttcactatta tcgatccaga cgttatccgt tgcgtaaacc
     8041 tctccccaca gcacgctggt gccggtgata cgcgtattac cggaaatcac cgttccgccg
     8101 aacgcaatag cattctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg
     8161 cgatcgatcc agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata
     8221 atctgccgta ataacacact ttttttagtg ccatcttcct gataactaaa ggctcgctgt
     8281 tcttcactta ggcgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta
     8341 gcaaattctg cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac
     8401 tttataaatt aaacaaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg
     8461 caggctactt tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca
     8521 gccaggtttg gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca
     8581 tctggggatt attgactagc gcatttatta cccgactcat acgctttccg catagcgtgc
     8641 tggcggaagt tcgccatcca gtgctgagca gttttgtgag tttgtttccg gcaacgacga
     8701 tgctggtggc gattggtttt gttccgtggt ttcgcccact ggcggtgtgc atgttcagtt
     8761 ttggtgtcgt ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat
     8821 ctcaccctga agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta
     8881 tcagcgcaat ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg
     8941 caggcgtttt ctcatggcta agccttgaac cggtgatctt gcagcgtctg cgcagttcgg
     9001 gagaattacc cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg
     9061 cctgtagtgc ctggctgagc gtcaacggcg gcgagggtga cacgctggcg aaaatgcttt
     9121 tcggttatgg actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc
     9181 agccatttaa tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg
     9241 gtttgcatct ggggagtggc agcgataatg gatttttcca tacgctggcg gtgccgctgt
     9301 ttatctttac caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc
     9361 agggaaaatt gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat
     9421 gatcattcgt gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga
     9481 agtactggaa gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa
     9541 tggtcgtaac agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa
     9601 tgccatgagt atcgccaacg tcgagcgcat taaatccatt gaaaatctgg ataatttaca
     9661 cacccgggtc gttgatctga ataacctcac atttgatgga cagtacgatt ttattctttc
     9721 gactgtggtg ctgatgttcc ttgaggctaa aaccatccca gggttgattg ccaatatgca
     9781 acgttgcact aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta
     9841 tccatgtacc gtcggcttca cgtttgcctt caaagaggga gaattacgtc gatattacga
     9901 aggctgggag agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa
     9961 cggtaatcgt attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa
    10021 gcacaaaacg cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag
    10081 catatttttt atcgaaggga tgtgaaatta atcacagtag tcgaagtttt ttagcagctt
    10141 aacttactga aatttaacta ctgatgattg acttagcccc tttttcggca ttgactatgt
    10201 cgtctgaaaa ggggctgaaa aatttatttt caccaacact ttttttgcca caacacgaag
    10261 cggcgctttt tgctataact tagaaagtaa tataatcatc tcaggaaact attcatgcgt
    10321 accacatcat ttgcgaaagt tgcagcttta tgcggcttgt tggctctgtc tggttgtgcc
    10381 tctaaaatca cccagccaga taaatataac aattactctg atttaaaaga aacaacctca
    10441 gcaacaggta aacctgtttt acgttgggta gacccgagtt ttgatcaaag caaatatgac
    10501 agcatcgtct ggaacccaat cacttattat ccggtaccaa aaccttctac ccaggtaggg
    10561 cagaaagttc tggataaaat tttgaactat accaacaccg aaatgaaaga agcgatagcg
    10621 cagcgtaaac cagtggttac caccgctggg ccgcgtagtc tgattttccg tggggccatt
    10681 accggtgtag ataccagcaa agaagggctg caattctatg aagtggttcc tgttgcatta
    10741 gtggttgcgg ggacgcaaat ggctacaggc caccgtacca tggatactcg cctctatttt
    10801 gaaggtgagc tgattgatgc agcgactaat aaaccggtta tcaaagtcgt tcgtcagggc
    10861 gaaggtaaag acctgaataa cgaaagtacg ccaatggctt tcgaaaatat taaacaagtt
    10921 attgatgaca tggcgaccga tgccaccatg tttgacgtta acaaaaagta gttccagacg
    10981 cgccatcgtt agatggcgct ttttatccgg tgcgccgtaa aaccccatcc ttcagggcgg
    11041 ggatataagg cgcggttttc cacctgacca ggtgtttgct gttgttcaat gtattggcgg
    11101 atgacggata ttggcgcacc tccgcagcta ctggcaaaat agccaggact ccacaaaacg
    11161 cctttgtagt aatacctgac tgcaatatct ggtcgatctc gtcgcagtaa cctacccgat
    11221 acgcctttga ggctgtttac cagactggat atcgccagtt tggggggata gttaattaac
    11281 agatggacgt gatctggttc gccatccatt tcaaccagtt cagcttcaaa ataagcacat
    11341 acatttgaaa agtaagtgcg tagtttttct gtcgcgtcat ggtcaaaaat ctggcgtcgg
    11401 tatctggtga caaagactag gtgaacatgc atcaggaaaa cacaatgcct tccacgccgg
    11461 atatcggttt cttttttcac agaccaaagt atcatttgac ctgtgaaacg attacaggca
    11521 tttaaattcc agttaagacc aggtggtcaa caggagtgtg aaatgaggcg cttcgcaggc
    11581 gcatgtcgtt tcgttttcaa tcgtgctctg gcacgtcaga atgagaatca tgaggccggt
    11641 aataaataca tcccttacgg gaaaatggct tcctggctgg ttgagtggaa aaatgccact
    11701 gaaacgcaat ggcttaaaga ttctccctca cagccattgc aacagtcact gaaagacctt
    11761 gagcgggctt acaaaaactt cttccggaag cgggctgctt ttccccgatt caaaaagcgg
    11821 tgacagaatg atgcattccg ctacccgcag ggcgttaagc tcgatcagga aaacagccgt
    11881 atttttctgc cgaaactggg ctggatgcgc taccggaata gccgtcaggt cacgggtgtt
    11941 gtgaaaaatg tcactgtcag ccagtcctgc ggtacgtggt acatcagtat tcagacagaa
    12001 agtgaagtat ccactccggc tcacccttca gcatcaatgg tcgggctgga tgctggcgtg
    12061 gctaaactcg ccacgctgtc agatggcaca gtctttgagc ctgtaaacag ttttcagaaa
    12121 aaccagaaga cgctggcgag acttcagcga cagttaagcc gcaaggtcaa attcagcaac
    12181 aactggcaga agcagaaacg caaaatacag cgactgcatt cctgtatcgc aaatatccgc
    12241 agggactacc ttcacaaagt cacaacggcc gtcagcaaaa accacgcaat gatagtcatt
    12301 gaggatttga aggtcagcaa catgtcaaag tcagcagcgg gtacggtcag ccagccgggg
    12361 cgcaatgtcc gggcaaaatc aggtttaaa
//
LOCUS       DAETQL010000104        12262 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.104, whole genome shotgun sequence.
ACCESSION   DAETQL010000104 DAETQL010000000
VERSION     DAETQL010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.104"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..98
                     /locus_tag="K0W65_003370"
     CDS             <1..98
                     /locus_tag="K0W65_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3096505.1"
                     /translation="FEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            95..496
                     /locus_tag="K0W65_003371"
     CDS             95..496
                     /locus_tag="K0W65_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3096506.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            507..1247
                     /locus_tag="K0W65_003372"
     CDS             507..1247
                     /locus_tag="K0W65_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HBI3096507.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1306..1692
                     /locus_tag="K0W65_003373"
     CDS             1306..1692
                     /locus_tag="K0W65_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBI3096508.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1701..2030
                     /locus_tag="K0W65_003374"
     CDS             1701..2030
                     /locus_tag="K0W65_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBI3096509.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            2002..5043
                     /locus_tag="K0W65_003375"
     CDS             2002..5043
                     /locus_tag="K0W65_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBI3096510.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5043..5372
                     /locus_tag="K0W65_003376"
     CDS             5043..5372
                     /locus_tag="K0W65_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3096511.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5372..6070
                     /locus_tag="K0W65_003377"
     CDS             5372..6070
                     /locus_tag="K0W65_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBI3096512.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            6075..6818
                     /locus_tag="K0W65_003378"
     CDS             6075..6818
                     /locus_tag="K0W65_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3096513.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6755..7357
                     /locus_tag="K0W65_003379"
     CDS             6755..7357
                     /locus_tag="K0W65_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBI3096514.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7418..10897
                     /locus_tag="K0W65_003380"
     CDS             7418..10897
                     /locus_tag="K0W65_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBI3096515.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10965..11564
                     /locus_tag="K0W65_003381"
     CDS             10965..11564
                     /locus_tag="K0W65_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBI3096516.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHRSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11628..>12262
                     /locus_tag="K0W65_003382"
     CDS             11628..>12262
                     /locus_tag="K0W65_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBI3096517.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLS"
BASE COUNT         2886 a         2913 c         3926 g         2537 t
ORIGIN      
        1 catttgaaga tgcccgcgac cgcatcattg ctgcggaaat gccgaaacag ctggggtatg
       61 ccctgaaaca acaactgagg ttacatctga gtaaatgaac cgacacacac aaatccgtca
      121 gagcgtgctg gcacgccttc gggaacagtg tggagacagc gccacgtttt ttgacgggct
      181 tccggcattt attgatgcgc aggaactgcc cgccgtggcg gtgtggctga gtgatgcgca
      241 gtacaccgga aaaatgacgg atgaggacga ctggcaggct gttctgcata ttgccgtttt
      301 cgtccgtgca caggcgccgg attctgagct ggatacgtgg atggagaaca ccatatttcc
      361 ggccatgaag gatattccgg tgctttccgg actcatcgac accatgatcc cactcggttt
      421 taactatcaa cgtgataatg agatggccac ctgggcgatg gcggaaatca cgtaccagat
      481 cacgtacacg aattaaggag gtggtaatga ccacaccaaa tccactggca aagacgaaag
      541 gtgcggggac gacgttctgg atgtataccg gcaacggcga tgcgtttgcg aaccctttgt
      601 cggacactga ctggctgcgt cttgcgatgg tgaaggatct gcaacctggc gaaatgaccg
      661 ctgatgcaga agatgacact tatctcgatg atgaagatgc agactggaaa acgacaaccc
      721 aggggcagaa atccgttggt gatacttcgg cgacgctggc ctggcgtccg ggtgacagcg
      781 ggcagaaaaa actggttcag ttgttcgact ccggtgaagt ctgcgcgttt cgtatcaaat
      841 atcccaacgg cactgttgat gttttccgcg gctggctgag ctcactgggt aaaaccattg
      901 cctcaaaaga cgtgatgacc cgcactgtga aaatcagcgg tgtggggcgt ccgtatctgg
      961 cagaggaagg cactgaaacc gtgggtgtta ccggactgac ggtggcaccg gcatctgtca
     1021 gtgtcaaagc gggagcaacc accacactga cctttacagt aaaacctgac ggggccagtg
     1081 acaaagcgat cagtgtgcat tcgtcagatc ctcagactgc ttcggtgacc ctgagcgggc
     1141 ttgtggccac ggtgaaaggc gtgaagcagg gcagtgtcag catcgtgggc atgacctctg
     1201 acggagagtt tgtggcagtg gctgcggtga ccgtcagcgc accataacag gacgatactc
     1261 atcattgccc cggttatccg gggctttttg catccggaga acatgatgtt tctgaaacag
     1321 gatacgttta attatgaaaa acagtccgtg gtgctcagtg agctgtccgg gctgcagaga
     1381 attgaatatc tgacgtttgt tcagcagcga acggcaaagt ttgatgcaca ggagggagaa
     1441 ctgccggagg ctgaacgaca gattgctttt ctgcgtatgg gaatggatat caatgcctgg
     1501 ctggtttccc gctcactgtg gaatgctgag cagtctcagg atgttgagac gctttgcgca
     1561 tccattatga caacatggtc gtatgatgcg ctgggcgcgg gggcggagag ggttctgtcg
     1621 ctgagcggta tggggaccat tgagaatgcc ggggatgatg atcatgaggc gctgacgccg
     1681 gaaaagtcct gacgcgggaa atgcagtttg tcatgcggct tgcccgggag ttccggcggg
     1741 cagactggcg gcggatgctg tcggatatgt cggccactga gcttggtgag tggggcgatt
     1801 atttccggat gcagagcttc agtgatgtgt ggatggatgc gcagtttgcc tcgctgaagg
     1861 cattgatcgt gagaatggtg tccggcagca gcgatgctgc ggtggctgat ttcagccttt
     1921 taccggaaga gaacgggata ccggagcgaa cggacgaaga actgatgcat cttggggaag
     1981 gtatttccgg aggtgtgcgt tatggaccag atagccaacc tggtcattga tttggggatt
     2041 gatgcggcag agtttaaaaa tgaaattccc cgtatcaaaa accttctgaa tggtgcagcc
     2101 agcgatgcag aacggtcttc tgcccgtatg cagcgtttta tggagcgtca gactcaggcg
     2161 gcccggcaga caatgcaggc ggcttcttcg gctgcaacag cagcatcagc ccatgcgcag
     2221 acggtggaga agaatgcacg ggctcatgaa cgcatggccc gtgaggtgga acaaacccgt
     2281 ctgcgcgtgg atgccctgaa tcagaaaatg cgcgaggaac aggcgcaggc cagggcactg
     2341 gcggaggcgc aggataaagc ggctgccgcc ttttatcgcc agattgacag tgtgaaacag
     2401 gccggtgcgg ggcttcagga attacagcgt attcagcagc agatccgaca ggccagaaac
     2461 agtggcgggg ttggtcagca ggattatctg gcgctgattt cggagatcac ggcgaaaacc
     2521 cgtgccctga cgcaggcaga ggaacaggcc acccggcaga aagcggcgtt tatccgccag
     2581 cttaaagagc aggcaacccg ccagaatctg tcgtcttctg agttgcttcg cgccagggcg
     2641 gctcagctgg gggtaagcag tgctgcagaa gtgtatatcc gcaaaatgga gcgggcagga
     2701 aaagccacac attcgctggg gctgaaaagt gcggcagccc ggcgggagct gggggtgtta
     2761 atcagtcaga tggcgcgagg caattttggt gcgctgaggg gatccgggat aacgctggct
     2821 aaccgtgccg gatggacagg cgcactgatg tcgccgaaag gcatgatgac tggcggcgtt
     2881 attggcggac ttgtcgcggc ggtcctgggt ctgggtaaag cctggcatga cggccggaag
     2941 gagggcgaga aatttaaccg tcagctggcg ctgacgggac attatgccgg tgtcactgtc
     3001 gggcagttgt ggaaactcag ccgggccata tccgggaatg gtatcacgca acatgcggca
     3061 gccggtgcgc tggcacaggt ggtgggcagt ggggcatttc atggaaacga tattggtatg
     3121 gtggcgaaag ctgccgcaca gatggagcga tcggttggcc agtcggtcag cgataccata
     3181 aatcagttta agcggctgaa ggatgatcct gtaaatgccg cgaaggcgct ggacaatgcg
     3241 ctgcattttt tgaatgccac tcagcttgag cagatacgcg tccttgggga gcaggggcgg
     3301 tccagtgatg cggcccggat cgccatgtct gcgctggcag aggaaaccgg taaacgcaca
     3361 tctgatattg ataataatct caatgcgctg ggtagtacgc tgcaaacctt gtctgactgg
     3421 tggaagcagt tctgggatgc ggccatgaat attggtcgtg aagactcgct ggatgcgcag
     3481 attgatgcgt tacaggaaaa aattcagcgc gcgaaaaaat atccgtggac aaacgcctcc
     3541 acacaggtgg agtacgatca gcagcgtctt aacgatcttc aggagaaaaa acgccggaag
     3601 gatttgcagg atgcaaaagc gcaggcagaa cggaattacc aggagcaaca gaaacgccgg
     3661 aatgctgaaa atgccgcgct gaaccggatg aatgaaacgg aagctgcacg acatcagcgg
     3721 gaaattgcgc gtattaatgc catgcagtac gccgaccagg ctgtcaggga tgcggcgata
     3781 cagcgtgaaa atgaacgtta cgaaaaagcc attaagaaaa atacacgggc aacccgtaat
     3841 gatgaggcca cccggttatt gctgcagtac agtcagcagc aggcacaggt ggaagggcag
     3901 attgctgctg ccagacagtc agcaggcatt gccactgaaa ggatgactga agcgcataaa
     3961 cagcttctgg ccctgcagca gcgcatcagc gacctggacg ggaaaaagct gacggcagat
     4021 gaaaagagtg tgctggcccg taagaatgag ctgattcagg cgctgacgct gctggatgtg
     4081 aaacagcagg agctgcagaa acagacagcg cttaacgacc tgagaaaaaa aacggttcag
     4141 ctgaccagcc agctggcaga caaagaacgt gcactgcgtg agcagcacaa tctggatatt
     4201 gccactgcag gtatggggga taagcagcgg cagcgctacc aggcacagtt gcgcatccgg
     4261 caggaatacc ggcaacagtt gcaacagctt gagaatgaca gtcgccagaa aggcacttac
     4321 gggacggagg actaccggag ggctgaggag gtgctgaagg ggagcctgaa gcgacaactg
     4381 aatgaaaaca aacgctactg gcaggaactg gaagtggcgc agggcgactg gaaaaacggt
     4441 gccatgcggg cgtttcagaa ttttacggcg gatgcggata atgcggcggg aacggcagaa
     4501 cagatgttta cagtggcatt cagcagtgcc ggtaatgcac tggcgacatt ctgtaccacc
     4561 ggtaagctga attttaaatc cttcacctct tccctgttgt cagatatggc cagaattatg
     4621 gcacagatgg ccatgatgca ggcggtaaag ggcgtcggtt ctttattcgg cttcacgact
     4681 aatgctgatg gcggtgttta ccagtctgct gatttgagtc gctacagtgg cacggtggtt
     4741 aaccgtccga cgttttttgc ttttgcaaaa ggcgcgggtg tgatggggga agcgggacct
     4801 gaagccattc tgccactgcg tcgcggtgct gatggtaagc tgggggttgt ggcggatact
     4861 ggtggttcag gcatggtgat gtttgccccg cagtacaaca ttgagatcaa taacgacggc
     4921 acgaacgggc agataggtcc ggctgccctg aaggtggttt atgacctcgg gaaaaaagcg
     4981 gccgcggact ttatgcaaca gcaggcccgt gatggtggcc ggttaagtgg agcataccgg
     5041 taatggagac gtttcactgg aaagtgcgcc cggatatgaa tgtggtatca gagccgaaag
     5101 tggtgacagt gaagctgggc gatggttatg aacagcgtcg tgcggcggga ctgaataacc
     5161 agttgtcgac ttacagcgtg acgatacgtg ttcgtaaagg tgaacaccca tctttaaaag
     5221 cctttctgga acggcacggt ggcgtccgcg catttcagtg gacgccacct tatgactgga
     5281 aactgatcag ggtggtttgt cgtaaatggt cggcaagcgt gggggcgtta tgggtgactg
     5341 taacggccga ttttgaacag gtggtgaact gatgcaggat atccggcagg aaacactgaa
     5401 tgaatgcacc cgtgcggagc agtcagcccg ggtggagctc tgggaaatcg atctgacaga
     5461 ggtcggtggt gagcgttatt ttttctgtaa tgagcagaac gaaaaaggtg agccggtcac
     5521 ctggcagggg cggcagtatc aggcataccc cattcagggg tcggggtttg aactgaacgg
     5581 caggggctgt gctgcccgtc cgacactgac ggtctctaac ctgcacggca tggtcaccgg
     5641 gatggcggaa gatctgcaga gtctggtcgg cggaacggtg gtccggcgta aggtttacgc
     5701 ccgttttctg gatgcggtga acttcgtcaa cggaaacagc gacgccgatc cggaacagga
     5761 ggtgatcagc cgctggcgca tcgagcagtg cagcgaactg agcgcggtca gtgcctcttt
     5821 tgtgctggcc acaccaacgg agacggatgg cgcggttttc ccggggcgta tcatgctggc
     5881 gaatacctgt atgtggactt accgttctga tgagtgtggt tacacgggca gggcagtggc
     5941 tgacgagttc gacaaaccaa caacggatat ccggaaggac aaatgcagca agtgtatgcg
     6001 cgggtgtgag ttgcgcaaca ataccggtaa tttcggcggt ttcctttcca tcaataaact
     6061 ttctcagtaa atccatgaca cagacagaat cagcgattct ggcgcacgcc cggcgatgtg
     6121 cgccagcgga gtcgtgtggc ttcgtggtga gaacgccgga gggagacagg tatcttccca
     6181 gcgagaatat ctccggtgag ccggaggaac ggttccggat ggctccggag gactggctgc
     6241 gggcacaaat gcagggtgag attgtggcac tggtccacag tcatcccggt ggtctgccct
     6301 ggctgagtga ggctgaccgg cggctgcagg tgcagagtga tttgccgtgg tggctggtct
     6361 gccgggggac gattcacaag ttccgctgtg tgccgcatct caccgggcgg cgctttgagc
     6421 acggggtgac ggactgttac acgctgttcc gggatgctta tcatctggcg gggattgaga
     6481 tgccggattt tcatcgcggg gatgactggt ggcgtcacgg tcagaatctc tatctggata
     6541 atctggaggc cacagggctg tatcaggtgc cgttgtcatc agcacaaccg ggcgatgtgc
     6601 tgctgtgctg ttttggttca tcggtgccga atcatgccgc catttactgc ggcgatggtg
     6661 agctgctgca ccatattcct gaacaactga gtaaacgaga gaggtatacc gacaaatggc
     6721 agcgacgcac acactccctc tggcgtcacc gggaatggca cgcatctgcc tttacgggga
     6781 tttgcaacga tttggccgcc gcatcgacct tcgtgtgaaa acgggggctg aagccatccg
     6841 cgcactggcc acacagctcc cggcgtttcg tcagaaactg agtgacggct ggtatcaggt
     6901 gcgcattgcc gggcgtgatg caggtgaaac cgaattgtct gcccgtctta atgagccgct
     6961 ggcaaatggt gcagtgatcc acatagtacc gcgtctggtg ggagctaaaa gtggcggtgt
     7021 gtttcaggcg gtgctggggg cagctgttat ggcggttgct atatggatgc cgggggtagg
     7081 aattatggcg agtaatctgc tgttttctct cggtgccagt atgacgcttg gcggtgttgc
     7141 acagatgctg gcaccgaaag ccagaactcc ccgtacacag acaacggata acggcaaaca
     7201 gaacacctgt ttctcctcac tggataacat ggttgcccag ggcaatgttc tgcctgttct
     7261 gtacggtgaa atgcgcgtgg ggtcgcgggt ggtatctcag gagatcagca cggcagacga
     7321 aggggatggt ggtcaggttg tggtgattgg tcgctgatgc aaaatgtttc atgtgaaacc
     7381 gcctgcgggc ggttttgtcg tttatggagc gtgaggaatg ggtaaaggca gcagtaaggg
     7441 gcataccccg cgcgaagcga aggacaacct gaaatccacg cagttgctga gtgtgattga
     7501 tgccatcagc gaagggccgg ttgacggtcc ggtggatgga ttaaaaagcg tgctgctgaa
     7561 cggtacgccg gtcctggata gcgaggggaa gacaaacttt tccggtgtta cggtggtgtt
     7621 ccgcgccggc gagcaggagc agacaccgcc ggaggggttt gaatcttccg gctcagagac
     7681 tgtgctgggt acggaagtga aatacgacac gccgatcacc cggaccatca cgtcggcaaa
     7741 cattgatcgt ctgcgcctga ccttcggtgt gcaggcactg gtggaaacca cctcaaaggg
     7801 ggaccggaat ccgtcggaag tccgcctgct ggttcagata cagcgtaacg gtggctgggt
     7861 gacggaaaaa gacatcacca ttaagggcaa aaccacttcg cagtatctgg cctcggtggt
     7921 ggtgggtaac ctgccgccgc gcccgtttaa tatccggatg cgcaggatga caccggacag
     7981 caccacagac cagctgcaga acaaaacgct ctggtcgtcg tacaccgaaa tcatcgatgt
     8041 gaaacagtgc tacccgaaca cggcgctggt cggcgtgcag gtggattcag agcagttcgg
     8101 taaccagcag gtgagtcgca attatcatct tcgcgggcgc attctgcagg tgccgtcgaa
     8161 ctataacccg cagacgcggc aatacagcgg tatctgggac ggaacgctta agccagcata
     8221 cagcaacaac atggcctggt gtctgtggga catgctcact catccgcgct acggcatggg
     8281 gaaacgtctt ggtgcggcag atgtggacaa atgggcgctg tatgtcatcg gccagtactg
     8341 cgaccagtca gtgccggacg gatttggcgg cacggagccg cgcatcacct gtaacgctta
     8401 cctgaccaca cagcgtaagg cgtgggatgt tctcagtgat ttctgctcgg cgatgcgctg
     8461 tatgccggta tggaacgggc agacgctgac gttcgtgcag gaccgaccgt cggataaagt
     8521 gtggacctat aaccgcagta atgtggtgat gccggatgat ggcgcgccgt tccgctacag
     8581 cttcagcgcc ctgaaggacc gccataatgt cgttgaggtg aactggattg atccggacaa
     8641 cggtcatgag acggcgacag agcttgtgga ggacacgcag gccattgtcc gttacggtcg
     8701 taacgtcacg aagatggatg cctttggctg taccagccgg ggacaggcgc accgcgccgg
     8761 gctgtggctg attaaaacgg aactgctgga aacgcagacc gtggacttca gcgtcggcgc
     8821 agaagggctt cgccatgtac cgggtgatgt cattgaaatc tgcgatgatg attatgccgg
     8881 tatcagcacc ggtggtcgcg tactggcggt gaacagccag acccggacgc tgacgctcga
     8941 ccgtgaaatc acgctgccgt cctccggtac cacgctgata agcctggttg acggaaatgg
     9001 caatccggtc agcgtggagg ttcagtccgt caccgacggc gtgaaggtga aagtgagccg
     9061 ggttcctgac ggcgttgcag aatacagcgt gtgggggctg aagctgccga cgctgcgcca
     9121 gcgcctgttc cgctgcgtga gtatccgtga gaacgacgac ggcacgtatg ccatcaccgc
     9181 cgtgcagcat gtgccggaaa aagaggccat cgtggataac ggggcacact ttgacggtga
     9241 ccggcgcggc acggtgaatg gtgtcacgcc gccagcggtg cagcatctga ccgccgaagt
     9301 caccgcagac agcggggaat atcaggtgct ggcgcgatgg gacacgccga aggtggtgaa
     9361 gggtgtgagc ttcctgcttc gcctgaccgt ggcagcggac gacggcagtg agcggctggt
     9421 cagcacagcc aggacgacgg aaaccacata ccgcttcagg cagctggcgc tggggaatta
     9481 cagtctgaca gtccgggcgg taaatgcccg ggggcagcag ggcgatccgg cgtcggtatc
     9541 gttccggatt gcggcaccgg cagcgcctgt cactattgaa ctgataccgg ggtattttca
     9601 gataacggtg gtcccgaaac tggctgtata tgacccgacg gtgcagtttg agttctggtt
     9661 ttcggaaaag cggattgcgg atatcaggca ggttgaaacc agcgcccgct atcttggcac
     9721 ggcgctgtac tggatagccg ccagtatcaa tatcaggccg ggccatgatt attattttta
     9781 cgttcgcagt gtgaacaccg ttggcaaatc ggcatttgtg gaggccgtcg gtcgggcgag
     9841 cgatgatgcg gaaggttatc tgagttttta taaagggttg atcaataaaa cgcatctcgg
     9901 caaggagttg tggacgcaga ttgataacgg tcagcttgcg ccggacctga ctgaaatcag
     9961 gacgtccatt acgaatgtca gcaatgaaat cacgcaaacc gtcaataaaa aactggaaaa
    10021 tcagagtgct gcaatccagc agatacagaa agttcaggtt gatacaaata ataacctgaa
    10081 cagcatgtgg gctgtgaagc tgcaacagat gaaggacgga cgcctttata ttgcgggtat
    10141 cggtgccggt attgagaata cgccagcagg aatgcagagt caggtgctgc tggcggcaga
    10201 caggattgcg atgattaatc ctgcgaatgg caacacaaag ccgatgtttg ttggtcaggg
    10261 cgatcagata ttcatgaacg acgtgttcct gaaacgcctg acggctccga ccattaccag
    10321 cggcggtaat cctccggcat tttccctgac acctggcgga cggctgacgg cgaaaaatgc
    10381 cgatatcagc ggtaacgtga acgcgaactc cgggacgctc aacaacgtca cgattaataa
    10441 gaactgtcgg gttctgggaa aattgtccgc gaaccagatt gaaggcgatc tcgttaaaac
    10501 agtgggcaaa cctttcccac gggactcccg ggcaccggag aggtggccat cagggaccat
    10561 taccgtcagg gtttatgacg atcagccgtt tgatcggcaa attgttattc ccgcggtggc
    10621 gtttcgcggt gctaaacatg agcggaagaa taacaatatt tattcgtcat gccgcctgat
    10681 agtgaagaaa aacggtgctg aaatttataa ccgaacgacc ctggataata cgctgatata
    10741 tacgggtgtt attgatatgc ctgccggtca cggtcacatg acgctggagt tttctgtatc
    10801 ggcatggctg gtaaatggct ggtatcccac agcaagtatc agcgatttgc tggttgttgt
    10861 gatgaagaaa tccacagcag gtatcagtat cagctgaatt ttataaccca gatacgggca
    10921 ccagaaatgg tgcctttttt attgcagaaa agcgagaggt aattatgcgt aaactttatg
    10981 ctgccatttt gtccgcagcc atctgtctgg ctgtatccgg tgcgcctgca tgggcgtctg
    11041 aacatcggtc cacgctgagc gcggggtatc ttcatgcctc gacgaacgct cccggcagtg
    11101 atgatctgaa cgggattaac gtgaaatacc gttatgagtt tacggacacg ctggggctgg
    11161 tgacgtcatt cagctatgcc aacgctgaag atgagcaaaa aacgcattac agtgataccc
    11221 gctggcatga ggattccgtg cgtaaccgct ggttcagcgt gatggcgggg ctgtctgtgc
    11281 gcgtgaatga atggttcagc gcgtatgcga tggcgggcgt ggcttacagc cgtgtgtcga
    11341 ccttctccgg ggattatctc cgcgtaactg acaacaaggg gaaaacgcac gatgtgctga
    11401 ccgggagtga tgacaaccgc cacagcaaca cgtctctggc gtggggggct ggcgtgcagt
    11461 ttaacccgac cgaatccgtg gccattgacc ttgcttatga aggttccggc agtggcgact
    11521 ggcgcactga cggtttcatc gtgggtgtcg gttataagtt ctgattagcc aggtaacaca
    11581 gtgttatgac agcccgctgg ttcaggcggg ctttttgtgg ggtgaatatg gcagtaaaga
    11641 tttcaggtgt actgaaagat ggggcgggta aacctgtcgt aaattgtgcg attgaactgc
    11701 gggccagaag aaccagtccg accgttgtgg cacacgttgt tgccacttgc gtgacggaca
    11761 ataacggtgc ttatgtgatt gaggctgagc cggggtatta cgaggttgcg cttcactgta
    11821 acggctggca gccaacccgt gtcggggata ttgatgtggc accgactgat gcaccgggga
    11881 cactgaacgc gtttctgaat gcaccaaagg atggtgattt acgtccggag gtgatgaagc
    11941 gctttgagga aatggtggcg caggcgcagc agagtgccgg ggctgcagcc ggaaacgcac
    12001 agcagacggc gcaggatgtg gcggcagccg caacggcccg tgatgatgca caacgttttg
    12061 cggagaaagc ccgacaggat gcaaccgtca cagctgagga cagaaaggcc actgcggaag
    12121 atgtgacaag cacaggagca aatgcagccg cagccggaca gagcgcacag gatgccgcag
    12181 gttatgcccg cgcagcagaa caggccaaaa atgacattga tgctgcgctg accggcactc
    12241 tgaaaatggc taaccatctg tc
//
LOCUS       DAETQL010000105        12240 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.105, whole genome shotgun sequence.
ACCESSION   DAETQL010000105 DAETQL010000000
VERSION     DAETQL010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.105"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..446
                     /locus_tag="K0W65_003383"
     CDS             <1..446
                     /locus_tag="K0W65_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBI3096518.1"
                     /translation="SACAPETGLQQLVATPVPDEQRISFWPQHFGLIPQWVTLEPRVF
                     GWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAG
                     IAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            462..938
                     /locus_tag="K0W65_003384"
     CDS             462..938
                     /locus_tag="K0W65_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HBI3096519.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            1001..1222
                     /locus_tag="K0W65_003385"
     CDS             1001..1222
                     /locus_tag="K0W65_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBI3096520.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            1385..1759
                     /locus_tag="K0W65_003386"
     CDS             1385..1759
                     /locus_tag="K0W65_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HBI3096521.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            1806..2180
                     /locus_tag="K0W65_003387"
     CDS             1806..2180
                     /locus_tag="K0W65_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBI3096522.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            2177..2668
                     /locus_tag="K0W65_003388"
     CDS             2177..2668
                     /locus_tag="K0W65_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096523.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            2680..2877
                     /locus_tag="K0W65_003389"
     CDS             2680..2877
                     /locus_tag="K0W65_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096524.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            2962..3522
                     /locus_tag="K0W65_003390"
                     /pseudo
     CDS             2962..3522
                     /locus_tag="K0W65_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            3594..4598
                     /locus_tag="K0W65_003391"
     CDS             3594..4598
                     /locus_tag="K0W65_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HBI3096525.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            4880..5161
                     /locus_tag="K0W65_003392"
                     /pseudo
     CDS             4880..5161
                     /locus_tag="K0W65_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(5311..5398)
                     /locus_tag="K0W65_003393"
     tRNA            complement(5311..5398)
                     /locus_tag="K0W65_003393"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5362..5364),aa:Ser,seq:gga)
     gene            5633..6571
                     /gene="ghrA"
                     /locus_tag="K0W65_003394"
                     /pseudo
     CDS             5633..6571
                     /gene="ghrA"
                     /locus_tag="K0W65_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6626..7363
                     /gene="ycdX"
                     /locus_tag="K0W65_003395"
     CDS             6626..7363
                     /gene="ycdX"
                     /locus_tag="K0W65_003395"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HBI3096526.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            7387..7941
                     /gene="ycdY"
                     /locus_tag="K0W65_003396"
     CDS             7387..7941
                     /gene="ycdY"
                     /locus_tag="K0W65_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HBI3096527.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            8043..8534
                     /locus_tag="K0W65_003397"
     CDS             8043..8534
                     /locus_tag="K0W65_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBI3096528.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(8667..9373)
                     /locus_tag="K0W65_003398"
                     /pseudo
     CDS             complement(8667..9373)
                     /locus_tag="K0W65_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(9400..9816)
                     /gene="csgF"
                     /locus_tag="K0W65_003399"
     CDS             complement(9400..9816)
                     /gene="csgF"
                     /locus_tag="K0W65_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HBI3096529.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(9841..10230)
                     /gene="csgE"
                     /locus_tag="K0W65_003400"
     CDS             complement(9841..10230)
                     /gene="csgE"
                     /locus_tag="K0W65_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBI3096530.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            complement(10235..10885)
                     /gene="csgD"
                     /locus_tag="K0W65_003401"
     CDS             complement(10235..10885)
                     /gene="csgD"
                     /locus_tag="K0W65_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBI3096531.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            11638..12093
                     /gene="csgB"
                     /locus_tag="K0W65_003402"
     CDS             11638..12093
                     /gene="csgB"
                     /locus_tag="K0W65_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBI3096532.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            12134..>12240
                     /locus_tag="K0W65_003403"
     CDS             12134..>12240
                     /locus_tag="K0W65_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HBI3096533.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGG"
BASE COUNT         3198 a         2890 c         2984 g         3168 t
ORIGIN      
        1 cttcggcctg tgcaccggag actggcctgc aacaactggt tgccactccc gtccctgatg
       61 aacagcgcat cagcttctgg ccgcagcatt ttggcctcat tccacagtgg gtcaccctgg
      121 aaccccgtgt cttcggctgg atggaccgtc tgtgtgaaga ctactgcggt ggtatctgga
      181 atctgtacac cctgaacaac ggcggggcat ttatggcacc cgaaccggat gacgatgatg
      241 acgaaacatg ggtactgttc aatgccatga acggtaaccg cgctgaaatg agcccggaag
      301 ccgccggcat tgctgcctgt ctgatgacgt acagtcatca tgcctgtcgt acggagtgtt
      361 atgccatgac ggtccattat taccggctgc gggattacgc cctgcagcat ccggaatgca
      421 gcgccattat gcgcatcatc gactgaatgg aggcagggac aatgcagcag ctttcctttc
      481 tgcccggaaa catgacgccc ggcgagcgca gtctcattct gcgggccctg caaaccctgg
      541 accgccatct tcatgaaccc ggcgtggcct tcacctccac ccgtgcggca cgggaatggc
      601 tgattctgaa catggcggga ctggagcggg aagagttccg ggtgctgtat ctgaacaacc
      661 agaatcagct gattgccggt gaaaccctct tcaccggcac catcaaccgt acggaagttc
      721 atccccggga agtgattaaa cgcgccctgt accacaatgc cgctgccgtg gtgctggcgc
      781 acaatcaccc gtccggtgaa gtcacaccca gtaaggcaga ccggcttatc accgaacgtc
      841 tggtacaggc actgggcctg gtggatatcc gggtgccgga ccatctgata gtcggtggca
      901 accaggtttt ctcctttgcc gaacatggtc tgctttaacc cgtcacaacc acatcacacc
      961 tgttttcact tttatcttct gtcttcagag gtatcacatc atgagaatca tcacccgtgg
     1021 tgaagccatg cgtattcacc aacagcatcc tgcatcccgt ctttttccgt tctgtaccgg
     1081 taagtatcgc tggcacggca gtgctgaagc gtatactggt cgtgaagtgc aggatattcc
     1141 cggcgttctt gccgtgtttg cagaacgccg taaggacagt tttggcccgt atgtccggct
     1201 gatgagtgtc accctgaact gatgttccgt cacccgggaa acgagtacga ccagatgacc
     1261 tcagacgccg ctgtggatga ggccattctc atcaatgcat acatcttcac cgaaaccggt
     1321 cagcgcgccg gctgacctgt catcgttaaa ccattcttaa ccacttttca gagaggattt
     1381 tatcgtgtca gacaaactct ccgggataac tcatcccgat gacaatcacg accgcccctg
     1441 gtgggggctt ccctgcacag tgaggccctg ttttggcgct cgtctggtgc aagagggtaa
     1501 ccgcctgtat taccttgccg accgcgccgg tatcagaggc cgcttcagcg acgcagatgc
     1561 ataccatctg gaccaggcct ttccgctgct gatgaaacaa ctggaactca tgctcaccag
     1621 cggtgaactg aatccccgat atcagcatac cgtcacgctg tatgcaaaag ggctgacctg
     1681 cgaagccgac accctcggct cctgtggtta cgtttatctg gctgtttatc cgacaccagc
     1741 acccgcaacc acctcataac cccgaagctc tcacctttag cactaccagt tacagagaac
     1801 ttaagatgaa aacattatcc gacacacatg tacgggaggt atctcgctgc ccgtctcccg
     1861 tcaccatctg gcagacactg ctcatccgac tgctggacca gcattatggc ctcacactga
     1921 atgacacacc gtttgtcgat gaacgtgtga ttgagcagca tattgaggca ggcatttcac
     1981 tgtgtgatgc agttaatttt ctcgtggaaa aatacgcgct ggtgcgtacc gaccagccgg
     2041 gattcagcac ctgtccccgc tctcagttaa taaacagtat tgatatcctc cgggctcgcc
     2101 gggcgaccgg cctgatgacc cgcgacaatt acagaacggt aaataacatt accctgggta
     2161 agtatccgga ggcgaaatga tgaaactggc cctgacactg gaagccgaca gcgttaacgt
     2221 acaggcactg aacatagggc gcattgtcgt tgacgtcgat ggtattgagc tcgctgaact
     2281 gattaacatg gtctgcgata acggctactc ccttcgtgtt gttgatgaat ctgaccagac
     2341 ctcagcagaa tgcacgccac catttgctac ccttaccggc atacgctgca gtaccgcaca
     2401 tatcacggaa acggacaacg cctggttgta ctcgctgtca caccagacca gtgacttcgg
     2461 tgaatcagaa tggattcatt ttacaggtag cggatatctg ttacgtaccg atgcgtggtc
     2521 atacccggtt ctgcggctta aacgcctggg gctgtcaaaa acgttccgtc gtctggtcgt
     2581 cacactcatc cggcgttatg gcgtcagtct cattcatctg gatgccagcg ctgaatgcct
     2641 gccgggttta cccactttcg actggtaacc aggaacaaca tgaaatcatt aaccacggaa
     2701 acagcgctgg atattctgat tgcgtggctg caggacaata tcgactgcgg atcgggaatt
     2761 atctttgaca acgatgagga taaaacggat tcagcagcac tgttgccctg tatcgaacag
     2821 gcccgggagg atgtccgtac cctgcgtcat ctgcagcttc tgcaccagaa ccggtgaagc
     2881 tcactcacca gacttcattc ctctgacgcc agcctgcaca gggctggcgt tttcatttat
     2941 ctgcaaaaag gaatatcgat tatgtcagat atcacaatct cccgtccgga agtggtcaac
     3001 gggcatacgg acgttatctg ctcaaccagc attcgccaca ttctggctgt acgaaagagt
     3061 acactgttgc aaatcgacac acttatccga caactggctg aaatctcggt gctgacggaa
     3121 agcatcggag gtaaaactgc cccggactgg gccatgaaac aggattttcg ctgcggctgc
     3181 tggctgatgg agaaacccga aacagcaatg aaagccatta cccggaatct tgatcgcgaa
     3241 atctggcgtg acctgatgca acgttccggg atgctgtcat taatggatac gcaggcccgt
     3301 gatacatggt accggtcact ggagtacgat aattttccgg aaatcagcga agcgaacatt
     3361 ctgagcacat ttgaacaact gcaccagaat aaggatgagg tgtttgagcg aggggtgatc
     3421 aacgtcttca gagggctgaa ctggaattac aaaaccaatt gcccctgtaa atttggcagt
     3481 aaaattatcg tcaacaatct ggtgagatgg gaccggtggg gataatgata tggtcacccc
     3541 caccttaaca gcagtacgct gttaaggtaa atccctttaa cggagagacc atcatgcaga
     3601 acatcacact tattggtatc gatctcggaa agcactcctt ccacattcat tgtcaagaca
     3661 agtctggtaa ggctcttctg cgtaaaaaat ttacccgtac taaactgatg cagtttctgg
     3721 cgacatgtcc gccctcagtt gttgtaatgg aagcctgtgc gggtgcacac tttatggctc
     3781 gctgtatctc tgattttggg catgaaacta agctgatttc tccacagttc gttcgcccgt
     3841 ttgtcaaaag taataagaat gattttgttg atgcagaagc catatgcgaa gcggcttcac
     3901 gaccatcaat gcgttttgtc cagccaagaa ctgagaccca gcaggccatg agggcgttac
     3961 accgggtcag agaatcgctc atcagagata aggtgaaaac aaccaaccag atccacggtt
     4021 tcttgctgga atttggaatc agcctgccga taggtgatgc tgtaataaaa cggttgtctc
     4081 tcgtgttggc tgaacacgaa atccccgaat atctgagccg ccttttggtg aggttgcata
     4141 cccactactt ttaccttgtt gagcaaatag cagagctgga atcagaatta agtcagtcaa
     4201 tcaacgctga tgataccgct cagcgcatta tgacaattcc tggtgttggt ccgattactg
     4261 ccagcttact atcgtcgcaa cttggtgacg ggaaacagtt ctcatgcagc agggattttg
     4321 cagcttccac aggacttgtt ccccggcagt atagcactgg aggaaaatcg actctgttgg
     4381 gtatcagtaa gcgaggtgat aaaaatttac gccggttact ggttcagtgt gcccgctcat
     4441 tcataatgcg acttgaacgc aagcatggaa aacttgctga gtgggttcgg gagcaactca
     4501 acaagaagca ttcgaatgtg gttacctgcg ctctggcaaa taaactggct cgaattgcct
     4561 gggcaatcac tactcagaaa aaagaatatc aggcctgata tagatgctga tataactcaa
     4621 ttagtttatt ttaaacaata agatacaagt tacctttctg gttttgcgaa gactgattat
     4681 tgatgacatg aacggcaaat cgacctgatt aaaaacctaa cagatgaaat ggctcactga
     4741 agccgagggg atttttagga ttatcaggtg cggaactcat cggggcgcgg gcatccttgc
     4801 ccacagagac gccggataga tttaagcaag ccaaccatta atcaaaaatc agtgttgcaa
     4861 aaacgggggt gaccatagat ttcatcttat caccggacaa caggctgacc gactcgccga
     4921 tctggaaaga atgttgcacc tgttcagcgg taaaccaatc cccgacaacc ggggaaacat
     4981 cacccttcgt ctggatgatc acattcagtc tgttcagggt aaagagagct atgaagatga
     5041 gatgttcagc atcagatact ttaagaaggg ctccgcgcat atcacgttca gaaaaccaga
     5101 actggttgac agactgaatg atattatcgc aaaacactat cctgatatgc tggcagtata
     5161 gctttgccag tatgctcaaa gatcggaaat tgtccgataa gatatgatcg cggggcatat
     5221 aaaccggttt cagatggcat acccgatggc atactgtctg gaaggaaaaa taaaaaagcg
     5281 catgttaaac aataacatgc acttataatc tggcggtgag ggggggattc gaacccccga
     5341 tacgttgccg tatacacact ttccaggcgt gctccttcag ccactcggac acctcaccaa
     5401 attgtcgttc ctgtcttgct ggaacgggcg ctaatttagg gaaatcatga cctgaggtca
     5461 acaaactttt tgaaaaaatc gcgcgtttat tcaaacattc aatcaatgtg tggttttaat
     5521 aagcgaaatc tgcttttttt gccaccgacc acggatttgt tatgctggtg gcctttgtag
     5581 atcataacga taagtgcgaa taaatttcgc acaacgcttt tcgggagtca gtatggatat
     5641 catcttttat cacccaacgt tcgataccca atggtggatt gaggcactgc gcaaagctat
     5701 tcctcaggca agagtcaggg catggaaaag cggagataat gactctgctg attatgtttt
     5761 agtctggcat cctcctgttg aaatgctggc agggcgcgat cttaaagcgg tgttcgcact
     5821 cggggccggt gttgattcta ttttgagcaa gctacaggca caccctgaaa tgctgaaccc
     5881 ttctgttcca ctttttcgcc tggaagatac cggtatgggc gagcaaatgc aggaatatgc
     5941 tgtcagtcag gtgctgcatt ggtttcgacg ttttgacgat tatcgcatcc agcaaaatag
     6001 ttcgcattgg caaccgctgc ctgaatatca tcgggaagat tttaccatcg gcattttggg
     6061 cgcaggcgta ctgggctgta aagttgctca gagtctgcaa acctagcgct ttccgctgcg
     6121 ttgctggagt cgaacccgta aatcgtggcc tggcgtgcaa agctttgccg gacgggaaga
     6181 actgtctgca tttctgagcc aatgtcgggt attgattaat ttgttaccga atacccctga
     6241 aaccgtcggc attattaatc aacaattact cgaaaaatta cctgatggcg cgtatctcct
     6301 caacctggcg cgtggtgttc atgttgtgga agatgacctg ctcgcggcgc tggatagcgg
     6361 gaaagttaaa ggcgcaatgc tggatgtttt taatcgtgaa cccttaccgc ctgaaagtcc
     6421 gctctggcaa catccacgcg tgacgataac accacatgtc gccgcgatta cccgtcccgc
     6481 tgaagctgtg gagtacattt ctcgcactat tgcccagctc gaaaaagggg agagggtctg
     6541 cgggcaagtc gaccgcgcac gtggctacta ataaagcatc aggattcctg ctatccttgg
     6601 cgggaattga atacaggaga gagttatgta tcccgtcgac cttcatatgc ataccgttgc
     6661 cagcacacat gcatatagca cattgagtga ttacattgcc caggccaaac aaaagggcat
     6721 taaacttttt gcgatcaccg atcatggccc ggatatggaa gatgcgccgc atcactggca
     6781 cttcattaac atgcgtatct ggccgcgagt ggttgatggg gtagggatcc tgcgcggcat
     6841 tgaagctaac attaaaaatg ttgatggtga aattgactgc agcggtaaaa tgtttgactc
     6901 gctggatctg attattgccg gttttcatga gccggttttt gcgccacatg acaaagcgac
     6961 caatacacaa gcgatgatct ccactatcgc cagcggcaat gtgcatataa taagccatcc
     7021 cggaaatccg aaatatgaaa tagatgttaa agccgttgct gaagcagccg cgaaacatca
     7081 ggtggcgctg gaaatcaata attcctcatt tttacactca cgtaagggca gtgaagacaa
     7141 ctgtcgtgcg gtagctgcag cggtacgtga tgctggaggt tgggtggcat taggctcgga
     7201 ttctcacacc gcgtttacca tgggggaatt tgaagagtgt cttaaaatcc tcgacgcggt
     7261 agattttccg ctagagcgca ttttgaatgt ttctccgcgc cgcttactga acttccttga
     7321 atctcgcggt atggcaccga ttgcggaatt tgcagacctt taattactag taatggaaat
     7381 atattaatga acgagttttc tatcctctgt cgtgtgctgg gttcgctcta ttaccgccaa
     7441 ccgcaagatc ctttactggt gccgctgttt actctgattc gtgaggggaa actggctgcg
     7501 aactggccac tggagcagga tgagttactg acgcgtttac agaaaagctg tgatatgacg
     7561 caagtctctg ccgattacaa tgcgttgttt atcggcgatg aatgtgccgt gccgccatat
     7621 cgtagcgcat gggttgagga cgcaacggaa gcggaagtgc gcgcttttct ttccgaacga
     7681 gggatgctac tggcggatac gccagccgat cacatcggca cattgctgct tgcggcttcc
     7741 tggctggaag atcagtcaac ggaagatgag agcgaagcac tggaaacact gttcagtgag
     7801 tatctgttac cctggtgtgg tgcgttcctc ggcaaagtgg aggcccatgc aaccacgcct
     7861 ttctggcgca ccatggcacc gctaacccgt gatgccatta gtgcaatgtg ggacgagctg
     7921 gaagaagatt ctgaagagta aatgtgatca tcgtcactca taactgcaac agataatttg
     7981 ttgttgcata aaatgtgtgc tcgatctcat tcatggccgc gttttctgct atcatgcgcg
     8041 gcatgaacat acttctctct attgcaatca caacaggcat tctctccggt atctggggat
     8101 gggtggctgt ttccctgggc ttactaagct gggcgggctt cctgggctgt acggcctact
     8161 ttgcttgccc gcaaggtggg ctgaaagggc tggcgatctc cgctgcaacc ctgcttagtg
     8221 gcgtggtgtg ggcgatggtc attatttacg gtagtgcact ggcaccacat ctggaaattc
     8281 tcggttatgt cataaccggt attgtcgcgt ttctgatgtg tattcaggcc aaacagctgc
     8341 tgctttcatt tgttccgggg acgtttatag gcgcatgcgc gacgtttgcc gggcagggtg
     8401 actggaaact ggtgttacct tcgctggcgc ttgggctggt atttggttac gcaatgaaaa
     8461 acagcggcct gtggctggcg gcgcgtagtg caaagacggc tcaccgtgag caggaaatca
     8521 aaaataaagc gtgaggggca ctcacgcttt cgcttaaaca gtaaatgccg gattattaat
     8581 tccggcattt ttatctgtca ggattccggt ggaaccgaca tatggcggta tttcaccaga
     8641 atgtcattct gccgttctgc tttattttac aaatcccaca gaccacggtc gataccatca
     8701 ttaatcagga aaatgacccc tgtttcgata gccgacatca gacacagcat aacgggttcg
     8761 tttgaggtgt aacccacttc cccttccagc aagcgctggt agtcaataaa gcggaaaacc
     8821 ccggcctgaa cttcatagga aagtatcgtc ttactggtgt tcaccgagga gaggatctca
     8881 ccggtactca cattgacgac acgcaggttc acggcaatct gatcgagctg gtattgcgtg
     8941 tctgcaccga tcccaaaata tcttgcccca acaccgcccg atttaacgtt gctttcataa
     9001 ccgataatcg aaccttcaac cataatattt gccgccgtta aagattgcag cgggattcgg
     9061 ttattaatgg caaccgtgcc gttttcttgt gccgcacgaa taaccttgcg ctcgttaagc
     9121 aggttttgta agccctggcg ctttgcggaa cagcagtgga gaagttactt gccgggtagg
     9181 gtttaaattg cccggtttcg tcctgaatgt tgtataccga aacaaagatc ttacccgtcg
     9241 gcgctggcag atgggtcaaa tctttgtagc tctgagcacg aggcattaat gtcggtctgg
     9301 cggcttcttt aggcggggcg gttaagcatc cgctcagtaa catgacggca accaaaagaa
     9361 ataagcgctg catgattatt ttccttatga agctggggct taaaaatcgg ttgagttatt
     9421 ttgtaaaccc gaaacctgga tggtcgaggt ttgtccggtt ttacgatctg tcacgttcaa
     9481 ctgcaattga ccatcgcggt tagcaatatc aacaatataa tcgttggtca ccatgcggcc
     9541 cggtttaccg gtattaatat tcgacagtag cccacctaaa atttgtgact ggatggcctg
     9601 agtaaagtta tctaacgctg agggtgtttc aataccaaag tcatcgttat agctcggatc
     9661 tttataagag ttttgggcct gagcgctatt taataaaaaa gcgccattat ttgggttacc
     9721 accaaagttt ggattacgga actggaaagt catggttcca gcccaactta atggcgaaat
     9781 aagcatgagt agaactactg catgtttgac acgcattgca gcctccgaac aattttttat
     9841 ttagaattca tcatgcgcca aatcgtccgt acttaataac gcctgattta tctggcgacg
     9901 atttagtgct tcttcagttt gaatcagtgc aaagacgaca gttttctcgg agtctctttt
     9961 caacgggaat aaaaaagtct ggaaaataac atcctgattg accgttattg tgatccagct
    10021 tccccatcgt gcactgggcc tttcattaat cgttaagtta cccgtatagt cactttccca
    10081 tttatcacta aaggctcggt aaaaatcatg gccaatagat gaaacagtat ggtcagttaa
    10141 caatcccggg acttctacct caacggcgtg aagattccct gcggcgaaca gaaattctgc
    10201 cgccacaatc cagcgtaaat aacgtttcat ggctttatcg cctgaggtta tcgtttgccc
    10261 aggaaaccgc ttgtgtccgg ttttttacgg ctatcttctt gaaaagatta taaagatgcg
    10321 ttttaaccgt attttcgctg atgaacaacg aacgagcgat ctcgttatta gacgcgccga
    10381 tacgcagctt attcaggatc tctttttccc gatgagtaag gagggctgat tccgtgctgt
    10441 tataacgata gttaccagaa tgtgtaatca ggtaactggc aagcttttgc gtaaagtagc
    10501 attcgccacg caggacgcct tgcaacccat tgacaacacg ttcttgatcc tccatggcat
    10561 aaaaaacgcc gttgatatga ggccagtttt caatgtcgcg gtacgggtaa tcttcaggcg
    10621 tatttagcaa caatattttg atattgttgt ttttcctgct caaagtatcc tgccaataat
    10681 ggataagctt tttatccgct tccatcatat ccagaagaat aatagagcct gaagagatat
    10741 cgtccagaga acgttgaata ttatgtaatt ttcctgtaat tgctagcgat tgtttaaggt
    10801 gctgcaagag agctgtcgcc tgcaaagaag gtttagtgat caacaataat gtatgaccat
    10861 gaatactatg gacttcatta aacatgatga aaccccactt ttttattgat cgcacacctg
    10921 acagctgcct ctaaaataga agcaccagaa gtactgacag atgttgcact gctgtgtgta
    10981 gtaataaatc agccctaaat gggtaaaata taaaactaat ggattacatc tgatttcaat
    11041 ctagccatta caaatcttaa atcaagtgtt aaacatgtaa caaaatgtaa ctcattatat
    11101 taaaatgtta acattaaggt tttattaagt ttagaaatga tagaaaagtt gtacatttgg
    11161 tttttattgc acaattttaa aaaatcatac aaatggtgct aacttactaa taatacatat
    11221 aaaaaatatt tcggtgtagt cctttcgtca tgtaaaacgt ttttgttttt tctccacacc
    11281 tccgtggaca attttttact gcaaaaagac gaggtttgtc acggcttgtg cgcaagacat
    11341 atcgcagcaa tcagcgacgg gcaagaagaa tgactgtctg gtgatttttg atagcggaaa
    11401 acggagattt aagagaaaac aaaatatttt ttgcgtagat aacagcgtat ttacgcgggt
    11461 tttaatactt tggtatgaac aaaaaaagaa aaatacaacg cgcgggtgag ttattaaaaa
    11521 tatttccgca gacatacttt ccatcgtaac gcagcgttaa caaaatacag gttgcgttaa
    11581 caaccaagtt gaaatgattt aatttcttaa atgtacgacc aggtccaggg tgacaacatg
    11641 aaaaacaaat tgttatttat gatgttaaca atactgggtg cgcctgggat tgcagccgca
    11701 gcaggttatg atttagctaa ttcagaatat aacttcgcgg taaatgaatt gagtaagtct
    11761 tcatttaatc aggcagccat aattggtcag gctgggacta ataatagtgc tcagttacgg
    11821 cagggaggct caaaactttt ggcggttgtt gcgcaagaag gtagtagcaa tcgggcaaag
    11881 attgaccaga caggagatta taaccttgca tatattgatc aggcgggcag tgccaacgat
    11941 gccagtattt cgcaaggtgc ttatggtaat actgcgatga ttatccagaa aggttctggt
    12001 aataaagcaa atattacaca gtatggtact caaaaaacgg cagttgtagt gcagagacag
    12061 tcgcaaatgg caattcgcgt gacacaacgt taattaccat tcgactttta aatcaatccg
    12121 atgggggttt tacatgaaac ttttaaaagt agcagcaatt gcagcaatcg tattctccgg
    12181 tagcgctctg gcaggtgttg ttcctcagta cggcggcggc ggtaaccacg gtggtggcgg
//
LOCUS       DAETQL010000106        12222 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.106, whole genome shotgun sequence.
ACCESSION   DAETQL010000106 DAETQL010000000
VERSION     DAETQL010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.106"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..493)
                     /locus_tag="K0W65_003404"
     CDS             complement(<1..493)
                     /locus_tag="K0W65_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3096534.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="K0W65_003405"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="K0W65_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBI3096535.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="K0W65_003406"
     CDS             complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="K0W65_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBI3096536.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2459..3325
                     /gene="folD"
                     /locus_tag="K0W65_003407"
     CDS             2459..3325
                     /gene="folD"
                     /locus_tag="K0W65_003407"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBI3096537.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3327..3539
                     /gene="ybcJ"
                     /locus_tag="K0W65_003408"
     CDS             3327..3539
                     /gene="ybcJ"
                     /locus_tag="K0W65_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBI3096538.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3647..4156
                     /locus_tag="K0W65_003409"
     CDS             3647..4156
                     /locus_tag="K0W65_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBI3096539.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="K0W65_003410"
     CDS             complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="K0W65_003410"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBI3096540.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5751..6245
                     /gene="ppiB"
                     /locus_tag="K0W65_003411"
     CDS             5751..6245
                     /gene="ppiB"
                     /locus_tag="K0W65_003411"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBI3096541.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6248..6970
                     /gene="lpxH"
                     /locus_tag="K0W65_003412"
     CDS             6248..6970
                     /gene="lpxH"
                     /locus_tag="K0W65_003412"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBI3096542.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7088..7597
                     /gene="purE"
                     /locus_tag="K0W65_003413"
     CDS             7088..7597
                     /gene="purE"
                     /locus_tag="K0W65_003413"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBI3096543.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7594..8661
                     /gene="purK"
                     /locus_tag="K0W65_003414"
     CDS             7594..8661
                     /gene="purK"
                     /locus_tag="K0W65_003414"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBI3096544.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8798..9691)
                     /locus_tag="K0W65_003415"
     CDS             complement(8798..9691)
                     /locus_tag="K0W65_003415"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBI3096545.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9688..10335)
                     /locus_tag="K0W65_003416"
                     /pseudo
     CDS             complement(9688..10335)
                     /locus_tag="K0W65_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10338..11570
                     /gene="allC"
                     /locus_tag="K0W65_003417"
     CDS             10338..11570
                     /gene="allC"
                     /locus_tag="K0W65_003417"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HBI3096546.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11581..>12222
                     /gene="allE"
                     /locus_tag="K0W65_003418"
     CDS             11581..>12222
                     /gene="allE"
                     /locus_tag="K0W65_003418"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HBI3096547.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYN"
BASE COUNT         2967 a         3159 c         3118 g         2978 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggagta
     1261 taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt taataaagcg
     1321 gcatgataca aagtgaacgt ggtttcgatt tacattgcca ccataccgct tcagacaacc
     1381 ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt
     1441 cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg
     1501 tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg
     1561 ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac
     1621 cgacacccgt tgctgttgtt gtatttgtac tggatacaat ggccaataaa ttatcattga
     1681 ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca ggatcgcagt
     1741 cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat
     1801 cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg
     1861 cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac
     1921 tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt aatttcatat
     1981 atattccttc aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa
     2041 gacactgata gtagtaaaat tcctttttat cctctaagaa tgtcttaatt gaaaatatgc
     2101 actctattct aaaaaataga gagccccgtt agatgaatac ttccgcgcaa aatatattca
     2161 ccacaaatat agacctgaag cggtaaatta ccaggctgaa aattcttttt atattgtcag
     2221 gtatttctta aattatctta atccttaaac aaggaaataa atcagttcca gatttacaac
     2281 gccatcatgt acgaaaaatg aagctttcaa tctcagcgac ctcagcgacg gtgcgcctaa
     2341 ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga aacctgacag cgcgccccgc
     2401 ttctgacaaa atagacgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat
     2461 ggcagcaaag attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca
     2521 aaaagttcag gctcgtattg cagccggact gcgggcacca ggactggccg ttgtgctggt
     2581 gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt
     2641 cgggttcgtc tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga
     2701 gcttatcgat gcgctgaatg ccgacaacac catcgatggc attctggttc aactgccgtt
     2761 accggcgggt attgataacg tcaaagtgct ggaacgtatt catccggaca aagacgtgga
     2821 cggtttccat ccttacaacg tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg
     2881 caccccgcgc ggtatcgtca cgctgcttga gcgttacaac attgatacct tcggcctcaa
     2941 cgccgtggtg attggcgcat cgaatatcgt cggtcgccca atgagcatgg aactgttgct
     3001 cgcaggttgc accaccaccg tgactcaccg cttcactaaa aatctgcgtc atcacgtaga
     3061 aaatgccgat ctgttgatcg ttgccgttgg caagccaggc tttattcccg gtgactggat
     3121 caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt
     3181 gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttcccgg
     3241 cggcgttggc ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga
     3301 atatcatgat ccacagggtg agtaacatgg cgacattttc tttaggtaaa catccgcacg
     3361 ttgagctgtg cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa
     3421 tcgcgattgc cgaaggccag gtgaaagtcg acggtgcggt tgaaacgcgc aaacgctgca
     3481 aaatcgtcgc cggtcagaca gtgagttttg caggacacag cgtacaggtt gttgcctgat
     3541 ccctttcccg gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc
     3601 cgttcatcca tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat
     3661 tacgcacgcc gccgttcccc tttgcattgg tttagggttg ggttcgaaag gtctgttatt
     3721 tgccggaatc atcctggcga tgctgcccga tgccgacgta ttgtcgttta aatttggcgt
     3781 tgcttacggc aatgtttttg gtcatcgcgg ttttacccat tcgctggtgt ttgcgtttgt
     3841 tgtcccgctc ttatgtgtgc tgattggacg tcgatggttc agggctgggc tgattcgctg
     3901 ctggctgttt ttaaccgtct cgttgctatc gcacagctta ctggattcgg taaccactgg
     3961 cggtaagggc gttggctggc tgtggccgtg gtcagatgaa cgctttttcg ctccctggca
     4021 ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acagcgtacg ggcatcaggt
     4081 gattatttcc gaattgatgt gggtgtggtt gccggggatg ctactgatgg gaatgttgtg
     4141 gtggcgcaga cgataaccgg atgcgaaaac tcgcatccgg caattgcgca attacttacg
     4201 acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt taagacggtc
     4261 acgtgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac gctgttgaat
     4321 taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt gcaggaacgc
     4381 ttccggttct tgctccagca ggcccaatac agcggaaagt ttacgcaggt gagatgccat
     4441 tgcattcgcc gctgccatat cttctgcttt cagacggttc acttcacgcg ccatatcaaa
     4501 cagcacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc
     4561 ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca gcgcagtgta
     4621 gaggcgctcc agcgccgcac gcgcctgctt caggttctct tcgctgtagt tcagctggct
     4681 gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt atttcagcac
     4741 atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt caaccatcac
     4801 cattccggag tgcatccagt agttcacata ctgaccatca tgggcacagg tggactgcgc
     4861 gatttcgttc tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga tatcaaagtg
     4921 gttacccagc tgcttgcagt tcatggccga acattcaatg tgccagccag gacgacccgc
     4981 gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca gaacgaagtc
     5041 catccggttg cgtttgtcat caaccacgtc aacgcgcgcg ccagcctgca gctggtcgag
     5101 atcctgacgc gacagcacgc cataagttgg atcggtcggg acgtcgaaca tcacgtcgct
     5161 gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt caataatttc
     5221 tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca aagcatcaaa
     5281 atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt cgccattttc
     5341 attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat acttcagttt
     5401 atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg
     5461 accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga cttccccggc
     5521 gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt ttagcatcga
     5581 agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata tttcccgcgc
     5641 gaatcagcat acattgcgtg atgatccgat cgtgcggtta tgctataaca ccaccctata
     5701 tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa atggttactt
     5761 tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca cctgaaacag
     5821 ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt ttccaccgtg
     5881 ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa caaaaagcca
     5941 ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt ggtacgctgg
     6001 caatggcacg tactcaggct ccgcactctg caactgcaca gttcttcatc aacgtggttg
     6061 ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg
     6121 ctgaagtggt tgacggcatg gacgtggtag acaaaatcaa aggtgttgca accggtcgta
     6181 acggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg accgttagcg
     6241 agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag aaccggcgat
     6301 caccgccggt tttctgcgtt ttttagcggg ggaagcccgc aaggccgacg cgctgtatat
     6361 tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac tccatcgcca
     6421 gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt tcattcatgg
     6481 caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga cattattgcc
     6541 ggaagaaaag gtgctcgaac tttatggtcg ccgggtgttg attatgcatg gcgacacgct
     6601 gtgcaccgat gacgcgggtt atcaggcttt tcgcgccaaa gtccacaaac cctggctgca
     6661 aatgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc gaatgcgcgc
     6721 gaacagcaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta accaaaacgc
     6781 tgtggtcagt gcgatggaaa aacatcaggt gcaatggcta atccacgggc atacccatcg
     6841 cccggcggtg catgaactta tcgccaacca gcaacctgct tttcgcgtgg tactgggtgc
     6901 ctggcatacg gaaggttcga tggtgaaagt cacggctgat gacgttgagc tgattcattt
     6961 tccgttttaa aaaacccaca actttgctga tttcacagcc acgcaaccgt tttccttgct
     7021 ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca caggagtttt
     7081 aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag
     7141 cgactgggct accatgcagt tcgccgtcga aatcttcgaa atcctgaatg tcccgcacca
     7201 cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg ccgaaagcgc
     7261 cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc acctgccagg
     7321 catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga gcgccgcact
     7381 gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc cggtgggtac
     7441 gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac aaattctcgc
     7501 gactcacgat aaagagctac accagcgtct gaatggctgg cgcaaagccc agaccgacga
     7561 agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac
     7621 gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc tgtctggccg
     7681 gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat taccgctgag
     7741 atcgaacgct ggccggaaac cgcattaacc cgcgagctgg cgcgtcatcc ggcctttgtg
     7801 aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaaacagct tttcgataag
     7861 ctccacctgc cgaccgcgcc gtggcagtta cttgccgatc gcagcgagtg gcctgcggtg
     7921 tttgatcgtt taggtgaact ggcgattgtt aagcgtcgca ctggtggcta tgacggtcgc
     7981 ggtcaatgga gtttacgcgc caatgaaacc gaacagttac cggcagagtg ttacggtgaa
     8041 tgtattgtcg agcagggcat taacttctct ggtgaagtat cgctggttgg cgcgcgcggc
     8101 tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga cggtattttg
     8161 cgcaccagcg tcgcttttcc gcaggccaac gcacagcagc aggcgcaagc cgaagagatg
     8221 ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga gtgttttgtc
     8281 accccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag cggtcactgg
     8341 acacaaaacg gtgccagcat cagccagttt gagctgcatc tgcgggcgat taccgatctg
     8401 ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat tggtagcgat
     8461 gtgaattatg actggctgaa attgccgctg gtgcatctgc actggtacga caaagaagtc
     8521 cgtccggggc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc gcgtctgact
     8581 gcgacgctgg aagccttaat cccgctgctg ccgccagagt atgccagcgg cgtgatttgg
     8641 gcgcagagta agttcagtta agcaggtgct catttgattg ccggatgcgg cgtaaacgcc
     8701 ttatccggct taccgctccg gtacccattg taggcctgat aagacgcgtc aagcgtcgca
     8761 tcaggcatgg tgcaccgatt gccggatgct ctcatcacta cagcgaaata caggtccccg
     8821 cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg ggtttactac
     8881 ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca tcggctttgg
     8941 caaatggcgc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc
     9001 agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca ttaatctgct
     9061 cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct gccccatctt
     9121 ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct ttgagcaaca
     9181 actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc acccggcgca
     9241 aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc tcttgttctt
     9301 ctggctgata aaccggacca ataaatttct ctggctgcaa aaacgccgga tcatcaggcg
     9361 aaacctcaat gcgcgtcagc accgtcgtaa caggcggcat ctgcggctgt gcgctcaagc
     9421 tctgcgccag catatagcca atcatcccct ggctttccgc caccagtaca tccagcggat
     9481 acggttctac ctctttccac gccagattct gtaacgccag cagccccacc tgcggcccgt
     9541 tgccgtgaac aatcgccagc cgataagaac gggccaggcg tgccagcgcg ggtacagcac
     9601 tggcgatatt gcgatattga ttttccgccg tcagcgcctc accgcgctgg agtaaggcgt
     9661 tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt gttggccaag
     9721 ccagaacccc agcagcgtat ctgcccctga cgtatgcccc agcgccagca gcgaatcaat
     9781 cgcaacagcg gtacgtttcg ggcaactcag agcatggaca aagtgcagga gtggcgaggc
     9841 gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgctgtgg taacgagttg
     9901 aagattgtcg gaacaggcaa aaaacgggcg accggcgcgc gcatctaaag cgccataata
     9961 ccaggccgcc agcagcattc cgctcaacgt gtcatcatga cttggcgtta atccggggcc
    10021 tttacccagc cagtgccgcc agtcggtctt aacgccattg agcgcggcct gaaaacagtg
    10081 acgaaactgg cgtaattcag cgggcagcgg atcgcttgcc gccatcgcca gtggcccgaa
    10141 aagtccggtt tcctccgcgc gttgcatcca tgcagctgca agcggttgag gatgcgaagg
    10201 cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac gcccgaggcg
    10261 aatcccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc ataacgcatc
    10321 gaattgcgtg cggcgaaatt acacattccc gtcaagctat agaagaaacg ctgccctggc
    10381 tttcctcttt tggcgctgac ccaacgggtg ggatgacccg tttactttat tcgccggaat
    10441 ggctggaaac ccagcagcaa tttaaaaaaa gaatggcagc aagtgggctg gaaacacgtt
    10501 tcgatgaagt ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtgg
    10561 ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg
    10621 gcgcgctggc ggcgtggctg gcaattgact ggttgaaaac gcaatacggc gcaccgttac
    10681 gtacggtcga agtggtggcg atggcagaag aagaaggcag ccgcttcccg tatgtcttct
    10741 ggggcagtaa aaatatcttt gggctggcga atcctgacga cgtgcggaat atctgtgatg
    10801 ccaaaggaaa tagttttgcc gatgcgatga aggcttgcgg atttactctg ccgaacgccc
    10861 cactaactcc gcgtcaggct ataaaagcct ttgttgaact gcatattgaa cagggctgtg
    10921 tgctggaaag taatgggcaa tcaattggcg tggtgaatgc aattgtcggg cagcgtcgct
    10981 atacggtgac gctgaacggc gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc
    11041 gtgatacggt ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga
    11101 tgggcgatcc gctggttctg acctttggca aagtagagcc gcgcccgaat acggtaaatg
    11161 tggtgccggg taaaaccacg ttcaccattg attgtcgtca taccgacgct gccgtgctgc
    11221 gcgatttcac ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggacattg
    11281 gtattgatat cgatttatgg atggacgaag aacccgtgcc gatgaataag gagctggtcg
    11341 ccaccctgac agaattgtgt gaaagagaaa aactgaatta ccgggtgatg cacagtggtg
    11401 ccgggcacga cgcgcaaatt ttcgcgcctc gcgtaccaac ctgcatgatt tttatcccca
    11461 gcatcaacgg gatcagccat aacccggcgg aacgcaccaa tattactgac cttgccgaag
    11521 gggtcaaaac gttggcactc atgctttatc aacttgcctg gcaggaataa ggagtcataa
    11581 atgggatatt taaataacgt caccggttac cgcgaagatt tactggctaa ccgtgcgatt
    11641 gttaaacacg gtaatttcgc actgttaacc ccggatggtc tgataaaaaa tattattccg
    11701 gactttgaaa attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt
    11761 gattatctgg tcacactgca tcaaaacggt ggcaaccaac agggcttcgg tggcgaaggc
    11821 attgaaacgt tcctgtatgt gatctctgga aatatcactg ccaaagccga aggcaaaaca
    11881 tttgccttaa gcgaaggtgg ctatctttat tgcccaccag gctccttaat gacgtttgtt
    11941 aacgcccagg ccgaagacag ccaaatcttt ttatataagc gccgctatgt tccggtagaa
    12001 ggccatgcac cgtggctgtt ttctggcaat gccagcgaac tggaacgcat tcactatgaa
    12061 ggtatggatg atgtgatcct gctggatttt ctgcccaaag agttaggttt tgatatgaac
    12121 atgcatatcc tctcttttgc accaggtgcc agccacggtt atatcgaaac acacgttcag
    12181 gaacatggtg cctatattct ttccggtcag ggggtttata ac
//
LOCUS       DAETQL010000107        12154 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.107, whole genome shotgun sequence.
ACCESSION   DAETQL010000107 DAETQL010000000
VERSION     DAETQL010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.107"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            128..997
                     /locus_tag="K0W65_003419"
     CDS             128..997
                     /locus_tag="K0W65_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HBI3096548.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1200..1553)
                     /locus_tag="K0W65_003420"
     CDS             complement(1200..1553)
                     /locus_tag="K0W65_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBI3096549.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1691..3337)
                     /gene="groL"
                     /locus_tag="K0W65_003421"
     CDS             complement(1691..3337)
                     /gene="groL"
                     /locus_tag="K0W65_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBI3096550.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3381..3674)
                     /locus_tag="K0W65_003422"
     CDS             complement(3381..3674)
                     /locus_tag="K0W65_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBI3096551.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3950..5206
                     /gene="yjeH"
                     /locus_tag="K0W65_003423"
     CDS             3950..5206
                     /gene="yjeH"
                     /locus_tag="K0W65_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBI3096552.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="K0W65_003424"
     CDS             complement(5222..5698)
                     /gene="fxsA"
                     /locus_tag="K0W65_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBI3096553.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6035..7471
                     /gene="aspA"
                     /locus_tag="K0W65_003425"
     CDS             6035..7471
                     /gene="aspA"
                     /locus_tag="K0W65_003425"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBI3096554.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7589..8890
                     /gene="dcuA"
                     /locus_tag="K0W65_003426"
     CDS             7589..8890
                     /gene="dcuA"
                     /locus_tag="K0W65_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HBI3096555.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9006..9344
                     /gene="cutA"
                     /locus_tag="K0W65_003427"
     CDS             9006..9344
                     /gene="cutA"
                     /locus_tag="K0W65_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBI3096556.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9320..11017
                     /gene="dsbD"
                     /locus_tag="K0W65_003428"
     CDS             9320..11017
                     /gene="dsbD"
                     /locus_tag="K0W65_003428"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBI3096557.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11054..11629
                     /locus_tag="K0W65_003429"
     CDS             11054..11629
                     /locus_tag="K0W65_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3096558.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11736..11811
                     /locus_tag="K0W65_003430"
     tRNA            11736..11811
                     /locus_tag="K0W65_003430"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11769..11771,aa:Phe,seq:gaa)
BASE COUNT         2931 a         3140 c         2980 g         3103 t
ORIGIN      
        1 ttttctgttt atatagagtg agcacggcta tttatgttat tttaacaact attactacca
       61 agcccattta tctggcttat aaattaaaat taattattta attgagaaat ggttagggag
      121 aacctacatg gccataagca tcaagggtgt taataccggt gttattcgca agagtaataa
      181 tttcattgca ctggcgctga aaattaaaga acctcgcaat aaagaatcgt tgttctttat
      241 gtctgtcatg gaactgcgcg acctgctgat tgcgctggaa agtcgtctgc atcaaaagca
      301 taaactggat gctgctgcac gccttcagta tgagcaagct cgcgataaag tcattaagaa
      361 aatggcagaa aacatcccag aaattctggt tgatgagctt aaaaatgccg atattaatcg
      421 tcgggttaac acgctggaac ttaccgataa tcaaggcgaa aatcttacct tcgtattaac
      481 gctacatgac ggtagcaaat gtgagttagt cgtcaatgag ttgcagattg aaatgctggc
      541 gcgagcaatc attcatgcaa ttaataatgc ggaaatgcgt gaactggcat tacgtattac
      601 ctcactgtta gatttcctgc cgttatacga cgttgactgc caggacaatg gcaatctgga
      661 atatgacacc tattcccaac cggaatggaa acataacttg ttcgaccatt atctggcggt
      721 gctttaccgc tttaaagatg aaagtggcaa agaacaattt agcggcgcag tggtgaaaac
      781 gcgtgaagca acgccgggta aagaaataga ggcgatcact cgccgaatgc ttgatttcag
      841 cccacgactg aaaaaactcg cgggtgtgcc atgtcaggtc tatgttcgca ccgtagcggc
      901 gaataacgcc caaccgctaa cccaggatca atgcttacgc gcgctgcatc acttacgtgt
      961 acaatccacc agcaaaactg caccacaggc gaaataatct catctttgct aatgcgaggc
     1021 tggagccatc cagcctgaaa tgactgataa cgtgatcgtt ggtcatgccg gatgcggcgt
     1081 aaacgcctta tccggcctac aaattctcaa gcattcaatc agttgcaatg cctgctgtag
     1141 gcctgataag cgaagcgcat caggcaataa tacgtttgtc acccagcctc tggatcacat
     1201 cagttcgggc acttataaac ctgaccgata atctggctat ccgtcgggac aaaactggac
     1261 aacattccct gcgacgggct gctgatgcca taaatcacgt taccgcccat tgcagccgcc
     1321 tggttgcgca gatcgtttgc tgcgccgcgc atagaaccgc cctcttctcc gtgttgcccg
     1381 gaaagccagt tgctttgctt acctgtcgca gtaccaatca gctggcactc tgcgcctggc
     1441 tgctcgtcca caatgcgtac actctgaccg gcagcactca attcgttgct ggagctacaa
     1501 cccgccatca gtagcgcggc accgacaatc cctgctaagt attttacgtg catgttattc
     1561 cccataatca atgagctgga cgccactcgc gtcatccgtg tctgaatctt atactagaaa
     1621 gatgaccaaa agaaaaaccc ccagacattt ctgcccgggg gtttgtttat ttctgcgaag
     1681 tgcagggcaa ttacatcatg ccgcccatgc cacccatgcc gcccataccg ccagcagcgc
     1741 ctaagtcagc tgcatcgttt ttcggcaggt cggtaaccat gcattcggtg gtgatcatca
     1801 ggccagccac agaagctgcg tactgcagag cagaacgggt tactttggtt gggtccagga
     1861 tacccatgtc gatcatgttg ccgtattctt cggttgctgc gttgtaaccg tagttgccgt
     1921 cgccgccttt aacggtgtta gcaacaacag acggttcttc gccgcagttc aggacgatct
     1981 gacgcagcgg agcttccatt gcacgcagtg caactttgat acccacgttc tggtcttcgt
     2041 tctgaccacg caggtcagcc agtttagacg ctacgcggat cagcgcaaca ccaccaccag
     2101 caaccacgcc ttcttctacc gcagcacggg tcgcgtgcag ggcatcttca acgcgtgctt
     2161 ttttctcttt catttcaact tcggtagcag cacctacttt gataactgca acgccgcctg
     2221 ccagtttcgc tacgcgctcc tgcagttttt cacggtcgta gtcagaagtt gcttcttcaa
     2281 tctgctgacg gatctgagca acacggccct ggattgcagc ttcttcaccc acgccatcga
     2341 tgatagtggt ggtgtctttg ttgatcacaa cacgtttagc ctgacccagg tcttccaggg
     2401 ttgctttttc cagctccata ccgatctctt cagagattac ggtaccgcca gtcagggttg
     2461 cgatatcctg cagcatagct ttacgacgat cgccgaagcc cggagcttta accgcagcaa
     2521 ctttcacgat gccacgcatg gtgttaacaa ccagagttgc tagcgcttcg ccttcaacat
     2581 cttcagcgat gatcagcagc ggtttgcctg ctttggcaac agcttccaga accggcagca
     2641 tttcgcggat gttggagatt ttcttgtcag ccagcaggat gaacgggctt tccagttcta
     2701 ctgcgccagt ttccggcttg ttgatgaagt aaggagacag gtagccacgg tcgaactgca
     2761 taccttcaac cacgtccagt tcgtcctgca gaccggtacc gtcttcaacg gtgataacgc
     2821 cttctttacc gactttgtcc atcgcttcag cgatcagttt acctacggtt tcgtcggagt
     2881 tagcggagat ggtaccaacc tgagcaatcg ctttagaatc agagcacggt acggacagcg
     2941 ctttcagttc ttcaactgca gcggtaaccg ctttgtcgat accacgtttc aggtccatcg
     3001 ggttcatgcc cgcagcaaca gctttcagac cttcagtgat gatagcctga gccagtacgg
     3061 ttgcagtggt ggtaccgtcg cctgcagcgt cgttcgcttt ggaggcaact tctttcacca
     3121 tctgcgcacc catattttcg aacttgtctt ccagttcgat ttcacgagca acggaaacac
     3181 catctttggt gatggtcggt gcaccgaaag atttatccag aactacgtta cggccttttg
     3241 gaccgagggt aactttcact gcatctgcca gtacgtttac gccgcgcagc attttcacac
     3301 gagcgtcgtt accgaatttt acgtctttag ctgccattat ctttattcct taaattcgta
     3361 tgttcagtgt cgtgcgcgga ttacgcttca acaattgcca gaatgtcgct ttcggacatg
     3421 atcaacactt cttcattgtc gatcttctcg gatttcacac cgtagccatc gttgaaaata
     3481 acgatgtcgc caactttcac gtccagcggc ttcacttcgc cattttcaag gatacggcca
     3541 ttgccgacag ccagcacttc gccgcgggtg gatttagccg ctgcagagcc ggtcagaacg
     3601 atgccgccag cagatttagt ttcaacttct ttacgcttga cgatcacgcg atcatgcaat
     3661 ggacgaatat tcattgataa ctctcctttg agaaagtccg tatctgttat gggtgacgcc
     3721 ggagcttacg tggtttcccg gctggtgacc agagaaatgg ggatgaggct tcgccccttc
     3781 aagggggaaa aagaaaaaaa attctgatca gcacaaaatc gggtgaaaac cctgattcac
     3841 ctcacatttc atcgcaattt cttcatcgcc gtcataagcg aatctgattg tgctaccatc
     3901 gaaaatctac gcagttgccc aaaatttggg cgcaatcgga tatcaaccca tgagtggact
     3961 caaacaagaa ctggggctgg cccagggcat tggcctgcta tcgacgtcat tattaggcac
     4021 tggcgtgttt gccgttcctg cgttagctgc actggtagca ggcaataaca gcctgtgggc
     4081 gtggcccgtt ttgattatct tagtgttccc gattgcgatt gtgtttgcga ttctgggtcg
     4141 ccactatccc agcgcaggcg gcgtcgcgca cttcgtcggt atggcgtttg gttcgcggct
     4201 tgagcgagtc accggctggc tgtttttatc ggtcattccc gtgggtttgc ctgccgcact
     4261 acaaattgcc gccggattcg gccaagcaat gtttggctgg catagctggc aactgttgtt
     4321 ggcagaactc ggtacgctgg cgttggtgtg gtatatcggt actctcggtg ccagttccag
     4381 tgctaatcta caaaccgtta ttgccggact tatcgtcgca ctgattgtcg ctatctggtg
     4441 ggcgggcgat atcaaacctg cgagtatccc cttccccgcg ccaggaaata tcgaacttac
     4501 cgggttattt gctgcgttat cagtgatgtt ctggtgtttt gtcggtctgg aagcatttgc
     4561 ccatcttgcc tcggaattta aaaatccaga gcgtgatttt cctcgtgctt tgatgattgg
     4621 tctgctgctg gcaggattag tctactgggg ctgtacggta gtcgtcttac acttcgacgc
     4681 ctatggtgaa caaatggcgg cggcagcatc gcttccaaaa attgtagtac agttgttcgg
     4741 tgtaggagcg ttatggattg cctgcgtaat tggctatctg gcctgctttg ccagtcttaa
     4801 catttatata cagagcttcg cccgcctggt ctggtcgcag gcgcaacata atcctgacca
     4861 ctacctggca cgcctctctt ctcgccatat cccgaataat gccctcaatg cggtgcttgg
     4921 ctgctgcgtg gtgagcacgt tggtaattca tgccttagag atcaatctgg acgctcttat
     4981 tatttatgcc aatggcatct ttattatgat ttatctgtta tgcatgctgg caggctgtaa
     5041 attattgcaa ggtcgttatc gactactggc ggtggttggc gggctgttat gcgttctgtt
     5101 actggcaatg gtcggctgga aaagtctcta tgcgctgatc atgctggtgg ggttatggct
     5161 gtttctgcca aaacgaaaaa cgccggaaaa tggcataacc acataatccg gcgtttcgac
     5221 attaatcctg gcgatcgtct ttatgatcaa ggcggtcgcg gtcatcatcc tttcgctgat
     5281 actcaccatc aaaagtatta ccgccaccgg tcccggcgct aaaaccgccg cctggcatgc
     5341 gagaaaagcg caaatgtggc atcaacttca ctgtcagatg cttttgcacc ggcggcaata
     5401 aaagcagaag accgaggaag tcggtgaaaa agcccggtaa taaaagcagc aaaccggcaa
     5461 taatcagcga aacactttta atcatctccg ccgcagggtt ttcacccgcc gccatttttt
     5521 gctgcatcag cacgaaattc ttaaagccct ggttacgtac cagtgacata ccgataactg
     5581 acgtgaatat aaccagcacg agggtcagca atacccccaa tacatgggca acctgaataa
     5641 agattgaaat ctcaatatag acataaagga aaatggcaat aaaaggtaac cagcgcaaag
     5701 gtttctcctg taatagcagc cggttaaacc cggctacctg aatgggttgc gaatcgcctt
     5761 cagcttatat tgtggtcatt agcaaaattt caagatgttt gcgcaactat ttttggtagt
     5821 aatcccaaag cggtgatcta tttcacaaat taataattaa ggggtaaaaa ccgacactta
     5881 aagtgatcca gattacggta gaaatcctca agcagcatat gatctcgggt attcggtcga
     5941 tgcaggggat aatcgtcggt cgaaaaacat tcgaaaccac atatattctg tgtgtttaaa
     6001 gcaaatcatt ggcagcttga aaaagaaggt tcacatgtca aacaacattc gtatcgaaga
     6061 agatctgttg ggtaccaggg aagttccagc tgatgcctac tatggtgttc acactctgag
     6121 agcgattgaa aacttctata tcagcaacaa caaaatcagt gatattcctg aatttgttcg
     6181 cggtatggta atggttaaaa aagccgcagc tatggcaaac aaagagctgc aaaccattcc
     6241 taaaagtgta gcgaatgcca tcattgccgc atgtgatgaa gtcctgaaca acggaaaatg
     6301 catggatcag ttcccggtag acgtctacca gggcggcgca ggtacttccg taaacatgaa
     6361 caccaacgaa gtgctggcca atatcggtct ggaactgatg ggtcaccaga aaggtgaata
     6421 tcagtacctg aacccgaacg accatgttaa caaatgtcag tccactaacg acgcctaccc
     6481 gaccggtttc cgtatcgcag tttactcttc cctgattaag ctggtagatg cgattaacca
     6541 actgcgtgaa ggctttgaac gtaaagctgt cgaattccag gacatcctga aaatgggtcg
     6601 tacccagctg caggacgcag taccgatgac cctcggtcag gaattccgcg ctttcagcat
     6661 cctgctgaaa gaagaagtga aaaacatcca acgtaccgct gaactgctgc tggaagttaa
     6721 ccttggcgca acagcaatcg gtactggtct gaacacgccg aaagagtact ctccgctggc
     6781 agtgaaaaaa ctggctgaag tcactggctt cccatgcgta ccggctgaag acctgatcga
     6841 agcgacctct gactgcggcg cttatgttat ggttcacggc gcgctgaaac gcctggctgt
     6901 gaagatgtcc aaaatctgta acgacctgcg cttgctctct tctggtccac gtgccggcct
     6961 gaacgagatc aacctgccgg aactgcaggc gggctcttcc atcatgccag ctaaagtaaa
     7021 cccggttgtt ccggaagtgg ttaaccaggt atgcttcaaa gtcatcggta acgacaccac
     7081 tgttaccatg gcagcagaag caggtcagct gcagttgaac gttatggaac cggtcattgg
     7141 ccaggccatg ttcgaatccg ttcacattct gaccaacgct tgctacaacc tgctggaaaa
     7201 atgcattaac ggcatcactg ctaacaaaga agtgtgcgaa ggttacgttt acaactctat
     7261 cggtatcgtt acttacctga acccgttcat cggtcaccac aacggtgaca tcgtgggtaa
     7321 aatctgtgcc gaaaccggta agagtgtacg tgaagtcgtt ctggaacgcg gtctgttgac
     7381 tgaagcggaa cttgacgata ttttctccgt acagaatctg atgcacccgg cttacaaagc
     7441 aaaacgctat actgatgaaa gcgaacagta atcgtacagg gtagtacaaa taaaaaaggc
     7501 acgtcagatg acgtgccttt tttcttgtga gcagtaactt aaaaataaca atctaatatc
     7561 aacttgttaa aaaacaagga aggctaatat gctagttgta gagctcatca tagttttgct
     7621 ggcgatcttc ttgggcgcca gattgggggg aataggtatt ggttttgcag gcggattggg
     7681 ggtgctggtt cttgccgcta ttggcgttaa acccggtaac atcccgttcg atgtcatttc
     7741 cattatcatg gcggttatcg ccgctatttc tgccatgcag gttgctggcg gtctggacta
     7801 tctggttcat cagacagaaa agctgctgcg ccgtaacccg aaatacatca cgatcctcgc
     7861 accgatcgtg acctatttcc tgactatctt tgctggtact ggcaacatct ctctggcgac
     7921 actgccagtt atcgctgaag ttgcgaagga acaaggcgtt aaaccttgcc gtccgctctc
     7981 tactgcagtg gtatccgcgc agattgcgat caccgcatcg ccaatctcag cggcagtggt
     8041 ttacatgtct tccgtgatgg aaggtcatgg catcagctac ctccatctgc tctccgtggt
     8101 catcccgtcc accctgctgg cggttctggt gatgtccttc ctggtcacta tgctgttcaa
     8161 ctccaaactc tctgacgatc cgatttatcg caagcgtctg gaagagggct tggttgaact
     8221 gcgcggtgaa aagcagattg aaatcaaatc cggtgcaaaa acgtccgtct ggctgttcct
     8281 gctgggcgta gttggcgtgg ttatctatgc aatcatcaac agcccaagca tgggtctggt
     8341 tgaaaaaccg ctgatgaaca ccaccaacgc aatcctgatc atcatgctca gcgttgcaac
     8401 tctgaccacc gttatctgta aagtcgatac cgacaacatc ctcaactcca gcaccttcaa
     8461 agcaggtatg agcgcctgta tttgtatcct gggtgttgcg tggctgggcg atactttcgt
     8521 ttccaacaac atcgactgga tcaaagatac cgctggtgaa gtgattcagg gtcatccgtg
     8581 gctgctggcc gtcatcttct tctttgcttc tgctctgctg tactctcagg ctgcaaccgc
     8641 aaaagcactg atgccgatgg ctctggcact gaacgtttca ccgctgaccg ctgttgcttc
     8701 tttcgctgcg gtgtctggtc tgttcattct gccgacctac ccgacgctgg ttgctgcggt
     8761 acagatggat gacacgggta ctacccgtat cggtaaattc gtcttcaacc atccgttctt
     8821 catcccgggt actctgggtg ttgccctggc cgtttgcttc ggcttcgtgc tgggtagctt
     8881 catgctgtaa tgatcaatcg cggggcgttc acgccccgct ttctttcccg ccgactaaca
     8941 tccttccccc gtccgttgta tagtgacctc tctcttgcgg ttccatctgt tcttgcgagg
     9001 tgtttatgct tgatgaaaaa agttcgaata ccacgtctgt cgtggtgcta tgtaccgcac
     9061 cggatgaagc gacagcccag gatttagccg ccaaagtgct ggcggaaaaa ctggcggcct
     9121 gcgcgacctt gatccccggc gctacctctc tctattactg ggaaggtaag ctggagcaag
     9181 aatacgaagt gcagatgatt ttaaaaacta ccgtatctca ccagcaggca ctgctggaat
     9241 gcctgaagtc tcatcatcca tatcaaaccc cggaacttct ggttttacct gttacacacg
     9301 gagacacaga ttacctctca tggctcaacg catctttacg ctgattctgc tactttgcag
     9361 cacttccgtt tttgccggat tattcgacgc gccgggacgt tcacaatttg tccccgcgga
     9421 tcaagccttt gcttttgatt ttcagcaaaa ccaacatgac cttaatctga cctggcagat
     9481 caaagacggt tactacctct accgtaaaca gatccgcatt acgccggaac acgcgaaaat
     9541 tgccgacgag cagctgccgc aaggcgtctg gcatgaagat gagttttacg gcaaaagcga
     9601 gatttaccgc gatcggctga cgcttcccgt aaccatcaac caggcgagtg cgggagcgac
     9661 gttaactgtc acctaccagg gctgtgctga tgccggtttc tgttatccgc cagaaaccaa
     9721 aaccgttccg ttaagcgaag tggtcgccaa caacgcagcg tcacagcctg tgtctgtttc
     9781 gcagcaagag cagcacaccg cgcaattgcc cttttccgcg ctctgggcgt tgttgatcgg
     9841 tattggtatc gcctttacgc catgcgtgct gccaatgtac ccactgattt ctggcatcgt
     9901 gctgggtggt aaacagcggc tctccactgc cagagcattg ttgctgacct ttatttatgt
     9961 gcaggggatg gcactgactt acacggcgct gggtctggta gttgccgccg cagggttaca
    10021 gttccaggcg gcgctacagc acccatacgt gctcattggc ctcgccatcg tctttacctt
    10081 gctggcgatg tcaatgtttg gcttgtttac tctacaactc ccctcttcgc tgcaaacacg
    10141 tctcacgttg atgagcaatc gccaacaggg cggctcacct agcggtgtgt ttgttatggg
    10201 ggcgattgcc ggactgatct gttcaccatg caccaccgca ccgcttagcg cgattctgct
    10261 gtatatcgcc caaagcggga acatgtggcc gggcggcggc acgctttatc tctatgcgtt
    10321 gggcatgggc ctgccgctga tgctaattac cgtctttggt aaccgcttgc tgccgaaaag
    10381 cggcccgtgg atggaacaag tcaaaaccgc gtttggtttt gtgatcctcg cactgccggt
    10441 cttcctgctg gagcgagtga ttggtgatgt atggggatta cgcttgtggt cggcgctggg
    10501 tgtcgcattc tttggctggg cctttatcac cagcctacag gctaaacgcg gctggatgcg
    10561 cgtggtgcaa ataatcctgc tggcagcggc attggttagc gtgcgcccac ttcaggattg
    10621 ggcatttggt gcgacgcata ccgcgcaaac tcaaacgcat ctcaacttta cacaaatcaa
    10681 aacggtagat gagttaaatc aggcgctcgt tgaagccaaa ggcaaaccgg tgatgttaga
    10741 tctttatgcc gactggtgcg tcgcctgtaa agagtttgag aaatacacct tcagcgaccc
    10801 gcaggtgcaa aaagcgttag cagacacggt cttacttcag gccaacgtca cggccaacga
    10861 cgcacaagat gtggcgctgt taaagcatct taatgtcctt ggcctaccga caattctctt
    10921 ttttgacgga caaggccagg agcatccaca agcacgcgtc acgggcttta tggatgctga
    10981 aaccttcagc gcacatttgc gcgatcgcca accgtgaacg acacttgcag tgggaaagac
    11041 ggaggaaaat agcgtgcaac gtgaagatgt actgggagaa gccctgaaat tattagaatt
    11101 acaagggatt gccaacacca cgctggagat ggttgctgaa cgtgtggatt atccactgga
    11161 cgagctacgc cgcttctggc cagacaaaga ggcaatcctc tacgatgcgc tgcgttatct
    11221 tagccaacag atagatgtct ggcgtcgtca gctgatgctg gacgaaacgc aaaccgccga
    11281 acaaaagctg ctggcacgtt atcaggcgtt atcggagtgc gttaaaaaca accgctatcc
    11341 gggctgtcta tttatcgctg cctgtacgtt ttatcccgat cctggccacc ctattcatca
    11401 actggccgat cagcaaaaaa gcgctgccta cgatttcacc cacgaactgt taaccacgct
    11461 ggaagttgac gatccggcga tggtagcaaa gcagatggaa ctggtgctgg aaggctgttt
    11521 aagccgaatg ctggtgaatc gtagccaggc ggatgtcgac actgcacatc ggctggcgga
    11581 agatatcctg cgcttcgccc gctgccgcca gggtggtgca ttgacctgac agaaacacag
    11641 aaaagaagcg atttgccgca atcttaagca gttgaatcgc ttttactgaa attaggttga
    11701 cgagatgtgc agattacggt ttaatgcgcc ccgttgcccg gatagctcag tcggtagagc
    11761 aggggattga aaatccccgt gtccttggtt cgattccgag tccgggcacc aaattcatat
    11821 caacggacct ccacggaggt ccgtttttcg tttcaggacg ccacgattta agcgttctgc
    11881 ctccaaatca attctaccga actcaaccag attctcccca catcaccagc aatttgcgag
    11941 catatcccaa tttgggaaaa tttgttcctg agctatagca ctaactgacg tgaaatgtcg
    12001 tgcggccccg tgatgctgtt gaacgtcaaa tgacgtcatc aggagcgtaa cgtatccata
    12061 aagcacaaca ccggtctgaa cgccgactga tgagattttc tgaatgagaa caaagagaaa
    12121 tgtgtcagtc cgcttgctca tgcaaagact aaca
//
LOCUS       DAETQL010000108        12114 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.108, whole genome shotgun sequence.
ACCESSION   DAETQL010000108 DAETQL010000000
VERSION     DAETQL010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.108"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..371
                     /locus_tag="K0W65_003431"
     CDS             <1..371
                     /locus_tag="K0W65_003431"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096559.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="K0W65_003432"
     CDS             complement(459..1103)
                     /gene="cheZ"
                     /locus_tag="K0W65_003432"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HBI3096560.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="K0W65_003433"
     CDS             complement(1114..1503)
                     /gene="cheY"
                     /locus_tag="K0W65_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HBI3096561.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="K0W65_003434"
     CDS             complement(1518..2567)
                     /gene="cheB"
                     /locus_tag="K0W65_003434"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HBI3096562.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="K0W65_003435"
     CDS             complement(2570..3430)
                     /gene="cheR"
                     /locus_tag="K0W65_003435"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBI3096563.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3449..5050)
                     /gene="tap"
                     /locus_tag="K0W65_003436"
     CDS             complement(3449..5050)
                     /gene="tap"
                     /locus_tag="K0W65_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HBI3096564.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSVLWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5096..6757)
                     /gene="tar"
                     /locus_tag="K0W65_003437"
     CDS             complement(5096..6757)
                     /gene="tar"
                     /locus_tag="K0W65_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HBI3096565.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="K0W65_003438"
     CDS             complement(6902..7405)
                     /gene="cheW"
                     /locus_tag="K0W65_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBI3096566.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="K0W65_003439"
     CDS             complement(7426..9390)
                     /gene="cheA"
                     /locus_tag="K0W65_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBI3096567.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9395..10321)
                     /gene="motB"
                     /locus_tag="K0W65_003440"
     CDS             complement(9395..10321)
                     /gene="motB"
                     /locus_tag="K0W65_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HBI3096568.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10318..11205)
                     /gene="motA"
                     /locus_tag="K0W65_003441"
     CDS             complement(10318..11205)
                     /gene="motA"
                     /locus_tag="K0W65_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBI3096569.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="K0W65_003442"
     CDS             complement(11332..11910)
                     /gene="flhC"
                     /locus_tag="K0W65_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HBI3096570.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11913..>12114)
                     /locus_tag="K0W65_003443"
     CDS             complement(11913..>12114)
                     /locus_tag="K0W65_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HBI3096571.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2785 a         3388 c         2933 g         3008 t
ORIGIN      
        1 acactacgct aatgagttgg gatgccgtgc tctatgatct acgtagtgaa aaaattatca
       61 gtaaagataa ttacttgacc gacttaaacc gcagacatct tgaacttgtt cttcatacta
      121 accccaatcc aagcggttcg gtaacgaaga ttttacgcac tatttttact aagcaggtaa
      181 agagtttagg gccggaactc tgtcatttct tgcaccatga actgacgaaa aaacgttatg
      241 aagtatggca tcactacgat gtactgcact accagtcatc gtcattttct cgttcacaat
      301 ttaatgcatt gataaattta ctggcgcaac aatcacgctt cgctgatcta aaattatcaa
      361 actacaaata accatcctcc acgtactccc tgaacgatac ccgccaccct gctatcggct
      421 tggcatcatc gcgcaggccg aagttcaggc aatacaaatc aaaatccaag actatccaac
      481 aaatcgtcca cctgatcctg actggctacc acgccggctt tgctggtatc gacctgaggt
      541 ccattaagca aactctggtt ttcacgtttt ggacgcgact cctgttccgg gatgttttcc
      601 aacagcacca tcagcaactg gcgttcgatc tcctgaatga catccatcat ccgcttaata
      661 acctgcccgg tgagatcctg aaaatcctgc gccatcatga tttccagcag ttgcgcgtta
      721 gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt taccagttca
      781 cgggcgtcgg caaggtcaat cggatcggca aaccagtcat cccaacgttg ggttaacgct
      841 tttgctgatt tctccatttg atcctgatgc ggttgtgacg cctcaacact gttcagcgcc
      901 cgctccgcag cctgggcggt catctgcaca acatagtaca aacgatcgcg cgcatcgggg
      961 atggcttccg ccgcttcggc aatggcctga accagcccca gttcccgcaa actgtcgcgc
     1021 agcatatgcg tcaggctgcc gatgcgcgca atgatatcgc cagctgaatg ctcgtcagca
     1081 ggtttgattg atggttgcat catagtcgca tcctcacatg cccagtttct caaagatttt
     1141 gttgagtttt tcctccagcg tcgcggcggt aaatggcttc accacatagc cactggcccc
     1201 cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct gcagtcacca ttaacactgg
     1261 caatgccgac atcgcgccat ccgcacgaat tgttttcagc aattccaggc catccatatt
     1321 gggcatgttc cagtcggaga taacaaatcc ataaccgcct gcctgcaact tattgagagc
     1381 gtcgacacca tcttccgctt cctcaacatt attgaatccc agctctttca gcaggttacg
     1441 cactatgcgt cgcatggtgg aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc
     1501 catttcacac tcctgattta aatacgtatc gcctgtccgg cactaattgt tgccaacatt
     1561 tgctggctta cctggctaag atcgaccact tcgcagacac cacccatatt gatggcctcg
     1621 cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg caagggtcca tgcccccgcc
     1681 tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca taccggtcag gatcacccca
     1741 accgcattac gccccgcctg tttggcgaca gaatggaaca acacatctac cgaaggccga
     1801 tgacggttaa ccgccgggcc gtcgtgaatt ttgatttgat aatttgcgcc actacgcgcc
     1861 agttccatat gccgatcgcc cggcgcaata taggcatgcc ccggcaagac acgttctccg
     1921 tcttcggctt ctttaacccc gatctggcaa agcttattaa gtctgtcggc aaaagagcgg
     1981 gtgaaaccgg gcggcatatg ttgggtaatt aacaatgccg ggctggaaag cggcaacggt
     2041 tgcagtacgt gacgaattgc ctcagttcca cccgttgaag caccaatcgc aatcagtttt
     2101 tcagaactca acaacggccc tgccttcagc gttgtcggtg ctgacaatgg cttatgtgct
     2161 gcaaggctcg cctttgctgc cgtacgcacc ttttcagcaa tcatttcgct atacgccagc
     2221 attccttcgc gaatacccag ttgcggtttg gtgacaaaat ctattgcccc cagctccagc
     2281 gcgcgcagcg tgacttctga tcctttgccg gtcaaggaag aaaccatcac aacgggcatt
     2341 ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg catttcaaca
     2401 tccagcgtca gcacatcggg attgaatttc ttaatcaagt cacgcgcaac cagcggatca
     2461 ggcgcggtcg ccaccatttc catgtcgcta tggctgttga tgatttctgt catgatctgg
     2521 cgcatcagtg ccgaatcatc gacagataac accctgattt tgctcatcgt taatccttac
     2581 ttagcgcata caccgtctga ccacgcagcg tgaagcggcg ctcgaggtgg ctaaagtttt
     2641 cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag cggcgcaaaa
     2701 tctcctgctg ggtagtttga tcgaagtaga tcatgacgtt acgacagaag atcgcatcaa
     2761 acggccccgg cacggtgtac tgtttcgcca gtaaattcag cggggcaaaa tcaacatagt
     2821 tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct cgcatgaaat
     2881 accgttgcag ttgctgcggc gtcaggtttt tcaactcttc atggcgatag ataccgcttc
     2941 tggctttttc cagcacttcg gtgtcgatat cactggcaaa cactttccag cgtccgggca
     3001 cggtgcccaa tgtgtcagcc agagtcatcg caatgctgta cggctcttcg ccggtcgaag
     3061 ccgccgcgct ccatacgcga tcctcgccag aacggcgacg tgcgtgatcc gcgagcagag
     3121 ggaaatgatg agcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa ttgataaacg
     3181 cctgccactc accgctgtgc tgattagatt ccagcaagtt cagataatga ccgaaatccg
     3241 tcagtcccag agaacgcaaa cgacgaacca gtcggttgta aaccatgtcg cgtttatggt
     3301 cagccagaac gatcccggct cgttgataga tcaattgact tatccgccga aaatgcgcgt
     3361 cggaaagcgc caggcgctcg gtcatctgta acaataaaga cgtttgccca cagggcagag
     3421 atgaagtcat agcgccttct caatcacttc aggataccac tggcgcaatt tgtaactgcg
     3481 ccgactcatg tcgtgctact tcatgttctt caagggtaaa taccgccaca cgcgacgaaa
     3541 gatggtcggc ctgattcgcc agttgttccg ttgccaccgc cgcttcttct accagcgagg
     3601 agttctgctg agtcacctga tccatctggc tgacagcctg tgcaacctgc tcaatccccc
     3661 gccgttgttc ttccgacgcc gaagcaattt ctcccataat gtcgttcacg cgggtcaccg
     3721 aactgacaat atcggtcatg gtcgcggcgg cgttattcac cagtttcgaa ccctgctgga
     3781 cacgattgac tgactcttcg atcagccctt tgatctcttt tgctgcctgc gcgctacggc
     3841 tggcaagatt gcgcacttca cctgccacta ccgcaaaacc acgcccctgc tctccggcgc
     3901 gagccgcttc cactgccgca ttcagggcca gaatattggt ctggaaagca attccgtcga
     3961 taacgctgat aatgtcgcca attttttgcg agctggtggc gatctcctgc atggtgtgag
     4021 tcatggtact gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt tttgccagtt
     4081 ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat tgctccatac
     4141 tggcggccgt ttgtgccagc gatgccgcct gctgttcggt acgacttgag agatcgttat
     4201 tgcctgcgac aatctccgcg atcccaatgt gcatctcatg gcttccctta cgcacatcac
     4261 tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct ggcaaaaatg gcggtgatct
     4321 cattacgacc atataccgca atcggacgcg ccagattacc cgcagcaatg ctgtcaaaat
     4381 ggctaccgat aatggccagt ggttgaacaa tcatcttgcg cgtccaccac agcacactgc
     4441 tgatgtagat cgccgcaaca ataatcatgc tgataaacac cagcgccgaa atctgatagt
     4501 tacgctggct ttgcgcactg gcggcttcca gcacatggtt gatctccagc tgccaggcct
     4561 caaagttaac gtcaaacgca ttctgtgatc cctgcaccgg cgcagtgagg aaatccgaaa
     4621 gttgattact ttcaagccag gtcgcctgat gttcgagatc gttgtgccag cgggcaaaac
     4681 ttttctccgt ctctttttgc aatgccctga cgtgctcgtt gcccgcagtc atcgccataa
     4741 aacttttaaa cagcgtggtg gattgcgtca gactggcgcg cgccgtcgtc atcaacgttt
     4801 taatgtcatc cgccggatag ctaagcgctg tcagagtgcc tgctttgttc agcgcggtac
     4861 tggcctgtaa cattaccgcc cgagtttgcg ctaatgccgc acgttgctga ttgctctgct
     4921 cgccgtgatt cagtcgttgc aaatcgtcgc gaaatgccca aaaagacatg ccgttactgc
     4981 caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac
     5041 gattaaacat caacgctccc catcaggcgg caatgaccgc gttagtaaaa actcgtcaaa
     5101 atgtttccca gtttggatct tgttcagtaa ttcgcagtcg tggctgtgcc ggtggttgct
     5161 cactggcagg acgggatggt gtttgcggtt tattggtgag tgggctggct gccagacgga
     5221 acgcggaaac cgcttgcgtt aaacgactcg cctgttcttc cagcgcagct gcggcggcag
     5281 ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg atccatttcc gaaaccgcca
     5341 atgcgacttg atcgatgcca cggctctgtt catccgatgc cgatgcaatc tcgcccataa
     5401 tgtcagtcac gcgagtgaca gcattgacga tattgttcat tgtttccccg gcgctttcga
     5461 ccagcaccga accggtatca atgcgcgaga cggagtcttc aatgagggct ttgatctctt
     5521 ttgccgccag ggcgctgcga ctggcaagat tacgcacttc acccgccacc acggcaaaac
     5581 cacggccctg ttcacccgca cgcgcggctt caaccgcggc attcagcgcg aggatattag
     5641 tctggaaggc aataccgtcg ataacgctga taatgtcggc aattttcttc gaactatcgg
     5701 cgatctcatg catcgttttc actacgccat ccaccacttt gccgccgtgc tgggcggtgt
     5761 cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg
     5821 tcgcggtgag ctgctccatg ctggcggcag tttcttccag cgcggatgcc tgctgttcag
     5881 tacgggagga aagatcggtg ttgcccgccg caatttcacg ggtaccggca tagatggcat
     5941 ctgaaccttc gcggacatga gtgacggtgt cagtcaaaga gcgttgcata tgtgaaacgc
     6001 tctgcgccag gtcacccatt tcactgcgcc cgtcaatggt cagggtattc gccaggttac
     6061 caccggcgat ttcgcaaatg tgagcaataa tttttgccag cggagtaagc aacatacggc
     6121 gaatgccgta ccacgccacc agcagaatca ataccaccac cagcgcgata accgccagtt
     6181 gccactgggc aaatcggtaa tcatctgcgt tgtcagtgac gatatcgcga tacagttttt
     6241 cactgctgag ggcgtactga gcaaacgctt cgcccattgc attttgcatt ccctgggttg
     6301 gctgagcgaa ataagctcca gtattgccat aatcaagata atcaatcagt tcagttaacg
     6361 ctgtgtaata gtttttatat ttttcatcaa aattacggct ggtagcgacc atttcaggta
     6421 acggtgccat gcttttgaat tttttataat gcgtcgcggc ctgggccaat gttttcctgg
     6481 cgctatcgag caattcaact ttggcgttac tttgttgatt ggaggaatcc atcatcatcc
     6541 gcaccgctga acgactcagg ttaatgcgcg tttgcagcat taaatcccag gttgacgtca
     6601 gctcgccctg ctgttcccgt aattgattgg aaaccacaaa gctcttctgg ctatggtgaa
     6661 gggaagaaaa aaacaaactg ccggaaataa gctgtaacag tgcgaatacc cccagcacca
     6721 ttaccaacag cgtgactacg cggatacggt taatcataag gcaccttcct gaaaacaagt
     6781 tgatctcgtt atcggcaaga aggggggaaa ctttattgct gatgccaccc gccgcgaaat
     6841 tgaaataaaa aacccgatgc gcagatcatc gggttcattt caattgagga aatcgggaga
     6901 attacgccac ttctgacgcc gcgctatcta acagcgccat ctcttcgctg ttcagcagtt
     6961 tttcgatgtt caccagaatc aacatacggt cgcccagtgc gcccagtcca gtgagatatt
     7021 ctgttgaaag cgtcacggca aactctggtg ccggacgaat ttgctccgcc gtcaatgaaa
     7081 gcacgtctga aacgccgtca accacgatgc cgaccacccg ctgtccgaga ttcaggacga
     7141 taactaccgt gttgtcgtta tagtccacat ccacctggct gaacttaatt cgtaagtcaa
     7201 caatcggcac aataacgccg cgcagattcg tgacgccttt gataaacgct ggcgtgttcg
     7261 caatccgtgt tacctgatcg tagccacgga tctcctgcac tttcaggata tcaataccgt
     7321 actcttcatc accaagggta aataccagaa attcctggcc tgacggctcg ctggccagct
     7381 ttgttacatt cgtcataccg gtcatattgt taccttttta ctcattcagg cggcggtgtt
     7441 cgccatacgt tgttcgcggt ttatcgcctg caaggcggag acatcaacaa tcagtgccac
     7501 gctgccgtcg ccaagaatgg tcgcagcaga aatgccgggg actttgcgat agttactttc
     7561 aaggttttta accacaacct ggtgttgacc aattaattga tccaccagca aggcgtagcg
     7621 gcgaccgcca ctttgtaaga tcaccacaat tccctgggtg gcttcggttt tcgcgcccgc
     7681 gacgttgaac actttccaca gttcgacgat gggcagatat tcaccccgca cttccagcac
     7741 ccgctcgccg ccggccagtg gatggagatc ggcttcacgg ggttgcagtg attccataac
     7801 agcattcagc ggcagaatga aaacttcatc cgcaacgcgt acggacatgc cgtcgaggat
     7861 ggccagcgtc agcggcagta aaatgcggat cgtagtgcca gtaccctgct tcgactggat
     7921 ttcgacatga ccgcccatct cctggatatt acgtttaacg acgtccatgc cgacgccgcg
     7981 cccggagacg tcggtgacct gctctgccgt ggagaagcct ggtgcaaata tcagcattgc
     8041 aacttcgtcg tcgctcatgt tttcgctgac agtcaaacct tgcgaggccg cttttgccag
     8101 aattcgctca cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc
     8161 ctgatgttcg gcagacagaa ttaaatttcc gacgctgttt ttacctgcgg cgagccgttt
     8221 ttctggcagt tcaataccgt gatcgaggct attgcgtacc aggtgggtca gcgggtcgat
     8281 aatgcgttct atcaggctct tgtcgagctc ggtggaactg cccaccagcg tcagttctac
     8341 ctgcttgccg agttttcccg ccagatcacg caccagccgg ggatagcgac taaagacata
     8401 ttccatcggc atcatgcgaa tcgacatcac tgattcctgc aaatcacggg cgttacgttg
     8461 taactgcccc atgctggtaa tcaaatcacc atgattaacc gggtccagtt cgctggaacg
     8521 ctgggcaagc atggactggg tgataaccag ctcgccgacg aggttaatta attgatcaac
     8581 cttttctacc gctacacgga tgctggtgga ttcactgctg cgcgtcgttt tttcccgctc
     8641 cacgcggccg gttggcgctt gttcggctgc cagtttaagc acaggtgggg tagctatttt
     8701 tggcgaaact tctactgttt caaaggtaat ctgatcggct tcaatcacaa aacagagtac
     8761 cgctgtgatg tcatcttcgg cgatatcgcc cggtaatatt gccgagagag aatccgcccc
     8821 tttcaccacg tcatttaacg ttgtcagatg ccccagctct tcttccagca ggtcgacttc
     8881 gccggccttc aggcgcgaaa ggataattcg tcgcggcaac tgactgcgac tttgctcatc
     8941 ttgcggttca cttttggcaa ccacacttaa tcgggtcact gcggatggcg tttcgccttt
     9001 cgcttctaat gccagttgac gcaaggcctg gcagatataa tcgtagctgg cggcatccgg
     9061 ctcttgcgac tgtttataag cgtcgagctg ttcttgcatg atgtccttcg tttccaaaaa
     9121 cagattgata atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt
     9181 ttccatcaga tgcgtggttt cctgcaaaac gctgaagcca aaagttcctg cccctccttt
     9241 gatcgagtgg gcagcccgaa agatggcatt caattgttcg gcatctggcg cttccggctg
     9301 caaaaccagc agatgctgct ccatatcagc caacagttcg tccgcttcat caaaaaatgt
     9361 ctgataaaaa tcgcttatat cgatgctcac gctgtcacct cggttcggct gatggcattg
     9421 tgggaacact gacctgtggt gcaacctcag gtttttctag ggcgcttact ggctcattct
     9481 ggctttcggc gttttcatgc aaaatggcct gttcggcttg tttgttcagt accaacaggc
     9541 tgatgcgacg gttgacggca tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca
     9601 tgccgacgac acgtaacact ttgccgctat ccaaccctcc gaccatcagt tcgcggcggg
     9661 atgcattggc ccgatcggca gaaagctccc agttgctgta acctttctca ccgctggcgt
     9721 agggaaaatc atcggtatga cctgaaaggc taatacggtt gggaataccg ttcagcacag
     9781 gcgcgatggc gcgcagaatg tcgcgcatat agggttcgac atcggcactg ccggttctaa
     9841 acatcgggcg attctggcta tcgatgatct gaatacgtag accttcctgg accagatcga
     9901 ttttgagatg gagacgtaac gcccgcagtt tcggatcgga ctctatcaac tggtcgagat
     9961 caccccgcaa tttccgcaat cgactttgct ccatgcgttt tttcagctct tcgatgttcg
    10021 gctgcttatt cacttccccc tggctttggg tgtaatcatc accaccgccg ggaattgggc
    10081 tttcactatt ggaaatgcga tcgccgcccg taaccgctgt cgccagtgga gtccggaagt
    10141 actccgcaat ctgaatcaac tcttttgggc tggagatgga gatcagccac atcaccagaa
    10201 aaaaggccat catcgcagtc ataaagtcgg cataagcaat cttccacgat ccatgtgctg
    10261 ccccgtggct tttggctttg cgtcgtttga cgacaataat cggatgcgct tgattcttca
    10321 tgcttcctcg gttgtcgtct gttgttgcgg atttttcacc gcacgcacat gctcttccag
    10381 ttcaataaac gacggacgtt cgctggaata gagcgttttg cgaccaaact caacggcgat
    10441 aggcggtgcg taaccgttca gattagaaag cagagtgact ttgacgcact gcatcatttt
    10501 gctggtttcg gcgcttttct gacgtaaaac actcgctagt ggggaaataa atccgtaagc
    10561 caataaaata ccgaggaaag tccccaccat cgcatgtgcg ataagcgccc ccagctcggc
    10621 ggcaggacga tcggctgaac ctaacgcgtg aacgcccccc attacagccg cgacaatacc
    10681 aaacgccgga agtgagtccc cgaccagcgc cagactgttc gccgggactt ctgcctcgct
    10741 ttcgtgcgtc tcaatctctt catccatcag ggcttcgatt tcgaaggtgt tcatgtgacc
    10801 gctgataatc aggcgcagat aatcgacgat aaaatcaagc atgacgctat ccgcgaggat
    10861 gcgtgggtag ctggcgaaga tctcgctctc acggggattt tcaatatcac gttccagcga
    10921 aaacatcccc atctgccgcg atttcgccat caaccgataa agcagagcca gcagatccat
    10981 atacattgct ttggtgtatt tggagcgacg aaacagcaac ggcagcgctt tcagcgtgcc
    11041 tttaatcgct ttgccattat tgccgacgat aaacgaccca atccctgcac cggcaataat
    11101 caccagttca gcgggttgat agagtgctcc aaggcttcca ccggtcatca aataaccgcc
    11161 gaaaactgta ccgagaacaa ccaggtaacc taataagata agcacgacat catccttcca
    11221 ctgttgacca tgacaggatg ttcagtcgtc aggcgttaac gcgcgattgg ggaaaaaaaa
    11281 agcagcggta cgtcgttacc gctgctggaa tgttgcgcca caccgtatca gttaaacagc
    11341 ctgtactctc tgttcatcca gcagttgtgg gataatatcg gcaggattct gggaaagttt
    11401 acgtcttttt actgcccggg atggcggttg acataagctg caggcaaagc tgccaacagg
    11461 ctggtgagcg tgggtaataa aattgccgcc gcagcagttg cagctggaaa gttgcagtaa
    11521 tccactttca acaaaccgca ccaatgtcca ggcacgggtt aatgccagca gtggtccttc
    11581 ttctgcttgt gggcactgtt caaggtataa acggtaggct ttgatcaccg catcgacgcc
    11641 attacacaaa ccggttttca gtaaaaactg ccatgcatta cagaacatcg aagcatgaac
    11701 gttttgttcc caggtcataa accagtcggt tgagaatggc agcatgcctt tcggcggtgg
    11761 gcttccgcgc agttctttat aaagttttat caggcgtccg cgacttaact gtgtttcgct
    11821 ttccagcatc tgcaaacgag cgcccagggt gatcaattcc attgccagct gaatatcccg
    11881 cgcttcctga acaatgcttt tttcactcat gatcaggccc ttttcttgcg cagcgcttct
    11941 tcaggctgat taacatcatt cagcaagcgt gttgagagca tgatgccggt atgaatttgc
    12001 tggagatcgt caacgcggga atcttgcgtc aactgagtaa tcgtctggtg gctgtcaaaa
    12061 cggaagtgac aaaccagctg attggtttct gccagcttaa ccatttgcgg aaga
//
LOCUS       DAETQL010000109        11925 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.109, whole genome shotgun sequence.
ACCESSION   DAETQL010000109 DAETQL010000000
VERSION     DAETQL010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11925
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.109"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..2340)
                     /locus_tag="K0W65_003444"
     CDS             complement(<1..2340)
                     /locus_tag="K0W65_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBI3096572.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2274..2897)
                     /locus_tag="K0W65_003445"
     CDS             complement(2274..2897)
                     /locus_tag="K0W65_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HBI3096573.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2894..4582)
                     /locus_tag="K0W65_003446"
                     /pseudo
     CDS             complement(2894..4582)
                     /locus_tag="K0W65_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4731..7358)
                     /gene="gyrA"
                     /locus_tag="K0W65_003447"
     CDS             complement(4731..7358)
                     /gene="gyrA"
                     /locus_tag="K0W65_003447"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBI3096574.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7505..8227
                     /locus_tag="K0W65_003448"
     CDS             7505..8227
                     /locus_tag="K0W65_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBI3096575.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8355..10289)
                     /gene="yfaL"
                     /locus_tag="K0W65_003449"
     CDS             complement(8355..10289)
                     /gene="yfaL"
                     /locus_tag="K0W65_003449"
                     /inference="COORDINATES: protein motif:HMM:NF007285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HBI3096576.1"
                     /translation="MMEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMA
                     DSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGD
                     GATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATD
                     GVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGT
                     LLFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQ
                     NNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDP
                     TPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHY
                     TAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADN
                     QNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLE
                     AGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTA
                     VGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAW
                     QKGSDDFAQTAGFLSMTVKW"
     gene            complement(10299..11894)
                     /locus_tag="K0W65_003450"
     CDS             complement(10299..11894)
                     /locus_tag="K0W65_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HBI3096577.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2757 a         3308 c         2945 g         2915 t
ORIGIN      
        1 ttcgacattg accagttcac caggctggta atgggttttg tccgttttca cccggatatc
       61 aagctgagga acggcaactt tgatcccggc gttctgaaaa ctgtactgac cattacgggt
      121 atacagcacc gaaaaagtga tgttaggcgc aaaggaattg ctcactggta cccgggcttc
      181 atacagggta tcgtttaaac gttgtagcgt tagccagttt gccggatgcg aaagcagcga
      241 ctgctgttcc acgcgatcgc gttccagcgt caataacgct tcatcaattg gctccgggaa
      301 agtaatcagc atcttcgcgg tttcgcctgg ctggtacagc gttttatccg ccacgatatc
      361 taccgtacca gtatgcgccg tgctgccctt accgctgacg gcatgactta acccagcgag
      421 aattaagccg tctttatcgc gtaatgtcag attgtagttg ccaggtttag cgaaattgac
      481 ggtaaaggat ttgccgcctg acggtagctc tccgctatgg ctcgtgcggt cttcgagacg
      541 caaccattca tacgtaacag gaacctgttt tgaagattcc agcgcggcat aacggaacac
      601 aaccgactcg ccgctattac tgtattgtgc ggcggtactt aaagagtaat gcgccaggcc
      661 gcgttcaatg aggatctctt tggtggtggt gacgcgatac gccgcgccgt cactggcgga
      721 gacggttaac aaatagcggc tcggtttatc ggcggcgggg agatttaacg ccacatgacc
      781 gctggcgtcg gacaccgttt cgctgccttc cagcgacacg gggaaacgtc cggcataacg
      841 caaatcgtta ccgaccattg ataattgctg agcgcgcaaa cttaactgca cgcgggcatt
      901 ttttaccggc tcgccatccg ggtagagcag ttgcagtttg ccgctgaccg cttcgccagt
      961 tttgaactct tttttgtcga gagctaaacc aatctcgaaa tgtggcttga tgtagtttgc
     1021 cacgcgaaaa ctgctgctat agacctgatt gcggtaagca agacgtaact cataacctcc
     1081 ggcaacggca ttttctggca ggcggaaact tccctgcccg ccattgcgcg catccagcgt
     1141 gacatcgacg gtttgcaaca gactgccgtt agcgtccagc accgaaagct tcgccggggc
     1201 gctgacgatg ggggatgaat gcaacggatc gcggaactcg cggcccatca ctttaacatc
     1261 gacacgatcg cctgcgcgat atagcgggcg atcggtaaaa atatacaagc gggtgttgta
     1321 gatttcgctt tcgtagaaga agttctcgga gacaaaaacg ccgccttcag catccttacc
     1381 cagaatgtat gaacgttctg gcgatatatg ttgtaactgc aaagtaccgc tgtcatcggt
     1441 caccccgcgg gtcatcacgc caagaccttc agtccacaag atctctgagc cgggcttcgc
     1501 ttcaccctgt tttttacccg cggtccatac cagaagctcg ttacctgaca ctttgctaag
     1561 cgccacggta tcggaaacaa acaccaccgt cgtcgcccga tacccaccta ccatcgcctc
     1621 gacgaggtac agccccggct cttgttggcc gagaggaata taaatattac ccggctgcgg
     1681 cgagatgaaa ttgctggatg cgccttccag ttttacccct tgctgcggct cgaccggttt
     1741 agcctgccat agtggataac ggaactgttc caccaaggga tattttttca gcggggaaaa
     1801 ctggttgttc tgtacataac gggaaggttt aataatggca ttgccgagat gtaattcggg
     1861 taatgcctga gtcacattct gccgtgactg agaagagaaa gtacgctgca tcacgcggcg
     1921 agatttgccg taccagttat cccacagcca ggtcagcgta ttgttcagcc cgtcgcccag
     1981 atattgcggt tgcaccacaa tgcgatgcag gtttttctgc tggcgcaaaa atgccatcgg
     2041 gtcaggaata cgatataggc gaacgtccac gccgccgtac tcttccatct gatagcgccg
     2101 ataatcacgc cccggcgctt ccagtcgcac tttcgcctct tcactgctgc taaaactgct
     2161 gtcagcaagc aaaaagaatg ttcccccggc gggcggcgca tagttactgg aaggaagcga
     2221 atcatcagct ttagcaagcc ctgttcccac aagagacagg gacaagcacg ctgctatcgc
     2281 gccagaaaat ttaaacgata aatgccaatg aaattgggat tggaatcgtt gggtatccat
     2341 cgggtgtcct tccatgtcat aagttgttgc agactgactg cgcgcattcc gttgtcagtt
     2401 ttcgtggcgc ttccggtgtg gtagatgacg taacgcccca tccagaccat taagtgctgg
     2461 gcatcgccct gatcgaaaaa aatcatatcg ccaggcagcg cctggtttat gtcttggcca
     2521 ataaactggc tgttgtactg aatcaaatta atcgcggtca cgtaggggcc gtttttcccg
     2581 ctcccctgat tccagttttg cgccagttga cgctgttcag gtgttagcgt catctctggc
     2641 ggcaaatact ggctagataa accgttactt tttagccatt tactgtcgtg aactttcagc
     2701 gtctcgttcg ccgcaaatcg caccaggccc gcacaatcct gctgatacca gcgtggactt
     2761 ggcccctggc ggagctgttc ttgtgcaatg cgtacaaacc aggcgcgaaa cagcctggat
     2821 tgttcgacat tcagcatttc gctatgggca acaacacaac acagccagca aatcagcgcc
     2881 agcagcccgt gtctcatagc ggctgccagg ttatgggtag ccactgccag gcgacaccgg
     2941 gttccatctg ggccagcttc atgacataac gcggttgttg agataaagcg tccagcttcg
     3001 gcattaataa agtttgtgcg gcgttataaa aaaccggttc gagattcttc ggcagactgg
     3061 tcaacgtttc gttacgcagc agtttcgcca cgccttgtgg attgatatag agcggaacga
     3121 tgccatcagt gggtattaca tccaccattg ccgggcgggt tttattcagt gtttgcaggg
     3181 cgttattaac cagcgtgtca tcgagggaga aaagcagcgt tttgttttgc atcgccagcg
     3241 acacacggaa aaaataatcc gacattaatt gatcgggttg cgccgcctgc gctttcggat
     3301 actgaccgta tcgggaactc acttcgcgac accagatttg cgcttcgccc tgctgagtct
     3361 ggcttaccgg caaaacacct tctggcgctt tgctttcgtg cgcaccaata ttttgcgtaa
     3421 acagtttccc tggcaattgc gcctgttcgg cagtgccatc aaactgaccg acaaacagcg
     3481 gggtttgcaa ttttgagtct tcataccagc acagccccgc ggcaccgtct aacgccccat
     3541 tcagcttgtc gttttcctgg ctgatgtgcg aaagcatctc ttcggcaata ccgtgtgaat
     3601 acggcaccgc cacacagaag ctggctccgg caggcatact gttccagacc ggcgtaaaat
     3661 cgaaactggc atctacgctg gcggattcat catttaacgc cacaaaactg tgccagccgt
     3721 cgttacccat ttcgaagcgt acgccagcaa aagaaggcat taaccgctgg tagccaaaac
     3781 ccagcaacct ggcgctgacc acgatgcgca ggcgtactga cgttttttcg gctgtacgct
     3841 cttccaggcc aaagctggct tgccagcgtt ttttaccgct caacaaatca cttgcgatcg
     3901 ccgtggcttc ggtatcctgc tgatcatctt taaacaacat atccgtactg gaaaacacca
     3961 gcatcttgtc ctgatatgtc gcgaacatca gggcgttatt gccgttatag cgcaactgat
     4021 aaacaggaat agtttcacta tttatcttga tgctactgat ttccgttttg cttaactagc
     4081 tgtcgctggt agcggcaaac aacaatggct ccagcagttt gcttaaacca ctgcgctgga
     4141 tcagcaccat gtaatgtgaa agatggcctt gtttatcgtg ccatagcgcc gcctgcgcgg
     4201 gctgatctaa gagtgacgaa aagagcttat ctttcagcgt gagatcgtgt tcatagacaa
     4261 tgcggcgaat gctgccttca atgcccagac gatcggcatg attctgataa tagaaaacga
     4321 aatcttcgct cagaacatcg tggagaaacg gaatggtgag gagatctttg ggaagctggc
     4381 tcagagagtc gctgtctaga aagaggtccg gctcattgag atcgatttgc agattgttgt
     4441 gcaccaccag cggcgacaac gttttttctg gcccactgcc agcatattgc aacgcccaga
     4501 cgccagcgga aagcagtgct attgcgccaa aacctacaag accatagaac cgccagcctt
     4561 tcgccttttt ttcaccactc attgccacat tccttgtgta tagccagcca ttttttacgg
     4621 gcacagccaa actttaccgc gccctaatac gacaaaagcc cagactttgc agcctggact
     4681 tttcaattca aacaagggag atagctccct tttggcatga agaagtaaaa ttattcttct
     4741 tctggctcgt cgtcaacgtc cacttccgga gcaatttcat catccccttc cgcggcactg
     4801 ccgtcgatgg tatccagatc ttcctcgtca accggttcag caacacgctg cagacccact
     4861 acgttttcat cttccgcagt acggatgagg atcacgccct gggtgttacg gcccacgatg
     4921 ctgatttctg aaacgcgagt acgtaccagc gtaccggcat cggtgatcat catgatctgg
     4981 tcgcagtcat ctacctgcac cgcgccaacc actaaaccgt tacgttcggt aaccttgatg
     5041 gagataaccc ctttcgtcgc acgcgacttg gttgggtatt ccgccactgc agtacgttta
     5101 ccgtaaccgt tttgcgttgc ggtgaggatt gcgccatcgc cacgaggcac gatcagagag
     5161 acgactttat cgccttcacc taagcgaata ccgcgcacac cagtggtatt gcagcccatc
     5221 gcacggacag aagactcttt aaagcgcacc actttacctt cggcggagaa cagcattact
     5281 tcatcttcgc cactggtcag atcaacgccg atcagctcat cgccgtcaac cagtttgatc
     5341 gccactttac cggcggtacg cagacggttg aattcggtga ggacggtttt cttcacagta
     5401 ccgttagcgg tcgccatgaa gactttcacg ccttcttcaa actcggtcac cggcaggatc
     5461 gcggtgatac gttcgtcctg ttccagcggt agcaggttga cgatcggacg accgcgcgcg
     5521 ccacgagtgg cttccggcaa ctggtaaact ttcatcgaat agacgcgacc acggctggag
     5581 aagcacagaa tatggtcgtg cgtgttcgcc accagcagtc ggtcgataaa gtcttcttct
     5641 ttaatacgtg cggcagattt acctttcccg ccacgacgct gcgcttcgta ttcagaaagc
     5701 ggctgatatt taacgtagcc ctggtgagag agcgtcacga ccacatcttc ctgggtgatc
     5761 agatcttcca ggttgatgtc tgcgctgttg gcggtgattt cagtacgacg tttgtcaccg
     5821 aactgttcac gaaccagctc cagctcttcg cggatcactt ccatcagacg atcggcgcta
     5881 ccaagaatac gcaacagttc cgcgatctga tccagcagct ctttgtattc gtcgagcagt
     5941 ttttcgtgct caaggccggt cagtttctgc aaacgcagat ccagaatcgc ctgagcttgc
     6001 tgttcggtca ggtagtacag accatcacgc acgccgaact ctggctccag ccattccgga
     6061 cgcgcagcat cgtcgccagc acgttccaac atggcggcaa cgttgcccag ctgccacgga
     6121 ttagcaacca gcgcagtttt cgcttctgca ggcgtcggcg catgacggat cagttcgatg
     6181 atcgggtcaa tgttcgccag cgccacggct aatgcttcaa ggatatgagc acgatcgcga
     6241 gctttacgca gttcgaaaat agtacgacgg gtcaccactt cacggcggtg acgaacaaac
     6301 gccgcgatga tgtctttcag gttcatgatc ttcggctgac catggtgcaa tgccaccatg
     6361 ttgataccga aagaaacctg caactgggtc tgggagtaga ggttgttgag cacaacttca
     6421 ccgaccgcat cgcgtttcac ttcaatcacg atgcgcatac cgtctttgtc agactcgtca
     6481 cgcagcgcgc tgatgccttc cacgcgtttt tcttttacca gttccgcaat cttctcgatc
     6541 aggcgcgctt tgtttacctg atacggaatt tcgtggacga taatggtttc acgtccggtt
     6601 ttggcgtcaa cttccacttc agcacgggcg cggatatata ccttgccgcg accggtacgg
     6661 taagcttctt caataccgcg acgaccgtta atgattgccg ccgtcgggaa gtccggcccc
     6721 gggatgtgtt ccatcagccc ttcaatgctg atgtcttcat catcgatata cgccagacaa
     6781 ccgttgatga cttccgtcag gttgtgcggc gggatgttgg ttgccatacc tacggcgata
     6841 ccggaagaac cgttcaccag caggttagga attttggttg gcatgacgtc gggaattttt
     6901 tccgtgccgt catagttatc aacgaaatcg accgtctctt tttcgagatc ggccatcagt
     6961 tcatgggcaa ttttcgccag acggatttcc gtataacgca ttgccgccgc agagtcgccg
     7021 tcgatggaac cgaagttacc ctgaccgtct accagcatgt aacgcagcga gaatggctgc
     7081 gccatacgga cgatcgtgtc ataaaccgcc gagtcaccat ggggatggta tttaccgatt
     7141 acgtcaccaa cgacacgggc agatttttta taggctttgt tccagtcatt gcctagtacg
     7201 ttcatggcgt aaagtacgcg acggtgtacc ggcttcaggc catctcggac atctggcagc
     7261 gcacggccaa caatgaccga catcgcataa tccagatagg agctctttag ctcttcctca
     7321 atgttgaccg gtgtaatttc tctcgcaagg tcgctcatct aaccgctatc cctctactgt
     7381 atcccggatt caaaggtcgc aaattataac acagccgcgc agtttgaggt aaacctatac
     7441 gctttattca catccaatgc ctgatatact cgtttgtctt gccaattacg gagtagaagt
     7501 gccaatgaat gccgaaaaat cgccggaaaa ccataacgta gaccacgaag agatcgctaa
     7561 atttgaagcc gtcgcctccc gctggtggga tcttgaaggt gagttcaaac cgttgcaccg
     7621 cattaacccg ctgcgtctgg gctatattgc cgagcgtgct ggcggtttat ttggcaaaaa
     7681 ggtgctcgat gtcggttgtg gcggcggcat tctggccgag agtatggcgc gcgaaggcgc
     7741 gacggtgacc ggtctggata tgggctttga gccattgcag gtggcaaagc tgcacgcgct
     7801 ggaaagcggc attcaggtgg attacgtgca ggaaaccgtg gaagagcacg cggcaaaaca
     7861 tgccgggcag tatgatgtgg tgacctgcat ggagatgctg gagcacgtac ccgatccgca
     7921 gtcagtggtc agagcctgtg cgcaactggt gaaaccaggc ggcgatgtct ttttctcgac
     7981 gcttaaccgc aacggcaagt catggctgat ggcggtggtt ggcgcggaat atattttgcg
     8041 catggtgccc aaaggtacgc atgatgtgaa gaagtttatt aaaccggcag agctgcttgg
     8101 ctgggtggat cagaccagtt taaaagagcg acatataacc gggctacatt acaacccgat
     8161 cactaattcc tttaagctcg gccccggcgt ggatgtgaac tatatgctgc acacgcagaa
     8221 taagtgaggt tgatgtttgg ccgcgccaat gcctgatgcg acgcttgcag cgtcttatca
     8281 ggcctacaaa tgctctccgt aggccagata aggcgtttac gccgcatccg gcaattgtga
     8341 tggtcagaca gcgattacca tttcaccgtc atcgacaaaa accctgccgt ctgggcaaaa
     8401 tcatcactcc ctttctgcca cgccacgctg ccgcgcaggg acactcgctg actgatattg
     8461 cccgtgactc ccacttttat ttcaccccgt agcttcaccg catcgtcact gatagtgctg
     8521 ccatcttctt caatttccgt cgcatgggga tcgtgatagt aattcagatc taatgttggt
     8581 gtgacaccaa ccgcggtacg ccattcgctg tgtaaaccca gccgcgtctg aatatcatcc
     8641 ccctgcgatt gtgacacgcg cgcatggtta gcggcggtaa aatcatcctg ctgcacgccc
     8701 tgataaatca cctgcgcctg cggttcaatc accacaccac gccccggtaa ccactgatac
     8761 ccagcttcca gcgaggcgat aatccccgac gaatggtaac gatccacgcc atcttcatgt
     8821 tcggaaacat cattgttaaa ccacgcgtat tgcagccagc tatccagcca ggccccctgc
     8881 ttctgattac cgtgctgata ccagcttgag gtcagcccaa cggcataacc gtggttctgg
     8941 ttatcggcgc gagttccggt catattcgag cggctgtcgc cctgattatc actgtagcca
     9001 ccaaccgccc caagcatcca ctcgccatcc gtgccccagc gcccgctaaa caagccgccg
     9061 ctaagctgca ccgtagaagt gtcttcctgc tgagccagtt gccctgccgc cgtgtaatga
     9121 taacgtccgc cgataacacg taaattcagc gtctgaccat cgccaccagc gtgatcgcgt
     9181 cgctccatca taaacgcctg atttgccgcc cgcaggttat tgagataacc gccaactttg
     9241 gcattcagca ccggctggta agcaggcgta ggggccgggt cgggggttgg gtcaggatcc
     9301 ggcgtgggat caggatcggg agtcgggtct ggctcaggtg gcgatggcgg cgttacttct
     9361 tgcgatcgca gataccagtc gttgttatct tccactagcg tgtagtcata cgctcccatg
     9421 ttgacgtagc cactgccagc cagactgaac tgtgcattgt tttgaaactg cgtgggatca
     9481 gctgcgaaat caaccacttt aatgcctgtc gatgtcggct caccaatccc tgtaatggag
     9541 ttaaccacca cagttgtgtt gccagcagta ttaccgttca tcaccaattg atcgcttacc
     9601 gagtcatcgc cgtttaattc gctgtcgaat agtagcgtac cgccgccggt ataatcaccg
     9661 tttaccgtca gcgtatcgcc agcgacgcca ttttgcaggc taaccgcacc ggacgtattg
     9721 gtcagatcac cattaaccgt cacgtcagca agataggtca ggctgtcagt ttcaaggttg
     9781 gtattaagct cgcctgtcaa cgtcacgcca tcggtagcat ttaccagcgt cccatcgccg
     9841 ttaaatagtg aggcattaag ggcgacggaa gtatcctgcc cggtcagacg caaaaccgta
     9901 ccatcgctga tgtcgatagt gccgctggaa gtagcatcaa ttgactgaat atcctgatcc
     9961 gcgccagtaa cgaacgttgc cccatcacca acccacagtg acgaagcata aggaaagata
    10021 tccgcaacat cgcctttcag ggtgccttct tcgacacgta ccgccgactg cgttgtaccg
    10081 ctggcggtca gctccagcgt acccgccccc gttttagtaa atgtgctacc ctcatcctga
    10141 tgctgcccgc tgctgtcagc catcagtgcg ccccactgcg tgtctacccc cgcatcaact
    10201 gccacttcac cgtcggcgcg catttcaata tcacgcccat gctcagtcgt cgtagagcga
    10261 gtatcgacat tggcagtaat ctccatcacg ctttcttgtt acttatcggt aaagataacc
    10321 tggcggttat agtgggtatc tccaagctgc gagttgtcgc tcaccatcag cgtaccggag
    10381 gcgatttgtg ttgtgcccaa ataactatta ttgtttgcca gacttacctg cccgtcgctc
    10441 gcatcgacca ccacgtagac gccatctttc ccgtcgccgc cgatatgcaa attgtcgcca
    10501 cctgcaagat ctaccacatc ctgactgccg ataaccgttc cgctgctgcc actgacttca
    10561 aggccatcat tgtatgatgt gccgctctgc caagtggaga aatcagagag atcgagcaca
    10621 ccgccgttta acacgatcga ctgcggatcg tcctggagag cggtaagatc tgccgcgtcg
    10681 ccttccagcg aaagcaccgc accatcatca acgactatat cgccggttag cgccatcgac
    10741 tgcgcccctg ccagcacgta gctgccgttt tgcgcaatgg tgagctgacc agcaccttcg
    10801 atgatgccag caaaactgcc ctgattaaca gtaacgttgc caccagcatc gatatttaaa
    10861 gtgccattct gaaagcccgt caatgcgtgc acaaaagttt gttgggtcga gccaacgttt
    10921 agctcagcct gattctgata ctgatcaata ctccctatcg tcagaccgta gcagtcttgc
    10981 ggatcgtcct ggcaatgcgt atcgccgaca ttcatcaggg agttgctgcg acccagggta
    11041 acttcaccgt tttcaatctg catctcacca gtaaagtcat tgttatctgc attaagtacc
    11101 agatcgccgg aacctgtttt ggtgattaac ccggtaccag caatagagtc aacagctccg
    11161 tcattctctg tattgccgat aaccagcgtt tttccgtcgg caatatcaaa ggtaacttca
    11221 cttaagccga gatacataaa gccacccgcc gcagtggagg gaccatctcc atagcctgct
    11281 gcgctattgt tctcatcgac taacacgccg ccgttctggc tgtagctgtc atcaacagaa
    11341 atatcaataa gatagggagc cgtcgcgcta ttggtatata tcgccccgcc ataaccttcg
    11401 gctgtgttat ttgtaaaggc ggtgttattt attatcgtat aacctgaagg atgcttgctg
    11461 tcgctattat tatcggtaac atcgattgcc ccgccatcgc catcactgta acttgttgat
    11521 gtatatgcct ggttattgtt aaaaacatca tcacttaaat agacgtcatt attaatggta
    11581 taaattgcgc cacctttgcc atcattagcg atattattga tggcgttagt gacacgtaaa
    11641 tcgacggcac cagtatcatt agtaccagaa gaatagattg cgccaccata gccgcctgcg
    11701 acgttaccgg aaaaaataac atcagtaaga ttcagcgttg agttttcttt agcaaatatg
    11761 gccccgccgt tattatattc tcctgtaacg gtgttattgg caaacagagt cattccagtt
    11821 tcattttctg gtaatagtga aaattccgct ccttgttgca aaaatacggc cccaccgctg
    11881 gcattattgg tcatatccga aaaaaccagc cattgcccat cggcg
//
LOCUS       DAETQL010000110        11685 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.110, whole genome shotgun sequence.
ACCESSION   DAETQL010000110 DAETQL010000000
VERSION     DAETQL010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.110"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..349
                     /locus_tag="K0W65_003451"
     CDS             <1..349
                     /locus_tag="K0W65_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBI3096578.1"
                     /translation="SGNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRL
                     QQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQ
                     NDLLLRATGGVCV"
     gene            complement(466..741)
                     /gene="yjfN"
                     /locus_tag="K0W65_003452"
     CDS             complement(466..741)
                     /gene="yjfN"
                     /locus_tag="K0W65_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBI3096579.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(890..1219)
                     /gene="bsmA"
                     /locus_tag="K0W65_003453"
     CDS             complement(890..1219)
                     /gene="bsmA"
                     /locus_tag="K0W65_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBI3096580.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1401..2150
                     /gene="yjfP"
                     /locus_tag="K0W65_003454"
     CDS             1401..2150
                     /gene="yjfP"
                     /locus_tag="K0W65_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBI3096581.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2147..2902)
                     /gene="ulaR"
                     /locus_tag="K0W65_003455"
     CDS             complement(2147..2902)
                     /gene="ulaR"
                     /locus_tag="K0W65_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBI3096582.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3010..4074)
                     /gene="ulaG"
                     /locus_tag="K0W65_003456"
     CDS             complement(3010..4074)
                     /gene="ulaG"
                     /locus_tag="K0W65_003456"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBI3096583.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4429..5826
                     /gene="ulaA"
                     /locus_tag="K0W65_003457"
     CDS             4429..5826
                     /gene="ulaA"
                     /locus_tag="K0W65_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HBI3096584.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5842..6147
                     /gene="ulaB"
                     /locus_tag="K0W65_003458"
     CDS             5842..6147
                     /gene="ulaB"
                     /locus_tag="K0W65_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBI3096585.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6157..6621
                     /gene="ulaC"
                     /locus_tag="K0W65_003459"
     CDS             6157..6621
                     /gene="ulaC"
                     /locus_tag="K0W65_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBI3096586.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6635..7285
                     /gene="ulaD"
                     /locus_tag="K0W65_003460"
     CDS             6635..7285
                     /gene="ulaD"
                     /locus_tag="K0W65_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBI3096587.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7295..8149
                     /gene="ulaE"
                     /locus_tag="K0W65_003461"
     CDS             7295..8149
                     /gene="ulaE"
                     /locus_tag="K0W65_003461"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HBI3096588.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8149..8835
                     /gene="ulaF"
                     /locus_tag="K0W65_003462"
     CDS             8149..8835
                     /gene="ulaF"
                     /locus_tag="K0W65_003462"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBI3096589.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8964..9239)
                     /locus_tag="K0W65_003463"
     CDS             complement(8964..9239)
                     /locus_tag="K0W65_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBI3096590.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9566..9961
                     /gene="rpsF"
                     /locus_tag="K0W65_003464"
     CDS             9566..9961
                     /gene="rpsF"
                     /locus_tag="K0W65_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBI3096591.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9968..10282
                     /gene="priB"
                     /locus_tag="K0W65_003465"
     CDS             9968..10282
                     /gene="priB"
                     /locus_tag="K0W65_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBI3096592.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10287..10514
                     /gene="rpsR"
                     /locus_tag="K0W65_003466"
     CDS             10287..10514
                     /gene="rpsR"
                     /locus_tag="K0W65_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBI3096593.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10556..11005
                     /gene="rplI"
                     /locus_tag="K0W65_003467"
     CDS             10556..11005
                     /gene="rplI"
                     /locus_tag="K0W65_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBI3096594.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11076..11639)
                     /locus_tag="K0W65_003468"
                     /pseudo
     CDS             complement(11076..11639)
                     /locus_tag="K0W65_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2768 a         2873 c         3086 g         2958 t
ORIGIN      
        1 ttccggcaat attatgtgcc tggatgtgtt gcgcgttctc aataagcaag cgggcgtata
       61 cgacttattg tcggaagcat ttgtggaagt gaaagggcag gatcgctatt ttgatcgcgc
      121 ggttcgtcgt ttacagcagc agctgcgtaa gccagctgaa gaactggggc gagagattac
      181 tcatcagcta ttcctgctgg gctgcggtgc gcaaatgttg aaatatgctt ctccgccaat
      241 ggcgcaggcg tggtgtcagg tgatgttaga tacgcgcggc ggcgtacggt tgtcagagca
      301 gatccagaat gatttattgc tgcgggcgac ggggggagtg tgtgtgtaag cgtatacgac
      361 tgatgcgacg ctggtttcga ttaactaaat gaaatatgtg aaaattgtag gccggacaag
      421 gcgctcgcgc cgcatccggc attgttcatt tgctgtagat gacactcatg catacagtat
      481 cgcctgtacg cgccagttat caggctgctg gtcttcgtac atggtaataa tgcggtacca
      541 tgacgcacct tgttcatcgg cctttaacgc cacgatacgt tccacgtcct gcggatcccc
      601 tgaaatattg ctaactgaga tctgacctat ttcattcagg cccgtcacgc agtcagcact
      661 ggcgaattct gcagactgtg ccgtggcgct cgcaggataa gtcactaatt gcagcgaggg
      721 tgaggcaagt aattgtttca tcgtcagctc catttttcgt ttccccacat gttgcagggc
      781 attccatcgc ggtaataaac gcccaagctt ccagccggta atttattgtg cacaggtaaa
      841 cgcataggga cgcaaagcaa tgtaaagctg actatgtaag ttgtctgatt tatttacggt
      901 acaaaatggc ttgtgaatac cactgtcctg tcacgatggt ttcgtccacc atgacgacaa
      961 cgtaataatc agcttttgca gcgacagctt ttgctctgat ttctgctaat gcgtcatccg
     1021 gagaaccccg aaccatcgtg cttacgctac ctattcgctg taacccttgc gtctggtcgc
     1081 ggcgaatctc ttgcggatgg tccgttactg gcggtgctgg ctgtggcgta ccttgcagtg
     1141 cgctacaggc acttaacatc aacaccaata ataaactggc aaaccggtaa ataacgctat
     1201 tacgtttcct gctaaccata gtgtagtgcc ttattaattt aactttgggg gaatgttccc
     1261 gaattgttac ctggtacgcg attttcgaat gaaaacgtga cgaaagcgta acccacatct
     1321 caacattatg aactaatacc gccagcagaa acttgtgcta aggatttctt catgtcatac
     1381 cagacaaaaa ggagagacag atgattgaaa tagaatcacg cgagctggca gatattcccg
     1441 ttcttcatgc ttatcctgtc gggcaaaaag ataccccgtt accgtgcgta attttttatc
     1501 acggctttac ttcatccagc ctggtgtata gctattttgc cgttgcgctg gcgcaggctg
     1561 gtttgcgggt gatcatgccg gatgcgcccg atcacggtag ccgttttagt ggcgacgaag
     1621 cgcggcggtt aaatcaattc tggcaaatct tgctacaaag tatgcaggaa ttcactactt
     1681 tacgtgcggc aatagccgaa gaaaactggc tgcttgatga ccgtctggca gtcggtggcg
     1741 cgtcgatggg cgcgatgacg gcactgggaa ttaccgctcg ccaccccacg gtgaaatgta
     1801 ccgccagcat gatgggatcg ggctatttta catcgctcgc ccgttcactg tttccaccgc
     1861 tgatacctga aacggcagca cagcagaatg aattcaataa aattgtcgcg ccgctggcag
     1921 agtgggaagc gacaaaccac ctggaacaac ttggggacag acctctactg ctgtggcatg
     1981 gcctcgacga tgacgttgtg cctgccgacg aatcactacg tttgcagcag gccttaagcg
     2041 agacgggacg ggataaactg ctaacctgtt catggcagcc aggcgtgcgt caccgcatta
     2101 cgcctgaggc gttagatgct gccgtgacat ttttccgcca gcatctttaa acacgcagaa
     2161 tgctgacccc ttgcgcttcc agttgttgca ggatttccgg gttagcattt ttgccggtga
     2221 tcagcatatc gatttgatcg gcacggctaa aaagcattcc cgcgcgttcg ccaatcttac
     2281 tgctatcaac cagtaccacc agtttcccta ccacgctcag catcttctgc tctgccattg
     2341 ctgtcagcat atcggtttta tacaaccctt ctgcggtcag cccttttccg ctggtaaaca
     2401 tccagtgccc ggcatagaga ctgttttcgc tgccctgcgg gcttaaagtg atggactgac
     2461 ttttgttgta ctgtccaccc ataatgatca cgctgtcatg ttcttgatcg atcaggtaat
     2521 ttgccagcgg tagataatta gtgatgattt gcactggctt gccacacatt tcccgcccaa
     2581 gcagaaacgc ggtggagccg cagttgatga ctacgctttc gcccggatta accagctgcg
     2641 aggccgcttt agcaatacgt actttttcat cgtgattctg cgcctgatgc agattcatcg
     2701 gcgtccagcg cgggcgctgt tgggtaatag cttctgcgcc attgcgcacc tttttcagtt
     2761 tgccgctttc gccaagttta ttgatatcgc gtcgcgcagt ggcaggcgaa attcccagac
     2821 gctcaacgac tttctcaacg gtcacaaagc ccaattgtgc gagcatttcc aggaggattt
     2881 gatgtctttg tgcttcagtc atgagctatt ccgataaaat ttgatttttt tagatgatat
     2941 ttgaaatagc caggaaatac tacgccggat agcgcgaact tctccacgct atccggtata
     3001 aacgacagat tacaggaatg acttgaacgg cagatccggt tcaatagtga agcaatcatc
     3061 gaaaccgcgc ggatagtggt actcgaagtt gtctttatcc agcggccagg taaatttgcc
     3121 gccaacctgc cagataaacg gcttgaagcc atacttcaga cgatcttttt tgatctccca
     3181 cagcacgcgg atctcttgcg gatcggcctg gaagtttgac cagatgtcgt ggtggaacgg
     3241 gataaccact ttcgcattca gggcttcacc catacgcagc atatcggcgc tggtcatttt
     3301 gtcggtaata ccgcgcgggt tctcaccata cgatcctaac gccacatcga tctgatgttc
     3361 gttaccgtgc ttcgcataat agttagagta gtgggaatcg ccactgtgat atagggaacc
     3421 gccaggggtt ttgaacaggt agtttactgc gcgatcgtcc atgccatctg gcagtacgcc
     3481 agccgctttt tgatcggcag gcagggtgat cagtgcagta cggtcgaaag cgtcaagcgc
     3541 atgaatttca atgtctttca cttttacaac atcgcccggt ttgaccacga tgcaacgctc
     3601 tttcggtacg ccccagccaa tccacaaatc aacgcaggtt ttcggtccaa tgaatggtac
     3661 gtcatcagca cagttctgca tcacggcagc agcgacgtta acgtcgatat gatcgttgtg
     3721 atcgtgagtc gccagtaccg cgtcgatctg gcgaatcgca aacggatcaa gaacaaacgg
     3781 ggtggtgcgt aggtttggct gcagtttttt cacgccagcc atgcgctgca tctggtgacc
     3841 ctgtttcatt aacgggttac cgtgactttg tttgccagtg ccgcaccaga aatcaacgca
     3901 aacgttggtg ccaccttctg atttcaacca gatcccggtg cagcctagcc accacatcgc
     3961 aaatgtgcca ggggcgacct gctcttgttc aatttcttca ttcagccagc taccccactc
     4021 cgggaaagtg ctcaggatcc aggattctcg ggtgatactt ttcactttac tcatcgccat
     4081 ttaccttcat gatagttcaa ttcgaatcaa catgtgattg gttttgatta atcctgacac
     4141 tattccttca ggaaggtaat gaccattttt tgactttttg ccagggaagt tgttgttgat
     4201 tttgagtggg gaaagattta atggaatgtg tagttaaatt aactaaatga atttaaataa
     4261 ataattgttt cgttgtgtga atcccactct atccatgtgg aattatttgc gggtcgcgtc
     4321 acatttaatc ataaataatc ttgttgtgat tacttttgaa aattagagtg agtgcacaac
     4381 attccgggtg tgcggaatac ccggttacct cttcttcagg agatcgttat ggagatcctc
     4441 tacaacatct ttaccgtgtt ttttaaccag gtcatgacca atgccccgtt gttgctgggt
     4501 attgtgacct gtctgggcta catcctactg cgcaaaagtg tcagcgttat tattaaaggc
     4561 acgattaaaa ccataattgg tttcatgttg ttgcaggcag ggtccggcat cctcaccagc
     4621 accttcaaac cggtggtggc gaaaatgtcc gaagtctacg gcattaacgg cgcaatttcc
     4681 gatacttacg cgtcgatgat ggcaaccatc gaccgcatgg gcgatgccta tagctgggta
     4741 ggttacgcgg tgttgttagc gctggcgctg aacatctgtt acgtgctgct gcgtcgcatt
     4801 accggtattc gtaccatcat gctgactggt catatcatgt tccagcaggc ggggctgatt
     4861 gccgttacgt tgtttatttt cggctactcc atgtggacaa ccattatctg cacggcgatt
     4921 ctggtttcgc tctactgggg cattacctcc aacatgatgt acaagccgac tcaggaagtg
     4981 acggacggct gcggtttctc catcggtcac cagcaacagt ttgcatcatt aattgcctat
     5041 aaagttgcgc cgttcctcgg caaaaaagag gaaagcgttg aagacctcaa actgccaggc
     5101 tggctgaata ttttccacga caacatcgtc tccacggcga ttgtcatgac catcttcttt
     5161 ggtgccattc tgctctcctt cggtatcgac accgtgcagg cgatggcagg caaagtgcac
     5221 tggacggtct atatcctgca aaccggtttt tccttcgcgg tggcgatctt catcatcact
     5281 cagggtgtgc gcatgtttgt ggcggaactc tctgaagcgt ttaacggtat ctcccagcgc
     5341 ctgatccctg gtgcggttct ggcgattgac tgtgcggcta tttatagctt cgctccgaac
     5401 gccgtggtct ggggctttat gtggggcacc atcggtcagc tgattgcggt tggcatcctg
     5461 gtcgcctgcg gttcctcgat cctgattatc ccaggcttta tcccgatgtt cttctccaac
     5521 gccaccatcg gcgtgttcgc taaccacttc ggcggctggc gtgcggcgct gaagatttgt
     5581 ctggtgatgg ggatgatcga aatctttggt tgcgtctggg tggtgaaact taccggtatg
     5641 agtgcctgga tggggatggc ggactggtcg attctggcac cgccgatgat gcagggcttc
     5701 ttctccatcg gtatcgcctt tatggccgtc atcattgtaa ttgcactggc ttatatgttc
     5761 ttcgctggcc gcgcactgcg cgcagaagaa gatgcagaaa aacaattggc agaacagtct
     5821 gcttaataag gagttttgat tatgaccgta cgtattctgg ctgtgtgtgg caacggacaa
     5881 ggcagttcca tgatcatgaa gatgaaagtg gaccagtttt taacccaatc aaacattgac
     5941 cacacggtaa atagctgcgc ggttggcgaa tacaaaagcg agttgagcgg cgcggatatc
     6001 atcatcgctt ctacgcacat tgcgggcgaa atcaccgtga ccggcaacaa atacgtggtt
     6061 ggcgtgcgca acatgctctc tcctgccgac tttggcccga aactgctgga agtgatcaaa
     6121 gcgcatttcc cgcaggatgt gaagtaagga cgcgccatga aattacatga ttcgctggcg
     6181 gagaataaat ccatccgcct gcaggctgaa gctgagacat ggcaggacgc agtaaaaatc
     6241 ggcgttgacc tgctggtggc ggcggatgtt gtcgagccgc gttactacca ggcgattctg
     6301 gatgcggttg aacagcacgg tccgtacttt gtgctcgcac caggcctggc aatgccgcac
     6361 ggtcgcccgg aagagggcgt taagaaaacc ggttttgcgc tggtgacgct gaaaaagccg
     6421 ctggagttca accacgaaga taacgacccg gtggatatcc tcatcaccat ggcggcggtc
     6481 gatgccaaca cgcaccagga agtgggcatc atgcagatcg tcaacctgtt tgaagatgaa
     6541 gaaaatttcg accgtttacg tgcctgccgt accgagcagg aagtactgga tctcattgac
     6601 cgcaccaacg cggcagctta agaaggaatt gaagatgtca ttaccgatgt tgcaagtcgc
     6661 gctggacaac cagactatgg atagcgccta cgaaaccact cgcctgattg ccgaagaagt
     6721 cgacattatc gaagtgggta ccattctgtg cgtgggcgaa ggcgtgcgtg cggttcgcga
     6781 tctgaaagcg ctctacccgc acaaaatcgt gctggcagac gccaaaattg ccgatgcggg
     6841 caaaattctt tctcgtatgt gcttcgaagc caacgctgac tgggtgaccg tgatttgttg
     6901 tgcggatatc aacaccgcca aaggtgcgct ggacgtagca aaagagttta acggcgacgt
     6961 gcagatcgaa ctgaccggtt actggacctg ggaacaggcg caacagtggc gcgacgcagg
     7021 tattcagcag gtggtttatc accgcagccg tgacgcgcag gccgctggcg tggcgtgggg
     7081 tgaagccgat atcactgcga tcaaacgtct ttccgatatg ggcttcaaag tcaccgtcac
     7141 cggtggcctg gcgctggaag atctgccgct gttcaaaggc attccaattc atgtgtttat
     7201 cgccggtcgc agcatccgtg atgccgcttc tccggttgaa gccgcgcgtc agttcaaacg
     7261 ttccatcgcc gaactgtggg gctaaggagc ggatatgttg tccaaacaaa tcccgcttgg
     7321 catctatgaa aaagcgctcc ccgccgggga gtgctggctg gaacgcctgc gactggcaaa
     7381 aacgttgggc ttcgattttg tcgagatgtc ggtagatgaa actaacgagc gcctgtcgcg
     7441 cctcgactgg agccgcgaac agcgtctggc gctggtgaat gctattgttg aaaccggcgt
     7501 gcgtgtgccg tccatgtgcc tttctgccca tcgtcgtttc ccgttgggca gcgaagatga
     7561 cgcggtgcgg gcgcaggggc tggagattat gcgtaaagcc atccagttcg cccaggacgt
     7621 tggcattcgc gtgatccagc tggcgggcta tgacgtttac tatcaggaag ccaataacga
     7681 aacgcgtcgt cgtttccgtg acggcctgaa agagagcgtt gagatggcga gccgcgcgca
     7741 ggtgacgctg gcgatggaga tcatggatta tccgttgatg aactccatca gcaaggcgct
     7801 gggttacgcg cactatctca acaacccgtg gttccagctc tacccggata tcggcaacct
     7861 gtcggcgtgg gacaacgatg tgcagatgga gttgcaggcc ggaatcgggc atatcgtcgc
     7921 ggtgcatgtg aaagacacca aaccgggcgt cttcaaaaac gtgccatttg gcgaaggtgt
     7981 ggtggatttc gaacgttgct tcgaaacgct caaacagagt ggctattgcg ggccgtacct
     8041 gattgagatg tggagcgaaa cggcggaaga cccggcggca gaagtggtga aagcgtgtga
     8101 ttgggtgaaa gcgcgcatgg ccaaagcagg catggtggag gcggcataat gcaaaagcta
     8161 aaacagcaag tgtttgaagc caacatggat ctgccgcgct atgggctggt gacctttact
     8221 tggggcaacg tcagcgctat cgaccgcgaa cgcgggctgg tggtgatcaa gcccagcggc
     8281 gttgcctacg aaaccatgaa agcagacgat atggtggtgg tcgatatgag cggcaaggtg
     8341 gtggaagggg agtatcgccc atcttccgac actgcgacgc atctcgaact ctaccgtcgt
     8401 tacccgtcgc ttggcggcat tgtccatacc cactccactc atgccaccgc atgggcgcag
     8461 gcggggctgg cgatcccggc attaggcacc acgcacgctg actacttctt tggtgatatc
     8521 ccgtgtacgc gtggattaag cgaagaagag gtgcagggtg agtatgaact gaacactggc
     8581 aaagtgatta tcgaaacgct gggcaacgcc gagccgctgc atacgccggg aattgtggtg
     8641 tatcagcacg gaccgttcgc ctgggggaaa gatgcccacg acgcggtgca taacgcggta
     8701 gtgatggaag aagtggcgaa aatggcgtgg attgcgcgca gtattaatcc acaactcaat
     8761 catatcgaca gctacctgat gaataaacac ttcatgcgta agcacggtcc taacgcttat
     8821 tacgggcaga agtaaaacac gcgctgcgga aatttccttc ctcgggagat aactggtcta
     8881 attccgcagc cgtttttcaa aaaaagcccc ctgcgaaggg ggcaaagcaa actatggcaa
     8941 tgtttcgttg gttatacctg gtgctagcga taaatatccg cgctggcgtg catattgccg
     9001 ttactccccg gttcccgcat cagaattacg tggtagtacg ttgcgccttg cgcatctgtt
     9061 tcttcattta atgcctgacg tgcttcgctt tcagtggcga aattatgatt gatataaatc
     9121 acgcctaagc tttgcacatc gtccatattt ctggcctggt ggttattaat ttcaatggct
     9181 gcccatgtat ttgcacttag caaaagcaca gccagaaggg ctaaaactcg actgaacata
     9241 gatacctcct cgacggctga ctttgtgtgc tctcctctgt gatgatcttc tgatttaatt
     9301 ttaatcaatg ataaagaagt tgatggtgac catttctgat gcagttgttc aaaaaaacac
     9361 tgtgacggaa tgtgatgaac ttcaaatcag cggtttagag gttatttgcg aaaggggaga
     9421 tttatttctg ctccgccctt gagtttagcg gggtatgcca gtactataac gcgtcatttt
     9481 tcagccgacc tttaacacgt tccttgcctc cccgggattc ggctgaccca gacaggaggc
     9541 tgaataatcc gtaaggagca attcgatgcg tcattacgaa atcgttttta tggtccatcc
     9601 tgaccagagc gaacaggttc cgggcatgat cgagcgctac actgctgcca tcactggtgc
     9661 agaaggcaag atccaccgtc tggaagactg gggccgccgt cagctggctt acccgatcaa
     9721 caaactgcac aaagcacact acgttctgat gaacgttgaa gctccgcagg aagtgatcga
     9781 tgagctggaa actaccttcc gcttcaacga tgccgttatc cgcagcatgg ttatgcgtac
     9841 caagcacgct gttaccgaag catctccgat ggttaaagcg aaagacgagc gccgtgagcg
     9901 tcgcgatgat ttcgcaaacg aaaccgctga tgatgctgaa gctggggatt ctgaagagta
     9961 atttctgatg accaaccgtc tggtgttgtc cggcaccgtg tgcaggactc cccttcgaaa
    10021 ggtcagtcca tcaggaattc ctcactgcca gttcgtgctt gagcatcgtt ctgtgcagga
    10081 ggaagccggc tttcaccggc aggcatggtg tcaaatgccc gttattgtta gcggacacga
    10141 aaaccaggcc attactcaca gtataacggt cggcagtcgc ataaccgttc aggggttcat
    10201 ttcatgccac aaggcaaaga acggactgag caaaatggtt ttgcatgccg agcagattga
    10261 attgatagat tctggagact agccatatgg cacgttattt ccgtcgtcgc aagttctgcc
    10321 gtttcaccgc ggaaggcgtt caagagatcg actataaaga tatcgctacg ctgaaaaact
    10381 acatcaccga aagcggtaag attgtcccaa gccgtatcac cggtacccgt gcaaaatacc
    10441 agcgtcagct ggctcgcgct atcaaacgcg ctcgctacct gtccctgctg ccgtacactg
    10501 atcgccatca gtaatcggtc acggtccatt aatacgactt tgagaggata aggtaatgca
    10561 agttattctg cttgataaag tagcaaacct gggtagcctg ggtgatcagg taaacgttaa
    10621 agcgggctat gctcgtaact tcctggtacc gcagggtaaa gctgttccag ctaccaagaa
    10681 aaacattgaa ttcttcgaag cacgtcgcgc tgaactggaa gctaaactgg ctgaagttct
    10741 ggcagctgct aatgctcgcg ctgagaaaat caatgcactg gaaactgtta ccatcgcgtc
    10801 taaagctggc gacgaaggta aactgttcgg ttccatcggt actcgcgaca tcgctgacgc
    10861 tgtaactgca gctggcgttg aagtggctaa gagcgaagtt cgtctgccga acggcgttct
    10921 gcgtaccact ggcgaacacg aagtgagctt ccaggttcac agcgaagtat tcgcgaaagt
    10981 gatcgtaaac gtagtagctg aataattcgt tattcaacga gacgtaaaaa cgccgaccat
    11041 tggtcggcgt tttgctttct atttttcgtc aggcattaat ttcgcaagta gatccattca
    11101 ccattctctt gtgtccataa gtcaggatgg tttgctggcg ttagatgcat acgatcactc
    11161 cttatgtctc ggtcctgcgg cactcgctcg aaacctaaac ccgagtagat tgcggctgaa
    11221 tttgataagg aacgtagacc aattttcaaa tcatggggta aatcagaata cgcgtttagc
    11281 aatgcagcaa tgatgaatcc gagggcatta gaaacgcctg taacagtgtg aagttggtcg
    11341 atataaagat cgattgttgg tagttcagat gagcagcaat taggcatttg ggcaggaagg
    11401 gttacaacgt atcctatcgt ttggccggta tagtctggag tactataacc aggtcccaaa
    11461 acaacctcgt gtattgtgta tttactgttg ggttcgaagg attcgacatc ttgcaataaa
    11521 tatttcagtt ctgcggttaa cctcgcaccc gcgtaagctt tattgtattt ctgggcgtga
    11581 atattatcta atgtagcttc atggttccat ccaaaagaat tatcaccatt cattgttact
    11641 gaatcgccac gggtttaaca gacacctcag agtcatttaa gatgg
//
LOCUS       DAETQL010000111        11590 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.111, whole genome shotgun sequence.
ACCESSION   DAETQL010000111 DAETQL010000000
VERSION     DAETQL010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11590
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.111"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(282..357)
                     /locus_tag="K0W65_003469"
     tRNA            complement(282..357)
                     /locus_tag="K0W65_003469"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Ile,seq:cat)
     gene            916..3168
                     /locus_tag="K0W65_003470"
     CDS             916..3168
                     /locus_tag="K0W65_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096595.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3504..4436
                     /gene="csiD"
                     /locus_tag="K0W65_003471"
     CDS             3504..4436
                     /gene="csiD"
                     /locus_tag="K0W65_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HBI3096596.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4456..5724
                     /gene="lhgO"
                     /locus_tag="K0W65_003472"
     CDS             4456..5724
                     /gene="lhgO"
                     /locus_tag="K0W65_003472"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HBI3096597.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5747..7195
                     /gene="gabD"
                     /locus_tag="K0W65_003473"
                     /pseudo
     CDS             5747..7195
                     /gene="gabD"
                     /locus_tag="K0W65_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7203..8489
                     /gene="gabT"
                     /locus_tag="K0W65_003474"
                     /pseudo
     CDS             7203..8489
                     /gene="gabT"
                     /locus_tag="K0W65_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8727..10127
                     /gene="gabP"
                     /locus_tag="K0W65_003475"
     CDS             8727..10127
                     /gene="gabP"
                     /locus_tag="K0W65_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HBI3096598.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10148..10810
                     /gene="csiR"
                     /locus_tag="K0W65_003476"
     CDS             10148..10810
                     /gene="csiR"
                     /locus_tag="K0W65_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HBI3096599.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10811..11260)
                     /gene="kbp"
                     /locus_tag="K0W65_003477"
     CDS             complement(10811..11260)
                     /gene="kbp"
                     /locus_tag="K0W65_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBI3096600.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11344..11502)
                     /locus_tag="K0W65_003478"
     CDS             complement(11344..11502)
                     /locus_tag="K0W65_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBI3096601.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2981 a         2847 c         2889 g         2873 t
ORIGIN      
        1 tccggaacaa tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg
       61 atcaatttgc ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga
      121 tgaataaata cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca
      181 cccaccgcac ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac
      241 cccttgtacc cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact
      301 tgaaccagcg accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta
      361 agtatgcaat aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat
      421 aacagatttt tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg
      481 acaccgcctc cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc
      541 actatctata gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat
      601 aactttatta cattagctga agagttttcg catcattatg atatctgtta cttttcactc
      661 cataaaaata aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt
      721 agtttcgtat gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac
      781 agatttaaac attatccttt tccatataga tattggataa aaataggaca tctgtttgca
      841 attactttca caacaattaa acatttttat gttttcgtat acatcatatt actctaccat
      901 tagaggaacg ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac
      961 cccacattgt tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta
     1021 gaatatctaa actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta
     1081 aaagacagag aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa
     1141 aacgattata aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca
     1201 cagagtagca ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga
     1261 gatacaatgg ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat
     1321 ttcactcaca ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt
     1381 aacattcctg actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc
     1441 ggacagcaag aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag
     1501 cgtaaatctc ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct
     1561 ggcgaagcga ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct
     1621 gagtttgcac gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc
     1681 gacggaccag aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa
     1741 gatctttgta ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa
     1801 gatgctaaaa aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc
     1861 gggggaagtg gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt
     1921 aaagatatag aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt
     1981 aaagatccag taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca
     2041 aaagacttcg aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca
     2101 ttaaaagaaa agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt
     2161 atgttgcaaa atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat
     2221 gatgcagcaa aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa
     2281 aattataatg agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc
     2341 gttatgaatt acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg
     2401 ctgctttatg aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata
     2461 aaagcctttt cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc
     2521 tcaagcatcc catcagaaag acggatattg attaacatta accacgattt tcctaacaat
     2581 ggcaatcttt tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca
     2641 tatatggccg ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa
     2701 tcaacgactg aaattaaaaa ttatgatggg cccacgcgtg tcggtggtga ggcgtggctt
     2761 aataaaggtt gctctaccta tcagcagctc tacaatgaat tccacgcatt atatatagat
     2821 aaagcaggaa tatggagcgc atttgagggt gtatttgcaa ctaaaaacgt tctggccttt
     2881 agtcgtgggg attctgtgaa cattaatcac tctcctcatg atggactagt tataataaat
     2941 aaaggaaacg aagaagttga aggtacctgg cctaacaaat tgcaacctgg aatatataaa
     3001 aacatgggga gtaatagcgt taacattatt attaataata cccgaaaaat tatcccccct
     3061 tgtaaagtat ttacgcttag aggcggatct ctaaatatca atattcctag gcgtagcgct
     3121 cttcttttag ggaaaacagg agaaccgccg aactatctct atttataatt taatgttata
     3181 tctgccccga taaaacgggg cagataatat gtttagttta ctaacggtca ttttgtagtg
     3241 aagccattta ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa
     3301 cctgttgctt tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt
     3361 tactaatttg ttgcttttga tcacaataag aaagcaatat gtcgcttttg tgcgcatttt
     3421 tcagaaatgt agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac
     3481 atctcataag aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg
     3541 attcaggcca ggactatagc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc
     3601 tggaactcac cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc
     3661 ccgtgcaggc gctggagtac aaatcgtttc tgcgttttcg ggtaggcaaa attcttgacg
     3721 atctgtgtgc gaatcagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag
     3781 gtgcgctgtt gatcaatgcg gtaggtatcg atgatgtggc gcaggcagat gagatggtga
     3841 agctggcaac ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc
     3901 agtattacgc gcgtttcgtg gtgaaaaatg tcgataactc agacagctat ctgcgtcagc
     3961 cgcaccgcgt gatggaactg cacaacgacg gcacgtatgt cgaagagatc accgattacg
     4021 tgctgatgat gaaaatcgat gactgggaac atctggacca ctatttccgc catccactgg
     4081 cgcgtcgccc gatgcgcttt gccgcgccgc cgagcaaaaa cgtcagcaaa gatgttttcc
     4141 atccagtgtt cgacgttgat caacagggtc gcccggtgat gcgctatatc gaccagttcg
     4201 tccagccaaa agattttgaa gaaggcgtgt ggttgagcga gctttccgat gccattgaaa
     4261 ccagcaaagg cattctttct gtacccgttc ccgttggcaa attcctgttg ataaacaacc
     4321 tgttctggct gcacggtcgc gaccgcttta ctccgcaccc ggatctgcgc cgtgaactga
     4381 tgcgtcagcg tggctatttc gcttacgcca ctcaccacta ccagacgcat cagtaagcgc
     4441 aaaggaattg agcggatgta tgattttgtg attattggcg gcggcatcat cggcatgtcg
     4501 accgccatgc aactgattga tgtttatccg gatgcccgca ttgcgttgct ggaaaaagag
     4561 tccggcccgg cctgtcacca gacgggccac aacagcggcg tgatccatgc cggggtctat
     4621 tacacgcccg gcagcctgaa ggcgcagttt tgcctggcgg ggaaccgtgc caccaaagcc
     4681 ttttgcgatc aaaacggcat tcgctatgac aactgcggca agatgctggt cgccacgtct
     4741 cagctcgaaa tggaacggat gcgcgcctta tgggaacgca cagcggcgaa cggtatcgag
     4801 cgcgagtgga taaacgccgt ggaactgcgc gagcgcgaac cgaatatcac cgggcttggt
     4861 ggcatttttg tgccgtccag cggcattgtc agctaccgcg aagtgacggc ggcaatggca
     4921 aaaatcttcc aggccagagg cggcgagatt atttataacg ccgaagtcag cgccctcagt
     4981 gagcataaaa acggcgtggt gatacgcacc cgtcagggcg gtgaatatga agcatcgacg
     5041 ctgattagct gttccgggct gatggctgac cggctggtga aaatgctcgg cctcgaaccg
     5101 ggctttatta tctgcccgtt ccgtggcgag tatttccgcc ttgcgccgga gcataaccag
     5161 attgttaacc acctgattta ccccattccc gaccccgcga tgccattttt gggcgtgcat
     5221 ctcacccgca tgatcgacgg cagcgtgacc gtcgggccaa acgcggtgct ggctttcaaa
     5281 cgcgaaggct atcgcaagcg cgacttctca tttagtgaca cgctggagat tttgagctcg
     5341 tcggggattc gccgggtgct gcaaaaccat ctacgctcag gactgggcga gatgaaaaac
     5401 tcgctgtgca aaagcggcta tctgcggctg gtgcaaaagt attgtccccg gctttcgtta
     5461 agcgatctcc agccctggcc cgccggtgtg cgggcgcagg cagtatcgcc ggacggcaag
     5521 ctgattgacg attttctgtt tgtcaccacc ccacgcacga tccacacctg caatgcgccc
     5581 tccccggcag cgacatcagc aattcctatt ggtgcgcata ttgtcagcaa ggtacaaacg
     5641 ctgttggcaa gccagagtaa ccccggacgc acgctgcgag cggcacgtag tgtggatgcc
     5701 ttacacgccg catttaatca ataacctttg aaaacaggac gtaacaatga aacttaacga
     5761 cagtaactta ttccgccagc aggcgttgat taacggggaa tggctggacg ccaacaatgg
     5821 tgaagtcatc gacgtcacca atccggcgaa cggcgacaag ctgggtagcg tgccgaaaat
     5881 gggcgcagat gaaacccgcg ccgctatcga cgccgccaac cgcgccctgc ccgcctggcg
     5941 tgcgctcacc gccaaagaac gcgccaccat tctgcgcaac tggttcaatt tgatgatgca
     6001 gcatcaggac gatttagcgc gcctgatgac cctcgaacag ggtaaaccgc tggctgaagc
     6061 gaaaggcgaa atcagctacg ccgcctcctt cattgagtgg tttgctgaag aaggcaaacg
     6121 tatttatggc gacaccattc ctggtcatca ggccgataag cgcctgattg ttatcaagca
     6181 gccgattggc gtcaccgccg ccatcacgcc gtggaacttt ccggcggcga tgattacccg
     6241 taaagccggt ccggcgctgg cggcaggctg caccatggtg ctgaagcccg ccagtcagac
     6301 gccgttctct gcgctggcgc tggcggaact ggcgattcgc gcgggcattc cggctggcgt
     6361 gtttaacgtg gtcaccggtt cggcaggcgc ggtcggtaat gaactgacca gcaacccgct
     6421 ggtacgcaaa ctgtcgttta ccggttagac cgagattggc cgccagttaa tggaacagtg
     6481 cgcgaaagac atcaaaaaag tgtcgctgga gctgggcggc aacgcgccgt ttatcgtctt
     6541 tgacgatgcc gatctcgata aagccgtgga aggcgcgctg gcctcgaaat tccgcaacgc
     6601 cgggcaaacc tgcgtctgcg ccaaccgttt atacgtgcag gacggcgtgt atgaccgctt
     6661 tgccgaaaaa ttacagcagg cggggagcaa actgcacatc ggcgacgggc tggagaaagg
     6721 cgtcaccatc gggccgctga tcgatgaaaa agcggtagca aaagtggaag agcatattgc
     6781 cgatgcgctg gagaaaggtg cgcgtgtggt ttgcggcggt aaagcgcacg aacgcggcgg
     6841 caacttcttc cagccaacca ttctggtgga cgttccggcc aacgccaaag tgtcgaaaga
     6901 agagacgttc ggccccctcg ccccactgtt ccgttttaaa gatgaagccg atgtgattgc
     6961 acaagccaat gacaccgagt ttggtcttgc cgcctatttc tacgcccgtg atttaagccg
     7021 cgtcttccgc gtcggcgagg cgctggagta cggcatcgtc ggcatcaata ccggcattat
     7081 ttccaatgaa gtggccccat tcggcggcat caaagcctcg ggtctgggtc gtgaaggttc
     7141 gaagtatggc atcgaagatt acttagaaat caaatatatg tgcatcggtc tttaactgga
     7201 gaatgcgaag gaacagcaat aaagagttaa tgcagcgccg cagtcaggcg attccccgtg
     7261 gcgttgggca aattcacccg attttcgctg accgcgcgga aaactgccgg gtgtgggacg
     7321 tttaaggccg tgagtatctt gatttcgcgg gcgggattgc ggtgctcaat accgggcacc
     7381 tgcatccgaa ggtggtggcc gcggtggaag cgcagttgaa aaaactgtcg cacacctgct
     7441 tccaggtgct ggcttacgag ccgtatctgg agctgtgcga gattatgaat cagaaggtgc
     7501 cgggcgattt cgccaagaaa acgctgctgg ttacgaccgg ttccgaagcg gtggaaaacg
     7561 cggtaaaaat cgcccgcgcc gccaccaaac gtagcggcac catcgctttt agcggcgagt
     7621 atcacgggcg cacgcattac acgctggcgc tgaccggcaa ggtgaatccg tactctgcgg
     7681 gcatggggct gatgccgggt catgtttatc gcgcgcttta tccttgcccg ctgcacggca
     7741 taagcgagga tgacgctatc gccagcatcc accggatctt caaaaatgat gccgcgccgg
     7801 aagatatcgc cgccatcatg attgagccgg ttcagggcga aggcggtttc tacgccgcgt
     7861 cgccagcctt tatgcagcgt ttacgcgctc tgtgtgacga gcacgggatc atgctgattg
     7921 ccgatgaagt gcagagcggc gcggggcgta ccggcacgct gtttgcgatg gagcagatgg
     7981 gcgtggcgcc ggatcttacc acctttgcga aatcgatcgc aggcggtttc ccgctggcgg
     8041 gcgtcaccgg gcgcgcggaa gtgatggacg ctgttgcacc gggcggtctg ggcggcacct
     8101 atgccggtaa cccgattgcc tgcgtggctg cgctggaagt gttgaaggtg ttcgagcagg
     8161 aaaatctgct gcaaaaagcc aacgatctgg ggcagaagct aaaagacgga ttgttggcga
     8221 tagccgaaaa acacccggag atcggcgacg tacgcgggct gggatcgatg attgccatcg
     8281 agctgtttga agatggcgat cacagcaaac cggacgcaaa actcaccgcc gagatcgtgg
     8341 cacgcgcccg cgataagggc ctgattcttc tctcctgcgg cccgtattac aacgtgctgc
     8401 gcatccttgt accactcacc attgaagacg ctcagatccg tcagggtctg gagattatcg
     8461 gtcagtgttt tgatgaggcg aagcagtagc accgtcccca tgcaggaggc gacgctgcgc
     8521 gtcttgtccg gcctacgaga atccaagtcg gataaggcgt ttacgccgca tccgacaatc
     8581 tgtacgtgaa caggaataaa tctatgtcgg ccggataagg cggagccgct ctccggcaaa
     8641 aagaatcaat aacaattaaa cgcgtgaccc gacgcgggaa atgtcggggc gctctcccaa
     8701 gtgacacact ttcgagagga ttcaggatgg ggcaatcatc gcaaccacac gagttaggcg
     8761 gcgggctgaa atcacgccac gtcaccatgt tgtctattgc cggtgttatc ggcgcaagtc
     8821 tgtttgtcgg ttccagcgtc gccatcgccg aagcggaccc ggcggtatta ctggcctatc
     8881 tgttcgccgg attactggtg gttatgatta tgcggatgct ggcggaaatg gcagttgcca
     8941 cgcccgatac cggttcgttt tccacctatg ccgataaagc cattggccgc tgggcgggct
     9001 ataccatcgg ctggttgtac tggtggtttt gggtactggt tatcccactg gaagccaaca
     9061 tcgccgccat gatcctgcac tcgtgggttc caggcattcc catctggtta ttttccctcg
     9121 tcattaccct cgccttaact ggcagtaatt tattaagcgt taaaaactac ggcgaatttg
     9181 agttctggct ggcgctgtgc aaagtcatcg ctatcctggc ctttattttc cttggtgcag
     9241 tcgcaattag cggtttttac ccttatgccg aagtgagcgg gatctcaaga ttgtgggata
     9301 gcggcggctt tatgcccaac ggtttcggtg cggtattaag cgcgatgttg atcaccatgt
     9361 tctcgtttat gggcgcagaa attgtcacca ttgccgccgc ggaatccgac atgccggaaa
     9421 aacatattgt ccgcgccacc aactcggtta tctggcgtat ttctatcttc tatttgtgct
     9481 ctatttttgt cgtcgtggca ttaataccgt ggaatatgcc ggggctgaaa gccgttggat
     9541 cttatcgctc ggttctggaa ttgctcaata ttccccatgc gaaattaatc atggactgtg
     9601 tgatattact ttccgtaacc agttgcctga actcggccct gtataccgcc tcaaggatgc
     9661 tctactcctt aagccgtcgc ggtgatgctc ccgcggtaat gggtaaaatc aaccgcagta
     9721 aaaccccgta cgtggcggtg ttactctcca ccggcgcagc attcttaacg gtggtggtga
     9781 actattacgc gcctgcgaag gtatttaaat ttctgatcga cagctccggc gctatcgccc
     9841 tgctggttta tttagtcatc gccgtttcac agttgcggat gcgcaaaatt ctgcgagcag
     9901 aaggaagcga aattcgcttg cgaatgtggc ttgatccctg gctcacctgg ctggtcatcg
     9961 gctttattac ctttgtgttg gtagtgatgc tattccgtcc ggcgcaacag ttagaagtga
    10021 tctccaccgg cttattagcg atagggatta tctgtaccgt gccaattatg gctcgctgga
    10081 aaaagctggt attgtggcaa aaaacacccg ttcataatac gcgctgataa tgatcaggag
    10141 tcacaccatg accattacgt ctctggatgg ctatcgctgg ctgaagaacg atattattcg
    10201 cggtaatttt caaccggatg aaaaattacg gatgagttta ctgacatcgc gttatgcact
    10261 tggcgttggg ccgttgcagg aagcactttc tcaactggtg gcggaacggc tggtcacggt
    10321 ggtgaatcaa aaagggtatc gggtggcgtc tatgtcagag caggagctgc tcgatatttt
    10381 cgacgcccgc gccaatatgg aagcgatgct ggtcagtctg gcgattgccc gcggtggcga
    10441 tgaatgggag gcagacgttc tcgcaaaagc gcatctgctc agtaagctgg aagcctgtga
    10501 cgccagcgag aaaatgcttg atgagtggga tctgcgtcat caggcgtttc atacagcaat
    10561 tgtggcgggc tgtggttctc actatttgct gcaaatgcgt gaacggttgt ttgatctggc
    10621 ggcgcgttat cgatttatct ggctgcggcg aacggtgctt tcggtggaaa tgctggagga
    10681 taaacacgat cagcaccaga ccctgactgc ggcggtactg ccgcgagata cagcgcgcgc
    10741 cagtgagtta atgcgccagc atttactgac gccaattccc attatccagc aggcgatggc
    10801 tggcaattaa ctactcttcc ggaatacgca acacttgccc cggataaatt ttatccgggc
    10861 tttttagcat cggtttattc gcttcgaaga ttttattgta cagattagcg ttaccgtaga
    10921 cctgtttgga aatggcactc agagtgtcgc cagacttaac ggtataaaac tggctggcag
    10981 tggctggtgt cgccgttttc acctgatcat caacactggc aataccggaa atattcccca
    11041 ccgcaacaag gattttctcc ttcgcctcct gactcaggcc gtcaccagtg accgtcgctt
    11101 tgccgtcggc aatttgaatg ttcactttat cggcatcagg tataccggtt ttgctcagat
    11161 gctcctgcac cttcttcgcc tgatcgtctt tatcgtgctg acctgtaacc gcatcccaga
    11221 gtttttctcc ggcatcttta acaaaattga acagacccat agctacctca ttcgttaacg
    11281 gaaacactca gaaagtgtag cagaggaacg cggcgcgtta acgccgcggg ggtgagcaca
    11341 ggtttaatcg cgggtttgca cccagaacgc gtgaatcaaa ccggggatat acccaagcag
    11401 tgtcagcaga atattaataa tgaacgccca accgaaccct ttaccgagca gcacgccgat
    11461 cggcggcaga atgatggtga tgacgattct ccagaaaccc atatgtactc cctataagaa
    11521 aattactcat tgtttaaaaa gagattttat ctcttaagcg tagtaatttt agcggagcct
    11581 gccagtttca
//
LOCUS       DAETQL010000112        11462 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.112, whole genome shotgun sequence.
ACCESSION   DAETQL010000112 DAETQL010000000
VERSION     DAETQL010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11462
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.112"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="K0W65_003479"
     CDS             complement(<1..609)
                     /gene="hdhA"
                     /locus_tag="K0W65_003479"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HBI3096602.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(721..1749)
                     /gene="malI"
                     /locus_tag="K0W65_003480"
     CDS             complement(721..1749)
                     /gene="malI"
                     /locus_tag="K0W65_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HBI3096603.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1924..3516
                     /gene="malX"
                     /locus_tag="K0W65_003481"
     CDS             1924..3516
                     /gene="malX"
                     /locus_tag="K0W65_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBI3096604.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3526..4698
                     /gene="malY"
                     /locus_tag="K0W65_003482"
     CDS             3526..4698
                     /gene="malY"
                     /locus_tag="K0W65_003482"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBI3096605.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4802..5803
                     /gene="add"
                     /locus_tag="K0W65_003483"
     CDS             4802..5803
                     /gene="add"
                     /locus_tag="K0W65_003483"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBI3096606.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5839..6879)
                     /locus_tag="K0W65_003484"
     CDS             complement(5839..6879)
                     /locus_tag="K0W65_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBI3096607.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7122..7247
                     /gene="blr"
                     /locus_tag="K0W65_003485"
     CDS             7122..7247
                     /gene="blr"
                     /locus_tag="K0W65_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBI3096608.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7520..7735
                     /gene="ydgT"
                     /locus_tag="K0W65_003486"
     CDS             7520..7735
                     /gene="ydgT"
                     /locus_tag="K0W65_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBI3096609.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7821..8261
                     /locus_tag="K0W65_003487"
     CDS             7821..8261
                     /locus_tag="K0W65_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBI3096610.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8338..8919
                     /gene="rsxA"
                     /locus_tag="K0W65_003488"
     CDS             8338..8919
                     /gene="rsxA"
                     /locus_tag="K0W65_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBI3096611.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8919..9497
                     /gene="rsxB"
                     /locus_tag="K0W65_003489"
     CDS             8919..9497
                     /gene="rsxB"
                     /locus_tag="K0W65_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBI3096612.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9490..>11462
                     /gene="rsxC"
                     /locus_tag="K0W65_003490"
     CDS             9490..>11462
                     /gene="rsxC"
                     /locus_tag="K0W65_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBI3096613.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAA"
BASE COUNT         2681 a         2964 c         2898 g         2919 t
ORIGIN      
        1 tggtgtaata acggatttca gggcatcggt taatattgcc cccggcgcaa tgctattcac
       61 ccgaatattt ttttcaccca ggtcaaatgc catatttctg accagatgac tggccgcagc
      121 tttagatgat gcataggaag tcatgtttat atttttattt tctgccgcca tagaagtgat
      181 ggtcagaata acgccacctc catttttttc catttctggc gcaacaagtt gtgacagatg
      241 gaaaaaagaa aacacattca gttcataagc acggcgaaaa tctgccattg gcatatcaaa
      301 cggtttagga ccaccgccac cggcgttgtt aaccaggata tcaactttac ccagattact
      361 gacggcaaag tctgccagtg cagagagttc ctgttcggaa gtaatatcac aacgccaggc
      421 aaatgcctga ccatccagtt gttgaatttc gtctacaaca tggttagctg cgtcggcgtt
      481 aatatcactg accaccacag atgcgccagc tgtcgcgaat gtaatggcga tttctttacc
      541 aatacctgca cccgcacctg tgatgatggc gcattttccg tcgagtctca ggttgtcaga
      601 attaaacaca taaacctcct gtgaacttca gtaaaaataa aaggcaccgc ctgtgtgcca
      661 cattttttat cattaacctt gaggaaagga cactaccaag tatagcagat aaagagagga
      721 ttatttcgcc gcaatgagcc gggcgggaat aataagattg cgtgaatgcg tctcttcatg
      781 ggtgattttt tgcatcatgc gatccgcaag tgtgatacca agttcccgcg ctggcgtgct
      841 ggcccaggta acaggtatat catcaagtgt ggttggtgtc gcatcggtaa atgccgccag
      901 cgaaacctgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc gccctgcttt
      961 cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca cggcactgat
     1021 ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct ggctggaagt
     1081 gcactccaac acccaatcgc tgtgaaacgg caggccaaat tttagtagag ttgcacaata
     1141 gccccccacc cgttctgcac gggttaatga ggaactttgc cctcccagcc aggcgatccg
     1201 ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca tgttgtccgg
     1261 gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca ccgggatagc
     1321 tttttcttct gccatccgcc gcaggtcatc gctgcttcct gcagccccgg caattaccac
     1381 accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct cgccgtcttt
     1441 accgccgtga agcaaaaaaa ccatccgtcc ctgtgcttcc agagcttccg tcaatccggc
     1501 cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa tgacgccgct
     1561 ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct cttcaatggc
     1621 ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccattaa gcaccagcga
     1681 aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta tttttttggc
     1741 ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt ttacctttta
     1801 tttatcttat acccgctatt atcgttgcgt aatgtgattt atgactcact aaaatttgat
     1861 aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac gaggagtcgt
     1921 tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca gcagttaggc
     1981 aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct cggcattggt
     2041 agttctctta gcagccatga tgtcataacc ctgatcccgg tcctgggcaa ccccgtgttg
     2101 caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt cctgcctgtc
     2161 atgttctgta tcgccatccc gctgggcctg gcacgcgaaa ataaaggcgt agcggcattc
     2221 gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt gaccaataaa
     2281 ggcattctgc caaccacaga tgccgccgtt ctgaaagcca ataacatcca gagcattctt
     2341 gggatccagt cgatcgatac cgggatcctc ggtgcggtga tcgccggtat tatcgtctgg
     2401 atgctgcatg agcgtttcca taatatccgc ctgccggatg cgctggcatt cttcggcggt
     2461 acgcgcttcg taccaattat ctcctcgctg gtgatgggcc ttgtcggcct ggtgattcca
     2521 ttagtctggc cgattttcgc catgggtatt agcggcttgg gccatatgat aaacagcgcg
     2581 ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc gtttggtctg
     2641 catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca ggaggtctgc
     2701 ggtcaaaccg tcagcggcgc actgaccatc ttccaggcgc aattgagttg cccgaccact
     2761 cacggttttt ctgaaagcgt cacgcgtttc ctttcgcaag gtaaaatgcc tgcgtttctc
     2821 ggcggtctac caggtgcagc gttagctatg tatcactgcg cgcgcccgga aaatcgccat
     2881 aaaattaaag gtctgctgat ttctggcctg atcgcctgcg tcgttggcgg cactaccgaa
     2941 ccgctggaat tcctgttcct gttcgtagcg ccagttctgt atgtcatcca cgcgctgtta
     3001 accggcctcg gcttcaccgt catgtctgtg ctcggcgtca ccatcggtaa taccgacggc
     3061 aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa gtggtacatg
     3121 gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg tttcgctatc
     3181 acccgcttca atctgaaaac cccggggcgc gatagcgaag ttgccagctc aatcgaaaaa
     3241 gccgttgccg gtgcgccggg taaatcaggt tacaacgttc ctgcaatcct cgaagcatta
     3301 ggcggtgccg acaatattgt cagcctcgat aactgcatta cccgtctgcg tttgtctgtg
     3361 gaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat tggcgtagta
     3421 caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc agtaaaagat
     3481 gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt cgatttttca
     3541 aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc tgaccgtttc
     3601 ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac tgccccctgc
     3661 attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag ccgctggaaa
     3721 aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta caccgcaatt
     3781 gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga actgattcgt
     3841 cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga cgcattttac
     3901 aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa gcaggctgac
     3961 ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga atgtaaaatt
     4021 atgctcctgt gtaacccaca gaatcctacc gggaaagtgt ggacgtgcga tgagctggag
     4081 atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga aatccatatg
     4141 gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg gggagactgg
     4201 gcgttgctga catcgggatc gaaaagtttc aatattcccg cgctgaccgg tgcttacggg
     4261 attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg acgtgatggg
     4321 ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca gcaaggtgca
     4381 ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat ctcagataaa
     4441 atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta tctggcctgg
     4501 cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct tatcgagcaa
     4561 gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg ttttgtccgt
     4621 ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt aataaacgcc
     4681 atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc aatcgtggat
     4741 ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa agagtgcgac
     4801 catgattgat accaccctgc cattaactga tatccatcgc caccttgatg gcaacattcg
     4861 tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg cacaatccct
     4921 tgaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg tgagctttct
     4981 gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc gccgcgtggc
     5041 atttgaaaac attgaagatg cagcccgtaa cggcctgcgc tatgtcgagc tgcgtttttc
     5101 accaggctac atggcaatgg cacataagct gcctgtagcg ggtgttgtcg aagcggtgat
     5161 cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta tcggcattat
     5221 gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt tagcccaccg
     5281 tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg gaagtctgtt
     5341 cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc atgcaggcga
     5401 agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg agcgtattgg
     5461 acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg agcaacaaat
     5521 tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag agctggctgc
     5581 acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca ctgacgatcc
     5641 cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag ctgctgggtt
     5701 atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt tcctcagcgc
     5761 agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat ggatggtgca
     5821 aattgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg cggattcgat
     5881 gcccagctca atcaactcca ttacctggat tgcctggctt gccggaaccg gattttcacc
     5941 atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc caggcaccgt
     6001 caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc catcacgcat
     6061 atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt cttcctgtgg
     6121 atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc gtgctgactc
     6181 agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg acaagatggc
     6241 gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta ccgtcatgct
     6301 gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta aatcgtacca
     6361 gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg gacggaagcg
     6421 gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga gcaaaccttt
     6481 tagcgtcaag aaatcgctat cccaacggcg gttatggaat acagacagca cacgccccag
     6541 gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa agggtttatc
     6601 aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat gggtatcgtt
     6661 gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct tcggctcaga
     6721 gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc tggagattac
     6781 cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt tgctcgcata
     6841 accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc tcattgaagt
     6901 atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac ctgccagcaa
     6961 taagggatgt tgtttaactt aagtcaaaaa aatagcgaat tttccaacga caaaagctaa
     7021 atatcgcaaa aacctcagta aaaatcttgc tggagctatt attgctaagt aacatttacc
     7081 ccctgaagtt aatggatcaa tcaagagaga tgtgggctgt aatgaatcgt cttattgaat
     7141 taacaggttg gatcgttctt gtcgtttcag tcattcttct tggcgtggcg agtcacattg
     7201 acaactatca gccacctgaa cagagtgctt cggtacaaca caagtaagct ctgcacttgt
     7261 ggagcgacat gctgcccgtc cgggtgcatg ttttcacttg tcggatatta aaccaggaat
     7321 ttattatctt gttcgatgtt gttggtgatt gtcagggata gtaaagcgcg ttactctttt
     7381 cagagcagtt tttcatccct gaatatttgc catcagcgca ttcttattat tgtaataaca
     7441 caagcgttca cttttggtga aattttatca tcactaataa tacctacccc tttggtatta
     7501 attattttat ggacctttta tgactgttca ggactactta ttaaaatttc gcaaaatcag
     7561 ttcactcgaa agtctggaaa aactctacga ccatcttaat tacaccctga cggacgatca
     7621 ggaactgatc aatatgtatc gtgctgccga tcaccgtcgc gcagagctgg tttctggcgg
     7681 gcgtttgttt gacctcggcc aggtaccgaa gtccgtctgg cactatgtcc aataaagaaa
     7741 gtagcgatcg gttataaaag ccttataata acgcccctaa aattagattt gcctgtttgc
     7801 gtcgctattc tggagaacct atgaccacca caacgccaca acgtattgga ggctggttgc
     7861 ttggcccgtt ggcctggctt ttagtcgctc tgttgagtac gacgctggcg ttgttgctgt
     7921 ataccgctgc gttatcttct ccgcaaacat ttcaaacgct cggcggacaa gcactgacga
     7981 cacaaatctt atggggcgtt tcttttatta ccgctatcgc catgtggtat tacacactgt
     8041 ggttgaccat cgctttcttt aaacgtcggc gttgtgttcc taaacactat attatctggc
     8101 tgcttatttc cgtattgctg gcagtaaaag cattcgcttt ttcacccgtg gaagttggca
     8161 tagccgttcg ccagttgctg tttactttgt tggcaaccgc attgatcgtc ccctatttca
     8221 aacgctcgtc gcgggtaaaa gcgacgtttg tgaatccgta ataaccttac agttaacctg
     8281 ttgtcgcctg ctctggatta acggataata ggcggctttt ttatttcagg ccgaaaaatg
     8341 actgactacc tgttactgtt tgtcggaact gtactggtca ataactttgt actggtcaag
     8401 tttctcggtc tctgtccgtt tatgggggtt tccaaaaaac tggaaaccgc gatgggcatg
     8461 gggctggcaa caacgtttgt gatgacgctg acgtctattt gcgcctggct tatcgatacg
     8521 tggattttga tcccacttaa tctgatttac ctgcgcaccc tggcatttat tctggtgatt
     8581 gctgtggtcg tgcagttcac cgagatggtg gtgcgcaaaa ccagcccggt gctttaccgt
     8641 ctgctgggga tttttttgcc gcttatcacc accaactgtg cagtgctcgg cgtggcgttg
     8701 ctgaatatca atctcgggca caatttcttg cagtcggcgc tgtacggttt ttccgccgct
     8761 gtcggtttct cgctggtgat ggtgctcttc gccgccatcc gcgaacgcct tgctgtggct
     8821 gatgtcccgg caccttttcg cggtaatgcc attgcgttaa ttaccgcagg tcttatgtct
     8881 ctggccttta tgggctttag tggtttggtg aagttgtaat gaatgctatc tggattgccg
     8941 ttgccgccgt gagcctgctg ggcctggcgt ttggcgccat tctgggttat gcctcccgcc
     9001 gttttgcggt ggaagacgat ccggtcgttg agaaaattga cgaaatctta ccgcagagcc
     9061 agtgtggtca gtgcggttat cccggctgtc gcccctacgc ggaagccatc agctgtaacg
     9121 gtgaaaaaat caaccgttgc gccccaggtg gcgaagctgt gatgctaaaa attgccgagt
     9181 tgcttaatgt cgagccgcag ccgctggatg gcgaagcgca agagctaacg cctgcgcgga
     9241 tggtggcggt tattgatgaa aataactgta ttggctgcac taaatgtatt caggcttgtc
     9301 cggtagacgc catcgttggc gctacccgtg tcatgcatac ggtaatgagt gatctctgta
     9361 cgggctgcaa tttatgtgtt gatccgtgcc cgacgcactg catctcgttg caaccggtcg
     9421 cagaaacacc tgactcctgg aaatgggatc tgaacaccat tcccgtgcgt atcattcccg
     9481 tggaacacca tgcttaagtt attctctgca ttcagaaaaa ataaaatctg ggatttcaac
     9541 ggcggcatcc atccaccgga gatgaaaacc cagaccaacg gtacccccct gcgccaggta
     9601 ctactggcgc agcattttgt tattccactg aaacagcata ttggcgctga aggtgagttg
     9661 tgcgttagcg tcggcgataa agtattgcgc ggccagccgc ttacccgtgg tcgcggcaaa
     9721 atgctgcctg ttcacgcgcc cacctcgggt accgttacgg ctattgcgcc ccactctacg
     9781 gctcatcctt cagctttagc tgaattaagc gtgattattg atgccgatgg tgaagactgc
     9841 tggatcccgc gcgacggctg ggtcgattat cgctctcgca gtcgcgaaga gttaatcgag
     9901 cgcatacatc agtttggtgt tgccgggctg ggcggtgccg gattcccgac aggcgttaaa
     9961 ttgcagggtg gtggagataa gattgaaacg ttgattatca acgctgctga gtgcgagccg
    10021 tacattaccg ccgatgaccg tttgatgcag gattgcgcgg ctcaggtcgt agagggtatt
    10081 cgcattcttg cgcatattct gcagccacgc gaaattctta tcggcattga agataacaaa
    10141 ccgcaggcga tttccatgct gcgcgcggtg ctggcggact ctaacgatat ttctctgcgg
    10201 gtgattccaa ccaaatatcc ttctggcggt gctaaacaat taacctacat tctgaccggg
    10261 aagcaggttc cacatggcgg gcgttcatcc gatatcggcg tattaatgca aaacgtcggc
    10321 actgcttatg cagtgaaacg tgccgttatt gatggcgagc cgattaccga gcgtgttgta
    10381 accctgactg gcgaagcaat cgctcgcccg ggcaacgtct gggcacggct ggggacgcca
    10441 gtgcgtcatt tattgaatga tgccggattc tgcccctctg ccgatcaaat ggtgattatg
    10501 ggtggcccgc taatgggctt taccttgcca tggctggatg tcccggtcgt aaagattacc
    10561 aactgtctgt tggctccctc tgccaatgaa cttggcgaac cacaggaaga acaaagctgc
    10621 atccggtgta gcgcctgtgc tgacgcctgc cctgtggatc ttttgccgca acagttgtac
    10681 tggttcagca aaggtcagca acacgataaa gctaccacgc ataacattgc cgactgcatt
    10741 gaatgtgggg cttgcgcgtg ggtttgcccg agcaatattc ccttggtgca atatttccgt
    10801 caggaaaaag ctgaaattgc ggctattcgt caggaagaaa agcgcgccgc agaagccaaa
    10861 gcgcgtttcg aagcgcgcca ggctcgtctg gagcgcgaaa aagcgactcg ccttgaacga
    10921 cataagagcg cagccgttca acctgcagcc aaagataaag atgcgattgc tgccgctctg
    10981 gcgcgggtga aagagaaaca ggcccaggct acacagccta ttgtgattaa agcgggcgaa
    11041 cgcccggata acagtgcaat tattgcagaa cgggaagccc gtaaagcgca agccagagcg
    11101 aaacaggcag aactggagca aactaacgac gcagcaaccg ttgctgaacc acgtaaaact
    11161 gccgttgaag cagctatcgc ccgctccaaa gcgcgcaagc tggaacagca acaggctaat
    11221 gcggaaccag aagaacaggt cgatccgcgc aaagccgccg tcgaagccgc tattgcccgt
    11281 gccaaagcgc gcaagctgga acagcaacag gctaatgcgg aaccagaaga acaggtcgat
    11341 ccgcgcaaag ccgccgtcga agccgctatt gcccgcgcca aagcacgcaa gctggaacag
    11401 caacaggcca atgcggagcc agaagaacag gttgatccgc gcaaagctgc cgtcgaagcc
    11461 gc
//
LOCUS       DAETQL010000113        11112 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.113, whole genome shotgun sequence.
ACCESSION   DAETQL010000113 DAETQL010000000
VERSION     DAETQL010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.113"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..460)
                     /gene="intS"
                     /locus_tag="K0W65_003491"
     CDS             complement(<1..460)
                     /gene="intS"
                     /locus_tag="K0W65_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HBI3096614.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(622..696)
                     /locus_tag="K0W65_003492"
     tRNA            complement(622..696)
                     /locus_tag="K0W65_003492"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(661..663),aa:Arg,seq:cct)
     gene            complement(770..1702)
                     /locus_tag="K0W65_003493"
                     /pseudo
     CDS             complement(770..1702)
                     /locus_tag="K0W65_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1996..2751
                     /gene="mlaA"
                     /locus_tag="K0W65_003494"
     CDS             1996..2751
                     /gene="mlaA"
                     /locus_tag="K0W65_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBI3096615.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2933..3991)
                     /locus_tag="K0W65_003495"
     CDS             complement(2933..3991)
                     /locus_tag="K0W65_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096616.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4357..5697)
                     /gene="fadL"
                     /locus_tag="K0W65_003496"
     CDS             complement(4357..5697)
                     /gene="fadL"
                     /locus_tag="K0W65_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBI3096617.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6069..6353
                     /locus_tag="K0W65_003497"
     CDS             6069..6353
                     /locus_tag="K0W65_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HBI3096618.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6533..7843
                     /gene="fadI"
                     /locus_tag="K0W65_003498"
     CDS             6533..7843
                     /gene="fadI"
                     /locus_tag="K0W65_003498"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBI3096619.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7843..9987
                     /gene="fadJ"
                     /locus_tag="K0W65_003499"
     CDS             7843..9987
                     /gene="fadJ"
                     /locus_tag="K0W65_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HBI3096620.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10190..10675
                     /gene="sixA"
                     /locus_tag="K0W65_003500"
     CDS             10190..10675
                     /gene="sixA"
                     /locus_tag="K0W65_003500"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBI3096621.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2775 a         2545 c         2748 g         3044 t
ORIGIN      
        1 ttgcaccgcg atcttcaaat ctgcggatta cttccagcag ttgcatcggc tcaatatcct
       61 gaatctcaag gccgccgatg atgggtaaaa tgtcgtcatc aaacattttt gcaagttcag
      121 tcgcataccc tactgaccag acttgcttct tgtgttcgta ccattccttg taaatcgcac
      181 taaatgaatt gttgttagac gaagcctttt tcgcctttac cggatcgatg ccagccgaga
      241 tgtctttcct cgcagtccat gctttatccc ttgcctcctg caaagtcata agcggatatt
      301 ttccgacggt caggattttc tccttaccgt caatcttgta gcgaagctgc catacctttt
      361 tcccggatac agggacataa aggtacaggc cattaccatc gagaaggcgg tatggttttt
      421 ctttcggctt tgctgcttca atctgcttaa cggtgagcat gggtaaaaat ccggtgggta
      481 aaattatttt atccactttt tacccgtcat ggagtgcggc tgtcaacgat ctgacgcgaa
      541 ccatgacgaa ctgtgaatat acggaaggct tgatattcag gggattttgc ggactggtac
      601 ggatgggagc gaactgataa atggtgtccc ctgcaggaat cgaacctgca attagccctt
      661 aggaggggct cgttatatcc atttaactaa gaggacaatg cggcgagtat acccgctaat
      721 ggactgcggg gtaagtacgc tgccgctcga ttgcttaaac cctcgccatt tatgctgggt
      781 ttttatcatt tttcttaatg ttttccgcac gttctgcttt ttggcgtgct tctgctttac
      841 gcttgttgct catgtcgtta cgaatctgtg catgactcat taacgcgaag ataaaggtgc
      901 cgccgcagat gttccccgct aaagtaggta gtgcgaaggg ccagatgaaa tcgctccagt
      961 gcagcgtacc gttaaacacc agatagagga tttcaacaga accgaccacg atatgggtgg
     1021 tgtcacccag ggcaataagc caggtcatca atataatcac cacaatcttt gccgcacctg
     1081 ctgcaggaaa catccatacc atagtggcga tcagccagcc ggaaatgatc gcgttggcaa
     1141 acatctcgct gggggtgttc ttcatcacat ccatgccgat tttgacaaat gcatcgcgag
     1201 tttcttcatt gaagataggc atatattcaa atgcccacgc agcaatacca gtcccgagaa
     1261 tattacccag cagcacgact ccccataacc gcataagtaa gccaacgttg ctcattgtcg
     1321 gtttttgcat gacgggtagt accgcagtca cggtgttttc ggtaaataat tgctggcggg
     1381 ccattatgac gataataaaa ccaaaggtat aaccgagatt ctccagcaag aagctgcccg
     1441 gcacaccttc cagttcggca tgaaatatcc cttttgccag taacgaagcg cccatcgaca
     1501 gacccgccgc aatggctgac cacagtagcg ccattgcgtc gcgttctagc tctttttcac
     1561 catcctggcg gatatgctca tgaattgcca tcgctcggga ggggagtcgg tcttcatcta
     1621 tttctatttt tttgccgcgc tctttttctt cgctctaaac ttcaatttcg tcgctgtgtt
     1681 gatcaatttt gtcgttgtcc atggtctctt ctcttgaatt agcacgtata gctaaagcgt
     1741 agcggctttt ttgctcgcaa ctggcgggag ttactctgaa aatgtagaaa aggctgcgtt
     1801 tgcctttttc tgtttctata gaatcaagta gcctacaggg cggcgattac caggctatga
     1861 tcaaatcagc aaatcagggc gtctggacat cagttgacgt gctgttacaa tcgcccacac
     1921 ctaaacaggc ggatacggta tcgttccgtc atggatggca aactgcataa gccataaaaa
     1981 aacagggaga catttatgaa gcttcgcctg tcggcgcttg ctctgggaac tacgcttctg
     2041 gtggggtgtg cgagttccgg tacagatcag caagggcgtt ctgacccgtt agaagggttc
     2101 aaccgcacca tgtacaactt caacttcaac gtattagacc cgtatattgt tcgaccggtc
     2161 gctgtcgcct ggcgtgatta tgttccgcaa ccggcgcgta atggtttgag caactttact
     2221 ggcaaccttg aagaacctgc ggtgatggtt aactacttct tgcagggcga cccttatcag
     2281 gggatggtcc actttacccg ctttttcctg aacaccattt tggggatggg cggctttatt
     2341 gatgttgcag ggatggcgaa cccgaaactg caacggactg aacctcaccg cttcggtagt
     2401 acgcttggtc attatggcgt gggttatggg ccttacgttc agttaccgtt ctacggtagc
     2461 ttcacgctgc gtgatgacgg tggtgatatg gcggatgctc tttacccggt tctttcctgg
     2521 ctgacctggc cgatgtctgt gggtaaatgg acgcttgaag ggatcgaaac ccgcgctcag
     2581 ctgctggatt ccgatggtct gctgcgtcag tcgtccgatc cttatattat ggtgcgcgaa
     2641 gcgtacttcc agcgtcatga tttcatcgct aatggcggcg aactcaaacc gcaggaaaat
     2701 ccgaacgcgc aagcgattca ggatgattta aaagatatcg attctgaata agaaacaaat
     2761 aaaaaaggtg agtcgcaaaa ctcacctttt ttgttgttat ccctcaatta ccacaagtaa
     2821 aaggagataa accgacaatg acccggacgt aatgatgtaa aaatactgat gtttgtcact
     2881 gactatttcc gatattcagg cgttgaaaaa tcaaaaaact ggaaaaataa ttttaaatat
     2941 catcaaaatt aacacctagt gcagatagtt gattaatttc attttgatat ttcgtctggc
     3001 gattttgaag tcttttttca actttatctt cgagattctt aaagtctttg tatttatttt
     3061 caggatggtc aacgtcaact ttgttagcga ataaagtaga aattatttct tttacattat
     3121 cgcgtctaaa ttttgcatcg tccacttgaa aattatatct tattgctcga cctaaacgtt
     3181 taaaaacttc gttaggttct ctcagtcttt ccccgttgag ctcgtttaca acctgatgat
     3241 cggaaccagt tgcaaatgat tctgccatga tagctttact gctttcaaac cacgctgaag
     3301 cactcctggc gttttcacta caatattggg tgagactatc accattaata cgaattcctc
     3361 tttgaatgtg gtagtcgtcc tgatcgtagt aaccgagaga taagtaatca tttccgtttt
     3421 tatttttaat tacaatagcg agagagtcgc cttttttatc tttaccgtac caggtaaata
     3481 agtccgttgg ttcgcttttc ccgctctcat caaattccgt taccgtatgt ctgagtttgt
     3541 gtgatatctt atcgaagata aaaccaggat ctccatcata tatttctttt aatgcgctga
     3601 agatcatttc cttgttattg atatcatgag tcttctcaac tatgttgtca ttaatgatag
     3661 tacggacgag ttctttgtct ttatagttcg caggtaattc tattggttct tggttgtcga
     3721 taccaatagt aatatggtct gtatcattgg agccaatatc aatataagta actgaggaat
     3781 tattaatatt agatctataa ggatcgtcgt ttactcgtat ggcgtttttt ataagctcaa
     3841 gtagatgttt aatacaggtg cttattcctt ccttaccatt aaaaaggttt ttgaactctt
     3901 gtactaatcc attaatttca tcattgttat tttcattgat ggattctggg taagctgcgc
     3961 tggtattgtt gattgaaata gatggtagca tgattttatt ccatgttgat tgatttgtta
     4021 caaatatata ttacctaatg aaatagacta tcaaaaaacg ctcatttatt tagaacctat
     4081 ctttaatttg agtgtataag cgcacaatag ccaggggggt tgagtatcct ggaaatgcag
     4141 aaaagtaaac tctttgagaa ggacgagcag aacgcttgaa aatatgcatt tcccttcagg
     4201 tttgtctgtg gataccactt actgatttga aagggcaagc tgccatcagg ctgtttgcaa
     4261 aactcaggag gcaagcaatg aggaaactac tttcggtgaa gtgggggagt taaagtcacc
     4321 tgcatagcag gtgactttat ccaggcgaac gcgttatcag aacgcgtagt taaagttagt
     4381 accgaacagc caggctttac cttcagactc gaactggtat gggccttcgt taattttcac
     4441 gctctgaccg tgcatataag aaacaccaac gtcgactgaa gcatctttat taaatgcgta
     4501 agtcgtacct gcactcagcc agaaacggtc ctggtccgga atggagatag aacgattctg
     4561 agccggaact gggctgtcat caaaggcgat accggtacgg aaggtccagt tatcatcgta
     4621 gtaataagtg gtacccaacg cgatgcggta agcatcttta aagccttcat gtttctggaa
     4681 cagcgtgtcg ccactggttg aggtcgcttt cagctgctgg aactgactcc agctggtgta
     4741 agccaggcta tagtgaatcg cccactgtgg atcaacacgg ttataacctg acacttccca
     4801 catttcaggc aggttcagcg tcagataacc cgattgcgtt gcgccacctg tcgcggtagg
     4861 aattggcaaa ccgtagttat taaacgcacg attaagatcg ctgctgtagt tacctttgaa
     4921 gtcaattttc acttcagaac ggtaggtcag tgcatagcgg ttatttttat ccagttcata
     4981 caggattccg gcgttccagc caaagcccca ctggttaccg ttcagatgag cgattttggt
     5041 attactgtcg ataccgttgg cggtagctgc caatgcttgc ccttgaggag tttgcccggc
     5101 aggagattgc ataatctgac cagcaaccaa ctgccccaga tcgcctgcga aacgttcaat
     5161 tttcgcgcga gcgtagatgg cgttgaaacc aagaccaaag ctccatgcat tatttaagcg
     5221 atacgcaccg cttaagttca ggttcatggt ttcaaggtca gttgtacccc cgacagagcc
     5281 gcctgcataa gtatcattaa actctgttgc cagaccatag ttagaggtaa tagaagcgcc
     5341 ccaaccaaat tggtcgttaa tcggtgcaac aaagtgcatg ttcggaaccc atgccgtagg
     5401 cgcgatgtta tcggctttca ggctacgacc agacggagac gttccgctga tattcacatc
     5461 cgggtcaata taaaccgcac ccgcagaaaa tgtcgggcgg tcaaacatgg taatcaatgc
     5521 ggggttacgg ctaacgttac ctgcatcatc ggcaattgcg ccttcccctg aataagcccg
     5581 gcccaggcca gaggaagaaa attcgtttaa ctgaaagcct gccgaccagg cctgggtgga
     5641 gataagtgcc actgcgactg cgagagcaga ctttgtaaac agggttttct ggctcatgac
     5701 cataacctca atgatttatt tttatacaaa ctatgttacg tgctgtaaca ggagcgcgaa
     5761 gtgtagggtc tgaggtacat cttagaaatc agaccagtgg cgagagtata ggtcggacca
     5821 gctagaatgt tgcaagtatg tttccatctt ttttcaaata tgatctaaga aacgcgatcc
     5881 agctggcaaa atttggaggt tgcgcactcg cggattcggg tgaattttgt tttagatcat
     5941 ttttaagtgt gatttcggtc acttatccga ttttgcagaa ttaacaactg gagcagcact
     6001 ttccggaagc gtaaaatatc cacaacgttt atctggcacc ttgggtgcac atacagagga
     6061 aatcaacgat gagtaaatgt agtgctgatg aaaccccggt ttgctgctgt atggatgttg
     6121 gcaccattat ggacaactcc gactgcaccg cgtcttacag ccgcgtgttt gcaaaccgcg
     6181 cagaagcaga acaaacgctg gcggcgctga ctgaaaaatc ccgtagcgtg gaatccgaac
     6241 cgtgcaaaat ctccccgact ttcactgaag agtctgacgg tgtgcgcctg gatatcgatt
     6301 tcacttttgc ctgcgaagct gaaatgctga tcttccagct gggtctgcgt taattcctct
     6361 cgggtacgta cgcccctgtc agttgctggc aggggcgttt tcttttcttt cttctgtctt
     6421 cactgtgtca tagctcccac tttccttttt ctccgcttgg ctaaatgtaa aaaaatggtt
     6481 aagactatga tcaggtcaga ccactttatt tattttttta caggggagtg ttatgggtca
     6541 ggttttacca ctggttaccc gccagggcga tcgtatcgcc attgttagcg gtttacgtac
     6601 gccttttgcc cgccaggcga cggcttttca tggcattccc acggttgatt tagggaagat
     6661 ggtggtaggc gaactgctgg cacgcagcga gatccccgct gaagtgattg aacagctggt
     6721 ctttggtcag gtcgtacaaa tgcctgaagc ccccaacatt gcgcgtgaaa ttgttctcgg
     6781 taccggaatg aatgtgcata ccgatgctta cagcgtcagc cgcgcttgcg ctaccagttt
     6841 ccaagcggtt gcaaacgtcg cagaaagcct gatggcggga actatccgag cggggattgc
     6901 cggtggggca gattcctctt ccgtattgcc aattggcgtc agtaaaaaac tggcgtacgt
     6961 gctggttgat gtcaacaaag ctcgaaccat gagccagcgc ctgaaactct tctctcgcct
     7021 gcgtttgcgc gacttaatgc ccgtaccgcc tgcggtagca gaatattcta ctggcttgcg
     7081 gatgggcgac accgccgaac aaatggcgaa aacctacggc atcacccgag aacagcaaga
     7141 tgcattagcg caccgttcgc atcagcgtgc cgctcaggca tggtcagacg gaaaactcaa
     7201 agaagaggtg atgactgcct ttatccctcc ttataaacaa ccgcttgtcg aagacaacaa
     7261 tattcgcggt aattcctcgc ttgccgatta cgcaaagctg cgcccggcgt ttgatcgcaa
     7321 acacggaacg gtaacggcgg caaacagtac gccgctgacc gatggcgcgg cagcggtgat
     7381 cctgatgact gaatcccggg cgaaagaatt agggctggtg ccgctggggt atctgcgcag
     7441 ctacgcattt actgcgattg atgtctggca ggacatgttg ctcggtccag cctggtcaac
     7501 accgctggcg ctggaacgtg ccggtttgac gatgagcgaa ctgacattga tcgatatgca
     7561 cgaagccttt gcagctcaga cactggcgaa tattcagttg ctgggtagtg aacgttttgc
     7621 tcgtgaagta ctggggcgtg cacatgccac tggcgaagtg gacgatagca aatttaacgt
     7681 gcttggcggt tcgattgctt acggacatcc cttcgcggcg accggcgcgc ggatgattac
     7741 ccagacattg catgaacttc gccgtcgcgg cggtggattt ggtttagtta ccgcctgtgc
     7801 tgccggtggg cttggcgcgg caatggttct ggaggcggaa taatggaaat ggcatcagcg
     7861 tttactctta atgttcgtct ggacaacatt gctatcatca ccatcgacgt accggatgag
     7921 aaaatgaata ccctgaaggc ggaatttgcc tcgcaggtgc gcgccattat taagcaactc
     7981 cgtgaaaaca aagagttgcg cggcgtggtg tttatctccg cgaaaccgga caattttatc
     8041 gccggagcag acatcaacat gattggcaac tgcaaaacgg cgcaagaagc ggaagcactg
     8101 gcgcggcagg gccaacagtt gatggcggag attcatgctt tgcccattcc ggttatcgcg
     8161 gctattcatg gcgcttgcct gggcggtggg ctggagctgg cgctggcgtg ccacggtcgc
     8221 gtttgtactg acgatcctaa aacggtgctc ggtttgcctg aagtacaact tggattgtta
     8281 cccggttcag gcggcaccca gcgtttaccg cgtctgatag gcgtcagtac agcattagag
     8341 atgattctca ccggaaaaca acttcgggcg aaacaggcat taaagctggg gctggtggat
     8401 gacgttgttc cgcactccat tttgctggaa gccgctgttg agctggcgaa gaaggatcgc
     8461 ccatcttccc gccctctacc tgtacgcgag cgtattctgg cggggccgtt aggtcgtgcg
     8521 ctgctgttca aaatggtcgg caagaaaaca gaacacaaaa ctcaaggcaa ttatccggcg
     8581 acagaacgca tcctggaggt tgttgaaacg ggattagcgc agggcaccag cagcggctat
     8641 gacgctgaag ctcgcgcctt tggcgaactg gcgatgacgc cgcaatcgca agctttgcgt
     8701 agtatctttt ttgccagtac ggacgtgaag aaagatcccg gtagtgatgc accgcctgcg
     8761 ccattaaaca gcgtgggaat tttaggtggt ggcttgatgg gcggcggtat tgcttatgtc
     8821 actgcttgta aagcggggct tccggtcaga attaaagata tcaacccgca gggcataaat
     8881 catgcgctga agtacagttg ggatcagctg gagggcaaag ttcgccgtcg tcatctcaaa
     8941 gccagcgaac gtgacaaaca gctggcatta atctccggaa cgacggacta tcgcggcttt
     9001 gcccatcgcg atctgattat tgaagcggtg tttgaaaatc tcgaattaaa acaacagatg
     9061 gtggcggaag tggagcaaaa ttgcgccgct cataccattt ttgcttcgaa cacgtcatct
     9121 ttaccgattg gtgatatcgc cgctcacgcc gcgcgacctg agcaagttat tggcctgcat
     9181 ttcttcagtc cggtggaaaa aatgccgctg gtagagatta ttcctcatgc gggtacatcg
     9241 gcgcaaacca tcgccactac ggtaaaactg gcgaaaaaac agggcaaaac gcccattgtc
     9301 gtgcgtgaca aagccgggtt ttacgtcaat cgcatcttag cgccttacat taatgaagct
     9361 atccgcatgc tgaccgaagg tgaacgggta gagcacattg atgccgcgct agtgaaattt
     9421 ggttttccgg taggcccaat ccaacttttg gatgaggtag gaatcgacac cgggactaaa
     9481 attattcctg tactggaagc cgcttatgga gaacgtttta gcgcgcctgc aaatgttgtt
     9541 tcttcaattt tgaacgacga tcgcaaaggc agaaaaaatg gtcggggttt ctatctttat
     9601 ggtcagaaag ggcgtaaaag caaaaaacag gtcgatcctg ccatttaccc gctcattggc
     9661 gcacaagggc aggggcgact ctccgcaccg caggttgctg aacggtgtgt gatgttgatg
     9721 ctgaatgaag cagtacgttg tgttgatgag caggttatcc gtagcgtgcg tgacggggat
     9781 attggtgcgg tatttggcat tggttttccg ccatttctcg gtggaccgtt ccgctatatc
     9841 gattctctcg gcgcgggcga agtggttgca ataatgcaac gactggccac gcagtatggt
     9901 tcccgtttta ccccttgcga gcgtttggtc gagatgggcg cgcgtgggga aagtttttgg
     9961 aaaacaactg caactgacct gcaataagaa ggtcaaagct atatgaatcc gcgctgaatg
    10021 gcggagtgtt ggtcaaaatg taaacgcata ttgactatac ttacgccatt gaggtaaaaa
    10081 acagcgtttc attcggtgaa tggataaggc acaatgccgg ccaccgtttt ctttctctgg
    10141 tttcagatga aagaaaacgg gcgaatctgg ttaacaaaag cggtgcaata tgcaagtttt
    10201 tatcatgcgt cacggcgacg cagccctcga ttccgccagt gattcggttc gtcctctgac
    10261 cactaatggt tgtgacgaat ctcgcctgat ggcgaactgg ctgaaaggtc aaaaagtgga
    10321 aatcgaacgc gttctggtga gcccgttcct gcgagccgag caaacactgg aagaagtagg
    10381 ggattgtctg aacctgcctt ccagtgcaga agttctgccg gagttaacgc cctgcggcga
    10441 tgttggtctg gtcagtgcct atttgcaggc gttgaccaat gaaggtgttg cttcggtact
    10501 ggtgatctct cacttaccgt tagtcgggta tctggttgcc gagttatgtc cgggcgaaac
    10561 gccgcctatg tttaccacct cggccattgc cagcgtaacg cttgatgaaa gcggaaacgg
    10621 tacattcaac tggcagatga gcccgtgtaa tctgaagatg gcaaaagcta tctgatgtgt
    10681 tccaaattat gcccaatgag gtagattcat tgggcataat tgctcattag gcagcggata
    10741 tctgtcaacc tcactactat cgaagactct ccttatctat aaataaaaca ctttctacat
    10801 tcattcggaa attattcttc tacgcgtatc ttgattttgc ccaaagttac atgttgaaat
    10861 gaataatatc tgtttgacac tcattgtcat ttattaatac agataataga gaaaatagag
    10921 ttgaataatc aggcaggatg taattgaaac ttgattaaat tctggctata gtgtttatat
    10981 attatattgt caaaagtgga taaatgccaa tcgcattata tgttcattgt ttgtttttta
    11041 agctgttgat attcattatt gatatacttt ctattttttc taaatactta ttccgtattt
    11101 tttaaggatg tc
//
LOCUS       DAETQL010000114        11097 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.114, whole genome shotgun sequence.
ACCESSION   DAETQL010000114 DAETQL010000000
VERSION     DAETQL010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.114"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            461..1861
                     /locus_tag="K0W65_003501"
     CDS             461..1861
                     /locus_tag="K0W65_003501"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBI3096622.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            2017..3552
                     /gene="gadC"
                     /locus_tag="K0W65_003502"
     CDS             2017..3552
                     /gene="gadC"
                     /locus_tag="K0W65_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBI3096623.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            3683..5002
                     /locus_tag="K0W65_003503"
     CDS             3683..5002
                     /locus_tag="K0W65_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HBI3096624.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            5300..5341
                     /locus_tag="K0W65_003504"
     CDS             5300..5341
                     /locus_tag="K0W65_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096625.1"
                     /translation="MVQLVDLARCVSF"
     gene            5379..6524
                     /gene="dosC"
                     /locus_tag="K0W65_003505"
     CDS             5379..6524
                     /gene="dosC"
                     /locus_tag="K0W65_003505"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HBI3096626.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            6449..7066
                     /locus_tag="K0W65_003506"
                     /pseudo
     CDS             6449..7066
                     /locus_tag="K0W65_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            7324..7656
                     /gene="ddpX"
                     /locus_tag="K0W65_003507"
                     /pseudo
     CDS             7324..7656
                     /gene="ddpX"
                     /locus_tag="K0W65_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            7670..8566
                     /locus_tag="K0W65_003508"
     CDS             7670..8566
                     /locus_tag="K0W65_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HBI3096627.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            8563..9537
                     /locus_tag="K0W65_003509"
     CDS             8563..9537
                     /locus_tag="K0W65_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBI3096628.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            9530..10456
                     /locus_tag="K0W65_003510"
                     /pseudo
     CDS             9530..10456
                     /locus_tag="K0W65_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(10512..10943)
                     /gene="osmC"
                     /locus_tag="K0W65_003511"
     CDS             complement(10512..10943)
                     /gene="osmC"
                     /locus_tag="K0W65_003511"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBI3096629.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2747 a         2712 c         2757 g         2881 t
ORIGIN      
        1 attgttgaaa caaatgacgg tagagaatgt taacactgcc gttaaacaat atctttctca
       61 tccggtaaat acttataccg gagtattatt gccaaaataa taacagcccc gtcaacacat
      121 cgttggcggg gattttagca atattcgcta tttttatgta ataattttat aaatgcgttc
      181 aaaataataa ccaagtacta atagtgatat tttaatacct gatttttacg tgataattca
      241 ggaggcacag aaagcgcata aaaataacaa cataaaacac cttgccacca cccaagaatt
      301 ctctattaca ttgtttttaa tgaaaaaata tcattccaac aatatctcac gataaataac
      361 attaggattt tgttatttaa acacgagtcc tttgcacttg cttactttat cgataaatcc
      421 tactttttta atgcgatcca atcattttaa ggagtttaaa atggataaga agcaagtaac
      481 ggatttaagg tcggaactac tcgattcacg ttttggtgcg aagtctattt ccactatcgc
      541 agaatcaaaa cgttttccgc tgcacgaaat gcgcgacgat gtcgcatttc agattatcaa
      601 tgatgaatta tatcttgatg gcaacgctcg tcagaacctg gccactttct gccagacctg
      661 ggacgacgaa aacgtccaca agttgatgga tttatccatt aacaaaaact ggatcgacaa
      721 agaagaatat ccgcaatccg cagccatcga cctgcgttgc gtaaacatgg ttgccgatct
      781 gtggcatgcg cctgcgccga aaaatggtca ggccgttggc accaacacca ttggttcttc
      841 cgaggcctgt atgctcggcg ggatggcgat gaaatggcgt tggcgcaagc gtatggaagc
      901 tgcaggcaaa ccaacggata aaccaaacct ggtgtgcggt ccggtgcaaa tctgctggca
      961 taaattcgcc cgctactggg atgtggagct gcgtgagatc cctatgcgcc ccggtcagtt
     1021 gtttatggac ccgaaacgca tgattgaagc ctgcgacgaa aataccatcg gcgtggtgcc
     1081 gactttcggc gtgacctaca ccggtaacta tgagttcccg caaccgctgc acgatgcact
     1141 ggataaattc caggccgaca ccggtatcga catcgacatg cacatcgacg ccgccagcgg
     1201 tggctttctg gcaccgttcg tcgccccgga tatcgtctgg gacttccgcc tgccgcgtgt
     1261 gaaatcgatc agtgcttcag gccataaatt cggtctggct ccgctgggct gcggctgggt
     1321 tatctggcgt gacgaagaag cgctgccgca ggaactggtg ttcaacgttg actacctcgg
     1381 tggtcagatt ggtacttttg ccatcaactt ctcccgcccg gcgggtcagg tgattgcaca
     1441 gtactatgaa ttcctgcgcc ttggtcgtga aggctatacc aaagtacaga atgcttccta
     1501 ccaggtcgct gcctatctgg cggatgagat cgccaaactg gggccgtatg agttcatctg
     1561 taccggtcgc ccggacgaag gcatcccggc ggtttgcttc aaactgaaag atggtgaaga
     1621 tccgggatac accctgtacg acctctctga acgtctgcgt ctgcgcggct ggcaggttcc
     1681 ggccttcact ctcggcggtg aagccaccga catcgtggtg atgcgcatta tgtgtcgtcg
     1741 cggcttcgaa atggactttg ctgaactgtt gctggaagac tacaaagcct ccctgaaata
     1801 tctcagcgat cacccgaaac tgcagggtat tgcccagcag aacagcttta aacatacctg
     1861 ataacgttta acggtaacgg tgtcccgaaa cgaacccgtt tcgggataat ttccaaagtc
     1921 tgttcactgg cattagcaac ggaaaatatt gttctgaata cgcttcagaa caaaacaggt
     1981 gcggttccga caggaatacc gttttagggg gataatatgg ctacatcagt acagacaggt
     2041 aaagctaagc agctcacatt actcggattc tttgccataa cggcatcgat ggtaatggct
     2101 gtttatgaat accctacctt cgcaacatcg ggcttttcat tagtcttctt cctgctatta
     2161 ggcgggattt tatggtttat tcccgtggga ctttgtgctg cggaaatggc caccgtcgac
     2221 ggctgggaag aaggtggtgt cttcgcctgg gtatcaaata ctctgggtcc gagatgggga
     2281 tttgcagcga tctcatttgg ctatctgcaa atcgccattg gttttattcc gatgctctat
     2341 ttcgtgttag gggcactctc ctacatcctg aaatggccag cgctgaatga agaccccatt
     2401 accaaaacta ttgcagcact catcattctt tgggcgctgg cattaacgca gtttggtggc
     2461 acgaaataca cggcgcgaat tgctaaagtt ggcttcttcg ccggtatcct gttacctgca
     2521 tttattttga tcgcattagc ggctatttac ctgcactccg gtgcccccgt tgctatcgaa
     2581 atggattcga agaccttctt ccctgacttc tctaaagtgg gcacactggt tgtatttgtt
     2641 gccttcattt tgagttatat gggcgtagaa gcttccgcaa ctcacgtcaa tgaaatgagc
     2701 aaccccgggc gcgactatcc actggctatg ttactgctga tggtggcggc aatctgctta
     2761 agctctgttg gcggtttgtc tattgcgatg gtcattccgg gtaatgaaat caacctctcc
     2821 gcaggggtaa tgcaaacctt taccgttctg atgtcccatg tggcaccgga aattgagtgg
     2881 acggttcgcg tgatctccgc actgctgttg ctgggtgttc tggcggaaat cgcctcctgg
     2941 attgttggtc cttctcgcgg gatgtatgtc acagcgcaga aaaacctgct gccagcggca
     3001 ttcgctaaaa tgaacaaaaa tggcgtaccg gtaacgctgg tcatttcgca gctggtgatt
     3061 acttctatcg cgttgatcat cctcaccaat accggtggcg gtaacaacat gtccttcctg
     3121 atcgcactgg cgctgacggt ggtgatttat ctgtgtgctt atttcatgct gtttattggc
     3181 tacattgtgt tggttcttaa acatcctgac ttaaaacgca catttaatat ccctggtggt
     3241 aaaggggtga aactggtcgt ggcaattgtc ggtctgctga cttcaattat ggcgtttatt
     3301 gtttccttcc tgccgccgga taacatccag ggtgattcta ccgatatgta tgttgaatta
     3361 ctggttgtta gtttcctggt ggtacttgcc ctgcccttta ttctctatgc tgttcatgat
     3421 cgtaaaggca aagcgaatac cggcgtcact ctggagccaa tcaacagtca gaacgcacca
     3481 aaaggtcact tcttcctgca cccgcgtgca cgttcaccac actatattgt gatgaatgac
     3541 aagaaacact aaccatgtct gaatggttat aagacaaggg agcgataatt catcgctccc
     3601 tttttcgtgc ttgctgtcgt attgacttaa ccgggataag tacgagaatg agcgcacatc
     3661 tgtttaccgg aaaccagcac atatggatat ctgctcccga aacaagaaat taacgattag
     3721 aagaccagcg atactggttg cattggcact tttactgtgt agttgtaaaa gcacgcctcc
     3781 agagtccatg gtgacaccac cagcaggttc aaagccacca gccacgacgc aacaatcgtc
     3841 acaaccgatg cgtggcatct ggctggccac ggtttctcgc ctcgactggc caccggtttc
     3901 ctcggttaac attagtaatc ccaccagccg agcccgtgta caacaacagg cgatgatcga
     3961 caaactggat catctccaac gtctcggcat aaacacggtc tttttccagg tcaagccgga
     4021 cggtaccgcc ctgtggccat cgaaaatttt gccgtggtcc gatcttatga ccggtaagat
     4081 tggtgaaaat ccgggttacg atccgctgca attcatgctc gacgaagccc acaagcgtgg
     4141 gatgaaagta cacgcctggt ttaaccccta tcgcgtatcg gttaatacga agcccggtac
     4201 tatcagggaa ctgaatagca ctctgtctca acaaccggcg agcgtctatg tgcaacaccg
     4261 tgactggatc agaacgtctg gcgatcgctt tgtcctcgac ccgggcatcc ctgaggttca
     4321 ggactggatc acatcaatag tcgctgaagt ggtttcccgc tatccggtag atggcgtgca
     4381 gtttgacgac tatttctata ctgaatcacc gggttcacgg ctaaatgata acgaaacgta
     4441 ccgtaaatac ggaggcgcat ttgcgtcaaa agcagactgg cggcgcaaca atactcagca
     4501 gttaattgca aaggtatcac acaccattaa aagcattaag ccgggagtcg aatttggcgt
     4561 tagcccggca ggcgtgtggc gtaaccgatc acacgatccg ctcggttccg atacccgagg
     4621 cgcggcagcc tatgacgaat cctacgcaga cacccgtcga tgggtggaac aaggattgct
     4681 ggattacatt gctccccaaa tttactggcc gttctcacgg agtgccgcgc gttatgacgt
     4741 gttggcaaaa tggtgggcgg atgtcgttaa accaaccagg acccgcctgt atatcggtat
     4801 cgccttctat aaagtgggtg aaccttcaaa gatagagcca gactggatga ttaacggcgg
     4861 cgtaccggaa ctgaaaaagc agctcgatct taacgatgcg gtaccagaaa ttagcggcac
     4921 catcttgttc cgtgaggact atctgaataa accgcagact caacaagcgg tcagctatct
     4981 gcaaagtcgt tggggcagtt aaactaccat gcttactttg taacaagccg gtgctttacc
     5041 caccggcttg ttacaccttg tgaaatatcc cactagtaat catgcttaca taagtcaaat
     5101 taaccacgca attaaccaca cttagttaaa cagctataaa tccctaataa aaatgtatga
     5161 atatgaaaaa ccatacgata ttcgctgtca ccgaaaaata attacgatgg gctatactct
     5221 tctgaagaac agtagcgtag attgaaactg tcttaagtcg cttatttctt ttcagtatat
     5281 cttcatattt caggagaata tggtgcagtt agtcgatctc gcacgttgcg tttcgttttg
     5341 attccgtctg atttcccagc cttataaggg tgtgaattat ggagatgtat tttaaaagaa
     5401 tgaaagatga gtggaccgga cttgtcgaac aggcagatcc tctcattcgt gctaaagccg
     5461 cagaaattgc ccttgcgcat gctcattatc tgagtatcga gttttatcga attgtccgca
     5521 tcgacccgca tgccgaagaa ttcttgagta atgaacaagt tgagcggcag ttgaaaagtg
     5581 cgatggaacg ctggattatt aacgtgcttt ctgcccaggt tgacgatgtc gaaaggctaa
     5641 tacaaatcca gcataccgtc gcggaagtgc atgcccgcat aggaattccg gtagaaattg
     5701 tcgagatggg gtttcgggtg ctgaaaaaaa tcctctatcc ggtaatcttc tcttcggatt
     5761 attccgccgc agaaaaactt caggtctacc atttctcgat taacagtatt gatatcgcga
     5821 tggaagtgat gacccgcgcg tttaccttta gtgacagtag tgcctcaaag gaagatgaaa
     5881 actatcgtat cttctcgtta ctggaaaacg ccgaagaaga aaaagaacgg caaatagcct
     5941 caatactttc atgggaaata gatattatct ataaagtcct gctggattct gatttaggca
     6001 gcagtttgcc tttaagccag gctgattttg gcctgtggtt taaccataaa ggtcgacatt
     6061 attttagcgg cattgccgaa gtgggccata tctcccgtct gattcaggat ttcgacggta
     6121 ttttcaatca aaccatgcgt aacaccagga ttttgaataa cagaagtttg cgggtgaaat
     6181 ttctattaca gataagaaat accgtatcgc agattattac cttgctgcgt gaattgtttg
     6241 aagaagtatc gcgccacgaa gtcggtatgg atgtactgac gaaattactt aaccgccgtt
     6301 tcctgccgac tatcttcaaa cgcgaaattg cccatgccaa ccggaccggt acaccgctgt
     6361 cagtgctgat tattgacgtt gataaattca aagagatcaa cgatacgtgg ggccataaca
     6421 ctggcgatga aattctgcgt aaagtctcat tcctctcgca gaagagattg gtgaaatcga
     6481 aaatattggg cgctgggtca tcgcggaagc ttgccgtcag ttagcagaat ggcgtagcca
     6541 gaatattcat atcccggcgt tatccgtgaa cttgtcggcg ctgcactttc gcagtaatca
     6601 gctgcctaat caggtgtctg atgcaatgca agcctggggt attgacggcc accagctgac
     6661 ggtggaaatc acggaaagca tgatgatgga acacgatacc gaaatcttta agcgcattca
     6721 gatcctgcgt gatatgggcg tgggtttatc ggtagatgat tttggcacgg gcttttccgg
     6781 attatcccgc ttagtcagtc ttccggtaac ggaaatcaaa attgacaaaa gctttgtcga
     6841 tcgttgtctg acggaaaaac gcatcctggc cttacttgaa gccattacca gcattgggca
     6901 aagcctcaat ttaaccgtcg tggcggaagg ggtcgaaacc aaagagcaat ttgagatgct
     6961 acgtaagatc cactgtcgcg ttattcaggg atatttcttt tcccgccccc ttcccgccga
     7021 agaaattcca ggctggatga gcagcgtgtt accgctgaaa atctgacaaa ttcctctcgc
     7081 ccgcactcgc gggttttcct ttaaggtgac actgtcactt aaatactgtg atttcagcta
     7141 cgattccggg agattccttc cttaactttc cctcttgcac caaaactgca ctacgctgca
     7201 caaatgttga tcaaaagtta aaaaatataa ctttaagcca gcattctggc gcttatcccg
     7261 gcatggcatg agatctgcat aagcggaaag cgcagcaatt tttgtcttat acaggaaccg
     7321 tttatgtcgg ataccaccga actggttgat ttagccgtga tcttccctga tctggagatc
     7381 gaattgaaat atgcctgcgt tgataacatc acaggtaaag ctatttatca gcaagcgcgt
     7441 tgtctgctac ataaagatgc gattaccgcg ctggcgaaaa gtatcagtat cgcccagctg
     7501 tcaggcttac aactggtgat ttacgatgcg tatcgcccac aacaagcaca ggcgatgttg
     7561 tggcaagcct gcccagaccc gcaatatgtt gttgatgtga cggtcggttc taatcacagc
     7621 cgtggcacgg cgctcgacct gacgcttcgt gatgacgtgg aggtggtgaa tgatgctaag
     7681 cgaggaaacg tccgccgtac gcccacaaaa acaaacgcga tttaacggtg caaaactggt
     7741 atggatgctg aaaggcagtc cgctcaccgt gaccggcgca gtcatcattg tattaatgcg
     7801 actgatgatg attttttcac cgtggctggc gacgcatgat cccaacgcca ttgatttaac
     7861 cgcccgcctt ttgccgcctt ctgcggcgca ctggtttggc accgatgaag tgggacgcga
     7921 tctgtttagc cgcgtactgg tcggcagtca gcaatcaatt ctcgccggat tagtggtggt
     7981 cgccattgcg ggtatgattg gttcgctact cggatgtcta tccggtgtgc ttggcggacg
     8041 cgcagacgcc atcatcatgc gcatcatgga cattatgctg tcgattcctt cgttggtact
     8101 gacaatggca ctggctgccg ctctcgggcc gagtttattt aacgccatgc tggcgattgc
     8161 tattgtgcga attccctttt atgtgcgctt ggcgcggggg caagcattag ttgtacgcca
     8221 gtatacctat gttcaggcgg cgaaaacctt tggcgcgtct cgttggcatc tgatcaactg
     8281 gcatatttta cgtaactccc taccgccgct gatcgtgcag gcatcgctgg atatcggtag
     8341 cgcgatttta atggccgcca cgttgggatt tattggcctg ggtgctcaac aaccgagtgc
     8401 tgaatggggc gcgatggtag cgaatggtcg caactatgta ctcgatcagt ggtggtattg
     8461 cgcatttccg ggggcagcga ttttgcttac ctccgtcggg tttaatctct ttggtgatgg
     8521 tattcgcgat ctgcttgacc cgaaagcagg aggaaagcag tcatgaccca acccgttctg
     8581 gacattcaac agctgcattt gagtttcccc ggttttaacg gtgatgttca cgcgctcaac
     8641 aatgtgtcct tgcagattaa ccgcggcgaa attgtcggtc tggtgggaga atccggctca
     8701 ggtaaatcag tcaccgcaat gctgattatg cgtctgctac cgacgggcag ttattgcgta
     8761 catcgggggc agatttcact gctgggagaa gatgttctta acgctcggga aaaacagctt
     8821 cgtcagtggc gcggcgcacg agtggcgatg atctttcagg aaccgatgac cgcccttaat
     8881 ccgacacgtc gaataggtct tcagatgatg gacgtaatcc gccatcatca accaataagt
     8941 cgtcgggaag ccagagctaa agcgattgca ctgctggaag agatgcaaat cccggatgcc
     9001 gtggaagtta tgtcgcgcta tccgtttgag ctttcaggtg gtatgcgcca gcgggtaatg
     9061 attgcgccgg cattctcctg cgagccgcaa ttgattattg ccggcgaacc gacgacggcg
     9121 ctggacgtca cagtacagtt gcaggtactg cgtttgctta aacataaagc acgcgccagt
     9181 ggaactgcgg tactgttcat cagccatgat atggccgtgg tgtcgcaact gtgcgatagc
     9241 gtttacgtga tgtatgccgg aagcgtaatt gaaagcggcg ggacggcaga cgttatccat
     9301 catccccggc atccgtatac cattggtttg ctgcaatgcg caccagaaca tggagtacca
     9361 cgccagccat tacccgccat tccagggacg gtaccaaacc tcacccattt gcctgacggc
     9421 tgcgcttttc gcgatcgttg ctatgcggca ggtgcacagt gtgcgctgac agcgtgtggt
     9481 gacaacaacc agcactgcac ctgttggtat ccgcagcagg aggtcataag tgtctgacac
     9541 gttattaacg ttacgcgacg tccatatcaa ttttccggcc cgtaaaaact ggcttggcaa
     9601 atctacggaa catgttcatg ccattaatgg tattgattta cagatccgcc gtggtgaaac
     9661 cttagggatc gtcggcgagt caggctgcgg caaaagcacc ctcgcacagc ttttaatggg
     9721 catgctgcaa ccgagccacg ggcagtacat ccgttcaggc tcacaacgca ttatgcagat
     9781 ggtgtttcag gacccgctct cttcgcttaa tccgcgctta ccggtgtggc gcatcatcac
     9841 agaatcgctc tggatagcta agcgtagtag tgaacaacag cggcgagcgt tggcagagga
     9901 gttggctgtg caggtgggta ttcgtccgga gtatctcgac cgcctgcctc atgcgttctc
     9961 cggcgggcag cggcaacgca tcgccattgc cagagcactc tcttcgcagc ctgacgtgat
    10021 tgtgcttgat gagccaacct cggcgctgga tatctccgtg caggcgcaga tcctcaattt
    10081 gctggtaacg ctacaggaaa atcacgggct gacttatgtg ctgatttcac acaatgtctc
    10141 ggtgatacgt catatgagcg atcgggtggc ggtgatgtat ctcgggcaga ttgtagaact
    10201 gggcgacgcg caacaggtgt tgactgcttc ggcacatcca tacacccgat tattgctgga
    10261 ttccctcccc gccattgata aaccgctgga ggaagaatga gcattacgta aaacggatct
    10321 gccgggaaac cgcacgttgc cgcaaggctg ttttttctac gaacgttgcc ctctggcaac
    10381 ccacggatgt gaagtccggc aatcattagc aataagggag gacggacgtg acctccggtg
    10441 ctggcgggcg ctgtagagat gctaacaacg catcaggcat tcacgcgcct gacgcgtcat
    10501 ccggcaatgc tttacgattt caactggtaa tccagcgtaa tttccgcttt cagtacctga
    10561 gagaccgggc atcctgcttt tgctttctgg atgatgccgt caaaggtaga ggcatcaata
    10621 cccggcaccg caacttcact cttcagtgcg attttcgtaa tcgcaaaacc ggcatccact
    10681 ttatccagcg acacatcggc ggtggtatca attgatgttg gcgtgaatcc cgcttccccc
    10741 agcattaatg aaagcgccat tgagaaacat gcggcatgcg ctgcgccaat cagttcttca
    10801 gggttggttc ctttttcgcc ttcaaaacgc gtgttaaatc catacggctg ttggttcagc
    10861 acgccgctct cggtggatac tgttcccttc ccgcgtttga tatcgccttc ccagtgtgcc
    10921 tgacctttct tatggattgt cattgttgct ctcctgtggg cttaaaaagg aaattataga
    10981 ctacgtgaga aacagcacga ggataagcag gatattccga ataaaatacg gcgaaaatat
    11041 tacttctgca cttaaattaa attcccacaa tagataaccg ggaatatata tctcaag
//
LOCUS       DAETQL010000115        11047 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.115, whole genome shotgun sequence.
ACCESSION   DAETQL010000115 DAETQL010000000
VERSION     DAETQL010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.115"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            410..1240
                     /locus_tag="K0W65_003512"
     CDS             410..1240
                     /locus_tag="K0W65_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBI3096630.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMPYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1381..2154)
                     /gene="uxuR"
                     /locus_tag="K0W65_003513"
     CDS             complement(1381..2154)
                     /gene="uxuR"
                     /locus_tag="K0W65_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBI3096631.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2369..3829)
                     /locus_tag="K0W65_003514"
     CDS             complement(2369..3829)
                     /locus_tag="K0W65_003514"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBI3096632.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3910..5094)
                     /gene="uxuA"
                     /locus_tag="K0W65_003515"
     CDS             complement(3910..5094)
                     /gene="uxuA"
                     /locus_tag="K0W65_003515"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBI3096633.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5434..6777
                     /gene="gntP"
                     /locus_tag="K0W65_003516"
     CDS             5434..6777
                     /gene="gntP"
                     /locus_tag="K0W65_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HBI3096634.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7028..7930)
                     /gene="fimH"
                     /locus_tag="K0W65_003517"
     CDS             complement(7028..7930)
                     /gene="fimH"
                     /locus_tag="K0W65_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HBI3096635.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7950..8453)
                     /gene="fimG"
                     /locus_tag="K0W65_003518"
     CDS             complement(7950..8453)
                     /gene="fimG"
                     /locus_tag="K0W65_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HBI3096636.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8466..8996)
                     /gene="fimF"
                     /locus_tag="K0W65_003519"
     CDS             complement(8466..8996)
                     /gene="fimF"
                     /locus_tag="K0W65_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HBI3096637.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9006..>11047)
                     /gene="fimD"
                     /locus_tag="K0W65_003520"
     CDS             complement(9006..>11047)
                     /gene="fimD"
                     /locus_tag="K0W65_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HBI3096638.1"
                     /translation="IGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKW
                     QHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVI
                     HGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFT
                     VPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLA
                     DRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQ
                     LVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTV
                     TQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRD
                     QMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSY
                     SVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANG
                     VTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTL
                     ADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSS
                     GIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2684 a         2829 c         2682 g         2852 t
ORIGIN      
        1 tacgtagatg aaaatacttg tcaccgctgc tacttaattc cggtgggaaa ttatttattt
       61 ccgcataatc agctatgtat tatttgaaac aattagtttg taaataatat aaattgataa
      121 catatcaaat acatcaaaaa taccattcac aggcattatt aatatagacc tcaatatagc
      181 aatatcaaat tccaattagt aataagatat gcttttaaat tgcatgcaaa taattttaaa
      241 tgagcgtttt ttgatacttt agagtgtttc ctacccttta atatcactgt gtagagttga
      301 tatcactaca acagaaacat gcatgtcatt tgtttacttc tctttttact acagaaaaat
      361 ttatctattt ttctaaacat cagttaaata ctcataaaag gtaactatta tgtctatgcc
      421 attaagcaat gccgctaata accaattcaa tacaaataat cacttccttc atcatccaaa
      481 agtcgacagt gagttaacac gtcgatatga atatgcacgc ctggatacgg aaaatatcta
      541 tcttctgcct cttgctcgtg gcaataacca taattacgat ggaaaaagcg ttgtcgaaat
      601 tagaaaactt aatattggca aacagtcctg gccatttaat tatgtgacag atacatgcag
      661 agaattcaat ggtattacaa caacaggacg tatgccctat cgaaacttga aaatcacctc
      721 agctttagac gaaatatatg gtggtatatg taaaaaggcg catgctgcta cagagttagc
      781 agaaggttta cggctaaatt tatttatgaa gagccctttc gatcctgttg aggactatac
      841 agttcatgaa ataacattag gccctggttg caatgttcct ggttatgccg gaaccacgat
      901 aggatatatt tcaacccttc cagcttctca ggcaaaaaga tggaccaatg agcagccaag
      961 aattgatatc tatattgatc agataataac tgtctcaggg gtagcaaatt cttccggatt
     1021 cgcccttgcc gcattactta atgctaatat tgagatggga aatgacccaa tcattggtat
     1081 agaagcctat cctggtaccg cggagatcca ttcaaaaatg ggatataacg ttatccccgg
     1141 tgatgaagat gcgccactca aacgcatgac tctgcaaccg tcatctcttc cagaactgtt
     1201 tgaattaaaa aatggtgaat ggaattatat tggtaaatag ccactgagaa gaatgagtac
     1261 taatattcag gttatacaga tatttaaaca aaaccctgca atttacaaag taatactcat
     1321 tgcgacttct taatttaaga aagcgcaagg aacgtttacc cttgcgctta ttataataag
     1381 tcaggcgtcg actttatcca gcggccatga atcaaagaga tagccgtcaa aataaatatc
     1441 gtcaacgttc gagaattcca gcagacgttg cttaacgttt tccagatgct gccacattgc
     1501 cagcttcgcc gctcgggcat cttttttgat taacgcggcg aggatctgtt tgtgatcgcc
     1561 caaccactct ttgcgataca ggctgtcatc cagatggctg tgcaactgga tccacattgg
     1621 attgttttcc cgccactgcc aggactgacg gaataactcc accagcatgc tgttatgcgt
     1681 tgcttcagca atggcgagat ggaactgcat gtcaccgctt tcgctgctac ccggcgcgct
     1741 ggaagccagt tcacgctctt ccagttgcaa tgcctgacgc attttgacga tatcttcgcg
     1801 ggtagcctgc agtgcggcaa actcggcgat gttactttcc agtaactgac gcgcctgtaa
     1861 cagctcaaaa ggacccgcat cattgcagac gttggcatcc ggactgtctg tgttgtggct
     1921 gcctgagcta tcaagcacat agataccggc accccggcgc acttccacca gccctttgat
     1981 ctccagcatg atcagcgctt cacgtaccac cgtccgcgtg acatcaagca tttctgcaat
     2041 ttcacgctcc ggcggcagcc gttcgccagg attgtacggc gtctttatga tcagatcgtg
     2101 gatcatcgcc ccgacttcct ggtaaggtct ttgcgcagag gtgacagatt tcatggtgtt
     2161 gtgtccgtta ggtgcggtta atcaaatttt tgccggatgc ggcgtgaacg ccttatccgg
     2221 cctacaacgg gcaattttac ggcccccgtt aatctcaaac tataccatta gcactggaag
     2281 ttggcacgta caggtgggca aaaaaacgaa aactggtcaa ccaaatatca gtttgattga
     2341 ccagtaagtc tgtagggtaa tcagttagtt acagcgcagc cacacactcg cgcgcaccgc
     2401 gttcgcacag ctgctgatat gccgacgtca ccgcgccaac aaagtcggca ttctgcggca
     2461 gatcatcggc aaaaataccg ctcaggccca gcagcgcttt cacgcggtct gcgccctgat
     2521 actgcgcgtt aatcttctgg aactcggcca gcatcgggtc gaccacgtca atggcattac
     2581 cctgctcatc cacgccctgg gtgtaaccca tccagccagc cacgcccagc gccaggtgac
     2641 gccagctgcc gccgttttgc aggtgcagac gcaccgggtc cagtagacgc tgcggtaact
     2701 tctggctgcc gtccatcgca atctgccagg tacggtgacg cagagacggg ttgctgaaac
     2761 gctcgatcag cagcgtcgca taggcgttca ggtccgtacc ttccggcatc gacagcgttg
     2821 gcgcttgttc ctgcatcatc agggcaaagg ccgctttgcg ataatccggg ttagtcatgg
     2881 tgtcggcaat ggtttcatag ccgccgaggt aaccgaggta cgccaggaaa gagtggctgc
     2941 cgttcagcat acgcagcttc atcatttcga acggcacaac gtctgcaacg aactgtgtgc
     3001 ccactttatc ccagtccggg cgaccattaa cgaagttatc ttcaatcacc cactgacgga
     3061 acggttcgca ggcaatggcg cacgggtcgt aaacacccag ctggtcagca atttcctgta
     3121 aggtttctgg cgtcgccgcc ggaacgatgc ggtcaaccat ggtgcacggg aaggtgacgt
     3181 tctcttcaat ccatgccgct agctgcgggt cacgcgcctg agccagcccc agtaccgcga
     3241 ccttcgccac atggccgttt tcacgcacgt tatcgcagga catcaccgta aatgctttca
     3301 gccctttttc acgacgcaga cgcaaggctt cgacgatgta accaatcgcg gacttcggcg
     3361 cagtcgggtt ttccagatcg tgcttgatca gcgggttatt gagatccagt tgaccgcttg
     3421 ccgcatcagc gcagtagcct ttttccgtga ccgttagaga gacaatcgcc gtttgcggac
     3481 gcgccatcgc gttgagaata ccttcgcagc cgtcgatttc cggatgcagc gcttctttca
     3541 tcgaaccgat aattttcagc tcggtgcttt ctgcgccttt ttccgctacg gtgtacagca
     3601 gttgctgttt tttcaggttt tcgatcagca tgcggtcgtt gcctggcatc aggttaactt
     3661 cgcagatgcc ccagtcgctg tcggtgcttt ccagcagatg atgggtatac agcgcctggt
     3721 gcgcgcggtg aaacgccccg caaccgagat gcacaatgcg tgattccaga cgagaatgat
     3781 cccatgacgg gcgggcaacc ggcagattgc tgtcaacaat agtagtcatc gtaaactcca
     3841 gaatccgtct tcagagagtg acggcaagga agaaggaacc ggcacggcgc gcagccatgc
     3901 cggtgggtat taacggctaa agaaagcgcg ctggatcgcc agttcgacac cgcgaacttc
     3961 ggccaggcct ttcagacgac caattgcgga gtaaccaggg ttggttttct tcttcaggtc
     4021 gtccagcatc tgatgaccgt ggtccggacg catcgggatc aggtcttctt tgccttccgc
     4081 tttacgacgg tgttcttctt caacaatcgc tttcaccact ttgtacatat caacgtcacc
     4141 gttcaggtgc gccgcttcgt ggaaggtttt cgggttatct tcacgcatgg tggagcgcag
     4201 atgggtgaag taaatacgcg gaccgaactg cttgatcata tcaaccagat cgttgtcagc
     4261 acgcacgccg taggaaccgg tgcacatggt gaaaccgttc gccatgctgt ttacggtatc
     4321 aaccatccac tgcatatctt caatggtgga aacaatgcgc ggcaagccga ggatcgggcg
     4381 cggcggatcg tccgggtgaa ccgccatacg cacgccaact tcttcagcaa ctggaataat
     4441 cgctttcagg aagacggcaa agttttcgcg cagtttggct ttgtcgatat ctttgtacag
     4501 ctccaggtgc ttacggaact ggtcgagggt atacccttct tccgcgcccg gcagaccggc
     4561 aatgatgtta cgcgtcagac gcgctttgtc ttcgtcgctc atagtggcga agcgttcagc
     4621 tgcctgagca atttcttctt cggtgtaatc cgcttccgcg cctggacgtt tcaggatatg
     4681 catttcgaat gcagcgaatt cgatctggtc gaagcgcaga gctttggagc cgtctggcag
     4741 cacgtattcg aggtcagtac gggtccagtc gagcaccggc atgaagttgt agcacacggt
     4801 gcgaatgcca cactgcgcca ggttgcgcag agtctgctga tagttagcga tccactgctc
     4861 atagttgcca gtgtgggttt tgatatcttc gtgaattggt acgctttcta cgacagacca
     4921 caccaggcct gcgtcttcaa cgatcgcctt gcgtttgagg atctcttcta cggaccatac
     4981 ttcgccgttc gggatatggt gcagcgcggt aaccacgcca gttgcgcccg cctgacggac
     5041 atcagctaaa gaaaccggat cgtttgggcc gtaccagcgc caggtctgtt ccatgatttt
     5101 tcctctttca catagtgggt tccacagcaa agagacgcgg aaccttggag ttgtttaggt
     5161 atccggatga acaaccagac cgcagccgtg gcgttgcatc ggatgagatg gcgtataagt
     5221 tctacctttt ggtaagacat gtcaaccaga attctgaaat tggttaaccg cgtcgcaaga
     5281 atttcactct catggttgtt gctgcaaatt gcagcaaagg gtaatcgatg gttattgatc
     5341 agaggatgtg acattcatcg caacaatggt tgaccaattt acataacatg tcgcccaaaa
     5401 taacaacggt tcaaccaagt aaggtaaaac gttatgcatg tgcttaacat tctctgggtg
     5461 gtattcggca ttggcctgat gctgatactg aatttgaagt tcaaaatcaa ttcaatggtg
     5521 gctttgttgg tggcggcgct gtccgtcggg atgctggcag gcatggattt gatgtcgctg
     5581 ctgcacacca tgaaagcggg cttcggcaac acgctgggag aactggctat catcgtggtg
     5641 ttcggtgcgg tcatcggtaa attgatggtc gactccggcg cggctcacca gatagcacat
     5701 acgctgctgg cgcgtctcgg tctgcgctat gtacagctgt cggtgattat catcggcctt
     5761 attttcggtc tggcgatgtt ctatgaagtg gcctttatca tgttagcgcc gctggttatt
     5821 gttattgccg ccgaagctaa aattccgttc ctgaaactgg cgatcccggc agtagcagct
     5881 gccactaccg cacattcact gttcccaccg cagccgggtc cggtggcgct ggtgaatgct
     5941 tatggcgcgg atatggggat ggtttatatc tatggcgtac tggtgacgat cccaagtgta
     6001 atctgcgcag gtctgatcct gccgaagttc ctcggcaatc ttgagcgccc aacgccatca
     6061 ttcctgaaag cagatcaacc ggtagatatg aataatctgc cctctttcgg cgtttcgatt
     6121 ctggtgccgc tgatcccggc gatcattatg atctctacca ccatcgccaa tatctggctg
     6181 gtaaaagata cccctgcctg ggaagtggtt aactttattg gttcctcgcc gattgcgatg
     6241 tttattgcga tggtggttgc attcgtactc tttggcaccg cgcgtggtca tgacatgcag
     6301 tgggtgatga acgcttttga aagcgcggtg aagagtattg caatggtgat tctgatcatc
     6361 ggtgcgggtg gcgtgctgaa gcagaccatc atcgacaccg gcattggcga caccatcggc
     6421 atgttgatgt cccacggcaa tatctcgccc tacattatgg catggctgat cactgtgcta
     6481 attcgtctgg cgacgggtca gggtgtcgtt tcggcgatga ccgccgccgg gattatcagt
     6541 gctgcaatcc ttgatccagc aaccggtcag ctggttggcg tgaatccggc gctgctggta
     6601 ctggcgacgg ctgcgggttc caacaccctc acccacatta atgatgcatc tttctggttg
     6661 ttcaaaggtt actttgacct gtcagtaaaa gacacgttga aaacctgggg tctgctggag
     6721 ctggtcaact ccgtggttgg gctgattatt gtgctgatta ttagcatggt agcgtaaaag
     6781 aacaccagag cctgccaatg gcaggctcag actgatgact aaagtctaaa aatgtgcccg
     6841 gacagtcccc tcgcccctcc ggggagaggg ttagggtgag gggaacagac cagtaaagtg
     6901 cgctttgttt atgccagatg cgacgctgac gcgtcttatc tggcctacaa agggctaacg
     6961 tgcaggtttt gtaggtctga tagcgtagcg cctcaggtac cagcattagc aatgcgctgt
     7021 aattttttta ttgataaaca aaagtcacgc caataatcga ttgcacattc cctgcagtca
     7081 cctgccctcc ggtacgtgcg taatttgccg ttaatcccag acttaccgcc gaagttccta
     7141 ctgctcctaa cgataccgtg ttattcgctg gaataatcgt accgttgcgc gtcaactgta
     7201 cgccgacgcc ctgcgctggt gaaaacgacg cggtattggt gaaaatcgag ttgcccgcat
     7261 ctgcggttgt gccggagagg taatacccca ggttttggct tttcgcacaa taaacggtaa
     7321 gaggaattgg cactgaacca gggtagtccg gcagagtaac ggtgacatca cgagcagaaa
     7381 catcacaacc gccagtgggc accaccacat cattattggc gtaaatattc cacacaaact
     7441 gaaaatcatc gctgttatag ttgttggtct gtcgcaaaat aagcacggca attaatgaac
     7501 cagctttaat agccaccccg cccgcactgc tcacaggcgt caaataaagc gccaccggcc
     7561 acggcttatc cgttctcgaa ttataaacaa cccgcggcgt ttcgctggtg gtcggaaatg
     7621 gatagctact gccactatat tttacggtcc cggaaaaatt agataacacg ccgccgtaag
     7681 ccgagcctcg ttgcagtgtg acatagtctg taatggtttc cggataatcg ttatggcaaa
     7741 agatttgcgt cgaaagatct acgaccaggt tttgccccac attcacgaca ggcgcaaggt
     7801 ttacataaac attagcgctg ccaccgccaa tagggatagc ggtaccattg gcggttttac
     7861 aggcgaatga ccaggcattt accgaccagc ccatcagcag tacagcaaac agggtaatag
     7921 ctcgtttcat tacaattatc tcttcgggtt cagctgtagg tataggtgat gctaatcact
     7981 gcctgaatgg ttccctgagt ggctccgcca tttaccgtca atgctctgac ctgtaacggg
     8041 aagtgcgctg attgtgagga atcatccacc tgaactgttt tggttgcgcc agtattcaat
     8101 gtgttgccac tgtcatcctg tagctctaac tggatgtttt gcgcggtccc ctggttttta
     8161 taatatccgg tgctgtcggc tgccccgctg aagctggcag tgaccctcga cgttcccacc
     8221 ggacaattag tcaactcaag cgcaacatca tgccaggccg atgccgcccc ggcagacata
     8281 agactgaaag aataaagatc gccgagatct accgtggcat tggtggtgga aaccgtacac
     8341 ggtttggcga cgaccttacc gttcaccgtg atggtgacat cggctgcctg tatcgtcgca
     8401 cttgcgagcg ccaacattgc cgccaataca tacccacgtt tgcaccattt catgagcatc
     8461 tccagttact gatattcaag agtgaaggta gccgtagcat tgatatgccc cgcagtgaca
     8521 ggcacctgtg tcgccattag ccgggcgtaa aaattcagcg tatttggttt acccggcgtc
     8581 agggtcgtcc acgaaatcgc agacgatgga gcattaaggg gtatttgatt ttgctgctca
     8641 ttcagaagct gtattcccag tcccgcagcc gctgacaccg tattttcaag tgcaagcagg
     8701 ttggcattgt ggctatctgc aacgccggta aacccaactt ttacggcaga aacggcatta
     8761 ccacagggtg acagcaaaat acgaaatgga acgacaggag tcgtcgcgcc aatgttgtta
     8821 aattgcttcg ccgcgttttc catcagatca acagtaaaat tggttgattc agcggccaca
     8881 ctacagccgt tatctctgac atagccgcgg atagtaatcg tgctatccgc agccaaaacg
     8941 cgacttaccg ccagccacaa aaaagcgcac agaagataaa aaggtttgtt tctcatcacg
     9001 cccccttaac gacattcagc tgatagctgg gttaataact gctgctgact ctctggtggc
     9061 agttgataat tggcgacaca gtgagcattt tcctcttctc cccatttcac ctgaactttt
     9121 cccgctaaag gcattccgct gaggtaaacc tgaccattat ccgcaacaat gccgctactc
     9181 tggctactct ctgatgtcac catcgcccca aacggcagcg gcttattatt gtgagttagc
     9241 gtcatgagca gttttatccc aacgcgcgct ttaaactctg ctcgcacgat cgccccacga
     9301 gtgggaacaa cgttagcgac cgcgttatct aaatcgacgt tatcagccaa ggtattggta
     9361 tccagcgcca ctctattttc ccgatattca gtggcataag gcagcacggc ataaccacgc
     9421 cagtcggtac gcacccccgt ctggttttcg acttttgcat cttttgcgcc aggcgcttta
     9481 acaagcacca ccgtatcgtt taacggctgc cccagcgtta cgccattggc atgagccaat
     9541 accccaccgc tgactccgta atagagctgc ttaatatcat cgctatggct gtaaccgata
     9601 ttggcattgc cgtaaccacc gcgataattc agcgtggcgt agcctgtact tccgctatta
     9661 ccatcgcctc ccccggcata gccggtttgc acgctataac tgaggttgtt gtcttccagc
     9721 aacgtaccgt atacaccagc cagattggtc atccgaccgt tgagatcgtg tgacatgctg
     9781 tagctggcac tggcatgtcg ccactgagat ttactgtcag aacgcagcca gtggctgaaa
     9841 ggaatattga cgttaagcgc taacatctga tcccgtcctt tttgccaggc gtttttcgtc
     9901 aggctatagc tgagcgtcca gttgatatct tcgaacgcag tatttaatcc agcctggaat
     9961 tgctcatcga cattactcgt tccccaataa gtttgatggc taccactcaa atacagtgtt
    10021 gatgtgcgcc cgagttgctg agtaacggtg agctgtaatt tcccgcgttt gttataagcg
    10081 aggttgtaat agtcggtgaa tttcggctta acctgaataa ctccatcctg tgtttcgatg
    10141 ttgtagccat tcattcgact gtatgttgta tcagcgaaat taaaatatcc gctggtcgaa
    10201 taacggtaac ccactaactg aatattcgtg cctgattcat tgagcgattt gttatagaga
    10261 aaacgcaccg attgtccgtc atgctgactg tcatcgggaa gtgtggaatt agcctgcgtc
    10321 atatccacag acagagcgcc cagtgcctcc atgtttttcc cgataccgaa attaaaagca
    10381 cgataacgat ccgccagttg cgttccacca tatattgtcc agccagccgg aaggccgtgg
    10441 agtaatgtac tctggaaaaa gcggggtttt tcctgctgcg catttccact acggtattct
    10501 cctgccgtaa tggaataacg agtatgccct tcacgttgca aaagcgggac tgacgaatag
    10561 ggtacggtaa aaatctgcgt gctgccgtca gcctctttga tcgttacctg caagtcacca
    10621 ctattacctg cggcatagat atcgttgatg gtaaaaggcc ccggtggcac cgtactatta
    10681 taaatgccat atccattttg tttaatagtg acctgtgcag taccacgagc aataccgtgg
    10741 atcaccgggg caaatcctct ttggctatcg ggtaacatat tgtcatctga ggccaattgt
    10801 gcgccgcgaa agttaatacc atcgaaaata tcgccctggg tataaccatc acccaacgtc
    10861 agccgggaac gtaaaggaat tatgtctcgc tcaatccagg taatgatatg ctgccattta
    10921 tttttgctac ctgatgaact gtcgctactg ttataactcc aggtggtatt gtcgcgtaaa
    10981 cgccacgcac caatatttaa cccactctgt aggtttaaat atgcataatg gctgttaccc
    11041 ccaatcc
//
LOCUS       DAETQL010000116        11036 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.116, whole genome shotgun sequence.
ACCESSION   DAETQL010000116 DAETQL010000000
VERSION     DAETQL010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11036
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.116"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            154..504
                     /locus_tag="K0W65_003521"
     CDS             154..504
                     /locus_tag="K0W65_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBI3096639.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            557..952
                     /locus_tag="K0W65_003522"
     CDS             557..952
                     /locus_tag="K0W65_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HBI3096640.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            993..1736
                     /gene="cmoA"
                     /locus_tag="K0W65_003523"
     CDS             993..1736
                     /gene="cmoA"
                     /locus_tag="K0W65_003523"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBI3096641.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1733..2704
                     /gene="cmoB"
                     /locus_tag="K0W65_003524"
     CDS             1733..2704
                     /gene="cmoB"
                     /locus_tag="K0W65_003524"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBI3096642.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="K0W65_003525"
     CDS             complement(2869..5298)
                     /gene="torZ"
                     /locus_tag="K0W65_003525"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HBI3096643.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5323..6423)
                     /locus_tag="K0W65_003526"
     CDS             complement(5323..6423)
                     /locus_tag="K0W65_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HBI3096644.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="K0W65_003527"
     CDS             complement(6811..7557)
                     /gene="cutC"
                     /locus_tag="K0W65_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBI3096645.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7571..8137)
                     /locus_tag="K0W65_003528"
     CDS             complement(7571..8137)
                     /locus_tag="K0W65_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBI3096646.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8353..10086
                     /gene="argS"
                     /locus_tag="K0W65_003529"
     CDS             8353..10086
                     /gene="argS"
                     /locus_tag="K0W65_003529"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBI3096647.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10263..10751
                     /locus_tag="K0W65_003530"
     CDS             10263..10751
                     /locus_tag="K0W65_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HBI3096648.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(10869..10937)
                     /locus_tag="K0W65_003531"
                     /pseudo
     CDS             complement(10869..10937)
                     /locus_tag="K0W65_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
BASE COUNT         2690 a         2815 c         2634 g         2897 t
ORIGIN      
        1 taatgggctt ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact
       61 catagctaac cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta
      121 ggaaccatct gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt
      181 catgcatcac gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt
      241 ccggtacatg ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat
      301 atgacgcaaa accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag
      361 accactacgc cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta
      421 cataccctgt gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt
      481 ccacagcaat cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat
      541 tttaaaggga gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat
      601 gaagttctct tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg
      661 tggttttagc gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc
      721 catcgcgatt gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca
      781 tatttgcggc atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg
      841 tctgtttcgc tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt
      901 gctggcgaat ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat
      961 acgccacttt ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg
     1021 cccctatcgc cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg
     1081 atatgatcca gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag
     1141 ccgagcgctt cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg
     1201 cgacgctctc ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca
     1261 actccccggc gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc
     1321 cagtagacgt cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg
     1381 tgctgaattt taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa
     1441 tttatcaagg actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag
     1501 atgccaaagt tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt
     1561 acagcgaact ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt
     1621 ccgtggaaac ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt
     1681 tccagtgctt taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga
     1741 ctttggtaac ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct
     1801 gcccgcgcag attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc
     1861 caacacggtg gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt
     1921 aaccgccgaa agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat
     1981 gcggaacctg atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac
     2041 cgaatggcgt tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg
     2101 acgcaccatt ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc
     2161 aggcgcgcac ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc
     2221 ggtgcgtaaa ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca
     2281 gcttccggca ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg
     2341 ttcgccgctg gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt
     2401 gctggaaacg ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta
     2461 cgcgcaaatg cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa
     2521 gaagtgtggt tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca
     2581 gcgacgcact gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc
     2641 gagtaagaca gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc
     2701 gtaaaggtct ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct
     2761 tgctaattca atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt
     2821 tttacgtttg cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac
     2881 agcaggctta tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac
     2941 cagcgcagag ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa
     3001 cacgttcgca ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg
     3061 cacgcatacc acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc
     3121 acgtttgttc cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc
     3181 ggtgtgaata gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata
     3241 gttaagctga ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc
     3301 gtcggcggta cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt
     3361 atagccaaat ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag
     3421 cgcatttttc accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc
     3481 gctgcggcgc atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt
     3541 gacgcgttgc gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat
     3601 ttcatcttta ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc
     3661 ggcaaacacg tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg
     3721 cacaatatgc tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt
     3781 ggtgatcggt aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac
     3841 gacgatcatc tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt
     3901 aaagttaccg ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc
     3961 gttatgctga tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat
     4021 cgccgtcatt ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat
     4081 ttccggcagc acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa
     4141 accaaaaccg ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat
     4201 ccagtgtttc tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt
     4261 gcgattcgcc gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat
     4321 ttccgccgtc cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc
     4381 aaactgcgga tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt
     4441 catcagggta tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat
     4501 ccaggtggca ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat
     4561 cactggtttg ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact
     4621 gctccaggca atttttaagg tattcagcgg gttcattccc cacagcacca caacctggct
     4681 gttttccaga atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac
     4741 atgcggcatg atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc
     4801 gcccgccagg ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga
     4861 gcgccagccg taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc
     4921 atgttgctca tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc
     4981 gccacgacct ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg
     5041 ctgaatacgc gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat
     5101 ggttttcgcc agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat
     5161 cgctccccaa cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag
     5221 cggtgctgcc gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc
     5281 acgtcttgtt aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat
     5341 atctttggcg ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct
     5401 ggttcgtgcc cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc
     5461 ggcaatagag gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca
     5521 tagcggttta cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg
     5581 ccattgattg ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc
     5641 ttcttcactt aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc
     5701 actgccctgc atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac
     5761 gacggtgaga tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa
     5821 aggataaata tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc
     5881 cgcgttgtca tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat
     5941 acaggtttcg ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga
     6001 ggcaatatcc atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt
     6061 taattctttc catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact
     6121 gtctattttg ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa
     6181 taaataatcc atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt
     6241 ctggttcgaa aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca
     6301 ggaaaggcaa aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc
     6361 gccgccacca accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg
     6421 catagattcc tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac
     6481 gaattaaccg cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga
     6541 tttattacaa tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata
     6601 tttatcagtt ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc
     6661 acaacgctat gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac
     6721 tgctcaggga taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc
     6781 atattgggcg acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc
     6841 tttcatttca gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc
     6901 atctgatgac atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc
     6961 cgcggagcta tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg
     7021 gactcctgct ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt
     7081 tgataaacct tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa
     7141 ttccgcaaga ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa
     7201 agtcactgcc agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt
     7261 cccgtcaaca tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg
     7321 cacatcctca agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg
     7381 gcgaattatc ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa
     7441 cgacggcgtt aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt
     7501 ttgctgcgcc gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt
     7561 tgctccttaa ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa
     7621 acttaatagt tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct
     7681 caccttttgg tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa
     7741 gcaatgccag cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat
     7801 gttcccaacc ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa
     7861 actgccagtg cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat
     7921 tgatcatttt ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag
     7981 cggtgacgtt ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca
     8041 accccagacg gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa
     8101 tatcctgcag ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa
     8161 aagcgttaat ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga
     8221 tgcaatagtg aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca
     8281 gtatactccc gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg
     8341 taaggtattc cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt
     8401 gcggcaggcg cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc
     8461 ggcgactatc aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga
     8521 caattagcag agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag
     8581 atcgccggtc caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt
     8641 cagcaggcgc tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg
     8701 gttgactact ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc
     8761 attattggtg acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca
     8821 aaccacgtcg gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag
     8881 cagcaggaaa acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg
     8941 aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa
     9001 ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg
     9061 acgcagaacc agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg
     9121 ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt
     9181 ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgtgtttaa aaacaaggaa
     9241 ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact
     9301 gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac
     9361 atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc
     9421 tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac
     9481 ggcaaaccgt tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa
     9541 gccctggagc gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag
     9601 ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa
     9661 aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc
     9721 gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt
     9781 aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg
     9841 gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg
     9901 catgtaatgt gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac
     9961 tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa
    10021 ctgacagcga agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt
    10081 atgtaatcga tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta
    10141 ttgccatcta aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga
    10201 ggccagaagc gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg
    10261 aagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag
    10321 atacggttat agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt
    10381 cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg
    10441 tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac
    10501 gcacaggtgc aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag
    10561 cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg
    10621 atttaagtga cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata
    10681 cgcgaactcg ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga
    10741 caagaggata attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat
    10801 tgcaacaaaa catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt
    10861 tttgtctttc agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt
    10921 aaccgccctg aaccgccccg ggtttcctgg agagtgtttt atctgtgaac tcaggctgcc
    10981 agatcatcgt ttccgatgga agcataataa gctttttctg cttctgccgg aggagt
//
LOCUS       DAETQL010000117        10962 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.117, whole genome shotgun sequence.
ACCESSION   DAETQL010000117 DAETQL010000000
VERSION     DAETQL010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10962
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.117"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..203
                     /locus_tag="K0W65_003532"
     CDS             <1..203
                     /locus_tag="K0W65_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096649.1"
                     /translation="GASYRYSGAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSIAI
                     YLLAGCLISAMTALLMKDNQRS"
     gene            305..1759
                     /gene="amn"
                     /locus_tag="K0W65_003533"
     CDS             305..1759
                     /gene="amn"
                     /locus_tag="K0W65_003533"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBI3096650.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2102..2818
                     /locus_tag="K0W65_003534"
     CDS             2102..2818
                     /locus_tag="K0W65_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBI3096651.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3274..3349)
                     /locus_tag="K0W65_003535"
     tRNA            complement(3274..3349)
                     /locus_tag="K0W65_003535"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3314..3316),aa:Asn,seq:gtt)
     gene            complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="K0W65_003536"
                     /pseudo
     CDS             complement(3450..5077)
                     /gene="yeeO"
                     /locus_tag="K0W65_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5082..5157
                     /locus_tag="K0W65_003537"
     tRNA            5082..5157
                     /locus_tag="K0W65_003537"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5115..5117,aa:Asn,seq:gtt)
     gene            complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="K0W65_003538"
     CDS             complement(5195..6145)
                     /gene="cbl"
                     /locus_tag="K0W65_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBI3096652.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6247..7164)
                     /gene="nac"
                     /locus_tag="K0W65_003539"
     CDS             complement(6247..7164)
                     /gene="nac"
                     /locus_tag="K0W65_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HBI3096653.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7492..7567
                     /locus_tag="K0W65_003540"
     tRNA            7492..7567
                     /locus_tag="K0W65_003540"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7525..7527,aa:Asn,seq:gtt)
     gene            complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="K0W65_003541"
     CDS             complement(7623..8558)
                     /gene="ldtA"
                     /locus_tag="K0W65_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBI3096654.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8620..9699)
                     /gene="cobT"
                     /locus_tag="K0W65_003542"
     CDS             complement(8620..9699)
                     /gene="cobT"
                     /locus_tag="K0W65_003542"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBI3096655.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9711..10454)
                     /gene="cobS"
                     /locus_tag="K0W65_003543"
     CDS             complement(9711..10454)
                     /gene="cobS"
                     /locus_tag="K0W65_003543"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBI3096656.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10451..10960)
                     /gene="cobU"
                     /locus_tag="K0W65_003544"
     CDS             complement(10451..10960)
                     /gene="cobU"
                     /locus_tag="K0W65_003544"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBI3096657.1"
                     /translation="MVVSAIASTPHSSQVLYIATSQILDDEMAARIEHHRQSRPEHWR
                     TVERWQHLDELIHADINPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINA
                     EIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLV
                     VSGIGVKIK"
BASE COUNT         3046 a         2785 c         2376 g         2755 t
ORIGIN      
        1 ttggtgccag ttatcgctac agtggtgctg gagtcggtta tcaggttgcc agtgtggttg
       61 gcggtggatt tacacctttt attgccgctg cactcatcac ttactttgcc gggaactggc
      121 atagcatcgc catttatttg ctggctggat gcctgatttc tgcaatgacc gctttattga
      181 tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca tccgatcttc gccttatact
      241 tttgtttcac attcctgtga catactatcg gatgtgcggt aattgtatgg aacaggagac
      301 acacatgaat aataagggct ccggtctgac cccagctcag gcactggata aactcgacgc
      361 gctgtatgag caatctgtag ttgctttacg caacgccatt ggcaaatata ttacaattgg
      421 cgaattacct gatgaaaacg cccgtaaaca aggtcttttt gtctatccat cactgaccgt
      481 aacctgggac ggtagcacaa caaatccccc caaaacgcgc gcatttggtc gttttactca
      541 cgcaggcagc tacaccacca cgattactcg ccctactctc tttcgttcgt atctgaatga
      601 gcaactaacg ttgctttatc aggattatgg tgcgcatatc tctgtgcaac cctcgcaaca
      661 tgaaatccct tatccttatg tcatcgatgg ctctgaattg acacttgatc gctcaatgag
      721 cgctgattta actcgctact tcccgacaac agaactggcg caaatcggcg atgaaactgc
      781 agacggcatt tatcatccaa cggaattctc cccgctatcg cattttgatg cgcgccgcgt
      841 cgatttttcc ctcgcacggt tgcgccatta taccggtacg ccagtagaac attttcaacc
      901 gttcgtcttg tttaccaact acacacgtta tgtggatgaa ttcgttcgtt ggggatgcag
      961 ccagatcctc gatcctgata gtccctacat tgccctttct tgtgctggcg ggaactggat
     1021 cactgccgaa accgaagcgc cagaagaagc catttccgac cttgcatgga aaaaacacca
     1081 gatgccagca tggcatttaa ttaccgctga tggtcagggt attactctgg tgaatattgg
     1141 cgtgggaccg tccaatgcta aaaccatctg cgatcatctg gcagtgctac gcccggatat
     1201 ctggttgatg attggtcact gtggcggatt acgtgaaagc caggccattg gagattatgt
     1261 ccttgcacac gcttatttac gcgatgacca cgttcttgat gcggttctgc cgcccgatat
     1321 acctattccg agcattgctg aagtgcaacg tgcgctttat gacgctacca agttagtcag
     1381 cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca ggtactgtgg taaccacaga
     1441 tgacaggaac tgggagttac gttactcagc ttctgcactt cgttttaact taagccgggc
     1501 cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa ggatatcgtt tccgcgtgcc
     1561 atacgggaca ctactgtgtg tttcagataa accgttacat ggtgagatta agcttcccgg
     1621 ccaggctaac cgtttttatg aaggcgctat ttccgaacac ctgcaaattg gcattcgggc
     1681 gatcgatttg ctgcgcgcag aaggcgaccg actgcattca cgtaaattac gaacctttaa
     1741 tgagccgccg ttccgataat aagttatgaa ccttcttatc agaaatagat gtgaaggatt
     1801 aagtaagaca tctggcaaat agcctgcaat aaacgcgggc tgcttgcttc cttgggcaga
     1861 tgcgagtttt attatcgtct taatgatttc cacatattaa aagcaagtat gctttcaaaa
     1921 cacaattata aaaaatcccg ccaacaacat tagtttttat aaaattaaat ataagattat
     1981 ggctttagaa tatttttatt tctaatagac gagatttttc ctgttatgat ataatatgcc
     2041 gaattaacac atgttaacga tttaccagta atgtaaataa attttcgagg agatcattcc
     2101 agtgggacgt aaatgggcca atattgttgc taaaaaaacg gctaaagacg gtgcaacgtc
     2161 taaaatttat gcaaaattcg gtgtagaaat ctatgctgct gccaaacaag gtgaacccga
     2221 tccagaatta aacacatctt taaaattcgt tattgaacgt gcaaagcagg cacaagttcc
     2281 aaagcacgtt attgataaag caattgataa agccaaaggc ggcggagatg aaacgttcgt
     2341 acagggacgt tatgaaggct ttggtcctaa tggctcaatg attattgctg agacattgac
     2401 ttcaaatgtt aaccgtacga ttgctaacgt tcgcacaatt ttcaataaaa aaggcggcaa
     2461 tatcggagcg gcaggttctg tcagctatat gtttgacaat acgggtgtga ttgtatttaa
     2521 aggaacagac cctgaccata tttttgaaat tttgcttgaa gctgaagttg atgttcgtga
     2581 tgtaactgaa gaagaaggta atattgttat ttatactgaa cctgctgacc ttcataaagg
     2641 aatcgcggcg ctaaaagcag ctggaatcac tgagttctca acaacagaat tagaaatgat
     2701 tgctcaatct gaagttgagc tttccccaga agatttagaa atctttgaag ggcttgttga
     2761 tgcccttgaa gatgacgacg atgtacaaaa agtttatcat aacgtcgcaa atctctaatt
     2821 atattttaaa gaaatctgtc tttatggcag atttctttaa tctcatataa ttcttataaa
     2881 aaatataata tttaactcgt catattgctt ataaaaaatc cccgtcccca gagaaataat
     2941 atttattaaa atggcaggtc ttctttttct gattaccgaa gacaaagtgg caattacgta
     3001 tagtttcccg ataaagacgc gacagctacg tcccgcataa ggcatttttc tctttatctt
     3061 tgtacggtac ttcatggaac agaatttttg accttgtgaa tcgtgatatc tgttggggag
     3121 gggcaatttg ctcactgaag cgtgagactc gattaagcgc acgaaacaca gaaatcaaaa
     3181 aacccggcca cttttttaca aagtggccgg gtaaaaataa tttttatttt ttaactgtcc
     3241 tgagtctcat agagatgtct caaaactaaa atttggctcc tctgactgga ctcgaaccag
     3301 tgacatacgg attaacagtc cgccgttcta ccgactgaac tacagaggaa tcgtgagaac
     3361 gaggcgaata ttagcgatgc ccacccacaa tgtcaaagcc tgttttttaa atttgaaatc
     3421 gtttgctgaa ataatctgca ttttgtcgtt tattccgaca caactggctt tttttcacac
     3481 ttttgcggct cggatcgagg gtatttccac agccaacgtc cagtaaccat tcgccagtaa
     3541 aacagcacgg cccgcacagc ccagtcggca aacattccca tccagacacc aaccacaccc
     3601 cagccaagca tgattccctg cacataaccg actacaaccc ggcaacccca catgctcaac
     3661 atagaaaccc acatggcgta acgggcatca cgagcaccct taaatccagc gggtagcacc
     3721 catgaggcgg accaaatagg cataaataaa gcatttagct aaatcagaat cacaacgacg
     3781 tgtttaacct gtggatcctg ggtgtaaaac gatgccataa ccccggcaaa gggagccgtt
     3841 agccaggcga tggccgttaa tccaagagtg gacagccaga acacatgccg caactgaatc
     3901 tctgcttgcg ctatctgccc tacccccaac cttcggcctg taatgatcgt agaagcagaa
     3961 ccgagcgcac ttccgggtaa gttgataaga gccgcaattg agaacgcgat aaaatttccg
     4021 gcaataacgc tggtccccat cccggcaacg aacatttggg ttaataaccg accactggta
     4081 aataacactg attcgacact cgctggaata ccaatcccca tgacttccca gataatgcta
     4141 aaattcagcg gtttaaaata gctctttaac gaaatcctta gcgcaggatt aaaaccaatc
     4201 gccagcaccc acaaaattgc aactgcgcca atataacgag aaatggttaa acccaaccca
     4261 gccccgacca ggagaaaagg ccgtaaatca atatgccgct aataataata ttaagaatat
     4321 tcaggctacc gttaatcagt agcggtattt tcgtattccc tgcaccacga agtgccccgc
     4381 taccaataag agtgatggca gcggctggat aactgagtac ggtcagctcc agataagtca
     4441 acgccagtgc tttaacttct gtcgtggcat cacccgcgac gaaatcaata atttgttcgc
     4501 caaaatgatg aattagcgtt gccaacagta cggcaaacaa cgtcatgatc accaatgact
     4561 gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc gagactaaat gctacaacga
     4621 cagtagtacc aagatcgata gctgcaaaaa aagccataat gaccatattg aagctgtccg
     4681 ccaaccccac gccggccatc gcatcttttc ccagccaact gaccagaaaa gtgctcagga
     4741 cccccatcaa caggacacag gcgttctcca tgaagatagg aacagcaagc ggggttatct
     4801 cgcgctagaa caacactttg tagctcttgc gtttagagtg ccagcgagtg ccgtgaacaa
     4861 cctggcgtaa agcagaggag atattcaaag ccgaccttaa ttgcagaaag tgaaaccaca
     4921 tttcaaatga tgagggagaa tcagcaaagc tgcaaagatt ttcgccaaca aattgtctgc
     4981 aaatgcaaca aactgttgat agaaacggca aacagttggg aaatttaaaa atcgggtttg
     5041 acaaaatatt tttcgccgtt aagatgtgcc tcaacagcga ttcctctgta gttcagtcgg
     5101 tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat
     5161 ttctgaaaag cccgctttta tagcgggctt tttgctatat ctgataatca atttcctctt
     5221 cactgttttc catcacctgg cgcttgatat cctcaactga cagtcctgca ttacaaagtt
     5281 ccagaaagcg ccaaacatag ttacgctgaa gttgtcctcg cttcagtccc aaccagacag
     5341 tattagcatc gaaaagatgc cgcgtatcca ggcggattaa attctcttcc tcttgttcgc
     5401 cgctggattg ttcggcaact aatccgatcc caagcccaag agcaacatag gttttaatga
     5461 catcagaatc ctgtgcactt aatacaatat ctgccggcaa acctttgcgg gtaaatgcgt
     5521 catcagtgcg tgagcgcccc gtaattccct gtcggtaagt aattaacggc cacttcgcta
     5581 ttgattccag cgtcaatggt gtaatttgcg ttaagggatg atcgagtgga acaagcaaac
     5641 tatggtgcca acaaaaccat gggaaggcga cgagctgcgg gtcattactc aaacgctcgc
     5701 tggcgatacc aatatcagct tcgccatttt gcaacaacgt cgcaatttcc tgtggcgaac
     5761 cctggattag ctcgagccga acctccggga aaagttcgcg aaaagcttta atgacctctg
     5821 gcaagctata acgtgcctga gtatgcgtcg ttgcaatagt gagaacacca gacgtatcgt
     5881 tggtaaacag gtctgcaagc cgacgaacat tactggcttc attcagaata cgttctgcaa
     5941 tgaccagtaa tgctttgccc ggttcagtca tccccagcag tcgcttacct cgtcgaacaa
     6001 atatttcgat gccaaattca tcctccagtt cccgaatatg acggctaacg cctgactgtg
     6061 aggtaaaaag catattcgca acctctgtca ggttgtaatc ctgacgtgca gcctcgcgga
     6121 ttatctttag ttgttggaaa ttcacggtaa actccgggca gttcagatat cccgttattg
     6181 ttaaagtcta atgcccggca taacaaataa taaaaacccg catcttattc catcccgata
     6241 taacgcttag ctcaccaatt gccactgcct tttttccatc actggagaac taatcactga
     6301 cattaacaac tctttcactg cctgtgcctg tggtgacaag tttgctctgg cgggtaaatt
     6361 taaggacaaa gagagactca ttgaaggagt ggtaatgcgt gacatccacc catttactgc
     6421 gccacataac gaacgcgcag ccgattcagg taatactgca acgcccatgc cgctggcaat
     6481 cgctgcggta agcgtggcaa tagactcaat ttcaccaatg acttttgccg tgagccgccg
     6541 tagggaaaaa gcctcatcaa cacgaagtct aacagcgctg taatcactgg ggagaaaaag
     6601 gttcatttgc gcaatagcat taacatcaac gttttgcccc gggcaatctt gagttcctac
     6661 caggaaaaga tcttctttca gcaaagcctg actggatacc ccagccacag gggaatgctc
     6721 ataaatcacc gccatatcga gttggtgatt tatcagtttt tcgttaagca ctgcaccact
     6781 attttcatga agatagataa cgacctccgg gaattcagcg cgaaccgcct gtaataaggg
     6841 catggtgatg gatgacgcag cggttcctgg cgcaaagcca atcgagactt gccccgataa
     6901 tgactgacca acgttatgca ccgccagttg ggcctgctca cactgacgta aaatggcccg
     6961 tgcatgggta tagagaattt ttccggcgtc tgttggcgta acgccccgtt ttgtgcgaat
     7021 caaaagctgt tgatttaact caccttccag tatggcaacc tgttggctga gcgctggttg
     7081 tgcgatatgc aatacttcag cagcctgggt caggctacca atatctacaa tttttacgaa
     7141 gtatttcagg cgtctgaagt tcatgttgcc tccggttttt aagaatcggc ccaagtgccg
     7201 ccattactta caaccagtat gcaagatgcc tgccagttct attttggcgt tgatgcgcaa
     7261 gctaaccaac tgtcaaataa gagattatga tagattcgtc attggccctt tttatcagca
     7321 gtcgcattca cctgccctat aaaaggaggg tatgcaccac gatggttcat tacccaataa
     7381 gattgaaagc tcaccacttt gttgaaattg gcagcaaaca aacaaaaaaa atgcatttca
     7441 ccctttgaca tcaccatgca ctgccattaa tatgcgcccc gttcacacga ttcctctgta
     7501 gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag tccagtcaga
     7561 ggagccaaat tcaaaaaagc ctgctttcga gcaggctttt tactttctaa ttaccaacgc
     7621 tcttaaaaca tctgtcttga accagaacta atttgcacag gcattcccga tcgatgttgc
     7681 aacgcagcat ttgcgcgatt tacatcaact tcttgcccgt tgataaacgc ccgcaaagat
     7741 ggggttaccg gcaatggcac ttttcggtca gactcatatt ctgcacgatt gcgcgacagc
     7801 ggctcatgaa cttccagcca ctttgaacca tccggttccg ttgtgtattt cactggctga
     7861 tcaataattt gtacacgagt gcctacagga acattatcaa acagatattt gatatcgtca
     7921 ttacgcagac gaatacagcc ctgacttaca cggagcccaa taccaaaatt ggcattggta
     7981 ccgtggatgg catacaacct gccaatataa atcgcgtaca gccccatggg gttatcgggc
     8041 cccgcaggaa caaatgcggg caaactctcc cctcgtttcg catattcgcg ccggatgttc
     8101 ggcgttggcg tccaggttgg tgcttcttgt ttacgttcaa cggtagtcac ccagttacgc
     8161 ggggtttctc gcccagcctg gccgatacca ataggaaaga cttccactgt attactgtct
     8221 ggcggatagt aataaagacg catttcagcg acgttaacca caatcccttt acgaacagtg
     8281 gcgggcaaaa tcagttgctg cggaatggtg agttgtgagc caggcttcgg caaaaaaaca
     8341 tcagcgcccg gattcgcttc cagcatatta cttaaccctt gcccgtattg tgcggcaaaa
     8401 gtctccagcg gctgagtatt gtgatcagga acagttacag taagcgactg ccccactaaa
     8461 cggctaccct ctggaggtaa tggataagtt accgccaggc tattttggct ggcaaaaagc
     8521 agagcaaatg agcaaagaat atttacacga cgcatcatgt ccctttccta agtcgcgaat
     8581 actatccgat aagtatagct tttatcagac ttttcgtttt tagctgttca aatcagaagt
     8641 cgtattcccc ggtagaacaa tattactggc agcaagttcg cccatgttgt tgtatatcgc
     8701 acaagcagct tcgatgatgg acatcgccag agctgcacca ctcccctcac ctaaacgcat
     8761 atccatattg agataaggct ccagccccaa atgcgagagc gctatacgcg cgcctttttc
     8821 tgccgacaag tgagaaggaa tgagatacgg tttgattgca ggagacatct ggcaggctgc
     8881 gagcgcagca gcataagaaa gaaatccatc cagcaacacg ggtaaaccgc aggaagcagc
     8941 acctaacatc actccagcta ttccgaccaa atcaaatcca cccacttttg ccaggacatc
     9001 aacaccatcc tgaggatttg gttgattcaa cgtaatcgcc cgacgcacaa catcaatttt
     9061 attagccagt ttatctgtcg gcaggtttgc gccaatccca accacttctt caggatcccg
     9121 gccagtgatt gtgctgacta ttgccgctgc cggtgtcgtg tttgccatcc ccagttcacc
     9181 tacaccaaac agcgtgacac cgtttttcgc cagctcccgc gtataacata tgacgtccaa
     9241 aagcaacttt tcagcctgac ggcgactcat tgccggagct gaagcaatat tgccgctacc
     9301 tcgtgcgaca cgcatgttga taagcccggg gataggctca gcagtatcaa taccaacatc
     9361 aattacgtgg acgttagcgc ccgcttgtgc tgccagcaca cacacgccgg ttgttccacg
     9421 ggtcatattt tcagcctgta tggctgtcac ttcttttgga gaaatagcga ccccttcctc
     9481 ccagacgccg tgatcggcac acataaccag taccgctttt ttgcccacat gcggtatgcc
     9541 attcaccccc ggcattcctg ccagttgtat ggcaagcgcc tccagctttc ctaggctacc
     9601 aacaggtttg aataacccgt caacatgccg ttgtgcacgc gacatagcgg cagaattgat
     9661 ggctgggatc gtattcagta aatcggcaat tatttgcatg tcacgtttct ttatagcagt
     9721 gccagcagga agaccagttc accaagctct atggctgctc ccagcgtatc gcccgtttgc
     9781 ccgcccagcg tacgtttgag aagctgaccg aggatgaaaa tcgccaccat cgtcaccacc
     9841 atagcagcca caccatgcat acccagcaat aatacggcag caaaaatagc ggctaagccg
     9901 agtgtgacgc aggtttgtcg cccatcaatt ttgccaatga acacattgcc aagcccttct
     9961 tcccgcgcat aacgatgacg atacatcaat aatgcggcag tcccgcggct gaccgcacat
    10021 gctgccgcca gcgatgcaag gatcggttcg ccacgcagag ccaactcgct taacaccaga
    10081 atctttgcca gtaccacaaa aatcaatgcc agaccgccgt gggtgcctaa acggctatca
    10141 cgcatgattt ccaacatgcg atcgcgacta cgtgcagaaa atacgccatc acaggtatca
    10201 gcgaggccgt ccagatggaa ttccccggtc atcagcgcca gcacaagtac gctaaacagt
    10261 gccgccagtg gtgcgccaca ccatgcctgc agcaccatga agaccagccc gctaatcgcg
    10321 ccaagcaata atccaatcaa aggaaaagta ataataccgc gagaataatg ttcgaaatcc
    10381 agtccctggg accagcgacg cggtacaggc agacgcgtaa taaatgagag catcgcccaa
    10441 aataatttgc tcatttgatt tttactccaa tacccgaaac caccagccat acttcatttg
    10501 ccgcagcggc caactgctga tttacccgcc cggcaatatc acgaaaatgt cgtgccagac
    10561 gactctccgg cacaatcccc attcccactt cgttagtcac taatacaacc tttgcggggc
    10621 aacgttggca ggcagcaatc aacgactgaa tctcagcatt aatcgcctgt tccatcgcct
    10681 gataatccca ttcatcaggg tctttatcgc cgccataatc aaacaacaga ttagtcacca
    10741 ttgtggtaac gcattccagc aacacagcct cattcgggtt aatgtctgca tgaattaatt
    10801 catcaagatg ttgccagcgc tccactgtgc gccagtgctc cgggcggctt tgccgatgat
    10861 gttctatccg tgcagccatc tcatcatcaa ggatttgcga ggtagcgata tacagaacct
    10921 gtgaagagtg aggtgtactg gcaatagcgg acactaccat tt
//
LOCUS       DAETQL010000118        10646 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.118, whole genome shotgun sequence.
ACCESSION   DAETQL010000118 DAETQL010000000
VERSION     DAETQL010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.118"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(386..820)
                     /locus_tag="K0W65_003545"
     CDS             complement(386..820)
                     /locus_tag="K0W65_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HBI3096658.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(808..1209)
                     /locus_tag="K0W65_003546"
     CDS             complement(808..1209)
                     /locus_tag="K0W65_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096659.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(1469..2038)
                     /locus_tag="K0W65_003547"
     CDS             complement(1469..2038)
                     /locus_tag="K0W65_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HBI3096660.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(2783..2926)
                     /locus_tag="K0W65_003548"
                     /pseudo
     CDS             complement(2783..2926)
                     /locus_tag="K0W65_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(3260..3526)
                     /locus_tag="K0W65_003549"
     CDS             complement(3260..3526)
                     /locus_tag="K0W65_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096661.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            3667..3873
                     /locus_tag="K0W65_003550"
     CDS             3667..3873
                     /locus_tag="K0W65_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBI3096662.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            3968..4570
                     /locus_tag="K0W65_003551"
     CDS             3968..4570
                     /locus_tag="K0W65_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096663.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            5770..6687
                     /locus_tag="K0W65_003552"
     CDS             5770..6687
                     /locus_tag="K0W65_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096664.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
     gene            6772..7644
                     /locus_tag="K0W65_003553"
     CDS             6772..7644
                     /locus_tag="K0W65_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HBI3096665.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            8016..>10646
                     /gene="ag43"
                     /locus_tag="K0W65_003554"
     CDS             8016..>10646
                     /gene="ag43"
                     /locus_tag="K0W65_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HBI3096666.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     HYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFS"
BASE COUNT         2559 a         2774 c         2749 g         2564 t
ORIGIN      
        1 ttcgcctgcc gggagtcaca tttttctgca tatctgacac tttggttccc actacttatt
       61 tggtggacac cactttgtct aattcgtcag attctgacca gacggttcag gctgtacgct
      121 taccactatt ttatccctgt tgaatttggt taagccggtg cagacgcgcc gggaaacgca
      181 cacctgaacc ggcacaataa aatacaatac aaccccctgt tactggagat acgaatggaa
      241 tgacgaggca gcctgtcagt cacagggggc tgttggcata aaatcaacgg aaaggccacc
      301 aacaccggaa ttaagctcca cagtccccgc actggcaatc cattcccggg aaaggcatca
      361 ccgcaaactg ctccggaggc aaccattaca gcctctgtaa cggattatca gccctggcat
      421 cttccgacgg cacctcctgc cgcccccacg ccctgctgta taccccggta ccgcagcgtg
      481 ggcagcactt ttccccctca taatcttcgt cacacatcac acaaaaccac tttctcacct
      541 tcaccgtata atccggaacc gtcatgccct gcagatgcaa acgacgggcc tctttctctg
      601 ccgggtcaag ccaggtacat cgcagcagga ttttttcagc gacagtcatc tggtcatgtt
      661 caaaccacgg tggttgtccg tcattgcccc gctgaagtgt taacgggcat ccacaggaca
      721 cacagcacaa aacgtcctgt cggttgtgct tcgcagcttc tttcgccgta acaaaatggc
      781 ctttgctgtc attcgccata taacagttca gaattcgcat atttcctcct gagttgtccg
      841 gggaatactg aatcctccgg tctggcagtg tgtgcagtac tgctccccat aataatcgtg
      901 gtgacattgt ctgcagtgcc agctggcttt acgcaccacg ggtaaggcat ccggtacgaa
      961 ttgctgcaga cgtttaatca actgtatttc tctgcgctcc ggcctgacat aagggcactg
     1021 ttgaccgtgc tctgtcagcc tgtcgtcagt gtgttcaaac cagggaagtt cagtttcgta
     1081 ttgcggatgg tatctgagtg cactgccaca caagtggcag gtgtagttag cataaggtgc
     1141 ggcctgtgca gtacgggcgc ccgtcagacg tccgttgcca tcaagagcga taaaggattt
     1201 tgcgtacata gtcatatacc tcagccgcca gacgacacgc aggcaacagc cgtcccggta
     1261 cgggcagcgt ggtcagggtg tgaatggcgg ttcagttaaa atctggactg gagtttgggt
     1321 aaagcaggct ggttgctgtt acagcactgt ggttgtcaca caatggaaca ctgacgtaaa
     1381 aaacagtcag tgggcgagtg gatacatcgt tgccgccaga caacaaccca tacttttggt
     1441 ttgccggata ttggcgcgcc agtcgtgctc aactccggct acagccaaaa ttgcgctcac
     1501 cggtgcagtg agctttggta tgctccttcg ccagatagtc agcacgttcc agcacttgct
     1561 gaaagccggt gtcatcatta cgcgccagcc acaccgccgg ccaggccgga aaatgcacca
     1621 gcgtggcatg gcacccgaca tccactccca gggcactgca ccacgcctgt ttaatcatcc
     1681 cggccagaga ctccggagcg cggtaatcac cggcacgaca ccaggtatcc cggttgacca
     1741 gcagcatcag gtgatagtgt tttttacctt tgcactcccc aaactcccgg gcccagacgt
     1801 aatgcagggt tgtgggatgc acgcgtttac cttcccgatg cttacgtttc tggtaagcgt
     1861 cgattcgggc tttcagggca ttgatgaagc gggatatcac agctgcgtcc gttgctgccg
     1921 gtacatccgg gagacgcagg tcaacccgca atgccgtcag gcggggatga acattcagtg
     1981 cgtgccgcac cgtctcacga atacgttgct gccagaaggg gttgtatttg taggtcatgg
     2041 ttaaatctcc gtatggttca tacggaatag ccacgtcgta aaaaatacgc agagtccctg
     2101 aagtgacctc cggcaggaca ctgtctcagg cactctgtga taacccgaac ggtcgtctcc
     2161 ggactgacgg ttgaattttc agcgtcgttt tttaatgtaa aaaaccggtt acgggtaagg
     2221 ctgtgaggag ggagtgatgg tgatacgcaa aaagaagtgc agggactgtg gaaacgcgat
     2281 tactcataat acagtgtgtt gcccgtattg tggttctgtc gaccccttcg gctactaccg
     2341 gaatacagac cgtatcgtta ccatactgct ggcgctgatt attgtggtcc tgctgactac
     2401 tgtcagcgtc agtgtgtgta tactgtgtag ctggtgagat aacggttcat gtatcccctg
     2461 agctgacagt gtaccgtgta tattaagcgt aaccgacagg ttctccttca taccatatta
     2521 aattttccag ggattgccgc tgccttattt cgtcccatac aggaaaactc atctgggttt
     2581 ctgacaaata cgcgaaactc acagcccctc aatatcaacc acacgttgta agccggtaat
     2641 acctgaaaat cagcctatta ccgcccacgc ctccggcacc attccctttt gatttcgaat
     2701 tctttgttgt cgctataccg cttaatgttc ccggtcctcc ggtgcagata acaccggagt
     2761 catcgtagcg ctgcttcaat attcaggtga cgtatttcca gacggaagcc ggcgcgcgat
     2821 cgtaaagttt gttcattgtc agttctgcca gccggttgtc aacggcagag ttaaaggctt
     2881 ccagctccgc aagggtaagt ttataacgcg tgtgggaatt cacttttccc gcgtctcctg
     2941 acgtattacg ggctgccact tacctggtgt cccgcaatac agccttagtg ttccgttatc
     3001 ggggatccgg acgcaccgca acaaccgggg aagtatttct ggtttagagc agtgtcaaga
     3061 gggatttctc agcccctcac tcaccggctc agtctcaggg caggagcatt aatacgctca
     3121 gttcatgtct tatacaggaa tgggcacagg gggagctggc gtgtcactgc tgattttacg
     3181 catcagcgtt catgtatctg cactctgata catgaacgat tcacagcgct ctgttaccgg
     3241 agagatttac tctcttagct tactctggca aatcctttac gttactctct gatgacttct
     3301 tatatttata ataccaatca gacagcctta tcccatcggt gatatcagtc tcctcctcgc
     3361 caggaacggg ctgactcact gtaactacct ccgaaaacaa ctctgcaatt atcagaacgc
     3421 ctgcttctct ccctgtcctc acgaaaacta tcccctcttt atcgcgcgtg cgtgcggaag
     3481 catcttttcg caacaaccac ccggtatacc ggtacggctc agccaccgca acctccccgt
     3541 ttatctcctg gcggtcacat tcccgattat tttttacgtt tctccccggt tgttatgccg
     3601 gtgaaggtgg cgcgctgttt tcatgaccac accggctggt ttaacaacat ccggaggaac
     3661 attctcatgg ctaccccagt ttcactgatg gatgaccaga tggtcgacat ggcatttatc
     3721 actcaactga ccggcctgac agataagtgg ttttacaggc tcatcaggga tggggccttt
     3781 ccggctccca tcaagctggg ccgcagctcc cgctggctga aaagtgaagt ggaagcctgg
     3841 ctacaggcgc gtattgcaca gccccgcccg taatttctga ccgttatccg ttcacccgca
     3901 gcagacgtaa tccccggcct gccggcggca ttctgctgcc tgttatatcc ccgtgaggaa
     3961 tatgaaaatg aaacaacagt atcagacccg ctacgaatgg ctccacgaaa gctaccagaa
     4021 atggctgacc ggcttcaccc ggcacgccgt atcctggggc gtgtgtcatc cgaatatcta
     4081 ctacttccat aatctgacgc ccggttgggt gtcattcaat ggtgaaaaac cggagattgc
     4141 catcgtaccc cagagcctgc accggctgat ttatggccct gacaaacggg ccacgccgcc
     4201 cctggatgat gatctgatag tgaatttatg caccagtgaa catctgctgg ttcatcatcc
     4261 gatgctggaa ggcattctcc tgtctgaatg tgaacgtctg aggcagcgtt ctctggcaaa
     4321 taaactgatc agcctcttcc gtcagtttgg tggcacggag ttgcgcctca aactggtctg
     4381 gctttgctgg cttgatttaa tgaccggaaa cagccttgaa gactggaaag agaacctgaa
     4441 acggaaatca gaaaaagagc tggaggaatg gatcatcaac cgtcagaggc agagcgcagc
     4501 actgacggat ctgatggatc agtacgtgct cctggcatac cgcacaacgg ttgacgatag
     4561 ccgcaactga tgccggttgc ttccggaagg tgtgacaaaa tacggaaatg atgatgctcc
     4621 cggcccccat gctcacctgc agagcgtgtt cgtcgtcatc atactgagcc atgacgggcg
     4681 tcttaagggc atcctgtcat tgctgagcag gcaacaccac cagaggagcc acggacaggg
     4741 atgttcttta cagcccgata cacgccaggt tctcagcaga ctgaatgtgc aggtgcggtt
     4801 attcaccaga aatggcatgc tgcggtacat acccatcctg gtgacaatat gtacgtggcg
     4861 acagcgatcc cggcactgtg gaagagactc actcagcctg caggatattc tttccggcat
     4921 ccgggaatgt ccgcctgaat gcaaacagga tgatatttga atccgtaata ctgttctttc
     4981 atcataaata atttatgcag atacagttaa ccgttacagt agccggtttg ttttaccggg
     5041 aaataatatc cgtcagactg ataccggaac atatctgaaa cacgttatga tgttttgtta
     5101 tcagaaatgt tgtatattct tatccgtggc gacagtttaa tgccctctgg tatcacctga
     5161 taatgtatat cctgcgatga ttcgttatat ttacgggaca cgcctctttt cattgtgctc
     5221 cttttcagac ttcattttcc gtaagtgttc cccggcataa aggaggtacc tttctggcgt
     5281 ttataactct ctttatcggt gacgccggta atacagggaa taccacaccg acaaacgagg
     5341 tattaacaga cgatatttca gtgtccataa tttttctgtc cggttaaata acgtcagtct
     5401 gctgccctct gactgtaatc atcaaagccg gaatgcatac cgggttacaa cgtaaaaaaa
     5461 gtgatgctgg cccccggtgc agctggcggg cgaaacatgt ttacctttca ataccccctg
     5521 tctgcagtct ccactcttcc atcctcttaa gctacgtcgg ccacatgagt gcacttccat
     5581 gataacaggt attcctgtca gcataacgaa gccggttacc ttcccttcct cctgcatttc
     5641 ctgtcagaat gactatacag cgcgcagatt gcctttttat tgaccatctt aatataccgt
     5701 tataaacttc tttttatcat attattatca ccttattatg tttaattttt aagcagtcgg
     5761 agaaagagga tggttcagaa aattttgtcg gataaggtta tgaatgaaag aacaaatgcg
     5821 tattactctt attatcttgg agagcgtaat atctccgtac tgccccttaa tgtttatgat
     5881 cccccggaac gttttatcgc ttacataaaa aaaaacagag aaaatctgaa catcacactt
     5941 tcagattttg aacttgaaca aataatttct ggtatgcgtc tgaaggccct ggccttcctg
     6001 gtccccctgg aaaaaatatc ctggatcgcg ggcagtgaac gcgcatgctt attttcctgg
     6061 tatctgttga tgcagtttat ccagaataac agggcaaaaa taagcgcaga cttgcttcaa
     6121 aagaataaac tgtatcttaa agaagagtat ctggaaggga atgccttccc gtcggactca
     6181 tcgacacagt tcagacaaat actcagagtt ctcgatattc tttctgataa gaatttgcgg
     6241 gatgagtgga taatccagac caaagacaga tggatgcggg catttaaatc aaagagtcct
     6301 ttcagttacc tcctcccgga gaacgaacat gaatgcatat ggacatggaa ttatttgaag
     6361 ggaaaaaata ttgcactgga aaaactggcc agttttcctg gttctgctga tatttaccac
     6421 gcaattcacc tttcatttga catatgggtt acctgtccac tcacctcacc tgacgatata
     6481 aaaaatttca gaaacagttt caataaggcc aaggcgcagc gcaagtacaa aaaaatgcag
     6541 gaagataaag tcaatgtaca gttttttctt gacgcagaaa ccagggcgca gctcaaagaa
     6601 ttatcgaggg tcagaagact cagtaccggc gaaatgctgc atgacctgat tgttgaagag
     6661 tataaaaggt accgacattc cagataattt cagacatata ttatctccgt gagtcccccg
     6721 ccacctttcc ggtgtgcggg ggttttgtct tttttcaccg ggaatacatg tatgaatccg
     6781 tctgatgcca ttgaggcaat tgaaaaaccg ctctcctccc tgccttactc actttcccgt
     6841 cacatcctgg aacatctgcg caaactcacc agtcacgaac ccgtgattgg cattatgggt
     6901 aaaagcgggg ccggtaaatc ctcactctgt aatgcactgt ttcaggggga ggtcaccccg
     6961 gtcagtgatg ttcacgccgg cacccgggaa gtgcagcgtt tccgtctgag tggccatggt
     7021 cacagcatgg ttatcactga cctgcccggg gtgggcgaga gccgggacag agatgcagag
     7081 tatgaagccc tgtaccgtga cattctgcct gaacttgacc tggtactgtg gctgattaaa
     7141 gccgatgacc gtgccctgtc tgtggatgag tatttctggc gacacatcct gcaccgggga
     7201 catcagcagg tgctgtttgt ggtgacgcag gccgacaaaa cggagccctg ccatgaatgg
     7261 gatatggccg gtattcagcc ttctcccgca caggcacaga atattcgcga aaaaacggag
     7321 gcggtattcc gtctgttccg gcccgtacat ccggttgttg ccgtatcggc ccgcaccggc
     7381 tgggaactgg atacgctggt cagtgcgcta atgacagcac ttcccgacca tgccgccagt
     7441 cccctgatga cccgcctgca ggacgagctg cgcacggaat ctgtccgggg tcaggcccgt
     7501 gaacagttta ccggtgcggt ggaccggata tttgacacgg cggagagcgt ctgtgttgcc
     7561 tctgttgccc gtacggttct gcgtgccgtc cgtgacacgg tggtctctgt tgcccgcgct
     7621 gtatggaact ggattttctt ctgaacagtc tgccagggaa atgcctgata acattacgtt
     7681 aaccaactga aaacatgaca caaacccctc atactcctcc tgtttctctt gcgtgaccgc
     7741 cttgtcgtta atagaataaa actatcgata aaacagatat cgatcgttgc tatcgatcgt
     7801 ttatagtggt taatggtctg cgaaaaggca gctgaatctc ttcatcatgc agaacggaat
     7861 tgcacacaac ggactgatac ttctctggcg gatgagagag gggaagcatt tatgtccggg
     7921 aaaaaccact ttatctgacc atgctgtttt gtacctgccg gtatccactt ttgtgggtac
     7981 cggctttttt attcaccctc tgtaaggaaa agctgatgaa acgacatctg aacaccagct
     8041 acaggctggt atggaatcac attacgggca ccctggtggt ggcttccgaa ctggcccgct
     8101 cacggggaaa acgcgccggt gtggcggttg tgctgtctct tgctgctgtc acatcagtcc
     8161 cggcactggc tgctgacacg attgtacagg cgggagaaac cgtgaacggc ggaacactga
     8221 caaatcatga caaccagatt gttctcggta cggccaacgg aatgaccatc agtagcggtc
     8281 tggagtatgg gccggataac gaggccaata ccggcgggca atggatacaa aatggcggta
     8341 tcgccaacaa cactactgtc accggtggtg gtcttcagag agtgaatgcc ggaggaagcg
     8401 tttcagacac ggttatcagt gccggaggcg gacagagcct tcaggggcag gcagtgaata
     8461 ccactctgaa cggcggtgag cagtgggtac atgaaggcgg gattgcaacg ggtaccgtca
     8521 ttaatgagaa gggctggcag accgtcaaat ccggcgcaat ggcaaccgac acggttgtga
     8581 ataccggcgc ggaaggggga ccagatgcag aaaatggtga taccgggcag tttgttcgcg
     8641 gaaatgccgt acgtaccact atcaatgaaa atggtcgtca gattgtggct gctgaaggaa
     8701 cagcaaatac cactgtggtt tatgccggcg gcgaccagac ggtacacggg catgcgctgg
     8761 ataccacact gaatggcggt taccagtatg tgcacaacgg cggtacggcg tctggcactg
     8821 ttgtaaacag tgacggctgg cagattatca aggaaggtgg tctggcggat ttcaccaccg
     8881 ttaaccagaa aggcaaactg caggtgaacg ccggtggtac cgccacacat gtcaccctga
     8941 aacagggtgg agcactggtt accagtactg cggcaacggt tcttggcagc aaccgtctgg
     9001 gcaatttcac ggtggaaaac ggtaaggctg acggagttgt tctggagtcc ggcggtcgcc
     9061 tggatgtact ggaaggccat tcagcgcaga aaacacgggt ggatgacggc ggaaccctgg
     9121 cagtgtctgc cggtggtaag gcaacaggtg tcaccatgac atccggtggt gccctgattg
     9181 cagacagtgg tgccactgtt gaggggacca atgccagcgg taagttcagt attgatggca
     9241 catccggtca ggccagcgga ctgctgctgg aaaatggcgg cagctttacg gttaatgccg
     9301 gaggactggc cagcaacacc actgtcggac atcgtggaac actgacgctg gctgccgggg
     9361 gaagtctgag tggcagaaca cagctcagta aaggtgccag catggtactg aatggcgatg
     9421 tggtcagtac cggcgatatt gttaacgcag gggagattcg ctttgataat cagacgacac
     9481 cggatgccgc gctgagccgt gctgttgcaa aaggcgactc cccggtaacg ttccataaac
     9541 tgaccaccag taacctcacc ggtcagggtg gcaccatcaa tatgcgtgtt cgccttgatg
     9601 gcagcaatac ctctgaccag ctggtgatta atggtggtca ggcaaccggc aaaacctggc
     9661 ttgcgtttac aaatgtcgga aacagtaacc tcggggtggc aacctccgga cagggtatcc
     9721 gggttgtgga tgcacagaat ggcgccacca cagaagaagg tgcgtttgcc ctgagtcgcc
     9781 cgcttcaggc cggcgccttt aactacaccc tgaaccgtga cagcgatgaa gactggtacc
     9841 tgcgcagtga aaatgcttat cgtgctgaag tccccctgta tacatccatg ctgacacagg
     9901 caatggacta tgaccggatt ctggcaggct cccgcagcca tcagaccggt gtaaacggtg
     9961 aaaataacag cgtccgtctc agcattcagg gcggtcatct cggtcacgat aacaacggcg
    10021 gtattgcccg tggagccacg ccggaaagca gcggcagcta tggcttcgtc cgtctggagg
    10081 gtgacctgct gagaacagag gttgccggta tgtctctgac gacaggggtg tatggtgccg
    10141 caggccattc ttccgttgat gttaaggatg atgacggttc ccgcgccggc acggtccggg
    10201 atgatgccgg cagcctgggc ggatacctga atctgacaca cacgtcctcc ggcctgtggg
    10261 ctgacattgt ggcacaggga acccgccaca gcatgaaagc gtcatcggac aataacgact
    10321 tccgtgcccg gggctggggc tggctgggct cactggaaac cggtctgccc ttcagtatca
    10381 ctgacaacct gatgctggag ccacaactgc actatacctg gcagggactt accctggatg
    10441 acggccagga taacgccggt tatgtgaagt tcgggcatgg cagtgcacaa catgtgcgtg
    10501 ccggtttccg tctgggcagc cacaacgata tgagctttgg tgaaggcacc tcatcccgtg
    10561 acactctgcg cgacagtaca aaacaccgtg tgagtgaact gccggtgaac tggtgggtac
    10621 agccttctgt tatccgcact ttcagc
//
LOCUS       DAETQL010000119        10467 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.119, whole genome shotgun sequence.
ACCESSION   DAETQL010000119 DAETQL010000000
VERSION     DAETQL010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10467
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.119"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            5..205
                     /gene="cspG"
                     /locus_tag="K0W65_003555"
     CDS             5..205
                     /gene="cspG"
                     /locus_tag="K0W65_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HBI3096667.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            216..404
                     /locus_tag="K0W65_003556"
     CDS             216..404
                     /locus_tag="K0W65_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBI3096668.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            379..609
                     /gene="ymcE"
                     /locus_tag="K0W65_003557"
     CDS             379..609
                     /gene="ymcE"
                     /locus_tag="K0W65_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HBI3096669.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            599..772
                     /locus_tag="K0W65_003558"
     CDS             599..772
                     /locus_tag="K0W65_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HBI3096670.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(821..1894)
                     /locus_tag="K0W65_003559"
     CDS             complement(821..1894)
                     /locus_tag="K0W65_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBI3096671.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1996..4707)
                     /gene="torS"
                     /locus_tag="K0W65_003560"
                     /pseudo
     CDS             complement(1996..4707)
                     /gene="torS"
                     /locus_tag="K0W65_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4790..5818
                     /gene="torT"
                     /locus_tag="K0W65_003561"
     CDS             4790..5818
                     /gene="torT"
                     /locus_tag="K0W65_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HBI3096672.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5791..6483)
                     /gene="torR"
                     /locus_tag="K0W65_003562"
     CDS             complement(5791..6483)
                     /gene="torR"
                     /locus_tag="K0W65_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HBI3096673.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6570..7742
                     /gene="torC"
                     /locus_tag="K0W65_003563"
     CDS             6570..7742
                     /gene="torC"
                     /locus_tag="K0W65_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HBI3096674.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDITGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7742..10282
                     /gene="torA"
                     /locus_tag="K0W65_003564"
                     /pseudo
     CDS             7742..10282
                     /gene="torA"
                     /locus_tag="K0W65_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10279..>10467
                     /gene="torD"
                     /locus_tag="K0W65_003565"
     CDS             10279..>10467
                     /gene="torD"
                     /locus_tag="K0W65_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HBI3096675.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2526 a         2684 c         2789 g         2468 t
ORIGIN      
        1 taaaatgact ggtttagtaa aatggtttaa cgcagataaa ggttttggct ttatcactcc
       61 tgatgatggc agcaaagacg ttttcgtcca tttcaccgcc atccagagca atgaattccg
      121 cacgctgaac gaaaatcaga aagttgaatt ttctattgag caggggcaac gtggccccgc
      181 ggcagcgaac gttgttacgc tctaaggttg ccgttattac tcaacatctc catttccgct
      241 gtccatgttg tcatggttca cagtaccgta catcggcatt cgatgtgacg gagcgaaacc
      301 ctttgggcgc taagtgtatt ttttgtaaat cgactatgat cacctttgat aacgtcgcgc
      361 tgcaaatacg cactgaccat gcgccgctgg atttcacaaa ataatatcag gctccctcgt
      421 ggagcctttt ttatatctgc cttatttttc ttcaacgctg tatgtatagt aagcgataac
      481 ctgttgatta ttgaatcttt cgggaagatg gcttataaca tttcttacct gaccagggta
      541 ccgggaacca acaccttact ggcgtgttgc tgtctttcaa gaccagacga ggttaacagt
      601 gaatattgaa gagttaaaaa aacaagccga aacggaaatc gccgacttta tcgcgcaaaa
      661 aatcgccgag ctgaacaaga atacagggaa agaagtctct gaaattcgct tcatcgcacg
      721 agaaaaaatg accgggcttg aaagttatga tgtcaaaatc aaaataatgt gattttgtga
      781 acatcacccc gtgcgaggtg atgttccgct tgttgctaat ttagtgacca atcattggcg
      841 cttgtggaat taagcgtcgg tacaattcct ccggcaccgg gctttgccat actcccgcat
      901 acattgcgta accaatcacc gcaaacataa tccccagaac cagtagcgtc attaaccagc
      961 cagacaacgc aaaggctttt ttatttgctg caggtttttg cagtgaaaag gtcaatgttg
     1021 aggctaccgg acatgactct acgcaagtca tacagccggt acattccact gttcgtacct
     1081 gaattaattt atctaccggg atccgtgatg ggcacttttt tgcgcatttg ccacaatcga
     1141 tacaactttc ggcattgcga cgaatcttaa acggcgacaa tagcgaaacc acgcccatca
     1201 gcgcgccata tgggcaaaga taacgacacc aggcatggcg aataaacagg ctggcaatca
     1261 gcaaaacggt cacgctgatt aatgtcgcgg tccccatatg acgaaagaaa tcgagcattt
     1321 taacgtccat caccacgcta tagggcgaca acataaaata gtgaatcgcc tgagcgggca
     1381 tcaataacgc gatatagaga aataaactca acagcaaata cttcacgccg cgcagaggaa
     1441 tatccagcca gcggggaagg acacattgcc gaccaaacag tttgttaccg agatcgccga
     1501 ttaattcaga aagcgtacca accgggcata accatgagca aaaggccttt ttgagtaata
     1561 gactgatgac gataaaagcg accaataaca gcatcgcggc ggcgtggacg gacggtaact
     1621 gacctgttgc aaggctatat ttcagattca tcagaccggc aatcggtagc cagccttcga
     1681 tacctcccgg tctggcgaca aatgtcgtac tacctgccgt ttcgtaatag cgcacccaat
     1741 accagaacgt gatggcaata taaatattca ttgccaatag taagaattgc gtcgctttac
     1801 gccaggtcgt ggcattgcgc cagtcattcc acggtaattt gccgcccgtc gtgcctggcc
     1861 gccgctgcca gcgggttctt tttttctctg ccatgatttt gccagtccgt taagttgcat
     1921 accaaatgcc actattctag ttattcttaa ctggctgata ttgattcaaa tcgcgttcag
     1981 gtctttctta ttcaatcatg cttccagagc ggcaacactg cgtgtaattt cttcgtgtgg
     2041 aaggggggca gataatggct gctgctccag ttgtgcgcat agctgactgg cgctacgcat
     2101 tcccagactc gagcaactgc tttttagctg atgcgcggca cgctttattt tctcgctgtc
     2161 ctggctggcg cgggcaatgt cgatttcatc gagaagcggc agggcatgtt gtttaaataa
     2221 tgccagccat tcgtggatct tctccgtccc cattaactga gcgtcttcat tgagttgcga
     2281 gacatccagt ggtagatcgt tattggcttg cagttggaga tagtgcgcca gtaactgacc
     2341 gagcacttca cgcggcaccg gtttagggat aatcccgcga aatagtgaac tggtacgctg
     2401 gcgcagcgtt tcgtcaatga catgggcgct aaagccaatc aaaaccagcg acggatattg
     2461 ctgtgccagt tgccgggcaa gggtaatgcc gtcgatatcc ggcagatcaa aatccaccag
     2521 tgcggcagca aacggttcgc tattttgcag tgtctctaaa gcctgcgcgg cattgccaat
     2581 agcaacaacc tgcgcaccac tggtgttcag catctcaacg gtaattcgct gggttagcgg
     2641 gttatcttca attaacagca aacgtaaacc gtcaagacgc accgcctgat tggctgtttt
     2701 tggcacgggt gcagtggcaa cacgtaacgg caagcgtaaa caaaaacagc ttccaacctc
     2761 cggcgtgctg gtggcgctca gttcgccgcc catcgcctgg gccagacggc tactgatagt
     2821 cagccccagc ccggtgccgc cgcgtttgcc gcttacctgc acaaatggct ggaagatttc
     2881 tgccagtttc gcgggatcaa taccgcagcc gctgtcttcc acttcgacca gccattgctc
     2941 gccatcagtg cgactacgca ggataatgtg cccttcgtca gtaaaacgca gggcattgct
     3001 caataggttg gtaatgacct gacgaatacg tcgtggatcg cccattaacg cgctcggtac
     3061 atcatcggca attgccgttg ccaggcgaat cgggcgacct ttcacccgtc cgctcattaa
     3121 ttgcagggta ctttccagca gcgggcgcgg ttcaaagggc tcatcgctga ctgaaacatt
     3181 tttgccacct gcttcgatag ccgaataatc gagaatatcg ttgaggatgg tcagcaacga
     3241 ttcgccagag tcggtaatcg cctgcaaatc atcacgctgg gcgttaagtg cggggttatc
     3301 tgccagcagt tgagcagtgc cgagaatacc gtacagcggt gtgcggatct catggctcat
     3361 cgccgtcaga aacgccgatt ttgcctggct ggctttttct gcttccgccc gtgcctgtcg
     3421 gtgttctatc accagttcct gcaattcagc tgtacgcgct ttgacctgcg ccgccagctg
     3481 ttcgcggtgg cgattcagtg catgaacact gctgcgaaac gcatccatca gccgcccgat
     3541 ggtatccagc tcccgtacgc cagcggtttc cgggaaaggg gagtcaatat caccgtccag
     3601 cagccgttgc agcgcctgcg tttgttcggc aagtggacgc gtgaatgagc gataaaccac
     3661 gcgccagagg atcataatca gtgcgcaaag tgaaaccatc cccagcaata acaggctgta
     3721 ttgcccgcgt gcactggctt tttccagatg tgccagtcca tgctgattgt gcagctcaat
     3781 ggtgtcgacc agctgactga cttcgctact aaactgcgcg aactgggcga tattattttg
     3841 ggcgagagtt tgcaggtgat tgctgatttc actgtcctgc tggaacagcg ccaacagatc
     3901 gctatattgg ctaacggtag ttaacgttgt tgcgacctgc gcacgaactc ccggatcttc
     3961 aatgcgtatt tgccgacgtt gcagaatttt caccgcatta ttgagctgct tttccagcgt
     4021 tggtgcattt ttctggatct gctccagccc cagattcatc accatttgct gcacccgcag
     4081 agcgctaagg cgcagttcat tcatctggtt aacatactca agatcgatat caatcagccg
     4141 atcgagtgca ctttcagcag cctgacgctg atcttgttcg atcaaatcgt aaatcccggc
     4201 ctgggtcgct ccagcggaag ttgtcgcatt attcgcctga ccttgctcca ggcgtgcgat
     4261 ctcatcggcg gcagctatct gctgactgag ttgccgttgt tgctggcgta gttgcagacg
     4321 ccgccccacc agttcccctt gctgacgtaa tgaacgggag atctcctgct cctgttgttc
     4381 aatagcggtg gtatcaaaac cttgttcccg taacgcttgc agcaacgcat taatcttcag
     4441 gctttatgcg gtgagcattc gcccctgcgc ctgccacatc ttttcgttat cggcactggt
     4501 caggttctgc gcggcgaaaa gttcccaggc gctggcttcg ctcaactggc gcgccatatt
     4561 catggtagga atcaatgcct gagtattgtc tttttccacc tggctgataa agcgcaggtt
     4621 gtaccatccc accagggtac tggtcagggt taacagcgcc atcagggcaa agcccatcca
     4681 gagtcttcgg gtcagggtta aattcacggt cggtgcactt aaggtgaaaa aggttgagtc
     4741 gcaaagtgga atgcatctag cataaagcct tattattgat gaggctatca tgcgcgtact
     4801 gctattttta cttctttccc ttttcatgtt gtcggcattt tcggctgata acctgttgcg
     4861 ctggcatgat gcgcagcatt tcacggtgca agcctctatg ccgcttaaag ccaaacgcgt
     4921 atggaaactg tgcgcgcttt atcccagcct gaaagattca tactggttat cgttgaacta
     4981 tggtatgcag gaggctgctc gccgctacgg tgtggattta aaagtgctgg aggcaggcgg
     5041 ctacagccag ttggctaccc agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga
     5101 ggccattttg ctcggtagta gcacgacgtc atttcccgac ctgcaaaagc aggtagcaaa
     5161 tctgccggtg atcgaactgg taaatgctct tgatgatccc caggtgaaaa gccgcgttgg
     5221 tgtaccctgg tttcagatgg gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg
     5281 taaaccactg aacgtgctgc tgatgcccgg acccgataac gccgggggca gtaaggagat
     5341 ggtcgagggt tttcgcgcag ccattgccgg aagcccggtg cgtattgttg atattgcgct
     5401 tggtgataac gatattgaaa tccagcgcaa cctgttgcag gagatgctgg aacgccaccc
     5461 agaaatcgac gtcgttgccg gaacggccat tgcggcagag gcggcaatgg gtgaagggcg
     5521 taacctgaaa acgccgctta ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg
     5581 gctgaagcgg ggaagagtga ttatggctgc cagcgatcaa atggtctggc agggggaact
     5641 ggcagttgag caggccatca ggcaattaca ggggcaatcg gtgtctgata atgtcagccc
     5701 accgatttta gttctgacgc cgaaaaatgc cgaccgagaa catatccgcc gctcgctgtc
     5761 accgggggga tttcgtccgg tctattttta tcagcacaca tcagcggcta agaaataacc
     5821 ttcaccatgt tgcgtcacca gtaaatccgc gctgagttta tgacgtaaac gacgaattaa
     5881 cacatcgacg gtgcgcaggt cagggttttc cacccgacgc gcagaaagca tacgcagcag
     5941 acgctcacgg ctgagaattt cgcccggatt agtcacaaat gccaccaaca tttcatactc
     6001 tgcgcgggtc agtttaatcg gctcgccatc ccgctccagc gtatggcgcg acacattcag
     6061 gcaataaccc gcaaagcgat agcagtagtc ctgagtgtgc ggttgagctt gtcgcgcgag
     6121 gtcgattcgc cagagcagat ttttcacccg tactaccagt tcgcgcagtt ccagcggttt
     6181 ggtgacgtaa tcgtctgcgc ccatttccag cccaacaata cggtcaatcc gatcgctgcg
     6241 tccggtaacc agaataatcc ccaccgttga gcgttctcgc agggcgcggg ttaacatcag
     6301 gccattttca tcgggtaagt tgatatccag cagaattaaa tctaccggct gattctgcat
     6361 aatttcccgt agcccggcac cgctcgccgt aacggaaacg gtatacccct cctgagtgaa
     6421 gtaggattgt aatcgcgcct gggtaaccgg cttatcttca acaataacaa tgtgatgtgg
     6481 catcagaggg ttttactcat tctgttcata tctgttcata tttgctcatt aagatcgctt
     6541 cactaaacca taattctaca ggggttatta tgcggaaact ctggaacgcg ctacgccgac
     6601 ccagtgctcg ttggtcggta ctggcgctgg tcgcaattgg gattgtgatt ggcattgcgc
     6661 tgattgtatt gccacacgtt gggatcaaag tcaccagcac aaccgaattt tgtgtcagtt
     6721 gccacagtat gcaaccggtg tatgaagaat ataaacagtc ggtgcatttc cagaacgcct
     6781 ccggcgtgcg agctgaatgc catgactgtc atatcccgcc ggatattaca ggcatggtga
     6841 agcgcaaact ggaagcgagc aacgacattt accagacctt tattgcccac tccattgata
     6901 cacctgaaaa attcgaagcc aaacgcgcgg aacttgccga gcgtgaatgg gcgcgaatga
     6961 aagaaaacaa ctcggcaacc tgtcgctcct gccataacta cgatgcgatg gatcatgcca
     7021 ggcagcatcc tgaagcggca cgtcagatga aggtggcagc gaaagataat caatcctgca
     7081 tcgactgtca taaaggtatt gcccaccagt taccggatat gagtagcggc ttccgtaagc
     7141 agttcgatga gctgcgcgcc agtgctaatg acagtggtga cacgctgtac tctattgata
     7201 ttaagccaat ttatgcggcg aaaggcgata aagaagcctc tggttctctg ctgcctgctt
     7261 cggaagtgaa agtccttaaa cgtgacggcg actggctgca aattgaaatt accggctgga
     7321 cggaaagcgc cggacgtcag cgtgtactca cccaattccc aggtaaacgc atctttgttg
     7381 cctcgattcg tggtgatgtg cagcagcagg tgaaaacgct ggagaaaacc accgttgccg
     7441 acaccaatac cgagtggagc aagttgcagg ccaccgcgtg gatgaagaaa ggcgacatgg
     7501 tgaacgatat caaaccgatc tgggcctatg cggattcgtt gtacaacggc acctgtaacg
     7561 agtgccacgg cgcaccggaa atcgcccact ttgacgctaa cggttggatc ggcacgctca
     7621 acggcatgat tggctttacc agtctcgata aacgtgaaga acgcactttg ttgaaatatc
     7681 tgcaaatgaa tgcgtctgac accgcaggta aggctcacgg cgataagaag gaagaaaaat
     7741 aatgaacaat aacgatctct ttcaggcatc acgtcggcgt tttctggcac aactcggcgg
     7801 cttaaccgtc gccgggatgc tggggccgtc attgttaacg ccgcgccgtg caactgcggc
     7861 gcaagcggcg actgaggctg tcatctcgaa agagggcatt cttaccgggt cgcactgggg
     7921 ggctatccgc gcgatggtga aggatggtcg ctttgtggcg gcaaaaccgt tcgaactgga
     7981 taaatatccg tcgaaaatga tcgccggatt gccggatcac gtacacaacg cggcacgtat
     8041 tcgttatccg atggtacgcg tggactggct gcgtaagcgc catctgagcg acacctccca
     8101 gcgcggtgat aaccgttttg tgcgcgtgag ctgggatgaa gccctcgaca tgttctatga
     8161 agaactggaa cgcgtacaga aaactcacgg gccgagtgcc ttgctgaccg ccagtggttg
     8221 gcaatcgacg gggatgttcc ataacgcttc ggggatgctg gcgaaagcta ttgccttgca
     8281 tggtaatagc gttggtacgg gcggagatta ctctaccggt gctgcgcagg tgatcctgcc
     8341 gcgcgtagtc ggttcaatgg aagtgtatga acagcaaacc tcctggccgc tggtattgca
     8401 gaacagcaaa accattgtgc tgtggggctc cgatttgctg aaaaaccagc aagcgaactg
     8461 gtggtgcccg gatcacgatg tttatgaata ttacgagcag ctaaaagcga aagtcgccgc
     8521 cggtgaagtt gaggtcatca gcatcgatcc ggttgtcaca tccacccatg agtatctggg
     8581 gcgcgagcat gtgaagcaca ttgcggttaa cccgcaaact gacgtgccgc tgcaactggc
     8641 gctggcacat acgctgtaca gtgaaaacct gtacgacaaa aacttcctcg ctaactactg
     8701 tgtgggtttt gagcagttcc tgccgtatct gctgggtgag aaagacggtc agccgaaaga
     8761 tgccgcatgg gctgaaaaac tgaccggcat tgatgccgaa accattcgtg ggctggcgcg
     8821 gcagatggcg gcgaacagaa cgcaaattat tgctggctgg tgcgtgcagc gtatgcagca
     8881 cggtgaacag tgggcgtgga tgattgtggt tctggcggcg atgctggggc aaattggcct
     8941 gccaggtggt ggttttggct ggcactacaa cggcgcaggc acgccggggc gtaaaggcgt
     9001 tattctgagt ggtttctccg gctctacgtc gattccgcct gttcacgaca acagtgacta
     9061 caaaggctac agcagcacta ttccgattgc ccgttttatc gatgcgatcc tcgaaccggg
     9121 gaaagtgatc aactggaacg gtaaatcggt aaaactgccg ccgctgaaaa tgtgtatttt
     9181 tgccggaact aacccattcc atcgccatca gcagatcaac cgcattattg aaggctggcg
     9241 caagctggaa acggttatcg ccatagataa ccagtggacc tcaacctgcc gctttgccga
     9301 tatcgtgctg cctgcgacca cgcagtttga gcgtaacgat ctcgaccagt acggcaacca
     9361 ctccaaccgt ggcattatcg ccatgaaaca ggtggtgccg ccgtagttcg aggcgcgcaa
     9421 cgactttgat attttccgcg agctgtgccg tcgctttaat cgtgaagaag cctttaccga
     9481 agggctggac gaaatgggct ggctgaaacg catctggcag gaaggtgtac aacaaggcaa
     9541 aggacgcggc gttcatctgc cagcgtttga tgacttctgg aataacaaag agtacgtcga
     9601 gtttgaccat ccgcagatgt ttgttcgcca ccaggcattc cgcgaagatc cggatctcga
     9661 accgctgggc acgccgagtg gcctgattga gatctactcg aaaaccatcg ccgatatgaa
     9721 ctacgacgat tgtcaggggc atccgatgtg gtttgagaaa atcgaacgct cccacggtgg
     9781 gcccggctcg cagaagtatc cgttgcatct gcaatctgtg catccggatt tccgacttca
     9841 ctcgcagcta tgtgagtcgg aaacgctgcg tcagcaatat acggtagcgg gtaaagagcc
     9901 agtgttcatt aacccgcagg atgccagcgc gcgcggtatt cgtaacggtg atgtggtacg
     9961 cgtctttaac gctcgcggtc aggtgttggc aggagcagta gtttctgacc gctatgcacc
    10021 cggcgtggcg cgaattcacg aaggggcatg gtacgatcca gataaaggcg gcgagcctgg
    10081 tgcgctgtgc aaatacggta accccaacgt gctgaccatc gacatcggga cttcgcagct
    10141 ggcgcaggcg accagtgcgc acactacgct ggtggaaatt gagaagtaca atggaacagt
    10201 ggagcaggtg acggcgttta acggccccgt ggagatggtg gcgcagtgcg aatatgttcc
    10261 cgcgtcgcag gtgaaatcat gaccacgctg acagcacaac agattgcctg tgtttacgcc
    10321 tggctggcgc agttgttctc ccgtgaactg gacgatgaac aactgacgca aatcgccagt
    10381 gcgcagatgg ctgaatggtt ttcgttgctg aaaagcgaac cgccgctcac tgcggcggtg
    10441 aatgatctgg aaaacagtat tgccacg
//
LOCUS       DAETQL010000120        10446 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.120, whole genome shotgun sequence.
ACCESSION   DAETQL010000120 DAETQL010000000
VERSION     DAETQL010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.120"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            597..794
                     /locus_tag="K0W65_003566"
     CDS             597..794
                     /locus_tag="K0W65_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096676.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="K0W65_003567"
     CDS             complement(744..1937)
                     /locus_tag="K0W65_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3096677.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="K0W65_003568"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="K0W65_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HBI3096678.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="K0W65_003569"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="K0W65_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HBI3096679.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="K0W65_003570"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="K0W65_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HBI3096680.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="K0W65_003571"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="K0W65_003571"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HBI3096681.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="K0W65_003572"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="K0W65_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HBI3096682.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2560 a         2655 c         2749 g         2482 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcata
//
LOCUS       DAETQL010000121        10435 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.121, whole genome shotgun sequence.
ACCESSION   DAETQL010000121 DAETQL010000000
VERSION     DAETQL010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.121"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..272)
                     /locus_tag="K0W65_003573"
     CDS             complement(<1..272)
                     /locus_tag="K0W65_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HBI3096683.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="K0W65_003574"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="K0W65_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1132..1338)
                     /locus_tag="K0W65_003575"
     CDS             complement(1132..1338)
                     /locus_tag="K0W65_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HBI3096684.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1445..2008)
                     /locus_tag="K0W65_003576"
     CDS             complement(1445..2008)
                     /locus_tag="K0W65_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HBI3096685.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3383..5218)
                     /locus_tag="K0W65_003577"
     CDS             complement(3383..5218)
                     /locus_tag="K0W65_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096686.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5319..5606
                     /locus_tag="K0W65_003578"
     CDS             5319..5606
                     /locus_tag="K0W65_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HBI3096687.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5578..7107
                     /locus_tag="K0W65_003579"
     CDS             5578..7107
                     /locus_tag="K0W65_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HBI3096688.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7277..8494)
                     /locus_tag="K0W65_003580"
     CDS             complement(7277..8494)
                     /locus_tag="K0W65_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI3096689.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8861..9847)
                     /locus_tag="K0W65_003581"
     CDS             complement(8861..9847)
                     /locus_tag="K0W65_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096690.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9844..10372)
                     /locus_tag="K0W65_003582"
                     /pseudo
     CDS             complement(9844..10372)
                     /locus_tag="K0W65_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3249 a         2386 c         1965 g         2835 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg gggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagagaga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattagaca tagatcacaa aaacacacca ttctaattga
     3241 aaaacaaaac aaacaaataa atcatactat tatagaattt tctcttgcca gacatagata
     3301 atacccctgc taattttgct aatggttagc aaaattagca gggtatataa agattattga
     3361 cccaaaacac actcagatga attcacgaca gctcaggagg gttattcctg atgtcatctt
     3421 cacaaataat ggttaacgca tcatatatca ttctgcgtct gttcctgttt tcggccttat
     3481 cgccatacaa catcatcaca tttttaattt catcgaccgg tgatttcaca tagtgaacta
     3541 atacaccaag cttctcaaca cattgcagta aagtatattt cttactgcct gtgaaaagcc
     3601 ctatcatagt ttctttacgt tcgcgactgg ataatacttt gttttccata atgacttctg
     3661 ccagcgtctt accatcataa acatactctt tatctatcag aatatttatc ttatccagca
     3721 ccttctctac gtcatcaagc aaacctgtaa tatatggggg cgcaatatat ggtttcgtac
     3781 tgatcttttc aagcatacaa ttatacatct taataacacg catcagggta aaattgttcg
     3841 catcatactg tatttcaccc tccttatata caggatcaat aagtggtatt ttttcaataa
     3901 atgcatcctg ataggctttt acatggtcaa gccaataaaa caacgagcca tactttatat
     3961 tttttctatt tactttaacc tccagtgctg tccgaagcaa cgataatgcc cccctgacat
     4021 atcctgaagg taattcacca aacaatcctt ttctttccat gtcatcataa tgctgcagca
     4081 catcattaat tttatttttc cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc
     4141 tggtcacatg aacagcctca ttctgcaact gcccaaaata gcttataatc tttttcagat
     4201 atgcgtcatc acaaccattc ttatcattaa tattaaatac aagatctttc agacgggaaa
     4261 tatattcctg agatttatca tcaagtgaaa cactataacg agacaatgtt ccttctgcac
     4321 tgttttgaaa cttataaaac atttccagta atttttttgc ataaggcgtt gtatcaaagc
     4381 tggtaaagtc ataaagatat tgcgcagcgt aaccatacaa accatgaatc cagagtcctt
     4441 ctctcaccaa cactctgaga taatatgcaa ggctttgaag tatcttcgtc tttcgttgta
     4501 tatcaagtaa gacacttaat gaataaaatg cattatagaa tttatcaata ttcctgagca
     4561 ctttctccag ttcatcctta tccagcttct tctcccggta atccttttta tattcatttt
     4621 tgatagccat gatatattgt tgcatcaggc tcttgctttt cccgttatcc ttccgtatag
     4681 ttcctgtata tttccccaga gcatacagct ttccattttc atgaaaatct tccttgttga
     4741 atcccagttc agccagatat tcccgaagat aacgtttaac agcagcttca tcgctttctg
     4801 tatcagcgtg atcaagacaa ggacaatacg atttctgtaa aaaattataa atatgataat
     4861 aaaaagcaga gtcattctct ggtctgtatg tattatacat atctacaaca agaaaaataa
     4921 ccccaaaagt gcgcagacaa tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg
     4981 gataagaatt catcatgtaa taaaaatgtt catatcgggc atatatgatc cgccgttgct
     5041 cctgactgaa caccccttca gcatcaataa tacgactgat ctcattataa cgttgatccc
     5101 agaatttttg atgcatttca aaattggtac tcttccgctt gtgtgccgga ttctcgcctt
     5161 ccttaaacaa ccccaaaaca gtacaaatat catacaacat atttgtaaat gtgcgcatat
     5221 gctgtgctct ccggttggtt tgcattttat cgcagaggac attctgagct taacgtatca
     5281 aactgacaca acgacatgca atgcatatgg agaacaatat gtcaaatcaa accggacagc
     5341 tttacacgcc ttcacgcatc ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa
     5401 gatctactat ttacgtgaaa ataaatccgg cctcgaaata ccacgaccct acctttccca
     5461 aaccaataag gcttggtagt gcatccgtag gctggcgggc atctgccatc gatgagtggc
     5521 tgatgttaca tacggcacca gcctggtctg aacctgaaaa caaactaaag gaacttaatg
     5581 atgacgacaa aactatcact ccctgaactt gacacaacta aactgcctga caggtgccag
     5641 gcattacttg atgaaatgca tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt
     5701 atgctgacgg tcatggctgc ctcggtccag gatacgcatg aagttgaact ttccggaggg
     5761 caacgtacca gccttcagat atttatgtgt ctttcatcag catcaggcag tggaaaaacc
     5821 tctgcctgcg caaaattaat cgctcctgtt catgaaacag aagaagaact acatcaggca
     5881 tatatcgacg ataaaaaaaa ctatgaccga atgatggaaa tgtggacaac agataaaaaa
     5941 atcctggagc ggagatataa aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca
     6001 cgagcagcac ttgaaaagtg tatagcaaat aaacccgaac ctcccgtaca acaggttctc
     6061 atcgtgaatg acgcgacacc agaagggata gctttgaaac tcagccagtc accctctctt
     6121 ctgttgctgt ctgatgaagg aggtactatt ctggataagc gtttcgaacg caaatcggca
     6181 ctgtacaaca cattatggag tgggcaacca gtcaacgttg aacgggcatc cagacccggg
     6241 ttccggctga atgatgtccg tctcactata cttatactga ctcaaccggt aatatttaat
     6301 aagtttttta ctctcactgg cgatcagatc cggggcaatg gctttctggc ccggttgcta
     6361 ttttgcgaac ccggagcaat taaaataatg acgacagaac cgcatactgc cactcctgtt
     6421 gtgacgcaac aatgtgcaag ctgttttcgt aagaaaagtt ttggcagtca gatcagagac
     6481 tctctgagag catccagaga aagacgtgca aaaagtaaag agcgcatctg catgacatta
     6541 tcaaatgcag catcccgcgc actggagcgc tttcacaaag aaaatatgga tactgtcaga
     6601 cagaatcctc atatgggtac atttgaagac attattgtca gaaaaagaga gcaggttgtc
     6661 aggatagcag cacttctgga actggagaaa gatccagact cgacagtaat aacgctggaa
     6721 agtactaata gcgccattta tctcattgaa ttttatttca agcatcttat atataaactt
     6781 gaatcccttc gggaaatatc cccggctgag aaacttgata aatggcttca gaaaagaatt
     6841 atcacaacag caggttacat attccagaaa agttatattt tacaatatgc cccatatgct
     6901 ctcaggaaaa aatgtgttct ggatgaagca cttgacatac tggcggaaca aaggaaaata
     6961 aggattgatg ataacctggt tgtttatata ggaaacacga ttacaccatc cgaactggca
     7021 aaaaaactaa atattcctgc attcgatgcc ggagttttca tttgtgatca tcaaaacata
     7081 ctgaagtatc accgaaacag actatagata ctatcggaca aaaaataaat tatcacaagt
     7141 gtattattcg ttataataat gaataatcgt tatctgagtg caatgccagg ttttcattac
     7201 gaactggcat tgcatttatt tttcaaaaaa gaaagcagta accatttggc tactctgagt
     7261 atgccagtcc atgaaactaa gcgaattcct tagtgttttt agcaagttga gacatcacat
     7321 aatccgccca tgcctgcatc atcggtacac gctgctccag tagatcagta cgatgatatg
     7381 ccgcctcaac cttatttttc agcgtatgag cgagcgccct ttccgccaga tcccgcgaat
     7441 acccctgttc actacaccag tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc
     7501 gccccggaat gtcactgacg gctttctttt tacgcagaaa acttgtcaac accatatcgg
     7561 aaaggatctg ctgctttctg ggtgaaggga acaccagttc atcatgcagg ccacgtatat
     7621 tttccagaat gtaaatagcc tgccgggata aaggaacacg atgctgtagc ctagctttca
     7681 ttctttctgc aggtatagtc catacccgct tatgaaaatc aatttcagcc cagcgcattc
     7741 ccctggcttc gcccgagcga gttgctgtaa gtatcaccat taataacagt gcgcgggtaa
     7801 cattataagg ttcatcacta tacacactgg tcgccacaaa aagcggtaac tgcctccagg
     7861 gcattgcggg ttggtgttca tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca
     7921 catcaacagg atttgccaca caaaaaccgt gcgcccatcc ccactgcata acaacatgaa
     7981 tgcgctgttt aacccggctt gccgtttctg acaaggttaa ccagactgga cgcagtgttt
     8041 ctgccacgtc cgcagccgta atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt
     8101 aattctcaag cgtcgataac cactgcctta catgctttgg attctcccat ccaggagaca
     8161 gttctgcatg tacacgcctg gctgcatcgg caaatgttgg gataacgact ttctcagatt
     8221 cagccttttt aatctccaga ggatcatcac ctgcagcaag ttgctctcgc attatccggg
     8281 cagtacgtgc agcttcagca atactgacct ctgggtaagt tcccaatcca gcattacgtc
     8341 ttttttgtgt caccggactt acataacgaa aaacccattt cccccgcccc tttactgaag
     8401 aaggatgaag ggtcagtccg gtaattcccc catggggcaa tggtttgtca tcaggtttga
     8461 tatgtcttgc tttcgtatcc gtcaatactg ccatacgcta attcccgtca ctctggtatg
     8521 ccatccagta tgccattagt ccctcgcttc actaagaata cattagataa catcggacaa
     8581 caaaaagtgt aatacattgt ttataaagaa attatataac acccctggat taagtcagat
     8641 ttatttcagg cggtccccct caccgccata tttaaagaag agcgcgtacg aaagtacggg
     8701 cttttttttc gtatattgta cacaccgggt gcccccgccc acgtctgtta gggcgagggg
     8761 aaattgtgca catcaactgt ctggttactc agtcaacaat caaaatgcgt aagatagtac
     8821 agaacatata tgcgttatct ggtcacttcc tgtaacttcc tcacttatta aaatgccccc
     8881 aacggaaata catgtcacat gccatcataa aatcgttatg ccagaggaat cgacaatgga
     8941 aaaaatagta aattaagcac ggtacatagc agagtaaaaa ataggcttca aaccaaatcc
     9001 gccattccac aagcgttgcc aataccgaca ccagcgtaat ccacatcatt tcaataaccc
     9061 gcaatatgaa taaccgaagc caaagcggct tggcaaaaaa ggtcgacaac gtattttcac
     9121 tggagaaaaa cagatcgatt tcctgcaaaa aaaacctgcc cagaaaagcg ggccgcttgg
     9181 agaagcgcag aaaaaacaga ttaatcgcca acactacgcc acaaagaatg agcaatgcaa
     9241 ttatcgattt aaacgttaac cactgcccct ctgtcacaaa agaatcactg ttacgcagcg
     9301 ggctgatagt aagcaggtta acaaccgttg agatgcacac cgaaagcagc acatagcgag
     9361 tcagactttt atattttact tttttcgcta ctgaaatagc gacctgcttg acataactgg
     9421 tgcggatcat ccgaaacagc agaaagatga ccaacggccc aagtaacgaa atcaggaaca
     9481 gcgcatataa cgagggtaga agctggtata gcccccaact cattgcactc aacatgatta
     9541 acgacagaaa tcctgccacc agaggttgtg taagaaataa ttctattttg tggttctgct
     9601 ttttcttaaa ccacgaaaaa tcgatattct tcgtgacgca tttataagcc cagtacgcct
     9661 tgagatcaaa aataaaatta tacagcacca gggccagaat cccactaact acaccaatat
     9721 cctctgatgg aaaagtaaac cctgcatttt tccaataaaa gcctatcaga ataagtccaa
     9781 tagcataaat gtatatcaga tttcgtttat cctgaaagcg aataaaagtt aaataatcag
     9841 gaatcatttg aatacctttg catcactaaa tgctttacga caatgcataa ctcgttgctg
     9901 atccacgcca gcaaaatcat ttaacatatt ccccaaatag ggcttattac ttatttcaag
     9961 gaaagctacc ggggttggca tattcagaat atgtatcgag agattcagtc catttcaccg
    10021 tatgagttaa gtgtaatgag agattttctt tgataattga cggagcgacc tcgctttttg
    10081 ctgttacgtt catcagaacc tgatgctctg gcgaagcgat atccagtccg gcaagataat
    10141 cgcgcattgc ctgaacgccg tcttccatca aacgcgtgtg ccaggcaccg ctcacaccga
    10201 gtttaaccgg ttcgtatcca gcggccatca gcagcgtggt aaattcattc aacgcagcct
    10261 gcgttccccc aataacctgc tggcgcggcg tgttatcaca gctaatatcc agcgcaatgc
    10321 ctgattccgt aatcatcgtc tgcagttgtt cgcggttgat gcctttaacc gcctgatctt
    10381 acccagcaat agtggacacg cggctaagtg agtaaactct cagtcagagg tgact
//
LOCUS       DAETQL010000122        10399 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.122, whole genome shotgun sequence.
ACCESSION   DAETQL010000122 DAETQL010000000
VERSION     DAETQL010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.122"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            415..546
                     /locus_tag="K0W65_003583"
     CDS             415..546
                     /locus_tag="K0W65_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HBI3096691.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            806..1801
                     /gene="pgl"
                     /locus_tag="K0W65_003584"
     CDS             806..1801
                     /gene="pgl"
                     /locus_tag="K0W65_003584"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBI3096692.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1842..2795)
                     /locus_tag="K0W65_003585"
                     /pseudo
     CDS             complement(1842..2795)
                     /locus_tag="K0W65_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2979..4031
                     /locus_tag="K0W65_003586"
     CDS             2979..4031
                     /locus_tag="K0W65_003586"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HBI3096693.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            4107..5540
                     /locus_tag="K0W65_003587"
     CDS             4107..5540
                     /locus_tag="K0W65_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HBI3096694.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5723..7903
                     /locus_tag="K0W65_003588"
     CDS             5723..7903
                     /locus_tag="K0W65_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBI3096695.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(8044..9327)
                     /locus_tag="K0W65_003589"
     CDS             complement(8044..9327)
                     /locus_tag="K0W65_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBI3096696.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9462..10343)
                     /locus_tag="K0W65_003590"
     CDS             complement(9462..10343)
                     /locus_tag="K0W65_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI3096697.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDLCEMKWSD
                     IVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLS
                     SGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMA
                     SQYRDDRGREWDKIEIK"
BASE COUNT         2546 a         2405 c         2597 g         2851 t
ORIGIN      
        1 gccataaggg aatgagtgtt atatcatgct ttctacccta gtaagtatat ttgctttttt
       61 cgtatatttg gttcatcttt ttttgatgtt ttacatattc acaatgcaga ggatttatac
      121 cagaggacat tagtcttgag taagatacta caacatctcc aactatttta gccagcctga
      181 ctacttgact tggtaatatt tattgctaat aatcatgtat aaaataagct tatatacata
      241 gtaacttcag caaaggattt agtaatgatc aaaatcatat ctcaatgtac tgtttttttc
      301 tttaaaaaaa cagtacattc tattttataa tgactgtgta ttcccatgga aggagatcac
      361 ccaattctcc cccccgactt ttgacgcagt gtcttgttaa cagggggatt gaccatgctt
      421 attggctatg tacgcgtatc aacaaatgac cagaataccg atctacaacg taatgcgttt
      481 atttgtgccg gaagtgagtt gattgttgcg cgaatgggaa gttggccaga agttgttatg
      541 gcatagtgtt tacggtcgtt gatacaggta aacaaaagcg gggagagtca tactccctga
      601 atagtcgcga aaatatctgt ggcattgtca aattcgtgtc ggtgactaat attttagtaa
      661 tatgccattc gaaatggagt gctaacttat tgatttaatt gatgtgcgat attgtatttt
      721 atcgtgtttt gttgtctgtt ttttatccag taagtgttgt tatatgtatg aaagataagg
      781 gatttatcat ttttgaggtg tgttcatgaa gcaaacagtt tatatcgcca gccctgagag
      841 ccagcaaatt cacgtctgga acctgaatca tgaaggcgca ctgacgctga cacaggttgt
      901 cgatgtgccg gggcaggtgc agccgatggt ggtcagcccg gacaaacgtt atctctatgt
      961 tggtgttcgc cctgagtttc gcgtcctggc gtatcgtatt gccccggacg atggcgcact
     1021 gacctttgcc gcagagtctg tgctgccggg tagtccgacg catatttcca ccgatcacca
     1081 ggggcagttt gtctttgtag gttcttacaa tgcgggtaac gtgagcgtaa cgcgtctgga
     1141 agatggcctg ccagtgggcg tcgtcgatgt agtcgagggg ctggacggtt gccattccgc
     1201 caatatctca ccggacaacc gtacgctgtg ggttccggca ttaaagcagg atcgcatttg
     1261 cctgtttacg gtcagcgatg atggtcatct ggtggcgcag gaccctgcgg aagtgactac
     1321 cgttgaaggg gccggcccgc gtcatatggt attccatcca aacgaacaat atgcgtattg
     1381 cgtcaatgag ttaaacagct cagtggatgt ctgggaactg aaagatccgc acggcaatat
     1441 cgaatgtgtc cagacgctgg atatgatgcc ggaaaacttc tccgacaccc gttgggcggc
     1501 tgatattcat atcaccccgg atggtcgcca tttatacgcc tgcgaccgta ccgccagtct
     1561 gattaccgtt ttcagcgttt cggaagatgg cagcgtgttg agtaaagaag gcttccagcc
     1621 aacggaaacc cagccgcgcg gcttcaatgt tgattacagc ggcaagtatc tgattgccgc
     1681 tgggcaaaaa tctcaccaca tctcggtata cgaaattgtt ggcgagcagg ggctactgca
     1741 tgaaaaaggc cgctatgcgg tcgggcaggg accaatgtgg gtggtggtta acgcacacta
     1801 accgctgatt taaccggcgc agtctctcct gcgccggtgt attaacttat ctcctgtaac
     1861 gcatgtctct ggcgttcgac gatattggtc cacaaattgt ctttatcgtc agtccacaaa
     1921 ttaatcagca aggaaaaaaa gcgttcagct gccggagaaa gcacggcatc tttgcggcga
     1981 ataatcccca atgtccgacg tataacgggt tccaccagtg ggataccaat aatcgaagaa
     2041 tagggcgcat ggggcattgc cagaccagga agcgccgaaa tccccagtcc tgcctccact
     2101 agtcctaatg acgtcgaaag atgacgcact tcgtaaaacc agtccagctt ccagggcttg
     2161 tcggctagct gttgttctat caacaatcgg ttgccgctgg aggaacgtac gccaatcatt
     2221 ttgtaaccca ctaactcctg ccattctaca agctactttt tggccaatgg atggtcacgc
     2281 cgacaggcca gcacgaatgg ctcgttgacc agtggggtaa aatcaatgga tgaatttgtg
     2341 acgttgttca tgtttatgcc aaagtctgat tcgttacaca gcaccgattc catgcaatta
     2401 ttcgtgcctt gttccagaat ccgcacttta atattgggat acagctcatt aaatttaccg
     2461 attgccagcg gtaaaaaata aaatactgcg gtcggaatac acgctaatgt caccatacca
     2521 cgatgataag cattcatatc acgaatatta aaaagcgttt catcgaattt ctttattaat
     2581 tcccgcgcct ccgggagcaa cctttttcct gcttttgtta acgtaacctt gcgagttgta
     2641 cgctcaaata gttgaatatg taaatcttcc tccatctttt ttattcttcg cgttaatgca
     2701 ggttgcgtaa tattgagtaa tttagcggca ttgttaaatg aactggactc tgccagtatg
     2761 acaaatgcct tcatacttga taattcatgc ttcattatta ctccggaaaa tggaagcgac
     2821 gattttgggg ggctggtcgt taaaaatttt aactgcattt agccaactta aattaatgaa
     2881 aaaatgttat taatcgttga gctaaagtca ttagagatgc tttgtcctta atgtaaccat
     2941 atcgcaataa gttatgtttt taaattgagg gcattattat gaaaaaaata ccctgcgtga
     3001 tgatgcgagg tggaacctcg aggggcgcgt tcctgttagc ggaacattta cccgaagatc
     3061 aaacgcagcg cgataaaata ttgatggcaa ttatgggttc cggtaacgat ctggaaattg
     3121 acggtattgg cggcggtaat ccactgacca gtaaagtcgc cattattagc cgttccagcg
     3181 atccgcgtgc tgatgtcgat tatctgtttg ctcaagttat cgtccatgag caacgtgtcg
     3241 ataccacgcc taactgcggc aatatgctgt ctggcgtagg ggcattcgcc attgaaaatg
     3301 gtttgattgc agcgacttcg ccagttaccc gtgtacgtat ccgcaacgtc aatacgggta
     3361 cgttcatcga agctgatgtg caaacgccaa atggtgttgt cgagtacgag ggtagcgcca
     3421 gaattgacgg cgtaccgggt actgccgcac cggttgcgct cactttcctg aatgccgctg
     3481 gaacgaaaac tggaaaagtt ttcccgactg ataatcagat tgattatttt gacgatgtcc
     3541 cggtgacctg tatcgatatg gcgatgccag tcgtcattat tccggctgaa tatctgggaa
     3601 aaacaggtta tgaattaccg gcggaactgg atgccgacaa agcattatta gcccgcattg
     3661 aatctatccg gctacaagcg ggtaaagcaa tgggcttagg tgatgtcagt aatatggtta
     3721 tccctaaacc tgtgcttatt tctccagcgc agaaaggcgg ggcaattaat gtgcgttatt
     3781 ttatgccgca ttcttgccat cgcgcgctgg cgataaccgg tgctattgct atttccagta
     3841 gttgtgcatt ggaaggcacc gtcacccgac aaatcgtccc ttctgtagga tatggcaata
     3901 tcaatattga acaccccagt ggtgcgctcg acgttcattt aagtaatgaa ggtcaggatg
     3961 ccacgacgtt acgcgcatct gttattcgga caaccagaaa aatattttcc ggtgaagttt
     4021 atcttccctg aaaaaattcg ttgtcaggat aaggacaatc aataaaggac ttctgtatga
     4081 gtcatacaga aagaacagga ttttaaatga ataagaaatc gttatggaag ctaattctga
     4141 tattagcgat cccatgtatt attggtttta tgccagctcc ggcaggatta agcgaactgg
     4201 cgtgggtgct ttttggtatt tacctggcgg ccattgtggg tctggttatc aagcctttcc
     4261 cggaacctgt cgtactgtta attgccgttg ctgcctcaat ggtggtggtc ggtaacttat
     4321 ccgacggtgc gtttaagacc accgccgtat taagcggtta ctcttcaggt actacctggc
     4381 tggtgttctc ggcgtttacc ttaagcgccg catttgtgac caccggttta ggtaaactta
     4441 ttgcctatct gctgattggt aaaatcggta acaccatgct gggtctgggt tacgttacgg
     4501 tattcctcga tctggtactg gctccggcaa caccgtctaa caccgcgcgt gcgggcggca
     4561 ttgtgttacc gatcatcaac agcgtggcgg tggctttggg gtccgaaccg gaaaaaagtc
     4621 cgcgtcgtgt cggacattac ctgatgatgt ccatttacat ggtcaccaaa accaccagct
     4681 atatgttctt taccgcaatg gcggggaaca ttctggcgct gaaaatgatc aacgacattc
     4741 tgcacctgca aattagctgg ggtggatggg cgctggccgc cggattgccg ggcatcatta
     4801 tgctgctggt caccccgctg gtaatttaca ccatgtatcc accagaaatt aaaaaggtgg
     4861 ataacaaaac catcgctaaa gcgggccttg ccgaactggg accgatgaaa atccgcgaaa
     4921 aaatgctgct cggtgtcttt gtgctggcgc tactgggctg gattttcagt aagtctctgg
     4981 gggttgatga atccactgtg gcaatcgttg ttatggcgac catgctgctg ctgggtatcg
     5041 ttacctggga agacgtggtt aaaaataaag gcggctggaa taccttaatc tggtacggcg
     5101 gtattatcgg cttaagctcc ttattatcga aagttaaatt tttcgaatgg ttagctgaag
     5161 tctttaaaaa taacctggca tttgatggtc acggtaacgt tgctttcttc gttattattt
     5221 tcctcagcat catcgtgcgt tatttcttcg cttccggtag tgcctatatc gttgctatgt
     5281 tacctgtatt tgccatgctg gcgaacgtct ccggcgcacc gttaatgtta accgcgctgg
     5341 cactgttgtt ctccaactcc tatggcggca tggttactca ctatggcggc gcggcaggtc
     5401 cggtcatctt tggcgtgggt tacaacgata ttaaatcctg gtggttggtc ggtgcggtac
     5461 tgacgatatt aaccttcttg gtgcatatca ccctcggcgt gtggtggtgg aatatgctga
     5521 tcggctggaa catgctgtaa atatactcgt catacttcaa gttgcatgtg ctgcgtctgc
     5581 gttcgctcac cccagtcact tacttatgta agctcctggg gattcactca cttgtcgcct
     5641 tcctgcaact cgaattattt agagtatatc catttattat ctttctgcgc acttcacggt
     5701 gcgcagatat ctggagcatt tgatgatcaa gttatctgaa aaaggcgtgt ttctcgccag
     5761 taataacgaa ataattgccg aagaacattt caccggcgaa attaaaaaag aagaagcaaa
     5821 aaaaggcact attgcctggt ctattctctc ttctcataat acgtccggaa atatggataa
     5881 acttaaaatt aagtttgatt cattagcctc tcacgatatt acctttgttg gtattgtaca
     5941 gaccgctaaa gcgtccggaa tggaacgttt cccgctgccg tatgtgctga ccaactgcca
     6001 taactcactc tgcgccgtcg gcggcaccat taacggtgat gaccatgttt ttggtttatc
     6061 ggcagctcag cgttatggcg gtatttttgt gcctccgcat attgcggtca tccatcaata
     6121 tatgcgtgag atgatggctg gcgggggcaa aatgatcctc gggtcagaca gccacacccg
     6181 ttacggtgca ttagggacaa tggcagtcgg tgagggtggc ggtgagttgg taaaacagct
     6241 gcttaatgac acctgggata tcgactatcc gggcgtggtt gcggtgcatc tgaccggaaa
     6301 acctgcgccg tatgtggggc cacaggatgt ggcgctggct atcattggcg cggtgttcaa
     6361 aaacggttac gtcaaaaaca aagtcatgga gttcgttgga ccgggcgtta gcgcgctctc
     6421 taccgatttc cgtaacagcg ttgacgtgat gaccacggaa accacctgtt taagctccgt
     6481 ctggcaaact gatgaagaag tgcataactg gctggcgctg cacggtcgcg gtcaggatta
     6541 ctgccagctt aaccctcaac cgatggcgta ctacgatggc tgcatcagcg ttgatttaag
     6601 cgccatcaaa ccaatgattg cgctgccgtt ccacccgagc aacgtgtatg aaatcgacac
     6661 actgaaccag aacctgaccg acattctgcg tgagattgaa attgagtccg aacgcgtggc
     6721 gcacggtaaa gccaaactct cgctgctgga taaagtggaa aatggtcgcc tgaaagtgca
     6781 gcaggggatt atcgcgggct gttctggcgg taactacgaa aacgtcatcg cggcggcgaa
     6841 tgcactgcgc ggtcaatcct gtggcaatga caccttctcg ctggcggttt acccgtcatc
     6901 acagccggtg tttatggatc tcgccaaaaa aggtgtggta gcagatttga ttggcgcagg
     6961 cgcaatcatc agaaccgcat tctgtggccc atgctttggc gcgggcgata cgccaatcaa
     7021 caacggtttg agtattcgcc acaccacgcg taacttcccg aaccgcgaag gctctaagcc
     7081 agccaatggg cagatgtcag cggtggcgtt gatggacgct cgttctatcg ctgcgactgc
     7141 ggcaaacggt ggctatttaa cctctgccag cgaacttgat tgctgggaca acgtgccgga
     7201 gtacgccttc gatgtaacgc cgtataaaaa ccgtgtttat cagggctttg tgaaaggggc
     7261 aactcagcaa ccgctgattt acggaccgaa cattaaagac tggccggaat tgggtgcgct
     7321 gactgacaat atcgtcctga aagtgtgctc gaagatcctc gacgaagtca ccaccaccga
     7381 cgaactgatt ccttccggtg aaacctcttc ttatcgttca aatccgattg gtctggcggg
     7441 gaatgtcagc gagctgacag aggtgtttgc gcgcattaag cagattgctg gtcaggagca
     7501 tattgatccg ctgcaaactg aaattggcag catggtctat gcggtgaaac caggcgatgg
     7561 ttctgcgcgt gaacaggcgg cgagctgcca gcgtgtgatt ggcggtctgg cgaatattgc
     7621 cgaggagtac gcgactaaac gctaccgttc taacgtcatc aactggggga tgttaccgct
     7681 gcagatggcg gaagtgccaa cctttgaagt gggggattac atttacatcc ctggcattaa
     7741 agcggcgctg gataatccgg gtacgacgtt taaaggttat gtgatccatg aagatgcgcc
     7801 ggtaacggaa attacgcttt acatggaaag cctgacggct gaagagcgcg agattatcaa
     7861 ggcgggtagt ttgattaact tcaataaaaa ccgtcagatg taaaaagcgc catgtgaatg
     7921 taggtcgcat tcggcactta ttgtcggatg cgatgctggc gcatcttatc cgacctacaa
     7981 atcgcatcga accgtaggcc ggataaggcg tttacgccgc atccggcaaa tagttaatag
     8041 ctcttacttc ttctcctctg caaccacttt gctgcccacg ccgcggttat tgtattccca
     8101 catgcggttg tagttagtgt cattcagatt gcgctgtatt tcgtcgttat catctacgct
     8161 gccggtatta cccgcaaacg gacgattaga gatcaccgca tcggcccacg gtttagccgt
     8221 gttaaaacct tcgttgatgg cgctatcacg gatcaccacc tgaccgttgg tattagcatc
     8281 aacatccagc gagcggccca gttgcgccac gccatcaccg gaagcattga aacggctgtt
     8341 tacggcgagg aaaccgtaat agatgttaga cagcgtagcc ggtgcaaaca catatgcttc
     8401 ttgctgggta cgtgagttca ccacgcggaa ttcggtgtta tcgaacacca ctgcgccgcg
     8461 accagaaacg atatccacat ccccttcaat atagctgttg gtcaccagcg tacgcggctg
     8521 acggttggtt tccagacggt tctgcacacc gctgttggtg acaaagaagg tgttctgacg
     8581 accgagaatg ttaacgttgt taatctgtac ttggtcaccg tcagtacgca gtgctaccgc
     8641 cggatggtta cctgcatcta cgctatcgcc cagcgtgttt tcgatggtca gattttgcag
     8701 ttgcagtcca ttgttttgtg accagaagac cgcagaacac agaacaccga tactgtcgct
     8761 gcgtttgctc tggcagctat cgtacatata ccacgctggt ttacctggca tatatttgcc
     8821 gcgcgggttg acgtcgtgac gccagtcggc agggctcatg cccccatcaa gggaaagccc
     8881 aatcttcaca tcaatcggtt tttcacctgt accgtacaga gtgattccac ctggagcagc
     8941 agggacatac accgttccct gatactcacc aggcatcacg gcaatatact ggcgcttgtt
     9001 ggtgcgcttg ataattgccg catctaccgc cgcctgaatc gtggtatgcg ttacaccttg
     9061 agtacccgcc gggccgacaa caaagtcagg ttgcgcaggc agggttatcg gggacggatt
     9121 ccacgctgca gcacctggtg tcagggatgc aaaatagtgt tgcgcatcga aattcagcgc
     9181 ttcttttgcc gacagaatcg ggcgagaaga ggtaccaggc gcagtttgat cagaaggacg
     9241 ttgatcgggt ggtgttgagc tacaggcggt cagcgtcacg ccaaaagcca atgccagcgc
     9301 cagacgggaa actgaaaatg tgttcacagg ttgctccggg ctatgaaata gaaaaatgaa
     9361 tccgttgaag cctgcttttt tatactaagt tggcattata aaaaagcatt gcttatcaat
     9421 ttgttgcaac gaacaggtca ttatcagtca aaataaaatc attatttgat ttcaattttg
     9481 tcccactccc tgcctctgtc atcacgatac tgtgatgcca tggtgtccga cttgtgcccg
     9541 agaagatgtt gagcaaactt atcgcttatc tgcttctcat agagtcttgc agacaaactg
     9601 cgcaactcgt gaaaggtagg tggatcccct tcgaaggaaa gacctgatgc ttttcgtgcg
     9661 cgcataaaat accttgatac tgtgccggat gaaagcggtt cacgacgagt agatgcaatt
     9721 atggtttctc cgccaagaat ctctttgcat ttatcaagtg tttccttcat tgatatcccg
     9781 agagcatcaa catgcaatgt tgtagggatg gcaattttta cgcctgtttt gctttgctcg
     9841 acataaagat atccatctac gatatcagac cacttcattt cgcataaatc accaactcgt
     9901 tgcccggtaa caacagccag ttccattgca agtctgagcc aacatggtga tgattctgct
     9961 gcttgataaa ttttcaggta ttcgtcagcc gtaagtcttg atctccttac ctctgatttt
    10021 gctgcgcgag tggcagcgac cgggtttgtt gttatatggc cttcagctat tgcctctcgg
    10081 aatgcatcgc tcagtgttga tctgattaac ttggctgatg ccgccttgcc ctcgtctatg
    10141 tatccattga gcattgccgc aatttctttt gtggtgatgt cttcaagtgg agcatcaggc
    10201 agccccctcc ttattgcttt aattttgctc atgtaattta tgagtgtctt ctgcttgatt
    10261 cctctgctgg cgaggatttt ttcgtagcga tcaagccatg aatgtaacgt aacagaatta
    10321 tcactgttga ttctcgctgt cagaggcttg tgtttgtgtc ctgaaaataa ctcaatgttg
    10381 gcctgtatag cttcagtga
//
LOCUS       DAETQL010000123        10087 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.123, whole genome shotgun sequence.
ACCESSION   DAETQL010000123 DAETQL010000000
VERSION     DAETQL010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10087
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.123"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..123)
                     /locus_tag="K0W65_003591"
     CDS             complement(<1..123)
                     /locus_tag="K0W65_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3096698.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQL"
     gene            complement(342..1082)
                     /gene="pflA"
                     /locus_tag="K0W65_003592"
     CDS             complement(342..1082)
                     /gene="pflA"
                     /locus_tag="K0W65_003592"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBI3096699.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1274..3556)
                     /gene="pflB"
                     /locus_tag="K0W65_003593"
     CDS             complement(1274..3556)
                     /gene="pflB"
                     /locus_tag="K0W65_003593"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBI3096700.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3611..4468)
                     /gene="focA"
                     /locus_tag="K0W65_003594"
     CDS             complement(3611..4468)
                     /gene="focA"
                     /locus_tag="K0W65_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBI3096701.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4874..6634)
                     /locus_tag="K0W65_003595"
     CDS             complement(4874..6634)
                     /locus_tag="K0W65_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HBI3096702.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6764..7390
                     /locus_tag="K0W65_003596"
                     /pseudo
     CDS             6764..7390
                     /locus_tag="K0W65_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7589..8677
                     /gene="serC"
                     /locus_tag="K0W65_003597"
     CDS             7589..8677
                     /gene="serC"
                     /locus_tag="K0W65_003597"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBI3096703.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8748..10031
                     /gene="aroA"
                     /locus_tag="K0W65_003598"
     CDS             8748..10031
                     /gene="aroA"
                     /locus_tag="K0W65_003598"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBI3096704.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2573 a         2411 c         2529 g         2574 t
ORIGIN      
        1 tagttgccat gttttacggc aatgagagca gagatagcgc tgatgtccgg cagtgctttt
       61 gccgttacgc accacgcctt cagtagcgga gcaggaagga catctgatgg aaatggaagc
      121 cacgcaagca ccttaaaatc accatcatac actaaatcag taagttggca gcattaccgg
      181 tattcgatgg ggaagaactt cacgcgagct attatcagtc gttattatct caggtacggg
      241 atatggtaga tgcactataa gatgtgttaa aaacgctgta gcagaatgaa gcgcggaata
      301 aaaaagcggc aactcaataa agttgccgct ttacgaggaa attagaacat taccttatga
      361 ccgtactgct caagaatgcc tttcacgcgt tccatggtct ctttcttcgg tggtttaaca
      421 ccatcgagtt tgtattcttc acccatcgcc acccatttgt gtttgcccag ttcgtggtag
      481 gggagaagtt cgattttctc aacgttaccc atatcacggg taaattcacc gaggcgatgt
      541 gccgaatcgt catcgtcaga ccagcctggg acaacaacgt agcggatcca caccttcaca
      601 tttttgttcg ccagatattt agcgaactcc agcgtgcggt ggttggaaac tccaaccaga
      661 ttttggtgga tctcgtcgtt catctgtttg agatcgagca ttaccaggtc ggttacttcc
      721 agcagttcat caatcaccgg atcgtaacga cgaacaaaac cgttggtatc cagacaggta
      781 tgaatgcctt cttttttaca ggcgcggaac cagtcacgaa caaactcagc ttgcaggatt
      841 gcttcaccgc cggatgcggt aacgccgccg ccggaagcgt tcataaagtg gcgataggtc
      901 accacttcct tcatcaaatc ttcaacggta acttctttac cgccatgcgt gtcccaggtg
      961 tcgcggttat gacaatacag gcagcgcatc aggcagccct ggaaaaaggt gataaagcga
     1021 atacctgggc cgtctacggt tccacaggat tcaaaggagt gaatgcgacc aataactgac
     1081 attgcggtgt ttctccagat gtggcccatc tgaggccgtg ttggtgcgca gctcgaaggc
     1141 tacgtcgagt ctgttttggc agtcacctta aagtatagat agctgacaaa aaaggctctc
     1201 gtgctaaaaa aggccccact ttcgtggagc ctttattgta cgctttttac tgtacgattt
     1261 cagtcaaatc taattacata gattgagtga aggtacgagt aataacgtcc tgctgctgtt
     1321 ctttagtcag cgagttgaaa cgtactgcgt agccagatac acggatggtc agctgcggat
     1381 atttttccgg gttttccatc gcgtcgagca gcatttcacg gttcatcacg ttaacgttca
     1441 ggtgctgacc accttcgatg gatgcttcgt ggtggaagta accatccatc agaccagcca
     1501 ggttggtctt acgaacttcg tcgtctttac ccagtgcgtt cggaacgata gagaaggtgt
     1561 aggagatacc atctttagcg taagcaaacg gcagtttagc aacggaagtc agagacgcta
     1621 cagcaccttt ctggtcacga ccgtgcatcg ggttagcacc cggtccgaac ggcgcgccag
     1681 cacgacgacc gtctggggtg ttaccagttt tcttaccata cacaacgtta gaagtgatgg
     1741 tcagaacaga ctgagtcggg atagcgtcac ggtaggtgtg cagtttctga attttcttca
     1801 tgaaacgttc taccaggtca acagccaggt catctacacg cggatcattg ttaccaaact
     1861 gcgggtattc gccttcgatt tcgaagtcga tagccagacc gtcttcgtca cgaatcggtt
     1921 taactttcgc atatttgatt gcagacaggg agtcagcagc aacggacaga ccagcgatac
     1981 cacacgccat ggtgcggata acgtcacggt cgtgcagcgc catcagagag gcttcgtagc
     2041 tgtacttgtc gtgcatgtag tggatgatgt tcagtgcagt gatgtactgt ttagccagcc
     2101 agtccatgaa gtgatccatg cgctccatca cttcatcgta gctcaggaga tcgcctttga
     2161 tcggttcaga cttcggacca acctgcattt tcagtttttc gtcaacgccg ccgttgattg
     2221 cgtacagcat ggttttcgcc aggtttgcac gcgcaccgaa gaactgcatt tgtttaccaa
     2281 cgatcatcgg gcttacgcag caagcgatag cgtagtcatc gttgttgaag tccggacgca
     2341 tcaggtcatc gttctcatac tgcagagaag aggtgtcgat ggacacttta gcggcgaatt
     2401 tcttgaagtt cagcggcagt ttttcagacc acagaatggt catgttcggt tccggagacg
     2461 gccccatagt gtacagggtg ttcaggaaac ggaagctgtt tttggtaacc agggtacgac
     2521 catcgaggcc cataccaccg atagattcgg ttgcccagat tgggtcgcca gagaacagtt
     2581 catcgtattc cggagtacgc aggaagcgaa ccatacgcag tttcatgacc aggtggtcaa
     2641 ccatttcctg cgcttcttgt tcggtgatct tgccagcttt caggtcacgt tcgatgtaca
     2701 catccaggaa ggtggaggta cgaccgaagg acattgcagc accgttctga gacttaacag
     2761 cagccaggta gccgaagtaa gtccactgga tagcttcctg agcgttggta gccggaccag
     2821 agatgtcgta gccgtatttc gcagccattt ctttcatctg acccagagcg cggtgctgtt
     2881 cagcgatttc ttcgcgcaga cggatagtct gttccaggtt tacgccgttt tccagatcag
     2941 cctgtagaga ggtgaactga gcgtatttgt ctttcatcag gtagtcgata ccgtacagcg
     3001 caacgcgacg gtagtcaccg atgatacggc cacggccata agcatctggc agaccggtca
     3061 gaacaccgga tttacggcaa cgcaggatgt ccggagtgta aacgtcgaac acgccctggt
     3121 tgtgagtttt acggtattca gtgaagattt ttttgatcat cgggtccagt tcgcggttgt
     3181 acgctttgca ggaaccttcg atcattttga taccaccgaa cgggataaga gcacgtttca
     3241 gcggagcttc agtctgcaga ccaacaactt tttccaacgc tttattgatg tagccagcgt
     3301 cgtgagaggt gatggtggaa gcaacagcgg tgtcaaaatc aactggcgcg tgagtgcggt
     3361 tttccagttt aacaccttcc attactttgt cccacagggt ggtggtcgct tcagtagcgc
     3421 cagccaggaa ggactcgtca ccctcgtacg gagtgtagtt tttctgaatg aagtcacgga
     3481 cgtttacttc attctgccag tcacctttgg taaaaccttc ccaggctgtg gctaactttt
     3541 cattaagctc ggacatgtaa cacctacctt cttaagtgga ttttttattt actgcgtact
     3601 tcgacaacca ttaatggtga tcgttttcac gcaggtaaat gacccagtat gtcaacccaa
     3661 ccaacaaacc accgccgata atattaccga tcgtaaccgg gatcaggtta tcagtgatga
     3721 agttcatcac ggtcaggtga gaaaaatttt ccggtgcaga accgacagcg gtccagaatt
     3781 ccggagatgc gaagtcgcgg attacaatac ccatcgggat cataaacatg tttgcgatac
     3841 tgtgctcaaa accgctggca acaaacatcg cgaccggcag caccataatg aacgctttgt
     3901 ccatcaggct gcggccagaa taactcatcc atactgccag acataccatc aggtttgcca
     3961 ggataccaag acagacggcc tcaataaaag tatggtgcac tttgtggtcg gcggtttgta
     4021 ggacgtttag tccccattga ccatttgcgg tcatatactc gccggaaagc cacattaaaa
     4081 gtacaaacag cagtgcgccg accaggttgc caaaatagac atttagccag tttttagcca
     4141 actgacacca ggtgatgcgc ccactcgcct tagcaacaac aatcaacact gtggaagtaa
     4201 agagatcggc tccgcagaca acacaaagaa tcagccccag agagaagcaa atgccgccaa
     4261 ccagttttgc catgccgaag ggcattgtgc ctgtgccagt ggttgctgtg atatagaaga
     4321 cgaatgcgat tgagatgaaa acaccggcgg taatcgccag atagaaagtc ttaagcggat
     4381 gtttcgttgc tttatagaca cccgcctctt cggccacttt ggccattgca gcaggaagta
     4441 aaagatcaaa agggttgtca gctttcacac taactctctc tttattaagt cggcgacgag
     4501 atactaacaa agcattatag atgagaaatt gatatagatc atatctcgcc tggcttatag
     4561 gcccgtaact cgcatggttt ttatgcaaat acggagtaac tatttgatta tccaaataaa
     4621 aataaatttt aaaaattaac aaatgagttg aattttttcc gcatcctccg ctaaaacagt
     4681 taattaaaag ggagcatcag gcgaataaag taacaatatc gatcgtattt attaaataca
     4741 aattaccgat atttaacctt ataattacaa ttattttatt aatgcaaata tatgtaaagc
     4801 ggggcattaa aaaaacgccc cgtaatataa ctcagactaa tcattaagcc tacattgcgt
     4861 aggctatttg attttatttt gcccagaatg ctgctttggc gcgctgcagc ttttcgtagg
     4921 ccttcaacaa cgactgatgt gcagcaaacg cgtgcagatc gctatctacc ggttgcaggc
     4981 cgtaaaacgc cgcttcgccg ctcattgccg cactggcggc ttctacggca tctgcgccgt
     5041 acatgcgaac aaacgcattc agatattgca gcggctggcg atcttcttcc tgtgccagta
     5101 ataacaacgt ttgcaggcag cgataatagt tggcgcgttc cggactaaat actgatgagt
     5161 taaactccat cgtccattcg gtccagacca gagcctgttc cagatcgcca ccagccagcg
     5221 ccagcatggc ttttaattca ccgatacgca gggtgtacca accgttatcc gacccggtcg
     5281 ccagacccaa cagctcacgc acgcgggtaa agtcatcaaa accttcttca tccagttgct
     5341 cgatgaggtt caggtaatct tctttttccc actcgctgcc tggtagcgaa agaatcgttt
     5401 cacgtaaatg gctgcccata ctgttattcg cgagccacag atcttcagcc ggataaatat
     5461 cggacatgcc aggcacgata atacggcaag cataaacgcc cagatgctcg taatcggcaa
     5521 tataaacttc tttatcttct ttgttgaaga tagccatcag cgtagcaaac tcttcttccg
     5581 tggtgccgga gaaattccag tccacaaacg gataatcggc atcctgcttg aacaggtccc
     5641 aggagattaa accgctggaa tcgataaagt gcgtttcgag gttggtatgt tcagcgactt
     5701 cttcatcatc gaaggttggc ggagtaaaca catccaaatc tttcaggcca cgaccttgca
     5761 gcagctcggt cacggtacgt tccagtgcta cgccaaaatc aggatgcgca ccgaaagagg
     5821 caaagcaggt gccgtttgca ggattaaaca gtaccacgca aatcaccgga tactggccgc
     5881 caagggaacc atcatatgcg aagatcggga aaccttctgt ttccagtgtt tcgatcgctt
     5941 caaccaccgc tgggtaacgc gccagcacgt ctgccgggat ttccggcaag ctgatgcttt
     6001 cagcaataat gcggtttttc acgtagcgtt cgaaaacttc ggacaacccc tgaacgcgtg
     6061 cttcgttgcg ggtattgcca gcggacatac cgttagaaac gtacaggtta ccaatgatat
     6121 tcatcggaat ataaacggtc tgattgtcgg actggcgcgt aaacggcaga ccgcaaatac
     6181 cacgatcttc gttaccggat tgtaggtcaa tcagcatact gccggtcaat tcattctccg
     6241 gatcgtaaaa tgcgcgcaga cggtcatcga gcagcccttc tggcacatcg tcattttcgc
     6301 tcagtgggaa ccatttttcg ttgggataat gcacaaatgg accgttggcg atggtttcgc
     6361 ccagccagaa atcagcaaaa aagtagttgg ttgagagacg ctcgaaatat tcaccgagtg
     6421 cagaagccag cgccgctttc ttggttgcgc ctttaccgtt ggtaaaacac agtgcgcact
     6481 ctttgtcgcg aatatgtaca gaccagacgt taggcacggg attcagccag gaggcctctt
     6541 caatctgaaa gccgaggtct gaaagttttt gctggaagcg agcgatggaa tcttccagag
     6601 cggcatcttt gccggggata aatgtttgcg tcatgaaaat cactttagtc gtacggaaag
     6661 cgcgcaataa tacgggtttt atctcaaagg cgctatcacc gccgccatgc cgatgaactg
     6721 ttgactatgc ttttagcgga taaaccacgt taagagcata aaaatgaagg cgttcgatct
     6781 ccaccgtatg gcatttgata aagtgccttt tgatttcctt ggcgaagttg cactacgtag
     6841 tctttatacc tttgtactgg ttttttgttc ctcaaaatga ccggaagacg cggtgtgcga
     6901 cagatgtcgc tgtttgaagt gttaatcatt ctcacgctgg gttcagcggc gggagatgtg
     6961 gcgttttatg atgatgtgcc gatggtgccg gtacttatcg tctttatcac tctggcgttg
     7021 ttataccgcc tggtaatgtg atggcgagct ggcctggtcg aaactcaata actccaacat
     7081 gacggaattt gagttcttta tggagctacg attgcgtggc gtggagcagc tggggcaggt
     7141 acgtctggcg attctcgaaa ccaacgggca aatcagtgtc tatttctttg aagatgacaa
     7201 ggtgaaaccg ggtttactta ttttacccag tgattgtacg cagcgttaca aagtggtgcc
     7261 ggagtcggcg gactatgcct gcattcgttg tagtgaaatc attcatatga acgcggggga
     7321 aaaacaatta tgtccgcgct gtgcaaatcc agaatggacg aaggcaagtc gggcaaaacg
     7381 ggtgacctga cagtaaaaac atcggctttt tgctaataat ccgagagatt cttttgtgtg
     7441 atgcaagcca catttttgcc ctcaacggtt ttactcattg cgatgtgtgt cactgaatga
     7501 taaaaccgat agccacagga ataatgtatt acctgtggtc gcaatcgatt gaccgcgggt
     7561 taatagcaac gcaacgtggt gaggggaaat ggctcaaatc ttcaatttta gttctggtcc
     7621 ggcaatgcta ccggcagagg tgcttaaaca ggctcaacag gaactgcgcg actggaacgg
     7681 tcttggtacg tcggtgatgg aagtgagtca ccgtggcaaa gagttcattc aggttgcaga
     7741 ggaagccgag aaggattttc gcgatcttct taatgtcccc tccaactaca aggtattatt
     7801 ctgccatggc ggtggtcgcg gtcagtttgc tgcggtaccg ctgaatattc tcggtgataa
     7861 aaccaccgca gattatgttg atgccggtta ctgggcggca agtgccatta aagaagcgaa
     7921 aaaatactgc acgcctaatg tctttgacgc caaagtgact gttgatggtc tgcgcgcggt
     7981 taagccaatg cgtgaatggc aactctctga taatgcggct tatatgcatt actgcccgaa
     8041 tgaaaccatc gacggtatcg ccatcgacga gacgccagac ttcggcaaag atgtggtggt
     8101 cgccgccgac ttctcttcaa ccattctttc ccgtccgatt gacgtcagcc gttatggtgt
     8161 aatttacgct ggcgcgcaga aaaatatcgg cccggctggc ctgacaatcg tcatcgttcg
     8221 tgaagatttg ctgggcaaag cgaatatcgc gtgtccgtcg attctggatt attccatcct
     8281 caacgataac ggctccatgt ttaacacgcc gccgacattt gcctggtatc tatctggtct
     8341 ggtctttaaa tggctgaaag cgaacggcgg tgtagctgaa atggataaaa tcaatcagca
     8401 aaaagcagaa ctgctatatg gggtgattga taacagcgat ttctaccgca atgacgtggc
     8461 gaaagctaac cgttcgcgga tgaacgtgcc gttccagttg gcggacagtg cgcttgacaa
     8521 attgttcctt gaagagtctt ttgctgcagg ccttcatgca ctgaaaggac accgtgtggt
     8581 cggcggaatg cgcgcgtcta tttataatgc catgccgctg gaaggcgtta aagcgctgac
     8641 agacttcatg gttgagttcg aacgccgtca cggttaatgc cgaaattttg cttaatcccc
     8701 acagccagcc tgtggggttt ttatttctgt tgtagagagt tgagttcatg gaatccctga
     8761 cgttacaacc catcgctcgt gtcgatggca ctattaatct gcccggttcc aagagcgttt
     8821 ctaaccgcgc tttattgctt gcggcattag cacacggcaa aacagtatta accaatctgc
     8881 tggatagcga cgacgtgcgc catatgctga atgcattaac agcgttaggg ttaagctata
     8941 cgctttcagc cgatcgtacg cgttgcgaaa ttatcggtaa cggcggtcca ttacacgcag
     9001 aaggtgccct ggagttgttc ctcggtaacg ccggaacggc aatgcgtccg ctggcggcag
     9061 ctctttgtct ggatagcaat gatattgtgc tgaccggtga gccgcgtatg aaagagcgcc
     9121 cgattggtca tctggtggat gcgctgcgcc tgggcggggc gaagatcact tacctggaac
     9181 aagaaaatta tccgccgttg cgtttacagg gcggctttac tggcggcaac gttgacgttg
     9241 atggctccgt ttccagccaa ttcctcaccg cactgttaat gactgcgcct cttgcgccgg
     9301 aagatacggt gattcgtatt aaaggcgatc tggtttctaa accttatatc gacatcacac
     9361 tcaatctgat gaagacgttt ggtgttgaaa ttgaaaatca gcactatcaa caatttgtcg
     9421 taaaaggcgg gcagtcttat cagtctccgg gtacttattt ggtcgaaggc gatgcatctt
     9481 cggcttctta cttcctggca gcagcagcaa tcaaaggcgg cactgtaaaa gtgaccggta
     9541 ttggacgtaa cagtatgcag ggtgatattc gctttgctga tgtgctggaa aaaatgggcg
     9601 cgaccatttg ctggggcgat gattatattt cctgcacgcg tggtgaactg aacgctattg
     9661 atatggatat gaaccatatt cctgatgcgg cgatgaccat tgccacggcg gcgttatttg
     9721 caaaaggcac caccacgctg cgcaatatct ataactggcg tgttaaagag accgatcgcc
     9781 tgtttgcgat ggcaacagaa ctgcgtaaag tcggcgcgga agtggaagag gggcacgatt
     9841 acattcgtat cactcctccg gaaaaactga actttgccga gatcgcgaca tacaatgatc
     9901 accggatggc gatgtgtttc tcgctggtgg cgttgtcaga tacaccagtg acgattcttg
     9961 atcccaaatg cacggccaaa acatttccgg attatttcga gcagctggcg cggattagcc
    10021 aggcagcctg aatgaacaac gggcaataaa tagccaaatc tttctttatc aaaacgtcgg
    10081 cacattg
//
LOCUS       DAETQL010000124        10046 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.124, whole genome shotgun sequence.
ACCESSION   DAETQL010000124 DAETQL010000000
VERSION     DAETQL010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.124"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..60)
                     /locus_tag="K0W65_003599"
     CDS             complement(<1..60)
                     /locus_tag="K0W65_003599"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096705.1"
                     /translation="MASISIRCPSCSATEGVVRN"
     gene            119..457
                     /locus_tag="K0W65_003600"
     CDS             119..457
                     /locus_tag="K0W65_003600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096706.1"
                     /translation="MSSNNEYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLN
                     ARNQLDKNLLEMAMQMVYGKDMVTTSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYN
                     IDPLCECFKY"
     gene            627..1760
                     /locus_tag="K0W65_003601"
     CDS             627..1760
                     /locus_tag="K0W65_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096707.1"
                     /translation="MKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVG
                     PDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKS
                     KTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRS
                     VALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISV
                     RNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAK
                     DRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRV
                     LHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2439..2657
                     /locus_tag="K0W65_003602"
     CDS             2439..2657
                     /locus_tag="K0W65_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HBI3096708.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2875..3117
                     /locus_tag="K0W65_003603"
     CDS             2875..3117
                     /locus_tag="K0W65_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096709.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3447..4376)
                     /gene="fdhE"
                     /locus_tag="K0W65_003604"
     CDS             complement(3447..4376)
                     /gene="fdhE"
                     /locus_tag="K0W65_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBI3096710.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4373..5008)
                     /gene="fdoI"
                     /locus_tag="K0W65_003605"
     CDS             complement(4373..5008)
                     /gene="fdoI"
                     /locus_tag="K0W65_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBI3096711.1"
                     /translation="MKRRDTIVRYTALERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(5005..5907)
                     /gene="fdoH"
                     /locus_tag="K0W65_003606"
     CDS             complement(5005..5907)
                     /gene="fdoH"
                     /locus_tag="K0W65_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HBI3096712.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5920..8970)
                     /gene="fdnG"
                     /locus_tag="K0W65_003607"
     CDS             complement(5920..8970)
                     /gene="fdnG"
                     /locus_tag="K0W65_003607"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8383..8385),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBI3096713.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9164..9997
                     /gene="fdhD"
                     /locus_tag="K0W65_003608"
     CDS             9164..9997
                     /gene="fdhD"
                     /locus_tag="K0W65_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBI3096714.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2528 a         2426 c         2569 g         2523 t
ORIGIN      
        1 gttacgcacc acgccttcag tagcggagca ggaaggacat ctgatggaaa tggaagccac
       61 gcaagcacct taaaatcacc atcatacact aaatcagtaa gttggcagca ttaccggttt
      121 gtcgagtaat aatgaatatg acctgtttaa aaatagagaa acctcactgt ggaaacgcaa
      181 agtaccagaa agcgcagcgc tggcaatgac gggaacagta tataaattat ttggtaatcg
      241 cttactcaac gcccgcaatc agttagataa aaatctcctc gaaatggcta tgcaaatggt
      301 ttatggcaaa gatatggtga caacgtccgg ggatatattg attgatatac aattaaataa
      361 ggatggcagt ctccagagta agcactacac acttgatgta ggtgaagtta taaacagtta
      421 taatattgac cctctttgtg aatgcttcaa gtattaatca aacttactta catgataaaa
      481 atcctggtga cgttattagc ctgtatggca ctttagataa cattcccctt gtagttaagg
      541 aatgcacggg taaaaatcat caaattaatg ttaaagccaa attgccacaa gaacgaagtg
      601 aaaaagttga ggatatgtgt gaaagaatga aaggtggcat ctctatgttt aaccacacca
      661 caaaaacagc tgataatata gagcataatt tgcagctaag ttttctcgtg ggttcacgtc
      721 cgtcgatcac caataaatac tcagcaaaag atgtaggtcc agatatactt gcattgcccg
      781 tcaacgtata tcatgcggat tttaaaatta aaatcaataa tgcagtggag aaggcttcat
      841 caattgtgtt atgtgctgtg caaaaaaatc tccatgcagc aaggaaggtt cttagcggga
      901 taataggaaa tgtgccaaaa aataataaat caaaaacaat tatttttgat ttgcgttttc
      961 ttagtgcaat taagcagaaa gtttttttgg gcagtgaaaa accgttgttt gtaaagcata
     1021 ccgcaatgat gagggaggca tgtaaggaat taccgcagtc agtggaatat gttcctttag
     1081 cctgtgaagc gcatagccat cgcagcgtcg cccttgccaa aaccgtggct gcgcccatca
     1141 tggcgacagg tgtgggcttg gcattaatgt atgcaacatc atcaggaccg tattatgcgc
     1201 aaagtctttc tggttcgccg gatatcgaga tgataaaaaa tcgcatggct gaattgttta
     1261 accagtcgga tatttctgtg cgcaacaaat atagcccggc ttttgataaa tggaatgaac
     1321 tggtagataa attaaatcac gaaagaaata cagttccgtt tgcgtgtttg accaccattt
     1381 tagcgacggc gataaatgaa actcttgagg gggatacgaa tgttgccgtt gtgatggggt
     1441 gtaaatctgc gaaagatcgc acgatatcca ttgtccttgg caactcgatg ttgcaaacac
     1501 tccttgaaaa acgcctcgcc gatagtggag aaatagaaaa tctttttgat caacagggat
     1561 attttagttg cgattcatta acacccgaag aattaatgat gttaaaagac ctttttgata
     1621 ttcgagttct tcatgtctct aacaaattca atgttggatt gcaaggtaat atcaacactg
     1681 atgttttaca agattcgttt tttaaaaatg tcgatttcat tcatgaatat aactcgtata
     1741 ccgtagggat gggagtctga cgagtaaaac tcattaggat tatcagaatt cggacaatgc
     1801 attgcaaaaa tgacatacaa agagagatgt gtgcaggtac ttagccgcga ggggcatggc
     1861 gaagccatac agaaagtacc agtattcaga actccagtga aagcggcata gtggccgcct
     1921 gctcaggagt tttgctatga cccgtaatcg tgctgaacgt cgccacccta tgatgcgatt
     1981 aaaaaaactc atcgtcgcgc tgatgattga cgtcaatgct atcggatgat gccgggggtg
     2041 ttccggaagg aattccttgt ttcccgaact gttaccaaca aaattataac gatgagcaag
     2101 taaagaccgg ttggtggcag tactgaccgg tctgtgatga aagattaatt tcaccttaaa
     2161 tcctggtgct gttaggatgc tgacgttttt cggctacacc attcagatta tgtaaataaa
     2221 tatccctgaa tataatatat tcatagcacc cataactcat cctgggcgat aactaaacca
     2281 tttttgagta ataaatgaaa caactgcaac gctcttatta tttctggaaa acgcctcgca
     2341 aaaacaaaat gacatgcaaa aatccgcatt tttttaaatg cttttccggc gtaattttcg
     2401 cacatacatt aatgagtaat atatgtaagg tggtggtaat ggcgatgaat acggtttttc
     2461 ttcatttatc agaagaggca attaaacggc tgaacaagct tcggggctgg cgtaaggttt
     2521 cacgatctgc aattttacgc gaagcggttg agcaatatct ggagcggcag caatttccgg
     2581 tgcgtaaagc aaagggaggc aggcaaaagg gggaggtagt tggtgttgat gatcagtgta
     2641 aggagcataa ggaatgaaaa aaatttttca ttcttgaata taaaaaacag atgcccttat
     2701 tctggtatta atacaaggct gttttacttg aacttataat aactgcaact gttacatcgt
     2761 atctggaaaa cgcctcgcaa aaataaaaaa tgatgcgtaa atgagctttc tttaaagatt
     2821 cggcaagggt acttttctct ccatagattc aaaaatgata ttggcgagat atttatgaac
     2881 tctctggcag gtatagatat gggcagaatt ttactcgatt tatcgaatga ggtgattaag
     2941 caacttgatg atctcgaggt gcagcgtaat cttcctcgtg cggacctatt aagggaagcg
     3001 gtagatcaat acctgataaa tcaatcgcaa acagcaagaa ccagtgttcc tggcatctgg
     3061 caagggtgtg aggaagatgg tgtcgaatat cagcgtaagc tgcgcgagga atggtaatgg
     3121 gaaaaaatga tgtcaatcag attgctgaca acgtgcgcgt tgttcatgcc ggatgcggcg
     3181 tgaacgcctt atccggccta caaagtcatg caaattcaat atattgaagt ttattggtag
     3241 gcctgataag cgcagcgcat caggcaatca tacgcttgtc atcagtgaca tacccataac
     3301 cttatcgatg tgttggaggg aagaacgagg caaacagaca attgcggcat gatcttgaat
     3361 gggtaacgcg gaatacgaga gatggtgtcg tcacacccta tgagctgtaa tccgggcgac
     3421 ctgcgccacc cggaaaacgt cagagattac tctccttcac ccggaaacag gaacgggtta
     3481 atggaactac gggcgtagcc ttcttgctcc attcgcgcgt ccagtaccag agaggcgagg
     3541 tcatctgcca cggcttcaac tttcggttct ttttcctgat aaagaatctt caggtaagtg
     3601 ccgcagtcat cgcagctttc ggctttaatc gcggcctgtt cgtcatccag cgaccagtaa
     3661 tgcagtttgc cgctctgctc gcagttgctg cattttacgc gcactacgtg ccattcggtt
     3721 tcacacaggt tgcagtgcag gtaacgcaga ccctgagtgg tgccaatttg caccatgctg
     3781 gacaccggca tagagccaca taccgggcaa tattgacgtt gttcgccgta ttcagcgcgg
     3841 gctttgccgg ggatcagatt ggccatctgc gcccagtaga gcgacagcgc agcccagata
     3901 aacggcgctt tatcgctgct gacggacgag aaatcagagg caaacagcgc gctggccata
     3961 tcttccagct cctgagtcga tgccttctcc agattctcaa tcactgccag cgccgggccg
     4021 ctcatttcag gtttcagctc agcaattagc gccatcagca gcttttgcca gtgcttatca
     4081 cgcggcagaa cgtgaatatc cagcgggggc ttgccctgtg cgctggcttc tttaatgcgc
     4141 gcggtcagat ccatctccag cggatggtcg tacagcacca ctttctgggc gtgggcgata
     4201 agcgcagcaa agcgcaggta atcacccagc ggattatttt ctgccagctc gcgcagacgc
     4261 tcggcgcggc ggttgtataa attcttgagc cgagggaaca ataacggcgg aatcatatcc
     4321 gccgtacgtt tctcgctcga acccagctca tcttgcggga ttatgcgaat actcattcag
     4381 ctttcttttc cgttgtcttg cggacctcac ggtaccagcg cgggtgatgt ttcttcgccc
     4441 aggcgctggt aacccatcct tccaccatgg cggtaatcgt gcctttcacc caaagggcgg
     4501 cgtagatatg caccatgata accacaatta acgccactgc ggcaaatgaa tgcagcatta
     4561 acgcgaatcg gatcaccggg attgagaaag caggcgcaaa ataaggacgc cagataatca
     4621 cgccgctcac cagcagcaga accaggaaaa taatcgccgc ccagaaaacg catttctgac
     4681 cgaagttata acgcccggtg tcacctactt cctcgttgac gacgatctta cgaatattct
     4741 tcgcccaaaa gatatcatcc cgattgatta ggttttgatg ccagtaacgg aaaaacatga
     4801 tgatgaacga ggcaaacata accacgccga caaacgggtg cagaattcgc gccagctgcg
     4861 gtgtgcccat gatttgcatc aaccagttga aggacgggaa caaaaagccc agcccgctca
     4921 ccgccgccag gatgaagcag aaggcggtga tccagtggtt gatacgttcc agcgcggtgt
     4981 agcgcacgat ggtgtcacgt cgtttcattt gcgctcctcg tctttctctt cgtgcagatt
     5041 attctcttcc tcatccgcac ggttcggacc gacacccacg tagtggaaga tactggccgc
     5101 aaaggtagcc gcaaagccaa cagccgcgag cggtttccag atgcctttcc agaatttcac
     5161 ggtttcgctg atttccgggt tctccggcaa gccatgatac agatttggct tgtcagcatg
     5221 gtgcagcacg tacatgacgt gtgtaccacc gacgcctgcc ggatcgtaca gacccgcatt
     5281 gtcgtaaccg cgggttttca gctcagcaac gcgctcgctc gccagcgttt tcatcgactc
     5341 tttcgtgccg aagtgaatcg cgcccgttgg gcaggtcttc acgcaggctg gttcttgccc
     5401 aaccaccacg cggtcaacgc acagcgtaca tttgtagacg cggttgtctt ccgggttgag
     5461 gcgcggaatg tcgaacggac agcccgcaat gcaataaccg cagccaatgc actgctcgga
     5521 ctggaagtcg acgataccgt tggcatactg aataattgca ccttccgccg ggcacgcttt
     5581 caggcagcct ggatcggaac agtgcataca gccgtctttg cggatcagcc attccagttt
     5641 gtcgttctgc tccacttccg agaagcgcat taccgtccac gatttggcgc ttaaatcatt
     5701 ggggttgtcg tacaccccaa tgttattgcc gacggtatcg cgaatgtcgt tccactctga
     5761 acacgccacc tgacaggctt tacagccgat acaggtggta acgtcgatga gtttcgccac
     5821 ttcttcctgg aagtcccgcg cctgaggcgc gggggtcaga ccgttagtcg cggaacgacg
     5881 aatgatatct tgagattgat aagccatatg tcgtctccgt tacacctttt ccacattcac
     5941 aaggaaggac ttaaactccg gcgtctgcgt gttcgcatca ccaacgaatg gcgttaaagt
     6001 attggcaata aagccttttt tagctacgcc ttcatagccc cagtgaatcg gaataccgat
     6061 ggtatcgata tctttgccgt tcgctttcag cgtgcgaata cgtttggtca ccaccgcttt
     6121 ggctttgata tagccacggt tggaggagac tttcacggta tcgccctggg caatgccaag
     6181 tttattcgcc agcgactccc cgatttccac aaactgctct ggctgcaaaa tcgcgttcaa
     6241 cagcgcgtgt ttggtccagt agtggaagtg ctcggtcaga cgataggtgg ttccgacata
     6301 cgggaactta tcggctttac ccaatgcttc ggcgtcgtct ttaaagatac gtgcagctgg
     6361 gttcgagata acgtttggat gcagcgggtt agttcccagc ggcgtttcaa acggctcgta
     6421 gtgttccggg aacggacctt ccgccatctt atcgagggca aacagacgcc ccatgccttc
     6481 ctgctgcatg ataaacggcc cgacgccgct acccggaggc gctgcgctgt agtccggaat
     6541 atcccagccg gtccacttag tgccgtccca tttcagcaac tgacgcttcg gatcccacgg
     6601 gttaccctgc ggatctgcgg aggcgcggtt atacagaatg cggcggttaa gcggccatgc
     6661 ccatgcccag cccagcgtgt taccgaggcc agacggatcg gcgttatcac gacgcgccat
     6721 ctggttgcct tccggcgtcc agctaccggc gaaaatccag cagccacagg aggttgtacc
     6781 gtcatcgcgc aattgggcga acgagctaag ttgttggcct ttcttgacga taaccgcacc
     6841 ggttgccgga tcggtaatat cggccagcgc cttaccgttg ctctccatcg ctacttcttc
     6901 cgacttcggc tcatgcggaa tggcgtagtt ccaggtcatg ttcagcacct gatccgggtt
     6961 cgcgccaccc tgttcggcat acatcttgcg caagcgcagg aagataccgg agaggatctc
     7021 accgtcagtc agcgcaatcc ccggggcgtc cgcacctttc cagtgccact gcaaccagcg
     7081 acctgagtta acgattgaac cgttctcttc tgcgaagcag gtcgatggca gacggaacac
     7141 ttcggtctgg atcttcgacg agtcaacttc gttcagctca ccgtggttct gccagaagtt
     7201 agaggtttca gtgttcagtg ggtcgatggt gacgaggaac ttcagtttcg acagacaacc
     7261 gatcactttg tttttgttcg ggaatgaggc aacagggtta aagccctggc agatatagcc
     7321 attgaccttg ccctctttca tcatctcgaa gtattgcagg acgtcgtagc ctttatccca
     7381 cttcggcaac cagtcaaagc cccagctatt ttccgccgtc gctttatcac caaagaaggc
     7441 cttcatcata gagacgaaga atttcgggta gttgccccag tagtttacct ggccttccag
     7501 cagcggtttt ggcgtgttgg cggtaaggta ggtttgcaga tcggtctgct tctcgcttgg
     7561 cagcgtcatg taacctggca gactctgcga cagtagcccc aggtccgtca gcccctgaat
     7621 attggagtga ccgcgcaggg cgttaacgcc gccgccggcc atccccatat tgccgagcag
     7681 cagctggatc atcgccatcg tacgaatgtt ttgcgcacca acggagtgtt gcgtccagcc
     7741 gagggcatac aggaacgagg cagttttatc gtgagcactg gtttctgcga tgtattcgca
     7801 gactttcagg aacgcgtctt ttggcgtacc acagatgttt tcaaccacat ctggcgtgta
     7861 gcgggaaacg tgctgtttca gcaagttcca cacgcagcgc ggatgttgca gcgtggtatc
     7921 gcgtttggcg aagccgtttt cgtccagttc ataagtccag gaggatttat cgtacttgcg
     7981 tttttccgcg tcgtagccgg tgaacaggcc atcttcaaag ccgtaatcct cacgcacgat
     8041 caggctggcg ttggtatagg cttcggtgta ttcgtggttg aatttttcat tgttcagcag
     8101 gtacagcaat acgcctgaca ggaaagcaat gtcagtaccg gaacgaatag gggcatagta
     8161 gtcagctacc gccgccgtac gcgtaaagcg aggatcgatc acaatcagct tcgcgccgtt
     8221 gtgaattttg gcttccatcg cccagcggaa cccgaccggg tgagcttcag cggcgttacc
     8281 gcccatcacc acgacgaggt tggcgttctt gatgtcgacc cagtggttgg tcatcgcacc
     8341 gcgaccaaat gttggagcaa gacttgctac cgttggtccg tgtcagacac gcgcctggtt
     8401 gtcgaccgcg agcataccca gcgcgcggga gaatttttgc gttaaatagc cggtttcgtt
     8461 actcgacgcg gaagcacaca gcatcccggt ggagagccag cggttaacag tcacgccttc
     8521 ggcgttttgc gcaatgtagt tagcatcgcg gtcttctttc atcagtttgg cgatgcgatc
     8581 aaacgcctct tcccaactga tttgctgcca tttatcagaa cctggcgcac ggtattccgg
     8641 aaacttcaga cggctttcgg agtggatgaa atccaccagg ccagcgcctt tcggacaaag
     8701 tgcaccgcgg ttgaccgggt gatccggatc gccttcgata tggaagatag atgctttggc
     8761 gttttttgct ccgtcaccga ggctgtacat caacagccca cagcctacgg aacaataggt
     8821 gcaggtatta cgggtttcgc gggtgcgcag cagtttatac tgccgggttt ccgcgagtgc
     8881 tacgctgggt gcaaaaccca gtgccgctgc cgtggtgcct gccataccgc cagcgcagat
     8941 cttaaagaac tgccttctgc tgacctgcat ggattgctcc ttgtttcgac attgtcacgt
     9001 cccatttaca ttcgcttgct gcgtgtgcag ggagtgggag ttatttttct ttgcggaagg
     9061 ggccgcaaag gtccagaatt ggctcaattt ccctccatct aggaagggtt tgtaacagaa
     9121 taccataatg ttggtgtgta tgttcttatc tggttaagag aaagtgaaaa aaacacagcg
     9181 aaaagaaatc gaaaatgtga caaatatcac aggtgttcgt caaattgagt tatggcggcg
     9241 tgatgattta caacacccac ggttagatga ggtcgcggaa gaagttcccg ttgcgctggt
     9301 ctacaacggc atttcgcatg tggtgatgat ggcgtcgccc aaagaccttg agtactttgc
     9361 gctcggtttt tcgctttccg aggggataat agaaagtccg cgcgatatct tcggcatgga
     9421 tgtcgttcct tcctgtaatg gtcttgaagt acaaattgag ctttccagcc gccgctttat
     9481 ggggttgaag gagcgccgcc gggcgctggc gggacgtacg ggctgtggcg tatgcggcgt
     9541 ggagcaactt aatgacatcg gaaaaccggt gcagccgcta ccgttcaccc agacgtttga
     9601 tctcaacaaa ctggatgatg cattacgtca tctcaacgat tttcagccag tggggcgact
     9661 gactggttgt actcacgccg ctgcctggat gttgccatct ggcgaactgg tcggcgggca
     9721 tgaagacgtg ggtcgccatg tggcgctgga caaactgtta ggccgccggt cacaagaagg
     9781 ggaaagctgg cagcaaggtg cggtactggt ttccagtcgt gccagttatg aaatggtgca
     9841 aaagtcggcg atgtgcggcg tagagatttt gtttgcggtg tctgccgcga ccacgcttgc
     9901 tgtagaagtg gctgagcgct gtaatctgac actggtaggt ttttgtaaac cgggtcgggc
     9961 aacggtttat acccatccgc agcgtttgag caattaatta aaataagtcc tgcgatatat
    10021 attaaattgc aggacttatt catttc
//
LOCUS       DAETQL010000125        10028 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.125, whole genome shotgun sequence.
ACCESSION   DAETQL010000125 DAETQL010000000
VERSION     DAETQL010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.125"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(13..1227)
                     /gene="rspA"
                     /locus_tag="K0W65_003609"
     CDS             complement(13..1227)
                     /gene="rspA"
                     /locus_tag="K0W65_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HBI3096715.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1433..1759)
                     /locus_tag="K0W65_003610"
     CDS             complement(1433..1759)
                     /locus_tag="K0W65_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HBI3096716.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1894..2235
                     /locus_tag="K0W65_003611"
     CDS             1894..2235
                     /locus_tag="K0W65_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HBI3096717.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2301..2830
                     /gene="speG"
                     /locus_tag="K0W65_003612"
                     /pseudo
     CDS             2301..2830
                     /gene="speG"
                     /locus_tag="K0W65_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2833..3543)
                     /locus_tag="K0W65_003613"
     CDS             complement(2833..3543)
                     /locus_tag="K0W65_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HBI3096718.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3651..3956
                     /locus_tag="K0W65_003614"
     CDS             3651..3956
                     /locus_tag="K0W65_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HBI3096719.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4155..4475
                     /locus_tag="K0W65_003615"
     CDS             4155..4475
                     /locus_tag="K0W65_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HBI3096720.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            5035..5613
                     /locus_tag="K0W65_003616"
                     /pseudo
     CDS             5035..5613
                     /locus_tag="K0W65_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6295..7284)
                     /locus_tag="K0W65_003617"
     CDS             complement(6295..7284)
                     /locus_tag="K0W65_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096721.1"
                     /translation="MTMIKDSYQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLSDRSVVVPVRGGERPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTHGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTTKADAIMDALFENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTVKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7361..7852)
                     /locus_tag="K0W65_003618"
     CDS             complement(7361..7852)
                     /locus_tag="K0W65_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096722.1"
                     /translation="MLFNNNDWKLSVTDINLYENTVSLDGQSYPLSFAIKTLIPGYLS
                     GLPATSRESMELLEALAEAGVAIGNFFSNDLMTAYQRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQRELQRREQVKAERRTYGENLHSATHSAGRSRAAIVADIESGGNWM
                     DSL"
     gene            complement(8171..8668)
                     /locus_tag="K0W65_003619"
     CDS             complement(8171..8668)
                     /locus_tag="K0W65_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096723.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDTE
                     STQYKRFAQTKVGKLLRNRQKIRLIRMGELGSTVLADPAATVEFGRWLDFEYGLAVSE
                     AFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTAGR
                     CEWGK"
     gene            complement(8658..9173)
                     /locus_tag="K0W65_003620"
     CDS             complement(8658..9173)
                     /locus_tag="K0W65_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096724.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDPEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDTERLQAKADQEQRFKSFA
                     EALAGGASYDF"
BASE COUNT         2561 a         2536 c         2506 g         2425 t
ORIGIN      
        1 ttatcttact ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata
       61 agcaggttca tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg
      121 tttgtctccc ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag
      181 catttgttcg gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa
      241 gtgcagcgcc gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg
      301 tacctgatac agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg
      361 ggtcagcgtg gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca
      421 gatgctgttg aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg
      481 gaagcattct tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc
      541 aatgctttta ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag
      601 caaatgttca ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc
      661 gaggtatttc tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc
      721 ataagccaga cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca
      781 ctgcacgcgg atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc
      841 atcaatactg tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc
      901 gagtaactgg taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc
      961 aaccgctgaa atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa
     1021 aaactgccag atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg
     1081 atcctgcaaa taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc
     1141 cgtaataccg tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac
     1201 aaaaacttca gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat
     1261 gaaatctacg caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga
     1321 tcacaaaaaa aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc
     1381 ttttgtcagc tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc
     1441 gcccccagcc cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt
     1501 cataaagact tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat
     1561 aaacgccacc ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc
     1621 agacaaacag cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt
     1681 ttaaccacaa ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata
     1741 gtaacgttgt tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca
     1801 taatatcatc aatataaaca tcctcctggt acattttgcg ttcgcagtgg atggtgtaga
     1861 atcaccttta atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc
     1921 ggcctgctcg cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa
     1981 actaacaaac tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg
     2041 gaaaaagagc aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa
     2101 aaagagtggg ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc
     2161 aacatcaatg cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc
     2221 gttattaccc cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc
     2281 ccacagttaa gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca
     2341 ataacgccag tgtgatgcgt tactggtttg aggaaccctg cgaagccttt gttgaactct
     2401 ctgatctgta tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg
     2461 tgacggcgaa aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc
     2521 agaatttcag ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc
     2581 caaattagca atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt
     2641 cgataaagag aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg
     2701 tgaattgatg catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat
     2761 attccagcat cagtatctgg cagagcacaa aacaccggat cagactctcc tgaagccgac
     2821 cgcacaatag cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc
     2881 gggttttact ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc
     2941 atattcacag ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt
     3001 gtaatctaat tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc
     3061 gttacttttc gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg
     3121 catttgtttg ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc
     3181 ggcagaaggt aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt
     3241 ctcggcatca cggtaataat tggcgtccag taccagagcg accacggtat tattttccag
     3301 atccagtaat tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt
     3361 cgtcacttca ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag
     3421 cggatcaaaa tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac
     3481 ctctgtgcgg tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt
     3541 cacaacagtc accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta
     3601 cacgttaaat gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct
     3661 caacctgctg tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg
     3721 gatcttgtga gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg
     3781 aaagcagctt cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg
     3841 tcgtcgggca ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta
     3901 acgtctctgc tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca
     3961 ttatcgactg aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac
     4021 cttattactc atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga
     4081 ctccctctat tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga
     4141 acagggagtg agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg
     4201 ttaaatcaac cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca
     4261 ccctgcgcag tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct
     4321 gttccgtcaa ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga
     4381 cctgggtgga aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc
     4441 tgtttgcgcg gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca
     4501 atgaaacgcg tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc
     4561 ctggatacta tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta
     4621 tatattcagt actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc
     4681 tcggcggtca aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac
     4741 agcaccgcgc aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc
     4801 accacggata ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc
     4861 cgcatgagcg gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc
     4921 aaaatgattg ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg
     4981 ctccctattc gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt
     5041 aacgaaaatc tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa
     5101 accttacccg cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt
     5161 gacgataaaa cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac
     5221 cgtatcatca aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc
     5281 tgggggccgc aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct
     5341 attttgacgg gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat
     5401 accattacga tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc
     5461 ttcagctgga cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc
     5521 cggggcaaag ataaactgga tgtgcccatt aagttcatct ggaacttcaa ggacttgaag
     5581 cggaccctta gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc
     5641 cagacttgtg tcattagacg ggatgtctag acggctggtc aggacccccg gtggcctcgg
     5701 tctggacttg aggtgggcat atgggggaag tctgctggct taatgtatta gatggtcatt
     5761 cacatttttc tgtcagaaac catcggtccc actgtgagtc caaacctaag gacctagaag
     5821 tccacccaca gccttagaca gagtgtcgaa cacatccaca ccgagtcgac cagagaggac
     5881 catgtagaga cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag
     5941 gtctgcggaa gtcatggaca taaagcctcc ttcgtctatt gtactactgc actagataac
     6001 tagtacaaaa caaaggtcca gaccattggt ccagaccata gttagttaat cacagtgata
     6061 gcccaccacc tgaagtcctg ctgatattca gtccagactt acctctgctc atggtcttac
     6121 ctccctcggt ctcctgacct tgggtatcga ctacagcttg ctgagcgtca tctggaggag
     6181 ctacaggagg agctacagga ggcgggaccg aagccccacc ttgagtccca ccaccacctg
     6241 tcaggccctt ggcgatggac ctgaagcctt tactaatgct cttacccatt ggtcttacgc
     6301 ttcggtcttg gtgaacttca ggcgtttgac tacaggcagg acggcagtag ctgagccagt
     6361 gcccatcttg acggtcgtag cagcttcagc gacaacgaac actttaccga ggatttccaa
     6421 gtcgtgggac tgagagtcgc catcacgtga aaggacgacc gtaatattct caccgtcgtg
     6481 acggaagcag ccgaagctca gggccgtgaa gcgtttagcc agcatgaaca cacctttgtc
     6541 ctcaccgttg taatacgcga tgtcggtacc cagcatatcc acgatagcgt cttcaccaga
     6601 gaagccctgt ttagcagcca gacgggacag cgctttcagt tgggactcat gggcgatgat
     6661 gccgtaatca gacacttcag agccgaaaga ggactcggtg ttctcaaaca gagcgtccat
     6721 gatagcgtct gccttagtgg tcaggcttcc agtcaggact ggcagctcta cggtcttcag
     6781 cagcttggag gactccagaa cgtcaaccat gcgagcgttg atggtacgga ccacattctc
     6841 acagatgttc atagcgtcct gctggagtgt gccatgagtg aaaccagtgg aggacttggc
     6901 gtcccacagt ttagtttgct ccgagatgag gattgcctgt tcgataggtt cgacgacaat
     6961 ctcctgacca gacggaacgg tcacgacttc aggtcgctca ccaccgcgta cagggacgac
     7021 cacagagcgg tcagacagag tgtgggcgat gattggggta ggcaggctat tgcgggtagg
     7081 caggacttct acagggaacg gaatgttatc ctgagcgtct ttaagtacac cgttcagcat
     7141 agccttaacc gtcttagcta actggagggc atagacctgt gggtcagcgt tagttggcag
     7201 accttcgtta acctgaatgt tgtcggtgtc ggtgatacgt actggctcaa tgccgaactg
     7261 atagctatct ttaatcatgg tcataataat caatctccta aattagtttt agatactgtg
     7321 ggtcccgaca tcaggtcagg accacttgtg tgtctgtgtc ttagaggctg tccatccagt
     7381 taccgcctga ctcaatgtca gcgacaatag cggcacggga gcgaccagcg gaatgggtag
     7441 cactgtggag gttctcacca taggtccggc gctcagcttt gacctgttcg cggcgctgta
     7501 gctcccgctg ccactctgcg tcggtcatgt tctcttcacg catacgctcg gcctgaagtc
     7561 gttgctcttt agcgatacgt tcagcctcag ctcgtttgtt catctggcgg cgttggtagg
     7621 cggtcatcag gtcattactg aagaagttgc caatagccac accagcttca gccagagctt
     7681 ccagtaattc catggactcg cggctggtgg caggcagtcc tgacaggtag cccggaatca
     7741 gggtcttaat agcgaaggac agtgggtagg actgaccgtc cagactcaca gtgttttcgt
     7801 agagattgat gtcggtgact gacagtttcc agtcgttgtt gttgaataac ataaattggt
     7861 actcctcttg agagttgaga ctatcggtat cgcagacgca gtagcgccta cgggtctccc
     7921 cgcatcggtc atctcaaaag gtgtttaaat taggtatggt atggtcagga ctgcttagca
     7981 aacgctcggc gtaactgatt attcaaatca cggtccacca gcatggctat cagctcgtct
     8041 cgactgtaga aaggacgctc ggggtcttcg actccaagtc tcagcagaga caggaggtga
     8101 cgtgtaggca gaccacggta acgaagggtc gctttgccga taggtgtgcg agggtcaatc
     8161 atagccatga ttacttaccc cactcacagc gaccagcggt cacagcttga tagtctgaat
     8221 actcagcctg tgagacttca cgtccggcga tgtagaggcc tgtgccatga cgctggatga
     8281 attccttggt ctccggcttg atgtcctcag cagacgctac agcagactgc acggtggcgc
     8341 tggaggtcat agacacgaag gcttcagaga ctgccagtcc gtactcaaag tccagccagc
     8401 gaccaaactc tacagtagcg gcggggtcag caaggacggt agacccgagt tcacccattc
     8461 ggataaggcg gattttctgc ctattcctca gtagctttcc aactttggtc tgagcaaaac
     8521 gcttgtactg tgtcgactcg gtgtcttcta ctttgccaat ctgaatgccg acccggcgaa
     8581 tttcatcgag tcgaaacaat ccgtcctgac tggtgatgtc ctgaccgaag atatgaatgg
     8641 tgtattcctg cttattgtca gaagtcataa gaggcacctc ctgccagagc ttcggcaaag
     8701 gacttgaagc gctgctcttg gtcagctttc gcctgaagac gttcggtatc aggtgggacc
     8761 agcttatcaa aggtgccgtt ttcgtaatcc tcagttagga cctgaagtcg gcgggaccat
     8821 ttgctctcca cctgcatcat cgcgttagag atgtgcttca agtcggcatc agagcgtgac
     8881 gctatccaat ccatctcttc tgggtcaccc tcacaggtgt aaggattgtt gaaatagaag
     8941 atgtcctgta ggccacgttt cagctcttcc tcggtgtgtg tctggaggta tgactggata
     9001 ggctccgcgt acatctcccg gattttctca tagtcccagt cttccgcttg ggcataccat
     9061 cgggataact tcagcaggtc taagttatag cgacagcaat cgacactgga ctctgcattg
     9121 gtcagcagga tgattaagaa ctggcggtta gttactggta attcggtatt catataaagg
     9181 atgctccttt ggtaaagcac tttctgtctc cacgtggagt ccgcacctat tcaatgcatc
     9241 caaacggacc caccaaaaag tgcttaacga aagtgaatag gattaaggat ggtctggagt
     9301 gacccggtgt ctctacatgt acgtataaag gcgagaccgc atgtcaggac gagctgtaag
     9361 gaccccagat ggtgagcggt gatagactta agccaatgta gtgagtagac gagctactaa
     9421 ctgagatgac tgaagaacgc taacgagctg gaggtcatac aggaggtctg acagaggcgg
     9481 tcacagacct tgactactta ggagagatac ttaatgtcgg ttgaccagat gctcgcatgt
     9541 tctgacgcat cagactcaga ggcaaggttg gtctccctat agtgatagtt taggggggtg
     9601 tccctatagt gagggggtat tccagcccag agctggcgcg gctctcagga caggcactta
     9661 tcgttgtagt cccaccatgt gaaatcccct cggtccttca gaaccttagc aagactcaca
     9721 gccgtagcct gcaagacgct attaacgtgg cgcaggtagc gaggcgaagg tgagcgaccc
     9781 gtgatggcgg tgtatgactt ggcagtgttg cgggagaaga tgtcgtttgt gccgtataag
     9841 gccgcgaaca ggtacgcctt ggataccggg agtccagcac cgatagagcc actggtagcc
     9901 cgtgagagtc ctgtgaacac atcgtcagcc cacgggtacg cctcaacaag ctgcgtatag
     9961 gagagacacc cagtctcaat cagccacgca cggacagccc ggttgtcgat gaccacagga
    10021 ggcttacc
//
LOCUS       DAETQL010000126        10022 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.126, whole genome shotgun sequence.
ACCESSION   DAETQL010000126 DAETQL010000000
VERSION     DAETQL010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.126"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..116)
                     /locus_tag="K0W65_003621"
     CDS             complement(<1..116)
                     /locus_tag="K0W65_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3096725.1"
                     /translation="MSKPKYPFEKRLEVVNHYFTTDDGYRIISARFGVPRTQV"
     gene            548..1666
                     /locus_tag="K0W65_003622"
     CDS             548..1666
                     /locus_tag="K0W65_003622"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBI3096726.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FMALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1732..1980
                     /locus_tag="K0W65_003623"
     CDS             1732..1980
                     /locus_tag="K0W65_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HBI3096727.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            2045..2413
                     /locus_tag="K0W65_003624"
     CDS             2045..2413
                     /locus_tag="K0W65_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBI3096728.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2507..3160
                     /gene="nfsB"
                     /locus_tag="K0W65_003625"
     CDS             2507..3160
                     /gene="nfsB"
                     /locus_tag="K0W65_003625"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBI3096729.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3268..4515
                     /gene="ybdG"
                     /locus_tag="K0W65_003626"
     CDS             3268..4515
                     /gene="ybdG"
                     /locus_tag="K0W65_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HBI3096730.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4583..5959)
                     /gene="pheP"
                     /locus_tag="K0W65_003627"
     CDS             complement(4583..5959)
                     /gene="pheP"
                     /locus_tag="K0W65_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBI3096731.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(6060..9203)
                     /gene="cusA"
                     /locus_tag="K0W65_003628"
     CDS             complement(6060..9203)
                     /gene="cusA"
                     /locus_tag="K0W65_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HBI3096732.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9215..9733)
                     /gene="cusB"
                     /locus_tag="K0W65_003629"
                     /pseudo
     CDS             complement(9215..9733)
                     /gene="cusB"
                     /locus_tag="K0W65_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2461 a         2759 c         2429 g         2373 t
ORIGIN      
        1 acctgggttc gggggacacc aaaacgtgcc gagatgatcc tgtaaccatc atcagtagtg
       61 aagtagtgat tcacgacttc aaggcgcttt tcaaaagggt attttggctt tgacatatta
      121 ggggctattc catttcatcg tccaacaaaa tgggtgcagt acagggttga tagaaatatc
      181 tcctggggct tcatctttcc agacctcaga gtaaacctga aatcggaaag cttcaggcac
      241 ccgcagttat cttacccttc ctcaacaaaa tagctgttta tttatacagc aaatcctgtt
      301 tcggacttct cactccccat ttgatcaact gaagctgaac ccgttttttt ctgcatgcct
      361 caatcgtcaa aattcttcgc caccagccag ctctgccata acttgccaca ttgaaaaagt
      421 agccaaagtg cttcaggcac catcagttca ccatcaccaa aaaaaagaaa cattccagaa
      481 cttccgcatc tcgcccagcc gctccgactt ctatactgaa tagaaaacgt caacttaaga
      541 gaaacctatg ccattacccg attttcatgt ttctgaacct tttaccctcg gtattgaact
      601 ggaaatgcag gtggttaatc cgccgggcta tgacttaagc caggactctt caatgctgat
      661 tgacgcggtt aaaaataaga tcacggccgg agaggtaaag cacgatatca ccgaaagtat
      721 gctggagctg gcgacggatg tttgccgtga tatcaaccag gctgccgggc agttttcagc
      781 gatgcagaaa gtcgtattgc aggcagccgc agaccatcat ctggaaattt gcggcggtgg
      841 cacgcccccg tttcagaaat ggcagcgtca ggaggtatgc gacaacgaac gctatcaacg
      901 aacgctggaa aactttggct atctcatcca gcaggcgacc gtttttggtc agcatgtcca
      961 tgttggctgt gccagtggcg atgacgccat ttatttgctg cacggcttgt cacggtttgt
     1021 gccgcacttt atggcccttt ccgccgcgtc gccatatatg cagggaacgg atacgcgttt
     1081 tgccccctca cgaccgaata ttttttccgc ctttcctgat aatggcccga tgccgtgggt
     1141 cagtaactgg caacaatttg aagccctgtt tcgctgtctg agttacacca cgatgatcga
     1201 cagcattaaa gatctgcact gggatattcg ccccagtcct cattttggca cggtggaggt
     1261 tcgggtgatg gataccccgt taacccttag ccacgcggta aatatggcgg gattaattca
     1321 ggccaccgcc cactggttac tgacggaacg cccgttcaaa catcaggaga aagattacct
     1381 gctgtataaa ttcaaccgtt tccaggcctg tcgctatggt ctggaaggcg tcattaccga
     1441 tccgcacact ggcgatcgtc gaccgctaac ggaagatacc ttgcgattgc tggaaaaaat
     1501 cgccccttca gcacataaaa ttggcgcatc gagcgcaatt gaagcactgc atcgccaggt
     1561 cgtcagcggt ctgaatgaag cgcagctgat gcgcgatttc gtcgccgatg gcggctcgct
     1621 gattgggctg gtgaaaaagc attgtgagat ctgggccggt gactaagccg ggaattgcct
     1681 tacagaagaa tatccctgac aatggtgttt taacttacat ttaacaatgt tatgaaacac
     1741 cctttagaaa ccttgaccac cgcagcaggc attttgctga tggctttcct ctcttgcctg
     1801 ctgctgcccg cccccgcact ggggctgacg ctggcacaaa aactggtgac catgttccat
     1861 ctgatggatc ttagccagct ttacacttta ttgttttgtc tgtggttttt agtgctgggc
     1921 gctattgagt attttgttct gcgctttatc tggcgacgct ggttctcgct ggcggattaa
     1981 acgtcgatta tcgccgaact tcgcggcata ctttgcttat tctccttcgc ctaaaggaac
     2041 gtttatggat aagcaatcac tgcacgaaac ggcgaaacgc ctagctcttg agttaccctt
     2101 tgtcgagctt tgctggcctt ttggcccgga gttcgatgtt tttaaaattg gcggcaagat
     2161 ttttatgctg tcgtcggagc tacgcggcgt cccctttatc aatctgaagt ccgatccaca
     2221 aaaatccctg ttaaatcagc aaatataccc aagcattaag ccagggtatc acatgaataa
     2281 aaagcactgg atttcggtgt attccggcga ggaaatctcc gaagcgttac ttcgcgatct
     2341 gatcaacgat tcgtggaatc tggtggttga tggtctggct aaacgcgatc aaaaaagagt
     2401 gcgtccaggc taaagcggga atctatagcg catttttctc gcttaccatt tctcgttgaa
     2461 ccttgtaatc tgctggcacg caaaattact tacacatgga gtctttatgg atatcatttc
     2521 tgtcgcctta aagcgtcatt ccactaaggc atttgatgcc agcaaaaaac ttaccccgga
     2581 acaggccgag cagatcaaaa cgctactgca atacagccca tccagcacca actcccagcc
     2641 gtggcatttt attgttgcca gcacggaaga aggtaaagcg cgtgttgcca aatccgctgc
     2701 cggtaattat gtgttcaaca aacgtaaaat acttgatgcc tcgcacgtcg tggtgttctg
     2761 tgcaaaaacc gcgatggacg atgcctggct gaagctggtt gttgaccagg aagatgccga
     2821 tggccgcttt gccacgccgg aagcgaaagc cgcgaacgat aaaggtcgca agttcttcgc
     2881 ctatatgcac cgtaaagatc tgcatgatga tgcagagtgg atggcaaaac aggtttatct
     2941 caacgtcggt aacttcctgc tcggcgtggc ggcgctgggt ctggacgcgg tgcccatcga
     3001 aggttttgac gccgcaatcc tcgatgcaga atttggtctg aaagagaaag gctacaccag
     3061 tctggtggtt gttccggtag gtcatcacag cgttgaagat tttaacgcta cgctgccgaa
     3121 atctcgtctg ccgcaaaaca ccaccttaac cgaagtgtaa ttctctcttg ccgggcatct
     3181 gcccggctat ttcccctcag attctcctga tttgcataac cctgtttcag ccgtcatcat
     3241 aggctgctgt tgtataaagg agacgttatg caggatttaa tatcccaggt tgaagattta
     3301 gcgggtattg agatcgatca caccacctcg atggtgatga ttttcggtat tatttttctg
     3361 accgccgtcg tggtgcatat tattttgcat tgggtggtac tgcggacctt cgaaaaacgt
     3421 gccatcgcca gttcacggct ttggttgcaa atcattaccc agaataaact cttccaccgt
     3481 ttagctttta ccctgcaggg gattatcgtc aatattcagg cggtattctg gctgcaaaaa
     3541 ggcaccgaag cggcagatat tctgactacc tgcgcgcagt tgtggatcat gatgtatgcg
     3601 ctgctttcag tcttctcgtt gctggatgtt attttgaatc tggcgcagaa attcccggcg
     3661 gcatctcagt taccgctgaa agggatattt caggggatta aactgatcgg cgcaatcctg
     3721 gtcggcattt tgatgatctc gctgctgatt ggtcagtcgc cagcgattct aatcagcggt
     3781 cttggtgcaa tggctgccgt gctgatgttg gtatttaaag atccgattct tggtctggtg
     3841 gcaggtattc agctttccgc gaacgatatg ctgaaactgg gcgactggct ggaaatgccg
     3901 aaatacggcg cggatggcgc ggtgatcgat attgggttaa ccaccgtcaa agtgcgtaac
     3961 tgggacaata ccattaccac tattcccacc tggtctctgg tttctgactc ctttaaaaac
     4021 tggagcggga tgtcagcatc tggcgggcga cgtattaagc gcagtatcag tattgatgtc
     4081 accagtattc gttttcttga tgaagacgaa atgcaacgtc tgaataaagc gcatttgtta
     4141 aagccttatt taaccagccg ccatcaggaa attaatgagt ggaatcgcca gcaaggttct
     4201 acggagtcgg tattaaacct gcgccgaatg accaatattg gaacctttcg tgcctatctg
     4261 aacgaatatc tgcgtaacca tccgcggatt cgtaaagata tgaccttaat ggtacgccaa
     4321 ctggctccag gtgataacgg tttaccgctc gagatctacg cgtttactaa caccgtggtg
     4381 tggctggaat atgaaagcat tcaggctgat atattcgatc acatatttgc gattgtcgaa
     4441 gagtttggtc tgcgacttca tcagtcgcca accggcaatg atattcgctc tctggcgggt
     4501 gcatttaagc agtaattaaa aaaaccgctc tcatcgaatg gatgagagcg gacatcgttt
     4561 tgtcggatgt agcgtgaatg ccttatttcc gacgcagcgt tttaaatgcc acaaacagga
     4621 atacaatcca caccggcagc aggatcgctg acaagcgcat atcatccatc gtgcacatca
     4681 gcagcaaaat catgccgagg aaggcgatgc aaagatagtt gccgaacgga tagagcagcg
     4741 ccttaaactg tgtttcacgc ccctgacgtc gcatcgccgc gcgaaaacgc agatgcgcca
     4801 ggcagatcat aatccagttc aacagcaacg ttgctaccac cagcgccatc agcagaccaa
     4861 acgctttttg cggcagcaga tagttgatta acaccaccag cgaagtgatc gctccggaaa
     4921 gcatcagcga gttaatcggc acaccgcgac ggctgacgcg agtcaaaaac ttcggcgcat
     4981 taccctgcac agaaaggcca aacagcatgc ggctgttaga gtaaaccccg ctgttataca
     5041 ccgacagcga tgccactaga atgacgaagt tcagcgcaga agctaccaca ttgctgtcga
     5101 gattatggaa aatcatcaca aacgggctac tgttggattt cacttccacc cacggataga
     5161 gtgccagtaa aaccaccagt gaaccgatat aaaacagcag gatgcgatac accacctgat
     5221 ttaccgcttt tggaatgctt ttttccggat cgcgcgcttc agcggctgta atcccaatca
     5281 gctccagacc gccgaaggag aacataatta ccgccagcga caaaatcagc ccattccagc
     5341 cggtggcgaa gaaaccaccg tagcgccaga ggttgtcgat actggctttc tcgccgccgt
     5401 aaccagaaaa cagcagccac aggccaaagc cgatcatacc gatgatcgcc agcactttaa
     5461 tcagcgcaaa ccagaactcg gtttcgccat ataagcgcac gttcaccagg ttaacggcgt
     5521 tgatgataat aaagaaggcg gcagcccaaa tccacgttgg aacatccggg aaccagtact
     5581 gcatatagat gcccgcagcg gtcagctctg ccattcccac cagcacgaac attacccagt
     5641 agttccagcc agagaggaag cccgcaaacg gtccccagta tttataggca aagtgggcaa
     5701 atgaaccgga taccggctct tcgaccacca tctcgccaag ctggcgcata atcaggaaag
     5761 cgatgatccc ggcgacgccg tagcccagca atacagccgg acccgccatc tgaatcgccg
     5821 ggccaatgcc aagaaacaga ccagtaccaa ttgcgccacc cagcgcaatc agttgaatat
     5881 gacggttatg taatccgcga tgaagcgtcg gctcttgatt cgacgcagta tcttccgata
     5941 cggttgacgc gtttttcacg cctttcccct gtgtgtcttt tttgttgagg ggcacctttt
     6001 aacatttagt gcccatcgtc gcaagacaca atccacacgg ttaaacgagg tatcctgctt
     6061 taattccgta cccgatgtcg gtgcagccac atcagcttat acgccgccgg gataataaac
     6121 agcgacagca aaggtgcggt gatcatgccg ccaatcatcg gtgcggcaat ccggctcatc
     6181 acctctgaac cagcccccgt tccccacaga atcggcagca gaccggcgat aatcaccgcc
     6241 accgtcatcg ctttcgggcg cacgcgcagg accgcgccgt gatacaacgc ctcatccagc
     6301 ttctgctcgc tgaatgtttg tggattattc aacgacggct cggcctctat ggcgtgacgt
     6361 aaatacatca gcatcaccac gccaaattcg gcggcgaccc cggcaagggc aataaagccg
     6421 gtacccgtcg ccacagaaag atgaaagccc atccaccaca gtaaccagat gccgcccacc
     6481 agcgcaaacg gtacgctgct gataatcagc aacgcttcgc ccacccgacg gaatgccaga
     6541 tacaacagca cgaagatgat catcagtgtc atcggcacca tgagtttcag cttatggttg
     6601 gcgcgctcca gtagctcgaa ctgcccggag aatgccacgc tggtgcccgg ttttaactgc
     6661 actttttcag ctatcgcttt ttgcaaatcg tgaaccaccg acaccatgtc acgatcgcgg
     6721 gcatcgatat aaatccagct cgtcgggcgc gcattctcgg ttttcagcat cgacggtccg
     6781 gtagagactt taacgtcggc cacgtctgcc agggtgattt gctgcttcat cggcgtcagg
     6841 atcggcaact ggcgcagtgc ctgcggacta tcgcgccagc tttgcggata acgcagatta
     6901 attggataac gggcaatccc ttccaccgtt tcgccaacca tcgccccgcc caccgcagaa
     6961 gtcacaaaca actgcacatc cgccaccgtc ataccgtaac gtgcggcttt ttcacggtta
     7021 atctcaacgt tgatatagcg cccaccttcc agccgctcag caagcgcaga agctacgcct
     7081 ggcaccgttc gcgccacttc ttcaatttgc tcagccatcg cgtcgatatc cgccagcaca
     7141 gtgccggaaa ctttaatgcc gatggggctt ttaatgccgg ttgagagcat atcgatacgg
     7201 ttacgaattg gcggcaccca cagattcgcc agccccggca gacgcacggt gttatccagt
     7261 tcctcaatga ttttgtccat tgtcatgcct ggccgccact gatcctgtgg cttaagctgg
     7321 atggtcgttt ctaccatctc cagcggagca gaatcggtgg cggtttccgc tttcccggtt
     7381 ttgccaaata cccgcgccac ttcaggtacg ctcataatta gcttgtcggt tttttgcagc
     7441 atactcgccg cctctgctgc ggaaatcccc ggcagcgtcg atggcatata caacaagtcg
     7501 ccttcattga tctgcggtaa aaattccccg ccaactttat tgagcggcca gagaaccgtc
     7561 agcaccgaaa gcgccgccac cagcagcgtg gttttcggcc agtgcagtac tttcagcaac
     7621 agcggatgat aaacacgaat caaaaagcga ttgagcggat tactgctttc cggcggaatt
     7681 ttgccacgga tccagtaacc catcaggata gggatcacta cgatcgccag cagcgccgca
     7741 cccgccatcg catacgtttt ggtgaacgcc aacgggccaa acagacgccc ttcctgccct
     7801 tccagggtga agatcgggat aaacgacaac gtgataatca gcagactgat aaacagcgcc
     7861 ggccccactt caacagacgc atcggtgatc acctgccagc gcgttttatt atccagcgtg
     7921 gcgtcaggat gctggtgctg ccactcttcc agccgtttat gcgcattctc gatcatgacg
     7981 atagcggcat cgaccatcgc cccgacggca atcgcaatgc cacccagcga cataatattg
     8041 gcattcagcc cctggaaggg catgacaata aaagcaatac acaaccccag cggcaacgaa
     8101 ataatcgcca ccagcgccga gcgcacatgc cagagaaacg gcgcgcagac taccgccacc
     8161 acaataaact cttccagcaa cttgccgctg aggttgtcga tggcccggtc aatgagctgg
     8221 ctgcggtcgt atgttgtaac tatctccacg ccttccggca gactactttt cagcgtttcc
     8281 agtttgtcct tcacggcggc gatcacttct cgggcgtttt tgccggatcg caggatcacc
     8341 accccgcccg ccacttcgcc ttcgccgttt agttcggcaa tgccccggcg catcttcggg
     8401 ccaatctgga ccttcgcaac atcgcgcaga taaacgggca cgccattttc actggctttt
     8461 aaaacgatgt gattaaagtc gtcgagcgtt tgtagatagc cgctggcgcg caccatatat
     8521 tctgcttccg ccagttcgat tgacgaaccg cccgcttcct ggtttgaagc atccagcgcg
     8581 ctttttactt cggcgagact gatgccatac tgcgccaggc gctgaggatc gataaccacc
     8641 tgatactctt tcaccacacc gcctaccgac gccacttccg caacgtcagg aatggttttc
     8701 agctcatatt tgagaaacca gtcctgcaat gagcgtaaat cggcaagatc gtgcttaccg
     8761 ctgcgatcca ccagtgcata ttcatagatc cagccaacac ccgtggcatc tggtcccagc
     8821 tcggcgctga ctcccgcagg cagcttaccc tgtacctggt tgaggtactc cagcacccgc
     8881 gaacgcgccc agtacggatc ggtgccatct tcgaaaatga catacacata agagtcgcca
     8941 aactgcgaga aaccacgcac agtcttcgcg ccaggcaccg acaacatggt ggtggttagc
     9001 ggataagtca cctgattttc aacgatttgc ggtgcctggc cgggatagct ggttttaata
     9061 atcacctgca catcggagag atccggcaac gcatccactg gcgtattaat gatggtccag
     9121 gtgccccaga tgctcagaaa caacgcgccc atcagcacca aaaaacggtt cgccaccgag
     9181 cgacgaataa tccattcaat cattggttat tccctcaatg cgcatgggta gcactttcag
     9241 agcgcatccg ctccagtgcg ccagaaatat tggcttcgga atcaatcagg aacaggccgc
     9301 cggaaaccac cttttcacct tccgccagac cagagcgtaa tgcggtgacg ccttgcgatg
     9361 cctggaaaac agcaacgcgt ttcggtacaa agcgcccgtc ggcatcaacg gtaatcaccc
     9421 gctgttcgtt gccggtatca atcagcgcct gtgacggaat gagcagcatc ggttcgctgg
     9481 cggtgttgag ttgcaaccag gcgttcatgc ccggttttag cgcctcgtcg gcgttgtcga
     9541 cttccagacg cagctgcagc gtgcgggtcg cggcatccac gccaggtagc agcgtccatt
     9601 tgcggatggt gagtgtttta tccggtcgcg ccggaacggt gagggtaaac tgcgaggcat
     9661 ctttcaccag ccaggcgatg gactccggga tcgcagcagt gacccacacc gggtccatac
     9721 cctgaattcg cgattacgcg caatccccct gagaaccgcc atgaacaagg gtttcatgat
     9781 ttgtgatttt agtttggtac gcaatttggt acacaacact attttcactt cagcgggtag
     9841 tcatcaaact caccataacg agcatcgttg atgatgtatg tgatcaccac aaatatcaca
     9901 tctggactag ttccgtcttc atgcgcaggt agactggccc cctgaatctc cagacaacca
     9961 gtatcactta tttaagtgat agtcttaata ctagttttta gacttgtcat tggagaacag
    10021 at
//
LOCUS       DAETQL010000127         9934 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.127, whole genome shotgun sequence.
ACCESSION   DAETQL010000127 DAETQL010000000
VERSION     DAETQL010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.127"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="K0W65_003630"
     CDS             complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="K0W65_003630"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HBI3096733.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="K0W65_003631"
     CDS             complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="K0W65_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HBI3096734.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="K0W65_003632"
     CDS             complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="K0W65_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBI3096735.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4160..4885)
                     /gene="phoU"
                     /locus_tag="K0W65_003633"
     CDS             complement(4160..4885)
                     /gene="phoU"
                     /locus_tag="K0W65_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBI3096736.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4900..5673)
                     /gene="pstB"
                     /locus_tag="K0W65_003634"
     CDS             complement(4900..5673)
                     /gene="pstB"
                     /locus_tag="K0W65_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBI3096737.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRVEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5765..6655)
                     /gene="pstA"
                     /locus_tag="K0W65_003635"
     CDS             complement(5765..6655)
                     /gene="pstA"
                     /locus_tag="K0W65_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBI3096738.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6655..7614)
                     /gene="pstC"
                     /locus_tag="K0W65_003636"
     CDS             complement(6655..7614)
                     /gene="pstC"
                     /locus_tag="K0W65_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBI3096739.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7701..8741)
                     /gene="pstS"
                     /locus_tag="K0W65_003637"
     CDS             complement(7701..8741)
                     /gene="pstS"
                     /locus_tag="K0W65_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBI3096740.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8989..>9934)
                     /locus_tag="K0W65_003638"
     CDS             complement(8989..>9934)
                     /locus_tag="K0W65_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3096741.1"
                     /translation="GYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIYY
                     TSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYN
                     CNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADV
                     YIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGI
                     LNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTVY
                     GEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2562 a         2586 c         2293 g         2493 t
ORIGIN      
        1 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat
       61 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt
      121 catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc
      181 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg
      241 gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
      301 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat
      361 tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg
      421 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt
      481 gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg
      541 ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
      601 agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac
      661 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
      721 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
      781 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata
      841 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
      901 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc
      961 accttccacc tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt
     1021 cataattaac tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat
     1081 ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg
     1141 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     1201 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     1261 tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     1321 ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc
     1381 aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc
     1441 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     1501 tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact
     1561 ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc
     1621 aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt
     1681 gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc
     1741 ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc
     1801 atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac
     1861 ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat
     1921 aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat
     1981 acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc
     2041 cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat
     2101 catttcgctt atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac
     2161 catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag
     2221 tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga
     2281 gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg
     2341 cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc
     2401 agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa
     2461 cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt
     2521 ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag
     2581 accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc
     2641 tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac
     2701 cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc
     2761 caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc
     2821 ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc
     2881 cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg
     2941 cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt
     3001 tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt
     3061 ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac
     3121 gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt
     3181 aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa
     3241 gaatacgcca ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttctt
     3301 cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg
     3361 tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa
     3421 taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg
     3481 cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt
     3541 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
     3601 cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca
     3661 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
     3721 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
     3781 caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
     3841 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
     3901 aaaaccgact tcaccagtat tctctggtta tgtcaggttt tgcctgcgaa tgcagtaaca
     3961 atccagtcat ttattaatgg tttttataac gaacatccag gttcggaaat taatttaatt
     4021 gcgtgcagag aaaatagcaa tgcgctattg ataaaaatat gaccatgctc gcagttatta
     4081 actttgtgta attttaggaa tttataaagt tatatataac aaatcccaat aattaagtta
     4141 ttgggatttg tctggtgaat tatttgtcgc tatcttttcc cgccagcagt ttatccagct
     4201 catcaccgcc tacatgacgg aaatcctgac ccttcacgta gtagaagata aactcacaaa
     4261 tattctggca gcggtcgccg atacgttcga ttgaacgcgc acagaacagc gcggtaagta
     4321 cgctcggaat agtgcgcgaa tcttccatca tgtaggtcat cagttgacga acaatacctt
     4381 cgtattcctg atcgactttt ttatcttcac gataaatacg taccgcttcg tcaatgtcca
     4441 tccgcgcgaa cgcgtccagc acgtcgtgca gcatctggat ggtatgacgg cccagcgact
     4501 ccagacttac cagcaacggc tgatgctgct gggagaattt ctccagcgca gtacggcaga
     4561 ttttgtccgc cacgtcgcca atacgctcca gctcggcaat ggttttactg atcaccataa
     4621 ccagtcgcag gtcgctcgcc gtcggctgac gtttggcgat aatgcgcacg caggcttcat
     4681 cgatcgccac ttccatcatg ttgacgttct tgtcgccttc gatcacgcgc ttcgccagat
     4741 cgctgtcctg gttatgcatc gcggtgattg catcagaaag ctgctgctcc accatgccgc
     4801 ccatggtcat cacctgcgtg cggatacttt ccagttcggc gttgaactgg ccggaaatat
     4861 gtttattaag attgagactg tccataacgc actcctgaat caaccgtaac gaccggtgat
     4921 gtagtcttct gtttgtttct tcgctggctt ggtgaacaga tcgtccgtgt tgctgaactc
     4981 aatcaattcg cccaggtaca taaatgccgt gtggtcggaa caacgcgcag cctgctgcat
     5041 gttgtgggtg acgatcacca cggtgtaatc ctgcttcagt tcggtgatca gctcttcaat
     5101 acgcccggta gagatggggt cgagcgccga acacggttcg tcgagcagca gcacttccgg
     5161 gcgaatggcg ataccacgcg caatacacag acgctgttgc tgaccaccag agagagagta
     5221 accgctctgg tgcaatttat ctttggtttc gttccacaat gcggctttgg tcaatgccca
     5281 ctgcacgcgc tcgtccatgt cggcacggga gagcttctca aacagacgaa cgccaaaggc
     5341 gatgttgtcg tagatggaca tcgggaacgg cgtcggtttc tggaacacca tgcccacttt
     5401 cgcacgcagc agggcgatat cctgagagtt ggtcaggatg ttgtcgccat caagcagaat
     5461 ttcaccttcc acacgctgct ccgggtacag ttcaaacatt ttgttgaagg tacgcagcag
     5521 cgtcgattta ccgcagccgg acggcccgat aaacgccgta acctggtttt tggcgatatc
     5581 caggttgatg tttttcaggg catggaattt gccgtagtag aagttcaaat tacgaacctg
     5641 aattttactc ggggcagttt caaccatact cattgcaatc ttttcctcat cgtacctgat
     5701 gcttttcgct aatcaggctt accgtagggc cgggtaaggc gttcacgccg catccggaaa
     5761 agtgttaacc gtgtttattc ttcgcaaaaa caacgcgcgc cagaatgttc agcagcagga
     5821 cgcacagggt aatgatcaat accccggccc aggccagttg ttgccattcc gcaaacgggc
     5881 tcatcgcaaa cttaaagatc gtcaccggca ggttggcgat cggctgcatc atgtccgtgc
     5941 tccagaactg gttggagagc gcggtaaaca gcagcggcgc ggtttcaccg gcaatacggg
     6001 caatcgccag caggataccg gtcataatcc cggacaccga cgctttcagc gtaatcgcgg
     6061 agatcatctt ccacttcggt gtacccagcg cataagccgc ttcacgcagg ctgtccggca
     6121 ccagtttcag catgttctca gtggtgcgga taacgatcgg cacctgcaac agcgccagcg
     6181 caattacgcc cgcccagccg gagaagtgct ccatctgtgc caccacgatg gtgtagacaa
     6241 acagaccaac cacaatcgac ggcgcggaga gcagaatgtc gttaatgaag cgaatcactt
     6301 ctgccagcca ggatttacga ccatattccg ccagataaat ccccgccata atgcccagcg
     6361 gcgtaccgaa taccgtggcc cacaaaatta acagcccgct acccgccagg gcgttcgcca
     6421 gaccaccacc ttccgtattg ggcggcggcg tcatttcagt gaacagcgcc agcgacatac
     6481 cgtcgatacc gcgagtgatg gtggacatta aaatccagat cagccagaac aggccgaagg
     6541 ccatcgtcgc catcgagagc gtcagcgcaa tacggttttt gaggcggcga cgcgcctgca
     6601 ttttgcggcg agattcagcc agcgccgcag tggtttgcat ttcaaccata gccattagcg
     6661 tgccccctca ttcttagcca ggcgcataat cataaacttc gatgcggcga ggacgatgaa
     6721 ggtaatcaca aacaggatca ggcccagttc catcagtgcg gcaacgtgca gaccggattc
     6781 cgcttccgca aattcgttcg ccagcgcaga ggtgatacta ttgcccggca tgtacagcga
     6841 ggcgctgtcg agctggtagg tgttaccgat gataaaggtc accgccatgg tttcaccgag
     6901 cgcgcggccc agccccagca tgatgccgcc gataacacca tttttggtga acggaagaac
     6961 gatacgccag ataacttccc aggtggtgca gccaataccg taggccgact ctttcatcat
     7021 caccggggtt tgttcgaaca catcacgcat taccgccgca atgtacggaa taatcatgat
     7081 ggcgaggatc acgcctgccg cgaggatacc gataccaaat gcggggccag agaacagcgc
     7141 gccaacaatc gggatattcg acatgatatt gccgaccggc tcctgaaagt aaacggcgaa
     7201 cagcggcgca aagataaaca ggccccacat gccgtaaacg atacttggaa tggctgccag
     7261 cagctcaatg gcgataccca gcgggcgttt cagccagcca ggcgcaagct cagtcaggaa
     7321 cagggcgata ccgaaactca ccgggacggc gatcagcagc gcgataaacg aagtcaccaa
     7381 cgtaccgtag atcggcacca gcgccccgta gatatcgttc ggtgcatccc actctttggt
     7441 ccataggaaa gccagaccaa atttctgaat gctcggccag gaggagatga tcagagagac
     7501 aataatgcca cccaacatca atagcacaat cagcgccgcc agttttacca gcacgctgaa
     7561 aattatgtcg ccctttttac ccggtgggtt aaaagcgggc ttggttgcag ccataagtta
     7621 ctcttcagtt aaacgcgttt acaaagtcat tttgtaatgc cggatgcggc gtaaaacacc
     7681 gtacccggcc tggagtttta ttagtacagc ggcttaccgc tactgtcttt aatattggtc
     7741 ttccacgcag cgcgaacctg ttcaactaca ctatccggca ggctggcgta atccaggtcg
     7801 ttcgcctgtt tagccccggt tttgtacgcc cagtcgaaga atttcagcac ctctgtgcct
     7861 tgttctggtt tcttctgatc tttgtggatc agaatgaacg tggtagaggt aataggccat
     7921 gcatcttcgc ctttctggtt ggtcagatcc tgagcgaagg ttttgctcca gtctgcacct
     7981 tttgctgcat tagcgaagtt ttcttcggtc ggactaaccg gtttaccatc agcggagatc
     8041 agtttggtgt acgccaggtt gttctgcttc gcgtaagcat attcaacata accaattgca
     8101 cccggcagac gctgaacgaa cgcggcgata ccgtcgttac ctttaccgcc cagaccgatc
     8161 ggccatttta cggtagagcc agtaccaacg ttgtttttcc actcttcgtt cactttcgcc
     8221 aggtagctgg tgaagacgaa ggaagtcccg gagccatctg cgcggcgtac tacagcaatg
     8281 ttttgtgaag gcagtttcag acccggattc agtttggcga tggcttcatc atcccacttc
     8341 ttgattttgc ccaggtagat gtcgccgagg gttttaccat ccagcaccag ttcgccagac
     8401 ttcagccctg gaatgttaac cgccagcacc acgccgccaa tcacggtcgg gaactggaac
     8461 agaccttcct gagccagttt ttcgtcagac agcggcgcgt cagaggcacc aaaatcaacg
     8521 gtattagcga taatctgttt tacgccaccg gaagaaccga taccctggta gttaacttta
     8581 ttaccggttt ctttctggta agtgtcagcc catttggcat acaccggcgc agggaaggtt
     8641 gcacctgcac ctgtcaggct tgcttctgca aacacagaga aagcactcat cgataaggtc
     8701 gcggcgacaa cagttgcgac ggtggtacgc ataactttca taatgtctcc tgggaggatt
     8761 cataaagcat tgtttgttgg ctacgagaag caaaatagga caaacaggtg acagttatat
     8821 ataaggaata tgacattttt atgacagaaa ggaattacca aataacttac tgattttaaa
     8881 tgtgaagatg aaaattccac atgccatctt tttgtcatga tgcatttaaa agtgataaag
     8941 aaatgggtga gatgacaacg atgtgatgaa caatcgttgt cagacctatt agcgcacaat
     9001 ctcaacagtt atcgtcgctg ttgcagtaaa ttgccccggg gctgggcgag ctccagtaag
     9061 gctcgcagga gcagctgaaa aagttacact acctgatttt tcaccataca ctgttgattt
     9121 atccatgtct gttggcaact caccaccgtt cactttgatt tccttattat ttttgtcgta
     9181 aatttttatc cccacgttag agtttgaagt tacgaccatt gaattgtccg tattgggttc
     9241 ggtagcataa aaagacgtac tgatatcatc ggtattcagt atccccaggt tttcacactc
     9301 aatagtgact gttttagtaa ttttatgtgt tgttaccgca cttccaacaa cagatgaaaa
     9361 atctgcagct ggtatttctt taaaatcaaa acaaactggc tttaaattat ttatttcgca
     9421 actttgcgga gctgtaatat cgccccgaat ataaacatca gccatcggct gtgagtaatc
     9481 caccggggtg tcacggctaa ttgttccata caatgtcact atctttgttt caggaataat
     9541 caattttcct gcaaatgtct tcttgatata aaaatatact atcgctttag ccccactgga
     9601 tatacttaaa ggttcaatgc ggttgcaatt gtactttgta ttaccagacg ggttgttagc
     9661 atgatattca aaaggtgcat tcacataccc aaccccaaga atatctatag caatcccaac
     9721 atcaaggttc ggattaagat cataataatt aatattagag tgaaccccag cataagttaa
     9781 tgcctggttt atttttgacg tataatagat ataggggaat tctcctacgt taccagtatc
     9841 acaatgacag cgtccaagat atgaggatcc attagttaca gtggtgttaa atgtcgttcc
     9901 aaccttattt tcgttcgcgg taataagata tccc
//
LOCUS       DAETQL010000128         9774 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.128, whole genome shotgun sequence.
ACCESSION   DAETQL010000128 DAETQL010000000
VERSION     DAETQL010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.128"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="K0W65_003639"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="K0W65_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBI3096742.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="K0W65_003640"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="K0W65_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBI3096743.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="K0W65_003641"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="K0W65_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBI3096744.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="K0W65_003642"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="K0W65_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBI3096745.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="K0W65_003643"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="K0W65_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBI3096746.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="K0W65_003644"
     CDS             complement(6031..6360)
                     /locus_tag="K0W65_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBI3096747.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="K0W65_003645"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="K0W65_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBI3096748.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="K0W65_003646"
     CDS             7998..8171
                     /locus_tag="K0W65_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBI3096749.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..9479)
                     /gene="kch"
                     /locus_tag="K0W65_003647"
     CDS             complement(8226..9479)
                     /gene="kch"
                     /locus_tag="K0W65_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBI3096750.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2563 a         2285 c         2433 g         2493 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat caagcaaatt gaccgacata
     9361 ccatagattg ttttaaaaat aagcaatcca tttaaaaaga cggccagcgc gagaatgtcg
     9421 tgccgtaggg tcacccataa atttgttgct gtttgtttga atgtagccca gtgactcact
     9481 gatacctctg taaccacaat tttgtttaat tatcttagcg tgcacatttt ctgaacatac
     9541 atgcagcgtg tgaatgtgtt aaacaatagt agatgacttt atccgcagta atcaaaggtt
     9601 ttgagtgagc aactctgcgt caggaaaggg cttttttcct gaacatgctc acatcatata
     9661 aagaaataca gataaagtta ttatctgctt gtggtagtga atgcactgac cggctataag
     9721 gaaaggccaa acaagaacac ggttgcaaaa accgtgccct taaatattgg atct
//
LOCUS       DAETQL010000129         9720 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.129, whole genome shotgun sequence.
ACCESSION   DAETQL010000129 DAETQL010000000
VERSION     DAETQL010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.129"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..594)
                     /locus_tag="K0W65_003648"
     CDS             complement(<1..594)
                     /locus_tag="K0W65_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HBI3096751.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQN"
     gene            complement(661..1386)
                     /gene="fimC"
                     /locus_tag="K0W65_003649"
     CDS             complement(661..1386)
                     /gene="fimC"
                     /locus_tag="K0W65_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HBI3096752.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVINNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1423..1962)
                     /locus_tag="K0W65_003650"
     CDS             complement(1423..1962)
                     /locus_tag="K0W65_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI3096753.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2027..2575)
                     /gene="fimA"
                     /locus_tag="K0W65_003651"
     CDS             complement(2027..2575)
                     /gene="fimA"
                     /locus_tag="K0W65_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HBI3096754.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3056..3652)
                     /gene="fimE"
                     /locus_tag="K0W65_003652"
     CDS             complement(3056..3652)
                     /gene="fimE"
                     /locus_tag="K0W65_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HBI3096755.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4130..4732)
                     /gene="fimB"
                     /locus_tag="K0W65_003653"
                     /pseudo
     CDS             complement(4130..4732)
                     /gene="fimB"
                     /locus_tag="K0W65_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            6186..6902
                     /gene="nanC"
                     /locus_tag="K0W65_003654"
     CDS             6186..6902
                     /gene="nanC"
                     /locus_tag="K0W65_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HBI3096756.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6922..8028
                     /gene="nanM"
                     /locus_tag="K0W65_003655"
     CDS             6922..8028
                     /gene="nanM"
                     /locus_tag="K0W65_003655"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBI3096757.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8093..9073
                     /locus_tag="K0W65_003656"
     CDS             8093..9073
                     /locus_tag="K0W65_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBI3096758.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            complement(9542..>9720)
                     /locus_tag="K0W65_003657"
     CDS             complement(9542..>9720)
                     /locus_tag="K0W65_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3096759.1"
                     /translation="LKGKLHVISKRYTQRIERHNLNLRQHLARLVRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
BASE COUNT         2834 a         2125 c         2039 g         2722 t
ORIGIN      
        1 attctgtaca ctatttccgc tgaaattata attgagcaat ccggcattaa taccgggatc
       61 ccataactca ggaggaatat aaccacgcgc gcgattactc ataaatgcct gagggatcgt
      121 caggttcagt cgctgctgac caacatccag atgcgcagta gcgtcctgga ccattgtggt
      181 taatggcaca caggcatcat ccgccagcag attcataccg gcgacagaag ccgtattcag
      241 ccccatactg gcgagttgcg cgcgtgtcag gcagggaaca atcccttgtt cactgtcgcc
      301 cgtattaaat gtgacatcac gcgttgccat ataaccatta ttcaaataga tatcgacgcg
      361 atacgtccct ggcggtaatt cttgcccatt ttcaaaacgc gataaatcgg ccacagcctg
      421 gggatcatcc gctaaaaagc gcagattaaa atagaggtcg gcagatgaca aaggtgcctg
      481 tgcggcaaaa gcacaggcga caacgagtcg gacaaaaaaa ccagccaaac gatgcttacg
      541 aatatgcaag cattgtgtgt ttcgctggta aagtcttaaa ttcagatatg acattattcc
      601 tccggtttaa aatcacccgg cagtcaattc tttttattca ggcgaaaaat tccccctgcg
      661 ttattccatt acgcccgtca ttttgggggt aagtgcgcca taatcattta ttgttcggta
      721 agtaatattg cttcctgcat cagaaggcaa tttaaccgtg ctttcgccca ttggaggcac
      781 caatgcattt tcaagaaccc gggttccggc attcaactct gttaccgtca ggtaataggg
      841 tgtcgggtta atcagcgtca gagaattcgc gctacgacga aatcttaatt tttctgcggc
      901 ctgatcgggt ggcaacgcta atttagccgg gcgatagtac agtttaatgc ggctgataat
      961 tgcgagctgt agcatattct cagtcaattt tgatttatcc attgacggaa tcgctttaac
     1021 gttcatccag aataaacttt cccggtcctg tggcaattgg ttatttgttg catcaagaat
     1081 acgtaaggta ttctcttttt ttcccttcat cgcaaacaga ggaggcgtca cgataaaacg
     1141 accatccttt acaccatcgg cattttccac ccatgattga attaaatagg tactattttc
     1201 atcattattt atcacggcaa gttgcacttg tttttgccct gccggataaa ttacgcgagt
     1261 cgcacctaag gccactcctg cttcagcgcg tccggcaacc atcattgcca tgaacatcag
     1321 gatacctgcc agcaagcaga atgttatttc ctgcgatttc cttacattga cgtttttatt
     1381 actcactgtc ctgttcctgt tatcacatta tctactgaac aattactgat aggttaaaga
     1441 gaaccaggcc tgggcattgg cgatgccgcc agtaacccga cgcccggtag cacgatattt
     1501 ggcgatgaaa tgtagcgaag tagagcctga gtaaagccgt ttccagtttg ctggaggacg
     1561 attaatcggt acgaggtttc cttcatcatc aaacaacgct acaccaatat tggaggctat
     1621 ccctggcccc tctcccacgg agagcacatc cggattttta ccatccgcga caccgtgaaa
     1681 cgccacaccc acacgttcac tcaccaccgt gctacattcc cgtaaatgaa taacaaaagg
     1741 caccggtgcg ctatcttccc ctgccgcatg aaaccggtta ctgctgattt gccccatatt
     1801 gaccgtcatt tgtttatcac cggcttcaat ccggcaagtt tccgcaataa tgacgccctg
     1861 aaattgcata tttccgccgg gcagcgtggt attccattta tttccggcca gagcaaacat
     1921 tggcagcaac gacgctaaca gaaataatct cttccttttc atcgttattt ttatcgcaca
     1981 agggtgggca tccctgcccg taatgacgtc cttgaacctg ggtaggttat tgatactgaa
     2041 ccttgaaggt tgcatccgca ttagctgcac ccggggttgc ctcgccgatt gcataataac
     2101 gcgcctggaa cggaatggtg ttggtaccgt tattcagggt tgtttgtgca ctaaatgtcg
     2161 caccgtccag cgtcagagca ttgcctgttc tgtctaggat ctgcacgcca acgtttgttg
     2221 cactacctgc agcagaactc tgcagagcca gtacatcagt acgcgtagca tcaattgccg
     2281 tacctaagaa ggcaacagcg gcttttgtgg caacagtggt atcgcaatca ttcagctgaa
     2341 tgttaaaacc aacggcagag ctggttgctc cagcctgctt caggctagcg gtacgaacct
     2401 gtcccaactg aacggtttga tcaacagagc ctgcatcaac tgcgcaagcg gcgttaacaa
     2461 cttccccttt aaagtgaatg gtcccaccat ttaccgtcgt agtatcggcc agagccgctg
     2521 cggaactgag ggacagagcc gacagaacaa cgattgccag agttttaatt ttcatgctgc
     2581 tttcctttaa aaaaactatt tctaaatcga catgggcagt cgttctgtac actttgtttt
     2641 gtcaacgagt ttgactgcca acactgcaca gttttccccc aaaagatgaa acatttgggg
     2701 ccattttgac tcatagagga aagcatcgcg cacaaacttt ttcagtttat ttgttggctt
     2761 aatattctat tattatcttt atttatagat ttttatattg catgaggtgg tttttggaga
     2821 gaagaatgag gaagatgcgt cgagccacag aaacgttagc tttacatata gcggaggtga
     2881 tgtgaaatta atttacaaga gaaataattt acatatcaaa tagttagatg ctttttgtcg
     2941 ttttttaata tttttatgct tgagaaaaaa tacgtaactt atttatgata tggacagttt
     3001 ggccccaatt gtcttgtgtt tatttgtttt ttaactttat tatcaataag ttaaatcaaa
     3061 cctcttctct ttttaatttt tcgtttatga gattatttct ttcccataat ccggcaaaac
     3121 gagcagcatt actggcggta taacgcacag tatggcgaat atttcgatgc ccgagataat
     3181 cctgaattaa acgggtatct gcaccacgct ccgccagttc ataaccgcaa gcatgcctta
     3241 acatatgagg atgagtctgc gttacggttc cagcttcaat accggcatcg cgaataatgc
     3301 gataggcctg ctggcgagaa agccgactcc cgcggcgaga aataaatata gcgtcggtcc
     3361 ggtcagcgcc tttccagtta gcacgttcct gggtccagcg ttccacggct tcacgctcat
     3421 caaaacgtaa cgggtgaacg gtagaaaatc cgttcttcag tcggcgaata tttattcgac
     3481 cttcattaag gtcaaggtcc tgataatgca gatcaagcag ttcactaata cgcatcccat
     3541 gccgatatgc caacagaata agacaataat ctctggctcc cgttgccccg taacaaaccg
     3601 cctgcatcat ggcctgaact tctttaccgg taagataacg acgtttactc acaatagtag
     3661 tactcctaac tgagatatat ttgaatgaat acctatagga aacctcaatc ggtcaaaatt
     3721 agcccaatag ataaaaagat aaccacacac gaagttatgt ggctatcagt caattacatt
     3781 aaacatcata caaaaataaa atatcactga tgtgacaaca tctattttca ttgatttgat
     3841 tcaatataac aatagattac agaggattaa cttccagtta cttaaaaaat aaagaaaaaa
     3901 gtgtggaaac aggagaattt aagtgatgtg taaacttatg aatgattttg tttatttatt
     3961 ttgaagaaca taaatcatta gattttgtat gggtatggtt aacttaatgt ttattatgat
     4021 aattaagaat cacacttaca ataacatcaa ttaataaatg gttaatcatt tttgatatat
     4081 cgtaagaata atgtagtttt taacaccatc cctggtatct caactatctc tataaaacag
     4141 catgacgctg tcgtcctctg gctctatccc agatgccgta aaaacgccct gcattgctgg
     4201 cggtatacca gacagtatga cgaatattgc gatgcccaag ataatcctag ataagtcgcg
     4261 tatctattcc catattcgcc aaagcaaaac cacacgaata gcgtaacatg tgcggatgaa
     4321 tctccagtga caacccggca ttaccaccag aagtcgagat aatatggtaa aactgttgcc
     4381 gagaaagcgg attcccctta cgtgataaaa atacccactc gctctcagca tgcggataag
     4441 aagtacggat actcaaccag ttttttaaag cctgaacttc tttgttcaat agcgggtgcg
     4501 ttgttgaaaa gccttttttt aatcgatgga tatatataca ctttgcttta agatcaatat
     4561 ccgaaatcct caatcgacaa atttcactcg cccggaaacc atgaataaaa caaagcaaaa
     4621 tcagacaata attacgtgtt gcatgaggcc cggtatttgc tgctttaagg agtgactcga
     4681 tttcactatg ggtaaggaag ttcctttttt tgttatcagc cttattcctc atcgttttcc
     4741 cttataatta cagacgcgca ctagctgtgc tgggttagca ataatccaac atttttatcc
     4801 tgattatgtt tataaaagcg aacgtttgct taataactta cgtaagtgga ccagttcttc
     4861 tgagcgaact taaatggagt agcgatagtt acttataata actttgccat acaaacgcca
     4921 tgctcttgca tgctatgtac ctttatatat ttatcaatct gatacgaatc taataataac
     4981 cctcatcatt aatgcaacta atcttatcta tatatcatgt gatatgttct gtaacaagta
     5041 aaatcaacat aaaaaattca gcaaaattta atatttgtta aactagccgt ctcaaatatg
     5101 acattattag ttaactgcat ttgccacaaa atggctattt catggaacgc agtaatttct
     5161 tagtggctat tatcatgcta attcttagat ccctagcgat tattcctgcc tggttcgtca
     5221 gcttaggcca tgagattgtt acattccttt ttatcctgga gatatggggg aacgcaggcg
     5281 acgctttttc atctacatcc gttggtaaat atagctttgg tcactacgac ttaagccttc
     5341 ctcaatcaca gctattcgcc gggcgatacg gcaaacaaac taagtgaaac atcatcctga
     5401 aaacaccaac atcaacaagc ctctccagat cgacatcagg agtgaccagt tacaagccac
     5461 aaaccaagaa acatatttca tgatgagaat tatgctcagc ctgatggcga gtaaaccctt
     5521 attttcacca ctctcactga gttagcttgc aaagctttct ttgtttagat tctaaataaa
     5581 ttatttataa aacgtatagt taaaagaaaa tctttagttt ttgaagacca ccgcaagtgt
     5641 tcgtctggct tcacatggca tcttcttctt agaaaaagat cgacatattt tgtgacgcga
     5701 attgcaaatc tggttttgtt gtatggattg cgtgattttt gatctggtat aacaggtata
     5761 aagatgcacc acgatagtca atgagactgg gcatctcgta atctatggca aacatcactt
     5821 cagttctttc acatcgggtg atgaaaatgc acttcagtct gaaaggaata tgaaaatgag
     5881 atcaacagac actctatttt atgactctgg gtaaaatggg ttgagtaagt gatataactt
     5941 acgaatattc aaatcaatta aaaatcagaa gagattttat actcaggtat ttaatctgga
     6001 tctctgttta tttaaataat gttaaaagag aattttcaca ggagacctta tacaaaaaat
     6061 ataaaataca gttatccgtt gccaaagaca ctataagcct ggcaaaaaaa tattacacaa
     6121 cataaatgct aattgtttat gcgggctttg tattgctttc tgtatcctac aaatgagtga
     6181 aatttatgaa aaaggctaaa atactttctg gcgtattatt actgtgcttt tcgtccccat
     6241 taatttctca ggctgcgaca ctggacgtac gtggtggata tcgtagtgga agccacgcct
     6301 atgagactcg actcaaagtc agtgagggat ggcaaaatgg atggtgggca agcatggaaa
     6361 gtaatacctg gaataccatt catgataata aaaaggaaaa tgccgcactc aatgatgttc
     6421 aggttgaagt taattacgca attaaacttg atgatcaatg gacggtgcgc ccgggaatgt
     6481 taacgcattt tagcagcaac ggcacacgct acggacccta cgtaaaactg tcctgggacg
     6541 cgacaaaaga tcttaatttt ggcattcgct atcgttacga ctggaaagct taccgacaac
     6601 aagacttatc cggtgatatg tctcgtgata acgttcatcg ttgggatgga tatgtcactt
     6661 accatattaa tagtgatttc accttcgcat ggcaaacgac gctatacagc aaacagaatg
     6721 attatcgcta tgcaaaccac aagaaatggg cgacggaaaa tgcctttgtt ctacaatacc
     6781 atatgacgcc cgatattacg ccatacatag aatatgacta ccttgaccgc cagggtgttt
     6841 acaacggcag agataattta tcggaaaaca gttatcgcat tggtgtgtca tttaaactgt
     6901 agtagacagg agacagtcac aatgaataaa actataatgg cgcttgctat catgatggct
     6961 tcatttgccg caaacgcgtc tgtattaccg gaaactcctg tgccatttaa aagtggtacc
     7021 ggagcaattg ataacgacac tgtctacatt ggtttaggta gcgcaggtac agcatggtac
     7081 aagctagata cacaggccaa agataaaaaa tggactgcgt tagctgcatt ccctggtgga
     7141 ccgagagagc aagcaacctc tgcatttatt gatggcaatc tgtatgtgtt tggcggcatt
     7201 ggcaaaaaca gcgagggatt gactcaggta tttaatgacg tacacaaata caaccccaaa
     7261 accaatagct gggttaaatt gatgtcgcac gcgccgatgg gcatggcggg tcatgtgact
     7321 tttgtacaca acggcaaggc ttatgttact ggcggtgtta accagaatat cttcaatggc
     7381 tattttgaag atctcaacga ggctggaaaa gattcaaccg ctatagataa aatcaacgcc
     7441 cactattttg acaaaaaagc agaagattat ttcttcaata agtttctgtt gtcttttgat
     7501 ccctcaacac agcaatggag ttacgctggc gaatcgccct ggtacggaac ggctggtgcg
     7561 gcggttgtga ataaaggtga taaaacctgg cttattaatg gcgaagccaa accaggattg
     7621 cgaacggatg ccgtatttga acttgatttc accggtaata atttaaaatg gaataagctt
     7681 gatcccgtct catcaccaga tggcgtcgct ggcggttttg cggggataag caatgattct
     7741 cttatatttg ctggaggggc cggattcaaa ggttcacgag aaaattacca gaacggtaag
     7801 aactatgcgc atgaaggcct gaaaaaatca tatagcactg atattcatct ttggcataac
     7861 gggaaatggg ataaatcggg tgaattatcg caaggtcggg cctacggagt atcattgccc
     7921 tggaataata gtttattaat tattggcggt gaaactgcag gcggcaaagc ggtgacggat
     7981 tcagttttaa tttctgtgaa ggataataaa gtcacagtac aaaactaacg cttcagggcc
     8041 ccggtaaagg ggccctgcta tcaatttgtc atttgtatta cgcgaaaatt atatgaacgc
     8101 aataatatca cccgattatt actatgttct taccgttgct ggtcagtcta atgccatggc
     8161 gtatggcgaa ggactgccat taccggacag ggaagatgcg cctcattcca gaattaaaca
     8221 attagcgaga tttgcgcata cgcatcccgg aggcccgcca tgtcacttta acgacattat
     8281 tccactgact cactgcccac acgatgttca ggatatgcag ggttatcacc atcctctggc
     8341 aacgaatcat caaacacagt acggcaccgt tggccaggca ctgcatattg cacggaaatt
     8401 actgcccttc attcctgata atgcagggat tcttatcgtt ccgtgttgcc gtggcggatc
     8461 ggcttttacc gcgggcagcg aagggacata ttcagaacgg cacggagcca gccatgatgc
     8521 ttgtcgttgg ggaacggata ctccgctgta ccaggattta gtcagcagaa cgcgagccgc
     8581 actggcaaaa aatccgcaga acaaattcct cggcgtatgc tggatgcaag gcgaatttga
     8641 cttaatgacc agtgactacg cgtcacaccc tcaacacttt aatcatatgg ttgaagcctt
     8701 tcgtagggat ctaaaacaat accattctca gcttaataat attactgacg cgccgtggtt
     8761 ttgcggcgat accacctggt actggaaaga aaatttcccg cattcgtatg aagctattta
     8821 tggcaattat caaaataatg ttttagccaa tattattttc gtcgacttcc agcaacaagg
     8881 tgaaagagga ctgacgaacg cgcctgatga agacccggac gatttaagca cgggatatta
     8941 cggttcagcg taccggtcac cggagaactg gacgacggca ctgcgaagca gtcatttcag
     9001 cacggcagcc cgtcggggga ttatttctga cagatttgta gaagcaattt tgcagttttg
     9061 gcgcgaaagg tgagcgggga gtttattcat gggggatgtc cttgttcgtt ctcagagtag
     9121 acaacaaaaa acaaatgaga tcaaggtaat atctcttcac ttgcgggctc gaacagcagg
     9181 caattgccaa tataataatc tggcacaagg gtgaaaaacg cgcaccgcat aaaccgttgt
     9241 tattgcttac agattagccg gatgccttaa tagacatccg cgccttttcg attatggctc
     9301 ggaaatctac gaacatctac acagtttact ggaacgtttt gggccacagc gttcgcagca
     9361 ccgtcctgat atgccgttct ggcgattata agtcgacgga ttctggcaaa tagacgatga
     9421 tgatatgctt aatgatgttc aaagaaatta ggccattatt atattattgc ttgcattttc
     9481 tttcattcct aaaagcaccc gccattaatc ccaggcgcat tatgcaggta atgactccaa
     9541 cttactgata gtgttttatg ttcagataat gcccgatgac cttgtcatgc agctccaccg
     9601 attttgagaa cgacagtgac ttccgtacca gccttgccag atgttgtctc agattcagat
     9661 tatgtcgctc aatgcgctga gtgtaacgct tgctgataac gtgcagcttt cccttcaggc
//
LOCUS       DAETQL010000130         9681 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.131, whole genome shotgun sequence.
ACCESSION   DAETQL010000130 DAETQL010000000
VERSION     DAETQL010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.131"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(26..214)
                     /locus_tag="K0W65_003658"
     CDS             complement(26..214)
                     /locus_tag="K0W65_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBI3096760.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="K0W65_003659"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="K0W65_003659"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBI3096761.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="K0W65_003660"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="K0W65_003660"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBI3096762.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="K0W65_003661"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="K0W65_003661"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBI3096763.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="K0W65_003662"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="K0W65_003662"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBI3096764.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="K0W65_003663"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="K0W65_003663"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBI3096765.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6242
                     /locus_tag="K0W65_003664"
     CDS             5109..6242
                     /locus_tag="K0W65_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBI3096766.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6331..6864
                     /gene="blc"
                     /locus_tag="K0W65_003665"
     CDS             6331..6864
                     /gene="blc"
                     /locus_tag="K0W65_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBI3096767.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="K0W65_003666"
     CDS             complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="K0W65_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBI3096768.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="K0W65_003667"
     CDS             complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="K0W65_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBI3096769.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="K0W65_003668"
     CDS             complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="K0W65_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBI3096770.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7788..8354)
                     /gene="efp"
                     /locus_tag="K0W65_003669"
     CDS             complement(7788..8354)
                     /gene="efp"
                     /locus_tag="K0W65_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBI3096771.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8396..9424
                     /gene="epmB"
                     /locus_tag="K0W65_003670"
     CDS             8396..9424
                     /gene="epmB"
                     /locus_tag="K0W65_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBI3096772.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2480 c         2466 g         2270 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg
     5881 atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa
     5941 ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca
     6001 gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta
     6061 agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
     6121 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
     6181 aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt
     6241 aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac
     6301 acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg
     6361 acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta
     6421 gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat
     6481 caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc
     6541 ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa
     6601 gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt
     6661 cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt
     6721 tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa
     6781 gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt
     6841 tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg
     6901 attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg
     6961 gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt
     7021 aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata
     7081 acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact
     7141 tccagcagac cagcaataac taagataatc caggacatat caggctccgg aaatttgggg
     7201 ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat
     7261 attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca
     7321 aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc
     7381 agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc
     7441 agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat
     7501 aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa
     7561 catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca
     7621 gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta
     7681 cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct
     7741 gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga
     7801 gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc
     7861 ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta
     7921 tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta
     7981 acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat
     8041 ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc
     8101 atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca
     8161 gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga
     8221 cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct
     8281 tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta
     8341 tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc
     8401 gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt
     8461 tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt
     8521 ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg
     8581 catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga
     8641 gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc
     8701 tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt
     8761 aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg
     8821 taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt
     8881 ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact
     8941 ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc
     9001 ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct
     9061 ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa
     9121 attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga
     9181 taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta
     9241 ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga
     9301 agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact
     9361 ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca
     9421 gtaactgatt aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg
     9481 cctacatcgt gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt
     9541 tacgccgcat ccggcaagag caacaaacgc aagtacagca accactgaga aatttaatct
     9601 ttgcacgttg cataaccttt aattattcat atgcattgct gttgttactt tttatatagc
     9661 taacaccaat aattaacctt t
//
LOCUS       DAETQL010000131         9595 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.132, whole genome shotgun sequence.
ACCESSION   DAETQL010000131 DAETQL010000000
VERSION     DAETQL010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.132"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(7..327)
                     /gene="psiF"
                     /locus_tag="K0W65_003671"
     CDS             complement(7..327)
                     /gene="psiF"
                     /locus_tag="K0W65_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBI3096773.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(446..1861)
                     /gene="phoA"
                     /locus_tag="K0W65_003672"
     CDS             complement(446..1861)
                     /gene="phoA"
                     /locus_tag="K0W65_003672"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HBI3096774.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="K0W65_003673"
     CDS             complement(1962..2222)
                     /gene="iraP"
                     /locus_tag="K0W65_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBI3096775.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2404..2607)
                     /locus_tag="K0W65_003674"
     CDS             complement(2404..2607)
                     /locus_tag="K0W65_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096776.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2685..3779
                     /gene="ddlA"
                     /locus_tag="K0W65_003675"
     CDS             2685..3779
                     /gene="ddlA"
                     /locus_tag="K0W65_003675"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBI3096777.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3803..4015)
                     /locus_tag="K0W65_003676"
     CDS             complement(3803..4015)
                     /locus_tag="K0W65_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBI3096778.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4275..4583
                     /locus_tag="K0W65_003677"
     CDS             4275..4583
                     /locus_tag="K0W65_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HBI3096779.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="K0W65_003678"
     CDS             complement(4642..5736)
                     /gene="yaiW"
                     /locus_tag="K0W65_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBI3096780.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="K0W65_003679"
     CDS             complement(5749..6969)
                     /gene="sbmA"
                     /locus_tag="K0W65_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBI3096781.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7321..8478
                     /gene="ampH"
                     /locus_tag="K0W65_003680"
     CDS             7321..8478
                     /gene="ampH"
                     /locus_tag="K0W65_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBI3096782.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8479..8982)
                     /locus_tag="K0W65_003681"
     CDS             complement(8479..8982)
                     /locus_tag="K0W65_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3096783.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9070..>9595)
                     /locus_tag="K0W65_003682"
     CDS             complement(9070..>9595)
                     /locus_tag="K0W65_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBI3096784.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2381 a         2448 c         2285 g         2481 t
ORIGIN      
        1 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
       61 cagagcttgt tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa
      121 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
      181 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg
      241 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
      301 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
      361 gtttgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
      421 cgaaaattca ctgccgggcg cagttttatt tcagccccag agcggctttc atggtgtaga
      481 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
      541 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca
      601 ccatcactgc gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg
      661 gcgcaacaac ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct
      721 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
      781 tttgcccaca agggttcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
      841 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca
      901 gggttggtac actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga
      961 tattgccgtg gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa
     1021 acaggccaag caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
     1081 cgttcaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
     1141 caccagcggt tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac
     1201 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
     1261 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
     1321 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
     1381 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
     1441 cgccgttata ggttttgaca tcggttgacc aggcggttgc tgatgcagcc gagtcggtga
     1501 cgtagtccgg tttgccagtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg
     1561 gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag
     1621 taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat
     1681 cgctaagaga atcacgcaga gcggcggtct gatcacccgt taagcggcga gcaccgccgg
     1741 gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg
     1801 cttttgtcac aggggtaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca
     1861 ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg
     1921 gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgaca aggatgcttc
     1981 aataacttct ttacgtaatc gcgcagcagc tctgtatcgt cgtcaggaat gctggcatcg
     2041 ggctttacct cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc
     2101 gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt
     2161 tctttcgact cttcttcttt ttgggcaagc ttaaataaca actcagcaat gagatttttc
     2221 atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca caacttcata
     2281 gtgtctggaa gtattatttt tatgacaaag gaaatacttt gcgcaaatat tttttgtttg
     2341 attgccagcg aaacgtttct cttatttaaa taattaccca cacaaaatat aggcttttaa
     2401 ttatcaacct ttattataaa taaaagctat gaaatatatt ctcttcgtta acttataatt
     2461 aagagaaaaa aactcaccgc attgccattt aagccaggaa taaattagct tcccgttcat
     2521 catttttgtg atcttgcgca catctttgag ataacgcact ttcttcacca gtacgttgca
     2581 cgctttgtca gcagaagatt gcaacaatct ttcagaaggc cgcgaatccc ccgttttgtc
     2641 gtggtaatgt atgcactttg tgttagacgg gataggtttt taagatggaa aaactgcggg
     2701 taggaatcgt ttttggtggt aaatcagcgg aacatgaagt gtctctgcaa tcggcaaaaa
     2761 acattgtcga tgccattgat aaaagtcgct tcgacgttgt gctgctgggc attgataaac
     2821 aagggcaatg gcacgtcagc gatgccagca attatctgct aaatgcagac gatcctgccc
     2881 atattgcgtt gcgcccttcg gcgaccagcc ttgcgcaggt gccaggtaaa catgagcatc
     2941 agcttatcga cgcgcaaaac ggtcagccgt tgccgacggt ggatgtcatt ttcccgattg
     3001 tccacggtac gctgggcgaa gatggttcct tgcagggaat gctgcgggtc gccaatttac
     3061 cgtttgtagg ttctgatgtt ctggcttcag cagcctgtat ggataaagat gtcaccaaac
     3121 gtctgctacg cgatgccggg ctgaacattg cgccatttat taccctgacg cgcgctaatc
     3181 gtcacaacat cagttttgcc gaagtggagt ctaaactggg gttaccactg tttgtaaaac
     3241 cggctaatca gggctcttct gttggtgtca gcaaagtaac cagtgaagaa cagtacgcaa
     3301 ttgccgtcga tctggcgttc gagttcgatc ataaagtgat cgttgagcaa gggatcaaag
     3361 gtcgtgagat cgaatgcgca gttctgggca acgacaatcc gcaagccagc acctgtggcg
     3421 agatcgtact caccagcgat ttctatgcct acgacaccaa gtacattgac gaagatggcg
     3481 cgaaagtggt agttccggca gccattgcac cagagatcaa cgataagatc cgggcgattg
     3541 ccgttcaggc ttatcagacg ttgggatgcg caggcatggc gcgtgtagac gtgtttttaa
     3601 ccccagagaa cgaagtggtg atcaacgaga tcaacacact gcctggcttc accaacatca
     3661 gtatgtatcc gaagctgtgg caagccagcg gtctgggtta caccgatctg atcacacgtt
     3721 tgattgagct ggcgctggag cgtcacgctg cggataacgc attgaaaacc acaatgtaat
     3781 attttaacgc ccgataatat tcttattggg cgttttcagt ttcttcatcg cgacgacgaa
     3841 taatcaaccc cgccagccag aaactaatca cccacgtcac cagaccgacg gcataggtct
     3901 gccaaccttt tgcttcaaat cccagtaccc ccaccacgcc attaagaatg aatataaggc
     3961 caatggcgaa cgcatagtag tgccagtcac ggcggatttt tacaggcaga ttcatggcag
     4021 ctccgttaac gaaaaacaac atccttgcat attctgtaac aaccgtctgt ggtgtagaca
     4081 acattttcgt aattattaat aaatttaact taaggttgcc attttgtgac tcacaacgaa
     4141 gattcacgct tcgggaatat tcccaacatc aatttaccat gattctgata ttgacaatct
     4201 aaatgacctg tcagacttgc cataagccgc tggtatcaca ccagaagaga agcagatgac
     4261 ttccggaggt ttttatggct gatttcaccc tgtcaaaatc gctgtttagc ggaaaatatc
     4321 gcaatgcctc ttcaacgcct ggcaacattg cctatgcgtt gtttgtgctg ttttgctttt
     4381 gggctggggc gcaattgctg aacctgttag tgcatgcgcc cggcgtctat gagcgtttaa
     4441 tgcaggtcca ggaaacaggt cgcccacggg tggaaattgg tttaggtgtc ggcaccattt
     4501 tcgggctgat cccgttttta gcaggctgcc tcatttttgc agtggtggcg ctatggctgc
     4561 actggcgaca tcgccgccag taaaatttac gttgccagat gcggcctgaa acgccttatc
     4621 tggcctgaca ttcctgtttg atcactgttt catacaacgc gcccgccgtt cgtctacgcg
     4681 cttcgcaaac caggccgtag tcaggttgcg ggtgattttc gggctttcca gttgaatgcc
     4741 aggcaacatt tcgcggggta gtgatttacc cgttttcgct tcggcaagtt gataaacttt
     4801 cttgtacagc gccgtctcct caaaagagaa gctgtcacct ttctctaact gacggcgaat
     4861 ttcgctgtcg ctcatcccca gttttcccgc cagtttgcgc gttgccagtt cggttttccc
     4921 gggttcttta ctgtcgtaac gaattaaatc accgtccagt gccagcttca cgccgctggc
     4981 cttactgact gcgttctgaa acgcggcatt acggctggcg taccagccag cgttaaaatc
     5041 agcaaagcgg tatatcggtg cgctatagct ggcgggataa ttcagtaaat ggtaagtacc
     5101 aaaccacaac ccgccgcggc gactgaagac ttcctgacga actgtaccgt ccattttcca
     5161 cggataccct ttggtatgct gttcggcaaa agcaatgctg acctgcatcg gcccaccggt
     5221 gcgcaccgga ttaagcgaac caaacagcgt ctgccccatt ggtaccatgc tgatcagatc
     5281 gtcaaaaatc gcgctcaatt gcttttctgt acgcaccgaa tctaaccgtt cgctataact
     5341 tttgccgttt ggcgatttga tttttagcgc ggtatgaacc agaaaagcag gaatgtgcat
     5401 ccgttcagca cgacggtcaa tctcttgcca ggcgatcttg cttaagcccg gaacggccgg
     5461 atctgcctga taatttgact cctgctgcgc caccgccagc accgagtaga cgttttccag
     5521 cgtcggtgcc agcccctgac tttcaaaggt ggtggcgaga tctttcgccc aggcatcgcg
     5581 gtctttcacg ctcgcaggca tcttctgccg cacgacgctc gctacatcta tcgctttttc
     5641 gccctttttc aacggttgcg gagcctgact actacacccc gctaaaacca gaaccgccaa
     5701 caatgacagc gaagatagat gattgacgcg cgacattact tctccttttt agctcaaggt
     5761 atgggttact tcctgaattt tgtcaccatc cagctcatgt tcaaagctgc gcagacgttt
     5821 gtagatagac atcaactcaa ccagtgtggt ccatgagtta atcaggtact ggaaagcacc
     5881 gcgaacctga ccaaaaacgt tggtaatctg cgtcatcagg ccgagcgtaa tcgtaccggc
     5941 aacaattgac ggaaacagca agaacaaacc gaaaacgtta tcgacctgca aatagagaat
     6001 gcgggcgatg ttgaaataca tatagtgaaa atagaggcga aaatagtttt tccgtacggc
     6061 gctaaacagc tcgcgtaccg taggcggcgt cgcgcgcgtg gcatcgtctt caccataaac
     6121 cagctcttta cggtaggcgg cctctactcg ctggttttta aactccagcc ccggcagttt
     6181 gatccctacc actgccagca atccggtccc catcagcgac cagacgatag cggcaatcac
     6241 cagaccatac ggaatgtgcc cgataatcgg cagctccggc acatgcgcgg agagcgttac
     6301 cagcaccggc aggaaggcga tcaacgtcat gatggcgttg ataaaactga cgcccatatt
     6361 ctccagcgtt gaagcaaaac gcatggtgtc ttcctgcaca cgctgtgcgg caccttcgat
     6421 atgacgcagt tgttgccagt tcgccatgta atattcgttc atcgccgtac gccagcggaa
     6481 cacgtagtga ctgacaaaga agttgttcag tacactgatc accaccgcga tcagcgcaat
     6541 ccccagaaag acgcccactt cgcggtaaaa ttgttcgata gtgactttat gcggcgaact
     6601 tagcgcggtt tgaatcagat catagaacgg cgcataccag gcgttgacgg cgaccccgac
     6661 ttccaccaaa aaccaagtga cgaagataat cagcgcagta ccgagtattg accagtattg
     6721 ccaacggtgc gggctgtaga taaaccagaa aaatgcaaaa agtcctacgc aaacaatgta
     6781 gtaagcgtaa aaaatcagga aatccaacga ccagaaacgc gcggcgctaa tcgggatctg
     6841 cccggaagcg ccggtgatac gtgccaccca gtcaccccca ccggcttgcc agaagataac
     6901 ggcaatcaat gcccaaacaa aggccgagag aaaaaacgtt cccggctttg ggaaaaaaga
     6961 cttaaacata tgcactcctg ctaacttctt atcgttttaa caactgctgt gtattaaccg
     7021 catgaccgct acaaagcgtc agccaaatga aaccgcccat tgtgacccat agcaaggcgc
     7081 ttagttcgcc agcctgacca taagaattgt ttcgacggat ttcacaaacc aaagattgtt
     7141 tacataagcg gattaacacg cgcaggatca caaatgcagg cattggtgag caggaaaaag
     7201 atcgtgttta cgcgccgtta cgtttttaac actattattg cactaattcg ccctttgcaa
     7261 tccatcaatg agtagtataa atacgctcag ttaccttcat tcaatctatg gacaccaccg
     7321 ttgaaacgta gtctgctttt ttctgccgtg ctgtgtgcgg cgtcattgac ctccgtccat
     7381 gcggcgcagc cgatcactga accggagttt gcctctgata ttgtcgatcg ttatgccgat
     7441 catatttttt acggcagcgg tgccacgggg atggcgctgg ttgttatcga cggtaaccag
     7501 cgcgtctttc gtagttatgg tgaaacgcga cctggtaata acgttcgccc gcagctggat
     7561 tcggtcgtgc gtattgcttc cctcaccaaa ctaatgacca gtgaaatgct ggtgaaattg
     7621 ctcgaccagg ggaccgtaaa gttaaacgat ccgctaagta aatatgctcc gccaggcgca
     7681 cgcgtgccga cctacaatgg gacgcccatt accctagtga atctggcaac ccataccagc
     7741 gccctgcccc gtgaacagcc cggtggcgcg gcacatcgtc cggtgtttgt ctggccaacg
     7801 cgcgagcaac gctggaaata cctttctacg gcgaagctga aagccgcgcc aggcagtcag
     7861 gcggcgtact ctaaccttgc gttcgacctg ctggcagatg cactggcgaa tgcgtccggc
     7921 aagccttata cccagttgtt tgaagagcag attacccatc cattggggat gaaagacacc
     7981 acctacaccc cctcaccgga tcagtgccgc cgtttaatgg ttgctgagcg cggtgccagt
     8041 ccgtgcaata acacgctggc ggcaattggc agcggcggcg tctattccac gcctggcgat
     8101 atgatgcgct ggatgcagca gtatttgtca tctgattttt atcagcgcag taaccaggca
     8161 gatcgcatgc agacgttgat ttatcagcgc gcgcagttta cgaaagtgat tggcatggat
     8221 gtccccggca aagcggatgc gcttggttta ggctgggtat atatggcccc gaaagagggt
     8281 cgtccgagga ttattcagaa gacaggcggt ggtggcggtt tcattaccta tatggcgatg
     8341 atcccgcaga agaatattgg cgcgtttgtg gtggtcactc gttcgccgtt gacgcgcttt
     8401 aaaaatatga gcgatggcat caatgatctg gtgaccgagc taagcgggaa taaaccgttg
     8461 gttatccccg catcctgatt aatactctga aggcaaacgg ttgatagcga ccagtttgcc
     8521 tttattcatt tcgatatagc cgcctttacg caaagcagcg agaacttctg cgacgacaga
     8581 acgcgatatg cgtgtacgtt gatggatata attcatcaca ccaatacgtg atcgcaattc
     8641 ttcattccag tcaatcattg ataataatgt tgcgcgggtt tgtttggctg gtagatgata
     8701 tcccgtacaa tttccttctg atattaattt gtatggtata tcgtttttca ttaaaccatc
     8761 agccagcccc ataatataag gagcctgggt aataccgata agtacgtttt cttctctacg
     8821 cagagagata actccttcta aaataacgaa agtatcttcg ttattgacat cactggaaaa
     8881 tataacttgc ttttcattat taaactcgaa gcgcgtaccg tatctggaca aacatttatc
     8941 gagcttacca aattcctgaa gaggtttaac tacagataac atttgcgcgt cctttgcagt
     9001 aatgcccgtc aaatccttga cgggcattat ttagattaaa ttaccagtat ttcttcggag
     9061 tgaagaatat taccaagtat atttaacacc cacgttcgcg gaccagtctt gatctacgtc
     9121 accaccaccg aggtagttag catcggtata ggcgctgaag ttcttggtga agctgaactg
     9181 agtacccaga ccaacacgta ccgcagaccc ttcagtaccg ttatcgatgg aatcaccgtt
     9241 cacatcgtta tcgttgttag agtcgtcgta gacgtaagcc agtttgaagt acggagtcag
     9301 agcctgatct tcgctgtagg tgaaggtata acctgcatct acacccagtt cataacgcat
     9361 gctgtcgtaa ggctgaccgt caactttcat gtcgttgctc agctggtagt catcaccaga
     9421 ctggaacaga ccagaaatgc tgccgtaagg agtcacgtaa ccagcatcac ccagtttgaa
     9481 gtcgtaaccg gttttcaaac cgaagcccca agcgtcggag ttggtgctac cgtcaacgta
     9541 agtaccgttg ctcatggttg cagacaggtc gttgttgaag tgagagtagc tcaag
//
LOCUS       DAETQL010000132         9493 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.133, whole genome shotgun sequence.
ACCESSION   DAETQL010000132 DAETQL010000000
VERSION     DAETQL010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.133"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..189)
                     /locus_tag="K0W65_003683"
     CDS             complement(<1..189)
                     /locus_tag="K0W65_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HBI3096785.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLGEKA"
     gene            709..1404
                     /gene="minC"
                     /locus_tag="K0W65_003684"
     CDS             709..1404
                     /gene="minC"
                     /locus_tag="K0W65_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBI3096786.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1428..2240
                     /gene="minD"
                     /locus_tag="K0W65_003685"
     CDS             1428..2240
                     /gene="minD"
                     /locus_tag="K0W65_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBI3096787.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2244..2510
                     /gene="minE"
                     /locus_tag="K0W65_003686"
     CDS             2244..2510
                     /gene="minE"
                     /locus_tag="K0W65_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBI3096788.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2882..3226)
                     /locus_tag="K0W65_003687"
                     /pseudo
     CDS             complement(2882..3226)
                     /locus_tag="K0W65_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3261..3380)
                     /locus_tag="K0W65_003688"
     CDS             complement(3261..3380)
                     /locus_tag="K0W65_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBI3096789.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3341..3526)
                     /locus_tag="K0W65_003689"
     CDS             complement(3341..3526)
                     /locus_tag="K0W65_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096790.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3627..3800
                     /locus_tag="K0W65_003690"
     CDS             3627..3800
                     /locus_tag="K0W65_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096791.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3802..4146
                     /locus_tag="K0W65_003691"
     CDS             3802..4146
                     /locus_tag="K0W65_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HBI3096792.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4150..4485
                     /locus_tag="K0W65_003692"
     CDS             4150..4485
                     /locus_tag="K0W65_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBI3096793.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4542..6863)
                     /locus_tag="K0W65_003693"
     CDS             complement(4542..6863)
                     /locus_tag="K0W65_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBI3096794.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6980..7189)
                     /locus_tag="K0W65_003694"
     CDS             complement(6980..7189)
                     /locus_tag="K0W65_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096795.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7940..9463)
                     /locus_tag="K0W65_003695"
     CDS             complement(7940..9463)
                     /locus_tag="K0W65_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBI3096796.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2659 a         2204 c         2080 g         2550 t
ORIGIN      
        1 ggctttttcg ccgagatatt tccttgttaa ctctggggaa atgccatctg agttcacaca
       61 aatatacggg tcgcaaacaa cgccgcacgt cggagattga agtccgccag cgtcatgtcc
      121 agacgccatt gatgagaaca tcatccccac acctgacaat ccgatataaa taatacgcat
      181 catattcata gcacatcctg ataatccaac cgtacaacac ttatgctcat ttcgtcatca
      241 atccgtaatc aaaaccgctg acctggacga gttacgatga cctgacagaa gtatcagacg
      301 tcttctgcgg tttctatgat agagcaattc tgtggcggat attaagataa cgggtatctg
      361 ttctttaatg atgctgcaaa aaatggaaag ttaagaaagc ctggcgtggt gaaaaaatac
      421 acttgcctca atactggata tttaaacagg tctggagatg ctgtcacgat tagggtgcgg
      481 aaaaaggaag cgtaaataag aagaatcaaa agtacgacgg caatgggttg attgacaagg
      541 gtatttttta agctatgaat cagcgccatt tatcacagaa tagactttta ctctgaataa
      601 atgggagggt gacttgcctc aatataatcc agactataac atgccttata gtcttcggaa
      661 catcatcgcg cgctggcgat gattaatagc taattgagta aggccaggat gtcaaacact
      721 ccaatcgagc ttaaaggcag tagcttcact ttatctgtgg ttcatctgca tgaggcagaa
      781 cctaaggtta tccatcaggc gctgaaagac aaaatcgctc aggcccccac atttttaaaa
      841 catgcccccg ttgtactcaa cgtcagtgca ctggaagacc cggtaaactg gtcagcgatg
      901 cataaggcgg tttcggcaac cggtttgcgg gttattggcg taagtggctg caaagatgcg
      961 caacttaaag ccgaaattga aaaaatgggg ctgcctatcc tgacggaagg aaaagaaaaa
     1021 gcgccacgtc cagctcccgc accgcaggct ccagcgcaaa atacaatgcc ggtcacaaaa
     1081 acgcgtttaa tagatacccc ggtgcgttcc ggtcagcgta tttatgctcc acaatgtgat
     1141 ctgattgtta caagccacgt tagcgctggg gccgaattga ttgccgatgg gaacattcat
     1201 gtctatggca tgatgcgcgg tcgtgcgctg gcaggggcaa gtggtgaccg ggaaacgcaa
     1261 atattttgta cgaacctgat ggcggaattg gtgtccatcg caggtgaata ctggctgagt
     1321 gatcaaatcc cagcagaatt ttatggcaaa gcggcgcgac tgcagttagt cgaaaacgct
     1381 ttgaccgttc aaccgttaaa ttgatccctt tttaacaagg aatttctatg gcacgcatta
     1441 ttgttgttac ttcgggtaaa gggggtgttg gtaagacaac ctccagcgcg gccatcgcca
     1501 ctggtttggc ccagaaggga aagaaaactg tcgtgataga ttttgatatc ggcctgcgta
     1561 atctcgacct gattatgggt tgcgaacgcc gggtcgttta cgatttcgtc aacgtcattc
     1621 agggcgatgc aacgctaaat caggcgttaa ttaaagataa gcgtactgaa aatctctata
     1681 ttcttccggc atcgcaaaca cgcgataaag atgccctcac ccgtgaaggg gtcgccaaag
     1741 ttcttgatga tctgaaagcg atggattttg aatttatcgt ttgtgactcc ccggcaggga
     1801 ttgaaaccgg tgcgttaatg gcactctatt ttgcagacga agccattatt accaccaacc
     1861 cggaagtctc ctcagtacgc gactctgacc gtattttagg cattctggcg tcaaaatcac
     1921 gccgcgcaga aaatggcgaa gagcctatta aagagcacct gctgttaacg cgctataacc
     1981 caggccgcgt aagcagaggt gacatgctga gcatggaaga tgtgctggag atcctgcgca
     2041 tcaaactcgt cggcgtgatc ccagaggatc aatcagtatt gcgcgcctct aaccagggtg
     2101 aaccggtcat tctcgacatt aacgccgatg cgggtaaagc ctacgcagat accgtagaac
     2161 gtctgttggg agaagaacgt cctttccgct tcattgaaga agagaagaaa ggcttcctca
     2221 aacgcttgtt cggaggataa gttatggcat tactcgattt ctttctctcg cggaagaaga
     2281 acacagccaa cattgcaaaa gaacggctgc agattattgt tgctgaacgc cgtcgcagcg
     2341 atgcagaacc gcattatctg ccgcagttgc gtaaagatat tcttgaggtc atttgtaaat
     2401 acgtacaaat tgatcctgag atggtaaccg tacagcttga gcaaaaagat ggcgatattt
     2461 ctattcttga gctgaacgtg accttaccgg aagcagaaga gctgaaataa gcccgctgta
     2521 aaaacgcatt tatcttcaag gcagagtcat ttctgccttg agtttttcat ccctctcatc
     2581 cacgttgtgg taaagcggcg agtattcttg ctgatactcc tcattgctat ttccacctcc
     2641 ccctttacac cttaaggctg ttcccacgca cagaacaaaa tgtttatgcc tggttgagta
     2701 aataaccatg ttattagtgt gtatatactg ggagatgata accccgcaat ttcattattc
     2761 attgttctcc cataggatga ggtttcccgc cgtaactggc gggctttttt tgccccagaa
     2821 ttttctcttt caacatcctg taaacgaaaa ctgcgccgaa gcgcagttta atcgttgatg
     2881 gtcagaacga ccagttcaca ccagccaccg cgttccacgg ggactccaca ccggcaccat
     2941 ggctataccc caccccgaaa tgcccgctta acgtactgct gaatgaggct ttaatacctg
     3001 cctggtatat tccacgtctg cccgacaaat cattgacaaa attaccgtca ctattcactt
     3061 tcacccgatt atcatcgaca aattctttgc gcacagccgc cttcaggcag ggttcaattt
     3121 ccataccgtt ccccagacgc atgttgtaac tcagcgttgc acccagttca cgatatatac
     3181 tgcgggtatc gactgatttc gattccatgc cattggataa atgatagtag cgtctaagtg
     3241 acctaagctc tgctgcaaca tcagcccgca ggcaccagac caggggattc atcctgaaga
     3301 gacagcgcag gtgtattgtg ttcaccgctc accaaagaca tcatgatgaa atgatgatat
     3361 cccgcataag aaagaggcat tttttacacg cagtgcgctg aagtgtagtt ggataaaaaa
     3421 tcactctttt cagataatac tgtcgtacgc ttatctttag acaatgaggc cgtcggaaaa
     3481 ataaaatgac taatgtaatc gttgctgatg aatatcttct gctcacataa aaatcacaca
     3541 ataatattga taaatcagat tatcttacct ttacaacatt gcgttaacag tcaatgacac
     3601 ttcttgaata aaaatattgt caatcaatga gttattcctc atttaagatt attctcattc
     3661 acgtaaaaaa cataattcct atcatcacag ccacgctcat tttgaattat ctgaataata
     3721 gcgagcgttc tttagttaag cagatcttaa ttgaagacga aattattgtg tgtgcaactt
     3781 acttaatacc ggacatctaa gttgaagaaa aaaatacttg ccttcgggct tatctcagca
     3841 ttgttctgct ccacgccagc gatggcggat atgaaccgca ccacgaaagg tgcattgcta
     3901 ggcgcaggcg ttggtttact tactggcaat ggtgttaatg gcgtactgaa aggtgcagct
     3961 gttggcgctg gtgttagtgc agtaacagaa aaaggccgcg acggtaaaaa tgcgcgtaaa
     4021 ggtgcaaaag tgggtgccgc cgttggtgct gtcactggcg ttctgaccgg caatggcctc
     4081 gaaggggcga ttaaaggtgc cgtgattggc ggtacgggtg gtgcaattct gggtaaaatg
     4141 aaataaggaa tgatgatgaa aaaatttgct ttactggcgg gcctgtttgt tttcgcacca
     4201 atgacctggg cgcaggacta caatattaaa aacggtttgc cgtcagaaac ctatattact
     4261 tgcgcagaag ccaacgaaat ggcaaaaact gacagcgcac aggttgcaga aattgttgcg
     4321 gtaatgggta atgccagcgt tgccagccgt gatttaaaaa ttgagcaatc tccggaactg
     4381 agcgccaaag tggtagaaaa actgaatcag gtttgcgcta aagacccgca aatgctgttg
     4441 atcacggcaa ttgatgacac catgcgtgct atcggcaaaa aataatattt catatgtacc
     4501 gcgtcgatga atacgacgcg gttttttagc taacggcggg atcagaactt atatttcagg
     4561 cccaccatca ctgcggtgtc attgtagcca ttatcaccca gctgcacgcc cacattcccc
     4621 cagacgctgg ccgctggatt caggttgcca tttacgccaa gtttaatttc ccccaaatta
     4681 ctcaccccat cttgttcgac tttcacaccg ttcattgaga cagcaaaatc tttactgtta
     4741 tggatccagt tcatttcaac aaaaggttca aagtcgtcac cgtgcggacc gctgtttttc
     4801 tcctgagtac gaataaaggt gcggaagccg agtcgggtct ggatgttgtt atttgcgtca
     4861 ttctcaatac gtgagccatt ggcttccgtg tgcgcattat gatcgacgcc catccagata
     4921 gcctgtgcct gtggctggaa gatccacgta tatttcgccg cttccagacc aaaggattta
     4981 ttcagggcaa aaccgtaacc cgcttccagc gagacggttg cacctttcgc gctataggat
     5041 tcataggcta attcatcccc tttcacggag tttttaaacc agctatattg cgcccaggag
     5101 tcaatgtatg cgcctttttt actgatgtca tcggcaaacc aagtggcata cagccctgcg
     5161 ctatagcctc tgacactccc tttcgaacga taatccgaca cgctggaatg agttaagttg
     5221 tagtcgcggg cataaccagc catcactccc aaacgccaac tgtcactatc ggtaaaacca
     5281 ccggttaaca gatcgccccc taactgcgag acgtagcgat gcgaggtcgt tctcaactgt
     5341 ccgttgctgt cacgccaggc attatgcccg ccaatttgac gtagccaaag tcggcttgaa
     5401 cgctcctgtt cagttacagg atcgatgtag cgcgtttcac ccgcacggtc gttcagacgc
     5461 ataacaaaaa gagtgttagc ggctgccagg ttggcgacgt aacttccagc ttcggggcgc
     5521 aggacggggg taggcgcaac agtaggcgct gaacttcctc cggagtttgt ttcaccgccg
     5581 ctcgttgcct ggaagttagt cagataccag tttttattgg tcgcttcggt attacctcgg
     5641 gttaatgaat attcgtaggc accggcgaaa atcctcgaat ccttaataaa ttccccattt
     5701 gattccccct caacgctgat aatctctatc ccatttaacg tgtaagctcc gctgcctcct
     5761 gcattggtaa ctttaactcg ggttgttcca gaggtatttc cattaataac gactttatcc
     5821 gtagctgaat tatcatcatc caattcagtt ctgagatgca atacgccatt attaccaacg
     5881 taattttcag ttattgttaa tcttgttggt tcgacatctc gtccatctgc ccgggaaata
     5941 tagaccgtac tattatcaac cgtgaggttg ctcaccgttg agtcagcttt aatatcccag
     6001 gtactattat ctgaaagcga cagataggta tgagtgttat catctgttga aatattagct
     6061 gaacccgtta tttttgaatt agttgcatcg acggtcaaat gacctttaat attattgata
     6121 aaaacatctg cagccgatga tacctcacta tccgtaatgg tcacgttact cgtctcatct
     6181 ttatggtaga ggatttcatt tgctgaccgt agtgagctac cagataaaat atctaccgtt
     6241 gcttcctgac catttatgga agactccagc acaattccag acccctgatg cgcgactgca
     6301 gatgtatttt tcagcgtcac cgtgccgcca ttataaattt tagcaatcgc ggcgtcagga
     6361 cccgttgtat aaatttctcc ctggttaaat gaaactgtcc cctggcgggc agcgatggca
     6421 tgagcattgt ccccatgcgt ggtgatatca acatcggtta atgtaacgct ggcattggta
     6481 taatcaagcg tgtcaccagc cttgacgaca gtgccattag cgtacgcggc ataactttct
     6541 gttccgtggg ttgtgatctc cccttttgcc atatccagtt ttgctatggc attattttca
     6601 ttacttcctg tatcggcagt actttcttca tcaagagaag atacacacgc acacgcgctt
     6661 ccgggagatg cacacgcaag aacgccgact gcgtgattac ccgcagtaac aataaatgta
     6721 tcatctccgt cagaaaaaga gggggtaacc gaaccgtcag agttgactct tattatggtg
     6781 tcagtattca ggtttccact cgtttgactt agcaaaccaa ttgagtaatc ccccgttgtg
     6841 acaatcgtgt ttttaccctg caaaataaca gcaccgccaa attcacttac gattgcgcta
     6901 gctgttaatg ctgggcttag aggactattg tcagaaggat ataaaccgcc cgttgttaca
     6961 atgacgccat tgttaatggt taacttacta ccctctcctg caacggatac ccgtaacttc
     7021 cattacccgt tgttataact agcgcattat caatatcttt tacttttcca tcttttattg
     7081 ccacagcatg tttatattca tcgtgctgaa taaactgttc agcatatgca ttacccaccg
     7141 ccagagcaat aagtcccaaa ctaaacccgg ggagtttttt cagtttcata gattaactat
     7201 ccattcattg gattaacaaa cattcctggt caaataacct atgagctcag attaccaaaa
     7261 cggagggtaa agacaaaaac aaaagcacaa aaaatttaaa tagataaaca gcgacaatat
     7321 ataaatgata aataagtcgg cattatcatt tttcacctta ttttcatgac attgatcact
     7381 ttggggtgac tattttgttt aacaaataac tcttcgacat caaaattatt aatagttgcc
     7441 atggtaatta tttcggtgat atataaaaac aactgtgata acaccaaggt tgacaactat
     7501 ttgcgcaata attcctttcc ttcaatgatc caggcgttca ctgccagcaa cccattcagc
     7561 cgcatactga cagtgagaaa ctgaaagatt attgctgatc cctgtggcta acgagtcttt
     7621 ctcttaaatc gattaaccag attgatcgcc agccaaacaa gggcattaag caaatttatc
     7681 gtcacaagca cttctcccag cccggtactg ggttttgcaa taaggatgac caggagggtg
     7741 atcagcataa atgcaatgga aaagatgata tagagagtga aataatccag attattacta
     7801 ttattcatgc tactaatggc aatcctgtca aaactttcaa acaattatag caaacaaaat
     7861 aatgaacaat agtaacaaga ggcaacgagc taattagctg tctataatta caccgcacta
     7921 tggccttact gatcaatttt cactcaacca caaccttcac cttcggttta gaaaggataa
     7981 ttttgaccag gtcgatgtat gtaacaggtt tatagaaata ataaccctgc tgaaatgtga
     8041 tattattttg gttcagatag ccaagttgtt ctttcgtttc gacaccttca gcgacgatac
     8101 tgatcgaaag tttacgcgcc agatccaata cacaatccag aattcgggtt gattctgggt
     8161 ctgcgtttac gcggccaacg aaactgtgat caattttgat ataatcaata tgcaggtcat
     8221 gaagataaga aagccctgag tagccagtac cgaaatcatc cagcgcgatg acaaaaccat
     8281 tttcatgcag aatgttcaac cgctgaacca gactttcatc aacattcaat ggctcacgct
     8341 cggtgacttc cagaacaagg tttaaatcgc ggcgggtaaa actgtcacgg aaatttaaac
     8401 actcgtcgac aaacgtcggc gaaataatat gcgaagcgct aaaattaatt ccaatatgaa
     8461 aaccttccgg cagtttactc gcgatagcgt tcatctgtct ggcaacctga ttcatcaggc
     8521 tttgtgtaag cggaacgatt aatccggatt tttcagcaag tggaataaat gccgcgggtg
     8581 aaatatatcc accgtgaggt tgtttccagc gggctaacac ctcaactccc cgcaatgtcc
     8641 cttcccgacc atttaccaca ggttggtaaa aagggacgat ctccccatta tttattgccc
     8701 gtcgcaggat ctcttcaggc gttgcgcttt tattgaaata tctatcaagc aggaaggcag
     8761 cggcgctggc gattaacaga ataaatatta aaatcccaaa gcctttatcg ataagtctgt
     8821 agaagctaaa gagtggcggc gggttaaaga taatagtgaa atgatatttt gtggagtgaa
     8881 ccttctcggt atagggataa tgtccattaa gcgtcatcac atcagccgtt ggtccaataa
     8941 tgtcatccgc cacgcgtaat acatacctta cccctttcaa gggtacatta agcgccccgc
     9001 gaatatgttg atcacttatc gtaaccaaaa tgcgcgtatc tgcaaattgg ctctgataga
     9061 gcaggatagg taatctatta acggtgtcga ttgccggagc caacagtaaa ttactgtccg
     9121 ggacaacagg aatacgagac agcaggaccc gattcccagg cagggatgta caccagacta
     9181 ttccctgttt gagaatgatg attgtgcgca ggtgaggttt cagagctgct tcagtgccaa
     9241 gctgatattg ctcctcgagg tcgcattcct tcccggcaat gttaaccgct gtttgagtcg
     9301 cctgtgacgc ttcttcaagt atatgattga tattgttggc ggcatatctt gctcccgcca
     9361 ggtactctgc ttttgcggaa taccagagct ggatgttcaa aatagcgaca ccggtaataa
     9421 acaagcatat cgcaacgagg atgggtatga gtgtattgcg catttgcttg tcccggccaa
     9481 agggtttaat aca
//
LOCUS       DAETQL010000133         9413 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.134, whole genome shotgun sequence.
ACCESSION   DAETQL010000133 DAETQL010000000
VERSION     DAETQL010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.134"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            70..222
                     /gene="hokE"
                     /locus_tag="K0W65_003696"
     CDS             70..222
                     /gene="hokE"
                     /locus_tag="K0W65_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBI3096797.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            573..725
                     /gene="hokE"
                     /locus_tag="K0W65_003697"
     CDS             573..725
                     /gene="hokE"
                     /locus_tag="K0W65_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBI3096798.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(847..1467)
                     /gene="entD"
                     /locus_tag="K0W65_003698"
     CDS             complement(847..1467)
                     /gene="entD"
                     /locus_tag="K0W65_003698"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HBI3096799.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="K0W65_003699"
     CDS             complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="K0W65_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HBI3096800.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4125..5327
                     /gene="fes"
                     /locus_tag="K0W65_003700"
     CDS             4125..5327
                     /gene="fes"
                     /locus_tag="K0W65_003700"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HBI3096801.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5330..5548
                     /gene="ybdZ"
                     /locus_tag="K0W65_003701"
     CDS             5330..5548
                     /gene="ybdZ"
                     /locus_tag="K0W65_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HBI3096802.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5545..9390
                     /gene="entF"
                     /locus_tag="K0W65_003702"
     CDS             5545..9390
                     /gene="entF"
                     /locus_tag="K0W65_003702"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HBI3096803.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2049 a         2477 c         2598 g         2289 t
ORIGIN      
        1 cctggggctg ccactccaaa ccagacaatt tggatggtag tcccttcttc gcatggaggt
       61 aatataaaca tgctgacgaa atatgccctt gtggcagtca tagtgctgtg tttgacggta
      121 ctgggattta cgcttctggc cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat
      181 attgagttca gggctgttct cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa
      241 tccccgcata tccggttgtc aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg
      301 cggtcagtga tggctgcggg cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga
      361 agtccgaaac aggactcact gtataaataa acagctattt tgctaaggaa gggtaagata
      421 acggcgggtg cctgaagctt tccggtttca ggttaactct gaggtttgga aagatgaagc
      481 cccaagagat atttctatca atcctggggc tgccactccg aacctcaaca actcggatgg
      541 tagtcccttc ttcgcatgga ggcaatataa acatgctgac gaaatatgcc cttgtggcag
      601 tcatagtgct gtgtttgacg gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg
      661 agttcaccgt aaaggaacgt aatattgagt ttaaggctgt tcttgcttac gaaccgaaga
      721 agtagccgtt gtgcggggag taatccccgc atatccggtt gtcaggtcag agtggtaagg
      781 cacctgcttt acactttcgc ccgcagtcag tgatggctgc gggcgaatcg taccagatgt
      841 tgtcaattaa tcgtgttggc acagagttat gactattttt tcttttatct gccagtgcac
      901 agcaaacatc tcattctcac gatgaatgat gacctgctgt ttattccagc taattatctg
      961 atagtccaga aaacctgcat cagtttggat ctcacttgcc ttaaatgcgc tctctttggc
     1021 ggaaaatgca agtgtcagcg ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg
     1081 ttctgctggt gtaataatgt tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc
     1141 ttcgatatca acgccaatcg gttgacggga aaccacggcc aatgccgtag ccccgcagtg
     1201 gctaatactg ccgtatacct ccgcaggcca caccggttgg cgtagctcgc cgattgcggg
     1261 cacacattta tagccatatt cccgcaaggc ataaacagca gcgatccgtc cggctaaatg
     1321 ctctgtttta cgtttacgtc cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa
     1381 atcctgctca caaaaattcg ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa
     1441 ggggagggag gtatgcgtag ttttcatatc gaccatattc gagactgatg acaaacgcaa
     1501 aactgcctga tgcgctacgc ttatcaggcc tacgtgatcc tgcaatatat tgaattggca
     1561 agatctttgt aggccggata aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt
     1621 gtcaacaatc tgacgttagc atcagaagtg agtattaatg ctcatatacc acgtacgtcc
     1681 cggctcgtta taggtatacg cgccggcacc ggcgatatag ttggctcctg ccagatcgcc
     1741 cgtggtctgg gcattacctg cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc
     1801 ggtcagactg acattcttcg tcacatccca ggtcgcgctc agacccacaa tgctgtaagg
     1861 actgatttct ttggtttccg ggccaaccgc tggctgacct ttatagttgt acttcttcgg
     1921 ctgctgcttg ccgtaccagg tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca
     1981 gctcagcgtt gagttcaacg tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt
     2041 gttttcactc ttcagcatat aagtgatgtt attggtccac atcaccgttt cgctaactgg
     2101 tacgtttaac gatccttcca gaccttcaac cactgctttc ggcacgttat cccactgata
     2161 gagatcagtg ccgactgcgt tttgccctac agccacatag cccgcttcaa tcttattgcg
     2221 ataatcgtta cggaaccagg tgacgcccgc cagccagccg tcgcgtttga actccagacc
     2281 aatctctttg ttgatgctgg tttctacttt caggtcatcg ttaccttgca gatagcagcc
     2341 gcccgcgctg gcatagcagc cctgaccttt actgtagaga atgtagttcg ggttagtctg
     2401 gtataggctc ggcgctttat aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc
     2461 taaaccttgc gatatgttca gcgccgggct ccagttattg ccgacaatac tgtgatgatc
     2521 gaaacgcagc cccggcgtta cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa
     2581 cagcgagaaa atttctgctt ttgaatacgg gctacggtcg gtggcactca cgccatcaat
     2641 agcgccaccg gtattcgttc cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg
     2701 ttgctgattc cactccgtac ccagcgtcag cgtctggtta acgaggaaat caatcggcag
     2761 gttaacttcg ctgtgcagca tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc
     2821 tttttcgtta aatttccctt cggtaccgcc cgccagacct tccggaatac gcgagttacg
     2881 ggtgtgttca tactgcaccc agttgctggt ggtcacgccg ttatcccagc caccgttcca
     2941 ggtcagcgcg tagttctggc gatacaggcg gttggtttca tcgccatatt tcgagcgagt
     3001 gtaagcgtca gagttggtgt tctgggtatc gcccgcatac aggttaccct ggcggctgta
     3061 acccgcttcc agttccagcg attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat
     3121 atctttattg atcacccctt cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg
     3181 cgcggactga tggccctggt tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc
     3241 atacaagcgg aagctgaatt cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt
     3301 ggtggcacct tcctctttat gttctggcgc attgaaatat gcgtcccagg agccgtgcca
     3361 ctcgccgctg ccttttttgg taatgatgtt aaccacgccg cccgccgcgc cgttgccata
     3421 acgcgcagct gccggaccac gcagaacttc aatacgttca atcatttcag gtggcaccca
     3481 ggaggtatca ccacgggtat cgcgctcgcc acgccagccc tgacgcaccg agttacggct
     3541 gcttaccggc ttgccgtcaa tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc
     3601 aatctgtcgg ttattcccac gctgaccact ggtggagtta ccggtcaggt taacgcctgg
     3661 catggtacgg atgatctccg acacatcgcg ggcaaccggg tttttgcgga tttcatctgc
     3721 ggtgatggtc gaaacgccag gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt
     3781 atcgtcatgt gaaacaggtg tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc
     3841 cagattgacc aacaaggcca gggaatgaat cttcttgttc attgttttat tcctgcattt
     3901 ttgccacgaa ttgcaactgt cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc
     3961 gaaaaccttt gatcctgaaa gacacgcagt gcagttggtt aattaatgtc cgcgcttccc
     4021 acggcgcgcc attacgctat tgcaaatgca aatagttatt aataacatta tcaatatatt
     4081 tctgcaatca atgaaaaatt gcacagtaaa catggggtta tggtgtgacg gcgttaaaag
     4141 taggaagtga gagctggtgg cagtcgaaac atggcccgga atggcagcgt ctgaatgacg
     4201 aaatgtttga ggtcactttc tggtggcgtg atccccaagg ttctgaagaa tactcgacga
     4261 taaagcgcgt atgggtctac atcactggtg tgaccgatca tcatcagaac agccagcccc
     4321 agtcgatgca gcgaattgca ggtactgatg tctggcagtg gacgacacaa ctcaatgcca
     4381 actggcgcgg cagctactgc tttattccca ccgaacgcga tgacattttt tctgcaccat
     4441 cccccgatcg cctcgaatta cgtgaaggct ggcgaaaact attaccccag gcaatagccg
     4501 atccgctgaa tccacaaagc tggaaaggcg ggctagggca cgctgtttct gcactcgaaa
     4561 tgccgcaagc gcctctgcaa ccgggatggg attgtccgca agcgccagaa ataccagcca
     4621 aagaaattat ctggaaaagt gaacggttga aaaattcacg tcgtgtatgg atttttacca
     4681 ccggcgatgt aacagcagaa gaacgcccgc tggcagtttt gctcgatggc gaattttggg
     4741 cgcaaagtat gcccgtctgg cctgtgctga cttcgctgac ccatcgtcag caacttcctc
     4801 ccgccgtgta tgtgttgatc gacgctatcg acaccacgca ccgcgcccac gaactgccgt
     4861 gtaatgcgga tttctggctg gcagtacagc aagagttatt acccctggtg aaagttattg
     4921 ccccttttag cgatcgcgcc gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt
     4981 ccgcgctgta tgccggactg cactggcctg aacgctttgg ctgtgtatta agccagtcag
     5041 gatcgtactg gtggccgcat cggggcgggc agcaagaggg cgtgttactt gaaaagctaa
     5101 aagctggtga agttagcgcc gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc
     5161 cgatgatcat gcgggccaat caggcgctgt atgcgcaatt acaccctata aaagaatcca
     5221 ttttctggcg tcaggttgac ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc
     5281 aggggttaat cgacctctgg caaccacttt tccatgacag gagttgaata tggcattcag
     5341 taatcccttc gatgatccac agggagcgtt ttacattttg cgcaatgcgc aggggcaatt
     5401 cagtctgtgg ccgcaacaat gcgccttacc ggcaggctgg gacattgtgt gtcagccgca
     5461 gtcacaagag tcctgccagc agtggcttga agcccactgg cgtactctga caccggcgaa
     5521 tttcacccag ttgcaggagg cacaatgagc cagcatttac ctttggtcgc cgcacagccc
     5581 ggcatctgga tggcagaaaa actgtcggat ttaccctccg cctggagcgt ggcgcattac
     5641 gttgagttaa ccggagaggt tgatgcgcca ttactggccc gcgcggtggt tgccggatta
     5701 gcgcaagcag atactttgcg gatgcgtttt acggaagata acggcgaagt ctggcaatgg
     5761 gtcgatgatg cgctgatatt cgaactgcca gaaattatcg acctgcgaac caatattgat
     5821 ccgcacggta ctgcgcaggc attaatgctg gcggatttgc aacaagattt gcgcgtcgat
     5881 agcggtaaac cactggtctt tcatcaactg atacaggtgg cagataaccg ctggtactgg
     5941 tatcagcgtt atcaccattt actggtcgat ggcttcagtt ttccggccat tacccgccag
     6001 atcgccaaca tttactgcgc attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg
     6061 cctttcgctg atgtagtgga agagtaccag caataccgcg aaagcgaagc ctggcagcgt
     6121 gatgcggcat tctgggtgga acagcgtcgc caactgccac cgcccgcgtc actttctccg
     6181 gcacctttag cggggcgcag cgcctcggca gatattttgc gtctgaaaat ggaatttacc
     6241 gacggggaat tccgccagct ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc
     6301 cttgcgctga cggctttgtg gctggggcga ttgtgcaatc gcatggacta cgccgccgga
     6361 tttatcttta tgcgtcgact gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac
     6421 gttttgccgc tgggtattca tattgcggcg caagaaacgc tgccggaact ggcaacccga
     6481 ctggcaaccc gactggcagc acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc
     6541 gaacaaattg tccgtgacag cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta
     6601 ctcaatatta aggtatttga ttaccaactg gatattcctg gtgttcaggc gcaaacccat
     6661 accctggcaa ccggtccggt taatgacctt gaactggccc tgttcccgga tgaacacggt
     6721 gatttgagta ttgagatcct cgccaataaa cagcgttacg atgagccaac gttaatccag
     6781 catgctgaac gcctgaaaat gctgattgcc cagttcgccg cggatccggc gctgttgtgc
     6841 ggcgatgtcg atattatgct gccaggtgag tatgcgcagc tggcgcagat caacgccact
     6901 caggttgaga ttccagaaac cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca
     6961 ccggatgctc cggcgctggc agatgcgcgt tacctgttca gctatcggga aatgcacgag
     7021 caggtggtgg cgctggcgaa tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg
     7081 gcggtggcac taccgcgctc ggtctttttg accctggcac tccatgcaat agttgaagct
     7141 ggcgcggcct ggctaccgct ggataccggc tatccggacg atcgcctgaa aatgatgctg
     7201 gaagatgcgc gtccgtcgct gttaattacc accgacgatc aactgccgcg ctttagcgat
     7261 gttcccaatt taaccaacct ttgctataac gccccgctta ccccccaggg cagtgcgccg
     7321 ctgcaacttt cacaaccgca ccacacggct tatatcatct ttacctctgg ctccaccggc
     7381 aggccgaaag gggtaatggt cgggcagacg gctatcgtca accgcctgtt gtggattcaa
     7441 aatcattatc cgctcacagg cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat
     7501 gtctcggtgt gggagttttt ctggccgttt attgccgggg ctaaactggt gatggctgaa
     7561 ccggaagcgc accgcgaccc gctcgctatg cagcaattct ttgccgaata tggcgtaacg
     7621 accacgcact ttgtgccgtc gatgctggca gcatttgttg cctcgctgac tccgcaaacc
     7681 gctcgccaga gttgcgtgac gttgaaacag gttttctgta gtggtgaggc cttaccggct
     7741 gatttatgcc gcgaatggca acagttaacc ggcgcgccgt tgcataacct gtatggcccg
     7801 acggaagcgg cggtagatgt cagctggtat ccggcttttg gcgaggaact ggcacaggtg
     7861 cgcggcagca gtgtgccgat tggttatccg gtatggaata cgggtctgcg tattcttgat
     7921 gcgatgatgc atccggtgcc gccgggtgtg gcgggtgatc tctatctcac cggtattcaa
     7981 ctggcgcagg ggtatcttgg acgacccgat ctgaccgcca gccgctttat tgccgatcct
     8041 tttgctcctg gtgaacggat gtatcgtacc ggagacgttg cccgctggct ggataacggc
     8101 gcggtggagt acctcgggcg cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa
     8161 ctgggcgaaa tcgatcgcgt gatgcaggcg ctgccggatg tcaaacaagc cgttacccac
     8221 gcctgtgtga ttaaccaggc ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat
     8281 ctggtatcgc aatcgggcct gccgttggat accagcgcat tgcaggcgca gcttcgcgaa
     8341 acattgccgc cgcatatggt gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc
     8401 gccaacggca agctggatcg caaagcctta ccgttgcctg aactgaaggc acaagcgcca
     8461 gggcgtgcgc cgaaagcggg cagtgaaacg attatcgccg cggcattcgc gtcgttgctg
     8521 ggttgtgacg tgcaggatgc cgatgctgat ttctttgcgc tgggcggtca ttcgctactg
     8581 gcaatgaaac tggcagcgca gttaagtcga cagtttgctc gtcaggtgac gccggggcag
     8641 gtgatggtcg cgtcaaccgt cgccaaactg gcaacgatta ttgatggtga agaggacagc
     8701 tcccggcgca tgggattcga aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg
     8761 ttttgtttcc atccggcgtc cggttttgcc tggcagttta gcgtgctctc gcgttatctc
     8821 gatccacaat ggtcgattat tggcattcag tcgccgcgcc ctcatggacc catgcagaca
     8881 gcgacaaacc tggatgaagt ctgcgaagcg catctggcaa cgctacttga gcaacaaccg
     8941 cacggcattg cggcgcggct gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg
     9001 gatacctggc cgccagagac gcaaaactgg caggaaaaag aggctaatgg tctggacccg
     9061 gaagtgctgg cggagattaa ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt
     9121 acttcaacgg agttgtttac caccattgaa ggcaactacg ctgatgctgt gcgcctgctg
     9181 acgactgctc atagcgtacc gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg
     9241 cttcaggaag gtatgagccc cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc
     9301 tatcgtcagg attgtgcgca tgtggatatt atctctccag gggcgtttgt aaaaattggg
     9361 ccgattattc gcgcaacgct aaacaggtaa attaatatta tctataaacc cat
//
LOCUS       DAETQL010000134         9328 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.135, whole genome shotgun sequence.
ACCESSION   DAETQL010000134 DAETQL010000000
VERSION     DAETQL010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.135"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="K0W65_003703"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="K0W65_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBI3096804.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="K0W65_003704"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="K0W65_003704"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBI3096805.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="K0W65_003705"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="K0W65_003705"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBI3096806.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="K0W65_003706"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="K0W65_003706"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBI3096807.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="K0W65_003707"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="K0W65_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBI3096808.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /locus_tag="K0W65_003708"
     CDS             5562..6035
                     /locus_tag="K0W65_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBI3096809.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="K0W65_003709"
     CDS             6064..6606
                     /locus_tag="K0W65_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HBI3096810.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="K0W65_003710"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="K0W65_003710"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBI3096811.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="K0W65_003711"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="K0W65_003711"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBI3096812.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="K0W65_003712"
     CDS             8003..8260
                     /locus_tag="K0W65_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBI3096813.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="K0W65_003713"
     CDS             complement(8236..8790)
                     /locus_tag="K0W65_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBI3096814.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            9261..>9328
                     /locus_tag="K0W65_003714"
     rRNA            9261..>9328
                     /locus_tag="K0W65_003714"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         2358 a         2254 c         2318 g         2398 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagaga aaaaatcctg aaattcaggg ttgactctga
     9061 aagaggaaag cgtaatatac gccacctcgc gacagtgcgc taaagcgcgt cgcaactgct
     9121 ctttaacaat ttatcagaca atctgtgtgg gcactcgaag atacggattc ttaacgtcgc
     9181 aagacgaaaa atgaatacca agtctcaaga gtgaacacgt aattcattac gaagtttaat
     9241 tctttgagca tcaaactttt aaattgaaga gtttgatcat ggctcagatt gaacgctggc
     9301 ggcaggccta acacatgcaa gtcgaacg
//
LOCUS       DAETQL010000135         9328 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.136, whole genome shotgun sequence.
ACCESSION   DAETQL010000135 DAETQL010000000
VERSION     DAETQL010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.136"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(139..1041)
                     /locus_tag="K0W65_003715"
                     /pseudo
     CDS             complement(139..1041)
                     /locus_tag="K0W65_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1101..1379
                     /locus_tag="K0W65_003716"
     CDS             1101..1379
                     /locus_tag="K0W65_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096815.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="K0W65_003717"
     CDS             complement(1380..1907)
                     /gene="gadE"
                     /locus_tag="K0W65_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBI3096816.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="K0W65_003718"
     CDS             complement(2706..3278)
                     /gene="hdeD"
                     /locus_tag="K0W65_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBI3096817.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3533..3865
                     /gene="hdeA"
                     /locus_tag="K0W65_003719"
     CDS             3533..3865
                     /gene="hdeA"
                     /locus_tag="K0W65_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HBI3096818.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3981..4307
                     /gene="hdeB"
                     /locus_tag="K0W65_003720"
     CDS             3981..4307
                     /gene="hdeB"
                     /locus_tag="K0W65_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBI3096819.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4371..5017
                     /locus_tag="K0W65_003721"
                     /pseudo
     CDS             4371..5017
                     /locus_tag="K0W65_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(5059..5589)
                     /locus_tag="K0W65_003722"
     CDS             complement(5059..5589)
                     /locus_tag="K0W65_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3096820.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5745..6311)
                     /gene="slp"
                     /locus_tag="K0W65_003723"
     CDS             complement(5745..6311)
                     /gene="slp"
                     /locus_tag="K0W65_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HBI3096821.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6558..7781)
                     /locus_tag="K0W65_003724"
     CDS             complement(6558..7781)
                     /locus_tag="K0W65_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096822.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8362..>9328)
                     /locus_tag="K0W65_003725"
     CDS             complement(8362..>9328)
                     /locus_tag="K0W65_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBI3096823.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2719 a         1857 c         1940 g         2812 t
ORIGIN      
        1 tttatcgata tcgaagcatt gagcattttt aactcgtttt atcttgaatg caatcggcgc
       61 ataacttatt gaatcccatc aaatcccgtc tttttttacc cattcccggt tatcttgaat
      121 aaattaccta gtcataaata cattctggcg gctaccttca tccactaatg ccgtgacata
      181 catgccaggc agcaagtcac catttgggtt ggggaaaatc gcccgtaacg tcacggagcc
      241 cgtggtttca tcaaccgtcg ggtcggagaa tttcagcgtg ccggtctggc tgtaacgttt
      301 accattttcc agattgagct gtactggcgt actgccctga acctgtttga tttgcccact
      361 ggcgacctct tctttcatgc gtaagaaatc ttgcaccgac tgcgtgagat cgacataaat
      421 cgggtccaga cgttgtacgg taaccagcga atctgcctga ttagcggtaa cgagtgcgcc
      481 gacggtcacc gacgatttcc cgctgacgcc cgtaatcggc gaggtgacat tcgcgtattg
      541 cagattgatc gtcgcctgtt caacagccgc tttggcgacg gtgacattgg cttctgcttc
      601 attcagctgg gtgcgcgcgg tgtcgtaatc ctgacggcta acgtagttgg tcttcagcaa
      661 cgatgcctgg cggttaaagg tgatgcgggc attgctggcg gtagagagcg ctttcgccag
      721 cgagcctttg gcggagttta gctcggcctg taaaggtgca ggatcaatct gatacagcga
      781 atcgccctgg ttcactttat cgccttcgat aaagttgcgt ttaatgataa taccgcccac
      841 ctggggacgt atctcggcaa cttcataagg aacggttcta ccgggcaatt cgctcaacac
      901 attgaccgaa ccaggggaga gtgtgacgac accgacctca ggcgtcatgg cggcggcgtt
      961 ttccgccgat ttgtcatcgc aggcggtgag catcgcgccg cagaataaca acggtattaa
     1021 cagctttctt cttctgttca ttttagtccc tgaaaattct tgagaaataa cggttcttta
     1081 aattgcgcgc gaggagagat atgagggatt tacagaccag cggcatagtg gggttgtcag
     1141 ccagtaaggt gggatacagg cacagtgatc gacatggtga ggtcaacgac aaaaaaaatg
     1201 ttgccgttct gccaacagtt cctgccagca ttcgggcaac gaagggttct actggtggat
     1261 acacatacca ggggaatcaa gtcgttttag cctctccttt agtaatcacc gggggtaatg
     1321 aaatcagcat ctgcatccct cgtcatgcca gccatcaatt tcagttgctt atgtcctgac
     1381 taaaaataag atgtgatacc cagggtgacg atgtcgctca tacgtcttag tttcagcttg
     1441 tacataatgt tgaatttctg aatctttaat gtttgttgcg aaatattgag cgtcgacgtg
     1501 atattgcttt catttttatt tttcagaatc agttgcataa ttttgaactg attatgtgaa
     1561 aaacataatt gtgaattctt atggggcaag tgtttaccat aagtaatctc aagaagattt
     1621 ttgatacttt ctttgcggct aacaaaataa ataggcgctt tagcttttag tttactgatg
     1681 tgataaggtg ccaaaacaat aattctctcg gcatctaatt tctccagaaa tttaatcgct
     1741 tcttcatcaa ggatatgatt gtgataggta tcaatgataa ccttgaaggg tttattgcct
     1801 acttttttga tctctgacag ggaattgatt tttatcattt cgtgattatc tttcaactgc
     1861 caaaagccct gtaaaagaaa agaatctttc gtcatgagaa aaatcataac ttgcgcctta
     1921 gccgttatcg tttcgaacat gtcatccttt tcattattta catccttgtc cgaatcgttg
     1981 ttcaatatag taaacgccta taagaacttt gcccttcgtg aactaatacg tctattaaca
     2041 ctaatcaata cttcagattt atccatcacg cactccatat attgatccta agtaagattc
     2101 ctggttgtta tcagcttgta aatagtaaac aaaaagttaa cgtttgcgga ttactgccca
     2161 agaataagtt atcaataacc agtttgtgat ctctgaagaa tattactaaa gttaaaatgt
     2221 aatccgattt aaatatcgag tctccttgtt tcgacttaag ctggcaattg gattgccagc
     2281 tttcttttaa cgcattgaaa ttacctgagt aaataacgca ttcatgtctt caggttttta
     2341 tatagcgtct ggagacacgg atatttatgc aatgtaatca acgccagttg caaaaaaatg
     2401 aacccggatt acttatttaa ccgcccatag catcaacgct tacggaactc tttgttcact
     2461 ttcccacagc acttttaaaa cattaaacct gactacggaa aatatcagcc atgataatgt
     2521 ttgaaatggc taatttgcca tggagtgaaa aaaattagat gaaattcagt aggttgaaat
     2581 aatcactagc aggtaattat ttcaatgata gtgcgcaatt gatctacaac actgcgtagc
     2641 ggagagagta ttaatcggat catagtcaca tcaagtgact atgatccggg tgacaaccgg
     2701 ggtaattatt gctgcttaac gaacaaactg gcgaagctga acaggctggc ggcgctaaat
     2761 atcagttcaa ttcccaccag tgtggaaacc agcgttacag acaccatcgg cgttgcacca
     2821 aggaatatcc aggcaatgac gatatccagc acaccaataa cgagctgtag ccagctgcct
     2881 ttcattgaac gctgacgata ccaactcatc aggcgaataa cccctgcaac acagaacaaa
     2941 ccggcaataa atgccgcaat ggcaaaaatg cccagctccg gtgcacggat gaagaaatag
     3001 ccgatcaata aataggcgac tgcgacgagg aaaccggata ataccggcca gaaattatga
     3061 ctgcggttgc tgaataaccc gacaataagc gcaatacccg agcagattaa taatgcaccc
     3121 actactgtgc ttaaaatatc gccagagacg aacgggaaac tgatacacag caacccgacg
     3181 ataaacagca gcacggcaat aaactggatt gctctgcgat gctttttcag catctccaga
     3241 tcaaacttca aaattgttgc cttatctata tataacatag aaccacccta taaaattaag
     3301 aagaaaatcc cctgctatca atctatgcca aaaacgcgtc taagaatgca gtcgatttaa
     3361 taaaaatttc ctaattgcag tatctgatgc atctgtaact cattgtattg aaataaaaat
     3421 atctgatttt gatattttcc atcaacatga catatacaga aaaccaggtt ataacctcag
     3481 tgtcgaaatt gattcgtgac ggctctttca ctttatagtt gaggatatta cgatgaaaaa
     3541 agtattaggc gttattcttg gtggtctgct tcttctgcca gttgtgagca atgcagcgga
     3601 tgcgcaaaaa gcagctgata acaaaaaacc ggtcaactca tggacctgtg aagatttcct
     3661 ggctgtggac gaatccttcc agccaactgc agttggtttt gctgaagcgc tgaacaacaa
     3721 agataaacca gaagatgcgg ttttagatgt tcagggtatt gcaaccgtaa ccccagctat
     3781 cgttcaggct tgtactcagg ataaacaagc caactttaaa gataaagtta aaggcgaatg
     3841 ggacaaaatt aagaaagata tgtaattccg ggaatgcgtt acatcgtact tccttgcata
     3901 ttgaacaggc cggaatatct tctttaaaag cagctattcc tcctgttcat atataatctc
     3961 tatattgaat gggttacaaa atgaatattt catctctccg taaagcgttt atttttatgg
     4021 gcgctgtagc ggctttgtca ctggtgaacg cacaatctgc gttggcagcc aatgaatctg
     4081 ctaaagatat gacctgccag gaatttattg atctgaatcc aaaagcaatg accccggttg
     4141 catggtggat gctgcatgaa gaaacagtat ataaaggtgg cgataccgtt actttaaatg
     4201 aaaccgatct cactcaaatt cctaaagtga tcgaatactg taagaaaaac ccgcagaaaa
     4261 atttgtatac cttcaaaaat caagcatcta atgacttgcc gaattaatga ggtacaagta
     4321 aaaaggagta gcaagttgag ccatcttgct gctccttttt gcatttttat atgacagcag
     4381 aatttattat acgtcttata ctcgcggcaa ttgcctgtgg cgctattggc atggaaaggc
     4441 aaatgcgcgg caaaggagca gggttacgca cacatgtatt aattggcatg ggaagcgccc
     4501 tgtttatgat tgtttcgaaa tatggttttg ctgacgtgct gtctttagat cacgtcggac
     4561 tcgaccccag ccgtattgct gctcaggtgg tgacgggcgt cgggtttatc ggtgcaggta
     4621 acattctcgt tcgtaaccaa aatattgtag gtctgacgac ggcagcggat atctgggtga
     4681 ccgccgcata ggtatggtta ttggcagcgg tatgtacgaa ctgggtattt atggttcagt
     4741 gatgaccttg ttggtgctgg aagtcttcca tcaattaacc ttccgcctga tgaacaaaaa
     4801 ttaccattta cagctgacat tagtgaacgg taatacggta tcaatgctcg actggtttaa
     4861 gcagcaaaaa ataaaaactg atttagtttc gttgcaggaa aatgaagatc atgaagtggt
     4921 cgccattgat atccagcttc atgccacaac gttgatagaa gacctgttgc gattattaaa
     4981 aggtatggca ggggtgaaag gcgtttcaat tagctaatct gtgtggcagc gtagccagac
     5041 tcaccgtaag cctgaaattc acaccagata atcaatatgc tgatggcgta ccagctcatt
     5101 gatccgctta agattgagga tcatcatgat attgtgttta tggcagtaga acgtttttag
     5161 cgatattttc agtttctcca gaatatcttc agttgtcatt tgattcgcaa tgcagctcag
     5221 aacctgatgt tgcttgcgcg aaagtgacac attcgcttca ggcacagagc agtaactgcc
     5281 attgatgagt gcgacaaaat caatgatagt gatagtccgc ggaacaaagg tcaccggtac
     5341 tttccccaga cagcgtttaa tattaaaggg ggagaaaaca atgacatgga caggttttaa
     5401 tcgttcaatt tgagtgagaa aattagaatg aaatacatta ttcattaatg tatcaataat
     5461 gacgaaggca ttctgatgca acattacgtc gatctcttct tcgttctgta tatgaacgac
     5521 attaccttta ctcagaatgt ttttcatcgc ggtgaagaac atcgtatccc tggtaattat
     5581 aagaaacata accgactcct ggtgcggaca ttaactttat aatcagtttg ccaaaggtaa
     5641 ttaacaggac agggcttata ataataatag ttagcttact gtatattaat tttatgtaat
     5701 acatgatgca gatgctgaga aagcatctgc atctttcggt ggtgttattt gaccagctca
     5761 ggtgttacct gactcaccgc attggtgtag taaggcgcac cccagcccgg ttccggccag
     5821 aatgcaccat agccgtaatc ccacaggtta taggtggtat taaccacttc tctcaaatgc
     5881 cacacctgga tgccctgcat attcacttcc aggaagttat acgggacttt attgataaag
     5941 cctggttgtt caccctgaat ggtaccgagg atggtaacaa agtgattacg atagttcact
     6001 ggatcaagga agccgctttg tctggcgagc agtcggccct gatagttggc ttcaatatca
     6061 ggcttcgcat agctatccaa cggtaataca gcgatttcta acaacgtatc cgttttgcca
     6121 ttgataacgt tgataacctt cccaccaaag cgcgcttgtt gaccaacata taaccccggc
     6181 tggttatgaa cagcaacaaa acttttttga atatcaggtt ggttattgcc tttgatattt
     6241 tgcggaattg aactacatgc ggcaagcaaa aatgaaaggc tgaggatgag tgcacctttt
     6301 gtcatgttca tgttactatc cttatcaaca aatattaaaa ccataataga tatttcttta
     6361 tagttttcat ctgattctga ggtgatatgc ctgatagtaa cgtaaacctg tctgatgttt
     6421 atatcccttc tgtttagttc aattccgtta ttttggggcg tggtatgagt atccatcgcc
     6481 caataatata accctttgtt ttaaaattac ataaaatcta tttttattca atgattataa
     6541 agtgctaata atcaatatta tggtattata ttctgataat gatttgataa gcgaattctt
     6601 ttgccagatg gtatttctat ttccacccca taatttatat ctttttcatt aggcagatct
     6661 ctctttaaat aaaaggggta tgaaatcttt tcgggttgaa gtgtctcgta atgaaattct
     6721 atgaattcat ttttagacat tgttgcccat ttttgaattt tattgtatag agagggtgtt
     6781 gtaaactctt gaagttgttt ataacatatt tttttgaact cttgcttata ttttaatatg
     6841 tcatcaaatg aaagattcac tacctttgcc tctaagaaat caattattct cgaagcaatt
     6901 tccgctttcg cgcgtaaaga gttgtcatta atagttaaca atgtagaaaa caaaccattg
     6961 atttgtttgt caagatgttg ttcatattta tgtagtttgg ggatgggatt ttccttgtgt
     7021 gaaaaaatag tgttaaataa atttgcgtca gtgaaattta tattaagtag atcttcaaaa
     7081 taaaattttg ataaggttag gactagcttg gacatatctt gccctgtcat gttgaaatca
     7141 ataaagtcgc atccagcaag ggcttccatc ctattttcat ttttactgtg ctcaatatac
     7201 agcttcgcca tttttaagta caagctttga atgtctctta tatcagttgg taagtcagct
     7261 cgttgcccag caaaattttt attatcggaa gaaacggttt ggacactgaa tctttcgggt
     7321 attccttctt ctacgagaat tttagttatg attggattgt tgggttctgg attaacgaca
     7381 acaatgattt gacatacaga atccttatcc ttttcgtatc taatgttatg aatctgatta
     7441 agtattgatt gtgtctgttg cgtaagtggt gtcgaactta tcgtttcaat gttattaata
     7501 agacttacaa gttgaaccac aacttttgag tagtctgcat ttgtatatgt ggtgttcgtg
     7561 tggcatgcat taatttgttg gagttgttgt tgcgctaaat caaattgttc agggttgtat
     7621 ccttctccag agaatgtgga attaatgggt tgttgttctt cccaattatc acaaggccac
     7681 ttcggtgtgt ctggcgtttt aacattatcg gtatttgttg tgcagttata aatatcgccg
     7741 tgatatgtat ttattatacc tggggtaaag tcaattgaca taacattcct tattcatcat
     7801 ctgttttatg ctaatgtttt taaattggat aattagaaca ggaatggatt ttcttctaca
     7861 cacaataaat ataatgcgtt atatcttcct gatataatta cctttaaatt ccctttcatt
     7921 tccacccggt aatatttgtg tataaaaaaa gggcagtgat agtatcgtaa aggaaaacgt
     7981 taacaactat caaaaaacgc tcattttaaa aagttattat gttctccgat agtgtgtgaa
     8041 gtaatatttt tgtgatgttt tagatgtgta ataatgaacc aggttaaagt gtatttaaaa
     8101 atgtgttgta ttagcgactg aaatctgcct tttaaaaagg aggagggtac tgagacaaag
     8161 agtcattgct tctccgataa aatgtcagag aagcatgatt acgtggttat tgtctgttga
     8221 tgacttgttg accgagccgt catcattctc aatgcattag ttaaatcatt tgtgattttt
     8281 aaggattatc ctgtctatga ttttctactt agaaggatat ggcactaact atgcatctta
     8341 tagtttactg ctggcatcta tttaaaatag caattgagtc aaatagccca tcaagatagc
     8401 catacctaat ataaccgtca ggaatgcagc tatcatcggc attctgaaca ttgatttaag
     8461 taatatcact tccgtcaggc ttgcgccggc actgccgatg attaatgcca ttaatgctcc
     8521 cagacccatt ccttttgtca tcagaacaga tgccagaggg ataaccgcct cagcccggat
     8581 atacagcgga ataccgacaa cggcgcttaa tgggatggcg aagggattat ctgctcctgc
     8641 atgagcggca atccacgctg agggaataaa gccataaata aaagatccta ttaatactcc
     8701 taacaaaaga tatggcagta catctttaaa ctgttgcaac gcatctttgg tggctagttt
     8761 tatgggatta atacttacta cagctttggt actgcaacag gtaactacgg ggttctcaat
     8821 ggctttagcc gctggactac aacaactcac ttttatgggc gtatatgtcg ttcccggcga
     8881 agtttttgct ggagcacaac aatttgctga tgagctttta ctggcaatga tatgacgttc
     8941 aaaccccaga gaatccagga taatactggc aagaacggag acgccggctg cgataatcgc
     9001 gtacaacaag gtgactttcc aaccaaaggt catccacatt agtccgacga taatgggatt
     9061 aagtaatggt gaaacaaata aaaaagtgag agtcggacca aacccggctt tcgctgacaa
     9121 caatccacgt aacatgggta ttgtcgagca actacagaac ggggtaacgg ctcccaacag
     9181 agcagccagg agatagcctc tcccttttct ggcccccatc atctgctgga ttttatggtc
     9241 tggcaccttt tgtcttatca ggctgacacc ggcactaatc actataaaca gcaacgaaag
     9301 ctcgacggcg agaaacacaa acatctct
//
LOCUS       DAETQL010000136         9295 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.137, whole genome shotgun sequence.
ACCESSION   DAETQL010000136 DAETQL010000000
VERSION     DAETQL010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.137"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="K0W65_003726"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="K0W65_003726"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBI3096824.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..3047
                     /locus_tag="K0W65_003727"
     CDS             1647..3047
                     /locus_tag="K0W65_003727"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBI3096825.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHK
                     FGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRL
                     GREGYTKVQNASYQVAAYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTL
                     YDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSD
                     HPKLQGIAQQNSFKHT"
     gene            3417..4241
                     /gene="gadX"
                     /locus_tag="K0W65_003728"
     CDS             3417..4241
                     /gene="gadX"
                     /locus_tag="K0W65_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HBI3096826.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            4609..5337
                     /gene="gadW"
                     /locus_tag="K0W65_003729"
     CDS             4609..5337
                     /gene="gadW"
                     /locus_tag="K0W65_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBI3096827.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(5700..8813)
                     /gene="mdtF"
                     /locus_tag="K0W65_003730"
                     /pseudo
     CDS             complement(5700..8813)
                     /gene="mdtF"
                     /locus_tag="K0W65_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(8838..9095)
                     /locus_tag="K0W65_003731"
                     /pseudo
     CDS             complement(8838..9095)
                     /locus_tag="K0W65_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         2432 a         2163 c         2279 g         2421 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg
     1981 cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc
     2041 tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaaaccaa
     2101 cggataaacc aaacctagtg tgcggtccgg tacaaatctg ctggcataaa ttcgcccgct
     2161 actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt atggacccga
     2221 aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga
     2281 cctacaccgg taactatgag ttcccacaac cgctgcacga tgcgctggat aaattccagg
     2341 ccgacaccgg tatcgacatc gacatgcaca tcgacgctgc cagcggtggc ttcctggcac
     2401 cgttcgtcgc cccggatatc gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg
     2461 cttcaggcca taaattcggt ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg
     2521 aagaagcgct gccgcaggaa ctggtgttca acgttgacta cctgggtggt caaattggta
     2581 cttttgccat caacttctcc cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc
     2641 tgcgcctcgg tcgtgaaggc tataccaaag tacagaacgc ctcttaccag gttgccgctt
     2701 atctggcgga tgaaatcgcc aaacaggggc cgtatgagtt catctgtacg ggtcgcccgg
     2761 acgaaggcat cccggcggtt tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc
     2821 tgtacgacct ctctgaacgt ctgcgtctgc gcggctggca ggttccggcc ttcactctcg
     2881 gcggtgaagc caccgacatc gtggtgatgc gcattatgtg tcgtcgcggc ttcgaaatgg
     2941 actttgctga actgttgctg gaagactaca aagcctccct gaaatatctc agcgatcacc
     3001 cgaaactgca gggtattgcc cagcagaaca gctttaaaca cacctgataa cataacgttg
     3061 taaaaaccga atgcccagcc tttaaaaaaa cagctgggca ttcggttgct tattaatcgc
     3121 aataatatat tggctgtcta ttcatcgtgt tgataagata tatgttatat gaatgtttat
     3181 taaatgtagc gattaccttt aatgtcacga aaagggatta tttgcttgct attaatttca
     3241 ctgcgttctt aaacacgttt ttgtattata aatccagtca tcctgcccgt agaacatcac
     3301 acattatcat cctgttctcc cgcttcgttt aaatttattt atcaatcaat ttgacttaag
     3361 agggcggcgt gctacattaa ttaacagtaa tatgtttatg taatattaag tcaactatgc
     3421 aatcattaca tgggaattgt ctaattgcgt acgcaagaca taaatatatt ctcaccatgg
     3481 ttaatggtga atatcgctat tttaatggcg gtgacctggt ttttgcggat gcaagccaaa
     3541 ttcgagtaga taagtgtgtt gaaaattttg tattagtatc aagggatacg ctttcattat
     3601 ttctgccgat gctcaaggag gaggcattaa atcttcatgc acataaaaaa atttcttcat
     3661 tactcgttca tcactgtagc agagatattc ctgtttttca ggaagttgcg caactatcgc
     3721 agaataagaa tcttcgctat gcagaaatgc tacgtaaaag agcattaatc tttgcgttgt
     3781 tatctgtttt tcttgaggat gagcacttta taccgctgct tttgaacgtt ttgcaaccga
     3841 acatgcgaac acgagtttgt acggttatca ataataatat cgcccatgag tggacactag
     3901 cccgaatcgc cagcgagctg ttgatgagtc caagcctgtt aaagaaaaaa ttgcgcgaag
     3961 aagagacatc atattcacag ttgcttactg agtgtagaat gcaacgtgct ttgcaactta
     4021 ttgttataca tggcttttca attaagcgag tcgcagtgtc ctgtggatat cacagcgtgt
     4081 cgtatttcat ttacgtcttt cgaaattatt atgggatgac gcccacagag tatcaggagc
     4141 gatcggcgca gggattgccg aaccgtgact cggcggcaag tattgttgcg caagggaatt
     4201 tttacggcac taaccgttct gcggaaggaa taagattata gagttttact cagacataaa
     4261 aaaaacccgg cataggggac cgggaagagg atagtctgcc gtctccagac taataaaccg
     4321 ttataacact ccctgttggc acgggaaact ttgtgctctc agtaagttaa atataacttt
     4381 tacaggaaat aagatcagcc atttttttat aaacataagc tatacgctgt gcgaaaatat
     4441 attcgtgctg catttactta ttatcaatta actgttatgc aaaactactt tgtggataaa
     4501 ttttggtcct accaaatctg gcagtttttg cgctaagaaa cagtctgtca tcatttcatt
     4561 agtatactga aattgaaata atcgcagtat gaaatataag ggataatcat gactcatgtc
     4621 tgctcggtga tcctcattcg tcgttcattc gatatttatc atgaacagca aaaaatatcg
     4681 ctgcataacg agagtatcct gctgctggag aaaaatttgg cagacgattt tgcgttttgt
     4741 tcaccggata cgcgacgact ggatatcgat gagctgacag tttgccatta cttacaaaat
     4801 attcgtcagc taccacgcaa tttagggtta catagcaaag accgtctgtt aattaaccag
     4861 tcacccccca tgccgctggt gacggcgatt tttgatagct tcaatgaatc cggggtaaat
     4921 tcaccgatac tgagcaatat gctctacctt tcctgtttat cgatgttttc tcataagaaa
     4981 gaactgatcc ccttactttt caatagcatc agcactgttt caggaaaagt tgaacgcctt
     5041 attagctttg atatcgccaa acgttggtat ctgcgcgata tcgcggaaag aatgtatacc
     5101 agcgagagtc tcatcaaaaa aaagttgcag gatgaaaata cctgtttcag taaaatatta
     5161 ctcgcttcca ggatgtcgat ggccagacga ttactcgagt tacgtcaaat tcctctgcat
     5221 actattgcgg aaaaatgtgg ctatagcagt acgtcgtact ttataaacac atttcgacaa
     5281 tattatggtg taacgccaca tcagtttgcg caacattcgc caggtacctt ttcctgacat
     5341 attttgcatt tgaatattgg tcaggatctc acacctgctt catgtgaaac tcttccctga
     5401 tgatttctgc cgggctaccg gctagttctc tttcgcagtt tgccaggcgg tataggtatt
     5461 tttaggagga aaagactcgg catgtttggg attattaagc tgacaattca tacgattacg
     5521 ggaatgtggg tcagtattgt gttgttcaaa ttgatgacga atggctggag tggtttctac
     5581 tttcagtgct gtgtactaag cctggtattc ctgacggtta gctggttgtt gagtggtgaa
     5641 tggttagcag gaaagagtaa ggctgaacct tcacgttcaa ccttactctc atttacacgt
     5701 tacgcttttt taaagcgggc aaagagatgt tccactacaa cgaaaaagac cggaacgaag
     5761 taaattgcca gcactgttgc ggcaaacatc ccgcccatca cgccggtacc taccgcgttt
     5821 tgcgcgccag aaccggcacc atgactgata accagcggca gcacgccgag aataaaggcc
     5881 agagaggtca tcaggattgg gcgtaaacgc atccgcgccg cttcgatgat tgcctctatc
     5941 ggcgttttcc cttctttctg catcatctca acggcaaatt cgacaatcag gatggcgttc
     6001 ttggcggaaa gcccgatggt ggtcagcaaa ccaacctgga agtagacgtc attacttaag
     6061 ccgcgcagat cggtggccag taatgcgcca acgacgccta acggaacaac caacatcacc
     6121 gagaacggaa ttgaccagct ctcatagagt gcggcgaggg cgaggaacac cacgaccagt
     6181 gaaatggcat acagtgcagg agcctggttt gaggataacg cttcctgata cgacagtccg
     6241 gtccatgagt agccgacgcc tgccggaagt ttagcgacca ggtctgccat aaatttcata
     6301 gcgtcaccgg tacttttccc ggccgccgct tcacctaaaa tctccattga cgggatgccg
     6361 ttgtagcgtt ccaatcgcgg tgaaccatag gtccattcgg tagacgagta ggcagaaagc
     6421 ggtgccatcg tgccagaggc gttgcgtaca taccattggt tgatgttatc cggcaacata
     6481 cggaacggcg tgcctgcctg gacatacact tttttcaccc gcccctggtt gaggaagtcg
     6541 ttcacgtagc tgctgccgaa ggcggtggaa attgtctggt tgatatcaga cagcgccaca
     6601 cccattgcct cagctttcgc ggcgttgacg ttcactttga acatcggcgt atcttccagg
     6661 ccgttcgggc gtaccccggt gacctggtca ggcgattgcg ctgccagtga taacagctcg
     6721 tttcgtgcct aggttagttt ttcgtgcccc aggttaccgt tgtccagcag ttccatatca
     6781 aaacctgacg cggtacccag ttcagccacc gcgggtaagt tgaacgggaa gacgacggct
     6841 ttattgatac tgcttaacgc aatcattgcc cgctgaatga tcgcggtaac cgagttttcc
     6901 tcaccgacac gttcagacca cggcttgaga ctgataaacg ccaggccgtt gttttgcccc
     6961 tggccgctga agccaaagcc gccaacggta aacaccgact gaacattatc tttctctttt
     7021 gtaagataat aatccgtcac ctgttgcagt actttcgtgg tgttaaccat ggtggcaccg
     7081 gaaggtaact gcgcggtggt cataaatacc ccctgatcct cttctggtaa gaaagaggtc
     7141 ggtgtacgca ggaacagcac cgccatcccg gcgcaaatca gcaggtagac caccatgtag
     7201 cgaccggtac aacgcaacag cgagcgggtg ctatcggtat agtgctgagt tgatttttca
     7261 aacagcgtgt tgaagcgtgc gaacagggcg ttaggtttgt gaccgccttc cggcgtggct
     7321 ttcagaatgg tggcgcacag ggcaggggtc aggctcattg ccacaaatac tgaaagccgc
     7381 atggaggaga tcagcgtgat ggagaactgg cggtagatct ccccggttgc accgctcata
     7441 aaggccatcg gcataaacac tgcagaaaga acaacggcaa tcccgaccag cgcacgttgg
     7501 atctgcccca tcgatttatg cgtcgcttcc ttcggcggta gcttatcttc cgcaatgaca
     7561 cgctcgacgt tctccaccac cacgatggcg tcatccacca gtaaccctat cgccagcacc
     7621 atcccgaaca tagtcaacgt gttgatggtg aaaccgaccg ccgacaagat cgcaaacgtc
     7681 ccgagaataa ccaccggtac ggcaattgtc gggatgatgg tggcacggaa attctgcaaa
     7741 aacagataaa tgaccaggaa gacgaggatg atagcctcaa ccagtgtttt gaaaacttcc
     7801 tgaatagaaa tttcgataaa cggcgtggtg tcgtaaggat aaaccgtctt cagacttgcg
     7861 gggaaatagg ctgataagcg gttcagctcc tctttgactg cccgcgaggt atccagggcg
     7921 tttgctccgg cagccagttt gatggcgatc ccggcaacag gtttgccgtt atagcgcgcc
     7981 acggtggaat aatcttccgc cccaagttcg acgcgggcga catcacgcag cagcacttgc
     8041 gaaccatctt gctgaacttt caacaggatt ttgccaaatt cttccggcgt ttgcagacgc
     8101 gtctgcacaa tgatcgaggc gtttagttgc tggtctgccg cctgtggcat gccacccagt
     8161 tgaccaccag aaatctggtt gttttgcacc ttaatctggg aaataacatc ggaaggtacc
     8221 aggttatatt tattgagttt ttgcgggtcc agccagatac gcatggcgta ctcggaacca
     8281 aagagctgta cgctaccaac gcccgcggta cggcttagcg ggtctttgat attagacgct
     8341 acatagtccg cgatatcgta ctggttgagg ctgccgttat cagaaataaa cgccgctacc
     8401 atcaggatat tactgctcga cttatcgacg ctaatcccct gttgctgcac cgcttcaggt
     8461 aatgaaggca tagcgagttg cagtttattt tgcacctgaa cctgtgcgat atcaggagat
     8521 gccccagtct cgaaggtcag agtgatagag gcattacccg ccgcatcact ggttgaagac
     8581 atgtacatca ggccatcaag cccattcata ttttgctcaa tcacctgagt gaccgagtct
     8641 tctaccgttt gcgcatcggc acccggatag gtagcgctga cggtaatggt cggtggcgca
     8701 atctgcggat actgcgcaac cggtaagttc atgatcgcca gaccacctgc aagcatcata
     8761 ataatggcaa gtacccaggc aaaaaccggg cgatcaataa aatagttagc catgaaagtc
     8821 cccttaagcc tgcaacgtta ttgtttcgat tcggtgctgg cgttttcctg gctggaggaa
     8881 attgctcgtg ctttgatacc cggacgaatg cgttgcaaac cggaaacgat cacccgatcg
     8941 ccagcctgca agccagaggt gacaacccac tggtcgccga tggctttgct ggcttcaatt
     9001 tcgcgtagct gcacgacatc gtctttatcc agaatgagcg ccgttgcttt accctgggcg
     9061 ttgtgggtga cgccttcctg cggcaccagt aatataaagc gcgcaaaatt attcaagata
     9121 tttttgaaaa tcattcaaga taggctatcg cgtgtgatag caaaaacatt caagataaaa
     9181 atgcttgatt attaagcaat caggaatggg agtattaagt ttcgtgtttc cagtaaaacc
     9241 agagagtatt gaaatagcta tcacctaact ttatcaggcg atagctggta gatga
//
LOCUS       DAETQL010000137         9291 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.138, whole genome shotgun sequence.
ACCESSION   DAETQL010000137 DAETQL010000000
VERSION     DAETQL010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.138"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="K0W65_003732"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="K0W65_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HBI3096828.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="K0W65_003733"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="K0W65_003733"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBI3096829.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="K0W65_003734"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="K0W65_003734"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBI3096830.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="K0W65_003735"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="K0W65_003735"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HBI3096831.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="K0W65_003736"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="K0W65_003736"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBI3096832.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="K0W65_003737"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="K0W65_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBI3096833.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..6799)
                     /gene="nth"
                     /locus_tag="K0W65_003738"
     CDS             complement(6164..6799)
                     /gene="nth"
                     /locus_tag="K0W65_003738"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBI3096834.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="K0W65_003739"
     CDS             complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="K0W65_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HBI3096835.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="K0W65_003740"
     CDS             complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="K0W65_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBI3096836.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="K0W65_003741"
     CDS             complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="K0W65_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBI3096837.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
BASE COUNT         2384 a         2327 c         2357 g         2223 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg
     5821 cggcgatatg ttctgccatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac
     6361 gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt
     6421 ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat
     6481 cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt
     6541 ctgctttgct gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta
     6601 gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa
     6661 cactgatatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac
     6721 tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca
     6781 gacgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc
     6841 agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat
     6901 cagtcccaag ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt
     6961 gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc
     7021 aaacaacgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt
     7081 tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc
     7141 gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc
     7201 taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac
     7261 cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag
     7321 cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag
     7381 agcgttagtg gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag
     7441 tgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta
     7501 ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc
     7561 gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa
     7621 atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc
     7681 cgcaaaatgg gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt
     7741 ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag
     7801 cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg
     7861 tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt
     7921 taccagatag caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc
     7981 aaataacgtc ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt
     8041 gatggccgca gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt
     8101 tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta
     8161 gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg
     8221 atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag
     8281 ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag
     8341 catagtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa
     8401 ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg
     8461 aatcgctttc tgccatagca accacacgcc accagccagc caggcgagat ttacccattg
     8521 ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga
     8581 atgacctgcg cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc
     8641 aatacctaag cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac
     8701 ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct
     8761 ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa
     8821 cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc
     8881 cagtacgacc atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt
     8941 cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac
     9001 gagagcttcg gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca
     9061 accaaaaaac cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat
     9121 aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat
     9181 ttagtcctcg tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc
     9241 cgcggcaact gcggctttgc gcggatcgac ctgttcttct ggttccgcat t
//
LOCUS       DAETQL010000138         9197 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.139, whole genome shotgun sequence.
ACCESSION   DAETQL010000138 DAETQL010000000
VERSION     DAETQL010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9197
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.139"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..107
                     /locus_tag="K0W65_003742"
     CDS             <1..107
                     /locus_tag="K0W65_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3096838.1"
                     /translation="QHLARLVRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            124..1050
                     /locus_tag="K0W65_003743"
                     /pseudo
     CDS             124..1050
                     /locus_tag="K0W65_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1302..2219
                     /gene="oxyR"
                     /locus_tag="K0W65_003744"
     CDS             1302..2219
                     /gene="oxyR"
                     /locus_tag="K0W65_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBI3096839.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2202..3602)
                     /gene="sthA"
                     /locus_tag="K0W65_003745"
     CDS             complement(2202..3602)
                     /gene="sthA"
                     /locus_tag="K0W65_003745"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBI3096840.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3923..4573
                     /gene="fabR"
                     /locus_tag="K0W65_003746"
     CDS             3923..4573
                     /gene="fabR"
                     /locus_tag="K0W65_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBI3096841.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4573..4932
                     /locus_tag="K0W65_003747"
     CDS             4573..4932
                     /locus_tag="K0W65_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBI3096842.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4972..6072)
                     /gene="trmA"
                     /locus_tag="K0W65_003748"
     CDS             complement(4972..6072)
                     /gene="trmA"
                     /locus_tag="K0W65_003748"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBI3096843.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6186..6376
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6441..8285
                     /gene="btuB"
                     /locus_tag="K0W65_003749"
     CDS             6441..8285
                     /gene="btuB"
                     /locus_tag="K0W65_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBI3096844.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8230..9087
                     /gene="murI"
                     /locus_tag="K0W65_003750"
     CDS             8230..9087
                     /gene="murI"
                     /locus_tag="K0W65_003750"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBI3096845.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2103 a         2224 c         2480 g         2390 t
ORIGIN      
        1 gacaacatct ggcaaggctg gtacggaagt cactgtcgtt ctcaaaatcg gtggagctgc
       61 atgacaaggt catcgggcat tatctgaaca taaaacacta tcagtaagtt ggagtcatta
      121 cccggtctgg gtattggcga agcgccattt atgcctgcgg gagtaaagtc gatcaccgac
      181 tggtatgcac aaaaagaacg cggcacggcg ctggggattt ttaactcatc taccgttatc
      241 ggtcaggcta tcgcgcctcc tgctcttgta ttgatgcagc tggcatgggg ctggcggacg
      301 atgttcgtta tcatcggcgt ggcagggatt ctggttggga tctgttggta cgcgtggtat
      361 cgcaaccggg cgcagtttgt cctgactgac gaagaacgga cgtatctctc cgcgtcggtt
      421 aaaccgcgtc cacagctgca atttagcgag tggctggcgc tgtttaagca tcggacaacc
      481 tgggggatga ttttaggttt ctctggcgtc aattataccg gttggcttta cattgcgtgg
      541 ttacccggtt atttgcaagc cgaacaaggt ttcagtctgg cgaaaaccgg ttgggtagcg
      601 gcgattcctt tcctcgcggc ggcggtcggg atgtgggtta acggtattgt ggtcgatcgg
      661 ctggcgaaaa aaggctacga cctggcgaag acgcgtaaaa cggctattgt ctgcggtttg
      721 atgatgtcgg cattaggcac gttgctggtc gtgcaatctt cctcgccagc tcaggcggta
      781 gcgtttatct caatggcgct gttctgcgtg catttcgcgg gaacgtctgc atggggactg
      841 gtgcaggtga tggtgtcaga aacaaaagtg gcttccatcg ccgggattca aaactttggc
      901 agttttgttt ttgcttcctt tgctccgatc gtaaccggtt gggtagtgga taccacacac
      961 tcgtttaatc tggcgctggt tatcgcggcc tgtgtgacgt tcaccggggc gctgtgttac
     1021 ttctttattg tcaaagatcg cattgagtaa tcgttactat caggctctct tgctgtgggc
     1081 ctgtagaata aaaaaaagcg gatcctggag atccgcaaaa gttcacgttg gctttagtta
     1141 ttcgagttga gaaactctcg aaacgggcag tgacttcaag ggttaaaaga ggtgccgctc
     1201 cgtttctgtg agcaattatc agtcagaatg cttgataggg ataatcgttc attgctattc
     1261 tacctatcgc catgaactat cgtggcgatg gaggatggat aatgaatatt cgtgatcttg
     1321 agtacctggt ggcattggct gaacaccgcc attttcggcg tgcggcagat tcctgccacg
     1381 ttagccagcc gacgcttagc ggccaaattc gtaagctgga agatgagctg ggcgtgatgt
     1441 tgctggagcg gaccagccgt aaagtgttgt tcacccaggc gggaatgctg ctggtggatc
     1501 aggcgcgtac cgtgctgcgt gaggtgaaag tccttaaaga gatggcaagc cagcagggtg
     1561 agacgatgtc cggaccgctg catattggtt tgattcctac ggttggacca tacctgctac
     1621 cgcatattat cccgatgctg caccagacat ttccaaagct ggaaatgtat ctgcatgaag
     1681 cacagaccca ccagttactg gcgcaactgg acagcggcaa actcgattgc gtgatcctcg
     1741 cgctggtgaa agagagcgaa gcattcattg aagtgccgtt gtttgatgag ccaatgttgc
     1801 tggctatcta tgaagatcac ccgtgggcga accgcgaatg cgtactgatg gccgatctgg
     1861 caggggaaaa actgctgatg ctggaagatg gtcactgttt gcgcgatcag gcaatgggtt
     1921 tctgctttga agccggggcg gatgaagata cacacttccg cgcgaccagc ctggaaactc
     1981 tgcgcaacat ggtggcggca ggtagcggga tcactttatt gccagcgctg gctgtgccgc
     2041 cggagcgcaa acgcgatggg gttgtttatc tgccatgcat taagccggaa ccatgccgca
     2101 ctattggcct ggtttatcgt cctggctcac cgctgcgcag ccgctatgag cagctggcag
     2161 aggccatccg cgcaagaatg gatggccatt tcgataaagt gttaaaacag gcggtttaaa
     2221 ccgtttaacg cagctacccg ataggcttcc gccatcgtcg ggtagttaaa ggtggtgttg
     2281 acgaagtact caatagtgtt gccgccacct ttctgttcca taatcgcctg accgatatga
     2341 ataatttcgg cagcgcgctc gccaaagcag tgaataccca gaatctcttt tgtttcccga
     2401 tggaacaaaa ttttcagcgt gcccacgttc atgccgacga tttgtgcacg tgccagatgt
     2461 ttgaactggg cgcggcccac ttcatacggc actttcatcg cggtcagctg ctgttcggtt
     2521 ttgcccacag agctgatttc cgggatggtg taaatgccgg tagggatatc ttcaatcaga
     2581 tgtgcggtgg cttcgccttt taccagcgcc tgcgcggcaa tgcgcccctg gtcataggcc
     2641 gctgatgcca ggctcggata accaatcacg tcgcccaccg cgtaaacgtg cggctgtgcg
     2701 gtctgataca tgctgttgac cttcagctgt ccgcgactgt cagtttccag cccaatgttc
     2761 tgtaacgcca gcgaatcggt attaccggta cgaccgttgg catagagcag gcagtcagct
     2821 ttcagttttt tacccgactt cagatgcatg atcacaccgt cgtcacagct ttcgatcttc
     2881 tcgtactctt cgttgtgacg aatcactacg ccactgttcc agaagtggta ggagagagaa
     2941 tctgacatct cttgatcgag aaatgccagc aggcgatcgc gggtgttgat cagatccact
     3001 tttacatcca taccgcggaa gatcgacgca tattcacagc cgatcactcc agcaccatag
     3061 ataagtacat ggcgcggttc gtggtgcatg ctaagaattg agtcgctgtc gtaaatgcgt
     3121 ggatgggtga aatcaacatc tgttggatga tatggacgag agccgcaggc aataacaaat
     3181 ttttcagcgg ttagtgtttc aacgctgccg tccgggcaat ccagcgccaa cgtatgctcg
     3241 tcaacaaagc gagcgttccc ctgcaatatt tcacagtgat tacgttcgta aaatccctga
     3301 cgcatgcgcg tttgttgatt aatcacgtta tcggcatggt taaggatatc ggcaaaagaa
     3361 gagcggagca gtcgggaatg gtcgctgtat agtgggtttt gattgaattc tataatgcgg
     3421 ctgacggcgt gacggagagc tttcgacggg atggtgcccc agtgggtgca accgccgcca
     3481 acattttgat aacgctcgat aactgcgacg cgcgcacctt gcttaaccag gcccattgca
     3541 gcgccttcgc cgccggggcc ggaacctatt actatggcat cgtaatcgta ggaatgtggc
     3601 atggtagggc ttacctgttc ttatacataa aagcaacaga atggtaacat tctatcgcgg
     3661 gtaagccaat tgatccccgt catttatctg gctatatcct gagcggcctt tgctttgtct
     3721 gtttcttact tttgccctga cgttttattg gatttttatc gacgatactc tccgtttaag
     3781 cggcaggttt ccgctgtacg taaaagaacc ggccagtaaa tatgttttat tgcgttaccg
     3841 ttcattcaca atactggagc aatccagtat gttcattctc tggtatagtg ccagcagtac
     3901 ttttggcaag gattcagaca tcgtgatggg cgtaagagcg caacaaaaag aaaaaacccg
     3961 ccgttcgctg gtggaagccg catttagcca attaagtgct gaacgcagct tcgccagcct
     4021 gagtttgcgt gaagtggcgc gtgaagcggg cattgctccc acctcttttt atcggcattt
     4081 ccgcgacgta gacgaactgg gtctgaccat ggttgatgag agcggtttaa tgctacgcca
     4141 actcatgcgc caggcgcgtc agcgtatcgc caaaggcggg agtgtgatcc gtacctcggt
     4201 ctccacattt atggagttca tcggtaataa tcctaacgcc ttccggttat tattgcggga
     4261 acgctccggc acctccgctg cgtttcgtgc cgccgttgcg cgtgaaattc agcacttcat
     4321 tgcggaactt gcggactatc tggaactcga aaaccatatg ccgcgtgcgt ttactgaagc
     4381 gcaagccgaa gcaatggtga caattgtctt cagtgcgggt gccgaggcgt tggacgtcgg
     4441 cgtcgaacaa cgtcggcaat tagaagagcg actggtactg caactgcgaa tgatttcgaa
     4501 aggggcttat tactggtatc gccgtgaaca agagaaaacc gcaattattc cgggaaatgt
     4561 gaaggacgag taatgaaaca agcaaatcaa gatagaggta cgctgctgct ggcgttggtt
     4621 gctggtttat cgattaatgg tactttcgca gcgctgttta gctccattgt gccattttct
     4681 gtattcccga ttatttccct ggtgctgacg gtttactgcc tgcatcaacg ttatcttaat
     4741 cgcaccatgt cggtaggctt gccgggtctg gcagctgcct gttttattct cggcgtactg
     4801 ctgtacagca cggtagttcg tgcggaatat ccggatatcg gctctaactt cttcccggca
     4861 gtactctccg tcattatggt gttctggatt ggcgcgaaga tgcgtaaccg taagcaggaa
     4921 gttgctgagt aatcggtatc atgccggatg ctggcgcatc cggcatgggt tttacttcgc
     4981 ggtcagtaat acgccgcact ccatatggtg cgtgtagggg aactgatcaa acagagccag
     5041 acgttcgacc ttgtgcgtct ggcttaatgt ttccagattt ttgcataacg tttccggatt
     5101 gcaggagata tacagaatac gtggatacgc ctgcaccatt ttctcggttt cactgtccag
     5161 accgctgcgc ggtgggtcga caaaaatggt ttcgaactga tagctcttta agtcgatccc
     5221 ttgcaggcgg ttaaactcgc gcacaccatt catcgcctga gtaaattctt ctgccgccat
     5281 acgaataatt tgtacgttat caatatggtt agcggcaatg ttgtattgcg cggcagcgac
     5341 tgacggtttg gcgatttcgg tggctaatac ccgatcaaaa ttgcgcgcca gcgctaatga
     5401 aaagttaccg ttgccgcagt acagctccag taaatcgcct tttgagcctt tggttacgtc
     5461 cagcgcccat tccagcatct gaatattcat cgccgcgttc ggctgggtaa agctgttttc
     5521 tacctgacga tagatcatct ctttccctgc gaccggcaga cgctcgtcga tgtaatcttg
     5581 atccagcgcg attttggttt tcgttgcccg accaatcaga tgcacattca gattctgcgc
     5641 gcgcagtgca tcgcgcagtg cttccgcttc ctgacgccac tcatcatcca gctttttatg
     5701 gtacagcagg gaaaccaccg cctgattact cagcgtggtg aggtaatcaa tctggaacaa
     5761 cttgtggcgc agaacgggat tattacgcac acccgcaatc atcgccgtca tcaactggtt
     5821 gatgagttca ctggcggcgg ggaagctatc cacgcggatg cggcttttgg tttgttgatc
     5881 gaaaatgatg tgatacaggt catcgccatc gtgccagatg cggaactccg cgcgcatccg
     5941 gtaatgactg accggcgagc gaaacacttc cggaaccagg tcagaaaacg gtgccatcat
     6001 actttgcaaa cgtaccactt tttcggctaa ctgcgcttca tactgttctg ttggaaggtg
     6061 ttcgggggtc atgatgtatc ctgaaaaatt aaagtacgcg gcgattgtag ggattgctca
     6121 tcagatgtcc agatcttgat gaattcctat ttgtgagcta cgtctggaca gtaacttgtt
     6181 acaacctgta gcatccactt gccggtcctg tgagttaata gggaatccag tgcgaatcta
     6241 gagctgacgc gcagcggtaa ggaaaggtgc gatgattgcg ttatgcggac actgccatcc
     6301 ggtgggaagt catcatctct tagtatctta gatacccctc caagcccgaa gacctgccgg
     6361 ccaacgtcgc atctggttct catcatcgcg taatattgat gaaacctgcg gcatccttct
     6421 tctattgtgg atgctttaca atgattaaaa aagcttcgct gctgacggcg tgttccgtca
     6481 cggcattttc cgcttgggca caggatacca gcccggatac tctcgtcgtt actgctatcc
     6541 gttttgaaca gccgcgcagc actgtgcttg caccaaccac cgttgtgacc cgtcaggata
     6601 tcgaccgctg gcagtcgacc tcggtcaatg atgtgctgcg ccgtcttccg ggcgtcgata
     6661 tcacccaaaa cggcggttca ggtcagctct catctatttt tattcgcggt acaaatgcca
     6721 gtcatgtgct ggtgttaatt gatggcgtcc gcctgaatct ggcgggggtg agtggttctg
     6781 ccgaccttag ccagttccct attgcgcttg tccagcgtat tgaatatatc cgtggaccgc
     6841 gctccgctgt ttatggttcc gatgcaatag gcggggtggt gaatatcatc acgacgcgcg
     6901 atgaacccgg aacggaaatt tcagcagggt ggggaagcaa tagttatcag aactatgatg
     6961 tctctacgca gcaacaactg ggggataaaa cacgggtaac gctgttgggc gattatgccc
     7021 atactcatgg ttatgatgtt gttgcctatg gtaataccgg aacgcaagcg cagacagata
     7081 acgatggttt tttaagtaaa acgctttatg gggcgctgga gcataacttt actgatgcct
     7141 ggagcggctt tgttcgcggc tatggctatg ataaccgtac caattatgac gcgtattatt
     7201 ctcccggttc accgttgctc gatacccgta aactctatag ccaaagttgg gacgccgggc
     7261 tgcgctataa cggcgaactg attaaatcac aactcattac cagctatagc catagcaaag
     7321 attacaacta cgatccccat tatggtcgtt atgattcgtc ggcgacgctc gatgagatga
     7381 agcaatacac cgtccagtgg gcaaacaatg tcatcgttgg tcacggtagt attggtgcgg
     7441 gtgtcgactg gcagaaacag actacgacgc cgggtacagg ttatgttgag gatggatatg
     7501 atcaacgtaa taccggcatc tatctgaccg ggctgcaaca agtcggcgat tttacctttg
     7561 aaggcgcagc acgcagcgac gataactcac agtttggtcg tcatggaacc tggcaaacca
     7621 gcgccggttg ggaattcatc gaaggttatc gcttcattgc ttcctacggg acatcttata
     7681 aggcactaaa tctggggcaa ctgtatggct tctacggaaa tccgaatctg gacccggaga
     7741 aaagcaaaca gtgggaaggc gcgtttgaag gcttaaccgc tggggtgaac tggcgtattt
     7801 ccggatatcg taacgatgtc agtgacttga tcgattatga tgatcacacc ctgaaatatt
     7861 acaacgaagg gaaagcgcgg attaagggtg tcgaggcgac agccaatttt gataccggac
     7921 cactgacgca tactgtgagt tatgattatg tcgatgcgcg caatgcgatt accgacacgc
     7981 cgttgttacg ccgtgctaaa cagcaggtga aataccagct cgactggcag ttgtatgact
     8041 tcgactgggg tattacttat cagtatttag gcactcgcta tgataaggat tactcatctt
     8101 atccttatca aaccgttaaa atgggcggtg tgagcttgtg ggatcttgcg gttgcgtatc
     8161 cggtcacctc tcacctgaca gttcgtggta aaatagccaa cctgttcgac aaagattatg
     8221 agacagtcta tggctaccaa actgcaggac gggaatacac cttgtctggc agctacacct
     8281 tctgaaccac gtcccaccgt gctggtgttt gactccggcg tcggtgggtt gtcggtctat
     8341 gacgagatcc ggcatctctt accggatctc cattacattt atgctttcga taacgtcgct
     8401 ttcccgtatg gcgaaaaaag cgaagcgttt attgttgagc gagtggtggc aattgtcacc
     8461 gcggtgcaag aacgttatcc ccttgcgctg gctgtggtcg cttgcaacac cgccagtacc
     8521 gtttcacttc ctgcattacg cgaaaagttc gacttcccgg ttgttggtgt cgtgccggcg
     8581 attaaacctg ctgcacgtct gacggcaaat ggcattgtcg gattactggc aacccgcgga
     8641 acagttaaac gttcttatac tcatgagctg atcgcgcgtt tcgctaatga atgccagata
     8701 gaaatgctgg gctcggcaga gatggttgag ttggctgaag cgaagctgca tggcgaagat
     8761 gtttctctgg atgcactaaa acgtatccta cgcccgtggt taagaatgaa agagccgcca
     8821 gataccgttg tattgggttg cacccatttc cctctactac aagaagaact gttacaagtg
     8881 ctgccagagg gaacccggct ggtggattct ggcgcagcga ttgctcgccg aacggcctgg
     8941 ttgttagaac atgaagcccc ggatgcaaaa tctgccgatg cgaatattgc cttttgtatg
     9001 gcaatgacgc cagaagctga acaattattg cccgttttac agcgttacgg cttcgaaacg
     9061 ctcgaaaaac tggcagtttt aggctgattt ggttgaatgt tgcgcggtcg gaaaattatt
     9121 ttaaatttcc tcttgtcagg ccggaataac tccctataat gcgccaccac tgacacggaa
     9181 caacggcaaa cacgccg
//
LOCUS       DAETQL010000139         9170 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.140, whole genome shotgun sequence.
ACCESSION   DAETQL010000139 DAETQL010000000
VERSION     DAETQL010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9170
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.140"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(227..2770)
                     /gene="mdoH"
                     /locus_tag="K0W65_003751"
     CDS             complement(227..2770)
                     /gene="mdoH"
                     /locus_tag="K0W65_003751"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBI3096846.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2763..4298)
                     /gene="mdoG"
                     /locus_tag="K0W65_003752"
     CDS             complement(2763..4298)
                     /gene="mdoG"
                     /locus_tag="K0W65_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBI3096847.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4692..5849
                     /gene="mdoC"
                     /locus_tag="K0W65_003753"
     CDS             4692..5849
                     /gene="mdoC"
                     /locus_tag="K0W65_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HBI3096848.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5857..7338)
                     /gene="clsC"
                     /locus_tag="K0W65_003754"
     CDS             complement(5857..7338)
                     /gene="clsC"
                     /locus_tag="K0W65_003754"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HBI3096849.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7280..7813)
                     /gene="ymdB"
                     /locus_tag="K0W65_003755"
     CDS             complement(7280..7813)
                     /gene="ymdB"
                     /locus_tag="K0W65_003755"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBI3096850.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7908..8219)
                     /locus_tag="K0W65_003756"
     CDS             complement(7908..8219)
                     /locus_tag="K0W65_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI3096851.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8340..8672)
                     /gene="csgC"
                     /locus_tag="K0W65_003757"
     CDS             complement(8340..8672)
                     /gene="csgC"
                     /locus_tag="K0W65_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBI3096852.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8731..9096)
                     /gene="csgA"
                     /locus_tag="K0W65_003758"
     CDS             complement(8731..9096)
                     /gene="csgA"
                     /locus_tag="K0W65_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBI3096853.1"
                     /translation="MPVHLSNNSGPNSELNIYQYGGGNSALALQTDARNSDLTITQHG
                     GGNGADVGQGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASN
                     SSVNVTQVGFGNNATAHQY"
BASE COUNT         2297 a         2368 c         2180 g         2325 t
ORIGIN      
        1 gagagtggta aaggagaggc cctggaaata ccccacagcc ccgaaaaaga gccagtcagt
       61 tgcttaactg actggcatta cggcataacg ccggtatttt tattggctcg ttgtttttgc
      121 cggatgtggc gtgaacgcct tatccgacct acaaaatctt gcaaaatcaa taaattgcag
      181 gaacgatgta ggcctgataa gcgtagcgca tcaggcaact acgtttttat tgcgaagccg
      241 catccggttt acgcaatgcc agtggattga gctttatccc ttcgtaataa ctcacccatg
      301 aagaatatct ttcaggggaa ttccagacgc ggaaatgcag acgggccatc gtcaccggat
      361 cgcttagcag caccaggcga cgatcgcgat tcagcttctc tggcgtctcg ttaagcgcct
      421 gttcaacgtg gcggtcacgg gcgatttcca gcaccttact ggcgcggtga cgcgctgtcg
      481 ccattgcggt tgccagagcg ttaaatgacg ggttaaacac tgcgtgcata aagccatcgt
      541 caagggagcg ttggcgattc atctcaagga accgatcggt atcaaccagc acctgcggcg
      601 gtgaatactc ttccgggatc aggaacagtt tccagcgttt ggtgcgcaga ccaacggtgg
      661 cacggctgga aatcaccgaa acaaacggtg acaggatcaa cgagaagaca atcggtgcca
      721 gccagaacag gaaacgcaga tccagccacg ccatcccaac agcccacact aaccccaaca
      781 gcagctgtga gccgtggcgt ttgaacgctt cgccccatga agtggagtca tcatcacgct
      841 gcggtgaatt ccacaccact tcccagccaa ggaacgcgct gacaacgaag accgtatgga
      901 acagcatgcg taccggagcc agcagcacgg aaaacagcac ttccagcagc aacgataatg
      961 taacgcgcca gaagccgccg tattctttcg ttcctttgca ccagataagc aaaatgctca
     1021 ataacttcgg caggaacaac agcaccatgg tcgaagcaaa aagtgcaatc gccagctcag
     1081 gacgccactg cggccacacc gggaacaact gccgtggttg caggaagtat tgcggttcgg
     1141 tcaacgcatg cactacctgc aatgcagtag agagcgcgag gaacataaac cacagcggag
     1201 cggagagata agacatcacg cccgtcagga acaccgcacg gtgaaccggg tgcataccct
     1261 taaccaggaa cagacggaag ttcatcaggt taccgtggca ccagcggcgg tcacgtttta
     1321 gctcatcaag caagttaggc ggcaattctt cataagaacc cgggagatcg taagcaatcc
     1381 agacccccca acctgcacgg cgcatcaacg ccgcttctac gaagtcatgt gacaggattg
     1441 aaccggcaaa ggaaccttcg cccggcagcg gagccagtgc gcagtgctcg ataaatggtt
     1501 tcacgcggat aatcgcgtta tgtccccagt agtgcgactc gccaagttgc cagaagtgca
     1561 aaccggctgt aaacagtggc ccatacacgc gggtcgcgaa ctgctgacag cgcgcataca
     1621 gcgtatccat accggacgct ttcggcgacg actgaatgat cccggcgttc gggttggctt
     1681 ccatcaagcg caccaggccg cataaacaat caccggtcat taccgagtca gcatccagca
     1741 ccaccatgta gctgtactgg ctgccccagc gacggcagaa gtcatcgata ttaccgcttt
     1801 tacgcttcac gcggcgacgg cggcggcgat agaaaatctg accttcgcca ccgacttcag
     1861 cgataagctc catccaggct ttttgctctg cgacgcagat atccgggtta taactgtcac
     1921 tcagaatgta gacatcaaag tgtttggcat tcccggtggc ttttactgat tcccacgttg
     1981 cacgcaagcc agcaaaaaca cggttcacgt cttcgttaca gataggcatg atcaacgccg
     2041 tgcgatgctc cgggtttaat ggttcatcgc caactgttga cgcagatata ctgtatttat
     2101 cgcgaccaat aagcagttgc aggaagccca tcaacgccgt ccagaatccg gcggacaccc
     2161 aacagaacag taccgcaaaa aggatcagga taccggtttg cagcatataa ggcagaagct
     2221 gcataaagga aacccacaaa tcctgaccaa ccatatccat aggattaatc agcgcccaac
     2281 cctgataagg aagaatggtc ttcatatacc aggtcgcgac aacagtttgc gcgagcgtca
     2341 ggatcaacag aatgtaacgg cggatggtac caacggtacg ccacttttgc tcgctctcct
     2401 gctcttcttt ggtcaaacga gccagatagc gcggcgtgac atcgcgtcca cgcaggcgat
     2461 cccagaaacg gcctaccggg ttggtacgcc acgggtcggg aaacatcgag gagcgttttg
     2521 cttctggcat cgccttaagc tgatcgcgcc cttcgtcgtc tttaattaac tgtccatcag
     2581 caagtgaatc tggccaggct tgttccagac gcgcctttac cgagccttgc ggggaatcat
     2641 cctcccgcgc ccaggtgcgg tgttcggcat ccagcgcctg atgaacggcg cggatatcag
     2701 tcttcggcaa tgctgctttc tcgcttgcgg cgatgggcat tgcgtcaatg tactcagttg
     2761 tcttattcat tggcaggtaa ctggtagctc caggtttcac tcaacgtctg atcggcattc
     2821 accagcgcag cacgcatttc agtgattttc ttggcatctt tcactttcac acgcatcacc
     2881 agacgccagc ctttggtaac cgggttatag cgcaccgtgc tttcaactat ctcaccatta
     2941 tcaccaatgc tggtttgcgc tgtgaccggg gtatcctctg gcagtttttt catctctgcg
     3001 ccggtaaaat cgaccacaaa ggcgatagta ccgtcaggct ggcgaatcag gttcgactgc
     3061 ttcacatccc ccgttgaacg acgcgtttgt tgcacccatg cgttatctgg cgcatgcagt
     3121 ttgtcttcat cacggctgaa ggtgatggtg tatttaaagt tcatctcttt acccggctcc
     3181 ggcagctgat ccggcgtcca gtaagcgacg atgttatcgt tggtttcatc gttggttgga
     3241 atttccacca gctcaacgct gcctttgccc cactcccctt tcggagtcac ccatgcgctt
     3301 ggacggagat cgtaacgatc atcgagatct tcaaagcggg agaaatcacg acctcgttgc
     3361 aacagaccaa agcctcgcgg gttttccatg gagaagctgc tgaccgctaa atgtttcggg
     3421 ttattcaacg gacgccagat ccattcgcca ttaccagcat ggatagacag accgttagag
     3481 tcgtgcaact ccggacgata gttatttgca ggcgacggtt ggttcggccc aaacaggaac
     3541 atactggtta acggtgcaac ccccagtttg ccgactttat cgcgcagata gattttcgac
     3601 tgcacatcca caaccgtgtc acgccctggc ataaccacga atttgtaagc acctgtcgca
     3661 cgcggcgagt caagcaatgc ataaatggtt aaacgtttat cagtcggttt tggacgctcg
     3721 atccagaact ctttgaagcg tggaaattct tcacccgatg gcaaagcggt atcaattgct
     3781 aggccgcggg cagaaaggcc ataaacctga cctgcaccaa tcacgcggaa atagctggcc
     3841 ccgagcatgc tgacgatttc atcgttttta tctttgctgt tgatcgggta aagcacttta
     3901 aaaccggcaa aaccaaggtc ttttaccgtg tctttgtcat gctgaacatc gccgaaagtg
     3961 aaataatccg ggctgtattt gattcgtttg actgcggtgg cagtcacttc atttattttg
     4021 accggggtat cgaagtacat accctgatgg tagaactcga gtttgaatgg ggtcttcaga
     4081 ttgttccagt acgctttgtc atgattaaac tggatctgct gatagtccgc gtatttcatg
     4141 tcgcggaaaa cggagggcaa gttgctttta ggcgcctcat agcctttccc ggctaaggat
     4201 tgagcttgct ttgcgacatc atcaatactg aaagcccagc ttgaagatgt atacagggtt
     4261 aacattactg cagcactcaa ccaacgcatt ttcatcattt gtagtttatg tttcataatt
     4321 agtaagcact tccccctttg tgtgcttata tcgatccgat ctattttaat ggataaccag
     4381 ccattccgac aactgaatcc cggtatcgtt cagcacgctg gctcatggat taagcacctg
     4441 attgcaaccg ttttcacttt ccgtgcttct cctaaagaca gaagcatcga cttcgtgtag
     4501 ggtttgccag caaatttatt tatcaaccgt cttacagacg cttccattct ggttagtggt
     4561 agcgatgatg ctatgcataa aaaccttgaa gcctgcgcct ggcgcggtca ggtcggtgtc
     4621 gctatggcaa caagcctaaa ggcataaccc gacagggata agacgaaaaa tccgagatta
     4681 gttaaccata tatgaatcca gtacccgcgc aacgtgaata tttcctcgac tccatccgcg
     4741 cctggctgat gttgttaggg atcccttttc atatttcttt aatctattcg agccatacat
     4801 ggcatgtgaa tagcgccgaa ccgtcattgt ggctaaccct ttttaatgac ttcatccact
     4861 cgttccgcat gcaggtattt ttcgttatat ccggatactt ttcctacatg ctttttttac
     4921 gctatccctt gaaaaaatgg tggaaagtac gtgtcgaacg tgtaggtatc ccgatgttaa
     4981 cagccatccc cctactgaca ttaccgcaat ttattatgct gcaatatgtc aaagggaaag
     5041 cggaaagttg gcctgggcta tcattgtatg acaaatataa tacgttggcc tgggaattaa
     5101 tatcacacct gtggttttta ctggtgttag tagtcatgac gacgctgtgt gtatggatat
     5161 ttaagcgcat cagaaataat ttagaaaatt ctgataaaac gaataaaaaa ttctcgatgg
     5221 taaaactatc ggtgattttt ttatgcctcg gcatcggcta tgcggtaata agaagaacga
     5281 tttttattgt gtatccaccc attctgagta atggcatgtt caattttatt gtcatgcaaa
     5341 cgctatttta tttaccgttc tttatcctcg gcgcactggc tttcattttc cctcatctta
     5401 aagccttgtt taccacgccg tctcgtggct gtaccctcgc tgcagcattg gcgtttgtcg
     5461 cttatttact caaccagcgc tatggcagtg gcgatgcctg gatgtacgaa accgagtcgg
     5521 tgatcaccat ggtcctcggc ctgtggatgg tgaatgtggt cttctccttt ggccaccgtt
     5581 tgcttaactt ccagtcagcg cgggtgactt attttgttaa cgcatcgctg tttatctatc
     5641 tggttcacca cccgttaacg ctgtttttcg gcgcatacat tacaccgcac atcacctcca
     5701 actggcttgg ttttctctgt ggcctgatat tcgtagtagg gattgcgata attctgtatg
     5761 aaattcattt acgcatcccg ttactgaagt ttttgttctc tggtaaaccg gttgttaagc
     5821 gtgagaacga taaagcacca gcccgttaag ccacatttac aataaccatt ccacgggcaa
     5881 tatcgacgcc agtctgacca taacccgctt ccagaaactg gtggcgggtt cttttttgag
     5941 aataacctct ttctttgcat gacgatcaac ccagttgatc cgtccccacc tgtccagacg
     6001 gagctgccag gccgcatcgt actggctctg aataaagcgt ttatcaatta actgtgccag
     6061 cgtttcgctc tctatcacga agcccatttc agtattgagc aatgttgaac gcggatcgaa
     6121 attgaaagag ccgataaaca ccgttttacc atcgatgcta aaagttttag catgcaggct
     6181 ggcaccggaa ttaccggtta tgccgcgatc gtgtaacgta ctgctttgtt cacgcgtcgg
     6241 cttgagttca tataattcca cgccatagcg gagcaatttt ttgcgccagc gcgcgtatcc
     6301 ggcatggacg acagcaacat cgttagcggc aagagaattg gttaggatcg caatctttac
     6361 cccttttctc accatccgta agagttgcgc cacacctgcg cgtgtcggta caaaatagga
     6421 agagataata tcgatgcgtt cactgggtga gcccatgata tcgaacaggc gctgcggtaa
     6481 cagtgaatgc cgttttgcct tgccctcccc tttcgccgga tcatcactta ataaacgtgt
     6541 cttcgcccag ataagcggca acgttccatc aaccagatga tttataaatg gactggattc
     6601 cattttgcgt aaataacgat gcgtcatggc atcgttatgc caggaggcgg gtaactcgat
     6661 gcgatccgcc atttcacctt ccgggacatc cagcacctgc tgtaagggtg aaaccgattt
     6721 gcaataccag tagcgggcga aatcatcggc aacatcctct accacggggc ctattgccat
     6781 gacatctaaa tccgaaaaaa gtggctcttc ccctgcacca aaataggcat caccaatatt
     6841 tcgtcctccc actagggtaa ccacgccatc gacagtgaaa cttttattgt gcatccggcg
     6901 attgagacgg gaaaagtcgg tgatatatcc aagcggacgt agcaagcgaa acgagaaagg
     6961 attaaaaagc cggacttcaa tgcgtggatg actgtcaagc aagcgcaaaa tgtcgtcaag
     7021 tccgggcgta ttgttgtcgt ccagcaacaa acggacgcga acgccacgct ttgctgcggc
     7081 taacagggcg gaaaacagta accgccccga catatcgtcc tgccagatgt aatactgaac
     7141 atccagcgta tgctctgcca tttcggccag acgataccgg gcggcaaacg catccagact
     7201 tttctccaga gggaaaagac cacactgacc gggatgttgc gaacacagtg gcagcaccgc
     7261 gctcgccagc cggggcaaat cattcatctc cttgttgggt aaggagtctt tcgtagaggt
     7321 gggcgttttc ttcatcataa cagacaaagt atacctgttc gggtaaagcg tgacgggtaa
     7381 taaattctga aacggtttta actgcgattt cagccgctgc cgcacgaggg taactataaa
     7441 ccccagtact gattgcagga aaagccactg acgtatagct gtttgccgcc accagtcgta
     7501 ggctattcag ataggcatcc tgcaaaagct ggtcttcgtt ttgttcccca ccacgccaga
     7561 ctggcccgac ggtgtgtact acggctttag cgggaagatc gcctgcaagc gtaataacgg
     7621 catgccccgt agggcaatcg ccctgctgtt gcctgacttt taaacaggca tccagcaggg
     7681 ccggacccgc tgcgcgatga atggccccat cgacgccgcc gcctcccatt aatgacggat
     7741 tagccgcatt cacaatcaca tcaacggcca gtttggtaat atcaccctgc acaacatgaa
     7801 tacgcgtttt cataacgcct ccttacacct tcttgtttct taagtgtaca gcaggctttt
     7861 tggttaaatc gcatttttct gctcagcaac gtgagttgtt cagaaactta gttatattcg
     7921 atgttcatta cagccagctt tttctgttta tcaagatact gcacttttac tgaggctatt
     7981 tgcgcgtttt tgacattgcc gctcattaac gctttgctgg agtaggtttt gcccggaata
     8041 gaaccattct gtgggcagtt catttctacg ggtgatgact gggtgctgac gtcacatgcc
     8101 ggttccacaa tttggccata aaaatggatc acgccccctt gcgcggtgct tcctgcaatg
     8161 gcaataaaag gaaaaaacaa actgccgagc ataagagagt ctaaaaaagg tctgaacatt
     8221 tattctattt cctcaatgat tagtcatcct tgaaggttgt gttatccata ctttcaggtt
     8281 ggctgattcg cagcagacca ttctctccag attcatctta tgctcgatat ttcaacaaat
     8341 taagactttt ctgaagaggg cgtccattgt tgtgataaat gaagtgcctg tccatcagaa
     8401 acagtaacaa ctattttcac ccgatcttcc ggggaaatat ttaaactcaa cttcgtcaaa
     8461 gcaatgggtt gattagcagg caatgagagg gtcttttctt gcttcgtctg actttgccct
     8521 gaactgcctt cgcgcaggga caatatttgt actctgcaca gacaagattg agtaagagta
     8581 acttcaggaa taatggtgta catatcccct tgctgggtcg tattaaaggt tatctggctg
     8641 gaaagtgccg caaggagtaa taacgcattc atattcttct cccgaaaaaa aacagggctt
     8701 gcgccctgtt tctgtaatac aaatgatgta ttaatactga tgagcggtcg cgttgttacc
     8761 aaagccaacc tgagtcacgt tgacggagga gttcgatgca gtctggtcaa ctgcagcacc
     8821 gttgccacca ccgaactgtt taacagtcat ttcagaattt ttgccgttcc actgatcaag
     8881 agtggcgctg ttaccgaagc cacgttgggt cagatcgatt gagctgtcat ctgagccctg
     8941 accaacatct gcaccattac caccgccatg ctgggtaata gtcaagtcag agttacgggc
     9001 atcagtttgc agagcaagtg cagagttacc gccaccgtac tggtaaatgt tcagctctga
     9061 atttgggccg ctattattac tgaggtgtac tggcaatagc ggacactacc atttgttctt
     9121 ttttaagcag ccatctgatg atatttttcc ctgaaggctg ccggggagat
//
LOCUS       DAETQL010000140         8770 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.142, whole genome shotgun sequence.
ACCESSION   DAETQL010000140 DAETQL010000000
VERSION     DAETQL010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.142"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(80..871)
                     /gene="aadA1"
                     /locus_tag="K0W65_003759"
     CDS             complement(80..871)
                     /gene="aadA1"
                     /locus_tag="K0W65_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBI3096854.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(984..1814)
                     /locus_tag="K0W65_003760"
     CDS             complement(984..1814)
                     /locus_tag="K0W65_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HBI3096855.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            2024..3037
                     /gene="intI1"
                     /locus_tag="K0W65_003761"
     CDS             2024..3037
                     /gene="intI1"
                     /locus_tag="K0W65_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBI3096856.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3240..3590
                     /locus_tag="K0W65_003762"
     CDS             3240..3590
                     /locus_tag="K0W65_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBI3096857.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3716..4276
                     /locus_tag="K0W65_003763"
     CDS             3716..4276
                     /locus_tag="K0W65_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI3096858.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            4279..7244
                     /locus_tag="K0W65_003764"
                     /pseudo
     CDS             4279..7244
                     /locus_tag="K0W65_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
     gene            7311..7688
                     /locus_tag="K0W65_003765"
     CDS             7311..7688
                     /locus_tag="K0W65_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI3096859.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7889..8548)
                     /locus_tag="K0W65_003766"
     CDS             complement(7889..8548)
                     /locus_tag="K0W65_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HBI3096860.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1949 a         2480 c         2348 g         1993 t
ORIGIN      
        1 caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc
       61 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      121 gaacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      181 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      241 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      301 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
      361 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
      421 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
      481 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
      541 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
      601 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
      661 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
      721 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
      781 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
      841 tcgatacttc ggcgatcacc acttccctca tgatgtttaa cgtttgaaat aaggggcgcc
      901 gagcgccagc gaggggagcc aaaagcttgc ttttggccgt cccgacttga ttgaagggtt
      961 gggcgatttt gccattagat tttttataaa tttagtgtgt ttagaatggt gatcgcattt
     1021 ttcttggctt ttatgcttga tgttaaattc gaccccaagt ttcctgtaag tgcggacaca
     1081 aaaacatatt tatgtcctga tttgcttata ataaaccctt caaaccatcc gttttgtaag
     1141 gttctatttg ctgtgaatcc tgcaccagtt ttcccataca gttttgtact attatccaga
     1201 tcttgtagat acatgttctc tatggtgttt tctatggctg agtttttaac tgggagattg
     1261 tgattaataa ttttacgcag gaattgaatt tgttcttctg gtgaaatttt taagctactt
     1321 tcgagccatg cttctgttaa tccgttgttt ctttctttat ctccagagaa gtcttgattt
     1381 ccataatcaa aatctttgag ataattcttg attttattta atccaatttt ttgggttatt
     1441 tcttgcgaaa cccaaacaac agaaaattgc atccacgtct ttggtgtatg attgctgttc
     1501 cagatctcca ttcctttggg ggttttatcc catttgaata tggttttctg atctattatt
     1561 tccgcatcaa atgccataag tgataatgcg atcttgaaag ttgaatctgg tgccatttgc
     1621 gttgcacact ttgctttatt gaattgagca atttcagcgt ttgtggatgc atcgtaaagt
     1681 aaaaaacaac cttcagttcc ttcaaataat ggagatgcaa cagtagagat atctgttgat
     1741 gcactggcgc tgctgtagat aatatttgca attattaaaa aaatagcgaa gttgatatgt
     1801 attgtgtttt tcataataag tattggtttg gtaaagggct taattttaac ggctaacaat
     1861 taatgaggct ccgggttcgc ccaactttgt tttagggcga ctgccctgct gcgtaacatc
     1921 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc
     1981 ccgaggcata gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc
     2041 gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta
     2101 cttgcattac agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg
     2161 tttccacggt gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc
     2221 ctggctggcg aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt
     2281 gctgttcttc tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag
     2341 acctcggccg tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct
     2401 cggttttctg gaaggcgagc atcgtttgtt cgcccagctt ctgtatggaa cgggcatgcg
     2461 gatcagtgag ggtttgcaac tgcgggtcaa ggatctggat ttcgatcacg gcacgatcat
     2521 cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg ttacccgaga gcttggcacc
     2581 cagcctgcgc gagcagctgt cgcgtgcacg ggcatggtgg ctgaaggacc aggccgaggg
     2641 ccgcagcggc gttgcgcttc ccgacgccct tgagcggaag tatccgcgcg ccgggcattc
     2701 ctggccgtgg ttctgggttt ttgcgcagca cacgcattcg accgatccac ggagcggtgt
     2761 cgtgcgtcgc catcacatgt atgaccagac ctttcagcgc gccttcaaac gtgccgtaga
     2821 acaagcaggc atcacgaagc ccgccacacc gcacaccctc cgccactcgt tcgcgacggc
     2881 cttgctccgc agcggttacg acattcgaac cgtgcaggat ctgctcggcc attccgacgt
     2941 ctctacgacg atgatttaca cgcatgtgct gaaagttggc ggtgccggag tgcgctcacc
     3001 gcttgatgcg ctgccgcccc tcactagtga gaggtagggc agcgcaagtc aatcctggcg
     3061 gattcactac ccctgcgcga aggccatcgg tgccgcatcg aacggccggt tgcggaaagt
     3121 cctccctgcg tccgctgatg gccggcagca gcccgtcgtt gcctgatgga tccaacccct
     3181 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacaa
     3241 tggaggtggt agccgagggt gtggaaacac ccgactgcct tgcgtggttg cggcaggcgg
     3301 gttgcgacac ggtgcagggt ttcctgttcg ccaggccgat gccggcggcg gccttcgtcg
     3361 gcttcgtcaa ccaatggagg aacaccacca tgaacgccaa tgaaccgagc accagttgct
     3421 gcgtgtgctg caaggaaatc ccgctcgatg ccgccttcac gccggaaggg gccgagtacg
     3481 tggagcattt ctgcgggctg gagtgctatc agcgcttcca ggcgcgggcc agcactgcga
     3541 ccgaaaccag cgtcaaaccg gacgcttgtg attcgccgcc gtcaggttga ggcataccct
     3601 aacctgatgt cagatgccat gtgtaaattg cgtcaggata ggattgaatt ttgaatttat
     3661 tgacatatct cgttgaaggt catagagtct tccctgacat tttgcaggga attccatgac
     3721 tggacagcgc attgggtata tcagggtcag caccttcgac cagaacccgg aacggcaact
     3781 ggaaggcgtc aaggttgatc gcgcttttag cgacaaggca tccggcaagg atgtcaagcg
     3841 tccgcaactg gaagcgctga taagcttcgc ccgcaccggc gacaccgtgg tggtgcatag
     3901 catggatcgc ctggcgcgca atctcgatga tttgcgccgg atcgtgcaaa cgctgacaca
     3961 acgcggcgtg catatcgaat tcgtcaagga acacctcagt tttactggcg aagactctcc
     4021 gatggcgaac ctgatgctct cggtgatggg cgcgttcgcc gagttcgagc gcgccctgat
     4081 ccgcgagcgt cagcgcgagg gtattgcgct cgccaagcaa cgcggggctt accgtggcag
     4141 gaagaaatcc ctgtcgtctg agcgtattgc cgaactgcgc caacgtgtcg aggctggcga
     4201 gcaaaagacc aagcttgctc gtgaattcgg aatcagtcgc gaaaccctgt atcaatactt
     4261 gagaacggat cagtaaatat gccacgtcgt tccatcctgt ccgccgccga gcgggaaagc
     4321 ctgctggcgt tgccggactc caaggacgac ctgatccgac attacacatt caacgatacc
     4381 gacctctcga tcatccgaca gcggcgcggg ccagccaatc ggctgggctt cgcggtgcag
     4441 ctctgttacc tgcgctttcc cggcgtcatc ctgggcgtcg atgaactacc gttcccgccc
     4501 ttgttgaagc tggtcgccga ccagctcaag gtcggcgtcg aaagctggaa cgagtacggc
     4561 cagcgggagc agacccggcg cgagcacctg agcgagctgc aaaccgtgtt cggtttccgg
     4621 cccttcacca tgagccatta ccggcaggcc gtccagatgc tgaccgagct ggcgatgcaa
     4681 accgacaaag gcatcgtgct ggccagcgcc ttgatcgggc acctgcggcg gcagtcggtc
     4741 attctgcccg ccctcaacgc cgtcgagcgg gcgagtgccg aggcgatcac ccgtgctaac
     4801 cggcgcatct acgacgcctt ggccgaacca ctggcggacg cgcatcgccg ccgcctcgac
     4861 gatctgctca agcgccggga caacggcaag acgacctggt tggcttggtt gcgccagtct
     4921 ccggccaagc caaattcgcg gcatatgctg gaacacatcg aacgcctcaa ggcatggcag
     4981 gcactcgatc tgcctaccgg catcgagcgg ctggttcacc agaaccgcct gctcaagatt
     5041 gcccgcgagg gcggccagat gacacccgcc gacctggcca aattcgagcc gcaacggcgc
     5101 tacgccactc tcgtggcgct ggccaccgag ggcatggcca ccgtcaccga cgaaatcatc
     5161 gacctgcacg accgcatcct gggtaagctg tttaacgctg ccaagaataa gcatcagcag
     5221 cagttccagg cgtcaggcaa ggccatcaac gccaaggtac gtctgtacgg gcgcatcggt
     5281 caggcgctga tcgacgccaa gcaatcaggc cgcgatgcgt ttgccgccat cgaggccgtc
     5341 atgtcctggg attcctttgc cgagagcgtc accgaggcgc agaagctcgc gcaacccgat
     5401 gacttcgatt tcctgcatcg catcggcgag agctacgcca ccctgcgccg ctatgcaccg
     5461 gaattccttg ccgtgctcaa gctgcgggcc gcgcccgccg ccaaaaacgt gcttgatgcc
     5521 attgaggtgc tgcgcggcat gaacaccgac aacgcccgca gctgccagcc gatgcaccga
     5581 ccggcttcat caagccgcgc tggcagaaac tggtgatgac cgacgccggc atcgaccggc
     5641 gctactacga actgtgcgcg ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct
     5701 gggtgcaggg ttcacgccag ttcaaggact tcgaggacta cctggtaccg cccgagaagt
     5761 tcaccagcct caagcagtcc agcgaattgc cgctggccgt ggccaccgac tgcgaacaat
     5821 atctgcatga gcggctgacg ctgctggaag cacaacttgc caccgtcaac cgcatggcgg
     5881 cagccaacga cctgccggat gccatcatca ccgagtcggg cttgaagatc acgccgctgg
     5941 atgcggcggt gcccgacacc gcgcaggcgc tgatagacca gacagccatg gtcctgccgc
     6001 acgtcaagat caccgaactg ctgctcgaag tcgatgagtg gacgggcttc acccggcact
     6061 tcacgcactt gaaatcgggc gatctggcca aggacaagaa cctgttgttg accacgatcc
     6121 tggccgacgc gatcaacctg ggcctgacca agatggccga gtcctgcccc ggcacgacct
     6181 acgcgaagct cgcttggctg caagcctggc atacccgcga cgaaacgtac tcgacagcgt
     6241 tggctgaact ggtcaacgct cagtttcggc atccctttgc cgggcactgg ggcgatggca
     6301 ccacatcatc atcggacgga cagaatttcc gaaccgctag caaggcaaag agcacggggc
     6361 acatcaaccc aaaatatggc agcagcccag gacggacttt ctacacccac atctccgacc
     6421 aatacgcgcc attccacacc aaggtggtca atgtcggcct gcgcgactca acctacgtgc
     6481 tcgacggcct gctgtaccac gaatccgacc tgcggatcga ggagcactac accgacacgg
     6541 cgggcttcac cgatcacgtc ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc
     6601 gcatccgcga cctgggcgac accaagctct acatcccgaa gggcgatgcc gcctatgacg
     6661 cgctcaagcc gatgatcggc ggcacgctca acatcaagca cgtccgcgcc cattgggacg
     6721 aaatcctgcg gctggccacc tcgatcaagc agggcacggt gacggcctcg ctgatgctca
     6781 ggaaactcgg cagctacccg cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc
     6841 gcatcgagcg cacgctgttc atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg
     6901 tgcatgccgg gctgaacaag ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca
     6961 accgccttgg tgaaatccgt gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc
     7021 tcaacctggt gacggcggcc atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc
     7081 atgcgttgcg cggcaatggt catgccgtcg atgactcgct attgcagtac ctgtcgccac
     7141 tcggctggga gcacatcaac ctgaccggtg attacctatg gcgcagcagc gccaagatcg
     7201 gcgcggggaa gttcaggccg ctacggcctc tgcaaccggc ttagcgtgct ttattttccg
     7261 ttttctgaga cgacccctat tatggcgaag gtatcattca ggaagcgttg atgaaggatt
     7321 atctctgtaa aaagttattc aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg
     7381 gcaataacat tactggcctg gcatgttgca atattcttta tccctcgccc cgatacagcg
     7441 gtcagctgca tattaaagag ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta
     7501 aagcgataat gcgctttata gcccggcttg cgcttgaaca ggaatgcctt agccttagct
     7561 ggaacgctga aaaatccaac cccggcgcta accgttttta tcaggctctg ggaggcagaa
     7621 taaatgatca tatcgtcaat tattacctcc acggggagag cctgagcaaa ctggcctcag
     7681 gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt aaaccagcaa
     7741 tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc gaatttgctt
     7801 tcgaatttct gccattcatc cgcttattat cacttattca ggcgtagcaa ccaggcgttt
     7861 aagggcacca ataactgcct taaaaaaatt acgccccgcc ctgccactca tcgcagtact
     7921 gttgtaattc attaagcatt ctgccgacat ggaagccatc acaaacggca tgatgaacct
     7981 gaatcgccag cggcatcagc accttgtcgc cttgcgtata atatttgccc atggtgaaaa
     8041 cgggggcgaa gaagttgtcc atattggcca cgtttaaatc aaaactggtg aaactcaccc
     8101 agggattggc tgagacgaaa aacatattct caataaaccc tttagggaaa taggccaggt
     8161 tttcaccgta acacgccaca tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt
     8221 ggtattcact ccagagcgat gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag
     8281 ggtgaacact atcccatatc accagctcac cgtctttcat tgccatacgg aattccggat
     8341 gagcattcat caggcgggca agaatgtgaa taaaggccgg ataaaacttg tgcttatttt
     8401 tctttacggt ctttaaaaag gccgtaatat ccagctgaac ggtctggtta taggtacatt
     8461 gagcaactga ctgaaatgcc tcaaaatgtt ctttacgatg ccattgggat atatcaacgg
     8521 tggtatatcc agtgattttt ttctccattt tagcttcctt agctcctgaa aatctcgata
     8581 actcaaaaaa tacgcccggt agtgatctta tttcattatg gtgaaagttg gaacctctta
     8641 cgtgccgatc aacgtctcat tttcgccaaa agttggccca gggcttcccg gtatcaacag
     8701 ggacaccagg atttatttat tctgcgaagt gatcttccgt cacaggtatt tattcggcgc
     8761 aaagtgcgtc
//
LOCUS       DAETQL010000141         8765 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.143, whole genome shotgun sequence.
ACCESSION   DAETQL010000141 DAETQL010000000
VERSION     DAETQL010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.143"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            5..436
                     /locus_tag="K0W65_003767"
     CDS             5..436
                     /locus_tag="K0W65_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBI3096861.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="K0W65_003768"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(583..2745)
                     /gene="uvrD"
                     /locus_tag="K0W65_003768"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBI3096862.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="K0W65_003769"
     CDS             complement(2829..3545)
                     /gene="yigB"
                     /locus_tag="K0W65_003769"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBI3096863.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="K0W65_003770"
     CDS             complement(3545..4441)
                     /gene="xerC"
                     /locus_tag="K0W65_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBI3096864.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(4438..5145)
                     /locus_tag="K0W65_003771"
     CDS             complement(4438..5145)
                     /locus_tag="K0W65_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBI3096865.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="K0W65_003772"
     CDS             complement(5142..5966)
                     /gene="dapF"
                     /locus_tag="K0W65_003772"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBI3096866.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6003..6206)
                     /locus_tag="K0W65_003773"
     CDS             complement(6003..6206)
                     /locus_tag="K0W65_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBI3096867.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            6395..7873
                     /locus_tag="K0W65_003774"
                     /pseudo
     CDS             6395..7873
                     /locus_tag="K0W65_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7870..>8765
                     /locus_tag="K0W65_003775"
     CDS             7870..>8765
                     /locus_tag="K0W65_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBI3096868.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQ"
BASE COUNT         2034 a         2388 c         2252 g         2091 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgctttta aagccagtaa
      121 agatattcag tttgcggtgc agtcaggacc aatgttgatg gaaaacggtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt ggtggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagcggaaa ggttagaatg atagctttgt gtcagatact aaatatgctg ctgatgccgg
      481 atgcggcgta aacgccttat ccggcctaca tggcgttgca atttattgaa tctgaatgat
      541 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
      601 ggcgtaagct gccaccagcc atttgattcc ctggccctgg aatgccacct gcaaacggct
      661 atgttcaccg ctgccttcca tattgacgat ggtgccttca ccaaacttag cgtggcgtac
      721 acgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
      781 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcaa
      841 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
      901 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
      961 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca tgccctcttc
     1021 cataccgacg ataaacacct gcgggaactc cagacctttc gccgagtgca tggtcatcag
     1081 ttgtaccgca tcctgccagg tatccgcctg cccttcgcct gcttccagcg ccgcatggga
     1141 taggaacgcc tgcagcggca ttaaatcttc gtcttcttcg ttgtagctga actggcgcgt
     1201 tgccgtcacc agttcctcta agttttcgat acgcgtctga cctttttcgc ccttctcctg
     1261 ctcgtacatg gtacgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     1321 catatcggca gtttcctgcg ctaaggcgtc gatcagttcc ataaaccgct gtaaggcgct
     1381 ggcagcacgt ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     1441 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     1501 cgttggcgta ttcaccacac gctcaaaggc cgcgtcgtcg ttacggttgg caatcaggcg
     1561 cagatacgag agcgcatctt tgatttcctg gcgttcgaag aagcgcatcc cgccgtaaat
     1621 acggtacggc atactcgcct gcaacagtgc ttcttccagc acacgcgact gggcgttact
     1681 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     1741 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     1801 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     1861 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaataat
     1921 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     1981 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     2041 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     2101 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     2161 gcgcagcagc agctcggcga agtccaccag gcccgcgcga tcgcacgctt cctgatacgc
     2221 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     2281 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     2341 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgtagct ggtcttcgct
     2401 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     2461 gtgcgccagc ccgtggaagg tgcctaccca catgccgccc tggctggtgc ccatcagttg
     2521 tccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcacag ccataatcga
     2581 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     2641 cttaccactg cccgcgcccg ccagcaccag aaggttgctg cgtggcgcgg ccaccgcttc
     2701 gcgttgtttg tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta
     2761 aaaataggtt gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga
     2821 tttgctgatt atatcagcga ggtcagagat gccaaccggg aaatttccag atgtggcagt
     2881 aaacggctgt cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc
     2941 attccgctgc gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc
     3001 tcgccgatcg gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt
     3061 gagcgcccgt gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt
     3121 tccggctggg cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt
     3181 aaggtgtcgt gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc
     3241 attgctgcgt gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct
     3301 tgttcaatcg aacgaaaacg ccaacgtgtc acgtcgtgat aaatctcggg ttccgcttcc
     3361 cgtaccgcct ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga
     3421 taattttgca caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg
     3481 taaagggtat catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa
     3541 cgcattattt cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt
     3601 gaaaatcaag atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agtagctcct
     3661 gcacaccacg aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt
     3721 tatgtgggtg aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct
     3781 gcacattacg cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc
     3841 taccaaacag gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa
     3901 tcggcaggcg gcgctctttg ctgcctttcc ccatcaccca cacttcgccg gactccaggt
     3961 cgaggtgttt gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca
     4021 cttccagcat tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc
     4081 gattcatatc gtcgacgtcg atatttttcg gcagatgacg cggcgctttc ggtgccgaaa
     4141 ctcctttcgc cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac
     4201 gtagcgcaga aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta
     4261 cagcaaaatt gcgtaccatc gctgcatcgc attgttgcca gctttgcagg ccgttttcgc
     4321 tggcaaaatt gatgatcgcc tcaagctgac gctggtagtt aagtagggtt atcgggctaa
     4381 gctgacgctc cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca
     4441 tacgcgttca atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa
     4501 ctgcgttccc tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc
     4561 caaatcagca tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg
     4621 tagcaccacc agcagctccg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa
     4681 acgctgaata cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga
     4741 cggcgcacct aagcgccagc gatctggaaa caggcgcaga ctcgcaccag ctaggccgag
     4801 atcgcgcgcc cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag
     4861 actgcgctgc aggtagagta ggcgataaaa cagcccttcg ttggcgatag cctgctccat
     4921 caacaacgcc atgttctctt ccagaacatg aatgtgatta cgtgcgcggg ccatgtgcca
     4981 ctcgaccaac gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg
     5041 cgcattgcgg ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc
     5101 aagctccgtg agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatcca
     5161 tcgtagacat gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg
     5221 atatcaagac gaccgccggg gagttccacg cgtacttctt cgtccagcaa accttgctga
     5281 atccctaccg ccaccgccgc acacgcgccg ctgccgcagg cctgggtttc tcctgcccca
     5341 cgctcataaa cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg
     5401 gcgcgctccg gaaaacgctc gtgactttcc agaacaggac caagcgtttc taccgccgcg
     5461 gtatcgacat catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat
     5521 aagattgtct gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac
     5581 ggcacggcgg aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg
     5641 gtgacggtca gaaccatccg cccgttggcg gtgctgacgc ggatatcacg cttattggtc
     5701 agtcctttca gacgcacaaa acgggcaaag cagcgcgcac cgttaccgca ctgcgccact
     5761 tcactgccat cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc
     5821 tcaaccacca gcagctggtc aaaccctacc cccaggtgcc gatccgccag gcgacgaatc
     5881 agctccggtg aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca
     5941 aggccatgca ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac
     6001 ttttagtaat tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc
     6061 gtggattgcg tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc
     6121 gggaaataga gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aagtaataca
     6181 gtgagtgcct taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct
     6241 ttctatcatc gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga
     6301 aaagtgagga aatgctgcga gtataacgct tactcgtgag gatttatgtt ctgctggcag
     6361 tttcgtgatc aacacctcgc gccaggatgg ctttatgcac tatcgcaatg tggtttgttt
     6421 gttgtcttgt tcattgtttt tatcgtcggc atggggatgt cggctttccg cacctgaaca
     6481 taatatttac cagaaacagg gaaaaggcgt ggtgtacctg cgcccttata aagaaacaaa
     6541 tctttctcta cctgacgtga attacaagag tttgcgtcgg ttacccaatt tattaataga
     6601 cccgacaaca ctagacgaat gggataaaga accgcctctg actgatttga caaccgacta
     6661 cctctatgag ggcgcacagg cctggtatcc tcattattca tggcatagcg atggtcgcaa
     6721 tatcctctac gcgggtgaag ttgtgcaaaa ccctcccggc acacctcccg tcgatgtcgc
     6781 ctcgttcaag gcatggggtg attttgccgc agataaacac agtctctatt ttgaaggcaa
     6841 gcgcaccgat gacaatggtg gtggaaatag cttggatatc aaaacgctgc atcaggtgga
     6901 attccggcct ccctgggatc ctgacttact ggggctgata ttacgtgacg ctaatttctt
     6961 gtatatcaat gggcatcgcc tcgctgaccc cgagagtttc cgtgtgctgg cgcagaagtc
     7021 ctgggatcag cgaggaaagt tttccaccac gtttaacccc tgcattgccg taccatttgg
     7081 cccctgggat accctggctc gtacacggac caaaatcctg ctcaacggcg aacagcttga
     7141 tgccgacccg gacacctttt ccgtcgtgcg ctggatgccc ggctcactct tgacctggcg
     7201 tgataaaaac gggctgcagc gtaaagtcct cgacaaggaa aatctggcgt gggatgaaga
     7261 tttaacaaag cactgtctgg atttttctct gctggaaaag aaagtgttct ggcgtaaagg
     7321 acctgcttgt aaacaggaag aattacccgg actcgatccg gagcagtttc aacccatcag
     7381 tgatgctgtc gcccagcatc aggactcgct ttataccatc atcgaaacag agtctggtaa
     7441 ccgtaagctg gagatcgtga aacttgatga tcccaatctt attatcaaca aacgtttcaa
     7501 cgccgggaaa cgccacggct atttacttac gcgtgccgaa gggtagccat accattccgg
     7561 tttacacgtg tttgaatctg acggaccgct gatcttactg gataaccgtt ctccggatga
     7621 acgcgaagcc catcttaatg accatccctt tttgcgcaga tggtatgccc gcgataaccg
     7681 ctacatttac agctttgatg gcgcgcagct ctggcgatac cgcaccgctg atccgaaaca
     7741 agttcgctta atctggaagg aacaacattc gggatatgtc tatggcgtaa attacaaaac
     7801 gggatatctg gacggaaaaa ttactgatga cggcgaattt attcctgccc cgcgcaatga
     7861 ggcgacaaaa tgaagggtca aataatcaca gttgcactca tcctgttggg acacacattt
     7921 ctgacgcctg ctgtacaggc catagggttc gactattaca atgatcacgg tgttatgtct
     7981 tacggtaaag gttattgggg ggataagaaa atcattgccc agtttccgga gatgagcagg
     8041 gccggtttgc gccttgtgaa aaatatctct gggaaaacgg gttatatacc ttccgatgaa
     8101 ataagcatag aaaatgagga ataccactgg gtgacagacg gccgcgttat tctctggcgt
     8161 ggcaaaatcg ttagcaatcc accgggaaca ctcactgtcg atattgccag ctttcaggct
     8221 atgggccgct tcgcggtcga taaatatagc ctctattttg acggacagcg caccgaaagt
     8281 aatagcggcg cgtcacgtgt tgagctggcg acactaaaag ctatcgaagg taactctacc
     8341 acgctggtgg atagtaaaaa tctctacttg tccggtcgac gtcaggggag cagcagtgat
     8401 gttactgtat tagaaaaaag atggtggggt attaatccac gtcttatgag tgtaaacaga
     8461 aattcatatt ccaatgatct gcttatccgc agtgggcaga atatttattt aaatggcgtt
     8521 caccttacgg cgaatgcaga ctcatttgag ataattcgct ggatacctca ttcactgctg
     8581 gtttttcgcg acaataaggg tctgcatcgt tatccctttg gtcaattatc aggcaaagcg
     8641 atacccgtag atgatgacgt ctcttttgaa gtaggggaaa gtcgcgttcg ctggcgtaaa
     8701 cagctcacgc ccgaccgtca gtggagcaag tggatagacc taccaggtat tgaacctgaa
     8761 caatt
//
LOCUS       DAETQL010000142         8697 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.144, whole genome shotgun sequence.
ACCESSION   DAETQL010000142 DAETQL010000000
VERSION     DAETQL010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.144"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(813..2009)
                     /gene="hemY"
                     /locus_tag="K0W65_003776"
     CDS             complement(813..2009)
                     /gene="hemY"
                     /locus_tag="K0W65_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBI3096869.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2012..3235)
                     /gene="hemX"
                     /locus_tag="K0W65_003777"
     CDS             complement(2012..3235)
                     /gene="hemX"
                     /locus_tag="K0W65_003777"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBI3096870.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPQGDTPAAAPQGE"
     gene            complement(3257..3997)
                     /gene="hemD"
                     /locus_tag="K0W65_003778"
     CDS             complement(3257..3997)
                     /gene="hemD"
                     /locus_tag="K0W65_003778"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBI3096871.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3994..4935)
                     /gene="hemC"
                     /locus_tag="K0W65_003779"
     CDS             complement(3994..4935)
                     /gene="hemC"
                     /locus_tag="K0W65_003779"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBI3096872.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5322..7868
                     /gene="cyaA"
                     /locus_tag="K0W65_003780"
     CDS             5322..7868
                     /gene="cyaA"
                     /locus_tag="K0W65_003780"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBI3096873.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7908..8228)
                     /gene="cyaY"
                     /locus_tag="K0W65_003781"
     CDS             complement(7908..8228)
                     /gene="cyaY"
                     /locus_tag="K0W65_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBI3096874.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8276..>8697)
                     /locus_tag="K0W65_003782"
     CDS             complement(8276..>8697)
                     /locus_tag="K0W65_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HBI3096875.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1978 a         2243 c         2244 g         2232 t
ORIGIN      
        1 ttaagtgata ctggttgtct ggagattcag ggggccagtc tagcagttga cggggtatct
       61 gctgcgctgg ccatgagagg aagagcggcg gcgacagcaa caacaagacg tttgggtgaa
      121 aacgaaaatt ccatgcaaaa tgctccggtt tcatgtcatc aaaatgatga cgtaattaag
      181 cattgataat tgagatccct ctccctgaca ggatgattac ataaataata gtgacaaaaa
      241 taaattattt atttatccag aaaatgaatt ggaaaatcag gagagcgttt tcaatcctac
      301 ctctggcgca gttgatatgt aaggcaggtt tattatatcg cgttgattat tgatgctgtt
      361 tttagtttta acggcaatta atatatgtgt tattaattga atgaatttta tcattcataa
      421 taagtatgtg taggatcaag ctcaggttaa atattcactc aggaagttat tactcaggaa
      481 gcaaagagga ttacagaatt atctcataac aagtgttaag ggatgttatt tcccgattct
      541 ctgtggcata ataaacgagt agatgctcat tccatctctt atgttcgcct tagtgcctca
      601 taaactccgg aatgacgcag agccgtttac ggtgcttatc gtccactgac agatgtcgct
      661 tatgcctcat cagacaccat ggacacaacg ttgagtgaag cacccacttg ttgtcataca
      721 gacctgtttt aacgcctgct ccgtaataag agcaggcgtt tttttatgca tcagaaaggt
      781 cccggaggtg ctttcctccg ggtgagaagg aactactgtg gcgggttatt ctgcaacgtt
      841 aacatcaaac cgtcgcgacg catcgctgca gcttcttccg gcttgtgcag tctgtccagc
      901 gcgtcggcaa gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc cgcgcggaag
      961 gcgagcgatg cttcctgcca ttcaccgtgc ttcatcagtg actgacccaa tgtgctccac
     1021 aacagcgggc gatcaccgac gtttttgatt tgctggcgta gcactttttc cagctgttcc
     1081 gggttattgg ttttcagacg cgggatcggc agcagtaggc gatcgtcata ctggcgtttc
     1141 aggccgtcga tgataatttg ctgggcagta tcatgatcgt cacattcaat aagatgttcc
     1201 gccattgcca cctgcaacgc tacctgatga cgcgttttcc ggctttggtt tttccaccag
     1261 ttacgcaaac cttcactacc gttatcggca cgcgcctgat ccatcaggcc aatccatgcc
     1321 tgttgttcca gcattgcacg atgttcttca tcaccaacat gggctttcgc cattgatggg
     1381 atgatatcca gcagagaact ccatgcccct gtgcggatat acgcctgttc cgccagacgt
     1441 aatacttcag gatggcgtgg cgtaacttcc agcagcttat ccacgccgtg gcgtgcagca
     1501 tggttttcat tacgggccag ttgcagacgt acacgggtga tttctaccgg aatggtgtca
     1561 ttgccggcca gctccgctgc gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt
     1621 tgttgcgcgg cttcggcagc cagtagatag ttcaccaccg gttgttcagc gtgatcggca
     1681 tttttcgcca tcagcttttc aacttgctga taatcacctt ccgccagttt cagcagcgcc
     1741 tgttcggtct gcttacgcgc acggcgacgc ttacgtccga caaaccatcc acgggtgtgc
     1801 gcgccagtgc ggaagatccg ccgcagtagc cactcaatgg caaacagcac taccatcgcc
     1861 agaatcaata tgatcgccag gcccgtgacg ctggtttcga tattgtagtt gtcggtctgg
     1921 atcagcacat aaccctgatg accggcaatc atcgggccaa ccactatccc cgcaatcagc
     1981 aaaacaaaga gtaataacac ttttagcatg gttattctcc ttgcggcgcg gctgccggag
     2041 tatcaccttg cggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtgcag
     2101 gtgcaggttt agcttccgtt gtccccgctg ccggttgtgc cagcaggtta cgcacgcgag
     2161 tctgcatcag tttttccagc atcgcctggc tttgcagggt ttccggaaga tccatcgaga
     2221 tattttgctg gcttaactgg tccacctcgt cgaggaacgc tttggtggtg gcatcatcag
     2281 tatcgtagta agcacgtacc caggtggaga cgttctccag cgcctggcga taagtctctt
     2341 cctgatgacg cggtacagct tgtgctgcga ccagcaggcg agagcgaata ttttcgcgca
     2401 gatagatatc ctgatttggc gctaacagcg gtacggcggt gtcatcacga cggcgaatcg
     2461 taatgaagtt gtccataaag ttctgccagc ttttttgcag attgatacgc cattcgctga
     2521 tggaactgga aagctcttca ccgtctgaat ccatcggcga accatcgctg tcattatcgg
     2581 ccagacgcag gttatctacc tgatttgaaa gctgattaag cttaaggatg atgccgtcat
     2641 aatccacctg cgatactgca gaaaggctgg cgatatcatc ggtaattgcc cgacgaacgg
     2701 taatcagact cgggtcattc atatccgcca ggctggcgtc tgcacttttc agcaacgctg
     2761 cagaggtcgt gacgtcctga tcgctccaca gcttccgtcc ggcgagtttc accagaaaat
     2821 cggcctgagc cagcagccag gttttagcat cgctgccgga aatggtggcg atcttttgtt
     2881 ggacttcatc caactgtttt gccagcgttt cttgctgacg attcgcctgc ttaagctgcg
     2941 cagcttgttg cttaataatg ccttccagct cggctttttg gctctcctgg gctttttgca
     3001 atgccgtcag ttggttagcc agggcatcgc tggtggcggt ctgattgacg gcctgttgtt
     3061 taccccagcc atacaaaccg acacccgccg ccagagcaat agcgatagcc accgcgctga
     3121 gaatcaatgc agtattgttc ttactctttt tttctgttgc gacaggttgt gacgtggtgt
     3181 ccacggcctc cctggtctct tcaaccacgg cggaggtttt ttcttgttcc gtcattatgg
     3241 cttcctgtta tgagagttat tgtaatgccc gtaaaagcgc atcgttgtca gcgttatcgg
     3301 cgaccttaat gtcttgccag cccagttccc gggcgagttt cgccaaacgc tcactgacga
     3361 ccaatagtcg acagtgtagt aaccagtgct cacgatacca ttgtgggatc agcgaccaga
     3421 gttgctgcaa catttcaccg ctggtaacaa cgaccatcgt cacctcgcgg gattgccagc
     3481 gcatcgcttc ttctgcacca tcgtaatgga ttgcgcatcg ttgataacat tcacaaaaag
     3541 tgacctcagc accgcgcacc gtcagggtat ccccaattag ctcacgaccg ccattgccac
     3601 gtaatatcag cgcacgtttg cccgcaatat tttgtaattc aggtaattgt agcaagactt
     3661 cgctgatttc ccgatcctgt gggtagagaa tcttctgtcc gcttacggta tgtagcgcca
     3721 gtgcggtggt gcgtccaatg gcgaaataag taggtagttg gggccattta cgatcttgct
     3781 gatgcagctg tgattgggca aaagcaaccg cgtgttgcga gagggcaaac aacagatcgc
     3841 tctcccccag cgctgccagt tgatcagcaa gttgcggtaa ttgtcgaccc ggagaaaact
     3901 caatcagcgg aaaatgccag gccacctgcc ccagtgtgcg cagacggctc actaactctt
     3961 ctccagcggg agacgggcgg gtgaccagga tactcatgcc ggggcctctc cgttatagac
     4021 ttcagcgagg atctcgcgcg cgccgttatt cagtagctct tctgccagcg aaatccccat
     4081 ttgttcggca tcttgcggcg caccgcggcg ttcaccgcga ataatctgcg aaccgtccgg
     4141 cgcgccgacc agcgcacata gccagatttc gccatcaata agctcggcgt agctaccaat
     4201 tggcacctga catccgcctt cgagacgggt attcatggcg cgttctgcgg taacgcgcag
     4261 tgcagtttcg tggtgattca gcgcggcaag cagctcgcga gtgcgtgtat catcaaggcg
     4321 gcattcaata ccgactgcac cttgtcctac cgccggaaga gaaatctcgg gtggtaacgc
     4381 ggcgcgaatg cgggactcca gacctaaacg ttttagtccg gctacggcaa gaatgatggc
     4441 atcgtattcg ccgttatcta gtttgctcag gcgagtgccg acgttgccgc gcagggagcg
     4501 gataatcaga tccgggcggc gttcagccag ttggcactgg cgacgtaaac tggacgtccc
     4561 gacgatactg cctgctggta acgcatccag actgtcatag ttattggaca caaaggcatc
     4621 gcgaggatct tcacgctcgc aaatagtgac cagccccaga ccttgcggga attcaaccgg
     4681 cacatctttc attgaatgta cggcgatatc agcgcgattc tccaggagcg cgacttccag
     4741 ctcttttaca aataagcctt ttccgcctac tttcgccagc ggcgtatcaa gaatcacatc
     4801 gccgcgcgtc accatcggta ccagttcaac gaccaggccc ggatggctcg ccatcaactt
     4861 gtctttgaca tagtgtgcct gccagagtgc aagtgggctt tggcgtgtgg caattcttaa
     4921 aacattgtct aacatgcttg ttaccgtcat tatcatccgt ggtccatcct aacatccttg
     4981 ccagagtgat gtcagtgttg tggtgaaacg tagacgcctg cgcaaaccgt aaaatgaggt
     5041 ctggcagtgg atcctgacag gcgtttcacg ccgtcgtaat aaggaattta cagagaataa
     5101 acggtgctac acttgtatgt agcgcatctt tctttacggt caatcagcaa ggtgttaaat
     5161 tgatcacgtt ttagaccatt ttttcgtcgt gaaactaaaa aaaccaggcg cgaaaagtgg
     5221 taacggttac ctttgacata cgaaatatcc cgaatgccgc gtgttaccgt tgatgttggc
     5281 ggaatcacag tcatgacggg tagcaaatca ggcgatacgt cttgtacctc tatattgaga
     5341 ctctgaaaca gagactggat gccataaatc aattgcgtgt ggatcgcgcg cttgctgcta
     5401 tggggcctgc attccaacag gtctacagtc tactgccgac attgttgcac tatcaccatc
     5461 cgcgaatgcc gggttacctt gatggtaacg ttcccaaagg catttgcctt tacacgcctg
     5521 atgaaactca acgccactac ctgaacgagc ttgaactgta tcgtggaatg tcagtacagg
     5581 atccaccgaa aggtgagctt ccaattactg gtgtatacac catgggcagc acctcgtccg
     5641 tagggcaaag ttgttcctct gacctggata tctgggtctg tcatcaatcc tggctcgata
     5701 gcgaagagcg ccagttacta caacgtaaat gcagcctgct ggaaagctgg gccgcctcgc
     5761 tgggtgtgga agtcagcttc ttcctgattg atgaaaaccg cttccgtcat aatgaaagcg
     5821 gcagcctggg gggcgaagat tgtggctcca cccagcatat actgctgctt gacgaatttt
     5881 atcgtactgc cgtgcgtctc gccggtaagc gtattctgtg gaatatggtg ccgtgcgacg
     5941 aagaagagca ttacgacgac tatgtgatga cgctttacgc gcagggcgtg ctgacgccaa
     6001 atgaatggct ggatctcggt ggcttaagct cgctttctgc tgaagagtac tttggtgcca
     6061 gcctttggca gctctacaag agtatcgatt ccccatacaa agcggtgctg aaaacactgc
     6121 tgctggaagc ctattcctgg gaatacccga acccacgtct gctggcgaaa gatatcaaac
     6181 agcgtttgca cgacggcgag attgtatcgt ttggcctcga tccatactgc atgatgctgg
     6241 agcgtgttac tgaatacctg acggcgattg aagatttcac ccgtctggat ttagtacgtc
     6301 gctgcttcta tttaaaagtg tgcgaaaaac tcagccgtga acgcgcctgt gtaggctggc
     6361 gtcgcgcagt gttgagccag ttagtgagcg agtggggttg ggacgaagct cgtctggcaa
     6421 tgctcgataa ccgtgctaac tggaagattg atcaggtgcg tgaggcgcac aacgagctgc
     6481 tcgacgcgat gatgcagagc taccgtaatc tgatccgctt tgcgcgtcgc aataacctta
     6541 gcgtctccgc cagtccgcag gatatcggcg tactgacgcg taaactgtat gccgcgtttg
     6601 aagcattgcc aggtaaagtg acgctggtaa acccgcagat ttcacccgat ctctcggaac
     6661 cgaatctgac ctttatttat gtgccgccgg gccgggctaa ccgttcaggt tggtatctgt
     6721 ataaccgcgc gccaaatatt gagtcgatca tcagccatca gccgctggaa tataaccgtt
     6781 acctgaataa actggtggcg tgggcatggt ttaacggcct gctgacctcg cgcacccgtt
     6841 tgtatattaa aggtaacggc attgtcgatt tgcctaagtt gcaggagatg gtcgccgacg
     6901 tgtcgcacca tttcccgctg cgcttacctg caccgacacc gaaggcgctc tacagcccgt
     6961 gtgagattcg ccatctggcg attatcgtta acctggaata tgacccgaca gctgcgttcc
     7021 gcaatcaggt ggtgcatttt gatttccgta agctggatgt cttcagcttt ggcgagaatc
     7081 aaaattgcct ggtaggtagc gttgacctgc tgtaccgcaa ctcgtggaac gaagtgcgta
     7141 cgctgcactt caacggcgag caatcgatga tcgaagccct gaaaactatt ctcggcaaaa
     7201 tgcatcagga cgccgcaccg ccagatagcg tggaagtctt ctgttatagc cagcatctgc
     7261 gcggcttaat ccgtactcgc gtgcagcaac tggtttctga gtgtattgaa ctgcgccttt
     7321 ccagcacccg ccaggaaacc gggcgtttca aggcgctgcg cgtttctggt caaacctggg
     7381 gcttgttctt cgaacgcctg aatgtatcgg tacagaagct ggagaacgcc atcgagtttt
     7441 atggcgctat ttcgcataac aaactgcacg gcttgtcagt gcaggttgaa accaatcacg
     7501 tcaaattacc ggcggtggtg gacggctttg ccagcgaagg gatcatccag ttctttttcg
     7561 aagaaacgca agacgagaat ggctttaata tctacattct cgacgaaagc aaccgggttg
     7621 aggtgtatca ccactgcgaa ggcagcaaag aggagctggt acgtgacgtc agtcgcttct
     7681 actcgtcatc gcacgaccgc ttcacctacg gctcaagctt catcaacttc aacctgccgc
     7741 agttctatca gattgtgaag gttgatggtc gcgaacaggt gattccgttc cgaacaaaat
     7801 cgattggcaa cctgccgccc gccaatcagg atcacgatac gccgctatta cagcagtatt
     7861 tttcgtgatg agcgtgccgg aaagcgaggc ttatccggca tgcaatctta gcggaaactg
     7921 actgtttcac ccgcctgttg cgtcgccgcc tgttccagca aatcccagaa ggtttcgccg
     7981 ctgcgatcgc aaatccattc atcgcctttc aggtcaaaat ggtagccgcc ctgtttggtt
     8041 gccagccaca cctggtgcag cggctcctgg cggttaataa tgattttgct gccgttttca
     8101 aaggtaatgg tcagtacgcc gccgttgatt tcgcagtcga tatcgctgtc gccatccctg
     8161 tcgtccagac gctcttcaat ggtcagccac agttgatcag ccaggcgatg aaattcactg
     8221 tcgttcattg ttgtatcctg tttttaagtg atggcggcag tatagcggca tggggtcagg
     8281 gcttcaaagt ttgcacctct gctgctgcgt tccggcatga ttcatccgtc accggaataa
     8341 tgatgtctct gttttgaact tcacgtacgt tctcctctct gacacgagtt ttcgccgggc
     8401 agggggaggg cgtaatcctt acctctgtat cggtccatgt ataaacattg cgatcgtcat
     8461 aagcgaagcg atcgttttta gttagcgaac cgttaactgg aataatttgc aagctctttg
     8521 accgtaattg gcggataaag taagcatgct gtttgccgct attaaatgag cgatcaatga
     8581 ccaggtctgg cgtatccagc gggattatct caagctggtc ttcaccaaat gtcgataatt
     8641 ttgttacata cagccgatct ttatattgcg caatattgcc agtaatcaga tggaatt
//
LOCUS       DAETQL010000143         8375 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.145, whole genome shotgun sequence.
ACCESSION   DAETQL010000143 DAETQL010000000
VERSION     DAETQL010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.145"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..957
                     /gene="espL1"
                     /locus_tag="K0W65_003783"
                     /pseudo
     CDS             1..957
                     /gene="espL1"
                     /locus_tag="K0W65_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1129..1236
                     /locus_tag="K0W65_003784"
     CDS             1129..1236
                     /locus_tag="K0W65_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096876.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1289..2047)
                     /locus_tag="K0W65_003785"
     CDS             complement(1289..2047)
                     /locus_tag="K0W65_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HBI3096877.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2334..3263
                     /gene="pfkB"
                     /locus_tag="K0W65_003786"
     CDS             2334..3263
                     /gene="pfkB"
                     /locus_tag="K0W65_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBI3096878.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3364..3654
                     /gene="ghoS"
                     /locus_tag="K0W65_003787"
     CDS             3364..3654
                     /gene="ghoS"
                     /locus_tag="K0W65_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBI3096879.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3760..4620
                     /locus_tag="K0W65_003788"
     CDS             3760..4620
                     /locus_tag="K0W65_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBI3096880.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4661..5197)
                     /locus_tag="K0W65_003789"
     CDS             complement(4661..5197)
                     /locus_tag="K0W65_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HBI3096881.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5344..6012
                     /gene="hxpB"
                     /locus_tag="K0W65_003790"
     CDS             5344..6012
                     /gene="hxpB"
                     /locus_tag="K0W65_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HBI3096882.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6175..6765
                     /locus_tag="K0W65_003791"
     CDS             6175..6765
                     /locus_tag="K0W65_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBI3096883.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6898..8288
                     /gene="tcyP"
                     /locus_tag="K0W65_003792"
                     /pseudo
     CDS             6898..8288
                     /gene="tcyP"
                     /locus_tag="K0W65_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT         2175 a         2004 c         2006 g         2190 t
ORIGIN      
        1 atgctcagac ctgggctgat aggtatgttc gcaactgtgc aattgctatt ctgcccctat
       61 tacctgtggg agttctaggg gctattttcg cgggtattgc atcacgaatg ccatttgccc
      121 ctatacatcc atcggcaatg ttgttaataa tggctacagg ccagactcaa cagcttatta
      181 cattattcaa acagttgccc atactccctg aaaaagaaat cattgaaata ataactgcgc
      241 agaatagcgt tggtacacct gctttatttt tggctatgat gaacggacat actgacaacg
      301 tgaaaatatt tatgcaagaa attcagtcac tggtagataa tcacatcatt catgaagata
      361 atctggttaa attactgcaa actaaaagtg ctaacgaaac acctggactt tatatctcca
      421 tgttgtatgg attcgatgaa ataatcgata tctttctgaa tacattaacc actccaatag
      481 cactaagagc ttttaaacaa aaaactggtg atgagtattt tagccataaa aatacatgat
      541 ggtgagccag gattatacgc cgcaatggaa aataatcacc ctttgtgtgt cacacggttc
      601 ctctctaaaa ttaatggcat cgcctttaaa tacaagttga gcaaagctaa catcatggat
      661 ttattaaaag gagctacagc acagggaacc cctgcattat acatcgccat gagcaagggt
      721 aatgaagacg tcgtgttatc ttatatatcg acgctgggtg cttttgcaaa aaaacattct
      781 tttagtcaac atcagttatt tacactattg gccgctaaaa atcatgacaa catgtcagct
      841 gttcatatag ccattcatca taagcattat aaaactgtag aaacatatta tgctgctata
      901 aatgtcatca gccaaagcct gagttttagt gctgatgaaa taaagacgta tttataacca
      961 gctatttacg atatatatcg atacataatt ttattttttt cataaaacgc agaaacagtg
     1021 accatgtgca caacgccacc cgacggctgt aataacaaaa aatcccttga tgcctgtccc
     1081 ttttgttaca ctccgttatc acgcacaaga gatatgcagg acactggtat gccgactaaa
     1141 cgctttgata aaaaacactg gaagatggtg gtggtgctac tggcaatctg tggcgctatg
     1201 ttgttgctac gttgggcagc aatgatttgg ggctgagcag tgacaaacga agacagccag
     1261 accgcataac acggtccggc agataatttt acatgctgta gcccagcgat aaggttgtcc
     1321 gacggtcggt atgttccggt gcagactctg gaggttcaga gttccatgtg acgttgtaag
     1381 ccaccttcag tccaaaatgt tcattaatag caacatttaa tgcgctttcg gagttcagcg
     1441 ttgtgtcttc cgcgccaaag accgaaacac cctgcgtaaa tttagcgttg tcagtcaact
     1501 gccatgcata agccccggag gcatagccca gcggctgggt ttcactggca ttgtcggtat
     1561 atttgtcgta acgcacacct ggaccgaatt caaagcggaa actgtgtacc gggccattca
     1621 aaaactgacg accataaccc gcggtcaaca catcgcgctc tcgatagccg ttataacggt
     1681 ctgtcagcca gcttgcctga ccaaataaat agtcataatc agttaaatta aagcggctac
     1741 gcccgcccgc cgcatatttt tctgaagaac gctcatcatt agaagaagta ttactggcgt
     1801 tcccccacag cgaccaggca gtggtttgtc cataccaggt catggtggtg tcagccgtaa
     1861 gtgaggagct tttcgtattg cctgattgtg caagatatcc tgcgttcaga ttaccttcga
     1921 aaggtttttt agcgctggca ggatcgtcca tgacagtaaa aacggaatca tcggcagctg
     1981 cattcagtga cgcaaacatg ccccccgcca acataacgat ggcaggaact gtcttcaaaa
     2041 gcttcattta tcaagagtcc gtacaacaaa aaaagagacc atcgcggtcc cggaaacttt
     2101 cttaaggatc aaagattagc gtccctggaa aggtaacgaa ttataaaaag gcgcgaataa
     2161 cttagcaatg tattcttatt tcattttttg aataagcatg tggcgaaaac agatttttat
     2221 ttatatatat ttatctgcaa aattttaaat aaagctctaa taaatcatat tgttaatttc
     2281 ttcactttcc gctgattcgg tgccagactg aaatcagcct ataggaggaa atgatggtac
     2341 gtatctatac gttgacactt gcgccctctc tcgatagcgc aacaattacc ccgcaaattt
     2401 atcccgaagg aaaactgcgc tgtaccgcac cggtgttcga acccgggggc ggcggcatca
     2461 acgtcgcccg cgccattgcc catcttggag gcagtgccac agcgatcttc ccggcgggtg
     2521 gcgcgaccgg cgaacacctg gtttcactgt tggcggatga aaatgtcccc gtcgctactg
     2581 tagaagccaa agactggacc cggcagaatt tacacgtaca tgtggaagca agcggtgaac
     2641 agtatcgttt tgttatgcca ggcgcggcat taaatgaaga tgagtttcgc cagcttgaag
     2701 agcaagttct ggaaattgaa tccggggcca tcctggtcat aagcggaagc ctgccgccag
     2761 gtgtgaagct ggaaaaatta acccaactga tttccgctgc gcaaaaccaa gggatccgct
     2821 gcatcatcga cagttctggc gaagcgttaa gtgcagcact ggcgattggt aacatcgagt
     2881 tggttaagcc taaccaaaaa gaactcagtg cgctggtgaa tcgcgaactc acccagccag
     2941 acgatgtccg caaagccgcg caggaaatcg ttaatagcgg caaggccaaa cgggttgtcg
     3001 tttcccttgg tccacaagga gcgctgggtg tcgatagtga aaactgcatt caggtggtgc
     3061 caccaccggt gaaaagccag agtaccgttg gcgctggtga cagcatggtc ggcgcgatga
     3121 cactgaaact ggcagaaaat gcctctcttg aagagatggt ccgttttggc gtagctgcgg
     3181 ggagtgcagc cacactcaat cagggaacac gtctgtgctc ccatgacgat acgcaaaaaa
     3241 tttacgctta cctttcccgc taacaaaaac attcccccag cattggggga atcaccacca
     3301 acctgtcggc aacgcgtttc tccgactatg ctcaaaagtc atgtgataac aaaggggtga
     3361 actatggcca gtggcgatct tgtccattat gtcataaccg tcatgttgca tgaggataca
     3421 ttgactgaaa ttaacgagtt gaataattac ctgactcgcg acggtttttt gctcaccatg
     3481 acggatgatg agggaaatat ccatgagctg gggactaaca cttttggact tatcagtacc
     3541 caaagtgaag aagaaattag agaactggtt tcggggctta cccaaagtgc aaccggcaaa
     3601 gatcctgaaa tcaccatcac gacctgggag gaatggaata gcaacagaaa ataaatggtt
     3661 tttgggcaat aatcagtctg tggtgtgcgt tagctcgtgt ttttacaccg cattcttgcg
     3721 ctaaccttat gatctggcag acaacatggg agagacatca tgtggcaggc aatcagtcgt
     3781 cttttgagcg agcagttagg tgaaggcgaa atcgaactgc gtaatgaact gcctggcgga
     3841 gaagtccatg ccgcatggca tttgcgctat gcaggacatg actttttcgt caaatgtgat
     3901 gaaagggaac tgcttcccgg ttttaccgcc gaagccgacc aactggagtt actgtcgcgt
     3961 agtaaaaccg tcaccgtgcc taaggtttgg gcagtaggcg ctgaccgtga ctacagtttt
     4021 ctggtgatgg attatctccc acctcgtccg ctggatgcgc atagcgcatt tattcttggt
     4081 cagcaaattg cgcgtttaca tcaatggagt gaccaaccac aatttggcct cgattttgat
     4141 aactcgctct ccacaactcc acagcccaac acctggcaac gtcgctggtc aacgtttttt
     4201 gctgaacaac ggattggctg gcagttggaa ctggcagcag agaaagggat cgctttcggc
     4261 aatatcgacg ccatcgtcga gcatattcag cagcgtctgg cctcgcatca accgcagcct
     4321 tctctgttgc acggcgattt atggtccggc aactgtgcgc tgggtccgga tggcccgtac
     4381 attttcgacc ctgcctgcga ctggggtgac cgagagtgcg acctagcgat gttaccgctg
     4441 catactgaac agccgccaca aatctatgac ggctatcagt cagtatcccc gctacctgcc
     4501 gatttccttg aacgtcaacc ggtttaccaa ctctacacgc tgttaaatcg tgcaagatta
     4561 tttggcggtc agcatttggt tattgctcag cagtcattgg atagattatt agcagcatga
     4621 tatgggttga ggataatggc cgctccgtgc ggccttttga ttaaataaac ccaagcagag
     4681 aaaagaacac ataacctgca gcaatacaaa taaccggcag gatatatagt gagaaaaact
     4741 gcaggaaaat agtatggtgc ggaacaacaa tacgggactc aatttgttca cgcgttagcc
     4801 cctcttcccc tttggctttt tccagaatga gttgatcttc aacaccttcc cgcaagaagc
     4861 gcgcctggcg actcatccgg gcaccggaat cctgcaacgc cagtccgaca aaaatcagga
     4921 tgaaaatcac ccagaacata acgttcacac caccattaaa attaggggtc ggggagttat
     4981 accagaacag attcaaaaaa ggcgtattcg cctgcatcat atcgatcatg acatgagcaa
     5041 agtcgagcat gaccgcatta atgccttcct ggttttcctg ccgggtattc ataaatttca
     5101 gcaaagaaat caatgtagag atcaatgcag gtataaaaat cacccaaccc aaaatccttt
     5161 tcaaaacagc aatgcgtcca gcttgttgat acgtcatgag ttctccttga ttaagacgcg
     5221 tcgtttcact tagtttacct gtagatatct gttttcgccc attctttaaa ggcgatatga
     5281 taggcgctta atcataagca cggcttaata ccttacacat aatgctctaa aggagaggtt
     5341 gtaatgtcaa ccccgcgtca gattcttgct gcaatttttg atatggatgg attacttatc
     5401 gactcagaac ctttatggga tcgagccgaa ctggatgtga tggcaagcct gggggtagat
     5461 atctcccgtc gtaacgagct gccggacacc ttaggtttac gcatcgatat ggtggtcgat
     5521 ctttggtacg cccggcaacc gtggaatggg ccaagccgtc aggaagtagt agaacgggtt
     5581 attgcccgtg ccatttcact ggttgaagag acacgtccat tattaccagg cgtgcgcgaa
     5641 gctgttgcgt tatgcaaaga acaaggttta ttggtgggac tggcctccgc gtcaccacta
     5701 catatgctgg aaaaagtgtt gaccatgttt gacttacgcg acagtttcga tgccctcgcc
     5761 tcggctgaaa aactgcctta cagcaagccg catccgcaag tatatctcga ctgcgcagca
     5821 aaactgggcg ttgacccact gacctgcgtg gcgctggaag actcggtaaa tggcatgatc
     5881 gcttctaaag cagcccgcat gcgttccatc gtcgttcctg cgccagaagc gcaaaatgat
     5941 ccacgttttg tattagcaaa cgtcaaactc tcatcgctga cagaactcac cgcaaaagac
     6001 cttctcggtt aatgaccagg ggcagtgatc acgctgcccc tggttcttta tctgaatttg
     6061 cattcaactg acggattaat cgtcaattta agagaaagag ttacaccgtc accacttccg
     6121 tgcactgtat aaaaatccta tactgtacgt atcgacagtt tagtgagttt tatcatgacg
     6181 gcggaaggtc accttctctt ttctattgct tgtgcggtat ttgccaaaaa tgccgagctg
     6241 acgcccgtgc tggcacaggg tgactggtgg catattgtcc cttccgcaat cctgacgtgt
     6301 ttgttaccgg acatcgatca cccaaagtcg tttcttgggc agcgattaaa atggatatca
     6361 aaaccgatcg cccgcgcttt tgggcatcgt ggttttaccc acagtctgct ggcggtattt
     6421 gcgctgctgg caacctttta ccttaaggtt ccggaaggct ggttcattcc ggctgatgcg
     6481 ctacaaggaa tggtgctggg ttatttgagc cacatacttg ccgatatgct gacacccgcc
     6541 ggtgttcccc tgctctggcc atgccgctgg cgtttccgct tgcctatcct ggttccccaa
     6601 aagggcaacc aactggaacg ttttatctgc atggcattat ttgtctggtc ggtatggatg
     6661 ccccattcat tacccgagaa cagcgctgtt cgttggtcat cgcaaatgat caataccttg
     6721 cagatccagt ttcatcggct tattaagcat caggttgaat actaaaaagg caaaaatcac
     6781 ctttctggaa taagcaattc catttgaata taagagccag ctcacagttc tgttaatctt
     6841 gcgccaacac tatgactgct acgcagtgat agaaataata agatcaggag aacggggatg
     6901 aactttccat taattgcgaa catcgtggtg ttcgttgtac tgctgtttgc gctggctcag
     6961 acccgccata aacagtggag tctggcgaaa aaagtgctgg tgggtctggt gatgggtgtg
     7021 gtttttggcc ttgccctgca taccatttat ggttctgaca gccaggtact taaagattct
     7081 gtacagtggt ttaacatcgt tggtaacggc tatgttcaac tgctgcaaat gatcgttatg
     7141 ccgttagtct tcgcctctat tctgagcgcg gttgcccgtc tgcataacgc atctcagtta
     7201 ggcaaaatca gttttctgac catcggtacg cttttgttta ccacgctgat tgcggcgctg
     7261 gtcggtgtgc tggtcaccaa cctgtttggt ttgacggctg aaggtctggt tcagggtggt
     7321 gcagaaactg cacgtctgaa cgccattgaa agtaactatg ttggtaaagt ctctgatcag
     7381 agcgttccgc agctggtctt gtcctttatc ccgaaaaacc cgtttgccga tcttaccgga
     7441 gccaatccga cgtcaattat cagcgtggta atttttgccg cattcctcgg cgtagctgca
     7501 ttgaaactgc tgaaggatga tgcgccgaaa ggggaacgcg tcttaaccgc tatcgatacc
     7561 ctacaaagct gggtgatgaa actggttcgc cttgtcatgc agttgacccc ttacggcgtt
     7621 ctggctctaa tgaccaaagt ggttgcaggt tctaacttgc aagacatcat caaactggga
     7681 agtttcgttg tcgcgtccta cctcggtctg ctgattatgt ttgctgtgca tggcattctg
     7741 ctgggcatta atggcgtgag tccgctgaag tacttccgta aggtatggcc tgtgctgacg
     7801 tttgccttta ccagccgttc cagtgctgcg tctatcccac tgaatgtgga agcacaaacg
     7861 cgtcgtctgg gcgttcctga atccatcgcc agtttcgccg cctctttcgg tgcaaccatt
     7921 ggtcagaacg gctgcgccgg tttgtatccg gcaatgctgc ggtgatggtt gcgcctacgg
     7981 ttggcattaa cccgctggac ccgatgtgga ttgcgacgct ggtcggtatt gttaccgtta
     8041 gttccgcagg cgttgccggt gtcggtggtg gtgcaacttt cgccgcactg attgtgctgc
     8101 ctgcgatggg cctgccagta accctggtgg cgctgttaat ctccgttgaa ccgcttatcg
     8161 acatgggccg tacggcgcta aacgttagtg gctcgatgac agctggcacg ctgaccagcc
     8221 agtggctgaa gcaaaccgat aaagccattc tggatagcga agacgacgcc gaactggcac
     8281 accgttaatt cttatgctgg caacggtccg ttttgtatag gggccgttgc tttacttttt
     8341 aaatgttcca tcgcggcgat gcatactgac tcata
//
LOCUS       DAETQL010000144         8280 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.146, whole genome shotgun sequence.
ACCESSION   DAETQL010000144 DAETQL010000000
VERSION     DAETQL010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.146"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            8..658
                     /locus_tag="K0W65_003793"
     CDS             8..658
                     /locus_tag="K0W65_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096884.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            630..1064
                     /locus_tag="K0W65_003794"
     CDS             630..1064
                     /locus_tag="K0W65_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBI3096885.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1837..2886)
                     /locus_tag="K0W65_003795"
     CDS             complement(1837..2886)
                     /locus_tag="K0W65_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBI3096886.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINAINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3337..4527
                     /locus_tag="K0W65_003796"
     CDS             3337..4527
                     /locus_tag="K0W65_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HBI3096887.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4917..6377)
                     /gene="gtrII"
                     /locus_tag="K0W65_003797"
     CDS             complement(4917..6377)
                     /gene="gtrII"
                     /locus_tag="K0W65_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HBI3096888.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6374..7303)
                     /locus_tag="K0W65_003798"
     CDS             complement(6374..7303)
                     /locus_tag="K0W65_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBI3096889.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(7300..7662)
                     /locus_tag="K0W65_003799"
     CDS             complement(7300..7662)
                     /locus_tag="K0W65_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBI3096890.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
     gene            complement(7924..>8280)
                     /locus_tag="K0W65_003800"
     CDS             complement(7924..>8280)
                     /locus_tag="K0W65_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBI3096891.1"
                     /translation="VFVHTKPAYRSDGTKTAAVRKMRTDSNKAWKGALKRAGISNFRF
                     HDLRHTWASWLVQSGVSLLALKEMGGWETLEMVQRYAHLSAGHLTEHASKIDAIISRN
                     GTNTAQEENVVYLNAR"
BASE COUNT         2531 a         1598 c         1686 g         2465 t
ORIGIN      
        1 gtaatccatg catcgtatag acacgaaaac cgcgaagaag gataagttcg gcgcgggtaa
       61 gaacggtttt acccgtggta acccccagac tggcacgcct gccaccgatc tggatgatga
      121 ctactttgac atgttgcagg aggagctttg cagcgtggtg gaggcatccg gtgccagcct
      181 ggagaagggg cggcacgatc agttgcttac cgcacttcgc gcgctgctgt taagccgcaa
      241 gaatccgttt ggcgatatca aatcggatgg cacggtgaaa acggctctcg aaaaccttgg
      301 tttgggagaa gcagctaaaa ggaatgtagg tacaggggcg aatcagatac ctgatatgag
      361 cctgttcgcg tcaattaata ccgtaacggc tgctgcgcaa aaatttccgt ctggattaat
      421 tttacagtgc ggtcagttga atggtgcccc gaatgtatct tcaacatacg ggatgaggtt
      481 cccgatgacg ttctcaagag taattgctgt cgtagttaca ttgaacgtta ctggcgcggc
      541 tgggcagccg actgtatcgg cgacaagtgt ccagaacact ggatttaata ttactgtgtc
      601 gcccggttca ggatacggtt catctgctga tgcgtattac attgcaatgg gatattaaca
      661 aaatgtcata tttttattct gcatcgacaa acggatttta ttcgactgaa tttcacggca
      721 ccaatattcc tgatgatgca gtggaaatct cggaatcaga gtggaaaaca ctgattaatg
      781 cacagagtgt aacaaaaatg attacctgtg gtgagaacgg tcatcctgtc attgttgacc
      841 gtccttctcc aacaccagaa caattagcct taataaatga tgaaaagaaa tctgcactga
      901 tagcagaggc aacgaatgta atagctccgc ttcaggatgc ggttgattta ggtatggcaa
      961 cagatgatga aacgaaactg ttactggcat ggaaaaaata tcgggtgctg cttatgcgtg
     1021 ttaatgtagt aaaacccgag tggcctatgc atccaaataa atagacttgt taatctagga
     1081 taattgttgg aataatactc gtttttcagt aggttcaggt agcctgggta taatgctcac
     1141 ttctacaatg atttcaactt attttattta attgatatta cgaaacttaa ctcatacata
     1201 aacaatatga ctcaatactg tcctaaatag ctgcgcagaa aagtgctcca ctttcgctca
     1261 gcccttgctg ggcgaagcat caggaaggcg ttctcagcta ggttgtgtta tatctggctt
     1321 gagttttgtt gcccatacgc aacacattac ccatctgtaa acgacaagtg tggcagaatt
     1381 aggtgcagct atttagacta tatcgacgca gtaacgataa tagatatcga aactgtacta
     1441 gatattagct acttatggtt accgggggga gtcttttgag cactggtgtt atcaattggc
     1501 ttcaaataat tcgccaataa attatattgc gatagctcat caccttccag gttgtttata
     1561 gccagtttat cagcgtcacc acctactgtt tatgctttta tttctttgat taataagtat
     1621 atgatatttt aattcatctg gtgtatttct ttctcatgtg ttggtcgact gaatatctgt
     1681 tcagtgaagg ggtaatttta tgtttgcact tcgaacattc ccttcagttg ctaggaaaga
     1741 aaaccgtaac atttgcgcat taattttaac agtataatct gagcataaat atgcgctata
     1801 tatctataaa aaggcgcaat taattgcgcc tttttatcat tttatcaaaa atggcccacc
     1861 gactgtcctc gatagccaat ggccaatttt atctgataca tatacacaaa tacaggtata
     1921 tatgattgcg cagataggat ttggaatagt cacgctgaag cctataattg ctaaagccat
     1981 agggataaat aatttggtga tatactccat tccacatatg ttaagagtat ttcttccagc
     2041 tcttacaata aagtcatttc cacaaattat ttttgctatg aaaatattaa agattatcaa
     2101 gcctaccgtg gtgataatag tattaatagc ttgaatataa ctacttgata ttatgtctgc
     2161 attgaatatt gagataatct taaaaagtaa aggtttctgg ttaagtagtt gatacgcact
     2221 cattatagcg gtaattgaga agacaatgaa accaatttta gattttccaa aaaacctgtc
     2281 acgtgttaat tctaaaaaca tacatctacc gagaggcaat aaccaccagt atgccatagc
     2341 ggagtctatg ttccaaaacc attgaggatc tagtaatggg ttatgattga gcactgtttg
     2401 tgacaacatg aacgataaca gtgatatgat taaaatcact atattgttct tgactatttc
     2461 tctaaagatt gcatcaattg ctattataac aaaaaggcag tttataaacc atattgttcc
     2521 tacgaattgg ttatttctaa caccatatat tatatcataa atgtggttgt atatataatc
     2581 aatcgtctca cctgagttaa ttgcgtttat tattaatata gaaaatgcaa aagtgaaata
     2641 tggaatcatt aatctgtaaa actttgactt gatataatcg aaaacagata agtcattttt
     2701 tttgatagta aaaaaaccag cagcgaaaaa gaatagtggg acgtgataac taaaaacaaa
     2761 aggatacaat ttgccagccc ccaacccaag gtgacctaaa tatatagcaa aaataccgag
     2821 aaattttaaa gcatcaaccc aatcatgtct tatttttctg ttgctgtcga ttttgttatt
     2881 atacatttaa agtaggctct ccagttttgt gtgattttta aacactcatt gcaaatatga
     2941 atgcctgaaa gtttatgatt attaatcaac gagttcaata ttaatttatt atttttcaaa
     3001 gtcgcactat ataggcattt acttccgcag taccaccgat agtatcgata tcaaatcagc
     3061 ctgcggccgc gataactttc gtaacattct gccagcggct ttgttgaata aatcaggttc
     3121 aagataatcg tctctgcatc aggttagagt ttcaggcaat acatcctctt tctggcatac
     3181 ctgccactct cgcaccatgg tcatagcctt tacgatctgc atactcacca gaccagagcc
     3241 cgctcccttt taggctgagg aatccccaga aaagaagaca ggcatagagt tttgtgatct
     3301 actgattgcg ctaacaaatt caatgagatc acctctatgc ctgcccgtaa agtatgccag
     3361 acttttttcc gtaatgcttt agcttccacg catcagtatc gacaaaatgc cattattgat
     3421 tctgctgctg ctttagtcgg tggcgcatct ctttctctta ccagcattgg ccggcattta
     3481 cccggtcctg ctcgcgtgaa agataaaata aaacgcgttg actgtctgtt gggtaataaa
     3541 cgacttcata atgatatccc tctgatattc aaaaatatta catccatgat gacaaataaa
     3601 ctgtcgtggt gtgttatcgc tgttgactgg agcggttacc cttttcagga atatcatgtc
     3661 cttcgtgcca gcctcctctg cgacggtcgc tctatccccc tgatgagtca ggtcttcccg
     3721 tcaaaaaaaa ataacgaggc ggtggaaatc gcttttctgg atgcgctttc cggggccatt
     3781 tctccccgga ctcgtgttgt tattgttact gatgctggtt ttcaaagtgc atggttccgt
     3841 catatcaaat cacagggatg ggattttatt ggtcgtatca ggggggtagt gaagttcagg
     3901 ctggatagtg acaaagataa gtggctggac ataaaaatgt gcagggggag ttcagaggca
     3961 aaatacctgg ggacgggaac cctggcccgg aaaaaacgct cgcagtgtga aggacatttt
     4021 tatctttata aacattcacc taaaggcaga aaaagcaggc gtgccagagg gaggccgggc
     4081 ttaccaacaa cggaaaaaga acaaaaagcc gcaggcagag aaccatggtt aatattcagc
     4141 aatacagctg agtttaatgc caaaaaaatc atgaaactgt acagtcgaag aatgcagata
     4201 gagcaaaact ttcgggatga aaaaagtgaa cgctttggtt ttggccttcg ggcgagcaga
     4261 agtcggaggg gagagcgttt tctggtatta agtctactgg ttacactggc cagtatcgta
     4321 ctctggctac ttggttatca ttttgaaaat aaaggatttc atttaaagta tcaggctaat
     4381 agtctcaaaa aacgaagagt attgtctttt ctgacgctgg ctgaaaacgt cctgagattt
     4441 aaccctgaac ttctacgacg agcacaacct gaaaagatat tgtgccggtt ggccagcact
     4501 taccgaagta tggtgctggc ttactgatga aaattttctg gggatccctc agcctttcgg
     4561 ggaggaatga gggctctgat attcttttgc cgtagttcat cgtgaaaccg tcggatgtca
     4621 tttggcctga gtgacccatg cctcccaaaa gcagagtcgc tagtgttatg taattaggcg
     4681 gttaaacttc aattctcagt gttaagtttg ggtaaacgct tgtaaatggt attgctaatt
     4741 ttttaattga ctaatattag aaacggttag tgtgattgta tatagatatt tgagtgtaag
     4801 gtatttaaag gggcattcag cccctccgtc tatttgtgat tgcatccatt gatgtgtgta
     4861 ttattttgag cttgaaaaag ggaggcgctc tttaataata aataaatcta tcaaagctaa
     4921 atattaaatg gaagccaccc ttttttctct gatagtttta ttatcaccaa accatttatt
     4981 tcagctatcg aatctggatt cggccaggaa ggtaaactct tcacaattgg ataaatcttc
     5041 tctatttgtt ctttatttgc tggtttgcaa ttacagattc catagtaatt cataaatgca
     5101 accatcctaa aatagttgcc accatcccac caaaaaaaag attttccaaa agtgtctgcg
     5161 ctcatggaga gcatatttcc tgagtcgttt gaaccatata tatatatata ttttccgtta
     5221 agggaaatgc cttttgtttg agatgtgtat gatatttctt tagctaaaga gatatctttc
     5281 tgtcttgcga gagtatcatt caaaaataga tttttggatg ttgaaacgcc attaaatata
     5341 ataaataaaa aaaacatgca gtttaggtat tttatagagc gaaaattgct taagcaagaa
     5401 aacaaaatta ctgctactat aggcattaaa acaaacaggc gaggtggcgc ccctgagcca
     5461 acaacaacga taaatataac cggaagtata taaatgaaaa tgattgatga aatcaaataa
     5521 attgatcttg tttttaattt atatgttagg gtaaacatca gaattaataa aggaatcagt
     5581 aaccacttgt ataggtttaa accagtggga ggattgttat agaagttata tatattatct
     5641 atggctgatt taacaagcca tttaatatcg cttgcatttt gtatataatt tgagatgtac
     5701 tcgttcgatt caagcgaaaa ataatgcttg atagctttgg ttaatagcag gtaaattaag
     5761 gtagataaag ctatgaggga tagagataaa catgaactat aaaaagattc acgaatattt
     5821 ttctcatttc gtattatcga atttatctgc aacccaatga cgaatgcaat aatatatgtt
     5881 acgaatgttt ggtagattgc cattgaaaga atggacagta ctataccaga aaatatcaca
     5941 atcctatttt tttgcgagtg aaaaataatt gctgatatcg ctgccagtag aaaagcaatt
     6001 cccacagtat cagcttggtt aagaaactct aattgatagg agatctgagg gaaggtaata
     6061 aatactaaca tacctatcaa taattcataa gattctagct ttagcgatct gcagattata
     6121 aatgctgaaa tgatgataaa agataaggct attaatggtg ttatataaag tgaaaaaggc
     6181 tcagggagaa agtaatgtcg caaaaaagtg tgaaaccacc gcccaagcgt gattgtttga
     6241 taaaaattat ttgtaaactc gccatcaata ttaagtgttc catcgtacaa ccacccccaa
     6301 taacaataaa ttgatattgc aaaacatgat atgaaggcaa ggagatttgc atttttaaat
     6361 aaattaattt taatcatggg ttacctcgat gagatttttt tatgatgtat ttgggtctat
     6421 tttttacttc aatatagatt ctacctatat attctccgag aacaccaatc ccgatcagtt
     6481 gcactccacc caagaaaagt attgatacaa gcagggaggg atacccgcgt actgggttac
     6541 caaagacaag ggtgtctata atcatccatg caccatataa aaatgaaatg cttgcaacaa
     6601 acaagcctat ataagtccat acgcgaagag gaaaggttga aaaacttgtg ataccttcca
     6661 gtgccagatt ccataatttc cagccattaa attttgagat gccagcaaca cgctctgcgc
     6721 gtacatattc aacgacatcc gtcggaccac ccacccagct cagtatgcct ttcatgaaaa
     6781 gattgcgctc aggcaacagt ttaatgttct ccacaacctc acgagacatg agtcgaaaat
     6841 ctccgacatt ttcctcgatc tttggggtgc ttattttgtt gtgtaattta tagaaccact
     6901 cagctgtctt acgtttcagt cgtccatcag ttgagcggtc tgaacgttta gcaagcacca
     6961 tgtcagcacc tgcctgccac ttttcaataa gatgagggat aacctcaatt gggtcttgca
     7021 ggtcgacatc aataggaatt acagcatcgc cgcttgcatg gtctaaccct gcaaataagg
     7081 ctggttcttt accaaagttg cgtgtaaatg acagcggaac aactagcggg tctgaaacag
     7141 ccagcgcgtt aataattgac tctgtggcat ctttacttcc gtcatttatg aatacaattt
     7201 ctacttcata tggcttcaac tcttggaatt cacgtaccgt tttatagaaa acaggtatcg
     7261 cttcttcttc attgaagaca ggaacgacaa gagatatttt catttcgcat ccctaaagac
     7321 aatgaacttt gaatagacga aaccgcacac cagactgatg gcggagaagg tgataagagt
     7381 gactattgga ggaagtgagc atttatcagc tgcccaacca acaatcacac tcaatattcc
     7441 cataaatccc acgtataaca tgtagcgcat cgctgtagtc aatgctttga atgtgaatct
     7501 tgcattcgcg aagaagctaa agctcacagc cactacgaaa cctgtgaagt ttgccagagc
     7561 ctgactggta tgtgcggcat agatacatac accaaaaacc acccagtgta taagtgtgtt
     7621 cagcacacct atagatgtgt actttacaaa taactttaac atttatttaa tcaatgagct
     7681 ctgaaaggca tgaagtctat catccaagtc tcaattgatc gatacttgct atgtctgatg
     7741 agacaaaact gagacacata aggcctcaca atggcttgca aggctttaca tgttttgatg
     7801 tggtgggacg tgtgagcgca gtgttgatgg gataatcctt tgaattacaa gcggattctt
     7861 ataattcgta atgcgaaggt cgtaggttcg actcctatta tcggcaccat ttaaatcaat
     7921 aagttacctc gcatttaagt aaactacgtt ctcctcttgt gccgtatttg tgccattgcg
     7981 acttataatc gcatcgattt tgctcgcgtg ctcggtaaga tgcccggctg aaaggtgggc
     8041 gtatctttga accatttcga gagtttccca tcctcccatc tctttaagcg caagaagaga
     8101 gacaccggac tgaaccagcc agcttgccca ggtatgcctc aggtcatgga agcggaagtt
     8161 gctaatgcct gcccgcttta acgctccctt ccatgccttg ttgctgtcgg ttctcatctt
     8221 ccttaccgct gctgtttttg ttccgtcgct tcggtaggca ggtttggtgt ggacaaacac
//
LOCUS       DAETQL010000145         8224 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.147, whole genome shotgun sequence.
ACCESSION   DAETQL010000145 DAETQL010000000
VERSION     DAETQL010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.147"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..305
                     /locus_tag="K0W65_003801"
     CDS             <1..305
                     /locus_tag="K0W65_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HBI3096892.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            277..2187
                     /locus_tag="K0W65_003802"
     CDS             277..2187
                     /locus_tag="K0W65_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBI3096893.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2188..2391
                     /locus_tag="K0W65_003803"
     CDS             2188..2391
                     /locus_tag="K0W65_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3096894.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2388..3981
                     /locus_tag="K0W65_003804"
                     /pseudo
     CDS             2388..3981
                     /locus_tag="K0W65_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3971..5476
                     /locus_tag="K0W65_003805"
     CDS             3971..5476
                     /locus_tag="K0W65_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBI3096895.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5513..5860
                     /locus_tag="K0W65_003806"
     CDS             5513..5860
                     /locus_tag="K0W65_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBI3096896.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5918..6946
                     /locus_tag="K0W65_003807"
     CDS             5918..6946
                     /locus_tag="K0W65_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBI3096897.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6998..7384
                     /locus_tag="K0W65_003808"
     CDS             6998..7384
                     /locus_tag="K0W65_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBI3096898.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7396..7773
                     /locus_tag="K0W65_003809"
     CDS             7396..7773
                     /locus_tag="K0W65_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3096899.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7760..>8224
                     /locus_tag="K0W65_003810"
     CDS             7760..>8224
                     /locus_tag="K0W65_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3096900.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1905 a         1932 c         2541 g         1846 t
ORIGIN      
        1 tactcattgc cagatgggaa ctgaccgcag aacaggctgt cgcacagcag ttaaaaaatc
       61 aggtgtcaaa aggaaatctt atcgataccg gattttgtat ttttgccctt agtaagctgg
      121 cgatggcttt atccagtacg cttgactcca ttcctttgtc catgcaacga cagttcccgg
      181 atttaacgcc gcgtcatatt gatcatctga aaatccttat tgctaaggga gcgaatcagt
      241 gcgcgcgtgc gggggataaa ttaccggatt tactcgatga atatatcaga acaacaactg
      301 agtaatatga tggtggcagt cactatcgca ctgcagccac tggtcagggt attgccggtg
      361 acggctgtag aatgggccga tcaaaattat tacctgccta aagaatcgtc atacggtgag
      421 ggggaatgga aaacgctgca gtttcaggtc gccatcatga actgcatggg caatgacgag
      481 attcgtacag ttaacctgat taaatctgcc cgtgttggtt atacaaaaat ggtgcttggt
      541 gtgatcgggt attttattga gcacaaatcc cgcaacactc ttctttttca gccgacggac
      601 tcagctgctg aagattttat gaagtctcac gtggaagcaa caattcgtga tgttccttgc
      661 ctgaaaaaac tttctccctg gctgggccgg aaacaccggg ataatacgct cacactgaag
      721 cgtttttcct ctggtgtggg gttctggtgt ttgggtggtg ctgctgccaa aaactaccgt
      781 gaaaaatctg tcgatgtggt ctgctatgac gaactctcct cgtttgaacc ggatgtggaa
      841 aaagaaggtt cgccaacact gcttggcgat aaacgtattg aaggttcggt atggccaaaa
      901 tccatacgcg gttcaacgcc aaaaattaaa ggctcctgtc agattgaaaa agctgcgaat
      961 gaatctgcgc atttcatgcg gttttatgtc ccttgccctc attgcgggga ggagcagtat
     1021 ctgaagtttg gcgatgatgc gacgtcgttt ggcctgaaat gggagaaggg taaaccggaa
     1081 acggtgtatt acctgtgtga acataatggc tgcgtgatcc gtcagtctga acttgatcag
     1141 accggggggc gctggatttg tgacaatacc gggatgtgga cgtgtgacgg tctgacgttt
     1201 ttcagtgctt cgggtaatga aattccgccg ccgcgctcca tcactttcca tatatggacg
     1261 gcgtacagtc cgttcaccac cagggtacag atagtctgtg actggctgga tgcactgaaa
     1321 gatcccaacg gcgtgaaaac ttttgtgaac accacgctgg gcgagacctg ggaagaggcc
     1381 gtgggcgaaa aactcgatca ccaggtactg atggataagg ttgtgcctta cacggcaacg
     1441 gtgcctgtcc gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag
     1501 atgtatgtct gggggtgggc tccgggagag gaagcctttc tggtggataa aatcatcatc
     1561 atgggacgtc ctgatgagga agagacgctg ttacgtgtgg atgcggcgat caataaaaaa
     1621 taccgccatg cagacggtac cgaaatgacc atttcccgtg tctgctggga tatcgggggg
     1681 atcgatggcg aaatcgtgta tcagaggtca aaaaaacacg gtattttccg ggtactgccg
     1741 gtaaaaggtg cctctgtcta tggcaagccg gtgatcacca tgccgaaaac ccgcaatcag
     1801 cggggtgtgt atctgtgtga ggtggggacg gataccgcaa aagaaattct ctatgcccga
     1861 atgaaggccg atcccacccc tgtgaatgag gccacatcgt atgccatccg ctttcctgat
     1921 aatccggaga ttttttcgca gacagaggca caacaactgg tggcggagga actggtggaa
     1981 aagtgggaaa aaggaaagat gcgtctgttg tgggataaca aaaagcggcg taacgaagcg
     2041 ctggactgcc tggtgtatgc ctatgcggca ttacgtgtgt ccgtgcaacg ctggcaactt
     2101 gatctggctg tgcagttgca tcccgcaagg atgcaactgc acaaccgacc cttgaagaac
     2161 tggcagcgaa gctgtccgga ggagtgaatg gttacagtcg ctgaactgca ggcgctgcgt
     2221 caggcgcgcc ttgatttatt aaccggtaaa cgggttgtgt cggtacagaa agacggtcgc
     2281 aggatcgaat atacggcagc gtccctgaat gagcttaacc gtgcgatcaa cgatgcggag
     2341 tcggttctgg gtacgacccg acgccgccga ccgctgggag tgaggttatg aaacgaacgc
     2401 ctgtcctgat tgatgtgaac ggcgttccgc ttcgtgagag tctcagctac aacggaggcg
     2461 gcgcaggatt tggcgggcaa atggcagagt ggttaccacc ggcgcagagt gccgatgcgg
     2521 ccctgttgcc tgcattgcgt ctggggaatg cccgggcaga tgatctggtg cgcaataacg
     2581 gtatcgcggc taatgcggtg gccctgcata aggatcatat cgtcgggcac atgtttctta
     2641 tcagctaccg tctgaactgg cgttggctgg ggatgcggga gaccacggca aaaagttttg
     2701 tcggggaggt ggaagcagcc tggtcggaat acgcagaagg aatgtttggc gagatcgacg
     2761 tggaagggaa acgcacgttt acggaattta tccgtgaagg ggtgggcgtg catgccttta
     2821 acggcgaaat ctttgtgcag ccggtctggg atacggaaac cacgcagtta ttccgtacgc
     2881 gttttaaagc cgtgagtccg aaacgggtgg acacgccagg acacggtatg gggaaccact
     2941 ttctgcgggc cggggttgag gtcgatcggt atgggcgggc gattgcgtac catatctgtg
     3001 aggatgattt tccgttctct ggtgccggac gctgggagcg gatcccgcgt gagcttccca
     3061 ccgggcgtcc ggccatgctg catatttttg agccggtgga ggacgggcag acccgtgggg
     3121 ctaatcagtt ttacagcgta atggagcgga tgaagatgct ggactccctg caggccacgc
     3181 agcttcagtc ggccattgtg aaggcgatgt atgcagccac aatcgaaagt gaacttgata
     3241 ccgaaaaggc atttgaatat atcgcggggg caccgcaagg tcagcaggat aatccgctta
     3301 ttaatattct ggagaagttc acccgctggt atgacacgaa taacgtgacg ctgggcggtg
     3361 ttaaaattcc gcaccttttt tcctggcgat gcgctgaacc tgcagacagc gcaggattca
     3421 gacaatggat tttcggcgct tgaacaggcg ctgctgcggt atattgcagc cggtcttggc
     3481 gtttcctacg aacagttgtc ccgtgattac tcgaaggtca gttactcaag tgcccgcgcc
     3541 tccgccaatg agtcgtggcg ctattttatg gggcggcgaa aatttattgc ggcccggctg
     3601 gccacgcaga tgttttcctg ctggctggaa gaggcgcttc ttcgggggat tattcgtccg
     3661 ccacgggcgc gttttgattt ttaccaggcg cgttcagcct ggtcacgggc agagtggatt
     3721 agctccggaa gaatggccat tgacgggctc aaggaggtcc aggaatcggt gatgcgcatt
     3781 gaggccggac tgagcacgta tgagaaagaa ctggcactga tgggcgaaga ttatcaggac
     3841 attttccgcc agcaggtcag ggaatccgca gagcgcgaaa aagccggact ctcacgtccg
     3901 gtgtggatag cccaggcgta tcagcagcag atagcggaga gtcgcaggcc ggaagaggag
     3961 acaacatcac gtgagacgta atctttcaca tattatcgct gcggcattca atgagccgct
     4021 gcttctggag cccgcctatg cgcgggtttt cttttgcgcg ctcgggcgtg agatgggcgc
     4081 agcaagcctt tcggtaccgc aacagcaggt acagcttgac gctcccggga tgctggctga
     4141 aacggacgag tacatggccg gaggcaaacg acctgcccgt gtttaccggg tggtgaacgg
     4201 tattgctgta ctgccggtga ccggcacgct ggtacaccgg ctgggtggca tgcggccatt
     4261 ttccggaatg actggctatg acggcattgt cgcctgtctt cagcaggcaa tggcggacac
     4321 tcaggtacgg ggcgtactgc tggacattga cagttctggc gggcaggcca ccggcgcgtt
     4381 tgactgcgct gacatgattt accgtctccg gcagctgaag cctgtctggg cactgtgcaa
     4441 tgacacggcc tgttcagccg ccatgttact ggcatcggcc tgctcccgac ggctggttac
     4501 ccagacatcc cgtatcggct ccattggggt gatgatgggc catgtcagct atgccggtca
     4561 tctggcacag gccggtgtgg atatcacgct gatttacgcc ggatcgcaca aggtggatgg
     4621 caatcagttt gaatccctgc cagcaaaggt tcgccaggac atgcagcagc gcattgatgc
     4681 ggcgcgccgg atgtttgctg aaaaagtggc gatgtatacc ggtttatctg tggatgcggt
     4741 tatgggaaca gaggccgccg tttttgaagg tcagtccggt attgaggccg aactggcgga
     4801 tgaattaatc aatgcgtcgg acgccatcag cgtgatggct gcgacgctga acacacacga
     4861 tacaggaggc actatgccgc aattaactgc aacggaggct gccgcgcagg agaaccagcg
     4921 agtgatgggg atcctgacgt gtcaggaagc gaaaggacgt gaacaactgg ccacgatgct
     4981 ggcagggcaa cagggtatga gcgttgagca ggcccgggcg attctggccg tggcggcacc
     5041 gcaacaatct ggtgtatctg cgcagagtga agccgatcgc attatggcat gtgaagaggc
     5101 gaaaggacgt gaacaactgg cggcaacgct ggcggcgatg ccggagatga cggtggaaaa
     5161 agcccgtccg attctggctg cttcaccgca ggcgaatgcc ggaccctcac ttcgtgatca
     5221 gattatggcc ctggatgagg caaaaggggc agaggcgcag gctgaaaaac tggcggcctg
     5281 tccggggatg accgtggaga atgcccgggc tgtactggcg gcagcctcag gcagggcaga
     5341 accggtttct gcatccacaa ccgccatgtt tgaacatttc atggcgaacc attcaccggc
     5401 agcggtacag ggtggcgtgc cacagacgtc agcagacggt gatgcggacg tgaaaatgct
     5461 catggccatg ccatgaagtc agtgctgacc atcaatatga ggtttttaca atatggtaac
     5521 gaaaaccatc actgaacagc gtgcggaagt acgtattttt gccggtaatg atccggccca
     5581 taccgccaca ggctgcagcg ggatttcctc ggcaacaccg gctctgacgc ccctgatgct
     5641 ggatgaggat accgggaaac tggtggtctg ggacggacag aaggcaggcc atgtggttgg
     5701 catactggta ctgccgcttg aaggtacaga gacggtgctg acgtattaca agtcggggac
     5761 ctttgcgacg gaggccatcc actggcctga gagagtggat acacacaaaa aggcgaatgc
     5821 ctttgccggc agtgccctga gtcacgcagc gctgccgtaa cattttatca ggtcactgcg
     5881 gtagcctgac tgatttctga atgaaaggaa ctgatttatg ggattgttta cgacccgcca
     5941 gttactcggt tataccgaac aaaaagttaa atttcgtgcg ctgtttctgg agctgttttt
     6001 ccgccgtacg gtgaatttcc ataccgaaga ggtaatgctg gacaaaatta ccggaaaaac
     6061 gccggtggcg gcctatgtct ccccggttgt tgaaggaaag gtgttgcgtc accgtggcgg
     6121 tgaaactcgt gtactgcgcc cgggctacgt taagccgaaa cacgaattta attaccagca
     6181 ggcggtggag cgtcttcccg gtgaagatcc ggcccagctg aacggcccgg cttaccgtcg
     6241 cctgcgtatc atcaccgata acctcaaaca ggaagagcac gccattgtcc aggtggaaga
     6301 gatgcaggcg gtgaatgccg tgctgtatgg caaatacacc atggaaggag agcagtttga
     6361 aactgttgag gtcgattttg gccggtcggt gggaaataac attacgcagg caggagggac
     6421 ggagtggagc gctcaggacc gcgatacctt tgatccgacg catgatctgg atgcgtactg
     6481 tgattttgct tccggcacca tcaatattgc cattatggat ggtaaggtct ggcgtctgct
     6541 gaatggcttt aagctgttcc gcgaaaaact ggatacccgt cgtggctcaa attcacagct
     6601 ggaaacggca gtgaaagacc tgggggcggt ggtgtccttc aaagggtatt atggtgatct
     6661 ggcaatcgtg gtggcaaaaa cgtcctatgt ggcagaggac ggtaccgaaa aacgttatct
     6721 gccggacggc acactggtac tgggaaatac ggccgcagag ggtattcgtt gctacggtgc
     6781 cattcaggat gcgcaggcat tgtctgaagg cgtggtggcg tcttcccgtt atccgaaaca
     6841 ctggctgacc gtgggtgatc cggcgcgtga attcaccatg acgcagtccg cgccgctgat
     6901 ggtgctgccg gatccggatg agtttgtggt ggtgcaggtg aaataatccg tgagcgggag
     6961 ctaaatgctc ccgtttttca ttcaatcagg agcgggtatg gctacgaaag aagaaaacct
     7021 gaaacgtctt tgtgagctgg ctgagaggct ggggcgtgaa ccggatgtct ccggtagtgc
     7081 ggcagatatt gcgcagcgag tggcggaact ggaggaagag cttggagatg cgggggaggt
     7141 cgcagagccg gatacgtact caccatcgaa agatgatccg accgcgcata aaggcgagtc
     7201 agttcctgaa cagccaggca ttgttaccgg agacgaaacg gggcaggtta cggtggtggc
     7261 actggcgaca ctgcatacaa aatgtaatgg ttcagtgata tttgtttcac cgggaacgtc
     7321 gtttcgtgtt tctgctgagg tggctgccag catggcggca ggtggtctgg caaaaaggca
     7381 gtaacaggag atgtcgtgga cggtttcgat aatctgtttg atgctgcgct tgccggtgtc
     7441 aatgaagtca ttctccgtga tatggggatc agcgccgtca ttacgtccgg agaacttgag
     7501 gggacacacc tgacaggggt ttttgatgat cccgaaagta tttcttttgt tgccgggggg
     7561 atccggcttg aggattcctc accgtgcctg tttgtgaaaa cggcagatat atcgcagtta
     7621 cgccgtcagg atacgttaac tatcggggat gattctttct ttgtggatcg tattactccg
     7681 gatgacgggg gatgctgtta tatccggtta aggcggggca gtcctgcccc acagaataat
     7741 gccagaatga ggcgatatga tgaagggact tgagaatgcc atccgcaatc tgaacagcct
     7801 cgatacccgc atggtgccac agaccagcgc atgggcggta aaccgtgtgg cggcaaagat
     7861 tgtttctgtc gccacacggc aggttgcgca gaataccgtt gccggggata accaggtgaa
     7921 aggtattccc ctgaaaacgg tcagggagcg tgtcaggctg cttaaagcca gcccctcagg
     7981 aaaaatgtat gcccgcatgc gtgttaaccg gggtaacctg cccgccatta agctgggtac
     8041 cgcacaggtc agactggccc gctcccggca tggcagcaac tcacgtcatc gtggcagcgt
     8101 actgaaagtg gggaaatacc ttttccggga tgcgtttatt cagcaactgg cgaatggtcg
     8161 ctggcatgtg atgcggcgta ttgacggcaa aaatcgttac cccattgatg tggtgaaaat
     8221 cccg
//
LOCUS       DAETQL010000146         8070 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.148, whole genome shotgun sequence.
ACCESSION   DAETQL010000146 DAETQL010000000
VERSION     DAETQL010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.148"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(106..297)
                     /locus_tag="K0W65_003811"
                     /pseudo
     CDS             complement(106..297)
                     /locus_tag="K0W65_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            295..619
                     /locus_tag="K0W65_003812"
                     /pseudo
     CDS             295..619
                     /locus_tag="K0W65_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(649..3561)
                     /locus_tag="K0W65_003813"
     CDS             complement(649..3561)
                     /locus_tag="K0W65_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096901.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKVFYERSEKSVGYWVGGIRRAAQKA"
     gene            3564..3680
                     /locus_tag="K0W65_003814"
     CDS             3564..3680
                     /locus_tag="K0W65_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBI3096902.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            4044..4886
                     /locus_tag="K0W65_003815"
     CDS             4044..4886
                     /locus_tag="K0W65_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBI3096903.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4889..5977
                     /gene="capU"
                     /locus_tag="K0W65_003816"
     CDS             4889..5977
                     /gene="capU"
                     /locus_tag="K0W65_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HBI3096904.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5982..6932
                     /gene="virK"
                     /locus_tag="K0W65_003817"
     CDS             5982..6932
                     /gene="virK"
                     /locus_tag="K0W65_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HBI3096905.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6997..7941
                     /gene="lpxM"
                     /locus_tag="K0W65_003818"
                     /gene_synonym="msbB"
     CDS             6997..7941
                     /gene="lpxM"
                     /locus_tag="K0W65_003818"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBI3096906.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2171 a         1809 c         1925 g         2165 t
ORIGIN      
        1 ccggggcggt tcaaagggcc ttcccgagag tggtaaagga gaggccctgg aaatacccca
       61 cagccccgaa aaagagccag tcagttgctt aactgactgg cattaaggcc gccggggacg
      121 tttttttacc accgggagtt tgggcataac ggtgctttca gtaagtacga caggtactgt
      181 ggaggcactt ccgtaaagaa gggcatcaag ctcctgctcc gacatacctg caggcaaagg
      241 ccatgaaagc cctgatttcc ggaagcgagc gaacagtcca caaactgttg aacgtggtgt
      301 cagcggcgga cagggataag ccctcgtcag cagttccgtc agcactgcga cagcgttctt
      361 tacccggtca aaacagcgtt gcggtgcacc acgcctgacg gatgaactgc gtgctcaggg
      421 ttaccacttt aacgtaaaaa ccgtggcggc aagcctgcgc cgtcaggggc tgagggcaaa
      481 ggcctcccgg aagttcagcc cggtcagtta ccgcgcacat ggcctgcccg tgtcagaaaa
      541 tctgctggag caggattttt acgccagtgg tccgaaccag aagtgggcgg gagacatcac
      601 gtacttacgt acagatgaag ttcggttaca tcctgtcagc acggaacctc acgctttttg
      661 agcggcacga cggatccccc ccacccagta tccgacagac ttttcactgc gttcgtaaaa
      721 aaccttcttc cctcccagca tatccactaa ttccggtgac atatcctgca gataagtctg
      781 caaatcatgc cccagatctt ccggtgactg caactcataa accgcgcgcc acacatcacc
      841 cttccttatt ttaagccccg ggttaagaat atactgtccg tggttcagcc tcagaaaaag
      901 cttttttcca taaggatttt tgctgttaac ttcatgttga gagagctggc tgctgatata
      961 actccttcgt ttgcggcgtt ccggcagcaa cgcttcagga agatgaacca cacactctgc
     1021 cagtcgtgcc gcactgaagg cttccgaaaa aaacagagca cttcccagat gcgtgtggaa
     1081 cagcgcctgc atcaggatca ccagaaacat tgccatctgc cggttgtcca gtttgatgag
     1141 gcgtccttcc acctgcagtg acagactttc aggtttcagc agggtataca actgcgcact
     1201 tttctgttgc gcataacggg gcgtactgac cgccgctgac agaacttgat gataagccgc
     1261 cagcccgtca ttaccggtca gcaagggatc tgcaccacgc tccagcagta actgaaccag
     1321 tgcaatatta cctgcccggg ctgctgacat cagcggagta agattaaacg gcgaacggtg
     1381 atcaacacca taacgttcaa tatcccgcaa cacggcaacc gggttattca ttgagtatgg
     1441 ccggaaatac ttcagctcca gtaactgcag agtcttcgtt ctgggctgcc ttgtgggctt
     1501 aaaatcttcc cggtacagtg cattatacag cgggaacagg gaataaatca gactgtactc
     1561 cagcgcctgc agttgtatgg ttttatcggc tgtacctgcg aggatatgct gtttccactg
     1621 tcgtgcatcc tctgcggtga taatctgcca tggcatctca gatgtctgca ggatccggct
     1681 acggatggtg tccgcctgct cctgcttccc ctgtttttca agcagatgtg cggttttctg
     1741 ccactcatca cggctggaaa tctcttcctg cagattcaga ggctcttcct gatgcgtgag
     1801 cgcaagcaga ctccacagag gatgctccac ctgctgttca acaagataca cattgtgttt
     1861 agcacgggtg agcgcaacat acagcgcgtt ggtaaaaaat ttatagattt ccgctgaacg
     1921 atcctgcctg tcccgcggac gggaataacg ggcctcgccc tcaagatcag ccggtgtaag
     1981 accctcgcaa atatcatcaa atgcctgccg ggcagcgctt actatattgt aaaggatcac
     2041 cgtttcgtat tccagcccct taacctcctg aaccgaaaag accagcggcg tactgaacca
     2101 gcatcgcgcc tgtgctttct gttccggatg catcacaatg accgctacac gattcgaaag
     2161 tgaggttttg gcattcagtt cctgctttgt ctcttcccta tcgtccagca accggatggt
     2221 cccttctgcc tgaccgcagc ttctgacaaa atggtgactt tcccgatcaa tagcactaaa
     2281 gcggacctgt ttgagtctca gcaaccggtt agccagtgca gtgacgtggt gcccgttacg
     2341 gtaattcgcc tgcaggatat taacggttgt gtcgtttccc tgttgctgcc ggaaaaagag
     2401 acttttgagc gaactccatg agaaaaagct cggatgaact atctgattgg catcaccaca
     2461 gagtaaaaac tgaccgggat gacgtagcgt tttcagtacc agttgcagtt gaacaggcgt
     2521 catatcctga acttcatcaa caaaaatgac atcataacgg ggagttgcca acgaaagata
     2581 ctgatgactg agcagattac tgtcatactg atgcgactgt ttcagccatg cgatataacg
     2641 ttcaaacagg acatagaccg tcgggcggtc ctccatgcca tagagcgact gccgtatccc
     2701 cagtgacaga tacgcctccc gggacagcgg cccattcccg gaggcaaccg caccgatcac
     2761 gcccctgaac tcctcataca gggtatgggc agcccccagt gcggcgatag ccctgttgcg
     2821 gggcagccag tcgctgaacg cactgaaagt cacctccctg ccttccggaa tgcgcagagt
     2881 ttccagaaat tccgtcagcg aaagaaagtc aatattctgt acctcactcc cttcccctga
     2941 agcgtcatag agagtacgcg ctttttccac cagaaatgag gaaaggctga gatacagtat
     3001 atcaccagca gcctgcttca ttttttccag taccagcgcc gtcttaccac ttcccgccga
     3061 gccaacaatc accagcggta gcgggtgttc gtacagtgcc tgctgatcat catcaaagga
     3121 cagcatttta tccaggcggt ggaaacgccc atgagacgga ttgatatacg tcagcgcttc
     3181 agtcgcgata tcaacaggat ccggcactgg ttgttgttgt aagcgccctt catcaatcac
     3241 cacgtttctg cgaagaaaac gtgaagtgtt ataggcatga ttgcgaatat attccagaac
     3301 aaggcagata gtttcaccgt ggtagcgata cagcgaaaac agtaatcggt cccggatact
     3361 gagccgggcg cggtaaagat tggtgtcaat tttacgaaca tcagcctgaa caaagttatc
     3421 ggtggcaata gcctgtgcaa attttttata acccgggatc gtgtcaggat tcagagtatg
     3481 ataaagacag attttcatgg ctgccggttt ccgtcgtgca aaaaacatgc tgattttacg
     3541 aaggaatgcc ttactgaaca accttgcccg gaataccccc cgaaatcaga tggaacaatc
     3601 agaaaaatgc tctgcagtta tggaataaag aaggtggggt ggtacgccgg gagggcgatc
     3661 cgctggaaat ggcgcagtag ctgcaggcct gtcatgatgc aggaatagaa gttcgtgtac
     3721 aggttaatga aagcgtcacc ccgtaaataa actctgcgct gaaattcctt tttttcattg
     3781 gagtacctgt gttgttctga ggtgaacata tcacctctgt tcaggtagcc aaattcagtg
     3841 tgccacctca aggttcttag tgagtcgttt ttaaaagata tgataatata tcattgttta
     3901 tgaattgttg atgtttaatt tagtaaatta tatcaaatat gccatgggtt tgctatgtta
     3961 ttcatccagt atctggaaat aaaatgcacc agctttattg ggttttcctt ttgtctctgc
     4021 ttgctaggtg ccagaaactt taaatgttaa acgaaggggg cattttgttc aaagccaatc
     4081 atgttccggt attaatgtat catcatgtca gtcattgccc cggactggtt acactttctc
     4141 ccgtgacatt ccggaagcaa ataaaatggt tggctgaaaa caactggaaa acactgtcat
     4201 cagatgaact ggagtttttt tatcggggcg gaaaactccc ccggaaaagc gttatgttga
     4261 catttgatga tggatacctt gacaactggt ttcaggtata tcctttatta aaagagttta
     4321 atctcaaagc acatattttt ttaattaccg gttttattgg gaatggtccg gttcgtcact
     4381 ctcctggaaa ggaatattct catcgcgatt gcgaacatca aatagcgaca gggaatgctg
     4441 ataatgttat gcttcgctgg tctgaagtaa acgaaatgtt gcaatctggt ctggtcgaat
     4501 ttcatgttca tacgcatact catacacgct gggataaaaa gttcagttca cgagaagaac
     4561 agtgtaaaca tcttcggcag gacttgttga gcgggaggga atatttgaaa gaaatgacag
     4621 gaaaatgcag taaacatttg tgttggccgg aaggttatta taacaaggat tatattcagg
     4681 tcgctgaaga acttggattc tattatttat atacaacaga aagaagaatg aatgctcctg
     4741 ctaaaggaac tacacggata ggaagaataa gcaccaaaga acgggaaagt tgcgcatggc
     4801 tgaaaagacg acttttttat tatacaaccc cgtttttttc atcgttgctg gcctttcata
     4861 aggggccaag attaccggat gattaataat gaatatacta tttacggaat catcaccgaa
     4921 tataggtggt caggaattac aggctgttgc tcaaatgaag gccctgaaga aaatggggca
     4981 ttcagttctg cttgtctgca gggaaaacag caaaattgct tttgaagcca gtaaattggg
     5041 gattgatatc acattcgcgt tatttcgaaa cagtcttcac atccctactg catggagatt
     5101 actcggaata gttcatggtt ttcagcccaa tgcaatcgtt tgtcacagtg ggcatgatag
     5161 caatattgtt ggtttagtac ggttattcac taggaaacat ccattcagaa ttatcaggca
     5221 aaagacatat ttgacacgaa aaacaaaagt tttttcaata aatcattttt gcgatgaggt
     5281 gattgttccc ggaacaagta tgaagacaca tctggagcag gaaggatgtc gaacccgggt
     5341 tactgttgtg cctccaggct ttgatttcca gaaattatac gttgattccc gaaacagttt
     5401 gcctccaaat gttctttctt ggctggcgtc ccgaagggga tgccccgtta ttgctcaggt
     5461 aggaatgttg cgcccggaaa aagggcacga atttatgttg aatttactgt tccatttaaa
     5521 aatgaatgga cgacagttct gttggttgat tgtggggtct ggttcgcctg aactgcggga
     5581 gcatttacag tatcagattg acagtatggg catgcatgat gatgttttta ttgctgacaa
     5641 tgtttttcct gccgcccccg tatatcgggt tgccagtctg gtggttctgc cttcagaaaa
     5701 cgaatcgttt ggtatggtac tggcagaagc atcggcattt tctgtgcctg tactggccag
     5761 tcagattggt ggaatccctg atgttattca gaataaccag accgggacat tgttaccagc
     5821 aggtaataag cacgcatgga tgtgcgccct gaatgatttt tttaatgacc ctgggcgttt
     5881 ttatcagatg gctcgccagg caaaacagga tatagaagag cggtttgata ttaataaaac
     5941 tgcgttaaaa atactcacat tagcgaagca caagtaacaa tatgttttct gtaagtaact
     6001 tatcatttat cggtttcctt aaaaggattg ttttttcctc agattcactc ccggggaagt
     6061 gggaacacag aaaattccgg ttcatgtaca ttttgcgatg tgctataaat ccggttgcca
     6121 gtattcgata ttattacgaa ctgcgttcct tgcagtgcat tgaggatatt ctggcaatac
     6181 aacccacgtt gccagcaaga attcatcgac cttatttgca taaagggggg cgagcctggt
     6241 cccgggggca atatattctt gagcattatc gctttgtgca aaatttgcca gaaaaatatt
     6301 ctgaattttt gtttccacaa aaatcggtat cattagtcca attcatcggg aaagatggag
     6361 aggattttga tatccagtgt tctcccagtg gttttgaccg ggaaggagag ttgatgttgt
     6421 cattgttttt caataaaata gtgattgccc ggttaacatt ttcagtcatt ctgactcaaa
     6481 atggacacac cgcttttatt ggcggattac agggagcccc caaaaatacc ggacctgata
     6541 ttattcggtg cgcaacccgg gcctgttatg ggcttttccc aaaacggatt atttttgaag
     6601 cattttgtgc gttgatgaaa gcttgtaatg tctctgagtg tctggcagtc agtgagcaca
     6661 gtcatgtttt ccggcaattg agatactggt atcagaaacg caaaaccttt gttgctgtct
     6721 acagtgattt ctgggagtct gtagcaggga aaacctgtgg tgactggtac aaattaccaa
     6781 cacaggtggt tcgaaagccg ctaagcaata tagctagtaa aaaacgctct gagtatcgaa
     6841 aacgatacgc tttgctggat tatatccatg aaactgctat ccgctctttg gatgcatatc
     6901 ctgtaaactc agaacatcag gacttaaatt aaggtttgat gtattctctg aataaaatat
     6961 taatgatgat tttggtaggg gcattcgcac taaataatga aaaaatacaa atccgagttt
     7021 attcctgaat ttaagaaaaa ttatctttcc cctgtttact ggtttacatg gttcgttttg
     7081 ggaatgattg caggtatttc aatgtttccc ccttcattca gagatcctgt cttggccaaa
     7141 atagggcgtt gggtgggcag attgagcaga aaagctcgtc gcagggcgac gattaattta
     7201 tcgctttgtt tcccggaaaa gagtgataca gaacgggaaa taattgtcga caatatgttt
     7261 gccacagcat tgcaatccat agtgatgatg gcagaactgg caattcgtgg tccggaaaag
     7321 ttccagaaac gagtgttctg gaaaggcctc gaaattcttg aggaaattcg gcacaataac
     7381 agaaatgtga tttttctggt tccccatggc tggagcgtgg atattcctgc aatgttgctg
     7441 gcagctcagg gggaaaaaat ggccgccatg tttcatcagc aacgaaatcc agtgattgat
     7501 tatgtctgga attctgtacg gcgtaaattc ggggggcgct tacattcccg ggaggatggg
     7561 ataaaaccat ttattcagtc agtacgccag ggatactggg ggtattacct tccagatcag
     7621 gatcatggtc ctgaatacag tgaatttgct gatttttttg cgacctataa agcgacatta
     7681 ccaattattg gacgtctgat gaacatcagt caggctatga ttataccgct tttcccggtt
     7741 tatgatgaaa aaaaacattt cctgactatt gaagttcggc caccaatgga tgcatgcatt
     7801 gccagcgcgg acaataaaat gattgcccga caaatgaaca aaacagtgga aattttggtg
     7861 gggtcacatc cggaacagta tatctgggtt ttaaaattgt taaaaacgcg caaatcaaac
     7921 gaagcggacc cgtacccttg aaattataaa gtacaggtat ttccactagt tgtttcttac
     7981 aggttaccaa tcgaaacaca tcccccttcc gtgtgttgcc cgtttggcac ggaggactgt
     8041 gtagatgcag ttcgttaaat ttaatttatg
//
LOCUS       DAETQL010000147         7982 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.149, whole genome shotgun sequence.
ACCESSION   DAETQL010000147 DAETQL010000000
VERSION     DAETQL010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7982
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.149"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..159
                     /locus_tag="K0W65_003819"
     CDS             <1..159
                     /locus_tag="K0W65_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096907.1"
                     /translation="ISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGE
                     DVEPGDDF"
     gene            complement(168..566)
                     /gene="vapC"
                     /locus_tag="K0W65_003820"
     CDS             complement(168..566)
                     /gene="vapC"
                     /locus_tag="K0W65_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HBI3096908.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(566..793)
                     /gene="vapB"
                     /locus_tag="K0W65_003821"
     CDS             complement(566..793)
                     /gene="vapB"
                     /locus_tag="K0W65_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HBI3096909.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            875..1246
                     /locus_tag="K0W65_003822"
     CDS             875..1246
                     /locus_tag="K0W65_003822"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HBI3096910.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1267..6128
                     /gene="traI"
                     /locus_tag="K0W65_003823"
                     /pseudo
     CDS             1267..6128
                     /gene="traI"
                     /locus_tag="K0W65_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6148..6894
                     /gene="traX"
                     /locus_tag="K0W65_003824"
     CDS             6148..6894
                     /gene="traX"
                     /locus_tag="K0W65_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HBI3096911.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6949..7509
                     /locus_tag="K0W65_003825"
     CDS             6949..7509
                     /locus_tag="K0W65_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBI3096912.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7640..7852
                     /locus_tag="K0W65_003826"
     CDS             7640..7852
                     /locus_tag="K0W65_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBI3096913.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1815 a         2029 c         2579 g         1559 t
ORIGIN      
        1 atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca gcaggaaaat
       61 catccggaca cctggcagca gatgcagcgt cgtgaagagg tgaacatcaa tgtgcaccgg
      121 gaacgcgggg aggatgttga gccgggagat gatttctgat gaacaggtca gctccagtct
      181 tcagttctca ggccgcccac acgttcaaat tcccgggtgt tattagtcac aataatcagc
      241 ccccgactgc gggcatgacc tgcgatcatt tgatcaaatg gcccgacagg gcgtccctga
      301 cgggcaagtt ctgctcttat ctggccggtg tgtgtggctg cagcagcgtc gtaatccaga
      361 acatcaatgc gggaaacaaa tccctcgatc acagcgagat tacgttcagg catctggctt
      421 ttttctgcac catatatcag ctccatcagg gtgaccgaac tgatacacat tttcccctgg
      481 ttcaggttaa aacgctccct gacgctggcg ggtttgttct ttatcgtaaa aatgcagatg
      541 ttggtatcga gcataaactt cagcatcaga atgactccct ttcttgcatg ccgggctgtt
      601 ccctgttatc cataaaatcg gtgctgacgc tgtgtccgtc gaaccattcg tcccatgtct
      661 ctccggctgg cgtaatgatt ctggttcgtc cgacagcaat cacttcaacg cgttttacgt
      721 tttccggcaa tgcaaccgct tttggcagtc tgaccgcctg gctgcggttg ctgagaaata
      781 cggtggtttc cattatttgc ctccttatgt ggatatgtct tgtgtatatc tattgtggga
      841 ttgataccgg gatatgtcaa agggatatac gtttatgatg agtatcgcgc aggtcagatc
      901 ggccggaagt gccggtaatt attataccga caaggataat tactatgtgc tgggcagcat
      961 gggagaacgc tgggccggcc agggggctga acaactgggg ctgcagggca gtgtcgataa
     1021 ggatgtcttt acccgccttc tggagggcag gctgccggac ggggcggatt taagccgcat
     1081 gcaggatggc agtaacaggc atcgtcccgg ctacgacctg accttctccg ctcccaaaag
     1141 tatctccatg atggccatgc tcggggggga caaacgtctt attgaagcac ataaccaggc
     1201 cgtggatttt gctgtccgtc aggtggaggc cagtgcctcc acctgacgga cggacagtca
     1261 gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat
     1321 caggaaccac agttacacac gcatgcggtg gtgactaatg ttacgcagta taacggcgaa
     1381 tggaagacac tgagcagtga caaagtgggg aaaacggggt tcattgagaa tgtgtacgct
     1441 aatcagattg cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg
     1501 ggctatgaaa ctgaagtggt cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg
     1561 gaggcctttt ccggacgctc acagactatc cgggaggccg tcggggaaga cgcctcgctg
     1621 aaatcccggg atgtggcggc gctggatacg cgtaaatcca aacagcacgt cgatcctgaa
     1681 gtcagaatgg ccgagtggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat
     1741 cgtgacgcag cggaacaacg ggcatatacc cgcacgcaga cacccggacc cgcttcacag
     1801 gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgcaaa
     1861 gtgcagttta tgtatacgga cctgctggcc aggacggtcg gcatactgcc gccggaaaat
     1921 ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc
     1981 cccctcgatc gtgagaaggg gctgttcacg ttcgggattc atatgctcga tgagctgtca
     2041 gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa
     2101 agtgtccccc ggacggccgg ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg
     2161 tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa
     2221 ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc
     2281 tcgcagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt
     2341 cagttgctgg aaggaatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa
     2401 aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag
     2461 gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcactgat ggccatgaag
     2521 gatgccgggg tgaacacata tcgctggcag gggggagaac agcgaccggc caccatcatc
     2581 agtgaaccgg accgtaatgt cggctggccg gagattttgc ggccagcgtg aaagccggag
     2641 aagagagcgt ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc
     2701 gcagtgagct gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt
     2761 caccggtctg gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg
     2821 tgatggagca gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga
     2881 cggcgcagag tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta
     2941 tttcttccct ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg
     3001 gtgagcgtct gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc
     3061 tgcaggtggc atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac
     3121 cggccaccct gcctgtgagc gattcaccgt tcactgcact gaagctggag aacggctggg
     3181 tggaaacgcc cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga
     3241 tggcaatgga caatgccacc ctgaatggtc tggcccgcag tggtcgtgat gtccggctgt
     3301 attcctcact ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg
     3361 tggtttctga gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc
     3421 tgcagaaagc cgggcttcac acgccggcac agcaggccat tcatctggcc cttcctgtgc
     3481 tggaaagtaa aaacctggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg
     3541 ctgcagaagg aaccggtttt gctgacctgg gaggggaaat caatgcgcag ataaaacggg
     3601 gtgatttact gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg
     3661 cgtcgtatga ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca
     3721 cgccgctgat ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg
     3781 ccgccacccg tatgatactg gaaacgtctg accgtttcac ggtggtgcag ggctatgccg
     3841 gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga
     3901 gtgaacgtcc ccgtgtcgtg gggctggggc ccacacaccg tgcggtcggg gagatgcgca
     3961 gcgccggcgt ggatgcacag acactggcgt cctttctgca tgacacgcag ctgcagcagc
     4021 gcagcggaga aactccggat ttcagcaaca cgctgttcct tctcgatgag agctctatgg
     4081 tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgggctg
     4141 tggccagcgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc
     4201 agcagacgcg cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg
     4261 aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc
     4321 ttgagcgtgt gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact
     4381 ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga
     4441 tgctgaaagg cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg
     4501 actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg
     4561 aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg
     4621 ggaaggtgca ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc
     4681 gtcgtctctc cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc
     4741 accggattgc cggtatcagt aaggatgacg ggctgataac cctgcaggat gcggaaggta
     4801 acacgcggct gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg
     4861 acaccatccg ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg
     4921 gttatgtggc caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacacttt
     4981 ctgacggtca gcagacccgt gtgattcgcc ccggtcagga gcgggcagag caacatattg
     5041 acctggccta tgccatcacc gcccacggtg cacagggggc gagtgaaacc tttgccatcg
     5101 cgcttgaagg cacggaaggt aaccggaaac tgatggccgg ctttgagtca gcctacgtgg
     5161 ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga taaccgtcag ggctggacgg
     5221 atgccatcaa caatgccgta cagaaaggaa ctgcccacga tgtgtttgag ccgaaaccgg
     5281 accgggaggt catgaatgca gagcggctgt tcagtacggc gcgggagctg cgggacgtgg
     5341 cggcaggccg tgccgttctc cgtcaggcgg ggctggccgg gggagacagt cctgcacggt
     5401 ttattgctcc gggacgtaaa tatccgcaac cgtatgtggc actgccggcg tttgaccgta
     5461 acggcaagtc cgccggtatc tggctgaacc cgctgaccac ggatgacgga aacgggctgc
     5521 ggggattcag cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc gtggccctgc
     5581 agggcagccg taacggagaa agcctgctgg ctgataatat gcaggatggc gtccggattg
     5641 cccgtgataa tcctgacagt ggtgtggtgg taagaatcgc cggtgaaggc cgtccgtgga
     5701 atcccggtgc catcaccggt ggccgcgtgt ggggggatat cccggacaac agcgtccagc
     5761 cgggagccgg aaatggtgaa ccggtcacgg cagaggtact ggcacagcgg caggctgaag
     5821 aggccatccg ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa atggcagaga
     5881 acaaacctga cctgccggac ggcaaaacag agcaggctgt cagggaaatt gccgggcagg
     5941 agcgtgaccg ggctgccata actgaacggg aagccgcgct gccggagagt gtactgcgtg
     6001 aaccacaacg ggtgcgggaa gcggtccggg aagttgcccg ggaaaatttg ctgcaggagc
     6061 gtctgcagca gatggagcgg gatatggttc gtgacctgca gaaagagaaa accccgggcg
     6121 gagactgata caggaagaca aacactgatg acaacggata acacgaacac gacacgtaac
     6181 gattcactgg ctgcacggac cgatacctgg ttgcagtcat ttctggtctg gtcacccgga
     6241 cagcgggata tcatcaaaac ggtggcactg gtgctgatgg tactggacca catcaatctg
     6301 atattccagc tgaagcagga atggatgttt ctggccgggc gaggagcctt tccgcttttt
     6361 gccctggtgt gggggctgaa tctgtcccgt catgcgcata tccggcaacc ggccattaac
     6421 cggctgtggg gatggggaat tattgcccag ttcgcatatt atctggcagg ctttccttgg
     6481 tatgagggga atatcctgtt tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa
     6541 acgcgcagtg ggtggcgtac agcagcggcc atactcctga tggccctgtg ggggcctttg
     6601 tccggcacca gttacggtat tgccgggttg ctgatgctgg cagtcagcaa ccggctgtac
     6661 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     6721 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     6781 accgttggtc tggtgttgtg tgccggaaaa tcattacccc gtttctggcc cggagacttt
     6841 ttcccgacgt tctatgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     6901 tggtatcttt ggccgcaaga ggatgattcg tcagaggcag aacacagcat gacagagcag
     6961 aagcgaccgg tactgacact gaagcggaag acagagggaa cagcgcctgt ccgcagccgg
     7021 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     7081 gagaaagcgg cccgtgaagc agagctggcg gcaaaaaaag cgcaggccag acaggcgctg
     7141 tccatttatc tgaacctgcc cacgctggat gaggctgtga ataccctgaa gccctggtgg
     7201 ccgggattat ttgacggtga cacgccccgg cttctggcct gcggtatccg ggacgtgtta
     7261 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     7321 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     7381 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aggaagcgta tgcgggagcg
     7441 cgtctggcga aaattcgtcg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     7501 gagaaataaa aaaagcctcc cccagagaga ggaggcaggg aaataaggct taaaaggaaa
     7561 taacttccgc ttccgaaagg aagccagtcc actgaattat ttatcacaat aatactgaca
     7621 actaaagggg gacaagaaaa tgaatggatt cagaaacagt tcacggaacg gccaggtctg
     7681 gcgttaccag cgtgccggcg ggcgggccgt tattctggaa gtcagtggac gctggatgga
     7741 agcggcagaa gcatggcgac gggctgcctg tgttgcgcct aggacagact ggcagcagtt
     7801 tgcccggaaa agggctgaac actgccaccg gcgctgccgg ggaagggtgt aacgaaaaaa
     7861 gcagccttca ttactctagg agtcagaagg ctgaaaggag tttatgagat ttgcttctta
     7921 atatccggtc gggcagtatg accggacagt tctgagtagc acagagtctt ttccgcatta
     7981 ag
//
LOCUS       DAETQL010000148         7940 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.150, whole genome shotgun sequence.
ACCESSION   DAETQL010000148 DAETQL010000000
VERSION     DAETQL010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.150"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(52..531)
                     /gene="xdhC"
                     /locus_tag="K0W65_003827"
     CDS             complement(52..531)
                     /gene="xdhC"
                     /locus_tag="K0W65_003827"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HBI3096914.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="K0W65_003828"
     CDS             complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="K0W65_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HBI3096915.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="K0W65_003829"
     CDS             complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="K0W65_003829"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HBI3096916.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4129..4884
                     /locus_tag="K0W65_003830"
     CDS             4129..4884
                     /locus_tag="K0W65_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBI3096917.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4963..5036
                     /locus_tag="K0W65_003831"
     tRNA            4963..5036
                     /locus_tag="K0W65_003831"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4995..4997,aa:Gly,seq:ccc)
     gene            5171..6787
                     /locus_tag="K0W65_003832"
     CDS             5171..6787
                     /locus_tag="K0W65_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBI3096918.1"
                     /translation="MLNSRTYLYQRNGVFYIRLRMKTTGRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKGCQDRLKGDSSGLLSYLE
                     PETSSLRPSVSLSVSSEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEILDYLDIQDVGKATRADMLRVREVLQLLPKNRKQRFKTMPLSDLLNRESKNDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARGAFSPEQVRQLLV
                     AAKAYSQKTAGKPYHYYVTALAAVTGARLNEVAQLQVKDIRVTEAGTVYIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFDGLKLMKNGYG
                     EQVSKWFNRTLIPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVDRLVNVMAGVYKETGVDGDTV"
     gene            6771..7427
                     /locus_tag="K0W65_003833"
     CDS             6771..7427
                     /locus_tag="K0W65_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HBI3096919.1"
                     /translation="MAIPFEAPEFEKEAFNCPYCNAFAHQKWQQLYYYYSSTGGLPVH
                     TSTCEHCEEKSFWFQRRLLIPFASTAELPNPDMPDNCKADYMEARSIVGLSPKGAAAL
                     LRLCLQKLMVHLGEPGNNINADIRSLVQKGLPVRIQQAADICRIVGNQAVHPGEISLD
                     DDPQLAHGLFKLLNIIVDDRITRPKEIEAMFQSMPEGPRQGIENQDRQAREQQQAANE
                     "
BASE COUNT         1987 a         1984 c         2046 g         1923 t
ORIGIN      
        1 ttaaacacat aacctcagtg aatccggcct gaattcaggc cggatatcct tttacttcgt
       61 tttctcgcaa tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt
      121 tcccgccagt ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag
      181 cattgccgtg gtagccataa tcaggccagg cgtacaaaac ccgcactgca ctgtgccgga
      241 tttcgcataa gcctgctgaa catgagaaag ttttccgcct ttcgcttcac cttccagcgt
      301 gcggatctct tttccttcag cccaggcggc aaggtataag caactgtcta ttgctgtgcc
      361 gtcgaccaac accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag
      421 cagtccttgt tcgcggagta attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa
      481 aggcatcccg ttaatggtgc attcggtggt aattgtttcg ctgtgattca ttgcaatttt
      541 ccccccgccg cggcgacggc ttcgctaatc acttttttgg tcatcgtctg gatgagatgc
      601 agacgaaact ctttactggc ccgccatgaa gaacgcgggg cgacatcttg caggacagat
      661 tcgctgatag cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca
      721 tgttggcagc gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa
      781 ttgccgttat ccagtcggca atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg
      841 cgcatggcat atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga
      901 aaggcgacga ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca
      961 ttaatcggga cgaaacgaac accgcgcggg gagtggatct ccagtttcgc gtcataaatt
     1021 agcgttggcg tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc accgtaggta
     1081 gcgacgttac ggatctgcgg tccagcaatg aacgtggccg cagcacataa cgccgggaga
     1141 tgacgttgag ttataggatc ttctattagc tgggtaaatg tcgttgcaga gccgatacgt
     1201 agcgagccat cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca
     1261 atatggcgat aacggtcatt gtgatggtgg agctgaatca gtacgtcagt gccaccggcg
     1321 agcagtttgg cctgcgggtt gtcagccagc aggttgatgg catcggcaag ggttgctgcg
     1381 cgatggtaag aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa
     1441 ctcttcatat aaccgttttg gcgtcagcgg cagtgtattg attgcaacac cggtagccat
     1501 cttcaccgcg ttacgaatag cagcggcaac aggaattatg ggtggctcac ccagtgactt
     1561 atgtccgtat gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag
     1621 atccggcatg gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt
     1681 agcatcgatg atcatctctt caaatagcgc ccagccaatg cccattccca ttccgccgtg
     1741 tacctgacct tctgccagca gtggattaag aatatgccct gaatcatgaa cgttgaggat
     1801 gcggttgatg gtgactttgc acagcgcaat atcgaccgtc agatcaacaa aggtacagcc
     1861 aaacgccggt gggttagtgg tggttttgat ggagctttca gcagagagct gcccgccgcg
     1921 ttcagggtgg tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg
     1981 tcgttcaacc agcacgatat ggccttttat cagggtcaaa ttcatcgctg actgatgtag
     2041 catgactgcg gcgtgagcga tgattttctc ttttaataat agtgccgcac tgcgcagcgc
     2101 aggcgcggca acatagctct ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacgtc
     2161 ggtatcttgt gttgaaataa cgcgaacgtc gctgaccgga acccccacgg tttctgccac
     2221 catttgcgag aagacggtgt cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt
     2281 gatggttcca tcctgattca tcagaaggcg cgcgcctgct atttctacgc cgacaggcca
     2341 ggtgttagag gtgtagctaa aacaggcgac gccaacgccg cggcgcaaat tgccttgctg
     2401 gttctggcat tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca
     2461 ctccggcaac cctgcgctgc aaatacgttt gcccgtgagc ggattagcat ctccttcgcg
     2521 ggcggcgttg cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcgtcatc
     2581 aagcatagac tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc
     2641 cgaggggagg ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag
     2701 gtaagcgact ttattccccc ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc
     2761 ggtgttagac agaacatcca gactataacc tttcaaggtt ccgtcgcggt tcacgcccat
     2821 ttgcccgtca atagtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct
     2881 aagggaaact ttcaccggaa tgccgccaag cttgctggtc aggaatgccg ccattggctc
     2941 ttccagtaca tcctgtttat taccaaaacc gccaccgaca aacggtttga tgactcgtac
     3001 gcatgaccag ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg
     3061 ggtgctggaa acgatggtaa ttcgcgagtc atcctccatc cacgccagcg atgttacgct
     3121 ttccatgtga caatgttgaa taacgggagt ctgatagtgc ccctgtacct ggtagtcggc
     3181 ggcatcgatt gtttgttgga cattgcccgt cgacatcgtg ctttgtttca gtaaattgcc
     3241 gccgttatgg attggtgcag cgtcttctgt cagcgccgct tctggcgtgg tgataacggg
     3301 taattcttgc cactcaatgc tgaccaattg cgccgctttt tctgccgtga gttcatcgcg
     3361 ggccacgacg atggcaacgg cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg
     3421 atcggcggta tcgcgcttgt tttcgtcaag tgtccaggca tgcccagctg tagcgaatgg
     3481 aatatcaggc acatcttccc aagtaaaaat cgccagtacg cccggtaaac ttctggcttg
     3541 ttcatcatta atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcgta
     3601 acacatgccc gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat
     3661 ggcatcgacg cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat
     3721 cccctcgatt tgaacatttg gttcttatgg caaagattta gtctaagcag gcaagattta
     3781 cgccagaaac gacgataagc gttaggggag gaaggaagtg tgatgacgtt aaaaaaacac
     3841 agatatgata cgaaacagga ctctgaatag ggacagtgat acgtatcaat atgagtggcg
     3901 ataaaatcaa tgtgataaat caggattatt acgcatcagg atgggaaata gggtaaaaag
     3961 tcaggaactt cgatacctct acagcgaata ttttgctgat gatacttccc tttgccaaat
     4021 gaaattaacc tctttctcta ttaccacgtt tttccagaag caagagattg ggtatcaaga
     4081 ggctggctgc tatgatatgg cgtcttgttt tttaagcgag gaaagatttt gagtgcggga
     4141 cgcctgaata aaaaatctct gggtatcgtg atgtttttat cggttggact gctattggcg
     4201 ggctgttcgg gtagcaaatc atccgataca ggaacgtatt ccggctccgt ttacaccgtg
     4261 aaacgggggg atacgctata tcgtatttcg cgcaccacgg gaaccagcgt aaaagagctg
     4321 gcgcgactga acggcatttc ccccccttac accattgaag ttggtcagaa actaaaactg
     4381 ggtggggcga aaagtagcag tagtacacgt aaatcaaccg ccaaatcaac gactaaaacc
     4441 gcatcggtta caccgtcatc agcggtaccg aaatcttcct ggccgccagt agggcaacgt
     4501 tgttggttat ggccaacgac agggaaagtt atcatgccgt attcgacagc agatggcggc
     4561 aataaaggga ttgatatctc agctccacgg ggtacaccta tttacgccgc gggtgcagga
     4621 aaggtggtgt atgtgggcaa ccagctgcgt ggctacggta atctcatcat gattaaacac
     4681 agtgaagatt acattacggc ttacgcccat aatgacacga tgctggtaaa taatgggcaa
     4741 agcgtgaagg ctgggcaaaa aatcgccact atggggagca cggatgcggc atctgttcgc
     4801 ctgcatttcc agattcgtta ccgtgcaacg gcaattgatc cgctacgtta cttgccgccg
     4861 cagggcagca agccaaaatg ctgatggcga attaatcagc agtcagcagc gcggcacttg
     4921 ctaaggagag cgtaaggttt ataatgcctt acgcatctcg aagcgggcgt agttcaatgg
     4981 tagaacgaga gcttcccaag ctctatacga gggttcgatt cccttcgccc gctccatgac
     5041 ctttcttaaa gtctactaac ttactgaatc ctaagggaca tggcttaaag ccttccgata
     5101 gttttactca ctgtaaccaa cactgtgact aactttgttg acgtagtgag cagacctaag
     5161 gtctcagtac atgctcaact caagaaccta cctttatcag cgtaacggtg tcttttacat
     5221 ccgtcttagg atgaagacca ctggtcgcct gaccgcttcg cttccttctc acaaccgtta
     5281 taaactagcg tcagtatcac tacggaccaa ggatagacgt accgctatgg ctcactcacg
     5341 gcacatcaag tcagcactta aagcaatcca cgcagataac cctaacgcct cttatgagga
     5401 gctacgggaa cacctgaaga ccattgtcga gtgggaactt agtgtcagcc gcgatgacct
     5461 gaacgacccg gagtcctacc agctttacgt tgaccagtac gatgatatta agtccaacct
     5521 tcgggaagct gtagcgactg agcgtctgac tgtagaccag catcgctata tcaatgacgt
     5581 tatcggcgta cttaaaggct gtcaggatag actcaagggc gatagctctg gcctgttgtc
     5641 ttaccttgag cctgagacca gtagtctaag accatctgtc tccctatctg tatcatcgga
     5701 gcctgaagtc cccgaaccta aagctctgac cctcgcgtct ctcattgagc agtacgagca
     5761 ggagaacgct cagaactgga agccagcgac cctaagcgag aaccgagcgt cgcactccac
     5821 gctaatcgag atattagact atctggatat tcaggatgtc ggcaaggcga cccgtgcaga
     5881 tatgctcagg gtccgtgaag tcctccagct gttaccgaag aaccgcaagc aacgctttaa
     5941 gaccatgccg ctgtctgacc tgctgaaccg ggagtctaag aatgactgtc tagatgtcgt
     6001 taccatcaac aataaatatc ttatcaagat ggctgctgtg tttaagtggg cggtacgcaa
     6061 cgacctgata gctaagaacc tgacagaagg tcttgaactg aaagtccctc agcgtaaagc
     6121 ctccgacgct cgtggcgcgt tcagtcctga gcaggtaagg caactactgg tcgccgctaa
     6181 agcgtactct cagaagaccg caggtaagcc ataccattat tacgtcaccg ctctagctgc
     6241 ggttacaggt gcgagactta acgaggtggc ccagcttcag gtcaaagaca tcagggtcac
     6301 tgaggccggg actgtgtata tccatataaa cgaagacgat agcagcctac cgggtaagag
     6361 catcaagaac gctcatagcg accgctgtgt tccgctagta gatggcgctt atggttttgt
     6421 attggctgac tttatgtcgc ttgtggaaga ccgtagaaag actgagggtg ataatgctat
     6481 ggtattcgat ggcctcaagc tgatgaagaa tggctacggt gagcaggtta gtaaatggtt
     6541 taaccggacg ttgataccta aagtccttgc tgaccgtagc ggcttagcgt ttcattcgtt
     6601 taggcataca gttgcgaccc agttaaaaca acatggggtc gagttagcgt atgctcaggc
     6661 gattatgggt catagctcag ggtctataac ttacgaccgt tacgcgaaag aggtcgaggt
     6721 agatagattg gttaatgtga tggcgggtgt ttataaggag actggagtag atggcgatac
     6781 cgtttgaggc acctgagttt gaaaaagaag cctttaactg cccatattgt aacgcttttg
     6841 cacatcaaaa atggcagcag ttgtactatt actattcaag tactggaggc ctccctgttc
     6901 atacttctac ctgtgagcat tgtgaagaaa aaagtttttg gtttcagcgc cgcttactta
     6961 taccgtttgc ttccacagcc gaactaccaa acccggacat gcccgacaac tgtaaggctg
     7021 actacatgga agccagaagc atcgttggcc tgtcacctaa aggggccgct gcactccttc
     7081 gtctatgcct tcagaagcta atggtccact taggcgaacc ggggaataac attaacgctg
     7141 acatccggtc actcgttcag aaaggactac ctgtacgcat ccagcaggca gcagacatct
     7201 gccgtattgt cggcaatcag gcggtccatc ccggagagat tagccttgat gatgacccgc
     7261 agttggctca tggcctcttt aagctgctga acattattgt cgatgaccga atcacgagac
     7321 cgaaagaaat tgaggctatg ttccagagta tgcctgaagg tccacgccaa ggtattgaga
     7381 atcaggacag acaggcgagg gaacagcagc aggccgctaa cgaataaccc ctcactatag
     7441 gggaagggga cttaactatc actataggga gagaccctaa gtcaccagcc tgctggcatt
     7501 ctccgacaca catatacatt cattaagtct caggcttccg gtagcggacc gattggaaac
     7561 catccgcaga cgcctagtgt taaatcacct ggcctgagac cattctcttt aacgtaggtt
     7621 atcttccctt ttgacaggcg gggagcagaa gaccgcatcc gactttaagt tggctctgcg
     7681 ataatcgctg cgcggataac tgaagcacca agacatcatt gctgaggtct gccatccatt
     7741 tgtggacagg cggaaactca aagtggttga ccagtggagt ctgaggttgg actttgagtc
     7801 ctgaccagca ggctggagga gaccatgagt ttatcttgtt atggattgtg ttatcacaat
     7861 tccaataaga agaactttaa gtccagcctc cagtcctgtc agcccgaagt aaactatggt
     7921 cgctatgata tggtctccat
//
LOCUS       DAETQL010000149         7891 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.151, whole genome shotgun sequence.
ACCESSION   DAETQL010000149 DAETQL010000000
VERSION     DAETQL010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.151"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(150..511)
                     /locus_tag="K0W65_003834"
                     /pseudo
     CDS             complement(150..511)
                     /locus_tag="K0W65_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(850..1404)
                     /locus_tag="K0W65_003835"
                     /pseudo
     CDS             complement(850..1404)
                     /locus_tag="K0W65_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1547..1669
                     /locus_tag="K0W65_003836"
                     /pseudo
     CDS             1547..1669
                     /locus_tag="K0W65_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1931..2497)
                     /gene="ipgB2"
                     /locus_tag="K0W65_003837"
     CDS             complement(1931..2497)
                     /gene="ipgB2"
                     /locus_tag="K0W65_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HBI3096920.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2779..3093)
                     /locus_tag="K0W65_003838"
     CDS             complement(2779..3093)
                     /locus_tag="K0W65_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096921.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3171..3281)
                     /locus_tag="K0W65_003839"
                     /pseudo
     CDS             complement(3171..3281)
                     /locus_tag="K0W65_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3605..4282)
                     /gene="ospD1"
                     /locus_tag="K0W65_003840"
     CDS             complement(3605..4282)
                     /gene="ospD1"
                     /locus_tag="K0W65_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HBI3096922.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4654..4791)
                     /locus_tag="K0W65_003841"
                     /pseudo
     CDS             complement(4654..4791)
                     /locus_tag="K0W65_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4873..5171)
                     /locus_tag="K0W65_003842"
                     /pseudo
     CDS             complement(4873..5171)
                     /locus_tag="K0W65_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5760..5919)
                     /locus_tag="K0W65_003843"
                     /pseudo
     CDS             complement(5760..5919)
                     /locus_tag="K0W65_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5923..7152)
                     /locus_tag="K0W65_003844"
                     /pseudo
     CDS             complement(5923..7152)
                     /locus_tag="K0W65_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7172..7519)
                     /gene="tnpB"
                     /locus_tag="K0W65_003845"
     CDS             complement(7172..7519)
                     /gene="tnpB"
                     /locus_tag="K0W65_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBI3096923.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(7519..>7891)
                     /locus_tag="K0W65_003846"
     CDS             complement(7519..>7891)
                     /locus_tag="K0W65_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3096924.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
BASE COUNT         2026 a         1842 c         1720 g         2303 t
ORIGIN      
        1 ccggctactc attccctgct tgtgcaggat tttaatttcc ataactgtct caaaagtgac
       61 cataagctct cctgaatcag gagagcagat taccccctgg atctgatttc aggcgttggg
      121 tgtggatcac tattgcaccg ttcgtgacag tgttgttgcg ccttgctgcc aacgaagctc
      181 cactgcccat caagttcaca gataagggca acatcagcgt gagcgactgg cgcagatgtt
      241 atccgccggg gcgggagctt tttaatgtcc gtatgacggt attgatgccg attttcagcg
      301 tacggccagt atcgcgaact ccggcaccgt tgaaagccat ttcagtgatt tggtttttaa
      361 ctcccggttt acgggcctcg taacagtaag tgagctgaaa cacccggtga cagtcacggc
      421 aacggaatcg atcatgtcct tttgggttct gcccgtgacg gtaaacctga acggactggc
      481 aacgggggca atgaaagtta actgtgacca tgaaagaacc taaaagcggg tattataacc
      541 cctaattcaa ctaattggag gcattgtcat ttttaatcta tggatacact taaaaatcct
      601 ctcaccttac gttaaaaacc ttctcatgtt tagagttttt tattatgttt atcataatga
      661 tatgacgatc tgagttttta aaatcaagaa gaaaagataa gttttttaaa tgcacagtgc
      721 ctgtggataa ctttgcgtaa atccggagga cagatcactc tgaagcaaga caacagcatc
      781 tgaaaacaat atgctcacgt ttcaaccaca gggcgtaagt gatcctaccc acgtaatatg
      841 gacacaggcc taagcgaggt tctggttttc aaattgttcc ggactgaggc cgccacacca
      901 actgtgccgt cgccagcgat tgtaatcaca ttcgatataa ttaaacactg ttgccctcat
      961 tatttcccgg ctgataaagt gttctccgtg gagacattcc actttcagcg aatgaaagaa
     1021 gctttccacg caggcattat cgtagcagca gccctttgcg ctcatactgc cacgcagatt
     1081 atgccacttc agcagcgcct gataatctgc tgaacagtac tgacttcctc ggtccgagtg
     1141 aacgataacg ttccggggtc tcttacgccg ccacagcgcc atctgcaggg catcgcaggc
     1201 cagttgcgcc gtcaggcgtg gtgacatgga ccagccaata acggcacgtg accacaggtc
     1261 aatgaccaca gccagataca gccagccttc atctgtacgt aagtacgtga tgtctcccgc
     1321 ccacttctgg ttcggaccac tggcgtaaaa atcctgctcc agcagatttt ctgacacagg
     1381 caggccatgt gcgcggtaac tgaccgtatt gctgggtaag atcaatttgc tcactggtat
     1441 ttactacgtg catcgaatga gtgatcaaga gccgtatgac gcgagagtgt cacgtacggt
     1501 tccgtaagca gccggagagt gaaactctct ccggctgact cgactcgcag gttccgacag
     1561 cggtgacgaa catgcagcgg tgttgttctc gctgatcggc acatgccgtc tgaacaatgt
     1621 ggagccagaa aaatggctgc gttacgttat tgaacatatc caggactgac cggcaaaccg
     1681 ggtacgcgat ctgttgcctt ggaaagttga tctgacttct cagtaaatat caatatgatt
     1741 ctgacgagcc gcttaccttt gagcagagaa aggggagagg gatcccacat ccatgagcta
     1801 ggaaaataca aaaactggct atacaacaat tcaatatatt aggacttact tgcgaggaga
     1861 aatatagcac cgctcaggca attcctgggt atttactttt cttagtttct ttactcggga
     1921 tgttctgtca tcagaaaggc gattctaaat ttgtaatata gtcactcata ttcccacgta
     1981 atttctttag atcaatatat tgcacatttt tctcaaaaaa ctgatcggaa ataagatttg
     2041 caacattgct tgttgtatca tttttaacgc ttctattcat cccttcacaa agttcgtcca
     2101 ttttcttttt gaaataactg cttcctgata caagatgatt tatggaactt tgtgcagcat
     2161 tgatgtctag agaaatgcga taatctttct caacctgttt aaatatctgt ttacggagat
     2221 tatcgccgat ttttcggctg tgttctgcac aaaacttatc aatacattgg tttaccaccc
     2281 gggatacgta gacttttttt tcatcatttt tccaatctgt aatattttca ccgatttgtt
     2341 ctgaaacaga gctcaaaata ctagtattta ttttggttga tgatattttt actatccttt
     2401 tccccattgt acagcggata atctcatcca cctttcctga aaaatatctt ttgtgagata
     2461 aactgattcc aaaattatta aaagatgttc caagcattat aatcaccttg tcatgtaaaa
     2521 tagtaacatt gtcattattt tagctgattc aaacaattct gtaaatgctt tgagttcttt
     2581 actcccttgg ctcaatgatg gaccgtctgg aatcccagag actaaactct ctgagaaaga
     2641 gagtatgaaa aaacttgggg cagtccctaa atgaagggac caacagcggt aaaataaccg
     2701 gcatcattca taatgatgcc ctgttagttg tagttatgaa gatttacgtc attaaaattg
     2761 ctgttcgtag tgtcagtcct atggtctggc atcagctgag gatcgtagcc gatacatcac
     2821 tggcttcacg acattcgtgt tgaggctatt catgaaaact ccacgctgaa aacgccgttt
     2881 tgtataaacg gtcacggtat gcccggagcc actgttgctg ataaattcga taaaaccttg
     2941 gcgtttcttg aagctatcgt taatgcggat aatgaaacaa cgattggtga gattcgctct
     3001 tttgccgacg ccctggacgc tgtccggttc aatcgcaaca aaatcaaccg gcagctgagc
     3061 aaacccaacc ttgcctctct ggcgctggag catgaagtga tttggctggg acgtagccgc
     3121 tgacagcttc tataaaagta tgatcctacc cacgtaatat gaacacaggc ttaagtgagg
     3181 ttctggtttt caaattgttc aggactgaga cctccacacc aactgtgccg tcgtcagcga
     3241 ttgtaatcgc acttgatata attaaacact gtatatttca tttgtggagt ctccagtctg
     3301 agagggcttt atgcattccg tattgggcac tatgatgcca gaaatctgca aggctccaca
     3361 ccttcctgta tttcccttcc tctatcacag tgcactttac ttaatagggt gggatgtgtt
     3421 cattacattt attgtgtttt ttatgattgt ttttgcgtcc agtgtcgttc gtcatgggaa
     3481 actggttcta tgggtagtat cgctcagtct agttaactgt ttgtttcttt ttagaaatta
     3541 agcagatcac acaattcggg gagagatcct tctaagtgca attctttgca gctatttagc
     3601 acacttaaaa caactcttgt attttgtcag aaataatatt aaacatgtct tgttcattat
     3661 tgttaagtct gtctgctatt tctgtaagtt ttatcccact atgatggcat atatttatat
     3721 atgccatcat agcctcgata ttccctttcc ctgcggccag aaataatcct ggtaacccct
     3781 cattatctct tacatccaat agtttaatcg tttcttttgg tgataaatca aaagttttaa
     3841 taaaatctcc ataagcttga atcgcagcgg aatgaccttc ttgaagagct atgaacaaag
     3901 cagattcccc agcattattt cgtgctgcaa gaacttctag tttttcctct ttgggtattt
     3961 cggctgccaa cagcgattca ccgtatttac gcgctgcatc ggcatttcca tgccaaatca
     4021 atgaaaacaa attatggcgg ttatactcct tattattctt tgtcggaatg gatatgttct
     4081 gtattttggc caattttctg aaagaaacac cactatatcc cccccctgat ttttcttcat
     4141 tgatggcgtg ggatatagcg gtatttgtca cgttaatgat attatttttc attcctttat
     4201 tttcattagc agtattgctg cctattatta agtgcatatt tttgttgttt gctggatgta
     4261 atccatagtt atttattgac atttttgttc atcctttttg ctaaaaaaaa acaataacct
     4321 atttttgtaa tgtaatagat attagtctat tgctaatcta aaaaaaccat tgccacattg
     4381 aatggctata ttcaccaata aattctttcg atttcgtata ttgacatatc gtagatatat
     4441 cttatcccta agattcaata atcgagtccg tttaccattc tgtgcaagcg ccttcactca
     4501 gaagtgaccg tcaaggcgat caccaaactt gataataaag cagctcattg ccatacgcca
     4561 gtccttaagg ggcgttgtcc atttctgtga tgctgcctag atttaactgc cagctccggc
     4621 ttatctacat ctaaatatgt gagcctaata ccgctacact ttgtccagac tatgtttttc
     4681 tccgggaagt gggctaaccg tctccataaa gtgacgaact tttttcaaca gtggccatat
     4741 tgaacggcac tgatgattac gggttattgt ctcatgaaga gccggccaca tcactgacag
     4801 cagtgacttc aggccttcag caccagacag cctgaaccag ttaatccatc gcccgacaaa
     4861 tttagtaatc tagggctggt ttttcttcag ctgttgcggt tcgaaagtgc cattacgatc
     4921 acgcagagta cggatttcca gaggttcatc tccagtagtg ataatcttcg ttaagcaacc
     4981 attgcgggaa ttgctgccag atctaagctg atttttctcg cacccaagat gatgagtcat
     5041 attagcattg agtacggttt cattttcagc atccaatcaa actgactgag atcttcagga
     5101 tttttgagac ttttggctag ttcattagct aaagcccgca acttattaga tgtcaactgt
     5161 ttttcattca taaattcacc ttcatttgat atcaaattaa atagttcaac atcagagaat
     5221 tatacaaact tatgtacagg ctcctatgat cttcgaatcc tgaagattat agtgaaaata
     5281 agttttcacg ttgaaatccc acatagttag tatatgataa ataatcatgg atgacctgat
     5341 taacctcttg gggtagtgct tttatagaag aataaatatt ctactaattt aatacatcac
     5401 ctaaaatagt attgtttttt atggttggtt gaaggtcgtg gtgatgatgt tagtcctttt
     5461 gagaatattc cagtgccaga tcttacttat aataaagggg cttggcagtt tggataaact
     5521 attgttctta ttccactaat acaaactttt tgtaaagtat attaaatata cttcatttca
     5581 tactgaaatc cccggaagta ttcaatcaaa caattacacc ataaaaggaa gagggggccc
     5641 taattaagtt gtcaagcatg tttatgatac ctgcagggta taaattttct ggctgcaagc
     5701 agacacatat aaaacgtctt tattgatcct acccacataa tatggacaca gtcgtaagtg
     5761 aggctctggt tttcaaattg ttccggactg agaccaccac accaacggtg ccgtcgccag
     5821 cgattgtaat cgcactcgat ataactaaac actgttgccc gcactatttc ccgatggata
     5881 atagtgttct ccgtggagac attccatttt cagcgaatga attttcatct gttgctgtat
     5941 ccagtcatac agtgactgca tcagtggcgc ggctctggct tttcttgccg ccagacgctg
     6001 ttctgctgaa catccccgga cttctgcctc gatggcatac agttcaccga tacgctgcag
     6061 ggcttccgtg gtgatgtcgg ttggcactct tgcatgcaca tcgtggattt ttctccggac
     6121 atgagccata catgcggctt ccgttattct gccggattcg tacaacgccc ggtaaccacc
     6181 gtaagcatcg gcctgaagca ctccgctgta accggccaga tgattctgtg gatggatacc
     6241 tttccggtcc ggtgagtacg cgaaccagac cgccggaggc atttccgaac cggcgttacg
     6301 gtcatcacga acgtagaccc acagccgggc agtccttgtt ttaccgctgc ccggctcctg
     6361 gaccggaacg gggatatcat cggcatggac tttaccgggc atcagcacat actggcgcag
     6421 gatgtcatac agcggttcca gcagttcagc aacagcacct gtccagcgcc ccagtgtggc
     6481 acggctcagc tccacgccct gacgacggta tatttctgac tggcggtata acggcagata
     6541 gtctgcatat tttccagcaa caacatgggc cagaagcccc gctccggcat aactgcgtgc
     6601 aatgggtttt gaaggcaccg gtgcctgcac gatatggtca caccggcaac aggccagttt
     6661 cggacgttgt gtttcgataa ccttaaaggc gctgctgata agctccagct gctctgacac
     6721 atcacatccc agagaactga gttcgccacc acaggccggg cagcattcct cttccggacg
     6781 gatgacccgg gtttcacggg gaagtgaggc aggtaacggt ttacgggctg aagactggcg
     6841 caggggagat ggcagtaccg ggtcatattg ctcacccagc gtttccgcca tttcttcctg
     6901 aagtgcgctg attcgctcct gtgcttccag tatctgccgt tcggtttttg cgcgaagttt
     6961 ttctgagctt ttaccgaact gcatgcgctg cagtttcgca accagcgcct tcagccggtt
     7021 gatttcggaa gcataagccg ccacccgctg tgagagcagg cggttgtatt tcgccatctg
     7081 gcggatggtg tcctgttgcg tctgcaacag tgcccgcagg cgggcattct catgagcaag
     7141 tgaggtgtcc atatcctcac tttacaacgg gttatatgcg gatgccagcg cgttccgttc
     7201 gtttcgggtg tttccagtcg atgccctcaa gaagcatgga taactgggcc ggagtaaggt
     7261 gcaccttgcc gtcacgggtg accggccaga cgaagcggcc ccgctccagg cgtctggtaa
     7321 agaggcacag tccgtcactg tcagcccaca acacttttat ctggtcaccc cggcgtccgc
     7381 ggaagatgaa cagatgcccg gagaacgggt catccttcag gacgttctga acttttgaga
     7441 ccaggccgtt aaagccattt cgcatatcgg tgatacctgc aaccagccag atacgcgaac
     7501 ctgcagggaa agatatcatc agtggctgct cccttttatt tcgcggataa gtatctgtaa
     7561 taacgccggc gtcagtttac ctttaagcct gagagttccg gccggcagaa ccagctcaca
     7621 gcacagactg tcggacggtg tatttatctg ctctggttcc tgtgccggag ccgggatttt
     7681 attatccggc tccggcgtta acgtcacggg aagcagtgca ggcatatttt ttccggaagg
     7741 cagcaggcca cctttccggt attgatggcg ccagttgaag agcaggttat cgttgattcc
     7801 gttttcacgg gcgatctgcg ccacacaggc tccgggctgc agtgactgct ccactaaagc
     7861 gattttaaat tcataaggaa aattaggccg c
//
LOCUS       DAETQL010000150         7746 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.152, whole genome shotgun sequence.
ACCESSION   DAETQL010000150 DAETQL010000000
VERSION     DAETQL010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.152"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1015
                     /gene="ydfJ"
                     /locus_tag="K0W65_003847"
     CDS             <1..1015
                     /gene="ydfJ"
                     /locus_tag="K0W65_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HBI3096925.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1104..2564
                     /locus_tag="K0W65_003848"
     CDS             1104..2564
                     /locus_tag="K0W65_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBI3096926.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="K0W65_003849"
     CDS             complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="K0W65_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBI3096927.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2981..3667)
                     /gene="ydfH"
                     /locus_tag="K0W65_003850"
     CDS             complement(2981..3667)
                     /gene="ydfH"
                     /locus_tag="K0W65_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HBI3096928.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="K0W65_003851"
     CDS             complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="K0W65_003851"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBI3096929.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4639..6684
                     /gene="dcp"
                     /locus_tag="K0W65_003852"
     CDS             4639..6684
                     /gene="dcp"
                     /locus_tag="K0W65_003852"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBI3096930.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6729..7247)
                     /locus_tag="K0W65_003853"
     CDS             complement(6729..7247)
                     /locus_tag="K0W65_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HBI3096931.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7523..>7746
                     /gene="ydeI"
                     /locus_tag="K0W65_003854"
     CDS             7523..>7746
                     /gene="ydeI"
                     /locus_tag="K0W65_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HBI3096932.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKG"
BASE COUNT         1941 a         1813 c         1957 g         2035 t
ORIGIN      
        1 catcttgctg gtgacgctgc gtattattca gggattgggt gcaggtgcgg aaatttccgg
       61 tgccggtacg atgctggcgg aatatgcgcc aaaaggtaag cgcggaatta tctcctcatt
      121 tgtggctatg ggaactaact gcggcacctt aagcgcaacg gcaatctggg cctttatgtt
      181 cttcattctc agtaaagagg aactgctggc gtggggatgg cgtataccgt tcctggcgag
      241 cgttgtcgtg atggtctttg ctatctggtt gcgtatgaat ctgaaagaaa gcccggtctt
      301 tgagaaggtt aacgacagca accaaccgac agcaaaacct gcacctgctg gtagcatgtt
      361 ccagagcaaa tccttctggc tggcaacagg gctgcgtttt ggtcaggcgg gtaactccgg
      421 tttaattcag actttccttg caggctattt agtacagacg ttattgttta acaaagcaat
      481 tccaacagat gcattgatga tcagttcgat tctcggcttt atgaccattc cgttccttgg
      541 ttggttatcc gataaaattg gtcgccggat cccgtatatt attatgaata cctctgcgat
      601 tgtgctggcg tggccaatgc tttctatcat cgtagataaa agctatgccc cgagcaccat
      661 tatggttgca ctgattgtga ttcataactg tgcggtgctg ggattatttg ctctggaaaa
      721 cattaccatg gcagaaatgt tcggctgtaa aaaccgcttc acccggatgg ctatttcaaa
      781 agaaattggt ggtcttatcg cttccggttt tggtcctatc ctggcgggta ttttctgcac
      841 catgacggaa tcctggtatc cgatcgccat tatgatcatg gcatattcag tgattggttt
      901 aatctctgcg ctgaaaatgc cagaggtgaa agaccgtgat ttaagtgcgc tggaagacgc
      961 tgcggaagat caaccgcgtg ttgtaagagc tgcgcaacct tccagaagtc tgtaaaccct
     1021 caatcccttc tcttaccgga gaggggattt ttattcatat aacaaaacat atagcttgcc
     1081 atatttaaat ttaaggaatt gttatgggaa ataatttgtt atcagcaaaa gcgacactcc
     1141 ctgtttatga tcgtaataac ctggccccaa gaattgttca tttaggcttt ggtgcatttc
     1201 accgtgcgca tcagggcgtg tatgccgata ttcttgctac agaacatttc agtgactggg
     1261 gatattatga ggtcaactta atcggcggcg aacagcaaat tgccgattta caacagcaag
     1321 ataatcttta taccgttgcg gaaatgtcgg ccgatgcgtg gacggctcgc gtcgttggcg
     1381 tcgttaaaaa agccttgcac gtacagatgg atggcttaga aaccgtgttg gctgcgatgt
     1441 gtgaaccgca aatcgcgatt gtctctctga caatcaccga aaaagggtat ttccactctc
     1501 cggcgaccgg acagttaatg ctcgagcacc cgatggtcgc tgccgacgtg caaaatcccc
     1561 accagccgaa aacagcaaca ggggtgattg ttgaggcgct ggctcgccgt aaagcggcag
     1621 gacttcccgc atttaccgtc atgtcatgtg acaacatgcc agaaaacggt catgttatgc
     1681 gtgacgttgt cacttcctac gcgcaagccg ttgatgtaaa actggcacaa tggatcgaag
     1741 ataacgtgac tttcccatca acaatggtgg accgtattgt gcccgcagtg acagaggata
     1801 cgctggcgaa aatcgaacaa cttaccggtg tgcgcgatcc tgctggcgtt gcctgtgaac
     1861 ctttccgcca gtgggttatc gaagataact ttgtagccgg acgtccggaa tgggaaaaag
     1921 cgggagccga actggttagt gatgtgctgc cttatgaaga gatgaagttg cgcatgctca
     1981 acggcagtca ttcattcctg gcatatctgg gttatcttgc tggatatcag cacattaatg
     2041 actgtatgga agatgaacat tatcgtcatg cggcgtatgg catgatgttg caggaacaag
     2101 cgccgacgct gaaagtgcag ggcgttgatt tgcaagatta cgctaaccga ttaattgcac
     2161 gctatagcaa cccggcgtta cgtcatcgaa cctggcagat tgcgatggat ggcagccaga
     2221 aattgccaca gcggatgttg gattctgttc gctggcatct ggcgcatgac agcaagttcg
     2281 atctgctggc gctgggcgtc gcgggttgga tgcgttatgt cggtggtgtt gatgaacagg
     2341 gaaatccgat agaaatcagt gacccactgt tacctgttat tcagaaggct gtacaaagta
     2401 gtgccgaagg gaaagcgcgc gtccagtcat tgctggcgat taaggcaatc tttggtgatg
     2461 atttgccaga caatagcttg tttactgcaa aagtgacgga agcgtacttg tctttattag
     2521 cgcatggcgc gaaagcgacc gtggcgaaat attccgtgaa gtaaaaaaag caatgccgcc
     2581 atcggcggca ttcacacact tagttcatgc cgagacgaat caggtccaga ggtgccagtt
     2641 tctcttcaca accttcaaca actacggttt ttccgcggcg gatttgctca gcggtcagac
     2701 cttcagtatc aatcgacagg atcttgccag tgtgaccggt gccgctaacc ataacacggc
     2761 tgccagtggt gattgcgtta cggttacgat cgtaggtcgt cataggtgtt ttctcctttc
     2821 tgatttacag tcgaagccca cgtaacgggc gcaatataaa tactcctgtc gagtgaaata
     2881 ttttttgttt ttgatcaagc tcacatcttt tttgatgcat gcatgagccg taaggcgggg
     2941 ggaaaagcct ctcatcccgg atgggatgag agtaaatgaa ttactcttcg ctaaaccagt
     3001 cgctgttttc ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa tgttgcgtca
     3061 ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc agagaaaata tcaagatgct
     3121 ggcgtaacag catttctggt ggagaaacat ggtcgaaact catatagcgc acgcgatcaa
     3181 cggtcgcttt caggttctca atggtatccc acgccagttg acagtcggca atctgcgtca
     3241 ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg cgctcaatgg
     3301 caatgcgttg ctggtgaaga ttttgttcca gttggtagca ctggctttcg gtaatcatgc
     3361 tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg gataaaactg ccgttgcgca
     3421 cctgggccat ggaaattttg ttgacgtagc tgccacgctg cggacgaatt tgaatcaggc
     3481 cgttttccgc cagtttaata aaggcttcac gaaccggctg gcgtgacaca ttgaaacgaa
     3541 cagaaacttc tttttccgac aacggtgtgc ctggtgcaat caggcaatgg acaatgtcgc
     3601 gacgaagaat acgataaatc tgctgattga caggctgtgt gggattaagt tgcgtttcga
     3661 cggtcattcg ttcttgcttg tgagtgagtt aactgcatga gtctaccact tttttgtgac
     3721 aagcaataac ccggcagtta cgccgggtat aaaaattact gacggtggac attcagtccg
     3781 gcatagcttt gggtaaccgg catcatttcc agggtattga tattgacgtg agcaggcagc
     3841 gttgacaccc accagacggc ttcgctgaca tcttctggcg tcaatgcaac ggtattttga
     3901 taggtttttt ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa ctcggtgcca
     3961 cccaccagac ccggttcgat gtcggtgacg cgcaccgccg taccatgcag atccgtacgc
     4021 agattcaggc taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt accaccggca
     4081 tacggccagc tgcctgccgt tgagccaatg ttaataatat gaccatgatt acgttcaacc
     4141 atacccggta agacggcgcg cgtcatatat actaggcctt tgttgttggt atcaatcatc
     4201 gtttcccagt cttcaacgct ggctttatgc gcaggctcca tgcccaacgc caggccggca
     4261 ttatttacca ggatatcaat attgctccac tcggcaggga gcgatgccag catctcttca
     4321 atagcggcgc ggttgcgaac gtccagttgg gcgatataca gattatctcc cagttcgtct
     4381 gttaactcct gcaaccgctc ctggcgacgg ccagtggcga taactttatg cccttgttga
     4441 ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg ctccagttac taaaacgatc
     4501 atctcactgt tcctcaacgc ttttgtgtgt caataacata gcacgtgatg cttaagagcg
     4561 ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta tggaacacct
     4621 cgcgttcagg agaagaaaat gacaacaatg aatcctttcc ttgtgcaaag cacactgccg
     4681 tatctggctc ctcattttga tcaaattgcc aatcatcact atcgcccggc attcgatgag
     4741 ggaatgcaac aaaagcgggc agaaattgct gccatcgcgc ttaacccgca aacgcctgat
     4801 ttcaacaata ctattctggc actggaacaa agcggagaat taattacccg cgttaccagc
     4861 gtcttttttg cgatgactgc ggcgcatacc aatgatgaat tacagcgtct tgatgaacag
     4921 ttttccgctg aactggcgga actggctaat gatatctatc tgaacggtga attattcgcg
     4981 cgggtagatg ctgtctggca gcgccgtgaa tccctggggc ttgatagtga atccatccgc
     5041 ctggtggagg ttattcatca acgttttgtc cttgccggag ccaaacttgc acaagctgat
     5101 aaagcaaaat taaaagtact gaatacagaa gctgcgaccc tgaccagcca gtttaaccag
     5161 cggttacttg cagcaaataa atccggcggt ctggttgtga acgatatcgc gcagctggca
     5221 ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa aggtctggat
     5281 aacaaatggc tgattccgct gctgaatacc acccaacaac cggcgcttgc cgaaatgcgc
     5341 gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga aaaaaatgat
     5401 gccaatgata cccgcgctat cattcaacgt ctggtagaga ttcgcgcaca gcaggcgaaa
     5461 ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg atcagatggc aaaaacgcca
     5521 gaagcagcac tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg tgcgagcgat
     5581 gaattagcct ccatacaggc ggttatcgat aagcagcagg gcgggtttag cgcgcagccg
     5641 tgggactggg cattttatgc ggaacaggta cggcgggaga aatttgatct tgatgaggcg
     5701 cagctcaagc catattttga attaaacacg gtgttaaatg aaggtgtatt ctggaccgcg
     5761 aatcagctct tcggtattaa gtttgtcgaa cgttttgata ttccggtcta ccatcctgac
     5821 gttcgtgtgt gggaaatttt tgatcataat ggcgtgggac tggcgttatt ttacggtgat
     5881 ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg gcaattttgt tgagcaatca
     5941 acgcttaatg aaacgcatcc ggtaatttat aacgtctgca attatcagaa acccgctgcc
     6001 ggtgagcctg cgttgttact ctgggatgat gtaataacct tattccatga atttggtcat
     6061 acgctgcacg gcctttttgc ccgccagcgt tatgccacgc tttccggcac caacacgccg
     6121 cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca tccgcaggta
     6181 ttcgctcgct acgcccggca ttatcagagc ggggcagcaa tgcctgacga actgcaacag
     6241 aaaatgcgta atgccagcct gttcaacaaa gggtatgaga tgagcgaact gcttagcgcc
     6301 gcacttctcg atatgcgctg gcattgcctg gaagaaaacg aagcaatgca ggatgtcgat
     6361 gattttgaat tgcgggcgct ggtggcggaa aatatggatc ttcctgctat accgccacgc
     6421 tatcgcagca gttatttcgc ccatattttt ggtggcggat atgctgcagg ttattacgct
     6481 tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga gcagggcgga
     6541 ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc tttccagagg taacagcgaa
     6601 gatctggaac gcctgcatcg acaatggcgt ggtaaggctc ctcagattat gccgatgctg
     6661 caacatcgtg gcttgaacat ataaacacca ccaatggctg gcctggagat atcgccaggc
     6721 cagcctggtt atagcagtaa ttgcagcagc tgggcgaggg caaacctcac cgctaaagct
     6781 aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag tgtagttctg gcctttaata
     6841 tgccacgcga accagacggt acccgctggc gtaccatctt caccgccctc cggtccgccg
     6901 tagccggtaa tggcaatact gacatcagca tccgcacgct ctatggcacc tgttgccatt
     6961 tctgctgcca ctttctcact caccgcagaa tatcgttcaa gagactgctg gcttacgctg
     7021 aggattttca tctttgcctg atcggtgaaa gtaacaaagc ctgcaccgta aaatttgggt
     7081 gtatcttcag ctgcacacag ggcgctagcc aacttaccgc cggtgcatga ttcggcggta
     7141 gttaagcgta attgatccgc gataagtctt tgactcaacg cggatgtcag gttttcaata
     7201 gtgtcggtat ctgcgagttg tacaatttta tctttattaa tgttcactgt tttttgtcca
     7261 cgctattata tgtaaaggtg atttgctttt tctgtatgct cgtccaatat aataattttc
     7321 aaacatataa atccgattat tctgaaaaca tgtttaagac tcttcaatct tcttaacttt
     7381 agtgagtcaa attctctgaa tatttgacag ctagtgggct gattttaaat gccttataac
     7441 aattggcagt attcttattt ggcaattata aaaatagcaa ccagacttaa taagtcaaac
     7501 caaacgcaat taaggagtta gtatgaaatt tcagtccatt gtattagcaa gttttcttgt
     7561 catgccttat gcgttggcag atgaccaggg cggtttaaaa caggatgcag cgccaccgcc
     7621 gcctcatgca atagaagatg gctatcgcgg aaccgatgat gcaaaaaaaa tgaccgttga
     7681 tttcgcaaaa aacatgcacg atggcgcttc ggtttcacta cgcggtaatt tgatttctca
     7741 caaagg
//
LOCUS       DAETQL010000151         7723 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.153, whole genome shotgun sequence.
ACCESSION   DAETQL010000151 DAETQL010000000
VERSION     DAETQL010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.153"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..832)
                     /locus_tag="K0W65_003855"
     CDS             complement(<1..832)
                     /locus_tag="K0W65_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HBI3096933.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRPIF
                     E"
     gene            1014..2438
                     /gene="sbcB"
                     /locus_tag="K0W65_003856"
     CDS             1014..2438
                     /gene="sbcB"
                     /locus_tag="K0W65_003856"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBI3096934.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2481..2708)
                     /locus_tag="K0W65_003857"
     CDS             complement(2481..2708)
                     /locus_tag="K0W65_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HBI3096935.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2722..3780)
                     /gene="yeeE"
                     /locus_tag="K0W65_003858"
     CDS             complement(2722..3780)
                     /gene="yeeE"
                     /locus_tag="K0W65_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HBI3096936.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="K0W65_003859"
     CDS             complement(3959..5317)
                     /gene="plaP"
                     /locus_tag="K0W65_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBI3096937.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="K0W65_003860"
     CDS             complement(5307..5369)
                     /gene="yoeI"
                     /locus_tag="K0W65_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBI3096938.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5530..6513)
                     /locus_tag="K0W65_003861"
     CDS             complement(5530..6513)
                     /locus_tag="K0W65_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3096939.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6559..7383)
                     /locus_tag="K0W65_003862"
     CDS             complement(6559..7383)
                     /locus_tag="K0W65_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBI3096940.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="K0W65_003863"
     CDS             complement(7466..7720)
                     /gene="yoeB"
                     /locus_tag="K0W65_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBI3096941.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2068 a         2013 c         1812 g         1830 t
ORIGIN      
        1 gttcgaagat tggacggcgg ataacattac ccatcttgct atgctctttc ggtacggtcc
       61 atacgttgtc cagcaggtca aattctgtct ttgttgccag cctaagctct gagagcctcg
      121 ccccccacag cataagcatc tgatgaagga gcttatttga cgtagacgca cggcttcttt
      181 caatagcaag ccaaatctta gccagttcgt gatacgacag tacccgatcc cctacctcag
      241 cgcgggagcc gaagtccctt ggctggatgc tcataattgc gcaactatct atcaactgac
      301 gccgcatgca ccaactaatt gctgatctta gttgacttag cacctgcctt gctcggcgtg
      361 gattatcttt ttcttcttcg gtaagcaggt caacccattg cttaaccgtg atagaagatg
      421 ccggacgatt agggaaggcg tcatgcatgc gcttcataac cgttgatcga taaagtgcct
      481 gggtcttttc tctgagagtt gtagagacgt agttgtcgaa ccagtagtcg agacactggg
      541 cgaccgtcat ggagttctcc accttctctt caaaataggt gcgtggatcc gttcctgaga
      601 aatagagctt tcgcaagtca gcagtgatct gtctggcatc cttcaaagac agggatgggt
      661 atcgaccaag cccaagtcga ttaggcttgc catgccagcg atagcggtac tggaactgga
      721 tgaccccctt cggtgaaatt cgtacgctga ggccatcggc atcagccact tcttgtgggc
      781 ccgaatatgg tttaccataa atagtacgca gttttgtgtc gcttatagcc ataaataata
      841 tttggtacgc tcagaaataa tagtttggca cacatctggt acacaatatc acatggacaa
      901 cagcgcacag taaacaacta tatttgacgt tcgtagacat actcaggtga aaaaagggtt
      961 gcttttcgag ttataacaga cagttaatta actacagtgg acaatgctag acaatgacag
     1021 acacagataa acaacctacc ttcctctttc acgattacga aacctttggc acgcaccccg
     1081 cgttagatcg ccctgcacag ttcgcagcca ttcgcaccga tgacgaattc aatgtcatcg
     1141 gcgaacccga agtcttttac tgcaagcccg cggatgacta tttaccccgg cctggagcag
     1201 tattaattac cggtattacc ccgcaggagg cacgggcgaa aggagaaaac gaagccgcgt
     1261 ttgccgcccg tattcactcg ctttttaccg taccgaagac ctgtattctg ggctacaaca
     1321 atgtgcgttt cgacgacgaa gtcacacgca acgtttttta tcgtaatttc tacgatcctt
     1381 acgcctggag ctggcagcat gataactcgc gctgggattt actggatgtt atgcgtgcct
     1441 gttatgccct gcgcccggaa ggaataaact ggcctgaaaa tgatgacggt ctaccgagct
     1501 ttcgccttga gcatttaacc aaagcgaatg gtattgaaca tagcaacgcc cacgatgcga
     1561 tggctgatgt gtacgccact attgcgatgg cgaaactggt aaaaacgcgt cagccacgcc
     1621 tgtttgatta tctctttacc catcgtaata aacacaaact gatggcgttg attgatgttc
     1681 cgcagatgaa acccctggct cacgtttccg gaatgtttgg ggcatggcgt ggcaatacca
     1741 gctgggtggc accgctggcg tggcatcctg aaaatcgcaa tgccgtaatt atggcggatt
     1801 tggcaggaga catttcgcca ttactggagc tggatatcga cacattgcgc gagcgtttat
     1861 ataccgcaaa agccgatctt ggcgataacg ccgccgttcc ggttaagctg gtgcatatca
     1921 ataaatgtcc ggtactggcc caggcgaata cgctacgccc ggaagatgcc gaccgactgg
     1981 gaattaatcg tcagcattgc ctcgataacc taaaaattct gcgtgaaaat ccgcaagtgc
     2041 gtgaaaaagt ggtagcgata ttcgcggaag ccgaaccgtt tacaccttca gataatgtgg
     2101 atgcacagct ttataatggc tttttcagtg acgccgatcg tgcagcaatg aaaattgtgc
     2161 tggaaaccga gccgcgtaat ttaccggcac tggatatcac ttttgttgat aaacgaattg
     2221 aaaagttgtt gttcaattat cgggcacgca acttcccggg gacgctggat tatgccgagc
     2281 agcaacgctg gctggagcac cgccaccagg tcttcacgcc agagtttttg cagggttatg
     2341 ctgatgaatt gcagatgctg gtacaacaat atgccgatga caaagagaaa gtggcgctgt
     2401 taaaagcact ttggcagtac gcggaagaga tcgtctaatt tgaagccgcc gccgctggta
     2461 cggcggagga caatgttgag tcaggctttt tgaacggtga tgctccacgc tgcatcgcca
     2521 atctgctgat aatcggtgat ggcatgtcct tcttctgccg cccactgtgg gattgcttca
     2581 gttgcctggg tgcaatcaaa ctcaattacc aactcatcgc cgctggccat ttctgccagt
     2641 gctgcttttg cttctatgag tggaaacgga cagacctgcg tcaccacatc cagcttttta
     2701 atcgccatct ttacacctta cttaatttgc cgcagcagtt gccagtcgcg ccttacgctg
     2761 cggtcgaaca tacacaagcc aggacgcagt ccagactccg agaatcataa atacaaggcc
     2821 aatccagccc tgccaggtca tcattgcagt cataaccagt ccattaccga tagagcaacc
     2881 acctgcaata ctggcaccga agcccatcag tacaccacct aacccgctac gtaatgttgt
     2941 ttgtgcatca gctgcgcgaa cgcggaactc acggctcgct ttggctgcaa taaatgcctc
     3001 cacgaagatc cctaacacca ggaaaacgcc ccagttaatg tatttcacgt cacccgcgac
     3061 cagaaattga agaatattgg ccgttgggga agtgactcct aacccaaaca tgcgtccggt
     3121 tgcttcactc aggggccagg ctaaaagcgc aatcaaaccg atgagtacag cagtgacaaa
     3181 gggatgccag cgtttttcaa acagaatatg agcgatcccg gttcggcgcg gcggtaaggt
     3241 cgcgactttg agttttggct tcttcaactc tttcatcacc acccagagcg ttatcaccag
     3301 caaaacggta accaacggcc acacagacaa attaaaagtc tcagcaatag agttatgttc
     3361 agtagtgtag tgctgcaagg tttgatttaa accactggcg tgtggagaac gcatcaccgc
     3421 actcatcacc atataagtga aaagcgcgat ccaactgccg atcaatccct cacccgcgcg
     3481 ataccaggtc ccggtggcac atccacccgc cagaacaatt cccagcccga aaagataccc
     3541 acctataaca gtaccaagcc acgggaacgc cccggcttcg taagtcagta ggcccgcctg
     3601 aattaacgca aaaaccccca cgctttgtgc cgaaattgca atcagcaagg cgtaaaacat
     3661 gcgattattt ttcgcgatat acatatcgcg aaaaccacca gtcaggcaaa aacgcccacg
     3721 ctgcatgaca aatcccagca gagcaccaca aattagcccg cttaatatca ttgaaaacat
     3781 aaattaatta accagatgaa tgttaatggg gaaatttttc atgactggtg gaatgcagac
     3841 caataaccaa attctctaaa ttagaacaaa tggttattaa tgaggatttc gataaaaaag
     3901 aataaaaaaa ccggagccaa atgttcattc gactccggtt tattattaga aaagatgatt
     3961 atgctacgtc ttcgtactgc ggaaccggat tacggaagct cttggtgacg caagccaggt
     4021 aaatcagacc gatagcagcc cagatcagac ccagaaccat tgagctttct tccaggttaa
     4081 cccacagcgc accaacggtc agcgcaccac acatcggcag gaacagatac tggaagtgat
     4141 ctttcagcgt cttgttacgc ttctcacgga tccagaactg cgagatgacc gacaggttaa
     4201 caaaggtgaa cgccaccagc gcaccaaagt taatcagcgc cgttgccatt accaggtcga
     4261 agttgattgc cagcagggca atcgcgccaa ccaggatgat gttcatcgcc ggagtacgcc
     4321 atttcgggtg tacataaccg aagaagcttt tcgggaacac gccgtcacga cccattacgt
     4381 acatcagacg cgctacgcct gcgtgcgccg ccataccgga cgccagtacg gtaatggtgg
     4441 agaagatcag cgcgccaacc tggaacgctt tacccgcaac gtacagcatg atttcaggct
     4501 gtgacgcatc cggatcttta aagcgagaga tatccgggaa gtacagctgc aggaagtaag
     4561 ttgcaaagat gaagatcatg ccgccaatca gcgcggtcag gaaaatcgca cgcgggatca
     4621 cgcgctctgc atctttggtt tcttccgaca gattgctgat gccgtcaaag ccggtaaagg
     4681 agaagcacag gattgtcgcc ccggtaatca tcgggataac atgcgcatcg ccagaccaga
     4741 acggacgcgt gctcgccagc gtaccagcgc cttcaccttc aaatacgcca taaacaacca
     4801 tgcccagaat caccgcgatc agcactacct gcaacacgac gattacagtg ttgacgttcg
     4861 ctacggattt cagactacgc aggttaaagg cggtcataaa ggccaccagc gccaccacaa
     4921 acatccacga tggaatggaa ggcaccagag cttcaaaata gattttcgcc agcagaatgt
     4981 tgatcatcgg cgcgaacaga tagtcgagca gagaagacca acccaccata aagccgacag
     5041 tcgggctaat ggatttctgg gcgtaagtgt atgcagagcc agcagaagga tagcggcgaa
     5101 ccagcttccc gtagctcaga gccgtaaaca ggatcgcaat caatgcgaac gcataggctg
     5161 tcggcacatg accatccgtg aggcctgaaa cgataccaaa tgtatcaaac agcgtcatcg
     5221 gctgcatata ggctagaccc atcattacaa ccggaactaa cgtaagcgtt ttacgcaatt
     5281 ccacgcgaga ggtgtttgga gtaacgttat gcgacatggt tattctcctt tacggtgata
     5341 accgtcgcgt aagcaaaaaa ttgccccatt tttttggatt cctcagcgac aacaactgtc
     5401 gatttttagt aaatatctat ccggtacgaa gcccggcctc ttggtatgaa tgattggttt
     5461 gaagcaaaaa ataaccgacg ctaattaaac gtcggttttt aatcattttt tgacagcggc
     5521 gcattgtgcc taaatggggg ggaaatgaca agagaatgag aggcttgtca taataatttt
     5581 tctttgaatg gctgattttc cgtcatcaga ttttgcataa acaccggcga tcgcactatt
     5641 tgctaaaatt tcatctcgcc accagccgct ggcaagccct gcggtctgtt cattccagcc
     5701 aatccagaca ggttcaaaga gagtttgggc gaccactttc ttctcgatta acgcgccact
     5761 atcgagaaaa cgttgcgcca gataccgtgg taaatagccg cagccaaggc cgctaatttg
     5821 cagctccagt ttggttttaa aatcgaaaac ggtaatggct tcttgttcgt cgagaagttg
     5881 tgaggccgta gaagcgcctg cctgcgcagt atcccccacc acaatcgccc gatatcgttt
     5941 aatgatacga cggtttaacg gttcttcttc ctgggctaac ggatgatgag gcgcgaccgc
     6001 aaagacttgt tcaagatcgc ccaaccgcga gaaaccaaac tcactggaag atggtggctc
     6061 atgcatcgcg ccaacgataa tatccgctcg cccctgagtc agggcatccc aggaaccacc
     6121 gagtacgcca ttgataaact tcaggcgcgt cacgctatga tgttgataaa aagcttcaat
     6181 gagaggcgca agcagagaaa aaggaaacgt gtcgtccacg ccaatcacca gctcgttttc
     6241 ccagccttca tgcagtttga tcgcctgctt ttccagttct cgcacggtat gcagaacttc
     6301 ccgccctttc tctaataaca tttttccggt gcgggtgaat ttagcgcggt gtccgctacg
     6361 atcaagcaat tgaatattta ggtcgctttc cagcttgtga acggtgtaac tcagagcgga
     6421 cggcgtcttg tagagtttgg ctgacgccgc cgcaaaactg ccctcttttt ctatggcatc
     6481 gaggatcatc aacacatcca gcagtggttt catacacgcc cccttgcagt gcatgcggcg
     6541 tcctgctggc gtgatcgttt actccaacgg cattaccagc ggatcgggat actggtattc
     6601 aaatcccagt tcattacaaa tccgactgcc atcaataatc ttacctttgc cgctgtccag
     6661 actatttctg aactgcggag gttgcagccc cagtaaacgg gccatctgcg gatagaaaac
     6721 attacgcgca gggtgagctg gcgcacatat attatagatg tgtccgcctt taggtgcctg
     6781 taacaacaga gtgatagcgc caataacatc ttctaaatgg actaaattaa caccatgttc
     6841 accatcaggg gcggtttttc cggcaaagaa gcgtcccgga tgacgtcccg gtccgaccag
     6901 gcccgcaaga cgcagaatat cgaccgaagt accgggtaaa ttgtgcagcc agtcttcgag
     6961 ttcttctaat actcgtccac tgttggttac cggattacgc ggggtagttt ctttaaccgt
     7021 gccttgcgcg tcgccataga cagatgttga gctggtaaaa ataattcggg gaatacgatg
     7081 ggccagcgcg ctatccacta actcttgtac cgcttgtaaa tagaactcat cgccggggcc
     7141 gctacgacgt gccggaagcg taatgaccag cgcatcggca tccataaggg catccagatc
     7201 gtcagaatcg caaactaact caggctccat gcgaagcaga tagctatcaa tgccactcat
     7261 tcgggccgct tcgacaccat cttgtgtggt tttactcccg gtgacttgcc agcctcgcgc
     7321 tgaaagtgac atcgccagcg gcatacctaa ccaccctaac ccgacaattg cgaccttttt
     7381 catccgttat ctcctgatac accttagatc tataaggcta cgctagcgta tcaaaactga
     7441 caattcattc tatgaatgaa tctgttcaat aatgataacg acatgctgca atgagcagtg
     7501 aatcatcagt aaccgcgtat accagacggt gctcctctgt aatgcgtcgg gaccagaaac
     7561 ctgacaaatt atgtttcagg ggttctggtt tccccttacc ttcaaatggt gttctgcggg
     7621 tatctttgat aagttcattg atctttttaa caattcgctt atctgtttcc tgccagtaca
     7681 gataatcatc ccatgattcc tcagaccaga ttagtttcac tca
//
LOCUS       DAETQL010000152         7495 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.154, whole genome shotgun sequence.
ACCESSION   DAETQL010000152 DAETQL010000000
VERSION     DAETQL010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.154"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..60)
                     /locus_tag="K0W65_003864"
     CDS             complement(<1..60)
                     /locus_tag="K0W65_003864"
                     /inference="COORDINATES: protein motif:HMM:NF015750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096942.1"
                     /translation="MASISIRCPSCSATEGVVRN"
     gene            171..1661
                     /locus_tag="K0W65_003865"
     CDS             171..1661
                     /locus_tag="K0W65_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBI3096943.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1713..2870
                     /locus_tag="K0W65_003866"
     CDS             1713..2870
                     /locus_tag="K0W65_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBI3096944.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2836..2958)
                     /locus_tag="K0W65_003867"
     CDS             complement(2836..2958)
                     /locus_tag="K0W65_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096945.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3161..4846
                     /locus_tag="K0W65_003868"
                     /pseudo
     CDS             3161..4846
                     /locus_tag="K0W65_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4884..7244
                     /locus_tag="K0W65_003869"
                     /pseudo
     CDS             4884..7244
                     /locus_tag="K0W65_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            7301..>7495
                     /locus_tag="K0W65_003870"
     CDS             7301..>7495
                     /locus_tag="K0W65_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096946.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSL"
BASE COUNT         2204 a         1646 c         1592 g         2053 t
ORIGIN      
        1 gttacgcacc acgccttcag tagcggagca ggaaggacat ctgatggaaa tggaagccac
       61 gcaagcacct taaaatcacc atcatacact aaatcagtaa gttggcagca ttacccttta
      121 cgccgactga atacagtacc aaaggaaagc caaatattat cgtactaacc atggatgatc
      181 ttggttatgg acaacttcct tttgataagg gatcttttga cccaaaaaca atggaaaatc
      241 gtgaagttgt cgatacctac aaaataggga tagataaagc cattgaagct gcacaaaaat
      301 caacgccgac gctcctttca ttaatggatg aaggcgtacg ttttactaac ggctatgtgg
      361 cacacggtgt ttccggcccc tcccgtgccg caataatgac cggtcgagct cccgcccgct
      421 ttggtgtcta ttccaatacc gatgctcagg atggtattcc gctaacagaa actttcttgc
      481 ctgaattatt ccagaatcat ggttattaca ctgcagcagt aggtaaatgg cacttgtcaa
      541 aaatcagtaa tgtgccggta ccggaagata aacaaacgcg tgactatcat gacaacttca
      601 ccacattttc tgcggaagaa tggcaacctc aaaaccgtgg ctttgattac tttatgggat
      661 tccacgctgc aggaacggca tattacaact ccccttcact gttcaaaaat cgtgaacgtg
      721 tccccgcaaa aggttatatc agcgatcagt taaccgatga ggcaattggc gttgttgatc
      781 gtgccaaaac acttgaccag ccttttatgc tttacctggc ttataatgct ccgcacctac
      841 caaatgataa tcctgcaccg gatcaatatc agaagcaatt taataccggt agtcaaacag
      901 cagataacta ctacgcttcc gtttattctg ttgatcaggg tgtaaaacgc attctcgaac
      961 aactgaagaa aaacggacag tatgacaata caattattct ctttacctcc gataatggtg
     1021 cggttatcga tggtcctctg ccgctgaacg gggcgcaaaa aggctataag agtcagacct
     1081 atcctggcgg tactcacacc ccaatgttta tgtggtggaa aggaaaactt caacccggta
     1141 attatgacaa gctgatttcc gcaatggatt tctacccaac agctcttgat gcagccgata
     1201 tcagcattcc aaaagacctt aagctggatg gtgttccctt gctgccctgg ttgcaagata
     1261 agaaacaagg cgagccacat aaaaatctga cctggataac ctcttattct cactggtttg
     1321 atgaggaaaa tattccattc tgggataatt accacaaatt tgttcgccat cagtcagacg
     1381 attacccgca taaccccaac actgaggact taagccaatt ctcttatacg gtgagaaata
     1441 acgattattc gcttgtctat acagtagaaa acaatcagtt aggtctctac aaactgacgg
     1501 atctacagca aaaagataac cttgccgccg ccaatccgca ggtcgttaaa gagatgcaag
     1561 gcgtggtaag agagtttatc gacagcagcc agccaccgct tagcgaggta aatcaggaga
     1621 agtttaacaa tatcaagaaa gcactaagcg aagcgaaata actaaacctt catgcggcgg
     1681 atttttccgc cgccttattg agcgagatag cgatgcacgt tacagccaag ccctccagtt
     1741 ttcaatgtaa tctcaaatgt gattactgtt tttaccttga aaaagagtcg cagtttactc
     1801 atgaaaaatg gatggatgac agcactctga aagagttcat caaacaatat atcgaagcgt
     1861 ctggcaatca ggtctatttt acctggcaag gcggtgaacc cactctggct ggcctggatt
     1921 ttttccgtaa agttattcac tatcaacaac gctatgcagg ccaaaaacgt atttttaata
     1981 cattacaaac gaatggcatt ttattgaata atgaatggtg tgcctttctc aaagaacatg
     2041 aatttctggt tggtatctcg atcgatggcc cccaggagtt acatgaccgt tacagacgca
     2101 gtaattcagg taacggtact tttgcaaaag tgatagcagc catcgagcgt ctgaaatcat
     2161 atcaagtaga gtttaatacg ttaaccgtca ttaataacgt taatgtccat tatcctcttg
     2221 aggtttatca ttttttaaaa tctatcggca gtaaacatat gcaatttatc gaattgctag
     2281 aaaccgggac gccgaatatt gatttcagtg gtcatagtga gaacacattc cgtatcattg
     2341 atttttctgt gcctcccacg gcttatggca agtttatgtc aaccattttt atgcaatggg
     2401 ttaaaaacga tgtgggtgaa attttcattc gtcagtttga aagctttgtc agccgttttt
     2461 tggggaatgg gcatacaagt tgtattttcc aggagtcctg caaggataat ctggttgttg
     2521 aaagtaatgg agacatttac gaatgcgacc attttgtcta tccacagtac aaaattggaa
     2581 acattaataa atctgaactc aaaacgatga acagtgtaca actgacagcg caaaaaaaac
     2641 ggatttcagc gaaatgtcag caatgtgcat ataaacctat ctgcaatggc ggttgtccta
     2701 agcatcgtat tactaaagta aacaatgaga ctgtttctta tttttgcgaa ggttataaaa
     2761 tccttttttc aaccatggta ccttatatga acgccatggt tgagttagct aagaacagag
     2821 taccgcttta ccacattatg gatgttgcaa aacaaatgga gaataattaa taacgctctc
     2881 ttttaactcc attcttagcc tccttcttat gaccttaaca aggcacccga cataaattcc
     2941 tgtcgggtgt ttcgtcatca tcatcttccc ttgcgaaaat cctggcttac gtggtatgcg
     3001 tcagggcatt agcaataccc ttgattatca atagcataac cataaccact gaaaattaaa
     3061 aatttctata tatttcatat tgttaaatga tgcttaaata aatttcatca gtaaatcatt
     3121 gccatagata atcattggat ggcacaatca attttcgcta atgataacca ttcccattac
     3181 gttttgtatg ttgatagcaa agtatctctg tctgctgaag cccttttggc tgcgtaagaa
     3241 taataaaact tcagtgttgt taatcattat tatcctcgca atgatccttg gcgtggttaa
     3301 aattcaggtc tggttaaacg actggaataa tgattttttc aatgcgctaa gccagaaaga
     3361 aaccgataag ctttggcagc tcgttctttg gttccctgcc ctgctgggga tctttgtatt
     3421 aatatccgca aacaaaacat ggttaattaa actactgact attcgctggc gcgaatggtt
     3481 aacagattac taccttaatc gctggtttgc agataaaaac tactacttca cgcagatcta
     3541 cggcgagcat aaaaatacag ataaccctga ccaacgtatc gctgaagaca ttctcttact
     3601 gatcaacaaa acgctcagtc tttcatttgg cttcatccag tcacttagca tgctgatcac
     3661 ctttaccgtt attctttggc aaagcgcggg tacgctctca ttcactgttg gtggaacaga
     3721 atggaatatc caggggtata tggtctatac cgtcgtgctc attgtgatcg gtggaacttt
     3781 atttacccat aaggtgggta aacgtattcg tccgcttaat gtggaaaaac aacgtagcga
     3841 agcgacattt cgaactaatc ttgtgcagca taataagcag gcagaactga ttgcgctaag
     3901 caatgctgag tctctacaac gccaggagtt gcgcgacaat tttcatacca ttaaagagaa
     3961 ctggcatcgt ttaatgaatc gtcagcggtg gcttgattac tggcaaaata tttattcgcg
     4021 ctcgcttagc gttcttccct actttctgtt attgccgcag tttattagcg ggcagatcaa
     4081 tctgggcgga ctgatgaaat cgcgccaggc atttatgcta gtatcgaaca atttaagctg
     4141 gtttatttat aaatatgacg aacttgctga actggctgcg gttatcgatc gcttgtatga
     4201 gttccatcaa ctcactgaat agcgccctac gaataagcct aaaaattgcc aacatgcggt
     4261 acaagtggct aatgcgagta ttcgtacgcc tgataataag atcatattag agaacttgaa
     4321 ctttcatgtt tcgccaggca aatggctatt actgaaaggc tactctggtg cgggaaaaac
     4381 cacactgctt aaaacattat cccactgctg gccgtggttt aaaggtgata tttcttctcc
     4441 tgctgacagt tggtatgtgt cacaaacacc gttaatcaaa agcggcttac tgaaagagat
     4501 tatttgtaaa gcacttcccc tgcccgtaga cgataaatcg ttgagcgaag tactgcatca
     4561 ggttggtctg ggaaaattgg ctgcgcgtat tcatgaccac gatcgctggg gagatattct
     4621 ttccagcggc gaaaaacaac gtatcgccct ggcacgatta attttacgac gtccgaaatg
     4681 gatatttctt gacgaaacta cctctcatct tgaggaacaa gaggctatac gcttactgcg
     4741 tttagtgcgt gaaaaactac ccacaagcgg cgtcattatg gttacacatc aacccggtgt
     4801 ctggaacctg gctgatgata tttgtgacat tagcacggtt ttataacaac aataaaagcc
     4861 ctgctgtacg gagatataaa taaatgaagc gagttcttat tcctggcgtc attttatgtg
     4921 gcgctgatgt ggcgcaggcc gtcgatgaca aaaacatgta catgtatttt tttgaagaga
     4981 tgacggtcta tgctcctgtc cctgtacccg taaacggcaa cacgcattac accagtgaaa
     5041 gcatcgagcg tttaccgacc gggaatggca atatcagcga tctgctgaga accaaccctg
     5101 cggtacgcat ggattcaacg caaagtacct cgttgaacca gggagatatt cgccctgaga
     5161 aaatctctat tcacggtgcg tcgccctacc agaatgccta tttgattgac ggtattagtg
     5221 caactaataa cctgaaccca gcgaatgagt ccgatgccag tagtgcaacc aatattagcg
     5281 ggatgtcaca ggggtattat cttgatgtca gcttactgga caatgtgacg ctttatgaca
     5341 gttttgtgcc ggttgaattt ggtcgcttca atggcggggt aattgatgca aagatcaaac
     5401 gcttcaacgc tgatgatagc aaggtgaaat tgggttatcg cactacgcgt ttggactggt
     5461 taacatcgca tatcgatgag aataacaaga gcgcatttaa tcaaggttct tcaggaagta
     5521 cctatttctc tccagatttt aaaaagaact tttatacctt gtcgtttaat caggagctgg
     5581 ctgataactt tggcgttacc gccggtttat cgcgccgcca gtctgatatc acccgcgcgg
     5641 attatgtttc gaatgacggc attgtcgccg gtcgggcaca gtataaaaac gttatcgata
     5701 ctgcattgag caaatttacc tggtttgcca gcgaccgctt tacccacgat ttaaccttaa
     5761 aatataccgg ctccagccgt gattataata ccagcacctt cccgcagtct gatcgcgaaa
     5821 tgggtaataa atcctatggt ctggcatggg atatggatac gcaactcgca tgggccaaac
     5881 tacgtaccac cgttggttgg gatcatatta gtgattatac ccgtcacgat catgacatct
     5941 ggtacaccga actttcatgt acatatggtg atattacagg gcgttgtacc cgtggcggat
     6001 taggacacat ttcccaggct gtagataatt acaccttcaa aacacgcctg gactggcaaa
     6061 aattcgccgt gggtgatgtt tcgcatcaac cctacttcgg cgcggaatac atctattccg
     6121 atgcatggac tgaacgccat aaccagtctg aatcctatgt gattaatgct gcaggaaaga
     6181 aaactaacca taccatttac cataaaggta aaggcagcct gggaattgac aactacacgc
     6241 tgtatatggc ggatcacatt agctggcgga atgtgtcgtt aatgcccggt gtgcgttatg
     6301 actatgacaa ctatctgtca aaccacaata tctccccgcg ctttatgacg gaatgggata
     6361 tttttgctga tcaaacctca atgattaccg ccggttataa ccgttactat ggcgggaata
     6421 ttcttgatat gggattacgt gatatccgca atagctggac ggaatcggta tcaggtaata
     6481 aaaccctgac gcgttatcag aatttgaaaa cgccttataa cgatgaactg gcaatgggat
     6541 tgcagcaaaa aatcgataag aacgttattg cacgcgcaag tgaagcgcat gatcaaatca
     6601 gcaaaagcag tcgtaccgac agcgcgacta aaaccaccat tactgaatat aacaacgatg
     6661 gcaaaaccaa aacgcattcg tttaacctca gttttgaact ggccgaaccc ctgcatatcc
     6721 gccaggtaga tattaacccg caaattgtct ttagctatat caagagcaag ggcaacttgt
     6781 cgttaaacaa tggttatgag gagagcaata ccggtgataa ccaggtggtt tataacggta
     6841 atctggtctc ttacgatagc gttccagtgg cagattttaa taacccatta aagatctcct
     6901 taaacatgga tttcacgcat caaccgagcg ggttagtgtg ggcgaatacg ctggcctggc
     6961 aagaagcgcg taaagctcgc attatccttg gtaagacaaa tgcgcaatac atcagcgaat
     7021 attcagatta caagcagtat gttgacgaaa aactggatag cagcctgacc tgggacaccc
     7081 gcttgtccta gacgccacaa tttctgaaac aacaaaacct gacgatcagt gccgatattc
     7141 tcaatgtact ggatagcaaa accgctgttg atacaacgaa caccggtgtg gcgacctacg
     7201 ccagtggccg tactttctgg cttgatgtca gcatgaaatt ttaatagttc ggattttact
     7261 taaccgggta aattcgcccg gttttcgcat ggagataatt atgagaaacc tctgtttctt
     7321 actgacgtta gtggcaactc tgttgctccc cgggcggctg attgccgccg ccttaccgca
     7381 ggatgaaaag ttaattaccg ggcaactgga caatgacttg cgatatatga tttatccgca
     7441 tgctcatcca aaggatcagg taaatttatg gctgcaaatt cataccggtt cattg
//
LOCUS       DAETQL010000153         7463 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.155, whole genome shotgun sequence.
ACCESSION   DAETQL010000153 DAETQL010000000
VERSION     DAETQL010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.155"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="K0W65_003871"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="K0W65_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="K0W65_003872"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="K0W65_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBI3096947.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="K0W65_003873"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="K0W65_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="K0W65_003874"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="K0W65_003874"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBI3096948.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="K0W65_003875"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="K0W65_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBI3096949.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="K0W65_003876"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="K0W65_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HBI3096950.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="K0W65_003877"
     CDS             complement(6510..7202)
                     /locus_tag="K0W65_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBI3096951.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1891 a         1809 c         1968 g         1795 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ccggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtgt tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccgggat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagaaaaa gca
//
LOCUS       DAETQL010000154         7387 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.156, whole genome shotgun sequence.
ACCESSION   DAETQL010000154 DAETQL010000000
VERSION     DAETQL010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.156"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..190
                     /locus_tag="K0W65_003878"
     CDS             <1..190
                     /locus_tag="K0W65_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3096952.1"
                     /translation="SLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPHEYNGGLPP
                     NESENRYWKNSNSVASFC"
     gene            complement(214..907)
                     /gene="ospZ"
                     /locus_tag="K0W65_003879"
                     /pseudo
     CDS             complement(214..907)
                     /gene="ospZ"
                     /locus_tag="K0W65_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            963..1115
                     /locus_tag="K0W65_003880"
                     /pseudo
     CDS             963..1115
                     /locus_tag="K0W65_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2496..3443
                     /gene="icsP"
                     /locus_tag="K0W65_003881"
     CDS             2496..3443
                     /gene="icsP"
                     /locus_tag="K0W65_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HBI3096953.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(4005..4377)
                     /locus_tag="K0W65_003882"
                     /pseudo
     CDS             complement(4005..4377)
                     /locus_tag="K0W65_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4621..4740
                     /locus_tag="K0W65_003883"
                     /pseudo
     CDS             4621..4740
                     /locus_tag="K0W65_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4990..5856
                     /gene="ospB"
                     /locus_tag="K0W65_003884"
     CDS             4990..5856
                     /gene="ospB"
                     /locus_tag="K0W65_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HBI3096954.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            6185..6925
                     /gene="phoN2"
                     /locus_tag="K0W65_003885"
     CDS             6185..6925
                     /gene="phoN2"
                     /locus_tag="K0W65_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HBI3096955.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7285..>7387)
                     /locus_tag="K0W65_003886"
     CDS             complement(7285..>7387)
                     /locus_tag="K0W65_003886"
                     /inference="COORDINATES: protein motif:HMM:NF017898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HBI3096956.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2310 a         1402 c         1436 g         2239 t
ORIGIN      
        1 gagtctgaag aacgaatgga tgccggtggt gggttacgta agcttcagcg aggcagctca
       61 cgccataacg gactatatcg ttggatatta cagcgcacta agaccgcacg aatataacgg
      121 tgggttaccc ccaaacgaat cggaaaatcg atactggaaa aactctaact cggtggccag
      181 tttttgttga ccacttcata caattaatat acattaagta acaggcattc gagccttgat
      241 ttgagaaagc ctgtcattca tttgttttat gtagctatct atttcctctg atgcataact
      301 aaaaagttct cccatttcac gagcaataat aatctcagat ttaatagact ttaatctctg
      361 gcgactcatc tcttaacact tgcataatct ctctttttat atcatatagt tctataaacc
      421 gatttgcccg tagttctcct tgggaagaat catttctgaa tacaacaatt atatacaaaa
      481 aatcactatt ttctttagag aagatcttac aactaatctc tgggagctca cataatgcat
      541 gtaagtcagg atggtacaaa ctatccacag aaaattgccc cataggacgt tttccagata
      601 taactgtcaa ccagcttcct tccggtgtac tggcttcggc tctggaaata ggctgaaaat
      661 cttccggaca tggagaatac attgtcttca tataattaag tcgtctggat ttctcattaa
      721 taaactcctc tatttttaat tgtgccatac ttctggttat accagagata cacttcttgt
      781 catatatgtt tggtgattca ttaaagtaac catgttcaac tctaggataa atatttctca
      841 ctatatattc agtatttgca gtatttattg gaaatacgtt cttaatattc ttgatgggac
      901 taatcatttt aatctctata ctcaaatata aacattacca tgaacaatat aattgttcct
      961 aagtggaatg tctccacgga gaacacttta tctatcggga aatagtgcgg gcaacagtgt
     1021 ttaattatat cgagtgcaat tacaatcgct ggcgacggca ccgttggtgt ggcggcctca
     1081 gtccggaaca atttgaaaac cagaacctcg cttaggcctg tgtccatatt acgtgggtag
     1141 gctcacagag cctcacttac gactgcgtcc atattatgtg ggtaggatca ataaagacgt
     1201 tttatatgtg tctgcttgca gccagaaaat ttataccctg caggtatcat aaacatgctt
     1261 gataacttaa ttggggctcc ctcttccttt tatggtgtaa ttgtttgatt gaatacttcc
     1321 ggggatttca gtatgaaatg aagtatattt aatatacttt acaaaaagtt tgtattagtg
     1381 gaataaggac aatagcttat ccaaactgcc aagcctcttt attataagta agatctggca
     1441 ctggaatatt ctcaaaagga ctaacatcat caccacgacc ttcaaccaac cattttattg
     1501 catttaaatc ccagaatagt tcagtagtgg tcaatttggg aaacgcacaa tattgtacgg
     1561 ttacgttatc gcaatagagt gaactctcgc cctatacggc gttttcaagg attagtcctt
     1621 aatcggacaa ccacattaaa gcataataaa taattaagct ttaataatat actatagata
     1681 cagttctatt ataaaaaata ctattttagg tgatgcatta aattagtaga atatttattc
     1741 ttttataaaa gcaccaatga attgtccaag taaacatgag ataaaatgtg tagaggataa
     1801 ctgagtttgg tcactttaac tgtattagtc gcagtctgac tctcgacttt aaaaggatgg
     1861 gaattacctg tactgatagt gagaaagctt tccattgaaa taagtcaaat aaaagttgat
     1921 ggcttcagtg ctaaagtgag gtgcccgcta gccgtattca ctcgaatcgg aattcaatgt
     1981 attgcttctg ccatttccat aatatttcac gggaaaaaca aaatctgaaa aactatactt
     2041 aagtgttgta ttgagtcagt tgagatacaa atacatattt atcagcacac gttacgtcaa
     2101 atacaggagg ttgtcatgtt ggtactgaaa ggcacgttgg ctattgcagc cgtgatgaca
     2161 atttattgtc ttatcgttgt tcttatggaa cgcttttctg attgatttca tattggcgag
     2221 agaacaagaa ggtgagtaga attgtcgcgg aatcctgaaa ctatcagcct caggtatgaa
     2281 caccaaaagg taggtagaga aatctatggc ccccgttact ttattcgcac aatgaggtat
     2341 ttttttattt ttacaaaagt tcaataaata ttataataaa agatatgttc ttggtaacat
     2401 aaacaaatat ttaattaaaa taatgcttgg cattgacgtt tatcttataa agtaagaaga
     2461 tcatggacat ttcaaccaaa aaagtagagt tctcgatgaa attaaaattc tttgtacttg
     2521 cactttgtgt acctgcgatc tttactacac atgctaccac taactatcca cttttcatac
     2581 cggacaacat cagtacggat attagtctag gatctctgag tggcaaaaca aaagaacgcg
     2641 tttatcatcc caaggaaggg gggagaaaaa ttagtcaact ggactggaaa tacagtaatg
     2701 cgactattgt tagaggtggc atcgattgga agctaattcc aaaagtgtct ttcggagttt
     2761 ccggttggac tactttaggt aaccagaaag caagcatggt tgataaagac tggaacaatt
     2821 ccaatacccc tcaggtatgg accgaccaga gctggcatcc caatacgcat ctccgtgatg
     2881 ctaacgaatt cgagctgaat cttaaaggtt ggttattaaa taatttggat tatcgacttg
     2941 gactaatagc aggttaccaa gagagtcgtt acagttttaa tgcaatggga gggagttata
     3001 tttatagtga aaacgggggg agcaggaata aaaaaggggc acatcctagt ggtgaaagaa
     3061 caataggtta caaacagctc ttcaaaatac cttatattgg attaactgct aattaccgcc
     3121 atgagaattt tgagtttgga gcagaactga aatatagtgg ttgggttctt tcatctgata
     3181 cagataaaca ctatcagact gagacaattt ttaaagatga aataaaaaac caaaattact
     3241 gctctgttgc tgcgaatatt ggatactatg tcacccccag tgcaaaattt tatatagaag
     3301 gctccagaaa ttacatttct aataaaaaag gtgatacatc tctttatgag caaagtacca
     3361 atatatctgg caccattaaa aatagtgcaa gtattgaata tattggtttt ctcacttccg
     3421 caggtataaa gtatattttt tgaattggtt caccgagttg attacgtttt catggctaaa
     3481 aattgccgcc cattgtgagt catattcgcc ctgactttcc agaaccatac gaactgcccg
     3541 ttgacggact tcgggggaaa aacgtgtatt tttagtcatt ctgtttacct ctttctcagg
     3601 gcgtttagtc tccaggattt ccggggcggt tcaaaaaact cttcgttgat tactgtggac
     3661 ttactgttgg cattaccgac cctgagaccg gagaaataag aaccgcttag atcatcgtgg
     3721 ctgttctcgg ggtatcaagt tacacatggg cagaggctac ccaaggtcta ctattatcgc
     3781 ctgaaagcgc tgatggaaca gtgctatcag ggacatgctg atgaaaatac ctaaataatc
     3841 agccagcact ctatctttcc aaatccacag catagcaaag agagcaaaag agcctgtaaa
     3901 ttcagaaatt aaggaaacgc ctcttggtgt acataatcta tcttatgtta aatgaaatct
     3961 aatttcttga ttatatgctg gatcgttcgt gggtggcttt gcccctatat ttccagacac
     4021 ctgttatcat tggcccgctg ccgcagatat tcccgtggcg agcgataatc cagcgcacta
     4081 tgcggatgcc attcgttata atgctcgaat gcctctgcaa ggttctttgc tgccgttaac
     4141 ccgtcaggtt tgggcatgat actgatgtag tcacacttta ttgttttcac gaagctcttt
     4201 gctattctgt tactctccgg actccgcacc gccgtgttct tcggttcaac aaagtcccaa
     4261 catccgggtg aactggcgtg tttcattagc tcggtagcat gaaccattat ccgtcagtca
     4321 cttcactgga gacgccggaa gctcgttgcc gaagcggtgt tccaccgctc ccagcatgtc
     4381 gccaacggga gagcatctac ggtctgattg aactgtttat tcaaattgtc caccgtctgt
     4441 cagtacgctc tgaacgtcgg ctggttaaaa cactgctcgc tgattttcag agggttcacg
     4501 gtaaaacagc acttttattc cggattgcgg aagcggctct gaataatccg gatgggctgg
     4561 ttaaagaggt tgtgtaccat ctgcatattg ttgaaccaga tcgcagcggt aagagaagtt
     4621 catcatactt ggcgcagtta cgcgatgttt ctgctagagg tgatgccgta aagaacggca
     4681 gaacattacc ggaacaagat agcggtctac ctgcactggt atcagacccg gggcttcccc
     4741 ggatgatctc cacggtactt taggtactgt gtctatatta tgtaggtagg atcaattaac
     4801 tttgtagttg aaattctttc attgaaataa tgaataaaaa agacaaaccg cattaaacaa
     4861 tgccagaata taattgcatt tttgttccgt ttttttaaat catagtatac aaagaagctt
     4921 gcatactata tgaaaatata acacccttta ctatgatgtt tataaaacaa tatatggagt
     4981 catgtaggta tgaatttaga tggtgttaga ccatactgta gaatagtcaa taaaaagaat
     5041 gaaagcatat cagatattgc atttgcacat ataataaaaa gggtaaaaaa ttcatcatgt
     5101 actcacccaa aagcagcatt ggttttttta ggagagaaag gtttttgtga tagcaatgat
     5161 gttctatcta ttatgggaca acaaatacca agagtattta agaacaagat gttatatgat
     5221 tatgttttta aaaatgaaaa aagtaaaaat gattttctaa aaatggctga atcatggcta
     5281 ccacagagtg aaccaatagt aataaataat gatgatgacg cattgaatgc tgctgcttat
     5341 ttttctgtaa aaaaagcgaa aataaaaaca gtaaacgata ctgattttaa agagtataat
     5401 aaggtttata ttcttgggca cggtagtcct ggttctcatc aattaggcct tggttcggaa
     5461 cttattgatg tacaaacaat catttcaaga atgaaagact gtggtattct aaatgtgaaa
     5521 gatatccgtt ttacttcatg cggctccgct gataaagtgg ctcctaaaaa ttttaacaat
     5581 gcccctgctg aaagtctttc ttgtatcctt aactctctgc ctttttttaa ggaaaaagaa
     5641 tctttgctag agcagataaa aaaacacctt gaaaacgatg agtcattgag tgatggtcta
     5701 aaaatatccg gctatcatgg atatggagtt cactatggtc aagagctttt tccctactca
     5761 cattatcgtt caacttcaat tcctgctgat ccggagcata cagtaaaaag aagctctcag
     5821 aaaaagactt ttattattaa taaagaactg gattagtata aaatttttaa cctatagtta
     5881 attgtttgca tttaagatga agttatttta tctttagtag aaaagtgctt ccctatgagg
     5941 gaaaatcaca aatcatcata atacagagac aaaacgatac gaaaaaatag ataaaaaaca
     6001 tcattctttt accacattat tttccgtgaa tatgaaaaat aatgttatta ctttaatata
     6061 agactatttt ttgtttttcc atcactctgt tcaaattttt ccgcatgact tgtgtttttt
     6121 gtaatacagc tcgtttttta cagctgacca aaatcatcaa ttaattatgc taaggaaata
     6181 aattatgaaa accaaaaact ttcttctttt ttgtattgct acaaatatga tttttatccc
     6241 ctcagcaaat gctctgaagg cagaaggttt tctcactcaa caaacttcac cagacagttt
     6301 gtcaatactt ccgccgcctc cggcagagaa ttcagtagta tttcaggctg acaaagctca
     6361 ttatgaattc ggccgctcgc tccgggatgc taatcgtgta cgtctcgcta gcgaagatgc
     6421 atactacgag aattttggtc ttgcattttc agatgcttat ggcatggata tttcaaggga
     6481 aaatacccca atcttatatc agttgttaac acaagtacta caggatagcc atgattacgc
     6541 cgtgcgtaac gccaaagaat attataaaag agttcgtcca ttcgttattt ataaagacgc
     6601 aacctgtaca cctgataaag atgagaaaat ggctatcact ggctcttatc cctctggtca
     6661 tgcatccttt ggttgggcag tagcactgat acttgcggag attaatcctc aacgtaaagc
     6721 ggaaatactt cgacgtggat atgagtttgg agaaagtcgg gtcatctgcg gtgcgcattg
     6781 gcaaagcgat gtagaggctg ggcgtttaat gggagcatcg gttgttgcag tacttcataa
     6841 tacacctgaa tttaccaaaa gccttagcga agccaaaaaa gagtttgaag aattaaatac
     6901 tcctaccaat gaactgaccc cataaagctg gacagcctgt atcaggctat ggagggccca
     6961 tagacaaatc taccctatat gagcatagga ggagtctatg ggcacaccac gttttacccc
     7021 tgaatttaag ggattactgg aaaggctggg acatatccct ccggcagaag cagaaaaagc
     7081 ttattatgct gccatcggaa acgatgatct ggcaacctga gttcacagat aaaacattct
     7141 ctaggaaact cggggcggtt ccgttcacca catgcaatgt ggtgttgcag gggaacggtc
     7201 tgcaccatcc cctatgtcga tcaatataac agaaatgaca acttcagatt cagggcacaa
     7261 cctaaatata ttttaggtca cctctcaaat cgtttgcctg ataccgctcc attttttaat
     7321 aaaaaaatca atcatttttg aatctttgct tttcattgca ttgtcaagca tagtgtcccc
     7381 actattt
//
LOCUS       DAETQL010000155         7268 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.157, whole genome shotgun sequence.
ACCESSION   DAETQL010000155 DAETQL010000000
VERSION     DAETQL010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.157"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            18..1076
                     /locus_tag="K0W65_003887"
     CDS             18..1076
                     /locus_tag="K0W65_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBI3096957.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
     gene            1119..1622
                     /gene="yeaK"
                     /locus_tag="K0W65_003888"
     CDS             1119..1622
                     /gene="yeaK"
                     /locus_tag="K0W65_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HBI3096958.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1623..1727)
                     /locus_tag="K0W65_003889"
     CDS             complement(1623..1727)
                     /locus_tag="K0W65_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096959.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1897..2343
                     /locus_tag="K0W65_003890"
     CDS             1897..2343
                     /locus_tag="K0W65_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBI3096960.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="K0W65_003891"
                     /pseudo
     CDS             complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="K0W65_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3215..4395
                     /gene="nimT"
                     /locus_tag="K0W65_003892"
                     /pseudo
     CDS             3215..4395
                     /gene="nimT"
                     /locus_tag="K0W65_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4450..4797
                     /locus_tag="K0W65_003893"
     CDS             4450..4797
                     /locus_tag="K0W65_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBI3096961.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="K0W65_003894"
     CDS             complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="K0W65_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HBI3096962.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5256..6281
                     /gene="dgcP"
                     /locus_tag="K0W65_003895"
     CDS             5256..6281
                     /gene="dgcP"
                     /locus_tag="K0W65_003895"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HBI3096963.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6314..6412
                     /locus_tag="K0W65_003896"
     CDS             6314..6412
                     /locus_tag="K0W65_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HBI3096964.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6415..6489
                     /gene="yoaJ"
                     /locus_tag="K0W65_003897"
     CDS             6415..6489
                     /gene="yoaJ"
                     /locus_tag="K0W65_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HBI3096965.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6650..6841)
                     /locus_tag="K0W65_003898"
                     /pseudo
     CDS             complement(6650..6841)
                     /locus_tag="K0W65_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004995861.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7021..7137)
                     /locus_tag="K0W65_003899"
     CDS             complement(7021..7137)
                     /locus_tag="K0W65_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3096966.1"
                     /translation="MRNVFRCHIPRYEAIIARYLDMEPAQIWPSRYMEKTSC"
BASE COUNT         1753 a         1763 c         1770 g         1982 t
ORIGIN      
        1 ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt
       61 ctgccaataa tacccatggc ttaaacctga ctgcccataa atacccgggc ttacgcggca
      121 cactacaaac cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg
      181 cttttgatca ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga
      241 tggccgaaga gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg
      301 agccggttaa tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta
      361 ttggcatcga tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca
      421 tttaccagga tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtatatgtcg
      481 cagggcagct aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct
      541 tttacactca tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg
      601 attcggggcg cgacattatc atcaaccaga gcgaagataa tctgttccag tatgtcagtt
      661 acgtccatga cttacctggc ggcattcgtg tctcgttatc cattgatatt ctttacttta
      721 tcacgtcttc gaggaaaagc gttctgttct ggattttgac ggcgttaatt ttgctgaata
      781 tggtccggat gcacttccgt ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga
      841 tgactggact gtataatcgc aaaattttaa cccctgaact ggagcagcgg ttgcagaaac
      901 tggtgcaatc cggttcttcg gtgatgttta ttgcaattga catggacaag ttaaagcaaa
      961 taaatgacac cctcggtcat caggaggggg atttagcgat tacgttacat gatgcgtata
     1021 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
     1081 cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg gctaaaggaa
     1141 gcgtgacgca tcagcagtta atcgctttat tatcacaaga aggtgctaac ttccgcgtcg
     1201 ttacccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggt accgctctag
     1261 gccagggtgc taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc
     1321 tggcgatcct cgcagccgat caacaggccg atctgagcca actcgccagc catataggcg
     1381 gattacgcgc ttcgttggcc agcccggcgg aagttgatga actcacaggc tgtgtcttcg
     1441 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
     1501 tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc gttattttga
     1561 aaaccgcgga ttatttgcgc attgcgcaac cagaactggt caatttccgc cgcactgcgt
     1621 agctagccta tccgttcaat aagcagaacg gacaaaacca gcacaaccgc aatagcaaaa
     1681 aacgatgatg tgataatcag tgtctcgacg aacatttgat cgttcatagc atgcccccgg
     1741 gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttcgc
     1801 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcataaag
     1861 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
     1921 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
     1981 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
     2041 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
     2101 gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc gctggtggca
     2161 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacgtt aatgggcagc
     2221 cagccgcaac tggttgctgg gctgctggtg ggcactgttt taggcgtagc gctgtttcgc
     2281 ggcgtaccgg ttggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
     2341 tagttaatcc cgcaatatat cggcctggcg tttgccccaa gcctttctta aacatagtaa
     2401 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
     2461 tcactaatcc ctgaagcgcc ataatcagct gtaactgctg acgccactgg cgaaaactca
     2521 accccgtctc tttcactatc aggcgggcaa gattgcgttc actcatcgca aaaaagccag
     2581 cccattgccc caataccccc cactcgacgg gccctttcgc catcatctcc accatcgtgc
     2641 ggattttagg atgagaagaa acgggcaggt gcagctgttg ttgtggctgc tgagggagtt
     2701 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
     2761 tgtttgccag ggttaaaatc aattcccggc ataagggcga tattttcaat gtgcaacatg
     2821 gtcggcattg tcactgcgct gggttcgata aacagaaaac agagttcagc gttagctgtc
     2881 acttgattgc tatgcttcac cccgcccggt atccaaaccg catattgtgg cggcaccatc
     2941 cacaaagcat tttccaccgt acaggtaatt gcaccatgta atgccagtat cagctgtcct
     3001 ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat acaaaatgcg
     3061 accgcggctt catggtgtcg gtgggttcat agccattgag attcaacctg tgcatcattt
     3121 tgtccgaact tagcgataat ttgtcatttt agcttgattc aacataacaa taaaaacggt
     3181 aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt cattaagcgg
     3241 caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat tacgcgtcac
     3301 ctttaccggc gcagcgccgt tactggatac gattcgttcc gcttactcgc tgacgacagc
     3361 gcaaaccggc ttattaacca ccctgccatt attggccttt gcgctaatct cacctctggc
     3421 tgccccggtg gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt tacttttgat
     3481 ctgtgcaggt atcgcaattc gttctctccc ttcgccttac ttattatttg gcggtacagc
     3541 ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa ttaaacgcga
     3601 tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg gagctgcagc
     3661 ggcactggga tcggctatgg tcgtgccgct ggctttgaac ggttttggct ggcaaggcgc
     3721 gttgttcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc cacagtggcg
     3781 aagtcagcaa catgcaaatt tgagtacctc acgcgcctta catactcggg gtatctggcg
     3841 ttcgccactt gcctggcagg taacattgtt tcttgggatc aactcactgg tctattacgt
     3901 gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag cacaggcggg
     3961 ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga tcgcactttt
     4021 cttacatcat gtgaaagacc agcgtggtat tgcagcgttc gttgccttga tgtgcgcagt
     4081 gggcgcggtt gggctctgct ttatgccagc gcacgcgatc acctggactc tgcttttcgg
     4141 ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc ggcaagttct
     4201 gcgcatcagg cggcggcact ctcggggatg gcacaatccg tcgggtattt gctggcagcc
     4261 tgtgggccgc cgctaatggg taaaatacac gatgctaacg gtaactggtc cgtaccactg
     4321 atgggtgttg caatactttc actactgatg gcgattttcg gactttgcgc cgggagagac
     4381 aaagaaattc gctaatatcc ggtgctatag tgacgtgaca aatcttgcgt gaaagggaga
     4441 acaaacacga tgaatattca gtgcaaacgc gtttatgatc cggctgaaca gagcgatggt
     4501 tatcgcgtac tggtcgaccg cctctggccg cgcggtatca aaaaaaccga tttagccctt
     4561 gatgagtggg ataaagaaat cacgccgtca acggaactgc gcaaagcctt tcacggcgaa
     4621 gtcgtcgatt ttgcaacctt tcgcgagcaa tatcgcgcag aactggcgca acacgagcaa
     4681 gaaggaaagc ggctggcgga catcgccaaa aaacagccgc tgaccctgct ctacgcagca
     4741 aaaaacacca cgcagaacca tgcgctggtg ctggccgact ggctacgtag cttgtgattt
     4801 tagtacggca tccggcggtt atttttcacc agccggatgg tcccgtcgcc acaatgccca
     4861 ttcatcaatc gtttcaccgc ccggtaattt gcaattgttg ctgacccctt gcgctgtctg
     4921 cactggaatg agcgtcccgc ccttctgctg gcaatagacc gacgccggat ttgccatacc
     4981 aatctgcggc ggtttaggtg cttctggctg agaaggggtt gaacaaccag ccaggaccag
     5041 caagcaaggc agaacaaaac tgataatttt catttattga tctcacatat ttatccaagc
     5101 ttagagtatc gcggtatcgt tttgttttgc agcactattt ttattacatt cactcaaaac
     5161 atattacgtc ttgtttcatc tttgttgatg atgttttatc atgcctgcaa agattaaata
     5221 atcagcactt acccgccgta tcctggagtt gttccgtgtc agatcagatt atcgcccgcg
     5281 tctcgcaatc ccttgccaaa gaacagtcac tggaaagtct ggtccgacag cttctggaga
     5341 tgctggaaat ggtcactgat atggaatcaa cctacctgac caaagtggat gtcgaagcgc
     5401 gcctgcagca tattatgttt gcccgtaaca gccagaaaat gcacatcccg gagaatttta
     5461 ccgtcttgtg ggattactcg ttatgcaaac gcgccattga tgaaaactgc tttttcagcg
     5521 atgaagtccc tgaccgttgg ggcgactgta ttgcggcacg caatcttggc atcaccacat
     5581 ttctgagcac gccaattcac ttaccggatg gatcattcta tggcacgctt tgcgccgcca
     5641 gcagtgagaa gcgccagtgg agtgaacgcg cggaacaggt tttacagtta ttcgccggac
     5701 tgattgcaca atatattcaa aaagaggcac tggttgaaca gctgcacgaa gccaatgctg
     5761 cgctgattgc gcaatcgtat accgactcgt taaccgggct accgaatcgg cgggcgattt
     5821 ttgaaaatct gacgacgctg ttttccctcg cccggcatct taaccataag ataatgatcg
     5881 cgtttatcga tctggataac ttcaaattaa tcaatgatcg ttttggtcat aatagtggcg
     5941 atctgtttct cattcaggtt ggcgagcgcc ttaatacgct ccagcaaaat ggcgaagtta
     6001 ttggtcgtct cggtggtgat gagtttttgg ttgtttcact aaacaacgag aatgcggata
     6061 tttcgtcgct gcgagaacgt attcaacagc aaatacgtgg agaatatcac ttaggtgatg
     6121 ttgatttgta ttatcccggt gccagtcttg gcatagtaga agtcgatcct gaaacaaccg
     6181 atgcagacag tgccctgcat gctgccgata ttgcgatgta tcaggagaaa aaacacaaac
     6241 agaaaacacc ttttgtcgcg catccagcgc tacattcctg aggcgtattc acatcctttt
     6301 gattggtgat aacatgcgaa tcggtattat ttttccggtt gtaatcttca ttacagcggt
     6361 cgtattttta gcatggtttt ttattggctg ctatgctgcc ccgggagcat aaagatgaaa
     6421 aaaacaacga ttattatgat gggtgtggcg attattgtcg tactcggcac tgagctggga
     6481 tggtggtaac gtcacctcta aaaaatagca aaggctgcct gtgtgcagcc tttgtgcaat
     6541 ttaagcgtta acttttaatc ttcctgtaga taaatagcac gacaatcgca ccaataacgg
     6601 ctgaagcggc gcacgaaaaa cgctttcgcg tttttcgtgc gccgcttcac ccctggttca
     6661 gcatttcgtc tgcagtctga accgcaggca accatttttc agcaagtctg cgctgggaat
     6721 cgctggcttt atcccatttg ctccacagtg cctcacgatc gtaatcgtgc gcttccagcg
     6781 tggggcgggc gatttcaaaa tacccctagc ttgtttctat ttgctcagat tttaataaca
     6841 actcagcacg tgctgccgct ggtagaactg atatatgaaa ttccaaagcc tttgaacccg
     6901 tacgcttgcg acgtagttct ggatgattgg ctgcaagctt ttcaagattg cggcgaatac
     6961 tcacctcatg tttaggaaag catgacattc ctacgcattc ttgcgctgtt acccaatggg
     7021 ttaacatgat gttttctcca tataccgaga aggccaaatc tgggctggtt ccatatccaa
     7081 atatctagca atgattgctt cgtatcgtgg tatatggcag cgaaatacgt tacgcatagt
     7141 gtcaggcttt aagcctgcct tgaggtgtac tggcaatagc ggatactacc atttgttctt
     7201 tttttaagca gttatctgat gatatttttc cctgaaggct gccggggaga tattccccag
     7261 acgagagt
//
LOCUS       DAETQL010000156         7259 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.158, whole genome shotgun sequence.
ACCESSION   DAETQL010000156 DAETQL010000000
VERSION     DAETQL010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.158"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            29..880
                     /gene="hchA"
                     /locus_tag="K0W65_003900"
     CDS             29..880
                     /gene="hchA"
                     /locus_tag="K0W65_003900"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HBI3096967.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(988..2346)
                     /gene="hprS"
                     /locus_tag="K0W65_003901"
     CDS             complement(988..2346)
                     /gene="hprS"
                     /locus_tag="K0W65_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HBI3096968.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2346..3017)
                     /gene="hprR"
                     /locus_tag="K0W65_003902"
     CDS             complement(2346..3017)
                     /gene="hprR"
                     /locus_tag="K0W65_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBI3096969.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3150..3563
                     /gene="hiuH"
                     /locus_tag="K0W65_003903"
     CDS             3150..3563
                     /gene="hiuH"
                     /locus_tag="K0W65_003903"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBI3096970.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3672..4676
                     /gene="msrP"
                     /locus_tag="K0W65_003904"
                     /pseudo
     CDS             3672..4676
                     /gene="msrP"
                     /locus_tag="K0W65_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4677..5312
                     /gene="msrQ"
                     /locus_tag="K0W65_003905"
     CDS             4677..5312
                     /gene="msrQ"
                     /locus_tag="K0W65_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HBI3096971.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5570..6220
                     /gene="zinT"
                     /locus_tag="K0W65_003906"
     CDS             5570..6220
                     /gene="zinT"
                     /locus_tag="K0W65_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBI3096972.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         2057 a         1648 c         1519 g         2035 t
ORIGIN      
        1 ctaactaagc aacaataagg aatacactat gactgttcaa acaagtaaaa acccgcaggt
       61 cgatattgct gaagataatg cgttcttccc ttcagaatat tcgcttagcc aatatacgag
      121 tcccgtctct gatcttgatg gcgtggacta tccaaaaccg tatcgcggta aacataaaat
      181 tctggtgatc gccgcggacg aacgttattt gccgaccgat aacggaaaac tgttctcgac
      241 cggtaaccat ccgattgaaa cgttgctgcc gttgtatcat ctccatgctg caggtttcga
      301 attcgaagtg gcgaccattt ccggtctgat gaccaagttt gaatactggg ctatgccgca
      361 caaagatgaa aaagtaatgc cattctttga acagcataaa tcgttattcc gtaatccgaa
      421 gaaactcgcg gatgttgtcg ccagcctcaa cgctgatagc gaatatgcag caatctttgt
      481 tcctggtggg catggcgcac ttattggttt accagaaagc caggacgtgg ctgccgcttt
      541 acagtgggca atcaaaaatg accgttttgt tatctccctt tgccacggtc cggcggcttt
      601 tctggcactt cgccacagcg ataacccact gaatggttat tctatttgcg cattcccaga
      661 cgccgcagac aaacaaacgc cagatattgg ctatatgccg ggtcatctca cctggtactt
      721 cggcgaagag ttgaagaaaa tgggcatgaa catcattaat gacgacatca ccgggcgagt
      781 acataaggac cgtaaacgtc tcaccggcga cagtcctttt gcagcgaatg cgttgggtaa
      841 actggcggcg caggaaatgc tggcagctta cgcgagttaa tccctgttac attgtgatgt
      901 aagcgcataa tcaatcgcag ccacgttaat actcacgacg tggctgcgtt ttttcacggt
      961 taacttatgg cgtactgaag cccgatgtta atttctttgc ggtaacgtaa ttcggaacac
     1021 gttatgctta ctgagatagt gatacgtagc acttcctcca tgtaattcgg caatcgcttt
     1081 gactaaagaa aggccaagtc cctgacctac ggaatgacgc gaattatctc cccgccaaaa
     1141 tctacggaag agtttttcag gctcattaat tttcgttcca ggactggcga tatcaatatt
     1201 aagagagccg ttggcatcaa gaaaactggt tatatgaata cgcgattttt ctggtgaata
     1261 tcgaatggcg ttaacaataa gattcgataa cattcgttgc agtaaaattt tatccgcaaa
     1321 gatttgttga ttgcacttga ccttaaagca aatctctttc tcgtctgaaa gatattcaag
     1381 atagtccaaa agattttcaa cttccttatt gagagaaagc gaatccagtt tcaccaaaac
     1441 attgtttttg tctgccctgg caagaaacag tatattctct gttaaccgcg aaatattttc
     1501 cagctcttca atgtttccgg caattgtttt ttgatattca gcgatacttc tgatttgact
     1561 gagcgtaacc tgattctgac ccagtaatgc attaattggc gttctaagtt catgagcgag
     1621 atcgtcagca aattgactaa ggcgctcaaa atctttgact aaagcatgat gcattttatt
     1681 caacgcctgc ccaagaggtt ttagttctct cggtaatgcg ttaacctcaa ccggcactcg
     1741 gctatcgtta taattcagcg cttccgttac gccactcaac tttttgatct ctcgcaaccc
     1801 cgtcctgatt aacaacggac ttaatactga gcacagtata atggcgacaa tgcaaattat
     1861 aatgctatta attttatact gttcaagcat attatgtctg gctgaagcca atttagccac
     1921 agtaaccgtt aatgatggcg aaacttcatc aatgttaatt cgtaaagcat ctatctctgt
     1981 atcattaata atgcttctgt aaatgcctgc tgcgctgatt gtctcactag caggtatgtt
     2041 atttaacatg tcatcactga catttgtccg gttaacaatt ttattgatgc catcactatg
     2101 aatgatcaaa atatcctgac taacatccat catccggtta aagtacacag gtaacgtatc
     2161 tggatttacc ccatcaatta acaactgctt gatctgcgct gtccggttaa tgagtgttgt
     2221 atcatcgcgc catttcaact cacttgccag gccattatag agagtccata caattccagc
     2281 gccagcaaca gactgcagaa atataaaaag caaggttaaa cggacggtta tagataatct
     2341 tttcattatt tttttaccgc tacgaatgaa tagcccatcc cccggattgt ggcgattagc
     2401 ttttcaggaa aaggatcatc aacttttgcg cggagcctgc gaatggcgac atccaccgta
     2461 ttggtatcac tatcaaagtt gattccccaa atttcactcg caataaccgt tctgggtata
     2521 atctcgccag ctctggaggc cagtagccaa agtaactgaa actccttgcg cgtcagtgta
     2581 atactgatat tgtccctgct gacactttga ctgacagagt ccatttttaa gccactgatt
     2641 tctaatgttg aattcagagc gtgatgttgc cttaattgtg cccgaaccct tgctagcaac
     2701 tcagaaaatg aaaaaggttt taccagataa tcatttgccc cactgtccaa ccctctgact
     2761 ctgtcatcga cagaatccct tgcagtaagg caaataacag gggtttgctt tgccgttctt
     2821 aacgtttgta agatctgcca gccatccata cccggaagca taatatccag aatgatcaat
     2881 gcataatcat ccttcagcgc aagataaagc ccatctctgc catcagaaac ggcatcaatg
     2941 acataacccg cttcggaaag cccctgcgtt acccattcct gggtcctttg attatcttca
     3001 ataagtagaa tcttcatgaa aataatatgc cataaatcat atgttataga aacaacctgg
     3061 ttcattacaa aattgtaatg ctgctgtaag gttaccctgg acgttttttc gctatcctca
     3121 aaactcattc acatgacaag gatataaaca tgttaaagcg ttatttagta ctctcggtag
     3181 caacggcagc cttttcatta ccttctttgg tttatgccgc acaacaaaac attcttagcg
     3241 tgcacatttt gaaccagcaa acaggaaaac ccgctgccga cgtgacagtc actcttgaaa
     3301 agaaagcgga taacggctgg ttacaactta ataccgccaa aacagataag gatggacgaa
     3361 ttaaggcact gtggcccgag caaactgcga ctactggcga ttaccgtgtc gtatttaaaa
     3421 ccggggacta tttcaagaaa caaaatcttg aaagtttctt ccctgagatt cccgttgaat
     3481 ttcatattaa taaagtgaac gagcattatc atgtgccttt gttactcagc caatatgggt
     3541 attcaaccta tcgtggcagt taatgcgtaa atatgtaaaa gctgatctct gctactgtta
     3601 gggaggtgac tccctgacag attaacagta aacagctctt gttggttaac gacaaaaaag
     3661 tgtgatggct tatgaaaaag aagcaatttt taaaagaatc agatgttacg gccgagtcgg
     3721 tattctttat gaagcgtcgg caggtgttaa aagcactggg catcagcgca gctgcatttt
     3781 ctttgcctca cgctgcgcat gccgatctgc ttagctggtt taaagggaac gatcgtccgc
     3841 ccgcccccgc cggaaaaccg ctggagttca gcaagcctgc cgcctagcaa aataacctgc
     3901 cactgacgcc agcagataaa gtctccggtt ataacaactt ctatgaattc gggctggata
     3961 aagacgatcc cgcggctaat gctggtagcc tgaaaaccga tccatggaca ctgaaaatca
     4021 gcggcgaagt ggcaaaacca ttgaccctcg atcatgatga tttaacccgt cgcttcccgc
     4081 tggaagagcg tatttatcgt atgcgctgcg tggaagcgtg gtcgatggtg gtgccgtgga
     4141 ttggttttcc gctacacaaa ttgctggcgc ttgccgaacc caccagcaat gcgaagtatg
     4201 tcgctttcga aacgatttat gcgccggaac agatgcctgg ccagcaggac cgctttatcg
     4261 gcggcgggct gaaatatcct tatgtcgaag gattgcgtct cgacgaagca atgcatccgc
     4321 tcacactgat gaccgtaggt gtttatggca aggcgttacc accgcaaaat ggcgcgccgg
     4381 tgcgactgat tgtgccgtgg aaatatggct ttaaagggat taaatcgatc gtcagtatta
     4441 agctgacccg cgagcgtccg ccaaccacct ggaatctggc agcgcctgac gaatacggtt
     4501 tttacgccaa cgttaatccg catgttgatc acccgcgctg gtcacaggct accgaacgat
     4561 ttattggttc aggcggcatt ctcgatgttc agcgccagcc aacactactg tttaatggtt
     4621 acgccgacca ggtggcatcg ctgtatcgtg gcctggattt gcgggagaat ttctaaatgc
     4681 gtctgacagc aaaacaagtg atatggctga aagtttgcct gcatcttgcc ggattgttgc
     4741 cgtttctctg gctggtctgg gcgatcaatc acggtggact gggtgccgat ccggtgaaag
     4801 atattcagca ttttaccggt cgcactgcac tgaaattttt gcttgcggcc ttgttaatca
     4861 cacctctggc acgctacgca aaacagccgt tattgatacg cactcgccgc ctgttaggct
     4921 tatggtgctt tgcctgggcg acactgcatt taaccagtta cgcattgctg gagttgggcg
     4981 tgaataatct ggcgttatta ggtaaggagt taattacccg gccttattta acgttaggaa
     5041 ttatcagctg ggttattttg cttgctttag cgttcacttc aacccagtcg atgcagcgaa
     5101 aactcggcaa acactggcaa cagttgcata acttcgtcta tcttgtcgcg atcctggccc
     5161 caatacatta tctgtggtca gtgaagatta tttcaccgca gccactcatc tacgccgggc
     5221 tggctgtact gcttttagcc ttacggtata agaagttgct ttcccttttc aaccggttac
     5281 gcaagcaggc ccacaacaaa ttatctctat aacttcatat tgccgacaaa cttcgcgtta
     5341 tcccggccgg atgcggcgcg agcgccctat ccggcccaca cagatcgcat caagttatgt
     5401 ttcgtttgtc atcaaccttt catcaccaga ataatttatt ttatttcaat aagatagttc
     5461 aatacacctg agaataccat gctctcattt cctaatgagt tgttgcattt tttcatatgt
     5521 tacaatataa cattacacat aatgtacatt aactttggag gaaattgttt tggcgattcg
     5581 tcttcataaa ctggctgttg ctttaggtgt ctttattgtt agcgctcctg ccttttcgca
     5641 tggtcatcat tctcatggca aacccttaac agaggtcgaa caaaaagctg ctaatggtgt
     5701 ttttgatgat accaatgtac aaaaccgaac gctcagtgac tgggatggag tctggcaatc
     5761 cgtttaccct ttactgcaaa gtggcaaact tgatcccgtc tttcagaaga aagcggatgc
     5821 agataaaacc aaaacatttg ctgaaattaa agattattat cgcaaaggtt atccgacaga
     5881 tatcgaaatg attggcattg aggacggcat tgtcgaattc catagaaata atgaaacaac
     5941 atcctgtaaa tatgattacg atggatacaa aatactcacc tataaatcag gcaagaaagg
     6001 cgttcgttac ttattcgaat gtaaagatcc tgaaagcaaa gcccctaaat atatacaatt
     6061 tagcgatcat ataattgcac cacgaaaatc atctcatttt cacattttta tgggtaatga
     6121 ttcccagcaa tctttattga atgaaatgga aaactggcca acgtattatc cctatcagtt
     6181 gagtagcgaa gaagtggtcg aggagatgat gtctcattga tttttttgcg ctgacttcag
     6241 ttatttaaca gaacagtgcc tggtagtaac aacaataatt taccaagaca aataagtaag
     6301 acaaactcag acacgctcat acttgagtcg tgtctttttt attatgtctg tagaaaaaaa
     6361 ctgagaaaaa ggctaacaat aaatacacaa ctcagccata cagactggca gcgcaaatgt
     6421 ttccaggagc aagtcatctg tcatggtaaa gctaaccaga aatataggtt aattttggtg
     6481 tttaaacctt ttttacttgt tagtatgata caaaggcttt caaaaaagct gggaaattca
     6541 acaactcttg gaaattatgg actttgtccc gcggctttaa tctgacggcc gcgttccttt
     6601 tttggctcaa ttattgaccc cttctctacg caacttcagt tcccgccagc aactttgcgg
     6661 cagtttcata ggatcaatgt ctaaaagaat aatggtgacc gataagaaaa cgactgaata
     6721 actgtagatt ttcgctcgaa accttcctgt cagctccata gcgaatcaag cgctgaatgt
     6781 cacagtatcg aacagaaaac agtgacgatc taacccttca agaatattct acgattgttc
     6841 tgtttaggaa aagcaaggcg ggaagtcggg agataagtca ttgataaaat ggcggagaga
     6901 gggggatttg aacccccggt agagttgccc ctactccggt tttcgagacc gatgatacag
     6961 agtcgaaaaa tcaaagagtt aaaaattttt cttggaaaaa atgtcttaaa atcgtacacg
     7021 aaaaatcaat gaattacata acattaaaga agaatattcc aagccttctt gagtccacca
     7081 cctgttttca ggtgtcattc agacgtgatt tgccccagat tgggaaagca cgcttttttc
     7141 tcagcttatt ccgttttgcc tgtgtgttga ataattccac tgcagttata taaaatcggt
     7201 aacggctgga aatcattcaa tactcgcact atcgaaagtt taccagccaa ccgcagcac
//
LOCUS       DAETQL010000157         7183 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.159, whole genome shotgun sequence.
ACCESSION   DAETQL010000157 DAETQL010000000
VERSION     DAETQL010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.159"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(296..1198)
                     /locus_tag="K0W65_003907"
     CDS             complement(296..1198)
                     /locus_tag="K0W65_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBI3096973.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="K0W65_003908"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="K0W65_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HBI3096974.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="K0W65_003909"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="K0W65_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HBI3096975.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="K0W65_003910"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="K0W65_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HBI3096976.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="K0W65_003911"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="K0W65_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HBI3096977.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="K0W65_003912"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="K0W65_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="K0W65_003913"
     CDS             7090..>7183
                     /locus_tag="K0W65_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HBI3096978.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1676 a         1812 c         1876 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAETQL010000158         6929 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.161, whole genome shotgun sequence.
ACCESSION   DAETQL010000158 DAETQL010000000
VERSION     DAETQL010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.161"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            240..2672
                     /locus_tag="K0W65_003914"
     CDS             240..2672
                     /locus_tag="K0W65_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBI3096979.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="K0W65_003915"
     CDS             2739..4208
                     /locus_tag="K0W65_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBI3096980.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="K0W65_003916"
     CDS             4208..5869
                     /locus_tag="K0W65_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HBI3096981.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..6431
                     /gene="symE"
                     /locus_tag="K0W65_003917"
     CDS             6090..6431
                     /gene="symE"
                     /locus_tag="K0W65_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HBI3096982.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6478..6798)
                     /locus_tag="K0W65_003918"
                     /pseudo
     CDS             complement(6478..6798)
                     /locus_tag="K0W65_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2050 a         1640 c         1585 g         1654 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaagctggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
     6361 tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga
     6421 aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta
     6481 taccaacgcg ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg
     6541 aagttgtgcg aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt
     6601 tgtcacatct ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga
     6661 caatcgcagt cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc
     6721 aatatatagc ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg
     6781 aagatgatcg aataacactg aggtagcctg agtttaacgg acactccttc ctgaaataga
     6841 atggcatcag aaggagctaa taatgagcag aaaaacccaa cgttactcta aagagttcaa
     6901 agccgaagct gtcagaacgg ttcttgaaa
//
LOCUS       DAETQL010000159         6910 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.162, whole genome shotgun sequence.
ACCESSION   DAETQL010000159 DAETQL010000000
VERSION     DAETQL010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.162"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..694
                     /locus_tag="K0W65_003919"
     CDS             <1..694
                     /locus_tag="K0W65_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBI3096983.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1033..1803)
                     /locus_tag="K0W65_003920"
     CDS             complement(1033..1803)
                     /locus_tag="K0W65_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096984.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1848..2282)
                     /locus_tag="K0W65_003921"
                     /pseudo
     CDS             complement(1848..2282)
                     /locus_tag="K0W65_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2294..2515)
                     /locus_tag="K0W65_003922"
     CDS             complement(2294..2515)
                     /locus_tag="K0W65_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096985.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2516..3199)
                     /locus_tag="K0W65_003923"
     CDS             complement(2516..3199)
                     /locus_tag="K0W65_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBI3096986.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3263..3574)
                     /locus_tag="K0W65_003924"
     CDS             complement(3263..3574)
                     /locus_tag="K0W65_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3096987.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3571..4473)
                     /locus_tag="K0W65_003925"
     CDS             complement(3571..4473)
                     /locus_tag="K0W65_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBI3096988.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4913..5872
                     /locus_tag="K0W65_003926"
     CDS             4913..5872
                     /locus_tag="K0W65_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBI3096989.1"
                     /translation="MLKVSCDDGSTNVKLAWFEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5872..6267
                     /locus_tag="K0W65_003927"
     CDS             5872..6267
                     /locus_tag="K0W65_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBI3096990.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6450..6830
                     /locus_tag="K0W65_003928"
     CDS             6450..6830
                     /locus_tag="K0W65_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HBI3096991.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1888 a         1785 c         1675 g         1562 t
ORIGIN      
        1 tgatgcagaa gccatatgtg aagcggcttc acgaccatca atgcgttttg tccagccaag
       61 aactgagacc cagcaagcaa taagggcatt acaccgggtc agagaatcgc tcatcagaga
      121 taaggtgaaa acaaccaacc agatccacgg tttcctgctg gaatttggaa tcagcctgcc
      181 gacaggggat gccgtaataa aacggttgtc tctcgtattg gctgaacacg aaatccccga
      241 atatctgagc cgccttttgg tgaggttgca tacccactac ctttaccttg ttgagcaaat
      301 agcagagctg gaatcagaat taagtcagtc aatcaacgct gatgataccg ctcagcgcat
      361 tatgacaatt cctggtgttg ggccgattac tgccagctta ctatcgtcgc aacttggtga
      421 cgggaaacag ttttcatgca gcagagattt tgcggcttcc acagggctcg ttccccggca
      481 gtatagcact ggaggaaaat cgactctgtt gggtatcagt aagcgaggtg ataaaaattt
      541 acgccggtta ctggttcagt gtgcccgctc attcatgatg cagcttgaac gccagcatgg
      601 aaaacttgct gagtgggttc gggagcaact caacaagaag cattcgaatg tggttgcctg
      661 cgctctggca aataaactgg cccgaattgc ctaggcaatc actactcagc aaaaagaata
      721 tcaggcctga tatatagatg ctgacaaaac tcaattagct tattgcaaac aataagatac
      781 aagttaccgt ctggttttgc gaagattgat tattgatgac atgaacggca aatcgacctg
      841 attaaaaacc taacagggga aaatggctca ctgaagccga ggggattttt aggattatca
      901 ggtgcggaac tcatcgaggc gcgggcatcc cttgcccaca gagacgccgg atagatttaa
      961 gcaagcccac cattaatcaa aaatcagtgt tgcaaaaacg ggggtgacca tagattttcc
     1021 ccgccatcgt catcatttcc ggatttttgc cagggcatcc gccatgctgc ggatttcggc
     1081 ccgggcttcc cggacaactt cccgcagaac accacggtca aaccaccggc ggggcggttc
     1141 atccggtata cgcagcacat cgcaggccag cccactggac aggatatgct caatacaatc
     1201 gctgaccaga tacatttcca ccgccgaaaa acgtgcgccc cggttcagga tggcatcgag
     1261 caccacgggc aggttttcat cgtcttcatc gagccagcag gggtgatcgc tttccttcac
     1321 gaccaggcga aagcggcgcg tctggcgggt cagcaacgtc tgaccgcttg tggtcagcgc
     1381 cgcccggata cgccgcgcat cttctgcccc ccggcaactg acactgcata ctgcatcgcg
     1441 caccgcatca agaactgccc tgatttcgtc ttcggtcagc cattcaccgg catacgggaa
     1501 agacacctgc gttccgtccg ccggaagtac cggcagcgac tgccactgac tgccggacag
     1561 aacaagttcc gctccggttc cggtcagttt ctgaatggcg gaggccaggt gcggcggcag
     1621 cacgccatcg tggcggttaa cgagtaacgt cgccagccgg ggcgcatcca gccgcacgta
     1681 aatgctgatc cagcccggat agttcgggtt acattggtca acatccgtct cttccggcca
     1741 gccctgcgcc agcagcaggt tacgggcgtg acgacaaatc agagcattca gcgtttcatt
     1801 cataattcat gaactccctg tatcgccgcc cacaggcggc gataacatca gtcgttttcg
     1861 tgtgtgttgt ttttcagcag gctgcgggcc tgatcaacca tcgcctgtcg gcgggtaacg
     1921 tattccggga gggggaaacc gtgatcccgg gcagcccatt cacgtttcca cagtgcctgt
     1981 tcagcgatct gagtcagggt ctccagcagt tcagcaggga cggtcacctg gcggttttcg
     2041 cgcacgttgc tgataatctc catcgccgcg gcggaagaaa taccggactc aatccgctag
     2101 tcttccggga tgtcctccag gtgcgccagg acggtcctga tttcatgatc ggacagggaa
     2161 atatccattc cgtcagcgag ggcctgaatc tcttccgggg accagataac cagcatcagc
     2221 ggcgtgtctc ccgaatactt tgctgccagc gcccggcata gcgtttcgca tgttccgtac
     2281 atactctctc ccctcaatct ttgaagcagg aatccagttc cagaatttcg gtatatgcct
     2341 ccatcaagag gcggtttgcg cgaaacaacg tctgacgcac cagacgttcc ggcagggtgt
     2401 cagcattaaa gctgtaccgc cgctccatct cattcatggt tgtacgcagg tcgcacatgg
     2461 cattggccac ctccgccact tcggcgcgaa gtttttcgtg tccggcatag ttcatttacg
     2521 cagcctccgg cataaaccag tcatcattcg cggccccctg ctgcatggcc cgctgtacgg
     2581 cagccagtcg ttgctgtccg gcacggtgat actgctcaag cagctcgata ccgatatacc
     2641 ggcgtccgga ctggagggcg gcgacgcagg ttgaaccgct gcctgcaaac gggtccagca
     2701 ctatcgcatt cgggtgtgtg aagctctcaa tcagcggttg caggctggta acgggctttt
     2761 ccgtcgggtg atggcggttg cccgaatatt tccagcccaa cacgtccggc agcgggtttt
     2821 gtggcagacg tggacggcct tttgccagga tgtaggcgca ttcgtggcga tagcccacat
     2881 atgcggcctt cgatgtgtag tttttggtga agaccaggtg accaacaacg ctgaatcccg
     2941 catttttcca ggcagacata aagcgatcga cgcggttcca gccatagaag ctcaccatca
     3001 gcgcgtcttt tttgagtacg cggtacattt cattacaggc cggttgcagc cattcatcag
     3061 ttttatcacc agcaatggta cgcccctgac ggtcacgaaa accgacgaga tatggcggat
     3121 cggtgaggat gaaatcaatg gcgttatcag gaatacgggc cataacatcg atgcagttac
     3181 cgaggacaaa acgggacata acagatcctt ctcttacaga gggcacccct gtggtgcccg
     3241 ttccccgctg cctgtgggct gttcatcgct tccggtaacg ggtcaggccg accgtggaat
     3301 accgcagccg tcgtcttttt gacagcaagg ttatgccgcg aaagcggctt gaagggcgtt
     3361 catcagaaaa gggcgacggg ctggcgtaca ctgtcaccga caccagccgg gagcggtaaa
     3421 agcgcccgtc ccgtgcccgc cggacgcggg acactgacca caggcagcgt ttatgccgcg
     3481 ccgcgtcagc cggcgcagta aaaagaggag cacggcgaca tcctttgtcg gaaaagaaac
     3541 gggcggttgc ccgcccgtct ccgtcatact tcatccgcca taatgcgcca cattgtcgac
     3601 tatccacgca ggtccggtga cttccggctg ctcatcgtca tctgtcgggg aagaccattc
     3661 aagttccccc cacagcacat tgacgagctc tccacgctca tacaactgcc agccacagaa
     3721 atcacagccc tgttcggcat accagtgctc cagcgtgcag ctgaaacggc ggcttaattc
     3781 tgcaataacg tcgctttccg gctgacacca cggagtgtcg aaatccacct gaatgaagtt
     3841 ttctccctga ctgctgatat tcaggtcata tccacaaggc cacttcacgc cataccgttc
     3901 cgtataccag tgatatgcag aaggaacacc gttcagaaca ccaccattaa agccgttcac
     3961 ttcaacatcc aggcgggtcg gcagaaccgt cagcaggtca aacggcatgg ttttttccgg
     4021 cactacgttg tcacacagac ggttccacca caccgataca tcctcgttac cccatatgtc
     4081 acaccagtca cctctttttg cggtgaaatg cacagtgatg gtttccctgg catcatccgg
     4141 taatccttcc caacgacgct ggccagtacc gctctgtaac cagagttcat gcagcatcag
     4201 gcagttttgt tcatccagca acacaccgtt ctgcaggtgt gtcagccagc gggtgaacgc
     4261 gatattttcc gtcgatacag caccacgtcc ggcagccgtc actccggggc actgttcaga
     4321 gcggatattt tcagtgatct gcagaagtcc ggcacttccg gccagaaaca gctgaatacc
     4381 ttcatttgtg gcgcggcgat aaagcggtgt gactgcaccg ctggccagtc gtttaatctc
     4441 agcgatctgt gccggctcac caggaaaata catacgattc gagcaccagt ttggcatggg
     4501 aattctcctc tcacatgcgg cctccatcag gccgtgccgc acctgtaacc gggcgaccgc
     4561 atacgaatgc aggtgcggcc ccgaagccgc agcgcgtgga ggaggaatga cgaaacgtga
     4621 ccgggtgagg aggccggcga cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc
     4681 cggcacggcc tggttaaata gtatccgtct cagttcgtgg cgcaaaatga gaaaccaata
     4741 agaactcaaa atgaaccaca acaagaacat aaagagactc taaaagagca acaccgtttg
     4801 acatagactc aaaatgagtc acaataagaa acaataaaaa actcaaaaag atgcaccgac
     4861 ataaaggatg ctcaatttga gataaacaca aactcaaaac gaggcaaaaa gaatgctaaa
     4921 agtatcatgc gatgatggtt caactaacgt aaaactggcc tggtttgaag atggcgaggt
     4981 aagaaccagt ctgtcaggaa acagctttaa agaaggctgg aacccgggac tgtttaatgc
     5041 cggcaaagtt tacaattacg tcgtggatga aaaaaaatac acctatgatt tgggaagcac
     5101 tgctgtcata ggtacaacac atgtcagtta tcaatacagc accaccaatc tgcttgccat
     5161 acatcatgca ctgctgacat ccggccttca gccacaggat gtagaactga cagtaacatt
     5221 acctgtgacc gaattctttg ataatgacaa tcaaccaaat gaagaaagaa tagaaagaaa
     5281 aaaggcaaat gttctccgtg agatatctct gaataaaggg gaaacattta aaattaaaaa
     5341 agttaatgta atgcctgaat ctttgccagc tgcatttgaa tcactaaaaa aagataaagt
     5401 aaacaaactt gaacgcagcc tgattatcga tcttggagga accactctgg attgtggttt
     5461 aattctcggt gcatttgaag gcatttctga aatccgtggc tattcagaaa tcgggacatc
     5521 ccgcatcact cataccgtca tgaatgcgct gacgaaagcc tccacaccat gtaattattt
     5581 tattgctgat gagttaataa aaaacagaca cgataatgaa tatctccaga cattaataaa
     5641 tgatgtagct gaaataaaaa acatttctca tgtaatagac agagaagtga aatctctggc
     5701 agaaagcatc aggcaggaaa tttcaacatt ctcaggaatg aaccgaatct atctcactgg
     5761 cggaggagcc gagcttattt accctcacat aaagcaatat ttcccgaatc tgaaagtgaa
     5821 caaggtggat gaacctcagt ttgcattggt gaaagcaatg gtgcatgcgt aatggaaagc
     5881 agtgatccaa aaaaaagaaa gaaagttgtg gcatacctgc acccggcact ttatccgcag
     5941 gacaacctga cacaacaaac aatagactct ctcccagtac aaatgcgggg agatttctac
     6001 agacaatccc ttatttgcgg agcagctctt tattctgttg ccccccgact gttaacgcta
     6061 atcagcgttt ttttcagtga gaaaatcact gcagaaaatc tggtgaaact tatagaacaa
     6121 acaacaggat acacatccac atcaattgat ataagtgtgt taaaaaacat catagaggca
     6181 tcttcagaaa acaaatcaga gagcatcact tcaaaagatg atttcgaaga acaaaccaga
     6241 cgcaatcttt ctatgttaaa aaaataataa aagaagagca ggttcagtca attacctgct
     6301 cttcttttat acatactaaa aaatcagatt tttacgaata ttcagattat cataaaaaat
     6361 gcaacagaca ggaatgcaat aattttgcat tatctcgttg cttgtaatgc aaatatgcat
     6421 tattgtcagt gtgtgacaat aacagagaga tgacgatggc gaccattaac gcacgtattg
     6481 atgatgatat aaagaaccaa gcagatgaag tgctgaagct gatgaatatc agtcaaactc
     6541 aggctattgc agctttttat cagtatatta ctgaacaaaa gaaactgcca tttgtgatca
     6601 cctcaattgt aaaaacgccg catgatttat tacgtgaaag tacagacatg ttagctgaag
     6661 ctcttgcagt gatttccaat ctacaggtgt ggactgaaca gcaggatggg attggaaaag
     6721 caaagctaat ggagtattac cgccgcctcg acgcactgta ctgttgtgca aaagagaaaa
     6781 taggtcttct ttcagataac cgggatgctg aactaggctg tgtcccttaa tcatctgagt
     6841 tatagtaact gtctgtttac acaacatatt gaactatggc tcgctacgac cttcccgatg
     6901 aggcatggac
//
LOCUS       DAETQL010000160         6523 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.163, whole genome shotgun sequence.
ACCESSION   DAETQL010000160 DAETQL010000000
VERSION     DAETQL010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.163"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            442..1830
                     /gene="narU"
                     /locus_tag="K0W65_003929"
     CDS             442..1830
                     /gene="narU"
                     /locus_tag="K0W65_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HBI3096992.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1912..5652
                     /gene="narZ"
                     /locus_tag="K0W65_003930"
                     /pseudo
     CDS             1912..5652
                     /gene="narZ"
                     /locus_tag="K0W65_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5649..>6523
                     /gene="narH"
                     /locus_tag="K0W65_003931"
     CDS             5649..>6523
                     /gene="narH"
                     /locus_tag="K0W65_003931"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBI3096993.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1558 a         1714 c         1692 g         1559 t
ORIGIN      
        1 gaccttagat gaaataaaca atacttacta taataacgat tattacaatc aggcaataag
       61 agaggaaccg atagactttc tggacagatc gttttcctcc aattcatggc ctttttagtg
      121 aataaatcat cactaattct ataagtgtct ggtcttgttg agaatatagt taatatgtaa
      181 ccctttagat tcacgatctg aagggttgaa acatgacata cgttcaaaga ttaacccccg
      241 tttgaaggtt agcttgttag ttacatttag taacacatat ctggctactt aatccccatt
      301 ctgcccccgc ggttacgctt tcttatctat tctttcaatc agcacttcac ccaattcatc
      361 cttaattcta ataactggct attgcgcctt acctcctgtt ttgtcttttt tcattaccaa
      421 tgtgtgcata tgaggaacaa tatggcactg caaaatgaga aaaatagtcg ttatctttta
      481 cgcgactgga aaccagaaaa tccggccttc tgggaaaata aaggaaaaca tattgctcga
      541 agaaacctct ggatatcagt cagttgtcta cttcttgcct tctgtgtctg gatgctattt
      601 agcgcagtta ccgttaatct caataaaatc ggttttaatt ttactaccga tcaactcttt
      661 ttattaaccg cattaccctc cgtttctggc gcattattgc gtgttcccta ctcctttatg
      721 gtgcctatat tcggtggacg ccgatggccg gtttttagta ctgcaatcct gattattcct
      781 tgcgtctggc tcggaattgc cgtgcaaaat ccgaatactc cttttgggat atttatcgtt
      841 atcgctttgc tatgcggttt tgcaggtgca aactttgctt cgagcatggg caatatcagt
      901 ttcttctttc caaaagccaa acaagggagc gctcttggga ttaatggcgg attaggaaac
      961 ttaggtgtaa gtgtaatgca gctggttgca ccgctggtca tttttgtacc tgtatttgcc
     1021 tttctcggcg tcaatggcgt accgcaggcc gacggttcgg tgatgtcgct ggcgaatgcc
     1081 gcatggattt gggtaccgct actggcgatt gccacgatcg ccgcctggtc agggatgaat
     1141 gatatcgcca gttcacgcgc ctcaattgcc gaccagctcc ctgtcttaca acgcctgcat
     1201 ctctggctgc tgagcctgct ttaccttgcc accttcggtt cgtttatcgg tttttctgcg
     1261 ggttttgcca tgctggcaaa aacccagttc ccggatgtga atattctgcg cctggcgttc
     1321 tttggcccat ttattggtgc catcgcgcgg tcggttggtg gtgctatttc cgataaattc
     1381 ggcggcgtgc gggtgacgtt gatcaacttt atttttatgg cgattttcag tgccctgctg
     1441 ttccttacct taccgggcac aggctccggt aatttcatcg ccttttacgc cgtatttatg
     1501 gggctgtttc tgaccgcggg tctgggaagt ggttctactt tccagatgat cgccgtcatc
     1561 tttcgccaga taaccattta tcgggtgaag atgaaaggcg gtagtgatga gcaagctcag
     1621 agagaagccg tcaccgaaac ggcggcaacg ctgggcttta tctcagccat tggcgcagtg
     1681 ggcggctttt ttattccgca ggcatttggc atgtcgctca atatgaccgg ctctccggtc
     1741 ggcgcgatga aagtgttttt aatcttctac atcgtttgtg tgctgctgac ctggctggtt
     1801 tatggtcggc ggaagttcag ccaaaaataa gagatcgatg cctgatgtac aaccatgtcg
     1861 catccgacaa ggcatcagat cccaattcat tcctggagca ggagtcatgt catgagtaaa
     1921 cttttggatc gctttcgcta cttcaaacaa aagggcgaaa cctttgccga tggtcacgga
     1981 caggtgatgc atagcaaccg cgactgggag gacagctatc gccagcgttg gcagttcgac
     2041 aaaatcgtgc gttccaccca cggcgttaac tgtacaggct cctgtagctg gaaaatctac
     2101 gttaaaaatg gtctggtgac ctgggaaatc caacagaccg actacccgcg cactcgccct
     2161 gacctgccca atcatgaacc tcgcggctgc ccgcgtggcg caagttactc ctggtatctt
     2221 tacagcgcta accgcctgaa atacccgctc attcgtaaac gactgattga actgtggcgc
     2281 gaagccctca aacaacacag cgatccggta ctggcgtggg catcgattat gaatgatccg
     2341 caaaagtgcc tgagctacaa acaagtgcgt gggcgcggcg ggtttatccg ctccaactgg
     2401 caggaactaa accagctgat tgccgccgct aacgtctgga ccatcaaaac ctacggcccg
     2461 gatcgcgttg ccggtttctc gccgatcccg gcgatgtcga tggtttctta cgccgccgga
     2521 acgcgttatc tgtcgctgct tggcggcacc tgttaaagtt tctacgactg gtattgcgac
     2581 ctgccgcccg cctcgccgat gacctggggc gagcaaaccg acgtaccgga atctgccgac
     2641 tggtataact ccagctacat catcgcttgg gggtctaacg taccgcagac acgtacgccg
     2701 gacgcccact tctttaccga agtacgctac aaaggcacta aaaccatcgc cattacccct
     2761 gactactctg aagtggccaa attgtgcgac cagtggctgg caccgaaaca aggcactgat
     2821 agcgccctgg cgatggcaat ggggcatgtg attttaaaag agtttcatct cgataatccc
     2881 agcgactact ttatcaacta ctgccgccgc tacagcgaca tgccgatgct ggtaatgctg
     2941 gagcctcgcg acgatggtag ctacgttccc gggcgcatga tccgcgcatc tgacctggtg
     3001 gatggactgg gcgaaagcaa caatccgcag tggaaaaccg tagcagttaa taccgcaggt
     3061 gaattggtag tgccgaacgg ttcgattggt ttccgctggg gagaaaaagg caaatggaat
     3121 ctggaatcca ttgccgccgg tacggaaacc gaattgtcgt taaccctgct cggtcaacat
     3181 gacgctgttg caggcgtggc cttcccctac tttggcggca ttgaaaatcc gcattttcgc
     3241 agcgtaaaac acaatccggt gctggtgcgc caattgcccg ttaaaaacct gacgttagtc
     3301 gatggcaaca cctgtccggt ggtcagcgtt tatgatttgg tactggcgaa ttacggcctc
     3361 gatcgcgggc tggaagatga aaacagcgcg aaagattacg ctgaaatcaa accgtacacc
     3421 ccagcctggg gtgagcaaat taccggcgta ccgcgccagt atattgaaac catcgcccgc
     3481 gaatttgccg ataccgccca taaaacgcat gggcgctcaa tgattatcct cggcgcaggt
     3541 gttaaccact ggtatcacat ggacatgaac taccgcggga tgatcaatat gctgatcttc
     3601 tgcggttgtg tcgggcaaag cggtggcggc tgggcacact atgtcggcca ggaaaaactg
     3661 cgcccacaaa ccggctggtt gccactggcc tttgcgctcg actggaaccg accaccgcgc
     3721 cagatgaaca gcacctcgtt tttctacaat cattccagcc aatggcgcta tgaaaaagtc
     3781 tctgctcagg agttactttc accgctcgcc gatgccagta agtacagcgg tcatctgatt
     3841 gatttcaacg ttcgcgccga acgtatgggc tggctacctt ctgcgccgca gctggggcgt
     3901 aacccgctcg ggattaaagc tgaagccgac aaggccggat tatcccccac agaatttacc
     3961 gcccaggcgc tgaaatcggg cgatttacgt atggcctgcg aacaaccaga tagcggcagc
     4021 aatcatccgc gtaatttgtt tgtctggcgc tctaacctgc ttggctcctc cggcaaaggc
     4081 cacgagtata tacagaagta tctgctgggg accgaaagcg ggattcaggg cgaggaactc
     4141 ggtgccagcg acgggatcaa accggaagaa gtcgagtggc aaactgcggc gattgaaggc
     4201 aagctcgacc tgctggtgac gctcgacttc cgcatgtcca gtacctgcct gttctccgat
     4261 atcgttctgc ccaccgccac ctggtatgaa aaagacgata tgaacacctc ggatatgcat
     4321 ccatttattc atccgctttc tgcggcggtc gatccggcct gggaatcacg cagcgactgg
     4381 gaaatctaca aaggtattgc caaagcattt tcgcaagtgt gcgtgggcca tcttggcaaa
     4441 gaaaccgacg tggtattaca accactgctg catgactctc cggcagagct ctcacagccg
     4501 tgtgaagtgc tcgactggcg caaaggcgaa tgcgatctga tcccgggtaa aaccgcgccg
     4561 aatattgtgg cggtggagcg cgactaccct gctacgtatg aacgctttac ctcgctcggg
     4621 ccattgatgg acaaacttgg caatggcggt aaagggattt cgtggaatac ccaggatgaa
     4681 atcgatttcc tcggcaaact caattacacc aagcgtgatg gcccggcaca ggggcgtccg
     4741 ctgattgaca ccgccattga cgcttcagaa gtgattctgg cactggcacc ggaaaccaac
     4801 ggtcatgttg cagtcaaagc gtggcaggcg ctgggcgaga tcaccgggcg cgaacatacc
     4861 catctggcgc tgcacaaaga ggacgagaag attcgctttc gcgatattca ggcgcagccg
     4921 cgtaaaatta tctccagccc cacatggtcc ggtctggaaa gcgatcacgt atcctataac
     4981 gcgggataca ccaacgttca tgagttaatt ccgtggcgca cgctgtcggg acgccagcag
     5041 ctctatcagg atcatccgtg gatgcgtgct tttggtgaaa gcctggtggc ttatcgcccg
     5101 cctatcgaca cccgtagcgt cagtgagatg cgccagatac cgccaaacgg cttccaggaa
     5161 aaagcactta acttcctgac gccgcaccag aaatggggca ttcactcaac ctacagtgaa
     5221 aacctgctaa tgctgacgct ctctcgcggt ggaccgattg tctggatcag cgaaacagat
     5281 gcccgagaac tgaccattgt cgataacgac tgggtggaag tatttaacgc caacggcgcg
     5341 ctgactgccc gcgcggtggt cagccaacgt gtaccgccgg gcatgaccat gatgtatcac
     5401 gcccaggaac gcattatgaa tattcctggt tcggaagtaa ctggcatgcg cggcggcatt
     5461 cataactcgg tcacccgcgt ttgcccgaaa ccgacgcata tgattggcgg ttacgcgcag
     5521 ctggcctggg gctttaacta ctacggcacc gtcggttcga accgcgacga attcatcatg
     5581 atccgcaaga tgaagaacgt taactggctg gatgatgaag gtcgcgatca ggtacaggag
     5641 gcgaaaaaat gaaaatacgt tcgcaagtcg gcatggtgct taacctcgat aaatgtatcg
     5701 gctgccatac ctgttcggtg acctgtaaca acgtctggac cggacgcgaa ggcatggagt
     5761 acgcatggtt taacaacgtc gaaaccaaac cgggcattgg ttatccgaaa aactgggaag
     5821 atcaggaaga gtggcaaggc ggctgggtcc gcgatgtgaa tggcaagatc cgtccgcgtc
     5881 tgggcagcaa gatgggcgta ataaccaaaa tcttcgccaa cccggtggtg ccgcagcaga
     5941 ttgatgatta ctacgaacct ttcaccttcg actacgaaca tttgcatagc acaccggaag
     6001 gcaaacatat ccctactgct cgcccgcgtt cactgattga cggcaaacgg atggacaaag
     6061 tgatctgggg cccaaactgg gaagaactgc tgggcggcga gtttgaaaaa cgtgcccgcg
     6121 accgcaactt cgaggccatg caaaaggaga tgtacgggca gtttgaaaac accttcatga
     6181 tgtacctgcc gcgcctgtgc gaacactgcc tcaatcccag ctgcgtggcg acctgcccaa
     6241 gcggcgctat ctacaaacgc gaagaagacg gcattgtgct gattgatcag gataaatgcc
     6301 gtggctggcg tttgtgcatt agcggttgtc cgtacaaaaa aatctacttc aactggaaaa
     6361 gcggcaagtc agaaaaatgt atcttctgtt atccgcgaat tgagtcaggc caacccaccg
     6421 tgtgctcaga aacctgcgtg ggtcgcatcc ggtatctggg cgtgctgctt tacgacgccg
     6481 accgcatcga ggaagcgtcg agcaccgagc gcgaagttga cct
//
LOCUS       DAETQL010000161         6321 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.164, whole genome shotgun sequence.
ACCESSION   DAETQL010000161 DAETQL010000000
VERSION     DAETQL010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.164"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            73..378
                     /locus_tag="K0W65_003932"
     CDS             73..378
                     /locus_tag="K0W65_003932"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3096994.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="K0W65_003933"
     CDS             complement(454..918)
                     /locus_tag="K0W65_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBI3096995.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="K0W65_003934"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="K0W65_003934"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBI3096996.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="K0W65_003935"
     CDS             complement(1787..2476)
                     /locus_tag="K0W65_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBI3096997.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="K0W65_003936"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="K0W65_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBI3096998.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="K0W65_003937"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="K0W65_003937"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBI3096999.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="K0W65_003938"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="K0W65_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBI3097000.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1609 a         1709 c         1565 g         1438 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcaggatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc t
//
LOCUS       DAETQL010000162         6190 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.165, whole genome shotgun sequence.
ACCESSION   DAETQL010000162 DAETQL010000000
VERSION     DAETQL010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.165"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..95)
                     /locus_tag="K0W65_003939"
     CDS             complement(<1..95)
                     /locus_tag="K0W65_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3097001.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYL"
     gene            568..1476
                     /locus_tag="K0W65_003940"
     CDS             568..1476
                     /locus_tag="K0W65_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HBI3097002.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMLPGSTVPFISTK"
     gene            complement(1522..3513)
                     /gene="tkt"
                     /locus_tag="K0W65_003941"
     CDS             complement(1522..3513)
                     /gene="tkt"
                     /locus_tag="K0W65_003941"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBI3097003.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3791..4549
                     /gene="loiP"
                     /locus_tag="K0W65_003942"
                     /gene_synonym="yggG"
     CDS             3791..4549
                     /gene="loiP"
                     /locus_tag="K0W65_003942"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HBI3097004.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4755..5675)
                     /gene="speB"
                     /locus_tag="K0W65_003943"
     CDS             complement(4755..5675)
                     /gene="speB"
                     /locus_tag="K0W65_003943"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBI3097005.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5811..>6190)
                     /locus_tag="K0W65_003944"
     CDS             complement(5811..>6190)
                     /locus_tag="K0W65_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097006.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1530 a         1615 c         1595 g         1450 t
ORIGIN      
        1 cagagatagc gctgatgtcc ggcagtgctt ttgccgttac gcaccacgcc ttcagtagcg
       61 gagcaggaag gacatctgat ggaaatggaa gccacgcaag caccttaaaa tcaccatcat
      121 acactaaatc agtaagttgg cagcattacc ctgagattga tgattctgga aaataatcac
      181 taagccgcat aaattatcct tattttattt ttagggtacc gtccggcggt aaaaataaaa
      241 tactcgtgcg gtttaatgaa agcaattggg tacgcgagaa gaaaaacccc acaaaccaaa
      301 ataataaaac cttaaataac aacaatatga aattgatatt tttaaagtca actttgtatt
      361 tgttatgttc atcacaaaat caacattttg tttgttattg tatttgtgat gcatatctct
      421 atttatttat tgaatgttcg aaaacatact tttctaatgc aatgtttttt cgctataccc
      481 ctttatagat tctgaattct cacctgtcgg ctgttttctc ctcatctcat gtgacatatt
      541 cccatacttt actcaaggaa gattgtcatg aaagcacctg tttcattaac ctcatttcgc
      601 ccacaaaaat ctcttgctat cgccattggc gtgcttgccg tggttgtgct gccatttctc
      661 tcgtattaca ccgttaatga aggtgaacgc ggcattttgc tgcgctacgg aaaaatcgta
      721 aaagttgccg aaccggggct gggttttaag atccctttta tggaaagcgt ggagaaaatt
      781 tccacgcgta atcaggctgt ggtctatcag ggattacagg cctacagccg cgaccagcaa
      841 ccggcacaaa tgacagtatc agtcagcttc cacattaaac cttcagaagc aggcgcggtt
      901 tataccacct acaacactat tgaagcatta aaagatcgtc taattgtccg tcagttgcca
      961 actcagctgg agaacatttt cggccaatat accgccatca gcgccgtaca ggatcgtaca
     1021 aaattggttc aggatctgca aaatgccatg cgcaaagccg ttgttgggcc agtggtaatt
     1081 gacggggtcc agattgaaaa tattgatttt tctgacgctt atgaaaagtc gattgaaaac
     1141 cgaatgaaag cagaagtcgc cattgccacg cgtaagcaaa atctggaaac agagaaaatc
     1201 caggcgcaaa tagcggtaac tcaggcccag gcagaagcag acagtaaact ggcggcagcc
     1261 aaagcagaag cggaaactat tcgcgtaaga ggcgcggcag aggctgaaac tattcgttta
     1321 aaaagtgcgg ctgaagcaga agcaatccgc ctgcgcggtg aagctctgcg ggataatccc
     1381 ggacttgtag ccctcaccac tgccgaacgt tgggacggta aactgcccga taccatgctc
     1441 cccggcagca cagttccgtt tatctctact aagtaaaaaa agggccgctt tcgcggtcct
     1501 tctcacaaaa cgtcttactg attacagcag cgcttttgct ttcgcaacaa cgttatcaac
     1561 agtgaagccg aactcttcaa acagcagctc tgccggagca gattcaccga aggtggtcat
     1621 gccgacgata gcgccgttca ggccaacata cttgtaccag tagtcagcaa tacccgcttc
     1681 taccgcaacg cgtgcagtaa ccgctttcgg cagtacggat tcacggtaag ccgcatcctg
     1741 cttgtcaaat gcgtcggtag acggcatgga caccacgcgc gctttcacgc cttcggcagt
     1801 cagtttttcg taggcagcaa ccgccagttc aacttcagaa ccggtggcga tgaagatcag
     1861 ttccggctga ccagcacagt ctttcagcac ataaccaccg cgcgcgatgt ttgccagctg
     1921 ctcttcagta cgttcctgct gcgccaggtt ctgacgggag aggataagcg cagtcgggcc
     1981 gtcctgacgc tcaacgccgt atttccacgc gaccgcggat tcaacctggt cacacggacg
     2041 ccatgtagac atgttcgggg tcacgcgcag agaagcgacc tgctctaccg gctggtgagt
     2101 cgggccatct tcgcccagac cgatggagtc gtgggtgtaa accatcacct ggcgctgttt
     2161 catcagcgca gccatacgta cggcgttacg tgcgtattcc acgaacatca ggaaggtgga
     2221 ggtgtacggc aggaagccac cgtgcaggga gataccgtta gcaatcgcgg tcataccgaa
     2281 ctcgcgaaca ccgtagtgga tgtagttacc tgcagcatct tcgttgattg ctttagaacc
     2341 agaccacagg gtcaggttag acggtgccag gtcagcagag ccgccgagga attcaggcaa
     2401 cagcgggccg aacgcttcga tagcattctg cgacgcttta cggctggcga ttttcgccgg
     2461 attagcctgc agtttagcga taaactcttt cgctttggcg tcgaagtcag acggcatttc
     2521 gcctttcata cggcgggtaa attcagccgc ttcctgcgga taagctttcg cgtaagccgc
     2581 aaacttctca ttccatgcag attctttcgc ctggcctgct tctttcgcat cccactgagc
     2641 atagatttca gacgggattt cgaacggtgc atatttccag cccagctgtt cgcgggtcag
     2701 ggcaatttca gcgtcgccca gcggcgcacc gtgggagtcg tgagtaccgg ctttgttcgg
     2761 ggaaccgaaa ccgatgatgg ttttgcacat cagcagggac ggtttgtcag tcactgcgcg
     2821 cgcttcttca actgcgcgtt tgatggatgc cgcgtcatga ccgtcgatgt cgcgaataac
     2881 gtgccagccg taagcttcga aacgcattgc tgtgtcgtcg gtgaaccagc cttcaacgtg
     2941 accatcgata gaaataccgt tgtcatcgta gaacgcaatc agtttaccca gcttcagcgt
     3001 acccgccaga gagcaaactt cgtgggagat gccttccatc atgcagccgt cgcccatgaa
     3061 ggcgtaggtg tagtggtcga caatgtcgtg gcccggacgg ttaaactgcg ccgccagcgt
     3121 tttttctgca atcgccatac cgactgcgtt ggcaataccc tgacccagcg gaccggtggt
     3181 ggtttccaca ccagcggtat aacctacttc cggatggccc ggagttttag agtgcagctg
     3241 acggaagttt ttcagttctt ccatcggcag atcgtaaccg gtgaggtgca gcaggctgta
     3301 gatcagcatg gagccgtggc cgttggacag cacgaagcgg tcacggtcag cccaggacgg
     3361 attctgcggg ttgtgtttca ggaaatcacg ccacaggact tcggcaatgt cagccatacc
     3421 cataggggca cccgggtgac cggatttggc tttctgtact gcgtccatgc tcagcgcacg
     3481 aatagcattg gcaagctctt tacgtgagga cattttgact ccagatcgga tgatgaaggg
     3541 cacgccctta acgacttgac gacagcgcgt tttgggctac gccggaaaat ttgccaacaa
     3601 tttaccgcaa gccgcgcgtc atgtacatgg aacatccttt tgccgcttca gaaatctctg
     3661 gatcatgctc gcatgttgcg caatctgctc gcccgtccgc tgcgcttttc cttatactga
     3721 gactgagcgt cgattcacct gcaaacggcg catttttaga ataatcctga ccttgtgcgg
     3781 aagagaaaac atgaaaattc gcgccttatt ggtagcaatg agcgtggcaa cggtactgac
     3841 cggttgccag aatatggact ccaacggact gctctcatca ggagcggaag cttttcaggc
     3901 ttacagtttg agtgatgcgc aggtgaaaac cctgagcgat caggcatgtc aggagatgga
     3961 cagcaaggcg acgattgcgc cagccaatag cgaatacgct aaacgtctga caactattgc
     4021 caatgcgcta ggcaacaata tcaacggtca gccggtgaat tacaaagtgt atatggcgaa
     4081 ggatgtgaac gcctttgcaa tggctaacgg ctgtatccgc gtctatagcg ggctgatgga
     4141 tatgatgaca gataacgaag tcgaagcggt gatcggtcac gagatggggc acgtggcgtt
     4201 aggccatgtg aagaaaggga tgcaggtggc gcttggtaca aatgccgtgc gagtagctgc
     4261 ggcctctgcg ggcgggattg tcggcagttt atctcaatca caacttggtg atctgggcga
     4321 gaaattagtc aattcgcaat tctcccagcg ccaggaagca gaagccgatg attattctta
     4381 cgatcttctg caccaacgcg gcatcagccc ggcaggtctt gccaccagct ttgaaaaact
     4441 ggcgaagctg gaagaaggtc gtcaaagctc aatgtttgac gaccatcctg catccgccga
     4501 acgtgcccag catattcgcg atcgcatcag cgcggatggg attaagtaaa gcctagtggt
     4561 gtcgaacgta ggtcagataa ggcgttcacg ccgcatctga cattaatggc acatttgtta
     4621 ccttgtgcgc attgccggat gcgatgctgg cgcatcttat ccggcctacg ggtgccgaac
     4681 gtaggtcgga taaggcgttc acgccgcatc cgacattaat ggcacgtttt acccgtgcgc
     4741 atcgcatctg gtgcttactc gccctttttc gccgcctgaa tatacagcat ctccagcgcc
     4801 agcgtcgccg cagccagagc ggtgatttcc gactgatcgt atgccggagc cacttccact
     4861 acgtccatcc caacgatgtt gagatctttc aggccgcgta ccagtttaat ggcgcgatcg
     4921 gaggtcaggc cgccaatcac tggcgtaccg gttcccggcg cgaaagcagg atccaggcag
     4981 tcgatatcaa aagtcaggta aaccggcata tcacccacaa tctgtttcac ctgggcgata
     5041 acgtcatcca cgctgcgatc gttcacctgg caggcgtcca gcacggtaaa gccgttgtct
     5101 ttatcaaact cggtacgaat accaatctgc acggaatgat tcgggtcgat cagaccttct
     5161 ttcggcgcgg tatagaacat agtgccgtgg tcaaattcac aaccgttcgc ataggtatcg
     5221 gtgtgggcgt caaagtgtac cagcgccatt ttgccgaaat gcttcgcatg agcacgcagc
     5281 agcggcagcg taacaaagtg gtcaccaccg aaggagagca tacgcttacc ggcagccagc
     5341 agcttctcgg cgtgcgcctg cagcttttcg ctcatctcac gggcatcgcc aaaggcatat
     5401 accagatcgc cgcagtccac gacgttcaga cgctcacgca tgtcgaaatt ccacgggaag
     5461 cggttgtgtt cccaggccag attcgtcgaa acctgacgga tcgctgccgg accgtggcga
     5521 ccacccgcac gaccagaagt ggccatatcg aacggcacgc cagtaatcac ccagtctgca
     5581 tcgctgtcat acggctggaa gttcatcggc aggcgtaaaa aaccaaaggc attggaaacc
     5641 agtgagttat cgtattgatg acctaaggtg ctcatggatg gactcctatt acaaagtcga
     5701 tataaaaaaa cccttccgcg tcgttaggcc cgacgaggaa gggttggatt tgcgaaacac
     5761 aagtttgtgc taattatcgc cttaaatggg cgaggcatca agcgaggttg ctatttccga
     5821 cacggtggcg gcgcatagcc attgttatct gccggaaagg cttgtccaga gcacggcggc
     5881 agcgcgtttt gcgccgggcc ttgtaattca aaggcggcaa agccgattac cccggtatta
     5941 cgcacatcgc cttgcgccga attggctgcg taggaatcag caacgttggc gaactgaaaa
     6001 gccgcttcag tatgtttatc tttgcggaat cctttaatca ctaacgaatc tccggcattc
     6061 acaatatagc cgttgttatt cagcgaacct tgcttgccgt tcagcacatc caggccatca
     6121 gccgtagcaa cgatctcgta gttggtgtta cgactgtaat tacggacata taattgataa
     6181 ttcattccga
//
LOCUS       DAETQL010000163         6058 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.166, whole genome shotgun sequence.
ACCESSION   DAETQL010000163 DAETQL010000000
VERSION     DAETQL010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.166"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..143)
                     /locus_tag="K0W65_003945"
     CDS             complement(<1..143)
                     /locus_tag="K0W65_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HBI3097007.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="K0W65_003946"
     CDS             379..651
                     /locus_tag="K0W65_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBI3097008.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="K0W65_003947"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="K0W65_003947"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBI3097009.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1731
                     /locus_tag="K0W65_003948"
     CDS             1111..1731
                     /locus_tag="K0W65_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBI3097010.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="K0W65_003949"
                     /pseudo
     CDS             complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="K0W65_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3114..3767)
                     /locus_tag="K0W65_003950"
     CDS             complement(3114..3767)
                     /locus_tag="K0W65_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBI3097011.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3767..5238)
                     /locus_tag="K0W65_003951"
                     /pseudo
     CDS             complement(3767..5238)
                     /locus_tag="K0W65_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5211..6058)
                     /locus_tag="K0W65_003952"
                     /pseudo
     CDS             complement(5211..6058)
                     /locus_tag="K0W65_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1621 c         1599 g         1411 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggatc ttcggtgcgt tgtgctggat gacgaccttt
     1681 acgcgggtgt ggagtgggta tctgacgctg aagtcgctcc agcgtcagta attgcaatgt
     1741 tatttgctac tgcccgggcc gcgttcaccg gttgcaaccg gattttttgg atcgctgctc
     1801 cattcgtacc agccgccgtc atacacggaa acattcttcc aacccatggc gcgtgcgtac
     1861 ataaaggttt cggacgcgcg ccagccggtg ccgcagtaga atgaaacttg ctgatctggt
     1921 ttgatattcc atgctttcca catagcggta atatcatcgg cgctacgcat ggtgccatcc
     1981 gggttataga aatcttccat atgcgtcgag tcgctaccag cgtgtcccca acgtgctccg
     2041 gctatttcac cttttggttt aatatagctg taaccgctgg tcgtaccgat aaattctggc
     2101 cacgaacgaa tgctcaccag cgatgcatcc tggcgatgca gcagtccacg ggcctgttcc
     2161 atatcgagca tcaactgcgg ttgtgccggg atcttcacgc cgaaatccgg ttccgctttc
     2221 actttcggtg gcgttcctcg ctcaacaggc agtcccgcgt cggaccaggt ttgccagccg
     2281 ccatccagca gacgcacatc tttcacgcct gcgtagagca taatttgcgc cacacgcgct
     2341 ccagcgtata cgtcacgccc gtacagaatg accgtggtgt catggcgaat gccgtgtttt
     2401 gccagcatcg cttttagttg ttcatcagaa actttgttcc acagcggttc gctttccact
     2461 tcgttggtat cgatgtagtc agcgccagga atatggctga taaggtaaag cttaggagcg
     2521 ccctaggccg cttcaatgac tttccagtca ccgacaggtt tcgccgtaac ctctttacct
     2581 tgttgcaggt catgcagcca ttgcggataa accagctgct caaagtgcgg cagtttttgt
     2641 agacgggaag gttcgcttag cgcgtcactc agaatggaga tatgcgtaaa acctgctttt
     2701 tgcagtcgcg ttttgacggc gtcaacatct ttgccattac cgtacagcgc caccggggca
     2761 tcggctttca ggttatgttg cttgatccac gcgttgagct gttcggtgct cattttgtcc
     2821 agccagctag cagagaggtt taaggcggca ggttcatgac cagaagggcc atttaaggtt
     2881 tgtggccagc cgttataaaa cgcgctgggg cgagtatcta tcgctttgcc attttgttgc
     2941 tgaagttggt caagtgtaag aggcttcgcc agttcagcgg cccacgaaga agcgcaagcc
     3001 agccctaaag ccattgccag cgcggtcatt tgagaaacac gtttcatcga aaatcccgac
     3061 attgtagttt tgagtgcgcc attctgaact gacgcattga gaaaaacttt gcgttatcgc
     3121 agtttgttgt aattttctga ccactgcgcc atatccagaa cagaactatc agcaggaaca
     3181 tcctggagat cgtgcgttac ctgaacgacg gggatcgcca gggcgcgaac ttcgctgaac
     3241 acccactggc gaaaattatc gcgcagagcc acatcaagac ggctgaatgg ctcatccagg
     3301 agtaacgctt ttggctgggc gagaagggcg cgtagtagag caacgcgcgc tcgctgaccg
     3361 ccagacaaag tggcaggatc ctgatggaaa gctccgtcaa ggcctgaacg ctcaagtgca
     3421 tcattcacgg cattacgtcg ggcattcccc ttaagtgtcg ccggtagcgc cagcagtaaa
     3481 ttttgcccga cgctgaactg gtcaaataac agtgcatcct gaaaaagaat gccaatctga
     3541 cgctgtgcgg tgggtagcat gtcaatccgt tgctcattga gccatagctc acctgtacaa
     3601 gaaaactgtc cggccagtgc accaatcatc catgaaaaca gagtggattt tccacagcca
     3661 gacggcccca ttaacgtgac gatgtcacct ttatcaaccg taaagttaac gtttgtcagc
     3721 aagcggcttt ctggtaaacg tagcgaaaca tttttcacgc agagcattag cggattcctt
     3781 gtctgacata acctacccat tttgcgacta acgcggtcag ggcaaaaata ataagcggta
     3841 acaacagttg ccataacgcc tgggcggcga gaataccgtt gctgccgccg ctgcttaatg
     3901 ccaccgcctc agtggtgagc gtcggaaaac gccccgcgcc cagccacagc gtcggcatat
     3961 actgcgcaat actgactgaa aatcccaccg cgaaggcaat cagcacaggg cgcaacatga
     4021 gtgggcattt cacgtaaaag aagattttgg cccgcgacca gcccagtgtt tgcgcaatca
     4081 aaattaaccg tgaatcaatg cgctgccagg cgggttgcag gataaacagc atccacggca
     4141 ttacccacag cagatgcccc cagaccaccg ccgtccagct tccatccagg ttcagccata
     4201 gcgccagcgt gtactggccc gccaccagtg gcagagcggg caatagaatg ggcaaccata
     4261 gccacaactg gcgacgctgt gccccccatt ccagccacag cagtaacagg agcaaagcga
     4321 tgaatgttgc taccagcccc attgtcagac tgttgatgag tgcttcacta ttgatcgtcg
     4381 actgatccgc gaggatcgcc agcagaacca cacacagcac accggttaag ggtaaaaaac
     4441 tcgccagcgt agtgcctggt aataaaggtg tggcaggctt gcgaatgcca tctacgcggg
     4501 gaatagtgcg ccgccagctg cgccaaaacg taggcggcga gtaacagcat cagcagcaga
     4561 ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct gagttaacca ctgccagcta
     4621 attactgcca gcgtcggtgg attacctggc ccaagaataa tcgccacatc cacgaccgaa
     4681 agcgaccagg caacaatcgc cagcatcgcc attgccagcg gctgtagccc agcgaatcca
     4741 gcacaatgac ctgctgcaac agccgttttt cgctcaatac tgccactaag atccacagca
     4801 gaaatgcgct ttctttcact gcaagggtga ggcccagacc gatccaaatc ggtccattgg
     4861 cggagtgaaa tacgggaaat agtcatatag cagccctccg tcagcaaaga gcagcagggc
     4921 gctggtggca aaagccacat ggggaatggc gagcagccac ggcagacggg cgcacatacg
     4981 ctgccatttc ggcccaggcc atagcgccac aatcaccagc agggcaatca acaatgcccc
     5041 gaccgccgcg atggttgtcg acaccagcgt tgccagtaat gcctgcggta actgtggatc
     5101 ggcaaataac gcctgccagc gtgtcaacga caacgcaggc gatatcagcg tgagagctgc
     5161 cgggatcagc ggtgcataaa tcaccgccac catcgcccac agcaaaaaga ttaatgcgta
     5221 ccgtaacggc gtaaccattc ttgttccagt gcattcaccc atcctgcgtg cggttcagcc
     5281 agtaccggcg gcagatcctg cggcattctt gattgcaata tttcgcgctg cccgtcaggc
     5341 agtttttgcg gatcgagaac agaaggatcg ccccagacaa cgggatctgc tttacgcagt
     5401 tgcgcatcgg gtgagagcag gaaattggca actaccttcg ccgcagcact ggcattcgcg
     5461 ttggctggaa tggtgacgaa atgcacgttg ccaatcatcc cctcgcgaaa gccaaaactg
     5521 taactgcttg ccggcaaatc gccgctggcg attttttgct gcgcatgtgc ggggttaaag
     5581 gtcagcgaca ggcgcaatgt gccggctttc agcagagcat ccatccgcgc gggcgaaggc
     5641 gggaaatcct ttccttcgcg ccacaaatac ggatgcagtg catcaagata ttgctacaag
     5701 ggcgcagtga cacgggcgaa agtcgcatcg tccggcgctt cttttaatgc ggcgggatct
     5761 ggcgtcagca taatcagcaa ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc
     5821 ggataagtaa ccgtgccggg attggcttta gcaaactcca gtaaggcttg cggcgtttgt
     5881 ggtggctgtg gcgtaacatc gcggcgggcg ataaacgtca gttgtgcgcc gccccagggc
     5941 gattccgcac cttgtgtcgg cactgaaaaa tctccccgca ccggcagctg tgtgtcgaca
     6001 tagcgccagt tgggcagagt ctccgcccag cccgtttgta ataaattggc ctctttta
//
LOCUS       DAETQL010000164         6017 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.167, whole genome shotgun sequence.
ACCESSION   DAETQL010000164 DAETQL010000000
VERSION     DAETQL010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.167"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            66..584
                     /gene="mddA"
                     /locus_tag="K0W65_003953"
     CDS             66..584
                     /gene="mddA"
                     /locus_tag="K0W65_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBI3097012.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            581..1030
                     /locus_tag="K0W65_003954"
     CDS             581..1030
                     /locus_tag="K0W65_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBI3097013.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1031..1264)
                     /locus_tag="K0W65_003955"
     CDS             complement(1031..1264)
                     /locus_tag="K0W65_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HBI3097014.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1350..1523)
                     /gene="ortT"
                     /locus_tag="K0W65_003956"
     CDS             complement(1350..1523)
                     /gene="ortT"
                     /locus_tag="K0W65_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HBI3097015.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1718..1813
                     /gene="yncL"
                     /locus_tag="K0W65_003957"
     CDS             1718..1813
                     /gene="yncL"
                     /locus_tag="K0W65_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBI3097016.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2215..3024)
                     /locus_tag="K0W65_003958"
     CDS             complement(2215..3024)
                     /locus_tag="K0W65_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBI3097017.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3234..4658)
                     /gene="patD"
                     /locus_tag="K0W65_003959"
     CDS             complement(3234..4658)
                     /gene="patD"
                     /locus_tag="K0W65_003959"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HBI3097018.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4680..5474)
                     /locus_tag="K0W65_003960"
     CDS             complement(4680..5474)
                     /locus_tag="K0W65_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBI3097019.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5464..5811)
                     /locus_tag="K0W65_003961"
                     /pseudo
     CDS             complement(5464..5811)
                     /locus_tag="K0W65_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT         1565 a         1530 c         1347 g         1575 t
ORIGIN      
        1 cgttgcttcg ggctgttttc gatagtattg ataacatcat catcattatt ttattgaggc
       61 cagacatgtc catccgtttt gcccgcaaag ccgactgtgc tgccattgcg gaaatttata
      121 accacgccgt gttgtatacg gcggctatct ggaatgacca aacggtggat gcagataacc
      181 gcattgcctg gtttgaagcg cggaccatag caggttatcc ggtgctggta agtgaagaag
      241 atggcgtagt tacgggatat gcctcgtttg gcgactggcg tagtttcgat ggttttcgcc
      301 ataccgtgga acattcggtt tatgtccatc ccgatcatca gggcaaaggt ctggggcgta
      361 aattgttaag tcgattgatt gatgaagcgc gggattgcgg gaagcatgtc atggtcgccg
      421 ggatcgaatc gcaaaatcag gcttcgctgc atctgcacca gtcgctggga tttgtcgtca
      481 ccgcacaaat gccgcaggta ggcactaaat ttggtcattg gctggatctg acatttatgc
      541 agttgcaact cgacgagcgc actgaaccgg acgcgattgg atgaatcagt cgctcaccct
      601 tgcttttttg attgccgccg gaattggtct ggtagtccag aacacgctga tggcgcgcat
      661 cacccagacc tcctctacca ttctcatcgc catgttgctg aactcactgg tggggattgt
      721 gctctttgtt tctattttat ggtttaaaca aggcatggcg gggtttggcg aactggtgtc
      781 cagcgtgcgc tggtggacac ttatacctgg cttgctgggt tcgttttttg tcttcgccag
      841 tatcagcgga tatcagaatg tgggagcggc gacaactatt gcggtactgg tcgccagcca
      901 gctgattggc ggtctggtgc tggatatctt ccgcagtcat ggcgtaccac tacgggcgtt
      961 gttcggccca atatgtggcg caattttact tgtggttggc gcctggttgg tggccagacg
     1021 ctcattttaa tcagaggatg gtgccgcctt tggttaactg ttcgtggcgc gcttccatat
     1081 cttctttatg cttgcgaccg tgatgagcaa tagcggtgcg caaacgctgc tgctgggtgt
     1141 agcgatcttc ccgacttaac tccgcgtcat cgctcagggc gattaataat tcgttcatat
     1201 gggcaatgac gctctcttca atcgcggcat ccacgcgcgc gatgacttca tccagatgcg
     1261 acatactttc tccttagcca cttgtgaaat ttatcgatta atccaaatga tataagtctg
     1321 cgtggtgccg tcaagccagt gagtaagatt taaaacagtg aaaacaacag cgccaccggg
     1381 aaactcatcg gccatgtggc ccccaccaga aaggcgctta agaaacggat gcgtttctta
     1441 tcgtgagaaa gaaaccaggt gataagaaat gcgattgctg ccataaccgc ataaaaaaca
     1501 agcatatgtt gatagagaaa catgtacggg cgtccagcca tatgaataaa acgcgcgcaa
     1561 atatgcgcca ttttttgacg tgcatcaagt tttttcacca gtttgagaaa aaatcatcaa
     1621 acagcaactt gcccttatcc atcttcagaa tgagtattat tgcggatttt cttaacccgt
     1681 actatacaca taggggcaat gataaaaggt ggcaaaaatg aatgtttcca gtagaactgt
     1741 agtactgata aatgtctttg ctgctgttgg tttgtttact cttatctcta tgagatttgg
     1801 ctggtttatt tgatgtagtg attttgcacg cagcacgtac tgtcagagaa atcaactctc
     1861 tgacagtacg caagatcttc tcatactctc caagtttgca acattatcct taatatttac
     1921 ttctttttgc tggcatctac attgaaatga gtagaaaaca tccccctact gtcgtctata
     1981 caaacagctt actgattcaa acggaatctt gttttcacat ttttctatca cgaatacttt
     2041 atacgcgcgg gagtctataa atgctttata ctttcaagga ataacgtaaa tataaaaaga
     2101 tggcgtaaag tatatgcatg gcaggatgtt tgcaacctcc ttgatgacgc tgccccattg
     2161 aaattgcaaa ccccgccctg gttatcttgc ccatttatca tgcaaaacag ataattatag
     2221 gcctctgaat acctcagaaa gattgaattt tttatagtcc ctctcttgaa tatttccatt
     2281 tgatatcaat cgattcatct cttcaacaaa ataatttata tcatcacact tattattatc
     2341 cggaagatct gagtagttat aattccacca ttttattctc aacagttcat caataacatt
     2401 tgaatcaaac ctaaatctga ttatttttgc aggaactcct gcaactatgg catatggggg
     2461 cacatctttg gtaacaacgg aattggcagc aataacagca ccatcaccta ttgcgatgcc
     2521 acctttcaat acaacatcat ttcctatcca gacatcattt cctataatcg gattttcagg
     2581 tttggtaatg aatggtttca atgtccagct acgttcaaat tcactcatag caaacttatc
     2641 aaattcaccg ttataagtca tcatgtgagt agtaaaacga ttcaaaggat gatttccacc
     2701 cattattttt acatttgatg caatactaca aaatcgacct atacgtatat tccctgaaaa
     2761 atggcaggtc ggaaatgaaa aagcgccgac actggagaaa tttttcacag gcattgttga
     2821 atattcttca gcaataacat tatcttcaat tgttatcttg tcaccttttg agaaaagaaa
     2881 taaattcctt tctttatacg acttcttccc cttatgattt aaaaagattt tattatccat
     2941 acaaaactca aagtgttttt tcgtccatct gaaggtaagg gttttatttt tatcttccgt
     3001 gaatttgtac aattgaactt tcattgttaa tacttctact tataaagtat tagtttaata
     3061 acgatactct aaagccttat gctaatcaac tgtgacagga tgaaaaatcg ccatcaaaca
     3121 tttagcctaa tttttacgcc agttattaat gtaatcattc taatacttaa ccgccattaa
     3181 actcagaacc taaaacgata gtggccgtcc tataacagcc actaacatct aacttaatgt
     3241 ttaaccatga cgtggcggac gacggtgtaa tcctccagcc cataaagtga catatccttg
     3301 ccgtaaccag aaagtttctg cccaccgtgc ggcatttcac ttaccagcat gaaatgggta
     3361 ttgacccagg tacaaccata ttgcagccgt gcgctgacgc gatgcgccct gcccacatct
     3421 ttcgtccata ccgaagatgc aagtccgtac tggctgtcat tcgcccagtt caccacctgt
     3481 tcttcgttgt cgaagggcgt aacactcact actggaccaa atacctcttt ttgcacgatg
     3541 gcatcgtcct gtaatgcgcc agccagcagc gtcggcgcat agtaatagcc attacccttg
     3601 cgcttttcac cgccagtgat cactttgatg tgccctgtcg ctttcgcctc ttctactgcc
     3661 ttgctgacgc gttcgagatg cgccagcgag cttaaaggtc caagctccgt agactcgtca
     3721 tccggcgcac cagattttaa cgttgccacc gcagcaccca gtttttccac cagcgtatcg
     3781 taaatgcctt tttgcgcgta gatccgacaa gccgcagtac aatcctgtcc agcattgtaa
     3841 tagccaaatg tacgtacacc ttcgaccact gcttcaatat ccgcatcatc aaaaacaatc
     3901 actggcgctt tgccaccaag ttccatatga gtacgcttaa tggacgacgc ggtatggctg
     3961 atgatgtgct cgccggtggc gatagagccc gtcagcgaca ccatccgcac tttgggatga
     4021 ccggtcagcg gatcacccac cgttttgcct ctgccaaaca gtatgttaat cacgcctgcc
     4081 gggaagatat ctttcgccag ctctgccaac ttcaacgcgg tcagcggggt aatttctgat
     4141 ggtttaagca ctacgcagtt ccctgccgcc agcgccggag caagtttcca cgcggccatc
     4201 atcagcggat aattccacgg tgcgatagaa gccacgaccc ccaacggatc gcgacggatc
     4261 atcgaagtat gaccttcaag atattcacct gccgccagac cattcagaca gcgcgccgca
     4321 cccgcgaaaa agcgaaaaac atcgacaatc gccgggattt catcattgaa cgcactatgc
     4381 agcggtttgc cacaattacg ggattccagt tcggcaaaaa cctgaccatt ttcttcgata
     4441 acatccgcca gtttcagcag gcattccgca cgcgctttcg gtgtggtttg cccccattca
     4501 gcaaatgctg catctgccgc acgcacagca gcatcgacct gctctgcgga tacctcggca
     4561 atctccagta aaacgtcccc cgttgccgga ttatagacag gctgtttttc cccttcgccg
     4621 ctaacccgtt ctccgttaat cagtaactta tgttgcatag cttattcctg tatcagtggt
     4681 tattgaccat tgtcgccttc gcgggttagc caccaggccc ccaggatcgg caaggttgtt
     4741 accaacataa ccagcagtgc caccacgttg gttaccggta catcacgcgg tcgcccaagc
     4801 tgattgagca accacaacgg taacgttcgt tcatgacctg ccgtaaaggt cgtaacgatg
     4861 atttcatcga acgacaaggc aaacgccagc attcctcctg ccagtaacgc cgaactgaga
     4921 ttcggcaaca ctacgtagcg gaaggtttgc cagccattgg ccccaagatc cattgacgcc
     4981 tcaaccagac tccacgaggt gcggcgaaaa cgggcgatga cattgttaaa caccaccact
     5041 acgcaaaaag tcgcatgacc gaccacgatg gtgaaaaatc ccggctccag attgatggtt
     5101 ttaaaggcgg ttaatagcgc cagaccagtg acaatgcccg gcagcgcaat gggcagcagc
     5161 aataacagcg aaatggcgtt tttgccgaaa aagtctcgtc tccacagcgc ggcagctgcc
     5221 agcgtcccta acaccagcgc aattaatgtc gccagcgccg ccactttaag tgacagcgtc
     5281 acggcatcaa gaatatcact acgctgtgct gccacgctaa accagcgcag cgtcaggccc
     5341 tgcggtggaa aactaaacgc cgcatcttca gtgttaaacg cataggcggc gattatcagg
     5401 atgggaaaat gtaggaaaac aacgccgccc caggccgcca gtttgaggaa aaacggtgcg
     5461 cgttcagagt gcatcgaacg ctcccagacg tttcacgaac gccaggtaca gtgcgatgag
     5521 aataatcggc accagggtga atgccgccgc catcggcata ttgccaatcg ccccctgctg
     5581 ggaataaacc atattgccga taaaatatcc tggaggacca accagctgcg ggacgataaa
     5641 atcgcccagt gtgagtgaga aggtaaagat agagccagcg gcaatacccg ggattgccag
     5701 cggcagcacc acatagcgaa aggtttgtcg tggacgtgcg ccgagatcag ccgacgcctg
     5761 caacaatgac ggcggcaaac gctcaagcgc cgcctgaacg ggcaggatca tcgagtaggc
     5821 agcctgacgg ctgcggcttg tcatggtctg caattaccgt tataaaaaca ggcaatgtca
     5881 ttgtctttca ggtggttata tggcccgttc agctaaaccc cgtaaacgta aacccgcctc
     5941 tcaaagttcc tgagtcgtga cccgttcgaa tgcgtgctgt gtggcggccg gatggtatac
     6001 cgccgggcca ttgcggg
//
LOCUS       DAETQL010000165         5983 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.168, whole genome shotgun sequence.
ACCESSION   DAETQL010000165 DAETQL010000000
VERSION     DAETQL010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.168"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..393)
                     /locus_tag="K0W65_003962"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="K0W65_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="K0W65_003963"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="K0W65_003963"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HBI3097020.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="K0W65_003964"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="K0W65_003964"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HBI3097021.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..>5983
                     /locus_tag="K0W65_003965"
     CDS             5797..>5983
                     /locus_tag="K0W65_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3097022.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1840 a         1355 c         1018 g         1770 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga aactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg agg
//
LOCUS       DAETQL010000166         5899 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.169, whole genome shotgun sequence.
ACCESSION   DAETQL010000166 DAETQL010000000
VERSION     DAETQL010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.169"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..411
                     /locus_tag="K0W65_003966"
     CDS             <1..411
                     /locus_tag="K0W65_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3097023.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="K0W65_003967"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="K0W65_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="K0W65_003968"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="K0W65_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HBI3097024.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
     gene            5722..>5899
                     /locus_tag="K0W65_003969"
     CDS             5722..>5899
                     /locus_tag="K0W65_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097025.1"
                     /translation="MLTRKSIDTVLLSVGAEKLSQREWDWMKMLKPMDPPPAMVAASI
                     LERRGDTAALTRLQD"
BASE COUNT         1446 a         1489 c         1408 g         1556 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc gagaaccacg gcagcctgac ggctgcaaat tgaaaactgc
     5641 ccctgttcgt cctggtgttg ccgcacgctc cgcagaatat gtatgctctg aggacctgtt
     5701 ttattaagga ctcatcctct tatgttgacc cgcaaaagta tagataccgt tctgctctct
     5761 gttggtgctg agaagctctc acaacgtgaa tgggactgga tgaagatgct caaacccatg
     5821 gacccaccac ctgcgatggt cgcggcctcc atcctggagc gccgtggcga cacagcagcc
     5881 cttacccggc tacaggata
//
LOCUS       DAETQL010000167         5889 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.170, whole genome shotgun sequence.
ACCESSION   DAETQL010000167 DAETQL010000000
VERSION     DAETQL010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.170"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            50..244
                     /locus_tag="K0W65_003970"
     CDS             50..244
                     /locus_tag="K0W65_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HBI3097026.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
     gene            511..1437
                     /gene="tauA"
                     /locus_tag="K0W65_003971"
     CDS             511..1437
                     /gene="tauA"
                     /locus_tag="K0W65_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3097027.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            1450..2217
                     /gene="tauB"
                     /locus_tag="K0W65_003972"
     CDS             1450..2217
                     /gene="tauB"
                     /locus_tag="K0W65_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBI3097028.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            2214..3041
                     /gene="tauC"
                     /locus_tag="K0W65_003973"
     CDS             2214..3041
                     /gene="tauC"
                     /locus_tag="K0W65_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBI3097029.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            3038..3889
                     /gene="tauD"
                     /locus_tag="K0W65_003974"
     CDS             3038..3889
                     /gene="tauD"
                     /locus_tag="K0W65_003974"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HBI3097030.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="K0W65_003975"
     CDS             complement(3995..4969)
                     /gene="hemB"
                     /locus_tag="K0W65_003975"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBI3097031.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5493..5861
                     /locus_tag="K0W65_003976"
                     /pseudo
     CDS             5493..5861
                     /locus_tag="K0W65_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1399 a         1454 c         1598 g         1438 t
ORIGIN      
        1 agaaatgttg tcgtttctta ccagtgagga acgagaagcc cttacaaaca tgctcgcaag
       61 gaaaggtgtt gagactgtgc tacgcttgtt agataaggaa actcaagagc tactgcaact
      121 agagggccgt aaacgcctgg cagcgttgat gctcgttgat atgcccgacg agcaagtcag
      181 agagattttg gaagagattg aagctagtat tctctcagag agtgtaaacg cgaaggcggg
      241 ttaacttcat tgacctgcct ttagggtgcc tttaaaattc gtttttaaag gctacgatga
      301 catttttgaa gagtcttaaa agaattaaaa tacacggcgc gcctatcttg aatgttttat
      361 cgatatcgaa gcattgagca tttttaactc gttttatctt gaatgcaatc ggcgcataac
      421 ttattgaatc ccatcaaatc ccgtcttttt ttacccattc ccggttatct tgaataaatt
      481 acctagtcat ataacacact tcttgccgca ctggcattca tcgcttttca ggcacaggcg
      541 gtgaacgtca ccgtggcgta tcaaacctca gccgaaccgg cgaaagtggc tcaggccgac
      601 aacacctttg ctaaagagag cggggcgagc gtggactggc gtaagtttga cagcggagcc
      661 agcatcgtgc gggcgctggc ttctggcgac gtgcaaatcg gcaacctcgg ttccagccca
      721 ttagcggttg cagccagcca acaggtgccg attgaagtct tcttgctggc gtcaaaactg
      781 ggtaactccg aagcgctggt ggtaaagaaa actatcagca aacccgaaga tttgattggt
      841 aaacgcattg ccgtaccgtt tatctccacc acccactaca gcctgctggc ggcgctgaaa
      901 cactggggga ttaaaccagg gctagtggag attgtgaacc tgcagccgcc cgcgattatc
      961 gccgcatggc agcggggtga tattgatggt gcttatgtct gggcaccggc ggttaacgcc
     1021 ctggaaaaag acggcaaggt gctgaccgat tctgaacagg tcgggcagtg gggtgcgcca
     1081 acgctggacg tctgggtggt gcgcaaagat tttgccgaga aacatcctga ggtcgtgaaa
     1141 gcgttcgcca aaagcgccat cgatgcacag caaccgtaca ttgctaaccc tgacgcgtgg
     1201 ctgaaacagc cggaaaacat cagcaaactg gcgcgtttaa gcggcgtgcc tgaaggtgac
     1261 gttccggggc tggtgaaggg aaatacctat ctgacgccgc agcaacaaac ggcagaactg
     1321 accggaccgg tgaacaaagc aatcatcgac accgcgcagt ttttgaaaga gcagggcaag
     1381 gtcccggctg tagcgaatga ttacagccag tacgttacct cgcgcttcgt gcaataaaag
     1441 gaggcgcaga tgctgcaaat ctctcatctc tatgccgatt atggcggcaa accggcactg
     1501 gaagatatca acctgacgct ggaaagcggc gagctactgg tggtgctggg gccgtccggc
     1561 tgcggtaaaa ccaccctgct gaatctgatt gccggttttg tgccttatca gcatggcagc
     1621 attcaactgg cgggtaagcg tattgaggga ccgggagcag agcgtggcgt agtttttcag
     1681 aatgaagggc tgctaccgtg gcgcaatgta caggacaacg tggcgttcgg cctgcaattg
     1741 gcaggtatag agaaaatgca gcgactggaa atcgcgcacc agatgctgaa aaaagtgggg
     1801 ctggaaggcg cagaaaaacg ctatatctgg cagctttccg atggtcaacg tcagcgggtg
     1861 gggattgccc gcgcgctggc ggcgaatccc cagctgttat tactcgacga accgtttggt
     1921 gcgctggacg ccttcacccg cgaccagatg caaactctgc tgctgaaact ctggcaggag
     1981 acgggcaagc aggtgctgtt gattacccac gatatagaag aagcggtgtt tatggcgact
     2041 gaactggttc tgctttcatc cggccctggc cgtgtgctgg agcggctgcg gctcaacttt
     2101 gctcgccgct ttgttgcggg agagtcgagc cgcagcatca agtccgatcc acaattcatc
     2161 gccatgcgcg aatatgtttt aagccgcgta tttgagcaac gggaggcgtt ctcatgagtg
     2221 tgctcattaa tgaaaaactg cattcgcggc ggctgaaatg gcgctggccg ctctcgcgtc
     2281 aggtgacctt aagcattggc acgttagcgg ttttactcac tgtatggtgg acggtggcgg
     2341 cgctgcaact gattagcccg ctgtttttgc cgccgccgca acaagtactg gcaaaactgc
     2401 tcaccattgc cggaccgcag ggatttatgg acgccacgct gtggcagcat ctggcagcca
     2461 gcctgacgcg cattgtgctg gcgctactcg cggcggtggt gatcggtatt ccggtcggga
     2521 tcgccatggg gctaagcccg acggtgcgcg gcattctgga tccggtaatc gaactttatc
     2581 gtccggtgcc accgctggct tatttgccgc tgatggtgat ctggtttggt attggtgaaa
     2641 cctcgaagat cttactgatc tatttagcga tttttgcgcc ggtggcgatg tcggcgctgg
     2701 caggggtgaa aagcgcgcag caggttcgca ttcgtgccgc ccagtcgctg ggtgccagcc
     2761 gtgcgcaggt gctgtggttt gtcattttgc ccggcgcgct gccggaaatc ctcaccggat
     2821 tacgcattgg tctgggtgtg ggctggtcta cgctggtagc ggcggagctg attgccgcga
     2881 cacgcggttt aggatttatg gttcagtcag cgggtgaatt tctcgcgact gacgtggtac
     2941 tggcggggat cgcggtgatt gcgattatcg cctttctttt agaactgggt ctgcgcgcgt
     3001 tacagcgccg tctgacgccc tggcatggag aagtacaatg agtgaacgtc tgagcattac
     3061 cccgctgggg ccgtatatcg gcgcacaaat ttcgggtgcc gacctgacgc gcccgttaag
     3121 cgataatcag tttgaacagc tttaccatgc ggtgctgcgc catcaggtgg tatttctgcg
     3181 cgatcaagct attacgccgc agcagcaacg cgcgctggcc cagcgttttg gcgaactgca
     3241 tattcaccct gtttaccctc atgccgaagg ggttgacgag atcatcgtgc tggataccca
     3301 taacgataat ccgccggata acgacaactg gcataccgat gtgacattta ttgaaacgcc
     3361 acccgcaggg gcgattctgg cagctaaaga gttaccttcg accggcggtg atacgctctg
     3421 gaccagcggt attgcggcct atgaggcgct ctctgttccc ttccgccagc tgctgagctg
     3481 gctgcgtgcg gagcatgatt tccgtaaatc attcccggaa tacaaatacc gcaaaaccga
     3541 ggaggaacat caacgctggc gcgaggcggt cgcgaaaaat cctccgttgc tacatccggt
     3601 ggtgcgaacg catccggtga gcggtaaaca ggcgctgttt gtgaatgaag gctttactac
     3661 gcgaattgtc gatgtcagcg agaaagagag cgaagccttg ttaggctttt tattcgccca
     3721 tatcactaaa ccggagtttc aggtgcgttg gcgctggcaa ccgaatgata ttgcgatttg
     3781 ggataaccgc gtgactcagc actatgccaa tgccgattac ctgccacagc ggcggattat
     3841 gcatcgggcg acgatccttg gggataaacc gttttatcgg gcggggtgat acgagtgcgg
     3901 acggttccct cgcccccttg gggagagggt tagggtgagg gggcgttcac tgtactttca
     3961 acaggttaac tccccctttc tgagaggaaa aaaattaacg cagaatcttc ttctcagcca
     4021 aatccagcgc aaagtagctg aaaatcagat ccgcacccgc acgcttaatt gaacctaagc
     4081 tttcgagcac gactttctct tcatctatag cacccgccag cgcggcgaac ttaatcatcg
     4141 cgtactcacc gctcacctga tacgcgccaa tcggcaattc agtacgttca cgcagctcac
     4201 gcacgatgtc gaggtacgct ccagcaggtt taaccatcag gcagtctgcg ccctgggctt
     4261 catccagcag tgactcacga atcgcctcgc gacggttcat tgggttcatc tgatagcttt
     4321 tgcggtcgcc ttttaatgcg cttcctgcag cttcacggaa cgggccataa aaggaagagg
     4381 cgaacttggt cgaatacgac ataatcgccg tatctttaaa gcctgcagcg tccagcgcct
     4441 gacgaatcgc ctgtacctgg ccgtccatcg cggcggaagg ggcgatgaag tctgcgcctg
     4501 cagcagctgc aaccacggct tgcttgccta aattttccag agtcgcgtcg ttgtcgacgc
     4561 catgctcgca cagcacaccg cagtgaccgt gagaagtgta ttcacagaaa caagtgtctg
     4621 acatgacgat catttctggc acagtctgct tgcagatgcg cgacatacgc gccaccagtc
     4681 catcttcccg ccaggcgtcg ctgccggttt catcggtatg gtgagagatg ccaaaagtca
     4741 ttacggaacg gataccggcg ttggcgatgc gttcaatttc gcgtgccaga tgtttctctg
     4801 gaatgcgcat cacgcctggc atggcttcaa cggctttgta gtcgtcaatt tcttcttcaa
     4861 caaagatcgg caacaccagg tcgttaaggc taagtgttgt ctcttcaaac atagcgcgca
     4921 gcgcaggaga tttgcgcagg cgacgagggc gttggattaa gtctgtcatg gtctgcctga
     4981 tgtttgtgga attaaggtgc acagtatacc tgaagcgggg caggagtgtt ttacgaaagt
     5041 tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag gacatctttg taatagaaat
     5101 agcaataatt tcatattcac tgaaaatatt tttaatcttt atttatagct cttggttatt
     5161 attttttggc gtttggttgt gctgctataa attgaataat tcaaactttg ttgtatattg
     5221 ataagatatt tatctggttt gtatttttga tatttataac gcgtatgcat caaaaataac
     5281 attatctgca acatactgaa atatcgtcct tttatcaatt tgatgcgtgt ttttctccat
     5341 aaattatata tgtccacatt cggacttagg ggaaagaata attgaaccat tcgtctgtaa
     5401 cgcagcataa tcgttagcgc gaaacataat atgttttcta tgcattgata attgatggat
     5461 caacttatta cgtccctgag gagggatgac aaatgcactc ctggaaaaag aaacttgtag
     5521 tatcacaatt agcattggct tgcactctgg ctatcacctc tcaggctaat gcagcgacca
     5581 acgatatttc tggtcaaact tacaatactt tccatcacta caacgacgcc acctatgctg
     5641 atgacgttta ctatgatggt tatgtaggct ggaacaacta tgccgctgat agctattaca
     5701 acggcgatat ctacccggtc attaataacg ctaccgttaa cggcgtgatt tctacctact
     5761 atctggacga cggtatttct accaatacca acgccaatag tctgacaatc aaaaacagca
     5821 ctattcacgg tatgatttat tccgagtgca tgacaactga ttagactggc cccctgaatc
     5881 tccagacaa
//
LOCUS       DAETQL010000168         5744 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.171, whole genome shotgun sequence.
ACCESSION   DAETQL010000168 DAETQL010000000
VERSION     DAETQL010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.171"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..173
                     /locus_tag="K0W65_003977"
                     /pseudo
     CDS             1..173
                     /locus_tag="K0W65_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            698..1876
                     /gene="ltrA"
                     /locus_tag="K0W65_003978"
     CDS             698..1876
                     /gene="ltrA"
                     /locus_tag="K0W65_003978"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBI3097032.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1959..2138
                     /locus_tag="K0W65_003979"
                     /pseudo
     CDS             1959..2138
                     /locus_tag="K0W65_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2168..2650)
                     /locus_tag="K0W65_003980"
                     /pseudo
     CDS             complement(2168..2650)
                     /locus_tag="K0W65_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2906..3886)
                     /locus_tag="K0W65_003981"
     CDS             complement(2906..3886)
                     /locus_tag="K0W65_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBI3097033.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3886..5085)
                     /locus_tag="K0W65_003982"
     CDS             complement(3886..5085)
                     /locus_tag="K0W65_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBI3097034.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5407..>5744)
                     /locus_tag="K0W65_003983"
     CDS             complement(5407..>5744)
                     /locus_tag="K0W65_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3097035.1"
                     /translation="HWLWYAYNTKTGGVLAYTFGPRNDETCRELLALLTPFCIGMVTS
                     DDWGSYAREVPKEKHLTGKIFTQRIARNNRTLRTRIKRLARKTICFSRSVEIHEKVIG
                     SFIEKHMFY"
BASE COUNT         1574 a         1317 c         1368 g         1485 t
ORIGIN      
        1 ttgctgaact gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta
       61 aagtccatgc cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga
      121 ctgcccggct gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat
      181 aagttatcca tgctctgagg tgggtagagg tgggccctcc ggtcagggtt tacaggagca
      241 cagaccaaac gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa
      301 aaggcagaga tctactgtca cgtgggttcg ggaagctgaa cgaaagtaaa ctgtccgatg
      361 aagcatcgaa atcttagatg atgccaaaat ggggccgtac agccgtcccc tgataagcgc
      421 agaagacacc tgcttactga ctgcgcggca tccggtgtga aggcagcagg atcccgggcc
      481 agaccatctc agggaacgtg agaacctgtt tccggcacgt atcgggaaac aggggcggac
      541 ccattcgtag tagtgatgaa agatctgtaa tgggtctgga gcgaagggat ggggatcaca
      601 ttcattgcag aagacacaac ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt
      661 gaaataccaa aagcactggt ctgggcatca tatctggatg tgcgtcgcaa caagggagcc
      721 ccccggatgc gacgggcaga ctttgaaaat gttcgaccag caacgtgatg gcaatctata
      781 caagatctgg aaccggctat gttcgggaac atggtttccg ccaccagtgc tggaaaaacg
      841 gatcccgaag tcgaatggca aggaacgcat tctggggatc ccaacagtat ccgatcgaat
      901 tgctcaggga gcgataaaac ttttcatgga agaaaagctt gacccgattt tccatgctga
      961 ttcatatggt taccgaccag gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg
     1021 gtgctggcgt tatagctgga tactggaagt ggacatcagt gcgtttttcg accatgtgag
     1081 acatgatctg gtgctcaaag ctctggaaca ccacgggatg cctaaatggg tcatcctgta
     1141 ctgtcgacgc tggatggaag cgccaatgca gagttgtgaa aatggagaat taataacccg
     1201 aacacgaggc actccgcagg gcggggtcat tagtccgcta cttgctaact tgttccttca
     1261 ctatgcattt gatttgtgga tggaaagaga atatcggggg gtaccgtttg agaggtacgc
     1321 tgacgatatt gtagtacatt gttcacgaat gagtgatgca acaaggctaa agaacaggtt
     1381 atcggagcgc ttctcggaag tcgggctggt cctgaatgca gggaaaacga atatcgccta
     1441 cattgacacg tttaaaagga gaaacgtcgc aacgagtttc acctttctcg gatatgactt
     1501 caaagtgcgt acgctgaaga atttcaacgg cgaacggtac cgaaaatgca tgccgggtgc
     1561 gtcaaatgca gcaatgcgca aaataacaga aacaatcaag aagtggcgta tacatcgctc
     1621 aacagctgag agtttgctgg attttgcgag gcgctacaat gcgatagtga gaggctggat
     1681 cgagtactac ggaaagttct ggtccagaaa tttcaactat cgactgtgga gtgcaatgca
     1741 gtcacgtctg ctcaagtgga tgcagtctaa atacagactt tcgaaccgga aggctcagcg
     1801 aaagctgacg ctggtaagga aggagtatcc gaagctattt gttcactggt atttactacg
     1861 tgcatcaaat gagtgatcaa gagccgtatg acgcgagagt gtcacgtacg gttccgtgag
     1921 cagccgggga gtgaaattct ctccggctga ctcgactcgc gggttccgac agcgatgacg
     1981 aacatgcggc ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggaaccag
     2041 aaaaatggct gcgttacgtc attgagcata tccaggactg gccggcaaac cgggtacgtg
     2101 atctgttgcc ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag
     2161 ccgcttactt ttggcgtaat tctgctgcgc ctttactgaa cacaatgccg tattcagcaa
     2221 gaagtccacg aatgtgattt atcagttgtg tcctttccat tgaaaccaac cgacgcatat
     2281 tcaaaaaagt ctggacgtcc tgttgctcca gagtttttat tggtacgggt cgtatagttc
     2341 catgttggca ggcctcagct attgcctgtg cgtcgttata atcatttttc tggcaccgct
     2401 gataagcttt aacatgttgt gccggaagca atattacatc atggtcgagt ttcctgattt
     2461 cccgggccca gtaatgagat gccccgccag cctccatcgc aacagtcatt ttaggctgtt
     2521 gcaccagcca gttaagcagt tgattgcgag aaagtttggt ctttcctgcg ggtttgccat
     2581 gagcattgat actgaaaaga taaaagttcg ttttagccag atcaatgccg gtgattttct
     2641 gttcagacat ggtatcccgc ttacttttgt tgttaaagtt tatccatcat ggcgcaattg
     2701 aaggctgccg tgaaagaggg ggaagactat ctcatcggtt actgttaaac ggctaagaga
     2761 aactatcttt ttcaagcaaa gaagatacgg tgaaacaggc gatttcaccg tgaaatactg
     2821 ttcaaaaaaa acgctaataa aagcaaaatt tcttttaaaa cagccaatta aaaactggcg
     2881 agttgctggc gaaaaacttg aattttcaca gcgaattcag attatctttt agtacaagag
     2941 caatcatacg atctaactta tcctgaactt ccaacggtaa tctgccgaat tcatatgaga
     3001 acgtacgtcc ctttactcgt tttcgagcaa atttatcttt actatcaaat ttccataaca
     3061 aagttacaac cgctttatcc tttactcctg aatcaagcaa tgaagacatt tccttggtta
     3121 taagccgcat aatactgttt ttaacctcgt cttcagaaag attgtcatca gcagtaataa
     3181 gctcaatact cggcgagata ttcttcacaa cttcatcaat agatatatca cccagcctca
     3241 gtcgttcact taattcagcc agttgcctgt aatcggcgta tgtcagctca gactgaacag
     3301 gaaaaagaga cacaaaatct ttaggtacgg acgctgcttg gagggcacga gtcaccttag
     3361 ctgccgacaa tccctctctt tccgcaattt gcttctggtt cataccggcc tctttgagac
     3421 gtactaaacg caacccaatt tcacgaatat tatgttcaag tgacgtctgc aaatctttcg
     3481 ccagatgctg agcttcagaa acagtaagct catcatccgt tacaagtaca cgtaatccaa
     3541 ccttgcatag caaagctgct gcccgacgac gggaaccatc caaaatctca ataagatcgc
     3601 cagtccgaat cccaatacat ggatagaatt gttgtaaacg tatagttctg gtgatgtctt
     3661 ttagtgagtc cggcgtcagg gctgtctgtt cgcggccatt cacctcttca accacaaaag
     3721 tttgagtatc cactttgtca tgatcaacct tgatctcagt aaattttgcc tttcggccag
     3781 tattgagggt aaatacatga actggagccg atatttcttc agtattattc aggatatttg
     3841 tattcagcgt acggccaata gtcggtcggt gctttctgtt ttccatcagt agttggccct
     3901 tataaattca atacgatcaa aaactgcttt agcgaaatct tctgctgcca tacgtgcatt
     3961 tttcaaagct tcaccactgc caacataagt aacaggattc gcagatatca cggtatcaaa
     4021 cgactcgccg cttctctcaa aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc
     4081 accgaaaatc tcttttgtca ggctatggca gtatttatga tctgacttat tcgccagttt
     4141 cgacatgaag ccaatatttg cctgcaaacg gcatgaacat ccatcctgtt caataatctg
     4201 tacaagttca ggtaaacgag ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg
     4261 cggcaccggt gtaaacatga tatctgcggc agcaatggca tttttcaaaa atgcgtccag
     4321 atgcgggcct gtatcaataa ggatgaagtc gaaatcatgc ttcagcttat caataatatt
     4381 ctcacgcaat atcgcgtgtt tgttttgtcc taatagatgc tcctcgcaca gagtatccca
     4441 gtttgatgca ataaaggcgt cgtcaataga agccggcata acataaacgc cagggataac
     4501 tgacggaacg ataaaatctt ccagtaattc ctcacgactc acgttctgca acatagcctg
     4561 cggtgcggtt gtatcaacca gccccaccgc atgcaagtag ttcagaaaca tagtggctga
     4621 tgactgggga tctaaatcaa ggagcagaat acgtaaatct tctgacagca ggtgaggatg
     4681 agcacgcagg gcgtgagcta cggaaacact actcacggtt ttagacacac cgccttttag
     4741 actaccgatg aatatggaat atgcctcact ataacgatcg cgatatttag gaatgccacg
     4801 atgtgcatag atatcgataa tgttctggat ggttaatgca tattttgtcg cggttcctgc
     4861 tgggcgtttt tcaaactgat agccctgagc ctccatctca ccgactgcac catccacgct
     4921 tgcgcgcgtt aattttggaa gcttagctac agcagacttt gaatagactt gataaaaaac
     4981 atcagcctga aactcctgct tttgctcctg aatctgtttt gttagggcga gcaacatttt
     5041 atctgcacgc tgcgcaacct tacttaactg ctcaaaacta gtcattcccc ctccgtgcta
     5101 tgtaggattt taacttatta caaaaaaaac tgcattagca gggtaatgca tgcaagtaca
     5161 tattgtacat gcttatatta aaaatgcgcg cattgctgat ttattaatca tcggaatgaa
     5221 tattactgta agatagtatg cctttccggg agaaagacgc tgatttcccc cgaataatcc
     5281 agttcaccac cacactccgg acaacagctt tcttcaggct ccagatggtg tatttcgcgg
     5341 gaaacatgtt ccggtaacgg acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc
     5401 ttccaatcag tagaacatgt gcttttcaat gaaggagccg atgacttttt cgtggatttc
     5461 cacggaacgc gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt
     5521 ccggttgtta cgtgcaatac gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg
     5581 cacttctctg gcgtagctgc cccagtcatc gctggtcacc atgccgatac agaacggagt
     5641 caacagggct aacagttccc gacaggtctc atcattccgt ggcccgaaag tgtaagccaa
     5701 cacaccgcca gttttagtgt tgtacgcata ccagagccag tgtt
//
LOCUS       DAETQL010000169         5738 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.172, whole genome shotgun sequence.
ACCESSION   DAETQL010000169 DAETQL010000000
VERSION     DAETQL010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.172"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..241
                     /locus_tag="K0W65_003984"
     CDS             <1..241
                     /locus_tag="K0W65_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097036.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="K0W65_003985"
     CDS             complement(238..1179)
                     /locus_tag="K0W65_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HBI3097037.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="K0W65_003986"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="K0W65_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="K0W65_003987"
     CDS             complement(1684..2904)
                     /locus_tag="K0W65_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HBI3097038.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="K0W65_003988"
     CDS             complement(2908..3651)
                     /locus_tag="K0W65_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBI3097039.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="K0W65_003989"
     CDS             complement(3542..5008)
                     /locus_tag="K0W65_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HBI3097040.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5241)
                     /locus_tag="K0W65_003990"
     CDS             complement(5008..5241)
                     /locus_tag="K0W65_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HBI3097041.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5229..5708)
                     /locus_tag="K0W65_003991"
     CDS             complement(5229..5708)
                     /locus_tag="K0W65_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097042.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1225 a         1618 c         1436 g         1459 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccgggacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DAETQL010000170         5702 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.173, whole genome shotgun sequence.
ACCESSION   DAETQL010000170 DAETQL010000000
VERSION     DAETQL010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.173"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..170)
                     /locus_tag="K0W65_003992"
     CDS             complement(<1..170)
                     /locus_tag="K0W65_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097043.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="K0W65_003993"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="K0W65_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBI3097044.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="K0W65_003994"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="K0W65_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /locus_tag="K0W65_003995"
     CDS             complement(3616..4383)
                     /locus_tag="K0W65_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBI3097045.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5487
                     /locus_tag="K0W65_003996"
                     /pseudo
     CDS             4579..5487
                     /locus_tag="K0W65_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            5484..>5702
                     /locus_tag="K0W65_003997"
     CDS             5484..>5702
                     /locus_tag="K0W65_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HBI3097046.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQ"
BASE COUNT         1410 a         1469 c         1481 g         1342 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attcagccgt cgatattttt gttaaggatc ttggtctggt tgccgataca
     5341 gccaaagccc tgcacttccc gctgccattg gcctcaacag cattgaatat gttcaccagc
     5401 gccagtaacg cgggttacgg gaaagaagac gatagcgcag ttatcaagat tttctctggc
     5461 atcactctac cgggagcgaa atcatgatca agattggcgt tatcgccgat gattttaccg
     5521 gcgcgacgga tatcgccagt tttctggtgg aaaacggtct accaacggta caaattaacg
     5581 gtgttccaac aggtaaaatg ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc
     5641 gctcctgtcc ggtggttgaa gccacacagc aatcgctggc ggctctgagc tggttgcaac
     5701 ag
//
LOCUS       DAETQL010000171         5638 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.175, whole genome shotgun sequence.
ACCESSION   DAETQL010000171 DAETQL010000000
VERSION     DAETQL010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.175"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            84..299
                     /locus_tag="K0W65_003998"
     CDS             84..299
                     /locus_tag="K0W65_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBI3097047.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            292..678
                     /locus_tag="K0W65_003999"
     CDS             292..678
                     /locus_tag="K0W65_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HBI3097048.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            686..1372
                     /locus_tag="K0W65_004000"
     CDS             686..1372
                     /locus_tag="K0W65_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBI3097049.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1350..1973)
                     /gene="tetR(B)"
                     /locus_tag="K0W65_004001"
     CDS             complement(1350..1973)
                     /gene="tetR(B)"
                     /locus_tag="K0W65_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HBI3097050.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2055..3260
                     /gene="tet(B)"
                     /locus_tag="K0W65_004002"
     CDS             2055..3260
                     /gene="tet(B)"
                     /locus_tag="K0W65_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HBI3097051.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3373..3966)
                     /gene="tetC"
                     /locus_tag="K0W65_004003"
     CDS             complement(3373..3966)
                     /gene="tetC"
                     /locus_tag="K0W65_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HBI3097052.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4099..4470
                     /locus_tag="K0W65_004004"
     CDS             4099..4470
                     /locus_tag="K0W65_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3097053.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4480..>5638)
                     /locus_tag="K0W65_004005"
     CDS             complement(4480..>5638)
                     /locus_tag="K0W65_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HBI3097054.1"
                     /translation="LHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKRI
                     DRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHGR
                     SVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLGW
                     YWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKSR
                     SKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIEE
                     TFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQAN
                     TVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1562 a         1041 c         1225 g         1810 t
ORIGIN      
        1 gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat taccgaccac
       61 tattaagtca tgtagctggt tttatttcaa ttgaacgttt tcaaagtcta gctacagaag
      121 gaaaaatgtt atcgctatct tggtgggaaa acgaatacgc agttctgcaa tggaaaaatc
      181 atgttttaca tgcgaaagct caacaagaag ggcgagagtc aatatttgat ttttacaaaa
      241 ttagtattgc tcatattact cgcgaatatt catttaaaaa ggacaaggat aatgtttgat
      301 gttcacgttg ttttagataa tcaaatagga caattagcat tactaggaaa aacattaggt
      361 aataaaggta ttggattgga agggggaggg atatttacgg ttggtgatga atgccatgct
      421 cattttcttg ttgaacaagg aaaggaagct aaaatagcgc tagagcaagc tggactgtta
      481 gtacttgcga tccggacacc attaattcgt aagttaaaac aggaaaaacc gggggaactt
      541 ggcgaaatag cacgagtatt ggcggagaat aacattaata ttttagtgca atacagtgac
      601 catgctaacc aactgatatt aataacggac aatgatagta tggctgcatc tgttacgctc
      661 ccttgggcaa taaagtgaac ttgcgatggc taatttaata cgaaaagagg ttacctttga
      721 gtcctcaata gccgcgatag gggcggctat gtctgacatt tcacgagtta aaatactcag
      781 tgctttgatg gatgggcgag cttggacggc cactgagcta agttctgtgg cgaatatatc
      841 agcttcaacg gcgagcagtc atttatctaa attattagat tgccagctaa tcacagtagt
      901 agctcaaggc aagcatcgtt attttcggct agcaggaaaa gatattgctg aattgatgga
      961 aagtatgatg gggatctcct taaaccatgg cgtacatgcc aaagtttcca cgccagtgca
     1021 tttacgaaaa gcacgtactt gctatgatca tttagctggc gaagttgccg ttaagatcta
     1081 tgattccctt tgtcaacagc aatggatcac tgaaaatggt tcaatgatca cattaagtgg
     1141 tattcaatat tttcatgaaa tgggaattga cgttccttcc aaacattcac gtaaaatctg
     1201 ttgtgcgtgt ttagattgga gtgaacgccg tttccattta ggtgggtacg ttggagccgc
     1261 attattttcg ctttatgaat ctaaagggtg gttaactcga catcttggtt accgtgaagt
     1321 taccatcacg gaaaaaggtt atgctgcttt taagacccac tttcacattt aagttgtttt
     1381 tctaatccgc atatgatcaa ttcaaggccg aataagaagg ctggctctgc accttggtga
     1441 tcaaataatt cgatagcttg tcgtaataat ggcggcatac tatcagtagt aggtgtttcc
     1501 ctttcttctt tagcgacttg atgctcttga tcttccaata cgcaacctaa agtaaaatgc
     1561 cccacagcgc tgagtgcata taatgcattc tctagtgaaa aaccttgttg gcataaaaag
     1621 gctaattgat tttcgagagt ttcatactgt ttttctgtag gccgtgtacc taaatgtact
     1681 tttgctccat cgcgatgact tagtaaagca catctaaaac ttttagcgtt attacgtaaa
     1741 aaatcttgcc agctttcccc ttctaaaggg caaaagtgag tatggtgcct atctaacatc
     1801 tcaatggcta aggcgtcgag caaagcccgc ttatttttta catgccaata caatgtaggc
     1861 tgctctacac ctagcttctg ggcgagttta cgggttgtta aaccttcgat tccgacctca
     1921 ttaagcagct ctaatgcgct gttaatcact ttacttttat ctaatctaga catcattaat
     1981 tcctaatttt tgttgacact ctatcattga tagagttatt ttaccactcc ctatcagtga
     2041 tagagaaaag tgaaatgaat agttcgacaa agatcgcatt ggtaattacg ttactcgatg
     2101 ccatggggat tggccttatc atgccagtct tgccaacgtt attacgtgaa tttattgctt
     2161 cggaagatat cgctaaccac tttggcgtat tgcttgcact ttatgcgtta atgcaggtta
     2221 tctttgctcc ttggcttgga aaaatgtctg accgatttgg tcggcgccca gtgctgttgt
     2281 tgtcattaat aggcgcatcg ctggattact tattgctggc tttttcaagt gcgctttgga
     2341 tgctgtattt aggccgtttg ctttcaggga tcacaggagc tactggggct gtcgcggcat
     2401 cggtcattgc cgataccacc tcagcttctc aacgcgtgaa gtggttcggt tggttagggg
     2461 caagttttgg gcttggttta atagcggggc ctattattgg tggttttgca ggagagattt
     2521 caccgcatag tccctttttt atcgctgcgt tgctaaatat tgtcgctttc cttgtggtta
     2581 tgttttggtt ccgtgaaacc aaaaatacac gtgataatac agataccgaa gtaggggttg
     2641 agacgcaatc gaattcggta tacatcactt tatttaaaac gatgcccatt ttgttgatta
     2701 tttatttttc agcgcaattg ataggccaaa ttcccgcaac ggtgtgggtg ctatttaccg
     2761 aaaatcgttt tggatggaat agcatgatgg ttggcttttc attagcgggt cttggtcttt
     2821 tacactcagt attccaagcc tttgtggcag gaagaatagc cactaaatgg ggcgaaaaaa
     2881 cggcagtact gctcggattt attgcagata gtagtgcatt tgccttttta gcgtttatat
     2941 ctgaaggttg gttagttttc cctgttttaa ttttattggc tggtggtggg atcgctttac
     3001 ctgcattaca gggagtgatg tctatccaaa caaagagtca tcagcaaggt gctttacagg
     3061 gattattggt gagccttacc aatgcaaccg gtgttattgg cccattactg tttgctgtta
     3121 tttataatca ttcactacca atttgggatg gctggatttg gattattggt ttagcgtttt
     3181 actgtattat tatcctgcta tcgatgacct tcatgttaac ccctcaagct caggggagta
     3241 aacaggagac aagtgcttag ttatttcgtc accaaatgat gttattccgc gaaatataat
     3301 gaccctcttg ataacccaag agggcatttt ttacgataaa gaagatttag cttcaaataa
     3361 aacctatcta ttttatttat ctttcaagct caataaaaag ccgcggtaaa tagcaataaa
     3421 ttggcctttt ttatcggcaa gctcttttag gtttttcgca tgtattgcga tatgcataaa
     3481 ccagccattg agtaagtttt taagcacatc atcatcataa gctttaagtt ggttctcttg
     3541 gatcaatttg ctgacaatgg cgtttacctt accagtaatg tattcaaggc taattttttc
     3601 aagttcattc caaccaatga taggcatcac ttcttggata gggataaggt ttttattatt
     3661 atcaataata taatcaagat aatgttcaaa tatactttct aaggcagacc aaccatttgt
     3721 taaatcagtt tttgttgtga tgtaggcatc aatcataatt aattgctgct tataacaggc
     3781 actgagtaat tgttttttat ttttaaagtg atgataaaag gcacctttgg tcaccaacgc
     3841 ttttcccgag atctcatcta ttgaaacagc ttgatagcct ttttcaacaa acaatattcg
     3901 tgctgagtta accagtgatt gataggtact cttaaaattt tcttgttgat gatttttatt
     3961 ttccatgata gatttaaaat aacataccgt cagtatgttt atggtatcat gatgatgtgg
     4021 tcgtgacaat cttaagaaca tttaggttat tttatgtata ttgaacagca ttctcgctat
     4081 caaaataaag ctaataacat ccaattaaga tatgatgata agcagtttca tacaacggtt
     4141 atcaaagatg ttctattatg gattgaacat aatttagatc agtctttact gcttgatgat
     4201 gtggcgaata aagcgggtta taccaagtgg tattttcagc ggctgttcaa aaaagtaaca
     4261 ggggtcacac tggctagcta tattcgtgct cgtcgtttga cgaaagcggc tgttgagttg
     4321 aggttgacga aaaaaactat ccttgagatc gcattaaaat atcaatttga ttcccaacaa
     4381 tcttttacac gtcgatttaa gtacattttt aaggttacac caagttatta tcggcgtaat
     4441 aaattatggg aattggaggc aatgcactga gagatcccct cataatttcc ccaaagcgta
     4501 accatgtgtg aataaatttt gagctagtag ggttgcagcc acgagtaagt cttcccttgt
     4561 tattgtgtag ccagaatgcc gcaaaacttc catgcctaag cgaactgttg agagtacgtt
     4621 tcgatttctg actgtgttag cctggaagtg cttgtcccaa ccttgtttct gagcatgaac
     4681 gcccgcaagc caacatgtta gttgaagcat cagggcgatt agcagcatga tatcaaaacg
     4741 ctctgagctg ctcgttcggc tatggcgtag gcctagtccg taggcaggac ttttcaagtc
     4801 tcggaaggtt tcttcaatct gcattcgctt cgaatagata ttaacaagtt gtttgggtgt
     4861 tcgaatttca acaggtaagt tagttgctag aacccatggc tcctttgccg acgctgagta
     4921 gattttaggt gacgggtggt gacaatgagt ccgtgtcgag cgctgatttt ttcggccttt
     4981 agagcgagat ttatacaata gaatttggca tgagattgga ttgcttttag tcagcctctt
     5041 atagcctaaa gtctttgagt gactagatga catatcatgt aagttgctga taggtttcca
     5101 gttttccgct cctaggtctg catattgtac ttttcctctt actcgactta accagtacca
     5161 acccagcttc tcaacggatt tataccatgg cactttaaag ccagcatcac tgacaatgag
     5221 cggtgtggtg ttactcggta gaatgctcgc aaggtcggct agaaattggt catgagcttt
     5281 ctttgaacat tgctctgaaa gcgggaacgc tttctcataa agagtaacag aacgaccgtg
     5341 tagtgcgact gaagctcgca ataccataag tcgtttttgc tcacgaatat cagaccagtc
     5401 aacaagtaca atgggcatcg tattgcccga acagataaag ctagcatgcc aacggtatac
     5461 agcgagtcgc tctttgtgga ggtgacgatt acctaacaat cggtcgattc gtttgatgtt
     5521 atgttttgtt ctcgctttgg ttggcaggtt acggccaagt tcggtaagag tgagagtttt
     5581 acagtcaagt aatgcgtggc aagccaacgt taagctgttg agtcgtttta agtgtaat
//
LOCUS       DAETQL010000172         5599 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.176, whole genome shotgun sequence.
ACCESSION   DAETQL010000172 DAETQL010000000
VERSION     DAETQL010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.176"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..460
                     /locus_tag="K0W65_004006"
                     /pseudo
     CDS             1..460
                     /locus_tag="K0W65_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            625..4743
                     /gene="pic"
                     /locus_tag="K0W65_004007"
     CDS             625..4743
                     /gene="pic"
                     /locus_tag="K0W65_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HBI3097055.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4894..5136)
                     /locus_tag="K0W65_004008"
                     /pseudo
     CDS             complement(4894..5136)
                     /locus_tag="K0W65_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5175..>5599)
                     /locus_tag="K0W65_004009"
     CDS             complement(5175..>5599)
                     /locus_tag="K0W65_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3097056.1"
                     /translation="EQRLAARKARAAPLMQSLYDRIQQQMKTLSRHSDTAKAFAYLLK
                     QWKALNVYCSNGWVEIDNNIAENALRAVSLGRKNFLFFGSDHGGERGALLYSLIGTCK
                     LNDVDPESYLRHVLGVIADWPVNRVSELLPWRIALPAE"
BASE COUNT         1464 a         1297 c         1498 g         1340 t
ORIGIN      
        1 gggcttcgtc tatgtggcgt tcatcattga tgtgtttgcc ggatacatcg tgtggtgacg
       61 agtctcatcg tctatggaaa cgacattcgt gctggatgca ctggagcagg cgttgtgggc
      121 ccgtcgtccg tctggcacca tccatcacag cgataaaggc tctcagtatg tgtcactggc
      181 ctataaggcg cgactaaaag aagcaaaact gctggcatca acagggagta ctggtgactc
      241 gtatgacaac gcgatggcag agatcatcaa gggcctttac aaagcggagg taatacaccg
      301 taagagctgg aaaaaccgga cagaagtgga gctggccaca ctcacgtggg tggactggta
      361 taacaatcga cgattgctgg aaaggctggg ccatatccca ccggcagaag cagaaaaagc
      421 ttattatgct tccatcggaa atgatgatct ggcagcctga gttcacagat aaaacactct
      481 ccaggaaacc cggggcggtt cagttcacaa aaacacatta atgcagtaac tatattttcc
      541 tttctggtga taacgtcggg ttatcattag cttcttcagc tattttactt ttatatccct
      601 tgtaaacatc atggagaatc catagtgaat aaagtttatt ctcttaaata ttgccccgtc
      661 accggggggc ttattgctgt ctctgaactt gcccgcaggg taataaaaaa gacatgccga
      721 agattaacgc atattcttct ggctggcatt ccagcaatct gtctgtgtta ctctcagata
      781 tcccaggcgg gtattgtccg ttccgatatt gcctatcaga tttatcgtga tttcgccgaa
      841 aacaaagggc tttttgtacc tggtgccaat gatattccgg tatatgataa ggacggaaaa
      901 cttgtgggaa gactgggtaa agccccaatg gccgatttca gcagtgtgag ctcaaatggc
      961 gttgctacgc ttgtatcgcc tcagtatatc gtcagcgtaa agcataacgg aggatatcgg
     1021 agtgtgagct ttggtaatgg gaaaaataca tattcccttg ttgaccgtaa taaccaccct
     1081 tctattgact tccatgctcc acgtctgaat aaactggtta cagaagttat tccctcagcg
     1141 gtaacatcag aaggaaccaa agccaatgct tataaataca ctgaacgtta caccgctttt
     1201 tatcgggtgg gtagtggtac gcagtacact aaggacaagg acggaaattt agttaaggtt
     1261 gccggtggat atgcttttaa aacaggagga accacaggag ttcctctgat atctgatgca
     1321 acaatagtct ctaatcccgg gcaaacttat aatcctgtaa acggcccttt acctgactat
     1381 ggagcccctg gggatagtgg ttctcctttg tttgcttatg ataaacaaca aaaaaaatgg
     1441 gttattgttg ctgtattaag agcatatgca ggtattaatg gtgctacgaa ctggtggaat
     1501 gtcataccaa cagattatct gaaccaggtt atgcaggacg atttcgatgc ccccgtagac
     1561 tttgtttccg gactgggccc cctgaactgg acatacgaca aaacatcagg cacaggtacc
     1621 ctgagccagg gcagtaaaaa ctggaccatg cacgggcaga aagacaatga cctcaatgcc
     1681 ggtaaaaatc tggtattcag cgggcagaat ggtgcaatta tcctgaaaga cagtgtgact
     1741 cagggtgccg gttatctcga atttaaagac agttacaccg tatctgctga atccggaaaa
     1801 acatggacgg gtgccggcat tattactgac aaggggacga atgtaacctg gaaggtcaac
     1861 ggcgttgccg gtgacaactt gcataagctg ggggaaggaa ccctgaccat aaacggaaca
     1921 ggtgtaaacc cgggaggact gaaaacggga gacggtatcg ttgtacttaa ccagcaggca
     1981 gacactgcag gtaatatcca ggccttcagt tcagtgaacc tcgccagcgg acgtccgacc
     2041 gtggtgctcg gggatgcccg tcaggtcaat ccggataaca tttcatgggg ataccgggga
     2101 ggtaagcttg accttaatgg taatgccgtt accttcaccc gactgcaggc tgctgattac
     2161 ggggcggtga ttacaaataa tgcacagcaa aaatcccagc ttttactgga tcttaaggct
     2221 caggatacaa atgtcagtga accgacgatt ggaaatatat ccccctttgg tggtaccggc
     2281 actccaggaa acctgtacag catgatactc aacagccaga cccgcttcta tattctgaaa
     2341 tctgccagct atggtaacac tctgtggggg aacagcctga atgatccggc tcagtgggag
     2401 tttgttggca tgaacaaaaa caaagcagtt cagacagtaa aagataggat cctggccggg
     2461 cgggcaaaac aacccgttat ctttcatggt cagctgaccg ggaatatgga tgtcgccatt
     2521 ccacaggtgc cggggggaag aaaggtaatc tttgatggta gcgtgaacct gccggaaggt
     2581 accctgagtc aggacagtgg caccctgata ttccagggac atccggttat ccatgcctcc
     2641 atcagtggca gtgcaccggt cagcctgaac cagaaagact gggaaaaccg tcagtttaca
     2701 atgaaaacac tgtcgctgaa agacgctgac ttccatcttt cacgtaacgc ctcgctgaac
     2761 agtgacatta agtcggataa cagccatatc acactgggaa gtgacagggc atttgtggat
     2821 aaaaatgacg gaacaggaaa ttatgtcatt ccggaggaag gtacctctgt cccggacacc
     2881 gtgaatgaca ggagccagta tgaagggaat attacgctga accataactc agccctggat
     2941 atcggcagca ggttcaccgg ggggattgac gcttatgaca gtgccgtcag catcacctct
     3001 ccggacgtcc tgttgacagc cccgggtgct tttgccggca gttcactgac agtgcatgat
     3061 ggcggtcatc ttacagcact gaacggtctt ttcagcgacg ggcatattca ggccggtaag
     3121 aacggcaaaa tcaccctgag cggtacaccg gttaaagata cggctaatca gtatgcccct
     3181 gctgtatatc tgacggacgg atatgacctg accggcgata acgcagcact ggaaattacc
     3241 cgtggagcac atgcttccgg tgatattcat gcctctgcgg catcaacagt taccatcggg
     3301 tctgacacgc cggcagaact ggcttctgcg gaaacggctg catcggcgtt tgccggcagt
     3361 cttcttgagg gctataacgc agcattcaat ggtgccataa ccggtggcag ggctgatgtc
     3421 agtatgcata atgcactgtg gactctgggt ggggactctg ccatccacag tcttaccgtc
     3481 agaaacagcc gtattagttc tgaaggagac cgtacattcc gtaccctgac ggtgaataaa
     3541 ctggatgcaa caggcagtga ttttgttttg cgtacggacc tgaaaaatgc cgataaaatt
     3601 aatgtgactg aaaaagccac tggttcagat aacagcctga acgtcagctt tatgaataat
     3661 cctgctcagg gacaggccct gaatattcct ctggtcacgg caccggcggg aacttcagca
     3721 gagatgttta aggccggcac ccgggtgaca ggtttcagtc gggtgacccc aaccctgcat
     3781 gttgatacca gtggtggcaa tacgaagtgg atactggatg gttttaaagc ggaggctgat
     3841 aaagccgctg ccgcgaaggc tgacagtttc atgaatgccg ggtataaaaa cttcatgacg
     3901 gaagttaaca atctgaacaa acgtatgggt gacctgcgtg acacaaacgg tgatgccggt
     3961 gcctgggcgc gcatcatgag tggtgccggt tctgcagacg gtggttacag tgataattac
     4021 acccatgttc aggtcggctt tgacaaaaaa catgaactgg acggtgtgga cctgtttacc
     4081 ggtgtcacga tgacctatac cgacagcagt gcagacagcc atgcattcag cggaaagacg
     4141 aaatcggtgg ggggcggtct gtatgcttca gcattgtttg agtccggtgc ctatatcgat
     4201 ttgattggta aatatattca ccatgacaat gattacacag gtaactttgc tagcctggga
     4261 acgaaacact acaacaccca ttcctggtat gccggtgctg aaacgggtta ccgctatcac
     4321 ctgacagagg acacgttcat tgagccgcag gctgaactgg tttacggcgc cgtgtccggg
     4381 aaaacattcc gctggaaaga cggtgatatg gacctgagca tgaagaacag ggacttcagt
     4441 ccgctggttg gaagaacagg ggttgaactg ggcaagacct tcagtggtaa ggactggagt
     4501 gtgacggccc gtgccggaac cagctggcag tttgacctgc tgaataatgg agagaccgta
     4561 ctgcgtgatg cgtccgggga gaaacggata aaaggagaga aggacagccg gatgctgttt
     4621 aatgttggta tgaatgcgca gataaaggac aatatgcgct ttggtctgga gtttgagaag
     4681 tcagcctttg gtaaatataa cgtggataat gcggtaaacg cgaatttccg gtatatgttc
     4741 tgatgtgggg gtaaagggct gccggaaggc agcctttctc agagactctt aaaatataaa
     4801 actgaaggtc ttcggtaagg gggagtgtat tagacgtcaa ctagttttgc cgactggcgc
     4861 gagcctgttt aagattctgt gtaaatgcct ttctcagaag tgaccgtcca ggcggtcacc
     4921 gaactcgata ataaagcggc tcattgccat tcgccagtcc ttcaacggca tcgtccattt
     4981 ctgggacgca gactggattg ccagccacac cacttttttc accgagtcgt ctgtcgggaa
     5041 cactttacgc tttttgatcg catggcggat cacgctgttt agcgactcga tggcattcgt
     5101 cgtatagatc actttgcgga tgtccgttgg ataagcggta agcgtacagc gagggccgta
     5161 ttgacgggga tgtgttattc agctggcagt gctatgcgcc acggaagcag ttcgctgacc
     5221 cggttgaccg gccagtctgc tatgacgcca agcacatggc gaaggtagct ttctggatcc
     5281 acgtcattca gtttgcacgt cccgatcagg ctgtacagta gcgcgccccg ctcaccacca
     5341 tggtcagagc cgaagaacag gaagttttta cgacccagac tgaccgcccg cagggcattt
     5401 tcagcgatgt tgttgtcgat ttccacccag ccattactgc agtacacgtt cagggccttc
     5461 cactgtttca gtaggtatgc gaacgctttt gccgtatccg agtgacgcga cagtgttttc
     5521 atctgttgct gtatccggtc atacagtgac tgcatcagtg gcgcggctct tgcttttctt
     5581 gccgccagac gctgttctg
//
LOCUS       DAETQL010000173         5592 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.177, whole genome shotgun sequence.
ACCESSION   DAETQL010000173 DAETQL010000000
VERSION     DAETQL010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.177"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..279)
                     /locus_tag="K0W65_004010"
     CDS             complement(1..279)
                     /locus_tag="K0W65_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097057.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="K0W65_004011"
     CDS             220..405
                     /locus_tag="K0W65_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097058.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="K0W65_004012"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="K0W65_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBI3097059.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="K0W65_004013"
     CDS             complement(1254..1670)
                     /locus_tag="K0W65_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097060.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="K0W65_004014"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="K0W65_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2862
                     /locus_tag="K0W65_004015"
     CDS             1837..2862
                     /locus_tag="K0W65_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI3097061.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2915..3004)
                     /locus_tag="K0W65_004016"
     tRNA            complement(2915..3004)
                     /locus_tag="K0W65_004016"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="K0W65_004017"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="K0W65_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBI3097062.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="K0W65_004018"
     tRNA            3996..4071
                     /locus_tag="K0W65_004018"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="K0W65_004019"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="K0W65_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1509 a         1326 c         1293 g         1464 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct at
//
LOCUS       DAETQL010000174         5557 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.178, whole genome shotgun sequence.
ACCESSION   DAETQL010000174 DAETQL010000000
VERSION     DAETQL010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.178"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..641
                     /locus_tag="K0W65_004020"
     CDS             <1..641
                     /locus_tag="K0W65_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HBI3097063.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="K0W65_004021"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="K0W65_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBI3097064.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="K0W65_004022"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="K0W65_004022"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBI3097065.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1376 a         1360 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatcg
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagctatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaa
//
LOCUS       DAETQL010000175         5553 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.179, whole genome shotgun sequence.
ACCESSION   DAETQL010000175 DAETQL010000000
VERSION     DAETQL010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.179"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(107..1816)
                     /locus_tag="K0W65_004023"
     CDS             complement(107..1816)
                     /locus_tag="K0W65_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HBI3097066.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2248..2967)
                     /locus_tag="K0W65_004024"
     CDS             complement(2248..2967)
                     /locus_tag="K0W65_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HBI3097067.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3092..3648)
                     /locus_tag="K0W65_004025"
                     /pseudo
     CDS             complement(3092..3648)
                     /locus_tag="K0W65_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3632..3890
                     /locus_tag="K0W65_004026"
                     /pseudo
     CDS             3632..3890
                     /locus_tag="K0W65_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(3999..4577)
                     /locus_tag="K0W65_004027"
     CDS             complement(3999..4577)
                     /locus_tag="K0W65_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097068.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(4998..5235)
                     /locus_tag="K0W65_004028"
                     /pseudo
     CDS             complement(4998..5235)
                     /locus_tag="K0W65_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(5422..>5553)
                     /locus_tag="K0W65_004029"
     CDS             complement(5422..>5553)
                     /locus_tag="K0W65_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBI3097069.1"
                     /translation="LKVDPLKCELRGGQMRFTGLKRGYRLAEQVMMHEPLARIRWCG"
BASE COUNT         1567 a         1213 c          965 g         1808 t
ORIGIN      
        1 gttgccaatt gaggtggttg cctgaaagtt ggcagttacc cggtcactac accttgttgg
       61 agtgaacata tcaaaaacag gtaattacac aatttaaatt acagtctcat aaaaaatgat
      121 taaatctatt tattatttca ataaacgttt ttttcatctt ctttgaaaat tgttctgcta
      181 tagtatccgg ttccagattg ttctctttta ttattctaca atactctaat attgtttctt
      241 tgtctctatt tactacagat acaaatacac caggaatacc atctttatta gttgcggaca
      301 gcatttcgac caatcgtctt gtggtaaaac tatagttttt tagaatatta caaaaggcac
      361 gtattgtttc ggtatggcca ttataaaatg ccataaaaag tcctgatgta ccgttgggat
      421 tttttatcca taataattca tctatatatt cctggtgagg ggggagtatt tttagtatat
      481 ctccatacgc cataatggcc tttgtgtgcc cattttgaag tgccataaac aacccatgct
      541 ttactttcaa taattcttta atttgttcac tactaagcat tttcttatta agcagacttc
      601 cgaacatacg aatactatct gcatgtccat tctgcaatgc aaaaaataac ccaggtaaat
      661 catggctgtc tttcgcagct aatagatata atctcttatc ggaagttaac ggggttttaa
      721 agactatttc cccataggct tttattgtgt ctacatgtcc gttcataaaa gacatgcata
      781 aacccggcac attgtttgcc cctaccgcag caagcaatgt aagtattgtt tcttgactaa
      841 gattgcacat atttaatatc ttaccatata cttgaataat gtctgaatat ccgttttgaa
      901 gtgctatata aaatgcagga gtaccatcag agcttctcgt ggccagaagg ttcaccagtt
      961 tagattgaga aatttcacca tttttgtatt tttccatcag tataaataat acttctgtca
     1021 gattctttgt tatcgcatga tatataactc tggaattgat caattcatct ggtaaaagtt
     1081 tcactgtgtt tgttagaggg gaattatgcc tatcttcaaa tattacctgc ccgggttgct
     1141 ccagaccata aattttccaa taaaaatcat caaaaaaatc cttagcggat aattgactca
     1201 gttcgaaatg gctatcacaa gtaaatacag tacgtttatg agtaacagtt cgattaggat
     1261 cgaaaaactg aacaacccat ttttcacttt cttgtacgcg ctttattttc aaccctaacg
     1321 ccatttggtg gtttaatgtg catgctgata atgttctgac aaagtctcca ttttttttca
     1381 tgttataaaa aaaattagta ataactgttc cccacttttt attttccaca aaataaatat
     1441 aagctggtga ttttgaaaaa tcttccgttt tttccaaaca tacatttttt gcaatagcat
     1501 caggggtgct aagaagctga tagtttacct tcccatttgt ttccataaaa ttcatttccc
     1561 aataggaagc taggtgacgg caaactattt gtcttccata tatatctttt gccactccat
     1621 ttatatttac ctccacattt ctcttttctg aaaaataagg tgctgtttta ttcttaaact
     1681 gaattagttc actggagttt gagaccctca taatagaact tgttattgtt ctattatctc
     1741 tgttaacaga catatctgtt gataacgaat tttttgttga aaatatacta gatgaaaatg
     1801 ttttatttaa cggcatagaa gacaaccata gagatgttat aatactatta gattgcagtt
     1861 actaatagta aaagcctcct tacaaggagg tagcttattc agaatgctcc tataagatag
     1921 cgagctcgtc aacgatacag atgatctgat tatattgtgc aaaaggagaa caggaaaata
     1981 ggcactggaa aagatgaagg cctgatggga gcattaacta atgcaaaatc cgacaggtta
     2041 atatcccgaa aagcaatcaa acgactcaaa caggagatgc acgctgaaat ggtggacaac
     2101 tgttcggatt tcagatgagt agctcagcag tttatgagcc gaatgctctg tcacgggcag
     2161 gaatcaggct gcatacgcag tgccaaatct aatcgtagtt attctaacca gttttcaagc
     2221 tacaaggtgg tgtagctggc atcttctcta ctctatcatc aaacgataaa atggttcctc
     2281 tcgtaacatt tgctcttgcc tttcacttcc atctcgatcg cttcgtagtt cattacgata
     2341 gctgacatat ttccagtctt caggtctaac atctgacgcc ggatactccc cgggagcgat
     2401 tttgcttctt aatagattag actcgagaca cattataaat tgtcgtattt tatgaagtaa
     2461 taaagcacta tattgcgagc attcctgatc tgattttaca tatagcgtaa actgagctcc
     2521 tatccccaca cgagattgct gagaaactcg attcatatca gttatcttcc atttatctat
     2581 aggactgtcc tctgaaaata ataaaccaga caaaatttgg aatgccagag gaacttgctc
     2641 cctagctata ctaatatgaa acttgtcccc aacaaaatcc cccttactct gaggattttc
     2701 acgacatgca tgaataaaca catctccact atggttcaca atctggaatc cttggtacat
     2761 gacagaaaaa gattgacata tatcagagta agttggcacg ttcgatgcta ctttgaaata
     2821 gtacgctggg tattgccgta tttggttgta caatatattg aagttatttt tcaatctttc
     2881 attcgcagaa agcatctgcg gtatttcaga cctcacaaca ttcaaagaat ctaaatttag
     2941 ttttagacag ggctttttta tgggcataga aaacgtcctc tataaaatag atatattatc
     3001 ttatattgat tgtttatcat cttttataaa acgatacatt atatgtaatg tatgtaagcg
     3061 taagggtcct ctccgaacat ggtgggaagg cttagaacgc catattcagt ttcccatagt
     3121 ggaacatcac tcccagctta aagcgctccc ggttgcggta gcccttggct tttattctca
     3181 gcagcctgat cttgctgttc agtgcttccg catttccgtt tgatacgcca tggcgcatgg
     3241 cgttcaggat accgtacaac cttttcccta tcgttttagc cacattcttc atcatgggga
     3301 cgtcactgtt ggccgcagcc atctctgcca gtcgttgcgt ctttcctcgc tccatggtct
     3361 gctccagata tcctttgcca gttctttcag cgcccagcac aggctcgtca gcttcatctg
     3421 cgcacgcagc catatcagct tttcctgcct ggattcggtc atccatttat cgctgtgctg
     3481 ccacaggaag cgagttccct ttgcctgacg acgactttca acaggaagat gcggatgttc
     3541 attctggcgg gttttatcca ccacctcacc cggttgtttc gccacatgaa agcgatcgaa
     3601 ggctattttc tcaaccgcat tgggtaagtg gatgcgcgct gctcttagtt tgctgagcca
     3661 gtttagcatt gccttctaca tgaccgatgc ctgagccggt ataccgaact ttattgtcct
     3721 caaccagtca tgttataagc aagaaatgca ctcagcggat agagcgacat aatctgaacc
     3781 tgcggacaca tcttaagcgg cttacccgca aaaccatatg cttctcaaag tctgatgata
     3841 tgcactacaa gatcatcggc tggtatctga caatcaacca ccaccactaa atctgcgtca
     3901 cgacctgcaa ctgagtctgg ttgaacaggc tgaggccctg ctgaagcaac gcgaacctct
     3961 cgactcttgt tggattttac caaatagttt ttctaaaatt aaacacattt atataagttc
     4021 tttgctgaac aaaacgcagc ccgccatcct ttttcattca taaattcagc aatggcagat
     4081 aaaacttttt catcaagcat ataaaaaata atgccagtag actcaatatg cctcatacaa
     4141 ataaggccgc acatatccgt aatatatttg tcaatgctgc taccatttgc atggtaatat
     4201 gtcatgctat tatctgatga ttttacatat gcagtatgac atatttttcc ttcacgatta
     4261 acaaaactga caaaaccact ttcaccagga gtaatatata aagatgaatt taacgtgcgc
     4321 tcactaatat tcattaattg ttgaaattca atggaatagt tttcagttaa tgtatctatt
     4381 atcttggctt tgtctttctt tgttatctca ttgtcttttt cggcaagaaa aattgccact
     4441 ttatgacatg cattcagctc gttaatttta cctgataaaa ctgcttttct taccacatcg
     4501 ttgaactcat cgatagtgtg ttttttatta tatatatcat gaaaaatata gcctaaagac
     4561 ttaaacgaga ctttcatttt atattctcct taaatattaa caggatatat atatcacatt
     4621 ttcctctata ttttcagaaa ccagctatca ttttccccat tactggtacg tcgcttcaga
     4681 tatttccgca gcaagcgata accctgcgca ctatgaggat gccattcatt ataatgtttg
     4741 aacaccttcg cgagactact ttactgctgt taacacgtca ggttcgaaca taacactgat
     4801 ttaatcatat ttcatcgttt tgacgaagtt ttctgccatt ccattttgca ccgccgtgtg
     4861 tttgggggca aggccgacca tctgtgtttc attcgccaga tataagtgat gagatggcat
     4921 tctccctcct tcacggcaga agcattttga ccgtttaggg gatgcggacg attttcatgc
     4981 ctgctaagat actttcgtta ctgatgctgt ccgttttcca ccatttcatc atcactgatt
     5041 ggcgttgtgt ttttggtcag cactccagct ttacgtttct cttttaaccg gtaactctct
     5101 cctttgatat tcagcgtgta gcagccgatc taggatcgct gttgccagca cgtgatctcc
     5161 gaacatttct ccccagtctg cgaccccttt gtttgacgtc agtatgatgc tcgctttttc
     5221 atatcgacag ttcatcgata ggaatttaaa tccccaaaag attaaaaaag caccgaagaa
     5281 tgcgcgttta ttcaacacaa cctgatcatc aagtaatcgg tatagacact taatattgct
     5341 tacattgatt gattcaatgt atctctgatg gttttttgct ccgttcccct caacatggac
     5401 gcaacttccc ctctgcgact ctcagccgca ccaccgtatt cgagccagcg gctcatgcat
     5461 cataacctgc tctgccagac ggtaacccct cttcagcccc gtaaaccgca tctgacctcc
     5521 gcgcagctcg cacttcagcg ggtcaacctt cag
//
LOCUS       DAETQL010000176         5548 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.180, whole genome shotgun sequence.
ACCESSION   DAETQL010000176 DAETQL010000000
VERSION     DAETQL010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.180"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            437..2089
                     /locus_tag="K0W65_004030"
     CDS             437..2089
                     /locus_tag="K0W65_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBI3097070.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="K0W65_004031"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="K0W65_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="K0W65_004032"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="K0W65_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3526)
                     /locus_tag="K0W65_004033"
                     /pseudo
     CDS             complement(2596..3526)
                     /locus_tag="K0W65_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(3809..4009)
                     /locus_tag="K0W65_004034"
                     /pseudo
     CDS             complement(3809..4009)
                     /locus_tag="K0W65_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            4387..5136
                     /locus_tag="K0W65_004035"
     CDS             4387..5136
                     /locus_tag="K0W65_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBI3097071.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
     gene            complement(5306..>5548)
                     /locus_tag="K0W65_004036"
     CDS             complement(5306..>5548)
                     /locus_tag="K0W65_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3097072.1"
                     /translation="ELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGR
                     LKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
BASE COUNT         1516 a         1258 c         1236 g         1538 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gaacagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaacgaag cctttaatat cgacttccac cacccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgtaccat ttttcagcgt tccccagaag ctttccatcg
     3841 gagcgttgtc gtaacagtta cctttacgcg acattgatgt tttcagacca aactgctcct
     3901 gtatgacccg gtaatcgtat gcgcagtact gtgaacctcg atcagagtgg tggattagcc
     3961 cggcaggtgg gcgctggctc ctgagcacca taaacagggc tttacccgta aatattggct
     4021 ccttctgatg ccattctatt tcaggaagga gtgtccgtta aactcaggct acctcaaaaa
     4081 gttatcggtg cgtttatcga aaaacatatg ttctactaat tggagtcatt gtctgtaaaa
     4141 gcaactcaaa aatacaagtt gcttattccc cttgttggta agaacttaat tctaactgtt
     4201 tgttctgggt gttttaaacg tcctcctttt tatgcttgtt cataacggat atttaatagg
     4261 taacctttgt tttagcatct tctgatttat ttaaattatg tgatagtaca cacaatttta
     4321 ttatccagta ctttatgctc ttaataaaat aattataaat atgtatcctc tataaggaga
     4381 tgttatatga aaagacagct ttttactctt agtattgtcg gggtattttc tttaaatacc
     4441 tttgcatcat tccctccggg aaatgatgtg acaacaaaac ctgaccttta ctacctgaca
     4501 aacgataatg ctattgacag tctggcatta ttaccgcctc caccacaaat cggaagtatt
     4561 gccttcctga atgaccaggc catgtatgaa aaggggcgtt tattgcgaaa tactgaaaga
     4621 gggaagctgg cagctgagga tgccaatctc agtagtggtg gcgtggcaaa tgtattttct
     4681 gctgcttttg gttctccgat aacggctaaa gattcaccag aattgcataa attactgaca
     4741 aatatgattg aggatgccgg agacctagca acgcgttcag caaaagaata ttatatgcgt
     4801 attcgacctt ttgcttttta tggtgtttct acgtgtaata caaaagaaca ggatacatta
     4861 tccagaaatg gctcttatcc atcaggtcat acatcgattg gttgggcaac agcacttgta
     4921 ttgtccgaaa taaatccggc acgccaggat accattctga aacggggtta tgaactgggg
     4981 gacagcaggg ttatttgcgg ttatcactgg caaagtgatg ttgacgcagc acgtattgtt
     5041 ggctctgcta ttgtagcaac actgcactca aatcctgtgt tccaggcaca attacaaaaa
     5101 gcgaaagatg aattcgctaa caatcagaaa aaataatgct tttagccagt acgccttata
     5161 accccgacct tcagggctaa tgccagtcag ttaagcaact gactggctct ttttcagggc
     5221 tgtggggtat ttccagtgcc tctcctttac cactctcgga aaggcccttt cccttcttgt
     5281 cggtaatttc actaggctgt gtcccttaat tacgtaagcg ttgataaaag agtcggatgc
     5341 agcccagttt cactatcgcc aggtaatttc tcgccgtttt gtcgtaacgc gtggcgatgc
     5401 ggcgatattc tttcagccgc ccaaagcacc gttcaacgac attacgattg cagtacgcat
     5461 cacgatcaag ctgtgcacgt ccatccgatg ccattttctc atttgacttt cgggggatta
     5521 ctgcctttat accgttattt ttcagctc
//
LOCUS       DAETQL010000177         5488 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.181, whole genome shotgun sequence.
ACCESSION   DAETQL010000177 DAETQL010000000
VERSION     DAETQL010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.181"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1631)
                     /locus_tag="K0W65_004037"
     CDS             complement(<1..1631)
                     /locus_tag="K0W65_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HBI3097073.1"
                     /translation="MENSRIPREHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWV"
     gene            complement(1647..2531)
                     /locus_tag="K0W65_004038"
     CDS             complement(1647..2531)
                     /locus_tag="K0W65_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBI3097074.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2542..3147)
                     /locus_tag="K0W65_004039"
     CDS             complement(2542..3147)
                     /locus_tag="K0W65_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBI3097075.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3318..>5488)
                     /locus_tag="K0W65_004040"
     CDS             complement(3318..>5488)
                     /locus_tag="K0W65_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HBI3097076.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1583 a         1363 c         1141 g         1401 t
ORIGIN      
        1 acccacggtt taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg
       61 tgccacggct gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggctagacct
      121 gcgagtgagt tttttacctg ctcattttcg ggaaaaagct catataggcc agagacaatc
      181 gccagcgtag gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt
      241 tcaaaacgca cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca
      301 tttaactcac tgtaatcacg taacaaaata tcgcttacgg caggctcgtt tgccagcgca
      361 tccagtacat agcgtccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg
      421 gcgtggagat cggcaacggc ttgtttaatc aatatttgca aatgggtttt gcgctggcga
      481 ataccgcgcc atccaacact gttcaggtag attttgtcga ttaaacgccc gaatgcgtta
      541 ctgccttgcg gttgattttg atagacataa tccagggaac tgcctgaatc aaagccggta
      601 ctcattccga gatgcaggcc cgcagagtgc gcaccgaaca atttcatcgc ctttcgcata
      661 aagcgatacg ctaaatcaac cggcgataat ggcacgggtc caccagaaag gagccgccat
      721 cgatccgctg atggtcctgt gcggtcttca tgctgataat caaatttttg cgatttgtta
      781 gcatataagc ggcagataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca
      841 cccaatgtgt cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat
      901 ctctgataaa aatcaatctg cggttggcga tgcaccacat agtcatcacc tgatatcaga
      961 agttgcgtgg ggagcgtaat cgccgccgca tcgctaacaa tacgttcaga cgttttatag
     1021 agatcgagca agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc
     1081 gccacccgtt gccgatcgtg ggtcaaatag cgccctttca cataggaatt aataaaaaat
     1141 agaccacgca gacgatgcca taacgccagc gcaggacgtg ccagcggcac atacaattta
     1201 accttaaagg ccggagaagc cagcaccagc ccacgaattg caggtgcata atcatgaacc
     1261 catgtggcaa caagcactgc gccgacgctt tgcgcgatca ctaccacctc ttccagtccg
     1321 acctggctgt cgctggcagc aaaacggaca aattcatcga catcctgcac tgagcgcata
     1381 agagacgggc tataaccacg cggccccgaa ttttttccat gcccccgggc atcccaggca
     1441 taaaaagcag tatcaggcat cgccagttca tcaacgagat gttgtagacg accagaatgt
     1501 tcatgcccgc gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agtgggccag
     1561 tgccgataat acaatgctgt attatcactg gtggtaaaaa aatgttccct agggatgcgt
     1621 gaattttcca ttttctctat tttccttcag taacagcagt agcgaataaa ataaaaaaat
     1681 accggagcgg tgaaaattaa tgagtcaatc ctgtcaagga gtccgccatg tcctagtaat
     1741 agttttccac tatctttaac accaatatct cgtttgatgg ctgacatcac tacatcgccg
     1801 caaaaaccac taataccaat taacaaaccc gctaataatg cctgtaatgt atttagcggt
     1861 gtcagcaacg gtccaataat cagtgacgca atcatggtgg tgattacgcc ccccagcaga
     1921 ccttccagcg tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc
     1981 ccccataaat actgtgcaat atcgtttgac tcggttaacg ccaaaagaaa taacaccagt
     2041 aacgccccgc cctggatatt tatggttggc aacattaata gccaggcggc atgactcaag
     2101 gcaaaaacgg tcattatcca tccccaaaaa atggcgctca cggtatgctg aaaaccggag
     2161 gggtcaccca caaaaactcg ccaggtggct aatatcagaa acccagtcaa aggtataaat
     2221 aacagaaaca gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa
     2281 tataaccaac gcgaaaaatg tacggatata agcgtacaat attctttcaa tgccagaaaa
     2341 cttatcagcg taaaaaacgt caacgtcatc cagcgtgggc ctgaaatcac cagtgaaaaa
     2401 caaatgacga tgccccacca ggtacgaata cgtaatgtaa cttcatcccc ttttctctca
     2461 ggtaaccgcc atagcagaaa acggtttatc agcttgctaa aattaacaac gcgaaaagtg
     2521 ttgccagaga tttttccagc attatctttc agcgctcata agaagcacac tccgacaacg
     2581 attaatcgca gtacagataa gcagaattga agcaatgctc cataaaagat tattccattg
     2641 catccactgt ggataaatgg caacggccag accccacaga ccaaatatta acgcgcgatc
     2701 acttttgcca aatggaccgg cataactgcg aacaccgtta atcgtctgcg cgagtaaacc
     2761 gcaaaactcg gtcaatatgg tgcaaaataa catgagtatc acgagtgatg cgttactttc
     2821 cggtaaaaat aaaaacggta aatagagcgc aatatcggaa ataacatcgc cagtctcatt
     2881 caaaatcgcc cccagacgtg tttgctgatt gcactcacgc gccaacatgc catccagcgc
     2941 attgagcgcc atacggataa aaagcacgat gggcaatagc aaaaagagga tgggttgtgc
     3001 caccaacacc aacagcaatc cggtaaaaag agaaagcgcc agtgccgtaa gagtgatgtg
     3061 attcgctgta acgtggtgct tataaagcca aaacatcgtc ggccttaaca gcgactgaaa
     3121 gagcggtttt atctggtaga gcgtcattat caatccttga taattcggtt tgcgctatgg
     3181 tgcatcaatt cccctgacgg agatatcgca acacgccgct tcattaaaaa ttcagaatat
     3241 atcaaggtat tgggggctca ctgtcaggaa tgattatccc caataaatca agtagttaaa
     3301 aattattacg ctgttattca atacaactca tcaagtttcc tcatcgcttg ttcccgtatt
     3361 gcgcgcgtct taccgttaag cgccagctgg cggactaaat ccagttcagg gttaacaagc
     3421 tctaacgcca gcaaaggatc acgtttcagc ctcgccttca atacatcaac tggaaagcgg
     3481 ggattattca tcgccacaat ccaccactcg ggatcaattt ctgccgccag tgccgcagag
     3541 ggggtgtggc ggttgctaag tgcgccgaac ttgaaaaatg tgagattgct tttcgccagt
     3601 gtactccacc atatacgatc cggttgtttt gccagtacat ccagttgtgc taacgcatcg
     3661 gggtcgagtg cataataaac acgtaatgct tcatcaacct gcgcacggga gctattttcc
     3721 tgcctcagaa aaaacttcgc ccaaatatca ttgcgtggcg tgttttgctg taattccttg
     3781 agaacaacag cgggtaaatt atcccaacta actaatgcac tctctaaaaa gtcccagtct
     3841 tccaaacgta tcagggcggc ggcatttaat agcatctggt catcaacttc gccgtggcta
     3901 atcagctcag acaaaatcat atcagcgctg gtttcatatg catctgaatc tgctttcgga
     3961 ttatttttcc gatgcgataa agcacgttgc caaatttttt gtttcatcga cgtgctcaga
     4021 tactgatttt tcgccagatt actccacccc tcaggatcgt tgaaacgtgt caataaccgt
     4081 tgtaaagcag cgtcagagtg aattacctgc atatcccgac agcaaaagta ggcaggggat
     4141 tttttctcca tccttttaac aaaattaaac tgtaagttat ctggcaatgc taaaaataac
     4201 acacgtatta gatcatcgtc attctcgaat ttttggtata aataggctgc aactttgtca
     4261 ctctcttgtg gcgtcatccg caataattga ccatggtgtt tcatctctgc cagcttgaac
     4321 gctaagcttg tgacaacatt gtttgatgaa tcgttcgcca actgcattgc ctgattgtaa
     4381 gtgagttgtc ctccagcacc gacctgacga atagaggaat catcactgcg gatatagcca
     4441 tcaataatgg attcgggcaa taaccacgca tctttataaa gctcctgaat gatcatatcc
     4501 caatctcttt tcgccagagt catgacgata tccagaggga gattttcacg tagcgcaaga
     4561 ttcaacggac cgacaccatc aggtagacga ctcattcgcg tcattatttc gacagagagc
     4621 ggttcacggt cgagcaaccc cagaacaaca ggctggtaac ccaattgctg atcggtcaac
     4681 tccagaatct cattgtcagg catatatctc gccatacttt caatcgtttg ttcatcattg
     4741 ctattaataa gttgtttgcg taggttagct gaaattcctt taagccttgg gagagttcgc
     4801 attatggcaa gatcaaatgt cgcaatatct cgtccttcaa atgcagcaac gttgccttca
     4861 tccagcattt gcaattcctg cactaactca ggagaaggct tagggcgaaa cagcgcttct
     4921 tttttcatct gattacagac attccctgcc atgcgaatat tgcttcgctt aaaaatttct
     4981 ttcagagcat cttcatcatt tattagaccc caataatatg catcaggatt tattatcatc
     5041 agcgcttttt tgacattttc agtgtcattt ttgtcactcg cacggaccat gaattcacta
     5101 tttgtcgaag acgcaacaac aggtgcaatt atgtcattga taaatacccc acgcgcatta
     5161 atactcccct taatcgtaga cggattatcg taatatgagt cgataaatac cagtccactt
     5221 gcatttatcg aaccggtaat taacagtggt gaatccgtaa ctatattcgt tgccgtcacg
     5281 ttaccgagaa taacccatgg attagaaaga gagaaagtgg aaatattacc tttgactgtc
     5341 aaattgccaa gaacaatcaa tttttgatta ctctctatat taccttctac cactaaatcg
     5401 ccacgatgca ctttccagtc cgatgcagat aaaccgaagg ggacaagatt cacgctactg
     5461 ttgatagctt gttcaacttg ttgtttat
//
LOCUS       DAETQL010000178         5400 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.182, whole genome shotgun sequence.
ACCESSION   DAETQL010000178 DAETQL010000000
VERSION     DAETQL010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.182"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..497
                     /locus_tag="K0W65_004041"
     CDS             <1..497
                     /locus_tag="K0W65_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HBI3097077.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="K0W65_004042"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="K0W65_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="K0W65_004043"
     CDS             2500..3114
                     /locus_tag="K0W65_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBI3097078.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="K0W65_004044"
     CDS             3114..3443
                     /locus_tag="K0W65_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBI3097079.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="K0W65_004045"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="K0W65_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBI3097080.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="K0W65_004046"
     CDS             4529..4840
                     /locus_tag="K0W65_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBI3097081.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4975..5373
                     /locus_tag="K0W65_004047"
     CDS             4975..5373
                     /locus_tag="K0W65_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI3097082.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1178 a         1344 c         1426 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggcaag
//
LOCUS       DAETQL010000179         5350 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.183, whole genome shotgun sequence.
ACCESSION   DAETQL010000179 DAETQL010000000
VERSION     DAETQL010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.183"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(75..557)
                     /locus_tag="K0W65_004048"
     CDS             complement(75..557)
                     /locus_tag="K0W65_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097083.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(625..2574)
                     /locus_tag="K0W65_004049"
     CDS             complement(625..2574)
                     /locus_tag="K0W65_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBI3097084.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2659..2994)
                     /locus_tag="K0W65_004050"
     CDS             complement(2659..2994)
                     /locus_tag="K0W65_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HBI3097085.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2994..3350)
                     /locus_tag="K0W65_004051"
     CDS             complement(2994..3350)
                     /locus_tag="K0W65_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBI3097086.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3350..4846)
                     /locus_tag="K0W65_004052"
     CDS             complement(3350..4846)
                     /locus_tag="K0W65_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBI3097087.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4843..5004)
                     /locus_tag="K0W65_004053"
     CDS             complement(4843..5004)
                     /locus_tag="K0W65_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HBI3097088.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5014..>5350)
                     /locus_tag="K0W65_004054"
     CDS             complement(5014..>5350)
                     /locus_tag="K0W65_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097089.1"
                     /translation="RDEKGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLL
                     DLNRYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEH
                     HLEMRFPQN"
BASE COUNT         1276 a         1561 c         1150 g         1363 t
ORIGIN      
        1 aaagagaagg taaagaaggt aagtccatac tgtctccact ataaaaaaac cgccaatttc
       61 tggcggctta tatattaatc atcattaata tctttcatat tcaatttgca attttcgtag
      121 gtcactcccg actcgccatt aacattaact ccagctctga tattcccgtt ttcttttaca
      181 tctacaaaaa actggcctct tggcatcttg aacgcaaatg taaaaccaac agtatctgaa
      241 tcaaaatcat catctttata tattgggatc aaaaatgtca tcgtcgctgg tgatgcattg
      301 tttgcaaaat caaatacacc tatacttttg ccgaattcat aagaagcttt tctcaatata
      361 atcgcaccgc tggcagaagg ggatgggcac gatatcctca gttgtctgaa tccatctgta
      421 tccaagtcaa cgtcattatc tgcagatgtt ttaacgttac taataaatcg tgaaacaatc
      481 tcctcatttg ttagtatttt cgcctgagca cagcaagagg ttaatattgc aaccgtcatt
      541 aaaaatggct ttatcacaaa acaaactcca ttgttagcat gttacgcgat aattttgcct
      601 catctttcac cacaaaaaaa gaactcacct tttaaatgga gaatacccaa catcatgggt
      661 gattttcata aagggatcgc ctgttttcgg caaatcgata acgcgcattc caggcggcgc
      721 gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa ggcttaacag
      781 ctgattcctt tccagtgata cattcggggt gtagcgttta tcgggagata aatacccatc
      841 accacgtggc atctgccatc cggtcatgtc atagacaaaa tcatggaact gtttgcccca
      901 ctcgtcgaac ttatcattca gaccaaatcc actgttcagt gcatcagcca caaaattttt
      961 aacaagccag ggatgttctt tttcaaactc ctgcgccatc atgcccagtt caagcaatcc
     1021 accaatcaga ctgatctttc cgaggccctt taatttcagc cctgtttttc ctgccgccgt
     1081 tgtccattga ttctgggcga ctgttgccgc acgaatactt gccaccgcct tcacaccaat
     1141 atacagggca gaaaccgttg ctattgtttt tactgcctgt tcccatcccc ccattgctct
     1201 gacaacctga tcaacctcct gccagacttt tttcacaacc ggaccgaccg tttcccagtt
     1261 atcaattatg agatatgcac caccgacgag aagcgcaatt aatccctttg ccggcgtcat
     1321 attcatcacg ctacccatga tttttgttat gcgtgtcaat gtgccaatcg ccacgcccat
     1381 cgtcagtaat gccgcaccag ttttcgcgat ggtttttacg acctcaggat tttctctgac
     1441 aaacgtgcgc acttcttcca gaaaaggctt catctctttt atgccttcat tgagtgaagg
     1501 caggaaggtt tcccccagcg tggaagaaat cgcattgatc tggttctgca gcagcaacaa
     1561 ctgattttct gtcgtcgcgg cacgggcggc atattctttc tgcattgagc cgccatactg
     1621 ctgcgcatcc gccacacgat taaagttggt tcgcaacaaa tccagattcg tgagcagcgg
     1681 cgcaatggcc cccagggatt ccttcccgaa cagcgctttc agcacagcag cctgtttttc
     1741 tttcggcact tttgccagag aatccagtac gtgcagcatg gccccgcggg cgtctttctg
     1801 catatccgcc gccagttttt tcgggctaat gcgcaacgag cggagcactt ttttctgcga
     1861 tttggtggca gaatccctcg ctgtcagcga caacataaaa ttctttatcc ccgtcgcagc
     1921 aatttctgac tccaccccca ttccggcaat ggttgccccc attgcggcaa tctctccgga
     1981 ggccacaccc gcaacactgc ctaaagggcc aatacgggtc acaacatcag aaatcttttt
     2041 cgcacttgca ggaccggtat tgccaagata gttaatctta tccgcaagcc ctgccacctc
     2101 tccctgtgtc agtttaaagg cagtgcgcca ctgcgccatc atctgaccgg attcttctgc
     2161 cgtggtgtca aaggccacgc ccatcttcac ggcatcgtca gtaaactgca tcagctcgtc
     2221 acgggcgata ccagcctgac cacccgccgc cacaatttcg gcgatacctt ccgccgacat
     2281 gggcagttct gttgacaggt cgcgcacctg ctccgtcatt gccttaaacg cttccggcgt
     2341 atccagacca tccaccactt tccggacatc cgccattttt gattcaaggg caatggcaga
     2401 tttgaccggg agcgccagcg cccccagtac tgcggttccg gcacccgctg cgcccagtga
     2461 caggctggca aactcctttt taaaaccttt cagctggcgc tgcattccct tcagcggtgc
     2521 cgatacctta tccacggcag tgatgatcgc cttcagctga aaactgtcag ccatgcttca
     2581 tctcctcgtt aatgcggacg gcctcggctt ccagttctgt aaactgcgaa acagccaccc
     2641 gtcgcagctc cagtggattc agtttccaga accaggcaac attgtagagt cgtttccgga
     2701 ggtgcttccc gtctccgact gggtaaaaaa acgcaggatc tgcatgctgg tcttaaaaat
     2761 atccagtttt gccatctgcg ccgccgatga acacgggatc cccgccagca acgggatata
     2821 tttcagtgcc acctggctgt ccagtttaat actgccttca ccggaaataa tgaaaggaaa
     2881 acccagcgcc tcgatttcgt catacgtggg ctcgcgcaac tccagcacat gcagcatttc
     2941 gttatgcgct gtcaccggtt tttttaaaac tatttctgta acgttcattg ccattactga
     3001 taccctccct cttcgccgtg gaattcaaga tctgccgtgc cttcttcggc attatgatta
     3061 gcctctccgt gcagccatgc cgccgaaagc acatacacca tgccgtttgc cagctcagcg
     3121 gtaatcgtca tctggtctga ggtggtaatt ttgtcgaccg gaaagttttt gggcacttta
     3181 aacgtgccct taacgtaagg tgaccgccag gtctccttgt aatccacatc ccctgccatg
     3241 ccgacgatgt catcccggac attggtgttc atcggcactt caatgccacc tgtcagcgat
     3301 aactgctgac catccacttt aaaaaaacag gttccggcga tctttgccat tatgctgact
     3361 cctctgaata ctgaagacgg aactggttaa ccaccgcaaa gacacgtaac tgattaacat
     3421 aatccggcgg gaacagcgta ttcagtcggt tcgggttatc tgcatcacgc tcaactatca
     3481 gatactgttt aaacagatcg taattttcca caataccggc acgctccatc tgacgatatg
     3541 tcgccagaag ttcccctttg ataacggcag gagtgacaat cgcctgcccc ggaccaaaac
     3601 gggtaccatc atttgccagc ttgtgacgtc cgtacttgct tgtgatgacc gatttcagtt
     3661 tgcgaagaac atatgcactg gtatgcagag tttcactgtc cagatagctg ttgtctgcca
     3721 cgccatacgc attctttttg taggtggtta cggaacgctg gatctgcaac gtgccgcctt
     3781 ccacataagc cgtcgccacg ccgtgagata aaagggtctg ctgctctgtc atgatgaatc
     3841 gcttaccttt cggtgccgga agcatcccca ccagctcccc cgtctgtgtc ggacgggcag
     3901 gatcattccg gataaatacc gcttcacggg caaggcgact ggcaaccagt tcatcgacag
     3961 gcgactgggt ttctttttcg taaccggcaa gcgtgatatg ttgctgatta tgcatatctc
     4021 cggcaccaac cagctctgac agcgttccca gttttgcggt atagacatgc ccgtataact
     4081 ggcgcgcata actccagcga ccgctgctgt cattcatttc ggtcatcatc atattgatgg
     4141 aggcggcatc gttgaacggc agaccgataa agtcgaatgc ctcatcgccc atagcagcaa
     4201 cagcggcggt aagatcaggc gctccgctac cggcagcacc gacttccgtc accacccgaa
     4261 gccctgctgg caaaatctca ccaccaccag aaccataata attcagacag accggtaact
     4321 cattaccata cagcccctta tgacgggcag tcagcgtcac cacgcctgca tcagatgacg
     4381 ccgtaaacgg cagggtgata accccgttta ccgcttcctt aatcgcggtg gcaaccgcag
     4441 tggcatcatc gccattcacc acaggcacct gtacacggga gcgcccgaca tacagactca
     4501 gggtgccgct ttcctctgct tctccggcaa ccgtcaccct gaccgtcgcc gccgcccctc
     4561 tggcttccgg tactgcaata acatacagtt caccgaaagg atctgtctga cggtagactt
     4621 ccaccatacg cgccagctgg ctccccgccc cacaaatctg gcgggcataa tctgccgatg
     4681 gcatcagcac caggctgtta acctcaatgg ctgcatcgtt gctggcatgc ccgatcagta
     4741 atgcaggcgc gctggtcacc gctgtatttg ccgcagaatt gttcatctcg gcgtagaaca
     4801 acggcaccag cgtattcgac ggaacagtat taaaacttat cgtcatgatt ttttagcctt
     4861 atcctgttta acacgtacca catcacccgc cgctatacgg cggaaccagt agctgctttc
     4921 ttccacattt cgcccttcgg aaggcaaaag gtctccacgg gcagggtcag gaaccgaccg
     4981 ccctttcagg ggtttcacaa acatgaggga ctcttaattc tgagggaaac gcatttccag
     5041 atggtgctca atgtcaccat ccgggccagt accaggatcg atgtaatcca catcaatact
     5101 cagcagggat aaatccggca gggcattcac gtcctctgcc tgtcgcgtgt cttcttccgt
     5161 gatttcatac ttcgccgtaa aatcaaactg gtaatacagt tcgtaacggt tcagatccag
     5221 cagggtgcca ccgacataaa caatctcgcc cccttgcggg tccggcatcc atccaagcag
     5281 ggctttccag agttctctcc ggacatcatg aacggcgtca taggctgccc actgcccttt
     5341 ttcatcacgc
//
LOCUS       DAETQL010000180         5340 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.184, whole genome shotgun sequence.
ACCESSION   DAETQL010000180 DAETQL010000000
VERSION     DAETQL010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.184"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="K0W65_004055"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="K0W65_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBI3097090.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKGRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="K0W65_004056"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="K0W65_004056"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBI3097091.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="K0W65_004057"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="K0W65_004057"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBI3097092.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="K0W65_004058"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="K0W65_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBI3097093.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1291 c         1458 g         1281 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagcggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 tgcggttaac gacggcaacc catgcgccac gttctgtata gtactcggcc acggagtatt
      241 catatttcgc cagacgacct ttcaggaata ccagacgttt ggtggcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgcg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 acgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggc ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gcacccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tccctctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tagtggtaca tcctggcgcg ggtaacccgg atggcacggt actgaatgcg
     1261 ttgcttcatt actatccgcc cattgccgat gtaccacgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaacggcg tgaaattact cgtgagtatg aagcggtggc gattggtcat
     1441 atgaccgctg gcggtacagt agatgaaccc atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atccgatggg caaaccggcg gtgactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaactggac gtacgcacca gatccgcgtg
     1621 catatggccc atatcactca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcatttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ccatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cacaagatat ggtggagctg attgaggtga tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tccactcgta tcggcggcgt gagcttgccc ccgtatgact
     1981 cactcaacct cggtgcccat tgtggcgata acccggatca cgttgaggag aatcgcaagc
     2041 gactttttgc tgcgggcaat ttgccttcta aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagcttact ggcgaacctt atgcctcaaa acgagcagat gcctcttata
     2161 gcaatacgcc tggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgatccggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgtggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcaac aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaga
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gatgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg aatttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaatat tctgttgcag gtactggatg atgggcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatctgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtta
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg
//
LOCUS       DAETQL010000181         5239 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.185, whole genome shotgun sequence.
ACCESSION   DAETQL010000181 DAETQL010000000
VERSION     DAETQL010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.185"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..141
                     /locus_tag="K0W65_004059"
     CDS             <1..141
                     /locus_tag="K0W65_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3097094.1"
                     /translation="RNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRL
                     RN"
     gene            400..584
                     /locus_tag="K0W65_004060"
                     /pseudo
     CDS             400..584
                     /locus_tag="K0W65_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            727..948
                     /locus_tag="K0W65_004061"
                     /pseudo
     CDS             727..948
                     /locus_tag="K0W65_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(969..1031)
                     /locus_tag="K0W65_004062"
                     /pseudo
     CDS             complement(969..1031)
                     /locus_tag="K0W65_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1481..2893
                     /gene="ospC1"
                     /locus_tag="K0W65_004063"
     CDS             1481..2893
                     /gene="ospC1"
                     /locus_tag="K0W65_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HBI3097095.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2997..3146)
                     /locus_tag="K0W65_004064"
                     /pseudo
     CDS             complement(2997..3146)
                     /locus_tag="K0W65_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            3222..4919
                     /gene="ospD3"
                     /locus_tag="K0W65_004065"
     CDS             3222..4919
                     /gene="ospD3"
                     /locus_tag="K0W65_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HBI3097096.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            5036..5155
                     /locus_tag="K0W65_004066"
                     /pseudo
     CDS             5036..5155
                     /locus_tag="K0W65_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
BASE COUNT         1767 a          981 c         1012 g         1479 t
ORIGIN      
        1 cgtaatgtcg ttgaacggtg ctttgggcgg ctgaaagaat atcgccgcat cgccacgcgt
       61 tacgacaaaa cggcgagaaa ttacctggcg atggtgaaac tgggctgcat ccgactcttt
      121 tatcaacgct tacgtaatta agggacacag cctagtctcc aggattcccg gggcggttca
      181 atcaaagcgc agaacttctg cgtcttcttc gtcaaaaaaa tcatcttcgt gaagcttcac
      241 gacatagcgg ggaagtttgc ttctttgaga ggcgggttta cgtttacggg atttagctga
      301 acgggccata taaccacctg aaagacaatg acattgcctg tttttataac ggtaattgca
      361 gaccatgaca aaccgcagcc gtcaggctgc ctactcgagt cacaaggctc tttgtgtccg
      421 atatgccggc agcgcattac ccatgaaact ggctggaacg tacaccataa ggtgaagaaa
      481 gttatggggg cggtgaagaa ttatctaacc ttgtcttgtt acatccaaat tgccatcgac
      541 agttacacag ttgtgaagcc ggctctctca aaaagggctt ataaaggctt gagcggtgta
      601 ccgggaaacc ggtatgcacc gttcttaggg gagggcagtc cagcaatgaa ctgtccttac
      661 ccgacaaaca tccaaaacga acggaacgtg ctggaatccg catataaccc gttgtaaagt
      721 gaggaaatgg acacctcact tgctcatgag aacgcccgcc tgcgggcact gttgcagacg
      781 caacaggaca ctatccacca gatggctgaa tacaaccgcc tgctctcaca gcggatggcg
      841 gcttatgctt ccgaaatcaa ccgactgaag gcgctggttg cgaaactgca gcgtatgcag
      901 ttcggtaaaa gctcagaaaa acttcgcgca aaaaccgaac ggcagattcc attttcacgg
      961 gcgatctacg ccacacaggc tctgggttgc agtgactgct ccattaaggc gattttgaac
     1021 tcataaggga agtaacaaaa aaactgcaaa agtacaataa ttatatatgg ccaaactaac
     1081 ctctattaaa aaacatcagt ttttcacact tagttgagct tcatgaaaaa atcatcgagg
     1141 atttcattgc agaatacaca ttccactaac tggaagcact ccataagagg aatcagagaa
     1201 agtatcgttt ttttatagta aaattcattg ctgttcaata aaattgatat agttattaaa
     1261 ttatgaaaat aatttcacaa aaaagattaa cttgagttct taagcatcgg attttagata
     1321 ttttgaaata tctggcatca cataacatca aaagtatata atactcttat ctaattacat
     1381 gcagtatatt gtatatatac tcaaaaattg taattcatac cctgctatat cttgtaggga
     1441 aagatattaa ttaaaactgt tttcatataa ggttcatttt atgaatatat cagaaacact
     1501 gaactcagca aatacccaat gcaatataga ttctatggat aacagattac atacattgtt
     1561 tccaaaagtg acatcagtgc gaaacgctgc acaacaaact atgccagatg aaaaaaattt
     1621 aaaagatagt gcaaatatta ttaaaagttt ctttaggaaa actatagcag cacagagtta
     1681 tagtagaatg ttctctcaag gctctaactt taaatcttta aatatagcaa ttgatgcacc
     1741 atcagacgct aaagcctcat ttaaggctat tgagcacctt gacagattat cgaagcatta
     1801 tatatctgaa ataagggaaa aacttcatcc tctttctgca gaggaactca atttgctttc
     1861 gctaattatt aattctgatt taatcttcag acatcaaagt aattctgatt tgtctgataa
     1921 aattttaaac attaagtcat tcaataaaat tcagtctgaa ggaatatgca caaaacgaaa
     1981 cacatacgct gatgatataa aaaaaatagc taatcatgac tttgtgtttt ttggcgttga
     2041 aatctctaac catcagaaaa aacaccccct gaatacaaaa catcacactg ttgattttgg
     2101 tgcaaatgcg tatatcattg atcatgactc tccatatgga tatatgacat taaccgatca
     2161 ctttgataat gctattccac ctgtttttta ccatgagcac caatcatttt tagataaatt
     2221 ttcagaggtt aataaagaag ttagtcgata cgtacatgga agtaaaggaa ttatagatgt
     2281 accaatattc aatactaaag atatgaagtt agggctcgga ttatacctga ttgactttat
     2341 tagaaaaagt gaagaccaaa gcttcaagga gttttgctat ggaaaaaatc ttgcccctgt
     2401 ggatctggat agaatcataa actttgtttt tcagccagag taccatatac ctaggatggt
     2461 aagtacagaa aacttcaaaa aagttaagat tagagaaata tccttagagg aggctgttac
     2521 agcatctaat tacgaagaaa ttaacaagca ggtcactaac aaaaaaattg ctctccaggc
     2581 tctttttctt tcgattacta atcaaaaaga ggatgtcgcc ttatatatat tatctaattt
     2641 tgagataact agacaagatg ttatttccat aaagcatgag ttgtatgata ttgagtatct
     2701 acttagcgct cataattcaa gctgtaaagt acttgagtat tttatcaata agggattggt
     2761 tgatgtaaac acaaagttca aaaaaactaa tagtggggat tgtatgttgg ataacgcaat
     2821 aaaatatgag aatgcagaaa tgataaaact attattgaaa tatggtgcaa catctgacaa
     2881 taaatatatt taatcaaaat tgaatatcgt ttagcaaaag gcagtatttg cacaaaatat
     2941 cgatatattg tttattgtca gtatggctca atgtgataat agttggaaag tttgatgggt
     3001 ttcgccccgt tgtagcggta gtcgaactgg aaggtcttca ggcactgctt acagcgatag
     3061 agcagccccc ctgaactgga atggccgttc cggatacccc cctgagtttc agagtaacgg
     3121 tgacaaacca catcaatctt tgccatcaat catccaaagg gcaaagagta caacaacact
     3181 aagtctgcgt cacaacccat caatgaaagg aatatataca tatgccatca gtaaatttaa
     3241 tcccatcaag gaaaatatgt ttgcaaaata tgataaataa agacaacgtc tctgttgaga
     3301 caatccagtc tctattgcac tcaaaacaat tgccatattt ttctgacaag aggagttttt
     3361 tattaaatct aaattgccaa gttaccgatc actctggaag acttattgtc tgtcgacatt
     3421 tagcttccta ctggatagca cagtttaaca aaagtagtgg tcacgtggat tatcatcact
     3481 ttgcttttcc ggatgaaatt aaaaattatg tttcagtgag tgaagaagaa aaggctatta
     3541 atgtgcctgc tattatttat tttgttgaaa acggttcatg gggagatatt attttttata
     3601 ttttcaatga aatgattttt cattccgaaa aaagcagagc actagaaata agtacatcaa
     3661 atcacaatat ggcattaggc ttgaagatta aagaaactaa aaatgggggg gattttgtca
     3721 ttcagcttta tgatcccaac catacagcaa ctcatttacg agcagagttt aacaaattta
     3781 acttagctaa aataaaaaaa ctgactgtag ataattttct tgatgaaaaa catcagaaat
     3841 gttatggtct tatatccgac ggtatgtcta tatttgtgga cagacatact ccaacaagca
     3901 tgtcctccat aatcagatgg cctgataatt tacttcaccc caaagttatt tatcacgcga
     3961 tgcgtatggg attgactgag ctaatccaaa aagtaacaag agtcgtacaa ctatctgacc
     4021 tttcagacaa tacgttagaa ttacttttgg cagccaaaaa tgacgatggt ttgtcaggat
     4081 tgcttttagc tttacaaaat gggcattcag atacaatctt agcatacgga gaactcttgg
     4141 aaacttctgg acttaacctt gataaaacgg tagaactact aactgcggaa ggaatgggag
     4201 gacgaatatc gggtttatcc caagcacttc aaaatgggca tgcagaaact atcaaaacat
     4261 acggaaggct tctcaagaag agagcaataa atatcgaata caataagctg aaaaatttgc
     4321 tgaccgctta ttattatgat gaagtacaca gacagatacc cggactaatg tttgctcttc
     4381 aaaatggaca tgcagatgct atacgcgcat acggtgagct cattcttagc ccccctctcc
     4441 tcaactcaga ggatattgta aatttgctgg cctcaaggag atatgacaat gttcccggac
     4501 ttctgttagc attgaataat ggacaggctg atgcaatctt agcttatggt gatatcttga
     4561 atgaggcaaa acttaacttg gataaaaaag cagagctgtt agaagcgaaa gattctaatg
     4621 gtttatctgg attgtttgta gccttgcata atggatgtgt agaaacaatt attgcttatg
     4681 ggaaaatact tcacactgca gaccttactc cacatcaggc atcaaaatta ctggcagcag
     4741 aaggcccaaa tggggtatct ggattaatta tagcttttca aaataggaat tttgaggcaa
     4801 taaaaactta tatggaaata ataaaaaatg aaaatattac acctgaagaa atagcagaac
     4861 acttggacaa aaaaaatgga agtgattttc tagaaattat gaagaatata aaaagctgaa
     4921 tattatctcg tggtgataca ctgaatttga ggtaaccggc tcatttaaac cgtctggtct
     4981 gttacctccg gttccacaaa aataatgtcc atcattttga agtggacact atcgtatgga
     5041 ataccggacc tggatcactg aagctttacg ccttcatttc gaagaacatt taccacgggt
     5101 tgtggccgga cgtcgcctgg gcgtaccaaa atcaactgtc tgtggtatgt tcgtgtgaac
     5161 cgcccccggt atcccgctca tgctggcgaa cccagacacg cagtgaaccg ccccgggttt
     5221 cctggagagt gttttatct
//
LOCUS       DAETQL010000182         5200 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.186, whole genome shotgun sequence.
ACCESSION   DAETQL010000182 DAETQL010000000
VERSION     DAETQL010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.186"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..117)
                     /locus_tag="K0W65_004067"
     CDS             complement(<1..117)
                     /locus_tag="K0W65_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3097097.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTW"
     gene            224..298
                     /gene="tnaC"
                     /locus_tag="K0W65_004068"
     CDS             224..298
                     /gene="tnaC"
                     /locus_tag="K0W65_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HBI3097098.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            519..1934
                     /gene="tnaA"
                     /locus_tag="K0W65_004069"
     CDS             519..1934
                     /gene="tnaA"
                     /locus_tag="K0W65_004069"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HBI3097099.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            2025..3272
                     /gene="tnaB"
                     /locus_tag="K0W65_004070"
     CDS             2025..3272
                     /gene="tnaB"
                     /locus_tag="K0W65_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HBI3097100.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3404..4579
                     /gene="mdtL"
                     /locus_tag="K0W65_004071"
     CDS             3404..4579
                     /gene="mdtL"
                     /locus_tag="K0W65_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBI3097101.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4554..>5200
                     /gene="yidZ"
                     /locus_tag="K0W65_004072"
     CDS             4554..>5200
                     /gene="yidZ"
                     /locus_tag="K0W65_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBI3097102.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1198 a         1251 c         1298 g         1453 t
ORIGIN      
        1 ccatgtttta cggcaatgag agcagagata gcgctgatgt ccggcagtgc ttttgccgtt
       61 acgcaccacg ccttcagtag cggagcagga aggacatctg atggaaatgg aagccacgca
      121 agcaccttaa aatcaccatc atacactaaa tcagtaagtt ggcagcatta ccaaattctg
      181 agtgtaataa tgtagcggcg tgtctttcga ggataagtgc attatgaata tcttacatgt
      241 atgtgtgacc tcaaaatggt tcaatatcga caataagatt gtcgatcacc gcccttaatt
      301 tacccttctg tagccatcac cagagccaaa ccgattagat tcaatgtgat cgattagttt
      361 gctatatctt aattttgcct tttgcaaagg ccatctctcg tttatttact tattttagta
      421 aatgatggtg cttgtatata tacatggtga attaatggat attgcagatg taatattcac
      481 agggatcact gtaattaaaa taaatgaagg attatgtaat ggaaaacttt aaacatctcc
      541 ctgaaccgtt ccgcattcgt gttattgagc cagtaaaacg tactactcgc gcttatcgtg
      601 aagaagcaat tattaaatcc ggtatgaacc cgttcctgct ggatagcgaa gatgtgttta
      661 tcgatttact gaccgacagc ggcaccgggg cggtaaccca aagtatgcag gcagcgatga
      721 tgcgcggcga cgaagcctac agcggcagcc gcagctacta tgcgttagcc gagtcagtga
      781 aaaatatctt tggttatcaa tatactattc cgactcacca gggccgtggc gcagagcaaa
      841 tctatattcc ggtactgatt aaaaaacgcg agcaggaaaa aggcctggat cgcagcaaaa
      901 tggtggcatt ctctaactat ttctttgata ccacgcaggg ccatagccag attaacggct
      961 gtaccgtgcg taacgtctat atcaaagaag ccttcgatac gggcgtgcgt tacgacttta
     1021 aaggcaactt tgacctcgaa ggattagaac gcggtattga agaagttggc ccgaataacg
     1081 tgccgtatat cgttgcaacc atcaccagta actctgcagg tggtcagccg gtttcactgg
     1141 caaacttaaa agcgatgtac agcatcgcga agaaatacga tattccggtg gtaatggact
     1201 ccgcacgctt tgctgaaaac gcctatttca tcaagcagcg tgaagcagaa tacaaagact
     1261 ggaccatcga gcagatcacc cgcgaaacct acaaatatgc cgatatgctg gcgatgtccg
     1321 ccaagaaaga tgcgatggtg ccgatgggcg gcttgctgtg catgaaagac gacagcttct
     1381 ttgatgtgta caccgagtgc agaacccttt gcgtggtgca ggaaggcttc ccgacatatg
     1441 gcggcttgga aggcggcgcg atggagcgtc tggcggtagg tctgtatgac ggcatgaatc
     1501 tcgactggct ggcttatcgt atcgcgcagg tacagtatct ggtcgatggt ctggaagaga
     1561 ttggcgttgt ctgccagcag gcgggcggtc acgcggcatt cgttgatgcc ggtaaactgc
     1621 tgccgcatat cccggcagac cagttcccgg cacaggcgct ggcgtgcgag ctgtataaag
     1681 tcgccggtat ccgtgcggta gaaattggct ctttcctgtt aggccgcgat ccgaaaaccg
     1741 gtaaacaact gccatgcccg gctgaactgc tgcgtttaac cattccgcgc gcaacatata
     1801 ctcaaacaca tatggacttc attattgaag cctttaaaca tgtaaaagag aacgcggcga
     1861 atattaaagg attaaccttt acctacgaac caaaagtatt gcgtcacttc accgcaaaac
     1921 tgaaagaagt ttaattaata ctacagagtg gctataagga tgttagccac tctcttaccc
     1981 tacatcctca ataacaaaaa tagccttcct ctaaaggtgg catcatgact gatcaagctg
     2041 aaaaaaagca ctctgcattt tggggtgtta tggttatagc aggtacagta attggtggag
     2101 gtatgtttgc tttacctgtt gatcttgccg gtgcctggtt tttctggggt gcctttatcc
     2161 ttatcattgc ctggttttca atgcttcatt ccgggttatt gttattagaa gcaaatttaa
     2221 attatcctgt cggctccagt tttaacacca tcaccaaaga tttaataggt aacacctgga
     2281 acattatcag cggtattacc gttgccttcg ttctctatat cctcacttat gcctatatct
     2341 ctgctaatgg tgcgatcatt agtgaaacga tatcaatgaa tttgggctat cacgctaatc
     2401 cacgtattgt cgggatctgc acagccattt tcgttgccag cgtattgtgg ataagttcgt
     2461 tagccgccag tcgcattacc tcattgttcc tcgggctgaa gattatctcc tttgtgatcg
     2521 tgtttggttc tttcttcttc caggtcgatt actccatcct gcgcgatgcc accagcacca
     2581 ctgcgggaac gtcttacttc ccgtatatct ttatggcttt gccggtgtgt ctggcgtcat
     2641 ttggtttcca cggcaatatt cccagcctga ttatttgcta tggcaaacgc aaagataagt
     2701 taatcaaaag cgtggtattt ggttcgctgc tggcgctggt gatttatctc ttctggctct
     2761 attgcacgat ggggaatatt ccgcgcgaaa gctttaaggc gataatctcc tcaggcggca
     2821 acattgattc gctggtgaaa tcgttcctcg gcaccaaaca gcacggcatt atcgagtttt
     2881 gcctgctggt gttctctaac ttagctgttg ccagttcgtt ctttggtgtc acgctggggt
     2941 tgttcgatta tctggcggac ctgtttaaga ttgataactc ccacggcggg cgtttcaaaa
     3001 ccgtgctgtt aaccttcctg ccacctgcgt tgttgtatct gatcttcccg aacggcttta
     3061 tttacgggat cggcggtgcc gggctgtgcg ccaccatctg ggcggtcatt attcccgcag
     3121 tgcttgcaat caaagctcgc aagaagtttc ccaatcagat gttcacggtc tggggcggca
     3181 atcttattcc ggcgattgtc attctctttg gtataaccgt gattttgtgc tggttcggca
     3241 acgtctttaa cgtgttacct aaatttggct aaatccttca agaagccagc cattcgctgg
     3301 cttcttgcct ctcaggaaat cacttatgtc caaatggcaa ctcgccttat cctccttcac
     3361 cacgtatgct ttgcgtcacc ttactatcag gacgctttag cccatgtccc gctttttgat
     3421 ttgtagtttt gccctggttt tactttatcc cgccgggatt gatatgtacc tcgttggttt
     3481 accgcgcatc gccgccgatc tcaatgccag cgaagcgcag ttgcatattg cgttctccgt
     3541 atatctggcg gggatggcag ctgcgatgct atttgccggt aaagtggccg atcgttcagg
     3601 gagaaagccg gtcgccatac ccggcgcggc gctatttatt attgcctcgg tgttctgttc
     3661 actggctgaa accagcacgt tatttcttgc aggccgattt ctacaggggt tgggcgcagg
     3721 ctgttgttac gtggtggcgt tcgccatttt gcgcgacacg ctggatgatc gacgtcgggc
     3781 taaagtgctg tcattactca acggtattac ctgcatcatt ccggtgttag cgccagtgct
     3841 cggacatctg attatgctta aattcccgtg gcagagtctg ttctgggcga tggcaatgat
     3901 gggcatcgcg gtactgatgt tgtctttgtt tattttaaaa gaaacgcgcc cagcgtcccc
     3961 cgctgcttcg gacaaaccac gagaaaatag cgagtcgctg cttaatcgtt ttttcctcag
     4021 ccgtgttgtt atcaccaccc tcagcgtttc ggtgatcctc actttcgtca atacatcgcc
     4081 ggtattgctg atggaaatca tggggtttga gcgcggagaa tacgccacca ttatggcgct
     4141 gaccgctggc gtcagcatga ccttttcatt ctcgacgcca tttgcgctgg gaatttttaa
     4201 gccacgtacg ttgatgatca cctcgcaggt gttattcctt gcggcgggga tcacccttgc
     4261 cgtttcacct tcccatgcgg tttctctgtt tggtatcacg ctgatttgcg ccggtttctc
     4321 ggtaggtttt ggcgtagcga tgagtcaggc gttaggacca ttttcattac gcgcgggcgt
     4381 agccagctcg accttaggta ttgcgcaggt ttgcggttcg tcactgtgga tttggctggc
     4441 cgcggtggtt ggtatcggcg catggaatat gctgatcggg attctgattg cctgtagcat
     4501 agtgagcctg ttgctgatta tgttcgtcgc gcctggacgc cccgttgccg ctcatgaaga
     4561 aatccatcac cacgcttgat ctcaatctgc tgctctgtct gcaactgctg atgcaggagc
     4621 gcagcgtgac caaagcggcg aagcggatga acgtgacccc ttcagcggtg agtaagtcgc
     4681 tggcaaagtt aagagcgtgg tttgacgacc cgctctttgt gaactcaccg ctggggctgt
     4741 cgcccacaca gctaatggtc agcatggagc aaaatctggc ggaatggatg caaatgagca
     4801 atctgctgct ggataaaccg caccaccaga caccgcgcgg cctgaagttt gagttggcgg
     4861 cggaatcgcc gctgatgatg atcatgctta atgcgctgtc gaaacggatc taccaacgtt
     4921 acccgcaggc gaccatcaaa ctacgtaact gggattacga ttccttagat gccattactc
     4981 gtggtgaagt ggatattggt ttttccggtc gcgaaagcca tcctcgctcg cgggagctgt
     5041 taagctcgct accgttagcc attgattatg aagtgctgtt tagtgatgta ccctgcgtct
     5101 ggttacgcaa agatcatccg gcactgcatg aaacgtggaa tctggacacc tttttacgtt
     5161 atccgcatat cagcatttgc tgggaacaga gcgacacctg
//
LOCUS       DAETQL010000183         5182 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.187, whole genome shotgun sequence.
ACCESSION   DAETQL010000183 DAETQL010000000
VERSION     DAETQL010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.187"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..746
                     /locus_tag="K0W65_004073"
     CDS             <1..746
                     /locus_tag="K0W65_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3097103.1"
                     /translation="VDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWN
                     NVTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIAT
                     LGSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKL
                     SFYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPL
                     GLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1080..3359
                     /gene="ydeP"
                     /locus_tag="K0W65_004074"
     CDS             1080..3359
                     /gene="ydeP"
                     /locus_tag="K0W65_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBI3097104.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3607..3810
                     /gene="safA"
                     /locus_tag="K0W65_004075"
     CDS             3607..3810
                     /gene="safA"
                     /locus_tag="K0W65_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HBI3097105.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3878..4639
                     /gene="ydeO"
                     /locus_tag="K0W65_004076"
     CDS             3878..4639
                     /gene="ydeO"
                     /locus_tag="K0W65_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HBI3097106.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5041..>5182
                     /locus_tag="K0W65_004077"
     CDS             5041..>5182
                     /locus_tag="K0W65_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBI3097107.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDF"
BASE COUNT         1481 a         1102 c         1156 g         1443 t
ORIGIN      
        1 ttgtagactt gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg
       61 atcatataaa cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat
      121 cgctgtactg gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg
      181 atattggtga taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg
      241 cggccggtgg agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa
      301 ttgcaacatt aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca
      361 gtgttgttat gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc
      421 cggactttcc tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc
      481 tctcatttta tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag
      541 cacctgatgc aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac
      601 tggctacggg agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt
      661 taagcgcaac ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca
      721 ttggtgtcac ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc
      781 acgaaaattg cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc
      841 cgcgcacaat tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac
      901 tatgagagat gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa
      961 aatagaaaga gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat
     1021 gtactgtgca tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga
     1081 tgaagaaaaa aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg
     1141 tagcgaatgc agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca
     1201 tgaataagcc agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg
     1261 cgtcattcga catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc
     1321 aggtaaatgc ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc
     1381 acgagcttga ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact
     1441 gttacaagcc attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct
     1501 atagtgatcc caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct
     1561 ttctttatca gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca
     1621 tgtgccatga accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg
     1681 tgttgctgga agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta
     1741 caaaccaccc tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga
     1801 tcgccatcaa tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt
     1861 ttgaaatgct gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg
     1921 gtggcgatat ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg
     1981 caagtgccgc aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg
     2041 gctttgacga gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt
     2101 ctggactaag tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca
     2161 ccattatctg ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc
     2221 aactggtcaa tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc
     2281 cactacgtgg tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt
     2341 ctgcagagtt tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg
     2401 gacatgctgc aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct
     2461 gcatgggggg caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa
     2521 cgcaattaga tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg
     2581 cacggcatag ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg
     2641 gtgcgcaggc ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt
     2701 taaaacccgc cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg
     2761 cagcactacc ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc
     2821 gcaatgacat tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc
     2881 ccggtggttt tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta
     2941 aggctaattt cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta
     3001 agctggtcat ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg
     3061 atgatcgcta tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag
     3121 ctaaaatttg ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg
     3181 gtaagcgcag ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc
     3241 gctcactggt gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc
     3301 cattaagtgg tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat
     3361 gcctcttctc atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta
     3421 ttgcgcaatt tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc
     3481 ctacagctgt aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc
     3541 agcctgttat attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata
     3601 tttgaaatgc atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa
     3661 atcatgtctg taattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg
     3721 gcaattgatc agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc
     3781 attatcaata gtttgcaaga ctggaaataa acagcaaatt ataaatgtga aatgtttaaa
     3841 aggtatcgat aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat
     3901 atttattcat catgctttca acgctaacat tttagataaa gattacgcct tctccgacgg
     3961 cgagatcctg atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt
     4021 taaagaaatc ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca
     4081 gacagtgacg gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt
     4141 gcttaatgaa atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga
     4201 acttttgctt ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt
     4261 aactaacggt gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc
     4321 gcacccatgg aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa
     4381 gaaaaaacta aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca
     4441 gcacgcaaaa aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg
     4501 ttatgccagt acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa
     4561 gagagtttct aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat
     4621 gagtgcttta gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga
     4681 attatatggg gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat
     4741 aaataagtga catccatcac atatttatgc acttgcataa cctgttgcat gattatttat
     4801 gatctcaatt cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag
     4861 ttgtttatcg gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact
     4921 gttatttccc tttttttagc taaatctgct atctctttat gtgactaact tcacttacat
     4981 ccacttattt ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt
     5041 atgaagtctg cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct
     5101 ggtatggctg catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca
     5161 aacgttgctt tctcagactt ta
//
LOCUS       DAETQL010000184         5164 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.188, whole genome shotgun sequence.
ACCESSION   DAETQL010000184 DAETQL010000000
VERSION     DAETQL010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.188"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..220
                     /locus_tag="K0W65_004078"
     CDS             <1..220
                     /locus_tag="K0W65_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3097108.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(392..721)
                     /locus_tag="K0W65_004079"
     CDS             complement(392..721)
                     /locus_tag="K0W65_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBI3097109.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(726..911)
                     /locus_tag="K0W65_004080"
     CDS             complement(726..911)
                     /locus_tag="K0W65_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBI3097110.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(908..3547)
                     /locus_tag="K0W65_004081"
     CDS             complement(908..3547)
                     /locus_tag="K0W65_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBI3097111.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWLYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3755..4744
                     /gene="ldhA"
                     /locus_tag="K0W65_004082"
     CDS             3755..4744
                     /gene="ldhA"
                     /locus_tag="K0W65_004082"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBI3097112.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4855..>5164
                     /gene="hslJ"
                     /locus_tag="K0W65_004083"
     CDS             4855..>5164
                     /gene="hslJ"
                     /locus_tag="K0W65_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HBI3097113.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1309 a         1322 c         1203 g         1330 t
ORIGIN      
        1 tttgtaccga atgtttgccg ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg
       61 tctcgatttt tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat
      121 cggctttcat tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt
      181 tgggatgacg ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgcatgtac
      241 tgcaacgcca attagttaaa acagatattt gactatgcat ttttcagggc gaaaggcaat
      301 ttgacaggag tttttgaata ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct
      361 gtagcgttat cccgatattg cggttttgct attaaaactt tcgcacccat ttaccgttta
      421 tcccttgtac atattccccc ggtttggccc gagctacgag cttttgcccg gcgagtttcg
      481 cgatatcatc tacagacaca ttattttgct ttgcccgttg ttggtaactc gctttacgtt
      541 cggcattaat ttcggttact aatttctctg tttcagcatc cgttttcagc gcaaccagat
      601 aaccataaaa tgtttcacct acccgcccct gagttctggc ttcatccaga gtcaatgcca
      661 ttacattgct gcttaccagc ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca
      721 tcgcctcaga aaagatcgct acgagtttca agcagctctt cgacatcttt gtctgccttg
      781 atgatgatct catgctcaat tttaacgttc atattgatag tgatcggttc cttaggtgca
      841 gcgacttcaa tgcgaggcgt acagccaacc agcataaaag atgacgtcaa cgcagccagt
      901 aaaattttca ttgtttttcc tcacactctt ttccttgcgg acagtcattt cccggcagac
      961 gtgcgttctg ctccaaccat gcctggagat tatcgccaaa gcgtaaactg cgccacagcg
     1021 taaaaatatt ctcttcatga tggtaattaa ggtttaccgt accgctttta ccatcaacgc
     1081 gactggtacc tgtaatgttg gcctgcatgg ttaataaacc gagattatct aagttaattt
     1141 ttgtcgatga ccggctaatt tccatatagc gcaaccagtt aattgctgaa cccgcagtca
     1201 tattgtcttt caccaccgca tctgcggtat ctttatccag ccgcaaggtc attagcccgc
     1261 tatttgccag ccaaccatct ttcactatcc atttttcgtt gtttagccat aacggcaatg
     1321 caccactaaa tgccccggac atggcgaatt gtttcggatt gacggcgcta accagttcgc
     1381 tggatgagag attattcagc cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt
     1441 tcatcagcac gttaccgcct aacacatcga cgctaacatc agtcaacagc aagggttctt
     1501 cttcagtcca cggataacgc ccttgcaaat cagccgtaat atttttcgct gtcaccagat
     1561 taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt ccccagatgc caggctccat
     1621 cggcaaaacg gaaaggcagg acaaaatcga caccgttaac ctgattatct ggcatccagg
     1681 cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa tccttgttca ggcgcagcag
     1741 aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt catcttccag tcgggtggca
     1801 ccagcggctg gaatacagtc agtgattgtt ttggccacca ggcgttaccg cgcagacgaa
     1861 taccgtccca gcgaccattt acccgaaccg ggccaatctc accagcatgt aaatcgcctt
     1921 taaagaggaa ataggtcgga ttgcgcccat cgacgctaaa ttttaaggtt gatggcggta
     1981 acacactgcc gccagtgaac agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg
     2041 agggatgctg tgcgtcacgt acccagacga cgggcttgtc gagaattaat cgcggctttt
     2101 ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt ggagagatcg gtcagcgtaa
     2161 tcgtgctgtc atgccactca ccttttcctg cgacatccca ggtggcattc atcggtgtaa
     2221 aactcccttt tccccagtag agccactgcc agcggccagc gtcagggaga aaatcattcg
     2281 ccagcccatc catatgcagc acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg
     2341 cctgcaaacg tccgtcaaca ccacgttggg tgacttttac acccgctaaa ggccagcgga
     2401 tttcatcgat gtccagcgaa tcgatgactc ttccctttga acgaagtaac gcgcctggct
     2461 caaaggtcag cgttgggtca gacagacttc cacttagctg cgcaggtaaa cgggcatata
     2521 aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag aggcagttga ctgttatcca
     2581 tgcttaattt tcctgggcca aaattaagca ccgcgttgcc cttacccgct tgcccctggg
     2641 tcagcacatt cagtcgtccg ctgaccagag cgttctcaag ccctgcctgc cagttgtcga
     2701 ctttgacacc cagtcggcca ctcaaaggaa aaccagcata cagccagctc cagcgaccat
     2761 cgcttacggt caattgttga cgagtaattt gccacggcaa atcgagcaac ggatcgccgt
     2821 tatcccgtgc cagcacgatc aattgcccgc tattttcctg ccagtccagc tcggcatcca
     2881 ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc atgcccactt acaggaagtc
     2941 catccggcac gagcggcata gtaaattccc ccaccagttt aaccggcggc tgattttcaa
     3001 acgcgacgac atccagttcg ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt
     3061 taactttttc gccctgataa cgcagttgct ggatatcaga ggttaatgcg agagagagtt
     3121 ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc gatattgatc caggtgttag
     3181 gcagcatgga ctgccactgc gcgagggttt ttggagcagc tggcgattgc tccgtctgcg
     3241 gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc
     3301 tgggatgtga aaggctggcg ttggtgatat gcgcaagctg acaatctccc accagataac
     3361 ggagatcggg aatgattaaa cctttacgcg taatgcgtgg gctttcatct aatgcaatac
     3421 gtgtcccgag cggtagccag atgtccgcca gcgtgggaac ccacagcccg agcgtcatca
     3481 gcagcgtcaa cggcacaaga ataatcagta ataacagcgc gagaacggct ttatatttac
     3541 ccagcatggg tagttaatat cctgatttag cgaaaaatta agcattcaat acgggtattg
     3601 tggcatgttt aaccgttcag ttgaaggttg cgcctacact aagcatagtt gttgatgaat
     3661 ttttcaatat cgccatggct ttcaattata tttgaaattt tgtaaaatat ttttagtagc
     3721 ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa
     3781 acagtacgac aagaagtacc tgcaacaggt gaacgagtcc tttggctttg agctggaatt
     3841 ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg
     3901 tattttcgta aacgatgacg gcagccaccc ggtgctggaa gagctgaaaa agcacggcgt
     3961 taaatatatc gccctgcgct gtgccggttt caataacgtc gaccttgatg cggcaaaaga
     4021 actggggctg aaagtagtcc gtgttccagc ctatgatcca gaggccgttg ctgaacacgc
     4081 catcggtatg atgatgacgc tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga
     4141 cgctaacttc tctctggaag gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt
     4201 tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg
     4261 tctgctggcg ttcgatccgt atccaagtgc agcggcgctg gaactcggtg tggagtatgt
     4321 cgatctgcca accctgttct ctgaatcaga cgttatctct ctgcactgcc cgctgacacc
     4381 ggaaaactac catctgttga acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat
     4441 cgtcaatacc agtcgcggtg cattgattga ttctcaggcg gcaattgaag cgctgaaaaa
     4501 tcagaaaatt ggttcgttgg gtatggacgt ctatgagaac gaacgcgatc tgttctttga
     4561 agataaatcc aacgacgtga tccaggatga cgtattccgt cgcctgtctg cctgccacaa
     4621 cgtgctgttt accgggcacc aggcattcct gacagcagaa gctctgacca gtatttctca
     4681 gactacgctg caaaacttaa gcaatctgga aaaaggtgaa acctgcccga acgaactggt
     4741 ttaatcttgc agctcccctg caatccaggg gagctgattc agataatccc caatgacctt
     4801 tcattctcta ttcttaaaat agtcctgagt cagaaactgt aattgagaac cacaatgaag
     4861 aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa
     4921 attgctgtta cgccagaaca gctacagcat catcgctttg tgctggaaag cgtaaacggt
     4981 aagcccgtga ccagcgataa aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt
     5041 tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca
     5101 gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat
     5161 aaca
//
LOCUS       DAETQL010000185         5149 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.189, whole genome shotgun sequence.
ACCESSION   DAETQL010000185 DAETQL010000000
VERSION     DAETQL010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5149
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.189"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            189..446
                     /locus_tag="K0W65_004084"
                     /pseudo
     CDS             189..446
                     /locus_tag="K0W65_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(496..1446)
                     /gene="uspE"
                     /locus_tag="K0W65_004085"
     CDS             complement(496..1446)
                     /gene="uspE"
                     /locus_tag="K0W65_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBI3097114.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="K0W65_004086"
     CDS             complement(1598..2350)
                     /gene="fnr"
                     /locus_tag="K0W65_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HBI3097115.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="K0W65_004087"
     CDS             complement(2545..3060)
                     /gene="ogt"
                     /locus_tag="K0W65_004087"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBI3097116.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3071..3214)
                     /locus_tag="K0W65_004088"
                     /pseudo
     CDS             complement(3071..3214)
                     /locus_tag="K0W65_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3604..4419)
                     /locus_tag="K0W65_004089"
     CDS             complement(3604..4419)
                     /locus_tag="K0W65_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3097117.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4516..>5149
                     /locus_tag="K0W65_004090"
     CDS             4516..>5149
                     /locus_tag="K0W65_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBI3097118.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYT"
BASE COUNT         1374 a         1175 c         1225 g         1375 t
ORIGIN      
        1 ctattttacc gcgacaattc attcagaaca tcaatagtca gggaaggaag tagcaacatt
       61 agctaactaa tcaatgcgtt gattgtaaat ccagctaaga ggtgaggttt tcagagcaga
      121 caacggtgaa acgtcatggt attgttacgt ttaggtaaca agaaatttgt ctgcacaagg
      181 attacatcat gattatggcg aaactgaagt cagcgaaagg gaagaaattt ctctttggtt
      241 tgttggcggt tttcattatt gcggcgtcgg ttgtgactcg cgcgaccatc ggcggcgtta
      301 tagaacagta caatattccg ctgtctgagt ggacgacatc aatgtatgtg atttagtcat
      361 cgatgatttt tgtttatagc ctggtcttta ctgtgttgct ggcaatcccg ttgggaattt
      421 atttccttgg cggcgaagag cagtaagtaa aaaataggcc cgataactcg ggccttgtca
      481 gttattgaag agtcgttaat cgtcttcttc gtcatccagt tcaacgggtg tctgatactg
      541 gtcaggttta atgaccagca ggtcgcagcg aagatgatca atcacctgtt ccgccgtgtt
      601 gccgaggaat gctgctgaaa taccggtgcg tccgaccgtg cccagaacca caattcccgc
      661 ctgtaaatgc tccgccaaat caggaatcac ctcttcgggc agaccttttt ctacgtgcgt
      721 catgttttca ttaatgccga atttctgccg cagggctttc attgccagca aatgttgccc
      781 acgaatggca tcgttataaa cgctcgggtc aaattccggc agttcaatcg cgatattaat
      841 tggcgttacc ggataagcgc caaccagatg aacttcggta tggttgactt gttctgccag
      901 ttcgatcgtc tctttgacca gtttttcatt gagcgcatta tgatacggct cttcactggc
      961 gagattcacc gccaccagcg ccttgcctcc ttccggccac ggctggtctt tcaccatcca
     1021 caccgggctt gggcatttgc gtaacagatg ccagtccgtt ggcgtaaaaa tcaccgcttc
     1081 cagacggtca tgttggtgcg ccatttttag caccaaatcg tgtccgccgc tgatcacttc
     1141 ctgaatgatg gcttcgaaag gacggttatg ccagaccact ttaatttcaa tgggaacgcc
     1201 agcattgaga taatattttg cctgctcgtg gatccaggct gtacgctggc tgatgacgcc
     1261 ctgacgcata gcggtacgtt cgtccgggga gagcagggtg gtcatttcgt atgagaagtc
     1321 atagatcggc aaaaaggctt taattttgcc accaatccgt tgatgtaaat aaacagctcg
     1381 ccgcaatgct ggttggtcgt cctggttagg atcgataaca acgagcatgt tctgatacat
     1441 agccatacag ggtctcctta caacaactgt caacgcagtt tgtaattaaa agattaaccc
     1501 atatctggtg aatgaaacag tgatgaacct tctgccagat caataaatca gaaaaattta
     1561 atgatatgac agaaggatag tgagttatgc ggaaaaatca ggcaacgtta cgcgtatggc
     1621 cagcaagctg ggccagcgca tcgttatttt cgatggtgat gtatttacct ttgactgcca
     1681 gcataccgct tttctggaag cgacccagca gacggctgat ggtttctacc gtcaggccca
     1741 gatagttacc aatatcgcca cgagtcatcg tcaggcggaa ttcacgaggg gagaagccgc
     1801 gttgggcaaa acgacgggac aggttgtaga tgaatgcagc cagacgttcc tcggcatttt
     1861 tcttcgacaa cagcaagatc atgtcctgat cgcctttgat ttcaccgctc atcagacgca
     1921 tcatctgctg acgcagattc ggcattttac cggacaaatc gtccagcgtt tcgaacggaa
     1981 tttcacatac catcgaggtt tccagcgcct gagcgaagct cgggtgatgg ccgctgccga
     2041 tggcgtcaaa tcccactagg tcgcctgcta aatggaaacc agtgatttgc tcgtcgcctt
     2101 gctcagtgat tgtataactt ttaatcgtac cggagcggat ggcataaagc gatttaagtt
     2161 catcaccagc cttaaacagc gtctggcctt tctgaatagg cttcttccgc tcaatgatat
     2221 tatcaagctg atcaagctca tgttcgttga gtgtgaacgg gatgcaaagc tggctgatgc
     2281 tgcaatcctg gcaatggata gcacaaccgc cagactgaat gcgccgtata attcgctttt
     2341 ccgggatcat aggtctgctc aagccgtaat tgatatttgt caattttaac atctttttag
     2401 ggagcaagta agtctaagca aaccttaaca gcagagaatt ccgatattag atgtaaatat
     2461 atgtctatct atttgaaact ccttaagttg tcaagggtaa ctttacataa aagtgtgaac
     2521 aagctggcac aaattgttta atgtttacag caaaagataa ccttcgtggc gcaataacca
     2581 ctcttttcgc tgaactccgc ctgcatatcc ggtcatggtg ccgtttcggc caataacccg
     2641 atggcaaggt acgacgatgc tgatgggatt cgatccgttt gccgcaccaa cggcacgcgc
     2701 cgcgccaggg cggcccaatt gttcagccag ttggccgtaa tgcattacct gcccgcaggg
     2761 gatagtgcgt agtgttttcc agacttcgcg ctgaaatggc gtcccccccg tggcagtggg
     2821 aagcgtatca ataatgctaa gattaccggc aaaatattca cgaagcttgt cgcttaaacc
     2881 acctgggttg gtggcagaaa tgcgctcata gccttctttg cgataatgga tgtccagcag
     2941 ctgcaccatg cgttcgctgt actcttccca ttcaaccgcc cgcaggcgaa attgctcatc
     3001 gcaaatcacc cacagtggac ccagtggcgt ggcaattttt tcttcaagta atctcagcat
     3061 ccgttctctc ttaagacaaa cgtgggtaaa taccctgacc tatcggcaga ccgacaagat
     3121 accacgccag caacatcagc agccatacca ccaaaaagat aagtggatag ggcaagacta
     3181 acgaatagta agtacccagt ttcgcgtctg gtttgtcgca gtctgatagc ataaagcgat
     3241 tttttaattt ttctgcctga agaaatgagg gcaagccatc acatggatcg agggtgggat
     3301 aattccagtc agtgaaattt taccgtaggc tatctgttat ttgattctgt ggcgtgatag
     3361 ccgccagaca ccactgaact cgtcactcat tccccaatcc cggcgagaat gtggttattt
     3421 acagagctaa aaaatgaata aaaaaataga gcctgtcgac atccgccaga ctctacagta
     3481 cacacagcag tgcatcctcg tcttaaatcc ggcctggggt cctggtgacc ggtgacagtg
     3541 ttataacggt tttacccatc cgttgccaat tttcttcagt gacagatttc acgaaaatat
     3601 aagttaaatc atttgtcttg tgattgcata attatgcatc gttaatcgac gagtaaattt
     3661 accgggcgag tagcccgttc gacgcttaaa catattgccg aacgattcag ctgaggcata
     3721 gcccagacga tgggcaattt tggtcaacga atatccatca tcaagaagcg tcactgcaga
     3781 aataacgtgc atttgctgaa cccagtggcg aaatgttaat cccgtctctt taacaaataa
     3841 gcgcatgagt gtacggctac tgacgttaat tttatccgct agattatcca gtgttggcag
     3901 atacccttgg ttgtttagca aattctcaca aagctgctgt agccgctcat catttggcca
     3961 agggagttca agtgacattt tcacccccat ttttatttcc tgaagtatca gtgtctcgag
     4021 ggctgaaaaa cgtaactgtt gtttatctgt ggtactttgc gacggtggaa gttggttaat
     4081 tgcaattagt aattccctta acaggggtga aatacccgtc atgaagcaaa ctgttcccag
     4141 cgtttcactt tccgcttctg aaaccagtac ggtttttaaa agtacgttgc ctgtcgcaac
     4201 aaacttatgc ggcatttgtg ggggcagcca aagcgcgctg ctggcatgga ctagccagac
     4261 gctttctcca gcccatacac gaacgactcc ttcagccggg taaagcaact gggcatagag
     4321 atgtttatgc cagggaactg tacctccatt cagatgttcc gaatccagtc cgataatgtg
     4381 atgccctggt gatgctttgg gttgcagtgt aattgtcatt tacagtaggt attttgtcag
     4441 aaatcatcaa ataagccatg ttaaatttta aatatgacgt atgcaagttt ccggatatat
     4501 acggtgtaaa gagtgatgat aatttctaaa aaacagctta ttggggtggt agcgatcggt
     4561 attcttttga ctggcgttgt cttttttatc tggagggtaa gcaaaggaag atttatacag
     4621 acgacggatg atgcttacat tggtggtaat atcaccacgg tggcaagtaa agtttctggt
     4681 tatatttctg ctatagaggt gcgcgataat cagagcgtaa aaaaaggcga tattatcctg
     4741 cgtctcgatg atcgggatta tcgtgcaaat gtcgccaggc ttgaggcaaa aataaaaagt
     4801 agcaaagcca atctcgaagg cattcaagcc actattacga tgcagcagtc gataattcaa
     4861 agtgccagtg aaacatggca ggcagtaaaa catgaggagc aaaagcgcct gcgagatact
     4921 gaacgttatg aaaaactcgc acaatcagcg gcaatatcgc aacaaattat tgataacgcc
     4981 aggtttgact accagcaggt cgcagcaaag gagcgcaaag ccgcgaacga tttcctggtg
     5041 gagaagcaac ggctggcagt tttatctgct caggaagaaa acgtacgtgc gagtatcgaa
     5101 gaagttcagg ccgctttgac tcaggcacta ctggatctgg aatataccc
//
LOCUS       DAETQL010000186         5114 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.190, whole genome shotgun sequence.
ACCESSION   DAETQL010000186 DAETQL010000000
VERSION     DAETQL010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.190"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(337..582)
                     /locus_tag="K0W65_004091"
     CDS             complement(337..582)
                     /locus_tag="K0W65_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097119.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1359..2201)
                     /locus_tag="K0W65_004092"
     CDS             complement(1359..2201)
                     /locus_tag="K0W65_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBI3097120.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2286..2483)
                     /locus_tag="K0W65_004093"
     CDS             complement(2286..2483)
                     /locus_tag="K0W65_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097121.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2503..2991)
                     /locus_tag="K0W65_004094"
     CDS             complement(2503..2991)
                     /locus_tag="K0W65_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097122.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(2988..3365)
                     /locus_tag="K0W65_004095"
     CDS             complement(2988..3365)
                     /locus_tag="K0W65_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HBI3097123.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3412..3789)
                     /gene="cbtA"
                     /locus_tag="K0W65_004096"
     CDS             complement(3412..3789)
                     /gene="cbtA"
                     /locus_tag="K0W65_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HBI3097124.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3869..4090)
                     /locus_tag="K0W65_004097"
     CDS             complement(3869..4090)
                     /locus_tag="K0W65_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HBI3097125.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4177..4653)
                     /locus_tag="K0W65_004098"
     CDS             complement(4177..4653)
                     /locus_tag="K0W65_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HBI3097126.1"
                     /translation="MQQLSFLPGEMTTRERSFILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(4669..>5114)
                     /locus_tag="K0W65_004099"
     CDS             complement(4669..>5114)
                     /locus_tag="K0W65_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBI3097127.1"
                     /translation="SACAPETDLQQLVATLVPDEQRISFWPQHFGLIPQWVTLEPRVF
                     GWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAG
                     IAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT         1193 a         1243 c         1355 g         1323 t
ORIGIN      
        1 gctgaggagt ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta
       61 acaaattcaa tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta
      121 atgctctaac ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct
      181 tttaatctca ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt
      241 ttgatgttga tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac
      301 tgttgcgata taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac
      361 cagaccctga ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt
      421 gtgtctgcct ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag
      481 ctgcacgttg agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca
      541 gtgcatccgc tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt
      601 gactgtcgtg ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta
      661 aattgctttc caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata
      721 tgcaatgggc tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa
      781 cgttctatct ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc
      841 ctatggatca tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat
      901 cagagtgaaa aatgttcggt ttgatgttgg gggggactga ggaggggcca acgcaggtgg
      961 tatgcgtttt acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt
     1021 agccaggtgc tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc
     1081 acagtgacca gaagggggct acttagaaga aatgttcagg ctgggtggaa gaggtaaagc
     1141 agtgacatca gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca
     1201 gcccgtaggc tggttcttct aaatagtggt gccggactcg gaatcgaaaa tttcttttat
     1261 catattgata ttaatgcaat ttgtttggtt tgattaaaaa tgtaccctca attatacccg
     1321 cagtcccttt tgatatcgaa agggaatcac tctcgggatc aggctgagag cacagaagga
     1381 aagtacctgg ctattatatc gttaattctg tcaatcagtt ccagcctttt gaaagtgatg
     1441 tgtgcagatc ccttcttaaa gtatttaatg atgaacatct cgtcttcata gcgctcttta
     1501 ccctgaacgg actggatgtg accatccaga cgaatagtga tgttttcccg gttgtcgggg
     1561 atcggtttgc cgctgaacag atgtagcatt ctttccaggt cgacgagtcg gtcagtctgt
     1621 tgcccggtga taagatgaaa cccccaccgg tcccacctca ccaggttgtt gacgataatt
     1681 ttactgccaa atttacaggg tgagttggtt ttgtaatccc agctcagccc tttgaagacg
     1741 ttgatcactc cccgttcaaa cacttcatct ttgttctggt gcagttgttc aaatgtgctc
     1801 agaatgttcg cttcgctgat ttccggaaaa ttatcatatt ccagtgacct gtaccatgtg
     1861 tcccgggtct gcgcatccat cagactcagc atcccggaac gttgcatcag atcacgccag
     1921 agttcgcgat caagattgcg tgtgatggct ttcattgcgg tttccggttt ctccatcagc
     1981 cagcaaccgc agcgaaaatc ctgtttcatg gcccagtcca gagccgtttt acctctaata
     2041 ctttctgtca ttgctgatac ttcagccagt tgtcggataa gtgtgtcgat ttgcagcaat
     2101 gtactctttc gtacagccag aatgtggcgg atactggttg agcagataac gtccgtatgc
     2161 ccgttgacca cttccggacg agaggctgtg atttcagaca taatcgatat tcctttttgc
     2221 agataaacga aaacgccagc cgtattcagg ctggcgtcag tggaatgaag tctggtgagt
     2281 aagactcacc ggttctgttg cagaaactgc agctgacgca gggtacggat atcttccctg
     2341 gcctgttcaa tacagggcag cagtgctgcc gaatccgttt tatcctcatc gttgtcgaag
     2401 ataatccctg attcgcagtc aatattgtcc tgcagccacg caatcagaat atccagcgcc
     2461 gtttctgtgg ttaataattt catccagtgt cttttgtcct gattaccagt cgaaagtggg
     2521 gaaccccggc agacattctg cgccggcgtc cagatgaatg agactgacgc cataacgccg
     2581 ggtgagagtg acgaccagac gacggaacgt tctggacagg cccaggcgtt taagccgcag
     2641 aaccgggtac gaccatgcat cggtacgtaa cagatagccg ctgcctgtaa aatgaatcca
     2701 ttctgtttcg ccgtcgtcat tagtctggtg tgacagcgag tacagccagg cgttgtccgt
     2761 ttccgtgata tgtgcggtgc tgcaacgtat gcaggtaagg gttgttaacg gtggtggtgt
     2821 actggttgcg tgttggtcgg atttatcgac cacgcggagt gaataaccgt tttcagagac
     2881 cttgttaatc agttcactga gattaacacc atcgacgtca acgacaatgc gccccatgtt
     2941 cagtgcctgc acgttaacgc tgtcggcttc cagcgtcagg gccagtttca ttgtttcgct
     3001 tccggatgct tcccccgggt aatgttattt accgttctgt agttatcgcg ggtcatcagg
     3061 ccggttgccc ggcgtgcccg gagaatatca atgctgttga ttaactgcga cggggctcct
     3121 gcgctgaatc ccggctggtc ggtacgtacc agcgcgtatt tttcaacgag aaagttcacc
     3181 gcgtcacaca gtgaaattcc tgcctcaata tgctgctcaa tcacacgttc atcagcgaac
     3241 ggtgtgtcat tcagcgtgag gccatagtgc tggtccagca gtcgggtgag cagtgtctgc
     3301 cagatggtga tgggagacgg gcagcccgat gcctcccgta cgtgagtgtc gggtaatgta
     3361 ttcatcttaa gttctctgta acaggtggtt ataaagggga gatatccggt gttatacggt
     3421 ggttgcgggc gctgccggtg tcggataaac agccagataa acgtaaccac aggagccgag
     3481 ggtgtcggct tcgcaggtca gccctttcgc atacagtgtg acggtatgct gatggcgggg
     3541 attcagttca ccgccagtga gcatgagttc cagttgtttc atcagcagcg gaaaggcctg
     3601 gtccaggtgg tacgcatcta cgtcgctgaa cctgcctctg ataccggcgc ggtctgcaag
     3661 gtaatgcagg cggttaccct cctgtaccag tcgggccccg aaacagggcg tcacggtgca
     3721 gggcagcccc caccaggggc ggtcgtgatt gtcgtcggga ggcgttgtcc cggggagtgt
     3781 gtctgacacg ataaagtcct cacagaaaat cggttaagaa tggtccggta gtggcgataa
     3841 ttctgctcat cagaattccc ttccagactc agttcagggt gacgctcatc agccggacat
     3901 acggaccaaa actgtcctta cggcgttctg caaacacagc cagtacaccg ggaatatcct
     3961 gcacttcacg gccggtatat gtatccgtgc taccgtgcca gcggtattta ccggtacaga
     4021 acggaaaaag acgggatgca ggatgctgac ggtgaatacg catggcttca ccacgggtga
     4081 tgattttcat gagaatagtc ctcctgaatg tgaaagtaag aaacagctga gaaagagaaa
     4141 ctgaagtgat atgaaggtga ttgtgacggt gacgggttaa agcagaccgt gttccgcaaa
     4201 ggagaaaacc tggctgccac cgactatcag atggtccggt acccggatat caaccagggc
     4261 cagtgcctgt accagacgct cagtgataag gcggtctgcc ttgctggggg ggacttcacc
     4321 ggacgggtga ttgtgcgcca gcaccacggc agcggcattg tggtacaggg cgcgtttaat
     4381 cacttcccgg ggatggactt ccgtacggtt gatggtgccg gtgaagaggg tttcaccggc
     4441 aatcagctga ttctggttgt tcagatacag cacccggaac tcttcacgct ccagtcccgc
     4501 catgttcaga atcagccatt cccgtgccgc atgcgtggag gtgaaggcca cgccgggttc
     4561 atgaagatgg cggtccaggg ttttcagggc ccgcagaatg aagctgcgct ctctggttgt
     4621 catttctccg ggcagaaagg aaagctgctg cattgtccct gccaccattc agtcgatgat
     4681 gcgcataatg gcgctgcatt ccggatgctg cagggcgtaa tcccgcagcc ggtaataatg
     4741 gaccgtcatg gcataacact ccgtacgaca ggcatgatga ctgtacgtca tcagacaggc
     4801 agcaatgccg gcagcttccg gactcatttc agcgcggtta ccgttcatgg cattgaacag
     4861 tacccatgtt tcgtcatcat cgtcatccgg ttcgggtgcc ataaatgccc cgccgttgtt
     4921 cagggtgtac agattccaga taccaccgca gtagtcttcg cacagacggt ccatccagcc
     4981 gaagacacgg ggctccaggg tcacccactg tggaatgagg ccaaagtgct gcggccagaa
     5041 gctgatgcgc tgttcatcag ggacgagggt ggcaaccagt tgttgcaggt cagtctccgg
     5101 tgcacaggcc gaag
//
LOCUS       DAETQL010000187         5014 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.191, whole genome shotgun sequence.
ACCESSION   DAETQL010000187 DAETQL010000000
VERSION     DAETQL010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5014
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.191"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..297
                     /locus_tag="K0W65_004100"
     CDS             <1..297
                     /locus_tag="K0W65_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBI3097128.1"
                     /translation="LFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADN
                     QQVKLSMPELEELAAAMVQAQVDRNDEIYRRQRELKEELNSLKDLNLVRNFIVE"
     gene            complement(498..1355)
                     /gene="sitD"
                     /locus_tag="K0W65_004101"
     CDS             complement(498..1355)
                     /gene="sitD"
                     /locus_tag="K0W65_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HBI3097129.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1352..2209)
                     /gene="sitC"
                     /locus_tag="K0W65_004102"
     CDS             complement(1352..2209)
                     /gene="sitC"
                     /locus_tag="K0W65_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HBI3097130.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2206..3033)
                     /gene="sitB"
                     /locus_tag="K0W65_004103"
     CDS             complement(2206..3033)
                     /gene="sitB"
                     /locus_tag="K0W65_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HBI3097131.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3033..3947)
                     /gene="sitA"
                     /locus_tag="K0W65_004104"
     CDS             complement(3033..3947)
                     /gene="sitA"
                     /locus_tag="K0W65_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HBI3097132.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4379..>5014)
                     /locus_tag="K0W65_004105"
     CDS             complement(4379..>5014)
                     /locus_tag="K0W65_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBI3097133.1"
                     /translation="PLSKTEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVD
                     FEKGIVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFY
                     HREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHT
                     FACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1323 a         1226 c         1200 g         1265 t
ORIGIN      
        1 ctgttcacgt ttgaatggaa cggtcgtatc tggaatgctg gtcccgactc actgggccgc
       61 ctgtccccgg tagtcatgct ggcaaaatct gtcacagcac aaacacatat ggcgtggagc
      121 gatgccgata atcagcaggt gaaactgtcg atgccggaac tggaagaact ggcggcagca
      181 atggtgcagg cgcaggtcga tcgcaacgat gagatttatc gccgtcagcg ggagctgaag
      241 gaagagctga atagtctgaa ggatttgaat ttggttagga attttatcgt ggaataacag
      301 aagctgcggc acgtcgtatg caggaacgtg ccgcggttgg ctggtaaact ttcgatagtg
      361 cgagtattga atgatttcta ggcgttatcg attttacgta ttttttgcat gatagaattc
      421 gtacctcctc ccaacgacct tccatggctt tccggacttc tgtaggctgg cggacgatgc
      481 tgtcctcgga tgttgtgcta attgggtgat aacagatcct gtgtatgggc gtttcccttt
      541 gtgcgtgcgt tgatgctggt aacggtaaac gtcatgatga acacgaccgc gaataagacg
      601 acaatcgtcg gagctggtgc gctgtcgata aaaaaggaca gccagacacc agtcattgac
      661 actattaccg atatgccagt cgccagtagg agcgcaatat gaaagcgttg tgtcaggagt
      721 actgcaatgg cgccaggcgc aatgagcaga gaaatagaga gaatgatacc tactgctttc
      781 agcgtcgcca caatggtcag ggaaaccatg cacagcagcc cataatgcag ccatctcgta
      841 tgcaaaccgc ttacctgcgc ctgttgataa tcaaagctga acagcaaaaa atcccgccat
      901 tttacgctaa taacaagtgt aaccagcccg gcaataatca ccgtctggat tatgtcgccg
      961 atggttatcc cgagcatgtc gccgaaaaga atatggtcaa gatgtacgtc tggtttaatt
     1021 gcaatataca agatcagacc tgcggcaaac atgccagaaa aaacaatccc cataactgta
     1081 tcttgcttga ttcggctgtt gtctttcagg tatcccgtcg ccaccgcgca gaatactccg
     1141 gcgacgaatg cccctgtagc cagaggtaac cccagaatcc aggtaagcac gatgccagga
     1201 aaaaccgcat gactcatcgc gtcacccatc agtgcccaac ctttcaggac cagaaagacc
     1261 gacagcagcg cacagggtat ggaaactacc agtgaaatca gcagtgcgtg actcataaag
     1321 gtgaactgca gtggctccag taataatgcc atcatgagcg atcctccagc gctttatgcg
     1381 cacgccgacg atttgccaaa agtccatggg tgggcgcaaa tacaaatgct aaaagaaaca
     1441 gcagtgtctg cgccaccacg atgattccac ctgtcgcgcc gtccagaaag taactgaccc
     1501 acgcgccgag gaaactggtt acactgccaa tagttacagc aattatgagc aggcggggaa
     1561 aacgatcggt taacaaccat gctgttgcac cgggagtcac caccagacag atcaccagaa
     1621 aggcaccaac cgtttgcagc gccgcaacgg tcgaaaccga cagcagggtg aagaagagga
     1681 tttttaatct gcccggatgt aatccaatag cacgggcgtg attctcgtcg aaaaaggtca
     1741 ccataagatc tttccatttg aaaaaaagca cgatgatcga gagaatgcca atgatagtga
     1801 gttgcaggat atccgccgga tcgattgcca gaatattgcc caggacgatg gtctgaatgt
     1861 tcacggatgt cgggttcaat gacaccataa acagccccag gccaaaaaag gacgagaaga
     1921 tcaggccgat gatggcatct tcttttaagc gggtgcgttg gttaagaaac agcatgctgc
     1981 ccgctgccag tccgccggaa aagaaggctc ccagagaaaa cggaagcccc agcatatatg
     2041 cccccgcaac acccgggaca atagagtgcg acagcgcatc gccaatcagc gaccagcctt
     2101 ttagcatcag atagcatgac aggaaagcgc agagaccgcc gaccattgcc gagacccaca
     2161 tcgcattgag catatattca tagctgaagg gttccagtag cacgttcatc ttgcttccct
     2221 ctgcaccgct gacggtcgat gtgccacaaa agggcgttca tcgtcagtaa taatgctttc
     2281 ttccgagccg ttaagtgtga catggcgcag tacgccgcta aaagccagct ccaggttctc
     2341 ggctgtaaaa gtggtgtctg tcggcccgct tgccaggacg gtgcctttga ccatgaccgt
     2401 ataatcgcaa aatgtcgtga ctgagccgag attgtgcgtt gagacaagca tcgttttgcc
     2461 ttccgcgcgt aactccctca acaggctgat gattttcgct tcggttttca catcaacgcc
     2521 ggtaaaaggt tcatccagca ggatcacatc accctgctgt gcaattgctc tcgccagaaa
     2581 gacgcgtttc ttttgtccac cggaaagctc gccgatttga cgatggcgaa aatccaccat
     2641 atcgacacgt tccagcgcat cggtgacaat ctgacgatcc ctctttttgg ctatacgcaa
     2701 catacccata tgcccatagc ggcccatcat caccacatct tccaccagta caggaaatga
     2761 ccagtcaacc tcttctgact gagggacgta ggcaaccagg tttttctgta gcgcctgtcg
     2821 cgtgggaata cccagaacag atatctttcc gctggtcaga cgcacaaatc ccataatcgc
     2881 tttaaacaac gtcgattttc cggaaccgtt taccccaacc agagcggcaa tagatccact
     2941 tggtactgtg aaggatgcat cgcgcagtgc tgtgtgcccg ttacgccagg tgacagtaac
     3001 atcattaacg acaatgcctg cagattgcat cattatttct cccttttccc ggctttaatt
     3061 ccctgtacca gcgtactggt agtaacctta agaaggtcga tatacgtagg taccgggccg
     3121 ttttctgtgc tcagggaatc gacatagagc acaccgccgt agtgtgcgcc ggtttcacgc
     3181 gcaacctgac gcgctggttt atcggaaatc gtactctcgc taaagactgc cgggatatga
     3241 tttttcttaa ctatatcaac aaccttacgt acctgctgcg gtgttccttg ttgatcggca
     3301 ttaatcggcc acagataaag ctcttttagc cccaaatcgc gtgcgagata agaaaaagcc
     3361 ccttcactgg tgaccatcca tcgctgattc tcagggagtt ccgtaatctg cttacgcagg
     3421 ggggcaaggg tttgggtaat cttggcttta taagtatcgg cattacgttg gtaggtttgt
     3481 gcatttgccg gatcgtattt tatcaacgca tcacgaatat tatcgacgta aatcagagca
     3541 ttatctggcg acatccatgc atggggatta ggtttgccct catagggtcc ttcggtgatc
     3601 cctactggcg tcacacccga agagacaatt acttctggaa ccccattgag atgctggtaa
     3661 aagcgttgga accacaattc cagattcata ccattggcga gaatcagttg tgccccctgc
     3721 gcacgtttaa tatcgccagg ggtaggctga tactcatgaa tttctgcacc aggcttggtt
     3781 atggatgaga cttctgcagc atctccagcc acgtttttgg ccatatccgc gatgatggta
     3841 aatgttgtaa tgaccttgaa tttttcagtt gcacttgcct gaaatgcgat cgagcaggtg
     3901 agggcgaggc accccaagag catggttact ttttttattg agtgcataat ttagtaccta
     3961 tagtgagcaa aattagaaca aacatagcaa cggctatgtt ttatagcata acctatacaa
     4021 aatgaaaata attatcatta acagctttga ggcctaaaac agggattagg ttgtttcctg
     4081 ctggcgacat gatattgagg aaaaaagtag agcagctatt aggctttgaa atgactttat
     4141 gaggaaaatt gttgatggca aaaatggggc aaaaaagagt gggttatcgt agcttattgt
     4201 tgtcgctgat aatgtttaac atattgaaaa ataaataaaa tacttatgag tcagatagtt
     4261 gtgatttttg cccttacttg ttcaggttgt attgttcttt ctttgtaatt tgttgatttt
     4321 cttgcattat ttcagttctc tggtactaaa tggggcaaat tgggggcaaa ctttgcaact
     4381 acgataaccg cgcattcaac atggctatct gttcgtcgtt catgtcatca atccacatac
     4441 cgtaaatttc atacaccatt tgcgcagttt catgccccat ctggctggct ataaatgccg
     4501 ggttcgctcc tgccgtcaac agccagcagg caaaagtatg tcgcgtatgg tacggattac
     4561 ggcggcgaat accagcacgt tttactgctg cattccatct tgtacccaaa ctgcttaccg
     4621 agtaataagg tttctgtttt tcgttacaca ccctgggcat gaaaacaaaa tgcagttttt
     4681 gtttttcagt tctgccgtac tcccgatgat aaaaagtgat ttcgcttttg cgatgatgcc
     4741 cggtcagttt gtattgctcc ttcagtgctt caagagccgg ctgcagtaat gctaccgtcc
     4801 ggatcccggc atttgttttt gggggaccga acatatcaag tatcgtcagg tttcttctga
     4861 cattcacaat tcccttctcg aaatccacat cctcccacgc cagagctgcc agttccccgt
     4921 gacgaagccc ggagtaaacg gcaaatttcc acaagttttg gctctgtcct ttttcacttt
     4981 ccattaatgc attgaattct gttttagata acgg
//
LOCUS       DAETQL010000188         4972 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.192, whole genome shotgun sequence.
ACCESSION   DAETQL010000188 DAETQL010000000
VERSION     DAETQL010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.192"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(186..770)
                     /gene="kptA"
                     /locus_tag="K0W65_004106"
     CDS             complement(186..770)
                     /gene="kptA"
                     /locus_tag="K0W65_004106"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HBI3097134.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            1019..1702
                     /locus_tag="K0W65_004107"
     CDS             1019..1702
                     /locus_tag="K0W65_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HBI3097135.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1699..2160
                     /locus_tag="K0W65_004108"
     CDS             1699..2160
                     /locus_tag="K0W65_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HBI3097136.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2173..3345
                     /gene="iadA"
                     /locus_tag="K0W65_004109"
     CDS             2173..3345
                     /gene="iadA"
                     /locus_tag="K0W65_004109"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBI3097137.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3410..4321
                     /gene="hypT"
                     /locus_tag="K0W65_004110"
                     /gene_synonym="qseD"
     CDS             3410..4321
                     /gene="hypT"
                     /locus_tag="K0W65_004110"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBI3097138.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4314..4706)
                     /gene="iraD"
                     /locus_tag="K0W65_004111"
     CDS             complement(4314..4706)
                     /gene="iraD"
                     /locus_tag="K0W65_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HBI3097139.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4703..4786)
                     /gene="idlP"
                     /locus_tag="K0W65_004112"
     CDS             complement(4703..4786)
                     /gene="idlP"
                     /locus_tag="K0W65_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HBI3097140.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1176 a         1305 c         1239 g         1252 t
ORIGIN      
        1 gtatgaccgg aagcgacgct gattccagtc cccaactgac cgcctgccgt aaatcacttt
       61 aatttcagca gttgttatta aaggataccc ttttacgagg gtatcccttc agactgataa
      121 caaacgctac attgccggat gtgctgcgct taccaggcct atataacccc tgcaatatat
      181 tgaatttatt gtttttgtac cccagtcggc ataaacgacc actcaagaaa ctcaacagca
      241 accgttgatg tcagccagac cccgttttcc gcttgccaga aagggatgcc tcgtttcgcc
      301 atttcctgtg ctttgacggt taagacaacc ggcgatccgt gtcttgctcc cactttgcgt
      361 gccgttgctt catcagcaga gagatgcacg taatgacgat cccctgcaat cagcccctgt
      421 ttttttatct cgtcaaggaa acggcttgcc gtaccgtggt agagaaactg tggcggtgtt
      481 ttttcggcaa aggaaatagc gacctgcgaa gtcgaatgcc cctgaaccgc gcggatgcac
      541 ctaccatcac tggaataact aaaacgcttt ttatcgctgg ttgcgaccac ggtatccagc
      601 aatgcacggg ttaatcgctt gcccgctttt tgggcacaaa gtatgagctt attgatatcc
      661 gcccatcctt cacggtccag tacgatacca atcgcctccg gtttgtgtcg gaggacaaaa
      721 cttaaaaact tgctcgtatc cgccagctct ttttcgttgt actttgccat cgcgttttcg
      781 tccctggatt ttctgttttt atttactaat gtcattgtca ctgggtttat gaattttctg
      841 caactcaatc ctgcgcagaa ccttttccac acagcgtaat ttttccccta tgcaaaaaac
      901 gcatagccag aaacatactt gcattttccc catacccaca tggctgataa ggttttcagt
      961 tccaggaatt ttcctggctc aagcaacaca caaacacaaa aaaacaatgg ggattgttat
     1021 gacacaacaa ggggatgctg tagccgggga gctggcgaca gaaaaagtgg gcattaaagg
     1081 gtatctggct ttttttctca ccataatctt ttttcccggt gttttttccg gaacagacag
     1141 ctggtggcgc gtttttgatt tttccgttct gaacggatcg tttgggcaac tgcctggtgc
     1201 gaatggagcc accacctcat ttcgcggggt gggtggcgcg ggggctaaag atggctttct
     1261 ctttgcgctg gagctggcac cgtcagtcat tctctctctg gggattattt caattaccga
     1321 tggtcttggc ggattacgcg ccgcgcagca gttaatgaca cctgtgctaa aaccgttgtt
     1381 gggcattcca gggatctgct cgctggcact gatcgctaac ctgcaaaaca cggatgccgc
     1441 cgccggtatg accaaagagc tggcgcagga aggtgaaatt accgaacgcg ataaagtgat
     1501 tttcgccgca tatcaaacca gcggtagcgc catcatcacc aactacttct cttccggcgt
     1561 cgcggtgttt gcctttctgg gcacgtccgt aatcgtacct ttagccgtca tcctggtgtt
     1621 taaatttgtc ggagccaaca ttttgcgcgt ctggcttaac ttcgaagaac gtcgcaaccc
     1681 aacgcaagga gcgcaagcat gacaactcag gtccgcaaaa acgtcatgga tatgtttatc
     1741 gatggcgcgc gccgtggctt tactatcgcc acgaccaacc tgctgccaaa cgtggtgatg
     1801 gcgtttgtca tcattcaggc gctgaaaatt accggcctgc tcgactgggt ggggcatatc
     1861 tgtgaacccg taatggcgct gtggggatta cccggtgaag cggcaaccgt ccttttagcc
     1921 gcattaatga gtatgggcgg cgcagtcggt gttgccgcca gtctggcaac cgctgacgca
     1981 ttaaccggcc acgatgtcac cgttttactg cccgcaatgt atttaatggg caacccggtg
     2041 caaaatgtcg gtcgctgcct ggggacagcc gaagtgaatg ccaaatatta cccgcatatc
     2101 attacggtct gcgtgattaa cgcattgctg tcgatctggg ttatgcagct tattgtttaa
     2161 caaggagtta ccatgattga ttataccgca gccggtttta ccctgctgca gggagcgcat
     2221 ttgtatgcgc cggaagatcg gggaatttgc gatgtcctcg tcgctaacgg caaaattatc
     2281 gccgttgcca gcaatatccc ttctgacatt gtaccgaact gcacggttgt cgatctcagc
     2341 gggcagatcc tctgcccagg ttttattgat caacacgtcc atttgattgg cggtggcggc
     2401 gaagcaggtc ccacgacgcg cacgccggaa gtggcgctaa gtcgcctgac ggaagcgggc
     2461 gtcacgtcag tggttggtct gctgggcacc gactctatct ctcgccaccc ggaatccctg
     2521 ctcgccaaga cccgtgcgct caatgaagaa ggcatcagcg cctggatgct gaccggcgct
     2581 tatcatgtcc cttcccgcac tattacgggt tccgtggaaa aagacgtggc gattatcgat
     2641 cgtgtgattg gcgtgaaatg cgccatctct gatcaccgtt ctgccgcacc ggacgtttat
     2701 cacctggcta atatggcggc agaatcccgc gttggcggtt tgctcggcgg taaacctggc
     2761 gtcaccgtgt tccacatggg cgacagtaaa aaggcgttaa agcctattta tgacctgctg
     2821 gaaaactgcg atgtgccgat cagcaagctg ctgccgaccc acgttaaccg caacgtaccg
     2881 ttgtttgagc aggcgctgga gttcgcgcgc aaaggcggca ccatcgatat caccagcagc
     2941 attgacgaac cggtcgcccc tgccgaaggt attgcccgcg ccgttcaggc gggtattccg
     3001 ctggcacgcg tcaccctcag ctccgacggc aacggtagcc agccgttctt cgatgacgaa
     3061 gggaatttaa cccatatcgg tgttgcaggt tttgaaacgt tgctggaaac cgtgcaggtg
     3121 ctggtcaaag actatgattt cagtatcagc gatgccctgc gcccgctcac cagtagcgta
     3181 gccggtttcc ttaacatgag cgggaaaggc gaaattctgc caggcaatga cgcagactta
     3241 ctggtcatga cgccagaatt gcgcattgag caggtatacg ctcgcggcaa actgatggtc
     3301 aaagacggca aagcctgcgt gaaaggaacg tttgaaacgg cttaaacggt tgtcagccgc
     3361 cctaatggac gtagaatgcc cccaagggcg gctgacagag taaaacgtaa tggatgactg
     3421 tggtgcggtt ttgcataata ttgaaaccaa atggctttat gattttctga ctctggaaaa
     3481 atgccgcaat ttttcccagg cggcagtcag tcgcaacgtc tcgcaaccgg cattcagccg
     3541 ccgcatccgt gcactggaac aggcgattgg cgttgaattg tttaaccgcc aggtgacgcc
     3601 actgcaactc tcagaacaag ggaaaatctt ccattcgcag atccgccatc tgttgcaaca
     3661 gttagaaagc aaccttgcag agctgcgtgg cggcagcgat tacgcgcaac gtaaaatcaa
     3721 gatagccgct gcacactctc tttccctcgg tctgttaccg tccattatca gccagatgcc
     3781 gccgctcttt acctgggcaa ttgaagccat tgatgtcgat gaagcggtcg ataaactgcg
     3841 tgaagggcaa agtgactgta ttttttcctt tcacgacgaa gatttgctgg aagcgccgtt
     3901 tgaccacatt cgcttatttg aatctcaatt gttccctgtc tgcgccagtg acgaacacgg
     3961 agaagcactt tttaacctcg cgcagccaca ctttccgtta ctgaattaca gccgcaactc
     4021 ctacatggtg cgattgatta atcgcaccct gacgcgccac agtgagttaa gtttcagcac
     4081 cttttttgtc tcttcgatga gcgagctttt aaagcaggtt gccctcgacg gctgtgggat
     4141 tgcctggctg ccggagtatg ccatccaaca agaaattcgc agcgggcagc tcgttgtgct
     4201 gaatcgggac gaacaggtga tcccgattca ggcctacgca taccggatga atacccgcat
     4261 gaatccagtt gccgaacgct tctggcgtga actgcgcgag ctggagattg tgcttagctg
     4321 acattctcaa gcgtcgcact gcgccaaccg ccattatccc agaccagatc aaacgttagc
     4381 ggttcatcgt cgtggtacag cattatctca aggctgatcg ccagagtgtg gcagtacgct
     4441 tctttcaaat taacctgcaa cgccgcaatc cgtggctcat gaaataacag tgcagaacgc
     4501 agctgacggc aataccagct ctggagatgt ttgtcgctcg ggttatgcac ttgccactcg
     4561 ggcatgcctg aaacaagtaa tgacggtaat gccgaatgag gagaattaaa aagagtgagg
     4621 agatcgctgc ataccgattt acgcagcaat gacgttttat tgcctgaaat ttcaggtaaa
     4681 acagcctgaa gtgattggcg catcattttg cgcactcctg acgtttagca acatatgcgg
     4741 cttgccctga acaccgggca ccactgtatt gatgctcatt ttccatagcc attccctttt
     4801 gttcattaca tccatgtcag tctgagacag gcctgaatcc tgtcagcaaa gtataaaacc
     4861 ttacttgcct atgaatatct actgactttt caggaaaaaa gtcatgattt gataaaaatc
     4921 atcaaactca aaaaaaaaac gctagtatat tctgaatatt ccgaatagtt ac
//
LOCUS       DAETQL010000189         4873 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.193, whole genome shotgun sequence.
ACCESSION   DAETQL010000189 DAETQL010000000
VERSION     DAETQL010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.193"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..530
                     /locus_tag="K0W65_004113"
     CDS             <1..530
                     /locus_tag="K0W65_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBI3097141.1"
                     /translation="RTTGRAEATILVDIAHQFWHIPHEKIWIEDQSTNCGENARFSIT
                     LLNQAVERVHTAIVVQDPTMQRRTMATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSV
                     IFINQLQGLWPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLK
                     HDAVLIEAMESRSLR"
     gene            727..2166
                     /gene="aldA"
                     /locus_tag="K0W65_004114"
     CDS             727..2166
                     /gene="aldA"
                     /locus_tag="K0W65_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBI3097142.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(2208..3208)
                     /gene="gap"
                     /locus_tag="K0W65_004115"
                     /pseudo
     CDS             complement(2208..3208)
                     /gene="gap"
                     /locus_tag="K0W65_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3397..3927
                     /gene="cybB"
                     /locus_tag="K0W65_004116"
     CDS             3397..3927
                     /gene="cybB"
                     /locus_tag="K0W65_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBI3097143.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            4172..4345
                     /locus_tag="K0W65_004117"
     CDS             4172..4345
                     /locus_tag="K0W65_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097144.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(4417..4566)
                     /gene="hokB"
                     /locus_tag="K0W65_004118"
     CDS             complement(4417..4566)
                     /gene="hokB"
                     /locus_tag="K0W65_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBI3097145.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(4667..4807)
                     /locus_tag="K0W65_004119"
                     /pseudo
     CDS             complement(4667..4807)
                     /locus_tag="K0W65_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1247 a         1122 c         1208 g         1296 t
ORIGIN      
        1 tccgcaccac tggcagagca gaagcgacca tcctggtgga tatcgctcat cagttctggc
       61 atattccgca tgaaaaaatc tggattgaag accagtcaac aaactgcggt gaaaacgcac
      121 gctttagcat cacgctattg aatcaggccg tagaacgagt tcatacggct atcgttgtac
      181 aggaccccac catgcagcgg cgcacgatgg caacgttccg ccgtatgacc ggggacaatc
      241 ccgatgcacc acgctggtta agttatcccg gattcgttcc tcagttagga aataacgcag
      301 acagtgtaat ctttattaat cagttacaag gattatggcc ggttgagcgt tatctctcac
      361 tactcactgg cgagctgccg cgtttacacg atgatagcga tggctacggt ccccgcgggc
      421 gagattttat cgttcacgtt gattttccgg cagaagtcat ccatgcatgg caaacgctga
      481 aacatgatgc ggtgctcatc gaggcgatgg aaagtcgctc gttacgttaa aaattgcccg
      541 tttgtgaacc acttgtttgc aaacgggcat gactcctgac ttttatttct gccttttatt
      601 ccttttacac ttgtttttat gaagccctcc acagacttgt cctttcccga tgccgtctct
      661 ctgatgattg atgttaatta acaatatatt caccgaaaac aaacatataa atcacaagag
      721 tcgcccatgt cagtacccgt tcaacatcct atgtatatcg atggacagtt tgttacctgg
      781 cgtggagacg catggattga tgtggtaaac cctgctacag aggctgtcat ttcccgcata
      841 cccgatggtc aggccgagga tgcccgtaag gcaatcgatg cagcagaacg tgcacaacca
      901 gaatgggaag cgttgcctgc tattgaacgc gccagttggt tgtgcaaaat ctccgccggg
      961 atccgcgaac gcgccagtga aatcagtgcg ctgattgttg aagaaggggg caagatccag
     1021 cagctagctg aagtcgaagt ggcttttact gccgactata tcgattacat ggcggagtgg
     1081 gcacggcgtt acgagggcga gattattcaa agcgatcgtc cgggagaaaa tattcttctg
     1141 tttaaacgtg cgcttggtgt gactaccggc attctgccgt ggaactttcc gttcttcctc
     1201 attgcccgca aaatggctcc cgctcttttg accggtaata ccatcgtcat taaacccagt
     1261 gaatttacgc caaacaatgc gattgcattc gccaaaatcg tcgatgaaat aggccttccg
     1321 cgcggcgtgt ttaaccttgt actggggcgt ggtgaaaccg ttgggcaaga actggcgggt
     1381 aacccaaagg tcgcaatggt cagtatgaca ggcagcgtct ctgcaggtga gaagatcatg
     1441 gcgactgcgg cgaaaaacat caccaaagtg tgtctggaat tgggtggtaa agcaccggct
     1501 atcgtaatgg acgatgccga tcttgaactg gcagtcaaag ccatcgttga ttcacgcgtc
     1561 attaatagtg ggcaagtgtg taactgtgca gaacgtgttt atgtacagaa aggcatttat
     1621 gatcagttcg tcaatctgct gggtgaagcg ttgcaggcgg ttcaatttgg taaccccgct
     1681 gaacgcaacg acattgcgat ggggccgttg attaacgccg cggcgctgga aagggttgag
     1741 caaaaagtgg cgcgcgcagt agaagaaggg gcgagagtgg cgttgggtgg caaagcggta
     1801 gaggggaaag gatattatta tccgccgaca ttgctgctgg atgttcgcca ggaaatgtcg
     1861 attatgcatg aggaaacctt tggcccggtg ctgccagttg tcgcatttga cacgctggaa
     1921 gatgctatct caatggctaa tgacagtgat tacggcctga cctcatcaat ctatacccaa
     1981 aatctgaacg tcgcgatgaa agccattaaa gggctgaagt ttggtgaaac ttacatcaac
     2041 cgtgaaaact tcgaagctat gcaaggtttc cacgccggat ggcgtaaatc cggtattggc
     2101 ggcgcagatg gtaaacatgg cctgcatgaa tatctgcaga cccaggtggt ttatttacag
     2161 tcttaatgag tgaaagaggc ggaggttttt tcctccgcct gtgcgcgtca gagtttagcg
     2221 aatttttcga gggtgcgaat aagctgcgtg acgaagccat attcgttatc gtaccaggcg
     2281 accgttttca ccagttgtaa atcgcccacg gcggtaattt ccgtttgcgt ggcatcaaac
     2341 actgaaccga aatggctgcc aatgatatcg gaagagacta tttcttcatc ggtataacca
     2401 aatgactcgt tattggtggt tgcttgttta agtgcgttat tcacctcttc ggcagtcact
     2461 tttttccgag aatggacacc agctcagtga ccgaacctgt tttcaccggc acgcgttgcg
     2521 catgaccttt cagtttgccg ctcagttccg ggatcaccag accaatggct tttgccgccc
     2581 ccgtagtgtg gggaatgata ttttctgccg ctgcgcgtga agcacgtaaa tctttaccac
     2641 gcgggccatc caccagtgac tgggtgccag tataggcatg aatggtcgtc atcgtgccga
     2701 cttctatccc gaaactgtca tgcaaggctt tggccatcgg cgcaaggcag ttagtggtgc
     2761 atgacgccac ggaaacaatg gtgtcgttgc catccagagt gtcgtcatta acgttataaa
     2821 cgatggtttt catttcaccg gcaggagcgg aaatcaacac cttcttcgca ccagcatcaa
     2881 gatgcgcctg cgatttatcg gcggaggtat aaaagccagt acattcgaca atgatttctg
     2941 cccctttcgc tttccacgga atatttttag cctctttttc ggcgtaaacc gcgatacttt
     3001 tcccatcaac gataagtgaa tcttccgtaa aatcaacgct ccaggggaac ggaccatagt
     3061 ttgaatcatg tttcagcaga taggcgagaa tttttgggga agtaagatca ttaatagcga
     3121 caacgtctat gttgcttttg acttcaagta aacgacgcaa caccagtcga ccgatacgac
     3181 caaaaccgtt aataccaact ttactcatgg ttttctcctg tcaggaacgt tcggatgaaa
     3241 attgatcctt tccaggctta gaccaggatt gtgggatggg caatctccat tcttacagtg
     3301 aaacgtaacg tactgaaaac gggtgaacaa tatttaatga aattttgaga aaagcccgtt
     3361 atgttaacgg aaaattatgt taaagcagga aatgttatgg aaaataaata ctcaaggtta
     3421 caaatcagca ttcactggct ggtctttttg ctggttatcg cagcgtattg cgcaatggag
     3481 tttcgtggtt tcttcccacg tagcgatcgg ccgctcatca acatgattca tgtttcctgt
     3541 ggcatctcaa ttctcgtgct gatggtggtt cgtcttttgt taagactgaa atacccaacc
     3601 ccgccgatta tacctaagcc gaaaccgatg atgacgggac tggcgcattt gggacatttg
     3661 gtgatttatc tcctgtttat tgcgctgcca gtgattggtt tggtgatgat gtataaccgg
     3721 ggcaacccgt ggtttgcgtt tggtttgacg atgccttacg cttcagaggc caatttcgaa
     3781 cgggttgata gcttaaagtc gtggcatgag acgctggcga atctgggata ttttgtcatc
     3841 gggttgcacg ctgcggctgc actggcacac cactatttct ggaaggacaa cacacttcta
     3901 cgcatgatgc cgcgtaaacg ttcctgaagg atatttaaag aaaacgcctg tactaaaacc
     3961 gacccgtggt acaggcgaag aatacgggtc tacatcggaa gcgcctatta tatttatttg
     4021 tatgacaaat aaaaccataa tccttgccca tacgtccatc tggcttattt ttaatcaatt
     4081 cacccgatct ttgatctcat caacggtatc aaaataaacc agcgtataac gttcaaatcg
     4141 cacataagat tcgataaacc atggaggtta tatgaaaaaa ctggcactta ttttgtttat
     4201 gggaacgctt gtttcctttt atgccgatgc cgggcgcaaa ccctgttctg gttcgaaagg
     4261 ggggatctca cactgtacgg caggcggcaa atttgtctgt aatgatggtt ctattagtgc
     4321 atcgaaaaaa acatgcacta actgaagtgt aaaaggggtg ccatgagaaa atgaatctgc
     4381 tagtcagatg cgcggggaaa atccccgcgc ttgcccttac ctggacgtgc aggccatgag
     4441 cgcagcaacc tccttatcac cgtcccggaa ccgcagttcg tagagcgttt gtcgggtcag
     4501 gagtgtgaat gccagaatcg taatgcagat aatgagcaga cacaccacca gagggttgcg
     4561 cttcatagcc tttctccttg ccggatggcg ggtaagaggc taacaatctg aattgcttgg
     4621 ttcattcgtt ggcctcggtt tgtactgacc ccaaaaagtt ggacagttaa acacgaggca
     4681 tataggtctg attccgatat tcatttggag tcagaccttt taatttcagg ctaattcttc
     4741 tgctgttgta gtattcaata tattccgtaa cagcatcctt cagttcgctt atattactga
     4801 actcattaga ctggccccct gaatctccag acaaccagta tcacttattt aagtgatagt
     4861 cttaatacta gtt
//
LOCUS       DAETQL010000190         4638 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.194, whole genome shotgun sequence.
ACCESSION   DAETQL010000190 DAETQL010000000
VERSION     DAETQL010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.194"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..313
                     /locus_tag="K0W65_004120"
     CDS             <1..313
                     /locus_tag="K0W65_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBI3097146.1"
                     /translation="ALDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYC
                     QYYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASM
                     A"
     gene            470..1219
                     /locus_tag="K0W65_004121"
     CDS             470..1219
                     /locus_tag="K0W65_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HBI3097147.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1241..1807
                     /gene="yieF"
                     /locus_tag="K0W65_004122"
                     /gene_synonym="chrR"
     CDS             1241..1807
                     /gene="yieF"
                     /locus_tag="K0W65_004122"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HBI3097148.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1861..3198)
                     /gene="adeP"
                     /locus_tag="K0W65_004123"
     CDS             complement(1861..3198)
                     /gene="adeP"
                     /locus_tag="K0W65_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HBI3097149.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3363..4019
                     /gene="yieH"
                     /locus_tag="K0W65_004124"
     CDS             3363..4019
                     /gene="yieH"
                     /locus_tag="K0W65_004124"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBI3097150.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4086..4559
                     /locus_tag="K0W65_004125"
     CDS             4086..4559
                     /locus_tag="K0W65_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HBI3097151.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1239 a         1162 c         1139 g         1098 t
ORIGIN      
        1 ggcactggac aatgtgttac aggagctggg gcgcgaacgc acgattgcta tgagcctgcc
       61 ggaattcgag cagtcactgt ttatggcagc gcaacccgac aatctgctac tggcgaccgc
      121 gccgcgctac tgtcagtact acaatcaact ccatcaactg ccgttggttg ctcttcctct
      181 cccgtttgac gaaagccagc aaaaaaagct ggaagttcct tttaccctgc tgtggcataa
      241 acggaacagc cataatccga agatcgtctg gttacgggaa accattaaaa acctttacgc
      301 gtcgatggca taaccgaatc gtatgaaacg cgacccaatt tcacaataaa atgtaaaaaa
      361 gttgtaataa gcttgtctga atcgaacttt tagccgcttt agtctgtcca tcattccagt
      421 aaatgattac tcttgtattc ataatggacc attaagcatg gagcgaaaaa tggcgactca
      481 ctttgcccga gggattttaa cggaaggaca tctgatttct gttcgtctcc cctcccagtg
      541 tcatcaagaa gcccgaaaca ttccccctca tcgtcaaagc cgttttctgg cgtccagagg
      601 tttactcgca gaactgatgt tcatgctgta tggcattggc gaattgccgg aaatcgtcac
      661 cctgccgaaa ggtaaaccgg ttttcagtga taaaaatttg ccttcgtttt ctatttccta
      721 tgccgggaat atggttggcg tggcgttaac aaccgaaggt gaatgtggcc tcgatatgga
      781 actacagcgt gcgacgcgcg ggtttcatag cccacacgcg cccgataacc acaccttttc
      841 cagcaatgaa tcgctatgga tcagtaaaca aaacgatcct aacgaagcgc gggcgcagct
      901 cattacgctg cgccgaagcg tgctaaaact aaccggtgat gttttgaatg acgatccgcg
      961 cgatctgcag ctgctgccca ttgccggacg cctgaaatgt gctcatgtaa atcatgtaga
     1021 agcgttatgc gacgcggagg acgtgctggt gtggtccgtg gcggtcacgc ccgcgattga
     1081 aaagctcagt gtctgggagt tagatggcaa acacagctgg aaaagcctgc cggatattca
     1141 cagccgcgcc aacaatccta ccagccggat gatgcgtttt gcccagctct ctaccgtgaa
     1201 gtctttttcg ccaaattgat agacaaccac aggagtcatt atgtctgaaa aattgcaggt
     1261 ggttacgtta ctggggagcc tgcgcaaagg ctcatttaat ggcatggttg cacgtaccct
     1321 gccgaaaatt gctccggcga gcatggaagt caatgcgtta ccttccattg ccgacattcc
     1381 cctgtatgac gctgacgtac agcaggaaga aggttttcca gcaacggttg aagctctggc
     1441 ggaacagatc cgtcaggctg acggtgtggt gatcgtcacg ccggaatata actactcggt
     1501 accgggtggg ctgaaaaatg ccatcgactg gctttcccgc ctgccggatc aaccgctggc
     1561 cggtaaaccg gtgttgattc agaccagctc aatgggcgtg attggcggcg cgcgctgtca
     1621 gtatcacctg cgccagattc tggttttcct cgatgcaatg gtgatgaaca agccggaatt
     1681 tatgggcggc gtgattcaga acaaagttga tccgcaaacc ggagaagtga ttgatcaggg
     1741 tacgctggac cacctgaccg ggcaattgac cgcatttggt gagtttattc agcgagttaa
     1801 gatctaaata aaaaacccgc cagtaatcat gcatggcggg tttttaatgc gctatcgatt
     1861 ttaatgagcg tcgataaata caatcttcag gataaacagc agcgcaacga tgattacgca
     1921 cgggctaagg tcacgcagac gcccggtacc aatcttcatc acgcagtagg agataaagcc
     1981 cagcgcaata ccttcggtaa tcgaaaagct gaacggcatc atcacggcgg taataaacgc
     2041 cggaacagat tcagtaagat cctgccagtt cacgcgagca agacttgagg tcatcaacac
     2101 gccaacgtag atcaacgcgc cagctgcagc gtagtctggc accattcccg ccagcggcga
     2161 cagaaagata accagcagga acagcagacc aacaaccact gccgtcagac cggtacgacc
     2221 gccaaccgat acgccggaag aggactcaat ataagccgta acggaagaag taccgataaa
     2281 cgaaccggtc acggaagaga tactgtcgac atacagcgcc tgcttcatgc gcgggaattt
     2341 ccccttctca tccgccagac ctgctttatc ggtcacgcca atcagcgtac cggaggagtc
     2401 aaacaagttg accaacatga aagagaaaat cacccctgcc agcccgaggt taaacgaccc
     2461 ggctaaatct acatgaccca caactgtcat tacgctcggc ggcgcagaaa cgatgccatt
     2521 gtagtgcaca tcacccagca tccagcccag cagcgtcgtc accacgatag aaaccagcac
     2581 cgctgcgtga atgttgcgcg aggccagaat agcaatgatg aaaaagccga ggatacccag
     2641 aagtacgctg tgagaagtca gattaccgat gctcaccagc gtttccgagt tagcgacaat
     2701 cacacctgcg tttttcagcc ccatcatgcc gataaacagg ccgataccgc tggtaatacc
     2761 cacacgcaga cttaccggaa tgttagcaat catccagtaa cgaacgcgga aaatggtcaa
     2821 aagaagcagt ccgacagcgc cccagaagat tgcgcccata ccgatctgcc acggcagccc
     2881 cattgcctgg acaaccacaa aagcgaaaaa ggcgttaagc cccatcgcag gagccagcgc
     2941 caccggaagg tttgcaaaca atcccatcat aatgctaccg aaagcagcaa tcagacaggt
     3001 tgttacgaag acggcactgg tgtccatgcc agcaacgccc agaatttgtg ggttaacaaa
     3061 tacgatgtag accatcgtca ggaaggtggt gaacccggca atcacttcag ttcgtgccgt
     3121 agtaccatgt tcgcgcagtt taaaaacacg ttccagcatc ctctgaccag aagtctgggt
     3181 ggtatgttga tgactcatta tctatttccg aacaaggagg gaaaatccgt cgctatcgta
     3241 taccaaaatg cgacaatagg cgcgtttgtg agagactttt ttattggatt tacttatacg
     3301 gcaacgattg cgttgcgcaa atcggctcta cgaaaacgtt aaactgatta aaaaggaaag
     3361 gcatgtcccg gatagaagcg gtatttttcg actgcgacgg tacgctggtc gacagtgaag
     3421 tcatttgctc ccgcgcatat gtaacgatgt ttcaggaatt tggtattacg ctcgatcctg
     3481 aagaggtatt caaacgtttc aaaggtgtaa aactgtacga aattatcgat attgtttccc
     3541 ttgaacatgg tgttacgtta gcgaaaacag aagctgaaca cgtttaccgt gcagaagtcg
     3601 ctcggctgtt cgattcagcc atcgaagggg ctggagcgct cctgtcagcg atcactgcgc
     3661 caatgtgtgt ggtatctaac ggcccaaata acaaaatgca gcattctatg ggcaagctga
     3721 atatgttgca ctacttcccg gataaactgt tcagcggcta cgatattcag cgctggaagc
     3781 cagacccggc gttaatgttc catgcggcaa aagcgatgaa tgtaaatgta gaaaactgca
     3841 ttctggttga tgactcagtt gccggtgcgc aatctggtat cgacgcaggt atggaagtgt
     3901 tctacttctg cgccgacccg cacaataagc cgatcgttca cccgaaagtc accaccttta
     3961 cccatctttc gcagttaccg gaactgtgga aagcgcgtgg ttgggatatt acggcatagt
     4021 tcttcacact cccttcactt accccgctta aattggcgct caaaggtaag taaagggagt
     4081 ttgatatgtc tgtttcacgt cgggtaatac atcacggact ttattttgcg gttttaggac
     4141 cgttaattgg tgttctgttt cttgtcctct acatattctt cgcaaaagaa ccgctggttc
     4201 ttttggtaat aatacaggta cttccattgt ttctcttatt gtcgattact acgggagcta
     4261 ttcctgcatt gttaaccggc gtgatggtcg cctgtctgcc ggaaaagatc gggtcacaga
     4321 aaaattatcg ttgtctggct ggtggcatag gtggcgtcgt tatcaccgag atctattgtg
     4381 cagttattgt acatattaag ggcatggctt cctcggagtt gtttgaaaac attctttctg
     4441 gtgacagtct cgttgtccgc atcattcctg cattgctggc aggtgtggtg atgagcagaa
     4501 tcattacccg tctgcccgga ttggatattt catgtcctga aacagactct ttaagttaag
     4561 cgggatactt tatctttggg ctactcaaaa gcagacagga tgttactatg actcaaaata
     4621 tcaggccgtt accccaat
//
LOCUS       DAETQL010000191         4635 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.195, whole genome shotgun sequence.
ACCESSION   DAETQL010000191 DAETQL010000000
VERSION     DAETQL010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.195"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..374
                     /gene="bglB"
                     /locus_tag="K0W65_004126"
     CDS             <1..374
                     /gene="bglB"
                     /locus_tag="K0W65_004126"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HBI3097152.1"
                     /translation="LRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYL
                     NDHLVQVNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRT
                     RKKSFGWYAEVIKTRGLSLKK"
     gene            460..2076
                     /gene="bglH"
                     /locus_tag="K0W65_004127"
     CDS             460..2076
                     /gene="bglH"
                     /locus_tag="K0W65_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HBI3097153.1"
                     /translation="MFRQNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            2103..3278
                     /locus_tag="K0W65_004128"
     CDS             2103..3278
                     /locus_tag="K0W65_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBI3097154.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            3280..4002
                     /locus_tag="K0W65_004129"
     CDS             3280..4002
                     /locus_tag="K0W65_004129"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBI3097155.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(4064..>4635)
                     /locus_tag="K0W65_004130"
     CDS             complement(4064..>4635)
                     /locus_tag="K0W65_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HBI3097156.1"
                     /translation="GALTFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVD
                     EVEHLCPLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELV
                     ERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLA
                     KCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
BASE COUNT         1285 a         1026 c         1132 g         1192 t
ORIGIN      
        1 gattacgggt tctgttaaat acgctttggg atcgttatca aaaaccgtta tttattgtcg
       61 agaatggatt aggcgtaaaa gacagcgttg aagcggatgg ttcgatacag gacgattatc
      121 gaattgccta tttaaacgat cacctggtac aggtaaatga agcgattgcc gatggtgtgg
      181 atattatggg gtacaccagt tgggggccaa ttgatttagt cagtgcatct cattcacaaa
      241 tgtctaagcg ctacggcttt atttatgtgg atcgtgatga taatggcgaa ggaagcctca
      301 caagaacgcg taagaaaagc ttcggatggt atgcagaagt gatcaaaacg cgggggctgt
      361 cattaaaaaa ataaccaata aagcacctta attatcgtcg cattcagaac agtctggatg
      421 cgatacgtta attctttctt tgcaccataa agggatatta tgtttagaca aaatcttatt
      481 acctctgcca tcttattaat ggcaccgtta gccttttctg cacaatcatt ggctgaatca
      541 ttaacggtgg aacaacgcct tgagttatta gaaaaagcgt taagagaaac gcaaagcgaa
      601 ctcaaaaagt ataaagatga agagaagaaa aaatatacgc cagcgacggt gaatcgtagc
      661 gtaagtacga atgatcaagg gtatgccgcc aatccgttcc cgaccagtag tgccgcaaaa
      721 cctgatgctg tactggtcaa aaatgaagag aaaaatgcca gtgagacagg ctcgatttat
      781 tcttccatga ctctgaaaga tttcagtaag tttgtgaaag atgaaattgg ctttagttac
      841 aacggctatt accgttctgg ttgggggacc gcctctcatg gttcacctaa atcatgggcg
      901 attggttctc tgggccgctt tggtaacgaa tactccggct ggtttgattt gcagttaaaa
      961 caacgtgtct acaacgaaaa cggcaaacgg gttgatgccg ttgtgatgat agatggtaac
     1021 gttggtcagc agtactctac cggctggttt ggcgataatg ccggtggcga gaactttatg
     1081 cagttctccg atatgtacgt taccaccaaa ggtttcctgc cctttgcgcc agaggctgat
     1141 ttctgggtgg gtaaacacgg tgcgccgaaa attgaaatcc agatgctgga ctggaaaacg
     1201 cagcgtactg atgctgcagc gggtgtaggt ctggaaaact ggaaagtcgg tccgggtaaa
     1261 attgatatcg cgctggttcg cgaagatgtc gatgattacg atcgcagcct gcaaaacaaa
     1321 cagcagatta atacccatac tattgattta cgctataaag atatcccgtt atgggataaa
     1381 gcgaccttaa tggtgagtgg tcgttatgtc acggcaaacg aaagcgcatc ggaaaaagat
     1441 aatcaggata ataacgggta ttatgactgg aaagatacct ggatgttcgg cacatcttta
     1501 acgcagaaat ttgataaagg tggcttcaac gaattctcct tcctggttgc gaataactct
     1561 atcgccagga actttggccg ttatgctggc gcaagtccat ttaccacctt taatggtcgt
     1621 tattatggtg atcacaccgg cggaacagca gttcgtctga cctcgcaggg cgaagcctat
     1681 atcggcgatc attttattgt ggctaacgcg attgtttact ccttcggtaa caatatttat
     1741 agctacgaaa caggcgcaca ctctgatttc gaatctattc gtgcggttgt tcgcccggcc
     1801 tatatttggg accaatataa ccagacaggt gttgaactgg gctatttcac ccagcaaaac
     1861 aaagatgcga atagtaataa atttaatgag tctggttata aaaccacgct cttccatacc
     1921 tttaaagtca ataccagtat gttgacctcg cgtctggaaa ttcgtttcta cgccacgtat
     1981 atcaaagccc tggaaaacga actggatggc ttcaccttcg aagacaataa agacgcccag
     2041 tttgctgtcg gtgcccaggc tgaaatctgg tggtaatgga acttaatgaa aggaatatta
     2101 cgatgaatat aaaaattgct gctttaacgc tggctattgc cagcggtatt tctgctcagt
     2161 gggccatagc agcggatatg ccagccagcc cggcacccac tatcccggtg aaacagtatg
     2221 tgactcaggt caatgccgat aacagcgtga cctttcgcta ctttgcccct ggggcaaaaa
     2281 atgtctctgt agtggtgggt gttccggttc cggacaatat tcacccgatg accaaagacg
     2341 aagcaggagt ctggtcgtgg cgcacaccta tcctgaaagg caacctgtac gagtattttt
     2401 tcaatgttga tggtgtacgc agcattgata caggcaccgc aatgactaag cctcagcgcc
     2461 aggttaactc cagtatgatt ctggtgccag gcagttatct ggatacgcgt tctgttgcgc
     2521 atggtgattt gatcgccata acttaccact ccaacgcatt gcaatctgaa cgtcagatgt
     2581 atgtctggac cccgccagga tacaccggca tgggcgagcc tttgccagtg ctctatttct
     2641 atcacggttt tggtgatacc ggacgttccg ctatcgatca ggggcgtatc ccgcaaatca
     2701 tggataacct gctggctgaa gggaaaatta aaccgatgct ggtggtgatc ccggataccg
     2761 aaaccgatgc gaagggcatt attcccgaag atttcgtgcc tcaggaaaga cgtaaagtct
     2821 tttatccgct gaatgctaaa gcggcagatc gcgaactgat gaacgatatt atcccgctga
     2881 ttagcaagcg ttttagtgtc cgtaaagatg ccgatggtcg cgcgctggca gggctttcac
     2941 aaggcgggta ccaggcgctg gtttccggaa tgaatcatct ggaaagcttt ggctggctgg
     3001 ccacattcag tggtgttacc acgacaaccg taccggatga aggtgtcgcg gcccggctga
     3061 acgatccggc agctattaac cagcaactac gtaattttac tgtggtcgtt ggtgataaag
     3121 atgtcgtaac cggcaaggat atcgccgggc tgaaaactga gcttgagcag aaaaaaatta
     3181 agtttgatta ccaggaatat cccggcctga accatgaaat ggatgtctgg cggcctgcct
     3241 atgcagcctt cgtacagaaa tttaaaataa ggcattaaga tgaaattaat cattaccgaa
     3301 gattaccagg aaatgagccg tgtcgcggca caccatctgc ttggttatat gtcgaagacg
     3361 cgtcgtgtta acctggcaat taccgccggt agcacgccca aaggcatgta tgaatacctc
     3421 accaccctgg ttaaaggtaa gccttggtac gataactgct atttctataa tttcgatgaa
     3481 attccctttc gcggcaaaga gggagaaggc gtaacgatta ccaatctgcg taatctgttt
     3541 ttcacccctg cggggatcaa agaagagaat atccagaagc tcactattga taactaccgc
     3601 gagcatgatc agaaactggc gcgtgaaggc ggcctggatt tggtggtgct gggattaggt
     3661 gcagatggtc atttttgtgg caatttgccg aatacgaccc atttccatga gcaaaccgtg
     3721 gagttcccga tccagggaga gatggttgat attgtggcgc atggtgagtt gggcggcgac
     3781 ttttcgttag tgcctgacag ctacgtgacg atggggccga aaagcatcat ggcggcgaaa
     3841 aacttgctga taattgtcag tggcgcaggt aaagcgcagg cactgaaaaa tgtgctgcaa
     3901 ggtccggtta ccgaagatgt tccagcttcc gtgttgcagc tgcatccttc gctgatggta
     3961 atcgctgata aagccgctgc cgcagaatta gcgttcggtt aagctgaaga aataaggccg
     4021 gatgtatgaa ttacgctccg gcctgttctc attatttaaa taactacgaa aaatcacccc
     4081 acagtctcag cttgccgcag tggagacagc ggaagagata accctgacaa tcaccaccct
     4141 tttgcaggca tttagctaga tcagaatttc taataccgaa attctcacaa tcttcttcaa
     4201 ggttggcaaa ttcatcgagg cgatctttta tatcattcca gcccacatag ccgataaaag
     4261 cacagaaatc gccacaatgc gcaagccaga attcctgttg ccatccatga taacctggcg
     4321 tgcgttcaac caactctttc agcatttcat caggatatgt gttcttaata ccggcaaatt
     4381 cgtcctcttc atcatactca aattcaacac cttctatgct ggcatcatcc tgaaaactac
     4441 ctgcaaactt ttctgcagca gaaccgtccg caatacacaa cggacagaga tgttcaactt
     4501 catcaacgca ataaaaggga cccgagtaat aaactgacgt ctgttgttca cagcaatcgc
     4561 actctacggt tttatcctgt tcaaatgcgc ctgtttccag tggcttggga tgatatttga
     4621 atgtaagcgc cccga
//
LOCUS       DAETQL010000192         4603 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.196, whole genome shotgun sequence.
ACCESSION   DAETQL010000192 DAETQL010000000
VERSION     DAETQL010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.196"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(79..867)
                     /gene="phoH"
                     /locus_tag="K0W65_004131"
     CDS             complement(79..867)
                     /gene="phoH"
                     /locus_tag="K0W65_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HBI3097157.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="K0W65_004132"
     CDS             complement(1474..2739)
                     /gene="efeB"
                     /locus_tag="K0W65_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HBI3097158.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="K0W65_004133"
     CDS             complement(2745..3872)
                     /gene="efeO"
                     /locus_tag="K0W65_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HBI3097159.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3930..>4603)
                     /locus_tag="K0W65_004134"
     CDS             complement(3930..>4603)
                     /locus_tag="K0W65_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HBI3097160.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1117 t
ORIGIN      
        1 tttcagaaaa agcgctagtc cgggcataaa cacccaaaac aaagcccggt tcgcccgggc
       61 tctgcaccga taatacactt aactgtatgc atgcagcgta cgttggcaaa gtgccgaacg
      121 tacgcaatcc tcttttccga accggacgat cccaaccatt tcatcttctt cgaaacgttc
      181 cagcgcgtca cttaatccgg agcacacgcc gcgaggcaaa tcgcattgcg tgatatcacc
      241 gttgacgata accgtcacgt tctccccgag gcgggttaaa aacattttca tttgcgcggc
      301 agtcacattc tgcgcctcgt caagaatgac aactgcattt tcaaaggtac gtccacgcat
      361 ataggcgaac ggcgcggttt ccaccttccc tatttccggt cgcaggcagt actgcataaa
      421 ggaagcccct aagcgccgga ccagcaagtc gtagaccggg cgaaaatagg gagcaaactt
      481 ttctgcgata tcaccaggta agaagccaag atcttcatcg gcttgcagaa ctggacgggt
      541 gacgataatc ctgtcgacat ccttatgtat cagggcctct gccgcttttg ctgcgctgat
      601 ccaggttttt ccgcacccgg cttcgcccgt ggcgaatatc agctgcttac tctcaatagc
      661 ctgcagatag tgcaattgcg cttcatttcg cgcaaggatg ggcgaggtat cgcgactgtc
      721 gcgggccata ccaatggctt ctacgccgct catctgcaca agcgaggtga ccgattcttc
      781 ttcacgctgc ttatggctgc gcgaatcccg tctcagcaca cgttttgcct cgcgacgagc
      841 tttgatcact gctttttgtc ttcccatgga gagcaccttg agttgtttgt attcatcaca
      901 cgcgccgttg gcagcgcgat tatgcgcacg aacatcagag ggttggcttc cttgtaagcc
      961 atagtttgct tttggataaa atgccgaaaa acggctacgc acaccgtttg cggcgtcggt
     1021 aacacgagta aagaaaggaa gtgagaaaaa tacagagtga cgaagttgct accggaggag
     1081 aaatctccgc gagtggtgga gcgttgctta acaaaacatt gtccagaaca ggacgttacc
     1141 atccgcgatc tccatagtga ctgactatca ctgccgggaa cttccgctgc tacttaataa
     1201 gtacaacaga actcgcattc attgcaacaa tatattcact tatatttaac gataacaccc
     1261 atttcggtga cattaatttc tactggaaag atgacagagt gatgacagtg atggaaaaag
     1321 ctgtgtgctt tccgcaggat tcgcagggct ggtaaaatgt gcctcgtgtt tgccggattc
     1381 ggcgcgaacg ccttacccga cctacaagtg attaataatt tgtgcacaaa actcatgccc
     1441 gataaacctg cgcatcgggc atatttgcgt cgttcaaacc tgcaatagcg cccttcccag
     1501 ataatcgttc gcgtccttca ctcccggcag cgaaaaaaaa taaccgccgc cgataggttt
     1561 aacgtattcc tccagcgctt cgccattgag ccttttttgt actgtcagga agcctttttc
     1621 cagatcgtgt tggtagcaga caaacagcaa ccccatatcc agttgtccgg agttggtgac
     1681 ccccaacgaa taactgtagc cgcgacgcag catcaggctg gactcactct ccgccgtgcg
     1741 gggattcgcc agccggatat ggctgtccag cgcgatcacc ttcccttccg ggtcgctggc
     1801 gtaatcaggc acatcatgct catgctgcat tcccagcggc gcaccggttt gcttatcacg
     1861 gccaaaaatc gtctgctgtt ctttcagcgg cgttctgtcc caaaattcca ctcgaaactg
     1921 aatcaagcgt actgcctgat agctgccacc gattgtccac gcaggctcct gctgatctgc
     1981 cgttacccac accacttttt gcatcaactt atcattctgg ctatcgggat tggcagtgcc
     2041 gtctttgaaa cccagcaaat taatcggcgt ctctttgcct ttactacgcg ccgcgtgatc
     2101 ggaaataaac ccttcccgct tccagcgcac actgagcaaa tccggcgtgt gtttgatgat
     2161 atcgcgcagc gcatggttca ccgtgtcctg ggtgttggcg caaatctgta gcaacacatc
     2221 accatgacat aacgccgcat ccagcgagtc gttggggaaa cgcgtcatct tctgcagctt
     2281 ttttggcatc tgtggcgcaa ggccaaagcg ctcatcaaac aatgagtgac ccaccgataa
     2341 cgtgatggtg agattatcgg gcgcaatgta gccgccaaga atgccggaat cgagtggtgg
     2401 caggcgcgga tttggcgttt ctggtgctgc tccgccctga ctcagaaaag caaaacgctg
     2461 agtcaacaag cgaaacaacc gctcaagatc ggctttatcg ctggcaagca catcaaacgc
     2521 caccagcatc attgcggcct gttgtggcgt caggatccct gcctgatgct caccataaaa
     2581 cggctgtttc tcattgcgtg catccggtga aagcgtgccc ggtgcacttt gcgttttttg
     2641 tgcatgagcg accggacaac ttcccgccag cgcaccaatc cctttcagta aacgtcggcg
     2701 tgacggttca ttcacgccgt tttcatcttt atactgcata acgcttaatc cagtcccagc
     2761 acaccgcgaa gttgcgccag atcttccgcc agcgcagtaa tcggcccttt cagcgcattc
     2821 cggtcggcat cggtcaattt gtcgtaggtt tcaaaaccgt ctttagtacg gtatttcgcc
     2881 agaatggtat cgaccttttt aaagttggca tcgacttttg ccaacagttc cgggttggct
     2941 ttttgcagtt gtggacgcag cagatcgaca attttctgcg agccttcaac gttagcctgg
     3001 aaatcccaca gatcggtgtg gctgtagcga tcttcttcac cgctgatttt gctggctgcc
     3061 acttcctcaa tcagtccggc tgcgccgccg accacttttg aaggtgggaa agccagttca
     3121 ctgatgcgtt tttgcaaatc gaccacatcg gtataaagct gctcagcgta ctgatccatc
     3181 cctttggtgg tgttgtcgcc aaacaatgct ttttccagac ggtggaaacc agtgaatttt
     3241 gggtcggcgg ctttttgctc gtaatcatct tcacgggcgt caatgctgcc atccagatcg
     3301 gagaacagtt cagcaatcgg ttcaatacgc tcatagtgct ggcgcgtcgg cgcatacagt
     3361 gctttcgctt tttcgatatc gcctgcttta atcgcgtcgg taaaggcttt ggtgtcggtc
     3421 accagctgcg tggtttccgc catgacatac gctttatatg cagtaattgc accaccaaga
     3481 cttaacagcg catcactttg cgccgcatcc gccgttgcct cacctttgac gatcaacttc
     3541 cctttcgggt tagtcagcag accgcaggtc atatcgtatt cgccaggctg taaattcgcc
     3601 gtcattttct ggctaaaacc aggggcgata ttttcccgct cttccaccac catcacgcct
     3661 ttgaggatct cccactccag cgccttctgg ctatggttct gaataatgaa ctgtgttttc
     3721 ccggcgttaa ccgtaatggt catcggttcg cactgcttat ccgtcacggt cactttgacc
     3781 tgcggcacat cagcggcgtt agccataaaa gcagaagaaa acagcgcagc cacgctcaac
     3841 tgcaatgcgt tacggcggaa gttaatggtc atgacgagac tatcccttta aagtatgttg
     3901 taactaagca agagtttgcg tcgtatttgt tacgcggagc gagacgctgt cgcccctgcg
     3961 cgtggtggca gagcaaatgc caccagcgcc gggatgagat aaataaacca gacggcgact
     4021 tcgctgacgc tcggtgcttc ctgatagcca aaaatccctt ccatcagcgt gccaaacagc
     4081 gagtgagttg agaggaccgc gctcatatcg aaggcgattt cctgaaagtg gttccacaat
     4141 ccggcttcat gaaatgcgcg aatggcacca gccgccagcc ctgcggcgac gaagagaata
     4201 aacaggctgg tccatttaaa aaatgcacca agattgaggc gaataccgcc ccagtagagc
     4261 aggaagccga gcaccacggc agtagcaaga ccgagcattg cacccagcgg cggccagatc
     4321 ccgacatctt gttgaaatgc cgccagcagg aaaaagaccg actccagccc ttcccttgca
     4381 acggcaaaaa agaccatcat caccagcgcc cagccatgat gatttccacg ctgcaatgcg
     4441 ctatcgactg cctgttccag ttgcactttg acgttgcgcg acactttgcg catccagaaa
     4501 accatccagg taaggatcac cacggcgatc accgccacga taccttcaaa cagttcctgc
     4561 tctttttgcg gaaattcgcc ggtggtttcg ttaatgaaga tcc
//
LOCUS       DAETQL010000193         4550 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.197, whole genome shotgun sequence.
ACCESSION   DAETQL010000193 DAETQL010000000
VERSION     DAETQL010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.197"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..1224
                     /locus_tag="K0W65_004135"
     CDS             <1..1224
                     /locus_tag="K0W65_004135"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBI3097161.1"
                     /translation="FPYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLY
                     PLSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGIS
                     NYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGV
                     TNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEK
                     WFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILP
                     VIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHL
                     LIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRA
                     ARVFVNDERFTRGGN"
     gene            1224..1958
                     /locus_tag="K0W65_004136"
     CDS             1224..1958
                     /locus_tag="K0W65_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3097162.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2426..3187
                     /locus_tag="K0W65_004137"
     CDS             2426..3187
                     /locus_tag="K0W65_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBI3097163.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3231..4310
                     /locus_tag="K0W65_004138"
     CDS             3231..4310
                     /locus_tag="K0W65_004138"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI3097164.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4307..>4550
                     /locus_tag="K0W65_004139"
     CDS             4307..>4550
                     /locus_tag="K0W65_004139"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBI3097165.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1247 a          864 c         1120 g         1319 t
ORIGIN      
        1 tttccgtatg gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc
       61 ggcgtagcgt tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg
      121 ttgtcgttat accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt
      181 ggtttatttt gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt
      241 ccccttagcg gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt
      301 atctcaaact atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat
      361 gaccagcgta tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc
      421 ggttgggggt taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg
      481 acaaacatag agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct
      541 gaaggtggca tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg
      601 tgtatatgcc gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt
      661 attgccttaa tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg
      721 tctgttcccc atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg
      781 atgaatcagg tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc
      841 attatgaccg ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc
      901 agagctgaag ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa
      961 tacctgacgc aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg
     1021 gggttcgttc gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg
     1081 gcaatccaca atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt
     1141 gattatctga cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa
     1201 cgttttactc gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca
     1261 caaacatcaa ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga
     1321 ttggcctgga tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag
     1381 tgaaacttca ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca
     1441 tcgtgatatg ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga
     1501 aggtttgtcg tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag
     1561 gaatgacgaa tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag
     1621 tggatatttt attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt
     1681 tgtttataaa acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga
     1741 ttagcacacg taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg
     1801 agtccgcaat tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac
     1861 gtaatgctca acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggtatg
     1921 aaagatgtaa caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct
     1981 ctttttcgat gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat
     2041 gttttgccac gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa
     2101 agagagttaa taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt
     2161 tgttttttaa attttcaggt tttggttaaa tatttgcttg caaatatagg tgattttttt
     2221 attgtaggct tttgaaggct attataactg tgcgctaaat taatgcgttg aataaaagaa
     2281 ataacaaaat taagaaaagc catatttacg atatgggcaa cataaatata atctaaaaaa
     2341 gagtaaggcg ttgtaggttg gttccgcgtt cattaccttg ttagtgaacg cggatttatc
     2401 agttgtcgaa atggttttta taataatgaa aaattttaat gtactaacca aagtcgtatt
     2461 ttgcttgtcg tttttatcaa gcgcatttcc cacctatgct gcggatgatg tcaaagtgac
     2521 tcaaaacact tcagttttta aactgaagct tagtgcaacg cgattaatct atgaacaagg
     2581 gtcggatggc gcagcgataa ctgtgacaaa tccgcaggat tggccgatgc ttgtgcagag
     2641 taaagtcttt gaagaagata aagtaactgc agcgcccttt attgtcactc cgccactgtt
     2701 caggcttaat ccaggccagc aaaataaaat gtgggttgtg cagactgcgc cagctttaca
     2761 agacagggaa aaattacact ggctatgcgc caccggcatt acgcctaaaa agagtgatgt
     2821 ttgggccgag aaagataaag aaaaaattgc tgaaaatgtg aacctgaaca tccaggttaa
     2881 cgtacatacc tgcataaaac ttattaccag gccatctggt attgaaggca agatggttaa
     2941 tgcgagtgag aagttatcgt ggaaaaagca gggtgataaa attgtcgcgg tcaatcagtc
     3001 gccgttctac atcaaccttt cttctttaca ggttggcggt agcaagattg agaaactaga
     3061 ttatattgcg ccgttttcgc aacatgagtt tgttatgcca aaaactagcg gccaggatat
     3121 taaatggtct gtgattactg atgaaggtgg tgagagtcgt ttgtttatat ctcatttgca
     3181 atcataatgt attacatttt tattgaatat ttttatgcca tgcatattcc atgttttatc
     3241 gactgtcatt aattttgata atggccttac ttgcgggtca gttaagtgcc caggagtggt
     3301 catttgattc ttcacagttg gaaggaaacg ttagtgcgga cactgtggca atgtttaatc
     3361 agggagagca actccccgga aattatcgtg tggaaattta tcttaacggt gaaaaagttg
     3421 atgttgggga gtttcccttt catcgtccgg aatcccctga agaaaaagag cttgttccat
     3481 gtttaaccgt tgatgacctt atccactatg gcattaaaat tgataaaagc agcagtgata
     3541 ctgacaataa aaagaatcag tgttttaaat ggaactctat tgaaggtttg aaagtaaatt
     3601 atgattttga tagccagcgc gtacagataa ccgttccaca actgtatctg caagataaaa
     3661 aaagtagttt agcaccagtg tcgttatgga atgagggtgt agctgcattc cgcatggtgt
     3721 atcagacaaa tattgatatt tccaaacaaa atgataatca atctaccacg cgtaattctc
     3781 gttatggcag gttcactcca gggtttaatc ttggtgcctg gaggtttcgt agttcagtga
     3841 catggagcaa ggaacttggc cagtcagaaa gatggcaaag gggttacatg tggtttgagc
     3901 gaggcataaa tgcgattaaa agccgattaa cgcttggaga atcttatacg tcgtctgagg
     3961 tgtttgacag catacctttt cgcggcggga tgttagcgac cgacgatgcg atgactccgc
     4021 cggaggacag ctactacaca cctgtggtgc atggaatagc acaaagtgag gcgcaggtta
     4081 ttattaaaca aaatgggcaa attattttta cccgttctgt gccgccaggg ccttttgcgc
     4141 tggataattt accaacgctg gccgttggcg gggagcttga tgttacagtg agagagagta
     4201 atggcgagga gcagtatttt tccgtgccat ttcaaacgcc ggccatcgct ctgcatgaag
     4261 gatatttcaa atatagtgtg atggggggga atataaagaa gaaggtataa aaagctcccc
     4321 tgtctccgtg ggcgaaatca cggcgatgta cgggcttcct tatggcgtca cggtgtatgg
     4381 agggattcaa aacgccactc attttaatgc aatatctacg ggtattggca tttctctggg
     4441 gctgttaggg agtttatcga cagatattac gcgttcgatt gcgaatttat attatgggaa
     4501 taaatatcgt attcgttata gtaaatccat tagtgatttt ggtactcaat
//
LOCUS       DAETQL010000194         4422 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.198, whole genome shotgun sequence.
ACCESSION   DAETQL010000194 DAETQL010000000
VERSION     DAETQL010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.198"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..171)
                     /locus_tag="K0W65_004140"
     CDS             complement(<1..171)
                     /locus_tag="K0W65_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3097166.1"
                     /translation="MSRKTQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGS"
     gene            complement(235..426)
                     /locus_tag="K0W65_004141"
                     /pseudo
     CDS             complement(235..426)
                     /locus_tag="K0W65_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215564.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(567..2600)
                     /gene="metG"
                     /locus_tag="K0W65_004142"
     CDS             complement(567..2600)
                     /gene="metG"
                     /locus_tag="K0W65_004142"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBI3097167.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2732..3841
                     /gene="apbC"
                     /locus_tag="K0W65_004143"
     CDS             2732..3841
                     /gene="apbC"
                     /locus_tag="K0W65_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBI3097168.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4103..4384
                     /locus_tag="K0W65_004144"
     CDS             4103..4384
                     /locus_tag="K0W65_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HBI3097169.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1071 a         1102 c         1118 g         1131 t
ORIGIN      
        1 ggaaccagga gtaccgagcc cttttctggc ggcggtaacc cattgtccta aagtgccttc
       61 aggaagagat aatcgggagg cgccttcact gatcgaaagt tgattttcaa gaaccgttct
      121 gacagcttcg gctttgaact ctttagagta acgttgggtt tttctgctca ttattagctc
      181 cttctgatgc cattctattt caggaaggag tgtccgttaa actcaggcta cctcataccc
      241 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
      301 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      361 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      421 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      481 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      541 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      601 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      661 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      721 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      781 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      841 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      901 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      961 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
     1021 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
     1081 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagc
     1141 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
     1201 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1261 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1321 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1381 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1441 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1501 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1561 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1621 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1681 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1741 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1801 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1861 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1921 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1981 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     2041 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     2101 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     2161 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     2221 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2281 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2341 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2401 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2461 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2521 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2581 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2641 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2701 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2761 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2821 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2881 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2941 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     3001 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     3061 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     3121 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     3181 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3241 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3301 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3361 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3421 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3481 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3541 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3601 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3661 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3721 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3781 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3841 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3901 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3961 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     4021 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     4081 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     4141 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     4201 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4261 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4321 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4381 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAETQL010000195         4401 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.199, whole genome shotgun sequence.
ACCESSION   DAETQL010000195 DAETQL010000000
VERSION     DAETQL010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.199"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            89..727
                     /locus_tag="K0W65_004145"
     CDS             89..727
                     /locus_tag="K0W65_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097170.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKW"
     gene            complement(999..1694)
                     /locus_tag="K0W65_004146"
     CDS             complement(999..1694)
                     /locus_tag="K0W65_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HBI3097171.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1687..3114)
                     /locus_tag="K0W65_004147"
     CDS             complement(1687..3114)
                     /locus_tag="K0W65_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HBI3097172.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3125..3844)
                     /locus_tag="K0W65_004148"
     CDS             complement(3125..3844)
                     /locus_tag="K0W65_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HBI3097173.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            4263..>4401
                     /locus_tag="K0W65_004149"
     CDS             4263..>4401
                     /locus_tag="K0W65_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBI3097174.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YT"
BASE COUNT         1162 a         1135 c          915 g         1189 t
ORIGIN      
        1 gttttttgct gatatttaac ccgaacattc ctgatacaga ctattaatac aacattatct
       61 gcgacagatt ttattttctg gatatattat gagtgaacaa atcaaacagg atatcgatct
      121 gattgagatt ttatttcatc tgaagaaaaa gattcgtgtt atccttttta ttatggctat
      181 ctgtatggca atggtgctgt tgtttctgta tatcaataaa gacaatacaa aagtgattta
      241 cagccttaaa ataaaccaga caacgccagg tatacttgtt agctgtgata gcaataataa
      301 ttttgcctgt cagaccacaa tgactgaaga tgttattcag cgaattacta cattttttca
      361 gaccagccca gatatcaaga acagagaaat aaggctggaa tggtcaggag ataagagaga
      421 tttaccaact gctgaagagg aaatatctcg cgtgcaggcg tctattatca aatggtatgc
      481 gtcagaatac cataatggca ggcaagttct cgatgagata caaacgcctt cagcaattaa
      541 cagtgagctt tatacaaaaa tgatatacct gaccaggaac tggtcattgt atccgaacgg
      601 tgatggctgt gtaactataa gctcaccaga aataaaaaat aaataccctg ctgccatttg
      661 cctggctctg ggattttttc taagcattgt aatttctgta atgttttgcc ttgtcaaaaa
      721 atggtagatg aataccaaca aaactctggg cagtaataaa cagcattctg atagagagtg
      781 cctgtataaa gctgaagtga caatgaccgg gaagggaacc cgttttcagg aatggatgcc
      841 tgtctttttc actgtaaata tcctggcatt actaatgcct ggatatttac agaagagcag
      901 cataaccatg acggaacggg gttaagtcag gttgtcctta acgctatttt caacctcgta
      961 tgccttcttc aggtttatgt tcagatctca tatctctctc agcaatcctc aataatcaga
     1021 tacaccgctt tcaccggacc atgaaccccg acgactttga taagctcaat atccgccgtt
     1081 gagctgggac cactgatgat gttaatacat gacggcattc gttcaccggc ctgcgctttc
     1141 tgatgcaatt tttctgcgag ttgcgctaca cgcggcagga ttgtgctttt acgcaggata
     1201 aaaagagaag attccgggag caggctcaat gaacgcccgc gctcggcggc ggaaaaaaga
     1261 accacgcctc ccgattcggt taaaccatat tcagcataca cgacacccac tttagcctgc
     1321 tctgcctgcg agatattctc ggcacctttc gccggatccc aaacaacggc attgcattcc
     1381 tgctgcaaac gttcactaat ccccaattcc tccagcctcg tgtcaccgct aatcacgacc
     1441 gactgatctc ccagctcttt acacagacgt attgcagctt ctgccgcctt cgcctcgctg
     1501 gtcagctcac agcgcgtcaa cataacatcg ctggcaaact gaataaacgc gtcacagcgc
     1561 tgctgttggt taagttgggt aagccgctcg ttagcatagt tgttaagcgg cgcatcttct
     1621 gcttgcggtt caagtcgcag cgggcgaccc agtgcctgag caacgttatt caaaaattcg
     1681 ctccgattat ccattctttt tctcctgcgc ctgatgtttc ttaaaccaac tacggaaact
     1741 ctctccgtca gcttcaggaa gatcgcgtgc ttccatccag tcgctaatcg cgccaaattt
     1801 gagtggtgtt ttgccgccat tgataaacca gcttgccgca tgagcgccgg ccatcatccc
     1861 gactttccac aatcctggat gactattggc ataagcgaac atttttatcg cccgttgctc
     1921 tgcttttgcg gtgatccctt tttcagccat cacccgacga tgacgcaaaa tcagttttga
     1981 cagcggaata cgcaccggac acacgctgtc acaagcagtg cataaagagc aggcgtaggg
     2041 taaatcttta aaatctttat agccgccaag tagcggagaa atcaccgcac caattggccc
     2101 tggataaata gagccatatc catgaccgcc aatatggcga tatgccggac aagtattcat
     2161 acaagccccg cagcgaatac agcgcagcac atcccgaaat tcagaggcca gcacctcaga
     2221 acgcccgtta tcgacaataa ccagatgaaa ctcttcagga ccatcaacgt gccccgcttc
     2281 gcgcggccct gtcagccagg tgttgtatcc cgtcaaacgt gcaccaacgg cactgcgcgc
     2341 cagcatggtg atcaatacat ctacctcggc aaacgtgggg gcaatacgct ccattcccat
     2401 cactgcaata tgcgttttag gcagcgtggt acacattcgc gcattacctt cattggtcac
     2461 caggcatacc gaaccggtct ctgccaccgc gaaattacag ccggtaatac ctatttcagc
     2521 actgaggaaa tcttcacgga ttttttgccg gatgaataag gtcatcgctt caggcgtttc
     2581 cggcccctca tagcccagac gttcgtgtag cactcgacgg atctgatggc gatctttatg
     2641 aattgccggg accacaacat gagatggcgg atcttgatcc agctggagaa tatattcacc
     2701 cagatcggtt tcaatcacct gaatgccagc atcctgcaac acatgattga caccaatctc
     2761 ttcggtcacc atcgatttag atttcaccac cttccgggca tttttgcgtt gggcaacctg
     2821 taaaatgtag cgggtagcgt cttctttggt ttttgcaaaa tagacgtgac cgccgttttg
     2881 cgtcactttt tctgagagct ggtacagata agcgtcgaga ttactcagaa catgatcacg
     2941 tatctgggcg gcccgatcgc gccactcctc ccagtgcccc aattcatcga ccattttttg
     3001 ccgatttgcc ccaatacgct gctgcgcgct tgccaccgct ttgcgcatga tcggatcttc
     3061 aatttgctga cggatgcgtg tcttaaaatc tgtattactg gttttgatcg acatctttat
     3121 atcctcagcg gctcatcaac acttcagcaa tatgcatcac tttgactttc tgcccttccc
     3181 gttgtaatcg cccactgatg ttcagcaggc aactcacgtc agcaccaatt aaatactcag
     3241 ggcggacttc catcaggtgc acaacctttt ctttcaccat ctcgccggat atttcggcca
     3301 ttttgaccga gaacgtgccg ccaaatccgc agcaggtatc ctgttcagca aaggtcaaca
     3361 gctccagtcc acgcacattt ttcagcagcg taagtggctc gtccttcact cccagcttac
     3421 gggccaggct acaagatggg tgatacacag ctctcccttg caaactggca cctacatcga
     3481 ctacccctaa tttattaaca ataaaagagg tgagatcctg catacgcgcg gcaacctttt
     3541 cggcacgtga tgcccattca ggttcatccg ccagatacgt cgggtaactt tttatggcat
     3601 aggtgcaaga gccagccggt gaaataatgg gatcgtcgtt atcctccagt gcggcgatca
     3661 gatttttcat ccctggaatc gcttctttga tataaccgct attgatcgca ggctgaccgc
     3721 agcatccctg tttctccggg aaatttacgc gacagccgag tttttccagt agcagcacgg
     3781 agtctcgtgc cattcttgat ttcagggcgt caccaataca ggtgacaaag aaattgacat
     3841 tcacaactaa tactccatta cttcatgccc atttatgccc gatgtttccc ccccaccggg
     3901 ggatcaatca tcaataaata tggcgttctt ttttaattgg ctagcaacat accacccctc
     3961 atttgtatga caaatgataa tctttcatcc aaacttcaaa tgcaataact aattttattt
     4021 ctttcggcat cgctcacatt ttatggggaa atcgacgatc atttttggca taagaaatcc
     4081 atttaaaaat ggctacatcc cttataccta ttcagagcta cttattcagg ggttattgag
     4141 atatacactg atggtgtaca aaaaaatcac cgacaataat cacgtatcaa gtaatagtcg
     4201 gaattttggt aatgctgcca acttactgat ttagtgtatg atggtgattt taaggtgctt
     4261 gcgtggcttc catttccatc agatgtcctt cctgctccgc tactgaaggc gtggtgcgta
     4321 acggcaaaag cactgccgga catcagcgct atctctgctc tcattgccgt aaaacatggc
     4381 aactacagtt cacttacacc g
//
LOCUS       DAETQL010000196         4373 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.200, whole genome shotgun sequence.
ACCESSION   DAETQL010000196 DAETQL010000000
VERSION     DAETQL010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.200"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            158..599
                     /gene="tnpA"
                     /locus_tag="K0W65_004150"
                     /pseudo
     CDS             158..599
                     /gene="tnpA"
                     /locus_tag="K0W65_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317271.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(717..1661)
                     /gene="hypT"
                     /locus_tag="K0W65_004151"
                     /gene_synonym="qseD"
     CDS             complement(717..1661)
                     /gene="hypT"
                     /locus_tag="K0W65_004151"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HBI3097175.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            2006..2725
                     /locus_tag="K0W65_004152"
     CDS             2006..2725
                     /locus_tag="K0W65_004152"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBI3097176.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2791..4089
                     /locus_tag="K0W65_004153"
     CDS             2791..4089
                     /locus_tag="K0W65_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HBI3097177.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT         1096 a          953 c         1074 g         1250 t
ORIGIN      
        1 gaacaaattg tcgcccattg tgagtcatat tcgctctgac tttccagaac catacggact
       61 gcccgttgac ggacttcggg ggaaaaacga gtatttttag tcatcctgtt tacctctttc
      121 tcaggaagtt tagtctccag gattcccggg gcggttcata gcgcacaccc gatggaactg
      181 taaatatcac atagtttttt gcgccaaaat accgaagaca ggtgttctac agagagaagc
      241 gtagagcaac aggcagtatt ttgagaaagc tgtgtgagtg gaaaagcgta cggattctgg
      301 aagctgaatg ctgtgcagat catatccata tgcttgtgga gatcccgccc aaaatgagcg
      361 tatccggctt tatgggatat ctgaaaggga aaagcagtct gatgctttac gagcagtttg
      421 gtgatttgaa attcaaatac aggaacaggg agttctggtg caggggggac tacgtcgata
      481 cagtgggtaa gaacacggcg aagatacagg attacataaa gcaccagctt gaagaggata
      541 aaatgggaga gcagttatcg attccctgtc cgggcagccc gtttacggac cgtaagtaac
      601 gaagttggat gcaaatgtca gatcgtgtgc gcctgttagg gcgcggctgg taagagagcc
      661 ttataggcgc atttgaaaaa cctccggcta tgccggagga tatttattgc acgtcgtcaa
      721 ttgcggcgtg ctggctcttc cattgatatc tgctggatta aatcgtctgg catatgttcc
      781 tgtaaaatac gccagaggtt ctcggcggtt ctattcagtc gggtgttcat tcggtaaata
      841 taagcctgaa tggggactgt caatttagca gcatcaaggc atatcagacg tttggtctgt
      901 agttcatcga cgatcgacca gatcggtaac caggcaatgc cgtatccgtt gagcgccata
      961 ttctttaata actcgctcat cgaagagata aatatcgtgc gcccggtaat acctccaacc
     1021 tctgccagat gacgattgac caacctgccc atataagatg tggcggtata gttgagcaaa
     1081 ggaacctggg gctgagtgat atcgaataca gggtgcccct gtgcgtcggc ggcacatacc
     1141 gggtacaact cagattcaaa cagtttcagg cagcaaaaag gcgattgcat aagatcttca
     1201 tcgcgaaacg aaataataaa gtcactcttg ccttcgcgta acgtatttac ggcctggaca
     1261 acatcaatgg cttcaacatg atagacaaat tctccgccat aggcggtaag tgaatgcacc
     1321 agtttgggta atacgctcag ggatagtgag tgcgcggctg caattttaat gttgggaacg
     1381 ccgagtaaac tctggccgtt taactccccc aaattgcatt caagctgctg taataagctg
     1441 cgagtctggg aatgaaagag cttgccctct tcggttaatt gtaaaggggt ggtcgtgcga
     1501 tcgaataaaa caacgccgat cgctgcctcc agcgccttaa ttcgtcggct gaatgcgggt
     1561 tgcgaaagat tacgctcttt tgccgcctga gaaaaatggc gacaggcttc cagtgtcagg
     1621 aaatcgtata accattttgt ctcgatattc ttcattacca tggcacctgt attttcaatc
     1681 caaagacata ataaaacatc ccggcaataa aaataatgtt ctcaattagc acaacctggc
     1741 ctttatcatt cgttttgtgc ataacccctt catttccggt attgacgttg atatcacatt
     1801 tcgccaaaaa aacgaaattt ttccgtcaga taaccagaat acatcatcac tatggtgaat
     1861 aagcggaatg ttccatagct gcatcatgcg tgtcttgcat cgccttgtac aaccttgcat
     1921 ttctcatcgc ccatttcgac tgttaaaaaa gagagcagca gttatttacc gcgtattgtc
     1981 attaacgttt tgagaggcag gtattgtgaa aggtttaatt ggtgtactcg gtggaatggg
     2041 gccggcagct accgtcgacc tgtttaataa atttgtaact tttaccgctg cacaatgtga
     2101 tcaggaacat attcctttaa ttatttcttc gatcccggat attcctgacc gtaccgatgc
     2161 attaatgcat catgggcgtt caccattacc cgttatgcgt gattatatgc ataagctgga
     2221 agatgccggg gcagaatgta ttgttattcc ctgtaatacc gcgcattact ggttcaaaga
     2281 attaaaagat gcttgtcata ttgatatatt aagtatcgta gaaactacga tcaatgaagt
     2341 tcgcgcctgt ggtaaaaccc gaattggctt gttagccacc aatgccacgc tatatatggg
     2401 tttataccaa aaaggaattg agtcgctggg atttacctgt gtcagccccg atgccgacgg
     2461 tcagaaaaaa gtgatggaaa gtatttattc cttgaaagct ggcaatatag cgcacgcaca
     2521 aaagttgatg aatgagcagg ctgaaatatt attttcccgc ggtgcagaaa tccttgttct
     2581 tggttgtaca gaaattccgg ttattctggc gcaagccgtt aaagagcagc cttcccgcta
     2641 tattgactca acggcgtcac tcgttcgtgc cggaataaaa tggtacgaaa atcgtgtcgg
     2701 taaacattat cttttaaccc aataatcatt taaatcgcag ccagaaagtt attcgctttt
     2761 aactgaatta tatttataac ggagaacatt atggtttggc tggaaattat cgtagtactt
     2821 ggtgcaatat tttttggtat tcgccagggg ggaatcggta ttggtttatg tggcgggctt
     2881 gggcttgcca ttctgactct gggatttggt ctgcctatgg ggtcgccgcc agtcgacgtg
     2941 atcctgatta ttatgaccgt tgttgtggcg gcctcagcct tacaggccgc aggcggaatg
     3001 gattatctgg tacgcgtcgc tggtaatttt atgcggcgta acccgaaata tatcaacata
     3061 atcgcgccga ttatcacctg gtctatgacc attatggccg gtactgggtt tattgttttt
     3121 tccacgctgc cggtgattgc tgaagtggcg aaagagtccg gtatccgtcc ttcccgcacg
     3181 cttgccggtt cggttgtcgc ttcgcaggtt gcgatttccg gttcgccgat cagcgccgca
     3241 atggcggcta tgctcactat tatggaggta aacggtgtca gttttattca agtgatgtcg
     3301 gtatgtcttc ccacctcttt tgctgccgcg atggtcgccg cttttattgc ttcccgacag
     3361 ggatgtgagt tgcaggatga cgaggtctac cttgagcgcc tgcaaaaagg actggtgcaa
     3421 aaatacgaga ataatagcag catcaagcca ggtgcgacgc tctccgtcgg attgttcctg
     3481 ctggcgacgg tcgctattgt tattcttgct gccttccccc aactgcgtcc gggttttgat
     3541 atcggtaaac cgatggaaac acgcgatatc attattatct gtatgctctc tgccgcctgc
     3601 ctgatggtgg tgctgtgtaa gacggcgacc gatgccatta ttctcacctc cacattccgt
     3661 gcggggatga gttcgctggc ggtaattctc ggcattgtaa ccttaggcac cacttttatt
     3721 gatgcgcatt taactgagat taaagatatt gctggtgata ttttgcaggc gtatccgatg
     3781 ttgttggcgc tggttctttt tggcacttgc gcgctgctct actcgcaagg tgcgaccacg
     3841 ccgttaatta tcccgcttgc cgtcgcgctt gacgtgccca cctgggcaat tctggcgtcc
     3901 tatgtggccg tcaccggagt atttgtactg ccaacgtatc caacatcact ggctgccatg
     3961 gaatttgata ctaccggcac aactcgtgta ggcaaatatg tgctgaatca tccatttatg
     4021 ctgcctgggt tggggggaat tattgctgga gtggcattag gctttgtgat tgcgccaatg
     4081 attatttagc actatcaaac atcagtatcg atggtttata cacaataaag acagatatgg
     4141 cggaatatag tctgttgtaa gcgccaacaa agaatagggg aattccgcca tgtctgaaca
     4201 tcttcacaat atggtattaa gtgttgtcgt ttaatgattt atttcttggt tatgttgtgt
     4261 attagctgca tctatcgctt tttgatacct gaggtgtact ggcaatagcg gacactacca
     4321 tttgttcttt ttttaagcag tcatctgatg atatttttcc ctgaaggctg ccg
//
LOCUS       DAETQL010000197         4209 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.201, whole genome shotgun sequence.
ACCESSION   DAETQL010000197 DAETQL010000000
VERSION     DAETQL010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.201"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="K0W65_004154"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="K0W65_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBI3097178.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="K0W65_004155"
     tRNA            complement(324..399)
                     /locus_tag="K0W65_004155"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="K0W65_004156"
     tRNA            complement(406..480)
                     /locus_tag="K0W65_004156"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="K0W65_004157"
     tRNA            complement(597..681)
                     /locus_tag="K0W65_004157"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="K0W65_004158"
     tRNA            complement(690..765)
                     /locus_tag="K0W65_004158"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="K0W65_004159"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="K0W65_004159"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBI3097179.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="K0W65_004160"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="K0W65_004160"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBI3097180.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="K0W65_004161"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="K0W65_004161"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBI3097181.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAETQL010000198         4185 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.202, whole genome shotgun sequence.
ACCESSION   DAETQL010000198 DAETQL010000000
VERSION     DAETQL010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.202"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(350..997)
                     /gene="ychE"
                     /locus_tag="K0W65_004162"
     CDS             complement(350..997)
                     /gene="ychE"
                     /locus_tag="K0W65_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBI3097182.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1474..4149
                     /gene="adhE"
                     /locus_tag="K0W65_004163"
                     /gene_synonym="adhC"
     CDS             1474..4149
                     /gene="adhE"
                     /locus_tag="K0W65_004163"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBI3097183.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1169 a         1021 c          959 g         1036 t
ORIGIN      
        1 tcaattcaat caaatagaga ttggctaagc atgtctatct tttctgatta cagttccagt
       61 agtgaaatgc acaataattt aacaattgat tattatttag cactttcctc taccaaaggc
      121 agtggaatta ctaatatcat ttcaattatt cttcagcaag cccaggatta tgatgtggcg
      181 aaaataacat gatggtgagg agaagcagtt aaggaaatcc ttataactaa ctgtaatttg
      241 agaagaatat ttttacctat tccttttgct tttgataact gcgcgggaac aaagtaaaaa
      301 taatgacgaa gtaaacggag catggcagct ccgtttcatt tgaaaggaat taattaagca
      361 ggccggggaa aatcccctta ataccagtaa cgataaattc aatccccaat gccatcagca
      421 atagccccat aatacgcgta atcacgttga tgccggtctg gcgtaaaacc cgtaccagcc
      481 acggtgccat gcggaacaat ccccagcaac ataaagcgaa caatgcaata gccacaaaga
      541 aaccaaacag atagctaatg ctgtgataac gcgtacccca gacgatggta gaactgatcg
      601 cccctggccc cgccatcaac ggcaacgcca atggcaccac accaatactt tcacgtaccg
      661 cggtttctga tttttcttgc ttgttctgtt tatcctcgcc aagcttgccg ctgatcatcg
      721 acatcgctat cgtcaccacc aggatgcccc cggcgatacg gaacgaatca attgatatac
      781 caaaaagttg tagaatcgtg tcgccgagaa aaagcgagat ccacaagata atggccacag
      841 acaggttggc tgtaaggtta gttttgtttc gcgctgccgc tgtctgataa ctggtcatgc
      901 tgataaagac gggaataatc cctaccgggt tgaccagcgc aaataacccg atgaaaaatt
      961 tgaagtaaac gggaaaatca aaaagggtct gaatcacggt tcgctccgaa gcaaaagccg
     1021 gataatgtta gccataaata aggttgaaaa gacgcgctga caatacgcct tttgacagca
     1081 tttttcacct cctaactact taaaattgct atcattcgtt attgttatct agttgtgcaa
     1141 aacatgctaa tgtagccacc aaatcatact acaatttatt aactgttagc tataatggcg
     1201 aaaagcgatg ctgaaaggtg tcagctttgc aaaaatttga tttggatcac gtaatcagta
     1261 cccagaagtg agtaatcttg cttacgccac ctggaagtga cgcattagag ataataactc
     1321 taatgtttaa actcttttag taaatcacag tgagtgtgag cgcgagtaag cttttgattt
     1381 tcattggtta agcaaatcat caccgcactg actatactct cgtattcgag cagatgattt
     1441 actaaaaaag tttaacatta tcaggagagc attatggctg ttactaatgt cgctgaactt
     1501 aacgcactcg tagagcgtgt aaaaaaagcc cagcgtgaat atgccagttt cactcaagag
     1561 caagtagaca aaatcttccg cgccgccgct ctggctgctg cagatgctcg aatcccactc
     1621 gcgaaaatgg ccgttgccga atccggcatg ggtatcgtcg aagataaagt gatcaaaaac
     1681 cactttgctt ctgaatatat ctacaacgcc tataaagatg aaaaaacctg tggtgttctg
     1741 tctgaagacg acacttttgg taccatcact atcgctgaac caatcggtat tatttgcggt
     1801 atcgttccga ccactaaccc gacttcaact gctatcttca aatcgctgat cagcctgaag
     1861 acccgtaacg ccattatctt ctccccgcac ccacgtgcaa aagatgcaac caacaaagcg
     1921 gctgatatcg ttctgcaggc tgctatcgct gccggtgctc cgaaagatct gatcggctgg
     1981 atcgatcaac cttctgttga actgtctaac gcactgatgc accacccaga catcaacctg
     2041 atcctcgcga ctggtggtcc gggcatggtt aaagccgcat acagctccgg taaaccagct
     2101 atcggtgtag gcgcgggtaa cactccagtt gttattgatg aaactgctga tatcaaacgt
     2161 gcagttgcat ctgtactgat gtccaaaacc ttcgacaacg gcgtaatctg tgcttctgaa
     2221 cagtctgttg ttgttgttga ctctgtttat gacgcagtac gtgaacgttt cgcaacccac
     2281 ggcggctatc tgttgcaggg taaagagctg aaagctgttc aggatgttat cctgaaaaat
     2341 ggtgcgctga acgcggctat cgttggtcag ccagcctata aaattgctga actggcaggc
     2401 ttctctgtac cagaaaacac caagattctg atcggtgaag tgaccgttgt tgatgaaagc
     2461 gaaccgttcg cacatgaaaa actgtccccg actctggcaa tgtaccgcgc taaagatttc
     2521 gaagacgcgg tagaaaaagc agagaaactg gttgctatgg gcggtatcgg tcatacctct
     2581 tgcctgtaca ctgaccagga taaccaaccg gctcgcgttt cttacttcgg tcagaaaatg
     2641 aaaacagctc gtatcctgat taacaccccg gcttctcagg gtggtatcgg tgacctgtat
     2701 aacttcaaac tcgcaccttc cctgactctg ggttgtggtt cctggggtgg taactccatc
     2761 tctgaaaacg ttggtccgaa acacctgatc aacaagaaaa ccgttgctaa gcgagctgaa
     2821 aacatgttgt ggcacaaact tccgaaatct atctacttcc gccgtggctc cctgccaatc
     2881 gcgctggatg aagtgattac tgatggccac aaacgtgcgc tcatcgtgac tgaccgcttc
     2941 ctgttcaaca atggttatgc tgatcagatc acttccgtac tgaaagcagc aggcgttgaa
     3001 actgaagtct tcttcgaagt agaagcggac ccgaccctga gcatcgttcg taaaggtgca
     3061 gaactggcaa actccttcaa accagacgtg attatcgcgc tgggtggtgg ttccccgatg
     3121 gacgccgcga agatcatgtg ggttatgtac gaacatccgg aaactcactt cgaagagctg
     3181 gcgctgcgct ttatggatat ccgtaaacgt atctacaagt tcccgaaaat gggcgtgaaa
     3241 gcgaaaatga tcgctgtcac caccacttct ggtacaggtt ctgaagtcac tccgtttgcg
     3301 gttgtaactg acgacgctac tggtcagaaa tacccgctgg cggactatgc gctgaccccg
     3361 gatatggcga ttgtcgacgc caacctggtt atggacatgc cgaagtccct gtgtgctttc
     3421 ggtggtctgg acgcagtaac tcacgccatg gaagcttatg tttctgtact ggcatctgag
     3481 ttctctgatg gtcaggctct gcaggcactg aaactgctga aagaatatct gccagcgtcc
     3541 taccacgaag ggtctaaaaa tccggtagcg cgtgaacgtg ttcacagtgc agcaactatc
     3601 gcgggtatcg catttgcgaa cgccttcctg ggtgtatgtc actcaatggc gcacaaactg
     3661 ggttcccagt tccatattcc gcacggtctg gcaaacgccc tgctgatttg taacgttatt
     3721 cgctacaatg cgaacgacaa cccgaccaag cagactgcat tcagccagta tgaccgtccg
     3781 caggctcgcc gtcgttatgc tgaaattgcc gaccacctgg gtctgagcgc accgggcgac
     3841 cgtactgctg ctaagatcga gaaactgctg gcatggctgg aaacgctgaa agctgaactg
     3901 ggtattccga aatctatccg tgaagctggc gttcaggaag cagacttcct ggcgaacgtg
     3961 gataaactgt ctgaagatgc attcgatgac cagtgcaccg gcgctaaccc gcgttacccg
     4021 ctgatctccg agctgaaaca gattctgctg gatacctact acggtcgtga ttatgtagaa
     4081 gatgaaactg cagcgaaaaa agaagccgct ccggctaaag ctgagaaaaa agcgaaaaaa
     4141 tccgcttaat cagtagcgct gtctggcaat ataaacggcc ccttc
//
LOCUS       DAETQL010000199         4168 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.203, whole genome shotgun sequence.
ACCESSION   DAETQL010000199 DAETQL010000000
VERSION     DAETQL010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.203"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..334)
                     /gene="flhe"
                     /locus_tag="K0W65_004164"
     CDS             complement(<1..334)
                     /gene="flhe"
                     /locus_tag="K0W65_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HBI3097184.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(334..2411)
                     /gene="flhA"
                     /locus_tag="K0W65_004165"
                     /pseudo
     CDS             complement(334..2411)
                     /gene="flhA"
                     /locus_tag="K0W65_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="K0W65_004166"
                     /pseudo
     CDS             complement(2404..3551)
                     /gene="flhB"
                     /locus_tag="K0W65_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            3787..>4168
                     /locus_tag="K0W65_004167"
     CDS             3787..>4168
                     /locus_tag="K0W65_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBI3097185.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT         1038 a         1208 c         1021 g          901 t
ORIGIN      
        1 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
       61 ccacggtggt accgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
      121 cccgcagtcc tgcaggtgtc gggccgataa gctgataacg ccacgccacc agcgtcatca
      181 atcctgaagc aggttgtcgc gcagaaagag gtgcagacga catcgactca ccgcgatgat
      241 ttagcgtaat tccgacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
      301 gcggaaacaa taatatcgct aataaggttc tcatcattta ccgccaattg tcgccgtcat
      361 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
      421 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
      481 cgcacccagc atctcctgac gggatagagc ttcctgagtt tgcgccagta aacgatccgc
      541 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac gttccagcgg
      601 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
      661 ccgtcccaac gccacgcgca cgacggcgct taattcatgt ggatcgcttt ggatgggcgc
      721 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
      781 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
      841 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
      901 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
      961 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
     1021 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
     1081 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
     1141 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
     1201 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
     1261 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
     1321 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
     1381 tgccattttt accggtttgg gttcggcagg cgctttttgt tcgcgtccgc gtatccacca
     1441 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
     1501 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
     1561 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgccccc
     1621 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
     1681 tagcgtataa ctttccgccg cgtgtcccat gctcatgcca gttgcagcac gccgaccagc
     1741 aacccgccga caacgttaat gaccatgatg aggatcccgg caatggcatc gccgcgaaca
     1801 aacttacttg ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg
     1861 cgttttttcg cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc
     1921 tgcttacccg gcataccatc gagaacaaag cgcgcaccca cttctgctat acgcccggca
     1981 cctttggtaa tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata
     2041 gcgaaattgc caccaacgag gaagtgaccg aacgcttcga ccaccttccc agccgccgcc
     2101 gcgccggtat gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc
     2161 aacaacatag taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta
     2221 aacatcgcca ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg
     2281 agtatgaatg cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt
     2341 ccggcaagga tctgccatta tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc
     2401 agattactca tgggtcggtt tctcgttaat aaaatccagg gctgccggca ccggaagatg
     2461 agtaggtgta cagggcgctg tccaccagcc agacgccagc gtttcagttg ccagacccag
     2521 gccagcactt ccgccaccgc ggcgtacagt tgacccggga tttgttgacc aatctccgca
     2581 tgtcgataca gcgctcgcgc cagcggcggc gcttcaagcg tcgggacgtt attttcagcg
     2641 ccaatttcac gaatgcgcag cgcgaccagc cctgcacctt tagcgaccac tttcggtgcg
     2701 ctcattttgt tttcgtcata ctgcaacgct accgaatagt gggtcgggtt attgacaatg
     2761 acatccgctt tcggcacatc ggccatcatc cgacgccgtg cggcagctcg ctgcatctga
     2821 cagatccgtc ctttaacatg ggggtcgcct tcgctttgtt tgaactcatc acgaatatcc
     2881 tgccgcgaca ttcgcagctt tttcaggtgg ctgaagattt ggaaaaagac gtcaaatccc
     2941 accattggaa tgacaccaag caccaccagc agtgcgcata gccctaccaa atccatcgca
     3001 ttacccatgg cggtaatcgg tgactcggcc attaagcgca tcatctgcgg ccagtgatgc
     3061 cagagaaaaa accccgtcac gctgccaacc aggatggttt tcaaaattgc cttaagcaac
     3121 tccgcgctag tctgtgccga gaatatccgt ttaatgcccg gtagcgggtt gagtttggaa
     3181 aacttcggct gcaaggattt gccgctaaat accagccccc ccagcataac cggagaaatg
     3241 agcgccacca gcaccacgcc gctaatcagc ggtagcagcg ccagcatggc ttctctgatc
     3301 agcagaataa tttgcccgag gatcagattc gggtctttga taatactgtg atcaaaatgc
     3361 agcccagcgg agagcatgcc cgacaatcga cgggccagcg acacaccgcc aaaccagata
     3421 acactaacgc ccaccagcaa aatcagcagt gaggtcagtt cacgggaacg cgggatttgc
     3481 ccctcttccc gcgctttttc tagtcggtga ggtgtggggg cttctgtttt gtcgtcgctc
     3541 tcgtcagaca cgtcgccaat cctggaaaga gtattaagcg taaatgatgc cagagcgcaa
     3601 agcgttcaat ggtttaagta aggggcaaaa caggcgggat ttagggcgtt tatgcaaaaa
     3661 taggttcagt tgcagaacgt tttgcgaacc attttccagt tctgatattt agccatttag
     3721 cctatagaat cgcactccgc ctctctgtta gtttgctaat aattaaatta ttacaaaggt
     3781 gatcaaatgg aatggcaaca attgcttaac tgcaaccgtc ggaaagataa tctaaaaaaa
     3841 accaacatag catttttaca tccccttgct agcggaagac aagagatcga gcgggattac
     3901 gaccgattac tttttgctgc accaactcgt cggcttgcgg ataaaacaca agttttcccg
     3961 ttagatcaaa atgacagcat acggacgagg ctgactcatt ctcacgaagt agcaaacttt
     4021 gctcgcggga taggaatgag gctcgctttc gagatgaagg agcacatctt tggcgaaata
     4081 cccacgggga ttgtcgttga acgcgacgtt cctgctctgt tagcagcgat cggactggca
     4141 cacgatctcg gtaatccacc atttgggc
//
LOCUS       DAETQL010000200         4114 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.204, whole genome shotgun sequence.
ACCESSION   DAETQL010000200 DAETQL010000000
VERSION     DAETQL010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.204"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..158)
                     /locus_tag="K0W65_004168"
     CDS             complement(<1..158)
                     /locus_tag="K0W65_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBI3097186.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="K0W65_004169"
     CDS             complement(308..469)
                     /locus_tag="K0W65_004169"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097187.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..654)
                     /locus_tag="K0W65_004170"
     CDS             complement(466..654)
                     /locus_tag="K0W65_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBI3097188.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="K0W65_004171"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="K0W65_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBI3097189.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="K0W65_004172"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="K0W65_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="K0W65_004173"
     tRNA            complement(2161..2237)
                     /locus_tag="K0W65_004173"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="K0W65_004174"
     tRNA            complement(2339..2414)
                     /locus_tag="K0W65_004174"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="K0W65_004175"
     CDS             complement(2559..3380)
                     /locus_tag="K0W65_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBI3097190.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="K0W65_004176"
     CDS             complement(3395..3751)
                     /locus_tag="K0W65_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBI3097191.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4114)
                     /locus_tag="K0W65_004177"
     CDS             complement(3764..>4114)
                     /locus_tag="K0W65_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBI3097192.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1154 a         1118 c          794 g         1048 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatgtt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaaaacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgg
//
LOCUS       DAETQL010000201         4043 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.205, whole genome shotgun sequence.
ACCESSION   DAETQL010000201 DAETQL010000000
VERSION     DAETQL010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.205"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(887..1078)
                     /locus_tag="K0W65_004178"
     CDS             complement(887..1078)
                     /locus_tag="K0W65_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBI3097193.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENR"
     gene            1143..1466
                     /locus_tag="K0W65_004179"
     CDS             1143..1466
                     /locus_tag="K0W65_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBI3097194.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1408..2166
                     /locus_tag="K0W65_004180"
                     /pseudo
     CDS             1408..2166
                     /locus_tag="K0W65_004180"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2144..2674
                     /locus_tag="K0W65_004181"
     CDS             2144..2674
                     /locus_tag="K0W65_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HBI3097195.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2681..2914
                     /locus_tag="K0W65_004182"
     CDS             2681..2914
                     /locus_tag="K0W65_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HBI3097196.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3460..3852)
                     /locus_tag="K0W65_004183"
     CDS             complement(3460..3852)
                     /locus_tag="K0W65_004183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097197.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          918 c         1207 g          952 t
ORIGIN      
        1 aaaaaaaacc accgctacca acggtggttt gtttgccgga atctagagct accaactctt
       61 tcttccgaag gtaactggct tggtggagca cagataccaa atgctgtcct tctagtgtag
      121 ccgcagttag gccaccactt caagaactct taatatctca atccaccttg tccagttacc
      181 aatggctgct gccagtggcg ttaagtcgtg tcttaccggg ttggactcaa gacgatagtt
      241 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      301 gcgaacgacc tacaccgaac tgagatacca acagcgtgag ctatgagaaa gcgccacgct
      361 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      421 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      481 cctctgactt gagcgtcggt ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      541 cgccatcggc gcggccttct tcctgttcct ggtcttttgc tcacatgttc tttccggcgt
      601 tatcccctga ttctgtggat aacagcattt cgtttttgag tgagtagata ccgctcgccg
      661 cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaaaagc gcctggacgt
      721 gcattttctc cttacgcatc tgtgcggcat ttcacacccg gtatggcgca cttttcatac
      781 aatccgcact gatgccgcat ggttaagcca gtatacactc cgctatcgct acgtgactgg
      841 gtcatggctg cgccccgaca cccgctaaca cccgctgaca agcccgtcag cggttttcac
      901 cgtcatcccc gaagcgagcg aggcaactgc ggtaaagctc atcggcgtgg tcgtaaagcg
      961 attcacagat gtctgcctgt tcatccgcgt ccagttcgtt gagcttctcc agcagcgtta
     1021 atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg
     1081 atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgcgaga
     1141 ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgctgtgagg
     1201 gtaaacagct ggcggtatgg atgcggcggg tctgcctggg tgagccggtt gcccgttccg
     1261 ggaggctgcc gacactggca ccgccgttac tgcgtcagct ggccgccatc gggaataacc
     1321 tgaatcaggc agcgaggaag gtgaacagcg ggcagtggtc ttccggtgac cgggttcagg
     1381 tggtggccgc actgatggcc atcggggatg agctgcgccg gctgcgtctg gccgtcaggg
     1441 aacaggggac gcgggatgat agttaagttt catgccaggg gaaaaggcgg tggcagtggt
     1501 ccggttgatt acctgctggg aagggagcgt aaccgggaag gggcaacggt gcttcagggt
     1561 aatccggaag aagtccggga actcatcgat gccacgccat ttgcgaagaa atacacgtcc
     1621 ggtgttctct cgttcgcgga gaaggagctg ccgccgggag ggcgtgaaaa agtgatggcg
     1681 agctttgagc gtgtactgat gcccggtctc gaaaaaaatc agtacagcat tctgtgggtg
     1741 gagcaccagg acaaggggcg gcttgagctg aattttgtca ttccgaacat ggagctacag
     1801 accggaaaac gcctccagcc gtactacgac cgcgcagaca ggccgagaat tgatgcctgg
     1861 cagacgctgg tgaatcacca ttacgggctg catgacccga acgccccgga gaaccgccgg
     1921 acgctgactc tccctgataa cctgccggaa acgaaacagg cgcttgctga gagcattaca
     1981 cggtggatag acgctcttta tcatgtcggg gagataaaag gccgtcagga tgtgattcag
     2041 gcgctcacag aggcggggct tgaagtggtc agggtgacgc gaagcagtat cagcattgca
     2101 gacccgaacg gcgggaagaa tatcagactg aaaggagcat tctatgagca atcttttaca
     2161 gacgggcgcg gagttcgaga aaaagctgaa agagagagcc gaatctacag agaaaatgct
     2221 gagcgacgag ttcaggaagc tcgaagaatc tgtaagcaag gctgtgacat caaacgagac
     2281 gaaaatcaaa gacgctatag ccctgttcac agcttcgaca gaggaatcac tgaaaaaaca
     2341 tcgggaaggg gtgaaagagg cgatgatgca gcacaggaag gacgtgttaa agctggcagg
     2401 gaatacgggc atgatgttac tggggatggt cattttcctg tttaccgtga gtggcgggac
     2461 gctctggtat cttggcggga tgatacaggc gaacctggaa gaaatcagga agcagaacga
     2521 gagtctggag aagctgaacg cgaagacatg gggcgtgcgt tatcacgagg ggagcaacgg
     2581 acgttttctg gtgttgcccg aggggatgaa agccgaaacg aactggacga tggaaaacgg
     2641 gaaacagaat gccgtaaggc tggtacagga gtgacagagc atgacggagc tggaaagcca
     2701 tttgctgaac gcattagagc agctgcaaca ggactatatg cagcggctga acgaatggga
     2761 gagcgccttc gtggaattgc agaaaatgtt ttcgcttacg caacgggaca acgcgatgct
     2821 gaacgagcgg gtcatgcagt tgagtcagca ggtgctgcac ttgagcgagc agacagaacg
     2881 cttgagccag ttatacagcg agaactggag ataagagagg aacggttgat acaggagcgc
     2941 gaacatgcgt tattcctgga acgggaacgc cagccggaaa tacaggaacg gacactggat
     3001 ggcccttcgc tgggatggta atcagagaca ggctaaaatg ttgtcagcgc gtttccggtg
     3061 cattttttgg gataatggtt ggttcggggg caccgggaac gcgcttcagt cagaccttgc
     3121 tgaaaacaca ggaacgagtt aaaacgcata cgggcgcgat tctggtgtgt cttttgtcgc
     3181 tgtgttttgc ctctattgcc agatcgcgct cattcccctg aacttctgtt ttcccgctca
     3241 ggtccccgtc ttcttcgctt ccgaaagcat gaataaaatc cgtttcagct gtctctggct
     3301 ggcaattcct gcgtgattca catggctgca tagctatgca tgtggttaaa atttaccggg
     3361 tgcgatcgcg gcagtttttc gggtggtttg ttgccatttt tacctgtctg ctgccgtgat
     3421 cgcgccgaac gcgtttcagc ggtgcgtaca attaagatat tacgttaaat tgtgagggaa
     3481 tggtccccat atactgactt tatcataggg atagggttgg tagtgggtcg attgggtttc
     3541 ggcattgttt ccgttaatgc caattgcatt tttgtatttg atgcaacaga aggcgtgaga
     3601 ttctcctggg cctggaaagg ctaagtgttt agaattgatg gcaaaatatc ttccgtccga
     3661 tttttgaagt agttgttgtt tggctgtctc ccaaagggtg ctgttacaaa cttcttgaat
     3721 ttgtagtttt tgcaggactc ttattactgc gttgtcatgt tccaattctt taggggagtg
     3781 tattattctc tcttttaata agctgtttac cacagggttt agttgttgtc taaagcacgc
     3841 agataatgcc attgctacac aattttgttc ccgaggaagc atgtttggcg gggtatgccg
     3901 tgtttgatgt gatatgtcta ttctcataga aggtctctgg cttatagcat caacaaatac
     3961 ggctataaaa caaaattttc atagagtgat tcaagatctt cttgagatcc ttttttgcgg
     4021 cgcgtaatct gctgcctgca aac
//
LOCUS       DAETQL010000202         3885 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.206, whole genome shotgun sequence.
ACCESSION   DAETQL010000202 DAETQL010000000
VERSION     DAETQL010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.206"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(280..483)
                     /locus_tag="K0W65_004184"
     CDS             complement(280..483)
                     /locus_tag="K0W65_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097198.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(485..640)
                     /locus_tag="K0W65_004185"
     CDS             complement(485..640)
                     /locus_tag="K0W65_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HBI3097199.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(843..1250)
                     /locus_tag="K0W65_004186"
     CDS             complement(843..1250)
                     /locus_tag="K0W65_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3097200.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1327..1554
                     /locus_tag="K0W65_004187"
     CDS             1327..1554
                     /locus_tag="K0W65_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3097201.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1538..1960
                     /locus_tag="K0W65_004188"
     CDS             1538..1960
                     /locus_tag="K0W65_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097202.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            2038..2815
                     /locus_tag="K0W65_004189"
                     /pseudo
     CDS             2038..2815
                     /locus_tag="K0W65_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2822..3568
                     /locus_tag="K0W65_004190"
                     /pseudo
     CDS             2822..3568
                     /locus_tag="K0W65_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            3583..>3885
                     /locus_tag="K0W65_004191"
     CDS             3583..>3885
                     /locus_tag="K0W65_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBI3097203.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPD"
BASE COUNT          997 a          850 c         1062 g          976 t
ORIGIN      
        1 gtaatgggtt aagtgataac agatgtctgg aaatataggg gcaaatccac tgagccagag
       61 gattacgaag ggcttcataa atatatgctc tcactaggat tcaggagaac gatcccccat
      121 ggagatggtt cgtataatca actcccggac ggaacttatg tttccgaaaa gggcggtgat
      181 atttatcaaa ttcgcagcca gatatctgac tatgcagacc gactatccgg gtatcgcgcg
      241 tctgtttttg tttgcgaatt cagtcaatgc gcatggtatt tataccccgc caagacccgg
      301 tgaatatcct gcacggcctt ctgctttgta gaaggcttct tcgctatcat aatcgccaga
      361 ttccagcgct tctttagcca gccagatgcg tgccccagtg cctgcatttg gctccagttg
      421 ctggaggcgt tttgcatctt ctaggagtag agcgataacg tgttttaatt ctgtctcgtt
      481 cattttactc acctgaatgt cttcccaacc aacgacgtgc gccagcttcg gttttaaacg
      541 ttttgctttt ggtatacgtt atggcggtga atgtgccgtc ctggttgggg aacacgccgt
      601 acaccggaga ttcgttgttg ccaagatcga tagtattcat gttgacccca tttcccctta
      661 acgccggggt agcggaactg tttgctgaga acaccgtgcg gtgtcttgat gaataaaatt
      721 tagaataacc taaggagggt gatcaagatt tttgtgtaga aaaacctaag ttttttgatg
      781 taaaaaacac aagtgtttga aagtttgtgc tttttattac agggtgtgga gaaaaaaggg
      841 gattatttgt ttgcgcttct tttgcgagct ttgagtagtt cttcaaaaag tttgttgaaa
      901 ttttcaactc gagcacgcat ctctgacaac agggcctttt gctctgactc aggcagtgcg
      961 tcgaacagtt gaagtaactc tttttgatct tctgtcagaa tggctggctg attatccggg
     1021 atcggttcgc ctggttgttt atcttcatcc ccaaaaagaa gccaagtcgg tgagcactga
     1081 agcgcttggc tcagtgcgaa taatcttttc cccgccggct gtgtttcatc tctttcccat
     1141 tgagaaattg ttacgtgagc cactttgacc agcttaccta atgcggcctg agacagtttt
     1201 aattttttac gcctatgtaa gaggcgagca ccgaaggttt cgtttttcat attagggaat
     1261 tctaattttt cttgacttag gtttctctac ggcctggttt ccttaggaaa atctaaggag
     1321 ctcgatatgt tgaaaattga tgctatagcg ttttttggca gcaaaacaaa gcttgccaat
     1381 gccgcaggag ttaggctggc aagcattgct gcatgggggg aactggttcc tgaaggtcgc
     1441 gcgatgcgcc tgcaagaggc atctggcggg gaacttcagt acgaccccaa agtttatgac
     1501 gaatatcgta aggcaaaacg acctgggaag gtgattcatg aaaatcaggc atgagcacat
     1561 cgaatcagtg ctgttagccc tggcagccga aaaagggcag gcgtgggtcg ctaacgcaat
     1621 tactgaagaa tatctgcggc aggggggcgg cgaattgtct ctggtaccag gcaaggactg
     1681 gaacaatcag cagaatatct atcaccgttg gttgaaaggt gaaacgaaag cgcaaaggga
     1741 aaaaattcag aaactgatcc ctgcggttct ggcaattctt ccgcgtgagc tgcgtcaccg
     1801 actctgtatc ttcgataccc tggaacgccg tgcattactg gcggcgcagg aagcgttgag
     1861 tacggcaatt gatgcgcatg atgatgctgt ccaggccgtt taccggaaag cacatttcag
     1921 cggtggtggg tcgcccggcg attctgtcgt agtgcattga ttgaaattaa tcgtgccgga
     1981 ttgttttgtt cggtatcagt taaatgtaac gctgcgagcg ttacaaggtg aaaacaaatg
     2041 gcttcaaact ggataaagct cgaggttatt acgccggata agccggaaat attcaggctt
     2101 gctgagattc tgaatattga tccagatgcc gcattaggga aggttattcg cttctgggca
     2161 tgggcggatc aacaaatgat agacggtaat gcagattgta acgctcgcgg cgttacaaaa
     2221 agtgcaatag atcgcatcac ttttatgtct ggttttgctg atgcgttaat tcaggttgga
     2281 tggctggtcg aaaatgacgg tgggctttct ctacctaact ttgaacgtca taacggaaaa
     2341 agctctaaaa aacgggcggt tacaaacgag cgagtaacaa aaatacgcga actgaaacga
     2401 aaaggtaacg ctgccagcgt tacacaaacg gatcaaaaag cgttaccagt ggaagaggaa
     2461 gaggaagatc taaatactga tctcccccta aatccccctc gccaaaaacg agcgtctaaa
     2521 aaattcgagc cggaggctat tgagctgccc gattggttgc cggaaacact ctggcatgag
     2581 tgggtccggt tcagacaggc attgcgaaaa ccgattcgaa cggagcaggg cgatacggga
     2641 actggaaaaa ttccgtcagc agggttttac acctgagcag gtgattcgac acagcatcgc
     2701 caatgaatac cagggcctgt tcgcgccgaa aggtgttcgg cctgagacgt tgctccgaca
     2761 ggttaacacc gtctcgttgc cggacagtgc gatcccgcca ggcttcaggg ggtaacggac
     2821 catgaaaaat attgcgacag gcggcgttct ggaacgcatc cgccgactga ccccgcaaca
     2881 tgtaaccgcc ccattcagaa cggtagcgga gtggcgcgag tggcaacttg ctgaaggcca
     2941 gaaacgtagc gaggagatca accgcctgaa tcgccagttg cgggtggaaa aaattctgaa
     3001 tcgctcaggc atccagccgt tgcaccgtaa atgctcgttt gcgaattacc aggtgcagaa
     3061 cgacggccag cgatacgcgt taagccaggc gaaatccatc gccgatgaac tgatgaccgg
     3121 gtgtacaaat tttgcgttca gcggaaaacc tgataccggg tagaatcact tagcggcagc
     3181 tatcgggaat cgcctgctga aagacggtca gacagtgatt gtggttaccg tggctgatgt
     3241 tatgagcgcc ctgcacgcca gctatgacga cgggcagtca ggcgaaaaat ttttgcggga
     3301 gctgtgcgaa gtggatctgc tggttcttga tgaaattggc attcagcgcg agacgaaaaa
     3361 cgagcaggtg gtgctgcacc agattgttga tcgccggaca gcgtcgatgc gcagcgtggg
     3421 gatgctgaca aatattaacc atgcagcgat gaatacactt ctcggcgagc gggtgatgga
     3481 tcgcatggtc atgaacggcg ggcgctgggt gaattttaac tgggagagct ggcgtccgaa
     3541 tgttagccat tcgagggttg ttaagtagtt tcaggaggat ttatggcgaa accttttact
     3601 cccgaacagc gggaagaact gaagacgcga attgtggaac tcgtgcatca ggacggtcgg
     3661 gtcacgattc ggcagttgtc cgatgaaaca ggtatcagtc gtgcgtctgt cggtcgctta
     3721 tgcatagaac tggtcgcaag tggtgatgta tataattctg gctacggctt attcccgtct
     3781 gaacaggctc gcaaggactg gcaaagcgcc cgcaaaaaac tctcgagagt aaaggtgagg
     3841 aaaccggttg ttgttgatcc ggaccttatc tggtcattac ctgac
//
LOCUS       DAETQL010000203         3701 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.207, whole genome shotgun sequence.
ACCESSION   DAETQL010000203 DAETQL010000000
VERSION     DAETQL010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3701
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.207"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..451
                     /locus_tag="K0W65_004192"
                     /pseudo
     CDS             1..451
                     /locus_tag="K0W65_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            481..884
                     /locus_tag="K0W65_004193"
                     /pseudo
     CDS             481..884
                     /locus_tag="K0W65_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1469..2185
                     /locus_tag="K0W65_004194"
     CDS             1469..2185
                     /locus_tag="K0W65_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097204.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2737..3141
                     /locus_tag="K0W65_004195"
     CDS             2737..3141
                     /locus_tag="K0W65_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097205.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            3204..3701
                     /locus_tag="K0W65_004196"
                     /pseudo
     CDS             3204..3701
                     /locus_tag="K0W65_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1044 a          771 c          884 g         1002 t
ORIGIN      
        1 atgaacatcc gcatcttcct gttgaaagtc gtcgtcaggc aaagggaact cgcttcctgt
       61 ggcagcacag cgataaatgg atgaccgaat ccaggcagga aaagctgata tggctgcgtg
      121 cacagatgaa gctgacgagc ctgtgctggg cgctgaaaga actggcaaag gatatctgga
      181 gcagaccatg gagcgaggaa agacgcaacg actggcagag atggctgcgg ccaacagtga
      241 cgtccccatg atgaagaatg tggctaaaac gatagggaaa aggttgtacg gtatcctgaa
      301 cgccatgcgc catggcgtat caaacggaaa tgcggaagca ctgaacagca agatcaggct
      361 gctgagaata aaagccaagg gctaccgcaa ccgggagcgc tttaagctgg gagtgatgtt
      421 ccactatggg aaactgaata tagcgttcta agccttccca ccatgatcgg ggaagcccca
      481 gatatgaaca ggcacattca gagttacttt aaagagctta ctgaacgtct gaggtgttag
      541 agccatgact gccgcagcat tgctggcgga aatgcctgaa tcgagcagcc tctcgagacg
      601 tgaaatcagc gcacttgtcg gtgttgctca ggtaaaccgg gactcgggaa ctctgcgcgg
      661 tcgacgaacc attttcggtg attgtgctgg ggaagaacag ctctgtacat ggcggcgctt
      721 gaggcccccc ccggtttaat ctggtgataa aggcgtttta catgcgccta cttgcagccg
      781 gaaacgccaa aaaagtggca ctggttgcct gtatgcgtaa gttgctgacc attatgaacg
      841 caatgctcag aaaaaacgaa gaatggaatg aatcgtattt atgaaatact ggattgtggg
      901 ccataaatat tccaaaaaga tagcccgctc cgtttattac cggaacgggc gagagcatga
      961 cattgtctgt tattataatg agactgctga ttctacttat ttttaatttc aagaaaagcg
     1021 ttttctacaa agaattatga gatttaatgt tttgtttaga caatattatc attctgttca
     1081 ataatgaaag tcttcatcaa caacaagtaa aaacaacgtc cctctcatcg aaccggactt
     1141 gcagatttcc cgcatccggc tctcttgcaa cacatcagac cttcacgttc gggcggttat
     1201 ggggaaggtc cagcaagctg tatgagccct aactgtttga ataccagtag attaccagaa
     1261 acttctatcg attgaagaca ttaccaacta gcgttactaa atgttgacca agttttgttc
     1321 acttgtctgg ttttgatagt agcattctgc agagtatttt gatatgataa atatgcatta
     1381 acaatgatat tattgttaag ttcctagcat gaaaattaaa agatcaactt ttatctcgaa
     1441 tattttttac attatcagtt agtttcttat gaatgacaat tcattgctta gaaattcttc
     1501 attattcatc gcctatatgg gatgtgtagg gtgggtcagt gcttattctt atggctgggg
     1561 aacctctttt tactatggat ttccatggtg ggttgttggt gcaggtttgg atgatgttgc
     1621 cagaagttta ctctatgcta tcatagtaat gggtatactt tttaccggtt gggggattgg
     1681 tattttattt tttcttctta taaagaaaag aagtaaaata caagacttga gtttttttag
     1741 actatttttt gctattaccc tactgttttt ccctgtaata ttcgaacttt taattctcaa
     1801 acaatatttc atacttccat tatcactttc attcattatt agttcattgg ttatttcaat
     1861 aataattcgt atatatggac ggatcttttc tgtatcatgt ttttcagata tcccttttgt
     1921 tagagaacac cgcattaagt taattatggc tggttttttg gtttattttt ggtttttttc
     1981 ctttcttgtt ggctggtata aacctcaatt aaaaaaagaa tatcagatgc tttgttataa
     2041 taatagttgg tattatgttc ttgctcgtta tgattcacgg ctcgttttat ctagttcatt
     2101 taaagatgac agtaacagat tcttaatatt taatacagaa cagtcgggat tttatgaaat
     2161 caatgatgtt tatgtcagaa agtaataatc ctctcccaca aagtgaatta aatgaatata
     2221 taatgcgttc gaagtatatg tattgagctg tcatactgag aaacaaacct tagggcagca
     2281 caattaaagc cagaaacaac tatgcaagaa agttactaaa taagtgctcg gaacagtaaa
     2341 acactcaggg gggactcagt ccggattatg taatcagata aattgccaaa catcacaaat
     2401 caccagccgg actgagtgat gccgagcatt gcaccaatac cccgtgatga acgatgcctg
     2461 atgcagaaag ctccctataa aacaaagaac aaaaactacg accggcgatt caccgtcata
     2521 ctgatgctgc atcgtggtga cactgtctgt cactttcatg ttgactgaac aactctgctg
     2581 cgaagaaggt gtaccgccgg cgggatttga ttgtccggcg tctagcttcc acttccctga
     2641 tggaactgaa agttgttatg gccggtcagc ttgccctgtc cgcaggacat gaggtgctgt
     2701 gacggacagc accagtaacg gaaagtgaca atgaaaatga atactgcgct gatagttgca
     2761 ctaatgtgta tgtggtatgc ggtacccgct gctgcaaaag agactttact ggctatgcca
     2821 cgtaattcaa cagaacattg ctatgcggag attaatgtgc acggaccata tggggtgtat
     2881 ttccgagtgg tcccgcatcc ccccggggga aagagctggg tcgaatgtaa cagtgactac
     2941 tattactctg ataaaccacc gggagtacag atactcggaa cccgtgcagg gtgtcgggta
     3001 tacggaatct gcgggaccac ctcgacactg catgtggctg gaagaggggt tgtgtgtatt
     3061 aaaaatatat gcagtccacg tgggatgata attcacagga tacggaaacg tcctgtagtg
     3121 gctgtaagcg atgagatgtg atcttaccca gcaatagtag acacgcggct aagtgagtaa
     3181 actctcagtc agaggtgact cacatgacaa aaacagtatc aaccagcaaa aaaacacgta
     3241 aacagcattc gcctgaattt tgcagtgaag ccctgaagct tgctgaacgc atcggtgttg
     3301 ctgccgcagc ccgtgaactc agcctgtatg aatctcagct ctatgcgtgg cgcagcaaac
     3361 tgcagcaaca gatgacctcg tctgagcgtg aaagtgaact gcccgcctga agcggcaact
     3421 ggcggagcag gcagaggaac tggctatcct ccaaaaggcc gcgacatact ttgcgcagcg
     3481 gctggtatac gtggtgtcag cgtcggacag gaataagccc tcgtcagcag ttccgtcagc
     3541 actgcgacag cgttgtcctc gcggcggcct ttacccggtc aaaacagcgt tacggtgcac
     3601 cacgcctgac ggatgaactg cgtgctcagg gttaccactt taacgtaaaa accgtggcgg
     3661 caagcctgcg ccgtcagggg ctgagggcaa aggcctcccg g
//
LOCUS       DAETQL010000204         3563 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.208, whole genome shotgun sequence.
ACCESSION   DAETQL010000204 DAETQL010000000
VERSION     DAETQL010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.208"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..66
                     /locus_tag="K0W65_004197"
     CDS             <1..66
                     /locus_tag="K0W65_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="with Hns involved in transcriptional regulation of
                     hemolysin; non-specific DNA-binding protein which affects
                     the production of multiple proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3097206.1"
                     /translation="AELITGKLYDRIPKEIWKYVR"
     gene            105..695
                     /locus_tag="K0W65_004198"
     CDS             105..695
                     /locus_tag="K0W65_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBI3097207.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            935..1195
                     /gene="repA"
                     /locus_tag="K0W65_004199"
     CDS             935..1195
                     /gene="repA"
                     /locus_tag="K0W65_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBI3097208.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1419..1493
                     /gene="tap"
                     /locus_tag="K0W65_004200"
     CDS             1419..1493
                     /gene="tap"
                     /locus_tag="K0W65_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBI3097209.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1486..2343
                     /gene="repA"
                     /locus_tag="K0W65_004201"
     CDS             1486..2343
                     /gene="repA"
                     /locus_tag="K0W65_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBI3097210.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3044..3235
                     /locus_tag="K0W65_004202"
     CDS             3044..3235
                     /locus_tag="K0W65_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097211.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          946 a          899 c          891 g          827 t
ORIGIN      
        1 gcggaattaa tcaccggaaa gttatatgac aggataccaa aagagatatg gaaatatgta
       61 aggtaataat tcagcaataa ttaattgcca ggagcagata aataatgaag ttgattattt
      121 ttattctcat tgttctgatt attgcagcgc tacttatccg gattatcctc agaagtgtaa
      181 atcagcattc tcctttgctg atgcaattgc atgcggcagg tatacggact ggtgatgcag
      241 agcgaatact gtccagcggt gaatactggc aacgtcagaa gacgctgctg acagaaaggg
      301 aagtcagttt tatgaaagga ctgttcagaa ttgtggatat gaagcggtgg tatctgtgtc
      361 cgcaggtacg ggtagcggat atcgtccagc tgaacgggaa tatccggcca cgatcacgcc
      421 agtggtggca gttattcagg atggtgtctc agtggcatgt tgatgtggtt atcgttgagc
      481 tgcgttcgtt cagtattgtt gcagcagtag agctggatga tgccagccat ttacgaccgg
      541 aacgcagacg ccgggatatt cttctggaag aggttctgag gcaggctggt attccgttgc
      601 tcagaagcca cgatgccaga aaactgctgc agatgaccgg agaatggctg aatacaacag
      661 gggctgatca gcagtcaccg gaacatcgta gctgacgcct tcgcgttgct cagttgtcca
      721 accccggaaa cgggaaaaag caagttttcc tcgctcccgg cgtttccata actgaaaacc
      781 atactatttc acagtttaaa tcacattaaa cgacagtaat ccccgttgat ttgtgcgcca
      841 acacagatct tcgccacaat tctcaagtcg ctgatttcaa aaaactgtag tatcctctgc
      901 gaaacgatcc ctgtttgagt attgaggagg cgagatgtcg cagacagaaa atgcagtgac
      961 ttcctcattg agtcaaaagc ggtttgtgcg cagaggtaag cctatgactg actctgagaa
     1021 acaaatggcc gctgttgcaa gaaaacgtct tacacacaaa gagataaaag tttttgtcaa
     1081 aaatcctctg aaagatctca tggttgagta ctgcgagaga gaggggataa cacaggctca
     1141 gttcgttgag aaaatcatca aagatgaact gcagagactg gatatactaa agtaaagact
     1201 ttactttgtg gcgtagcatg ctagattact gatcgtttaa ggaattttat ggctggccac
     1261 gccataaggt ggcagggaac tggttctgat gtggatgtac aggagccaga aaagcaaaaa
     1321 ccccgataat cttctttaac tttggcgagt cagaaagatt accggggccc acttaaaccg
     1381 tatagccaac aatcaagcta tgcggggagt atagttatat gcccggaaaa gttcaagact
     1441 tctttctgtg ctcgctcctt ctgcgcattg taagtgcagg atggtgtgac tgatcttcaa
     1501 caaacttatt accgccaggt aaagaacccg aatccggtgt tcacaccccg tgaaggtgcc
     1561 cggacgcttc cgttctgcgg gaaactgatg gaaaaggcgg tgggcttcac ctcccgtttt
     1621 gatttcgcca ttcatgtggc acatgcccgt tcgcttggtc tgcgtcggcg catgccaccg
     1681 gtactacgtc gccgggccat tgatgcgctg ctgcaggggc tgtgttttca ctatgaccca
     1741 ctggccaacc gcgtccagtg ttccatcacc acgctggcca ttgagtgcgg actggcgacg
     1801 gagtctgctg ccggaaaact ctccatcacc cgggccaccc gggccctgac gttccttgca
     1861 gagctgggac tgattaccta ccagacggaa tatgacccgc ttatcgggtg ctacattccg
     1921 accgatatca cgttcacatc tgcactgttt gctgccctcg atgtatcaga ggaggcagtg
     1981 gccgccgcgc gccgcagtcg tgtggaatgg gaaaacagac agcgcaaaaa gcaggggctg
     2041 gataccctgg gtatggatga actgatggcg aaagcctggc gttttgttcg tgagcgtttc
     2101 cgcagttacc agacagagct taagtcccgt ggaatgaagc gtgcccgtgc gcgtcgtgat
     2161 gcggacaggc agcgtcagga tattgtcacc ctggtgaaac ggcaactgac gcgtgaaatc
     2221 tcggaagggc gcttcactgc cagtcgtgag gcggtaaaac gtgaagtgga gcgtcgtgtg
     2281 aaggagcgca tgattctgtc acgtaaccgt aattacagcc ggctggccac agcttccccc
     2341 tgaaagtgac ctcctcagaa taatccggca cgcgccggag gcattcgcac gcctgaagcc
     2401 cgccagtgca caaaaaatca gcgctacata caaaaaacaa cctcatcatc caccttcagg
     2461 tgcatccggt cccccctgtt tttgatacaa aacacgcctc acagacgggg aattttgctt
     2521 atccacatta aactgcaagg gacttcacca taaggttaca accgttcacg tcataaagcg
     2581 ccagacgtca accctacagg gtgcaatgta tcttttaaac acctgtttat atctccttta
     2641 aactacttaa ttacattcat ttaaaaagaa aacctgttca ctgcctgtcc tgtggacaga
     2701 caaatatgca cctcccaccg caagcggcgg gcccccaacc gaagccggtt tagttacaac
     2761 atgcaaacat aaacccctaa aaaacccaag tccaacgcaa aactgaaacc acaacgcccc
     2821 cctcataact gaaaagcgcc gccgcccccg cccaaagggc gggaacagag tcgcttttaa
     2881 ttatgaatgt tgtaactaca tcatcatcgc tgtcagtctt ctggctggac gtatcgaata
     2941 cacgctcgta agcggccctc acggcccgct aacgcggaga tacgccccga cttcgggtaa
     3001 aaccctcgtc gggaccactc cgaccgcgca cagaagctct atcatggctg aaaacgggta
     3061 tggcttggca gggctgggga tgggtaaggt gaaatctgtc aatcagtacc ggctgacgcc
     3121 gggcttcggc ggttttactc cggtatcaca tgtaaccaca gcgtgccgcc ttccatgccg
     3181 ctggcgcggc atcagaataa tacaagcagc atttaatgca tttgcaaaag tgtgagtaat
     3241 attaagtggt cgtttcaggg atatcgttaa gacttgagcg ataggaatta aaaccccaaa
     3301 aagattaaaa aaacaccaca aaacggatgt ttcttcaaca ccacttttgc tccatatgaa
     3361 cggaaccgac gattaaactg gatggctctg attgattcag ggtatgaatg gcggtttttt
     3421 gctccgtttc cctcaaaatg gacgcaactt cccctctgcg gctctcagcc gcaccaccgc
     3481 atccgggcca gcggctcatg catcaggacc tgctctgcca gacggtagcc ccgcttcagc
     3541 cccgtaaaac gcatctgact ccc
//
LOCUS       DAETQL010000205         3548 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.209, whole genome shotgun sequence.
ACCESSION   DAETQL010000205 DAETQL010000000
VERSION     DAETQL010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.209"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="K0W65_004203"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="K0W65_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBI3097212.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="K0W65_004204"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="K0W65_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HBI3097213.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="K0W65_004205"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="K0W65_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBI3097214.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAETQL010000206         3465 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.210, whole genome shotgun sequence.
ACCESSION   DAETQL010000206 DAETQL010000000
VERSION     DAETQL010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.210"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..213)
                     /locus_tag="K0W65_004206"
                     /pseudo
     CDS             complement(1..213)
                     /locus_tag="K0W65_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            488..792
                     /locus_tag="K0W65_004207"
                     /pseudo
     CDS             488..792
                     /locus_tag="K0W65_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            961..2325
                     /locus_tag="K0W65_004208"
     CDS             961..2325
                     /locus_tag="K0W65_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3097215.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AASGLSGTFSYIFGSIVATLGMGLVVDYLGWGATFIVLILSAVFAIIFTLMSRERSLE
                     FEKE"
     gene            2375..2584
                     /locus_tag="K0W65_004209"
                     /pseudo
     CDS             2375..2584
                     /locus_tag="K0W65_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(2678..2869)
                     /locus_tag="K0W65_004210"
                     /pseudo
     CDS             complement(2678..2869)
                     /locus_tag="K0W65_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(2910..3407)
                     /locus_tag="K0W65_004211"
                     /pseudo
     CDS             complement(2910..3407)
                     /locus_tag="K0W65_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          846 a          782 c          823 g         1014 t
ORIGIN      
        1 tgaacctcga tcggtgtggt ggattagccc ggcaggtggg cgctggctcc tgagcgccat
       61 aaacagggct ttacccgtca actctttcgt catgcgctct cccatggcgt aaccgacaat
      121 ttcgcacgta taaacatctt tgacgccagc gagatacagc catccttccc gggtggcaac
      181 atacgttata tctgcgaccc agacctgatt aggggggttg gggagcaatg gaacagtaaa
      241 cgccgttaag aagtcaattt aaatagccaa aaattaacta gccaatttac attgcaggtt
      301 gtaattttta accacctttt ttacgatagc caaattacct atacccatcc cggccctgcc
      361 aagccatacc cgctatcggc gatgaaaatg cttttgtgcg cggtcggagt ggtcccgacg
      421 agggttttac ccgaagtcgg ggcgtatctc cgcgttagcg ggccgtgagg gccgcttacg
      481 agcgtgtatg agagacgtac agtggttttg tccgctcaca ccagtacata caggtacgga
      541 aataaaacca gtcgaaatgc cggtaacgac gattactcct gtctctgatg ccggtggact
      601 acaagatttt atttactggc gtcctgatgc ggacagggct ggtatggaac ctgtttatgt
      661 gatacttaat gatcctctgg attccgggcg atttacgcgt aaacaactaa ataaaaatat
      721 cttaaacatg caagtaattt tggtattctc gatacaagaa taaatagtag taactactat
      781 agctgcaaat aatgttaaaa acatttctat gatcatcttt acatttttca tgattgacaa
      841 ttgtttttgg ttaaaaaaag acaactaatg agtgtgataa tgcaatactt tgagtgatta
      901 tataatgata agtaaacagg tttgcgacaa ttggttatca gtattacaga aggattaaat
      961 atgtcattat tcacagaacc taaggaaatt gaaagattac cctctgaaga gattgaacga
     1021 ttatatcccg tcctgcgata cagggtcttt atcagcattt tcctcggata tatggggtat
     1081 tattttgtcc gaaacaccac cagtgtgtta tccggtgtgc tgcacatgtc tgcaaccgag
     1141 atcggcatta tctcctgtgc cggtttttta tcctatggta tatcaaagtt tgtttccgga
     1201 cttatttctg acagaagtaa ttcaaaagta tttttgtctc ttggtttatt cctgtccgga
     1261 ctggtaaatt ttctgatagg atatattccc gggatcatca catccgttac gttattttca
     1321 acaatgtatt tacttaacgg ctggatccaa ggtatgggat atcctcctgg tgccaaaaca
     1381 ctggtgttct ggtatgaaca tcgggaaagg atcacctggg ccacactgtg gaatttatcg
     1441 cacaatgtcg ggggagctct ggcccccgtt ctgattggtt tcagttttgg ttttttcggc
     1501 gacagtgctc tggatcatgc ccgggccgca tttatcttcc caggagtact gtgtatggca
     1561 atgagtgtgc tgatttactt tattcaggtg gacagacctg tatcagtggg gcttcctccc
     1621 attgaagaat ggaaaggtaa cgtcgtttca cacccggcta aaggccgtga acaaggtccg
     1681 agactctcca ttcctgatat catcagaaaa catatcatcc gaaataataa actcatttac
     1741 tgctgtatct atggttcgtt tgtttatatt ctgagatacg gtattgtttc ctgggctcct
     1801 aagtttttaa gtgacagtct ggatgtcggt ggtaaggata tgggaaaact ggcatcaatg
     1861 ggggggggtt ctgtttttga aatcggtggt gtggctggta tgcttcttgc cggatacctg
     1921 tctgtcagac tgttcagaaa ctcaaaacca ttaacaaata ctttatttct ggcacttacc
     1981 attattttat taatcgcgta ctggtacgtg ccatcaggca atgaatatct ctggctaaac
     2041 tatacaatcc ttattctgtt ggggctggca gtgtatggtc cggtgatgtt tattggtctt
     2101 tattcaatgg agcttgttcc taaagaagct gccggagccg catcagggct gtcaggcaca
     2161 ttttcctata tcttcggctc aattgttgcg acactgggaa tgggacttgt tgtcgattat
     2221 ctgggatggg gggccacatt tatcgtactg atactgtctg ctgtttttgc catcattttc
     2281 acactgatga gtcgtgaacg ctcactggag ttcgaaaaag agtaattttc tcaggaaaaa
     2341 cataaaatta ctgactaaat atttattttt tgaatcgcag ggcattccgg ataaggttga
     2401 tatcgtgtca cggctgaaga cctatggata cagtatttct ggtgtggaga cggatgacgg
     2461 atataaggcg cttgtccgcg cttttcagct tcatttccgg cagaaaaatt atgacggcat
     2521 aatggatgct gaaaccgctg ccatcctgta tgcattgctg gagaagtatt tcccgggtaa
     2581 ataaggagtg agtcgtcaga gctgcgatat atctccttcc tccatgggct gtctcttata
     2641 cacaaatccc cacccgggga tttgtgatgt cagatgtccc cagtaagact atgttttcat
     2701 tgcgctctat gaagctgagt gagcgtaacg actggagctg cttctgcggt gctccggtgg
     2761 cgaacgtgaa gtcatactct tcgaacattt tcaccgccgg gaaggctgcc attcgggtat
     2821 acatcgcctg tttacgttga tgacgtgcca gtttttcttc atgaagcatg atgctccaag
     2881 taaacgttca ccgaacaccg tattgacgat tatcctgatg gtagcaccac cttccatggc
     2941 aggaggtcgt caactcggtt ggaaggccat tccggcagta cgctcagaat atggcgcaga
     3001 tacgcttccg gatcgatacc gttcagacgg caggtgccga tcagcccgta cagcagtgca
     3061 ccacgctcgc cgccgtgatc gctaccgaag aacacgtaat ttttctttcc gagacagact
     3121 gcacgaagcg ctctttccgc tgtgttattg tccgcctccg ccagaccgtc atcactgtaa
     3181 taacagaggg cgtcccactg attcagtaca tagctgaatg cttcgcccag tctggatttt
     3241 ttcgacagcg tgccattctt ctccaccatc cattcatgca gcgacgtcag taacgctttg
     3301 cttcgctgct gcctgactgc aagacactct gactctggta atccccgtat ttcatcctcg
     3361 atggcgtaca gttcactgat tcgcttcagg gcttcttctg ccgtcgcacg caagtaatcc
     3421 ggggcccgct atgccgcgct ctatcgggcg ggacggggct ggcgc
//
LOCUS       DAETQL010000207         3414 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.211, whole genome shotgun sequence.
ACCESSION   DAETQL010000207 DAETQL010000000
VERSION     DAETQL010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.211"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..238
                     /locus_tag="K0W65_004212"
     CDS             <1..238
                     /locus_tag="K0W65_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HBI3097216.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="K0W65_004213"
     CDS             248..886
                     /locus_tag="K0W65_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBI3097217.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="K0W65_004214"
     CDS             889..2052
                     /locus_tag="K0W65_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBI3097218.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..>3414
                     /locus_tag="K0W65_004215"
     CDS             2136..>3414
                     /locus_tag="K0W65_004215"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBI3097219.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          760 a          803 c          987 g          864 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaact gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccagta acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgcaaacgag
     3301 gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat tgcgaggaga
     3361 gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa ggtt
//
LOCUS       DAETQL010000208         3348 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.212, whole genome shotgun sequence.
ACCESSION   DAETQL010000208 DAETQL010000000
VERSION     DAETQL010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.212"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            19..897
                     /locus_tag="K0W65_004216"
     CDS             19..897
                     /locus_tag="K0W65_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI3097220.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="K0W65_004217"
     CDS             894..2285
                     /locus_tag="K0W65_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBI3097221.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="K0W65_004218"
     CDS             2282..2539
                     /locus_tag="K0W65_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097222.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="K0W65_004219"
     CDS             2634..2834
                     /locus_tag="K0W65_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HBI3097223.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="K0W65_004220"
     CDS             2827..3207
                     /locus_tag="K0W65_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBI3097224.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          885 a          726 c          969 g          768 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaac
//
LOCUS       DAETQL010000209         3331 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.213, whole genome shotgun sequence.
ACCESSION   DAETQL010000209 DAETQL010000000
VERSION     DAETQL010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.213"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(153..419)
                     /gene="ospE2"
                     /locus_tag="K0W65_004221"
     CDS             complement(153..419)
                     /gene="ospE2"
                     /locus_tag="K0W65_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE2"
                     /protein_id="HBI3097225.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAANRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            complement(601..1002)
                     /locus_tag="K0W65_004222"
                     /pseudo
     CDS             complement(601..1002)
                     /locus_tag="K0W65_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1431..2219
                     /gene="virF"
                     /locus_tag="K0W65_004223"
     CDS             1431..2219
                     /gene="virF"
                     /locus_tag="K0W65_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HBI3097226.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(2212..2583)
                     /locus_tag="K0W65_004224"
                     /pseudo
     CDS             complement(2212..2583)
                     /locus_tag="K0W65_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            2582..3331
                     /locus_tag="K0W65_004225"
                     /pseudo
     CDS             2582..3331
                     /locus_tag="K0W65_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          979 a          640 c          688 g         1024 t
ORIGIN      
        1 taagttggca gcattaccgt atcgaggata gatggagtta ctcctctctg aaatagaatt
       61 actgctctac tttttctgtt tccagaaatt attagtaaca aaccctgata tattcttttg
      121 aaaatggagc ttaaacgagt ctgaaattaa cttcagaaat cagaataccg ttggctttgt
      181 ttttcatcaa ttccggcttc atttatcttt gtcaaccatt gcatttctgt agcagtgaat
      241 ttcaacgttt tcgtccggtt ggcagcaggt atttgtaatt cgtatatagc tgctttttta
      301 ttgaaaacag tatatccatc tgtagtaaca gtagaagata ataatacagg atttgatact
      361 tcaaggatga tattttcttg tttttgtgga aggcaaggga atatagtttg tgtaagcata
      421 ttgcttcctt atgttatatt ttagtaaaga atttattttt tacattatag tcggctaaat
      481 gaagtttact gtaaaaaaac gatacttttt tcgtaagcga tgagatgtgg agccagaaaa
      541 atggctgcgt tacgtcattg aactgtcctt gacccacgat attcagacag tcgttgtatc
      601 ttaagttgac attacccgct gacgtcgata ttcccttggt gagagataac ccaacgcact
      661 atgcggatgg ttttcattgt aatgctcgat cgccactgca aggttatgca atgccgttct
      721 tggtttcggc atgaacgcga tgcagtcttc cttcatcgtt ttcatgaacc gttctgctat
      781 tccattactc tgagggctac tgactgccgt cgtgcagggc tccagattca actctctggc
      841 gaactgccgt gtttcatgag ccgtataagc cgaaccgtta tcggccactg gattggccgt
      901 tccggtcctt tatcgccata tcgctcctga catgacatcc tgcaccgttg ctttgtcata
      961 atctcccgtg cctgctgccc agtctatggc ttcacgatcg caatatggaa cgtaacgctg
     1021 tcatcgggag aatcgtcaat gcggcattcg gttaacgctt accaatatca acaccaaccg
     1081 gatttgtgga gcctccagtc tgaaggggct ttatgcgttc cgtataggat attatgatgc
     1141 tggagttttg cgaagctcca ctcctttccg aacatttaca cagatattgc ctaagaaaag
     1201 tgaaaccata atatggttat agtccctttc agtgcaaata cttagcttgt tgcacagaga
     1261 aatagaagct gcataagctc tttcttcaaa aaatgtaaat aaagttaaat ataggaaaaa
     1321 ttacttaatc tatcttaata acggaaagat tttgtataca atcactgtta cagaaatttc
     1381 ttagttactc tgtaaacact aaatatagtt tggtatattc tgttgaattt atgatggata
     1441 tgggacataa aaacaaaata gatataaagg ttcgcttgca taactatatt attttatatg
     1501 caaaaaggtg ttcaatgacg gttagctcag gcaatgaaac tttgactatc gatgaagggc
     1561 aaattgcttt tatagagcga aatatacaaa taaacgtctc cataaaaaaa tctgatagca
     1621 ttaatccatt tgagattata agccttgaca gaaatttatt attaagcatt attagaataa
     1681 tggaaccaat ttattcattt caacactcct attctgagga gaaaaggggg ttaaacaaaa
     1741 aaatattcct cctctctgag gaggaggttt ctatcgattt gttcaaatct ataaaagaga
     1801 tgcctttcgg caaaagaaag atctatagtt tagcttgcct tttatcagct gtttctgatg
     1861 aggaagcttt atatacttcg atatcgatag cttcttctct tagtttttct gatcagataa
     1921 ggaagattgt tgaaaaaaac atcgagaaga gatggcgtct ttctgatatt tcaaataact
     1981 tgaatttatc agaaatagct gttagaaaac gattggagag tgaaaaatta acatttcaac
     2041 aaatccttct tgatattcgc atgcatcatg cagcaaagct tttattgaat agtcaaagct
     2101 atattaatga tgtatcaaga cttatcggaa tatcaagccc atcttatttt ataaggaaat
     2161 ttaatgaata ttatggtata actccaaaga aattttactt atatcataaa aaattttaaa
     2221 tgcttcatag cccatcgcta ttgccagatg ggttttcccc acacctgatg gccccagtaa
     2281 tacgatattt tcattacgtt ctatgaagct gagtgagcgt aacgactgga gttgcttctg
     2341 cggtgctccg gtggcgaatg tgaagtcata ctcttcgaac gttttcaccg ccgggaaggc
     2401 tgccattcgg gtatacatcg cctgtttacg ttgatgacgt gccagttttt cttcatgaag
     2461 cagatgctcc aggaagtcca tataactcca ttcctggtct actgcctgtt gtgacagcgc
     2521 aggcgctgcg cttatgtact gcacccattt tgttggacga tgaaatggaa tagcccctga
     2581 tatgtcaaag ccaaaatacc cttttgaaaa gcgccttgaa gtcgtgaatc actacttcac
     2641 tactgatgat ggttacagga tcatctcggc acgttttggt gtcccccgaa cccaggtcag
     2701 gacatgggtt gccctctatg aaaaacatgg agaaaaaggc ttaattccca aacctaaagg
     2761 tgttagtgct gatccagagt tgcgtattaa ggtcgtgaaa gctgtgattg agcagcacat
     2821 gtcccttaat caggctgctg ctcactttat gcttgctggt agtggttctg tagccaggtg
     2881 gctgaaggtt tatgaagagc gcggagaagc tggtttacgc gcgctcaaga ttggcaccaa
     2941 aagaaacatt gcaatatcag ttgatccaga aaaagcggca tcagcattgg agctgtcaaa
     3001 agaccgacgc attgaggatc ttgaaaggca agttcgattt cttgaaacgc ggcttgtgta
     3061 tctaaaaaag ctgaaagcct tagctcatcc cacgaaaaag tgaaagtact caacgagcta
     3121 aggcagtttt atcctcttga tgagcttctc agggctgcgg agataccgcg cagtacgttt
     3181 tattaccatc taaaggctct cagcaagcct gacaagtatg cggacgttaa aaagcgtatt
     3241 ggtgagattt atcatgagaa tagaggccga tacggatacc gtagggtaac gctgtctctt
     3301 catcgagaag ggaaacagat taaccataaa g
//
LOCUS       DAETQL010000210         3325 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.214, whole genome shotgun sequence.
ACCESSION   DAETQL010000210 DAETQL010000000
VERSION     DAETQL010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.214"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            454..1281
                     /locus_tag="K0W65_004226"
     CDS             454..1281
                     /locus_tag="K0W65_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBI3097227.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1325..2074
                     /locus_tag="K0W65_004227"
     CDS             1325..2074
                     /locus_tag="K0W65_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBI3097228.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2071..2640
                     /locus_tag="K0W65_004228"
     CDS             2071..2640
                     /locus_tag="K0W65_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097229.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2764..3006
                     /locus_tag="K0W65_004229"
     CDS             2764..3006
                     /locus_tag="K0W65_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBI3097230.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            3010..3156
                     /locus_tag="K0W65_004230"
     CDS             3010..3156
                     /locus_tag="K0W65_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097231.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            3165..>3325
                     /gene="xisR"
                     /locus_tag="K0W65_004231"
     CDS             3165..>3325
                     /gene="xisR"
                     /locus_tag="K0W65_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HBI3097232.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPKP"
BASE COUNT          928 a          796 c          855 g          746 t
ORIGIN      
        1 acgccatcct gacaagcgtt ttatcttcaa cgggaatgag atatggaaac gtggagaatc
       61 aaccacagag tatacccgcg cacaccagcg aacaatcaga cgactgcgca gactcatcgc
      121 caggaaacag tatacagaaa aatggttcag aaaatatgac acatggagcg ccggatatta
      181 cgaactgatg gcaacaaaac cattctgacg taaacgaaat taaccatgac gcaattaaat
      241 aaggcaagcc gaatacatca ggaggaccat gaacatttat ttcagaatag ttatatcact
      301 ggcaattatc gcatgtattt acggattaat cgttccggcc ctcatatcaa tgaaggatac
      361 ggtagcagtg atttctggct ttgctctggc gtgtctgacc ccgccctgca tttatgccat
      421 ttataagggt ctttcttttt ctaaggataa aagatgaaaa aagttattcc tgctttagcc
      481 cttgttctgc tgaccaccgg acttgtcggt tgcgatcgcg ttgagcctgg taatgttggc
      541 atcaaggtaa acaaactggg cgacgataaa ggcgtcggag aagtggtcgg tgttgggcgc
      601 tactggacgg gatggaacac tgaggtttac atcttcccga ccttcaaaca aatgaagacc
      661 tacgatgagc cattcagctt ccagatgagt gacggtacaa ccatcggcta tcacatcggc
      721 gtggcctaca aagttgatcc atccaaagtt accacagtct ttcagaccta ccgcaaaggc
      781 gtggacgaca ttaccgacac cgacctgcgc cagaagatag ccgacgcact caaccgactg
      841 gccagcaaaa tgaccaccga caaatttatc gacggcggca agtctgagct gctggatgca
      901 gctcttaaag acattcagga agaaatgacg cccatcggta ttcaggtaat gagcctctca
      961 tatgtgggta agccggagta cccgcctact gttatcgaca gcattaatgc caaagtcacg
     1021 gcgaaccaga aaaccctgca atgcgagcag gaagtaaaac agcgcgaagc ggaagccaac
     1081 atgttgcgcg cggaagctgc cggacaggca gatgcgattc gcacaaaagc ccaggccgaa
     1141 gccgatgcta ttcgtttacg cggtgaagct ctgcgccaga acccaaacgt tatggagctg
     1201 gaagccatca acaagtggaa cggtacactg ccgcaataca tgaccagcgg tgccaacaca
     1261 ccatttattc aggttaagta atacatatgc ccggtattac acgccggggt gtctggagat
     1321 aaaaatgaat actgtaacca tcaacaacaa acagtttccg gtaatcgaat atcgcggtca
     1381 gcgtgttgtg acgctggcga tgattgatga agtccaccag cgaccggaag gaaccgcgcg
     1441 tgcagcattc aaccgaaacc gtgagcattt catcagcggt gtggattatg ccgaattagg
     1501 tgcggacgta atacgtacgg acctcccgga aggaacattc tctaaatttg caccatcggg
     1561 gattgtactt tttgaatcag gttacctgat gttgacgaag ccattcaatg acgcccttgc
     1621 ctggcaggtt cagcgcgaac tggttaacag ctacttccgc actcgcacac cgctaacgga
     1681 aatcgagatg atcgctgcga tagccgccga cgctgttcgt caacagaagc gcctgaatca
     1741 tgtggaagaa cagctcgaaa cagtcacaga agcggtggaa accatcaaac gcggaaatat
     1801 gcgcgccgga tatgtcggtt accgccaggt ggtagccaaa agtggaatga gtgacaccaa
     1861 gtgtcggaat ctggtgaacg catatcaaat tcctacagac acccacgaat tcatgactcc
     1921 ggacggtctg ttgtctcgca gggctatcgt cgagcttgag ccatttatgg cggcgttcca
     1981 ccaaatgatg tcagaggctg acccacgcgg cacacgctgg tatcacccga agatggggct
     2041 tttccaggtg atcggatggg aggataaagc atgatcatcc agtcaaaact tattcgcgcc
     2101 gctctggtgt gcgctgctaa aaacgacgtt cgttattacc tgaacggtct tcacatcacg
     2161 ccgaaacata ttgaggcaac caatggttcc gtggcactgc gcatggctca cggcatccgg
     2221 acgaagaaaa acatcattgt ccagttcgaa gggggcgtcc cggccaaagc cgaaacgaca
     2281 gagctgattt ttagtaaaga gccgatcgct gttcatcgcg accagtttca gcgacgactg
     2341 tccattaccg gcattaaact ggtggacggt tgttttccgg atttggatcg catcattccg
     2401 aaaaagtttg accgctgtac tcacccggtg ctccaggcgg gttacctgag ttatccagag
     2461 aagatgtttg gtcgtgagcg taaatttatt cccgtccagt tacgtccctc cggtgacggg
     2521 caagcggtca gaattcagtt tgattccatc atcaactcaa tgtatggcaa tcctgaattt
     2581 gttgtgatgc cttgtcgtga tcatggcgat ttcaacgtgg ctcaggagca tccggaatga
     2641 aaatcgaata ccaggacgcc gcaggaggtg aatcaatgag ctggcctgat gcaatcgtaa
     2701 ctctggggat ggtattcgca gtagcgtttg ttgtgtactc gatttgtcga tggggataac
     2761 cacatgttcg ctttgattca acgcgggcag atatacacag acagagccgg atacccagta
     2821 gaaattactc gcagtactga gcactcagtg ttctttcgga ggatggacgg acgtaccggg
     2881 cgagtacgca tcggtgaatt cagtagcctg ttcgagcata ttgaccacct ggaatatcac
     2941 aagattctgg ctgaaacaga acaagaaaag cacctgaaga aattacgcgc aatgcaaagg
     3001 aagtaaaaaa tgaataaagc gtttgagcta tgggtccacc agcgttacgg caaccactac
     3061 gacctgacgc gagatgttga cggtttctac tgtcgtgaaa ttgtgaaacg aatgtttgag
     3121 gtctggtgcc actgccgtgg gctgagtgtt gtgtgaggta atgcatgggc aatgtgattc
     3181 aactggctcc caatgaatgg gtttgtgaaa gcgttctaat cgcaattacc gggctcaaac
     3241 caggcacaat tcttcggacc cggaaagaat gctggatggt tggaagagag tatattcacg
     3301 tatcaccaga cggtaatcca aagcc
//
LOCUS       DAETQL010000211         3325 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.215, whole genome shotgun sequence.
ACCESSION   DAETQL010000211 DAETQL010000000
VERSION     DAETQL010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.215"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..109
                     /locus_tag="K0W65_004232"
     CDS             <1..109
                     /locus_tag="K0W65_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBI3097233.1"
                     /translation="RYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            167..523
                     /locus_tag="K0W65_004233"
     CDS             167..523
                     /locus_tag="K0W65_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097234.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            616..834
                     /locus_tag="K0W65_004234"
     CDS             616..834
                     /locus_tag="K0W65_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HBI3097235.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            836..1201
                     /locus_tag="K0W65_004235"
     CDS             836..1201
                     /locus_tag="K0W65_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBI3097236.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            1198..1863
                     /locus_tag="K0W65_004236"
     CDS             1198..1863
                     /locus_tag="K0W65_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097237.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1863..2228
                     /locus_tag="K0W65_004237"
     CDS             1863..2228
                     /locus_tag="K0W65_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HBI3097238.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2683..2838
                     /gene="hokD"
                     /locus_tag="K0W65_004238"
     CDS             2683..2838
                     /gene="hokD"
                     /locus_tag="K0W65_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBI3097239.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          963 a          678 c          857 g          827 t
ORIGIN      
        1 ccgctacgac aggcgcctga atataatctg tcgcgagtgc cggaagagcg aagctatgca
       61 gcgtgtactg gctttctatc agggtaattt tcaggaggcg atactgtgaa tgaaattagc
      121 tatcaggctt caattaccgc tggcattcgc atcaaaggag aggagcatgg aaataaaacc
      181 agaagatgag ttaagcaata tcgttttatt tccgataaaa gaggatgacc ctcgtaatca
      241 ggttaatttt ctttataagc catcggaaag accatattgc catcacgcct ctgtacgggt
      301 tgacgaaaaa gagcgtcagg tccgctgtaa aatctgcggt gcggttgtgg agccgtttga
      361 ctggatgctc tctgtggcaa aaagagaaac cagactggca gatgatgtaa ggctattgcg
      421 ccaggaggaa caggaaaggc ggagaaatat agaaaagctg atacagattg agcgtaacgc
      481 gaaagcgcgg atacgcaggg cgacaaaatc cagaactgaa taattaaatt tagcactgtt
      541 aaaaatttaa tccttaaccg gagggatttc tgcaccctca aatcatcagg agaccgcccg
      601 aaagggcggt aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca
      661 acagaattcc tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt
      721 tttgtctgga taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta
      781 tgggcgtcat tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc
      841 actgacgaaa aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg
      901 cggctgcgaa cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga
      961 tggaaatatg cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc
     1021 atgcaatctg cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg
     1081 tcgtcgtgaa ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga
     1141 aatgaaatct gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg
     1201 actactatta ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt
     1261 gacgatatag acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa
     1321 gcagagcctg tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt
     1381 gagtatgtgg gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg
     1441 cgacaacctg atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt
     1501 gaccaggaat ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg
     1561 gtacgactgc ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg
     1621 gcagtgccgg ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat
     1681 tcatcgcttg gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc
     1741 aacgtcacta tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat
     1801 acgtttgatt atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa
     1861 taatgattaa tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta
     1921 ttgggcaact ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc
     1981 gtaaggctgg caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc
     2041 ctgctgaata ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct
     2101 gggataaggt tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg
     2161 gcgttgaagt agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga
     2221 atcaatgacc tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt
     2281 ggtgtattcg atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt
     2341 ttttgaagga aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta
     2401 aacgtatctc aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa
     2461 ggtgctggca ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct
     2521 tgaggctgtc tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca
     2581 ttacgcgtcc tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt
     2641 aggttagcct cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca gcaaaaggcg
     2701 atgttaatcg ccctgatcgt catctgttta accgtcatag tgacggcact ggtaacgagg
     2761 aaagacctct gcgaggtacg aatccgaacc ggccagacgg aggtcgctgt cttcacagct
     2821 tacgaacctg aggagtaaga gaccaggcgg gggagaaatc cctcgccacc tctcatgtgt
     2881 caggcatcct caacgcaccc gcactaaacc cgcttcggcg ggttttttgt tgcgtgctga
     2941 atacgcaggg tgaaaaataa ccatatattt gattatatac acaacaaaaa ataaaagtca
     3001 ttgtacttgc acattaaaca atcaaatata cggcgtgaaa taaatattta tcagattaat
     3061 atttttgtct ctatgtggat ataaccgttt gtacttataa acctgggggc atcgtggaaa
     3121 aaataaagaa actatttagt agcaaatacg cagtcatacg tcgtgatgac ctgtcagtta
     3181 tagtcgaaat ggattacttc cctgaaaccc caaaatcaat aatgtatcgt aatggtcgaa
     3241 aggcaatttt tttaccgatg agggtaagtg acattatggg aaatgataaa ctgctggatg
     3301 aattgcgagt cagagcatcc tgtta
//
LOCUS       DAETQL010000212         3204 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.216, whole genome shotgun sequence.
ACCESSION   DAETQL010000212 DAETQL010000000
VERSION     DAETQL010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.216"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..885
                     /locus_tag="K0W65_004239"
     CDS             <1..885
                     /locus_tag="K0W65_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBI3097240.1"
                     /translation="QLRDITESKIYSAMQKMTNRRHEENWRLRAEACRKKGKPVPEYT
                     PKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLI
                     DECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRRVAWINPEESKSNRAIGVALND
                     TACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDF
                     RFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILN
                     PSVPNSSQSKNKEGTNDV"
     gene            1234..2406
                     /locus_tag="K0W65_004240"
     CDS             1234..2406
                     /locus_tag="K0W65_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBI3097241.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2460..3083)
                     /locus_tag="K0W65_004241"
     CDS             complement(2460..3083)
                     /locus_tag="K0W65_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3097242.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
BASE COUNT         1005 a          668 c          639 g          892 t
ORIGIN      
        1 caactaagag acattactga atcaaaaatt tattcagcaa tgcagaaaat gacgaaccgg
       61 cgtcatgagg aaaactggag actcagggca gaagcatgca gaaaaaaagg gaaacctgtt
      121 ccagaataca cgccaaaacc agcgtccgtt gcaacgaagg ctacgcatct ttcatttata
      181 aaggccctac taagagccgc agagcgtgaa tggaaaatgc tggataaggc accaattatt
      241 aaagtgcctc aaccaaagaa taaacggatc cgctggctgg agccccatga agcacaaagg
      301 ctgattgatg aatgtccgga gccattaaag tctgttgttg aatttgcact ggcaacaggc
      361 ttaagacgct cgaacatcat caaccttgaa tggcaacaaa tagatatgca gcgccgggtg
      421 gcatggataa acccggaaga gagtaaatca aaccgcgcaa ttggcgttgc gctgaatgat
      481 actgcatgtc gcgtattgaa aaaacaaatc gggaatcatc accgttgggt atttgtgtac
      541 aaggaaagct gtaccaaacc agacggaacg aaagcgccaa cggtcaggaa gatgcggtat
      601 gacgcaaaca cagcctggaa agcggcgctg agacgggctg gtattgatga tttcagattt
      661 cacgacttga gacacacctg ggcaagttgg ctggttcaag ccggagtccc gttatcagtg
      721 ttacaggaaa tgggaggctg ggagtctatc gaaatggttc gtcgatatgc tcaccttgca
      781 cctaatcacc ttaccgaaca cgcacggcaa atagactcga tcctgaaccc atcggtccca
      841 aattcgtccc agtcaaaaaa taaggaaggt actaatgatg tgtaacttat tgatttaaat
      901 ggtgccgata ataggagtcg aacctacgac cttcgcatta cgaatctgta gcaccaatca
      961 taactatctg ttttagcaag cattaaccgc attcactaag ctatagttga tgacacaaac
     1021 agaaagttga tgcatgatgt tgccatatat atgtcacaaa tacggcacaa cgatcttcaa
     1081 acatatagcc acacattcac agaagagcac aaagccttgc aatccagtgc aaagcttggt
     1141 gtgtctccgt tttgtttcat cagttacaca taactatatg atcattaact aaaagtcagt
     1201 atacttggag aaattttaat tgcgtaatag agcatgcata tgattgaaat taacagttta
     1261 ttattaataa catccgtgat attgatgtcg ttattagctg tggggttatt tgataaaatt
     1321 tccccaataa atcttgttga gcacggcagg aataatcaaa tagatggaat gcgggggttc
     1381 ttagcaattt tcgtgcttat tcatcacgca gcaatttgga atggatactt gtcatctgga
     1441 gtatgggaag caccttcatc aaatctgtta gcaaatttag gccaagttgg tgtgtcattc
     1501 ttttttatga ttactggtta tctgttcttt tcaaagatta tctctggaga tcaggactgg
     1561 acaaggcttt atgtatcaag attactacga ttaaccccaa tgttcatagt tagcctatgt
     1621 ctcattttta tcattgtagg ttttaagtct ggatggagaa tgcaggtatc cacagaagag
     1681 ctttttgtgt caataatgaa gtggttgcca ttcactgcgc taggtatgcc gaacattaat
     1741 gacgtaaaag attcatttac tatcaatgcc gctgtaacat ggacacttgt atatgaatgg
     1801 ttcttttatt tttctcttcc ggtaatttcc gcgctcataa aaagaaaagt cagtatttac
     1861 atggttatga ttagcgcaat atcgctattt gttttcattt tatttttcag taaaatacac
     1921 atagcatcat ttctttttgg gctcctagca tttttgctaa ataaatcaaa gatagtcaat
     1981 ggaattgcca aagcaaaagt aacaccaatt ataataactg cgataatggt ttttgaaatg
     2041 acgtatttca aaacaactta cgcaccgcta ccactgattc tttgtggaat aacatttatc
     2101 attatcgcat caggttgtga tttgtatgga atattaagat taaatataac cagaaagcta
     2161 ggagaaacta cctacagcgt ttaccttctg catgggatat tcctttattg cctaatgacg
     2221 tggattattc ctaataatta cactgaaaat acatttatca tactggtaag cacaactgca
     2281 tttcttatta cgtttacatc atgcctaact tttaaattaa tagagacgcc attcatcaaa
     2341 ttaacaaaac aaaccacaac tttagtaaaa gaattaatac ccacattaac aaacaacaat
     2401 caataaacaa taaacataag ccgccctaca tatatgtcca aatggggcgg cgaatgtatt
     2461 tatattagtc tggtaatgct aaatgcaaac gaactggaga ttcctgcccc gctaagcgta
     2521 ttgcgcaggg tcaccctaaa tgtatctacg ccaatataag taacatcaca actaactgaa
     2581 tcaggcgcag atataggtgt tatctgcaca atataaccgc tagcaactgc tgcgagcgga
     2641 tgagaaaagc taacatcata cacacctgta gaaaccacgt taaccgatga aacaccagta
     2701 gcaacgtcga tactaccatt ggcgttaatt tttcccgagc gtgtgactga caatgcagaa
     2761 gccgatgtta ccttactggt tgtttctgta aaaacacacc caatgtcaac tacctcgcaa
     2821 ttagctcctg ttatccagta tggtcgttct gcacttggct gataatcccc tgcgctctgg
     2881 aacgttgtgc cgttgagtat gactgtaact tttcccccac ctgagcttgt tacttgaata
     2941 ttcgtgtgcg tgtatcttgc actgctcacg cgatgaaaat ttccaccgtt tataaccaca
     3001 gttacagggc gcgtcccagt attatctata gcaaggtctg cccccccggc attagcctca
     3061 aagtatgggt tgtttaatac caaagcgcac gaaccgttaa ggccatttat agctaattgc
     3121 gcaccacctg tactggaatc cccctgagta ccatttcctt cacaagttga ggtgtactgg
     3181 caatagcgga cactaccatt tgtt
//
LOCUS       DAETQL010000213         3189 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.217, whole genome shotgun sequence.
ACCESSION   DAETQL010000213 DAETQL010000000
VERSION     DAETQL010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.217"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            169..1272
                     /gene="proB"
                     /locus_tag="K0W65_004242"
     CDS             169..1272
                     /gene="proB"
                     /locus_tag="K0W65_004242"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBI3097243.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1284..2537
                     /gene="proA"
                     /locus_tag="K0W65_004243"
     CDS             1284..2537
                     /gene="proA"
                     /locus_tag="K0W65_004243"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBI3097244.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            2652..2727
                     /locus_tag="K0W65_004244"
     tRNA            2652..2727
                     /locus_tag="K0W65_004244"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2685..2687,aa:Thr,seq:cgt)
     gene            complement(2742..>3189)
                     /locus_tag="K0W65_004245"
     CDS             complement(2742..>3189)
                     /locus_tag="K0W65_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI3097245.1"
                     /translation="ESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGT
                     KAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWE
                     SIEMVRRYAHLAPNHLTEHARQIDLILNPSVPNLSQSRNKEGTNDV"
BASE COUNT          752 a          807 c          878 g          752 t
ORIGIN      
        1 gtggtaattt attaaatctg taataaaagc gtaaacaact gccgctacgc ttgctgatcc
       61 cgcgcaacaa aacgccatgc tttgctcgca gatggttggc aaccgacgac agtcctgcta
      121 aaacgttcgt ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc
      181 cagacgctgg tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgtctgaac
      241 cgtgcccata tcgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg
      301 attgttattg tgacgtcggg cgcgatcgcc gccggacgtg agcacctggg ttacccggaa
      361 ctgccagcga ctatcgcctc gaaacaactg ctggcggcgg tagggcagag tcgactgatt
      421 caactgtggg aacagctgtt ttcgatttat ggcattcacg tcgggcaaat gctgctgacc
      481 cgtgctgata tggaagaccg tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg
      541 ctcgataaca atatcgttcc ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt
      601 aaggtcggcg ataacgataa cctttctgcg ctggcggcga ttctggcggg tgccgataaa
      661 ctgttgctgc tgaccgatca aaaaggtttg tataccgctg atccgcgcag aaatccgcag
      721 gcagaactga ttaaagatgt ttacggcatt gatgacgcac tgcgcgcgat tgccggtgac
      781 agcgtttcag gcctcggaac tggcggcatg agtactaaat tgcaggccgc tgacgtggct
      841 tgccgtgcgg gtatcgacac cattattgcc gcgggcagca agccgggcgt tattggtgat
      901 gtgatggaag gcatttccgt cggtacgctg ttccatgccc aggcgactcc gcttgaaaac
      961 cgtaaacgct ggattttcgg tgcgccgccg gcgggtgaaa tcacggtaga tgaaggggca
     1021 actgccgcca ttctggaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact
     1081 ggcaatttct cgcgtggtga agtcatccgc atttgcaacc tcgaaggtcg cgatatcgcc
     1141 cacggtgtca gtcgttacaa cagcgatgca ttacgccgta ttgccggaca ccactcgcaa
     1201 gaaattgatg caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg
     1261 attacccgtt aaggagctgg ccgatgctgg aacaaatggg cattgccgcg aagcaagcct
     1321 cgtataaatt agcgcaactc tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg
     1381 atgaactgga agcacaaagc gaaatcatcc tcaacgctaa cgcccaggat gttgctgacg
     1441 cgcgagccaa tggccttagc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc
     1501 tgaaaggcat tgccgacgat gtacgtcagg tgtgcaacct cgccgatccg gtggggcagg
     1561 taatcgatgg cggcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg
     1621 gggttattgg cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt
     1681 gcctgaaaac cggtaatgcg gtgatcctgc gtggtggtaa agaaacctgt cgcactaacg
     1741 cggcaacggt ggcggtgatt caggacgccc tgaaatcctg tggcttaccg gcgggtgccg
     1801 tgcaggcgat tgataatcct gaccgtgcgc tggtcagtga aatgctgcgt atggataaat
     1861 acatcgacat gctgatcccg cgtggtggcg ctggtttgca taaactgtgc cgtgaacagt
     1921 cgacaatccc ggtgatcaca ggtggtatag gcgtatgcca tatttacgtt gatgaaagtg
     1981 cagagatcgc tgaagcatta aaagtgatcg tcaacgcgaa aactcagcgt ccgagcacat
     2041 gtaatacggt tgaaacgttg ctggtgaata aaaacatcgc cgatagcttc ctgcccgcat
     2101 taagcaaaca aatggcggaa agcggcgtga cattacacgc agatgcagct gcgctgacac
     2161 agttgcaggc aggccctgcg aaggtggtgg cggttaaagc cgaagagtat gacgatgagt
     2221 ttctgtcatt agatttgaac gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata
     2281 ttcgtgaaca cggcacacaa cactccgatg cgatcctgac ccgcgatatg cgcaacgccc
     2341 agcgttttgt taacgaagtg gattcgtccg ctgtttacgt taacgcctct acgcgtttta
     2401 ccgacggcgg ccagtttggt ctgggtgcgg aagtggcggt aagcacacaa aaactccacg
     2461 cgcgtggccc aatggggctg gaagcactga ccacttacaa gtggatcggc attggtgatt
     2521 acaccattcg tgcgtaaata aaaccgggtg atgcaaaagt agccatttga ttcacaaggc
     2581 cattgacgca tcgcccggtt agttttaacc ttgtccaccg tgattcacgt tcgtgaacat
     2641 gtcctttcag ggccgatata gctcagttgg tagagcagcg cattcgtaat gcgaaggtcg
     2701 taggttcgac tcctattatc ggcaccattt aaatcaataa gttacacatc attagtacct
     2761 tccttatttc ttgactggga caaatttggg accgatgggt tcagaatcaa gtctatttgc
     2821 cgtgcgtgtt cggtaaggtg attaggtgcg aggtgagcat atcgacgaac catttcgata
     2881 gactcccagc ctcccatttc ctgtaacact gataacggga ctccggcttg aaccagccaa
     2941 cttgcccagg tgtgtctcaa gtcgtgaaat ctgaaatcat caataccagc ccgtctcagc
     3001 gccgctttcc aggctgtgtt tgcgtcatac cgcatcttcc ttactgttgg cgctttcgtt
     3061 ccgtctggtt tggtacagct ttccttgtac acaaataccc aacggtgatg atttccgatt
     3121 tgttttttca atacgcgaca tgcagtatca ttcagcgcaa cgccaattgc gcggtttgat
     3181 ttactctct
//
LOCUS       DAETQL010000214         3179 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.218, whole genome shotgun sequence.
ACCESSION   DAETQL010000214 DAETQL010000000
VERSION     DAETQL010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.218"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="K0W65_004246"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="K0W65_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBI3097246.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLIGVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVGFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="K0W65_004247"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="K0W65_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          799 a          659 c          776 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattggc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg ggttttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAETQL010000215         3136 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.219, whole genome shotgun sequence.
ACCESSION   DAETQL010000215 DAETQL010000000
VERSION     DAETQL010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.219"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..199
                     /locus_tag="K0W65_004248"
     CDS             <1..199
                     /locus_tag="K0W65_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBI3097247.1"
                     /translation="KLVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIK
                     QRGVNVFIQDVPGDQKEQIPD"
     gene            238..1041
                     /gene="agaC"
                     /locus_tag="K0W65_004249"
     CDS             238..1041
                     /gene="agaC"
                     /locus_tag="K0W65_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HBI3097248.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1031..1822
                     /gene="agaD"
                     /locus_tag="K0W65_004250"
     CDS             1031..1822
                     /gene="agaD"
                     /locus_tag="K0W65_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HBI3097249.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASLGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1823..2578
                     /locus_tag="K0W65_004251"
     CDS             1823..2578
                     /locus_tag="K0W65_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HBI3097250.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            2979..3071
                     /locus_tag="K0W65_004252"
                     /pseudo
     CDS             2979..3071
                     /locus_tag="K0W65_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
BASE COUNT          814 a          697 c          742 g          883 t
ORIGIN      
        1 taaattggta gaaggtggca ttgacctgaa agatgtcaac gtcggcaata tgcatttctc
       61 ggaagggaaa aagcaaatca gcagtaaagt ttatgtcgat gaccaggatc tcgcggactt
      121 acgttttatt aaacaacgtg gcgtgaatgt tttcattcag gacgtccctg gcgatcaaaa
      181 agaacaaatc cctgactaaa tctaaaatcg ccttaatatt ggtttgaggt aataaaaatg
      241 catgaaataa ccctacttca gggattatcc ctagcggcgt tagttttcgt tctggggatt
      301 gatttttggc tggaagcctt atttttattc cgcccgataa tcgtttgtac cctaactggc
      361 gctattctcg gtgatattca gactggctta attaccggtg gtctgacaga gttggctttc
      421 gccggattaa cccctgcagg tggtgttcag ccgcccaacc cgattatggc gggtctgatg
      481 accaccgtca ttgcatggtc tacgggcgtt gatgccaaaa cagcaattgg tcttggcctg
      541 ccgtttagtt tgttaatgca gtacgtcatt ctgttcttct attccgcttt ctcattattt
      601 atgaccaaag ccgataaatg cgcgaaagag gcggatacgg cagcgttttc ccggcttaac
      661 tggacaacga tgctcatcgt cgcttcagcg tatgcggtga ttgctttcct ctgtacttac
      721 ctggcacaag gggcgatgca ggcgctggtg aaagcgatgc ccgcctggtt gacccacggc
      781 tttgaagtgg ctggcggtat tctgcctgcc gttggttttg gcttgctgct gcgcgtaatg
      841 ttcaaagcgc aatatatccc ttacctgatc gccggtttcc tgtttgtttg ctacatccgg
      901 gtcagcaacc tgttgccggt tgccgtactg ggcgcaggct ttgcggtgta tgagtttttc
      961 aatgcgaaat cccggcagca agcgcaaccg cagcccgttg ccagtaaaaa tgaagaagag
     1021 gactacagca atgggatctg aaatcagtaa aaaagatatc acccgtctgg gctttcgttc
     1081 gtcgctgctg caagcgagct ttaactacga aaggatgcag gcgggcggtt ttacctgggc
     1141 gatgttgccg atcctgaaaa agatttataa ggacgacaaa ccgggcttaa gcgcggcgat
     1201 gaaagataac ctcgaattta ttaataccca cccgaatctg gtcggattcc tgatggggtt
     1261 attaatttcg atggaagaaa aaggagaaaa ccgcgacacc attaaaggcc tcaaagtggc
     1321 actgtttggc ccaatcgccg ggattggcga tgcgattttc tggtttactt tgttgccgat
     1381 tatggcggga atttgctcat catttgccag cctgggaaac ctgctggggc cgattctatt
     1441 tttcgccgtt tacctgctta tctttttcct gcgcgtcggc tggacccacg tcggttattc
     1501 agtcggcgtg aaggcgatcg ataaagtgcg agagaactcg cagatgattg ctcgctcggc
     1561 aaccatcctc gggatcacgg taatcggcgg gctgatcgct tcgtatgtgc atattaacgt
     1621 ggtgacatcg tttgccatcg acagtaccca cagcgtcgcg ctgcaacagg atttcttcga
     1681 taaagtcttc ccgaacattt taccgatggc ctacaccctg ctgatgtatt acttcctgcg
     1741 ggtgaaaaaa gcgcatccgg tgctgttaat cggcgtgact tttgtgctct ctattgtttg
     1801 ttccgcattc ggcattttgt aaatggaacg aggcactgct tctggcggtg cctctttact
     1861 taaggaattt catcctgtgc aaacccttca gcaagttgaa aactatacgg cgttaagtga
     1921 acgagccagc gaatatttat tggctgtgat ccgtagcaaa ccggatgccg tgatttgcct
     1981 ggcgaccgga gccacgccat tactgacgta tcattatctg gtagaaaaaa tccaccagca
     2041 gcaggttgat atcagccagc tcaccttcgt gaagctcgac gaatgggtgg atctgccatt
     2101 aacgatgcct ggcacctgcg aaactttcct acaacagcat atcgtgcagc cgctggggct
     2161 acgtgaagac cagctcatca gctttcgctc cgaagagata aatgagacag agtgcgaacg
     2221 ggtaacgaac ctgattgcgc gcaaaggcgg tctggattta tgcgttctcg gattggggaa
     2281 aaacggtcat cttgggctga acgaaccggg agaaagccta caaccggcct gccatatcag
     2341 tcaacttgat gccagaacac agcaacatga gatgttaaaa accacgggtc gccccgtgac
     2401 tcgtgggatc accttaggcc tgaaggatat tctcaatgcc cgcgaggttt tgttattggt
     2461 gactggcgaa ggaaagcagg atgcgacaga acgttttctc acggctaaag tctccaccgc
     2521 tatcccggct tcatttttat ggctgcacag taactttatt tgtttaatta atacctaacc
     2581 agatgaaaaa tttgctcaaa atttttaaat taacttattc aacagtcacg cattatatta
     2641 aataacattt gactgggttg aacataacgc caatagcaaa aggagttatg ttcaacaatt
     2701 taattttaga tatacatata acttgttcta ttaataaatc acctcgtatt ctgactcggc
     2761 ccctggcgca gcaaagtcag gcatttatac tctttttaac acatagaaaa ttcatatcaa
     2821 tataataaaa acataaaata tttgcatata catataatct taaagtttag tctatttaat
     2881 gttcaatgaa atatttctac ctgtataatc tttaaagatg ttgaacatat attcacatta
     2941 aatatgatta tgtacttgtt acaaggataa ggttataaat gaataaagtt acaaaaacag
     3001 ctattgtggg cttacttgcg cttttcgcag gtaatgccgc tgcaaccgat ggtgaaatag
     3061 tttttgatgg cggtaatgac tccaacttac tgatagtgtt ttatgttcag ataatgcccg
     3121 atgaccttgt catgca
//
LOCUS       DAETQL010000216         3115 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.220, whole genome shotgun sequence.
ACCESSION   DAETQL010000216 DAETQL010000000
VERSION     DAETQL010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.220"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="K0W65_004253"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="K0W65_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HBI3097251.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="K0W65_004254"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="K0W65_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="K0W65_004255"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="K0W65_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HBI3097252.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="K0W65_004256"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="K0W65_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HBI3097253.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3115)
                     /locus_tag="K0W65_004257"
                     /pseudo
     CDS             complement(2163..3115)
                     /locus_tag="K0W65_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          775 a          754 c          758 g          828 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc cagctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gggca
//
LOCUS       DAETQL010000217         3069 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.221, whole genome shotgun sequence.
ACCESSION   DAETQL010000217 DAETQL010000000
VERSION     DAETQL010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.221"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..498
                     /locus_tag="K0W65_004258"
     CDS             <1..498
                     /locus_tag="K0W65_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBI3097254.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            578..1273
                     /locus_tag="K0W65_004259"
     CDS             578..1273
                     /locus_tag="K0W65_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBI3097255.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1302..>3069
                     /locus_tag="K0W65_004260"
     CDS             1302..>3069
                     /locus_tag="K0W65_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI3097256.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPSTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          889 a          718 c          683 g          779 t
ORIGIN      
        1 gatggcgaga ttttaaaatc cgcttgtgaa atcaatgact ctgataagaa aattgaagtt
       61 gctcttggtc actataatgc cgaacagttt cgttctgttg gcgatcgtag cccaaaaatt
      121 ccattcacta ttcctttagt taactgccca gtgactgggt gggagcacga taacggcaac
      181 gttgaagcgt ctttccgttt atggctggaa acgcgggata atggcaccgt ccctaacttc
      241 cctaatctgg caaaagtggg ctcttttgct ggcacagcga caacaggtgt gggtatccgt
      301 attgacgatg cggaaagcgg aaaccttatg ccactgaatg ctatggggaa tgataacacg
      361 atttatcaga tcccagcgga ctccaatggt attgtcaatg ttgacctcat cgcttactac
      421 gtatcaaccg ttgaagcatc agaaatcacc ccaggggaag cagacgctgt cgttaacgta
      481 acgctggatt accgttaata cgttacggcg ttatctgacc tttcagataa cgcccttttc
      541 cttcctcttt ctcgttgtat caggttgaaa aataactatg tcaaaacgaa cattcgcggt
      601 gatattaacc ttgttgtgta gcttctgtat cggccaggcg cttgcagggg gtatcgtttt
      661 acagcgaacg cgagtgatct atgatgccag ccgcaaagag gctgcgttac ctgtcgcaaa
      721 caaaggcgca gaaacgcctt atttactgca atcatgggta gataatatag atggtaaaag
      781 ccgtgcccca tttattataa ccccaccgct atttcgtctt gaggctagcg atgactcatc
      841 actgcgaatt attaaaacag ctgataacct gcctgaaaat aaagagtcgc tgttctacat
      901 taatgttcgt gccattccag caaagaaaaa atcagatgat gttaatgcta acgagttgac
      961 gctggtattt aaaacgcggg tcaaaatgtt ttatcgcccc gcacacctga agggacgggt
     1021 aaacgatgcg tggaaatcac tggaatttaa acgtagtgac cattcactca atatatataa
     1081 cccaactgaa tattacgtcg tatttgccgg actggcagtc gataaaaccg atctcacaag
     1141 caaaattgaa tatatcgcgc ccggagaaca taaacagtta ccacttccag catctggcgg
     1201 aaagaacgtg aaatgggctg cgatcaacga ttatggcggc agttccggga cagaaactcg
     1261 tccactgcaa taaaaaatat aaaaacacag gccatcaggg aatgccacaa cgacaccacc
     1321 agggacataa acgcacaccg aaacagttgg cgctcattat caaacgttgt ttgccgatgg
     1381 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
     1441 ccattatgct ggaaactaca aaaagtggta tgcaaacaac cgatctgtca cgtttttcaa
     1501 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
     1561 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
     1621 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
     1681 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccagtaca gctgctgaat
     1741 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
     1801 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
     1861 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
     1921 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
     1981 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
     2041 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
     2101 gctacgcctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
     2161 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
     2221 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
     2281 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacac
     2341 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
     2401 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
     2461 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
     2521 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
     2581 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
     2641 ttcatggcta tcaatggcgt actcagtaca tcaaagatat cccggaaacc aacaccaata
     2701 tcgctgttag ctactatcgc tataccaacg atggctattt tagttttgat gaagccaata
     2761 cccgcaattg gggctataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
     2821 caatatttga tggggtaagt ctgtatgcct ccggttcaca gcaagactat tggggcaata
     2881 acgagaaaaa cagtaatatc tctgttgggg tttccggcca gcaatgggga attggttaca
     2941 gcctgaatta tcaatacagc cgctacactg atgaaaataa tgaccgtgca ctctctttga
     3001 atctcagtat tccgttagaa cgctggtgga tttgccccta tatttccaga catctgttat
     3061 cacttaacc
//
LOCUS       DAETQL010000218         2951 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.222, whole genome shotgun sequence.
ACCESSION   DAETQL010000218 DAETQL010000000
VERSION     DAETQL010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.222"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            141..2009
                     /gene="kup"
                     /locus_tag="K0W65_004261"
     CDS             141..2009
                     /gene="kup"
                     /locus_tag="K0W65_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBI3097257.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2176..2595
                     /gene="rbsD"
                     /locus_tag="K0W65_004262"
     CDS             2176..2595
                     /gene="rbsD"
                     /locus_tag="K0W65_004262"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBI3097258.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2603..>2951
                     /locus_tag="K0W65_004263"
     CDS             2603..>2951
                     /locus_tag="K0W65_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBI3097259.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          658 a          701 c          779 g          813 t
ORIGIN      
        1 aagggggggc tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaagcat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgctgt cgattacggg gggcgaggtg ctgtatgctg
      841 atatggggca ctttggtaag ttctctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgttgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccactgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtact gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggct ggttgccatt gagcctcggt actgtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgcaccgaag
     1681 acgcgccata tgtccataac gtccgtcggg tacagattga acaactgtcg cccactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct gcgcggcaag ctgtacttgc
     1921 tgctgcaacg taatgcgctg cgtgcaccag atcaatttga aatcccgcca aacagggtta
     1981 tcgaactggg tactcaggtc gaaatctaac gccagacgcc tcctttcttc ataagggggc
     2041 gtttttgttt tcatggttaa tcaccatgta aaacgtttcg aggttgatca catttccgta
     2101 acgtcacgat ggttttccca actcagtcag gattaagctg cggatcagcg aaacgtttcg
     2161 ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt ctgatatttc atcggtgatc
     2221 tcccgtctgg gacataccga tacgctggtg gtgtgtgatg ctggtttacc catccccaaa
     2281 agtacaacgc gtatcgatat ggcattaacc caaggtgtac cttcttttat gcaggtgctg
     2341 ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta tcgcggaaga gatcaaacaa
     2401 cataatccgc aactccacga aacgttgctc actcaccttg agcagctgca aaaacaccag
     2461 ggaaatacca ttgaaattcg ttacaccacg catgaacaat tcaaacaaca aaccgcagaa
     2521 agtcaggcgg taattcgcag cggagaatgt tctccgtatg cgaatatcat tctctgtgct
     2581 ggcgtgacgt tctgaggccg tcatggaagc attacttcag cttaaaggca tcgataaagc
     2641 cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat gtctatccgg gccgtgtgat
     2701 ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg atgaaagtgc ttactggtat
     2761 ctatactcgc gatgccggca cgcttttatg gctggggaaa gaaacgacat ttaccgggcc
     2821 gaaatcttcc caggaagccg ggattgggat tatccatcag gaactgaacc tgatcccgca
     2881 gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat
     2941 tgactggaaa a
//
LOCUS       DAETQL010000219         2944 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.223, whole genome shotgun sequence.
ACCESSION   DAETQL010000219 DAETQL010000000
VERSION     DAETQL010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.223"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..781
                     /gene="lsrF"
                     /locus_tag="K0W65_004264"
     CDS             <1..781
                     /gene="lsrF"
                     /locus_tag="K0W65_004264"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HBI3097260.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATR
                     IAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGV
                     DMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            805..1095
                     /gene="lsrG"
                     /locus_tag="K0W65_004265"
     CDS             805..1095
                     /gene="lsrG"
                     /locus_tag="K0W65_004265"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HBI3097261.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1135..1893
                     /gene="tam"
                     /locus_tag="K0W65_004266"
     CDS             1135..1893
                     /gene="tam"
                     /locus_tag="K0W65_004266"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HBI3097262.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1897..2811)
                     /locus_tag="K0W65_004267"
     CDS             complement(1897..2811)
                     /locus_tag="K0W65_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HBI3097263.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          773 a          684 c          786 g          701 t
ORIGIN      
        1 ggattgggga atgcagtcac gcttatcgtg gatatttaat ccgaaaacgg gtaaaactgt
       61 gatgctggcc tttgaccatg gttattttca ggggccgact atcggacttg aacgcattga
      121 tataaatatc gccccgctgt ttgaacatgc cgatgtatta atgtgtacgc gcggcatttt
      181 gcgcagcgta gttcccccgg cgaccaataa gccggtggta ctgcgggcgt caggtgcgaa
      241 ctctattctg gcggaattaa gtaatgaagc cgtggcgtta tcgatggatg atgccgtgcg
      301 cctgaacagt tgcgcggtgg cggcgcaggt ttatatcggc agcgaatatg aacatcagtc
      361 gatcaaaaat attattcagc tggtcgatgc cggaatgaaa gtgggaatgc cgaccatggc
      421 cgtgactggc gtgggcaaag atatggtgcg cgatcagcgt tatttctcgc tcgcgactcg
      481 aatcgccgct gaaatggggg cgcaaattat caaaacctat tatgtcgaaa aaggttttga
      541 acggattgtt gccggatgtc cggtacccat tgttattgct ggcggtaaaa aattaccgga
      601 gcgcgagacg ctggaaatgt gctggcaggc tatcgatcag ggcgcttctg gtgtggatat
      661 ggggcgtaat attttccagt ctgaccatcc ggtggcgatg atgaaagccg tacaggcggt
      721 ggttcaccat aacgaaacgg ctgatcgggc atatgaactc tatctgagtg aaaaacagta
      781 actgcggatt taaggagaag aattatgcac gtcacactgg ttgaaattaa cgttcatgaa
      841 gacaaggttg acgagtttat cgaagttttt cgccagaacc acctgggctc tgtacaggaa
      901 gaaggtaatt tgcgcttcga tgtcttacag gacccggaag tgaattcgcg cttttatatt
      961 tacgaagcct ataaagatga agacacagtg gcattccata aaaccacgcc tcactacaaa
     1021 acctgtgtcg cgaaactgga atctttaatg accgggccgc gtaaaaaacg tctgttcaat
     1081 gggttgatgc cgtgaggcta caattggggt aacgcgctga cgggagtaaa aaaaatgtct
     1141 gactggaacc cctctttata tctgcacttt tccgctgaac gatcgcgtcc ggcggtggag
     1201 ctgcttgcca gagtgccgct ggagaatgtc gaatatgttg ccgatcttgg ctgtggccca
     1261 ggtaacagca ccgccctgct gcatcaacgt tggcctgcgg ccaggataac aggcatcgac
     1321 tcgtctccgg cgatgattgc tgaagcgcgc agtgctttgc cagactgcca gttcgtggaa
     1381 gcagacatcc gcaactggca accggaacag gcactcgatc tgatttttgc taatgcctca
     1441 ctgcaatggc tgcccgacca ctacgaattg tttcctcact tggtttcttt acttaatccg
     1501 cagggcgtgc tggcagtaca gatgccagat aactggctgg agccgaccca tgtgctcatg
     1561 cgcgaagttg cttgggaaca aaactacccg gatcgcgggc gtgagccgtt ggctggcgtt
     1621 catgcttact acgatatttt gagcgaagcc ggatgtgagg tcgatatctg gcgaaccact
     1681 tactatcacc agatgccgtc gcaccaggcg attatcgatt gggtgaccgc cactggatta
     1741 cgtccgtggt tacaggatct gaccgagagc gaacagcagc tttttcttac gcgctaccat
     1801 cagatgctgg aagagcagta tccactgcaa gagaacggac agatactgct ggcatttccg
     1861 cgtctgttta ttgttgcccg ccgtacggag taattatcac gtcagctggt aatgacgatc
     1921 gggaagaatt tttgctggaa tttctgcttc atcgttcatc tgtaacaggt caatttcaat
     1981 agcgttgcag atggcatcca gcggtaaatc attgttttca gtaccgaacg gatcttccag
     2041 ttcttccgcc agacaatcca gcgaaataaa agtgtaggaa atcagcacag agataaaagg
     2101 cgtcatgtaa tgcaggtcca cgaccagcgc gaacggcagc atgatacaaa acagataaac
     2161 ggtacgatgc aaaatcaggg tgtaggcaaa gggaattggc gtgtaggcaa tgcgctcaca
     2221 gcctgccaga accgctgaaa tatcattaag ccgatcgttg aggctaataa acaggatatc
     2281 tgaaagctgt ccattgcggc gctgaaccgc caaccattct cccattatta acaagatacg
     2341 gttagctgga gagttcgaag ccagtacacg ctgaagatct ccagtcttga gataatgagc
     2401 cagcacttcc gcctgtggct gtttgcgtaa tgtcatgcgt aaacagtggg cgaaggcgat
     2461 ttgcagcctg gcaaactccc ttacacttgc cgaatccggc aatgtcgttt ttacctctcg
     2521 cagtaacgac cgtgaggcaa tcatcaactg cccccaaagt tttcgcgctt caacgtaacg
     2581 ggcgtacccg gcattattac gaaaaccaag aaaaatggcg atggcgacac cgagaatgct
     2641 gaacggtgcg agggtgaatt tgatgcccag atgcgtgtac cagggcagca tgaatataac
     2701 agcaatagaa aagagaaaat tgagtagtaa gcgcgaggat atcttggata atactgagcc
     2761 gtgccagaca aaaatacggc gcagccagtg ttgttgtgga cgaacaatca tggttatctt
     2821 caggcgtgga aaagtcgccc tattaaacgt gattacgatc acattctcaa gacgctactt
     2881 acaaattacc tagaaccacg gcagcctgac ggctgcaaat tgaaaactgc ccctgttcgt
     2941 cctg
//
LOCUS       DAETQL010000220         2933 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.224, whole genome shotgun sequence.
ACCESSION   DAETQL010000220 DAETQL010000000
VERSION     DAETQL010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.224"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="K0W65_004268"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="K0W65_004268"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBI3097264.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="K0W65_004269"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="K0W65_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HBI3097265.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..2744
                     /gene="ssuD"
                     /locus_tag="K0W65_004270"
     CDS             1599..2744
                     /gene="ssuD"
                     /locus_tag="K0W65_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HBI3097266.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2753..>2933
                     /locus_tag="K0W65_004271"
     CDS             2753..>2933
                     /locus_tag="K0W65_004271"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HBI3097267.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
BASE COUNT          660 a          801 c          805 g          667 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggggaaaccg
     2041 tcaatttcaa cggtaaacat attcatgtgc gcggagcaaa actgctcttc ccgccgattc
     2101 aacagcctta tcctccgctt tactttggcg gatcgtcaga tgtcgcccag gagctggcgg
     2161 cagaacaagt tgatctctac ctcacctggg gcgaaccgcc ggaacttgtt aaagagaaaa
     2221 tcgaacaagt gcgggcgaaa gctgccgcgt atgggcgcaa aattcgtttc ggtattcgtc
     2281 tgcatgtgat tgttcgtgaa actaacgacg aagcgtggca ggccgccgag cggttaatct
     2341 cacatcttga tgatgagact atcgccaaag cacaagccgc attcgcccgg acggattccg
     2401 taggacaaca gcgaatggcg gcgctacata acggcaagcg cgacaatctg gagatcagcc
     2461 ccaatttatg ggcgggcgtt ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg
     2521 atggtcctac ggtcgccgcg cgaatcaacg aatacgccgc gcttggcatc gacagctttg
     2581 tgctttcggg ctacccgcat ctggaagaag cgtatcgggt tggcgagttg ctgttcccac
     2641 atctggatgt cgccattccg gaaattcccc agccgcagcc gctgaatccg caaggcgaag
     2701 cggtggcaaa tgattttatc ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc
     2761 aacgccagtg aagaagtggt tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt
     2821 ggcggtgtgg caactggcct cctcggttgg ctggctgtcg acgcgtattt tgccttcacc
     2881 ggaaggagtg gtgacggcgt tctggacgct ctccgccagc ggcgaactgt ggc
//
LOCUS       DAETQL010000221         2899 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.225, whole genome shotgun sequence.
ACCESSION   DAETQL010000221 DAETQL010000000
VERSION     DAETQL010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.225"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..313)
                     /locus_tag="K0W65_004272"
     CDS             complement(<1..313)
                     /locus_tag="K0W65_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBI3097268.1"
                     /translation="MISNDILRSVRYILKANNNDLVRILALDNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DI"
     gene            complement(360..1079)
                     /gene="yehT"
                     /locus_tag="K0W65_004273"
     CDS             complement(360..1079)
                     /gene="yehT"
                     /locus_tag="K0W65_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBI3097269.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1076..2761)
                     /gene="btsS"
                     /locus_tag="K0W65_004274"
     CDS             complement(1076..2761)
                     /gene="btsS"
                     /locus_tag="K0W65_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBI3097270.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          750 a          774 c          659 g          716 t
ORIGIN      
        1 gaatgtcatc ggttttcagc gaaaacgcga tgcgtaattt tttcagcacg atgttgttat
       61 taatgcgacg ttccggctcc agtgccggag cagactcatc cttgccgcgt ttttcataaa
      121 tcaggccatt gaggaatgac gacaaaacaa tgtccggaca acgctgaaaa ccctcttcgt
      181 cttctttacg tagccagacg gcgatctgtt ccgcggtggc ttcgacatta tccagcgcca
      241 gaatacgcac caggtcatta ttattggctt tcaaaatgta gcgcacgctg cgcagaatat
      301 cgttacttat catgaggcct tcaggtgttg atgaggcaaa aagccatttt agcagtcttt
      361 tacaggccaa tcgcctcttt taagcttttc agataacggc ggctgaccgg cacggttaag
      421 ccattacgca aaatcaactc ggcctggccg ttatcttcca gacgaatctc ctgtaaatgc
      481 gcgaggttaa ccagatactg acgatggcag cgcagtagtg gtgtacgact ttccaaggta
      541 cgtaatgtca attcggtaaa gccctctttc ccttcgtggc tggtaacgta gacaccgctc
      601 atccgactgc tgacaaatgc cacatctttc atttgcagca aataaatccg actatgcccc
      661 gtacaaggga taaatttcag cgcctgttga ttttccggta acagcgaaac atcctgcttg
      721 ctgcgctcct gacgcaatcg cgccagcgtt ttctccagtc gcgcttcatc aattggcttc
      781 agcagataat caaaggcatg ttcttcaaag gctttaatag cgtattcgtc aaacgcagtg
      841 agaaaaacaa tatacgggcg atgttccggg tcaagcatcc ccaccatttc cagaccactg
      901 atgcgcggca tctggatatc gagaaacagc acatccgggc gcagtttatg caccgcgccg
      961 atcccttcca cggcgtttga acactctcca acgatttcaa tatcgctctg ctcctgcaaa
     1021 aatatacgca ggttctcccg tgctaacggt tcatcatcga caattaagac tttaatcatg
     1081 cctcgtccct ccatggtagt cgtaacgtta ttcgggtgta actatcaggc tcacaggcga
     1141 cgcttattcc atagtcatcg ccaaaccgtt cacgtaaacg cttatccacc agattcatcc
     1201 ccagcccact ggcattggtt accggttgat acaaaccggc attgtcttcg atctccagca
     1261 tcaaatgttg cccctcacgt cgggcgctga ttgccactcg ccctgtatcc agcagttgtg
     1321 atgtcccatg tttaatggcg ttttccacta tcggttgcag ggtaaacgcg ggcaattgct
     1381 gctgggataa ttcttgcgga atagcaatgt tgacctgcaa ccgcgactgg aagcgcgcct
     1441 tttcaatttg cagataagcg ttcacatgtt caatttcgtc ggcgagagta acaaactccg
     1501 aaggccgctt taagtttttg cggaaaaaag tggaaagata ctgcaccagc tggctggcct
     1561 gttcgctgtc gcggcggatc accgctttaa tggtgttaag cgcattaaac aaaaaatgag
     1621 gattcacctg ggcgtgaagc agtttgattt ctgactgggt gagcatcgct ttttgccgct
     1681 catattgccc tgcaagaatc tgcgccgaaa gcagttgcgc aatcccctcg cccagcgtgc
     1741 ggtttattga actgaataac cggtttttgg cttcatacaa tttgatggtg cccatcacgc
     1801 gctgattttc gccacgcaac ggaattacca gcgtcgaccc cagtttgcat tgcggatgca
     1861 aagagcaacg ataaggtact tcgttgccat cagcgtagac cacttcaccg gtttcaatcg
     1921 cttttaaggt gtaagtcgaa gaaatcggtt tgccgggtaa atggtggtcg tcaccaattc
     1981 cggtaaaggc cagcaatttc tctcgatcgg taatcgcgac tgcaccaata tccagctcct
     2041 gatacagtac ctgagccact ttcatgctgt tcacttcgtt aaacccctgt cgcaaaatgc
     2101 cttccgtcga ggctgccact ttcagcgcag tggcagaaaa agccgaagtg tatttttcaa
     2161 acatcgcgcg tttatcgagc aatatacgca taaacagcgc cgcgccgacg gtattggtga
     2221 ccatcattgg cgcagcaata ttactcacca gacgcaccgc atcttcataa ggtcgggcga
     2281 tcgcaaggat gatcagcatt tgcaccattt cagcgacgaa cgtgacggca ccggcggtaa
     2341 tggggttaaa gactttatca gtgcgcccgc gacggatcag gatgctgtgt accaggccgc
     2401 cgagcaatcc ttcaacgatg gttgagatca tgcaacttag cgcggtcatg ccccccatcg
     2461 aatatcgatg taagccgccg gtcagaccaa ccagcccacc gacgaccgga ccgccgagta
     2521 agccgcccat taccgcgcct atcgcacggg tattggcaat agaatcgtca atgtgcaacc
     2581 caaaccaggt gcccatgatg cagaagatgg aaaagacgat gtagcagaga aatttatgcg
     2641 gcagacgaac cgtgacctgc attaacggta tgaataatgg cgttttactc attaaccacg
     2701 caatgactaa aaaaacgcac atctgctgaa gcagcagcaa caccagatta aaatcgtaca
     2761 tacccgcaaa ccacacttcc ctttaaaacg cgtaacatac attgcctgcg tttaactttc
     2821 tttgaactct tgcagaaaaa tgagaattcg tgagtacgat cactcaaaat cgcctggcaa
     2881 aaataaaatc accctatag
//
LOCUS       DAETQL010000222         2836 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.226, whole genome shotgun sequence.
ACCESSION   DAETQL010000222 DAETQL010000000
VERSION     DAETQL010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.226"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..704
                     /locus_tag="K0W65_004275"
     CDS             <1..704
                     /locus_tag="K0W65_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3097271.1"
                     /translation="RTRETYGTRRLQTELADNGIIVGRDRLARLRKELRLHCKQKRKF
                     RATTNSDHNLPVTPNLLNQNFTPTAPNQVWVADITYVATREGWLYLAGVKDVYTCEIV
                     GYAMGERMMKELTGKALFMALRSQRLPAGLIHHTDRGSQYCAYDYRVIQEQSGLKTSM
                     SRKGNCYDNAPMESFWGTLKNESLSHYRFKSRDEAISVIREYIEIFYNRQRRHSRLGN
                     ISPAAFREKYHQMAA"
     gene            748..1548
                     /locus_tag="K0W65_004276"
                     /pseudo
     CDS             748..1548
                     /locus_tag="K0W65_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1663..2004
                     /locus_tag="K0W65_004277"
                     /pseudo
     CDS             1663..2004
                     /locus_tag="K0W65_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1987..2824
                     /locus_tag="K0W65_004278"
                     /pseudo
     CDS             1987..2824
                     /locus_tag="K0W65_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          720 a          692 c          828 g          596 t
ORIGIN      
        1 tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca gataacggaa
       61 ttatcgtcgg tcgtgacaga ctggcacgtc ttcgtaagga gctgaggctg cactgtaagc
      121 agaaacgcaa gttcagggcg accacaaact ctgaccacaa tctgccggtt acgccgaatc
      181 tgctgaatca gaactttacg cccacagcac ctaatcaggt ctgggtcgca gatataacgt
      241 atgttgccac ccgggaagga tggctgtatc tcgccggcgt caaagatgtt tatacgtgcg
      301 aaattgtcgg ttacgccatg ggagagcgca tgatgaaaga gttgacgggt aaagccctgt
      361 ttatggcgct caggagccag cgcctacctg ccgggctaat ccaccacacc gatcgaggtt
      421 cacagtactg cgcatacgat tacagggtca tacaggagca gtctggtctg aaaacatcaa
      481 tgtcgcgtaa aggtaactgt tacgacaacg ctccgatgga aagcttctgg ggaacgctga
      541 aaaatgaaag cctgagccac tatcgtttta agagccggga tgaggccatc tcagtaatac
      601 gggaatacat tgagattttc tacaatcgtc agcgtcgtca ctctcgtctg gggaatatct
      661 ccccggcagc cttcagggaa aaatatcatc agatggctgc ttaaaaaaag aacaaatggt
      721 agtgtccgct attgccagta cacctcagtt ctgggccttg cccgtagcag ttatttttat
      781 caccgggctg cactgaaagc cggtgataaa tacgccacga tacgtaagat gctgacagat
      841 atctttaaca gtaattacca gtgttatggc tatcgtcgcc tgcatgcgat gctcaggcat
      901 gagggggggc ggctatcaga aaaggttgta cgcagactta tggtggagga acagcttgtc
      961 gtcagtcgta accgtcgtcg tcgctacagc tcatattgcg gagaaatcgg accggctccg
     1021 gataacctta tcgccagaga ttttaaggcg gagcaaccta atcagaaata gctgacagat
     1081 atcacggagt tccagctccc tgcaggtaaa gtctggctgt caccagtggt ggactgcttc
     1141 gatggaaaag ttgtgagctg gtctctcagt acacgccctg atgctgaact ggtcaacact
     1201 atgctggata gcgctgtcga aacgttaaat gctggcgaac gaccggtgat acacagtgac
     1261 agaggtgggc attatcgctg gccaggctgg ctggaaagag tgaatgcagc aggtcttatt
     1321 cgctcaatgt cccgtaaagg atgttcacct gataatgctg catgcgaagg ctttttcggc
     1381 agactgaaaa cggaaatgta ttatgggcgt aaatggtcgg gcatcacgcc agaaaagttc
     1441 atgcagcaag tggatgctta catcagatgg tataacgagc ggcgtataaa attatcgctg
     1501 ggtgccgtca gccccaaaat gtaccgccaa caatgcgggc tggaatgata aagcagtcca
     1561 ggaaatcgtc cgcatccccc cttcaccatt tggattgtcc gctccaccca acatgttgtt
     1621 tccttaaggt tcctacacca caaaggaaac tctcatgctg cgcagtggat ggctgacgtt
     1681 gccgacaggc gggaacttcg ccagttcagg cagacaccgg aacagcgctt cacgcaggaa
     1741 caggagcatc tgcagccgct gctgggtacg gacttcgata tccgtcatgt ctcctgggat
     1801 ggctatatcg aggttggcgg gaaccgttac agtgtgccgg aaagcctgtg tggtcagctg
     1861 gtatcaatac ggatctcgct ggatgatgaa ctgcggatct acagtaatga gcaacaggta
     1921 gcatcgcacc gtctctgttc ggctgcatac ggctggcaga ccgtgccgga gcatcacgct
     1981 ccactctggc agcaggcttc ggagcgcatg gccgaatggc tgggggagat acagaaacgg
     2041 gtaatcactg tctgtgaccg ggaagcggat atctggcatt acctgtatta taaagtcagc
     2101 cacggacagc gtggtgcgtg ctgcacagaa tcgccggctg gaagaggcac ccggcaagct
     2161 cttcgaactg ccggaagtcc tggcaaccgc aggaagccac acgctgaatg tgatgcaaaa
     2221 aggcgggcgg gcagcccgtc aggcccggat gttcatcagt tacagcgaag tcagcataaa
     2281 aaatcccgac aacagcggcc aggcgctccc gctcacgtat gtctgttgcc gggagcaggc
     2341 agaggacggt gcctgctggc atctgctgac gtcaggaaaa gcggcgagtg ccgccgatgc
     2401 acgacgtatt gtcagccatt acgagcgacg ctggctgaca gaggaatacc acaaggcgtg
     2461 gaaaagtggt ggtacatgga atcgctgcga atgcagaccc gggataacct tgagcgcatg
     2521 gtggttatcc tggcgtttat agcggtacgg gtgctgggcc tgcggcaggg aggtgtcagt
     2581 gaagaaacac aaaatgacag ctgtgagaaa atactgacgc cgacagagtg gaaactactg
     2641 tgggttaagc tggaaggaaa gccgcttccg gtgcaggcac cgaccctgaa atgggcctgg
     2701 ggcgatggca tgacagcaaa cgcacaggtc gtcccggttg gggcgtcatg tgggatggct
     2761 ggtcagactt caggatatgg ttgagggtta cctggtgatg aagtctcttg atcaggagct
     2821 ctgatcaaga gacagc
//
LOCUS       DAETQL010000223         2806 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.227, whole genome shotgun sequence.
ACCESSION   DAETQL010000223 DAETQL010000000
VERSION     DAETQL010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.227"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            6..81
                     /locus_tag="K0W65_004279"
     tRNA            6..81
                     /locus_tag="K0W65_004279"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="K0W65_004280"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="K0W65_004280"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="K0W65_004281"
     CDS             complement(299..1057)
                     /locus_tag="K0W65_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBI3097272.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="K0W65_004282"
     CDS             complement(1065..2168)
                     /locus_tag="K0W65_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBI3097273.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGVCWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2806)
                     /locus_tag="K0W65_004283"
     CDS             complement(2178..>2806)
                     /locus_tag="K0W65_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBI3097274.1"
                     /translation="ILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLPL
                     LAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQAV
                     PYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDMV
                     SLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          763 a          739 c          674 g          630 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acagacaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaataacgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataa
//
LOCUS       DAETQL010000224         2788 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.228, whole genome shotgun sequence.
ACCESSION   DAETQL010000224 DAETQL010000000
VERSION     DAETQL010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.228"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            625..1224
                     /locus_tag="K0W65_004284"
     CDS             625..1224
                     /locus_tag="K0W65_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HBI3097275.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
     gene            1597..1980
                     /locus_tag="K0W65_004285"
     CDS             1597..1980
                     /locus_tag="K0W65_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097276.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
     gene            1977..2402
                     /locus_tag="K0W65_004286"
     CDS             1977..2402
                     /locus_tag="K0W65_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097277.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
BASE COUNT          700 a          674 c          702 g          712 t
ORIGIN      
        1 atggcttcac ccgtgttaat atcctggata actgtgtttg aaggatttct gttaataatc
       61 tgctcctgca actcacctga tgagtattag tggtaataca taatcttact cgtaaaagag
      121 tataccttat gtcattaaac agtcttccgg cgtgttgttc aatcctgtac ctctgttact
      181 tatctggtac gttgatatgt gtaatgctac gttaattgca atatctggtt atggctttat
      241 accttcagca tttaaaatat tttattaccg gagaaacagg gaagtggaaa gacttttggg
      301 ataatgtcag gccattctga cgttaatcac agtaccaaat aacgagaggg taatggaagt
      361 taaaacctgc gtaagttcct ctgttgttca ctggaaaaag tgattgccca cacgcgttat
      421 aaaggccttt ccgctgcagc ctggttaaca gagcttcagg cgttatatgc gctgtatggc
      481 tgttacgtat gctgttcttt gcctggttct gatacccgtg gaagtcgtta ccggaggaaa
      541 cacgttcacc ctactacctg agaacatctt cttcaaaaat tacggcaacg cttcttcctg
      601 tgttacagaa cacgaggaag aaacatgcaa acttataaca ccaatcccgc ttacgaaatg
      661 gctccccagc tcctggagca ctttaaccag catctggatt cattattcgg ggtctattcg
      721 aaactgttac cgttcagaat ggattttgct taccgaaaaa acaccctcag ctacagatgt
      781 gcctgcaggt acgccatgtg cgcggagatg ctgcaactga ttaacgaggt gggagagaaa
      841 ctggtcgggt atgcctgggt gatggaatat acagaacgta aagggcttca cattcatttt
      901 gtgggctatc tgaacggcca gagccacagg agttcgtatc tggtatccag attgatggga
      961 gatatctgga ggcgggttac cgagggtaat ggttactacc actggtgccg gtttaataaa
     1021 aattatccgg taaacattaa ccacgtcatt cactattcag atcacaaagc ggtgaatgat
     1081 ttacgctatg ccatcagcta cctggccaaa cgggaacaga aagaatgtgg catcatcctg
     1141 aaatgcagcg ggcttcctga aaaaagtaac cggggtcgac cacgactgga cagccccctg
     1201 ccggggatat gtgctctggt gtaaacccct gaacgacaag tacgggctgg cgctaatctt
     1261 tgctgtcggt tccggcggcc acttcagcgg cactgcgcat tttttacggc gtggtcattc
     1321 cgtatgaacc atacggagat ttaaccatga cacattcttt accattcctt ccggcaaccg
     1381 atacagcaca tcccccaatc tccatctctg cttactgact gttttttacg tcagtgggcc
     1441 attgtgtgac aaccacagtg ctgtaacagc aaccagcctg ctttacccaa actccagtcc
     1501 agattttaac tgaaccgcca ttcacaccct gaccacgctg cccgtaccgg gacggctgtt
     1561 gcctgcgtgt cgtctggcgg ctgaggaaca tatactatgt acgcaaaatc ttttctcgca
     1621 cttgatggca acggacgtct gaccggtgcc cgtaccgcac agactgcacc ttacgatagc
     1681 tacacctgcc acctttgcgg cagtgcactc agataccacc cgcaatacga cactgaacgg
     1741 ccctggtttg aacacgctga cgaagggctg acggagcacg gtcatgagtg cccttatgtc
     1801 aggccggaac gcagagaaat acggttgatt aaacgtctgc agcaattcgt cccggatacc
     1861 ttacccgtag tacgtaaagc cagctggcac tgcaggcaat gtcaccacga ttattatggg
     1921 gagcagtact gcacacactg ccagaccggg cgcttcagtg aggagggggg agcagaatga
     1981 acggtataaa aacggggctg gggatcacgc cgggtgagca cattatcagt gccgactctg
     2041 cgctcagcag aaatatccgg cagtgtttct gcctttcctg ccatggccgg ttgattctgc
     2101 agacagatgc tcagggggcg tggtttgagc atgatttaca tgccctgagt gagcaacaga
     2161 aggcggactg tgtggtgctg aatccggaga agtcccaccc ttacattgag gatatggcga
     2221 tgtttttatc gccattaccg gtcgtgctgg agtggcattg tgtgatgtgt gagcagtttt
     2281 ttcatgggaa gaagttctgt gaggcctgtg gtacggggat ttattgcaga gcggtatgta
     2341 cgaagtctgt ttacagctac cagccagact tgtttgagga ctgcggaggt gcgagtactt
     2401 aatccgaaat gcccgacctg ctcagggcgt tcagaggtat tgccaacctg tattttatct
     2461 ggtgtttcgc acccaccccg ttcgtgacac aaaggcgcga caccggatat ccgcagtcct
     2521 gcttacctga ataaaacccg gggtattgat tggtagtgtg gtgcggaatc gtaccgtgat
     2581 ctcgcattcg ccgaattcgc actgtggtgt caatcaacca tacgcgagag tgaaccgccc
     2641 cgggaatcct ggagactaaa cttcctgaga aagaggtaaa caggatgact aaaaatactc
     2701 gtttttcccc cgaagtccgt caacgggcag tccgtatggt tctggaaagt cagagcgaat
     2761 atgactcaca atgggcgaca atttgttc
//
LOCUS       DAETQL010000225         2772 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.229, whole genome shotgun sequence.
ACCESSION   DAETQL010000225 DAETQL010000000
VERSION     DAETQL010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.229"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            2..>2772
                     /locus_tag="K0W65_004287"
     rRNA            2..>2772
                     /locus_tag="K0W65_004287"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          732 a          606 c          872 g          562 t
ORIGIN      
        1 aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga tgaaggacgt
       61 gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc gatttccgaa
      121 tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg ttaatgaggc
      181 gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc
      241 cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga
      301 agcgtctgga aaggcgtgcg atacagggtg acagccccgt acacaaaaat gcacatactg
      361 tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg ggaccatcct
      421 ccaaggctaa atactcctga ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa
      481 agaaccccgg cgaggggagt gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag
      541 cacgcttagg cgtgtgactg cgtacctttt gtataatggg tcagcgactt atattctgta
      601 gcaaggttaa ccgaataggg gagccgaagg gaaaccgagt cttaactggg cgttaagttg
      661 cagggtatag acccgaaacc cggtgatcta gccatgggca ggttgaaggt tgggtaacac
      721 taactggagg accgaaccga ctaatgttga aaaattagcg gatgacttgt ggctgggggt
      781 gaaaggccaa tcaaaccggg agatagctgg ttctccccga aagctattta ggtagcgcct
      841 cgtgaattca tctccggggg tagagcactg tttcggcaag ggggtcatcc cgacttacca
      901 acccgatgca aactgcgaat accggagaat gttatcacgg gagacacacg gcgggtgcta
      961 acgtccgtcg tgaagaggga aacaacccag accgccagct aaggtcccaa agtcatggtt
     1021 aagtgggaaa cgatgtggga aggcccagac agccaggatg ttggcttaga agcagccatc
     1081 atttaaagaa agcgtaatag ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg
     1141 ctaaaccatg caccgaagct gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt
     1201 ctgtaagcct gtgaaggtgt gctgtgaggt atgctggagg tatcagaagt gcgaatgctg
     1261 acataagtaa cgataaagcg ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc
     1321 caacgttaat cggggcaggg tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat
     1381 gggaaacagg ttaatattcc tgtacttggt gttactgcga aggggggacg gagaaggcta
     1441 tgttggccgg gcgacggttg tcccggttta agcgtgtagg ctggttttcc aggcaaatcc
     1501 ggaaaatcaa ggccgaggcg tgatgacgag gcactacggt gctgaagcaa caaatgccct
     1561 gcttccagga aaagcctcta agcatcaggt aacatcaaat cgtaccccaa accgacacag
     1621 gtggtcaggt agagaatacc aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa
     1681 tggtgccgta acttcgggag aaggcacgct gatacgtagg tgaagcgact tgctcgtgga
     1741 gctgaaatca gtcgaagata ccagctggct gcaactgttt attaaaaaca cagcactgtg
     1801 caaacacgaa agtggacgta tacggtgtga cgcctgcccg gtgccggaag gttaattgat
     1861 ggggttagcg caagcgaagc tcttgatcga agccccggta aacggcggcc gtaactataa
     1921 cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acctgcacga atggcgtaat
     1981 gatggccagg ctgtctccac ccgagactca gtgaaattga actcgctgtg aagatgcagt
     2041 gtacccgcgg caagacggaa agaccccgtg aacctttact atagcttgac actgaacatt
     2101 gagccttgat gtgtaggata ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag
     2161 ccgaccttga aataccaccc tttaatgttt gatgttctaa cgtggacccg tgatccgggt
     2221 tgcggacagt gtctggtggg tagtttgact ggggcggtct cctcctaaag agtaacggag
     2281 gagcacgaag gttggctaat cctggtcgga catcaggagg ttagtgcaat ggcataagcc
     2341 agcttgactg cgagcgtgac ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg
     2401 gttctgaatg gaagggccat cgctcaacgg ataaaaggta ctccggggat aacaggctga
     2461 taccgcccaa gagttcatat cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat
     2521 cctggggctg aagtaggtcc caagggtatg gctgttcgcc atttaaagtg gtacgcgagc
     2581 tgggtttaga acgtcgtgag acagttcggt ccctatctgc cgtgggcgct ggagaactga
     2641 ggggggctgc tcctagtacg agaggaccgg agtggacgca tcactggtgt tcgggttgtc
     2701 atgccaatgg cactgcccgg tagctaaatg cggaagagat aagtgctgaa agcatctaag
     2761 cacgaaactt gc
//
LOCUS       DAETQL010000226         2713 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.230, whole genome shotgun sequence.
ACCESSION   DAETQL010000226 DAETQL010000000
VERSION     DAETQL010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.230"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            52..2574
                     /locus_tag="K0W65_004288"
     CDS             52..2574
                     /locus_tag="K0W65_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI3097278.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2585..>2713
                     /locus_tag="K0W65_004289"
     CDS             2585..>2713
                     /locus_tag="K0W65_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBI3097279.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          727 a          585 c          678 g          723 t
ORIGIN      
        1 agtgacccga taaaaatgac gctgtaagca agggataagg ataggcgtat tatgatgacg
       61 accagaatag tggttggcct cacggcaggg acgtgtctga ttttctcgca aaacctgatg
      121 gccgaggtca gtgtattcaa tccggcgctt ctggaaatca accatcaatc cggagtcgat
      181 attcgccagt ttaatcgggc aaacctgatg cccccaggtg tttatagcgt tgatattttt
      241 atcaacggta aaatgtttga acgtcaggat gtgacatttg ttcaggataa tccagatgct
      301 gatctgcacg cttgctttat tgccattaaa aaaacactgt cctcctttgg cataaaagtt
      361 gatgcgctca aatcgttcaa tgatgtggat gagacggttt gcctcgatcc tgctccacgt
      421 attgaaggct catcctggca gtttgacagt gataaattgc agctgaatat atccattcac
      481 caaatctaca tggacgcgat ggcttatgat tacatcagcc ccacgcgttg ggatgagggg
      541 attaatgcgc tcaccatcaa ctacgatttt tctggttcac atacactacg ttcagattat
      601 ggttcacaag agacagatac cagttatctc aatctgcgca atggactgaa tattggaccg
      661 tggcggctac gtaattacag tactttaaac accagcgatg gccgtgcgga atacaactcc
      721 attagtacct ggatacagcg cgatattgcc gcgttaagaa gccagattat gattggtgat
      781 acgtggacgg cgagcgatat tttcgacagt acgcaaattc gcggcgcgcg tttgtatact
      841 gataacgata tgctacccgc cagccagaat ggctttgctc ctgtggttcg tgggattgca
      901 aagtccaacg ccaccgtcat cattcggcag aatggctacg tgatttatca gtcagccgtt
      961 ccacaaggtg cttttgagat caccgatctc aacaccgcaa gtacaggtgg cgatttggac
     1021 gtaaccatca aagaagaaga cggtagcgaa caacgattca cccaacctta tgcttcattg
     1081 gcgattctta aacgtgaagg tctgacagat gttgatgtca gcgtgggtga attgcgcgat
     1141 gaagacggat ttacaccgga cgtccttcag gcgcaaatac ttcatggttt ttcccacggg
     1201 atcactttat atggaggtat gcaggctgct gaaaattatg gttctgcagc tctgggtgtc
     1261 ggtaaagatc ttggcgcttt gggcgcaatt tctttcgatg tgacacatgc tcgtgcgaat
     1321 tttagccatg atgatacaga aacgggtcag tcatatcgct ttctctattc aaaactattt
     1381 gacgacacag acactagctt gcgcctggtt ggctatcgtt actccaccga gggctactat
     1441 accctcaatg agtgggcatc gcggcgcaac agccctgaag acttttggga aacaggtaac
     1501 cgacgtagtc gcgtggaggg aacgctaacg cagtcgttgg ggagagatta tggcaattta
     1561 tacctgacat taagccggca acaatactgg cataccgatg atgtcgaacg attaatgcaa
     1621 tttggctaca gcagtagctg gaagcgtctc tcgtggaacg tctcctggag ttattccaat
     1681 actgccagac aggggacggg gaacaaccat gccagtgata acaccagtga gcagatctac
     1741 atgctctctt tatctgttcc tttatcgggc tggtggggta atagttacgc cacctattct
     1801 gtttcgcaaa acgataattc cggtagctca catcaactcg gactcagcgg tacggcgctg
     1861 gaaagaaata acctttcatg gaatttaatg cagtcctata acagtcatga tgatgaggtt
     1921 ggcggtaata tgtccctgac ctatgatggc tcttatggca cggtgaacgg cagctataac
     1981 tacagccaaa attcccagag gctgaattat ggtatcagag ggggaattct ggcacacagc
     2041 gaaggggtaa cgttaagtca ggagttaggt gaaactattg ctcttgttaa agcacctggg
     2101 gccgccaggt tagaaataga taatatgcgc ggtgctgcga cggactggcg cggctatacg
     2161 gtcaagacac agctaaaccc ttatgatgaa aatcgggtag caatcagcga taactatttc
     2221 tcgaagtcga atatagaact tgataatacc gtcgttacga tggttcccac gcgtggtgca
     2281 gtggttaaag cggagtttgt gactcatgtg ggttatcgcg ttctcttcag agtgttaaat
     2341 gcaaatggta aaccggtacc ttttggagcc attgctgcga tacaagatgc aagtttggca
     2401 gattcaggaa ttgtcggtga ccgtggcgaa ctttatcttt ctggtctacc agaaaaagga
     2461 caggttacgt tatcctgggg agaaaacgcc tcaacaaaat gcatcttcaa ttattcattt
     2521 tcgacaccag aaagtgagag cggattaatt gaacagggtg tgacatgtca ttaaaaggat
     2581 ttttatgaac aagtatatca aacagtggtg ctttgctgtg tttatgctct cgttaagtag
     2641 tgtagcactt gcagctccta aaggtatctg tacccctgat aatggcgtat ttcacagtac
     2701 gcttgatttc tcg
//
LOCUS       DAETQL010000227         2653 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.231, whole genome shotgun sequence.
ACCESSION   DAETQL010000227 DAETQL010000000
VERSION     DAETQL010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.231"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            114..1718
                     /locus_tag="K0W65_004290"
                     /pseudo
     CDS             114..1718
                     /locus_tag="K0W65_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            2540..>2653
                     /locus_tag="K0W65_004291"
     CDS             2540..>2653
                     /locus_tag="K0W65_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3097280.1"
                     /translation="MKKRNFSAEFKRESAQLVVDQKYTVADAAKAMDVGLST"
BASE COUNT          773 a          570 c          619 g          691 t
ORIGIN      
        1 gacctttctt aaagtactcc aacctactga atcctaaggg atacggcttt aagcctcctt
       61 gcagagttac ttactgtaac ccactgtgta accctctcag ggcatgcaga catatgattc
      121 agctatccag tagtcacaaa cttccagcag tctactatct ctaccagcgt aacggcgtct
      181 actacttccg tcttagggtc agacaatcaa acaatgatag aatgacctca atatcattac
      241 ggaccaagga tagacgcacc gctatggctt actcaaggca cataaaggca gcactcaagg
      301 caatacacgc agacagaccc aacgctacct atgaggaaat gagggaacac cttaaggaca
      361 tcgctgagtg tgaactgtct atgggccgtt cagatctctt tgagcctgac atgagggata
      421 tctaccggga ccaatacgga gaacttggtg agagccttac cgatgctctg gctagtgagc
      481 cgctaagcat tgaccagcat cgttacatca atgaggccct gaaggtgcta aaagcctgta
      541 tgaggcgtat cgaagcggga gacagtcagc ctctgataga ttacgtcgat ctttttaatg
      601 atattgatag gtaagataac caagcagact ctgtgtccct atctgttaat gctccagaag
      661 taaaaccaga agttacgcct agcattacca tagcgagcct ctttgaacaa tacgaagcgg
      721 agaactatca gaactggaag cctgcaacct taagggaaaa caaggcgtca cacgctgcgt
      781 taatcgaaat attcgaccat ctgggtctca atgcagacgc taacagggct gacatgctta
      841 gggtccgtga tgtgctccag cagttaccta ggaaccgtaa gcagcgcttc aaggatgtcc
      901 cacttgctga cctgttgagc cgtgaagata agacagattg tctggatgtc gttaccatca
      961 acaacaagta tcttattaag atggctgctg tctttaggtg ggctgtgcgc aatgacttga
     1021 ttaaaaagaa catgactgag ggtctggagc ttaaagtccc tcagcgtaaa gcctcagggg
     1081 cccgtaacgc cttctctacg gagcaggtgg ggcaactact ggtcgcagct aaggcatact
     1141 ctcagaagac ctctggtaag ccgtatcatt attatgttac cgccttggct gctatcactg
     1201 gggcgagact caatgaaata gcccaactac aggtcaaaga cgtcaggacc actgaggcgg
     1261 gaaccgttta tattcatatc aatgaagacg atagtagtct acccggtaag agcatcaaga
     1321 acgctcatag tgaccgctgt gtgcctctgg tcgatggggc ctatggtttt atattggctg
     1381 actttatggc gcttgtagag acccgtagag gcgctgacgg tgatgacgct atggtgttcg
     1441 atggtctgcg tctcatgaag aacggttacg gtgagcaggt gagtaaatgg tttaatcgga
     1501 cgttgttgcc taaagtcctc gtagaccgta gcggcttagc gtttcactcg tttagacata
     1561 cagttgcggc ccagttaaaa caacatgggg tcgagttggc gtatgcacag gcgatcatgg
     1621 ggcatagttc agggtctatt acgtacgacc gttatgctaa agaggtcgag gttgataggc
     1681 tggtcaatgt gatggctgat gtttataagg agacctagag gagaccatag tctaggatga
     1741 cacttactac agacctgtgt ggtcatgttt ttgactcata gttttctcta cttgtcaagg
     1801 gataattaat acgactcact atagggagaa gggacacaaa aagttttctc ttctaggatg
     1861 acacttacta cagacctgtg cggtcatgtt tttgactcat agttttctct gcttgtcaag
     1921 gggtaattaa tacgactcac tatagggaga agggacacaa aaagttttct cttctaggat
     1981 gacacttact acagacctgt gtggtcatgt ttttgactca tggttttctc tacttgtcaa
     2041 ggataattaa tacgactcac tatagggaga agggacacaa aaagttttct cttctaggac
     2101 aactaaccgt aacttatcgc atcctttcag gggcgggatg accaattaca gacaccaagc
     2161 gttctactta aagtgaatgc ttcctgtgtg ctctgggtca gtcgataagt gaatagcaat
     2221 aagacacaat catcttaatt atggtctcta gtccctacag gggcttaact taaagttaac
     2281 ttaaagacac aattaaagat taacttatag tattacttaa agttaactta aagacacaat
     2341 tagagattaa ctttaagtat taaataaaag atttaattaa agaccaatta aagattaact
     2401 ctaagggaat accttaaggg gtgatgatat ttataataat caatcaccaa acctataggt
     2461 cttctcttga agtggcacac tgaatttggc cacctgaaca gaggtgatat gctcacctca
     2521 gaacaacaca ggtgctccaa tgaaaaaaag aaattttagc gcagagttta aacgcgaatc
     2581 cgctcaactg gttgttgacc agaaatacac ggtggcagat gccgccaaag ctatggatgt
     2641 tggcctttcc aca
//
LOCUS       DAETQL010000228         2549 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.232, whole genome shotgun sequence.
ACCESSION   DAETQL010000228 DAETQL010000000
VERSION     DAETQL010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.232"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(785..859)
                     /locus_tag="K0W65_004292"
     tRNA            complement(785..859)
                     /locus_tag="K0W65_004292"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(824..826),aa:Gly,seq:tcc)
     gene            complement(863..938)
                     /locus_tag="K0W65_004293"
     tRNA            complement(863..938)
                     /locus_tag="K0W65_004293"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(903..905),aa:Thr,seq:tgt)
     gene            complement(941..1017)
                     /locus_tag="K0W65_004294"
     tRNA            complement(941..1017)
                     /locus_tag="K0W65_004294"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(981..983),aa:Arg,seq:tct)
     gene            complement(1019..1094)
                     /locus_tag="K0W65_004295"
     tRNA            complement(1019..1094)
                     /locus_tag="K0W65_004295"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1059..1061),aa:Ile,seq:cat)
     gene            complement(1271..1960)
                     /locus_tag="K0W65_004296"
     CDS             complement(1271..1960)
                     /locus_tag="K0W65_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBI3097281.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTRSEQKA
                     AFSKQSIAKVD"
     gene            complement(1957..2316)
                     /locus_tag="K0W65_004297"
     CDS             complement(1957..2316)
                     /locus_tag="K0W65_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBI3097282.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(2316..2453)
                     /locus_tag="K0W65_004298"
     CDS             complement(2316..2453)
                     /locus_tag="K0W65_004298"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097283.1"
                     /translation="MVPALRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVL
                     R"
BASE COUNT          666 a          687 c          544 g          652 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacgggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aataggggca ccccccgaca
      121 gacctgtgct atcggaaaga aaaaaggcca tcagcaaaac tctgatgacc tgaatcacct
      181 ttaccaatat tgtatgaaaa aacacgcacg actaattgac aataattttc atttccatta
      241 aaaaagatcg cgtagcattc ttaattcatg aggaacttta cccgccagca atctgagtag
      301 cgaaagctgt tcatccccaa tgtttttgct ggcgggtcct tttttctacg gtcctcctcc
      361 ccggaggggg gcatttcatt atttttctaa tcgttactgg tgagaaggcg gaagaaaaag
      421 ccagttcttc gcccctaaat aacagcatga ttccagtcca tgcaaaatct gatttccagc
      481 tcacacatac cgagcgctgt acaaaaaacc gccagccaag gcagttaaga gtgtgttccc
      541 ggggtttgct tagaatattt tgtaagttgt ccgaagagat atttacaaca ccagaatgat
      601 gaatcatcag tcccctgcca gaggttcact acgtaacctg accgacaatg caccattctg
      661 gtgctgaaaa aagagcactg gaactgcaac aaaacaacat cacagaacag gagatggtaa
      721 ggagtcagac attcccacac aatattgtgt cagtgcaaat aacaaccttc gtctcaaatc
      781 tcgctggagc gggcagcggg aatcgaaccc gcatcatcag cttggaaggc tgaggtaata
      841 gccattatac gatgcccgca tatggtgccg actaccggaa tcgaactggt gacctactga
      901 ttacaagtca gttgctctgc ctgctgagct aagtcggcgc tggcccacca ccgaggactc
      961 gaacctcgga ccgtcaactt agaaggttga tgctctatcc ggatgagcta gtggtgtttg
     1021 gtggcccttg ctggacttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     1081 tgagctaaag ggccggaggc agaataataa ccatatgtca tcacatctgc aaactcatct
     1141 gaccaccagc acgtttaacg tcctgtgcca tttttcaggc ataaaaaaac cgctcggcgg
     1201 cgggttttgc ttactttacc atcgcgtaca aaatcggcaa agtatcagat ttgcatgaga
     1261 tatatgcctt ttaatctact tttgcaatac tttgctttga aaatgccgct ttttgttctg
     1321 aacgtgttct catcacaaac aataaagcct cactatccag tcggtgaaaa atgtgtttca
     1381 ttacaaccca gtgaccagta aatgttttgg accagttttt ggttgttact cccgccagta
     1441 atgccagctc ctgatattca taaccttccc caccaaaaag ttctgctttt accgcctgcg
     1501 ccgccagcca gattaatttt ttcaggcgtt cctgtgtttt ctctgcaatt ttcctagtgc
     1561 cagaatgagc cttaaactct tcccacgccc agtgggttat ggtgatctga tactcccagc
     1621 aaatacttcc gctgtaacac cacaacaacc aggctttata gtgctcttca agagacagaa
     1681 cagcccgccg ccacgatgat gtcgaaaacg caacctgact gaccagagga attgacgccc
     1741 ccttcgccag tgattgtttt cccgggattg gtggattatc ccgcgttatc atttttccgg
     1801 tcacctcatc gcggtaccgg atttttttac gcctgtaacg ccctgtatcg aacatggcat
     1861 tctcctgcca ggcttcaagc tgaccttttg tcgccccact caaatcagcg gtggcgataa
     1921 tgagctgctc acgaacaaac tgtaaatact ggttattcat gcacactcca gctctgtgat
     1981 ttttatcccc aaccgaccac caggaacagg cagtccgcgc acaatattgg tttcatcaaa
     2041 ctgctcgtcg tcgataagca accccgcatg tgtcagtgca tccagtggtg ctttcaggat
     2101 attgtccagg tcacgacgcc gcttatccgg tggctctgca ataatcttta tcgccagtct
     2161 tccggacagg cttaatttca gtcgctgctg acggacaata agcgccacat cccggcgata
     2221 acgctcaccg gcttttgata caaaatatgt gctgccacga cgtcgccagt aagtattcac
     2281 cgtcggcgga taaggtaaaa taaactcatg gcgcatcagc gcagcacctc ctgcaccagc
     2341 ttttcaaact ttccgactct ggtttccagc tctgccacac agtccaccag ctcatctact
     2401 gctttctgtg cgcgatgctt cgcctgcatc agttcccgaa gcgcgggtac catattgagg
     2461 tagcctgagt ttaacggaca ctccttcctg aaatagaatg gcatcagaag gagccaataa
     2521 tgagcagaaa aacccaacgt tactctaaa
//
LOCUS       DAETQL010000229         2539 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.233, whole genome shotgun sequence.
ACCESSION   DAETQL010000229 DAETQL010000000
VERSION     DAETQL010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.233"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            173..1714
                     /gene="istA"
                     /locus_tag="K0W65_004299"
     CDS             173..1714
                     /gene="istA"
                     /locus_tag="K0W65_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HBI3097284.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            1729..2475
                     /gene="istB"
                     /locus_tag="K0W65_004300"
     CDS             1729..2475
                     /gene="istB"
                     /locus_tag="K0W65_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HBI3097285.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
BASE COUNT          726 a          598 c          607 g          608 t
ORIGIN      
        1 atcactcaat aacttccatc gagatcgggt aataacattt gaacagatcg ctgaataaca
       61 tcgatggaga tcacttttga ctcattttgt tattcagtga tctccatcaa tgttattgga
      121 acttcacagg tgtgttgatc tgtatctttt gccattccgg taaaggatac ctatgccaac
      181 agttccaatt tctatgagaa aacttaaaga aattcttagg cttaaatacg gtgttggact
      241 cagccatcga caaattggtc gtagtcttgc aatctcccct tccgttgtat ccagatatgc
      301 taatcgggcg gctcaacttg gcataaagca gtggccctta cctacaggat gggatgatac
      361 aaaactaaaa catgcgttcc ttcagaccca ggttaagatg aagaagcact ctctgcctga
      421 ctgggctaca gtacaccggg aactgcgtaa taaatgcgtg acgctgcagc tactctggga
      481 agaatactgt gagcgtaatc caggcggttt ttacagctat aaccattact gccggatgta
      541 ccgtgaatgg ctcaaaacca cttcaccatc aatgcgtcag gtacataaag ctggcgaaaa
      601 acttttcgtt gattactgtg gacctaccgt tggcgttacc gaccctgaga ccggagaaat
      661 aagaactgct caggtcatcg tagctgttct cggggcatca agttacacat gggcagaggc
      721 cacctggtct cagcagcttg aagactgggt gatgagtcat gttcgctgct tccagtggtt
      781 gggtggcgtt cctgaacttg ttgttccgga caatctgaaa agcgccacat ccagggcatg
      841 taagtatgat cctgacgtta accctaccta ccagcagatg cttgagcatt ataatgtcgc
      901 agttttgcct gcgcggccac gtaaaccgaa agataaagcc aaagctgaag ttggcgttca
      961 ggttgttgaa cgctggatca tggcccgaat caggcatgag atcttctaca gccttgcatc
     1021 gcttaatcag cgcattcggg agttgctgga aagactgaat aacaaaataa tgcagaagtt
     1081 gggttattca cgtgcagaac tcttcatcca gcttgataaa cccgcactga agcctcttcc
     1141 tgaagccagt tacagttaca ccctggtgaa gaaagtcaga gttcatgccg attaccacgt
     1201 ggaaatcgac aaacattact actcggttcc atgttcgctg ttaggccagc aactggaagc
     1261 atggatctcc ggagaactgg taagactctt caatcagggg caggaggttg ctgtgcaccc
     1321 gcgcaagcgt acttatggct acagtacccg caacgagcac atgcctgaag ctcatcgaca
     1381 gcatgccacc tggacgccag agcgtcttct ggaatgggcg gggcacatag gcagtgaaac
     1441 tcatagttat gtgcttcata tactgaactc tcgtccacat ccggaacaaa gctatcgctt
     1501 ctgccttgga ctcctgaacc ttcataaaaa atacagtaaa gccagactta atgcagcatg
     1561 tgcaagagct ctgaaaacaa aggtatggcg tctgtcaggt attaaatcga tcctggaaaa
     1621 aggtctggat aaacaacctg ttcaggatcc aaaaccagat ctgttatcca cgatggaaca
     1681 cgaaaacgta cgcggcagtg agtattacca ctgatacggg atccaatgat gaatcatctt
     1741 tacgaacaac tgaccgcact taaactcacc ggcttccgtg atgcgcttaa aaagcaactt
     1801 gctcagccgg gcacatacca ggagctgggc ttcgaagaac gcctgtcatt actgacagca
     1861 gaagaactaa cctgccgtga aaacaggaag gcagagcgtc tgatcaaaca tgcacggttc
     1921 agacttaatg ctgagttatc aaagctggat tatcgtaaca atagagggct ggacagggcc
     1981 ctcatccgtt cactcagtca gggaaactgg ttaaccctga aacaaaatat tttactgacc
     2041 ggggccaccg gcagcggtaa aacgttcctg gcatgtgcac ttggtcataa tgcctgccga
     2101 cagggataca aggtctacta ttatcgcctt aaagcgctga tggaacagtg ctatcagggg
     2161 catgctgatg gaagatacag caaacttttg accaggctga ataatagcga tctgctgctt
     2221 ctggatgact gggggctgga acctctctca tcagaacagc gtagcgacct gctggaaata
     2281 gtggatctga tgtaccaacg aggctcaatc atcgtagtga gccagttgcc ggtggaaaac
     2341 tggtacaaaa tgatcggaga ctccacacat gcggatgcca tcctagatcg actggttcat
     2401 ggcagtatca agatcgaact taaaggagaa tcaatgcgga aaatacaatc tccgttgacc
     2461 gaaggagatc agtgaaggta atttaaaaac ggttctgtga aagtgacacg aaccgatctc
     2521 catcgatgtt actcaccga
//
LOCUS       DAETQL010000230         2528 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.234, whole genome shotgun sequence.
ACCESSION   DAETQL010000230 DAETQL010000000
VERSION     DAETQL010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.234"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..258)
                     /locus_tag="K0W65_004301"
     CDS             complement(<1..258)
                     /locus_tag="K0W65_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097286.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            283..522
                     /gene="relB"
                     /locus_tag="K0W65_004302"
     CDS             283..522
                     /gene="relB"
                     /locus_tag="K0W65_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HBI3097287.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            522..809
                     /gene="relE"
                     /locus_tag="K0W65_004303"
     CDS             522..809
                     /gene="relE"
                     /locus_tag="K0W65_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HBI3097288.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
     gene            881..1036
                     /gene="hokD"
                     /locus_tag="K0W65_004304"
     CDS             881..1036
                     /gene="hokD"
                     /locus_tag="K0W65_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBI3097289.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1254..1505
                     /locus_tag="K0W65_004305"
     CDS             1254..1505
                     /locus_tag="K0W65_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097290.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            1572..1850
                     /locus_tag="K0W65_004306"
                     /pseudo
     CDS             1572..1850
                     /locus_tag="K0W65_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1852..>2528
                     /locus_tag="K0W65_004307"
     CDS             1852..>2528
                     /locus_tag="K0W65_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBI3097291.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLP"
BASE COUNT          654 a          595 c          663 g          616 t
ORIGIN      
        1 tgacatgcta aagcaaaaaa tagatgaata agttgagttg tgcatatgta gcctgaccgt
       61 cacaaagtat atggtgtctg taccagtaag atgatggccg gactctttaa aaaagagctg
      121 acctgcacaa tacaggatgg acttagcaat ggctgctcct ggcacaaagc ggacagtgat
      181 caccgttctt acgactactt tctgacttcc ttcgtgactt gccctaagca tgttgtagtg
      241 cgatacttgt aatgacattt gtaattacaa gaggtgtaag acatgggtag cattaacctg
      301 cgtattgacg atgaacttaa agcgcgttct tacgccgcgc ttgaaaaaat gggtgtaact
      361 ccttctgaag cgcttcgtct catgctcgag tatatcgctg acaatgaacg cttgccgttc
      421 aaacagacac tcctgagtga tgaagatgct gaacttgtgg agatagtgaa agaacggctt
      481 cgtaatccta agccagtacg tgtgacgctg gatgaactct gatggcgtat tttctggatt
      541 ttgacgagcg ggcactaaag gaatggcgaa agctgggctc gacggtacgt gaacagttga
      601 aaaagaagct ggttgaagta cttgagtcac cccggattga agcaaacaag ctccgtggta
      661 tgcctgattg ttacaagatt aagctccggt cttcaggcta tcgccttgta taccaggtta
      721 tagacgagaa agttgtcgtt ttcgtgattt ctgttgggaa aagagaacgc tcggaagtat
      781 atagcgaggc ggtcaaacgc attctctgaa ccaaagcatg acatctctgt ttcgcaccga
      841 aggtgacact tctgctttgc gttgacagga gaagcaggct atgaagcagc aaaaggcgat
      901 gttaatcgcc ctgatcgtca tctgtttaac cgtcatagtg acggcactgg taacgaggaa
      961 agacctctgc gaggtacgaa tccgaaccgg ccagacggag gtcgctgtct tcacagctta
     1021 cgaacctgag gagtaagaga cccggcgagt gagaaatccc tcgccacctc tgatgtggca
     1081 ggcatcctca acgcacccgc acttaacccg cttcggcggg tttttgtttt tattttcaac
     1141 gcatttgaag ttctggatgg tgccggaata gaatcaaaaa tacttaagta gcgcgcaggg
     1201 ataaaaggga tggaccccga acaggggagt gctacttatc tggaaggatt ctgatgatga
     1261 acatcgaaga actgcgtaaa attttttgtg aagatggcct ctatgctgtg tgcgttgaaa
     1321 atggaaatat tgttagtcat taccgcattg tgtgtttgca aaaaaatggg gctgcgttaa
     1381 ttaattttgt ggatgcccga gtgacggacg gatttatctt gcgcgacggt gagtttgtca
     1441 cttcattaca ggcattgaaa gagatcggaa taaaagctgg cttttctgct ttttcagaag
     1501 aataaactca tctacaatct tgcgcggggc tgaactcccg ctgagtaaca ccgtgccacc
     1561 ggagaaaacc gatggcacgc aacgcaaaat attacaatca tgataattcg accgttcttg
     1621 cccacacgca cgagcggtat tctcacgcat ttaagtcaga ctggtaccag catccctcat
     1681 gcactgaaga acaggccgaa tggcttattc agtgttaccg caggcgcgga tgcgaggtta
     1741 aaaaagccct tagcctcgac taccgtcact agataatctc cgtcaggctc ccttactccg
     1801 aacagccacc gcgtccgtcc cgcacattcc agcaacggat ctggaggtaa cgtgcgggta
     1861 ttacttcgac ctgttctggt tccggaactc gggctggtgg tccttaagcc cggtcgtgaa
     1921 tccatgccgg tattccacaa tacccgggta ctggtggagc cggaaccgaa aagcatgcgt
     1981 aatctgccgt ccggggtcgt tcctgccgtt cgccagccgc tagtggaaga caaaacattg
     2041 ctgccgtttt tcagtaacgc acgggtgatt cgtgctgctg gtggtgctgg cgcattgtct
     2101 gactggctgt tgcgccatat taaatcctgc cagtggccac acggcgatta tcatcacagc
     2161 gaaaccgtca ttcaccgtta tggtaccggc gcaatggtat tgtgctggca ctgcgacaac
     2221 cagctgcgtg accagacatc cgaatcactc gagcaacttg ctcatcaaaa cctgtcagca
     2281 tggatgattg acgtcatcgg tcacgcaata agcggtacgc aggagcgtga attatctctg
     2341 gctgaattat cctggtgggc ggtccgcaat caggtggcgg acgcgctacc ggaagcggta
     2401 ttacgtcgtt cgctggggtt gcgtgcggaa aaaatccgct caatgtaccg tgaaagcgac
     2461 atcgtgccgg gagagcagac cgccaccagc atactgaagc agcgcacaaa aaatcttgcg
     2521 ccgctgcc
//
LOCUS       DAETQL010000231         2498 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.235, whole genome shotgun sequence.
ACCESSION   DAETQL010000231 DAETQL010000000
VERSION     DAETQL010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.235"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(220..384)
                     /locus_tag="K0W65_004308"
     CDS             complement(220..384)
                     /locus_tag="K0W65_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097292.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="K0W65_004309"
     CDS             1286..1570
                     /locus_tag="K0W65_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBI3097293.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="K0W65_004310"
     CDS             1558..2043
                     /locus_tag="K0W65_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI3097294.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
     gene            complement(2216..>2498)
                     /locus_tag="K0W65_004311"
     CDS             complement(2216..>2498)
                     /locus_tag="K0W65_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HBI3097295.1"
                     /translation="LRASKSRSTGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQA
                     NSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT          651 a          531 c          690 g          626 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatgccc cctataaggg ctgagggatc cccacaaaat tactgctaat
     2221 aaaccagcac catatttcgg taggttctgg acagatggtt tagaactgtg ctcagtactg
     2281 ttcgtcttaa aataagtggc gagtgtcgaa gaacattctt cgctaacgtc agatatgaga
     2341 taacccgccg ggatttaatg ctgttagcct gatatttcag gtgtaaccct ttattttcag
     2401 cgtgataacc gagtaaccac attacgatag tgctcaacgt tgccagcaga ctcagaacca
     2461 gaattcttcc tgttgaacga cttttgctgg cccgaaga
//
LOCUS       DAETQL010000232         2492 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.236, whole genome shotgun sequence.
ACCESSION   DAETQL010000232 DAETQL010000000
VERSION     DAETQL010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.236"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>2492
                     /locus_tag="K0W65_004312"
     CDS             <1..>2492
                     /locus_tag="K0W65_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBI3097296.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQEQ"
BASE COUNT          730 a          578 c          602 g          582 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggagcaa tt
//
LOCUS       DAETQL010000233         2434 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.237, whole genome shotgun sequence.
ACCESSION   DAETQL010000233 DAETQL010000000
VERSION     DAETQL010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.237"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..360
                     /locus_tag="K0W65_004313"
     CDS             <1..360
                     /locus_tag="K0W65_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBI3097297.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="K0W65_004314"
     CDS             361..726
                     /locus_tag="K0W65_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBI3097298.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1406
                     /locus_tag="K0W65_004315"
     CDS             723..1406
                     /locus_tag="K0W65_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HBI3097299.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            1605..1680
                     /locus_tag="K0W65_004316"
     tRNA            1605..1680
                     /locus_tag="K0W65_004316"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1682..1758
                     /locus_tag="K0W65_004317"
     tRNA            1682..1758
                     /locus_tag="K0W65_004317"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
     gene            1761..1836
                     /locus_tag="K0W65_004318"
     tRNA            1761..1836
                     /locus_tag="K0W65_004318"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1794..1796,aa:Thr,seq:tgt)
     gene            1840..1914
                     /locus_tag="K0W65_004319"
     tRNA            1840..1914
                     /locus_tag="K0W65_004319"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1873..1875,aa:Gly,seq:tcc)
     gene            2207..2422
                     /locus_tag="K0W65_004320"
     CDS             2207..2422
                     /locus_tag="K0W65_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBI3097300.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LVLGFLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          639 a          520 c          653 g          622 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 accgccacta gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga
     1741 gtccccggtg gcggtccagt gccgacttgg ctcagtaggt agagcaactg acttgtaatc
     1801 agtaggtcac cagttcgatt ccggtagtcg gcaccatatg cgggcatcgt ataatggcta
     1861 ttacctcagc cttccaagct gatgatgcgg gttcgattcc cgctgcccgc tccagtcaga
     1921 gtctttcagt ctgcgatgat gggaaatccc ggagtgactg aaagacgttt aagttatgaa
     1981 tgatcgcttt tttttgcaaa attgctgtgc agaaatacta accttcgggc aggcgatcat
     2041 tcataagcac tctgctttta ttccgattaa ctgtgagtgg tttgttggat agagtgcttt
     2101 ccttattgta tatatcgttt cgcccgcttt tgcggttttt tcttttcaaa tccctttcat
     2161 ttctcagtgt aaaactacgc catccgttat ttgcggaggt gaggctatga aatccatgga
     2221 caaaatttca acgggcattg cctacggcac ctccgcaggc agtgctggct actggttttt
     2281 acagtggctt gatcaggtca gtccgtcaca gtgggctgcg attggtgtac tggggagtct
     2341 ggttctgggc ttcctgactt atctgacaaa tctgtacttc aaaatcagag aagacaagcg
     2401 taaggctgcg agaggtgaat aatgtcgcca tcat
//
LOCUS       DAETQL010000234         2420 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.238, whole genome shotgun sequence.
ACCESSION   DAETQL010000234 DAETQL010000000
VERSION     DAETQL010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.238"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..244
                     /locus_tag="K0W65_004321"
     CDS             <1..244
                     /locus_tag="K0W65_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBI3097301.1"
                     /translation="AENDALRRKLDNGGRVLVKGKCPVPSSAETSSASGMGNDATVEL
                     SPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(333..845)
                     /locus_tag="K0W65_004322"
     CDS             complement(333..845)
                     /locus_tag="K0W65_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HBI3097302.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1040..1804
                     /locus_tag="K0W65_004323"
     CDS             1040..1804
                     /locus_tag="K0W65_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097303.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1755..>2420
                     /locus_tag="K0W65_004324"
     CDS             1755..>2420
                     /locus_tag="K0W65_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBI3097304.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          683 a          540 c          614 g          583 t
ORIGIN      
        1 agctgaaaat gatgctcttc ggcgcaagct tgataatggt ggtcgggtgc tcgtcaaagg
       61 aaaatgccct gtgccatcct cagccgaaac ctccagcgcc tccggcatgg gcaatgatgc
      121 caccgtcgaa ctctctccag ttgctggacg aaacgttctc ggtatccggg acggaatcat
      181 cagcgaccag acagcattga gaatgcttca ggagtacatc aggatccaat gccttggggg
      241 gtagcggtaa ttttactcat tatccttcaa atcagattct gttgtcagag gaatggggga
      301 agctggattc gcaagagctt tttatgggca gtttagctaa aaaaaggcaa cacgaagcta
      361 aaactaccaa cataaagtgc tgaccagcta gcagctgaaa tcagactcac tgtatacact
      421 ttcactggat gaagttgcaa cattcctgcc accaaaggag caatgtaacg cagcacggcg
      481 ataaagcgcg aagtgaaaag tattgacaca gagttatttt gcaactgaag gcgaacccgt
      541 tctattgtat tggaacggct cgatattata catgcaagtt gagggatatg cccaattatt
      601 ataccaagat ggtaattaac gatagtaccg caccatgcgc cactcatgac cgtgattcca
      661 gcttcccatg gtgaaagagt cgtcttgctg actgtaatta cagtggccat cataattgat
      721 gctggtggca atatcgccga aatgagtaaa gtggattttg tgaacgctat tataaaaaga
      781 agcccccata ggcgatatgg atgaagtaca aaatagttca tcaaagcatt tatccactcc
      841 atcaatgtct ttccttctgt gaatttttct ttgctattta accgtgatac gacttaattt
      901 aggcttaaac accttacatt tacaaggtac tcctgtgggg ggccctgcca cggagcgtcg
      961 caggagtgag tgatgaggaa gaattaaagc aacctattgt gacaggcagc gataacagta
     1021 aatcacagga agcataatca tggcaaaacc ggactgggag gccatcgaat cggcataccg
     1081 ggccggagtc cttagcctcc gtgatatagg cgagaaatac ggcgttacag aaggggctat
     1141 caggaagagg gccaaaaagc ttgattgggc acgcagtggc ggtacgcagg tttgcaaaaa
     1201 tggtacgcaa aaaaggaaag tgcgtaccag cagaaagcct gccattactg gccttacaca
     1261 aaaaagtacg caactaaaaa cagaatctac accggatacg aaaccgatac gcggaatgcg
     1321 taccgatccc ccgactaacc cattccaacc cggtaaccag caggcattaa aacacggtgg
     1381 ttatgcccgt cgccttctgc tcaaagatga ggtgatagag gacgctaaag cgttgacgct
     1441 cgaggacgaa ttatttcgcc ttcgtgctaa caaccttgtc gctgcagaga atattggtcg
     1501 gtggttggtg tcgctggaag atgctaatgg ggaccaggaa aggaagatgc tgattgaaaa
     1561 tatcagcgcc gccgagaaag caatgatgcg caatacagtt cgtattgagt ccatcgttgg
     1621 cacgcttgcg acggtaggaa aaatatttgc tgatacagcc taccgcaagg ccgccactga
     1681 taaggtgtct ctggaggctg atcgtcttcg ccgtgatgca ggtattgatg atggcaatgg
     1741 agagcgtgac ctcaatgact tctactctga catccaaacc gacgctgaat ccggctttac
     1801 gtagtttctg gactatgcgg gcacgtaaca aagtgcttta tggtggtcgg tcatcgtcaa
     1861 aatcatggga tgccgctggc attgccatat ttctgtcgaa taaatacacc ctgcgttttt
     1921 gttgtgcccg tcagatccag aataaaatcg aagagtcggt gtataccctg ctcaaaattc
     1981 agatagacag gtttggtctg cggcaccgtt tccgtattct gaacaacaaa atcattaacc
     2041 gggttactgg ctcggaattt gttttttatg gattatggcg caacatcgaa gaaattaagt
     2101 cactggaggg gatcgatatg ttgtggctgg aagaagccca cgcactgacg gaataccagt
     2161 ggaaaattct ggagccaacg atccgtaaag agggttcgga atgctggttc atattcaacc
     2221 ccggacttgt tactgatttc gtctggcgca acttcgttgt tgatccgccc gaaggcactc
     2281 tcatccgcaa aattaactat gacgaaaatc cgtttctgtc tgacaccatg cttaaggtta
     2341 tcgacgcggc gcgacgccgt gatccggatg gttttaaaca tgtgtatgag ggcgttccgg
     2401 agtctgatga tgatgcggca
//
LOCUS       DAETQL010000235         2371 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.239, whole genome shotgun sequence.
ACCESSION   DAETQL010000235 DAETQL010000000
VERSION     DAETQL010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.239"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..823)
                     /locus_tag="K0W65_004325"
     CDS             complement(<1..823)
                     /locus_tag="K0W65_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBI3097305.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
     gene            complement(1422..1670)
                     /locus_tag="K0W65_004326"
     CDS             complement(1422..1670)
                     /locus_tag="K0W65_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBI3097306.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2025..>2371)
                     /locus_tag="K0W65_004327"
     CDS             complement(2025..>2371)
                     /locus_tag="K0W65_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBI3097307.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT          611 a          437 c          574 g          749 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagatgtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagttgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ccaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggtgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggggggc aatttttcct taaggtatga aaaaaggagc
     1081 tgaagctata taatagctat taatgccact ggtatcagtg aatcatgtat ataaaaaact
     1141 gtgttaagga ggctgtccat tagcggtttt ttattcttct ggaaccgcgg catctttgcg
     1201 tatctttttg tcttctcacc gtctggtcgg tgttttgctg agactgctaa tttcctgtct
     1261 ttgctagtgc tgtactggta ctgcccaatc atgattggcg ggggcgggcg gagttgagac
     1321 cgcagacacg tcgtatgcaa gaacgtgctg cggctggctg gtgaacttcc gatagtgcga
     1381 gtattgaatg atttccagcc gttactgatt ttacgtgcta attagtgaac aaaccactcg
     1441 tcagcagact cccaggtatc tttcagagtt tcctgaacaa aagttttagc tgaatcttta
     1501 tcggcggtgc gcgtaacaga aaggccatcg ttgctggtgg cttttacgat cacctctaca
     1561 tcgtcataac gtttactgat gcgtcgggtt aattcttcct ttaacgcatc cacagcaccg
     1621 gttggcattt tagtcatttt ctctttggct atgcagattt caaaacgcat aaaaatccct
     1681 ctgcactgtg tttttataca gtattatttt taactgtatg gataaacagt gtcaagagtg
     1741 agggaaagtg attttttggg gcatgcatgg ggcaaaaata tttgttgtgg ggcattttgg
     1801 ggcatgattg ggacactttg ttacatatga actttgccga ttttcatatg acatggcaat
     1861 ccaacgcttt gattattcta aagaaaacac atgctcttgg gcgttcttta gtgattttta
     1921 aaaatcccgt atcacgcagt taaagtggcg ggcatactct tcaaggctgg tgatccccaa
     1981 ccgcacccat ttaggatgtg accattgcgg tagaccgatg taaatcataa cgcgtgaagg
     2041 atctcttcca cgctacgcac acgggcgatg cgcgggtaga tatgattaat gctgttattg
     2101 tgttgctcgg cgctggcggc actacaggcg tcttcggcaa tcaccagatt aaagccaagt
     2161 tcccaggcat tgcgggcggt ggattcaaca ccgatattgg tcgagatccc acataacact
     2221 attgtatcga tacctcggcg gcgcagttgc aactccagat ccgtaccgta aaacgcaccc
     2281 cactgacgtt tgatgatttc gatatcactg tcagttgcac ctaatgcagc tggatgttgc
     2341 caccagtttt cgggcaacac tttagcgggg g
//
LOCUS       DAETQL010000236         2326 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.240, whole genome shotgun sequence.
ACCESSION   DAETQL010000236 DAETQL010000000
VERSION     DAETQL010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.240"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            61..282
                     /gene="kilR"
                     /locus_tag="K0W65_004328"
     CDS             61..282
                     /gene="kilR"
                     /locus_tag="K0W65_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HBI3097308.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCISDCMIKVFLGRDGLPVKAESW"
     gene            276..452
                     /locus_tag="K0W65_004329"
     CDS             276..452
                     /locus_tag="K0W65_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097309.1"
                     /translation="MVMTKKIKCAYHLCNKEIEESKIITRPLHFMRGVIPTTEMKKYC
                     SEICAEKDQMAHEL"
     gene            527..802
                     /locus_tag="K0W65_004330"
     CDS             527..802
                     /locus_tag="K0W65_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097310.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
     gene            902..2317
                     /locus_tag="K0W65_004331"
                     /pseudo
     CDS             902..2317
                     /locus_tag="K0W65_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
BASE COUNT          719 a          531 c          530 g          546 t
ORIGIN      
        1 taagtattaa ataactttcc tgagatttcc tttgtaagac tgtttattaa ggaggcttcc
       61 atgattgctc atcacttcgg aactgatgaa ataccacgtc agtgtgtgac tcctggcgat
      121 tatgttcttc atgaaggtcg gacatatatc gcctcggcaa acaatattaa aaagcgaaaa
      181 ctttatattc gtagcctgac tacaaaaaca tgcatttctg actgcatgat taaagtcttc
      241 ctcggtcgtg atggtttacc tgtaaaggcg gagtcatggt aatgactaag aaaataaaat
      301 gtgcttatca cctttgcaat aaagaaattg aagaaagcaa aatcattaca agaccacttc
      361 atttcatgcg tggagttata ccaacgacgg aaatgaaaaa atattgtagt gaaatctgtg
      421 ccgaaaaaga ccagatggca cacgaacttt aattaactga ctatccgaaa ctgaatttat
      481 gccagcaatg gcagggattc gctcaacctt aattaaggag aaaaacatga ttaccagtta
      541 tgaagccact gttgtaacta ctgatgacat tgttcacgag gttaatctgg aaggaaagcg
      601 tattggctac gtgattaaga cagaaaataa agaaacccca ttcactgtgg ttgatatcga
      661 cggtccatca ggcaacgtta aaacacttaa cgatggtgta aaaaaaatgt gtctggtgca
      721 cataggaaag aatctgcccg cagaaaaaaa agccgaattt ctggcaactc tgattgcaat
      781 gaaattaaaa ggtgaaatct gaaaaaaaga aagcctgcac actgtgcagg cctgaatgaa
      841 gaacctggga catttattca tcactcgcag taattttaat ctgagttgag gttaaaaaac
      901 aatgaacacc gataaacaag tttacccact gtattacgaa gcaaaaaatg acaaagtaag
      961 aaaacgtctc ggtattaaag gcggttttta ctgggctgaa gcgaaaaaat tatccattgc
     1021 catctcccgt ggtgctgttg cgattgacga tgcaggctac gatgaagatg actttaaaaa
     1081 acctgttcgc gtcaatttgc ccgttgttga tgacctcccg ccagaaggcg tatttgatac
     1141 ggaattctgc aaccgttacg aaaaaggcgg ggaagatggc atcacaatgg tatttatcgc
     1201 atcttctccc tctgttcagg acaaaccggc cagcactgac aataccaacg tcaacggcga
     1261 agacatgaca gaaattgagg agaacatgct cctgccagtt tccggtcagg aactgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgtttcac gcgacgaact
     1381 ccaggcatta cacattgctc gggatgaaga actaccagct gttactgccc tggctgtttc
     1441 ccacaaaacc agcctgctcg atccgctgga aattcgcgat ctccacaaac tggtgcgtga
     1501 tactgacaga gttttcccta atccaggcaa ttcaagcctg gggctgatga ctgctttttt
     1561 cgaagcatac atggacgcag actacaccga tcgcggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcacgca tcacgcgcac ggcttccggc gctaatgctg gcggcgggaa
     1681 cctcaccgat cgcggcgaag gtttcgttca cgatctgacg tcactggcgc gcgacgtagc
     1741 cactggcgta ctggctcgtt caatggacgt ggacatttat aaccttcatc cggcacacgc
     1801 taaacgtgtc gaggaaatta tcgctgaaaa taaaccgccc ttttctgttt tccgcgacaa
     1861 attcatcacc atgcctggcg ggatggatta ttcccgcgcc atcgtggttg cgtcagtgaa
     1921 agaagcaccg attggtatcg aggtcatccc cgctcacgtc actgaatatc tgaacaaggt
     1981 actgactgaa accgatcatg ccaaccctga tccggaaatc gtggatattg cctgcggtcg
     2041 ctcctctgcc ccgatgccgc aacgtgtaac agaagaagga aaacaggatg atgaagaaaa
     2101 aacgcagcca tctggcgcaa tggcagatga acaggcaacg gctgaaacaa tggaaccgga
     2161 tgcaactgaa catcatcaga acacgcagcc gctggatgct cagtcacagg taaattctgt
     2221 tgatgagaaa tatcaggaac tacgggcaga actccatgaa gcccggaaaa acattccgcc
     2281 caaaaatcct gtcgatgcag acaaattact ggctgccttg aggtag
//
LOCUS       DAETQL010000237         2278 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.241, whole genome shotgun sequence.
ACCESSION   DAETQL010000237 DAETQL010000000
VERSION     DAETQL010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.241"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            483..626
                     /locus_tag="K0W65_004332"
                     /pseudo
     CDS             483..626
                     /locus_tag="K0W65_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            773..1039
                     /gene="ospE1"
                     /locus_tag="K0W65_004333"
     CDS             773..1039
                     /gene="ospE1"
                     /locus_tag="K0W65_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HBI3097311.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            1430..>2278
                     /gene="ipaH1.4"
                     /locus_tag="K0W65_004334"
     CDS             1430..>2278
                     /gene="ipaH1.4"
                     /locus_tag="K0W65_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH1.4"
                     /protein_id="HBI3097312.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPQIFFSMGNSA
                     TISAPEH"
BASE COUNT          751 a          489 c          390 g          648 t
ORIGIN      
        1 tctcgtctgg ggaatatctc cccggcagcc ttcaggataa aatattatca gatgactgct
       61 tagaaaaaga acaaatggtg gtgtccgcta ttgccagtac acctcaagct tcttaccata
      121 tttgaaataa agctcaatag cgcgaatttt ttgttcttca gtaaacatgt ttcatcttct
      181 gagagtccag gaaatcgtcc gcatccccat taccgtctta tatcactggc gctgacatgt
      241 gctcattgat gaagtgaatc tgaatgacat gttttgccat atcaacgcca accggtgtat
      301 atttcatttg tggagtctcc agtctgagga cgctttatgc atcccgtatt gggcagtatg
      361 atgccggaaa gctgcgaggc tccacacctg ccagtttcac ccttttaacc atcatactac
      421 tccgtgttta attgtgtggg atgcgttcat tacattttaa cagtgtcacc ccgtcggcca
      481 cgaaagataa acaggtgacc agaaaaggga tcttcatcca gcacatgcta tatcttgaac
      541 gtaacaacct gaccctccgc acccgcatca agcggctggc acgtaaaaca atctgcttct
      601 cgcgttccgt ggaaatccac gaaaagtcat cggctccttc attgaaaaac acatattcta
      661 ctgattggaa gcgccacccg aaaaagtatc gtttttttac agtaaacttc atttagccga
      721 ctataatgta aaaaaataaa ttttttacca aaatataaca taaggaagta atatgcttac
      781 acaaactata ttcccttgtc ttccacaaaa acaagaaaat atcatccttg aagtatcaaa
      841 tcctgtatta ttatcttcta ctgttactac agatggatat actgttttca ataaaaaagc
      901 agctatatac gaattacaaa tacctgctgc cagccggacg aaaacgttga aattcactgc
      961 tacagaaatg caatggttga caaagataaa tgaagccgga attgatgaaa aacaaagcca
     1021 acggtattct gatttctgaa gttaatttca gactcgttta agctccattt tcaaaaaaaa
     1081 tatatcaggg tttgttacta ataacttctg aaaacagaaa aaaaatagag cagtaattct
     1141 atttcagaga ggagtaactc catctatcct cgatacgtac tgaacgttat cgtgatatat
     1201 tcatcatgct ttcttcacca ttattcgagt atagggagag acgcaatgac cttaaatacc
     1261 tttttatggc tgaattacca ttctcaggaa taacagcaga ctccttccca tagacggctt
     1321 agggggaaag caatacccgg agaaagggta caaagcaaac tgttttaaaa ttaataagcc
     1381 atattctatt taactagaat tcttatcgtt gataagaatt ctagttataa tgattaaatc
     1441 aaccaatata caggcaatcg gttctggcat tatgcatcaa ataaacaatg tatactcgtt
     1501 aactccatta tctttaccta tggaactgac tccatcttgt aatgaatttt atttaaaaac
     1561 ctggagcgaa tgggaaaaga acggtacccc aggcgagcaa cgcaatatcg ccttcaatag
     1621 gctgaaaata tgtttacaaa atcaagaggc agaattaaat ttatctgagt tagatttaaa
     1681 aacattacca gatttaccgc ctcagataac aacactggaa ataagaaaaa acctattaac
     1741 acatctccct gatttaccac caatgcttaa ggtaatacat gctcaattta atcaactgga
     1801 aagcttacct gccttacccg agacgttaga agagcttaat gcgggtgata acaagataaa
     1861 agaattacca tttcttcctg aaaatctaac tcatttacgg gttcataata accgattgca
     1921 tattctgcca ctattgccac cggaactaaa attactggta gtttctggaa acagattaga
     1981 cagcattccc ccctttccag ataagcttga agggctggct ctggctaata attttataga
     2041 acaactaccg gaattacctt ttagtatgaa cagggctgtg ctaatgaata ataatctgac
     2101 aacacttccg gaaagtgtcc tgagattagc tcagaatgcc ttcgtaaatg ttgcaggtaa
     2161 tccattgtct ggccatacca tgcgtacact acaacaaata accaccggac cagattattc
     2221 tggtcctcaa atatttttct ctatgggaaa ttctgccaca atttccgctc cagaacac
//
LOCUS       DAETQL010000238         2230 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.242, whole genome shotgun sequence.
ACCESSION   DAETQL010000238 DAETQL010000000
VERSION     DAETQL010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.242"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            651..2159
                     /gene="ltrA"
                     /locus_tag="K0W65_004335"
     CDS             651..2159
                     /gene="ltrA"
                     /locus_tag="K0W65_004335"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HBI3097313.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          572 a          548 c          657 g          453 t
ORIGIN      
        1 ggcatggatg cttaaatgaa ccgcgagtct gtctggaata ttgaaccggt aactcacgat
       61 gagaaaccca acaatcccac cgggtgtgac ggtggagaac ctgagcggca gtgacctgcg
      121 gcatgcccgc agggtgatgt aacccgctga caacggggat tgaggcgaga tcactaagcc
      181 gagatgatcc tcaaggttaa gtactgaaag gctgaagaac atgaacccgt taatccgcct
      241 ctgtgggttg aaaacgtcac cacggcctac gtgatctgac aggccgtgca ggaggaactg
      301 gcagtgatac gtaagcactg ccggtcgaag gtgttttgac atgtatgcga aacaccgggg
      361 cagcagcgtc tatcacactt gcgttgctga cttctgccaa cttgcggcaa gcaaggataa
      421 agagtgcgac gggcagcctc ctcagtatgc ctgagtccag gcaggtaaac cggggaaggt
      481 cagcgacgga tgttaagggg gcatggctcc gatgacgcgc tggctggcgg agcttccgta
      541 gtagtccgcg atggggaaag cccattacat ggcgaaggga agcagtttga atgtgtttgc
      601 gacgtgaatt aactgaccta acgaggtgaa gacctttgat aatcagcgaa atgcaacgca
      661 agcttgccac atgggcagcc accgatccgt ccctacggat tcaacggctg ctgcgtctga
      721 taacacaacc agaatggctg gctgaagcgg cgcggatcac gctttcatca aagggggccc
      781 atacccccgg cgttgatggc gtgaacaaaa caatgctaca ggccagactg gctgttgagc
      841 tgcaaatcct cagggatgaa ttactctcag gccactacca gcccttgccc gccagacggg
      901 tttacatccc taaaagcaac ggcaaactgc gaccactggg tatccccgcg ttgcgggatc
      961 gtattgttca gcgggccatg ctgatggcga tggagccgat atgggagagt gattttcata
     1021 cgctctcata tggcttccgg cctgagcgca gtgtccacca cgcgatccgc acggtgaaat
     1081 tacagctcac agactgtggt gaaacccggg gacgctgggt gattgaaggc gacctgtcca
     1141 gttacttcga caccgtacat catcgactgc tgatgaaggc tgtacgccgc aggatcagtg
     1201 acgcacgttt catgactctg ctgtggaaaa ccatcaaggc gggacatatc gatgtcggtc
     1261 tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt tatatcgccg ctattatcga
     1321 acatcatgct gaatgagttc gatcaatacc tgcatgagcg ctacctgagc gggaaagcca
     1381 gaaaagatcg gtggtactgg aataacagta tccaacgggg ccgaagtacg gcggtcagag
     1441 aaaactggca gtggaaaccc gcggtggcgt actgccgcta tgccgatgat tttgtcctca
     1501 tcgtcaaagg caccaaagca caggcggaag ccatcaggga ggagtgtcgg ggtgtgctcg
     1561 aaggcagtct gaaactcagg ctgaacatgg ataagactaa aatcacccat gttaatgacg
     1621 gctttatctt tctggggcac aggatcattc gcaaacgcag tcgttatggc gagatgcgag
     1681 tggtctcaac gatcccgcag gagaaagcca gaaacttcgc cgcatcgctg acagcactgt
     1741 tatcaggcaa ctacagtgaa agcaaagtcg atatggctga acaactcaac cgaaaactga
     1801 aaggctgggc catgttctat cagttcgttg attttaaggc caaagtcttc agttatatcg
     1861 accgtgtcgt gttctggaag ctggctcact ggctggcccg caaataccgt acaggtatcg
     1921 cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg tcagagcaaa acgtgggttt
     1981 tatttggtaa aaccaatcac ggcaagctca gcggcgaaat actgtaccgg ttggtggggc
     2041 aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa tccctatctg aggacggaga
     2101 ccagaaacac gtatacatcg cgctttacag aagtggcaat ggcgttcgcc agcatttaaa
     2161 tggagagccg gatgcgctga aaggtgcacg tccggttcgg ggaggagagg cagggaaata
     2221 gtccgactac
//
LOCUS       DAETQL010000239         2225 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.243, whole genome shotgun sequence.
ACCESSION   DAETQL010000239 DAETQL010000000
VERSION     DAETQL010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.243"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            249..1286
                     /gene="curA"
                     /locus_tag="K0W65_004336"
     CDS             249..1286
                     /gene="curA"
                     /locus_tag="K0W65_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HBI3097314.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1484..2149
                     /gene="mcbR"
                     /locus_tag="K0W65_004337"
     CDS             1484..2149
                     /gene="mcbR"
                     /locus_tag="K0W65_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBI3097315.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          612 a          471 c          599 g          543 t
ORIGIN      
        1 cgttcgagca ttataacgaa tggcatccgc atagtgcgct gggttatcgc tcgccacggg
       61 aatatctgcg gcagcgggct tgtaatgggt taagtgataa cagatgtctg gaaatatagg
      121 ggcaaatcca acggattcct cgatgaaatt attgcgtgtt gcagagggga tgtgacggct
      181 gcaaacaaaa ccaatcccgt gagcctgcta ctattgatgg attcatgcca aaagcacagg
      241 agagcattat ggggcaacaa aagcagcgta atcgacgttg ggttctggcc tcgcgtccac
      301 atggcgcacc ggttccggag aatttccgtc ttgaagaaga tgatgtcgcc acaccgggtg
      361 aaggacaggt gttactgcgc acagtttatt tgtccctgga cccgtatatg cgtggacgta
      421 tgagcgatga gccatcttat tcaccgccgg ttgatattgg cggcgtgatg gtcggcggta
      481 cggtgagccg tgtcgtggag tcgaatcatc ctgattatca gcctggcgac tgggtgctgg
      541 gctacagtgg atggcaggac tatgacatat ccagtggtga tgacctggtg aaacttggcg
      601 atcatccgca aaatccatcg tggtcgctgg gtgtgctggg gatgccaggc tttaccgctt
      661 atatggggct gctggatatc ggtcagccta aagagggcga aacgttggtg gtagctgcgg
      721 cgacagaacc agtgggggcg acggtggggc aaatcggcaa acttaaaggt tgcagagtgg
      781 tgggggtagc cggtggcgcg gaaaaatgcc gccatgctac cgaggtgtta ggcttcgatg
      841 tttgccttga tcaccacgcg gacgattttg ccgaacaact ggcgaaagcg tgcccaaaag
      901 gtattgatat ctattatgaa aacgtgggcg gtaaggtatt tgatgcagtg ctaccattac
      961 ttaatacatc tgcgcgcatt cccgtttgtg gcttagtgag cagctataac gctacagagc
     1021 taccacccgg tccggatcgt ttacccctgt tgatggcaac ggtgctgaaa aaacgtattc
     1081 gcttgcaagg gtttattatc gctcaggatt atggtcaccg catccatgag tttcagaagg
     1141 agatggggca atgggtgaaa gaggataaaa tccactaccg cgaagatatt actgatggtt
     1201 tagagaatgc gccacagacg tttatcggcc tgctgaaggg taaaaacttc ggcaaagtgg
     1261 tgatccgcgt cgcgggtgat gattaaagtt tcaacggcgg cgtaagccgc cataaaaatc
     1321 caggcaaaaa gcttattttc agctttaatt aactaaccac gtcattgttc tgctgctaat
     1381 aacaacaaag gtgggtggtt tatgattatt cactttaata cactaggtga actccttctg
     1441 ccatgcaggc agggttggac agaaaacacg cgaaggaatt aaaatgcctg gaacgggaaa
     1501 aatgaaacat gtcagtttga ctctgcaggt tgagaacgac ctgaaacatc agcttagtat
     1561 tggcgcatta aaacctggcg cacgcctgat tactaaaaat ctggcggagc aattaggtat
     1621 gagtattaca cctgtgcgtg aagcattatt acgtctggtt tcggtgaatg cgctttctgt
     1681 cgcacctgca caagcattta cagtaccgga agtgaggcaa agtcagttag acgaaatcaa
     1741 tcggatccgc tacgaactgg aattaatggc agttgccctg gctgttgaaa acctctcctc
     1801 gcaagacata gcagaacttc aggaattgct cgggaaatta cagcaggcgc aagaaaaggg
     1861 cgacatggaa caaatcatta atgtgaacag gctatttcgc ttagcgattt atcatcgctc
     1921 aaatatgccc atcctgtgtg agatgattga gcaactgtgg atcaggatgg gacctgcttt
     1981 acattatctt tatgaagcga ttaacccagc ggaattacgg gagcgcatag aaaactatca
     2041 tctattactc gctgcgttaa aagcaaaaga caaagaggga tgcaaacatt gtcttgctga
     2101 aattatgcaa caaaatattg ctattttata ccagcaatac aatcgttaaa ttaaatgccc
     2161 ggcaaatacc gggcatcaag taattactca aatcgccacg caatattcat gccgacacca
     2221 taatt
//
LOCUS       DAETQL010000240         2218 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.244, whole genome shotgun sequence.
ACCESSION   DAETQL010000240 DAETQL010000000
VERSION     DAETQL010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.244"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="K0W65_004338"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="K0W65_004338"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBI3097316.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="K0W65_004339"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="K0W65_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HBI3097317.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..>2218)
                     /gene="arsR"
                     /locus_tag="K0W65_004340"
     CDS             complement(1881..>2218)
                     /gene="arsR"
                     /locus_tag="K0W65_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HBI3097318.1"
                     /translation="IQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISRH
                     LALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQNC
                     SGDSKNICS"
BASE COUNT          589 a          610 c          555 g          464 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac taggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatgg
//
LOCUS       DAETQL010000241         2199 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.245, whole genome shotgun sequence.
ACCESSION   DAETQL010000241 DAETQL010000000
VERSION     DAETQL010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.245"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            441..1154
                     /locus_tag="K0W65_004341"
     CDS             441..1154
                     /locus_tag="K0W65_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097319.1"
                     /translation="MSQQVVIFNNEFSMSSYEFLIEVVNPARAEAGEAPVRNDQFLAR
                     VRDELDDCKTFATERITPRGGGRQMESITLTGDQLLLVGMRESKAVRRKVLDYIRRIE
                     KDKQLLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKNPISNE
                     WSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPTTVDKLRGILIAFTGTD
                     QQKQEHLLKQAQSGKLEGL"
     gene            1471..1962
                     /locus_tag="K0W65_004342"
     CDS             1471..1962
                     /locus_tag="K0W65_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097320.1"
                     /translation="MLFNNQDWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRIEAERITKELASQKE
                     RTREMFMTEAEWQKELQRREQVKTERRTYGENLRSATHSAGRSKASIMADLDSGANWM
                     DSI"
     gene            2027..>2199
                     /locus_tag="K0W65_004343"
     CDS             2027..>2199
                     /locus_tag="K0W65_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097321.1"
                     /translation="MTLTKDALQFGIEPVRITDTDNLQINEGLPTNADPQVYAFQLAK
                     LVKTMLNSVLKDA"
BASE COUNT          566 a          585 c          534 g          514 t
ORIGIN      
        1 ccatacttcg ggtctaactg gaggtcagat tatggcctta tcaggtagca aatcttatac
       61 cctcactata gggaatggac ccttagctat cactataggg accaacattc cgcctctggt
      121 cttgatgcgt caagttgtgc ggaagctctc ccgacattaa gtctttctcc ataccaaggc
      181 tagcaagaca gcctctgtca gactcccagc ccgttagcgt tctcgtgtct tcattttgac
      241 ttgggtctat cgccgctcac catctggacg tcggacatgc tgtcttgcac ctttatcact
      301 actggtcatc gcagaccaca tcacaattac ctcaagtcat tctgtttgcg tcctgttgcc
      361 tgctggtctc cctttacgtg ggggcttgag taggtcagta gggtgcgact tcagggtgca
      421 aacaaaagga gtacacgtaa atgtctcaac aagtagtcat ctttaacaat gaattctcta
      481 tgtcttccta tgaatttcta atcgaggttg tcaaccctgc ccgtgcagag gctggcgagg
      541 ctccggtccg taatgaccaa ttcttagccc gtgtccgcga tgagctggac gattgcaaaa
      601 cttttgcaac cgaacggata acacctagag gaggtggtcg ccagatggaa tcaatcacgc
      661 tgaccggaga ccaattgtta ctggtcggca tgcgcgagtc taaagctgtg cgtcgcaaag
      721 tccttgacta catccgacga attgagaagg acaagcagtt attggaagac cagaagaagc
      781 gagcagccat tcagtctgct aaccgtcgag gcgtgacttg gggggactac tgcaaaacct
      841 atggtctgcc cgctcagaag ctaatgaccg cacttcttca gcatcgtggt ctctttcgta
      901 agaacccaat cagtaacgag tggtctgtaa atccaaagta ttccgactgc ttccgtatca
      961 ttaagccgtc agaccagaag ttctcagcag gtgggtataa cttccggttc aacgcaaagg
     1021 gactggaggt cttcggtaag ccgacgacag tcgataaact gcgaggcatc cttattgcct
     1081 tcactggcac cgaccagcag aagcaagagc acctcctgaa gcaggcccaa tccggcaagc
     1141 tggagggact ctaagatgat tgacccgcgc acacctgagg gccgcatgac tctccgttac
     1201 cgtggctacc ggactgaagt catcctgaag gagcttggtc tagacccgga agatgagacc
     1261 cgccagcacc aatcacgaga cgagctaata gcccaactgg tcgctatgaa actgtcgtcg
     1321 gctagcaagc tagcgccagc acatcgttag tagctcacct acttaatacc taattttaaa
     1381 ccttttgaga tgaccgatgc ggggagacgc cgcaggacta aacacgcctg tagcagaccg
     1441 atagtctcag actcaagagg agtaattact atgttattca ataaccaaga ctggaaactg
     1501 tcagtcaccg acatcaatct ctacgagaat actgtgagtc tggacggtca gtcctatccg
     1561 ttgtcgctcg ctatcaagac cctgattccg ggctacttgt ccggcctgcc gtctaccagc
     1621 cgcgaggcaa tggagttact ggaagctctg gctgaagctg gcgtgaccat tggtaacttc
     1681 ttcagtaatg acctgatgac tgcctaccaa cgccgccaga tgaataaacg tatcgaggct
     1741 gagcgtatca ctaaagagct ggcgtctcag aaagagcgta cccgcgaaat gttcatgacc
     1801 gaggccgaat ggcagaaaga gctacagcgc cgcgaacagg tcaaaactga acgccggacc
     1861 tatggtgaga acctccgaag cgctacccat tctgctggtc gttccaaggc ttcaattatg
     1921 gctgacctcg atagtggcgc taactggatg gattccatct aagaacacac ggtccctgac
     1981 ttaccgttag ggacttcaaa tatctgactc aaggagatta attatcatga ctctgactaa
     2041 agacgcttta cagtttggca ttgagcctgt tcgtatcacc gacaccgaca accttcagat
     2101 taacgaaggt ctgccgacca acgcagaccc gcaggtctat gccttccagt tagctaagct
     2161 ggtgaagacc atgctcaatt cagtgcttaa agacgctca
//
LOCUS       DAETQL010000242         2184 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.246, whole genome shotgun sequence.
ACCESSION   DAETQL010000242 DAETQL010000000
VERSION     DAETQL010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.246"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..695
                     /locus_tag="K0W65_004344"
                     /pseudo
     CDS             1..695
                     /locus_tag="K0W65_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            697..1302
                     /locus_tag="K0W65_004345"
     CDS             697..1302
                     /locus_tag="K0W65_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HBI3097322.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1289..>2184
                     /locus_tag="K0W65_004346"
     CDS             1289..>2184
                     /locus_tag="K0W65_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3097323.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRV"
BASE COUNT          531 a          507 c          610 g          536 t
ORIGIN      
        1 gcccgtgccg gtgtgccggg cagcgatgag gaaacccgtg cggactttga aaaccgccga
       61 cgtaattcag ttgcccgtaa tgcccggaat attctggaag ccatccgggg tgaaatactc
      121 tccacggtag aaaacgtggt ggatgtttac gtcacccata atccgaaaaa aacagaacaa
      181 aaagccggag tcagtatcca ttaacacccg gttcgcttta tgttggcgtg tacggcggca
      241 gcccggcaga tatcgcggcg gccatctggc gtaaggctcc gccgggtatt gatatgaatg
      301 gcaacacaac gttcactgtt gcagataagg agtacgatcc gccgtatcct gaatacgtga
      361 tcagctggca gacactcaaa cccgtcagtc tgcatgtcag tgtgacgctg aaaaaaagtg
      421 actacctgcc ttcagatatt acccgacagg tacagcaatc cgtgctggac gcatttaatg
      481 gtacagatgg tggtctgcgg gcaagagttg cctctgttgt ctctgccggg cgctactatg
      541 ccggcattta caaaaccgat ccggaacata ttgatattct gggccttact gtgagccgtg
      601 atggttcgtc atggacaacg gctgtcactt tcgggataga tgagattccg gttctggatg
      661 tgtcggatat cagtgtgaaa ctccaggagg cataacgtgc agaatgtggc tgccactgtg
      721 cttgcgcagt atgctgccag cccccgactc aatgccctca ttaacagctt taacgcagcg
      781 ctttcccccg acagttttat cagtgatttt tatggtctta tctggaacat cgataccgca
      841 gaaaagtatg gtcttgatgt ctggggaaag attgtgggtg tcagtcgctg gctgacggta
      901 aaggacgatt ttaattacct gggcttcagc gagtccagga tggacacccc ggtaatggat
      961 gatccctgtc cgtttaatca ggcaccgttt tacaacggaa aatcggatac ccggactgtt
     1021 gacctgtctg atgctgtata ccggcggctg atactgatga aagccatgtc gaacattact
     1081 gactgttccg ttccggatat taacctgatg ctgagattta tgttcggaaa aaaacgccgg
     1141 gcttatgttc tgaataatgg cgggctgagg atgagttacg tctttgagtc cgcgctctcg
     1201 ttggcagaac tggcgattat ccagtcgtcg ggtgcactgc catccccgcc gggtgtttat
     1261 gtttcagtag ttttaaagga gtcccgtaat gaaggccagt gataaacccc gccagctggc
     1321 ggtccccttt gcgagtaccg gagataaaaa ccgtatcccg gacaaggcga cacagcagac
     1381 cagagagagc ggtaatgctg cgtatgattc aggttttcct ccggtgacca tgacagcggt
     1441 ctcagcggga ggtataccgc cacacggcaa ggattttaac ggtctgatgt acgatattac
     1501 cgcagcaata cggttcgccc aggctggcgg tttgtacacg tataatgccg gttttgcggg
     1561 ggccattggt ggatatgcaa aaggagccat tctcgccgga gtcgcaacaa cagcggtctg
     1621 gctgaatacc acggacgata acctgaccga tcctgaaggc tccgacagtg cgggctgggt
     1681 aaatcttctt gaggatccga aaaggatatt cctgcggcag aagaacaatc tgtcagacct
     1741 tcagaataaa gggacggcac gggataatct tcaggtttac agtaaagagc agtcagatca
     1801 acgctatgtt catcgggaag gcgataaaat aaccggagag ctgaaaatcc gtggtgttaa
     1861 tgcgctgagg attttcaacg aagcttttgg cctgattttt cgtcgttcgg aagagtgcct
     1921 gcaccttatt cccaccagtg aaggtcaggg ggaaaatggc gatattggtc ccctgcgccc
     1981 gttcaccatt aatttgcgga cgggtgaaat atccatgtcg cataaagtgt ctgttggcgg
     2041 tggttcgcag gtcaatggtg cgctgggtat cggcgttcag aacgccctgg gggggaattc
     2101 aattgttctt ggtgataatg acaccggatt taaacagaat ggagacggta ttctggatgt
     2161 ttatgctaat agtcagcgtg tatt
//
LOCUS       DAETQL010000243         2182 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.247, whole genome shotgun sequence.
ACCESSION   DAETQL010000243 DAETQL010000000
VERSION     DAETQL010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.247"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..388)
                     /gene="istB"
                     /locus_tag="K0W65_004347"
     CDS             complement(<1..388)
                     /gene="istB"
                     /locus_tag="K0W65_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HBI3097324.1"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVR"
     gene            complement(385..741)
                     /locus_tag="K0W65_004348"
                     /pseudo
     CDS             complement(385..741)
                     /locus_tag="K0W65_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            821..2014
                     /locus_tag="K0W65_004349"
     CDS             821..2014
                     /locus_tag="K0W65_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HBI3097325.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGFGLRASKSRSTGRILVLSLLATLSTIVMWLLG
                     YHAENKGLHLKYQANSIKSRRVISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRN
                     MVLVY"
     gene            complement(2034..2182)
                     /locus_tag="K0W65_004350"
                     /pseudo
     CDS             complement(2034..2182)
                     /locus_tag="K0W65_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          567 a          507 c          540 g          568 t
ORIGIN      
        1 cacggactgc ttcatagccc atcgctattg ccagatgggt tttccccaca cctgatggcc
       61 ccagtaatac gatattttca ttacgttcta tgaagctgag tgagcgtaac gactggagtt
      121 gcttctgcgg tgctccggtg gcgaatgtga agtcatactc ttcgaacgtt ttcaccgccg
      181 ggaaggctgc cattcgggta tacatcgcct gtttacgttg atgacgtgcc agtttttctt
      241 catgaagcag atgctccagg aagtccatat aactccattc ctggtctact gcctgttgtg
      301 acagcgcagg cgctgcgctt ataaggcttt ccagttgcaa ctgcccggcg agcgccatca
      361 gtcgttgatg ttgcagttcc atcatcacgc cactcctctg cagaatgagt cgtagatgga
      421 gagtggatga tgctgggggg ggttgtcgaa gctcaccaga ttttcaccag gatgcacgtc
      481 atactctttt ttctccggag gcagtgccag catggactgc tgctcttcga gccagcgatc
      541 acagggacgg gcctggattg tttcatgctt tcgttggtta gcgacatcgt gcagccagcg
      601 cagaccgtgg cggttggctg tttcaacatc gacagtgctc cccatcgggc gcaggcgagt
      661 cattagtggg atgtaaaaac tgttacgggt gtactgcacc atccgttcca ccttaccttt
      721 agtctgtgcc ctgaagggct gaggaatccc cagaaaacga gacaaacata gcattatgtg
      781 atctactgat ttgtgccaac aattcaatga gatcactgct atgcctgttc ataaagtatg
      841 tcagaatttc ttccgggatg ccttagcacc atttcatcag taccgacaaa atgccctgat
      901 ggatgccaca atggcgttaa tcaacggagc atctctgacg cagaccagta tcggacgttt
      961 tttgccgggt aatgcacaag ttaagaataa gatcaaacgt attgatcgcc tcatgggtaa
     1021 tgaggcgctc catcgcgata ttccaatgat tttcaggaat atcacttcaa tgctgacacg
     1081 gcagttatct ttatgtgtaa tagccgtaga ctggagtggc tatccctccc aggagcatca
     1141 tgttcttcgt gccagccttc tgtgtgacgg gcgttccatt ccgttattga gtaaggttgt
     1201 tccttctgaa aaacaaaata atccattgat acaacatgat ttccttgatt cccttgctca
     1261 atccctgccc cccgatgcca gggttattat tgtcacggat gctggatttc agagcgcatg
     1321 gtttcaccat attacttctc tgggctggga ttttattggt cggatcagaa ataacgttca
     1381 gtattgcctc gataacgcgc ctgaaagatg gttaaaagtg tcggatagcc ctgaatgtaa
     1441 aacaccggaa tatatggggg ctggaaggct ggttaaagaa aggaaaaaaa gcatccgggg
     1501 gcatttttac acctataaga aatcggcaaa agggcgaaaa aagaaacgtt cgaaaggtca
     1561 gagtggttta aacaaaacag acaaagaaca gagcaaatca gcaaaagaag cctggttgat
     1621 cttcagcagt acaaatgact tcagagcgcg agaaatcatc aagttataca gtcggcgaat
     1681 gcagatagag caaaactttc gtgatgaaaa gaacgggcgc ttcggatttg gtcttcgggc
     1741 cagcaaaagt cgttcaacag gaagaattct ggttctgagt ctgctggcaa cgttgagcac
     1801 tatcgtaatg tggttactcg gttatcacgc tgaaaataaa gggttacacc tgaaatatca
     1861 ggctaacagc attaaatccc ggcgggttat ctcatatctg acgttagcga agaatgttct
     1921 tcgacactcg ccacttattt taagacgaac agtactgagc acagttctaa accatctgtc
     1981 cagaacctac cgaaatatgg tgctggttta ttagcagtaa ttttgtgggg atccctcagc
     2041 ctgaaggggc gacacagtcg gggagagaag cccatctcct tgccgaactg ccacagcgaa
     2101 ggatggaacc ggtgctgacc ggtctgatat gcgtcacgtt gcagaaccac agttttcata
     2161 ttgtcataca acacttcgcg cg
//
LOCUS       DAETQL010000244         2177 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.248, whole genome shotgun sequence.
ACCESSION   DAETQL010000244 DAETQL010000000
VERSION     DAETQL010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.248"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(17..286)
                     /locus_tag="K0W65_004351"
     CDS             complement(17..286)
                     /locus_tag="K0W65_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBI3097326.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            343..1011
                     /locus_tag="K0W65_004352"
     CDS             343..1011
                     /locus_tag="K0W65_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBI3097327.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            1327..>2177
                     /locus_tag="K0W65_004353"
     CDS             1327..>2177
                     /locus_tag="K0W65_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBI3097328.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIW
                     HAFEHEE"
BASE COUNT          660 a          483 c          407 g          627 t
ORIGIN      
        1 ggtttttctt tttcgcttac attgtgtctg gttcttccgg ccactcaata tcaggtgcag
       61 ttgatgtatc aacacggttc agcaacaccc gatacttctt ccaggcttcc agcaacgagt
      121 tttcttcctc cgttgcgatc tccagatcta cagcatcctg aagtggcgca atatgctcac
      181 tgaatccctg gatgtagaac tgtgtggtga cggtcttcca gccattcggc tcctgctgta
      241 tcgaagcata ccaggctatt tcaatatcgc tatgctgcgg cagcatttaa ccccttgtaa
      301 ttcatcgcca taattgattt aattcacaaa taaaactata atatggtgaa attaatgaaa
      361 aaaaacacag atgatggggc taaaatttac acaccactta ccctaaagct ttatgactgg
      421 tgggttttgg gagtatcaaa tcggcttgca tggggatgtc ctacaaagga acaccttctt
      481 ccacactttc tggaacattt aggtaacaac catctggata ttggtgttgg aactgggttt
      541 taccttactc acgtacctga gagtagtctg atatctttaa tggatttgaa cgaagctagc
      601 ctgaacgcgg catctacaag ggctggggaa tcaaaaatta aacataaaat tagccatgat
      661 gtttttgaac cttatcctgc ggcgttacat ggtcaatttg attccatttc catgttttac
      721 cttcttcact gcctgcctgg aaatatatct acaaaaagct gtgtaatacg caatgcggcg
      781 caggccttaa ctgatgatgg aactctatac ggagccacaa ttcttggcga tggagttgtg
      841 cacaatagct tcggtcaaaa actgatgcgc atttacaatc agaaaggcat cttttcaaac
      901 acaaaagatt ccgaagaagg cttaacacat atactctcag agcatttcga gaatgttaaa
      961 accaaggttc aaggtactgt agtaatgttt tccgcttcag ggaaaaaata gcatccaacc
     1021 gcagcacgtt cttgcttaag acgtgctacg gtttccttta cgatttgggg ttgcgacttt
     1081 acctctattg ataatgcatt ccggcagaac gttcaaatat gaacgtacga tatttaacta
     1141 accgaaaaac aaaatatagc acaggcgaga catttaccat ctaagaaaat agtatcgttt
     1201 ttttacagtt aaatcaacat cacttcctta aaatgaaaac aataaatttc aatcctgaaa
     1261 cattttattt cagcttatta ttccccagta tcacatttta tcaggatatc cttctgcgag
     1321 gttattatgc ttcctgtaaa taatcccccc ctatccactg gaaacgtctc tttttacaga
     1381 actacatcaa tcgacaatgt tcacaataat tatctctccg aatgggttga atggactaaa
     1441 aacagcattt ccggagaaaa cagggaaact gcttttaccc ggctccaatt atgtctggag
     1501 aacagtgaaa catcgttgga cttatcttgt ttaggtctca gatctctacc acgattgcct
     1561 gacaatcttg atgaaattaa tgtaagcaat aaccaactat caatgctccc cgagctacca
     1621 agggcattga aagagctgaa tgcaagcagt aatcaattat ctgcacttcc tgaattacca
     1681 gtgtcgctgg aatatataaa tgtgagtgat aaccatttgt tcgcacttcc tgaattacct
     1741 gcgtcactag aatatattaa tgtaagtgac aatcacctgt ctgtacttcc gaggttacca
     1801 atgtcattgg aattacttga tgcagccaga aatgctttgg aagtaatacc agattttcca
     1861 gaaagagatg atcatattat aagaatattc tggcttaatc agaaccggat cacggcaatt
     1921 ccggaaagca tacttggcct cagttctgat agcgttgtca atcttagaga aaatcaacta
     1981 tctcccagaa taatgcaaac tttgttacaa caaaccgccc aaccggacta ccacggccca
     2041 cggatttact tctccatgag tgacggacaa cagaatacac tccatcgccc cctggctgat
     2101 gccgtgacag catggttccc ggaaaacaaa caatctgatg tatcacagat atggcatgct
     2161 tttgaacatg aagagca
//
LOCUS       DAETQL010000245         2164 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.249, whole genome shotgun sequence.
ACCESSION   DAETQL010000245 DAETQL010000000
VERSION     DAETQL010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.249"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            155..340
                     /locus_tag="K0W65_004354"
                     /pseudo
     CDS             155..340
                     /locus_tag="K0W65_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(440..649)
                     /locus_tag="K0W65_004355"
                     /pseudo
     CDS             complement(440..649)
                     /locus_tag="K0W65_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683482.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
     gene            complement(699..878)
                     /locus_tag="K0W65_004356"
                     /pseudo
     CDS             complement(699..878)
                     /locus_tag="K0W65_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1402..1686
                     /locus_tag="K0W65_004357"
     CDS             1402..1686
                     /locus_tag="K0W65_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBI3097329.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            1686..1961
                     /locus_tag="K0W65_004358"
     CDS             1686..1961
                     /locus_tag="K0W65_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBI3097330.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          600 a          537 c          521 g          506 t
ORIGIN      
        1 tacggtggtc ggtttacgct taccgcagga caggtggtaa ctgaaaaata gtttgctgag
       61 gcgactggaa acagaagggc aagatcgatt ccgacagcag tgatggtcag ctacctcatc
      121 gataactttg caacagtgcc cttcttgaag catcatgctt catgaagaaa aactggcacg
      181 tcatcaacgt aaacaggcga tgtatacccg aatggcagcc ttcccggcgg tgaaaacgtt
      241 cgaagagtat gacttcacgt tcgccaccgg agcaccgcag aagcagctcc agtcgttacg
      301 ctcactcagc ttcatagagc gcaatgaaaa catagtttta caggggacat ctgacatcac
      361 aaatccccgg gtggggattt gtgtataaga gacagcccat ggaggaaggg gatatatcgc
      421 agctctgacg actcactcct tatttacccg ggaaatactt ctccagcaat gcatacagga
      481 tggcagcggt ttcagcatcc attatgccgt cataattttt ctgccggaaa tgaagctgaa
      541 aagcgcggac aagcgcctta tatccgtcat ccgtctccac accagaaata ctgtatccat
      601 aggtcttcag ccgtgacacg atatcaacct tatccggaat gccctgcgat tcaaaaaata
      661 aatatttagt cagtaatttt atgtttttcc tgaaaaaatt actctttttc gaactccagt
      721 gagcgttcac gactcatcag tgtgaaaatg atggcaaaaa cagcagacag tatcagtacg
      781 ataaatgtgg ccccccatcc cagataatcg acaacaagtc ccattcccag tgtcgcaaca
      841 attgagccga agatatagga aaatgtgcct gacagcccta acagaataag tttacgcgta
      901 aatcgccctg aatccagaag agcattaagt atcacataaa caggttccat accagtcctg
      961 accgcatcag gacgccagta aataaaatct cgtagtccac cggcatcaga gacaagagta
     1021 atcgtcgtta ccggtatttc gactggtttg tactggtgtg agcggacaaa actactgtac
     1081 gtctctcata cacgctcgta agcggccctc acggcccgct aacgcggaga tacgccccga
     1141 ctgcgggtaa acccttgtcg ggaccactcc gaccgcgcac agaggcattt tcatcgctga
     1201 caggccgcat ggcctcgcag agccggaggg ggactggtga tattcggacg accgtaccgg
     1261 cttgcgccgg gcttcggcgg ttttacacct gtgctacatg taaccaccgt atgccgcctt
     1321 atatgcgctg actcgcatta tttgccttgt tccgtctgaa agtaatcact atgattaaat
     1381 atgattaaca actaatcggg tatgcaaatg aaaaacaata ccgcacaagc aacaaaagta
     1441 attaccgcgc atgtgccatt acctatggct gataaagtcg accagatggc cgccagactg
     1501 gaacgctccc ggggctgggt tatcaaacag gcgctttctg catggcttgc ccaagaggag
     1561 gagcgtaatc gcctgacgct ggaagccctg gacgatgtga catccggaca ggttatcgac
     1621 catcaggctg tacagtcctg ggcggacagc ctcagtactg accatctgtt accggtgcca
     1681 cgctgatgga actgaagtgg accagtaagg cactttctga tttggcgcgg ttatatgatt
     1741 ttctggtgct ggccagtaaa cctgctgccg ccagaacggt gcagtccctg acacaggcgc
     1801 cggtcattct gttaactcat ccacgtatgg gagaacagtt gtttcagttt gaacccaggg
     1861 aggtcagacg gatttttact ggcgagtacg aaatccgtta cgaacttact ggccagacta
     1921 tttatgtatt gcgcctgtgg cacacacgag agaacaggta gattgggtgt ggaggcaact
     1981 ttaacatagc ctgcaggtat taattctgga gcattgttat caggccagat attttgcatt
     2041 ccattaaata atcttgtagg agataaaata aattacgatg aaataacgaa aattaccgct
     2101 cgtgactggc ggcaatatcg cgctccgggc tggcaaataa cccatcagaa acgttattgc
     2161 caga
//
LOCUS       DAETQL010000246         2162 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.250, whole genome shotgun sequence.
ACCESSION   DAETQL010000246 DAETQL010000000
VERSION     DAETQL010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.250"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            80..259
                     /locus_tag="K0W65_004359"
     CDS             80..259
                     /locus_tag="K0W65_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097331.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            311..505
                     /locus_tag="K0W65_004360"
     CDS             311..505
                     /locus_tag="K0W65_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097332.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1304..1640)
                     /locus_tag="K0W65_004361"
                     /pseudo
     CDS             complement(1304..1640)
                     /locus_tag="K0W65_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          688 a          444 c          450 g          580 t
ORIGIN      
        1 atactggttg tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat
       61 tgctttacga gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact
      121 gcaacaccgt atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga
      181 cccattgcga tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa
      241 aacacgtcca ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga
      301 ggaaaaccgg atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca
      361 acgggagttt gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt
      421 tccacttgaa gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt
      481 cttagaatgc aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg
      541 gcatcgttat cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct
      601 ttgtttgccg gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg
      661 acattgtaat aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat
      721 cgttgacaat atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct
      781 gaagcatccg acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg
      841 gaactttgac aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg
      901 gaaaagtggt acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag
      961 cttgtcagaa aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag
     1021 tgcccggcca gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaatcgc
     1081 aggctatagg caaagttaaa gccccatgga gtagcacaaa atatgccgca caaaggaaac
     1141 ggtctgaata acgcagtgat gaagaacttc ttcagcacac tgctaaaacg catagtgatc
     1201 gagcgctgat tctggcgaac aactgaacta atacatcaga atctgcatta tgttaaataa
     1261 atatgaaaag atggtttaaa taccccgtta cttttgactt acactatacg gtatcgcatc
     1321 gtttaatatt cgcaccggcc agatttttat ttctattagt tgtcacaata ctgaatgcgt
     1381 acgaccacag tattctggct cctgtgtggt tatgctttaa ttctgcgttc cgggcagata
     1441 agcagttgct tgcaggaatc cttcttgtgt taatgtcagt tccccttttt accagtgctg
     1501 atttccacat tccgtccaac agagcttata gcctttccct ggattatagc attgtccggc
     1561 tgaagttctt tttgaataat aatagaagca ctgctggcag atccagtccg tttttcataa
     1621 cccactgtac tgataaccat aatctaatca gtagaaattg agtcgaaaat aagcactact
     1681 ctatacagga taattagagg tcagtttgat tattcacaat tcatcatcag cattttctat
     1741 ttctgacgaa atcaacatga aaataaccat atatgataat tattataata acggctttaa
     1801 ttggaataca tatattacaa cgtattatat ataattggta ttctgggaac tatattctca
     1861 aaatacagta gaaacgaggt atgtttctgg tggaaaggac agtgggatta aaaagtaaga
     1921 attgataaaa aaaacgccag caacacatag tgccggcgga gggaataccc catggagaaa
     1981 atgtgatgcc tagagcatca tgaatatata attaaaaata gttagcgttg tcaatacttt
     2041 cacaaaaata attttcgtag tatagaaaga tatttttatg catgacctac ctgaatttgc
     2101 tccgggtaga ggttataaat aaaaattgaa tcacgacaaa cacaatattc atagtatggc
     2161 ga
//
LOCUS       DAETQL010000247         2153 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.251, whole genome shotgun sequence.
ACCESSION   DAETQL010000247 DAETQL010000000
VERSION     DAETQL010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.251"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..769)
                     /gene="ipaH7.8"
                     /locus_tag="K0W65_004362"
     CDS             complement(<1..769)
                     /gene="ipaH7.8"
                     /locus_tag="K0W65_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HBI3097333.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1098..1277
                     /locus_tag="K0W65_004363"
                     /pseudo
     CDS             1098..1277
                     /locus_tag="K0W65_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1294..1984)
                     /locus_tag="K0W65_004364"
                     /pseudo
     CDS             complement(1294..1984)
                     /locus_tag="K0W65_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          630 a          398 c          494 g          631 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgcgagtcg
       61 acaatggatt ccctgaaata ttaatcctgt aacttgaatc taattgtgaa aaactctccg
      121 gaagagtggt tatctgatta aaatgaaaat aatattcctt ttgtctgata ttgtttctat
      181 cagaaaattg aggaaaagta accagattat tgcgctgagc acagagaatt tccagtttat
      241 ccggtaagtc gggtaatgtt gtcagcctgt tataataaac atgtaaaaat ttcaattttt
      301 cgggaagagc cggtaagtta tatagcctgt tattttcaac aaataatgat gttaaatggt
      361 ttggcaaaac aggaaggttt tccagttgat tagaacgaac acttaaagtt gttaatgact
      421 caggaagttc tggtatcaca gaaagcctgt tattatcagc attaagttct ttaagaaatg
      481 caggcagctt tggcaatgat gttaatttat tattttctac aatcaaagca gaaatatgct
      541 ttggtaattc gggaagagac gtcaaattta ggtctgataa atttaatact gcttcttgat
      601 tctgaaagca ctgggagagt ctgttaaaag caatgcctcg ctcttccccg ggagaagagt
      661 tcttttccca ttcagtcagg attctcagat aatgttcatt actggttgag tttgagttaa
      721 tagaggggga gcaagaaact gatgagtgtg tattatttac agagaacata atgctaggtc
      781 aaccttatat ttgtgagaat aaatatttaa aattgtatat ttatacaaat ttcacttacc
      841 atttccgata ttaagtttta atagtttcca ttttacacat aacagaagtt agttgtaatt
      901 tatttatgga ggttttctat ggaattcttt ttttattgtg gctgcagtca ataataacac
      961 tgaattcaac attccagtga gcaaggaaat gtaagccgag taagtatatt tgttttacat
     1021 catattttag tcaagctcat ttattcctat tatagaatga caaattaaaa ttactgtaaa
     1081 aaaacgatac acatttaata cgagggaggc cggcagaaaa acggcttgcg gtcaggcagg
     1141 agaaatgtgt gccgcgaatg aagtccttgt atgactggat aaagctccag aggaaaactt
     1201 tgtcaaacca tgctgaaatg gtaaaggcgt tcaactatat cctgaatcac tggaatgcac
     1261 tgaatgagtt ttgccgggta gtacttccaa tcattagaat atgtgctttt caatgaagga
     1321 accgatgact ttttcgtgga tttccacgga acgcgagaag cagattgttt tacgtgccag
     1381 ccgcttgatg cgggggcgga gggtcaggtt gttacgttca tacgttgcgt gaagattttg
     1441 ccggtcaggt gtttctcctc cggcacttct ctggcgtagc tgccccagtc atcgctggtc
     1501 accatgccga tacagaacgg agtcaacagg gctaacagtt cccgacaggt ctcatcattc
     1561 cgtggcccgc aagtgtaagc caacacaccg ccagttttag tgttgtacgc ataccagggc
     1621 cagtgttgtt gcgccttgct gccaacgaag cgccactgct catcaagttc acagataagg
     1681 gcaacatcag cgtgagcgac tggcgcagat gttatccgcc ggggcgagag ctttttaatg
     1741 tccgtatgac ggtattgatg ccgattttca gtgtcctggc ggtatcacgg ccccctgcgc
     1801 cgttatgtgc catttcaacg atctgctcct taacgccgag ttttctggct tcataagagt
     1861 aagtgagctg gaaaacacgt ttacatgagc gacaacggaa acgctcatgc cgggaacaac
     1921 tacggccatg tcgataaact tcatcggaat tacagcgcgg acaatgaaca gtaacggtag
     1981 tcatggaaaa cctcaaatac cagtagatta ccagaaactt ctatcgattg aagacattac
     2041 cttttgccgc aataggtggg gtaacattgg tgaaaacgca ttacgccggg tggcagttgg
     2101 aaaaaaatta tcttttttcg gctctgatcg tggtggagaa aatgcggcaa taa
//
LOCUS       DAETQL010000248         2135 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.252, whole genome shotgun sequence.
ACCESSION   DAETQL010000248 DAETQL010000000
VERSION     DAETQL010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.252"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..180)
                     /locus_tag="K0W65_004365"
     CDS             complement(<1..180)
                     /locus_tag="K0W65_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097334.1"
                     /translation="MRKLKMMLFGASLIMVVGCSSKENALCHPQPKPPAPPAWAMMPP
                     SNSLQLLDETFSVSGT"
     gene            complement(433..930)
                     /locus_tag="K0W65_004366"
     CDS             complement(433..930)
                     /locus_tag="K0W65_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBI3097335.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRD"
     gene            complement(996..1625)
                     /locus_tag="K0W65_004367"
     CDS             complement(996..1625)
                     /locus_tag="K0W65_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097336.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1629..1844)
                     /locus_tag="K0W65_004368"
     CDS             complement(1629..1844)
                     /locus_tag="K0W65_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBI3097337.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          547 a          568 c          470 g          550 t
ORIGIN      
        1 cgtcccggat accgagaacg tttcgtccag caactggaga gagttcgacg gtggcatcat
       61 tgcccatgcc ggaggcgctg gaggtttcgg ctgaggatgg cacagggcat tttcctttga
      121 cgagcacccg accaccatta tcaagcttgc gccgaagagc atcattttca gctttcgcat
      181 cagctaactc cttcgtgtat ttagcatcga gtgcatcagc atcacgctgg cgctgctgca
      241 tgtcagtaat ggttgcgttc gccagcttca gttctccggc gtttttgtcg cgctgctctt
      301 tgtaggtaat ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc
      361 agataaccag agcggagata atcgcggtga ctctgctcat tgatctatcc cccaacaggc
      421 taatgcgctt tcctagtcac gacgaataac ctgtccatag cagttatttg aacgtatgcg
      481 gcaatcgcgc ccaacatctt ttatccacca gcgaatcgcc tcgcatgcgc ccttacgatc
      541 accggcattc agccgcttat aaaacgtcga cgggaaacac ttaccggggc caatgttata
      601 ggggcaaaat gacgctatac ccgctttctg gggttcggtc agaggtactt taatattgcg
      661 ctccacccat gccagcgcct tatcacgttc aatagcgtta acctggtcac atttttcctt
      721 cgacagcttc attcccggta tgacaggctt accatccacc attgtggcac cacgacagat
      781 ggtccatatg ccagaaccat cacggtatgc cgttgtgtga ttaccctctt tttcgttcag
      841 aaactggtca agtatctggg gagcagacgc gcctgcggca atcagcgcca gaacagcagc
      901 cgacaggccg tatttgattt ttgcgctcat ggatatttat caggatttat cggcaacaaa
      961 taacgagcca acttatatac gccttttaag ataagtcagc cccggctgga tccagtcatc
     1021 aggctctttc ttaaaggggg agtattgaaa tcacgaagaa gagcctcccg caccgcagca
     1081 tccatgtctg caccactggc aagtgcttca atctctgccg ccacctgcaa ataccccatg
     1141 caacggccaa tgcgtttcat aactctggtg ttttcgtctt tcaggcatct gatagcgaga
     1201 ttaataattt tcaggcagtg atgatgcgat gcccggacag gttttccgtt ctcataaacg
     1261 acgaactcac cctcttcaca ggaaaccctg aaaggccgat ggggttcctg gtctttatcc
     1321 ggtgcagacg caataaaatc acttttatgc tcttcctctc cggcactgtt aaccgtaatg
     1381 acgtactcag gccgcaggta agccgcctct ttttctgaca atgcggccgg taaaaacttt
     1441 tcctgcaaaa acttcataaa agtctcgtga gcgactttgt ggcagtaatc gtaaatcgcc
     1501 gcataatgct catcgcggcg tttgttttca tcttcagaac tcaccaacgc cgacaatttt
     1561 ttattaagct cagtgatttc attttccagg tgattgaatc gctgattcat ttcttcatgc
     1621 ttcattattt actctccccg tgcagcctta cgcttgtctt ctcttatttt gaaatacaga
     1681 ttcgtcagat aagtcagaag ccccagaagc agacttccca gcacaccaat cgcagcccac
     1741 tgtgatggcc tgacctgatc caaccactgc aaaaaccagt agccggcact gccggcggag
     1801 gtgccgtagg caatgcccgt tgaaattttg tccatggatt tcatagcctc acctccgcaa
     1861 ataacggatg gcgtagtttt acactgagaa atgaaaggga tttgaaaaga aaaaaacgca
     1921 aaagcgggcg aaacgatata tacagtaagg aaagcactct atccaacaaa ccacccacag
     1981 ttaatcggaa taaaagcaga gtgcttatga atgatcgcac gcccgaaggt tagtatttct
     2041 gcacagcaat tttgcaaaaa aaggcgatca ttcataactt aaacgtcttt cagtcactcc
     2101 gggatttccc atcatcgcag actgaaagac tctga
//
LOCUS       DAETQL010000249         2123 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.253, whole genome shotgun sequence.
ACCESSION   DAETQL010000249 DAETQL010000000
VERSION     DAETQL010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.253"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..124
                     /locus_tag="K0W65_004369"
     CDS             <1..124
                     /locus_tag="K0W65_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3097338.1"
                     /translation="CFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            complement(273..1727)
                     /gene="ospC2"
                     /locus_tag="K0W65_004370"
     CDS             complement(273..1727)
                     /gene="ospC2"
                     /locus_tag="K0W65_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HBI3097339.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          661 a          373 c          355 g          734 t
ORIGIN      
        1 gtgctttggg cggctgaaag aatatcgccg catcgccacg cgttacgaca aaacggcgag
       61 aaattacctg gcgatggtga aactgggctg catccgactc ttttatcaac gcttacgtaa
      121 ttaagggaca cagcctagtc tccaggattc ccggggcgtt caccacatgc aatgtgttgt
      181 tgcaggggaa cggtctgcac catcccctat atcgatcaat ataacaaaaa tgacaacgtc
      241 agattcaggg cacaacctaa atatatttta ggtcacctcc caaatcgttt gcctgatacc
      301 gctccatttt ttaataaaaa atcaattgtt tttgaatctt tgcttttcat tgcattgtca
      361 agcatagtgt ccccactatt tgctttttga aatcttttgt ttacatcaac caatccatta
      421 ctaataaaat attctagcac tttatagttg gcactatctt cactaagtaa atactcaaca
      481 tcatacagct cacaatacat attgttgttc attttctcca tctctgcgat atcttgtttt
      541 gtaaaattaa atttagatag taaataaagt gccatatcag attttgcatt gcctagagaa
      601 taagcaagag cttgatgagc cattttttta tcagtcacct tattgttaat ttcttcataa
      661 ttggatgctt taatggcatc ctccaaagat atatctctga gtcttatctt tttgaagtta
      721 tctgtgctta gcattcttgg tatatgatac tcaagctgaa acacaaagtt tataattcta
      781 tccagactaa caggatcaat gtttttatta taacaaaact ctcggaatct ttgatcttta
      841 ctctttctta taaaatcaat aagatgcaac ccaattccaa gcctcatatc tttagtatta
      901 aatattggca catcagtttt tccctgatta ccatgcacat acctgctaac ttcatccaca
      961 acctctttaa agttatctaa gaaaaatgat tggtgctcat ggtaaaaaac aggtggaata
     1021 gcattatcaa agtggtcggt taatgtcata tatccatatg gagagtcatg atcaatgata
     1081 tacgcattag caccaaaatc cacagtatga tgtgttttat ttagtgggag tgtttcttga
     1141 tgattggaaa tttcaacccc aaaaaaaaca aaatcgtggt tagctatttc ttcaatatcc
     1201 tcagagaatg tgtttttata tgtttgtata ttctcagact taattttatc aaaggactta
     1261 atattaagag tattatttga caggttagcg ttactttgat gtctgaagtt aaaatcagaa
     1321 ttaataataa tagagagcaa gtgacgttca tccgaagaaa gaggatgagt tttttgtatt
     1381 atttcagaaa gatagtgtct ggacacctta tcgaggtgct caagtgaccg aaatgatgac
     1441 cgtgctccag atggcgcttc aatagaaaga tttaatgatt tgaaactggt cccttgagag
     1501 aacattttac tataactttg cgctgctata gacttcctta aaaagctttt cacagtatca
     1561 gcacagtgat ctaaatgttt ttcacttatg ccagaagagg atgagtttgt tacgcatgat
     1621 atgttttttt gtaatgcttt tgtatctttg ttcggattta tttttccatc aacaagatca
     1681 atattgttct gaacattaat atgatttact gcttcaggta ttttcataaa acatccccca
     1741 ttatgtgcgt ttttatctgc agttatttag aataatatta tctaacttaa tattttcttg
     1801 tatgtattat aacgaacata ttttaatatg ttgtacgatt ttcactctga aatcccctga
     1861 gtttaatcac acttccttta ctgaaaggaa ttattgcaac agtattattt gtttttggat
     1921 atatatcttg cgccttgtta tgtagttaat gctcagaata tgatagataa aatagagacg
     1981 caaaacaggg catgctcata acatgatgat aacataaaaa attagtaaca ataaatgaaa
     2041 gaatcatggg cgctcaacaa aaagagggta atgctgccaa cttactgatt tagtgtatga
     2101 tggtgatttt aaggtgcttg cgt
//
LOCUS       DAETQL010000250         2055 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.254, whole genome shotgun sequence.
ACCESSION   DAETQL010000250 DAETQL010000000
VERSION     DAETQL010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2055
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.254"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(38..583)
                     /locus_tag="K0W65_004371"
                     /pseudo
     CDS             complement(38..583)
                     /locus_tag="K0W65_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1245..>2055)
                     /locus_tag="K0W65_004372"
     CDS             complement(1245..>2055)
                     /locus_tag="K0W65_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097340.1"
                     /translation="NIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVTA
                     PSGTEITVEPIEQAILVSHQTKLWDQKSSTGFTQGTLRQDALNICDNVVRTINSKMVD
                     VLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVTDYGIIAHESHLKAL
                     SRLAAKQGFGGEDAIVDMLGTDIAYYNGVDRGVFMMAKRFTALSFGCFRHDGENITVV
                     LSRDGNTQSHDLEILGKVFVVAEAATTIKMGTGSAAAVLPVVKRLSFTKTS"
BASE COUNT          558 a          463 c          495 g          539 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcagaat tccatccagt cgttttgggt
       61 tgctaatgat gtcctgcatc cgcttaatct ggtcgggcgt agacatataa ccccacaggg
      121 tgagcagaac cttttttgcg gctacactat ttccttggtc tgatactaat gccatatagc
      181 cccataaact atccctcagc cagacagcat catagtgggt ttctgccgct gttttgtcat
      241 cttcgaaagt agaagcaaga ataaacgttc cccagggttt ctccgtcaac gttaactttc
      301 gtgtgttttc tgttaatgtt agcaatcctt gagtttcgtc tttggtatag tgactcttta
      361 tttcattttt taatttggct gagtgaatca gaagacgata attaggatac ttatctggat
      421 ttaacgataa ataactttta tctgttgctg cccccctgga attaagaatg ttaaatacag
      481 attcgcttgt cacttctgcc tctgtcgcct ggcatgtgcc gcacgaaaat ataccagcga
      541 gcagaagagt taatgcagat ttccctttca taaaataatc catcaatcgt ttgttaaaag
      601 cgtagataac cacttattca acaacttgaa aataaggtat tttacctgag ttgttacaaa
      661 aggattgcat tgcgtaaacg ctttttattt acaaaaaaat ggggaagtgt tacagcgatt
      721 gcgaaaagcc tgaagtgatt gaattaagat aatatagaaa atcagataca gcaaaaatgg
      781 cagattgatc aggtgaagat aaattggagc gggcgaaggg agccgaggac aactagctaa
      841 cttcttgttt cttaaagact gatgaatcca gtggttacag tgatggttac acatcgagag
      901 gctgacggtc aagacttgaa gcacgtaata ttcccataaa aggaatagaa cacagtcact
      961 gactacccgg ccctttggtc tgactgaggg tcgggctatg gggtaaatcg tcaggcttat
     1021 tgtattagat ggtctctcac atttttcagt caaaaactca tcgtacttta ggccagacca
     1081 tcagcacgac taccagagct agctttagca ggaagaccac gagatagact ttgtgtaggt
     1141 ccattaggtc ctcctctttc cgtacccatg aactagattg ctagtgcaaa ataaaggcca
     1201 gacatccagt tacggaagtc tgaccaataa gtcgttatcg ttagttagct tgtcttagta
     1261 aagctcaggc gtttaactac aggcaggaca gcagcagcgc taccagtccc catcttgatt
     1321 gtcgttgcag cctcagcgac cacgaagacc ttaccgagaa tctcaaggtc gtgagactgg
     1381 gtgttgccat ctcgggacag gaccacagta atgttctcgc catcgtgacg gaagcaaccg
     1441 aagctcagag ccgtgaagcg cttagccatc atgaatacac cacggtctac accgttgtag
     1501 taggcgatgt ctgtccccag catatcgacg atagcgtctt caccaccgaa gccctgctta
     1561 gcagcaagac gggagagagc cttgaggtgt gactcatggg cgatgatacc gtaatcagta
     1621 acctcagaac cgaaagaaga ctcggtgttc tcgtacaggg catccatgat agcgtctgct
     1681 ttggccgtca ggctcccggt caggacagga agctcgacgg tcttcagcag cttagaggac
     1741 tccagaacgt caaccatttt agagttaatg gtacggacca cgttatcgca gatgttcaga
     1801 gcgtcctgcc ggagcgtccc ctgagtgaaa ccagtagagg acttctggtc ccataacttg
     1861 gtctggtggg acacgaggat agcttgctca atcggctcga cggtgatttc agtaccagac
     1921 ggagcggtca cgacttcagg tcgcttacca ccacggacag gaacgaccac agagcggtca
     1981 gccagagtgt gagcaattat tggcgtcggt aagctgttac gggtcggcag gacttctaca
     2041 gggaacggaa tgttt
//
LOCUS       DAETQL010000251         2050 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.255, whole genome shotgun sequence.
ACCESSION   DAETQL010000251 DAETQL010000000
VERSION     DAETQL010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.255"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..2050
                     /gene="sat"
                     /locus_tag="K0W65_004373"
                     /pseudo
     CDS             1..2050
                     /gene="sat"
                     /locus_tag="K0W65_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          750 a          361 c          405 g          534 t
ORIGIN      
        1 ggggccagtc tactcatacg gcatgatgat ttataaaaat gataaaacat ttcgtaatct
       61 tgaaatattc ggagacagcg gctctggagc atacttatat gataacaaac tagaaaaatg
      121 ggtattagtc ggaacaaccc atggtattgc cagcgttaat ggtgaccaac tgacatggat
      181 aacaaaatac aatgataaac tggttagtga gttaaaagat acctatagtc ataaaataaa
      241 tctgaatggc aataatgtaa ccattaaaaa cacagatata acattacacc aaaacaatgc
      301 agataccact ggtactcaag aaaaaataac taaagacaaa gatattgtgt tcacaaatgg
      361 gggaaatgtt ctgtttaagg ataatttgga ttttggttgc ggtggtatta tctttgacga
      421 aggccatgaa tataacataa acggtcagag atttacattt aaaggagcag gaattgatat
      481 cggaaaagaa agcattgtaa actggaatgc attgtattcc agtgatgatg ttttacacaa
      541 aataggcccc ggtactctga atgttcaaaa aaaacagggg gcaaatataa agataggtga
      601 aggaaatgtt attcttaatg aagaaggaac atttaacaat atataccttg caagcggaaa
      661 tggtaaggta atactaaata aagataattc ccttggcaat gatcaatatg cggggatatt
      721 ttttactaaa cgtggtggca cgctagattt aaatggacac aatcagactt ttactagaat
      781 tgccgccact gacgatggaa caacaataac taactcagat acaaagaaag aagccgttct
      841 ggcaatcaat aacgaagact cctacatata tcatgggaac ataaatggca atataaaact
      901 aacgcacaat attaattctc aggataagaa aactaatgca aaattaattc tggatggtag
      961 tgtcaacaca aaaaatgatg ttgaagtcag taatgccagt cttaccatgt aaggccatgc
     1021 aacagagcat gcaatattca gaagcacagc gagtcattgc tccctggtat ttctttgtgg
     1081 aacggactgg gtcaccgttt tgaaagaaac agagagttca tataataaaa agttcaattc
     1141 tgattacaaa agtaataatc agcagacctc atttgatcag cctgactgga aaaccggggt
     1201 gtttaaattt gatacattac acctgaacaa tgctgacttt tcaatatcac gcaatgccaa
     1261 tgttgaagga aatatatcag caaataaatc agctatcaca atcggcgata aaaatgctta
     1321 cattgataat cttgcaggga aaaatattac taataatggt tttgacttca aacaaactat
     1381 cagtactaat ctatccatag gagaaactaa atttacaggt ggcatcactg cacataacag
     1441 ccaaatagcc ataggtgatc aagctgtagt tacacttaat ggtgcaacct ttctgaataa
     1501 tactcctata agtatagata aaggagcaaa agttatagca caaaattcca tgttcacaac
     1561 aaaaggtatt gatatctccg gtgaactgac tatgatggga atccctgaac agaatagtaa
     1621 aactgtaacg ccgggtctcc actacgctgc tgatggattc aggctgagtg gtggaaatgc
     1681 aaatttcatt gccagaaata tggcatctgt caccggaaat atttatgctg atgatgcagc
     1741 aaccattact ctgggacagc ctgaaactga aacaccgact atatcgtctg cttatcaggc
     1801 atgggcagag actcttttgt atggctttga taccgcctat cgaggcgcaa taacagcccc
     1861 caaagctaca gttagcatga ataatgcgat ctggcatcta aatagccagt catcaattaa
     1921 tcgtctagaa acaaaagaca gtatggtgcg ttttactggt gacaatggga agtttacaac
     1981 ccttacagtg gacaacctta ctatagatga cagtgcattt gtgctgcgtg caaatctggc
     2041 ccaagcagat
//
LOCUS       DAETQL010000252         2035 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.256, whole genome shotgun sequence.
ACCESSION   DAETQL010000252 DAETQL010000000
VERSION     DAETQL010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.256"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..362)
                     /locus_tag="K0W65_004374"
     CDS             complement(<1..362)
                     /locus_tag="K0W65_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3097341.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGL"
     gene            complement(429..1643)
                     /locus_tag="K0W65_004375"
     CDS             complement(429..1643)
                     /locus_tag="K0W65_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HBI3097342.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          633 a          493 c          414 g          495 t
ORIGIN      
        1 agaccgatac gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc
       61 attaatgacc agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc
      121 aaaataccgg aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca
      181 ccaaactctg tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat
      241 gacgaacggt cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc
      301 tgaatacgac gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc
      361 atttttagct ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg
      421 aaccgttatt accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc
      481 gggtcatacg gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca
      541 tcgcctgggt gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt
      601 tgttcggaat agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc
      661 agcgccgccg catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc
      721 tgataaagcg atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg
      781 atcgtcgtgc gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata
      841 ctgttaaaca cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa
      901 cattcctggt tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg
      961 ctcttcccaa agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga
     1021 tgttcgttaa aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga
     1081 tacttttctg aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac
     1141 gccagtcctt tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc
     1201 acacgaatcg ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca
     1261 atcgtacgcc cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc
     1321 agttgataca atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg
     1381 gcagaaatgg ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat
     1441 tgccagatat cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct
     1501 ttcagataag aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa
     1561 agtccgcttt cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa
     1621 acatctgcag caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa
     1681 cctaatttta cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca
     1741 ttaaaaacaa aaatttctac cgaggagaaa aaataccctc aaaaaccacc tgaataatta
     1801 accattattg ttaaaacaac acgttgaata atcacataac aaactccatt aatcattgaa
     1861 aatgagcaca gaaatagaaa ttaattatat tcaattaatt aaaatgtgta ttgcttgatg
     1921 tatttaaaaa tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag
     1981 gaatgtgaca aataatggtt ctggttgggc gttaaattta aggtcaccag aatgc
//
LOCUS       DAETQL010000253         2029 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.257, whole genome shotgun sequence.
ACCESSION   DAETQL010000253 DAETQL010000000
VERSION     DAETQL010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.257"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1824)
                     /locus_tag="K0W65_004376"
     CDS             complement(<1..1824)
                     /locus_tag="K0W65_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HBI3097343.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2029)
                     /locus_tag="K0W65_004377"
     CDS             complement(1932..>2029)
                     /locus_tag="K0W65_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3097344.1"
                     /translation="WNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          485 a          579 c          430 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagc
//
LOCUS       DAETQL010000254         1977 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.258, whole genome shotgun sequence.
ACCESSION   DAETQL010000254 DAETQL010000000
VERSION     DAETQL010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.258"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(102..461)
                     /locus_tag="K0W65_004378"
                     /pseudo
     CDS             complement(102..461)
                     /locus_tag="K0W65_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(461..1381)
                     /locus_tag="K0W65_004379"
     CDS             complement(461..1381)
                     /locus_tag="K0W65_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBI3097345.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1519..>1977
                     /gene="pgrR"
                     /locus_tag="K0W65_004380"
     CDS             1519..>1977
                     /gene="pgrR"
                     /locus_tag="K0W65_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBI3097346.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVLRCTGNSG"
BASE COUNT          489 a          487 c          464 g          537 t
ORIGIN      
        1 gcttatcgcc ctggcgagac gacgctgcga cgttctgttc gccatgatgc gcgacgggac
       61 tttttatacc ccgcaggggt cataacatgc ttgacaactt aataggggca ccccccacac
      121 acacccagca cgccaatgcg ttcagcatca acatatggca gagttactgc ataatcgata
      181 acacggctaa tatcctctac gcgctgtgtc ggatcttcta tccaacgagg ctacccacca
      241 gattctccct ggaaactggc gtcataagcg agaacgacat agcctttttc agccaatgcc
      301 ttgccataga tattgccaga cgtctgctcc ttacaactgc caaaaggatg gacgctgata
      361 atcgccggat actgttttga ctcatcaaaa tttggtggaa actgaatatt ggcagcaata
      421 tgccaggcca tatcactatt gcggatttga atatgtttca ttacagagtt tcctcaaaga
      481 acggagctaa tactgaaatt gcttcagcca cataggcttt gccgtcataa agatccatat
      541 ggtttgcgcc ttcaacaatg tgatagcgtt tatcctggct tgatgcccga tcgtacaggt
      601 cgtcactcat ccatttgctc cctgcctggc tgcccgccac aatctgcatc ggctgagtca
      661 ggtacacttc cgccatatgg taagcatcat aggtaataat ctggttaagg ctgcgcaaag
      721 tagcgtaacc cggtgctgtt ggatactgcg cacgaggtgt gtggtaatac tcccaggcct
      781 gacgcagttc ttcgttcggc gcatcggcct ccttcattgg agccagtggc ataatggcgt
      841 attcttcgct gctaatatcg ctggttcggg cgtttgagcc agcttcaatg tgtggcagag
      901 catcaataga tttcacattg ttttcccaac cattgcggaa catcgaacca atattgaccg
      961 cactaacggt accgatggcc ttgatgcggc gatcctgaat tgcagcattg gctgtatatc
     1021 ctgcaccggc acaaattccc atcgcaccaa ttcgggtatt gtcgacataa gaaagcgttg
     1081 tcaggtaatc aatcacggca ctgacgtctt cagtacgaat gtatgggttt tctaactgac
     1141 gcggctcgcc gccactttca ccttgataag atgcgtcata agcaatagtg acaaatccct
     1201 tttccgccag cttttcggca taggttccgg ccgtttgctc tttaacgccc ccacctgggt
     1261 gagataccac aattgccgga tactgacggg tttcatcaaa ttttggaggg aaatagatca
     1321 ctgcagacaa agagatggtt ggattattgc tgttagtgaa gctgacttta ttattcatca
     1381 ttcagttcct catggagttg tcggttcgtt ttgacggttg gtgatatcac tatagatatt
     1441 gatcattaag ttgattagac cctaaatcat gattagacct atcatttaaa tgattaatag
     1501 attgtatgga gatactgaat gaagcgtgaa gaaatcgctg atctgatggc gtttgtcgtc
     1561 gttgcagagg agcgtagctt cactcgcgca gcagcccgcc tgagcatggt gcagtcagct
     1621 ttaagccaga tagtgcgccg tatagaagaa cgattgggat tgcggcttct gacgcgaacc
     1681 acgcgcagcg ttgtacccac tgaagcgggc gagcatcttt tgtctgttct tggccctata
     1741 ttgcatgaca tagattctgc cctgacatcc ctgagcgatc tgcagaaccg cccatccggg
     1801 acaatacgta ttactactgt agaacatgca gcaaaaacga tattgttacc agccatgcgc
     1861 acattcctga aatcgcatcc tgaaattgat attcagctta ccattgatta tggtttgacc
     1921 gatgtcgttt ctgaacgttt tgatgcaggc gtcctgaggt gtactggcaa tagcgga
//
LOCUS       DAETQL010000255         1976 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.259, whole genome shotgun sequence.
ACCESSION   DAETQL010000255 DAETQL010000000
VERSION     DAETQL010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.259"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..837)
                     /locus_tag="K0W65_004381"
                     /pseudo
     CDS             complement(1..837)
                     /locus_tag="K0W65_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          533 a          465 c          456 g          522 t
ORIGIN      
        1 agctgctcta cggtcatatt tttaattgcg ctccggttta caagagccca cccctgtttt
       61 tccagataaa actggaaagt atccagggta cagaccagtg caccgtcagg aacagtttcg
      121 gtgaatctga tattgccgtg ttcgtcgaag tgcacaacca gagtgcgacc gttacccgga
      181 atcatcttgt cagcggacgg ggtattattc tggtgcagtt ctgcctccat gcggtcgaac
      241 atcagcgatg taggcctctt tgaatgcagc ggcttttttc ccagtgaagc ccatcaccag
      301 gaaaacgaag ccgtttttgg tgatttggta cattgggcgt ttttcgcctt tggtgtcggt
      361 gtaggtgacg tcctcaaaat tgagggcgtt aaattcaggt gagcattcag tatttgcgat
      421 ggcacgtaac acattgtcgt gtcgtttgtg gaagaactct gcaaccgcaa cagacgtagt
      481 gacagcgcga ccgttttcga tggttacgtc agggtgagaa agggcaggga tagtagccat
      541 gatggcatcc tcgagtgata agttaattaa ctcaccaccg aggttttcca cgaccataag
      601 ggtggtgaga cgtacagggg tggaaatacc ggtcactcga gaacccggcc agtcttgcga
      661 ctgccctgca catcccacca taatttgaat ggagctgtgc attacgcata aaaaaaccgc
      721 ttcagcgcgg ctatgcgctc gagtaacgtt cgggtttcca cgcccggccc ccgttttatg
      781 aggtgcagat gcactataat tccacccgtt ctggttttca atagctacat tcaacatttt
      841 ctcttacctt tcatcaccga agtgaacttt gttgatgcgg tgcctggtgc ctccaggtga
      901 cgttaaccag ttaacaatta acgccggata cagggaaacc cataaccacc cgatacgtgg
      961 gaactaatcg ctttttaact gttccgcgtg cgctcagccg cattcaccgc atcacaaaat
     1021 tcactttaaa aagggcggac atcagccagc aatgaaactg atgccgccaa aggtaccaat
     1081 caacatggag tgttgtggcg tggttgtcac ttaagcgtat ggtcaacctg acaacccggt
     1141 gtcctcaacg gggaaggaat aactccgcca tacttaccgc cgcgtcattt cgcgttgtgt
     1201 gcctgctttt aactacgtca ggcgaggtgg ttcctgttat tccccaacaa caagaaattt
     1261 gtataatctg aataccccaa caataagaag agtgagcaag tgatgtatta gcgatgtcat
     1321 ctctcaaaga gactgcatca atgttaaaat tattcaatga agcaaaaact caggctgagg
     1381 ttgatcgcgc tgtgtctgaa ataaactcta agttgtcaag cattcagctt gaatatttgt
     1441 catctcattg atgagctgac aatctctaaa agcaatgagg ctgcgctcaa agcaaaactc
     1501 acggagatag aaaagtttaa gttccaaagt gaagactgcg aacctcgcta attgattact
     1561 ggaacgattg tgtatgccag acagtgcaat actagctctg gcaagattac catgtatttt
     1621 tgtccagaat gttttgcgaa gagagcaata tctatattgc aacctttccc tatcgaggaa
     1681 ggcgataaat atacgaaaac tcaatgcccc tcctgtaaaa atgagtatct catggactac
     1741 aatttcaata atgatttttg agtcacggta ttcagatttt taaagaacat gccggatact
     1801 cacccgtgtc cggcgcacgc actccacctc acccgtggag aactccttaa ttaccaacct
     1861 tggcttcgtt ggttagccat taacgctggt atgtaatcat tctggcaatg cttaatttga
     1921 agtggcacac tgaatttggc cacctgaaca gaggtgatat gctcacctca gaacaa
//
LOCUS       DAETQL010000256         1969 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.260, whole genome shotgun sequence.
ACCESSION   DAETQL010000256 DAETQL010000000
VERSION     DAETQL010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1969
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.260"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            240..509
                     /locus_tag="K0W65_004382"
                     /pseudo
     CDS             240..509
                     /locus_tag="K0W65_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..732
                     /locus_tag="K0W65_004383"
                     /pseudo
     CDS             506..732
                     /locus_tag="K0W65_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="K0W65_004384"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="K0W65_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HBI3097347.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1690..1969
                     /locus_tag="K0W65_004385"
                     /pseudo
     CDS             1690..1969
                     /locus_tag="K0W65_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          560 a          432 c          462 g          515 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
     1621 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
     1681 cactatcgta tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa
     1741 catttaccac gggttgtggc cggatgtcgc ctgggcgtac caaaatcaac tgtctgtggt
     1801 atgttcgtgc gcttccgcaa cgctggcctt tcatggcctc tgcccgcagg tatgtcggag
     1861 caggaacttg atgcccttct ttacggaagt gcctccacag tacctgtcgt acttactgaa
     1921 agcaccgtta tgcccaaact cccggtggta aaaaaacgtc cccggcggc
//
LOCUS       DAETQL010000257         1919 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.261, whole genome shotgun sequence.
ACCESSION   DAETQL010000257 DAETQL010000000
VERSION     DAETQL010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.261"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(59..1708)
                     /locus_tag="K0W65_004386"
     CDS             complement(59..1708)
                     /locus_tag="K0W65_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBI3097348.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          452 a          510 c          491 g          466 t
ORIGIN      
        1 cctcctgaag aggataattt tacagccagc aggaagttag cggctggctg taaaaatatt
       61 atggttcgcc gtacaaacca attaaacggc gtaccacacc gttagtccag ccaaacccat
      121 cctgcaacgg atactcgcca ccgccgcctt cgcggggaac accatcggca atatggtatt
      181 tctcgatcat tttgtgctgt tccagataga actgattcac cgttttcagc cagctgcgcg
      241 cgatttcatc acccagaagg tcatcgccat acattttaaa tccctgaatt gccatccatt
      301 gtaacggtgc ccagccgttg ggtttatccc actgttcacc ggtttcgtac tcgcttgcca
      361 aaatcccgcc aggtgtcagt aaccggctgc gtacggcgtt tgccagacga tcggcctgtt
      421 catggttcgc catgccgaca tagagcggca caatggcggc agcggaaaac agcgccagtt
      481 gttcgcgtcg ccagtcgtaa tcgcggtaga tgccgttttc atcatcccag aggtaacggt
      541 ttaccgcatc gcgacgggca ctggccttct ggcggaacag cgcttctgtc tctttctcgc
      601 ctttcagcgc cgagatgttg gcgatggcgc tctccagttt aaacaggaag gcattcagat
      661 cgatggggat gaactgggtg gtacgaatgc tcgccagacg accagtatca cgcagccaac
      721 gggaagagta atcccaaccg gaggccgctc ccgcgcgtaa atcgcggtac acctcgttgg
      781 gcgggcgacc agaatgtttc gcggtttcaa cgtcctcaag ccaggattcg tcacgcggcg
      841 tgtcgcgatc gtcccagtaa cggttgagca gcgatccgtc cggcatccgc acaacatggc
      901 gataggcctg attagggatc aacgattctg caccgtccat ccagaaggca tattccattt
      961 taaggtggtc gagatagcgg cgcgcaccgc gtacaccatc ttcttcaaac aactccacca
     1021 tcagcgcaaa aaccggtggt tgcgagcggc tcaaataata ggtgcggttg ccgttgggga
     1081 tgtgaccgta gttttcgatc atccaggcga agttatcggc catgcatttc agcaaatctt
     1141 cccgaccact ttccgccagt cccagcatgg tgaaatagga atcccaatag taggtttcgc
     1201 taaaacggcc gcccgggaca atatatgact gcggtagcgc cagcagagaa gaccacggaa
     1261 tgtgatcctg tggttcgcgg gttagcaccg gccacagctg gtcgatatgc tctttcaggg
     1321 aattttgcgg gtccgataca tactcgctgg agtagacctc cggcagccag aagtgatttt
     1381 caacaaactt gcgcaagtca aaatcacgat gacggcgcac tttacggtag cggattaaaa
     1441 tatccagcgg gtccattttg ggtgcgcagt cgggaaaggt tttactgtcg gggaaaattt
     1501 tcgccgactg aacatgctcg aacagttcga gatagcgatc ggcaggcgtc agcgcatcag
     1561 aggcgggcag cccttcaatc atctcgggtt ccggttctgc ctcgatcatc tcatccagtt
     1621 ttaattcata cgggtccagt tcatagcaga gatcgacctc gatcatcagt tcgtctggat
     1681 tagggttttg aattttctga ttgagcataa ggatccaacc tccgaaattc gtcgctaatt
     1741 atcgggcttt tctttaaggg tagtcattcg ctccgcctgg tgggcgacaa agcgtgcggt
     1801 ggatcacgtt tattaacttg tcaaaaaata taacgcacta atatcaatgg aatttataaa
     1861 aatacggatt tggtgttgtt tttcattttc agaacattca atcctgctct cgccgcaat
//
LOCUS       DAETQL010000258         1861 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.262, whole genome shotgun sequence.
ACCESSION   DAETQL010000258 DAETQL010000000
VERSION     DAETQL010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.262"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..259)
                     /locus_tag="K0W65_004387"
     CDS             complement(<1..259)
                     /locus_tag="K0W65_004387"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097349.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARC"
     gene            complement(1012..1485)
                     /locus_tag="K0W65_004388"
                     /pseudo
     CDS             complement(1012..1485)
                     /locus_tag="K0W65_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1413..1779)
                     /locus_tag="K0W65_004389"
                     /pseudo
     CDS             complement(1413..1779)
                     /locus_tag="K0W65_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          426 a          519 c          442 g          474 t
ORIGIN      
        1 tgcagcgggc aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt
       61 ttttgtttcc ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat
      121 cgtcaaaatt atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt
      181 gaagttttac gacatagcgg aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg
      241 gtttagctga acgggccata taactacctg aaagacaatg atattgtctg tttttataac
      301 ggtaatcaca ggccatgaca agccgcagcc gtcaggccgc ctactcgggg cggtttagaa
      361 cgcttattta gtaccagatc ttacttataa taaaggggct tggcagtttg gataaactat
      421 tgtccttatt ccactaatac aaactttttg taaagtatat taaatatact tcatttcata
      481 ctgaaatccc cggaagtatt caatcaaaca attacaccat aaaaggaaga gggggcccca
      541 attaagttgt caagcatgtt tactgcgcaa tcgcttggtg ctccgtccct gtaccccgca
      601 ttggtactct ggcgctcatg gggcttccac ttgcgcttct cccttcacat cggggtgaca
      661 ggtccacgtt ccccacaaga gcctgaatcg acttcacgcc gcttttatgc cggatgccgc
      721 gcaggcagta aacatatccc acaggaacga ctccaccatt gttttgacat catctatatg
      781 ctttcgacac atcatcagcg gttcactttc gttcgtctct tcgaaccata cctgatcggc
      841 tcacgtccga ccttttccca tctcgctcac caccacatct cttaaatgca gcagcagtgg
      901 atggtttggt gcctgcgcct gcacgccgac accaaggggc cttccctcat ctcttgtgct
      961 gcatggctgc acagggactc acgtccccag gacgcgcctt cgtggcacac agtcatcggc
     1021 atagcaacag taagcaacgg caggtttcca ttgccagttt tctttgacag cagtacttcg
     1081 gcctcgctgg atactgtgat tccagtacca gcgatccttt ctggcttttc cactcaggta
     1141 gcgtttattc agatactggt cgaactcatt cagtatgatg ttcgacagca acggcgagat
     1201 cacaccgccc tgtggcatac tttcactgac cgcccggaag agaccaacat caacacggcg
     1261 ttttccacag cagtctcata aaacgtgtat cactgatcct gcggcagacg gctttcatca
     1321 gcagccggtg atgtactgtg tcgaagtagc tggacaggtc accttcaata acccggcgcc
     1381 ccgtgtttcc ccgcagtcgg tgagctgtaa tagtacgtga tgtctcccgc ccacttctgg
     1441 ttcgggccac tggcgtaaaa atcctgcgtc agcagatttt ctgatacagg caggccgtgt
     1501 gcacggtaac tgatcgggct gaacttctgg gaggcttttg cgctcagttc ctgacggcgc
     1561 agactggctg ccacggtttt tacgttaaag tggtaaccag agcacacagt tcatccgtca
     1621 ggcgtggggc accgtaacgc tggactgggt aaaagccttg cggacaacgc tgtcgcagtg
     1681 ctgacggaac tgcggacgtg tgtttatcct tgtccgccgc tgacaccacg tataccagcc
     1741 gctgcgggcc acccggagta cgcggcacat tgccttgatg acggtttacg cggtcgcctg
     1801 cggcaacggc tttccggctg acggtagtgt ggaccttttt accccggaga acaccggcaa
     1861 g
//
LOCUS       DAETQL010000259         1834 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.263, whole genome shotgun sequence.
ACCESSION   DAETQL010000259 DAETQL010000000
VERSION     DAETQL010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.263"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..182)
                     /locus_tag="K0W65_004390"
     CDS             complement(<1..182)
                     /locus_tag="K0W65_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBI3097350.1"
                     /translation="MAGCVGEPKGSPVSVPGYANPAQFATSSIGVVGGDNFQMYGVIV
                     MTTQISVETLSPITHN"
     gene            complement(323..877)
                     /locus_tag="K0W65_004391"
     CDS             complement(323..877)
                     /locus_tag="K0W65_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBI3097351.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMINGKQKTWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(874..1164)
                     /locus_tag="K0W65_004392"
     CDS             complement(874..1164)
                     /locus_tag="K0W65_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBI3097352.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQVAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(1164..1763)
                     /locus_tag="K0W65_004393"
     CDS             complement(1164..1763)
                     /locus_tag="K0W65_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBI3097353.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          446 a          496 c          421 g          471 t
ORIGIN      
        1 tggttatggg tgatcgggga gagagtttca acagagattt gagtggtcat aacgataact
       61 ccgtacattt ggaaattatc gccaccgacg acgccaatcg aactggtggc gaactgtgca
      121 gggttggcgt aaccgggtac ggaaaccggc gagcctttcg gctcccccac acagcccgcc
      181 ataaatcgcg aatgtgactg tgcaaacgat atgaaaaaag acgcgggcgc gtctcatatc
      241 gctccgtaaa catccgggac gccaatcccg acgccagatt ttgctggcgc gtgaggaata
      301 tagccccgga taacagattg agtcaacaaa cggtttttag atccccggaa gagaatgcat
      361 cacgcatcgg tagatagagc atatattcag caagatttag ccatacatct acccgactgc
      421 agcacgtccg gaaacaccac tcagggtgtg cgtcattcag caattcagcc attttacgct
      481 tagtcatccc ccgcccttca taccgttgcc ggaggacact aatcaatcca ggatgctctg
      541 ccagcacctc acttatgact ctatcaatac ataacgcctc tgcatcagta caatgcgcca
      601 gccaggtctt ttgcttgcca ttgatcatct ctcgcaaaaa cgcttccagc tcaggtttct
      661 ctattcccgc ttttttcatc ctgcgcaggg cttcattgac ggctgttttc gtcaattttt
      721 tggatgccag caactggtta aacatatttc ccgtcttacc gccgccaata tacgaccagc
      781 gcccccacat gcgcagtttt ccctgaatcc agacactttc cagcgtggtg agacgaaggt
      841 gttccccgct tttgcctgta tttgttgggt aaatcataaa taaccttcct ttctccagat
      901 ttcttgtgtg cgaaaaacac cttctgcatg catcaggcgt aattcttctt tggtgtaatc
      961 gctggttttt acccgcccgt cgattaaatc gtggcatgag ctacaggcaa tcgctacctg
     1021 catatcgtgt ggttttgtcg ctgttccgca cgtccccgcc agcctgtaat gcgccagcac
     1081 agaagtttcg ggattgtgat tgcagtagcc aggaattctg acggtgcaca tctgcccccg
     1141 cgccgcttta cgtaaatcca ccattacgca aactccagta actgcgcggc cacattttcg
     1201 acttcctccg gagaggaaaa tttacggaac aggatccagt tccacagcac attcagtaca
     1261 gatttataaa cctgctgaaa ctcggtttcg tccatgttcg caaatgcgat agattttgcc
     1321 ctgcgcccac gactaccatc aggataaata tgctcggtgt aaaatccggc ctgaatggtt
     1381 acccactcgc ggaaagcgtc aaacgacttt agcaatgccg tatcccgggt tctgcatgtc
     1441 gcaactgtat taaggtattg ctctgcggca tcactcaggg ctggagtgtg ttcccgacct
     1501 actgattcgc acaggtaatc aacgaagcct gataccagtt ttcgttcgcg aggcgtgatc
     1561 gccccaccga ccggagtcca gtaatcaaat cccagttgca ggagtttgaa aaaacgcttg
     1621 tggaatgcgt agttacgcac acgcttaaag tctgcgtgta tccactcacc tattttgatt
     1681 tgatgcaaaa aatcgcaact ctccggcgtc gccgggagaa gtaatccgga agaggtttgt
     1741 ttgaccagtt gtatatgcgc catcgtagtt ctccgctggc gcagtagaat gggtgttcag
     1801 cccgttatgt agtataccag aattaatgcc aata
//
LOCUS       DAETQL010000260         1809 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.264, whole genome shotgun sequence.
ACCESSION   DAETQL010000260 DAETQL010000000
VERSION     DAETQL010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.264"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            173..1198
                     /locus_tag="K0W65_004394"
     CDS             173..1198
                     /locus_tag="K0W65_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBI3097354.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1266..1809
                     /locus_tag="K0W65_004395"
                     /pseudo
     CDS             1266..1809
                     /locus_tag="K0W65_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc.;
                     frameshifted; incomplete; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
BASE COUNT          421 a          421 c          462 g          505 t
ORIGIN      
        1 caccattgcg gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga
       61 aatcgtgcgc aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa
      121 agacgtttcc caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa
      181 gatgatgata gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca
      241 tgctggcgcg acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga
      301 tggattacct aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgttga
      361 tgtttgtcgt ggtgttgccg ctgctgtatt tggtgacgac acgaaagtga aatatacccc
      421 actcacagca aaagaacgct tcaccgcttt acagtcaggg gaagtggatt tgctctcccg
      481 taatacgacc tggacttcat ctcgcgatgc cgggatggga atggcattta ccggcgtcac
      541 ttattacgac ggcattggct tcctgacgca cgataaagcg gggctaaaaa gcgcgaaaga
      601 actggatggc gctaccgtct gtattcaggc gggtactgat accgaactca acgtcgccga
      661 ctatttcaag gcaaacaata tgaagtacac accagtgact ttcgatcgct ctgacgaatc
      721 agcgaaggca ctggaatctg gtcgctgcga tacgctggct tcggatcagt cgcaactgta
      781 tgccttgcgc atcaaattaa gcaacccggc tgaatggatt gtcttaccgg aggttatctc
      841 taaagaaccg cttgggccgg tagttcgtcg tggcgatgat gaatggttct cgattgtacg
      901 ctggacgctt ttcgccatgc taaatgctga agagatgggc atcaattccc agaacgtcga
      961 tgaaaaagcg gctaatccag caacgcctga tatggcacat ctgttgggta aagaaggcga
     1021 ttacggcaag gatctgaagt tggataataa atgggcctat aacatcatca aacaggtgag
     1081 taactactcg gaaatttttg agcgtaacgt aggttcagaa agcccgctga aaattaaacg
     1141 tgggcaaaat aatctctgga ataacggcgg tattcagtac gcgccgcccg tgcgttaagt
     1201 cggtgtgagg taacgagcgt cgctacggcg gcgctctctt cagcgttatg ctttccgagg
     1261 tttctatgcc tcatcgccgc tcaaccgtta aaggctcact ctcctttgcc aaccctacgg
     1321 ttcgtgcctg gttattccag atccttgccg ttgttgctgt tgtcggcatt gttggttggt
     1381 tatttcaaaa cactgtaacg aatctcaata atcgtggcat tacttcaggt tttgcctttc
     1441 tggatcgcgg cgctggcttc ggtattgtcc agcatttgat cgattaccag cagggcgaca
     1501 cctacggacg cgtttttatt gtcggcttac tcaatacgct actggtttct gcattgtgta
     1561 ttgtgttcgc ttctgttttg ggcttcttta tcggtctggc gagactttcg gataactggc
     1621 tgctacgaaa gctttccaca atttatattg agatcttccg taatattgca aatcttcttc
     1681 tggtactttg ccgtgttgcg caatttgccc ggaccacgcc aagctgtgag cgcgtttgat
     1741 ctggcctttt tgagcaatcg tgggctttat attccgtcac cgcagctggg agacggattt
     1801 attgcgttt
//
LOCUS       DAETQL010000261         1760 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.265, whole genome shotgun sequence.
ACCESSION   DAETQL010000261 DAETQL010000000
VERSION     DAETQL010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.265"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..264)
                     /locus_tag="K0W65_004396"
                     /pseudo
     CDS             complement(1..264)
                     /locus_tag="K0W65_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(379..1017)
                     /gene="ospI"
                     /locus_tag="K0W65_004397"
     CDS             complement(379..1017)
                     /gene="ospI"
                     /locus_tag="K0W65_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HBI3097355.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1176..1646)
                     /locus_tag="K0W65_004398"
                     /pseudo
     CDS             complement(1176..1646)
                     /locus_tag="K0W65_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          502 a          434 c          319 g          505 t
ORIGIN      
        1 cagcaagcgc tgtaactctt ttatttgttt tatagcggca gccagttctg aggcaggaac
       61 gacatcctct ccggcagcaa cagcagtgag acttccttcc agatattatt ttttccactt
      121 aagcaacagg ctgggctgaa taccatgcaa acgcgccaca tgggacaccg tcatgccagg
      181 ctccattgtc tgctgaataa tggcaatttt ttcctgagga gttttacgtt tacgaagctc
      241 cagccccagc aaagttccag tcatctcaaa attagaacta gtgttagaca tatatccaag
      301 cctatctctt attttaagat acatccactg tctggcgttt caaggggccg gctcaaatct
      361 atgtacaggc tctcagcatc agcaaagcct cttacttttc caactactac tgttagcata
      421 gtgatcaaat tcctgatcag atacatgaat tacctgaacc gaactatttt catctacaaa
      481 tgcatctggc gatttttcta caggatcata aagattaaat ccctttcctg tttgaacatc
      541 caaaaacata attttttcac catcgttaaa tccagctatc caatgagtgg cctgttcaat
      601 agaaatcatt actacacttc cttttgaagt attttgtaaa atattttctg caatacgctc
      661 acgaaattta ctataagttt cattaggatg tgacactatg ccttcactat caatttgctc
      721 taaatgccta tgaaaaaatc cggtcatata ctctgatatt tcattaggta cgggagcctc
      781 tcttactcca taccctaaca tagccataca agcatgaagc gcacaccctg aacagttacc
      841 atccccacca gacggattta tcattttctg taaagtcgct gccaattcag aatctttatc
      901 catatcacca agacttttag agtttaaaga ttccagcaat tttttaacat ttacatttcc
      961 ctcctctgta tgtgcctcat atccagcagt tccctgtaac gacaccccat taatcatatt
     1021 gtatcctgac agtatatata ctcactatat tttcttcata acaaatatcc ctctgatatg
     1081 acaacaaaaa acggagccgg attcctgttt ttgtgaactg cagctattcg tgtctaaaaa
     1141 gtgatccact taccaccaac ggcctaatat atacagaacg gagtaaacag gactaacagt
     1201 tcccgacagg tctcatcatt ccgtggcccg aaagtgtaag ccaacacacc gccagtttta
     1261 gtgttgtacg aataccagag ccagtgttgt tgcagccttg ctgccaacga agcgccactg
     1321 ctcatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag atgttatccg
     1381 ccggggcggg agctttttaa tgtccgtatg acggtattga tgccgatttt cagtgtcctg
     1441 gcggtatcac ggccccctgc gccgttatgt gccatttcaa cgatctgctc cttaacgccg
     1501 ggttttctgg cttcataaga gtaagtgagc tggaaaacac gtttacatga gcgacaacgg
     1561 aaacgctcat gccgggaaca actacggcca tgtcgataaa cttcatcgga atgacagcgc
     1621 ggacaatgaa cagtaacggt agccatggaa aaccttaaat accagtagat taccagaaac
     1681 ttctgtcgat tgaagacatc acccgaagtt tgaattcatt cgaatacttt ttacgaggtt
     1741 cttttcgcca gtcctgtaat
//
LOCUS       DAETQL010000262         1750 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.266, whole genome shotgun sequence.
ACCESSION   DAETQL010000262 DAETQL010000000
VERSION     DAETQL010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.266"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            454..1302
                     /locus_tag="K0W65_004399"
     CDS             454..1302
                     /locus_tag="K0W65_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBI3097356.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPTMTTAHNRAQAVFLC
                     VKHSHIQIMVGRAGQPQGWPVSVVTGCSNPVRLTTHEIATSGGESFKLTIEAAIMATI
                     LTLSHPDATIENGRAVTTSVAVAEFFRKMHKNVIQKIETLECSPEFNRLNFKPVTYTD
                     AKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMI
                     PGDGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVE
                     QLLSLK"
     gene            1319..1600
                     /locus_tag="K0W65_004400"
                     /pseudo
     CDS             1319..1600
                     /locus_tag="K0W65_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          501 a          398 c          411 g          440 t
ORIGIN      
        1 agcattatat agttctactc tggcgagata cgttggcata ttcatttcct tactggttgt
       61 gtgagaactt cagtaaagat accaccaaag cccggaagtg gtgaaataaa accgggcaca
      121 acacgaaggc gcatttccgg tatccataaa gagtcggtct tgtctgtgaa atttaaatgg
      181 tgggagtgcg cttccggttg taaataacga cattgctgtg tgaagtacca gttggcggca
      241 tcggtttaat tgctggctga tgtccgccct ttttaaagtg aattttgtga tgcggtgaat
      301 gcggctatgc gcacgcggca cagttaaaag tcatgttagt ccttattggt ttgggtggga
      361 aagccaactg taattgttaa ctggttaacg tcacctggag gcaccaggca ccgcatcaac
      421 aaagttcact tcggtgatga aaggaaagag aaaatgttga atgtagctat tgaaaaccag
      481 aacgggtgga attatagtgc acctgcacct cataaaacgg gtgccgggat tgctaccccg
      541 acgatgacta ctgcgcataa ccgcgctcag gcggtttttt tatgcgtcaa gcacagccac
      601 attcagatta tggtggggcg tgcagggcag ccgcaaggct ggccggtttc ggtagtcacc
      661 ggttgtagca accctgtgcg tctcaccacc catgagattg ctacctctgg tggtgagtca
      721 tttaaactga ctatcgaggc tgccatcatg gctactatcc ttaccctttc tcaccctgac
      781 gcaaccatcg aaaatggtcg cgctgtcact acgtctgttg cggttgcgga gttcttccgg
      841 aaaatgcaca aaaacgttat tcagaagatc gaaactctcg aatgctcgcc ggagtttaac
      901 cggcttaatt ttaagcccgt cacctatacc gacgccaaag gcgaaaaacg cccaatgtac
      961 caaatcacca aaaacggctt cgttttcctg gtgatgggct tcactggcaa aaaagccgcc
     1021 gcattcaagg aagcctacat tgctgagttc gaccgcatgg aggccgaact gcgccagaat
     1081 aacaccccac ctgcagacaa gatgattccg ggtgatggtc gcactctggt tgttcacttc
     1141 gacaaattcg gcaatgtcga attcaccgaa accgttcctg atggcgcact ggtctgtacc
     1201 ctggagactt tccggtttta tctggaaaaa cagggatgga ctcttgttaa ccggggcgca
     1261 attaaaaata tgactgtgga acaattactt tctttaaaat aaaaatggag ctacggatat
     1321 gaaccctatg cttgattatt gttttacctc tgcacctcag cagaccataa cacacaccgg
     1381 aatcaaaaat ttgttatgga taatcaaaaa aaacctccag tagaaaggtg atcccgattt
     1441 aattccctgt gcttatggcc tgtgtgacag acaagtaagc cctgataagg cttacactgc
     1501 tgaacttatt tacatgcacg gaaccatgat gaggaaaaag aaacgcaaat attgtagtga
     1561 aatctgcgcc gaaaaagacc agatggcaca cgaactttaa ttaactgact attcgaaact
     1621 gaatttatgc cagcaatggc agggattcgc tcaaccttaa ttaaggagaa aaacatgaac
     1681 accaattatg aagccactgt tgcaactact gataacattg ttcacgaggt ttacctggaa
     1741 ggaaagcata
//
LOCUS       DAETQL010000263         1718 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.267, whole genome shotgun sequence.
ACCESSION   DAETQL010000263 DAETQL010000000
VERSION     DAETQL010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.267"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(110..577)
                     /locus_tag="K0W65_004401"
     CDS             complement(110..577)
                     /locus_tag="K0W65_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBI3097357.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="K0W65_004402"
     CDS             complement(643..882)
                     /locus_tag="K0W65_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HBI3097358.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="K0W65_004403"
     CDS             complement(1017..1622)
                     /locus_tag="K0W65_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3097359.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          550 a          378 c          305 g          485 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagcaa
//
LOCUS       DAETQL010000264         1706 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.268, whole genome shotgun sequence.
ACCESSION   DAETQL010000264 DAETQL010000000
VERSION     DAETQL010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.268"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..87)
                     /locus_tag="K0W65_004404"
     CDS             complement(<1..87)
                     /locus_tag="K0W65_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HBI3097360.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLD"
     gene            complement(767..841)
                     /locus_tag="K0W65_004405"
     tRNA            complement(767..841)
                     /locus_tag="K0W65_004405"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Gly,seq:tcc)
     gene            complement(845..919)
                     /locus_tag="K0W65_004406"
     tRNA            complement(845..919)
                     /locus_tag="K0W65_004406"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(884..886),aa:Thr,seq:tgt)
     gene            complement(922..998)
                     /locus_tag="K0W65_004407"
     tRNA            complement(922..998)
                     /locus_tag="K0W65_004407"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(962..964),aa:Arg,seq:tct)
     gene            complement(1000..1076)
                     /locus_tag="K0W65_004408"
     tRNA            complement(1000..1076)
                     /locus_tag="K0W65_004408"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1040..1042),aa:Ile,seq:cat)
     gene            complement(1266..>1706)
                     /locus_tag="K0W65_004409"
     CDS             complement(1266..>1706)
                     /locus_tag="K0W65_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBI3097361.1"
                     /translation="GGKTGNMFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLR
                     DMINGKQKSWLAHCTDAEALCIDRVISEVLAEHPGLISVLRQRYEGRGMTKCKMAELL
                     NDAHPKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          467 a          452 c          347 g          440 t
ORIGIN      
        1 atccaaccac tgcaaaaacc agtagccggc actgccggcg gaggtgccgt aggcaatgcc
       61 tgttgatatt ttttccatct gatacatatc ccgccccccc gacagacctg tgctatcgga
      121 aagaaaaaag gccatcagca aaactctgat ggcctgaatc acctttacca atattgtatg
      181 aaaaaacacg cacgactaat tgacaataat tttcatttcc attaaaaaag atcgcgtagc
      241 attcttaatt catgaggaac tttacccgcc agcaatctga gtagcgaaag ctgttcatcc
      301 ccaatgtttt tgctggcggg tccttttttc tacggtcctc ctccccggag gggcatttca
      361 ttatttttct aatcgttact ggtgagaagg cggaagaaaa agccagttct tcgcccctaa
      421 ataacagcat gattccagtc catgcaaaat ctgatttcca gctcacacat accgagcact
      481 gtacaaaaaa ccgccagcca aggcagttaa gagtgtgttc ccggggtttg cttagaatat
      541 tttgtaagtt gtccgaagag atatttacaa caccagaatg atgcattatc agtcccctgc
      601 cagaggttca ctacgtaacc tgaccgacaa tgcaccattc tggtgctgaa aaaagagcac
      661 tggaactgca acaaaacaac atcacagaac aggagatggt aaggagtcag acattcccac
      721 acaatattgt gtcagtgcaa ataacaacct tcgtctcaaa tctcgctgga gcgggcagcg
      781 ggaatcgaac ccgcatcatc agcttggaag gctgaggtaa tagccattat acgatgcccg
      841 catatggtgc gactaccgga atcgaactgg tgacctactg attacaagtc agttgctctg
      901 cctgctgagc taagtcggcg ctggcccacc accgaggact cgaacctcgc accgtcaact
      961 tagaaggttg atgctctatc cggatgagct agtggtggtt ggtggccctt gctggacttg
     1021 aaccagcgac ctggcgatta tgagtcgctc gctctgacca actgagctaa agggccggag
     1081 gcagaataat aaccatatgt catcacatct gcaaactcat ctgatcacca gcacgtttaa
     1141 cgtcctgtgc cgtttttcag gtataaaaaa aaccgcataa agcgggctct ttcaaatgtc
     1201 catctctgct attcgcctcg cggtacagct ttgcgaagcg tacaagaatt gaagcagttt
     1261 ttacgtcaaa aagcaataac ttttttctct ataccaaaag ccataaccat tggtttgtac
     1321 aaaataaatt ctgccacctt tagccaatgc tcaatgcgtc tttcacaggt tcttaaactc
     1381 catttcgggt gtgcatcatt cagcagttca gccattttgc atttggtcat cccccgcccc
     1441 tcataccgtt gccgaaggac gctaatcaat cctggatgct ctgccagcac ctcacttatg
     1501 acccgatcaa tacataacgc ctctgcatca gtacaatgcg ccagccagct cttttgctta
     1561 ccgttaatca tatcccgcaa aaaagcctca agttcagact tgttcagacc tgcttttttc
     1621 atcctccgga gcgcctcgtt aattgccgtt tttgtcagct ttttagaggt caacatcagg
     1681 ttaaacatat tccccgtctt accgcc
//
LOCUS       DAETQL010000265         1656 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.269, whole genome shotgun sequence.
ACCESSION   DAETQL010000265 DAETQL010000000
VERSION     DAETQL010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.269"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(570..749)
                     /locus_tag="K0W65_004410"
                     /pseudo
     CDS             complement(570..749)
                     /locus_tag="K0W65_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            798..1656
                     /locus_tag="K0W65_004411"
                     /pseudo
     CDS             798..1656
                     /locus_tag="K0W65_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          431 a          340 c          450 g          435 t
ORIGIN      
        1 ttagggtagt cactatattt gcgactgagc tggtcgctat ttttaaccag gctgaaaaat
       61 tgagtcaata tatcgaaagc ggtagttggc gggcataagt atgtaatgat gatgctgcac
      121 tggtgttgaa tattattcac gccaaacttt cctcattgag caatgtcgtc gcaagtggcc
      181 tcaatgattt tagaccatta tcagaggtcc ggagcggcgg gaagagattt attgctgtgg
      241 aataattgtg gtactgatga ttatttatat cccggattgg tagggaaaag cttttttcat
      301 gcactatttt tgcaaatatt tctatatggg aagtggtttt gttacagatt aaatttataa
      361 agaaatgcat ttggggtgtt gggttgaaga gtagctggta gatgttattg tgccagcagg
      421 ggcaggggaa tgtgccgtat cgatatgctt tggcaattgc gtgtgccgca gatggagatg
      481 aaatgccaat aaaaaagcct gagttcgatg agggacgaac tcaggctggt gttatgtttt
      541 acagtaacac gcctcggcgc gttggctgat tagaactggt agacgatacc aactgccagt
      601 tggtcgtcgg tgttcagtcc atgagcgaga acataatcac tcttatcaag caggttgatc
      661 tgataagcgg tatatatgtt catattctta ttgaagtaat accaggtacc gatttcaata
      721 tagttcagac gatcagaatc tttgtaacct gtagactggc cccctgaatc tccagacaac
      781 caatatcact tatttaagtg atagtcttaa tactagtttt tagactagtc attggagagc
      841 agatgattga tgtcttagga ccggagaaac gcagacggcg tactacacag gaaaagatcg
      901 ctattgttca gcagagtttt gaaccgggaa tgacggtctc ccttgttgcc cggcaacacg
      961 gtgtggcagc cagccagtta tttctctggc gcaagcaata ccaggaggga agtcttactg
     1021 ctgtggccgc aggagaacag gtcgttcctg cctctgaact tgctgctgcc atgaagcaga
     1081 ttaaagaact ccagcgcctg ctcggcaaga aaacgatgga aaatgaactc ctcaaagaag
     1141 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
     1201 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
     1261 cagacgaacc gatgactgga aagatggtcg ccgcagccgt cactcagatg atacggatgt
     1321 gcttctccgt atacaccatg ttatcggaga gctgcccaca tatggttatc gtcgggtatg
     1381 ggcgctgctt cgcagacagg ccgaacttga tggtatgcct gcgatcaatg ccaaacgtat
     1441 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaactcgctg taccgccatc
     1501 gaaacgggca cataccggca gagtggctgt gaaagaaagt aatcagcgat ggtgctctga
     1561 cgggttcgag ttccgctgtg ataacggaga aaaactgcga gtcacgttcg cgctggactg
     1621 ctgtgaccgt aaggcactgc actgggcggt cactac
//
LOCUS       DAETQL010000266         1602 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.270, whole genome shotgun sequence.
ACCESSION   DAETQL010000266 DAETQL010000000
VERSION     DAETQL010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.270"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(334..480)
                     /locus_tag="K0W65_004412"
                     /pseudo
     CDS             complement(334..480)
                     /locus_tag="K0W65_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            534..716
                     /locus_tag="K0W65_004413"
     CDS             534..716
                     /locus_tag="K0W65_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HBI3097362.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(817..1146)
                     /locus_tag="K0W65_004414"
     CDS             complement(817..1146)
                     /locus_tag="K0W65_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBI3097363.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1318..>1602)
                     /locus_tag="K0W65_004415"
     CDS             complement(1318..>1602)
                     /locus_tag="K0W65_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097364.1"
                     /translation="HFVLLNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLN
                     DAVEELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          435 a          368 c          377 g          422 t
ORIGIN      
        1 tagcaatgtc tgtcgcacaa caaccacaat gtcacaaata aaaaatgaga ataaccagtt
       61 ccctgaaggc gacgtagccc ggtacaagag taacccacgt tagcgcttgg cgacggcgga
      121 acaaagctga ttcgcgacgt aagaaagtgg caggctcata tgacggacct ctgtaatgcg
      181 gtatccaacc cgcgaatatc agcatgattc accgtcggat ttactgctac gtcgccctca
      241 gggaattaaa cctaaaaaga aaatcgtcaa aattagagat tccggtttga cacggaaagt
      301 catatcagcg tcaacggagc accgtattga cgcttattta ttggtgagaa ctacgttcca
      361 tggcaggagt tcgtcaacac ggttggaggg ccattcaggc agtatgctca gaatatggcg
      421 cagatacgct tccggatcga taccgttcag acggcaggtg cctgcttttc cggcaacaag
      481 caggaatctc agcgctgaag caggaattag aggtaaaaac gccctaccgg gccatgaatc
      541 acccggtcat tggagtagta accaaagcag atttagccag catggaacaa atatctttgg
      601 tgaaaagttg gttacgggaa gctggagcgc acaacgtgtt agtaaccagt gcagttaata
      661 acaatggagt tacagaactt tttgccttgc tgcatacaga agaaggctgt tgttaatttc
      721 gattggttct cagatgagaa tgatgacggt gatacacaga taagaatcaa tgagcaatga
      781 gggttgccgg gcaaccctca ttgaataaaa cgggaattac ttcgcttcgc cgtttttcat
      841 ttcgcccata gctttcagct ttttggagat atcgcggcgt tctttggaga gctcggcatt
      901 tttgatgatg taatcgtcaa cgcgatcttc atagtcacct ttcatgctgg cgatgatgcc
      961 ctggattgct tcaacgctca tccctggctt gatgtaatcg ctcaggttgt ccagcaacag
     1021 gacgcgcttc tggttgtcac ggatcttttt ctcaacgtcc tgaatttcac gttgcagttt
     1081 gttcttacga cggaacagac gaacaaattc cagtacgtcc tggaatgaag gcttggtagt
     1141 ttccattttt atacccctga taatgtgaga gtcggattcg tttaagcaac cgctattcgt
     1201 tagggccaac attactcata gctgccgcag atgacaatgc ttttatccct tttcactatc
     1261 atacccttta tccttgctga atcgaagcag cagcaagatg attctgaagt tcaggaatta
     1321 tttgcgcaag gcccggcaaa tcagattcga tagcaactca agctgatgtg ccgtttccat
     1381 gttcagccac acataaccat agattggtgt ttccacgact ttcaggtcat ggtggttatc
     1441 gagcaactga cgcagctctt ccacagcatc gttcaatttt tccgtattgg caaaaaccgg
     1501 ctgcggatta ccttcgtaca gcgcatgaac aaggctcagc agtatttgct gcatcatgtg
     1561 ctgggtatca caaagttttt gcgcgttcaa taacacgaaa tg
//
LOCUS       DAETQL010000267         1597 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.271, whole genome shotgun sequence.
ACCESSION   DAETQL010000267 DAETQL010000000
VERSION     DAETQL010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.271"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..636)
                     /locus_tag="K0W65_004416"
     CDS             complement(<1..636)
                     /locus_tag="K0W65_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBI3097365.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGA"
     gene            complement(750..1322)
                     /locus_tag="K0W65_004417"
     CDS             complement(750..1322)
                     /locus_tag="K0W65_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HBI3097366.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          474 a          337 c          319 g          467 t
ORIGIN      
        1 tgcgccgtaa tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa
       61 aggtaacgtg agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac
      121 gatgacatcc atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc
      181 ttcccggcgc catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg
      241 tcgccagaac agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt
      301 gctcgctgat ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg
      361 caatgacaca cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg
      421 tgatactggc ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc
      481 tacccaagcc tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt
      541 atcagcatta ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt
      601 taatgctggt tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac
      661 tgttgtttta aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa
      721 gccaactgaa taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa
      781 tcagcgttcg ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac
      841 ttcatttcag ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta
      901 acctgcgaat tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct
      961 tgtcctgtac cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg
     1021 tcgccgtcgc cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt
     1081 gccgggcaat tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct
     1141 gaaaatgcat ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta
     1201 acttcacaag tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt
     1261 gcatgcccgg ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc
     1321 ataaaaactc cattgatata atatatcctt taccatgaat catcacttat attaataagt
     1381 gtgtttttga ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta
     1441 attacgcaaa aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca
     1501 agtttgttat cagacattaa tgataattca gaatatattt atatagaaag atagatgaat
     1561 ttattgatgt cagttataaa aaagggccac cggtcgg
//
LOCUS       DAETQL010000268         1581 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.272, whole genome shotgun sequence.
ACCESSION   DAETQL010000268 DAETQL010000000
VERSION     DAETQL010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.272"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="K0W65_004418"
     CDS             complement(<1..1266)
                     /gene="yiaN"
                     /locus_tag="K0W65_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HBI3097367.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLK"
     gene            complement(1269..>1581)
                     /locus_tag="K0W65_004419"
     CDS             complement(1269..>1581)
                     /locus_tag="K0W65_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HBI3097368.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          482 a          400 c          406 g          293 t
ORIGIN      
        1 tttcaaaggg aggatgatga gatcgggaat aaaaacaaat acgactaata atgaatataa
       61 aaccaggacg taagggaaaa cgcctctgac tgcatcatcg aatttgagtt ttgccacccc
      121 tgaaataacg ttaagtacat taccgatagg cggtgtaatt aagccgattg agcagttaat
      181 gataaacatg acaccgaaat aaatcggatc gattcctgcc tctttaacta aaggcattaa
      241 taccggggta agaattaata ccgtcggcgt taaatccatg accatgccga caatcaggat
      301 agccaccata atgacgataa acagcagacg cggtgaatcg accaacggtt gcagtaaatc
      361 agaaaccatc atcggcagtt cagcaatggt aatcagccag gcggaaactt gtgccgaggc
      421 caccagaaac ataaccactg aggtggtttt cgccgcgcca atgagtacat gccaaagtgt
      481 ggcgaaggtc atttcacggt aaataactgt ggcgacaaac agcgcataga aagcagcaac
      541 ggccccggct tcggtagggg taaacagacc ggagcgaaac ccgccaataa tgatgactgg
      601 aagaaacagc gcccagatac cggagacaaa agagtgccag atttcctgca tcgttgcttt
      661 ttgctggcgg ggtaaattaa ggcgactggc ctgccgccac caggtaagca tcagcgttgc
      721 gcccatcatt atgccagggg caatgcccgc cataaacagc ttactgatgg ataatccgct
      781 ggaaacaccg aagataataa acggaatgga agggggaata attggcgcga taatgccgcc
      841 agaggcaatc agccccgccg cccggttgac cgggtagttg gcgctgcgca tcatcggcac
      901 cagtagagcg gcaacggcgg cggtatccgc aacggcagaa ccagaaaggc tcgccataat
      961 catagctgcc agcacgccga cgtagcccag cccgcctggt ttatgcccca ccagtttcat
     1021 cggcaggtca acaatgcgtt ttaacaagcc gcccgcattc atgatttcac ccgccagcac
     1081 aaagaacgga atcgccagga gggagaagct atcggctccg ttcaccagcg tttgtgccat
     1141 gatctggaca tcaaacatgt ccagccagaa cattaacgcc gccccgcaca acaacagtgc
     1201 ccaggcaata ggcaaaccga tagcaatacc acccaacaga cagcccagaa aaatcagcac
     1261 agccatgatt aagctccttg cggtggcgac gttagagaat tgctacgcgt gatgagttga
     1321 tataaatgac gcagttcaaa gaatgcgata acgaggctgg tgggcagaca agcggcatac
     1381 atcaggccga tgggcaaacc gaggatcggt gaataatcgc tccagtcctg aattgttttt
     1441 agcgttgcgc cccaggccag tgcgccacaa ataaataaga ttaaggaatg ggtaaccaga
     1501 gcgactcgtc gttgccatgc gggggagagt ttctccacca gaaaggtgac ctgaacgtgg
     1561 gcgttatcca taaaagccac a
//
LOCUS       DAETQL010000269         1559 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.273, whole genome shotgun sequence.
ACCESSION   DAETQL010000269 DAETQL010000000
VERSION     DAETQL010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.273"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            39..>1559
                     /locus_tag="K0W65_004420"
     CDS             39..>1559
                     /locus_tag="K0W65_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBI3097369.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSLRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKLLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPAAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTDITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWEALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWLFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPA"
BASE COUNT          378 a          429 c          456 g          296 t
ORIGIN      
        1 cgctggaatc cgcatataac ccgttgtaaa gtgaggatat ggacacctca cttgctcatg
       61 agaatgcccg cctgcgggca ctgttgcaga cgcaacagga caccatccgc cagatggccg
      121 aatataaccg cctgctctca ctgcgggtgg cggcttatgc ttccgaaatc aaccggctga
      181 aggcgctggt tgcaaaactg cagcgcatgc agttcggtaa aagctcagaa aaacttcgcg
      241 caaaaaccga acgacagata caggaagcac aggagcgaat cagcgcactt caggaagaaa
      301 tggcggaaac gctgggtgag caatatgacc cggtactgcc atccgccctg cgccagtctt
      361 cagcccgtaa actgttaccg gcctcacttc cccgtgaaac ccgggtcatc cggccggaag
      421 aggaatgctg tcctgcctgt ggtggtgaac tcagttctct gggatgtgat gtgtcagagc
      481 aactggagct tatcagcagc gcctttaagg ttatcgaaac acaacgtccg aaactggcct
      541 gttgccggtg cgaccatatc gtgcaggcac cagtaccttc aaaacccatt gcacgcagtt
      601 atgccggagc ggggcttctt gcccatgttg tcaccgggaa atatgcagac catctgccgt
      661 tataccgcca gtcagaaata taccgtcgtc agggcgtgga gctgagccgt gccacactgg
      721 ggcgctggac aggtgctgtt gctgaactgc tggagccact gtatgacgtc ctgcgccagt
      781 atgtgctgat gcccggtaaa gtccatgccg atgatatccc cgtcccggtc caggagccgg
      841 gcagcggtaa aacccggaca gcccggctgt gggtctacgt ccgtgatgac cgtaacgccg
      901 gttcacagat gcctgcggcg gtctggttcg cgtacagccc ggaccggaaa ggcatccatc
      961 cacaaaatca cctggccggt tacagcggag tgcttcaggc cgatgcttac ggtggctacc
     1021 gggcgttata cgaatccggc agaataacgg aagccgcgtg tatggcccat gcccggagaa
     1081 aaatccacga tgtgcatgca agagcgccaa ccgatatcac cacggaagcc ctgcagcgta
     1141 tcggtgaact gtatgccatc gaggcagaag tccggggatg ttcagcagaa cagcgtctgg
     1201 cagcaagaaa agccagagct gcgccactga tgcagtcact gtatgactgg atacagactc
     1261 agatgaaaac actgtcgcgt cactcggata cggcaaaagc gttcgcatac ctgctgaaac
     1321 agtgggaggc cctgaacgtg tactgcagta atggctgggt ggaaatcgac aacaacatcg
     1381 cagagaacgc cttacgggga gtggccgtag gccggaaaaa ctggctgttc gcgggttccg
     1441 acagcggtgg tgaacatgcg gcggtgttgt actcgctgat cggcacatgc cgtctgaaca
     1501 atgtggagcc agaaaagtgg ctgcgttacg tcattgaaca tatccaggac tggccggca
//
LOCUS       DAETQL010000270         1518 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.274, whole genome shotgun sequence.
ACCESSION   DAETQL010000270 DAETQL010000000
VERSION     DAETQL010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.274"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..528)
                     /locus_tag="K0W65_004421"
                     /pseudo
     CDS             complement(1..528)
                     /locus_tag="K0W65_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(579..1154)
                     /locus_tag="K0W65_004422"
                     /pseudo
     CDS             complement(579..1154)
                     /locus_tag="K0W65_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1158..1518
                     /locus_tag="K0W65_004423"
                     /pseudo
     CDS             1158..1518
                     /locus_tag="K0W65_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          332 a          432 c          388 g          366 t
ORIGIN      
        1 tgctgaacat ccccggactt ctgcctcgat ggtatacagt tcaccgatac gctgcagggc
       61 ttccgtggtg atgtcggttg gcactcttgc atgcacatcg tggatttttc tccgggcatg
      121 ggccatacac gcagcttccg ttattctgcc ggattcgtat aacgcccggt aaccaccgta
      181 agcatctgcc tgaagcacac cgctgtaacc ggctaggtga ttctgtggat ggataccttt
      241 ccggtccggg ctgtacgcga accagaccgc cgggggcatc tgtgaaccgg cgttacggtc
      301 atcacggaca tagacccaca gccgggcagt tcttgtttta ccactgcccg gctcctggac
      361 cggaacgggg atatcatccg catgaacttt accgggcatc agtacatact ggcgcaggac
      421 gtcatacagc ggctccagca gttcagcaac ggcaccggtc cagcgaccca gtgtggcacg
      481 gctcagctcc acgccctgac gacggtatat ttctgactgg cggtataact gtcaacgacg
      541 gatgaaaagt ggctcttccg caacctgggt ggagattttc atgccagtgg actcattacc
      601 ctctgcctga gtagttcaaa tcctgctcga ccatacatct ggcgtttcag catcttcttc
      661 aggcgattta catgaccttc gacaacacca ttactccagc gactactgat tgcctcacat
      721 atagctgcag catcagcttc catccccgcc gccacgcgcc gaaattctgc tgaaccgctt
      781 tcgtggactc gagtgaacca actgcttagc tgtgatttat tttgggtttt cagaatacgg
      841 tagaactcga gtgccagttg ctgcgctatt ttcagctcag gttctttttc acacatcaga
      901 ctaataaatc gggaagcata attttcttca tccctggtta ttctccaggg catcaaccag
      961 cggcttaccc gggagactga aggaagtctg acggcagtag taaccggtgg gttccagcct
     1021 ttacgccatt tggcaaccgc atccctgacg atggtttcac tgcctgtaaa tccccgggcc
     1081 accatttccc gccatatccg gctggcgtta taattaccac tttctcgttg ctcttttaac
     1141 cactccctcc atggtgtcag cgaaaaacga aaccgctgtt gaaatccctg gaaagctggt
     1201 tgcggaaaag atgaagaccc agtcgcgaca ctcagagttg gcgaaggcgt tcgcgtacgc
     1261 acttaaccag tggccggcac tgacgtacta tgcgaacgat ggctgggtgg aaatcgacaa
     1321 caacatcgct gaaaatgcct tgcgggcggt cagtctgggt cgtaaaaact tcctgttctt
     1381 cggctctgac catggtggtg agcggggagc gctactgtac agcctgatcg ggacgtgcaa
     1441 actgaatgac gtggatccag aaagctacct tcgccatgtg cttggcgtca tagcagactg
     1501 gccggtcaac cgggtcag
//
LOCUS       DAETQL010000271         1510 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.275, whole genome shotgun sequence.
ACCESSION   DAETQL010000271 DAETQL010000000
VERSION     DAETQL010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.275"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(157..639)
                     /locus_tag="K0W65_004424"
     CDS             complement(157..639)
                     /locus_tag="K0W65_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI3097370.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(630..932)
                     /locus_tag="K0W65_004425"
     CDS             complement(630..932)
                     /locus_tag="K0W65_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBI3097371.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1204..1476
                     /locus_tag="K0W65_004426"
     CDS             1204..1476
                     /locus_tag="K0W65_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097372.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          429 a          378 c          322 g          381 t
ORIGIN      
        1 ccttcactga tcgaaagctg attttcagga accgttttga cggcttcggc tttaaactcc
       61 gtagagtaac gttgggtttt tctgctcatt attggctcct tctgatgcca ttctatttca
      121 ggaaggagtg tccgttaaac tcaggctacc tcagactcag actttataaa acaaggtatt
      181 aggtgccaca actgactgaa caaaaccaaa acgctcataa aatgctctgg cgtcatcatc
      241 aattgcatgt accataacag ccttaatgcc aacctgatca gccagattga caacccggtg
      301 aatggcatca cttagtagcc atttaccgaa accatgcccc tgggtacaaa catcaacagc
      361 cagacgccct aacagaacaa cagggacagg attgggcatg ttatgccgca aactgcgacc
      421 aagctcggca tgagtgacgg aaccagtagc taatgagtag tatcccacaa tacgtaaagt
      481 atcctccaag caaataacga acgtgcgaga agcattgagc atctggtttt tcatagcccg
      541 accacgtaac cagttactta gcacctcatt tccacagtca aatggctgaa gtatatgctc
      601 atccgtcaac aatgctggcg cggtgacatt tatttccatt caggctttac cgccatcaga
      661 cgttcacgcc cttctgcatt ctgcacagga gcttccagtt gcgtaataaa cgaatcataa
      721 cgttcgttat ccagcacaaa taaacgttga tccaggataa tgtttttagc ttcctggtac
      781 gccagttcca tgatgaaatc ggttcggttc ttattgacca agctcgcagc ataatcaata
      841 acggctcgtt cctcatcagt tgcccggatg ttaatctgat gttctcgcgg tgttttacgg
      901 acgcttgcag ctgtagacat aaaaacctcc attggtaaca atttgctatg caattaatgt
      961 tatcgcaaat ggtaacagaa cgctatacag aatcacttac gggaaaagcg atcccctgat
     1021 aaaaacaatg gcagagacct gaacattgtg acgatgatct gtgtcggcgc acaaatcatg
     1081 tctgattacg gccgtttaat gtgattttga taatgaaatg cctgaaccac ggcagcctgg
     1141 cggctgcaaa tcggggtgtg gcagaatatg agctttcaga gtaaccatcc agaatgagtt
     1201 cccatgtcac acaacctgga gcaccagaaa gtccacacca gaatggtgaa agaggttctg
     1261 aaagccgtcg ccagagccaa taatcaccct tataagtccg tatttgcaga ctttatcacc
     1321 ggtcatccct cctgcacggt ctgtttctgg gaaaccttcc acaaaatgta cccggattca
     1381 ccgtacgaat acgtcacgtt ctgccatacc tgccgtcgtt ttgatttata cgaaacagaa
     1441 gcggagatga aggcggatga cccgaagtgg tggtgatttt cagccccgac cgctgaaacg
     1501 cctgttcaca
//
LOCUS       DAETQL010000272         1405 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.276, whole genome shotgun sequence.
ACCESSION   DAETQL010000272 DAETQL010000000
VERSION     DAETQL010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.276"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(427..>1405)
                     /gene="ospC4"
                     /locus_tag="K0W65_004427"
     CDS             complement(427..>1405)
                     /gene="ospC4"
                     /locus_tag="K0W65_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HBI3097373.1"
                     /translation="DKIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLN
                     KTHHTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVS
                     RYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRII
                     NFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYS
                     LGNKKADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEYFI
                     NNGLVDVNKKFQKVNSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          413 a          271 c          230 g          491 t
ORIGIN      
        1 ccttcactga tcgaaagctg attttcagga accgttttga cggcttcggc tttaaactcc
       61 gtagagtaac gttgggtttt tctgctcatt attggctcct tctgatgcca ttctatttca
      121 ggaaggagtg tccgttaaac tcaggctacc tcactcccgt aaagacagga gtcaagttcc
      181 cgctccgaca taactgcgtg caatggattt tgaaggaacc gtcagcccat tccccggagg
      241 caaacatggg ctggcaaaag tgtagcggta ttaggcacag ctatttagga aagagggacc
      301 atctccatct cattaaatgg accacgccag acaattcact gtcatgctga tgctgcactg
      361 tggggacaga atcactcatg atgacttcga taatcgacga cattattatt tggccgagct
      421 tttaggtcat atctcaaatc gtttgcctaa tattgctcca ttttttaata aaaaatcaat
      481 catttttgaa tctttgcttt tcattgcatt gtcaagcata gtgtccccac tatttacttt
      541 ttgaaatttt ttgtttacat caaccaatcc attattgata aaatattcta gcactttata
      601 gttggcacca tctttactaa gtaagtattc aacatcatac agattacaat atctattgtt
      661 tttcattttc tccatctctg cgacatcttg ttttgtaaaa ttaaatttag atagtaaata
      721 aagtgctata tcagcttttt tattgcctaa agaataagca agggcctgat gagccatttt
      781 tttatcagtc actttattgt taatctcttc ataattagat gctttaatcg catcctccaa
      841 agatatgtct ctgagtttta tttttttgaa gttatctgta cttagcattc ttggtatatg
      901 atactcaagc tgaaacacaa agtttataat tctatccaga ctaacaggat caatattttt
      961 gttataacaa aactctctga atccttgatc tttactcttt cttataaaat caataagatg
     1021 caacccaatt ccaagcctca tatctttagt gttaaatatt ggaacatcag ttttcccctg
     1081 attaccatgc acatacctgc taacttcatc cacaacctct ttaaagttat ctaagaaaaa
     1141 tgattggtgc tcatggtaaa aaacaggtgg aatagcatta tcaaagtgat cggttaatgt
     1201 catatatcca tatggagagt catgatcaat gatatacgca ttggcgccaa aatctacagt
     1261 atgatgtgtt ttatttagtg ggagtttttc ttgatgattg gaaatttcga ccccaaaaaa
     1321 aacaaaatcg tggttagaga tttctttaat atcctcagag tatgtattct tatgtgtttg
     1381 tatattttca gactgaattt tatca
//
LOCUS       DAETQL010000273         1404 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.277, whole genome shotgun sequence.
ACCESSION   DAETQL010000273 DAETQL010000000
VERSION     DAETQL010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.277"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..167
                     /locus_tag="K0W65_004428"
     CDS             <1..167
                     /locus_tag="K0W65_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBI3097374.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="K0W65_004429"
     CDS             152..790
                     /locus_tag="K0W65_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HBI3097375.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1018..>1404
                     /locus_tag="K0W65_004430"
     CDS             1018..>1404
                     /locus_tag="K0W65_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097376.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          347 g          393 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata
     1201 tttattaata aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa
     1261 agaagagata tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta
     1321 tttgatcatg acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagt
//
LOCUS       DAETQL010000274         1387 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.278, whole genome shotgun sequence.
ACCESSION   DAETQL010000274 DAETQL010000000
VERSION     DAETQL010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1387
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.278"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..785)
                     /locus_tag="K0W65_004431"
     CDS             complement(<1..785)
                     /locus_tag="K0W65_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBI3097377.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWV"
     gene            complement(940..1098)
                     /locus_tag="K0W65_004432"
     CDS             complement(940..1098)
                     /locus_tag="K0W65_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HBI3097378.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            complement(1169..>1387)
                     /locus_tag="K0W65_004433"
     CDS             complement(1169..>1387)
                     /locus_tag="K0W65_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HBI3097379.1"
                     /translation="SSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARVRENITL
                     GVQAGYAHSVSGSSAEGYNGQATLNVTF"
BASE COUNT          300 a          377 c          380 g          330 t
ORIGIN      
        1 acccacaatg cgcggttgag cttaatatcg gtgtcgatgt tgtgaatggc acgggtgtgg
       61 atacgttttc cttttgcact gcggccggaa atcccgcctt tcagcatatt ctcctggatg
      121 gtctgatatg cgctccacag gtctttaccg taatcctccc tgcgtcgtgg tgtcagaatg
      181 tcagcggtgg tgactggctg atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc
      241 agcgcctggc gtgccggtgg cggcaggacc agcgactgca tggcatcacg cttttcctca
      301 atccggtcaa aaacccccac cacttcgtaa gccccttcga taactttctc caccacattt
      361 ccccggtgtg gaacacgcac ttcccccaga gactgaccgc agacgcagcc attctggcag
      421 acgaatctga agtaacccgg cagcatctgg tagctggagg taccgtcatg agagttgagc
      481 agaataattt cagggacatg ttctccgttt atctctccgg cccgccgcag acgcagcatg
      541 tgttttgtgt atccccggcg gcccgggtcg cgcacgcggg tctggcaggc gaagaatggc
      601 tgaaaacctt cctgctgcag gctttccagt acggtgatgg tgggaatgta cgcatatcgt
      661 ttactgcggg aagtatgccg gtcttcaccg aaaatactgg gaacatagtg catcagttct
      721 tcgtgtgtca gcggacggtc acggcgtatc tggttcgcat aaccaaaacg actggctagt
      781 cgcataattt gctccttatc ggtggttacg atttactggt gtaataaatg aaaaagctgc
      841 gtctcccgga gaagacgcgg cctgacagat gaaatgaatg acgtttattg tctgagaagc
      901 ccttaactgg cgagctgagt attaagctgt gttccggcat caccagcgca actgaccttc
      961 agcattacgg ataaccagcc gggaatatgt tccctggtca tcttcagtaa acacattgcg
     1021 gtaagctgtt ttgacggcaa cagcctgttc gcgtgagaaa gggccttcgg gcaataaacg
     1081 tgatgtacaa cccggcatat ctgttgctcc ctgaaagtaa aaagccccgg tcatgatgac
     1141 cggggcctga aggagagtga cctgattatc aaaaagtcac attcagcgtg gcctgaccgt
     1201 tataaccttc agcgctgctg ccgctgacgc tgtgggcata accggcctga acgcccaggg
     1261 tgatattttc ccggacacgg gcttccagtc cggcctgcag gtccagtgac gtgccattcc
     1321 gggacggtga gaacgtcatg ttactgccgg cggcggctgt ccccatgctc atgtcacccc
     1381 gggagct
//
LOCUS       DAETQL010000275         1338 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.279, whole genome shotgun sequence.
ACCESSION   DAETQL010000275 DAETQL010000000
VERSION     DAETQL010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.279"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..253
                     /locus_tag="K0W65_004434"
                     /pseudo
     CDS             1..253
                     /locus_tag="K0W65_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            746..>1338
                     /locus_tag="K0W65_004435"
     CDS             746..>1338
                     /locus_tag="K0W65_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBI3097380.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRG"
BASE COUNT          325 a          312 c          411 g          290 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgtcagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccagggt ctgttcgcgc cgaaaggtgt
      661 tcggcctgag acgttgctcc gacaggttaa caccgtctcg ttgccggaca gtgcgatccc
      721 gccaggcttc agggggtaac agaccatgaa aaatattgcg acaggaggcg ttctggagcg
      781 tatccgcaga ctgaccccac cacatgtaac cgccccattc agaacggttg cggagtggcg
      841 cgagtggcaa cttgctgaag gccagaaacg ttgcgaggag atcaaccgcc tgaatcgtca
      901 gttgcgggtg gaaaaaatcc tgaaccgctc cggcatccag ccgttgcacc ggaagtgttc
      961 gtttgcgaat taccgggcgc agaacgacgg tcagcgacat gcactgagtc aggcgaaatc
     1021 catagctgac gaactgatga caggctgtac gaattttgtg ttcagcggta agcccggcac
     1081 cggaaaaaat catcttgcag ccgcgattgg caaccggctg atggcgaagg ggcgtagcgt
     1141 gattatcgtc accgtgtccg atgtcatgag cgtgttgcat gagagctacg acaacggcaa
     1201 atccggtgaa aaatttttac aggagctttg tggtgttgac ctgctggtcc tggatgaaat
     1261 tggcatgcag cgggatacga aaaacgagca ggtggtactg aaccagattg ttgatcgccg
     1321 gacggcatcg ttacgcgg
//
LOCUS       DAETQL010000276         1336 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.280, whole genome shotgun sequence.
ACCESSION   DAETQL010000276 DAETQL010000000
VERSION     DAETQL010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.280"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(207..362)
                     /gene="hokD"
                     /locus_tag="K0W65_004436"
     CDS             complement(207..362)
                     /gene="hokD"
                     /locus_tag="K0W65_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBI3097381.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(590..1255)
                     /locus_tag="K0W65_004437"
     CDS             complement(590..1255)
                     /locus_tag="K0W65_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBI3097382.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          331 a          342 c          314 g          349 t
ORIGIN      
        1 ccatccctct tctccctgcg cgctacttaa gtgagtcgat tctatctgcg aaggtgcgcg
       61 aatcaaattc accggaaata aacaacaaaa aacccgccga aacgctgaga gatcacagcg
      121 atgggcgggt taagtgcggg tgcgttgagg atgcctgaca catcagaggt gggcggggat
      181 gggatcagct ccccacccgg tcactcttac ttcctggatt cgtagtctac gaagacagcg
      241 acctccgtct ggccggttcg gattcgtacc tcacagaggt ctttcctcgt taccagtgcc
      301 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
      361 atagcctgct tctccttgcc tttcggcatg taagaggcta acctacatgt gcaaagcatg
      421 aaattggcct cagattaatg ttaagcgtct tgccggacgc gtaatgttaa ctggggcttt
      481 tctctatctg ccttttggtg ttcatgcctg aagcagatag cctcaagcac ccgcagtcat
      541 tctacttacc tcgccaatat gaaatcaatc agaaaggtgc cccataaaat cactccttct
      601 cttctttacc gtagtggagt tgaccaattt tgataagagg gcgtccctga gatttgcggt
      661 gtagattggt atcgcgcaga gaatacacac agccacaata ttcctgctga tagaattttt
      721 cgcgcttgct gatttcaatc atacgggacg agccgccctg cttgcgccag ttataatccc
      781 agtacaccat gcccggataa tgcgcgacgg ctcgccgccc acagtcgtta acctgctgca
      841 tatttttcca gcgtgaaatg cccagtgaac tgctgatcac actgaaacca ttttcagcgg
      901 cgtacagcgc tgtccgctca aaacgcatgt caaaacacat ggtgcaacgg atccctcgtt
      961 cgggctccca ttccattcct ttggctcgtt caaaccagtt gtctgtgtcg taatcagcat
     1021 caataaacgg cacgccgtgt tgttcagcaa agcgaatatt ctcatcctta cgaattaaat
     1081 actctttctg aggatgaatg ttcgggttgt agaaaaagat ggtgtagtcg atttccgagg
     1141 cctgaagcgc ctccatcacc tcaccggaac atggagcaca gcaagagtgc agtagtagtt
     1201 tgtttgcccc gtttgggagc tccaatttgg gccgtttgaa atcagcaact gtcataaata
     1261 tgtttattga ggtcataaaa atatcaaaga gtgtagcatt agaacggggc tatcggaaac
     1321 agatgtgtga ctgctc
//
LOCUS       DAETQL010000277         1330 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.281, whole genome shotgun sequence.
ACCESSION   DAETQL010000277 DAETQL010000000
VERSION     DAETQL010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.281"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(41..100)
                     /locus_tag="K0W65_004438"
     CDS             complement(41..100)
                     /locus_tag="K0W65_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBI3097383.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1247)
                     /locus_tag="K0W65_004439"
     CDS             complement(339..1247)
                     /locus_tag="K0W65_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097384.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          266 a          354 c          321 g          389 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca
//
LOCUS       DAETQL010000278         1287 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.282, whole genome shotgun sequence.
ACCESSION   DAETQL010000278 DAETQL010000000
VERSION     DAETQL010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.282"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..282
                     /locus_tag="K0W65_004440"
                     /pseudo
     CDS             1..282
                     /locus_tag="K0W65_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            299..817
                     /locus_tag="K0W65_004441"
                     /pseudo
     CDS             299..817
                     /locus_tag="K0W65_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          385 a          297 c          284 g          321 t
ORIGIN      
        1 gaacaaatgg tagtgtccgc tattgccagt acacctcacc tggtgtatat ccgcactcgc
       61 gaaacatatg gaacccggcg gctccagacg gagctggcag ataacggaat tatcgtcggt
      121 cgtgacagac tggcaggtct tcgtaaggag ctgaggctgc actgtaagca gaaacgcaag
      181 ttcagggcga ccacaaactc tgaccacaat ctgccggtta cgccgaatct gctgaatcag
      241 aactttacgc ccacagcacc taatcaggtc tgggtcgcag attcagttgt ccaggccttc
      301 cgaaatcaac cgactgaagg cgctggtcgc gaaactgcag cgtatgcagt tcggtaaaag
      361 ctcagaaaaa cttcgcgcaa aaaccgaacg gtggatacag gaagcacagg agcgaatcag
      421 cgcacttcag gaggaaatgg cggaaacact gggtgagcaa tatgacccgg tactgccatc
      481 ttccctgcgc cagtcttcag cccgtaaacc gttaccagcc tcacttcccc gtgccccccg
      541 ggtcatccgg ccggaagagg aatgctgtcc ggcctgtggt ggtgaactca gtccactggg
      601 atgtgatgtg tcagagcagc tggagcttat cagcagcgcc tttaaggtta tcgaaaaaca
      661 acgccctaaa ctggcctgtc gccggtgcga ccatatcgtg caggcaccgg tgccttcaaa
      721 acccattgca cgcagttatg ccggagcggg gcttctggcc catgttgtta ccggaaagta
      781 tgcagaccat ctgccattat accgccaaag tgatctacta ttccacacag ctatttagct
      841 ctctttttgt tgagctccca tgattctttc atttattgtt actaattatt ttatgttatc
      901 atgatgttag aacacgctct gctctgtgtc tctattttat ctatcatatt ctgaggatta
      961 actacataac aaggcgcaag atatatatac aaaaacaaat aataccgttg caataattcc
     1021 ttttagtaaa ggaggtgtga ttaatttcag gggatttcag agtaaaaatt gtacaacata
     1081 ttaaaatatg ttcatttcgt tataatacac acaaggaaat attaagttag ataatatatt
     1141 ctaaataacc atagataaaa acgcacataa tggggaatat tttatgaaaa tacctgaagc
     1201 agtaaatcat attaatgttc agaataatat tgatcttgtt gatggaaaaa cgaatccgaa
     1261 taaagcaaca aaagcattac aaaaaat
//
LOCUS       DAETQL010000279         1279 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.283, whole genome shotgun sequence.
ACCESSION   DAETQL010000279 DAETQL010000000
VERSION     DAETQL010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.283"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="K0W65_004442"
     CDS             complement(<1..835)
                     /gene="ipaH4.5"
                     /locus_tag="K0W65_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HBI3097385.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          358 a          230 c          287 g          404 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga acgtgagtcg
       61 acaatggatt ccctgaaatg ttaacattcg tacaaatggg caatgtgatt atattttcgg
      121 gtaatgaagt tagtaggttg ttggaaacat ccagtgccaa tagttcttgc ggtaatctgg
      181 ataatctgcg tagttgattt tcacctacct tcaaatattt taagctctga ggaaattctg
      241 gtagttcctg tagtctgttg ccggaaacaa agagttcttg tagtctatga ggcaattcag
      301 gcaagacagt aaggcgatta ccttcaatac gtaatattgt tagcgttgat ggaagctctg
      361 gtaacgtctc cagactatta aaacgtgccg agagtgattg taaatatggt ggtaatgatg
      421 gcagacatga caaatttcga ttgaatgaaa tattaagctt aatcagttga gatggaagtg
      481 tcgggagtat attcaagttg ttaccattta catgaagttc tgtcaaaagc ggagaatttt
      541 caggtaggga gattaactca ttgttggaaa tattaagttc acgtatatgc aaagggagtg
      601 gtggtaaaga acgtagtttc agtaagctta aattgagaac aggctcccgg gtatccagac
      661 atattttcaa taatcgaacc gcctggatgc gattttctgt tgttgttcct tccctagccc
      721 agtcatccca gatgcggtgg taatctctgg tacactgttc ttctaccgat aaacgagata
      781 tacatgataa gccacaaagg gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt
      841 aacccctcac tattcatatc ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa
      901 aaacaatcct agagctttat cggcgttttc attccggctg gaggaatatg ttcctgtctt
      961 tccagtaacc gtcgattgtt atatcagtct acccatgtta atgtgaccag ttctactttc
     1021 atccagtttg gattgatctt tatcccctat attttcaaac aatttttatc atttaactca
     1081 ttactgtctc gatattccca tagcaaacga tctggttggt tatcccctca aaaaagtcag
     1141 ttgcacgccg taaatccctg ccggtattga gcgaggattt acgggttgtt gttaaggtcc
     1201 gtaatcgggt cacagttttt ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt
     1261 gattatgaat ggtgcagtc
//
LOCUS       DAETQL010000280         1275 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.284, whole genome shotgun sequence.
ACCESSION   DAETQL010000280 DAETQL010000000
VERSION     DAETQL010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.284"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            34..435
                     /locus_tag="K0W65_004443"
     CDS             34..435
                     /locus_tag="K0W65_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBI3097386.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="K0W65_004444"
     CDS             447..818
                     /locus_tag="K0W65_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3097387.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1275
                     /locus_tag="K0W65_004445"
     CDS             805..>1275
                     /locus_tag="K0W65_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3097388.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          285 c          396 g          291 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccg
//
LOCUS       DAETQL010000281         1274 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.285, whole genome shotgun sequence.
ACCESSION   DAETQL010000281 DAETQL010000000
VERSION     DAETQL010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.285"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..143
                     /locus_tag="K0W65_004446"
     CDS             <1..143
                     /locus_tag="K0W65_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3097389.1"
                     /translation="AEVELATLTWVDWYNNRRLPERLGHIPPAEAEKAYYASIGNDDL
                     SA"
     gene            127..486
                     /locus_tag="K0W65_004447"
     CDS             127..486
                     /locus_tag="K0W65_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097390.1"
                     /translation="MICQPEFTDKTLSRKPGAVQKVNETDAEYLVRKFDELEAKCAAQ
                     ENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLEN
                     VNITHWAVMPKAPEAGA"
     gene            486..791
                     /locus_tag="K0W65_004448"
     CDS             486..791
                     /locus_tag="K0W65_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097391.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            791..1141
                     /locus_tag="K0W65_004449"
     CDS             791..1141
                     /locus_tag="K0W65_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3097392.1"
                     /translation="MEPYSLTLDEACHFLKISRPTAINWIRTGRLQATRKDPTKNKSP
                     YLTTRQACIAALQSPLHTVQVSAGDGITEERKCHSSAEVKYGTPVSHCRTVKDLNSLL
                     EQRTKGRRQNSMTS"
BASE COUNT          406 a          278 c          323 g          267 t
ORIGIN      
        1 gtgcagaagt ggaactggcc acactaacgt gggtggactg gtataacaat cgacgattgc
       61 cggaaaggct gggccacatc cctccggcag aagcagaaaa agcttattat gcttccatcg
      121 gaaacgatga tctgtcagcc tgagttcaca gataaaacac tctccaggaa acccggggcg
      181 gttcagaaag taaatgaaac agatgctgaa tacctggtgc gtaaattcga tgagcttgaa
      241 gctaaatgtg cagcacagga aaacaaagta ataccagtgt caactgaact gccaccagca
      301 aatgaaagtg ttttgttatt cgatgctaac ggagaaggct ggctaattgg ctggcgttct
      361 ctctggtaca cctggggaca aaaagaaacc ggagaatggc agtggacatt tcaggtcggg
      421 gaccttgaaa acgtcaatat cactcactgg gcagtaatgc caaaagcacc ggaggctgga
      481 gcataatgac cacttttacc gacaaagaac tgattaaaga aattaaagag cgtatcagca
      541 gccttgacgt gcgagacgat attgagcgcc gtgcttatga aatcgcactc ctatctctgg
      601 aagtagaacc agatgaacgc gaagcttatg aattattcat ggaaaagcgt tttggtgact
      661 tagtagatcg tcggagagca aaaaacggcg ataacgaata catggcatgg gatatgactc
      721 tcggttggat cgtctggcag caacgagctg gtatccattt ctcaacaatg tcacagcaag
      781 aggtgaaata atggagccat acagcctcac actcgatgag gcctgtcatt ttctcaagat
      841 atccagaccg actgccatta actggatacg cacagggcgt cttcaggcaa cacgcaaaga
      901 tcccactaag aataaatctc cttacctcac aacacgacaa gcctgcattg cggctcttca
      961 gtctccgctg catactgtcc aggtgagcgc gggtgatggc ataacagagg aaagaaaatg
     1021 tcactcttcc gcagaggtga aatatggtac gccagtttca cattgccgaa cggtaaaaga
     1081 tttaaacagt ctcttggaac aaaggacaaa aggcaggcga cagaactcca tgacaagcta
     1141 aaggctgaag catggcgggt cagcaaactt ggtgaaatac ctgatataac gttcgaggaa
     1201 gcgtgtgtca ggtggcttga agagaaagca cataaaaaat cactggacga tgacaaaagc
     1261 cggatcggat tctg
//
LOCUS       DAETQL010000282         1267 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.286, whole genome shotgun sequence.
ACCESSION   DAETQL010000282 DAETQL010000000
VERSION     DAETQL010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.286"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..787)
                     /gene="ipaH3"
                     /locus_tag="K0W65_004450"
     CDS             complement(<1..787)
                     /gene="ipaH3"
                     /locus_tag="K0W65_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HBI3097393.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNRLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLSQQTAQPDYHGP"
BASE COUNT          366 a          212 c          311 g          378 t
ORIGIN      
        1 gtgggccgtg gtagtccggt tgggcggttt gttgcgacag agactccctg atccgtgagg
       61 acagaggatt gtcttcgagg atgatagtgc aggtcggatc aaggctaagt atattttccg
      121 gaatgtgtgt gatgcgattc tcgcggcacc ggaaaaatat ctcggtttcc tctgagtgat
      181 gatttcttac aggtacggct ggtaggcttt ccagaagatt agtacttaca tcgagcgctt
      241 ccagtgattc aggtaactca gggagaaatg tcagctggtt atttcttact gagagcactt
      301 ccagcgatgt aggtaattca ggaagcatgg ttagctgatt gttatctgca ttaatatatt
      361 ccagcaatgc aggcaattca ggaagcatgg ttagttggtt gttatctaca tcaagatgtt
      421 tcagagatgc gggtaattca ggaagtgttg acaggcgatt gtcacaggcg tcaaggtatt
      481 ccagcgatgc tggcaattct ggtaatgata ttagggcatt ctgagtaatt tccagaacag
      541 tgatttgagg tggtaagttg tcaggtagcg aggacagatt taaacgattc agttgaagct
      601 cactgaactg attgatgaga cattctttaa gtagggagac cgcttcattc cgattttcac
      661 cggggagcgc ttgtttttcc catttatccc atgctgaaaa gtaatcagca tatgtaccgg
      721 aaatagtgtt ataaaaagaa ttttgggaca atgaaaagtt attatttatc ggtaacatga
      781 tggacattca gttaaagccg atggacaaat ggctttgtta aaagaaataa gtaggagttt
      841 ttatgaagtt cattccatga aatgaacttc agcacttttt tattataaaa ggacaaacaa
      901 aattggctgt aaaaaaacga tactgtttca gttaactgtg aatagcttgc ttgcaccgta
      961 ttgttcttgt ttttcggtta gttaaacatc gtacttcata tttgaacgtt ctgccggaat
     1021 gcattatcaa tagaggtaaa gtcgcaaccc caaatcgtaa aggaaaccgt agcacgtcgt
     1081 atgcaagaac gtgccacggc tggctgatgg atgttcgata gcgcgagttt gaatgaaaat
     1141 cagccggaga tgattttaca taattgctac ggaattattc aatacaggaa ttgcttgcgt
     1201 ctgcatggat tgacctgaaa tattcccgaa aatttctcta aaaaactcga aaaaaatggt
     1261 aactggt
//
LOCUS       DAETQL010000283         1244 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.287, whole genome shotgun sequence.
ACCESSION   DAETQL010000283 DAETQL010000000
VERSION     DAETQL010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.287"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            55..>1244
                     /locus_tag="K0W65_004451"
     CDS             55..>1244
                     /locus_tag="K0W65_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBI3097394.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFAV
                     AADAT"
BASE COUNT          368 a          303 c          213 g          360 t
ORIGIN      
        1 cattaggaga gatacattaa atacaattta ttcacaatct aattccatat ccatatgcga
       61 gaaattaata tgctcagaaa catttcatcc tgtttatttc cacatatcag cacaattaca
      121 tcccccaacc attatttgtc cgaatgggat gattgggaga aacaggggtt accggaagaa
      181 cagcgtactg aggcggtaag aagacttcgt gcatgtctta cctctaaggg gcataaactg
      241 gacctgcgag ccttggcgct ttcctcgcta cctgtactcc ctgcttgcat taaaaagctt
      301 gatgtgagct gtaataaatt aaccatcctt actgatttac ctgaaaatat taaagaactt
      361 attgcaagag ataatttctt aacacatata tctgcattac cacattatct aataactttg
      421 gatgtgtccg aaaatcaatt agagaatctg ccgttattac cagacaccat caaatcacta
      481 agcgcagagt ataataggtt atccacactg ccttcattac ccttgaattt aaaaaaactt
      541 gaggttagga acaacgaact gcaaactctt ccatctctgc cttctaatct taagatactt
      601 aaggttgcgc acaaccatct tactgaactg ccccctttac ctaggagact gcaacttctt
      661 tttgcatata gcaatagatt aagcaactta ccaaacatcc aagaaaatat tatcatgaga
      721 agattttttt attttgaaaa caaccaaata actacaatcc cgacaaatct ttttcgttta
      781 gatcctcata taactattga gattgcaaat aaccccttat cagatcaaac tctgctattc
      841 ttaatacagc aaacttcggt tccaaatttt aacgggcctc agtttcgtat ttccctgtca
      901 gaccaaaaca gactgttttt acgccagatg ttgccgcaaa atttacattc gcgccatatc
      961 agagtcatca ctgaaggggg gcagaacttt cagatccccc ctcttcccga aactgtggca
     1021 gcctggtttc ctgaagcaga tcgtcgggag gtttctacac aatggacttc tttttccacc
     1081 gaggagaatt cccgggcatt ctccgcgttc cttgaccgcc tttccgatac cgtctctgca
     1141 cgcaatacct ccggattccg tgaacaggtc gctgcatggc tggaaaaact cagtgcctct
     1201 gcggagcttc gacagcagtc tttcgctgtt gctgctgatg ccac
//
LOCUS       DAETQL010000284         1219 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.288, whole genome shotgun sequence.
ACCESSION   DAETQL010000284 DAETQL010000000
VERSION     DAETQL010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.288"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            296..>1219
                     /locus_tag="K0W65_004452"
     CDS             296..>1219
                     /locus_tag="K0W65_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBI3097395.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEHSLADAVTAWFPENKQSDVSQIWHAF"
BASE COUNT          389 a          270 c          235 g          325 t
ORIGIN      
        1 gagcaggtac cggcatccac gttacagacg gtcccggcaa aagtgcctga cctgacccct
       61 attagcggtt accagcgccc caccagaccc ggtgacatca ccccccggat catggacgac
      121 tgggggcagt aatcatgccg ggtctggctg gctaacaccc cattataagg ggtacgaata
      181 aagtaacggg ggaagcaata cctggagaaa gagtacaaag caaactgtat taaacttaat
      241 aagttatatt gtatttcact agagttctta tcgttgataa gaaattctgg ttataatgat
      301 taaatcaacg aatatacagg taatcggttc tggtattatg catcaaataa acaacataca
      361 ctcgttaact ctattttctt tgcctgtgag tttgagtcca tcttgtaatg aatattattt
      421 aaaagtctgg agtgaatggg aaaagaacgg taccccaggc gagcaacgca atatcgcctt
      481 caataggcta aaaatatgtt tgcaaaatca aggggcggaa ttaaatctat ctgagttaga
      541 tttaaaaaca ttacctgatt taccgcctca gataacaaca ctggaaataa gaaaaaacct
      601 attaacatat ctccctgatt tcccaccaat gcttaaggta atacatgctc aatttaatca
      661 actggaaagc ttacctgcct tacccgagac gttagaagag cttaatgtgg gtgataacaa
      721 gataaaagaa ttaccatatc ttcctgaaac tctaactcat ttacggatcc ataataaccg
      781 attgcatatt ctgccactat tgccaccgga gctaaaatta ctgatagttt ctggaaacag
      841 attagacagc atccccccct ttccagataa gcttgaaggg ctggctctgg ctaataattt
      901 tatagaacaa ctaccggaat taccttttag tatgaacagg gctgtgctaa tgaataataa
      961 tctgacaaca cttccggaaa gtgtcctgag attagctcag aatgccttcg taaatgttgc
     1021 aggtaatcca ttgtctggcc ataccatgcg tacactacaa caaataacca ccggaccaga
     1081 ttattctggt cctcgaatat ttttctctat gggaaattct gccacaattt ccgctccaga
     1141 acactccctg gctgatgccg tgacagcatg gttcccggaa aacaaacaat ctgatgtatc
     1201 acagatatgg catgctttt
//
LOCUS       DAETQL010000285         1214 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.289, whole genome shotgun sequence.
ACCESSION   DAETQL010000285 DAETQL010000000
VERSION     DAETQL010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.289"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(130..273)
                     /locus_tag="K0W65_004453"
     CDS             complement(130..273)
                     /locus_tag="K0W65_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097396.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            512..>1214
                     /locus_tag="K0W65_004454"
     CDS             512..>1214
                     /locus_tag="K0W65_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBI3097397.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQWD"
BASE COUNT          398 a          243 c          243 g          330 t
ORIGIN      
        1 ataaagtccg gtaatggaga ttgttaatac gatggtgcac gatgcatcgt cagtgcccgg
       61 cccggaggat gccccgtagc tacatacgga tcagggttgg caatatcgaa tggcaggcta
      121 taggcaaagt taaagcccca tggagtagca caaaatatgc cgcacaaagg aaacggtctg
      181 aataacgcag tgatggagaa cttcttcggc acactgcaaa atcgcatcgt ggtcgatcgc
      241 taattcgtcg tgaatcactg aacgatgttc catgcgaaca gatattcgat catatcgaaa
      301 tattaaacat tacccctccc cacattgggc aaaaaaaaac aaaaaacgat caattagtat
      361 tgattcacat aatctctcat cattaatgag aacattgtca catatttaca atatagaact
      421 gttactttta gcaagctgtt agtaaccaac cgttactaat aattttaatt cttaatactt
      481 tgttcattca ataaatgagt tgagagaata tatgaataaa atatactccc ttaaatatag
      541 tgctgccact ggcggactca ttgctgtttc tgaattagcg aaaagagttt ctggtaaaac
      601 aaaccgaaaa cttgtagcaa caatgttgtc tctggctgtt gcaggtacag taaatgcagc
      661 aaatattgat atatcaaatg tatgggcgag agactatctt gatcttgcac aaaataaagg
      721 tattttccag cccggagcaa cagacgtaac aatcacttta aaaaacggag ataaattctc
      781 tttccataat ctctcaattc cggatttttc tggtgcagca gcgagtggcg cagctaccgc
      841 aataggaggt tcttatagtg ttactgttgc acataacaaa aagaaccctc aggccgcaga
      901 aactcaggtt tacgctcagt cttcttacaa ggttgttgac agaagaaatt ccaatgattt
      961 tgagattcag aggttaaata aatttgttgt ggaaacagta ggtgccaccc cggcagagac
     1021 caaccctaca acatattctg atgcattaga acgctacggt atagtcactt ctgacggttc
     1081 aaaaaaaatc ataggttttc gtgctggctc tggaggaaca tcatttatta atggtgaatc
     1141 caaaatctca acaaattcag catatagcca tgacctgtta agtgctagtc tatttgaggt
     1201 cacccaatgg gact
//
LOCUS       DAETQL010000286         1201 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.290, whole genome shotgun sequence.
ACCESSION   DAETQL010000286 DAETQL010000000
VERSION     DAETQL010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.290"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            115..1164
                     /gene="cadB"
                     /locus_tag="K0W65_004455"
                     /pseudo
     CDS             115..1164
                     /gene="cadB"
                     /locus_tag="K0W65_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
BASE COUNT          256 a          264 c          309 g          372 t
ORIGIN      
        1 tttaggatta atcctttttt cgtgagtaat cttatcgcca gtttggtctg gtcaggaaat
       61 agttatacat catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt
      121 tctgccaaga agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg
      181 agcggtattg cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt
      241 tggattatct ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca
      301 aaaaacccgc aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt
      361 tttcagacag gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt
      421 accgctgtat cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt
      481 atcgcctgta ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacctgg
      541 gtaagccgtt taaccactat tggtctggtg ctggttctta ttcctgtggt gatgactgct
      601 attgttggct ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat
      661 accactgatg gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt
      721 gttgaatccg cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg
      781 gcaaccatgc tgggtactgg tttagcaggt attgtttata tcgctgcgac tcaggtgctt
      841 tccggtatgt atccgtcttc tgtaatggcg gcttccggtg ctccgtttgc aatcagtgct
      901 tcaactatcc tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc
      961 ctgacttctc tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac
     1021 gacggtaact tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt
     1081 ctgctgctgg ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct
     1141 gccggtggta aagcatctga cctgtgaggt gtactggcaa tagcggacac taccatttgt
     1201 t
//
LOCUS       DAETQL010000287         1173 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.291, whole genome shotgun sequence.
ACCESSION   DAETQL010000287 DAETQL010000000
VERSION     DAETQL010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1173
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.291"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(212..837)
                     /locus_tag="K0W65_004456"
                     /pseudo
     CDS             complement(212..837)
                     /locus_tag="K0W65_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(767..>1173)
                     /locus_tag="K0W65_004457"
     CDS             complement(767..>1173)
                     /locus_tag="K0W65_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3097398.1"
                     /translation="YWPISRLTGWVKINITTEDIIYRIVASSVRNRWGDPDIGGLIIA
                     AYQGEADGDKVIRLVRGQSYRGSRLGPVGISVPGTPTGTYIVSPQFFITGCSEHSLPG
                     SYCALSGVPDAHVSGAMPGLFIRTSRGMHRGN"
BASE COUNT          253 a          355 c          273 g          292 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaccac agaccggact
       61 gactccggaa aaacggaggc ccgcccccgg gcctcccctg attcatccgt ttccctgttc
      121 agcctgacag cacgccccgg ctgccggatg acagactccg cttcggtaag caaagcggtc
      181 ttctgtgatt ccgccagttg cggcttattc attactcaac gtcaaacgcc cgaattgaag
      241 ccaaatcatc cagaccgctc agctcctctt tcatttcacg ctgacggcga taaatctcat
      301 cgttgcgatc gacctgcgcc tgcaccattg ctgccgccag ttcttccagt tccggcatcg
      361 acagtttcac ctgctgatta tcggcatcgc tccacgccat atgtgtttgt gctgtgacag
      421 attttgccag catgactacc ggggacaggc ggcccagtga gtcggggcca gcattccaga
      481 tacgaccgtt ccattcaaac gtgaacggct tcgcctcctg ttctgtgcgc catgcttcaa
      541 tttcctgacg tctggcctct ctggccgctt ccagcatttc tggtgtcaca gtgaatgggg
      601 ctatctcacc ccatttgcca ctttgcagtt cctgccagat ttgctgaccc gtcggtgcga
      661 catcatcagc ggtggctgtg taggggactg cctggtccct gtcgtcaaaa aaaaaacgtc
      721 acagtctact gcgccacttt cggtataacg gggattaatg atttttttaa tttccacggt
      781 gcattcctct cgatgtgcga ataaaaagcc cgggcattgc gccagagaca tgtgcatccg
      841 gcaccccgga cagggcgcaa tatgaccccg gtaatgaatg ctctgaacat cccgtaatga
      901 aaaattgtgg ggatactata tacgttccgg tgggagtacc gggcactgaa atccccaccg
      961 gtcccagtcg tgagcctctg tatgactgcc ccctgacaag tctgatgact ttatcaccgt
     1021 cagcttctcc ctggtacgca gcaataatca gcccgccaat gtcagggtct ccccatctgt
     1081 tgcggacaga gctcgccacg attctgtaaa taatatcttc tgtggttata tttattttca
     1141 cccagccagt cagtctggat atgggccagt agc
//
LOCUS       DAETQL010000288         1162 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.292, whole genome shotgun sequence.
ACCESSION   DAETQL010000288 DAETQL010000000
VERSION     DAETQL010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.292"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..388
                     /locus_tag="K0W65_004458"
     CDS             <1..388
                     /locus_tag="K0W65_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097399.1"
                     /translation="LVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTACACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(389..1033)
                     /locus_tag="K0W65_004459"
     CDS             complement(389..1033)
                     /locus_tag="K0W65_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3097400.1"
                     /translation="MTPTGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSL
                     IDIVQQGFDAGVRLSGIVEKDMISVAIGPPVKLCVAATPEYFARYGKPRHPHDLLNHQ
                     CVVFRYPSGKPFHWQFAKELEIAVAGNIILDDVDAELEAVLMGAGIGYLLYEQIKEYL
                     DTGRLECVLEDWITERPGFQVYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT          295 a          264 c          279 g          324 t
ORIGIN      
        1 cctggtgctg gtggcaattc tgattatgct gccgactctc ggcatcggtg atatgaacaa
       61 tctctataaa tggctgttga atcttaactc ggtagtgatg ccgctgcgtt atttatgggt
      121 atttgttgca tttattgcag tcgttcgcct ggcgcagaaa tataaaccag aatatgtctt
      181 tattcgtaat aagccgctgg caatgaccgt cgggatttgg tgttttgcct ttaccgcctg
      241 tgcctgtttg acggggatct tcccgaaaat ggaagccttc actgcagagt ggaccttcca
      301 gttggcgctg aatgttgcaa cgccgtttgt gctggtagga ttaggactga tattcccgct
      361 gctggcgcgt aaagcgaata gcaaataatt attgtggtcg gtgacgctga ctctggcaga
      421 tctgacccgt ttttacataa tcgagaaatg cgcgcaagcc gcaggacata tactggcggt
      481 ttgggtaata gacttggaag cctggacgct ctgtgatcca gtcttccagt acacattcta
      541 aacgaccagt atcaaggtac tccttgatct gttcatagag cagatacccg ataccagcac
      601 ccatcaaaac ggcctccagt tcagcatcaa catcatccag aatgatattt cctgcgacag
      661 caatttccag ctcctttgca aattgccagt ggaaaggttt gccgctggga taacgaaaaa
      721 caacacattg atgattaagc agatcatgag gatggcgggg tttgccatat cttgcaaagt
      781 actctggcgt tgctgctaca cataatttca ctggtgggcc gatcgccacg ctgatcatat
      841 ctttttcgac gatgccacta agtcgaacgc cagcgtcaaa gccctgttgg acgatatcga
      901 taagagaatc gtcagttgtg agctctactt taatatcagg atattcacgt gtgaaaccaa
      961 ccagtaaagg cattaaaaat atacgtgcag ctacccgggc ggcattgatt ttgagcgttc
     1021 ctgtcggtgt cagacgaaaa tcattcatct catctaacat gatctgaatt tcgtcgaaag
     1081 ctgggcgcaa tcgttcatag agattagaac ccgcttccgt taacgatacg cttcgggttg
     1141 tccgattgaa taagcgaatt tt
//
LOCUS       DAETQL010000289         1144 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.293, whole genome shotgun sequence.
ACCESSION   DAETQL010000289 DAETQL010000000
VERSION     DAETQL010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.293"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..734
                     /locus_tag="K0W65_004460"
     CDS             <1..734
                     /locus_tag="K0W65_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HBI3097401.1"
                     /translation="KTDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMV
                     NKPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITF
                     TGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASG
                     VHNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDR
                     PLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(736..962)
                     /locus_tag="K0W65_004461"
                     /pseudo
     CDS             complement(736..962)
                     /locus_tag="K0W65_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          301 a          283 c          248 g          312 t
ORIGIN      
        1 agaaaaccga cagaatccat ttgaaagacg cagcgcgaat gctggaagtt tctgtaatga
       61 ctattcgtcg cgatctccat caggaagatg aacctctgcc actgacccta ctgggtggct
      121 atattgtaat ggtgaataaa cccgcgccat ccatgccagt aatccatgac gttccaaaaa
      181 atcatcgtga tgacttacct attgcaattc tggctgccgg aatggttaat gaaaatgatc
      241 tgatcttctt tgataatggc caggagatac cactcgttat aagcatgatc ccggatgcaa
      301 tcaccttcac cggcatctgt tactcacatc gcgtctttgt tgcgttgaat gaaaagccta
      361 atgtaacagc aatactttgt ggtggtacgt atcgtgccag aagtgatgct ttttacgatg
      421 ccagtaactc ttcgccatta gactctctca atccgcgaaa aatatttatt tccgccagcg
      481 gtgtgcataa tcactttggc gtcagctggt ttaaccctga agatcttgcc actaagcgta
      541 aagtgatgaa ccgtggacta cggaaaattt tgctcgcccg ccacgcgttg ttcgatgaag
      601 tggcctctgc cagcctcgca ccgatctctg catttgacgt tctgattagc gatcgtccgt
      661 taccggcaga ttatgttacg cactgccaga atggttctgt aaagatcatt acacctgatt
      721 cagaagacga atgacttact gaaaaaacac cacaatcttg ttaaacatcg tcgggttgga
      781 ctgattacgt tgcactttca ccacatattc cagcttatct atttggctta tcacctactc
      841 cagacgctgg tcatccttga ccagtagcca gatatggctt ttgtcgcagt cctgaatcgg
      901 caggcagaga atgtcttcaa cgttaagagc gcgacgggca aaagcccaca aaggtgagtc
      961 attacacccg gatggtaaac aggcacttcc ttgttgtaga agccgttata ttgtccataa
     1021 tgtctgggtt tttaatgcat actgggtaac aaccggacga atcttcgaat atcactggcg
     1081 ctgccagccg gtttgaattc atctcacaac cctgcataga gcgaatctcc tgcctgcacg
     1141 tcac
//
LOCUS       DAETQL010000290         1129 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.294, whole genome shotgun sequence.
ACCESSION   DAETQL010000290 DAETQL010000000
VERSION     DAETQL010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.294"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(202..825)
                     /locus_tag="K0W65_004462"
     CDS             complement(202..825)
                     /locus_tag="K0W65_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBI3097402.1"
                     /translation="MHMSLAQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFD
                     DRDQAVPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAW
                     RTEQEAKPFTFEWNGRTWNADASSVARLSPVVMLAKSVAAQTHMVWSDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSSLDDLASIRAFDVK"
     gene            complement(776..>1129)
                     /locus_tag="K0W65_004463"
     CDS             complement(776..>1129)
                     /locus_tag="K0W65_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBI3097403.1"
                     /translation="VKINITTEDIIYRIVASSVRNRWGDPDIGGLIIAAYQGEADGDK
                     VIRLVRGQSYRGSRLGPVGISVPGTPTGTYIASPQFFITGCSEHSLPGSYCALSGVPD
                     AHVSGAMPGLFIRTS"
BASE COUNT          241 a          350 c          258 g          280 t
ORIGIN      
        1 ctcacaactg caccattcat aatcacatcg cataaaccac agaccggact gactccggaa
       61 aaacagaggc ccgcccccgg gcctccccgg attcatccgt ttctctgttc agcctgaccg
      121 cacaccccgg cggccggatg acagactccg cctcggtaag caaagcggtc ttctgtgatt
      181 ccgccagttg cggcttattc attacttaac gtcaaacgcc cgaattgaag ccaaatcatc
      241 cagactgctc agctcctctt tcatctcccg ctgacggcga taaatctcat cgttgcgatc
      301 gacctgcgcc tgcaccattg ctgccgccag ttcttccagt tccggcatcg acagtttcac
      361 ctgctgatta tccgcatcac tccacaccat atgtgtttgt gccgcgacag attttgccag
      421 catgaccacc ggggacaggc gggccactga cgaggcgtca gcgttccacg tgcggccgtt
      481 ccattcaaac gtgaacggct tcgcctcctg ttctgtgcgc catgcttcaa tttcctgacg
      541 tctggcctct ctggccgctt ccagcatttc tggtgtcaca gtgaatgggg ctatctcacc
      601 ccatttgcca ctttgcagtt cctgccagat ttgctgaccc gtcggtgcaa catcatcagc
      661 ggtggctgtg tagggaactg cctggtccct gtcgtcaaaa aaaacgtcac agtctactgc
      721 gccactttcg gtataacggg gattaatgat ttttttaatt tccacggtgc attcctcacg
      781 atgtgcgaat aaaaagcccg ggcattgcgc cagagacatg tgcatccggc accccggaca
      841 gggcgcaata tgaccccggt aatgaatgct ctgaacatcc cgtaatgaaa aattgtgggg
      901 atgctatata cgttccggtg ggagtaccgg gcactgaaat ccccaccggt cccagtcgtg
      961 agcctctgta tgactgcccc ctgacaagtc tgatgacttt atcaccgtca gcttctccct
     1021 ggtacgcagc aataatcagc ccgccaatgt cagggtctcc ccatctgttg cggacagagc
     1081 tcgccacgat tctgtaaata atatcttctg tggttatatt tattttcac
//
LOCUS       DAETQL010000291         1109 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.295, whole genome shotgun sequence.
ACCESSION   DAETQL010000291 DAETQL010000000
VERSION     DAETQL010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.295"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..1019
                     /locus_tag="K0W65_004464"
                     /pseudo
     CDS             1..1019
                     /locus_tag="K0W65_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          312 a          271 c          258 g          268 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaac
      541 cttttggccc ggagcaacga caaggattat ggctctgtca cgttatcgaa ccggatcgct
      601 gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg cggcatttac gccggacatg
      661 acaatcattt ctatgggcat cggaaaatcc tcaaaccaga acatttagac tggcaagaat
      721 atgcattatt gctgctcaat agcatgccgg aaaaaacagc tgagcattac cgcaataaaa
      781 tcgccattta tttgcactgg tatcagaaaa aaggcatcga ggttccacaa acccagcaag
      841 gggacattgg cgcgaaagat atcccctcct ggcggcggat atgcaaagtt ttactcaata
      901 acgattactg gtgtcgggca ttatcattta gtcctacgaa agcgaagaac tatcagcgtt
      961 ataacgaacg gataaaagga aaacgtcagg aatgggggat actatgcaac aacgattaac
     1021 gcaggattta acgcaatttc tcgccagctt gccggaagat gatcgcatca aagcgattaa
     1081 tgaaatccgc atggcgatcc atcaggtta
//
LOCUS       DAETQL010000292         1093 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.296, whole genome shotgun sequence.
ACCESSION   DAETQL010000292 DAETQL010000000
VERSION     DAETQL010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.296"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(22..825)
                     /gene="dkgB"
                     /locus_tag="K0W65_004465"
     CDS             complement(22..825)
                     /gene="dkgB"
                     /locus_tag="K0W65_004465"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBI3097404.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(988..1064)
                     /locus_tag="K0W65_004466"
     tRNA            complement(988..1064)
                     /locus_tag="K0W65_004466"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1028..1030),aa:Asp,seq:gtc)
BASE COUNT          285 a          256 c          249 g          303 t
ORIGIN      
        1 cggaggagct gtcagagagg cttaatccca ttcaggagcc agaccttccg ggctaaccag
       61 gcggtcgttg caatccagtg cggcgatcgc ttttttatct tcggcatcaa gctgtaaatt
      121 ttgtgcctta agattacttt ccaggttttt acgtttagta gaagaaggaa ttactgagta
      181 accttccccc atagcccacg ccagaatcac ttgtgccgga atcgcattgt gtttagctgc
      241 aatacgagca ataacctcat ctttcagtgc cttaccataa gccagcgtca tataggaagt
      301 gatatgaata ccgtgctgtt tagcccaggc aaccacttta cggttttgca gataaggaga
      361 gagttcaatc tggttggtag cgatgttttc agcgccaaca gcagcaatca ctttttccat
      421 caatgggatc gtgaagttgg aaataccgat ctcacgcgtc agcccttgtt ttttggcttc
      481 cagcagcgcc tgcataaact cttcaacaga gacttcatcg tttggtgacg gccagtggat
      541 tagcgtcaga tcaacataat cggtacgcaa tttttgcagg ctctctttca gactcgggat
      601 caatttgtct ttgctgagat tttcaatcca gattttagtg gtgatgtaga gttcatgacg
      661 tggcacgcca ctttctgcaa tcgcctgacc tactgcggct tcgttatcat agatttgtgc
      721 ggtatcaatt gcgcgataac caagttcaag cgccgttttc acagatgaaa taacaacgtc
      781 gtctttcaga cggaaagtac ctaaaccaaa tgcagggata gccataatat gcctctttta
      841 atgctatttt cgttaactga ggtgattatg ggcttcagac tgttgcggga aaagagcgta
      901 aaacgcagag gatttttgcg attctagcaa taatagatat ccagacgcaa aaaaacatcg
      961 agcgttaact cgaggcttct taataagtgg cggaacggac gggactcgaa cccgcgaccc
     1021 cctgcgtgac aggcaggtat tctaaccgac tgaactaccg ctccaccgaa ttcttttaca
     1081 accaccggtt tta
//
LOCUS       DAETQL010000293         1076 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.297, whole genome shotgun sequence.
ACCESSION   DAETQL010000293 DAETQL010000000
VERSION     DAETQL010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.297"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(348..1043)
                     /gene="aqpZ"
                     /locus_tag="K0W65_004467"
     CDS             complement(348..1043)
                     /gene="aqpZ"
                     /locus_tag="K0W65_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HBI3097405.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
BASE COUNT          304 a          322 c          252 g          198 t
ORIGIN      
        1 gatttttaat cctatctgag tgtttagcat tgttgcgctc ctttcatgtg caatgccata
       61 taaagccact ggcggaggga gcgcgcaggg ggcagccagt cgccgccgcc ccctgcaccc
      121 ccgggctctg gcgaacaaaa tcgccgctgc gcggtacctt cagcttatcc ccttcggcaa
      181 ccgggtcggg cgcgaggtaa cgtcctgtaa gacgcgccct cagcccacat ccatgtgggc
      241 tgccccggcc ttcggggaac gcttcagcga ttttgacgcc accaataccc gtgcggtttg
      301 taatgtcgaa gataaaacaa ccagggcaga taaaatgcca atgctaatta atcacgcttt
      361 tccagcagag tccggtaaat cagaccaccg ataatgccgc cgacaattgg caccacccag
      421 aagaaccaca gttgttctaa tgcccagccg ccctggaaga tagcaaccgc ggtgctgcgc
      481 gccgggttaa cagaagtgtt agtcaccgga atactgatta agtgaatcag ggttaaggcc
      541 agaccaatag cgatcggcgc aaaacctgcc ggagcgaatt tgtcggttgc accgtggatc
      601 actaacagga agcccgcact caataccagt tcaactacca gcgcggaaag catggaataa
      661 ccgcctggtg aatgctcgcc ataaccgtta gaagcaaaac cgctggctgc cgcgtcaaaa
      721 cccgttttac cactggcaat taaatacagc agcgccgctg caacaatacc gccgacaacc
      781 tgggcaatta cgtagccaac gacttctttt gccggaaaac gtccgccagc ccataaacca
      841 atagtgaccg ccgggttaaa atgaccacca gaaatatgac caacagcgaa ggccatcgtc
      901 agaacggtca aaccaaatgc cagcgccacg ccggcaaaac caatgcctaa ttccgggaag
      961 cctgcggcca gtacagcact accacagcca ccaaaaacaa gccagaaagt accaaaacat
     1021 tcagctgcta attttctgaa catatccacc tcaattaaaa attgaccctg tgaaaa
//
LOCUS       DAETQL010000294         1071 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.298, whole genome shotgun sequence.
ACCESSION   DAETQL010000294 DAETQL010000000
VERSION     DAETQL010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.298"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            21..563
                     /locus_tag="K0W65_004468"
     CDS             21..563
                     /locus_tag="K0W65_004468"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097406.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
     gene            790..>1071
                     /locus_tag="K0W65_004469"
     CDS             790..>1071
                     /locus_tag="K0W65_004469"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HBI3097407.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          316 a          202 c          257 g          296 t
ORIGIN      
        1 ataacaccaa aggcgatgcc atgattggcg tcaagcagcc tttaccaaaa gcagttttaa
       61 gaactcagca tgacaaaaat aaagaagcaa taagtatcct ggattttggt gttattgatg
      121 atggtgtgac agataattac caggcaatac aaaatgctat agatgccgtt gcttcactac
      181 cctccggcgg ggagctgttt atccctgcga gcaaccaagc ggtggggtat attgttggat
      241 ccactttgct tattcctggc ggtgttaaca tcagaggagt tggtaaggca tcgcaactcc
      301 gagcaaaaag cggacttaca ggatctgtgt taaggctgtc ttatgattca gacactatcg
      361 gccgttatct gagaaatata cgagtaactg gtaataacac ctgcaatggt attgacacaa
      421 acattacagc agaagactct gtcatcagac aggtttatgg ctgggtattt gataatgtaa
      481 tggtgaatga agttgaaacc gcattattta atgcaagggc tctggcactc aaaatttata
      541 gcatgtcagg ctggaacctg tagagtcggt cttcactttt taggccagtg cgtaagtgtt
      601 agtgtcagct cctgccattt cagcagagga aattattctg ctgatgaaag ctttggcatc
      661 aggattcagc ctcaaacgta tgcgtggtcg tcagaggcag ttaggtcaga agcaataatt
      721 ttagacagtg agaccatgtg cattggtttt aaaaatgccg tctatgttca tgattgcctt
      781 gatttgcata tggaacaact ggatttagat tattgcggct caacaggcgt ggttatagag
      841 aatgtaaacg gaggattttc tttctcaaac tcatggatag cagcagatgc cgatggcact
      901 gaacaattta cggggatata ttttagaacg ccggcctcaa cgcagtcaca taaaattgtc
      961 agtggtgttc atatcaacac tgcaaacaaa aacacggctg caaacaatca aagtatagcg
     1021 atagaacagt cggcgatctt cgtctttgta agtggttgta cgttaactgg t
//
LOCUS       DAETQL010000295         1026 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.299, whole genome shotgun sequence.
ACCESSION   DAETQL010000295 DAETQL010000000
VERSION     DAETQL010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.299"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(56..196)
                     /locus_tag="K0W65_004470"
     CDS             complement(56..196)
                     /locus_tag="K0W65_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097408.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            335..1006
                     /gene="queE"
                     /locus_tag="K0W65_004471"
     CDS             335..1006
                     /gene="queE"
                     /locus_tag="K0W65_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBI3097409.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          300 a          205 c          239 g          282 t
ORIGIN      
        1 aaaggaacat acgaaagtga acattagtat gaaatagcgc attataaatt tctcattaaa
       61 tctggataaa taaattgacg gcggcatcgg taattttaga ttcgttatca ttaaaaacta
      121 atacagaaaa aataaatata attttggtga atgttttgac atgattaaat ccattttctt
      181 tattttcttc tgacataata cgttactccc tttaattatt aacgatatgt ttcgtatttt
      241 tctttaaatc tgcatgtaac agaatgttaa taagacatga ttgttgcggc aggatctgca
      301 ataattaaca attatccact tcacagagaa tgctatgcag tacccgatta acgagatgtt
      361 ccagaccctg caaggtgagg gttactttac cggcgttccc gccattttta ttcgtttaca
      421 gggatgcccg gttggctgtg cctggtgcga caccaaacac acctgggaaa agcttgagga
      481 tcgggaagtc tcccttttca gcattctggc gaagaccaaa gagagtgata agtggggggc
      541 ggcgagcagt gaagacttgc tggctgtcat tggtcgtcag ggatataccg cgcggcatgt
      601 ggtgattacg ggtggtgagc cttgcattca tgatttgctg ccactgactg acctgctcga
      661 aaagaacggt tttagctgcc agatcgaaac cagtggaact catgaagtac gctgcacacc
      721 gaatacctgg gttaccgtat cgccaaagct gaacatgcgc ggcggctatg aagtgttgtc
      781 acaggcactg gagcgagcca acgaaatcaa gcatccagtg gggcgcgtac gcgatattga
      841 agcactggat gaactactgg caacgctgac cgatgataaa ccgcgagtca ttgcactgca
      901 gccgattagc caaaaggatg atgccacacg attgtgcatt gaaacctgta ttgcgcgtaa
      961 ttggcgtttg tcgatgcaaa cacataaata tttaaatatt gcctgattac acatttataa
     1021 gcgtta
//
LOCUS       DAETQL010000296          957 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.300, whole genome shotgun sequence.
ACCESSION   DAETQL010000296 DAETQL010000000
VERSION     DAETQL010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.300"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..133)
                     /locus_tag="K0W65_004472"
     CDS             complement(<1..133)
                     /locus_tag="K0W65_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3097410.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     "
     gene            199..957
                     /locus_tag="K0W65_004473"
                     /pseudo
     CDS             199..957
                     /locus_tag="K0W65_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          259 a          202 c          230 g          266 t
ORIGIN      
        1 tctttatcca ggcataaagg ctgtgggtgg tgatatcgag acgtgttgca acgctggcaa
       61 cagaataacc gcgatcaaca acctgtttga ctgcttcagt tttaaactct tcgggataac
      121 gcttaccgct catgggcacc tctctttaag ccatcttaaa tgactctgag gtgtctgtta
      181 aacccgtggc gattcagtga gcgcattctg accgaccgtg aaaatggtga aaaaggcgat
      241 aattctcaag atacctctta tgcccgtgta ggcgtgaaag gtgaaactca gatcaaccct
      301 gaaatgactg gttacggtca gtttgaacta gacctggaag catcaaacag acacaaccct
      361 gaccaaactc gtttggcata tgctggtttg agttacaaag attttggttc attcgactac
      421 agtcgtaatg tcggtgtcgc gtatgatgca gaagcattta ctgatatgtt tgttgagtgg
      481 ggcggtgact catgggctgg tactgacctg ttcatgacca accgtaccaa cggtgttgca
      541 acctatcgta acacagattt ctttggcatg gttgaaggtc taaattttgc tctgcaatat
      601 cagggtaaaa atgaaggtac aggcaactac aaagcaaatg gcgatggcca tggtttatct
      661 gcaacttaca ctattgacgg tttctcattt gctggtgctt atgcaaactc tgaccgtact
      721 gattggcagt ctggtgatgg taaaggcgaa agagccgaag tttgggcgtt gtcaacaaaa
      781 tatgacgcta acaatgttta tgctgctgtg atgtacggtg aatctcacaa catgaattcc
      841 gacgacggtg atgttgtgaa caaaacacaa aacttcgaag ctgttctgca atatcagttc
      901 gactttggtc tgcgtccatc catcggttac tcctattcta aagcactgga cgtagca
//
LOCUS       DAETQL010000297          923 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.301, whole genome shotgun sequence.
ACCESSION   DAETQL010000297 DAETQL010000000
VERSION     DAETQL010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.301"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..200
                     /locus_tag="K0W65_004474"
                     /pseudo
     CDS             1..200
                     /locus_tag="K0W65_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            300..>923
                     /locus_tag="K0W65_004475"
     CDS             300..>923
                     /locus_tag="K0W65_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBI3097411.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTSTTLDYLLVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGQEENAQADAHEDCQDCEVSVATLRFTQRLLHIFTYAAGDLKYLHHAT
                     REQRKHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIK"
BASE COUNT          293 a          217 c          205 g          208 t
ORIGIN      
        1 attggctacg taattaaaac agaaaataaa gaaaccccat tcactgtagt tgatatcgac
       61 ggtctatcag gaaactttaa aactcttgtt gaaggtgcca caaaaatgtg cctagtttac
      121 atcggaaata atctgcccgc agaaaaaaag ccgaatttct ggcaaatctg attgcaatga
      181 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacag tgtgcaggcc tgagtgaaga
      241 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
      301 tgagcacaaa accactcttc ctgttacgga aagcgaaaaa atcatccggt gaacctgacg
      361 tcgtcctgtg ggcaagcgac gattttgaat cgaccagcac cactctggac tatctgctcg
      421 ttaagtcagg taaaaaactg agcaactatt ttaaagctgt tgccacaaat tttcctgtcg
      481 ttaatgacct tccccctgaa ggtgagatcg attttacctg gagtgaacgc tatcaactca
      541 gcaaagactc catgacatgg gaactaaaac cgggagcagc gccagacgac gttcaccacc
      601 aggataatgc tcaagaaacc aaagaactgg cgggaggcca ggaagaaaac gcgcaggcag
      661 acgcccacga ggattgccag gattgcgaag tctctgtagc cactttgcgg ttcacacagc
      721 gtcttctgca catttttacg tatgctgccg gggatctgaa atacctgcat catgccaccc
      781 gtgaacaacg caaacacatt actgctcttg agatggatca ggaaaacagc tatgtccaga
      841 atctgctgtt ggccatacgc ggcatggcag aaccgacaac tctggataat gccgccctgc
      901 tccgcctgac tgatgcaatt aag
//
LOCUS       DAETQL010000298          917 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.302, whole genome shotgun sequence.
ACCESSION   DAETQL010000298 DAETQL010000000
VERSION     DAETQL010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.302"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(44..917)
                     /locus_tag="K0W65_004476"
                     /pseudo
     CDS             complement(44..917)
                     /locus_tag="K0W65_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040175.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT          258 a          221 c          160 g          278 t
ORIGIN      
        1 aaaaactcta actcggtggc cagtttttgt tgaccacttc accatcaaca attttcctct
       61 gagtgtcatt tagtggacgg taagctaatg tcacctcatc aaagaacgcc cttaccctgt
      121 aactaatatc ggtcaatgaa tcgacaccat gtgacataga agtcatgaga ctaccaagag
      181 attcaccaag actcttacca cttccaccaa gagcgtttaa aagattggtt aagcttcttg
      241 taaaatcttg cagaccattt tcagaacctc tgaagatact atcaagcgtg gtattccaag
      301 agtttttcaa gttccctagt tgtgcctgtg tactttctga aatagctttc cagccacctt
      361 tagcggtgat ttgtttattc agttcctgag tgaccttctg aagagtatcg gcagatttta
      421 aagccccctt cttcatagca tcaaataatt cctgttcagt ctttccggtt gctttagcga
      481 atacctgaac cataccagga acagcttcag ctagttgccc cttaagctct tcagccataa
      541 tctgaccctt accagccatc tgtgataatg ctgtcagtgc tcgcttaatc tcttcttcac
      601 ttcgccccat cagacgagaa tagccaatca tatttctaaa aagttcttca gaaccttgaa
      661 tgcctagaga ctctctagct gaagcaccga aaccagcaaa ctgtgattgc agttcagcga
      721 tatctaaacc cgttgtttga gctaatgact ttgtgaaatc ttttacttga tttgcatctt
      781 tagaaaatac gaaatcaatt gaacgttcag cacttgcacg ttgaaggcca acttccatta
      841 ctcgctgata agcggatagt acagcctgaa agctgataag tacacctgct gttttaactg
      901 ctgctgaact taaatta
//
LOCUS       DAETQL010000299          916 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.303, whole genome shotgun sequence.
ACCESSION   DAETQL010000299 DAETQL010000000
VERSION     DAETQL010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.303"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(38..226)
                     /locus_tag="K0W65_004477"
     CDS             complement(38..226)
                     /locus_tag="K0W65_004477"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097412.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
BASE COUNT          244 a          176 c          212 g          284 t
ORIGIN      
        1 atccgtttta ttgaatcgga gaacaaactg tgagctatca gcctggtggt ctggttttgc
       61 ctgatttaag ccgccgtggg tatttgtcca agaatgcttc aagctccaat cgttttggcc
      121 tggtgaatgg agatgggtta agcaaagact ctcgttgctg gtgtactttt tcgcgctggc
      181 gctcaagcat ggataatgcg attgcctgta gttcttcgtt agtcattgtc tgcacctgga
      241 aagcaattta ccggagtacc tcacactacg gcaaaaatgt tttattgttg caacttgcgt
      301 aattattcag gtcagtttgg aacgcactgt ataaacataa ataacctgat actgttgtgt
      361 gttttcaaag aaaacacagg tatttttcac ataaaccttc cccttttcat attcgaccac
      421 ttaatcaaaa aacggtttta tacttgttgc ccccactggg gttaaatgtg agacatgtcg
      481 cactttgtgc catgtgtcct cgctgatttt aggcgcggtt cattcattct ttctgttggc
      541 ataaagtatg gtatcggttg ggcgaatgaa agggactcgg gtcatggtca gacaatacct
      601 gaggcgttaa ttcgttgaaa gagattgtga atgggggaat aactgagtct taactgctgt
      661 gcgatagccc ggacagatag ttgctcgtcg aatctgagac gtaatgattc tggcaaataa
      721 gtgcgaaagc cggtgatatc tagtgttgtt tgagagttca tcgttcgcgt ccattaatac
      781 agatggacgc gattgttgcc agacaggata attttcacaa gacatcgcag atagagtact
      841 tacgataatt ttttatttta tacaacagat gcccacgaaa agaattattt tcaatagaca
      901 cctgcattac acaaag
//
LOCUS       DAETQL010000300          880 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.304, whole genome shotgun sequence.
ACCESSION   DAETQL010000300 DAETQL010000000
VERSION     DAETQL010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.304"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>880
                     /locus_tag="K0W65_004478"
     CDS             <1..>880
                     /locus_tag="K0W65_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBI3097413.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVK"
BASE COUNT          275 a          169 c          225 g          211 t
ORIGIN      
        1 tctataacca cgaaccgtta tgcatcaggt tatgccacac tgactgaagc agtaagtgca
       61 caggatgaac ggaacagaaa aagagataag aatagtcacg atggctctac gatatcctta
      121 tcccagcccc ttggtaatat cgggaatttg aacttcaata ccacgaggta taattcttcc
      181 cggggcacag gaaacacgcg gtctaccagc ctgagttata gtacggtatg gagaggcata
      241 acattctcca ttaactgggc aaaaaacgac ttgctgacat cgcataaatg gaaagtagat
      301 cggaaattaa gcgtgggtat ttcagtgcca ttatcgctgg gtgacgaaaa tcaaatttat
      361 gcgtcttcac agatgagtcg ttcgggagag cagggtaata actatcaggt ttcattatca
      421 ggtcagaata gtggtggtgt atggtgggat gtagcgacaa atataactaa cgctcatcag
      481 tcacaaccga agagcacgat gaacattgtg caagttggca aaaatggatc atatggtcag
      541 tttagtagcc attacagctc cagcgaaaac atgaaacagc taggtgcaaa tttaagcggt
      601 gggatactta ttacaaggga tggacttaca tttggtcaaa atgtggatgg gacattagcc
      661 cttatagagg cacccggagc aacgggggtt aacgttaatg gctggccggg actgagtact
      721 gattttcgtg ggtatgcaat actgccagtg cagccatacc gaagagacga tgttattctg
      781 gatgaaaaaa cgataggtaa gaattacgac ttaccacaaa ctagtcaatt ggttgtaccg
      841 acagctggag ccgttgtgcc tgcgacattg gcagtgaaga
//
LOCUS       DAETQL010000301          847 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.305, whole genome shotgun sequence.
ACCESSION   DAETQL010000301 DAETQL010000000
VERSION     DAETQL010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..847
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.305"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..211
                     /locus_tag="K0W65_004479"
     CDS             <1..211
                     /locus_tag="K0W65_004479"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097414.1"
                     /translation="AAGNFRIPFVINTYKPEDQFFVSGIHKERNIQVCATLNGIVKII
                     KAQNLSFFVTGSFKPSGLFPPAPQK"
     gene            242..523
                     /locus_tag="K0W65_004480"
                     /pseudo
     CDS             242..523
                     /locus_tag="K0W65_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
     gene            complement(537..>847)
                     /locus_tag="K0W65_004481"
     CDS             complement(537..>847)
                     /locus_tag="K0W65_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HBI3097415.1"
                     /translation="QELWGVLLAYNLVRYQMIKMAEHLKGYCPNQLSFSESCGMVMRM
                     LMTLQGASPGRIPELMRDLASMGQLVKLPTRRGRAFPRVVKERPWKYPTAPKKSQSVA
                     "
BASE COUNT          201 a          244 c          178 g          224 t
ORIGIN      
        1 tgcagcgggc aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt
       61 ttttgtttcc ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat
      121 cgtcaaaatt atcaaagcgc agaacctcag cttcttcgta accggctcat ttaaaccgtc
      181 tggtctgttt cctccggctc cacaaaaata atgtccatca ttttgaagtg gacactatcg
      241 tatggaatac cggacctgga tcactgaagc tttacgcctt catttcgaag aacatttacc
      301 acgggttgta gccggacgtc gcctgggcgt accaaaatca actgtctgtg gtatgttcgt
      361 gcgcttccgc aacgctggcc tttcatggcc tctgcccgca ggtatgtcgg agcaggaact
      421 tgatgccctt ctttacgaaa gtgcctccac agtacctgtc gtacttactg aaagcaccgt
      481 tatgcccaaa ctcccggtgg taaaaaaacg tccccggcgg ccttaatgcc agtcagttaa
      541 gcaactgact ggctcttttt cggggctgtg gggtatttcc agggcctctc ctttaccact
      601 ctcgggaagg cccttcccct tctcgtcggt aatttcacaa gttgtcccat acttgcaaga
      661 tcgcgcatca gctccggtat acgtcccggt gaagcgccct gcaatgtcat cagcattctc
      721 atcaccattc cgcatgattc tgagaaactc agttgattcg ggcagtaacc tttcagatgt
      781 tccgccattt taatcatctg atatctcacc agattataag ccagtaagac accccacagc
      841 tcttgct
//
LOCUS       DAETQL010000302          834 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.306, whole genome shotgun sequence.
ACCESSION   DAETQL010000302 DAETQL010000000
VERSION     DAETQL010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.306"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..665
                     /locus_tag="K0W65_004482"
                     /pseudo
     CDS             1..665
                     /locus_tag="K0W65_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT          252 a          163 c          178 g          241 t
ORIGIN      
        1 ggtttatctg atgggttaat tgatggtctg aagatgcttg ctgtggctgt tactgctcaa
       61 aaggctgcaa atgtagcagg aggcttgtta aaccttgcaa acgctgttac ttcattcggt
      121 gacgctgtta ataaagctaa agctggcaag gctggttctt caggtggtgc gctaaataaa
      181 gtcggtagag ttgtccttta tgctgagttg gtaaattttg ctcttgataa tttaaaccct
      241 gaagcagcta agaatccgaa cttacatcaa tcaggtattg gcacagaaac aaacttcaac
      301 cctgaataca aaggtgattc atctaagcca atagttctat cgctgactgg tttaatggtg
      361 caactaataa aattgctaac ttccaagaac cgatgaataa cttcgtaaat aacacacaat
      421 caactccatc agctatcaca ggtgacatgg ttaatagcaa tgctcaatta gctatggctg
      481 tatcagagtt aggaagaaag gtagacgctt taacagcaga aactaagaag cctttatcag
      541 ttaatatcac tggtgaagca actgttagac cggatgcacc atctttcttt agcttcaatc
      601 aatcagctaa tgaccaattc acgcaggata tgctcttaag ttcaagctat ccagaggaag
      661 aatgatttct tatatatccg tgtgctacgt gaaatattct gaacagatat acccactgaa
      721 aaatagtgaa ccgccccggg tttcctggag agtgttttat ctgtgaactc aggctgccag
      781 atcatcgttt ccgatggaag cataataagc tttttctgct tctgccggag ggat
//
LOCUS       DAETQL010000303          831 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.307, whole genome shotgun sequence.
ACCESSION   DAETQL010000303 DAETQL010000000
VERSION     DAETQL010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..831
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.307"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..326
                     /locus_tag="K0W65_004483"
     CDS             <1..326
                     /locus_tag="K0W65_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBI3097416.1"
                     /translation="SETFSVVMTKDPGAACTEQFNILGSFFTTDILSDYSHLDMGNGL
                     LLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVIL
                     KITYN"
     gene            complement(369..569)
                     /gene="glgS"
                     /locus_tag="K0W65_004484"
     CDS             complement(369..569)
                     /gene="glgS"
                     /locus_tag="K0W65_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBI3097417.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          252 a          184 c          154 g          241 t
ORIGIN      
        1 ttagtgagac gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat
       61 tcaatatttt agggagcttt ttcactacgg atattttgag tgattatagc catctggata
      121 tgggtaatgg actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc
      181 gcttctcaca atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca
      241 gggcagaact tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg
      301 taatcctcaa aatcacctat aactagcatc taatacaaac actaaaacgg gccatcaggc
      361 ccgttatatc agtgctctaa ctccagctct cttgcctgca tcatctgttg gcaataccag
      421 gcataacgtg cgcaaaacca agttctatgt tcttcatcca tgttaccagt aacagccaga
      481 atatcgaccg ggcgaccttc tgcgtgcatt ctggcaaaac tacgcgccag gaaatcgaaa
      541 ttattcaaag aattaagact atgctccata agcacatcct ccggttatcg catacctttt
      601 aaggccatat gctttttctg atttcagtat tccccaatca gtgtagtgcc tttaaacata
      661 acgtgcgtaa atatattgtc cccgatcaca ctttttagtg aaaaataaaa aaccatatta
      721 aaatactttc tcttccagaa cttctctaac atgtgttatt attaacaaag acaaatgtaa
      781 gcagggaaac tattccaagg taaatattgc gtcactaaat ggacattgga g
//
LOCUS       DAETQL010000304          828 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.308, whole genome shotgun sequence.
ACCESSION   DAETQL010000304 DAETQL010000000
VERSION     DAETQL010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..828
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.308"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            211..684
                     /locus_tag="K0W65_004485"
     CDS             211..684
                     /locus_tag="K0W65_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBI3097418.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          257 a          172 c          183 g          216 t
ORIGIN      
        1 gagcaggtac cggcatccac gttacagacg gtcccggcaa aagtgcctga cctgacccct
       61 attagcggtt accagcgccc caccagaccc ggtgacatca ccccccggat catggacgac
      121 tgggggcagt aatcatgccg ggtctggctg gctaacaccc cattataagg ggtacgaata
      181 aagtaacggg ggggagatga accctatgca atgtcctggt taatatctca gtgtgctcat
      241 cagtgcactg ataataaaaa gacggagaca gatgccattt acgataaagt tcggagttct
      301 tatttgcttt catgtattct gaaaaaaaac aaaaatgttg ggcttattct tcatgcacca
      361 tcatttgttt ctgtgtctga aaaaatcgcc agaattgtga tggcaaatta ctcccgtaac
      421 tggagtaatt ccgagttggc cagtgcggtt ttaatgagtg aatcttcttt aaaaagaaga
      481 atgtacaaag aggtcggttc tataagtaca tttgtacata aaataaagct tacagaagct
      541 atcaggaaat tacgaaggac aaatactccc atttctgtca tatcatctga gttaggttac
      601 agttcaccat cgtatttttc taaagtcttc tttaaatatt taaagacata ccctcaaaac
      661 ataaggaaga aaaatggtcg gtaatgtcac caattaggag gacttgcaga gccgcttttt
      721 tacgatactg agatcaaaca gacataatct atgcggagat ttacagcagg gcatggttca
      781 caaatctgac agcgatgaat tatcggcact gcgggcagaa aatgctcg
//
LOCUS       DAETQL010000305          813 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.309, whole genome shotgun sequence.
ACCESSION   DAETQL010000305 DAETQL010000000
VERSION     DAETQL010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.309"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..794
                     /locus_tag="K0W65_004486"
     CDS             <1..794
                     /locus_tag="K0W65_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097419.1"
                     /translation="IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRF
                     VNSQAHIIMCGRAGYEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSIRIQMEKQMNI
                     ETGQVWRTARILKDRSTRIDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELF
                     ANDGTPTWLKEKRAKEIALDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIK
                     GMDWPTIKAGRNALWIELEGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          265 a          185 c          195 g          168 t
ORIGIN      
        1 tcatcgactc catcagtcac ttctggaccg gattatgcga cgagtatgcc agacgccgaa
       61 accgtaaacg aggactggaa ttttcagact gggcgtggct caaacaggag tggcgacgct
      121 ttaccgatcg atttgttaac agtcaggcgc acattattat gtgcgggcgt gcaggatatg
      181 agtatgactt tttcgaaggt gatgatggca aacgccagtt ggcaaaaacc ggtataaaaa
      241 tgaaagcaga aacagaaacc ggttatgaac cctccatccg gatccagatg gagaagcaaa
      301 tgaacataga aaccggtcag gtgtggcgca ccgccagaat actaaaagat cgctccaccc
      361 gtattgatgg acaggtattt tcaaacccaa catttaaaaa ctttcttcct catatcgagt
      421 ctcttaacct gggaggtgaa caccccggca tcgatacctc acgggataac agtgaattat
      481 ttgccaacga cggtacaccg acatggctaa aagaaaagcg tgcaaaagaa atcgcacttg
      541 atgaaattat cgagttgctc aacaagcacc atgggggcac cagtaacgac gccaaacgcg
      601 caaaagctga tttgctggaa aaaacattca cctcccgctc atgggaaaga atcaaaggta
      661 tggactggcc gacaatcaaa gctggtcgta atgcactatg gattgaactg gaaggcgttg
      721 aatatgcatt cccggatcca caaacacaaa acgaaacgca acaagcagga tacgatgaga
      781 atattccggt gtaaaaccaa gtgcattttg gga
//
LOCUS       DAETQL010000306          798 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.310, whole genome shotgun sequence.
ACCESSION   DAETQL010000306 DAETQL010000000
VERSION     DAETQL010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.310"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(15..683)
                     /locus_tag="K0W65_004487"
                     /pseudo
     CDS             complement(15..683)
                     /locus_tag="K0W65_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inositol phosphorylceramide synthase"
     gene            complement(673..>798)
                     /locus_tag="K0W65_004488"
     CDS             complement(673..>798)
                     /locus_tag="K0W65_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HBI3097420.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          257 a          209 c          179 g          153 t
ORIGIN      
        1 gccagtacac ctcaatatgg cagtgctatt ttgattcgac gttgatcaag tttctgataa
       61 ttccaacgac ggtcgacggg caccatccag tcaatcaaca tacctaccgc cagccctgtg
      121 atgacatcaa taaaatgatg ctgccatgtc gtcagcgtcg aaatggcgat gagtaaaaac
      181 catccgccac agactttacg ccacctcaca gccagatgct gacgaaagtg acgccaaagt
      241 agccagcaga gaataatatg cagcgaagga gactggttat aaggcagatc aaacagttca
      301 agttgcgaaa atagccatcc tgtcacccca ctcacttcag gacggataaa actaaacttc
      361 agcgggtaga gaaaaaaacc gcagcaggcc attaccgttg ccagaataag ccggtggaca
      421 agtcggcgtt gttcgaatgt cgagctacaa acgaacagcg aaaatccata taaaagatcc
      481 agactccagt aaggaacaat agtccaggga agaaaaggga tcgccgtttc ccaaccgaat
      541 acctgactgg ggatatcatg gctgttaagg tcctgaaccg cggtgaactg attaagagat
      601 ccataggtga agaagaaaaa cggggccagc aacaataacc agccagcgcc ttgtagcaat
      661 acgttccgac gttcagttat cacgacgcac cgccatcgaa accgtaaaaa tgccccacac
      721 atcaatccgt tgagtgcatt tatcaaatcc ggcattacgc acgagcgaat ccatctcccc
      781 ttgcgaacgc acgcgcat
//
LOCUS       DAETQL010000307          792 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.311, whole genome shotgun sequence.
ACCESSION   DAETQL010000307 DAETQL010000000
VERSION     DAETQL010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.311"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            10..687
                     /locus_tag="K0W65_004489"
     CDS             10..687
                     /locus_tag="K0W65_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3097421.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPSGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            687..>792
                     /locus_tag="K0W65_004490"
     CDS             687..>792
                     /locus_tag="K0W65_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBI3097422.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLK"
BASE COUNT          195 a          213 c          200 g          184 t
ORIGIN      
        1 acaaaaataa tgtccatcat ttttaatgga cactatcgta tgaaacaccg gacctggatc
       61 actgaagctt tacgtcttca ctttgaagaa catttacccc gggttgtggc cgggcgtcgc
      121 ctgggtgtac caaaatcaac agtttgtagt atgtttgtgc gctttcggag agctggcctt
      181 tcgtggcctt tgccctcagg catgtcggag caggaacttg atgcctgcct ttacggacaa
      241 ttttccacgg taccagtcgt acgtcctgaa agcaccgtta tatccgaagc ccccgtggta
      301 aaaaaacgtc cccggcggcc caacttccct tatgagttta aaatcgcctt agtggagcag
      361 tcactgcagc ccggagcctg tgtggcgcag atcgcccggg aaaacggaat caacgataac
      421 ctgctcttca actggcgcca tcaataccgg aaaggtggcc tgctgccttc cggaaaaaat
      481 atgccggcac tgcttcccgt gacgttaacg ccggagccgg ataataaaat cccggctccg
      541 gcacaggaac cagagcagat aaatacaccg tccgacagtc tgtgttgtga gctggttctg
      601 ccggccggaa ctctcaggct taaaggtaaa ctgacgccgg cgttattaca gacacttatc
      661 cgcgaaataa aagggagcag ccactgatga tatctctccc tgcaggttcg cgtatctggc
      721 tggttgccgg tatcaccgat atgcgaaatg gctttaacgg cctggcctca aaagttcaga
      781 acgtcctgaa gg
//
LOCUS       DAETQL010000308          764 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.312, whole genome shotgun sequence.
ACCESSION   DAETQL010000308 DAETQL010000000
VERSION     DAETQL010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.312"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            15..712
                     /locus_tag="K0W65_004491"
                     /pseudo
     CDS             15..712
                     /locus_tag="K0W65_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          182 a          202 c          213 g          167 t
ORIGIN      
        1 tttgaggtgc tccagtggct tctgtttcta tcagctgtcc ctcctgttca gctactgacg
       61 gggtggtgcg taacggcaaa agcaccgccg gacatcagcg ctatctctgc tctcactgcc
      121 gtaaaacatg gcaactgcag ttcacttaca ccgcttctca acccggtacg caccagaaaa
      181 tcattgatat ggccatgaat ggcgttggat gccgggcaac cgcccgcatt atgggcgttg
      241 gcctcaacac gattttccgc catttaaaaa actcaggccg cagtcggtaa cctcgcgcat
      301 acagccgggc agtgacgtca tcgtctgcgc ggaaatggac gaacagtggg gatacgtcgg
      361 ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac aggctccgga agacggttgt
      421 tgcgcacgta ttcggtgaac gcactatggc gacgctgggg cgtcttatga gcctgctgtc
      481 accctttgac gtggtgatat ggatgacgga tggctggccg ctgtatgaat cccgcctgaa
      541 gggaaagctg cacgtaatca gcaagcgata tacgcagcga attgagcggc ataacctgaa
      601 tctgaggcag cacctggcac ggctgggacg gaagtcgctg tcgttctcaa aatcggtgga
      661 gctgcatgac aaggtcatcg ggcattatct gaacataaaa cactatcagt aagttggagt
      721 catcacctac aactgggtac agcatgaacc ctcagacaga aaac
//
LOCUS       DAETQL010000309          706 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.313, whole genome shotgun sequence.
ACCESSION   DAETQL010000309 DAETQL010000000
VERSION     DAETQL010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.313"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            88..153
                     /locus_tag="K0W65_004492"
                     /pseudo
     CDS             88..153
                     /locus_tag="K0W65_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            381..629
                     /locus_tag="K0W65_004493"
     CDS             381..629
                     /locus_tag="K0W65_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBI3097423.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          226 a          150 c          160 g          170 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcacta
      421 aaatgccaaa cggtgctgtg gatgcgttaa aggaagaatt aacccgacgc atcagtaaac
      481 gttatgacga tgtagaggtg atcgtaaaag ccaccagcaa cgatggcctt tctgttacac
      541 gcaccgcaga taaggattct gcaaaaacat ttgttcagga gactctgaaa gatacctggg
      601 agtctgctga cgagtggttt gttcactaat aaacacgtaa aatcggtaac ggctggaaat
      661 cattcaatac tcgcactatc gaaagtttac cagccaaccg cagcac
//
LOCUS       DAETQL010000310          704 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.314, whole genome shotgun sequence.
ACCESSION   DAETQL010000310 DAETQL010000000
VERSION     DAETQL010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.314"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..443)
                     /locus_tag="K0W65_004494"
     CDS             complement(<1..443)
                     /locus_tag="K0W65_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBI3097424.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALAFQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPD"
BASE COUNT          214 a          172 c          131 g          187 t
ORIGIN      
        1 tcatctggat aatcggcatg agcaataaca tgcttgccgg tgcgactgtg tacttcaaga
       61 ataaagggca aatgctgaca tttttccagc gccgttttcg cttgtttctg ttgattatct
      121 gccagcgcaa taaaccagtc gccgccattc atcaaccaca aagacatctg ctggaatgcc
      181 agcgcatcca tcgccatctg ttcatgattg cctcttaccg cacgaaccca atgttgttcc
      241 agtaactgca gacaacgtaa actttgcggc ccacgatcga taacgtctcc cactgagata
      301 agtaaatctc gccacggatc aaaacgacaa tgccataatt tgcggcgcaa ctgctcaaga
      361 caaccgtgta tatcgccaga aagccagata tgtcgccatt gatgacccgc aattctctga
      421 taaacgggcg caggctgttt catcaatatt ttcctcccgc gctaaagatc acataatctt
      481 aacaagaatg ttaaaaaacg ctggactcag acagtagagt gtgtgttatg gttgactata
      541 aagtcagcga aggaaatgct tctggctttt aacagataaa aagagaccga acatgattcc
      601 tgttttcgtc aacaaacaat aaaacccttt aaaattaatg cgttgaataa atttacgttc
      661 atcttttaat ccctgacacg taccattgca tattaataca ttca
//
LOCUS       DAETQL010000311          699 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.315, whole genome shotgun sequence.
ACCESSION   DAETQL010000311 DAETQL010000000
VERSION     DAETQL010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.315"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..305)
                     /locus_tag="K0W65_004495"
     CDS             complement(<1..305)
                     /locus_tag="K0W65_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097425.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVM"
     gene            complement(311..571)
                     /locus_tag="K0W65_004496"
     CDS             complement(311..571)
                     /locus_tag="K0W65_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor protein Gam"
                     /protein_id="HBI3097426.1"
                     /translation="MAWARHYQQIVREEKETELADDLEKGLPQHLFESLCIDHLQRHG
                     ANKQAISHAFDDDVEFQERMAEHIRYMAETIAHHQVDIDLED"
BASE COUNT          177 a          173 c          155 g          194 t
ORIGIN      
        1 atcattacgg aaccgctgct ttccgcttca ttaatggctt caagcagatc aacaaacgag
       61 cgggtttttg ccgtaaataa ttcgatattt tccgcatcaa agcggggttt aacccagtca
      121 gagcctgttt ctgtgtccag aaacattacc gggcggttac cagcttcaat tccccgttgc
      181 cgcataagca ttaccagacc aatagccagt tcgcttgctg tgtaggtttt gccgtcgcca
      241 gcaaacccca taattcctgc ttttagatag gcctgtgtgt ttattgctcg ttgaaaaagc
      301 gccatagatt ttagtcctcc aaatcaatat caacctggtg gtgggcaatg gtttcagcca
      361 tgtaccggat gtgttctgcc atgcgctcct gaaactcaac atcatcatca aatgcgtggc
      421 tgattgcctg tttattggca ccgtggcgtt gcaaatggtc gatgcagagc gattcaaaca
      481 ggtgctgagg cagacctttt tccaggtcgt ccgccagttc cgtttctttt tcttcacgaa
      541 cgatctgctg gtagtgacga gcccatgcca tttcttcaat acgatcaaaa atcggataag
      601 cgctctgagg tagcctgagt ttaacggaca ctccttcctg aaatagaatg gcatcagaag
      661 gagctaataa tgagcagaaa aacccaacgt tactctaaa
//
LOCUS       DAETQL010000312          695 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.316, whole genome shotgun sequence.
ACCESSION   DAETQL010000312 DAETQL010000000
VERSION     DAETQL010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.316"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            32..493
                     /locus_tag="K0W65_004497"
     CDS             32..493
                     /locus_tag="K0W65_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBI3097427.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(534..>695)
                     /locus_tag="K0W65_004498"
     CDS             complement(534..>695)
                     /locus_tag="K0W65_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBI3097428.1"
                     /translation="ILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGL
                     AARQHVKKS"
BASE COUNT          204 a          152 c          159 g          180 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa ttgatcgccg
      601 cattcgcgga agtttttatg atccggtgcg cgcatcatcg ctgtaatttc cggcatcgaa
      661 acgcggaact gctgttcggt gaggatagcc agaat
//
LOCUS       DAETQL010000313          694 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.317, whole genome shotgun sequence.
ACCESSION   DAETQL010000313 DAETQL010000000
VERSION     DAETQL010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.317"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..525
                     /locus_tag="K0W65_004499"
                     /pseudo
     CDS             1..525
                     /locus_tag="K0W65_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            589..>694
                     /locus_tag="K0W65_004500"
     CDS             589..>694
                     /locus_tag="K0W65_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBI3097429.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDIT"
BASE COUNT          194 a          143 c          166 g          191 t
ORIGIN      
        1 tgtgctgatc gtgcggatga ttactaccat gaccgtctgg cgctgactgt tgataattca
       61 acgatcgacg acaactacga acactacaca tataacggca cttacaataa tgccgctgat
      121 actcatgttg tagacgttta caacattggt acggctatta ctctggatca ggaagttgat
      181 ctgtccatca ctaataactc tcacgttgcg ggtattacgt tgactcaggg ttatgagtgg
      241 gaagatattg acgacaacac tgtcagcact ggcgtaaaca gcagcgaagt gtttaataac
      301 accatcactg ttaaagattc taccgtaacc tctggttcat ggactgatga aggtactact
      361 ggttggttcg gtaatacagg taatgccagc gattatagtg gtaaatctaa ctttgtcacc
      421 gtagatactg acggagatgg tgttgctgat agcactattg caagctggga tgatgttgca
      481 ttagctgtag ttgcacatcc gaatgttgat aatgctatgc agacctgaat cgccacgggt
      541 ttaacagaca cctcagagtc atttaagatg gcttaaagag aggtgcccat gagcggtaag
      601 cgttatcccg aagagtttaa aactgaagca gtcaaacagg ttgttgatcg cggttattct
      661 gttgccagcg ttgcaacacg tctcgatatc acca
//
LOCUS       DAETQL010000314          670 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.318, whole genome shotgun sequence.
ACCESSION   DAETQL010000314 DAETQL010000000
VERSION     DAETQL010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.318"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..141
                     /locus_tag="K0W65_004501"
     CDS             <1..141
                     /locus_tag="K0W65_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBI3097430.1"
                     /translation="RNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFYQRL
                     RN"
     gene            256..>670
                     /locus_tag="K0W65_004502"
     CDS             256..>670
                     /locus_tag="K0W65_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HBI3097431.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPS"
BASE COUNT          170 a          179 c          174 g          147 t
ORIGIN      
        1 cgtaatgtcg ttgaacggtg ctttgggcgg ctgaaagaat atcgccgcat cgccacgcgt
       61 tacgacaaaa cggcgagaaa ttacctggcg atggtgaaac tgggctgcat ccgactcttt
      121 tatcaacgct tacgtaatta agggacacag cctaaatagc tgcgcggaat agtagatccc
      181 tttgagggaa cttagcccgg attgtgcgat ctgatcaatc gccaaatcaa aacaaatcac
      241 caaccggact gagcaatgcc gatcatagca ccaatttccc gtgacgaacg acgcctgatg
      301 cagaaagcca tccataaaac acacgataaa aattatgccc gcagactgac tgccatgctg
      361 atgctgcacc ggggcgaccg tgtcagcgac gttgccagaa cgctctgctg cgcccgttcc
      421 tctgttggac gctggattaa ctggttcacg cagtcgggtg ttgagggact gaaatcatta
      481 cctgccgggc gtgcccgtcg ctggccgttt gagcatatct gcacactgtt acgtgagctg
      541 gtaaaacatt ctcccggcga ctttggctac cagcgttcac gctggagtac agaactgctg
      601 gcaataaaaa tcaatgagat aaccggctgc cagttaaatg ccggaaccgt tcgccgctgg
      661 ttgccgtctg
//
LOCUS       DAETQL010000315          668 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.319, whole genome shotgun sequence.
ACCESSION   DAETQL010000315 DAETQL010000000
VERSION     DAETQL010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.319"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>668
                     /locus_tag="K0W65_004503"
     CDS             <1..>668
                     /locus_tag="K0W65_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HBI3097432.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKR"
BASE COUNT          133 a          230 c          177 g          128 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgg
//
LOCUS       DAETQL010000316          656 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.320, whole genome shotgun sequence.
ACCESSION   DAETQL010000316 DAETQL010000000
VERSION     DAETQL010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.320"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(151..648)
                     /locus_tag="K0W65_004504"
     CDS             complement(151..648)
                     /locus_tag="K0W65_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005075795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBI3097433.1"
                     /translation="MTTELLAQLYGTEPVRIRQNHHENKVRFVEGKHFFKVVGNDLKE
                     LRVALNYSQNPVSPKARSLILWTERGAARHGKMLETDQAWDVFEKLEDCYFSQRDPST
                     PVSCQKSYDTRVLCYQRGGVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVE
                     RLMQL"
BASE COUNT          173 a          160 c          144 g          179 t
ORIGIN      
        1 gtttaacgcc ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca
       61 agtgtccatg tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag
      121 cagtttatgt gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt
      181 atctctgcga acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga
      241 aataacaata tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata
      301 acagagaact cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg
      361 actgaaatag cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat
      421 tttgccgtgg cgtgctgcgc ctcgttctgt ccagaggatg agggagcggg ctttgggtga
      481 aactggattt tgtgagtagt ttaaagcgac ccgcaattct ttaaggtcat taccaacaac
      541 tttgaaaaag tgtttccctt caacgaagcg tactttgttc tcatgatgat tctggcgaat
      601 acgcaccggc tcagtgccgt aaagctgcgc caaaagttcg gtggtaataa caggaa
//
LOCUS       DAETQL010000317          651 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.321, whole genome shotgun sequence.
ACCESSION   DAETQL010000317 DAETQL010000000
VERSION     DAETQL010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.321"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..138)
                     /locus_tag="K0W65_004505"
                     /pseudo
     CDS             complement(1..138)
                     /locus_tag="K0W65_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(190..585)
                     /locus_tag="K0W65_004506"
     CDS             complement(190..585)
                     /locus_tag="K0W65_004506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097434.1"
                     /translation="MNVVSNTQLLEQRIADFFTLSDEHKKARVLLDTLACSCPARIFG
                     GMVRDLGLYGVDGFSSDLDIVIGRSREELFQTLAELPVKQLRFNKFGGIRFRYHDFEF
                     DIWNLNETWAFQEKLIFCEDESSLLNEVA"
BASE COUNT          184 a          150 c          141 g          176 t
ORIGIN      
        1 ggtaacccat tgtcctaaag tgccttcagg aagggataat cgggaagcgc cttcactgat
       61 cgaaagttga ttttcaagaa ccgttctgac agcttcggct ttgaactatt tagagtaacg
      121 ttgggttttt ctgctcatta ttagctcctt ctgatgccat tctatttcag gaaggagtgt
      181 ccgttaaact caggctacct catttaacag acttgattca tcctcacaaa atataagttt
      241 ttcctggaat gcccatgtct cattgaggtt ccagatatca aattcgaagt cgtgataacg
      301 aaaacggatc ccaccaaact tattaaaacg cagttgtttg acgggcagtt cagccaatgt
      361 ctggaagagt tcctcacggg agcgaccgat gacgatatca aggtctgaag agaatccatc
      421 gacgccatac aagcctaagt cacgcaccat cccgccaaaa atcctggccg gacaggaaca
      481 ggccagagta tcaagtaata ctctggcttt cttatgctca tcactaagcg taaaaaaatc
      541 ggcgatacgc tgttcaagca gttgggtgtt tgagaccaca ttcatacagt gattttagct
      601 catcgtgaag gcgcattaaa taactatccg tcatgcctga gattgcgtcg g
//
LOCUS       DAETQL010000318          649 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.322, whole genome shotgun sequence.
ACCESSION   DAETQL010000318 DAETQL010000000
VERSION     DAETQL010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.322"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(24..>649)
                     /locus_tag="K0W65_004507"
     rRNA            complement(24..>649)
                     /locus_tag="K0W65_004507"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          142 a          193 c          153 g          161 t
ORIGIN      
        1 ctacaaagaa cgcttcttta aggtaaggag gtgatccaac cgcaggttcc cctacggtta
       61 ccttgttacg acttcacccc agtcatgaat cacaaagtgg taagcgccct cccgaaggtt
      121 aagctaccta cttcttttgc aacccactcc catggtgtga cgggcggtgt gtacaaggcc
      181 cgggaacgta ttcaccgtgg cattctgatc cacgattact agcgattccg acttcatgga
      241 gtcgagttgc agactccaat ccggactacg acgcacttta tgaggtccgc ttgctctcgc
      301 gaggtcgctt ctctttgtat gcgccattgt agcacgtgtg tagccctggt cgtaagggcc
      361 atgatgactt gacgtcatcc ccaccttcct ccagtttatc actggcagtc tcctttgagt
      421 tcccggccgg accgctggca acaaaggata agggttgcgc tcgttgcggg acttaaccca
      481 acatttcaca acacgagctg acgacagcca tgcagcacct gtctcacggt tcccgaaggc
      541 acattctcat ctctgaaaac ttccgtggat gtcaagacca ggtaaggttc ttcgcgttgc
      601 atcgaattaa accacatgct ccaccgcttg tgcgggcccc cgtcaattc
//
LOCUS       DAETQL010000319          644 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.323, whole genome shotgun sequence.
ACCESSION   DAETQL010000319 DAETQL010000000
VERSION     DAETQL010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.323"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>644
                     /locus_tag="K0W65_004508"
     CDS             <1..>644
                     /locus_tag="K0W65_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HBI3097435.1"
                     /translation="LRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVTNSCGS
                     RACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVCRLAVE
                     NLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFLKDAIR
                     SRWMWNMRQRLLKAWSEGLAMPESLSHITTESQRRSLVLKAGGKYWHVYMSKKT"
BASE COUNT          152 a          163 c          200 g          129 t
ORIGIN      
        1 tctgcgcgat atcgaggttg aggctgtcac aaaaatgctg gcctgcggca cgcggatact
       61 gggcgtgaag gaatacatct gcgataaacc tgagtgcccc cacgtcagat acgtcactaa
      121 ctcatgcggc agccgtgcct gcccgtcctg cggaaaaaag gccacagacc tgtggatagc
      181 gacacagctg aatcgtcttc ctgactgcga ctgggtacac ctggtcttca ccctgccgga
      241 cacgcagtgg ccggtgttcg aaagcaaccg gtggctgctg aatgacgtgt gccgtctggc
      301 ggtggagaat ctgctgtatg ccgcccggaa acgggggcag gaacccggta tcttctgcgc
      361 catccacacg tatggccgtc gtctcaactg gcatccgcat gtacatgtgt ctgtaacctg
      421 tggaggtctg aataagcatg gtcagtggaa aaagctgagc ttcctgaaag acgcgatacg
      481 ttcgcggtgg atgtggaata tgcggcaacg gcttctgaaa gcgtggtcag aggggctggc
      541 gatgccggag tcgttgtcac atatcacgac ggaatcacag aggagaagcc tggtgctgaa
      601 agccggagga aaatactggc atgtgtacat gtcgaaaaaa acgg
//
LOCUS       DAETQL010000320          625 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.324, whole genome shotgun sequence.
ACCESSION   DAETQL010000320 DAETQL010000000
VERSION     DAETQL010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.324"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..252)
                     /locus_tag="K0W65_004509"
     CDS             complement(<1..252)
                     /locus_tag="K0W65_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBI3097436.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKILLSNENVDVQNVWRDQQETLLTYLQS"
     gene            complement(273..>625)
                     /locus_tag="K0W65_004510"
     CDS             complement(273..>625)
                     /locus_tag="K0W65_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI3097437.1"
                     /translation="TVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRH
                     RKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNRR
                     LSESVPCMPHKKAG"
BASE COUNT          141 a          146 c          142 g          196 t
ORIGIN      
        1 gctttgcagg taagtcagta atgtttcttg ctgatcgcgc cagacgttct gcacatcaac
       61 attttcatta ctgagcagta ttttaacttt gctgacctgc acgccgttgt cgatccagcg
      121 tttgatctcg cggatccggt caatatcggc atcgttgaac agccgatgac cgccgtctgt
      181 ccgttgcggt ttcagcaatc cgtaacgcct ctgccacgcg cgtaacgtga cagggattaa
      241 aggtaaacca attgattttg caagttttta ttttaccccg ctttcttatg gggcatgcat
      301 gggacacttt cagatagtct tctgttaaga agttctatct gttcgtgatt gttgtctttc
      361 atccatgctc cgtaaacatt gaataccatt tgtgcgtttg tgtggcccat ctggcttgcg
      421 ataaaactag gattagctcc tgcggcaagt gaccagcatg cataggtatg cctggattga
      481 tacgattttc tgtgtctcag acctgcgcgt tttaagatac ttgtccatga ctccctgatg
      541 gagtcaacct tatagtgcgg tccggaaaac tgccgctgtt ttattacctg aggactaaaa
      601 acaaaagtgc atttatgcac agtac
//
LOCUS       DAETQL010000321          602 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.325, whole genome shotgun sequence.
ACCESSION   DAETQL010000321 DAETQL010000000
VERSION     DAETQL010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.325"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..127
                     /locus_tag="K0W65_004511"
     CDS             <1..127
                     /locus_tag="K0W65_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HBI3097438.1"
                     /translation="VTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPWNFSSVK"
     gene            complement(161..364)
                     /locus_tag="K0W65_004512"
                     /pseudo
     CDS             complement(161..364)
                     /locus_tag="K0W65_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
     gene            464..>602
                     /locus_tag="K0W65_004513"
     CDS             464..>602
                     /locus_tag="K0W65_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBI3097439.1"
                     /translation="MRMNMSDFATSFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LK"
BASE COUNT          157 a          146 c          137 g          162 t
ORIGIN      
        1 ggtcacctgc aaacagaacg aagtggagcc ggaggactgg ttgcgcgaag tgatcgagaa
       61 gctcaatgac tggccgtcga accaagtgca tgaactgctg ccctggaact tctcgtctgt
      121 aaaataatcc ttacgctacg tacttctcgg ggcgcttacc tggagaataa cgtttatcca
      181 tattcttcca gtctggttca tcttccgacg tcatcaccgg gttgaaatta cgccgatata
      241 aatcgagttc cgtcacctgt attttccgct ccattgcccg ctgatggatc gcatcagcaa
      301 tatttgcggt caatgaatca tgacgaggat gggcccatac caaataaata cgttcagact
      361 gcataatttt acctccacgt taagttctgg agcgcacttt acgcaggcgg cataatcctg
      421 taaacgtgat gattcaggat gagttaatga aggattttca ctaatgcgga tgaatatgtc
      481 tgactttgcc acttcctttg ccgtggcccg taatcaaagc tttcgtgcag caggcgatga
      541 gttaggctta tcctcgtccg ccattagcca tagtattaaa acactggaac aacgtcttaa
      601 aa
//
LOCUS       DAETQL010000322          602 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.326, whole genome shotgun sequence.
ACCESSION   DAETQL010000322 DAETQL010000000
VERSION     DAETQL010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.326"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            18..>602
                     /locus_tag="K0W65_004514"
     CDS             18..>602
                     /locus_tag="K0W65_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HBI3097440.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITR"
BASE COUNT          130 a          162 c          174 g          136 t
ORIGIN      
        1 gtcgccccgc ccttctgatg ctcccccgtt ttgccgatat ttttcagcag ggaaaccgct
       61 ggcttaactg gctggagaaa caaccggaag gggcagtccg tccggtggta attgaatcag
      121 tgacaaaaat catggcctgc gggaccacac tgatgggcta cacgcagtgg tgttgttcat
      181 caccggactg ttgccataca aaaaaggtct gcttccggtg taaaagccgc tcctgcccgc
      241 actgcggggt gaaggctggc gcacagtgga tacagtatct gctgagcctg gtcccctact
      301 gcccgtggca gcatattgtg ttcacacttc cctgccagta ctggtccctg gtgttccaca
      361 accggtggtt actggcagag atgagccgca ttgcagcgga tgtgatactg gaaatctgcc
      421 atcaggcaga tgtggagccg gggatattca cggtgatcca cacatggggg cgtgaccagc
      481 agtggcatcc gcatatccat ttatcgacaa ctgccggtgg tgtgacgtcg ggccacacct
      541 ggaaaaatct tcatttttac gcccgtaagg tgatgagcat gtggcgctac cggataacac
      601 gg
//
LOCUS       DAETQL010000323          591 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.327, whole genome shotgun sequence.
ACCESSION   DAETQL010000323 DAETQL010000000
VERSION     DAETQL010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.327"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
BASE COUNT          217 a          105 c          121 g          148 t
ORIGIN      
        1 atcccgtact ctctggccag ttgggccacg caggccccta gctggtaaga tttttcaata
       61 agatcaagct taaactccat agagtcatag agtaagaggc acgaatcctg cgggggcgaa
      121 gagaatctga cattgataga gtccattaac gttataagag gactctatca tcgccaatca
      181 tcagacattg aaatagacga cactgagatg acgcttacaa ttcttttggt ttctgtaata
      241 agtaatctga catcccccgt gcccagaaga ggagagggat atcaagccca agggttaata
      301 attgatacta cggttaaaat tgccttcagt aaaggaaacg tgattaaatt caggggattt
      361 cagagtgaaa accatacaac atattaaaat ctgttcattt agctataata catacaagaa
      421 agtgtccagt tagataatgt tatctaaata accacagata aaaacgcaca taattggggg
      481 ggggggatga aaatacctga agcagtaaat catattaatg ttcagaataa tattgatctt
      541 gttgatggaa aaacgaatcc gaataaagca acaaaagcat tacaaaaaaa t
//
LOCUS       DAETQL010000324          566 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.328, whole genome shotgun sequence.
ACCESSION   DAETQL010000324 DAETQL010000000
VERSION     DAETQL010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.328"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..297
                     /locus_tag="K0W65_004515"
     CDS             <1..297
                     /locus_tag="K0W65_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HBI3097441.1"
                     /translation="SSAQYSGEIKGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPG
                     IDDSVDLSRYSPELDSHGQYSLPASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(405..>566)
                     /locus_tag="K0W65_004516"
     CDS             complement(405..>566)
                     /locus_tag="K0W65_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097442.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          185 a          133 c          104 g          144 t
ORIGIN      
        1 agcagcgctc agtactccgg cgaaataaag ggatacgatt acgatacata taccttctat
       61 gccaaaaaag gtcagaaagt gcatgtgagt atctctaatg aaggcgctga tacttatctc
      121 ttcgggccag gaatcgatga ttctgttgat ttatccagat attccccaga actcgatagt
      181 cacggacaat actcactccc tgcttctggc aaatatgagc tgcgagtgct gcaaactcga
      241 aacgacgccc gtaaaaacaa aactaaaaaa tataacgtcg atattcaaat taagtaaatg
      301 tcaaccgacg gggactatcc ccgtcataga cgttaacgcc ccactcctct ctgtcgctca
      361 agaggttaat gacgaatagc agagaacata atgtaaacaa ggcatcactc acgacacata
      421 aataacatct ttgtggtggt ttgattgatt tttccactcc gcttaccatc tacaccaaaa
      481 taacgatcat cacgacataa tttttcttta acatcgcaaa atacaccgtt agcgaatgta
      541 aattcgctgg gatcgtagcc ttgtaa
//
LOCUS       DAETQL010000325          557 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.329, whole genome shotgun sequence.
ACCESSION   DAETQL010000325 DAETQL010000000
VERSION     DAETQL010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.329"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>557
                     /locus_tag="K0W65_004517"
     CDS             <1..>557
                     /locus_tag="K0W65_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HBI3097443.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETVS"
BASE COUNT          164 a          137 c          148 g          108 t
ORIGIN      
        1 tgcgccaact ctgcgtatcc gtgacccgca taaagatgaa aagatggcag caatccataa
       61 agcactggac gaatgcagcg cagagcatcc ggtcttttat gaagatgaag tggatatcca
      121 tcttcatccc aaaatcggtg cggactggca actgcgcgga cagcaaaaac gggtggtcac
      181 gccgggacag aatgaaaaat attatctggc cggagcgctg cacagcggga caggtaaagt
      241 cagctatgtg ggcggcaaca gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct
      301 taaagcgaca taccgtcgtg cgaaaaccat cacgctgatc gtggacaact acattatcca
      361 caaaagccgg gaaacacaga gctggctgaa ggagaacccg aagttcaggg gcatttatca
      421 gccggtttac tcgccatggg tgaatcatgt tgaacggcta tggcaggcac ttcacgacac
      481 aataacgcgt aatcatcagt gccgctcaat gtggcaactg ttgaaaaaag ttcgccattt
      541 tatggaaacc gtcagcc
//
LOCUS       DAETQL010000326          551 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.330, whole genome shotgun sequence.
ACCESSION   DAETQL010000326 DAETQL010000000
VERSION     DAETQL010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.330"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..>551)
                     /locus_tag="K0W65_004518"
     CDS             complement(<1..>551)
                     /locus_tag="K0W65_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HBI3097444.1"
                     /translation="VEQAKDNSLQPSQTVTDSLKVADLSTIIQEQPSFVAETRAADKN
                     TDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVSQRDNKT
                     ATFDFSTCSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVREDVMDQ
                     IVQEGSLEYATDVIIHLQQIDIK"
BASE COUNT          134 a          117 c          135 g          165 t
ORIGIN      
        1 atttaatatc aatctgttga aggtgtatta ttacatcagt agcatattcc agactgcctt
       61 cctggacgat ctgatccata acatcctcac gcactttata tcttgtgcat ttcatttcaa
      121 gtgccgtgag tacagccatt actggtgatg gtagctgtgt tgttttcagg ccgtcgatct
      181 gactgatcgc ttgggcgacg gtgttttgcc attctaaaga acaggtgctg aagtcaaatg
      241 tggctgtttt attatcccgt tgcgacacgc tacaggctaa gcgacgtaat tgggtcaatt
      301 tgtctgcttg ctgaacagga actccgggaa agcggcgatg acttaacgtg gtgtgtacaa
      361 cttcgggcgg aaaaacgaca gcaatatctt tcgccagcca aggtaaaaca gcatctgtat
      421 ttttgtcagc cgcacgggtt tctgctacga aagagggttg ttcctgaata atggtggata
      481 agtccgctac cttgagagag tccgttaccg tttgggaagg ttgcaaagaa ttatctttcg
      541 cttgctccac a
//
LOCUS       DAETQL010000327          520 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.331, whole genome shotgun sequence.
ACCESSION   DAETQL010000327 DAETQL010000000
VERSION     DAETQL010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.331"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            369..>520
                     /locus_tag="K0W65_004519"
     CDS             369..>520
                     /locus_tag="K0W65_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBI3097445.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KMLEEL"
BASE COUNT          129 a          138 c          113 g          140 t
ORIGIN      
        1 ctagggtctc tctataggtc ttctctatgg tctctctata ggtctctcta gggtctcctc
       61 taggcattac ccccagaagc taactctatg gccctctatg gtcctctatg gccctgtacg
      121 tcattcttta ggatgccctg tagtttcgta tagcctgatg cctagagaga ccctagagag
      181 accctagagt ggctcctaga gagaccctcc cagtagctcc gcacagctac atcgtcatca
      241 tcatagtatt caatctcaaa gtcattctgt ttgcatcctg ttgtcctgct ggtctccctt
      301 cgcttgggga ctttgagtag gtcagtgggg gtgcgacttc agggtgcaaa caaaaaggaa
      361 atcaagcgat gtctcaacaa ccacaacaac cacaacaaga aatcagcatc attaacctcg
      421 accagcttgt gtccatgacc tcagttgaaa ttgctgagct gacaggcaag gagcaccgaa
      481 atgtccttcg tgatattcgt aagatgctcg aagaacttaa
//
LOCUS       DAETQL010000328          511 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.332, whole genome shotgun sequence.
ACCESSION   DAETQL010000328 DAETQL010000000
VERSION     DAETQL010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.332"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..511
                     /locus_tag="K0W65_004520"
                     /pseudo
     CDS             1..511
                     /locus_tag="K0W65_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          127 a          128 c          141 g          115 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt a
//
LOCUS       DAETQL010000329          497 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.333, whole genome shotgun sequence.
ACCESSION   DAETQL010000329 DAETQL010000000
VERSION     DAETQL010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.333"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            42..305
                     /locus_tag="K0W65_004521"
                     /pseudo
     CDS             42..305
                     /locus_tag="K0W65_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            351..>497
                     /locus_tag="K0W65_004522"
     CDS             351..>497
                     /locus_tag="K0W65_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="with Hns involved in transcriptional regulation of
                     hemolysin; non-specific DNA-binding protein which affects
                     the production of multiple proteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBI3097446.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLA"
BASE COUNT          144 a          110 c          115 g          128 t
ORIGIN      
        1 gggtttttct gctcattatt agctccttct gatgccattc tatttcagga aggagtgtcc
       61 gttaaactca ggctacctca tattttcagc gagatcgtta tggtcgtatt ctcgggcagg
      121 tttatgcacc agacggaatg aatattaatc agtttatggt ccgggccggg gctgcctggg
      181 tatatgagca gtacaacaca gatcccgtgc tacctgtcct tcagaacgaa gccagacaac
      241 aaaaacgtgg gctctggtca gatgctgatc cggttccccc ctggatatgg aggcaccgta
      301 aataaagcga tggcaatcct atcattcatc tgttctcagc gggaataaaa atggcaaaaa
      361 caaaacagga atggctgtat cagttacggc gatgttcatc agtcaatacg ctggaaaaaa
      421 ttattcataa aaacagagac agtttatcaa acagtgaacg tgaatcattt aattcagcag
      481 cagaccaccg gctagcg
//
LOCUS       DAETQL010000330          486 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.334, whole genome shotgun sequence.
ACCESSION   DAETQL010000330 DAETQL010000000
VERSION     DAETQL010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.334"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>486
                     /locus_tag="K0W65_004523"
     CDS             <1..>486
                     /locus_tag="K0W65_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBI3097447.1"
                     /translation="LGALWVRNGMEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFL
                     KIIALRLCELLTMYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLAD
                     RFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFT
                     E"
BASE COUNT          110 a          107 c          123 g          146 t
ORIGIN      
        1 actgggagca ttgtgggtgc gcaatggcat ggaggagtcc gcggaatttg aacaacagca
       61 acataatcaa gcggccgcga aaaaacgcat cccggttatc gaagctctgt tacgacatcc
      121 cggtgctttc ctgaagatta ttgcactacg actgtgcgag ttgctgacga tgtacattgt
      181 tactgccttt gcacttaatt attcaaccca gaatatgggg ttaccgcgcg aacttttcct
      241 taatattggt ttgctggtag gtggattaag ctgcctgaca attccctgtt ttgcctggct
      301 tgccgatcgt tttggtcggc gcagggttta tatcacaggc gcgttgatcg gaacgttgag
      361 cgcatttcct ttctttatgg cgcttgaagc acaatctatt ttctggatag ttttcttctc
      421 cataatgctg gcaaacattg cgcatgacat ggtggtgtgt gtgcaacaac cgatgtttac
      481 cgaaat
//
LOCUS       DAETQL010000331          485 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.335, whole genome shotgun sequence.
ACCESSION   DAETQL010000331 DAETQL010000000
VERSION     DAETQL010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.335"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            1..485
                     /locus_tag="K0W65_004524"
                     /pseudo
     CDS             1..485
                     /locus_tag="K0W65_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          140 a          126 c          124 g           95 t
ORIGIN      
        1 tcagggcaaa acaccaaaag cctcccccat taccccggaa caaattgaaa tccgtgagct
       61 caggaaaaag ctacaacgca ttgaaatgga gaatgaaata ttaaaaaagg ctaccgcgct
      121 cttgatgtca gactccctga acagttctcg ataatcggga aactcagagc gcattatcct
      181 gtggtcacac tctgccatgt gttcggggtt catcgcagca gctacagata ctggaaaaac
      241 cgtcctgaaa aaccagacgg cagacgggct gtattacgca gtcaggtact tgagctacat
      301 ggcatcagcc acggttcggc cggagcaaga agcatcgcca caatggcaac ccggagaggc
      361 taccagatgg gacgctggct tgctggcagg ctcatgaaag agctggggct ggtcagctgt
      421 cagcagccga ctcaccggta taaacgtggt ggtcatgaac atgttgctat ccctaactac
      481 cttga
//
LOCUS       DAETQL010000332          468 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.336, whole genome shotgun sequence.
ACCESSION   DAETQL010000332 DAETQL010000000
VERSION     DAETQL010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.336"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>468
                     /locus_tag="K0W65_004525"
     CDS             <1..>468
                     /locus_tag="K0W65_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBI3097448.1"
                     /translation="SGSTLISRDNKLVENFIDTLENWRDQKQIISVICKDGIKIDDSI
                     ITSFKASKDVGISNGLRIQLTFQEINFKAIVGQTDVSAATGRTSTTNDGGATSKKNTG
                     NTTTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASAN"
BASE COUNT          148 a           96 c          103 g          121 t
ORIGIN      
        1 aatccggtag tacccttatc tcacgagata ataagctggt ggagaacttc atcgacactc
       61 ttgaaaactg gcgtgaccag aagcagatta tttcagttat ctgtaaagat ggtattaaga
      121 ttgacgactc cattattaca agctttaaag cctctaaaga tgttggaatt tcaaacggac
      181 tgagaatcca gctaactttt caggaaatta acttcaaagc tattgtaggg caaactgatg
      241 tttcagcagc cactggacgg acttctacca cgaatgatgg cggtgctacc agtaaaaaga
      301 acactggcaa cactacgaca agtcttggaa gccctatgct gacctgtaaa gagcttttta
      361 gctattcggc aagtgagtta tcagatgaag cgttaaaagc ccgtgtaacg tgctctaaga
      421 gcgttagcgt taaaaatggt gagagcacct tcacagcctc agctaatg
//
LOCUS       DAETQL010000333          463 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.337, whole genome shotgun sequence.
ACCESSION   DAETQL010000333 DAETQL010000000
VERSION     DAETQL010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.337"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>463
                     /locus_tag="K0W65_004526"
     CDS             <1..>463
                     /locus_tag="K0W65_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBI3097449.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT          117 a          109 c          142 g           95 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcc
//
LOCUS       DAETQL010000334          460 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.338, whole genome shotgun sequence.
ACCESSION   DAETQL010000334 DAETQL010000000
VERSION     DAETQL010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.338"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..460)
                     /locus_tag="K0W65_004527"
     CDS             complement(<1..460)
                     /locus_tag="K0W65_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
                     /protein_id="HBI3097450.1"
                     /translation="MLSNRVASYGAIMAALPYMQLYIADYLADTMHLSAEEHGAYLLL
                     MFNYWQTGKPIPKNRLAKIARLTNERWVDVEPSLREFFCDNGDEWMHLRIEEDLASVR
                     EKLTKKSAAGKASVQARRSRKEADVQTKQERNLTGVQTDVGVVFEHDAGFA"
BASE COUNT          106 a          117 c           90 g          147 t
ORIGIN      
        1 gggcaaatcc agcatcatgt tcgaacacca ctccaacatc tgtttgaaca cctgttaaat
       61 ttctctcttg ttttgtttga acatctgctt cctttctgct tcttctggcc tgaacagatg
      121 cttttcctgc ggctgatttt ttggttaatt tttctctgac agatgccaga tcttcctcaa
      181 tccgaagatg catccattcg tcgccgttat cacaaaaaaa ctcccgcaag gatggttcaa
      241 catcaaccca tcgctcgtta gtcagacggg caatttttgc caacctgttt ttgggtattg
      301 gctttcctgt ttgccagtaa ttgaacatca gcaacaaata cgcgccgtgc tcctctgctg
      361 acaaatgcat ggtgtcagcc aggtaatcag ctatgtacag ttgcatgtat ggtaatgcgg
      421 ccataattgc cccgtatgat gctacccggt tgcttagaat
//
LOCUS       DAETQL010000335          443 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.339, whole genome shotgun sequence.
ACCESSION   DAETQL010000335 DAETQL010000000
VERSION     DAETQL010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.339"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            213..>443
                     /locus_tag="K0W65_004528"
     CDS             213..>443
                     /locus_tag="K0W65_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HBI3097451.1"
                     /translation="MTLPTTIYSFPAYLPRFSSTDKPAKLKFHQYARATLLSNRGRDH
                     NCDSRRTVEIHKLDLSDWQAFNKLATKCNAYDG"
BASE COUNT          141 a          105 c           61 g          136 t
ORIGIN      
        1 tatcgaacct cgcttaccaa cgcgttgtca gtcatctccc ccctctctgg tgcatcgtta
       61 tatccgtgat gagcgatttt tgatattaat atctttcacc atccgctatt ttcaccactt
      121 cagaaaacaa acttattttt attaaaatca attctttttc tttgaacatt caactgcgat
      181 atttgtaaaa acattaaaca ggcaacaata aaatgacatt accaacaact atttattcat
      241 tccccgcata tttaccccgt ttttcgtcca ccgacaaacc agcaaaatta aaatttcatc
      301 aatacgcacg cgctacatta ctgtcaaatc gtggaagaga tcataattgc gatagcagac
      361 gtactgttga gatccacaaa ctagatctct ctgactggca agcattcaat aagttagcaa
      421 cgaagtgtaa tgcttacgat gga
//
LOCUS       DAETQL010000336          434 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.340, whole genome shotgun sequence.
ACCESSION   DAETQL010000336 DAETQL010000000
VERSION     DAETQL010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.340"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            35..434
                     /locus_tag="K0W65_004529"
                     /pseudo
     CDS             35..434
                     /locus_tag="K0W65_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAETQL010000337          428 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.341, whole genome shotgun sequence.
ACCESSION   DAETQL010000337 DAETQL010000000
VERSION     DAETQL010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.341"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>428
                     /gene="istA"
                     /locus_tag="K0W65_004530"
     CDS             <1..>428
                     /gene="istA"
                     /locus_tag="K0W65_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HBI3097452.1"
                     /translation="SRNTVKRYLQAKSEPPKYTPRPAVASLLDEYRDYIRQRIADAHP
                     YKIPATVIAREIRDQGYRGGMTILRAFIRSLSVPQEQEPAVRFETEPGRQMQVDWGTM
                     RNGRSPLHVFVAVPGYSRMLYIEFTDNMRYDTLETCHRNA"
BASE COUNT          100 a          118 c          105 g          105 t
ORIGIN      
        1 tctcccgcaa taccgttaaa cgttatttgc aggcaaaatc tgagccgcca aaatatacgc
       61 cgcgacctgc tgttgcttca ctcctggatg aataccggga ttatattcgt caacgcatcg
      121 ccgatgctca tccttacaaa atcccggcaa cggtaatcgc tcgcgagatc agagaccagg
      181 gatatcgtgg cggaatgacc attctcaggg cattcattcg ttctctctcg gttcctcagg
      241 agcaggagcc tgccgttcgg ttcgaaactg aacccggacg acagatgcag gttgactggg
      301 gcactatgcg taatggtcgc tcaccgcttc acgtgttcgt tgctgttccc ggatacagcc
      361 gaatgttgta catcgaattc actgacaata tgcgttatga cacgctggag acctgccatc
      421 gtaatgcg
//
LOCUS       DAETQL010000338          417 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.342, whole genome shotgun sequence.
ACCESSION   DAETQL010000338 DAETQL010000000
VERSION     DAETQL010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.342"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..128
                     /locus_tag="K0W65_004531"
     CDS             <1..128
                     /locus_tag="K0W65_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="holin"
                     /protein_id="HBI3097453.1"
                     /translation="VSPSQWAAIGVLGSLLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            128..>417
                     /locus_tag="K0W65_004532"
     CDS             128..>417
                     /locus_tag="K0W65_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HBI3097454.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLVTVARQINPYIKVDIPET"
BASE COUNT          118 a           86 c          109 g          104 t
ORIGIN      
        1 aggtcagtcc atcacagtgg gctgcgattg gtgtgctggg aagtctgctt ctggggcttc
       61 tgacttatct gacgaatctg tatttcaaaa taagagaaga taagcgtaag gctgcgagag
      121 gtgaataatg tcgccatcat tacgcaaggc tgttgcagtt gctattggtg gcggggctgt
      181 tgctatagca tctgtgttaa tcactggccc aggtggtaac gatggtctgg aaggtgtcag
      241 ctacatacca tacaaagata tcattggtgt atggactgta tgtcacggac acaccggaaa
      301 agacatcatg cctggtaaaa cgtataccga agcagaatgc aaagctctcc tgaataaaga
      361 ccttgtcact gtcgccagac aaattaaccc gtacatcaaa gtcgatatac cggaaac
//
LOCUS       DAETQL010000339          416 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.343, whole genome shotgun sequence.
ACCESSION   DAETQL010000339 DAETQL010000000
VERSION     DAETQL010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.343"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..373
                     /gene="istB"
                     /locus_tag="K0W65_004533"
     CDS             <1..373
                     /gene="istB"
                     /locus_tag="K0W65_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HBI3097455.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT          121 a           99 c          105 g           91 t
ORIGIN      
        1 aacagcagca gatctgttac ttcagttatc tacggcacaa cgtcagggcc gttataaaac
       61 gacgcttcag cgtggagtaa tggccccccg cctgctcatc attgatgaaa taggctatct
      121 gccgttcagt caggaagaag caaaactgtt cttccaggtc atcgctaaac gttacgaaaa
      181 gagcgcaatg atcctgacat ccaatctacc gttcgggcag tgggatcaaa cgttcgccgg
      241 tgatgcagca ctgacctcag cgatgctggg ccgtatctta caccactcac atgtcgttca
      301 aatcaaagga gaaagctatc gactcagaca gaaacgaaag gccggggtta tagcagaagc
      361 taatcctgag taaaacggtg gatcaatatt gggccgttgg tggagatata agtgga
//
LOCUS       DAETQL010000340          390 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.344, whole genome shotgun sequence.
ACCESSION   DAETQL010000340 DAETQL010000000
VERSION     DAETQL010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.344"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(42..>390)
                     /locus_tag="K0W65_004534"
     CDS             complement(42..>390)
                     /locus_tag="K0W65_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBI3097456.1"
                     /translation="QIKPFLEKAMTMYNDVLFPGEDINKPISIAAANRFVNRIRGGMD
                     LGYWRTHDFRRTLVTRLSEMNVEPHVTERMLGHELGGIMSVYNKHDWIEAQRKAYELH
                     ADKLFWHIRSISD"
BASE COUNT           92 a           91 c           87 g          120 t
ORIGIN      
        1 ccttcgtgaa gctgttgaag ggtggattct taacggtggc gttaatcaga aatgctcctg
       61 atgtgccaga acaatttatc agcgtgaagc tcatacgctt tgcgctgagc ctctatccag
      121 tcgtgtttat tgtatacgga cattatcccg ccaagttcat gaccgagcat tcgctcagta
      181 acatgaggct cgacattcat ctcggacagc cgtgtaacaa gcgttcttct gaaatcatgt
      241 gttcgccagt aacctagatc catccctccc cttattctat ttacgaatcg gttggctgca
      301 gcgatgctga tcggtttgtt tatgtcttct ccagggaaaa gaacatcatt gtacattgtc
      361 atggcctttt cgagaaaagg cttaatttgt
//
LOCUS       DAETQL010000341          372 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.345, whole genome shotgun sequence.
ACCESSION   DAETQL010000341 DAETQL010000000
VERSION     DAETQL010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.345"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(<1..311)
                     /locus_tag="K0W65_004535"
     CDS             complement(<1..311)
                     /locus_tag="K0W65_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3097457.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAETQL010000342          368 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.346, whole genome shotgun sequence.
ACCESSION   DAETQL010000342 DAETQL010000000
VERSION     DAETQL010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.346"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(1..236)
                     /locus_tag="K0W65_004536"
                     /pseudo
     CDS             complement(1..236)
                     /locus_tag="K0W65_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           86 a          117 c           84 g           81 t
ORIGIN      
        1 tggcgtacac cacatcttcc agtaatcgcc gcttcaccgg actcaggacc ccgtatcacc
       61 atccggaacc ccttatccgc cacatgccag gagaaccttt ccataaactc gtcgccactc
      121 atcacaaggt tttcttctcg ttttgtccgg tggctgttgt aacgcaggcc aatttcatcc
      181 tgaccggcat aatgttccag gcggctcatc tgcaccggct gcttctccag ccagttaagc
      241 catcggtttc cctgctgaaa aatatcggca aaacggggaa gcatcagaaa ggcggaataa
      301 cgccatccgg ccagtgaacc gtaccgcatt cagggcaata catgccgtcg gcgttgatac
      361 cggcagga
//
LOCUS       DAETQL010000343          359 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.347, whole genome shotgun sequence.
ACCESSION   DAETQL010000343 DAETQL010000000
VERSION     DAETQL010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.347"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
BASE COUNT           76 a          102 c           90 g           91 t
ORIGIN      
        1 agcctgcgct gatgcagcat gtccctgaag gcgtgattca ggcaccagaa tcacccgacg
       61 ccccggaggc gctgccgggg tgtcgttcca tttctgatga tgacctgtgc gcctggtgta
      121 cccgtctgct ttaccgtccc ggagaaatca gtctgtgcag actgagtata caggcaatgg
      181 catctggcca tccgtctgtg accggaacgg ttacgcgtac ggctgtccgg aatttcagcc
      241 gaatatcatt catccctgat attaccgggt catcacggtc agtgatgaac gtgatgttgc
      301 cagctcccga cctttactcc ctctcattta ttcacattac aaggatttca tacatgaaa
//
LOCUS       DAETQL010000344          357 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.348, whole genome shotgun sequence.
ACCESSION   DAETQL010000344 DAETQL010000000
VERSION     DAETQL010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..357
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.348"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            39..>357
                     /locus_tag="K0W65_004537"
     CDS             39..>357
                     /locus_tag="K0W65_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HBI3097458.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          112 a           86 c           91 g           68 t
ORIGIN      
        1 gcctggcatc cggatataac ccgtgataaa acaagggaat gaacaatgaa ctccccgatg
       61 atattgagct gcttaaagcc atgttgcgta agcaacagag tcggcttcga cagtatgcct
      121 gtcaggtcgc gggctatgag caggaaattg aacggctgaa agcgcaactc gacaggttgc
      181 gtcgtatgtt gttcggccag agttcagaga aaaagcgtca taagcttgaa aatcagatcc
      241 gacaggcaga aaaacgactg tcggaactgg aaaaccggct gaacacagcc agaaatcttc
      301 tggaagatgc atcgtcagtc acagattcac ctgacaccag tcccccgtca gaaaacc
//
LOCUS       DAETQL010000345          354 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.349, whole genome shotgun sequence.
ACCESSION   DAETQL010000345 DAETQL010000000
VERSION     DAETQL010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.349"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            118..>354
                     /locus_tag="K0W65_004538"
     CDS             118..>354
                     /locus_tag="K0W65_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBI3097459.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT          100 a           57 c           93 g          104 t
ORIGIN      
        1 taagtcgatt ttttatattt tgtgattagt ttcacaaaat aaggtgttat tcaatgtgtg
       61 ctgcactatt caggggggct gaaatacagg tggcatttat ctgatatgga gaataacatg
      121 aggaaatata ttccactggc attatttatc ttttcatggc cggtattgtc tgcagatatt
      181 catggacggg ttgttcgtgt actggacggt gataccatcg aggtcatgga ctcactgaag
      241 gcggtgcgga tcaggctggt aaatattgat gcaccggaaa agaaacagga ttatggccgg
      301 tggtcaacag acatgatgaa atcactggtc gcaggaaaaa cggttaccgt caca
//
LOCUS       DAETQL010000346          353 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.350, whole genome shotgun sequence.
ACCESSION   DAETQL010000346 DAETQL010000000
VERSION     DAETQL010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.350"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..264
                     /locus_tag="K0W65_004539"
     CDS             <1..264
                     /locus_tag="K0W65_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBI3097460.1"
                     /translation="FYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISD
                     EDKEKLKACRIYAKSLQAMDFSTITDKSSYNAIEWPASPEGSS"
     gene            288..>353
                     /locus_tag="K0W65_004540"
     CDS             288..>353
                     /locus_tag="K0W65_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBI3097461.1"
                     /translation="MSVVISGALTDGAGIPMSGYHI"
BASE COUNT           96 a           80 c           90 g           87 t
ORIGIN      
        1 ttttaccgca atgatgaatt tgtctttgac tacagactca aagcggaaga tgagcgtgat
       61 gcccttctgg ctcaggtcag tgcccggaca ggggaatggg aagaagacct gctgctgggg
      121 ttaatcagcg acgaagataa agaaaagctg aaagcctgtc gtatttacgc gaaatcgctg
      181 caggcgatgg atttcagcac catcactgat aaatcctcat acaacgccat tgaatggccc
      241 gcctctccgg aaggttcttc ctgatttaat ttatcgcgag aaaaacaatg tctgtagtga
      301 tatcaggtgc gctgactgat ggcgcaggta tccccatgtc cggataccat att
//
LOCUS       DAETQL010000347          344 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.351, whole genome shotgun sequence.
ACCESSION   DAETQL010000347 DAETQL010000000
VERSION     DAETQL010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.351"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="K0W65_004541"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="K0W65_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBI3097462.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAETQL010000348          341 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.denovo.352, whole genome shotgun sequence.
ACCESSION   DAETQL010000348 DAETQL010000000
VERSION     DAETQL010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.denovo.352"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            4..171
                     /gene="xisR"
                     /locus_tag="K0W65_004542"
     CDS             4..171
                     /gene="xisR"
                     /locus_tag="K0W65_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014884030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HBI3097463.1"
                     /translation="MLRTGLGARQIEAYRQNCWVEGFHFKRVSPLGKPDSKRGIIWYN
                     YPKINQFIKDS"
     gene            173..>341
                     /locus_tag="K0W65_004543"
     CDS             173..>341
                     /locus_tag="K0W65_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HBI3097464.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHQISS"
BASE COUNT          108 a           64 c           83 g           86 t
ORIGIN      
        1 ctgatgcttc gcactggtct gggtgccaga caaattgaag cataccgcca gaactgttgg
       61 gtggaaggct tccacttcaa acgagtatct cctttaggga agccagacag taagcgaggg
      121 attatctggt acaactatcc gaagataaat cagtttatca aagactcatg atatgtctaa
      181 attaccaaca ggtgtcgaga ttcgaggtaa atacattcgc atctggttca tgtttcgagg
      241 aaaacgatgt cgggaaacat tgaaaggctg ggagattact aacagtaaca ttaaaaaggc
      301 cgggaattta agagcgttga tagttcatca aatcagttcc g
//
LOCUS       DAETQL010000349         9713 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.guided.130, whole genome shotgun sequence.
ACCESSION   DAETQL010000349 DAETQL010000000
VERSION     DAETQL010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.guided.130"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            complement(17..205)
                     /locus_tag="K0W65_004544"
     CDS             complement(17..205)
                     /locus_tag="K0W65_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBI3097465.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(424..1401)
                     /gene="epmA"
                     /locus_tag="K0W65_004545"
     CDS             complement(424..1401)
                     /gene="epmA"
                     /locus_tag="K0W65_004545"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBI3097466.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1726..3534
                     /gene="frdA"
                     /locus_tag="K0W65_004546"
     CDS             1726..3534
                     /gene="frdA"
                     /locus_tag="K0W65_004546"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBI3097467.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3527..4261
                     /gene="frdB"
                     /locus_tag="K0W65_004547"
     CDS             3527..4261
                     /gene="frdB"
                     /locus_tag="K0W65_004547"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBI3097468.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4272..4667
                     /gene="frdC"
                     /locus_tag="K0W65_004548"
     CDS             4272..4667
                     /gene="frdC"
                     /locus_tag="K0W65_004548"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBI3097469.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4678..5037
                     /gene="frdD"
                     /locus_tag="K0W65_004549"
     CDS             4678..5037
                     /gene="frdD"
                     /locus_tag="K0W65_004549"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBI3097470.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5100..6233
                     /locus_tag="K0W65_004550"
     CDS             5100..6233
                     /locus_tag="K0W65_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBI3097471.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6322..6855
                     /gene="blc"
                     /locus_tag="K0W65_004551"
     CDS             6322..6855
                     /gene="blc"
                     /locus_tag="K0W65_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBI3097472.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6852..7169)
                     /gene="sugE"
                     /locus_tag="K0W65_004552"
     CDS             complement(6852..7169)
                     /gene="sugE"
                     /locus_tag="K0W65_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBI3097473.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7345..7491)
                     /gene="ecnB"
                     /locus_tag="K0W65_004553"
     CDS             complement(7345..7491)
                     /gene="ecnB"
                     /locus_tag="K0W65_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBI3097474.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7602..7727)
                     /gene="ecnA"
                     /locus_tag="K0W65_004554"
     CDS             complement(7602..7727)
                     /gene="ecnA"
                     /locus_tag="K0W65_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBI3097475.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7779..8345)
                     /gene="efp"
                     /locus_tag="K0W65_004555"
     CDS             complement(7779..8345)
                     /gene="efp"
                     /locus_tag="K0W65_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBI3097476.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8387..9415
                     /gene="epmB"
                     /locus_tag="K0W65_004556"
     CDS             8387..9415
                     /gene="epmB"
                     /locus_tag="K0W65_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBI3097477.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2472 a         2485 c         2471 g         2285 t
ORIGIN      
        1 tgaacataaa acactatcag taagttggag tcattaccat taaggcgaat ggaataaaga
       61 ataacaatgc agaaaatata taaaagggaa tcgcactata acccattaag taataagccg
      121 atgggctatt ggcaaatcca aatacggaag taaagatcat caatatgagt cctatcagac
      181 tcatcttttt tatcgtgtga ggcatgaaac catccttcat ttcagcgcgt gtataacacg
      241 tgcccactga actgcactgc gcaacattga gcaggcagcg taaaaaccaa tacagaattg
      301 atacttggat gatatcaaat atccagtctt aaattgtggc ctttaacgga aaaatgaaaa
      361 catatgctgc agaatggcgc ttatcacgcc attcttcgct gttaattcag taatttttca
      421 gaattatgcc cggtcaacgc taaaggcgat gacttcagcc agtgtctccg cgcccagcgc
      481 caacatcacc agacgatcaa cacctaatgc cacgccggaa cagtcaggca taccgacttt
      541 caaggcttca atcagattct ggtcaatggg gtgctgcggc aaaccgcgcg ccgcgcgctt
      601 acggttatct tgttcaaagc gttgttgctg ctcacgggca tccgttaatt catggaaacc
      661 attcgccagc tcaatacctt tataataaac ctcaaagcgt tcagcgaccc gatgatcttc
      721 ggtactgatt tgcgccagtg atgcctggct ggctggaaag tggtacacaa aggtcggttt
      781 ttctttacca atatttggct ctacgccaaa ggtaaacagc aattgtagca acgtgtcgcg
      841 gtcttcttcg gtatcagcaa cattgctcaa atccagtttc gctgcgactt cccgcagttg
      901 cgttttgtcg gcagagagcg ggtcaatttc cagataacgc aagaaagctt gttgataaga
      961 aaggctttct gctgccgggc agtccagcac ctgttgtaag agatcgtcca cctcgttcat
     1021 caaccggtac atatcatagt gcggtcgata ccactccagc atagtgaact cagggttgtg
     1081 ataacgcccc atctcttcat tacggaagct gcggcacagc tggaataccg gcccacaacc
     1141 agcaaccagc aggcgtttca tatggtattc cgggctggtc attaaccaga gattcatccc
     1201 ctgcgaatgc ccggggccaa cgaaacgtgt ctcaaacggg accaaatgaa tatcggttac
     1261 cgtcgcctgg ctcatacaag gcgtctccac ctccagcact ccacgatcgg caaagaaacg
     1321 acggatctcc gccataatcg ccgcgcgttt taataagtta ggaatggatg cgctcggctg
     1381 ccaggatgcc gtttcgctca tagttaaatc tccagttttt gacaagggca cgaagtctac
     1441 tcgcaacgcg acggcgagac aaattttacg caggaatcaa acagcggtgg gcagtgacta
     1501 aaaaaagcac gatctgatgg tttagtaatt aaattaatca tcttcagtga taatttagcc
     1561 ctcttgcgca ctaaaaaaat cgatctcgtc aaatttcaga cttatccatc agactatact
     1621 gttgtaccta taaaggagca gtggaatagc gttcgcagac cgtaactttc aggcacttac
     1681 cctgaagtac ggggctgtgg gataaaaaca atctggagga atgtcgtgca aacctttcaa
     1741 gccgatcttg ccattgtagg cgccggtggc gcgggattac gtgctgcaat tgctgccgcg
     1801 caggcaaatc caaatgcaaa aatcgcacta atctcaaaag tatacccgat gcgtagccat
     1861 accgttgctg cagaaggggg ctccgccgct gtcgcgcagg atcatgacag cttcgaatat
     1921 cactttcacg atacagtagc gggtggcgac tggttgtgtg agcaggatgt cgtggattat
     1981 ttcgtccacc actgcccaac cgaaatgacc caactggaac tgtggggatg cccatggagc
     2041 cgtcgcccgg atggtagcgt caacgtacgt cgcttcggcg gcatgaaaat cgagcgcacc
     2101 tggttcgccg ccgataagac cggcttccat atgctgcaca cgctgttcca gacctctctg
     2161 caattcccgc agatccagcg ttttgacgaa catttcgtgc tggatattct ggttgatgat
     2221 ggtcatgttc gcggcctggt agcaataaac atgatggaag gcacgttggt gcagatccgt
     2281 gctaacgcgg tcgttatggc taccggcggt gcgggtcgcg tttatcgtta caacaccaac
     2341 ggcggcatcg ttaccggtga cggtatgggt atggcgctaa gccacggcgt tccgctgcgt
     2401 gacatggaat tcgttcagta tcacccaacc ggtctgccag gttccggtat cctgatgacc
     2461 gaaggctgcc gtggtgaagg cggtattctg gtcaacaaaa atggctaccg ttatctgcaa
     2521 gattacggca tgggcccgga aactccgctg ggcgagccga aaaacaaata tatggaactg
     2581 ggtccacgcg acaaagtttc tcaggccttc tggcacgaat ggcgtaaagg caacaccatc
     2641 tccacgccgc gtggtgatgt ggtttatctc gacctgcgtc acctcggcga gaaaaaactg
     2701 catgaacgtc tgccgttcat ctgcgaactg gcgaaagcgt acgttggcgt cgatccggtt
     2761 aaagaaccga ttccggtacg tccgactgca cactacacca tgggcggtat cgaaaccgat
     2821 cagaactgtg aaacccgcat taaaggtctg ttcgccgtgg gtgaatgttc ctctgttggt
     2881 ctgcacggtg caaaccgtct gggctccaac tccctggcgg aactggtggt cttcggccgt
     2941 ctggccggtg aacaagcgac agagcgtgca gcaactgccg gtaatggcaa cgaagcggca
     3001 attgaagcgc aggcagctgg cgttgaacaa cgtctgaaag atctagttaa ccaggatggc
     3061 ggcgaaaact gggcgaagat ccgcgacgaa atgggcctgg caatggaaga aggttgcggt
     3121 atctaccgta cgccggaact gatgcagaaa accatcgaca agctggcaga gctgcaggaa
     3181 cgcttcaagc gcgtgcgcat caccgacacc tccagcgtgt tcaacaccga cctgctctac
     3241 accattgaac tgagccacgg tctgaacgtt gctgaatgta tggcgcactc cgcaatggca
     3301 cgtaaagagt cccgcggcgc acaccagcgt ctggacgaag gttgcaccga gcgtgacgac
     3361 gtcaactttc tcaaacacac cctcgccttc cgcgatgctg atggcacgac tcgcctggag
     3421 tacagcgacg tgaagattac tacgctgccg ccagctaaac gcgtttacgg tggcgaagcg
     3481 gatgcagccg ataaggcgga agcagccaat aataaggaga aggcgaatgg ctgagatgaa
     3541 aaacctgaaa attgaggtgg tgcgctataa cccggaagtc gataccgcac cgcatagcgc
     3601 attctatgaa gtgccttatg acgcaactac ctcattactg gatgcgctgg gctacatcaa
     3661 agacaacctg gcaccggacc tgagttaccg ctggtcctgc cgtatggcga tttgtggttc
     3721 ctgcggcatg atggttaaca acgtgccaaa actggcatgt aaaaccttcc tgcgtgatta
     3781 caccgacggt atgaaggttg aagcgttagc taacttcccg attgaacgcg atctggtggt
     3841 cgatatgacc cacttcatcg aaagtctgga agcgatcaaa ccgtacatca tcggcaactc
     3901 ccgcaccgcg gatcagggta ctaacatcca gaccccggcg cagatggcaa agtatcacca
     3961 gttctccggt tgcatcaact gtggtctgtg ctacgccgcg tgcccgcagt ttggcctgaa
     4021 cccagagttc atcggtccgg ctgccattac gctggcgcat cgttataacg aagatagccg
     4081 cgaccacggt aagaaggagc gtatggcgca gttgaacagc cagaacggtg tatggagctg
     4141 tactttcgtg ggctattgct ccgaagtctg cccgaaacac gtcgatccgg ctgcggccat
     4201 tcagcagggc aaagtagaaa gttcgaaaga ctttcttatc gcgaccctga aaccacgcta
     4261 aggagtgcaa catgacgact aaacgtaaac cgtatgtacg gccaatgacg tccacctggt
     4321 ggaaaaaatt gccgttttat cgcttttaca tgctgcgcga aggcacggcg gttccggctg
     4381 tgtggttcag cattgaactg attttcgcgc tgtttgccct gaaaaatggc ccggaagcct
     4441 gggcgggatt cgtcgacttt ttacaaaacc cggttatcgt gatcattaac ctgatcactc
     4501 tgacggcagc tctgctgcac accaaaacct ggtttgaact ggcaccgaaa gcggccaata
     4561 tcattgtaaa agacgaaaaa atgggaccag agccaattat caaaagtctc tgggcggtaa
     4621 ctgtggttgc caccatcgta atcctgtttg ttgccctgta ctggtaagga gcctgagatg
     4681 attaatccaa atccaaagcg ttctgacgaa ccggtattct ggggcctctt cggggccggt
     4741 ggtatgtgga gcgcaattat tgcgccggtg atgatcctgc tggtgggtat tctgctgcca
     4801 ctggggctgt ttccgggtga tgcgctgagc tacgagcgcg ttctggcgtt cgcgcagagc
     4861 ttcattggtc gcgtattcct gttcctgatg atcgttctgc cgctgtggtg tggtttacac
     4921 cgtatgcacc acgcgatgca cgatctgaaa atccacgtgc ctgcgggcaa atgggttttc
     4981 tacggtctgg ctgctatcct gacagttgtc acgctgattg gtgtcgttac aatctaacgc
     5041 atcgccaatg taaatccggc ccgcctatgg cgggccgttt tgtatggaaa ccagacctta
     5101 tgttcaaaac gacgctctgc gccttattaa ttaccgcctc ttactccaca tttgctgccc
     5161 ctcaacaaat caacgatatt gtgcatcgca caattacccc gcttatagag caacaaaaga
     5221 tccccggtat ggcggtggcg gtaatttatc agggtaaacc ttattacttt acctggggct
     5281 atgcggacat cgccaaaaag cagcccgtca cacagcaaac gttgtttgag ttaggttcgg
     5341 tcagcaaaac atttacgggc gtgcttggtg gcgacgctat tgctcgaggg gaaatcaagt
     5401 taagcgatcc cgcaacaaaa tactggcctg aacttaccgc taaacagtgg aatgggatca
     5461 cactattaca tcttgcgacc tacaccgctg gcggcctgcc attgcaggtg ccggatgaag
     5521 tgaaatcctc aagcgacttg ctgcgcttct atcaaaactg gcagcctgca tgggctccag
     5581 gaacacaacg tctgtatgcc aactccagta tcggtttgtt cggcgcactg gctgtgaagc
     5641 cgtctggttt gagttttgag caggcgatgc aaactcgtgt cttccagcca ctcaaactca
     5701 accatacgtg gattaatgta cctcccccag aagaaaagaa ttacgcctgg ggatatcgcg
     5761 aaggtaaggc agtgcatgtt tcgccagggg cgttagatgc tgaagcttat ggtgtgaagt
     5821 cgaccattga agatatggcc cgctgggtgc aaagcaattt accccccctt gatatcaatg
     5881 agaaaacgct tcaacaaggg atacaactgg cacaatctcg ctactggcaa accggcgata
     5941 tgtatcaggg cctgggctgg gaaatgctgg actggccggt caatcctgac agcatcatta
     6001 acggcagtga caataaaatt gcactggcag cacgcccggt aaaagcgatt aagcccccaa
     6061 ctcctgcagt acgcgcatca tgggtacata aaacgggggc gaccggcgga tttggtagct
     6121 atgtcgcgtt tattccagaa aaagagctgg gtatcgtgat gctggctaac aaaaactatc
     6181 ccaatccagc gagagtcgcc gccgcctgtc agattctcaa cgctctacag taaaatttca
     6241 tcgggtccga attttcggac cttttctgcg cttttccttg ctgtcatcta cacttagaaa
     6301 aaaaccagta aggaaacatt tatgcgcctg ctccctctcg ttgccgcagc gacagctgca
     6361 tttctggtcg ttgcctgtag ttttcctacg ccgccgcgtg gcgtgaccgt agtgaataat
     6421 ttcgacgcca aacgttatct cggtacctgg tatgagattg cccgttttga tcaccgtttt
     6481 gaacgtggac tggaaaaagt caccgcaaca tacagcctgc gtgatgacgg cggcctgaat
     6541 gtcattaata aaggctataa ccctgacaga ggaatgtggc agcagagtga agggaaagcg
     6601 tactttaccg gcgcaccaac tcgcgctgcg ctgaaagtgt cattctttgg tcctttctat
     6661 ggcggttata acgttattgc actcgatcgg gaataccgcc atgcgctggt ttgcggcccg
     6721 gaccgcgact acctgtggat actctcccgc acgccaacca tttctgacga agtgaaacag
     6781 gagatgctgg cagtcgcgac ccgggaaggg tttgatgtca gtaagtttat ttgggtacag
     6841 cagcccggta gttagtgagt gctgagtttc agaccaataa tccccaatac gattaacgcc
     6901 agactcgcca gacgcatcgg gttagcggac tcaccgagca gcacaatgcc ggtgatggcc
     6961 gcgccgactg cgccaatacc cgtccacacg gcataagccg tccctaccgg taacgatttc
     7021 atcgcccagg caagtagcgc catactgact atcatcgccg tcacggtaat aacactcggc
     7081 gtcaaacggc taaagccgtg ggtatatttc aggccaacgg cccataccac ttccagcaga
     7141 ccagcaataa ctaagataat ccaggacata tcaggctccg gaaatttggg gccgtccccg
     7201 gtgaaaagag gcgtttgcag gtcgtcctgc aaagctgatg gttgaacgga tattttgcca
     7261 ggtggcaggg gaatttcaac tattaccttt atgacgatcg cagaaaaaac aaacggcacg
     7321 acactgctgt cgtaccgtta ttgcttattg ctgcgctttc gttgctgcgc cagaaatcgc
     7381 gttaccgcca tcagaaatgt cttcaccaac gccacgcgtg gtgttgcagg cagttaatac
     7441 tgttgaaagc accagaacag aaaagatcgc tgcaattgtc ttcttcacca taacgtcttc
     7501 cttttaggta atgttattta tgtttgccta tagcaaatta ataatagaca acatcgccaa
     7561 aaatgatgga aatctgatga tttttcggaa gagaagaaaa attagctggc agcgcgagag
     7621 atggagttac cgagatgttt gatgtcttcg ccgaaaccgc aagcggtgtt acagcccgtg
     7681 agcagcgtgc tggcaagcaa aacaagaacg ataaggcgtt tcatcatctc tgacctgaat
     7741 aagtgatggt gcagcctgca ggccgcacca caaccgcatt acttcacgcg agagacgtat
     7801 tcaccagagc gggtatccac tttgatgact tcgccgattt gtacgaacag cggaacttta
     7861 accacagcgc cagtagacag ggtagccggt ttgccgccag tacctgcggt atcacctttc
     7921 aggcccggat cggtatcaac gatttccagt tcaacgaagt tcggcggagt aacggagatc
     7981 ggctgaccat tccacagagt tacgatacac tctgcctgat ccagcagcca tttagcattg
     8041 tcgccaattg cttttgcatc agcagacagc tgctcgaaag tttcgttgtt catgaagtgc
     8101 cagaactcac cgtcgttgta caggtaagtc aggttcatat cgacaacatc agcgccttca
     8161 gcggaatcag tagatttgaa ggttttttct acgcgagtac cggtcagcag acgacgcagt
     8221 ttaacgcgag caaatgcctg gcctttaccc ggttttacga attcactcgc ttcaaccgcg
     8281 taaggttcgc cgtctaacat gattttaaga ccagcacgaa aatcgttgct atagtacgtt
     8341 gccataaggc cctctgaaat ttgttaattg gtagctaagc cacaaaatgg cgcatattgt
     8401 aaccctaaat accccatcca gagaagattg gttaacgcaa cttgccgatg ttgtgaccga
     8461 tcctgatgaa cttctgcgtc ttttgaatat agacgcggac gaaaaactgt tggccggacg
     8521 cagcgccaaa aagctgtttg ccctgcgtgt gccccgctca tttatcgatc gcatggagaa
     8581 aggcaatccg gacgatcctc ttttgcgtca ggtacttacc tcgcaagatg agtttgtcgt
     8641 cgcgtccgga ttctccaccg acccactgga agaacagcac agcgtagtgc ctggtttgtt
     8701 gcataaatac cacaaccggg cgcttttgct ggtcaaaggc ggctgcgcgg taaattgccg
     8761 ctattgcttc cgtcgccact tcccctatgc cgaaaatcag ggcaacaagc gtaactggca
     8821 gactgcactt gagtatgttg ctgcgcatcc ggaactggac gagatgattt tctccggcgg
     8881 cgatccgctg atggcgaaag agcacgagct ggactggttg ctcacacaac tggaagccat
     8941 cccgcatatc aaacgtctgc ggattcacag ccgtctgccg attgtgatcc cggcacgtat
     9001 caccgaggcg ctggttgaac gctttgcccg ttctacgctg caaatcttgc tggtgaatca
     9061 catcaatcat gccaatgagg tagatgaaac gttccgtcag gcgatggcta aattgcgccg
     9121 tgtcggtgtc accctgctta accagagcgt tctgttacgt ggtgtgaacg ataacgcaca
     9181 aacgttggca aacctgagta atgcgttgtt cgatgccggc gtgatgccgt attacctgca
     9241 tgtgctagat aaagtacagg gcgcggcgca ttttatggtg agtgatgacg aagcacggca
     9301 gattatgcgt gagttgctga cattggtgtc gggatatctg gtgccgaaac tggcgcgaga
     9361 aattggcggc gaacccagca aaacgccgct ggatctccag ctacgccagc agtaactgat
     9421 taaacaggga agttaaacat taaaattgcc tgatgcgcta cgcttatcag gcctacatcg
     9481 tgcattgaaa ttgaatggat ttgcaatgaa ctgtaggccg gataaggcgt ttacgccgca
     9541 tccggcaaga gcaacaaacg caagtacagc aaccactgag aaatttaatc tttgcacgtt
     9601 gcataacctt taattattca tatgcattgc tgttgttact ttttatatag ctaacaccaa
     9661 taattaacct ttcttttcgg taatgactcc aacttactga tagtgtttta tgt
//
LOCUS       DAETQL010000350         8852 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.guided.141, whole genome shotgun sequence.
ACCESSION   DAETQL010000350 DAETQL010000000
VERSION     DAETQL010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.guided.141"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            264..923
                     /locus_tag="K0W65_004557"
     CDS             264..923
                     /locus_tag="K0W65_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HBI3097478.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1124..1501)
                     /locus_tag="K0W65_004558"
     CDS             complement(1124..1501)
                     /locus_tag="K0W65_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBI3097479.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1568..4533)
                     /locus_tag="K0W65_004559"
                     /pseudo
     CDS             complement(1568..4533)
                     /locus_tag="K0W65_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
     gene            complement(4536..5096)
                     /locus_tag="K0W65_004560"
     CDS             complement(4536..5096)
                     /locus_tag="K0W65_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBI3097480.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(5222..5572)
                     /locus_tag="K0W65_004561"
     CDS             complement(5222..5572)
                     /locus_tag="K0W65_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HBI3097481.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="K0W65_004562"
     CDS             complement(5775..6788)
                     /gene="intI1"
                     /locus_tag="K0W65_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HBI3097482.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            6998..7828
                     /locus_tag="K0W65_004563"
     CDS             6998..7828
                     /locus_tag="K0W65_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HBI3097483.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            7941..8732
                     /gene="aadA1"
                     /locus_tag="K0W65_004564"
     CDS             7941..8732
                     /gene="aadA1"
                     /locus_tag="K0W65_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBI3097484.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT         2016 a         2367 c         2497 g         1972 t
ORIGIN      
        1 acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt tgcgccgaat
       61 aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc cctgttgata
      121 ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac gtaagaggtt
      181 ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag ttatcgagat
      241 tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc accgttgata
      301 tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct caatgtacct
      361 ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag aaaaataagc
      421 acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct catccggaat
      481 tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac ccttgttaca
      541 ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac cacgacgatt
      601 tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa aacctggcct
      661 atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc tgggtgagtt
      721 tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc gttttcacca
      781 tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt caggttcatc
      841 atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa cagtactgcg
      901 atgagtggca gggcggggcg taattttttt aaggcagtta ttggtgccct taaacgcctg
      961 gttgctacgc ctgaataagt gataataagc ggatgaatgg cagaaattcg aaagcaaatt
     1021 cgacccggtc gtcggttcag ggcagggtcg ttaaatagcc gcttatgtct attgctggtt
     1081 taccggttta ttgactaccg gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca
     1141 gtttgctcag gctctccccg tggaggtaat aattgacgat atgatcattt attctgcctc
     1201 ccagagcctg ataaaaacgg ttagcgccgg ggttggattt ttcagcgttc cagctaaggc
     1261 taaggcattc ctgttcaagc gcaagccggg ctataaagcg cattatcgct ttacccgtgc
     1321 ccttatttcg atcgcactga gaaacataca gctctttaat atgcagctga ccgctgtatc
     1381 ggggcgaggg ataaagaata ttgcaacatg ccaggccagt aatgttattg ccgcagcgcg
     1441 ctctgatcac cagggtaccg gaaagccggt tgaataactt tttacagaga taatccttca
     1501 tcaacgcttc ctgaatgata ccttcgccat aataggggtc gtctcagaaa acggaaaata
     1561 aagcacgcta agccggttgc agaggccgta gcggcctgaa cttccccgcg ccgatcttgg
     1621 cgctgctgcg ccataggtaa tcaccggtca ggttgatgtg ctcccagccg agtggcgaca
     1681 ggtactgcaa tagcgagtca tcgacggcat gaccattgcc gcgcaacgca tgcgccgcac
     1741 gctccaggta gaccgtgttc cacagcacga tggccgccgt caccaggttg aggccgctgg
     1801 cccggtagcg ctgctgctcg aaactgcggt cacggatttc accaaggcgg ttgaagaaca
     1861 cggcacgggc cagcgcattg cgcgcctcgc ccttgttcag cccggcatgc acgcggcggc
     1921 gtagctcgac gctttgcagc cagtcgagga tgaacagcgt gcgctcgatg cggcccaact
     1981 cgcgcagcgc gacggccaag ccgttctggc gcgggtagct gccgagtttc ctgagcatca
     2041 gcgaggccgt caccgtgccc tgcttgatcg aggtggccag ccgcaggatt tcgtcccaat
     2101 gggcgcggac gtgcttgatg ttgagcgtgc cgccgatcat cggcttgagc gcgtcatagg
     2161 cggcatcgcc cttcgggatg tagagcttgg tgtcgcccag gtcgcggatg cgcggcgcga
     2221 agcggaagcc caagaggtgc atcagggcga agacgtgatc ggtgaagccc gccgtgtcgg
     2281 tgtagtgctc ctcgatccgc aggtcggatt cgtggtacag caggccgtcg agcacgtagg
     2341 ttgagtcgcg caggccgaca ttgaccacct tggtgtggaa tggcgcgtat tggtcggaga
     2401 tgtgggtgta gaaagtccgt cctgggctgc tgccatattt tgggttgatg tgccccgtgc
     2461 tctttgcctt gctagcggtt cggaaattct gtccgtccga tgatgatgtg gtgccatcgc
     2521 cccagtgccc ggcaaaggga tgccgaaact gagcgttgac cagttcagcc aacgctgtcg
     2581 agtacgtttc gtcgcgggta tgccaggctt gcagccaagc gagcttcgcg taggtcgtgc
     2641 cggggcagga ctcggccatc ttggtcaggc ccaggttgat cgcgtcggcc aggatcgtgg
     2701 tcaacaacag gttcttgtcc ttggccagat cgcccgattt caagtgcgtg aagtgccggg
     2761 tgaagcccgt ccactcatcg acttcgagca gcagttcggt gatcttgacg tgcggcagga
     2821 ccatggctgt ctggtctatc agcgcctgcg cggtgtcggg caccgccgca tccagcggcg
     2881 tgatcttcaa gcccgactcg gtgatgatgg catccggcag gtcgttggct gccgccatgc
     2941 ggttgacggt ggcaagttgt gcttccagca gcgtcagccg ctcatgcaga tattgttcgc
     3001 agtcggtggc cacggccagc ggcaattcgc tggactgctt gaggctggtg aacttctcgg
     3061 gcggtaccag gtagtcctcg aagtccttga actggcgtga accctgcacc cagatgtcgc
     3121 ccgagcgcag ggagttcttc aactcggaca gcgcgcacag ttcgtagtag cgccggtcga
     3181 tgccggcgtc ggtcatcacc agtttctgcc agcgcggctt gatgaagccg gtcggtgcat
     3241 cggctggcag ctgcgggcgt tgtcggtgtt catgccgcgc agcacctcaa tggcatcaag
     3301 cacgtttttg gcggcgggcg cggcccgcag cttgagcacg gcaaggaatt ccggtgcata
     3361 gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg aaatcgaagt catcgggttg
     3421 cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa tcccaggaca tgacggcctc
     3481 gatggcggca aacgcatcgc ggcctgattg cttggcgtcg atcagcgcct gaccgatgcg
     3541 cccgtacaga cgtaccttgg cgttgatggc cttgcctgac gcctggaact gctgctgatg
     3601 cttattcttg gcagcgttaa acagcttacc caggatgcgg tcgtgcaggt cgatgatttc
     3661 gtcggtgacg gtggccatgc cctcggtggc cagcgccacg agagtggcgt agcgccgttg
     3721 cggctcgaat ttggccaggt cggcgggtgt catctggccg ccctcgcggg caatcttgag
     3781 caggcggttc tggtgaacca gccgctcgat gccggtaggc agatcgagtg cctgccatgc
     3841 cttgaggcgt tcgatgtgtt ccagcatatg ccgcgaattt ggcttggccg gagactggcg
     3901 caaccaagcc aaccaggtcg tcttgccgtt gtcccggcgc ttgagcagat cgtcgaggcg
     3961 gcggcgatgc gcgtccgcca gtggttcggc caaggcgtcg tagatgcgcc ggttagcacg
     4021 ggtgatcgcc tcggcactcg cccgctcgac ggcgttgagg gcgggcagaa tgaccgactg
     4081 ccgccgcagg tgcccgatca aggcgctggc cagcacgatg cctttgtcgg tttgcatcgc
     4141 cagctcggtc agcatctgga cggcctgccg gtaatggctc atggtgaagg gccggaaacc
     4201 gaacacggtt tgcagctcgc tcaggtgctc gcgccgggtc tgctcccgct ggccgtactc
     4261 gttccagctt tcgacgccga ccttgagctg gtcggcgacc agcttcaaca agggcgggaa
     4321 cggtagttca tcgacgccca ggatgacgcc gggaaagcgc aggtaacaga gctgcaccgc
     4381 gaagcccagc cgattggctg gcccgcgccg ctgtcggatg atcgagaggt cggtatcgtt
     4441 gaatgtgtaa tgtcggatca ggtcgtcctt ggagtccggc aacgccagca ggctttcccg
     4501 ctcggcggcg gacaggatgg aacgacgtgg catatttact gatccgttct caagtattga
     4561 tacagggttt cgcgactgat tccgaattca cgagcaagct tggtcttttg ctcgccagcc
     4621 tcgacacgtt ggcgcagttc ggcaatacgc tcagacgaca gggatttctt cctgccacgg
     4681 taagccccgc gttgcttggc gagcgcaata ccctcgcgct gacgctcgcg gatcagggcg
     4741 cgctcgaact cggcgaacgc gcccatcacc gagagcatca ggttcgccat cggagagtct
     4801 tcgccagtaa aactgaggtg ttccttgacg aattcgatat gcacgccgcg ttgtgtcagc
     4861 gtttgcacga tccggcgcaa atcatcgaga ttgcgcgcca ggcgatccat gctatgcacc
     4921 accacggtgt cgccggtgcg ggcgaagctt atcagcgctt ccagttgcgg acgcttgaca
     4981 tccttgccgg atgccttgtc gctaaaagcg cgatcaacct tgacgccttc cagttgccgt
     5041 tccgggttct ggtcgaaggt gctgaccctg atatacccaa tgcgctgtcc agtcatggaa
     5101 ttccctgcaa aatgtcaggg aagactctat gaccttcaac gagatatgtc aataaattca
     5161 aaattcaatc ctatcctgac gcaatttaca catggcatct gacatcaggt tagggtatgc
     5221 ctcaacctga cggcggcgaa tcacaagcgt ccggtttgac gctggtttcg gtcgcagtgc
     5281 tggcccgcgc ctggaagcgc tgatagcact ccagcccgca gaaatgctcc acgtactcgg
     5341 ccccttccgg cgtgaaggcg gcatcgagcg ggatttcctt gcagcacacg cagcaactgg
     5401 tgctcggttc attggcgttc atggtggtgt tcctccattg gttgacgaag ccgacgaagg
     5461 ccgccgccgg catcggcctg gcgaacagga aaccctgcac cgtgtcgcaa cccgcctgcc
     5521 gcaaccacgc aaggcagtcg ggtgtttcca caccctcggc taccacctcc attgtcgttt
     5581 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
     5641 atccatcagg caacgacggg ctgctgccgg ccatcagcgg acgcagggag gactttccgc
     5701 aaccggccgt tcgatgcggc accgatggcc ttcgcgcagg ggtagtgaat ccgccaggat
     5761 tgacttgcgc tgccctacct ctcactagtg aggggcggca gcgcatcaag cggtgagcgc
     5821 actccggcac cgccaacttt cagcacatgc gtgtaaatca tcgtcgtaga gacgtcggaa
     5881 tggccgagca gatcctgcac ggttcgaatg tcgtaaccgc tgcggagcaa ggccgtcgcg
     5941 aacgagtggc ggagggtgtg cggtgtggcg ggcttcgtga tgcctgcttg ttctacggca
     6001 cgtttgaagg cgcgctgaaa ggtctggtca tacatgtgat ggcgacgcac gacaccgctc
     6061 cgtggatcgg tcgaatgcgt gtgctgcgca aaaacccaga accacggcca ggaatgcccg
     6121 gcgcgcggat acttccgctc aagggcgtcg ggaagcgcaa cgccgctgcg gccctcggcc
     6181 tggtccttca gccaccatgc ccgtgcacgc gacagctgct cgcgcaggct gggtgccaag
     6241 ctctcgggta acatcaaggc ccgatccttg gagcccttgc cctcccgcac gatgatcgtg
     6301 ccgtgatcga aatccagatc cttgacccgc agttgcaaac cctcactgat ccgcatgccc
     6361 gttccataca gaagctgggc gaacaaacga tgctcgcctt ccagaaaacc gaggatgcga
     6421 accacttcat ccggggtcag caccaccggc aagcgccgcg acggccgagg tcttccgatc
     6481 tcctgaagcc agggcagatc cgtgcacagc accttgccgt agaagaacag caaggccgcc
     6541 aatgcctgac gatgcgtgga gaccgaaacc ttgcgctcgt tcgccagcca ggacagaaat
     6601 gcctcgactt cgctgctgcc caaggttgcc gggtgacgca caccgtggaa acggatgaag
     6661 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     6721 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     6781 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     6841 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     6901 cagcagggca gtcgccctaa aacaaagttg ggcgaacccg gagcctcatt aattgttagc
     6961 cgttaaaatt aagcccttta ccaaaccaat acttattatg aaaaacacaa tacatatcaa
     7021 cttcgctatt tttttaataa ttgcaaatat tatctacagc agcgccagtg catcaacaga
     7081 tatctctact gttgcatctc cattatttga aggaactgaa ggttgttttt tactttacga
     7141 tgcatccaca aacgctgaaa ttgctcaatt caataaagca aagtgtgcaa cgcaaatggc
     7201 accagattca actttcaaga tcgcattatc acttatggca tttgatgcgg aaataataga
     7261 tcagaaaacc atattcaaat gggataaaac ccccaaagga atggagatct ggaacagcaa
     7321 tcatacacca aagacgtgga tgcaattttc tgttgtttgg gtttcgcaag aaataaccca
     7381 aaaaattgga ttaaataaaa tcaagaatta tctcaaagat tttgattatg gaaatcaaga
     7441 cttctctgga gataaagaaa gaaacaacgg attaacagaa gcatggctcg aaagtagctt
     7501 aaaaatttca ccagaagaac aaattcaatt cctgcgtaaa attattaatc acaatctccc
     7561 agttaaaaac tcagccatag aaaacaccat agagaacatg tatctacaag atctggataa
     7621 tagtacaaaa ctgtatggga aaactggtgc aggattcaca gcaaatagaa ccttacaaaa
     7681 cggatggttt gaagggttta ttataagcaa atcaggacat aaatatgttt ttgtgtccgc
     7741 acttacagga aacttggggt cgaatttaac atcaagcata aaagccaaga aaaatgcgat
     7801 caccattcta aacacactaa atttataaaa aatctaatgg caaaatcgcc caacccttca
     7861 atcaagtcgg gacggccaaa agcaagcttt tggctcccct cgctggcgct cggcgcccct
     7921 tatttcaaac gttaaacatc atgagggaag tggtgatcgc cgaagtatcg actcaactat
     7981 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt
     8041 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg
     8101 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt
     8161 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg
     8221 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc
     8281 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct
     8341 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct
     8401 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga
     8461 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt
     8521 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg
     8581 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac
     8641 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtt cactacgtga
     8701 aaggcgagat caccaaggta gtcggcaaat aatgtctaac aattcgttca agccgacgcc
     8761 gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt gcggtgatgc
     8821 tgccaactta ctgatttagt gtatgatggt gt
//
LOCUS       DAETQL010000351         7053 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.guided.160, whole genome shotgun sequence.
ACCESSION   DAETQL010000351 DAETQL010000000
VERSION     DAETQL010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.guided.160"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            59..1102
                     /locus_tag="K0W65_004565"
     CDS             59..1102
                     /locus_tag="K0W65_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBI3097485.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            1164..1778
                     /locus_tag="K0W65_004566"
                     /pseudo
     CDS             1164..1778
                     /locus_tag="K0W65_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="K0W65_004567"
     CDS             complement(1835..3292)
                     /gene="pepD"
                     /locus_tag="K0W65_004567"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBI3097486.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3554..4012
                     /gene="gpt"
                     /locus_tag="K0W65_004568"
     CDS             3554..4012
                     /gene="gpt"
                     /locus_tag="K0W65_004568"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBI3097487.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4104..5348
                     /gene="frsA"
                     /locus_tag="K0W65_004569"
     CDS             4104..5348
                     /gene="frsA"
                     /locus_tag="K0W65_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBI3097488.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            5406..5807
                     /gene="crl"
                     /locus_tag="K0W65_004570"
     CDS             5406..5807
                     /gene="crl"
                     /locus_tag="K0W65_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBI3097489.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="K0W65_004571"
     CDS             complement(5846..6901)
                     /gene="phoE"
                     /locus_tag="K0W65_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBI3097490.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         1716 a         1735 c         1817 g         1785 t
ORIGIN      
        1 aattcaagtc gcagcacatg caacttgaag tatgacgagt ctatttacgg aatggtatat
       61 gggcaattat attcgtccct tatccgatgc ggtatttacc atcgcatctg atgacctgtg
      121 gatcgagagt ttagcgatcc aacaattaca caccacggca aatttaccca acatgcagcg
      181 cgtagtgggg atgccagatt tacaccccgg acgcggctac ccgattggcg cagcgttctt
      241 ctccgcaggc cgtttttacc cagcactggt cggcaatgat atcggctgtg gcatggcgct
      301 atggcaaaca gatattctcg ctcgcaaata caacgccgat aagtttgaaa agcgattatc
      361 agctttggat gacgttgctg aagaaagctg gctggaggaa aacctgccgt cagcatttgc
      421 acagcatccg tggcgcagct cgcttggctc tatcggtggt ggcaatcatt tcgctgaact
      481 gcaacaagtt gatcaaatca tcaacgctga actgtttgca ctggcaggtc tggatgcgca
      541 gcatctgcaa ctgctggttc atagcggctc gagggggcta gggcagtcta ttttacagcg
      601 gcatattgcc tcgttttcgc atcatggttt gcctgaaggc agtgacgacg cgctggcgtt
      661 tgcgcgtttt aatcgccacc tgatcgcttt gcgcataatg caacaggtta aggccaccgg
      721 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcgaatcg gtgatcaaca
      781 aggggtctta catcgtaaag gtgccacgcc tgatgactgt ggtctggtgg ttattcccgg
      841 ttcacgcggt gattactcca ggctggttca gcccgtcagg agtgaggaaa cattgcattc
      901 gctggcgcat ggggctgggc gtaaatgggg gcgcaccgag tgtaaagggc gtctggcagc
      961 gaaatacaca gcgacgcaac tctcgcgtac tgaacttggc agtcgggtaa tttgtcgcga
     1021 taaacaactc atcttcgaag aagcgccaca agcctataaa tcggctgaaa gtgtggtgca
     1081 atgtcagggc aggccggatt aattattcct gtcgcgcggc tgcgtccggt gctaacgctc
     1141 aaaaacagtg gagggaaaaa aggatgatct tgctataact ctcctcagcg caggggccgg
     1201 aagaatgttg tctcgcagtg aaaaaggcgc tggacaggct aattaaagaa gctgcccgac
     1261 aagacgtcgc ggtaacggtg ctggaaacag aaacaggccg ctactctgac acgctgcgtt
     1321 cggcgctgat ttctctggat ggcgacaacg catgggcgtt aagcgaaagc tggtgtggca
     1381 ctattcagtg gatttgcccg agcccgtatc gcccacatca cgggcgtaaa aactggtttc
     1441 tgggcattgg gcgttttacc gctgatgagc aggagcaatc cgatgcgatc cggtatgaga
     1501 cgctgcgttc gtcggggccg ggcggtcaac atgtcaataa aaccgactcg gcggtacgcg
     1561 ccacgcattt ggcatccggt attagcgtga aggttcagtc agagcgcagc cagcatgcta
     1621 acaaacggct ggcgcgattg ctgattgcct ggaagctgga tcagcagcaa taggaaaata
     1681 gcgcggtgct gaaatcacag cggcgaatgt tccatcacca gattgaacgt ggcaacccgc
     1741 gacgaacgtt tacagggatg gcttttatcg aaggataatg aaggatgaaa ctgccggaag
     1801 gcgattaaac gccatccggc agcaaatcaa ataattactt cgccggaatt tctttcagca
     1861 gttcagtcag cagtgtccag taatgaccta cgctttcgat gtgaacttgc tcatccggag
     1921 agtgtggacc ggtgatagtt ggcccgatag aaaccatgtc catttccgga tacggttttt
     1981 tgaacagacc acattccagg cccgcgtgga taatctggat gttcggcgtc ttgttgaaca
     2041 ggcgctgata ggtttcacgt accagatgca tcaccggaga attagcgtcc ggctgccagc
     2101 caggatatgc gcctttcgct tcggttttcg cgccagccag tttacccagc gaatccagca
     2161 tgctcaccac gtagtcttta ccgctgtcga ttagtgaacg gatcaggcag tgaatttcta
     2221 cgttattgtc agtcatggtc accacaccga cgttcaggga ggtttcgacc acaccttttg
     2281 ccacgtcgga attgcggatc acaccgttcg gggtggcgtt cagcagacga ataaaggtat
     2341 cgcgagattt cgcaatcagg gcagctttat cgttcgctac agagtccagc aacaaggcca
     2401 gatttttctc tttctctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag
     2461 atttcagtgc gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga
     2521 tggcgttacg cagtgtgccg ccgttgaaat cgataaggcg caggtccagt tcttctgcat
     2581 gacccgccag gaagcgcacc agcagtttgt tggcattacc caggccaacg tggatttcac
     2641 cgccggagtg accgcctttc agacctttta aggttaactt gaaggtttca aaaccagctg
     2701 gaaccgcttc acgatctaaa tgcaggttgg aggtgaagtc gatacccccc gcacaaccca
     2761 tgtagatttc accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt
     2821 tgctctgtaa gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt
     2881 ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc cagtgcagag gccataccaa
     2941 tgccgttatc cgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag
     3001 gctggatagg atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat
     3061 cgaggtgggc ctgtaagacg accggtttac gattttccat acctgcggta gcaggttgac
     3121 gaatcaggat attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac
     3181 caacaatgta ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt
     3241 tggcaaaaat atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc
     3301 tccttgtcga tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc
     3361 aacacaagac aggcttgcga gatatgtatt gagaatacca ctttatcccg cgtcagggag
     3421 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     3481 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     3541 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     3601 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     3661 ccgtggcggt ctggtaccgg gggcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     3721 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     3781 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     3841 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     3901 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggatcga
     3961 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     4021 acgcctggca ttgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
     4081 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     4141 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     4201 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     4261 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
     4321 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgataccgtg attggctacc
     4381 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
     4441 gtacggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
     4501 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
     4561 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
     4621 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
     4681 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
     4741 tgtatgaacg ttattttgcg ccgcgcggca ttgcgatgct gactattgat atgccgtcgg
     4801 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
     4861 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
     4921 gtttcggcgc taacgttgcc gtgcgtctgg cataccttga atcgccgcgt ctgaaagcgg
     4981 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
     5041 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
     5101 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctggggcgtc
     5161 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
     5221 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
     5281 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
     5341 tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac agttgcatca caacaggaga
     5401 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
     5461 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
     5521 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
     5581 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
     5641 caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga ctggagcaca
     5701 ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg
     5761 aaccggcgaa tgattttcgt gatgaaccgg tgaagttaac ggcgtgagtg aaatgtgccg
     5821 gatgcatcac atccggcaat attcattaaa actgatacgt catcccaacc gcgacagtat
     5881 catcattatt aatattcagc ttattatcgc tatccagttg gttgatttta taatcaacaa
     5941 acgctgacat atttttgttg aaataatacg tagcaccgac gtcgatataa ttgaccagat
     6001 cttcatcacc gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac
     6061 gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa
     6121 agccgccagt tattggcgtc attttgcgtg tttcagaata gaaagttgcc agataaatat
     6181 tattggcatc gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc
     6241 tttgcaggtt ctgctcgttg gtgcgatctg agttggtata ggccccacta atggcgaaat
     6301 cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa
     6361 cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc
     6421 cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc gcgtttggtc ataaagttgt
     6481 cggtctgcgc cgaggaatcg ccaccaaatt ccgggaacat atcggtccag gcttccacgt
     6541 catacaacgc gcccaggtta cgaccataat cgaaagaacc caaatcttta tatttcaacc
     6601 cggcaaaagc gagacgcgtt ttttgctgtg cagtatcact ctccgcttta tttccggcaa
     6661 actccgcttc ccaccgacca taaccagtca gttgatcgtt aatttgagtt tcacctttaa
     6721 aaccaaaacg gatataactc tggtcgccat ctttactggc gttatcactc atataatgca
     6781 tggctttaac tttgccatag acatccagtt tattaccgtc tttattatat atttctgcag
     6841 cctgtacaga tgcagatgcc acaatgccca tcaccactaa tgccagagtg ctctttttca
     6901 ttttcattcc tgattttaat taacgcgcga atattcagcg ggagagcccc gttgaaaaca
     6961 ggaaagtttt taacctgaga ttgttaaaga tatattacag attaatgata ttctgaaaat
     7021 gggtaatgct gccaacttac tgatttagtg tat
//
LOCUS       DAETQL010000352         5664 bp    DNA     linear   BCT 29-JUL-2021
DEFINITION  TPA_asm: Shigella flexneri isolate SH180
            SAMN15686862-rid12761743.guided.174, whole genome shotgun sequence.
ACCESSION   DAETQL010000352 DAETQL010000000
VERSION     DAETQL010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15686862
            Sequence Read Archive: SRR12358861
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JUL-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-JUL-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001097959.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001097959.1
            Genome Coverage       :: 90x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/29/2021 01:18:39
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,579
            CDSs (total)                      :: 4,481
            Genes (coding)                    :: 4,051
            CDSs (with protein)               :: 4,051
            Genes (RNA)                       :: 98
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 430
            CDSs (without protein)            :: 430
            Pseudo Genes (ambiguous residues) :: 0 of 430
            Pseudo Genes (frameshifted)       :: 190 of 430
            Pseudo Genes (incomplete)         :: 138 of 430
            Pseudo Genes (internal stop)      :: 155 of 430
            Pseudo Genes (multiple problems)  :: 52 of 430
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15686862-rid12761743.guided.174"
                     /isolate="SH180"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Spain: Barcelona"
                     /lat_lon="41.428083 N 2.142278 E"
                     /collection_date="2018"
                     /collected_by="Hospital Universitari Vall d'Hebron"
     gene            70..285
                     /locus_tag="K0W65_004572"
     CDS             70..285
                     /locus_tag="K0W65_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBI3097491.1"
                     /translation="MSIERFQSLATEGKMLSLSWWENEYAVLQWKNHVLHAKAQQEGR
                     ESIFDFYKISIAHITREYSFKKDKDNV"
     gene            278..664
                     /locus_tag="K0W65_004573"
     CDS             278..664
                     /locus_tag="K0W65_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HBI3097492.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            672..1358
                     /locus_tag="K0W65_004574"
     CDS             672..1358
                     /locus_tag="K0W65_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBI3097493.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="K0W65_004575"
     CDS             complement(1336..1959)
                     /gene="tetR(B)"
                     /locus_tag="K0W65_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HBI3097494.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2041..3246
                     /gene="tet(B)"
                     /locus_tag="K0W65_004576"
     CDS             2041..3246
                     /gene="tet(B)"
                     /locus_tag="K0W65_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HBI3097495.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="K0W65_004577"
     CDS             complement(3359..3952)
                     /gene="tetC"
                     /locus_tag="K0W65_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HBI3097496.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4085..4456
                     /locus_tag="K0W65_004578"
     CDS             4085..4456
                     /locus_tag="K0W65_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBI3097497.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4466..>5664)
                     /locus_tag="K0W65_004579"
     CDS             complement(4466..>5664)
                     /locus_tag="K0W65_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HBI3097498.1"
                     /translation="HKTLSVSWSHYPELHLKRLNSLTLACHALLDCKTLTLTELGRNL
                     PTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKR
                     LMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGF
                     KVPWYKSVEKLGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKS
                     NPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQ
                     LVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVH
                     AQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHG
                     YALGKL"
BASE COUNT         1568 a         1044 c         1230 g         1822 t
ORIGIN      
        1 tctgaacata aaacactatc aataagttgg agtcattacc gaccactatt aagtcatgta
       61 gctggtttta tttcaattga acgttttcaa agtctagcta cagaaggaaa aatgttatcg
      121 ctatcttggt gggaaaacga atacgcagtt ctgcaatgga aaaatcatgt tttacatgcg
      181 aaagctcaac aagaagggcg agagtcaata tttgattttt acaaaattag tattgctcat
      241 attactcgcg aatattcatt taaaaaggac aaggataatg tttgatgttc acgttgtttt
      301 agataatcaa ataggacaat tagcattact aggaaaaaca ttaggtaata aaggtattgg
      361 attggaaggg ggagggatat ttacggttgg tgatgaatgc catgctcatt ttcttgttga
      421 acaaggaaag gaagctaaaa tagcgctaga gcaagctgga ctgttagtac ttgcgatccg
      481 gacaccatta attcgtaagt taaaacagga aaaaccgggg gaacttggcg aaatagcacg
      541 agtattggcg gagaataaca ttaatatttt agtgcaatac agtgaccatg ctaaccaact
      601 gatattaata acggacaatg atagtatggc tgcatctgtt acgctccctt gggcaataaa
      661 gtgaacttgc gatggctaat ttaatacgaa aagaggttac ctttgagtcc tcaatagccg
      721 cgataggggc ggctatgtct gacatttcac gagttaaaat actcagtgct ttgatggatg
      781 ggcgagcttg gacggccact gagctaagtt ctgtggcgaa tatatcagct tcaacggcga
      841 gcagtcattt atctaaatta ttagattgcc agctaatcac agtagtagct caaggcaagc
      901 atcgttattt tcggctagca ggaaaagata ttgctgaatt gatggaaagt atgatgggga
      961 tctccttaaa ccatggcgta catgccaaag tttccacgcc agtgcattta cgaaaagcac
     1021 gtacttgcta tgatcattta gctggcgaag ttgccgttaa gatctatgat tccctttgtc
     1081 aacagcaatg gatcactgaa aatggttcaa tgatcacatt aagtggtatt caatattttc
     1141 atgaaatggg aattgacgtt ccttccaaac attcacgtaa aatctgttgt gcgtgtttag
     1201 attggagtga acgccgtttc catttaggtg ggtacgttgg agccgcatta ttttcgcttt
     1261 atgaatctaa agggtggtta actcgacatc ttggttaccg tgaagttacc atcacggaaa
     1321 aaggttatgc tgcttttaag acccactttc acatttaagt tgtttttcta atccgcatat
     1381 gatcaattca aggccgaata agaaggctgg ctctgcacct tggtgatcaa ataattcgat
     1441 agcttgtcgt aataatggcg gcatactatc agtagtaggt gtttcccttt cttctttagc
     1501 gacttgatgc tcttgatctt ccaatacgca acctaaagta aaatgcccca cagcgctgag
     1561 tgcatataat gcattctcta gtgaaaaacc ttgttggcat aaaaaggcta attgattttc
     1621 gagagtttca tactgttttt ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg
     1681 atgacttagt aaagcacatc taaaactttt agcgttatta cgtaaaaaat cttgccagct
     1741 ttccccttct aaagggcaaa agtgagtatg gtgcctatct aacatctcaa tggctaaggc
     1801 gtcgagcaaa gcccgcttat tttttacatg ccaatacaat gtaggctgct ctacacctag
     1861 cttctgggcg agtttacggg ttgttaaacc ttcgattccg acctcattaa gcagctctaa
     1921 tgcgctgtta atcactttac ttttatctaa tctagacatc attaattcct aatttttgtt
     1981 gacactctat cattgataga gttattttac cactccctat cagtgataga gaaaagtgaa
     2041 atgaatagtt cgacaaagat cgcattggta attacgttac tcgatgccat ggggattggc
     2101 cttatcatgc cagtcttgcc aacgttatta cgtgaattta ttgcttcgga agatatcgct
     2161 aaccactttg gcgtattgct tgcactttat gcgttaatgc aggttatctt tgctccttgg
     2221 cttggaaaaa tgtctgaccg atttggtcgg cgcccagtgc tgttgttgtc attaataggc
     2281 gcatcgctgg attacttatt gctggctttt tcaagtgcgc tttggatgct gtatttaggc
     2341 cgtttgcttt cagggatcac aggagctact ggggctgtcg cggcatcggt cattgccgat
     2401 accacctcag cttctcaacg cgtgaagtgg ttcggttggt taggggcaag ttttgggctt
     2461 ggtttaatag cggggcctat tattggtggt tttgcaggag agatttcacc gcatagtccc
     2521 ttttttatcg ctgcgttgct aaatattgtc gctttccttg tggttatgtt ttggttccgt
     2581 gaaaccaaaa atacacgtga taatacagat accgaagtag gggttgagac gcaatcgaat
     2641 tcggtataca tcactttatt taaaacgatg cccattttgt tgattattta tttttcagcg
     2701 caattgatag gccaaattcc cgcaacggtg tgggtgctat ttaccgaaaa tcgttttgga
     2761 tggaatagca tgatggttgg cttttcatta gcgggtcttg gtcttttaca ctcagtattc
     2821 caagcctttg tggcaggaag aatagccact aaatggggcg aaaaaacggc agtactgctc
     2881 ggatttattg cagatagtag tgcatttgcc tttttagcgt ttatatctga aggttggtta
     2941 gttttccctg ttttaatttt attggctggt ggtgggatcg ctttacctgc attacaggga
     3001 gtgatgtcta tccaaacaaa gagtcatcag caaggtgctt tacagggatt attggtgagc
     3061 cttaccaatg caaccggtgt tattggccca ttactgtttg ctgttattta taatcattca
     3121 ctaccaattt gggatggctg gatttggatt attggtttag cgttttactg tattattatc
     3181 ctgctatcga tgaccttcat gttaacccct caagctcagg ggagtaaaca ggagacaagt
     3241 gcttagttat ttcgtcacca aatgatgtta ttccgcgaaa tataatgacc ctcttgataa
     3301 cccaagaggg cattttttac gataaagaag atttagcttc aaataaaacc tatctatttt
     3361 atttatcttt caagctcaat aaaaagccgc ggtaaatagc aataaattgg ccttttttat
     3421 cggcaagctc ttttaggttt ttcgcatgta ttgcgatatg cataaaccag ccattgagta
     3481 agtttttaag cacatcatca tcataagctt taagttggtt ctcttggatc aatttgctga
     3541 caatggcgtt taccttacca gtaatgtatt caaggctaat tttttcaagt tcattccaac
     3601 caatgatagg catcacttct tggataggga taaggttttt attattatca ataatataat
     3661 caagataatg ttcaaatata ctttctaagg cagaccaacc atttgttaaa tcagtttttg
     3721 ttgtgatgta ggcatcaatc ataattaatt gctgcttata acaggcactg agtaattgtt
     3781 ttttattttt aaagtgatga taaaaggcac ctttggtcac caacgctttt cccgagatct
     3841 catctattga aacagcttga tagccttttt caacaaacaa tattcgtgct gagttaacca
     3901 gtgattgata ggtactctta aaattttctt gttgatgatt tttattttcc atgatagatt
     3961 taaaataaca taccgtcagt atgtttatgg tatcatgatg atgtggtcgt gacaatctta
     4021 agaacattta ggttatttta tgtatattga acagcattct cgctatcaaa ataaagctaa
     4081 taacatccaa ttaagatatg atgataagca gtttcataca acggttatca aagatgttct
     4141 attatggatt gaacataatt tagatcagtc tttactgctt gatgatgtgg cgaataaagc
     4201 gggttatacc aagtggtatt ttcagcggct gttcaaaaaa gtaacagggg tcacactggc
     4261 tagctatatt cgtgctcgtc gtttgacgaa agcggctgtt gagttgaggt tgacgaaaaa
     4321 aactatcctt gagatcgcat taaaatatca atttgattcc caacaatctt ttacacgtcg
     4381 atttaagtac atttttaagg ttacaccaag ttattatcgg cgtaataaat tatgggaatt
     4441 ggaggcaatg cactgagaga tcccctcata atttccccaa agcgtaacca tgtgtgaata
     4501 aattttgagc tagtagggtt gcagccacga gtaagtcttc ccttgttatt gtgtagccag
     4561 aatgccgcaa aacttccatg cctaagcgaa ctgttgagag tacgtttcga tttctgactg
     4621 tgttagcctg gaagtgcttg tcccaacctt gtttctgagc atgaacgccc gcaagccaac
     4681 atgttagttg aagcatcagg gcgattagca gcatgatatc aaaacgctct gagctgctcg
     4741 ttcggctatg gcgtaggcct agtccgtagg caggactttt caagtctcgg aaggtttctt
     4801 caatctgcat tcgcttcgaa tagatattaa caagttgttt gggtgttcga atttcaacag
     4861 gtaagttagt tgctagaacc catggctcct ttgccgacgc tgagtagatt ttaggtgacg
     4921 ggtggtgaca atgagtccgt gtcgagcgct gattttttcg gcctttagag cgagatttat
     4981 acaatagaat ttggcatgag attggattgc ttttagtcag cctcttatag cctaaagtct
     5041 ttgagtgact agatgacata tcatgtaagt tgctgatagg tttccagttt tccgctccta
     5101 ggtctgcata ttgtactttt cctcttactc gacttaacca gtaccaaccc agcttctcaa
     5161 cggatttata ccatggcact ttaaagccag catcactgac aatgagcggt gtggtgttac
     5221 tcggtagaat gctcgcaagg tcggctagaa attggtcatg agctttcttt gaacattgct
     5281 ctgaaagcgg gaacgctttc tcataaagag taacagaacg accgtgtagt gcgactgaag
     5341 ctcgcaatac cataagtcgt ttttgctcac gaatatcaga ccagtcaaca agtacaatgg
     5401 gcatcgtatt gcccgaacag ataaagctag catgccaacg gtatacagcg agtcgctctt
     5461 tgtggaggtg acgattacct aacaatcggt cgattcgttt gatgttatgt tttgttctcg
     5521 ctttggttgg caggttacgg ccaagttcgg taagagtgag agttttacag tcaagtaatg
     5581 cgtggcaagc caacgttaag ctgttgagtc gttttaagtg taattcgggg taatgactcc
     5641 aacttactga tagtgtttta tgtt
//
